LOCUS       DAXWLG010000001        50855 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXWLG010000001 DAXWLG010000000
VERSION     DAXWLG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.001"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            189..419
                     /locus_tag="ACGE4T_000001"
     CDS             189..419
                     /locus_tag="ACGE4T_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFF8385323.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="ACGE4T_000002"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="ACGE4T_000002"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8385324.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="ACGE4T_000003"
     CDS             1830..2006
                     /locus_tag="ACGE4T_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8385325.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="ACGE4T_000004"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="ACGE4T_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFF8385326.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="ACGE4T_000005"
     CDS             2622..3089
                     /locus_tag="ACGE4T_000005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8385327.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="ACGE4T_000006"
     CDS             3174..4058
                     /locus_tag="ACGE4T_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8385328.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="ACGE4T_000007"
     CDS             4172..4408
                     /locus_tag="ACGE4T_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFF8385329.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="ACGE4T_000008"
     tRNA            4496..4576
                     /locus_tag="ACGE4T_000008"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="ACGE4T_000009"
     tRNA            4583..4654
                     /locus_tag="ACGE4T_000009"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            4779..4949
                     /locus_tag="ACGE4T_000010"
     CDS             4779..4949
                     /locus_tag="ACGE4T_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385330.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5169..5876
                     /locus_tag="ACGE4T_000011"
     CDS             5169..5876
                     /locus_tag="ACGE4T_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8385331.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="ACGE4T_000012"
     CDS             complement(5935..6579)
                     /locus_tag="ACGE4T_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFF8385332.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="ACGE4T_000013"
     CDS             6705..6992
                     /locus_tag="ACGE4T_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385333.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="ACGE4T_000014"
     CDS             7100..7420
                     /locus_tag="ACGE4T_000014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8385334.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="ACGE4T_000015"
     CDS             7501..8814
                     /locus_tag="ACGE4T_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFF8385335.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="ACGE4T_000016"
     CDS             8811..9530
                     /locus_tag="ACGE4T_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFF8385336.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="ACGE4T_000017"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="ACGE4T_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFF8385337.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="ACGE4T_000018"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="ACGE4T_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFF8385338.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="ACGE4T_000019"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="ACGE4T_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFF8385339.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="ACGE4T_000020"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="ACGE4T_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFF8385340.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14976..15860
                     /gene="ftsX"
                     /locus_tag="ACGE4T_000021"
     CDS             14976..15860
                     /gene="ftsX"
                     /locus_tag="ACGE4T_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFF8385341.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="ACGE4T_000022"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="ACGE4T_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8385342.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="ACGE4T_000023"
     CDS             17797..18654
                     /locus_tag="ACGE4T_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFF8385343.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="ACGE4T_000024"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="ACGE4T_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFF8385344.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="ACGE4T_000025"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="ACGE4T_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFF8385345.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="ACGE4T_000026"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="ACGE4T_000026"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8385346.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /gene="pstB"
                     /locus_tag="ACGE4T_000027"
     CDS             21410..22168
                     /gene="pstB"
                     /locus_tag="ACGE4T_000027"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8385347.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="ACGE4T_000028"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="ACGE4T_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFF8385348.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23435..24127
                     /locus_tag="ACGE4T_000029"
     CDS             23435..24127
                     /locus_tag="ACGE4T_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8385349.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24124..24891
                     /locus_tag="ACGE4T_000030"
     CDS             24124..24891
                     /locus_tag="ACGE4T_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8385350.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24991..26559
                     /gene="liaX"
                     /locus_tag="ACGE4T_000031"
     CDS             24991..26559
                     /gene="liaX"
                     /locus_tag="ACGE4T_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFF8385351.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26856..27176
                     /locus_tag="ACGE4T_000032"
     CDS             26856..27176
                     /locus_tag="ACGE4T_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFF8385352.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27176..27526
                     /locus_tag="ACGE4T_000033"
     CDS             27176..27526
                     /locus_tag="ACGE4T_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFF8385353.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27693..28631
                     /gene="hprK"
                     /locus_tag="ACGE4T_000034"
     CDS             27693..28631
                     /gene="hprK"
                     /locus_tag="ACGE4T_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFF8385354.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28646..29476
                     /gene="lgt"
                     /locus_tag="ACGE4T_000035"
     CDS             28646..29476
                     /gene="lgt"
                     /locus_tag="ACGE4T_000035"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFF8385355.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29520..30545
                     /locus_tag="ACGE4T_000036"
     CDS             29520..30545
                     /locus_tag="ACGE4T_000036"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8385356.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSRAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30560..31498
                     /gene="galU"
                     /locus_tag="ACGE4T_000037"
     CDS             30560..31498
                     /gene="galU"
                     /locus_tag="ACGE4T_000037"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8385357.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31623..32063
                     /locus_tag="ACGE4T_000038"
     CDS             31623..32063
                     /locus_tag="ACGE4T_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFF8385358.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32066..32668
                     /locus_tag="ACGE4T_000039"
     CDS             32066..32668
                     /locus_tag="ACGE4T_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8385359.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32727..33833)
                     /locus_tag="ACGE4T_000040"
     CDS             complement(32727..33833)
                     /locus_tag="ACGE4T_000040"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFF8385360.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34043..35044
                     /gene="ccpA"
                     /locus_tag="ACGE4T_000041"
     CDS             34043..35044
                     /gene="ccpA"
                     /locus_tag="ACGE4T_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFF8385361.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35381..37726)
                     /locus_tag="ACGE4T_000042"
     CDS             complement(35381..37726)
                     /locus_tag="ACGE4T_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFF8385362.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38005..38907
                     /locus_tag="ACGE4T_000043"
     CDS             38005..38907
                     /locus_tag="ACGE4T_000043"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFF8385363.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39120..40895
                     /locus_tag="ACGE4T_000044"
     CDS             39120..40895
                     /locus_tag="ACGE4T_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFF8385364.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40961..42547)
                     /locus_tag="ACGE4T_000045"
     CDS             complement(40961..42547)
                     /locus_tag="ACGE4T_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFF8385365.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42798..43022
                     /locus_tag="ACGE4T_000046"
     CDS             42798..43022
                     /locus_tag="ACGE4T_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFF8385366.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43163..44914
                     /locus_tag="ACGE4T_000047"
     CDS             43163..44914
                     /locus_tag="ACGE4T_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFF8385367.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44914..46698
                     /locus_tag="ACGE4T_000048"
     CDS             44914..46698
                     /locus_tag="ACGE4T_000048"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385368.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47024..47875
                     /gene="licT"
                     /locus_tag="ACGE4T_000049"
     CDS             47024..47875
                     /gene="licT"
                     /locus_tag="ACGE4T_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8385369.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47971..49851
                     /locus_tag="ACGE4T_000050"
     CDS             47971..49851
                     /locus_tag="ACGE4T_000050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8385370.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49891..50793)
                     /locus_tag="ACGE4T_000051"
     CDS             complement(49891..50793)
                     /locus_tag="ACGE4T_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8385371.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        16449 a         8864 c        10869 g        14673 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagcttc aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca
    13261 gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg
    13321 aagaaatcga attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag
    13381 aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt
    13441 tgttagatgg tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc
    13561 agcatgggta tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga
    13621 gtgtgaccct actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta
    15121 aattagctca agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctaatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcttttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacataaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgagaattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgcg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggactg ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actatcacaa cgatgactgt tgatgcatta
    19321 aaggcagtgc cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggtcttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgattat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctattt
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 gattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagc ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcggaaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cggaagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 tgaagctgtg attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc
    22981 gtttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg
    23041 tttgtgacaa aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
    23101 aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca
    23161 gcatcaagcc gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga
    23221 ggatcatcaa ttgattacag gaaaactgat gaacacggat caaaaaggag aatcgattta
    23281 tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat
    23341 gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg
    23401 gcttttcttc ttcacaagta tcttcattgg cgccatgttg cttattggtg gaaaacaggg
    23461 tctatttgct ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat
    23521 ctttactaaa acagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg
    23581 gatgattatt agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc
    23641 agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg
    23701 gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt
    23761 ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt
    23821 cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg
    23881 gctttcagga aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt
    23941 cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt
    24001 caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt
    24061 aggaatagta ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa
    24121 acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc
    24181 ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg
    24241 ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta
    24301 ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct
    24361 tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt
    24421 ttagcaatcc aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact
    24481 ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct
    24541 gttttagatg cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg
    24601 caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg
    24661 tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg
    24721 tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa
    24781 tatacgatgg ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg
    24841 actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa
    24901 cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg
    24961 caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta
    25021 gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact
    25081 gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa
    25141 aaagatcagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag
    25201 ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat
    25261 gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc
    25321 gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa
    25381 gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa
    25441 atcaaagaat tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca
    25501 gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag
    25561 tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa
    25621 aaaatgtcag aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt
    25681 tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt
    25741 gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat
    25801 atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt
    25861 gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa
    25921 cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg
    25981 cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt
    26041 ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg
    26101 aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac
    26161 ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt
    26221 acagtgacat ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta
    26281 cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat
    26341 gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga
    26401 agtatcaaca gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac
    26461 caaatgttgc aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg
    26521 acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt
    26581 cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg
    26641 ccttattgcc gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa
    26701 ttatcgccga gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt
    26761 aatttgtgac cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat
    26821 tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat
    26881 ctaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg
    26941 atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa
    27001 ttattttata cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact
    27061 atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa
    27121 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
    27181 gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc
    27241 tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt
    27301 gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac
    27361 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt
    27421 gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat
    27481 gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc
    27541 gaactatcgg cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa
    27601 ttaaataaaa acgttttttt taattggtag tacttttggc tagacaatga taaaatgata
    27661 gagaacactc taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat
    27721 tagtgaaaaa attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca
    27781 tcgtaactgg ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt
    27841 cccataatcg tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc
    27901 cagaagagcg tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta
    27961 tttcaagagg attaccagct ccagaagaaa tgatccaggc agcaaataaa aatgggatag
    28021 ctgtactgcg ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag
    28081 atggtcgcct tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag
    28141 gtgtactgat tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca
    28201 aacgcggcca tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa
    28261 cggtgatcgg agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga
    28321 tcattgatgt aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt
    28381 tggctgttta tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag
    28441 acacgaccgt tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag
    28501 gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg
    28561 gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag
    28621 gcaacgacta gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct
    28681 tgtagggatt cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat
    28741 gtggcttagc agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt
    28801 tatgttgtgg ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga
    28861 tcttccccag tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc
    28921 tatttatggc ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt
    28981 tatttctttg tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat
    29041 cggtcgttgg ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt
    29101 cttagaaagt ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta
    29161 tcgtcaacca acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt
    29221 gtttttacga aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat
    29281 gtggtatagt ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt
    29341 aggtgtgatc cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct
    29401 atttatttgg cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa
    29461 tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa
    29521 tgaattatag agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc
    29581 tcgccgaaaa cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa
    29641 tcaacaccca acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc
    29701 aagcattcac caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc
    29761 caacgaaagc aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag
    29821 tgatcatcca tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct
    29881 tgttagagga aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc
    29941 gtgcagaaga agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa
    30001 aagcagcaaa atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc
    30061 ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg
    30121 gcgctattca cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat
    30181 tagctgagat cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat
    30241 taagcggaat aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg
    30301 caggcaactt gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg
    30361 tcgttgaagg tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg
    30421 aaatgcccat cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag
    30481 ccgcaaaaga aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt
    30541 tttgaaggag cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg
    30601 aacaagattt ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa
    30661 accaacaatc cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat
    30721 cgttacagga aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga
    30781 aacaaaccta agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga
    30841 tgtcaacttg catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca
    30901 agcaagagcc tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga
    30961 agacgaagta ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac
    31021 gatcgccgtt atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg
    31081 agaagtattg gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag
    31141 caaagcacca agtgatttag ctattattgg acgttactta ttgactccag aaatttttga
    31201 tgtattgtcc gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga
    31261 tacattgaat aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt
    31321 cggtgacaaa tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt
    31381 aggtgcacct ttacgtgagt acatcctgtc tttaggtaaa acattagaag cagaaggaag
    31441 caagatcgat cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc
    31501 cttttttcta acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat
    31561 ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga
    31621 tcatgtcagg aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg
    31681 tttttatcgt tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg
    31741 tagcagaagc aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttatctagac
    31801 aagtagaagg cttactggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg
    31861 caacgatcga tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta
    31921 atcattctag taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag
    31981 cgacggttgc cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata
    32041 ctgaaacgac aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt
    32101 agtcggaggc acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga
    32161 acttcgggat gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac
    32221 agatatggcg aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga
    32281 tctttctcaa caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc
    32341 agatgaagca ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga
    32401 cttatctaat cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg
    32461 aagtattgct aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa
    32521 agcaggagat actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa
    32581 agaaatgatg aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac
    32641 aatgacagat tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag
    32701 cggactagct ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta
    32761 gtcgtgaatg gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct
    32821 actttttcag gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact
    32881 aagtcatttc cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct
    32941 aaacggtgat tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga
    33001 tcaagttcac ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca
    33061 agagtgatgt tgtagatttt ttcttggaag tcggttggtt tttgataagc aattgtacga
    33121 gtagcatcac tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc
    33181 ggtgaaatct tcgtatttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg
    33241 actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcaatttct
    33301 gcaatgatcg cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca
    33361 acatcggccc atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt
    33421 tgtagacgtt ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca
    33481 gagatagcat cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc
    33541 tgaccatagc gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa
    33601 tagccacgga catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga
    33661 gtgaaaagga aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga
    33721 tcactttcgt atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg
    33781 acttggtgtt gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa
    33841 cctactttct aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa
    33901 cgttctaaaa actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat
    33961 tcttgagatt atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata
    34021 aaatatgaat aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag
    34081 agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa
    34141 accagcaaca agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc
    34201 agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt
    34261 cagcaatatg ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa
    34321 atacaatatt atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa
    34381 taacttactg gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga
    34441 aatccgtgga gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc
    34501 agatgaacaa gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa
    34561 caaattggca aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc
    34621 catcaatgga caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc
    34681 atatagcgaa ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt
    34741 cgatcggatc cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat
    34801 cggtattttg gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat
    34861 cacaagtaac aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca
    34921 accattatac gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga
    34981 agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa
    35041 ataataaaag gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg
    35101 agcaatttca aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt
    35161 ggcaggagaa caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt
    35221 tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa
    35281 tccgtgtata gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat
    35341 actattgtca caatccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg
    35401 attctttagt tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat
    35461 agttttcatc ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg
    35521 cccatagcga ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt
    35581 ttccaggtgt ttgaccagta aatccagata cttttttctt catcacatca gatgataatt
    35641 cgaatttctc atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat
    35701 tggcaagata attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt
    35761 gccctgccca gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt
    35821 attcattggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
    35881 ttgagatggc atctttgaat ggtgtagtga tttttgcatt gatcacacta cgcatcatgt
    35941 cattcatgat cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat
    36001 aaattacatt tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc
    36061 cgccgtttgc taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac
    36121 ctaaaggagc agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt
    36181 agtgctcgta agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat
    36241 aagccgggat attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc
    36301 cttcgttagt agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg
    36361 cataatctga tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa
    36421 caagaactgg tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt
    36481 gattctgact gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca
    36541 tcaatacatt tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat
    36601 tttcaacgcc attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat
    36661 atcctttatt ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat
    36721 cagacatatc agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat
    36781 tcatcacagt ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa
    36841 agtcttttgt aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt
    36901 cacgatacat actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt
    36961 ttagcgcgcc tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga
    37021 tataagcagc ttgcggcagc gtcacatctt ttgcatctac accaaagatt ccttttgcag
    37081 cttcctggac tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca
    37141 aatacgtagc aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga
    37201 tctcgtttgc tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt
    37261 gctgtgtcaa agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc
    37321 gtaaaacagc tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga
    37381 taattgcagt tttcaataaa ggtgaaatct tatcagatga aatagtcgtg cgcatcagat
    37441 ctgtctggat ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa
    37501 tgtttcctag ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat
    37561 aggcaaaata gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag
    37621 taataaaaac agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt
    37681 ctttttttct tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa
    37741 ctaatggttt tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt
    37801 ttttggcagg aatttctttt ttaaaagagt taaaacgtga gaaaatgatc tttattataa
    37861 aagagttctt aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga
    37921 aaaatcaatc attttctttg tatatcatct taccaatata taggaattgt tagtaacaat
    37981 tgcctttcaa ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag
    38041 aaaatgctgt taggagcagc agcagaagca gccagttatg gatcttcgac ttttatgatc
    38101 tatacaggcg cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc
    38161 ggaaaagcgt acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc
    38221 attaatttgg ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga
    38281 gaagagattg ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct
    38341 catgtagggg caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt
    38401 ttgacaaaag aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa
    38461 atcggtcgaa catttgagga attagcgcaa atcatcgatg gagtaaaatt aaatgaaaaa
    38521 ttatccgtta cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac
    38581 ttttcaggtg taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc
    38641 catatcaatg attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt
    38701 tttggaacaa tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat
    38761 atttcgaaaa tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca
    38821 tatggaaaag aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag
    38881 ttattagcag aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca
    38941 ggcacattgc cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt
    39001 catcatgcat cttctccccc ctctttttaa atttagacaa acaaggtata ctgttagaaa
    39061 agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga
    39121 tgctgaacac tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc
    39181 ttggcatagc cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg
    39241 gaacagacat gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt
    39301 tggacggtaa atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca
    39361 gcttattttt agtacttgca cataaactgc atctttctta tgctgttttg ttcggcatgt
    39421 tcagtatttt tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt
    39481 atcttctttt aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat
    39541 attccactcg aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt
    39601 gtttactagc tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt
    39661 tagggctttc tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc
    39721 ttccttttac tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact
    39781 tttctttcaa agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat
    39841 ggctgaaact ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcagtgga
    39901 tcgaatataa aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag
    39961 aatttggacg tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag
    40021 aaaacagtga gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta
    40081 cattccatac tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag
    40141 gtggaaaagt aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa
    40201 gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca
    40261 accaagaatt agaagaagcc tatcacaagg gaaccatcaa aaagaaaaaa gagttctatc
    40321 tgatgggatc aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc
    40381 aatcaggata cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt
    40441 tcggaacgca agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga
    40501 attggatcga tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag
    40561 tcgcgaatgt catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa
    40621 tagcagggat attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt
    40681 atagttcctc tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg
    40741 gtgtctcgtg gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca
    40801 ctggcgcagg cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta
    40861 aattaaatct gaaaaaggct ttatcttcta aataaaaaga cccttggtag ctagattttt
    40921 aacctaacta tcaagggtct ttgtgtggaa aaatcagttt ctattcgatt tcttctaccg
    40981 tttcctgcaa acgctgttca tattttttgt ttcctttata taaatcacca atcgtccatg
    41041 ccaacaggag aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt
    41101 gagcagaagt ggcttgttca ccaaagaaac tttcgatctg tcctggtaat cctcgtaagt
    41161 tcaagtaagt tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt
    41221 taaatcggct acccatctcc gcttggctgt tggtcatcat cagtaaaggg atcatcgaaa
    41281 acggcaacgc aaaagccaag aacacctggg attcattcat caagttattc aaagcggtat
    41341 gttcttcgat cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag
    41401 aaagcaatcg tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaagcctt
    41461 ccatgatgac ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa
    41521 gtgctacagc aaataaagtg gataagatac cagattttgc tacagcaatc aagataccat
    41581 tgctcatagc tgatggatca gataaggctt catacagacc aaaaaaggaa ggatcttgga
    41641 cagaaccggt tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag
    41701 ccatcgacaa ttggatatta gagtcccaag tagaaaaacg tacagcttgt gctacatctt
    41761 cttcgtcttg gtgatcaatc ttccgagttt gagaaactgc tgaatgaaga tataagttat
    41821 gcggcatgac tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga
    41881 tgaccggtga gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc
    41941 aatctgggtt agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc
    42001 agacaaccag tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa
    42061 catctaatac agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg
    42121 cgattgctgc accaatgact tctgcgatgt ctgttgccat aatggctaat tcagtcaaga
    42181 tccataaaat gacacccaat gtcttacttg tgcgtgctcg gattgcttgg gccaaatcca
    42241 tttgggtcac gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag
    42301 acatcaaaat aactgacatc aataaatatt ggaaattttg tccaccagtg atcgaagtgg
    42361 accagttacc tggatccata taaccaactg ctactaatgc acctggtcca gagtaagcaa
    42421 gaagtgtttt aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta
    42481 aagataaacc atttgtatgt tcaataagtc gatgcttatg aaattgtttg ttggattgat
    42541 tattcaagaa aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga
    42601 aaaaattaaa gaaaaatatt cggtatgcct aatttatttt cgtggcgcgt cttgttagat
    42661 gacgaaagaa attttgttct cctctaaaaa aaccactatt ttttgcatga aactaaagaa
    42721 tacgcctttc ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat
    42781 agaaaggaga gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt
    42841 tgctaatcgg cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag
    42901 aaaaaaatcc tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atgggacaaa
    42961 aaccatctga gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat
    43021 aggttctgac ccaacgactt tttacgggct gattttttca gtccgttttt tattttgtaa
    43081 tttgttagac tattcagatt attcagcaaa aagacaagca cagacaatcc taggctcttg
    43141 ataaagacaa aggaggagta aaatgtcgat ttttagaaag ttaggctggt tttttcgtca
    43201 ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt
    43261 agttcctcca aaagtgattg ggatcatcat tgatgagatt gccgataaaa atttatcagt
    43321 aaagatcatt ttcgggtgga tcttcctatt gattatcgct gctttcttac aatatctttt
    43381 tcgttatatc tggcggacga atatttgggg aagtgcagct cggttggaga aagaacttcg
    43441 gcagcagtta ttcaaacatt ttacaaaaat ggacaatctc ttttaccaaa aatatcggac
    43501 tggggatttg atggctcatg caacgaatga tttgaatgcg atccagaatg tagcaggagc
    43561 tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgtgatgat
    43621 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt
    43681 cacttcacga gtgctggggt cgaaactcca tgatgctttt cgtgattcgc aagaagcttt
    43741 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    43801 tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa
    43861 aaacaaacga gtcaattttc tagactcctt atttgatcca ttcattacct tgattatcgg
    43921 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    43981 gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    44041 gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga
    44101 acttttgcat gaaaaaacgc atatcgtgga acaactgggt gcagtgcaga ccccggtaaa
    44161 aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt
    44221 agaacaaatc aagtttgcaa tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    44281 tgcgggaaaa acaacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    44341 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    44401 cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt
    44461 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    44521 caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc
    44581 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    44641 attattaata ttagatgatg cactttctgc agtagatgcc aaaacagaag aagctatctt
    44701 gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag
    44761 tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    44821 aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca
    44881 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    44941 acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc
    45001 aaacctttta gaacaacgtt catcgttgct atcttatttg cgtttgctct gtctgtgatc
    45061 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg
    45121 gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt cctagtaaaa
    45181 agtatcattt ggtttttcca atggtatttg tattccatgg cttcattgaa gacctatcag
    45241 tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag
    45301 acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    45361 tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc attcattgcc
    45421 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgttcttgcc gattttagga
    45481 attgtcatct ggtattacca gaaatttagt tctcgtattt accgaaatat gcgggaaaaa
    45541 ttaagtcagc tgaatacaaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    45601 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac
    45661 acgcgctatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta
    45721 tatgcattgg cgattgcgtt ggcgttgact ttatttggga tcgatgcgtt gcattctccc
    45781 gtagaagttg gactgatcta tgcatttacg acttatgtcc agggattttt taatccaatg
    45841 tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc
    45901 ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg
    45961 attacttcag gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    46021 gtattaaaca atatctcttt tgttgctaat ccgggagaaa ccgttgcatt agtgggacac
    46081 accggaagtg ggaaaagctc gatcatcaat gtgttgatgc ggttttacga attttatgaa
    46141 ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    46201 aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt
    46261 cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa
    46321 gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt
    46381 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat
    46441 cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg
    46501 atccaagaag gattggcgaa aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg
    46561 ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa
    46621 cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    46681 caaagtagtg gtatataaaa gatgttgtga cagtagcatt ttgacctaag cactagagtg
    46741 aaaataaaac atagcttccc atatttttca tctgtgagag ttagtgtcgt agttaccaga
    46801 tttaaaaaag acgacgtcag ctagtcagaa gtgcctaata gcccctaaga aagcgttgac
    46861 aaaatcgttt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    46921 tgcaagctaa acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt
    46981 ttgttttgga tacagcccta tatatcatgg aaaggagcaa aacatgctga ttgagaaagt
    47041 actgaataac aacgtcgtaa taacaaggga tgaaagagat cgggaaatga ttgtgatggg
    47101 aaaaggattg gcattcaaaa agaaggtggg tgacccgatt cttgactcat gcatcgacaa
    47161 aaaatttcag ttgacaaatg acggactttc acaacagttc caagaattgg cacaagcgat
    47221 tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctgca
    47281 agtccattta aatgattctg tgtatatttc attgacggat cacttgtata cagctatcga
    47341 acgtcaaaaa atggggatct ttgtgccaaa tgtattgctg tatgatatca aacaactgtt
    47401 tcctaaagag tatgccattg ggaaaaaaac agtgaagaaa atctcggata aataccacat
    47461 caagttatca gaagacgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat
    47521 tcaaagccag caagacaata cctacctgtt taccacaaca atacaggata ttttaaatat
    47581 cgtccgctat tactataaga ttacatttga tgaagattca gtccactttt ttcgattcgt
    47641 cacacacctg cgtttttttc tgacacgtgt cctagatgga gcgaaatcag aagatggaga
    47701 agtttctgag gaattgttcc aagttattca aaaaaaatat aaggatacgt ttggttgcgt
    47761 tgagaaaata gcagactttt taaccggggc tttcgactac cagatgtcca cggatgaaaa
    47821 aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaataaa
    47881 aaaggaggac aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa
    47941 ccttccacta attaattgga gggaatagaa atgggaaaat accaggaatt agcagaaaac
    48001 atcgtaaaga atatcggagg aaaagaaaat atccgtagct tgacacactg tgtgacgcga
    48061 cttcgtttca aactaaagga ggaaaataaa gcaaaggatg atgttttgaa aaagatggaa
    48121 gggatagtca ccatcatgaa aagcggcggg caatatcaag ttgtaatcgg gaatcatgtg
    48181 ccagatgtct atgcagaagt actgacagta gccgggattt ccgatgaagc agaagaagag
    48241 gtttcttcaa gtagtaatct gttcaatcga ttaatcgaca tattaagtgg ctgtttccag
    48301 ccttttttag gtgctttggc tgcagcggga atggtcaaag gattaaatgc tttgttcttg
    48361 tttctcaaat tttatactga aggatcgggt acgtacatca tgctgaatgg gataggggat
    48421 gcaatttttt atttcatgcc agtgatctta gggtacacag cagcgaaaaa atttcatctg
    48481 aatccgatgg tggggctcgt gattggtgca gcactttgtt atccgactgt acaaggagca
    48541 gcactacaaa gtggtttccc tgatggcagt cctgcgcctt atagcttgct gggtcttccg
    48601 gcttatgaaa catttttggg tatcccatgg gtgggcagca gttatactag tagcgtcgta
    48661 cctgttatct tcattatcgc atttgccgga caagtccaaa aaattgccaa gaaatggatt
    48721 cctgcagtcg tccagacctt tttggtttct ttttctgtgt tattaatcgc acttcctgct
    48781 ggctttttga ttatcggtcc gattgtcagt atactgacag atctattaag cacgggattc
    48841 caagcattga tggacttttc tccagctgtg tatggtgcag ttctagggtt tctttggcaa
    48901 gtactggtga ttttcggact tcattggagt attatccctc ttgcgattat gcagctagcg
    48961 caaaatgggt ggagtcagat gttagtaggc gtttatggcg cgagttttgc acagacagca
    49021 gtggtattgg cgatgtattt caagttcaag gatcaaaaaa tgaaggcgct ttgcatgcca
    49081 gctgtgattt caggaatctt cggcgtaaca gaaccggcta tttatggaat caccttaccg
    49141 aagaagaagc catttattta ctcgatgatt ggtggtgctg tttcaggaat ctatttgatg
    49201 gcatctggaa caatcagcta cataatgggc ggattaggga tttttggggt ggtaaactac
    49261 atcacggaaa cagacgccag tggtatgttt caagcgttta tcggcattgc tattgcttcg
    49321 gtaatcgggt tttcgctgac ttttttcttt tggaaggatg aagcaggtga aacagatgct
    49381 atacagaaaa aatcaggtaa agaaattgta aaaacaccga tacaaggaca aatcgctcca
    49441 ctaaatgcag cgaaggatgc agcttttgca caaggaacac taggtcgagg aatcttaatc
    49501 taccctgaaa aaggagaagt acgtgcccct tttgacggaa cagttatgac cctgttcccg
    49561 acgaaacatg caatcggaat ggtttctgaa acagggctgg aattgctgat tcatgtcgga
    49621 ttggatactg tgcagttgga agggaaatat tttgaaagct tagtgcagca aggagaaaga
    49681 gtgagacaag gcgatctgtt gctacgcttc gacaaaaaga aaatcgaaaa ggaaggatac
    49741 agtttagaaa cacctgtgat cgtcaccaat tacacggatt atttggatat tttggaaaac
    49801 caaggggaga cggtcgggcc agacgatgca cttatcaccg cactgacctg aacggttagg
    49861 tgaagaatgg actaggctta actattcttt ttattccact tctttatttt caaaattgaa
    49921 aggctctttc atgatggctg agctgatagg gcgtttatcg aatttttgac cgacagaaat
    49981 tagatggact aaattctgtt gataattttc tgcaaaaaca caactatata acacgtctag
    50041 aagataacaa attgatgtat tgatcgtaaa attaccaatt tttgaataca gtcgctcgcg
    50101 tgtcgtaagt cgcaaagcgg ctgtactcat tgcagatagc gttgaatcac cgatactggt
    50161 caaagctaaa attggaacat cattttgttt caatatttct gcggtacgaa ggatcacatt
    50221 gttttcaccc gtataagaaa tcagaatccc gcacatatcg ctcgtacaat tgtacgcttc
    50281 atagaatgat tcaccaaaag tggtgacaat tgtgacatcc attttgatgc gcttcatttt
    50341 cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg gcgaagatca cgattttttt
    50401 tgcctggagc agtaaatatt tagctttttg cagttggtca tgatgaatga gagaaagtgt
    50461 atcatgaatc gtcatctgtt ctaaagaagc cattttattt gcgattgtca taataccgtc
    50521 tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt
    50581 ttcagctaaa aaagcattac gaaaactgac ccagccatca aaacccattt tttttgctac
    50641 acggatgagt gtagacgggt ggacataaca tcgctcagct atcgcttgga tggtcatttc
    50701 gccaagtgct ggagaatcgc ttaagagaaa ctggatcact tcattttctg aaggagaaaa
    50761 ggagatttct ttgatttttt cacgtaataa catgtagata ccttcttctt atttttttac
    50821 gtaaattata acttttttga aaatgttttg taaac
//
LOCUS       DAXWLG010000002        50574 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXWLG010000002 DAXWLG010000000
VERSION     DAXWLG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.002"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE4T_000052"
     CDS             complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE4T_000052"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8385372.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHI"
     gene            complement(1868..2245)
                     /locus_tag="ACGE4T_000053"
     CDS             complement(1868..2245)
                     /locus_tag="ACGE4T_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385373.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            2501..3229
                     /gene="purC"
                     /locus_tag="ACGE4T_000054"
     CDS             2501..3229
                     /gene="purC"
                     /locus_tag="ACGE4T_000054"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFF8385374.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            3229..3483
                     /gene="purS"
                     /locus_tag="ACGE4T_000055"
     CDS             3229..3483
                     /gene="purS"
                     /locus_tag="ACGE4T_000055"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFF8385375.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            3485..4156
                     /gene="purQ"
                     /locus_tag="ACGE4T_000056"
     CDS             3485..4156
                     /gene="purQ"
                     /locus_tag="ACGE4T_000056"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFF8385376.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            4157..6379
                     /gene="purL"
                     /locus_tag="ACGE4T_000057"
     CDS             4157..6379
                     /gene="purL"
                     /locus_tag="ACGE4T_000057"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFF8385377.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            6364..7803
                     /gene="purF"
                     /locus_tag="ACGE4T_000058"
     CDS             6364..7803
                     /gene="purF"
                     /locus_tag="ACGE4T_000058"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFF8385378.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            7826..8878
                     /gene="purM"
                     /locus_tag="ACGE4T_000059"
     CDS             7826..8878
                     /gene="purM"
                     /locus_tag="ACGE4T_000059"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFF8385379.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            8875..9453
                     /gene="purN"
                     /locus_tag="ACGE4T_000060"
     CDS             8875..9453
                     /gene="purN"
                     /locus_tag="ACGE4T_000060"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFF8385380.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            9450..10985
                     /gene="purH"
                     /locus_tag="ACGE4T_000061"
     CDS             9450..10985
                     /gene="purH"
                     /locus_tag="ACGE4T_000061"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFF8385381.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            11001..12275
                     /gene="purD"
                     /locus_tag="ACGE4T_000062"
     CDS             11001..12275
                     /gene="purD"
                     /locus_tag="ACGE4T_000062"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFF8385382.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            12397..13311
                     /locus_tag="ACGE4T_000063"
     CDS             12397..13311
                     /locus_tag="ACGE4T_000063"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFF8385383.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(13444..15009)
                     /locus_tag="ACGE4T_000064"
     CDS             complement(13444..15009)
                     /locus_tag="ACGE4T_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385384.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(15002..16453)
                     /locus_tag="ACGE4T_000065"
     CDS             complement(15002..16453)
                     /locus_tag="ACGE4T_000065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8385385.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(16467..17483)
                     /locus_tag="ACGE4T_000066"
     CDS             complement(16467..17483)
                     /locus_tag="ACGE4T_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8385386.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            17703..18791
                     /gene="chvE"
                     /locus_tag="ACGE4T_000067"
     CDS             17703..18791
                     /gene="chvE"
                     /locus_tag="ACGE4T_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8385387.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            18845..20371
                     /gene="mmsA"
                     /locus_tag="ACGE4T_000068"
     CDS             18845..20371
                     /gene="mmsA"
                     /locus_tag="ACGE4T_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8385388.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            20373..21578
                     /gene="mmsB"
                     /locus_tag="ACGE4T_000069"
     CDS             20373..21578
                     /gene="mmsB"
                     /locus_tag="ACGE4T_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFF8385389.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAVRLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            21835..22812
                     /gene="guaC"
                     /locus_tag="ACGE4T_000070"
     CDS             21835..22812
                     /gene="guaC"
                     /locus_tag="ACGE4T_000070"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFF8385390.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            23076..24500
                     /locus_tag="ACGE4T_000071"
     CDS             23076..24500
                     /locus_tag="ACGE4T_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8385391.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(24542..24799)
                     /locus_tag="ACGE4T_000072"
     CDS             complement(24542..24799)
                     /locus_tag="ACGE4T_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385392.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            24961..26070
                     /gene="pdhA"
                     /locus_tag="ACGE4T_000073"
     CDS             24961..26070
                     /gene="pdhA"
                     /locus_tag="ACGE4T_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFF8385393.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            26073..27050
                     /locus_tag="ACGE4T_000074"
     CDS             26073..27050
                     /locus_tag="ACGE4T_000074"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFF8385394.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            27075..28718
                     /locus_tag="ACGE4T_000075"
     CDS             27075..28718
                     /locus_tag="ACGE4T_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFF8385395.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEIQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            28726..30132
                     /gene="lpdA"
                     /locus_tag="ACGE4T_000076"
     CDS             28726..30132
                     /gene="lpdA"
                     /locus_tag="ACGE4T_000076"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFF8385396.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            30294..30569
                     /locus_tag="ACGE4T_000077"
     CDS             30294..30569
                     /locus_tag="ACGE4T_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFF8385397.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            30575..31342
                     /locus_tag="ACGE4T_000078"
     CDS             30575..31342
                     /locus_tag="ACGE4T_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFF8385398.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            31510..33339
                     /gene="typA"
                     /locus_tag="ACGE4T_000079"
     CDS             31510..33339
                     /gene="typA"
                     /locus_tag="ACGE4T_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFF8385399.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            33600..35372
                     /gene="recQ"
                     /locus_tag="ACGE4T_000080"
     CDS             33600..35372
                     /gene="recQ"
                     /locus_tag="ACGE4T_000080"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFF8385400.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            35486..35788
                     /locus_tag="ACGE4T_000081"
     CDS             35486..35788
                     /locus_tag="ACGE4T_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFF8385401.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            35791..36954
                     /locus_tag="ACGE4T_000082"
     CDS             35791..36954
                     /locus_tag="ACGE4T_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8385402.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            36980..40408
                     /locus_tag="ACGE4T_000083"
     CDS             36980..40408
                     /locus_tag="ACGE4T_000083"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFF8385403.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            40429..41553
                     /locus_tag="ACGE4T_000084"
     CDS             40429..41553
                     /locus_tag="ACGE4T_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFF8385404.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            41534..41980
                     /locus_tag="ACGE4T_000085"
     CDS             41534..41980
                     /locus_tag="ACGE4T_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8385405.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            41964..42302
                     /locus_tag="ACGE4T_000086"
     CDS             41964..42302
                     /locus_tag="ACGE4T_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFF8385406.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            42421..42981
                     /gene="rsmD"
                     /locus_tag="ACGE4T_000087"
     CDS             42421..42981
                     /gene="rsmD"
                     /locus_tag="ACGE4T_000087"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFF8385407.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTIDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            42978..43469
                     /gene="coaD"
                     /locus_tag="ACGE4T_000088"
     CDS             42978..43469
                     /gene="coaD"
                     /locus_tag="ACGE4T_000088"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFF8385408.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            43459..44502
                     /locus_tag="ACGE4T_000089"
     CDS             43459..44502
                     /locus_tag="ACGE4T_000089"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFF8385409.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            44661..45389
                     /locus_tag="ACGE4T_000090"
     CDS             44661..45389
                     /locus_tag="ACGE4T_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFF8385410.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            45505..45750
                     /locus_tag="ACGE4T_000091"
     CDS             45505..45750
                     /locus_tag="ACGE4T_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8385411.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            45747..45965
                     /locus_tag="ACGE4T_000092"
     CDS             45747..45965
                     /locus_tag="ACGE4T_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFF8385412.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            46101..46640
                     /locus_tag="ACGE4T_000093"
     CDS             46101..46640
                     /locus_tag="ACGE4T_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFF8385413.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            46633..48906
                     /locus_tag="ACGE4T_000094"
     CDS             46633..48906
                     /locus_tag="ACGE4T_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFF8385414.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            48944..49990
                     /gene="holA"
                     /locus_tag="ACGE4T_000095"
     CDS             48944..49990
                     /gene="holA"
                     /locus_tag="ACGE4T_000095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFF8385415.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(50079..50333)
                     /gene="rpsT"
                     /locus_tag="ACGE4T_000096"
     CDS             complement(50079..50333)
                     /gene="rpsT"
                     /locus_tag="ACGE4T_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFF8385416.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
BASE COUNT        16469 a         8736 c        11101 g        14268 t
ORIGIN      
        1 aatatgactt ttggagactg accaaatacc ttttcttgta tttgaccatt catacgcttt
       61 cgctttatcg atccctaaac gaatcagatt ttttcttttg gttcttggat tcttccattg
      121 tttccaaata tattgtctga ttcttcgctt caaccaaccg tttaactccc tcatgaattc
      181 tttcatctca ccaattccat agtagttgat ccaaccagtc attagttgct tgatttcttt
      241 taaaataagt tggatacttc ttccacggtt tcttttagtg agttgtttga gcttgctttt
      301 cacacgctgt tttgcttttg cgtgagggcg aattttaata cctttctttg ttggcagaat
      361 gcagaatcca agaaactttc gtttcaatgg acttcctacc gcactctttt ctcgatttac
      421 cgtcaacttt agttgttctt cgagaaattg aatggatttc ttcatcacac gctgtcctgc
      481 tcgtttgctt ctaacataga tattgcagtc atcggcgtat cgaacaaatt tatgtcctag
      541 attttctagc acttgatcaa attcgttcag gtagatgtta cttaacaggg gagagagatt
      601 gcctccttgg ggcgttcctt cttcagttgt tgtgatgagt ccattttcca ttacaccact
      661 cattaagtat tgacggatca gttttaggac ttgtttgtcc tttactttcc gctcaatgaa
      721 atacatcagc ttatcgtgat ttacggtgtc aaagtaggct ttcatatcaa tgtccaccac
      781 atatcggtaa ccttgttcat aataagattt tgctttcttc actgcgtcat gtgcgctgcg
      841 atttgggcga aacccataac tattatctga gaattctggt tcaaatagcg gttgtaacac
      901 ttgattgatt gcctgttgaa gcatacgatc aataaccgtg ggaattccta gttttcttac
      961 accgccgttt ggtttcggta tttctactcg caaaacaggc tgaggtttat agttcccttg
     1021 gcgtaaattg gatagtaatt cctcgcggtg ctctttcaag taaggaagca gttgatcgac
     1081 ggtcatccca tctgcaccag ccgccccttt atttctgact acttgaagat acgcttgatt
     1141 gagattattc cgatctagaa ttctcccaaa cagattttct acaccatctt gtttcctctg
     1201 ttcaccgcaa gaagtgctgc gcacttcatc atacccttcg tgttccacac tagctctttg
     1261 atgatagcca agttttcttg ttttctgcgg tcgtcgcatc ttctcacacc tcctaaatta
     1321 ttcaagattc ttattgttca gtccttcgta acggtcagtg tcgttactac tatgacctcg
     1381 gctgacttct catgattcac catcatgtcg ccatgatcgt tgtgctggtt tgttttcatt
     1441 caacgcccac aacaccctca tgagatctcc cccggtaaga gtgaaaactt tcctcccatg
     1501 taactgctag atttactgta taggtttcgg gcagtattgg actttgtctt gtgatgaaga
     1561 cttatccgac ctaattcagc cttgtatcta gtttctgttc gtcagttcag gagtttgcgt
     1621 ccgacttcct tcagccacaa cctcacgatt gccgccttgt ctttcgctaa cagttcctac
     1681 tgccaagcct gtagtggact ttcaccacca agttttcacc catgcagggc gcacaccaaa
     1741 aaagaagcag atccgttagc ggttctgctt cttttagttt attatatttt cttatcaata
     1801 ataggaacct gattttaaaa gcggctacct ctttaccgtg cctctttaca tacaaaaaat
     1861 agaggaatta agcgtaaatg gcttggctat tataactaaa atgctggtta tggagatgtt
     1921 gcagcaagtt ttcgagaaaa gcgtattcca tctcttgtcc attgaataag atctggatac
     1981 gataatgatt tttgaaacgc ttctgttcat gaatctgact gattgaaagt tcgcctttgt
     2041 gttctgtctg ataaagaaaa gtccttttac ggcattcagc acagcgcttg gttgctcggc
     2101 ggatttttcg gaatgtataa aaactagtat ctttattgca taaataaacg tctttgatcg
     2161 acagcaaatc attatataga caggaacgtt cttcagctgt taattctgat aaaaaaagct
     2221 gaccttgttt tattgcatct tccatcggca agtgcctcct cgaacccatt atccaaattt
     2281 ttagttaatt ctaatatacc atcattaagt ataaatgtaa acgcttgttg atggtataag
     2341 attttcgttt ctgataaaac acgaacatta aaagaatcta atgccttaaa tgtacatatt
     2401 tttgttgatt tataaaaaga tgattgctaa aatttaaagt gaaaaaacga acaaaaaacg
     2461 atgtatttgc ttttaatgta aataaaaagg agagacgtac atggagaaga aagaactttt
     2521 gtatgaagga aaagccaaaa aaatatatct tacttctgat gagaacttga tttggataga
     2581 atatttagat caagcaactg cgttgaatgg cgcgaggaaa gaccagataa gtggaaaagg
     2641 agaattgaat aatcaaatca caagtgcaat tttccgttat ctgactcaaa gaggaataaa
     2701 aaatcatttt attgaacaat tgtctaaaac cgaacaatta gcaaaaaaag tacaaattat
     2761 ccctctggaa gtggttatcc gaaatattgc agcaggaagt ttctcaaaac gtctaggtgt
     2821 tcccgaaggt aaagaattca tcaaaccaat cattgaattc tactataagg atgaccagtt
     2881 ggatgatcca tttatcaatg aagagcatat ctatttctta caacttgcaa agccggaaga
     2941 aatcagtgag ctgaaagccc aagcgttggc tattaatgaa attttgaccg aattatttgc
     3001 taaggtcggt ttggttttag tagatttcaa gctagaattt ggccggtcga atggacaaat
     3061 tttgcttgcg gatgaagtaa caccggatac ttgccgatta tgggatgaac gtacgaatga
     3121 acatatggac aaagacgttt accgcagaga tctaggtgat ttgatcccag tctatcaaga
     3181 agtatataac cgtctatcgg aactaactga agaaaaggag cgagtatgat gtatttcgta
     3241 aaagtatatg taacctataa agattcagtg ttagatcctc aaggggaggc agtaaaaaaa
     3301 gcagtccatc gtatgggata tgaaacaatc gaagatatcc gaataggaaa atactttgag
     3361 atacaacttg ctgatgcaga agaaccaatc gatacagtga ttgaaacgat ttgtgacaag
     3421 ctgcttgcaa atgtaaatat ggaaacatac cgatatgaaa tcaaaaaaat ggaggaagta
     3481 taacatgaaa tttgcggtca tcgtttttcc aggttcaaat tgtgacgcag atatgttatg
     3541 ggcagttcgc gagatcatgg gcgctgatgc cgaatttgta cggcatgatg agtcctcttt
     3601 agaaggattt gatggggttt tactgccggg aggattctcc tatggtgact atttgagatg
     3661 cggtgcgatt gctcggtttt ctacaatcat ggaagaagtg attcgttttg ctgaagaagg
     3721 gaaaccagta ttcggtacat gcaatggatt ccagatattg acagaagcag gacttttgcc
     3781 aggtgtactg agacgaaatg agtctttgca ttttatttgc aaaactgtac cattgaaagt
     3841 caaccccaag acggccttta cttcagagta tgaagccgac gaaataatct atcttccagt
     3901 tgctcatggg gaaggaaatt atttctgtga cgaagaaaca cttcaagaac taaaaaaaaa
     3961 taatcagatt gcctttactt ataatgacaa catcaacggc agcgtggaaa acattgcagg
     4021 gattacgaac aaagcaggga atgtgttagg aatgatgcct catccagaac gtgcagtaga
     4081 agacttgcta ggatcagaag atgggaaacg gttctttgct tccattttaa aaaacttcgg
     4141 aaaggtgacc gtatagatga aaacattgaa agaaccgaca gctgaagaga tcaagaaaaa
     4201 aagaatctat gccgaatggg gattgactga tgaagaatat cgaatgatcg aagaagatat
     4261 cttaggaaga atgcctaatt atacggaaac tggactgttt tctgttatgt ggagcgaaca
     4321 ttgttcctat aaaaattcta aaccggttct acgcaaattt cctactagtg gacctcatgt
     4381 gttgcaaggc cctggagaag gcgcaggcat cgtcgacatc ggtgatggac tagcagttgt
     4441 cttcaaagca gaaagtcaca atcatccttc agccgtggag ccttatgaag gagctgcgac
     4501 aggagttggc ggaatcatca gagatatttt cagcatgggg gctcgtccaa ttgcgttgct
     4561 agattcattg aggttcggcg aattagataa tccaagaacg aaatatttac tagaagaagt
     4621 agttgcaggt atcggcggat acggcaattg tatcggcata ccgactgtag gcggcgaaat
     4681 tgcatttgat ccttgttatg aaggaaaccc attagtcaat gcaatgtgcg taggtttgat
     4741 tgaacacaaa catatccaga aagggcaagc cgctggcgtc ggtaattcga tcatgtatgt
     4801 aggtgcaaaa acaggtcgcg acggtatcca cggagcaact tttgcctcag aagaatttaa
     4861 tcaagaagaa gaacagcaac gttctgctgt acaagtaggc gatcctttta tggaaaagct
     4921 gctgcttgaa gcgtgtttgg aattaatctt gcatcattcg gattctctaa taggtataca
     4981 ggatatggga gcagcaggac ttgtttcatc tagtgcggaa atggcttcaa aagctggctc
     5041 aggacttata ttgacattag atgaagtccc acaaagagaa actgggatga caccttatga
     5101 aatgatgtta tcggaatcac aagaacggat gttgatttgt gttaaatcag gagaggaaga
     5161 aaaaatccaa gaactatttc agaagtatga tctagatgca gtcactatcg gtaaagtaac
     5221 agacgacggg caatatcggc tatatcatca aggagaagaa gtagcaaatc tgccagtcga
     5281 tgcattagct gaagatgcac cagtttatca taaagaaatg aaagaaccag ctcgaatcgt
     5341 agaatttcag cagttagctc cttatcagcc agttatcgaa gaacctggac aagtattgct
     5401 tgatctgctg cagcaaccga cgattgcttc aaaacgttca atctacgaaa cttatgattc
     5461 acaagtacaa accaatacag ttgtccgtcc aggaagcgat gctgctgtga tgagggttag
     5521 agggacgaat aaagcgttag ctatgaccac agactgcaac gcgcgctatc tgtatttgga
     5581 tccaaaaatc ggaggacaaa tagcagtggc agaagctgct agaaatatta ttgctagcgg
     5641 tggtaagccc ttggcgatca ccgactgcct gaactatggt tctcctgata aaccagaagt
     5701 tttttgggaa ttatcgactt cagctgacgg aattgctgct gcgtgtgaaa cgttaggaac
     5761 tcctgttatt tcaggaaatg tctctttata taatgaaacg gatggtcaag caatctatcc
     5821 aacaccaatg attggtatgg tcggattgat tgaggatcat aaacatatca ctactcagga
     5881 attcaaaaaa tcaggggatc taatctatat attaggtaag acgtttgcag attttgatgg
     5941 cagtgaatta caaaagatgc agcttggaag aatcgaaggt gtgatccgaa actttgatct
     6001 atccattgaa aagagaaacc aagagttagt attgacagcc atccaaaacg gattgattga
     6061 aagtgctcat gactgttcag aaggtggctt ggcaattgca ctggcagaat cagctttcaa
     6121 acatcagcta gggatatctg tacagtttga attgtcctct gctcaattat ttgcagaaac
     6181 tcaatcaaga tttgtgttga cagtcgcacc ggaaaataag acacgattcg aagaaatgat
     6241 gggagatgct gctgttttag caggtaaggt gacggatgaa gcgataatcg aaatttcagc
     6301 aactgacggt cagatcaaaa tagaaactgc agtagcaaga aagtgctggg aggatgcaat
     6361 cgtatgtctt atgaagtgaa gagtttaaat gaagaatgcg ggatcttcgg tgtttggggt
     6421 catccagacg ccgctagtgt ggcatacttc gggctccata gtcttcagca tcgaggacaa
     6481 gaaggtgcag gtatcgtgtc caatgatcga ggaaagctta aggggcatcg agatttgggg
     6541 ctgctttctg aggtattcaa agatcaaaga aaattgcagc agttaacagg agaagctgca
     6601 atcggacatg tacgttatgc aacagcaggc aatggaagtg tggacaatat tcagcctttt
     6661 ctattcaaat ttcatgatca gtcgatcggc ttagcgcata atggtaactt gatcaatgct
     6721 cgttccctta gaaaagagtt agaggaaaat ggggcgatct tccattctaa ctccgatacg
     6781 gagatattga tgcatttgat ccgacgaagt gaagaaacag attttttaaa taaattaaaa
     6841 agtgctttga atgaagtcaa aggtggcttt gcctatttat taatgacaga aacggcaatg
     6901 attgccgctt tggacccaaa tggttttcgt ccattagcaa tcggacaaat gaaaaacgga
     6961 gcatacgtga ttgcctcgga aacttgtgca ttagaagtta ttggggcacg ttttataaga
     7021 gatgtccagc ctggtgagat agtgatcatc aatgacgaag gaatccagat tgattgcttt
     7081 acgaaggaca cgcagctttc tatttgttcg atggaatata tctattttgc cagaccagat
     7141 tcaaacatcg cagggatcaa tgtgcatact gcacggaaaa atatgggacg gaatttagcg
     7201 aaagaatcgc cagtaaaagc agatatggtc gttggcgtac ctaattcttc tttgtcagcc
     7261 gcaagcggtt atgcggaagc tagtggaatc ccttatgaaa tcggattagt aaaaaatcaa
     7321 tacattgccc gaacatttat ccagccaacg cctgagctaa gagagcaagg tgttcgaatg
     7381 aaattatctg ctgtacgagg agtagtggaa ggtaaaaagg tgattatggt cgatgattca
     7441 attgttcggg gtacgacaag cagacggatc gtccatcttt tgaaagaagc aggggcaaaa
     7501 gaagtccatg tgaggattgc ttctcctcct ttgaaatatc catgttttta cgggattgat
     7561 attcaaacgc gtaaagaact cattgcggca aatcacacga ttgatgaaat caaagaatgt
     7621 atcggtgcag attctttagg ttttttaagt gaagagggtc tgatcgaatc gatcggtctg
     7681 gaaaaagatg ctccgtattc aggattgtgt atggcttatt tcaacggaga ttatccgacg
     7741 ccgttatatg actatgaaga aaaatatcag gaatcactaa aagaaaaagt atcgttcttt
     7801 taaaagcaga ccaagaaggg agtatatgaa ttcaatggca aatgcatacg caaaagctgg
     7861 cgtagatgtc gaagctggat atgaagtagt agaacggatc aagaagcatg tcaaaaaaac
     7921 ggaacgttta ggtatgatgg gcgctatagg aggattcggc ggttgttttg atttatcggt
     7981 ctttcaatta aaagagccag tccttgtctc ggggaccgat ggcgtgggga cgaaattagc
     8041 agtggcaatc gaagcagaca aacacgatac aatcgggatc gactgcgtag cgatgtgtgt
     8101 caatgatatc ttagcacaag gcgcagagcc gttatatttc ttagactact tagcaacggg
     8161 gaaaaatgat ccgttacgtt tggaaaatgt agtaaaaggt gtagcagatg gatgtgttca
     8221 aggaaatatg gcgctgattg gcggagaaac agctgaaatg cctggtatgt actcaggtaa
     8281 tgattatgat ctagccggat ttgctgtagg gatagctgaa aaatctcaat tattgacagg
     8341 agaaacgatc aacccaggag atgtgttgct tggaatagca tctagcggga ttcattcaaa
     8401 tgggtactct cttgttcgca aaattttctt tgagcagcag cagttttctt tatcatcaaa
     8461 actgccagaa ttgaaaaaaa cacttggcga agaattgctt gagccaacga aaatctatgt
     8521 ttcttccttg cttccgctga tcaaagaaaa gaaaataaaa ggagctgcac atatcactgg
     8581 tggagggttt gtagaaaata tcccgcgaat gttaccagaa tctatggctg cacaaatcaa
     8641 acttggctca tggccgattc ttccaatatt cggctgttta caaaaatatg gtgaaattcc
     8701 tgagatggag atgtatgaaa tcttcaatat gggcatcggt atggtcctgg cagtccaaga
     8761 atcgaaagta aaagaaatag tacacgaaat tgagcaagct ggcgaaaaag tttatgaaat
     8821 cgggaaagtg atcgccaaag aaaaacatcg tgtggtgttt ataggagaag aacgatgaga
     8881 atcgctgttt ttgcttctgg aaacggaagc aactttcaag cgctagccga ttatctatcg
     8941 aaaaaaggat tggaatcttc gattgactgg ttgttttgtg atcagcctga agcatatgtt
     9001 ttgaaacgtg caactgcgtt gagtgtaccg gcagattgtt tttcacctaa agaatttgat
     9061 tcaaaaaaag agtatgaaga agcaattttg cataaattga aagaaaaaaa gatcgatttg
     9121 atcgtattag ctggatatat gcgaatcatt ggaccggtat tgctgaaaaa ttatgataaa
     9181 agaatcatca atatccatcc gtctctttta cctgccttcc ctggtttgca tggcatacgt
     9241 gatgcctttg aagctggagt aaaagaaacg ggtgtcacaa ttcattatat cgaccaaggt
     9301 gtagatactg gaccaatcat ccggcaggaa aaagtgagaa ttgaacagga ggacacgttc
     9361 gattctttag aagaaaaaat ccatagagta gagcatcgaa tctatccaga agtgattagt
     9421 gaaattattg aaaatggagg ctatctgaaa tgacaagagc attgatcagt gtatcagata
     9481 aaactgggat tgtagcattc gcccaaggtt tgattgaaca aggagtagaa atcatttcaa
     9541 caggaggaac aaagcatgcg ttggataaag ctgggatcaa aacgctttcg atcgaagaag
     9601 tgacagggtt tcctgaaatg atggatggcc gtgtcaaaac acttcatcct aaaatccatg
     9661 gtggtctttt aggaagacgc gatctcccta gccatatgga ggcaatggaa acggaaaata
     9721 tccaaccaat cgactttgtt tgtgtcaatt tatatccttt caaagaaacg attatgaaac
     9781 cagatgtcga aatagcagaa gctattgaaa acattgatat cggtggtcct agtatgctcc
     9841 gtagtgcagc aaaaaaccat gaatttgtca cctctgttgt tgatccatct gactatgaag
     9901 aagtactttc agaattacgt cagtatgggc atacttcact ttctttcaga caaaaactag
     9961 cggcaaaagt ctttcgtcat actgcggcat atgatgcact gatcgctcaa tacttaacgg
    10021 attgggtgga agaaaaagaa ccggaaaaat tgacacttac gtatgaacga aaacaaacgc
    10081 ttcgttacgg tgaaaatagc catcaagccg ctgcctttta tcagtccgct cttctggaaa
    10141 aatattcgat cgcagcagca aaacaactcc atgggaaaga actttcttat aataatatcc
    10201 gagatgcaga tgctgcgcta cggatcatga aggaatatga cgagccaacg gtagtcgcct
    10261 tgaaacacat gaatccttgt ggtattggga cagcatctac gattttagaa gcgtgggaag
    10321 cagcgtatga agcagatcct gtttcaatct ttggtgggat catcgttttg aatcgagaag
    10381 tagatctgcc aacagcagaa cagatgcata agctgtttct agaaatcatc attgcccctt
    10441 cttatacaca agaagctttg gaattactgc aaaaaaagaa aaacatccga ttactgacag
    10501 tagactttgc gcaggaagaa aatcaaaatc gagaagaaac ggtctcagtc ttaggtggac
    10561 ttcttgtaca agatcaagat ctggcaatag aaaatgaaga agagtggaaa gtcgtgacaa
    10621 aacgtaagcc gactccagca gaagcaaaag cattagcgtt tgcttggaga gctgtcaaac
    10681 atgtgaaatc taatgcgatc gtactggcaa acgaacgtca gacagtagga atcggagccg
    10741 ggcagatgaa tcgagtcggt tcagtaaaaa ttgcgattga acaagcgcaa gagaaaatgg
    10801 aaggtgcagt tttagctagc gatgcttatt ttccaatgga tgattcggtt gaatatgcag
    10861 ccaaacatgg tataaaagca atcattcagc caggaggaag catcaaagac caagattcta
    10921 ttgatatggc gaataaatac gatctagcaa tggtctttac tgatgttcgg cattttagac
    10981 attagagagg agtcggataa atgaacattt tggtgatcgg tgcaggtggt cgtgaacatg
    11041 caataagtaa aaaactttta gccagtccga aggtggatac ggtgtattgt gcgccaggaa
    11101 atccggggat gacaaaagat ggtatcaatt gtgtcgctat cagtgagcga gatcattcag
    11161 cgttaatcca ttttgttaag aaagaggcta ttgattggag tatcgttggt cctgaagttc
    11221 ctttgttaaa tgggatcgtc gatgatttta tggcggaagg attgaaaatc ttcggtccga
    11281 caaaagcagc agctctgatc gaaggttcga aaagttttgc taaacaatta atggatactt
    11341 atcaaatccc gacagctaaa tccagaagtt ttgaatgtta cgaaaaagca gcagcgtata
    11401 tcaaagaaaa tggtgcgcca atcgtagtga aagcggacgg cttagcagct gggaaaggtg
    11461 tagtcgtggc taccacagtg aaggaagcgc tggatgcggc agaacaaatg ttgaaccagc
    11521 atcgatttgg ggatagtagc aaaaaaatcg tcgtagaaga gtttttggca ggagaagaat
    11581 tctccctttt ggcgtttgtc cgtaatacca aggtgtatcc gatggtcatt tcgcaagatc
    11641 ataagcgggc atttgataac gataaagggc caaatacagg cggaatgggt gcttattcac
    11701 cagtaccgca aatttcaaaa aagatgattg aaaaagctgt ggaagaagtt ttaaaaccag
    11761 cggctttggg aatgaaagaa ctaggacgct ctttcacggg tgttttatat gcgggtttga
    11821 ttgctacgaa ggaaggacca aaagtgatcg aattcaatgc gcgtttcggt gatcctgaaa
    11881 cgcaagtcgt tctttctcgg ttgaagagtg actttgcgca agtaatcgat gatttattag
    11941 agaatcgtac agtagagtta aagtggcagc aagaaggata caatgtaggc gttgtggttg
    12001 cagctgccgg ttaccccgaa gaatatgaga caggaatggt tctaccagat tttctggaag
    12061 aagagctttc tgtttactat gctggagttt cctcaaataa cggcaaacta gttggagcag
    12121 gaggacgtct ttatttagta gaagcatacg gtgagacgat caaagaagca cagaaaaaaa
    12181 tttacaccgc gttaatggat aaaacgacag atggaacatt ttatcgtaca gatattggag
    12241 aaaaagctgc caatagcgaa ctgatcgtga agtaaatcag ctgcttccat ttataaaaaa
    12301 tacaacaaac ataatcggaa gcgccgattt tagtgaagcc ttttcataaa gagtgtggta
    12361 cacttttgtt gtgtatataa ataaggagga gttcagatga aaaaaattca attaggtact
    12421 tctgatcaga aagcagctgc agtgatctta ggctgtatgc gtatcaacaa tgcggaagat
    12481 ccagtaaaag tgattgagac agcttatgaa aacggcattg atttttttga ccatgcagat
    12541 atttatggaa acggtgaatg tgaagaaatc tttgcagatg ctctagcaaa aacatcaatc
    12601 aaacgagaag atctttttat tcaaacaaaa tgtggaatcg ttccaggtgt gatgtttgat
    12661 ttttctaaaa aacatatcat tgaatctgta gaaaaaagtt tgacccgtct gaaaatggat
    12721 tatgtggatg ctttacttct tcatcgtcca gatacattgg tagaaccaga ggaagtagct
    12781 gaagcgtttg atgaattgga acggtcagga aaagttcgtt attttggcgt cagcaatcaa
    12841 aaaccaatgc aaatcgaatt gctgaaaaaa tatgttcgtc aaccattagt agctaatcag
    12901 ctgcaatttg gcttgaaaca tactggaatg atcgatcaag ggattcatgt caatatgaca
    12961 gatgaagcaa gtatcgatca tgacggtagt gtacttgatt attcccgatt gcatgatatg
    13021 acgatccaag catggtcacc ataccagtat ggattttttg aaggtgtatt tatcgggaac
    13081 gaaaaattcc ctgaattaaa ccaaacatta gaagaactag cagctaaata tcaaacgaca
    13141 ccaactggct tggcttctgc ttggatttta cgccatccag ccaatatgca agtgatcgcc
    13201 ggtacgatga acgtagggcg aatcaaggaa atcgctcaag cttcagaaat taatatgtct
    13261 cgggaagatt ggtacgaatt gtaccgtgct gctggaaaca ttttacctta aagattgtca
    13321 taagcaggtc aaaagggcta taaattaaaa agacagaata acttgaaagc tgttttcagc
    13381 ttcgagatat tctgtctttt tttattgcaa atggaaagag tatcattgta cggatttcgt
    13441 ttattagtta tttttgtttt taaggaaaag ctgtgtccga tattcttttg gagaaatccc
    13501 ttgttttttc ttaaataaat aactgaaata gttagggtca ttataaccaa tttcaaaagc
    13561 aatatctgaa atacgatagt ctgattgctg caataatttt tttgctaatt cgattctttg
    13621 ttctgtcaga taatcgatga atgtttggtt aaacgattga gaaaaaatcg tactgaaatg
    13681 agagggactt aatgcaactt gttgagcgac ccaagttaag gacatatctg gatcggtaaa
    13741 attttctttg ataaaatcca atgcatggtt caaaattggc tgatatttgt tccttgaagg
    13801 ctgtgagggc agagcagtta agaaagaaat gaacatataa actgtcgaac ggtaaagcga
    13861 gtcgtcggcg ctgattgcta agcgttgact ggtggatgtg caatgaacca tttcttgcgg
    13921 gattttcttt tctttcaggc agacatgctg gagtagctta gtcaagacaa ataaacgata
    13981 taatcgtgtt cgagttggtg tgccttgtgg tttcgtcaag tgttcgatgt acttagatac
    14041 ttcgtttttc gataatgaag acaaatcaag tgagaggtca aagtaaaaga cagaatcaac
    14101 tggaagtttc gcttcttgaa tcaaatcctc atagctgata atttgttctt taagggaact
    14161 ggcggtttca agtatcatct gcgtgatctc aaaagatgca ggtaattcgc tgattctttc
    14221 tgcgacagga ccaatggcga cagaaaaatt atttttttgt cccttttcta attcatggac
    14281 caacgtttgg gctacctgat aactggtctc gatcacattt tcttcaagag aatcaactat
    14341 taaaaatttg atgaatcgag aggaaacagc agagaagatc acttgttgat tctccctaaa
    14401 taaaaacttc aaataatcac tgagatgagc gtaatcttca aagtctgtac tgacttggtt
    14461 tgctgctaat aatacggtca tttttttccc tgctaaagag cgattgaaag attcagcaag
    14521 tttcaatgtt tcactgatcg taagtgagcc tttgaacaaa ccgttcaaga aatgattttt
    14581 tttcaattcg aaaacaagat cagaagaagt gtgcgtttct ttttgctgca aatctaaagc
    14641 agttactgct ttttctattg cttgtttcaa ttcgctactt ttaatcggtt taagcagata
    14701 atcatctgcc tgtacctgga ttgctccttt catataatcg aaatcgtcga atccactaat
    14761 aaatatgaga cgagtccaag gaagtagttt ccttgcttct tttgaaaaag tcaacccgtc
    14821 catgaaaggc atttggatat cagtcagaat gatgtcgggc tgtaagtcga taattgaaga
    14881 cagtgccatc tcaccatctc cagcttcgcc gacataagtg attggataat aagaaccaag
    14941 gacagttatt tgttcccgca agttattgcg aatgaggtat tcgtcttcga tgataaaaac
    15001 tttatacatg tttgtttcct ccaattgttt tgggagatag agggatcatc agtgtgacag
    15061 ttgttccttc tcggtatttg ctgtcaatcg tgataggttt ttgatcattt ccgtaataaa
    15121 gaaggagacg cttgtttacg ttgtaaagtc cgtagccgat actgaaatct gaatcgatcc
    15181 ctttggctaa ctctttacgt atttctgcca gtttttctgc agggatgcca atcccgttgt
    15241 ctataacttt gaattggatc atctcatcaa ttttgcatgt ggtaatacga ataaatcctt
    15301 tgtgacggac gtacttcgtt ccatggtata cagcattttc aatcaatggc tgcaagatca
    15361 tttttaaaat agaaacagga aaaagtgttt ctggtatatc gatcttatag tcaagcatat
    15421 ggccgtatct tatttttaag atgactagat aatcattgat atggctaatt tctttttcaa
    15481 gaggaatcca gtcttgtcct ttgcttaaag acgtacgaaa aaagccggaa agtgcatggg
    15541 tcatagtcag agcttcttca aagcagtctt gttcgatcaa agaaataatg gcatccaaac
    15601 tgttataaat aaaatgggga gtaatctgag cttgcaatac acgaacttca ctttgtgcta
    15661 aatggtattg tttcaaagcg ttctcccgta aagactggtt tagatcttcc gccattttat
    15721 tcatgctagc ggtaagtgtt tgtaattcca gtgtttccgg cagggttaaa cgaaagccaa
    15781 ggttccctga agaaagttgt tctgacatct cgatcagctg attgatcggt tcttggacct
    15841 ttctctctaa aaagcgacga ttctttttcg catagaaaaa aatggtacta agaactccaa
    15901 gttccactat tgttaataga atgagcgagc ggacaagtgt cgtatttttt ttgcttgcta
    15961 agtcgatctc cacacgaaca aaatcttgta agatatctga cagcagttga gtgacagaat
    16021 ctacctgatt gatgatctct tcattggtat ccataaaatc ttttgccaag atattattga
    16081 tcagctcgtt ttgataatct tccagtgtat cgatcgtacg tagcgataca tctaacgtac
    16141 taatttcttt aagtgtattt gtattatttt tgatctctcg aatatcattc ctaagttctt
    16201 ctaggatatt ttcctcattg aactcatcta cggttacttg gccagaaatc aagtcccaca
    16261 tactttccaa aactttttga tcaaccttca aagcaattgc atctgctttt tggatatttg
    16321 cgatcgtctg ttgataaatc aaaagctgtt gcgaatataa cagtatgctc agtagcaaag
    16381 ggataacagc aaatagaagc atggctttgt ttgcttgggc aaaaatctgt tttatgctta
    16441 aattttttgt catgatactt ctccagtcaa taatttctgg ttgggatgtt agctatattc
    16501 tgctgggaaa agacagtctc ggaaataaaa atatctttag gtaatgtttt tcctgtttga
    16561 atatttccaa aataacgagt aacggtattg gctacatacc agccgacatc aggtttacac
    16621 tcgactacac aattgacgat tcctgagcgt agcttctcga tcatatcttc ttgtccatca
    16681 atagttacaa tgatcaaatc ttctccaggt ttgattgatg tttcttttat cgcagctaaa
    16741 gcaccgtgag tcatttcatc actatggctg tataatacat cgatttcgga taaatcattt
    16801 gtttctaaat atcgtttcat ctggtctttt cctttcatcc gaataaaatc accgacaatt
    16861 cggtgaacaa tagtcatcgg aggtttatgg tcgatggttc catctttttt tagggttggt
    16921 ttacgattga ttgattccaa aaatccttcg gagcgtagct tggtcggtgt ggagttctct
    16981 gaaccagcaa gttctaggat tctgatttcc tgtttctttg aatttgtgaa gtgattgctg
    17041 acaaataaac ccgcccgatt tccttctgct ttgaaactgg ggccaatgtg ggtgacgaaa
    17101 agctcgggat cattcgtttg aatatgtctg tcgacaagta tgacagggat ccctgctcgc
    17161 tttgcttctt ttaatacaga gtcccacccg gattcttgca aaggagtaaa aatgatcata
    17221 tccactttat aggcaatgaa cgtccgtata tcttggattt gccgttcctg attcatgtag
    17281 ccatttcggt agagaacttg gtaatcttct ttttcaagcg cagtgataat tgattcagta
    17341 tgctttttcc gccagctact ctccgttcct gattgcgaaa aaccaacgac tagtgggtca
    17401 ggtgcttcct ttgtattagg tgtacacccc atcatttggt aagcgattac aacaagggct
    17461 aaaaaaaata aaacacgttt cactttaaat cactccatct ggatttgtta gtactatctt
    17521 atcagataaa agggaaaatc aaagtcaaaa ggaatcaatc gaaaaaaatc caagaaactt
    17581 aaaaaaatcc aagacaaaaa aagtacacct aaaaaattta aagtaaatcc cataaaaaaa
    17641 tcaatgttaa cgctttcttt attagctata ctcggtgtat caattgaaat gggggataaa
    17701 aaatgaaaaa caaatttaga aagattgttg ggactatcgc tattttgacc gcaagcattc
    17761 tattaagtgc gtgtggcaat tcaggatctg cagatgatag tacggggtat gtaggaatcg
    17821 ctatgccgac caaatcagca gaaagatgga tcgctgatgg gaacaacatg gtctcggaat
    17881 tagaaaaact gggttataaa acagatcttc aatatggaga agataaagta gaaaatcaag
    17941 tagcacaaat agaaaatatg attacaaaag gggtagatac acttgtgatt gcttctatcg
    18001 atggttcggc tttaactgat gtgttagcta aagcaaaaga agcagatatc aaggtgatcg
    18061 cctatgatcg tttactgatg aattcagaaa atgtcgatta ttacgcgact tttgataatt
    18121 ttggagtagg tgtatcgcaa gcagcttata ttgaagagca cttaggattg aaagaaggaa
    18181 aaggaccatt cacgattgaa ttatttggtg gctcgcctga cgacaacaac gcactaatca
    18241 attacaacgg agtgatgtct gttttacaac catatatgga caacggtcag ttggtcgtgc
    18301 catctggaca gacgagtttc agtcaaatcg ctacgcttcg ctgggatggt tctacggcgc
    18361 aagcaaggat ggataatctg ttaagcgcaa attatactga ccaaacattg gatgctgttt
    18421 tgtcgccata tgatccaata agtttaggaa tcatttcgtc attaaaaggc gtggggtatg
    18481 ggagtgaaag taaaccttta cctgtgatta ctggacaaga tgcgacagtt gctggcgtta
    18541 aatcgatcat tgccggggaa caaacacaaa cgatctttaa agacacacgt atcttagcaa
    18601 aaaatacgat tgaaatgatc aaagcaatca gtgatggaga agaggttccg gtcaatgata
    18661 ctgaaactta tgataatggg gtgaaaactg tccctacgta tttagcaaat acagtatctg
    18721 tcgataaaga taattatcaa gcagagctga tcgacaccga ttattataaa gaatccgatc
    18781 tgaaaaatta agaggaacca aacaaggaac taaagagatc taagtcagag agaggataag
    18841 aattatggcg gattgtattt taaaaatggc agatatcacc aaaaaatttt ctggaatccg
    18901 agcgttagat tgcgtgaatc tgatgattga acgtggggaa atccatgcat tgtgcggaga
    18961 aaacggagcg ggaaaatcaa cattgatgaa tgtcttgagc ggcttgtatc catatggctc
    19021 ctatgacggg gaaatctttt atgatggaaa gctgtgtcaa tttaaagatt tgaaagatag
    19081 tgaagaaaaa ggcatcgtca tcattcatca agaattagca ctaagtcctt atctttcaat
    19141 aaaagagaat atttttctcg gaaatgaaca agcagaaaga ggcgtgattg attgggaact
    19201 gacagaaaag aaaacatatg aactgctgaa taaagtcggt ttgtctatcg acccgaatct
    19261 tctagtatcc cagattggcg tagggcaaca gcaactagtt gaaatagcta aagcattatc
    19321 aaaatccgtc cgtctgttga ttttggatga gcctaccgct gcattgaatg aagaagaaag
    19381 cgcgaatttg ctgagattga tccgagagtt gaaagagcaa ggtgtaacct ccatcattat
    19441 ttcacataag ctgaatgaaa tcgtggatat cgcagatcga atcaccattt tacgggatgg
    19501 acagacgatt gagacactag aaaatgaaat gatcagcgaa gagcatatta ttcgcggaat
    19561 ggtgggcaga gatttagttg atcgttatcc tgaaagaaaa ccggatattg gagaagttta
    19621 ttttaaagta gaagactgga cggtctatca ccctcttgat cacgagcgaa tcataaacga
    19681 ccacatcaac ttgcatttga aaaaaggaga gattgtagga atagctggac tgatgggagc
    19741 gggcagaact gaattcgcta tgagtctatt tggacattct tatggaagca agatatccgg
    19801 acgtatatta aaggaagggg cagagatatc agttaagacc gttccttcag ccattgcaaa
    19861 cggactcgct tatgtctcgg aggaccgaaa atctttagga ctgaatctgc tgatggatat
    19921 tcgggaaaac gtgacgctga gcagtttgga taaaataagt caaaaaggag tattagataa
    19981 agaaaaagaa gtaactgagg cagaaaattt ccggaaaaag atgcgtataa aaacaaactc
    20041 catccatcaa attgtcagta gtcttagtgg tgggaatcaa caaaaagtcg ttttatccaa
    20101 gtggctgatg acacaacccg atattctttt tttggatgag ccaacccgtg ggatagatgt
    20161 tggggcaaaa tatgagatct acacgattat cgaagaaatg gcagcaaatg gcaaagctgt
    20221 ctgtcttatc tcttctgaac ttcctgaaat attagggatg tgtgatcgaa tctatacgat
    20281 gaacgaagga cgtattacag gagaagtctc gcgagaagat gcagatcagg aagtcttgat
    20341 gaaattaatg acgaaagaag aggtgggtta agttggaaca ggcaaaaaat cttacggata
    20401 aaaaagagaa aaaactaaat ttgaaaagta gagcagctga tctttttggt cgctacagca
    20461 tggtgatcat tttagcggga ctacttattg cgtttcaatt gatgacggat ggtattttct
    20521 ggcgtccatt gaatataact aaccttgttc tgcaaaatag ccatatatta gtactagctg
    20581 cagggatgtt attagttgtg ctcttaggtc atgtggattt gtcggtaggg tcagtgatgg
    20641 cgtttgttgg tgcaattgcg ggcatgatga tggtcaatcg ccaaatgaac ccgtggatga
    20701 cagttatcgc atgtcttgca gtaggcgggc tgatcggcgc atggcaaggt ttttgggtag
    20761 cttatgcagg gataccggcg tttatcgtga ctttagcagg attgctgatg tttcgtggac
    20821 tgacacaagt tgttctagga ggccaatctc tagcaccttt tccagctgtt ttccaaaaaa
    20881 tgtctacggg ttatttacca gacttaagcg aaggaagcag ccttcacttc ttgacattga
    20941 ttttaggaat gatcatcacg atcgtgttag ttgttggtca atggcgaaca agaaatcgtc
    21001 ggaaaaatca tttatttgaa gtggaatctc caacagcttt cttggtaaag gcggtattta
    21061 cagcagtcgt tgtactagga ataacttata tctttgcctc ttatcaaggg tatccgatta
    21121 ttttgatcat tcttggtttg atcgttgcag catatgggtt tttgacaaat aaaacagtag
    21181 caggtcgaca gatttatgcg acgggcggaa atcgaaaagc ggctgaacta tcagggatca
    21241 aaacaaagcg aatcacgttc tgggtattcg tcaacatggg agtcatggca gctttagctg
    21301 gattagtttt agctgttcga ttgaatgcgg caacacctca agcaggtaca tcgatggaac
    21361 tggatgcaat ggcggcagta tattttggtg gtgcttcgac ttccggcggc attggaacaa
    21421 tcatgggtgc gattgtcggt ggcttggtca tgggtgtatt gaacaatggc atgtcgattt
    21481 taggcgtcgg ggtagactgg cagcaggcaa tcaaaggatt gatcttgctt ttagccgttg
    21541 tgctagactt gtataataag aaaaaacgaa caacgtagga gaacgccggt tattcagaaa
    21601 aaagactgtg acaagacatt tgtcacagtc tttttttcgc taaaagcgaa cattcattgt
    21661 ttcgtttgta tagactaaaa ataatatatg tttttttacc attcgtataa attctctttt
    21721 tgctcttgat ttttttgctt aattgagtta aattacatag ggcgctaaaa aaagccttga
    21781 ttaaattaat aatgttcgtg ttttgctgga tgtgaacatc aggaggataa tgtaatgaaa
    21841 gtatttgatt acgaagatat tcaattaatt cctaataaat gtatcgtaaa tagccgttct
    21901 gaatgtgata caacggtcac attaggtaaa catacattca aaatgccagt agtgcctgcc
    21961 aacatgcaga cgattattga tgaaacaatc gcagaattct tagcggaaaa tggttatttc
    22021 tatatcatgc atcggtttga tgaagcagct cgaattccgt ttatcaaaaa aatgaaaaag
    22081 cgtggattga ttacctcgat cagtgttggc gtcaagaaag aggaatattc ctttatagag
    22141 aagttggcag aagaatcgct aaatcctgat tatatcacta tcgacatcgc gcatggacat
    22201 gctaactcag ttattgatat gattcagcat attaaaaaat atttgcctga aacttttgtg
    22261 attgccggta atgttggtac accggaagcc gtccgtgaat tggaaaatgc gggcgcagat
    22321 gcaacaaaag tcgggattgg tccaggaaaa gtatgtatta caaaaatcaa gacaggcttt
    22381 gggactggag gctggcaact ggcagctctt agatggtgtg cgaaagcagc tcgcaagccg
    22441 atcatcgctg acggtgggat cagaacacat ggcgatattg caaaatctgt ccgttttggt
    22501 gcgacaatgg tcatgattgg ttcattgttt gccggtcacg aggaatctcc tggcgaaaca
    22561 aaagtcgaaa atggaatcgt gtataaagaa tactttggtt ctgcttcaga attccaaaaa
    22621 ggcgaaaaac gaaatgttga aggaaagaaa atctggatcc aacacaaagg atctttgaaa
    22681 gatacactag tggaaatgca acaagacctt caatcatcta tttcttatgc tggaggaaga
    22741 gatctggaag ccattcgaaa agttgactat gtcatcgtga aaaattcgat ttttaatgga
    22801 gacacaatct aaaaaataaa taaaatatta ggatgttgtg aaaaagttgt cctgcattaa
    22861 gtaataagaa cagccaaaca aaaatagttc tttatatgtt ttgtttggct ggaattatta
    22921 tcgcagatac aagcttttga acatcattat tcagaataag ggactgtgat aagatttttg
    22981 tcacagtccc ttatgttttg caaaaaaagt ttttcttttt tctcgtaaat tcggtaaaat
    23041 caaaagaaaa aatagacaat agaaaagaga agcttatgtg ggaaaaagta gagaaaaaac
    23101 aaggaccgat ttatcgtcaa attatggaac agattatgag agaaatcgaa aacggaagga
    23161 tcaatcctgg aagtcaactt gaatcagaac gaaaactagg gcaacttttt ggtgtgaatc
    23221 gttccaccgt tgtccatgca ttggaagagc ttagggagct tggggtacta acgagcaaac
    23281 gtggcagcgg atggtttgtt aatcagactg aatgggggaa attcgctgtt ccaagaatcg
    23341 attggcggca aatgatctct cctcgttatg agcagacgga ctggtatgag caaaaaataa
    23401 gagaagcaaa gcagatccaa aaaacatcgt ttcttgattt atacgctagc gagatgccag
    23461 atgagctttt gccgaatttt gagtttcctt cttattctct tgaagaaatc ttagcggaag
    23521 aaaaagaaat ggatctttta ggctatactc cgttacgtaa caaaatcaaa cggtatttgg
    23581 aaagtaaatt tgaattggca ttttccccca accagcttct tgtgactggt ggcggtcagc
    23641 aagcaatctt tttgatttta caaacgattc tttcagctgg agaggggata gctgtagagt
    23701 caccttcttt tttttaccga ttacctcttt tcaaagcttc aggaacgcgt ttatttggga
    23761 taccgatgga tgaagaaggg atcgacttag aaaaactgaa agaatcgatc cataaaaata
    23821 aattgaaagc agtgcttatc aacccgaatt ttcaaaaccc tacaggaaaa gtgatgagtc
    23881 aaaaacgacg agaaaagcta gtagaattgt gtcgacagta tcggcttcca attattgaag
    23941 acgatgtatt tacggatctt tcatttctaa accaagaaga agtccttcct atacgaacga
    24001 ttgatccgga taatgtgtta tacgtcggtt cgttatctag ggttttagga aaaacaacga
    24061 aaatcggttg ggtgatagga cctgagactt ttatctcgca actagctcaa gcgcaagaaa
    24121 tgatggagtt ttcgatgaat atccttacac aaatcacagt ggctaatgta tttaatgaag
    24181 caattgattt gaaaatgcaa gatgtaagaa aaaagttgaa agaaaaaagc cgactgataa
    24241 gcgcttgggg agattcacaa tcgtttttca agcttcattc aattttaggt ggttactatg
    24301 cctggattac ttgggatggg aaaaaactag accccgaact agcagaaaaa gtagtagata
    24361 tgggtcttgg aattgcacca agtctacttt ttgggcaaga tataaatgga atcagaatca
    24421 attacagtag gctggatgaa aaccaaatac cgttgtttta ttcaaagatg aaaattctag
    24481 aaaagtggtt aacagaataa tttcacaaag taaatagatt aaagaatata acagcttgga
    24541 tttaaatttc aagctgtttc ttttttttcg gaaaaagaat acgatataaa gaaactaaga
    24601 aaaaaccatt atttgtacgt ccgttcacga tgtgtaaata aaataaatgt ttgcaacaaa
    24661 actgtataac accaacgttt ttaaatagaa acagatggtt gtaagtttga ttagaaaata
    24721 gaaaaaatgc agatgaaata taaaacgctt tccttattgt gattttattc actaatgctg
    24781 ttaatatgcg gattggcata atttcctttc atcgtgaaaa tttgtgcttt tagcttgtat
    24841 ttctcataaa tccatgataa tttattaatg aagacggtta aatagtgata cagttttatt
    24901 cactgtttca atcgcagaat gaagagattt tcttatagat gagagaggaa tgtgtgacaa
    24961 atggcaaaca aaaaagcacc gatcgacttc caagcactgc tagatgaagt caatgcagat
    25021 ttcccagtat atcaagcact tgatcaagat ggaaacgtag ttaacaaaga tttagtacct
    25081 gatttatccg atgatgaact cgttgaatta atgactcata tggtatggtc tcgtgtatta
    25141 gaccaacgtt caacagcgct gaaccgccaa ggacgcctag gcttttttgc accaactgct
    25201 ggacaagagg caagtcagct ggctagtgct tacgcatttg ataaagaaga tgttcttctt
    25261 ccaggttatc gggatgtacc tcaactagta aaacatggtt tacctttatc tcaagctttt
    25321 ttatggtctc gaggacatgc tgcaggtaat tcttatccgg aagaattgaa agctctacca
    25381 ccacaaatta tcatcggtgc acaatatgta caagcagccg gtgttgcatt aggtttgaag
    25441 aaacgaaaca agaaaaatgt cgtcttcact tatactggtg atggcggttc atcacaaggt
    25501 gatttttacg aagcaatcaa tttcgctggc gcttaccatg caaatgccgt attttatatc
    25561 caaaacaacg ggtatgcgat ctctacacct cgtgaaaaac aaactgcagc aaaaacactt
    25621 gcacaaaaag ctgtcgcagc tgggattcct ggtattcaag tcgatggaat ggatccgctt
    25681 gcagtatata ctgtatctaa aatggcacgg gaatgggcga tcagtggaaa tggaccagtc
    25741 ctaatcgaaa cactgactta tcgttatgga ccacatacac tatctggtga cgatccaacc
    25801 cgctaccgta caaaagacac agatgacgat tggcagaaaa aagatccatt gatccgcttc
    25861 cgtaaatatt tgacggaaaa aggactttgg tctgaagaaa aagaagaaca agtcatcgaa
    25921 gcaactaaag aagaaatcaa agcagctatt gctgaagccg acaaagtacc aaaacaaaag
    25981 gtttcagatt tcttgaaaaa catgttcgaa gtatctccac aatcaatcaa agaacaaatt
    26041 gcaatttatg aagcgaagga gtcgaaataa tcatggcaca aaaaacaatg atccaagcaa
    26101 tcacagatgc cttagcactt gaactagaaa atgacgagaa tgtcgttgtt tttggtgaag
    26161 atgttgggaa aaacggagga gttttccggg caactgaagg attgcaggaa aagttcggag
    26221 aagaccgagt attcgatact cctttagctg aatctggaat cgcaggattg agtttcggtc
    26281 ttgcgttaga aggatttcgt ccagttcctg aaatccaatt ctttggtttt attttcgaag
    26341 caatggacga agtagtagct caaatggctc gtacaagata tcgaatgagc ggcactagaa
    26401 atctacccat cacaattcgt tcgccatttg gtggcggtgt tcatacacca gaattgcatt
    26461 cggataattt agaaggtttg atcgctcagt ctcctggtat tcgagtagtg attccttcca
    26521 acccttatga tgcaaaagga ctattgattt cttctattag aagtaacgat ccggttgttt
    26581 tcctagaaca catgaaacta tatcgctcat tccgagaaga agtgccggat gaagcttacg
    26641 aagttccttt ggataaagca gcagttactc gtgagggaac agatgtatct attatcacat
    26701 acggagcaat ggtgcgtgaa gcaatcaaag cagcagacaa tcttgcaaaa gaaaatatat
    26761 ctgttgaaat cattgatctt cgtacagttg cgccattaga tgtagaaacg atcattcaat
    26821 ccgtggaaaa aactggtcgc gtagttgttg tccaagaagc acaacgacaa gcgggcgttg
    26881 ccgcgcaagt agtttctgag atctcagaac gcgctatcct ttcattagaa gccccaatcg
    26941 gacgagtttc tgcacctgat acagtcttcc catttggaca agcagaaaat gtctggttgc
    27001 caaatgcaaa agatatcgaa gacaaagtaa aagaaatcgc ggaattttaa gaaaagaagg
    27061 gacgaaacaa agtcatggct tatcaattta aattgccgga tatcggtgaa ggaatcgcag
    27121 aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa gatgatacat
    27181 tactagaaat tcaaaatgac aaatcagtag aagaaatccc gtcacctgtt acaggaacgg
    27241 ttaaaaatgt gattgttcca gaaggtaccg tagcaaatgt tggggatgta ttagtagaaa
    27301 tcgatgcacc aggacacgaa gacaatgaag gggacagtgg tgtagccgca gaatcccaaa
    27361 ctccagctaa accggctgct gaacccactg ttgatacaga atcagctgga tcgtcaagcg
    27421 aaggagtctt ccaatttaag ttaccggata tcggtgaagg aatcgcagaa ggtgaaatcg
    27481 tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta ctagaagttc
    27541 aaaatgacaa atcagtagaa gaaattccgt cacctgtcac agggacagtt aaaaacgtga
    27601 ttgttccaga aggcactgta gcaaatgttg gggatgtatt agtagaaatc gatgctcctg
    27661 gacacaatag tgcaccgtca actagtgcgc catcagctga agcaccaaaa gaaaaagtgg
    27721 aaacatcagg ttcagcaagt gtcgttgaag ctgccgatcc aaataaacgt gtattggcta
    27781 tgccatctgt tcgtcaattt gcccgtgaaa aagatgtaga tatctcacaa gtaactgcaa
    27841 ctggaaaagg tggcagagta acaaaagagg acatcgaaaa cttcttggca ggtggtccaa
    27901 gcagtgcacc agcgaaatca gaagcgccag aagcggcagc accgaaagaa gcagctcctg
    27961 cagcagaaag caaaccagct gcaccagcaa aaccattcaa gtccaactta ggagacttgg
    28021 aagaacgtgt agcaatgacg ccaacacgta aagctatcgc taaagcaatg gtcaacagta
    28081 agcatacagc tccacacgtg acattgcatg atgaagtaga agtgtctaaa ctatgggata
    28141 atcggaaacg atttaaagaa gttgctgctg caaatggtac gaaacttact ttcttgccat
    28201 atgtcgtgaa agctttaaca gcgactgtta agaaataccc agtattgaat gcatctatcg
    28261 acgatgcaaa ccaagaaatc gtttataaac attactacaa tatcggtatt gcaactgata
    28321 cggatcacgg actatatgta ccaaatgtga aagatgcaga ccgcaaagga atgtttgcta
    28381 ttgcagacga aatcaatgag aaagcaaaat tagctcatga cggcaaacta tctgcagaag
    28441 atatgcgcaa tggaacaatt acgatcagca atatcggatc tgtaggtggc ggttggttta
    28501 caccagtcat caactatcca gaagtagcta ttttaggtgt aggaacgatt gcacaacaac
    28561 caatcgtaaa tgctgaaggc gaaatcgttg tcggtcgtgt gatgaaactg tcattaagtt
    28621 tcgatcaccg tatcgtagat ggagcaactg cgcaacaagc aatgaataat atcaaacgtc
    28681 ttttagctga tccagagcta ttaatgatgg aaggatgata aacaaatggt agttggagat
    28741 tttgctgtag aattagatac agttgtaatt ggtgcgggac ctggtggcta tgtagctgcc
    28801 atccgcgctg ctgaaatggg acaaaaagta gcaatcattg aaagagagta tattggcggt
    28861 gtttgtttga atgtcggctg tattccttca aaagcactga tagctgcagg acaccattat
    28921 caagagtcac tagattctga aatgtttgga gtaactagtg aaaacgtcaa actagatttt
    28981 gcaaaaacac aagaatggaa agaaaacaaa gttgtccata cgttgacttc tggtgttggt
    29041 ttcctattaa aaaaacataa ggttgaaaca atcgaaggag aagcgttctt cgtagatgat
    29101 cacacactac gcgtcattca tccagattca gctcaaactt attcgttcaa taacgcaatc
    29161 atcgcaacag gttcccgtcc aatcgagatt ccaggattta aattcggagg acgtgtgtta
    29221 gattctactg gcggattagc attgaaagaa gtacctaaga aatttgtgat catcggtggc
    29281 ggtgtgattg gtgccgaatt gggcggagcg tacgcaaact taggagcaga agtaacgatt
    29341 cttgaaggct caccacaaat tttaccaact tacgaaaaag atctagtaaa attagtagaa
    29401 gatgatttca agaaaaaagg cgtaacagtc gtaacgaatg caatggcgaa agaagcagtc
    29461 gataacggtg acagcgtgac agtgaaatat gcagtggacg ggaaagaaga atccgttact
    29521 gctgattatg tgatggtcac tgtaggtcgt cgtccgaata cagatgatat gggcttagaa
    29581 caagcgggtg tcgaagtagg agaacgagga ttgatcacgg tggataaaca aggacgtacg
    29641 aacgtaccta acatctatgc catcggtgat atcgttccag gggcggcatt agcacacaaa
    29701 gccagttatg aagcaaaaat cgctgctgaa gcaatctctg gcaaaaaagt agctgtagat
    29761 tataaagcaa tgccggcagt agccttcact gatcctgaat tggcttctgt aggtatgacg
    29821 atcaaagaag caaaagatgc tggtatcgaa gcaaaagcat ataaattccc attttcaggg
    29881 aacggtcggg cattatcgct tggtaaaaca gaaggattca tccgtctagt tacgacgatc
    29941 gaagataacg tgcttattgg cgcgcaaatc ggtggagtcg gcgcaagtga tatggtctct
    30001 gaattagctt tagcaatcga atcaggaatg aatgctgaag atatcgcctt gacgattcat
    30061 cctcatccat ctcttggcga aatcgtcatg gatgcttcgg aattagcatt aggtttacca
    30121 atccatattt gatttgaata atacaggcga aacgcaactg aaaaatcagt tgcgttttgt
    30181 tttttagaat tatcaataat attattatgt aaacttaaaa atatgacaac ttcttggttt
    30241 attaccactt cctatgctac actgaagagg aatatgaaat aggagagttg aagatgcaag
    30301 aatatcagta tccacttgat ttagattgga caacagacga aatggtcatt gtaatgaaga
    30361 tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa caatgaagct tttttgaaga
    30421 cttatcagca gtttaaaact gttgtcaaat ccattggtga ggaaaaacga ttgggacggg
    30481 aatttgaaaa agcttccggt tattctttat atcgtacagt gcagcaagca aaaaaaaatc
    30541 caaacaaaac tttgagaatg aacgggtgag ataaatggaa cagatgattg atcatatcaa
    30601 gtcctggctt gaagaagctg gtgagaaaat caaatcttct caacaaacat taacaatctc
    30661 gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat cgaaacatcc agaattttct
    30721 aatcgaaaaa atccattcct ttgatccaga ggcgaaaata ttggcagaag aagaagggtg
    30781 taatcaatta tcggatctcg atggaagagt ctttatcatc gatcctattg atggcacctt
    30841 gaattttgtg attgaagggg aaaatttttg tattatgctc gctgtttatg aatccggcgt
    30901 tggaaagctt ggtttcattt atgacgtgat gagaaatgaa cttttttggg gtggaaaagg
    30961 aataggcgtg tataaaaatg atgagccatt gcctagacca gcaaataaat ccttatcgga
    31021 aggcttactt ggtgtgaata gttatctttt cggtcataat aaatatggaa tccgagaaat
    31081 tgcggaaagc agcatgggga cacgtatgta cggatgcgct ggtcttgaac tagtggcaat
    31141 cttgaaagga aaccacattg cttatatttc aaaccttagc ccttgggatt atgcagcggg
    31201 aaatgtctta ttggaagagt ttggtatggt aacaagcggt ctttttggta ctcctttgac
    31261 atttgaaaaa agggaatact atttagcagg gacttcttgt gcatatgcag aaatcgcaga
    31321 ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat tcacaaaaat gataaaaacg
    31381 agcaaaagat taattttctc gaaaaaatga aaacatttta ctatatttca tgaaaaaagt
    31441 ctgctataat tagcagtaga gtaaaatttg ccctgtcata aagaaccaat aaaaaaggag
    31501 ttagttacat tgaattatag aaacgatatc cgtaacgtcg caattattgc ccacgttgac
    31561 cacggaaaaa caacattagt tgatgaacta ttaaaacaat cagatacatt agacgcacat
    31621 acgcaattgc aagaacgtgc gatggactca aatgcgctag aaaaagaacg tggtatcacg
    31681 attttagcca aaaacacagc tgtagactat aaaggtattc gcgtaaacat catggatact
    31741 ccaggacacg cggacttcgg tggcgaagta gaacggatca tgaaaatggt tgacggcgtt
    31801 gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa cacgttttgt attgaaaaaa
    31861 gcactggaac aacatatcac accaatcgtt gtcgtaaaca agattgacaa accttctgct
    31921 cgtcctgagc atgtcgtaga tgaagttctt gaactattca tcgaattagg agcagatgat
    31981 gatcagttag actttcctgt gatttatgca tctgcactaa atggtacgtc aagcttgtca
    32041 gatgatccag cagatcaaga accaaccatg gcacctattt ttgatacaat tattgaaaaa
    32101 atccctgctc ctgtcgataa cagcgacgaa ccattacaat tccaagtttc attacttgac
    32161 tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat ttagaggaac aatcaaagta
    32221 ggagaccaag tagcattaat taaattagac ggaacagtta agaaattccg tgtaacgaaa
    32281 ttatttggtt tctttggact aaaacgtcta gagattcaag aagcaaaagc tggagacttg
    32341 attgctgtct caggtatgga agatatcttt gtcggtgaaa cagttactcc tgtggatcat
    32401 caagatgcgt tacctatcct tcacattgac gaaccaacat tgcaaatgac tttcttagtt
    32461 aataattcac catttgccgg tcgagaaggt aaattcgtaa ccgctcgtaa gattgaagaa
    32521 cgtttgatgg cagaacttca aacagatgta tccttacgag ttgaaccaac aaactctcca
    32581 gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt ctatcttgat tgaaaacatg
    32641 cgccgtgagg gatacgaatt gcaagtatct cgtccagaag ttattgaaaa agaaatcgac
    32701 ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata caccagaaga atatatgggt
    32761 agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc aagatatggt ccatacagga
    32821 aacggacaaa tccgtttgac attcttaaca cctgcccgcg gtttgattgg ttattcaact
    32881 gaattccttt caatgacacg tggttacgga atcatgaacc atacttttga ccaatatctg
    32941 ccaatgcttc ctggtcaaat tggcggacgt caccaagggg cgcttgtttc gatcgataca
    33001 ggaaaagcaa cgacttattc tatcatgagt atcgaagaac gcggtacagt cttcgtagaa
    33061 ccaggtacag aagtatatga aggaatgatt atcggggaaa acagccgtga taatgatttg
    33121 actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac ggtctgctac taaagaccaa
    33181 acatctgtca tcaaaaaacc aaaacaattg acgttggaag aatctttgga attcttgaat
    33241 gatgatgaat attgcgaagt aacaccagaa agcattcgct tgagaaaaca aattttagag
    33301 aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat ctgtaaaagg atgaagagag
    33361 aaaccagctt gttataaaaa atagctggtt tctttttttg tccaactctt ttctgtggag
    33421 aagtcatctt tgtcaatgga cataaattca gaaaatacgc cgaaagacgt ttaaaagact
    33481 tttaaaaaac ttatttctag agagtttttg tttcataata agataatgtt tcacgaaagg
    33541 agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc aagagaaagg agcgggaaga
    33601 tgaacgactt attaaaacaa tactttggtt atgatgagtt tagacctggt caaaaagaaa
    33661 ttatccagaa agtcattgat caggaaaatg tattaggaat catgcctacc ggaagtggga
    33721 aatcaatctg ttaccagctt ccagctttac tattagatgg gcttactgtg gttgtgtccc
    33781 ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa tcaattaggt atacccgcca
    33841 cttttatcaa tagctcgtta gatggttacg aaacagctag acgcttccaa gaaatcgata
    33901 gacagcagta ccgtttactt tatatcgctc ctgaacgttt tatcatgccg gattttatcc
    33961 aagcaatgaa gcgatggaac gtccgtatga tcgctattga cgaggcacat tgtatctctc
    34021 agtgggggca tgatttccgc ccaagttatt tacagatggc gaaccagcta gatcaactgc
    34081 caaatcgtcc tgtaattgtt gctttgacag ctacagcgac tgtgcaagta gctgctgaca
    34141 taaaaagact tttaaaaatt ccagaaaaca accatattca gactggtttt gaacgagaaa
    34201 atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca atatctaatc gaatatttaa
    34261 agatcaacaa aaatcaatct ggtatcattt atgcagcaac gagaaaagaa gtagaccgtc
    34321 tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag atatcatggt ggtctaaatg
    34381 agaatgagcg aacagaaatg caagaagcct ttttatacga tcgcttgcag ctgattgtag
    34441 cgacaaatgc atttggtatg ggtataaata aaagcaatgt ccgttttgtt atccattatc
    34501 aaattcctgg ctctctagaa gcatactatc aggaagctgg tcgggcggga agagatggtc
    34561 tttccagtga agccatcctg cttttctctc ctcaagatat ccaagtccaa aagtttttta
    34621 tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga atatgaaaaa ttaaaagcga
    34681 tgacagaata cgtgtatatt gagtcctgtc ttcagcaata catcctaaac tattttggcg
    34741 agacaagcag tccgtgcaac cgttgtggta actgtttaga tgacagagaa atagtagaag
    34801 taacgacaga agcacagatg gttctttctt gcttaaaacg tatgggtgag aactatggta
    34861 aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca aaagctgcaa gctttaggtt
    34921 ttggacattt atctacctat ggactgatga aaaaccaatc gcaaaaagaa acgatgcagt
    34981 taattgaata tttgatatcg aatggctatc ttctgaccat caatggtgag tatcctgttt
    35041 tgaaagtaac agaaagaggg atacaagtat tgaaaggaca agaatctgtt tatcgaaagg
    35101 aacccaagaa agtccagcag ctatcggatg aagaaaccga tacactattt gaagtgctgc
    35161 gggaactgcg aacggatcta gcatcagaag caggcgttcc cccgtatgtc gtattttcag
    35221 attccacatt gaaagaaatg agccgaatac gtccatcaag cagattggag atgcttcaga
    35281 taaaaggagt tggccaaagc aagcttgata aatatggcga agccttttta tctcggataa
    35341 aaaatgcgga ctctaacgtt ctggataagt agaaagaacg aatacgtaaa aatgaataaa
    35401 aacaactggt taaatctgtt ttttttgctc cttatgaaaa tcaaatatgt tataattaag
    35461 gaattagaat gggaggtaag cacagatgga ttctttgtcg aaaaaacttg cgatccaagt
    35521 attaaatgat gaagcagaac ggatcaaacg attaatccga aatcaaaaca acagtctctg
    35581 tatctcacaa tgcaaagcct tcgaagaagt ggtagataca cagatgtacg gcttttcccg
    35641 gcaagtcggt tttgctgtcc gtataggagt aatcggaaag aacgagggcc agctgatgtt
    35701 aagtgagcta gaacgagaat tgaatcagtt atatagtgag gtttaccaag aaatttatga
    35761 taaaaaagaa gttggcaagg aggaatagac ttgcctaaga aagtgaaaaa aaggcatcta
    35821 ttagactata gcatcttgat tccctattta attttatgtg tattgggatt gatcatggta
    35881 tatagttcta cgtcgtatct attattagaa aacggacaaa atccgtccgc atcagtcatt
    35941 aaccagagta ttttttgggt gcttagcttg attgctatcg cactacttta taaaatgaaa
    36001 acagatgttt tgaaaaacca gcgtttgatc atggcagcca tcgcagtttt gacgattttg
    36061 ctgctgattg ttgtgttttt tggtaaggag atcaatgggg cgaaaggttg gctgcaaatt
    36121 gcaggattct cgatccaacc tgccgaatac ctaaaaatca tttctatctg gtacctgtct
    36181 ctgactttat ctaaaagaca aaacagtgta caaaaagatt ttcttgggac tgtgaaacgt
    36241 ccacttgcaa tggttatcgg acttactgca ttagtcgcta ttcttcccga ttttggtaat
    36301 gcggcagtta tctttttgat tattttggtt ctgcttttag ccagcggagt caattatgtg
    36361 tatactttga ttgtaggagt aggaggcttt tgtctaagta cttttacgat ctggctgatc
    36421 aatatcacaa acggaaagat cttacccgga cgtttacagt atatctataa tcgatttgct
    36481 atttaccaaa atcctttctc agacgaatta aataaagggc atcaactagt caacggctac
    36541 tatgccatgt tcaacggagg actatttggc aggggattag gtaacagtat ccagaaaaaa
    36601 ggatttttac aagaagcaca aaccgatttt atttatgcaa tcgtagtgga agaactggga
    36661 gtgattatgg ggattttgat attagcgttg ctattcttta tgattgtgcg cattgtcctt
    36721 gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt gcattgggat cggcgcaatg
    36781 tttttgatcc aagtatttgt caatcttgga ggaattactg gcgttattcc tttgacaggg
    36841 atcacgttcc catttttaag tcaaggaggc tccagtttgc tgatgctttc gatttgtgtc
    36901 ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga gcttaggttt ataataaaaa
    36961 aagatgtagg agtggttcga tgaaaaaagt tttagttgct aacaggggtg aaatcgctgt
    37021 acgtgtattt cgtgcttgta cagaattagg aattaaaact gttggaatct atgcagaaga
    37081 agatgaatat tccgtacacc gtttcaaagc ggatgaagca tatttagtag gacaaggaaa
    37141 aaaaccgatt gatgcgtatc tagatattga agggatcatt tctatcgcga aagaatgcgg
    37201 agctgacgct attcatccgg gttacggtct tttgtcggaa aacttgaatt ttgcgcaacg
    37261 gtgcgaagaa gaaggcatca tttttgttgg acccaaactg catcatttag atatttttgg
    37321 agataaaatc aaagcaaaag ctgcagctat cgaagcgggg attgcttcga ttccaggcac
    37381 agatggacca atcgcttcta tagacgatgc cttggagttt gcgaaacaat acggttatcc
    37441 gattatgatc aaggcagctc taggcggcgg cggacgcggc atgcgtgtag ctcacgatga
    37501 aaagagtgca agagagggct acgaacgtgc gaaaagcgaa gcaaaagcag cctttggtag
    37561 tgatgaagtg tatgttgaaa aatacatcgc taatcctaag catatcgaag tacagattct
    37621 cggggatacg catggaaacg tcatccatct ttttgaaaga gattgttctg ttcagcgccg
    37681 tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg aatgaacagc aacgtcaaaa
    37741 aatctgccag gcagctgtcc aattgatgaa atatgtcggg tatgtcaacg caggtacagt
    37801 agaatttctt gttgaaggag atgattttta ctttatcgaa gtcaaccctc gcgtccaagt
    37861 ggaacatacg atcacagaaa tgattacaga tattgatatc gtaacaacgc aactgttgat
    37921 tgctcaaggt ttggatctgc ataaagaaat cggattgcct cagcaagaag gaattaaatt
    37981 aaatggttca gccattcaat gccgaatcac tacagaagac ccgttgaata actttttacc
    38041 agacacaggg aaaatcgata cgtatcgttc acctggtggt tttggcgtac gtctggatgt
    38101 cggaaatgct tacgcgggct atgtagtgac accgtatttt gattcattgc tagtaaaagt
    38161 atgtacgcat ggtgctacct ttgaaacagc cattcagaaa atggaacgtt gcttgaaaga
    38221 atttagaatc cgtggagtga aaacaaacat tccatttatg ctcaatgtca tcacacatcc
    38281 tgaattccaa tcaggaaatg caaaaacaac cttcattgat agtacagcaa cattatttga
    38341 atttcctcga ctacgagacc gcggaaacaa aacgatgaaa tatatcggtg aaattactgt
    38401 gaatggtttt ccaggtatcg aaagtggcga aaaaccattt tatgaagaac cacgtatgcc
    38461 taaagatcta atcacaagaa ctgattacgt aacggctaag aatgtattag atgcgaaggg
    38521 tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat ttgttgttga cggatacgac
    38581 atttagagac gcccatcaaa gtctgctagc aactcgtgtt cggacaaagg atttcaagca
    38641 gattgcacgt ttgactggag aaggtcttcc tgaactcttt tcaagtgaga tgtggggggg
    38701 agcaacattt gatgtggctt accgcttttt gaatgaagat ccttggcaac ggttgagaaa
    38761 aattcgttct ttaatgccta atacgttgct gcaaatgctt ttcagaggtt caaatgccgt
    38821 tgggtattct aattatcctg acaatgtatt ggtggaattt gtcaaagaag cagcagcgca
    38881 aggaatcgat gtatttcgaa tctttgatag tttgaactgg actccacaaa tggaaaaaag
    38941 tatccaagct gtacgggata ctggtaaaat cgcagaggca gctatttgct atactggcga
    39001 tatcaacgat ccaagtcggg caaaatacaa tgttcagtat tataaagaca tggcaaagga
    39061 actagaacaa ctgggtgcgc atatcattgc aatcaaagat atggctgggc tgcttaaacc
    39121 gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg acagatctgc caatccatct
    39181 gcatactcat gataccagcg ggaatggaat catcacttat tctgctgctt caaaagcagg
    39241 agtagacatc gttgacgtgg caatgagtgc aatgagtggg aatactagtc agcctagcat
    39301 gagcagcttg tattatgcac tggtcaatgg tccacggctg ccagaaatca cgattgaaaa
    39361 tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg tattacaaac cgtttgaaaa
    39421 cggcttgaat gctcctgaaa cagaagtata tatgcatgaa atgccaggag gacagtattc
    39481 gaatctacag caacaggcaa aagctgtcgg attaggtcat cgctgggatg aaatcaaaca
    39541 aatgtatcat actgtcaact tgatgtttgg cgatatcgtt aaagtaactc catcatctaa
    39601 agtagttgga gatatggcac tatttatggt ccaaaatgat cttacagaag aagatattta
    39661 tgaaaaaggc gaaacactaa gtttccctga atctgttgtg actttcttcc aaggagaact
    39721 agggcaaccc gttggcggat ttcctaaaaa attgcaaaag attattctta aaggcagacc
    39781 tgcattgaat gaacgtccag gtcttttggc tgaatcagtc gacttcaacg aagtaaaaaa
    39841 agaattggca gaaaaaattg gctatgaacc aaaacaagac gaagtattaa gctatttgat
    39901 gtatccgcaa gtctttttgg actatcaaaa agcatataat caatttggcg atgttacatt
    39961 actggacaca ccaacatttt tccaaggtat acgtttgggt gaaacaatca atgtacaaat
    40021 cgaacgcgga aaaacgctta tcattcgttt agatgaaatc ggagaacccg atatcgaagg
    40081 gaatcgcgta ctgttcttca atttgaatgg acaaagacgt gaaatcacta tcaaagataa
    40141 ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa ccgacaaacc gagaacaaat
    40201 tggagcaacg atgtctggtt ctgttttgga tgtattagtg aaaaaaggcg ataacgttaa
    40261 aaaaggtgat acgttgatga ttactgaagc gatgaaaatg gaaacggcca tcgaagcacg
    40321 ttttgacgga gaagtcgcac atgtatatgt gtcttcagga gacactattt cgtctggtga
    40381 ccttttgatt gaagtgactg agaaataata agcgaaaggt ggggaaacat gaaaagactg
    40441 ggtttgtttt ggctgattct gctattagtt attgtggcat tctacatgga accagtcttg
    40501 tttccgccaa aacttgaagt gaaggaaaac tctgatcaac aaatatcaaa ccaaatgaca
    40561 acgtggaaat accaggagct taaagcaact ggtttttcta cttatatcgg aaaaccgatc
    40621 gatcagctgg aaaaggattt tggccaagcc tatgatcgat taactagcgg atttggtttt
    40681 gaaacaagat attatgaaaa taaagctagt catctcacat ttgaagcgaa catccaagac
    40741 agcagagtca caactgttaa agtactgcaa acagggcctg ctgatatcgc gccgttttat
    40801 ttagggatga cgatgcaaga tcttacgaaa atcactacta tctatactaa tttcactttt
    40861 gagtaccatg atacagaagt tggaatcgaa ctaatggaag aggatatgaa ttatcgtccg
    40921 ctaattgctt ttgataacga cacttttgct attttatttt ttgatcaaac aacaaacggc
    40981 ctattttcag ttgtgtattt agataaagat agtcttttga aactattgcc atatcaagtg
    41041 tttggtgaag gccttccaca ttatcaagca gataaatcgg ctgattggga atcaatcaat
    41101 cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc ttagaagaga agacgaactt
    41161 cccatataca atcaaacact tgcctttcaa aaccacagtc agctgctact acatgatttt
    41221 ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg ctgattggca aaaatcgcta
    41281 ggcagtgctc aaacgacagt gaaattctca ctgacgaatg atgaattgga gaagttaatc
    41341 agtaaacaaa aattagaaaa tactagtggg ctatttattc atccggcgat tgacgctact
    41401 ttttcgtttt tatattggta cagcgatcct tacaaccatg accgtttcat ggacccggga
    41461 acggattctg taggcattgc tttttctaaa gaaaatatgt tagtcttatt gcaagaagaa
    41521 aacggagaaa gtagtgatag cagtgatcgt taacgaagag ctgtttcaac tggaggatca
    41581 atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg atggcttctt atcttgaaca
    41641 taaacagtca atggaagaat ctgcaaaagt ttgccagttg aaagaagcgt tcttgtctaa
    41701 gaaagaagca tttgaaaatg tagcggcata tggtgagtat gcacctgatt atcgtgaaaa
    41761 aaaacgagct gttcgaaaag caaaaagaca gcttgatatg gatgagcatg ttgcagcttt
    41821 ccgatatagt gaaacacagc tgcaaagtct gttggatcaa attgtccaag tacttgccga
    41881 agaagtttct aatgaaatca aagtagatgc aggtagcccg ttttttgaaa cgaaaaaaca
    41941 ctttggatgc ggaggtaatt gtcatgcaat tagacaatag tgaatttgaa ataacaaaac
    42001 gacgcgggct gatcgtatgg gtctacagct taaaacaatt aaagaatctg aaaaagttcg
    42061 gcttgttgca ttatgtctca agaagaatga aatatgtcgt gctttactta aatgaagaga
    42121 cattggaaga aacagaagaa cgaatccgta aattacattt tgttcgaaac gtagaacgtt
    42181 cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat aggaaacaaa acagtattaa
    42241 aagacgaagg tttcgaaatt gaagagctaa atacagaaat ccgattagct gaacatcttt
    42301 aaaaatctct tatgagcagt ttttaaataa aaaataactt ggataacgat cgatttttga
    42361 tcattgtcca agtatttttt tggtatacta gtaccagtac catttctaag gagtgagtag
    42421 atgcggataa tatcaggaaa ttatggcggg cgccgtttaa aaagtttagc aggggcgaac
    42481 accagaccaa ctatagataa agtaaaagaa tccatattca atatgattgg accatatttt
    42541 gatggagaga cagtattgga tctgtttgca ggaagcggtg gtttagctat cgaagcagta
    42601 tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt accaagcgct aaaaatcatc
    42661 aaagaaaaca tcgaaattac gaaagaacca gagaaattta ccacaataaa acttgatgcg
    42721 gacaaagcaa tcgaacggct agcagcggag aaacaacaat tcgactactt gtttcttgat
    42781 ccgccatatg caaaacaaaa aataatcgat caaatccata aaatggaaga actgggactt
    42841 ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa cagtcgaatt gccagaaact
    42901 gtggctggat tccatcaaat aagaaaacag atttatggca taacggccgt aactatctat
    42961 agaaaagagg gaaatcaatg aaacgtgcat tattcccagg aagttttgat ccttttacaa
    43021 aaggtcactt agatacggtg gaacgtgcgg ctaaattatt cgatgaggta gtaattggtg
    43081 tttttatcaa tacttcaaag aaaagtttgt ttcccccaga agaaagaatg acattgatca
    43141 caaaagctgt ttctcatctt cctaatgtaa aagtcatgca tcaagaaaat caattgacgg
    43201 tagaaacagc aaaagagatt ggtgcagatg cgttgatcag aggcatacga agtatcaaag
    43261 actttgaata tgaacgagag attgctcaga tgaatcatca tctatatgat gaattggaaa
    43321 ctgtcttctt attagcaaaa ccagaatata gtcacgtgag ttccagtatt ttaaaagaag
    43381 tactgcactt tggcggagat gtgagcagct acttaccgcc agtgatcaac gaagctttag
    43441 ctggaaagcg tgaatcaaat gaaagctaaa caatggttga aaacagtact gattatcata
    43501 tttggaatgt tgatcattgg atttgtagta gttcccttgc cgtattatgt agaatcaccg
    43561 ggagccacga tcgatctgaa agaactaatt acggtcaatg ataaggaaga taaaggttct
    43621 gggtctttct ctcttacctc tgttggtatc aggcaagcta caggtttctt agctttaaaa
    43681 gctaaaatga ccccttttga agaattgatc agtgaagaag aattgactgg tggggcgaca
    43741 aatgaagaat ataatcaaat gcaacagtat tatatggatt cttcccaaaa tgcagcaatc
    43801 gaacaagcgt taaaacttgc agacaagcct tataaaatgg aattcaaagg cgtatatgtc
    43861 atgagtgtag agaaaacatc gaatttcttt ggcaagcttt cagttggaga tacagtaaca
    43921 aaagtagacg ggaaaagctt tcaaagtaca gaagcattta tggattatgt aaaaagccaa
    43981 aaagttggac aaacaatcga aatcagttat ttgcataatg gtgaagaaaa aacagcttct
    44041 ggtgatctga tcgagttacc aacagataaa aaggcaggaa taggtatcac gcttactgac
    44101 cataccgaga tcgaatcaga tacagatgtt cgtattaatt ctggctctat cggtgggcca
    44161 tcagcgggat tgatgttcac tttagaaatc tatgaacaag tcactggtaa aaacttgcga
    44221 catggtaagc aaattgcagg aactggaacg atcaacagtg aaggagaagt tggccgaatc
    44281 ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg gcgtagagat tttctttgca
    44341 ccagatgata cgatttcaga cgatgttaag aaagaatatc ctgaaataaa aaccaactac
    44401 gaagaagcaa aagctgccgc taaaaaattg aattcctcaa tgaaaatcgt accagtaaaa
    44461 acagtacaag aagcgcttga ctatctagag aaaatgaaat gaaactaaat aattaaagag
    44521 gggttgtgac acgagttctg tcacaacccc tctttttcgt caaaaatctt cacgataaaa
    44581 ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa gggacgattt tgccagagct
    44641 cactgtttct tcaattttaa atgaaaggag taatgaatga tttttactgg tcattctttg
    44701 aaaaaagaac aatgatagaa aaaatcagca agaaaatact agcttcaccc aaattgatca
    44761 tttggatgtc tatgatagtt cttatctttt gtatcgcaag tggtttttta ttatcaaatt
    44821 tgctatttta tagagaaaca gaagagactg ctgcaaaaga aatactttct tcgtttagtt
    44881 ccagtggaga agatccatca gctgaaggaa acgatgtaga agaggatgct cgtccaaagg
    44941 tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg tatctattcc ttttcttcag
    45001 aagaacgggt ctatgatgta ctaaagcgtg caggtggatt attagaagaa gcggatagcg
    45061 atcgaatcaa cttttctgcg aagatagaag atcagcaagt cctatatatc cctgctgtag
    45121 gtgaagagct gccagaacat cttgatcagt tggcatcctc ggaaggaaaa cagcctacag
    45181 cagattccga gcctcccaaa ataaacatca atacagccac tccatcagaa cttcagcaga
    45241 ttcctgggat cggatcagtc aaagcgcaag aaatcattcg atttagagaa gaaaacggat
    45301 ccttccagaa agtggaagac ctgcaggaga tttctggtat cggtgaaaag acagttgaaa
    45361 aactaaaaaa cttcgttaca attaaataga atgattcaaa atcgactggc acgagcaata
    45421 aatggacgct aatggatact aagaataatc caattctaag agcttggcag gacgaggagg
    45481 tagtattttt ggataggaaa tagcttgatt gttcgcaaag aaacattaaa aaaaccaatg
    45541 ctgaatgtct atctccaaaa taaaatatct gggattcata ttatgaatac ggctgtcagc
    45601 ggcaataaca gtcaagctct acgagaacgc tttgccaaag atgtacttag ctatacagca
    45661 gataaagtat ttattctcat tgggacgaat gatctagcag agcataaaca actttctaaa
    45721 gaaacgtacc aaaaaatctg cagtggatga tagttcagct gaaaaaagac tatagttctg
    45781 atcatgcttt ctttatcacg ccaccagcag tcgatgagac aaaacaaaaa tatcggaata
    45841 acaagttgat caaagaatat atctctgttc tcacagaagt agcaaaacgc catgattgtg
    45901 aagtatcgat ttttatacgg aatttcttga acaaatggat tcgaagaatt tttataatgg
    45961 gctaatggat gacggacttc atttcggcag tgaagggtag gatcttttgt cagacaggat
    46021 gattcgttat atttaaagaa tattttgttt aagcaatgag tactttttaa tcaacttgaa
    46081 aaaacggagg aatataaaac atgttagaaa aatcttcaaa agcatgcgat acgaataaag
    46141 aaaaagttac tgataagtca cataatgaac gcattccttg ggaccagtac tttatggctc
    46201 aggcagtact gttatcttta cgtagtacgt gtacacgttt agaagtagga gcgacactcg
    46261 ttcgagaaaa aaggatcatt gctggcggct ataatggagc agtcagtgga gatgtccact
    46321 gcatagatga agggtgttac gtcgtggatg gacactgctt gcgaacgatc catgcagaga
    46381 tgaatgcact cctacagtgt gcaaaacttg gtatcccgac ggaaggttca gaaatctatg
    46441 tcacacattt tccatgtttg gcctgtacaa aggctttgtt gcaagctggt gtgaaaggga
    46501 tcaattatct ccatgactat cgaaatgatc cttatgccca agcgttgatc gaacaattag
    46561 gtgtatctgt acaccaagtt gctttagact cacagcattt ttcaaaactt caaaatgaat
    46621 taacaggaaa acatgtctag cgttttagaa aggcatgttc gaaatcggtt gatttttccg
    46681 gcaattacgt cgatgattat ctgtttgttg tattacactg acaattgggg ttatcgtttg
    46741 cttcttgccg gaattttttt atggttttcg ctaaaagaag gacgttttat cgcgtacttg
    46801 tgtattttgt gtagcataac ggtatccatt tcttataggc tacaagagta ttcattttta
    46861 gaggaaaaaa caacatctgc gcaaattact gtatttacag atacgattcg tgcaaatggt
    46921 aattttttga caatggaagg aaggttatca gagaataaac aaaagctcca gctttcttat
    46981 caagtaaaca cagaagcaga attgaaaaca tggcttaact tccgaggaaa gggaatagaa
    47041 ttgactgctg aaggcgagtt catctctcca gcaaaaaagc ggaatcttta taattttgac
    47101 caagaagctt acttcaggaa ccatagaatt tctggacggt tccaaattca aaagattgaa
    47161 cagatcacca tgcaaaatca ctggcgaaac tggcttcaga gaaaaagagg atcttttatc
    47221 tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat atattaattc tttattatgg
    47281 ggctataaag atgcaggatt cagagaacca gaagaaattt ttagggagag tggtttgctg
    47341 catctgttca gtctatcggg tctacacatc caattttatt tagggtggct gtattattta
    47401 tttcgacgta tcggatggcc actacacgtc agtattattc cgttgagtct actaactatc
    47461 tgttatgttt ctattgcagg aagttctatt tctgttttgc gtgctagttt gctgttttta
    47521 ttgcgattgc tagtcaaatt gctgaataag cattattcca ccatggactt atttgcagtt
    47581 gtcttttggc ttcttttact atttgatcct cgatttcttt ttcatgcggg tggacagctt
    47641 agttttttca tgagtttttt gtttatcctg atttcttttg atggagattg gttgaaaaaa
    47701 acggcctctc acgtcctgtt tactatgatc acgatgccgt tgattgtatg gcatttttat
    47761 gagtggccta ttttaggtag tctttttaca ttactgattg caccggtatt caagtttctt
    47821 tttcttcctg gtgtgttatt tctcacactc ttcaatcgct ggctgccgga aactttactt
    47881 ttgctgatag aagagggatt aatcttattt gagcgaataa tcgatttttc ttcagggtcg
    47941 tctctcatta taggacgttt gccccttgtg ctatctggga tgtatatttt cggattactg
    48001 atttgtatgg ataggttgaa agaacaacct gtgaaacact tgttttatgt tggtcttttt
    48061 tcttttattt ttttgacttt gccattttta cgcttttctt caacgatcgc ttttatcgat
    48121 gtcggtcaag gagacagtat ctttttgcaa agtccttttc gaaaagaaac gattttgatc
    48181 gatactggag gtcagttaca ctttgaaaga gaaaagtggg cgcaaggtat gatacgtcct
    48241 cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg ggataagtgt aatcgataaa
    48301 ctcatcttga cacacgacga tacggatcat gtgggagaat tacccacgtt atctcagcat
    48361 tttactatca aaaaaattta tatcggttgg ggcgctggac gaaatgagcc attacaaagg
    48421 catttatctg aagctcaaaa aaatggtact gagatcattg aaatcaaaca tggggacagg
    48481 atcagcggtt attatgatct ttatgtattg actccttttg aaaagggaga gggaagaaat
    48541 gaagattcga ttggtctatg gatggaatat aacaataggc gctttttatt tttaggagat
    48601 ttgaatcaag caatggaaaa acaattgctt ttgatttatc ctaatctgaa agccgatgta
    48661 gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca cagattttct ttcccatatt
    48721 gaagcaaaac atgggattat atcctgcggc gtaaataatc gttatggtca cccgcacagc
    48781 gaagtattgg agacactaga agaaaatcag ataaaaacat ggcgaacgga tcaacaagga
    48841 atgatcacct atcgctggca tccagttttt catcctcatg ggctagtgga aacaatgata
    48901 gactagtaat tctcatctgc tcatggtagg ataatttttg aaagtggtga gtaatttgag
    48961 tatacaagca gagttacaaa aaatacgtaa agaaaaactt gcatcttgtt acttgatttt
    49021 aggaacagaa aagtttctcc aagatcaagt tcggacagaa attgtcaaaa aaatccaatt
    49081 agagggcgaa gatgacttaa attttttaag ttttgacatg gaaaatgcaa gtttagatga
    49141 ggtagtagct gaagcagaga cgttgccgtt tttcggagat caacggttag tattcgtaga
    49201 gaatccttat tttttaacag gtgaaaaagt aaacaatgga atcgaacaaa atactgattt
    49261 attgacggct tatttgaaag aaccattaga atcgactgta ttagtattct ttgcgccata
    49321 tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg aaaaaaacag caattatcat
    49381 caatgtccaa cagttgaatg aaaaagaagt acgtcaatat ctgatgaata ccttagagaa
    49441 ttcctcaatc aaaatggaac gaagtgcaat tgatttattc ttgaggttga ccgacttaga
    49501 tttatccaaa atgatgagag agttacagaa attggtcctt tatggacaaa atcaacagca
    49561 aatcacggta agagaagtag aagaattagt tcctaaaacg ttagaacata atatttttga
    49621 catgacccaa tatattttaa aagggaagac ggaacaagct ttgcgtttat acgaagattt
    49681 agttatgcaa ggggaagaaa caatcaaaat taatgcgatt ttgctttctc aattacgttt
    49741 atttttacag acaaaatttc ttgtaaagat tggttatcag caggcaaata ttgctgaaac
    49801 attgaaaatc catccatatc gtgtaaaact tgccatgcag gaagtaaaaa aattcgatga
    49861 acatttgctg gtacagttgt ttgatcaatt agttgagatg gattatcaaa tcaaaacggg
    49921 acaaatcgaa aaagaattaa gttttcaact attcgttttg cgcacagggc agttgttaag
    49981 aaatcgataa gaagaaagac gagggcaaag catgcgggaa ggtcgtttgt ttttgtcctt
    50041 tttttgacaa ataaaaaaat cggccgaagc cgattctttt atttagcgat ttttttgctt
    50101 aaacgagatt tgtcgcggtt agctttgttt ttgtggatta gaccttttga ttcagccata
    50161 tcaatagctt taacagcttc tttatataac gcatctacgt tgtcagcacc tgaagctaca
    50221 gcatcttcaa attttttgat tgcagtacgc atagcgcttg tttgagatga atttttcaca
    50281 ttagcgtttt cgcttgtacg aacacgtttg attgcagatt caatgtttgg cattagtttc
    50341 acctccgata acttaagttc ttacgcgtgt taccgcataa ttcaacattc attattatac
    50401 ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt ttttccttaa cagtctttta
    50461 ttaggatttt ttttcagtaa aaacggatag tacaagctga aaaaaatcaa aaagacgcta
    50521 ttgtgggagg caatagcgtc aaaccaaact agatttttat ttatctattt aaac
//
LOCUS       DAXWLG010000003        49267 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXWLG010000003 DAXWLG010000000
VERSION     DAXWLG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.003"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            115..486
                     /locus_tag="ACGE4T_000097"
     CDS             115..486
                     /locus_tag="ACGE4T_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFF8385417.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(760..1452)
                     /locus_tag="ACGE4T_000098"
     CDS             complement(760..1452)
                     /locus_tag="ACGE4T_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8385418.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1455..2240)
                     /locus_tag="ACGE4T_000099"
     CDS             complement(1455..2240)
                     /locus_tag="ACGE4T_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8385419.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2477..3877
                     /gene="lacG"
                     /locus_tag="ACGE4T_000100"
     CDS             2477..3877
                     /gene="lacG"
                     /locus_tag="ACGE4T_000100"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8385420.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3902..4219
                     /locus_tag="ACGE4T_000101"
     CDS             3902..4219
                     /locus_tag="ACGE4T_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8385421.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4232..5905
                     /locus_tag="ACGE4T_000102"
     CDS             4232..5905
                     /locus_tag="ACGE4T_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFF8385422.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5919..7550
                     /locus_tag="ACGE4T_000103"
     CDS             5919..7550
                     /locus_tag="ACGE4T_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFF8385423.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8041..9606)
                     /gene="guaA"
                     /locus_tag="ACGE4T_000104"
     CDS             complement(8041..9606)
                     /gene="guaA"
                     /locus_tag="ACGE4T_000104"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFF8385424.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9665..10588)
                     /gene="coaA"
                     /locus_tag="ACGE4T_000105"
     CDS             complement(9665..10588)
                     /gene="coaA"
                     /locus_tag="ACGE4T_000105"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFF8385425.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10784..11389
                     /locus_tag="ACGE4T_000106"
     CDS             10784..11389
                     /locus_tag="ACGE4T_000106"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8385426.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11697..12713
                     /locus_tag="ACGE4T_000107"
     CDS             11697..12713
                     /locus_tag="ACGE4T_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8385427.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12854..14155
                     /locus_tag="ACGE4T_000108"
     CDS             12854..14155
                     /locus_tag="ACGE4T_000108"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFF8385428.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14201..14863
                     /gene="deoC"
                     /locus_tag="ACGE4T_000109"
     CDS             14201..14863
                     /gene="deoC"
                     /locus_tag="ACGE4T_000109"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFF8385429.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14884..15282
                     /locus_tag="ACGE4T_000110"
     CDS             14884..15282
                     /locus_tag="ACGE4T_000110"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFF8385430.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15348..16430
                     /locus_tag="ACGE4T_000111"
     CDS             15348..16430
                     /locus_tag="ACGE4T_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8385431.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16642..18204
                     /locus_tag="ACGE4T_000112"
     CDS             16642..18204
                     /locus_tag="ACGE4T_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385432.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18197..19330
                     /locus_tag="ACGE4T_000113"
     CDS             18197..19330
                     /locus_tag="ACGE4T_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8385433.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19330..20286
                     /locus_tag="ACGE4T_000114"
     CDS             19330..20286
                     /locus_tag="ACGE4T_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8385434.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20385..21551
                     /gene="deoB"
                     /locus_tag="ACGE4T_000115"
     CDS             20385..21551
                     /gene="deoB"
                     /locus_tag="ACGE4T_000115"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFF8385435.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21575..22393
                     /locus_tag="ACGE4T_000116"
     CDS             21575..22393
                     /locus_tag="ACGE4T_000116"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8385436.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22410..23117
                     /gene="deoD"
                     /locus_tag="ACGE4T_000117"
     CDS             22410..23117
                     /gene="deoD"
                     /locus_tag="ACGE4T_000117"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8385437.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23415..24101)
                     /gene="gpmA"
                     /locus_tag="ACGE4T_000118"
     CDS             complement(23415..24101)
                     /gene="gpmA"
                     /locus_tag="ACGE4T_000118"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8385438.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24273..24854)
                     /locus_tag="ACGE4T_000119"
     CDS             complement(24273..24854)
                     /locus_tag="ACGE4T_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385439.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(24987..25670)
                     /gene="rpiA"
                     /locus_tag="ACGE4T_000120"
     CDS             complement(24987..25670)
                     /gene="rpiA"
                     /locus_tag="ACGE4T_000120"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFF8385440.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            25989..26402
                     /gene="rpsL"
                     /locus_tag="ACGE4T_000121"
     CDS             25989..26402
                     /gene="rpsL"
                     /locus_tag="ACGE4T_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFF8385441.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26486..26956
                     /gene="rpsG"
                     /locus_tag="ACGE4T_000122"
     CDS             26486..26956
                     /gene="rpsG"
                     /locus_tag="ACGE4T_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFF8385442.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27050..29137
                     /gene="fusA"
                     /locus_tag="ACGE4T_000123"
     CDS             27050..29137
                     /gene="fusA"
                     /locus_tag="ACGE4T_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFF8385443.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29309..30496
                     /gene="tuf"
                     /locus_tag="ACGE4T_000124"
     CDS             29309..30496
                     /gene="tuf"
                     /locus_tag="ACGE4T_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8385444.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30903..31211
                     /gene="rpsJ"
                     /locus_tag="ACGE4T_000125"
     CDS             30903..31211
                     /gene="rpsJ"
                     /locus_tag="ACGE4T_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFF8385445.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31242..31871
                     /gene="rplC"
                     /locus_tag="ACGE4T_000126"
     CDS             31242..31871
                     /gene="rplC"
                     /locus_tag="ACGE4T_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFF8385446.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31899..32522
                     /gene="rplD"
                     /locus_tag="ACGE4T_000127"
     CDS             31899..32522
                     /gene="rplD"
                     /locus_tag="ACGE4T_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFF8385447.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32522..32812
                     /gene="rplW"
                     /locus_tag="ACGE4T_000128"
     CDS             32522..32812
                     /gene="rplW"
                     /locus_tag="ACGE4T_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFF8385448.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32851..33684
                     /gene="rplB"
                     /locus_tag="ACGE4T_000129"
     CDS             32851..33684
                     /gene="rplB"
                     /locus_tag="ACGE4T_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFF8385449.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33727..34005
                     /gene="rpsS"
                     /locus_tag="ACGE4T_000130"
     CDS             33727..34005
                     /gene="rpsS"
                     /locus_tag="ACGE4T_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFF8385450.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34028..34375
                     /gene="rplV"
                     /locus_tag="ACGE4T_000131"
     CDS             34028..34375
                     /gene="rplV"
                     /locus_tag="ACGE4T_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFF8385451.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34389..35045
                     /gene="rpsC"
                     /locus_tag="ACGE4T_000132"
     CDS             34389..35045
                     /gene="rpsC"
                     /locus_tag="ACGE4T_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFF8385452.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35048..35482
                     /gene="rplP"
                     /locus_tag="ACGE4T_000133"
     CDS             35048..35482
                     /gene="rplP"
                     /locus_tag="ACGE4T_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFF8385453.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35472..35660
                     /gene="rpmC"
                     /locus_tag="ACGE4T_000134"
     CDS             35472..35660
                     /gene="rpmC"
                     /locus_tag="ACGE4T_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFF8385454.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35685..35951
                     /gene="rpsQ"
                     /locus_tag="ACGE4T_000135"
     CDS             35685..35951
                     /gene="rpsQ"
                     /locus_tag="ACGE4T_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFF8385455.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36009..36377
                     /gene="rplN"
                     /locus_tag="ACGE4T_000136"
     CDS             36009..36377
                     /gene="rplN"
                     /locus_tag="ACGE4T_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFF8385456.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36414..36722
                     /gene="rplX"
                     /locus_tag="ACGE4T_000137"
     CDS             36414..36722
                     /gene="rplX"
                     /locus_tag="ACGE4T_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFF8385457.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36750..37289
                     /gene="rplE"
                     /locus_tag="ACGE4T_000138"
     CDS             36750..37289
                     /gene="rplE"
                     /locus_tag="ACGE4T_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFF8385458.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37308..37493
                     /locus_tag="ACGE4T_000139"
     CDS             37308..37493
                     /locus_tag="ACGE4T_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFF8385459.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37529..37927
                     /gene="rpsH"
                     /locus_tag="ACGE4T_000140"
     CDS             37529..37927
                     /gene="rpsH"
                     /locus_tag="ACGE4T_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFF8385460.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37959..38495
                     /gene="rplF"
                     /locus_tag="ACGE4T_000141"
     CDS             37959..38495
                     /gene="rplF"
                     /locus_tag="ACGE4T_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFF8385461.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38656..39012
                     /gene="rplR"
                     /locus_tag="ACGE4T_000142"
     CDS             38656..39012
                     /gene="rplR"
                     /locus_tag="ACGE4T_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFF8385462.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39033..39533
                     /gene="rpsE"
                     /locus_tag="ACGE4T_000143"
     CDS             39033..39533
                     /gene="rpsE"
                     /locus_tag="ACGE4T_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFF8385463.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39548..39727
                     /gene="rpmD"
                     /locus_tag="ACGE4T_000144"
     CDS             39548..39727
                     /gene="rpmD"
                     /locus_tag="ACGE4T_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFF8385464.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39771..40211
                     /gene="rplO"
                     /locus_tag="ACGE4T_000145"
     CDS             39771..40211
                     /gene="rplO"
                     /locus_tag="ACGE4T_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFF8385465.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40211..41506
                     /gene="secY"
                     /locus_tag="ACGE4T_000146"
     CDS             40211..41506
                     /gene="secY"
                     /locus_tag="ACGE4T_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFF8385466.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41568..42215
                     /locus_tag="ACGE4T_000147"
     CDS             41568..42215
                     /locus_tag="ACGE4T_000147"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFF8385467.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42406..42624
                     /gene="infA"
                     /locus_tag="ACGE4T_000148"
     CDS             42406..42624
                     /gene="infA"
                     /locus_tag="ACGE4T_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFF8385468.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42657..42773
                     /gene="rpmJ"
                     /locus_tag="ACGE4T_000149"
     CDS             42657..42773
                     /gene="rpmJ"
                     /locus_tag="ACGE4T_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFF8385469.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42791..43156
                     /gene="rpsM"
                     /locus_tag="ACGE4T_000150"
     CDS             42791..43156
                     /gene="rpsM"
                     /locus_tag="ACGE4T_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFF8385470.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43184..43573
                     /gene="rpsK"
                     /locus_tag="ACGE4T_000151"
     CDS             43184..43573
                     /gene="rpsK"
                     /locus_tag="ACGE4T_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFF8385471.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43653..44591
                     /locus_tag="ACGE4T_000152"
     CDS             43653..44591
                     /locus_tag="ACGE4T_000152"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFF8385472.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44619..44999
                     /gene="rplQ"
                     /locus_tag="ACGE4T_000153"
     CDS             44619..44999
                     /gene="rplQ"
                     /locus_tag="ACGE4T_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFF8385473.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45271..46110
                     /locus_tag="ACGE4T_000154"
     CDS             45271..46110
                     /locus_tag="ACGE4T_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8385474.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46086..46955
                     /locus_tag="ACGE4T_000155"
     CDS             46086..46955
                     /locus_tag="ACGE4T_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8385475.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46945..47742
                     /locus_tag="ACGE4T_000156"
     CDS             46945..47742
                     /locus_tag="ACGE4T_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8385476.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47945..48691
                     /gene="truA"
                     /locus_tag="ACGE4T_000157"
     CDS             47945..48691
                     /gene="truA"
                     /locus_tag="ACGE4T_000157"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8385477.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49020..>49267)
                     /locus_tag="ACGE4T_000158"
     CDS             complement(49020..>49267)
                     /locus_tag="ACGE4T_000158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385478.1"
                     /translation="KMTNLFRLSSLRAIKTTFHRFLVTEKIGNKKMIHLSTPRPQVTI
                     FLKDEKLFFEIVTCDSELNDRLTTSFLNGVQSADLRY"
BASE COUNT        15598 a         8994 c        10807 g        13868 t
ORIGIN      
        1 ttaatagatt taataagtac tgataatgaa atttctcctg gttgggaaaa cgaagttggt
       61 taccctgtag aagaattttt tcaagattac tttgcggagt atgccgatag gataatgaga
      121 gattacgtaa cagataaaac tcaagaagtc atcaatcaat cccagtcatt aaatgaatta
      181 tctgataagg cgaaagaaga agcagttgcc attatttctt cagggattag tgactatatt
      241 gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa ttgtacgcat gataaaagct
      301 tttatcaaaa cttttgatgt gaaggcagat cttaagttta atgatactta cctagtagga
      361 aaattaatta atatccttgg gacagagaaa gatacggaaa cgtttatttc ctctttgtct
      421 atgttgttta cagataagca tttttcaact cagcttggtg gagaggaact agtggaaatc
      481 ttttaaccat tacgcccagc attaataaaa gtatatcaaa aaagtactcc tgatgatttt
      541 taagattggt ttgagaaata atgtgaaaac actattttta atgttaatta tagatagaga
      601 tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga tacactgcta tatttcacta
      661 aggatatagc agtgtatctt tcatgtcgtt tcactggcag ttttttataa aaggaggtgt
      721 gcatttcagt tgcttttctt tggtagttag ttgaatcgtt taagaattaa cggatgtggc
      781 agacaggtgc tctgtccaaa aaccagtaaa atctttgatg gttccatctt tatttagctg
      841 tgcctgttca aatactgttt tgtttttacc gaatggatca attgcaataa ttgttccgat
      901 ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct tcgataacaa gacaatcagc
      961 cggagctaag ctcagtttgt ctgccgcttt aagaaaaata tccggttgcg gtttcccagg
     1021 gaaagagccg tcgtcataga caatcttgtc ccagtcaaac cagcgggcta aatcgaaaat
     1081 atcaaaataa aaagcaacat tctctttgac agtagccgtg gcaatcgtta atggaatctg
     1141 ttggtcagtc agctggtcaa gagtcgcttc aagccctttt gtcaactgaa gctcgttttc
     1201 attttgcaag caaagcatac gataatagct ttctttttct tctgacaata cggcgatctc
     1261 agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc tccgtattcg tccgaccatg
     1321 aatatttttt aaaatatcct cgtctgataa accatctttt gtatgatcat gtatcatatg
     1381 cagccaagca gcttcatgca gatgagagtc caaaaacatt gttccgttga aatcaaatat
     1441 aatccctttc atagttacgc tcctatagtg gtattttcgg ttgaatcagc aatttgcaga
     1501 tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga tctggcgatc cgtaatgagc
     1561 agatcgaaat catgcaaatt ataaaacaca taaaaatcat ctcgattgaa ttttttatta
     1621 tcaacgagta gatatttctt acgggaatta tttagagcga tctgctgtgc ttctccttct
     1681 tcggtactgt aagtagagat cgcttcgttg tgaacgccat ttgaactgat aaatgctttt
     1741 gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc cgacaaatgc gccggtattt
     1801 ttccggaaat caccgccaat cagtagaatt tctggtccat ctgcttgagc aagaatatca
     1861 aaaactggat aattatttgt aatgatccga attttcttgt tcagtaaatg cgtcgccaat
     1921 agttcgattg ttgtacctgg tcctaagaaa atcgtttctc cctcgtgaat caaggaggct
     1981 gctgtggcag caatttcttt tttttcttga atctgcagag tggctttttc agtgtgagaa
     2041 agttcttgat ctaaagtgta ggaaagactt tgagcccctc catggacacg cagcagcttg
     2101 cccgattttt ctaattcatc taaatctctg cgaacggtca tatcagaaac atctaattga
     2161 ttgatgattt cattaacggt aaggattcct tttttattga ccatttctac gatagtaagc
     2221 aatctttctt ttttcaacat tgtcttcacc tctaatttaa ggataatcta tcatataaac
     2281 ggacaacagt caatctaatt cgaacaaaag taaacattta gggtggtttt tatgtttgtt
     2341 acaatagatt ttattgctgt tttctggttt ttatgattat ttgtgtttgt ttttgttgac
     2401 aaatgttttg gatgcgttta caatgcaatc agttagaaag agcgaaaact gattatagat
     2461 ttgaggagag atgtacatgc ttaaattacc agaagatttt atttttggcg gagccacagc
     2521 agcctatcag gttgaaggag ccactaagga aggtggcaaa ggcgctgttg cttgggatga
     2581 ctttttagag gaacaagggc gattcagtcc cgatccggca agtgattttt atcatcaata
     2641 tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat ggtctaagat tatccattgc
     2701 ttggagccgc atttttcctg atggagctgg gaaaccaaat ccagaaggaa tcgcttttta
     2761 tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg ccgtttgtga cgttgcatca
     2821 ttttgatacc cctaaacgtt tgtttgacca tggagatttt ttgaatcgag aaacaataga
     2881 agcatttgtt tcctatgcca ttttttgttt tcatgaattc aaagaagtca aggtgtggag
     2941 tacctttaat gaaatctatc cagttgcaac caatcaatat ttgttaggtg ttttcccacc
     3001 aggaatcaaa tatgatttta caaagatcgt cgcctgtctt cataatatga tggttgctca
     3061 tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg ggtgaaattg gtgtagttca
     3121 ctcattagag acgaaatatg cggcaacaga cgcgccggaa gataagcatg ctgccttttt
     3181 agacgatgca ttgtcgatcc gtttcttatt agatgctacc tatttaggct attattcaac
     3241 tgaaaccttg actgctcttg atgagatatg tgaagcaaat caggccagct atcattttcc
     3301 tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt aatgactact tagggatcaa
     3361 tcactaccaa tgccattttg tcaaagctta cgatggagaa aatgcaatcc atcataacgg
     3421 tacgggtgaa aaagggacct ctgtttataa agtgaaggga atcggagaac gtatttataa
     3481 agaaggaatt cctcgaactg attgggactg gctgatttat ccagaaggtc tatatgactt
     3541 gttgctgcga attaaaagcg attacccaca ttacaacaaa atctatatca ctgagaacgg
     3601 gatggggtat aaagatcagt ttgaagatgg cattattatg gatcagccga gaatcgacta
     3661 tttgagagtc tatttggaat ccttaagcaa ggcgataaca gccggggtaa atgtgaaggg
     3721 gtattttttg tggtcgctta tggatctttt ctcatggaca aatgggtata acaaaaggta
     3781 tggcttgttt tatgttgatt ttgaaaccca gaagcgttac ccaaaagaat cagcttactg
     3841 gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa gtgataatgg aggaagaaaa
     3901 gatgaataaa gaggaattac aaatgcttgg ttttgagatc gttgcatatt caggcgacgc
     3961 tcgcagtaca ttattgaaat tattgaaaga agtaagacaa ggaaactttg aacatgttga
     4021 ttcggcgttg aaagaagccg acgaaaattt aacccttgca cataattcgc aaacaaaaat
     4081 tttagcggaa gaagcatctg ggaaagagat ggagatggga ttcattttta tccatgggca
     4141 ggatcattta atgaccactt tattgctgag agatctgatt caagacttta ttgtactgta
     4201 cagacagaat caaggatagg agaagaacgc tatggatgga atcattaaac aaatcgaaaa
     4261 agggaaacca ttttttgaaa aaatctcacg taatagttat ttaggagccg ttcgtgacgg
     4321 atttttaaca gcgatgccag ctattttatt ttcaagcatt ttcattttga ttgctgcggt
     4381 tcctgagatt tttggttacc aatggcctga atctgtctcc acatggctat ggaaagtcta
     4441 taattattcg atgggtgttg tagggttact cgtttcagca accactgctc gctgcttggc
     4501 tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc aacaccacct ctgtgatgct
     4561 ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg attgaaggtg gattttcaac
     4621 ggaatatatg gggacaaagg gactactggc ttcctttgtc gctgcatttt taacagttaa
     4681 tttatataag ttttgtgtgc tgaaagatat taccattaaa atgcctaaag aagttcctgg
     4741 aacgatttcg caaaccttcc gagatatttt tcctttctct ttttcagtat ttgcggccgt
     4801 tttaattgat ttaggcattc gacaaatctt ccaaatctca tttgcagaag caatcattac
     4861 ggtgctgcag ccgctattta cagcagctga cgggtactta ggaatcgcta ttatctgggg
     4921 agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg tcgatcgtgg agccggcaat
     4981 tgcggcaatt atctatgcca atgtggaaac caatctgcaa ttgttcaatt ccggtgaaca
     5041 agcttctaaa gtactgacgg tgggtttagg gaattttgta ggaactatgg gaggaactgg
     5101 ggcaacattg gttgttcctt ttatcttcct actctttgct aagtctaagc agttgaaggc
     5161 agttgggaaa gcttccttta ttccagtaag tttcgctgtc aacgaaccat tgctatttgc
     5221 ggcacccatg attttgaacc cttatttttt tattccattt ttgttaacac ctgttgtaaa
     5281 cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg aacagcttta tgtacgtttt
     5341 gccttgggca actccagcac cgatcggttt ggttctagga acgggcatgg gtatcttagc
     5401 ttttgttcta gcagcggttc taatcgtcgt tgacttcctg atctatctac cattttgcaa
     5461 agcatacgat gctgtcttag tagagaaaga agcacaaaaa gcagaggtga tcgcaaccga
     5521 ggaaggtgct tctgccgatg tacgtgagga agtacctgtg gcagcgagtc aagttatcag
     5581 cgatgcaaat aaagaaatca atgtgttggt actttgtgct ggcgcaggga caagtgcgat
     5641 gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa gcaccgatca gtgcaatggc
     5701 gggtgcttat ggttctcatt acgaaatgct gcaggatttt gatatggtgg tcttagcacc
     5761 gcaagtcaat tcttactatg atgatatcaa agaagacacc gatcgcttag gaatcaagct
     5821 ggttgcgaca aaaggagcag aatatatcgg attaacgaga gatcctgaaa aagctgttca
     5881 gtttattctg aaccaatttc aataaaggag gagccataat gaaaatggga atttggttgc
     5941 gtaaagctgt tttatttgca gctgtgttag gggctttggg ggctgtttca aaaactgttg
     6001 aagcatctgc cagcgaattt attcgcggag cagatatttc gatcctgtca gatatggaaa
     6061 aatctggtgc aaaatattat gaagacgata tttcaaaaga tgccttagag attttaaaaa
     6121 ataatggtgt caactatgtt cgactgcgtt tgtggcagga tccttataat agtaatggag
     6181 aaagttatgg agcagggaca aatgatctgg aaacgacgat tgaccttgca aaacgagcaa
     6241 aaaatctggg acttaaggtg ctgctggatt ttcactacag tgatttttgg gttgatcctg
     6301 ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt tgaagaaatg aatacggcat
     6361 tgtacgacta tacaaagaac gttctgtcgg aaatgaagca gctggacgtt tatcctgata
     6421 tggttcagat cggcaatgaa ctgaatagcg gaatgctctg gccttatggc aagtcttggg
     6481 gagaaggcgg gggagaattt gatcgattgg ctgctttttt gaagtctggt atccaagctg
     6541 ttcgagacac tcaacctaaa acaactccag taatgctcca cttagctgat ggtggcgata
     6601 caggagcatt tacttggtgg tttgatgaaa tcacgagtcg aggtgtgtca tttgatttaa
     6661 ttggagtgtc gtattatccg tattggcacg gttcaatgaa tgaacttcaa accaatatga
     6721 atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt tgaaacagct tatgggcata
     6781 caacagcgaa tgccgatact atgccaaatg cttttggtga agccgaagca gctgcggggg
     6841 gatatgaacc aacaccgaca ggacaagcag agtatttgct ggatcttgcc gatcggattc
     6901 aggcagtacc gaataatcgc ggggcgggct tcttctattg ggaacctctt tggtacaacg
     6961 gaaatgtgag ttgggcaaca caagcaggta tgaattatct cagtgttcaa tccactatgg
     7021 gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg aaacgccttg ccaagcttgc
     7081 gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa aaatgcaagt ttcgaaagcg
     7141 atggaccaac gaccagtcct tccaattggc aaacctggtt tcaacaagga accgctaagg
     7201 gtacgatcaa aacggaagcg ggcacttttt ttggggacta caaactaact ttttggcagg
     7261 atcaagcata cgaggcatca gcttatcaaa atgttagtgg cataagcaaa ggaacttaca
     7321 cattgagtgc ctatgtaatg tctgggggag agtttgatac taatcagtta tatatcaaaa
     7381 attatggtgg agcggaacta aatactgcgg ttgttaaaac aagtgaatgg acaaaaatta
     7441 caatcaaaga cattccaatt acaacaggtg tttgtgaaat tggggtttat agcaaggctt
     7501 caggaaatgc ttggacgaac gttgattttg tttcattggt gaaacaatag actgcggatc
     7561 gtttcgattc aagatcggag gttcccttca gccacatcgt tagcgtagaa ggcaggaatc
     7621 gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga tttccagtca tttttaccaa
     7681 aacgagtaaa gcaccagact tcatctacac tttgtattat gctaaagaga gttcaacgat
     7741 ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag ataacataaa gtttgtttaa
     7801 gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga gcacttagat ggatagaaaa
     7861 aagttctcgg aaaacagttc gtttttcgag aacttctttt tatgataaga atgaaacaaa
     7921 ttaacttata ttatagtctc ttttggtagg gaaccatctt atttttaatg aaaatcaagc
     7981 attttagcta ttgaagaatg ttgatttata gggattcccc ctccatatta tgttggctct
     8041 ttattcccac tccactgttg ctggtggttt agaagtgatg tcatacacga tacggttgac
     8101 gtgggctact tcgtttacga tacgtacaga gattttttgc aagacttccc atgggatacg
     8161 ggcaaagtca gcggtcattc cgtcgattga agttacagca cggataccga ctgtgtagtc
     8221 gtaagtacgt ccgtcaccca taacaccgac agagcggatc cctggaagaa cagtgaaata
     8281 ttgccagata tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga tagcatcaga
     8341 ttcgcgtacg atttctagct tgtcttcagt gatttcgcca agaacacgaa taccaagacc
     8401 aggaccaggg aatggctgac gccaaacgat tgaatcaggc atgcctaact gagtccctaa
     8461 ttcacggact tcgtctttga ataatgtgtt taatggttcg atcaattcaa attgcatatc
     8521 ttctggcagt ccacctacat tatggtgaga tttgatcgtc tgtgctgttt ctgtaccaga
     8581 ttcgataacg tcagtataca acgttccttg tgctaggaaa gagacgcctt cttcaccagc
     8641 aagttttgtt gcttcgtcat caaaaacata tacgaattca ttcccaatga ttttacgttt
     8701 ttgttctggg tcagatacac ctttcaactt gtctaagaag cgatctcttg cattgacttt
     8761 gatgatgttc aagccgaatt ttccgcctag actttccatt acttgttccg cttcgccttt
     8821 gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg ctttttgtaa
     8881 aagaacccca acgacactag aatcaacgcc acctgaaaga ccaagcaaga cttttttgtc
     8941 accaacttgt tcacggattt tttctacttg catatcgata aagttatcca ttgtccagtt
     9001 accttcacag tgacaaacat caaaggcaaa gtggcgcagc aattcgattc cgtattcgga
     9061 atgacgtact tctgcatgga attggatacc ataaaaatta cgtgttgtgt cttggataga
     9121 agcaatcgga caatctttgc ttgttccgac tgtttcaaag ccttcaggaa ctttagttac
     9181 caagtcacca tggctcatcc atactgtttg ttgttttggt gtttctttaa acaagacagc
     9241 ttgatcgctt gttacttcta acaaggcttt cccatattcg cggtttttag caggctctac
     9301 ggaaccgcca agtttgtacg tgatcaattg cataccatag caaatcccta atacaggaat
     9361 acctaaattg aagatctcag gatcgatccc aaatgcattc tcatcatata cactgtttgg
     9421 tccgccagaa agaatgattc cctttggatt catcgctttt acttcttctg cagtaatgcg
     9481 gtggctcaat aattcggaaa agactccaag atcacgaata cgtcttgtga tcaattggtt
     9541 atactgacta ccgtagtcta atacgataat cttttcaacg ttcgtcatat cggcaacgtt
     9601 tgtcaaattt attaccccta tccctttaaa ttttatctca aaatagtcaa actattttaa
     9661 aatgctaata ttttcgcatg aaaatcgaat cgatgatatg attttcagtt ttatggagaa
     9721 ttatatcggc tcttcctcgt gtaggcaaga tatattcctg taaatttttc aaattcacat
     9781 ttttccatac atcttttgcc attttaaagg cttcttcacg atctccgata gcaaaacgat
     9841 aataataatt ctctggattt tggaaagccg tatccagtaa cgcaccaaaa cgatccaaat
     9901 accatttttc gatcagcacg ggatcggcat cgacatagac agaaaaatca aaaaagtcgc
     9961 tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt gatcccctca acgatcaaga
    10021 tatcaggctg ctggatcaat tcgtacttgt ctggaatgat atcatacaca tcatgtgagt
    10081 aaacaggtac ttttatctct tcctgaccag attttacacg gttcaagaaa tcgatcagca
    10141 tctccatatc ataactttca ggaaatcctt tacggtccat gattccacgc tcttttaata
    10201 tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt cgtttgaagg
    10261 tacgagacaa aatcgtctgc agcagtctgg ctgttgtact ttttccaacg gctacacttc
    10321 cggcgatccc gataataaaa ggcggtactt ctacgtattt atgaagaaat aaacctttac
    10381 tcaagaacaa agattcaaat tctttcatat aaagataaat cagatgagca agaggtacat
    10441 agatatctcg aacatcctgc atggaaatgc ggtcattcaa acttttgata ttatctagtt
    10501 catcttgtgt caaaggtgct tgcccattac gataaaattc tcgccattct tctcttgcca
    10561 cacggtagta gttcattgga tcattcatat tagcctcctg cgttctccca tcaatggaac
    10621 tattttaaca caaaataaac gcaaacccca ccacttaaga gagattgccg atctttttgt
    10681 ataatcttta taatagaaag aaacggagtc ttattcttga cttgcgcacg gatacataaa
    10741 aggatagact gttgtcatga aagaaatgag aaggatggaa gacatgacaa atcattacta
    10801 ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg gcttttgagt taaaaggcag
    10861 aacctttcaa tttgtaacag attctggtgt attttcacgt gaaacagtgg attatggttc
    10921 acgtgtcctg attgatgctt tcgattggga agagttacct gatgaaggaa agatcttaga
    10981 tgtgggatgc gggtatggac cgattgggtt agctttggca tttgcgacac agcggtttgt
    11041 tgaaatggtc gacattaatg aacgagcggt aagtctagcg caaggaaacg caaagcgtaa
    11101 ccaaatcgaa catgttgata tccatcaatc gaatatctat gaagcagttc atgaagaaac
    11161 ctatgcagct atcgtaagca atccgccgat ccgtgctgga aaaaaagttg tacatgaaat
    11221 tttgacaggc gcatatcctc ggttgaagaa gggcggtaca ctgacaattg tgatccagaa
    11281 gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa acatttggta atgcagaaat
    11341 cgtcacaaaa gataaaggat attatatcat caaaagtgtg aaagaataac tagctgaaaa
    11401 atagtggagc gacgaaagag tggaactggg cgtgcatgta cgggtacgta tcagttcttt
    11461 ttttgttgag aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga aatagtagga
    11521 acagagtcgt tgttttttac gataaaataa ataagataca gaacttgtat agaaggctgc
    11581 caagtcggta gataaaggag gtttttatca aaaaacatac tttccttttt tctctattct
    11641 tttcttgggg gttttggtac actgataggg aatttagata gaagggagcc aataggatga
    11701 agctgaccat cagagatatc gcagaaatgg caggcgtatc agtaacaact gtctcacaga
    11761 ttttgaataa caaaggaagc agatttagtg ataagacaag aaaaaaagtg ttgtcgatcg
    11821 tgaatgaaca tcattataag cccgattatt tcgcttcaaa tgtgatcaat cgacattcaa
    11881 agaccatcgg aatgatcgtc cctgatgtca ctgatttctt cttctccaaa ctgatcgaag
    11941 gtgtagagag ctatttgaat cctttgggct atgtgatcat gttatgcaat tctcgtcata
    12001 gccaagaaaa tgagatcaag tatttacaag agctttccca ccgctcagtg gacggaatct
    12061 tgctggccac accaaatatt ttacctgaga aatatgcatt agacagcggt ttttatcaaa
    12121 aaatgccaat cattttaatc gatcgcggat tgaatcgtcg ggataacgga cggcttatcg
    12181 taaaggaata tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat ggccatacga
    12241 agatcggcat gctgaaggaa accactggat actatcaatt agaagaacgt ttcaacggat
    12301 atcgtcatgc gctgaaagat catggcctgc cttttaatgg gaaatttgtt gaacatggag
    12361 agttgactgt ccaaggtgga tatactgcat caaaaaaatt actcaaacat aaagatatca
    12421 cggctatttt ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg atcgatgaat
    12481 tgggcaaaaa aatcccagaa gatatctcag tcataggatt cgatggatta aagctgtcag
    12541 aatacatgat tccgcaattg acgacagtgc agcagccaag ttttgatatt ggattttatg
    12601 ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag aaaagttccg aacaaagtgt
    12661 tcgaaacaaa attaattatt cgtgagagcg taaaagtatt gacaaagggc taactcggcg
    12721 gtatattgct tatgtaagca tttacaaagg ctagttagtg ctttttttat ttttagtaaa
    12781 ccagtttact aaagtggttt acctttccta aaaataatgt ataataccga atgaagacaa
    12841 ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa aagagggatg gaaacgagct
    12901 gtcgaaagag gaaatcgagt acatcgtcac gaactatacg aatggaaaga ttccagatta
    12961 tcaagtcagt gcattattga tggcaatttt ttatcaagac atgacaaatg aagaaatcac
    13021 gaacttaaca ttggcgattg ctaattctgg tgatgtcatc gatttgagtt ctcttgaagg
    13081 aataaaggta gataagcatt cgacaggtgg cgttggtgac acgaccacat tgattttagc
    13141 ccccctagta gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg gtctaggtta
    13201 cacaggtggt acactagaca agctagaagc aattcccgga tttcagatcg agctttcgga
    13261 tgaagaattt gtacgcattg ttaatgaaag taaagtagcg gtaattggtc aatcaggaaa
    13321 cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga ccgttgattc
    13381 tcttccgctg attgccagtt cgattatgag taaaaaaatc gcggcaggag cagatgcaat
    13441 cgttttggat gtgaccactg gagacggtgc tttcatgaag aacatcgaag atgcaagacg
    13501 tctggcaaag accatgacga gcattgggaa actggctaac cgagaaacag tggcggttat
    13561 ctcagatatg tcagaacctc ttggcgaagc tatcggaaat agtctagaag ttgtcgaggc
    13621 aatcgaaaca ttacaaggaa atggtccaga agatctagta gaaatgtgct acgccttagg
    13681 aagccaaatg gttgttcttg ctggcaaagc taagacaacc gatgaagcaa gaacgttgct
    13741 tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga tccaaaatca
    13801 agggggagac ccagcgatcg tggaacatcc agaacgaatt ttgactgcac gctacacaat
    13861 ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg aattaggaat
    13921 tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg atcacgctgt
    13981 tggattgaaa ctgcataaaa aaattggtga cactgtgaca aaaggtgagt cactattgac
    14041 gatctatagt aacgacgaag aaatcacttc tgtcattgaa ttattatata aaaatattga
    14101 aattggtgaa tcggcaatga agccaacgtt gattcatgac atcatcacag aataaaggaa
    14161 aaaagcggac tttgccgttt taaagaaaag gggaaaagaa atggaactaa accgaatgat
    14221 cgaccatact attttgaaag cagacgcatc taaagaagct gtgatgcaaa ttatcgaaga
    14281 agcaaaaaaa taccattttt attctgtctg catcaatcca gcatgggttg cgctagcgaa
    14341 agaacaatta caaggtgagc cagtagccgt atgtacagta atcgggtttc ctctaggagc
    14401 aaacacaccc gagacaaaag catttgaaac aacagatgcc atcaataacg gtgcagatga
    14461 agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac aagtccaaaa
    14521 agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag ttattattga
    14581 aacatcgtta ttagatagag aagaaatcat ccgagcttgc gaaatagcaa aagctgcagg
    14641 cgctgatttt gtcaaaacat caactggttt ctcaacaggc ggagcaaagg tagaggatgt
    14701 ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc aaagcttccg gcggtatcca
    14761 taacgaagaa gaagcaatgg caatgatcga agctggtgct acaagaatcg gaacaagcgc
    14821 aggagtagca atcgtatctg gttcaactgg tgaaggctat taattttttc aaaggagaag
    14881 ctcatggcag aactcaaaca agaatggatc aacacagcta ttgaagcgtt agataaagcc
    14941 tatgttcctt attcccattt tccggtaggc gcttgtctcg taacggaatc aggcaagatt
    15001 tatcaaggca tcaacattga gaatgcatca tttggattaa cgaattgtgc cgagcgcaca
    15061 gcttttttca aagcagtatc tgaaggagaa aaaacattta cgcatctagt cgtagcgggg
    15121 cacacccccg atcctatctc tccttgtggt gcatgtcgac aagtaatggc agaattctgt
    15181 tcccctgata tgccggttac gctagtagga gacaatggtg tgacgaaggc aacgaccgtt
    15241 cgtgcactgc tgccttatgc atttactgaa aaagatctat aactcatgtt cattcagtca
    15301 gctgactgtt gaaaaataaa acaattattt taggaggctt tacagaaatg aaaaaagcaa
    15361 aattatttgg tttaggtgct gtcgcactag cagcagggtt atttttaggt gcatgcggca
    15421 acaacggctc tactgattca tccggtggca aaagctcatc agacacaaca actgcagcat
    15481 tgatcacaga cacaggtggt gtagacgacc gttcattcaa ccagtcagct tgggaaggac
    15541 tagaaaaatg ggggaaagat caaggacttt cacgaggaaa tgatggcttc caatatttcc
    15601 aatcttcaaa tgaatcagat tacatcccaa atattgacca agcattgaat gctggattca
    15661 aaacgatttt tggtattggt tataaactaa aaccagcgat ccaagaacaa gcaacaaaca
    15721 atacaggtac gaactttgtt attatcgatg atgtcatcga tggattagat aacgttgtat
    15781 ctgcaacttt caaagacaac gaagcttctt atcttgcagg tgttgcagca gcttatacga
    15841 ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc gatcgttttg
    15901 atgctggatt caaagccggt gttgatgcag gtgcgaaaga attaggaaaa gaaatcaaag
    15961 tattgaatca atatgctggt gacttctctg ctccagataa aggacgttca attgctcaag
    16021 gtatgtatgc acaaaacgca gatatcatct tccatgcttc aggcggtaca ggtaacggtg
    16081 tattccaaga agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg gttatcggtg
    16141 ttgaccgcga ccaatcagac gaaggcgaat acacattaaa cggcgaaaag aaaaacttca
    16201 cattgacatc tacactgaaa gcagttggaa cagtagtaga agatttggca caaaaatcag
    16261 cggacggtaa attccctggc ggcgaacaca ctgtttatgg attgaaagaa gacggtgttg
    16321 gtctaacaga aggacaactt tctgacgaag cgaaaaaagc agttgatgaa gcaaaagaaa
    16381 aaatcatttc aggtgacgta aaagttccag aaactcctga agaaaactaa aaccatttct
    16441 atagccttgt tagacacatg tttgacaagg ctatggtttg attagaacaa gaattgttca
    16501 tttatccaag ctgccttgtg acagtttgga taagggaaca actccctgaa gtaatgtttg
    16561 tttcaaaaga agaacgctca ctaagcgtga atcttagtca atcgttgccc tagatagaaa
    16621 tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat gcagaatatt
    16681 accaaagaat ttggaacatt caaggcaaat gacaacatca atttacaagt aaaggcagga
    16741 gaaattcatg cgttgcttgg agaaaacggt gctggcaaat ctacattgat gaacgtgctt
    16801 tccggattat tagagccgac atcagggaaa attttgatgc gtgggaaaga agtaaagatc
    16861 acaagtccga ccaaagccaa ccaattaggg attgggatgg tccatcagca ctttatgctt
    16921 gttgatgcct ttactgtaac agaaaacatc gtgttgggaa gcgagcctag tcgcgcaggg
    16981 atgcttgacc ataaaaaagc acgaaaagag atccaaaaag tttctgaaca gtatggattg
    17041 tcagtcaacc cagatgctta cgttcgtgat atttcagttg gaatggaaca acgggtagaa
    17101 attttaaaaa cactttaccg aggagcagat gtactgattt ttgatgagcc gacagctgta
    17161 ttgacccctc aagaaattga tgaattaatc gtgatcatga aggaattagt caaagaaggc
    17221 aagtcgatca ttttgattac gcataagtta gatgaaatca aagcagtagc tgaccgctgt
    17281 acagttatcc gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt aacctcacaa
    17341 caattagccg atatgatggt tggaagaacg gtttcattca aaacgatgaa aaaagaagcg
    17401 aagcctcaag aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa tcgtggatta
    17461 gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg tatcgctgga
    17521 atcgatggaa acgggcagtc ggagttgatc caagctttga ctggtttgcg aaaggcagaa
    17581 agcggacata tcaagctaaa aggggaagac atcactaata aaaaaccccg aaaaatcact
    17641 gaacatggtg taggacacgt gccagaagac cgtcataaat acgggttagt cctagatatg
    17701 acattatctg aaaatatcgc actacaaacc tatcatcaaa aaccttacag taaaaacggc
    17761 atgctgaatt attcagtaat gaatgaacat gccagagaat tgatcgaaga atatgatgtt
    17821 cgaacaacga atgaacttgt tcccgcaaaa gctttatcag gcggaaatca gcaaaaagca
    17881 attatcgctc gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa tccaactcgt
    17941 ggactggatg taggtgcgat cgaatttatt cataaacgtc tgatcgaaca aagggacaaa
    18001 tacaaagcag tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt ttcggatcgt
    18061 attgctgtta tccatgaagg agaaattgtc ggtatcgttg atccgaaaga aacatctgaa
    18121 aatgaattag gtctattgat ggcgggctat accttagaag aagcacgaaa agagttagaa
    18181 aagggggctg gcgcacatga atgatcgcag cgaaaaattc cgtaatttat tagtgcctgt
    18241 actttcagtt gttttaggat tgatcttagg cgcgatattg atggctgctt ttggttttca
    18301 gcccatccaa ggttattctt ctatgctaga tgcagccctt ggttcgcaaa gagggatagg
    18361 tgaaacgtta aggcaagcaa cgccgttgat ttttacagca ttaggttttt ctgtagctaa
    18421 ctcagctggg tttttcaata ttgggctttc tggacaagca ctttgcgggt ggatcgtcag
    18481 tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt gttttgcttc cgttatgcgt
    18541 gatcttaggt gccttagctg gtgcgatggc agcggctgtt ccaggtttac ttcgagcatt
    18601 ttttggaaca agtgaagtga tcgtaacaat catgatgaac tatatcttat tatacgtaag
    18661 cacatttgct ttacatgaag tgatgcctga aagctatcgc tcaagtttgg actcttccaa
    18721 tatgatcagt caaaatgcct cactgcggtt accatggtta acacaagctt ttggcggctc
    18781 tcgcgtaaac tcaggattat ttttagcaat catcgcgctt attttcgttt ggctgctgat
    18841 gaagaaaacg actttaggat ttgaaatccg ttctgttgga ttgaatcctt ttgcttctga
    18901 gtatgcagga atgagtagta aacgtacgat cgttttgtcg atggtcattt ctggcgcttt
    18961 ggcagggctt ggcggcgtgg ttgaaggatt aggaacgtat caaaacttct ttgttcaaac
    19021 gacttcgcta tctattggat ttgacggaat ggctgtttcc ttgcttggtt ctggatcagc
    19081 aatcggtatt cttttatcag cgatcttgtt cagtattttg aagatcggtg gacttggcat
    19141 gcagacagga gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt tgattatctt
    19201 ctttgtcggg atcaacttcc tgattcgttt cgtcatggct aaagtcttcc aagggaaaaa
    19261 acaagaagag atcgttgcga caatagaatc gaaaccaacg aatcaaaacc atgaaggggg
    19321 cgaactataa tgtcgactat cgaattaatt gccttgatcc ttacttctac actggtttat
    19381 tcaacaccgc taattctaac gtcactaggc ggaacttttt ctgaacgaag cgggatcgta
    19441 aacgtagggc tagagggaat catggtcatg ggtgctttta gttccatcgt tttcaacttg
    19501 acgatgagtc catcacttgg ttctatgaca ccatggatcg ctattttagt gggtggcgtc
    19561 gtaggtatcc tattttctct gattcacgca gtagcaacag tcagtttgcg tgctgaccat
    19621 attatcagcg ggacagtaat taatctgatg gcacctgctt taggtgtttt cttgatcaaa
    19681 gcatggtatg gtaaaggtca aactgataac atcacgcaga atctggggta tttttctttc
    19741 ccaggattag cagatattcc ggtcattgga ccaattttct ttaaaaatac attcttacct
    19801 gcttattgtg ctattttgat tgctgtccta gcttgggtga tcatcttcaa gacgaaattc
    19861 ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg ccgacacatt aggtatcaat
    19921 gtatatgcta tgcgttattc aggcgtactg atttctggct tgttaggcgg tatgggcggt
    19981 gctgtttttg ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt tggacaaggg
    20041 ttcatctcca tggctgctat gatctttggt aagtggaatc ctttaggggc aatgggtgcc
    20101 tcattgttct ttggttttgc tcaaagcttg agtatcattg gcgcacaatt accagttatt
    20161 tcttcgattc cgccagtatt gctgcaaatt gctccatatt tgctgacaat cattgtgctg
    20221 gttgttttct tagggaaagc ttctggtcca aaagcaaacg ggaagaacta tatcaaatca
    20281 aaataatctt ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg tctcgccctt
    20341 tttgttttaa aggcaataaa aataaaaaga aagaaggaat aatcatgttt aaacgcgtac
    20401 atctgatcgt catggattca gtaggaattg gagaagcacc agatgccgat aagttcggtg
    20461 atgttggttc tgatacatta ggacacattg cgaaagaagc aggattgaac gttccacatt
    20521 tagagcagct tggtttagga acgatccgtc ctcttgaagg tgtggaatca gtgaaagatc
    20581 atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg atgacaggcc
    20641 actgggaaat catgggattg aatatcaaaa ctccatttcg tgtgtttcct aatggattcc
    20701 ctgatgatct gatcaaacaa atcgaagaat tttctggccg taaagtcgta tgtaacaaac
    20761 cttatagcgg aacagctgtt attgacgatt acggtgaaca ccaaatgaaa acaggcgatt
    20821 taattgtata tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa gaaatcattc
    20881 ctttagaaga attatatcgg atttgccaat tcgttcgtga tataacaaaa gatgatccgt
    20941 atatgattgg ccgaatcatt gcacgtccgt acgtgggtga accaggagac ttcacacgaa
    21001 caagcaatcg ccatgattat gcgttgaatc cattcggaca cacagtcctt gattctttga
    21061 aagaagcagg aaaggacgtg attgctgttg gaaagatcaa cgatattttc aacggacaag
    21121 gaatcactga atctgtccgc acgaaaagca atatggatgg cgttgaccag ctgctgaaag
    21181 ttatgaaaaa cgatttttca ggaatcagct ttaccaattt agtagatttt gatgcattat
    21241 acggtcatcg tcgggatgta gtcggctatg cgcatgcgat tgaagaattt gacttacgaa
    21301 ttccagaatt gcttgaagcg atggaagaag acgacttgct aatgattaca gccgatcacg
    21361 gaaacgatcc gacgtttact ggtaccgatc atacgcgaga atatgtaccg ttattggttt
    21421 acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg atattattca gatatttcag
    21481 caacgattgc tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc ttcttgaaat
    21541 tattaaaata atctttttaa ataggagaaa gcaaatgacc aagttaagcg agatgttaca
    21601 agaaacgacc cagtttatcc ttgataaagg agtaaaagaa attgattttg gtttgattct
    21661 aggttcagga ttaggtgaat tagcagatga aatcgaagat gctgtaatga tttcttatga
    21721 tgagatcccg ttctttcctg tttcgacagt agttgggcat gcaggaaagc tagtttatgg
    21781 gtctctggca ggcaagaaag tgttagccat gcaagggaga ttccactact atgaaggtca
    21841 ttccatgcaa acagtgacct atccagtacg tgtgatggcc ggactagggg cccattcagt
    21901 cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag atttgatgct
    21961 gattaccgat catatcaatt ttactggtga aaatcctttg atcggtccga atgaagatgc
    22021 aatgggccct agatttccag atatgagtca agcttatacg cctgactatc aagcaacagc
    22081 aaaagcagca gcagaaaaac taggtttgga tcttaaagaa ggcgtatata tgggctattc
    22141 tggaccaact tatgaaacgc ctgcggagat ccgaatgtct cggacaatag gagccgatgc
    22201 agtagggatg tcgactgttc cagaagtcat cgtagcagct cacagcggat taaaagtgtt
    22261 aggaatctct tgtattacca acttagcagc aggtatgcaa gcgaatctga gccatgaaga
    22321 agttgtagaa acaactcaga gagtcaaaca atcttttaaa gcattggtta aagaaacatt
    22381 gacattattg taataggagg aacaataaaa tgagtgtgca tattgaagcg aaagaaggcg
    22441 agatcgccga taagatttta cttccgggag atcctttgcg agctaaatat attgcggaga
    22501 cttttttgga agatccagtt tgttacaatc aggtacgcgg tatgttaggc tttaccggca
    22561 aatataaagg agaacgtatt tctgttcaag gaacaggaat gggaatgcca tcagccacca
    22621 tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa attgattcga gtaggtacat
    22681 gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt actagcacaa ggtgcagcga
    22741 ccagttcttc gatgatcgaa aaaaatttcc aagcctttca ttttcctcca atcagtgatt
    22801 tcaatctatt actgaaagca tatgagatag ctaaagaaaa gggctatacg gttcatgtag
    22861 gaaatgttct ttcggaagat tctttctaca aagatgattt gacagagacg ttccaattag
    22921 ctgaacttgg cgttttagga gtggaaatgg aagcagcggc tctttattac ttaggcgcga
    22981 aataccatgt gcagacatta agtttgatga ccgttagcga ccatttgatc accggtgaag
    23041 aaacaacagc tgcagaacgt caatctacgt tcaatgaaat gatcgaagta ggattggaaa
    23101 cagcgatagc agaataagaa ggaaatgaaa aagctgccct gcattaagta ataagaacat
    23161 ccaagcaaaa atagctcctc atgtttttga cttggctgga cttattattg caaatgtaag
    23221 cttttgaaca ccgtttacca agaggttgtg aatctgccgt ttaactctga gtcacaagga
    23281 acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga ccgagaagtt
    23341 ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac aaatcgtgtc acagtccctt
    23401 ttttatagtg ttttttatag gtagtatttt ttagcaactg ttaagtcatc atttaattca
    23461 taaacaagtg gttgacctgt tggaatttca agatccatga tgtcttcgtc agagatacct
    23521 tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg cagctactaa aacagttttg
    23581 ttgtctagta atgctggagc aatttcatct tgccagaatg gcaaagcgcg ttctaatgtc
    23641 actttcaagt tttctccacc aggaatatcg cgtttgtcta acattgcgta acgacggtca
    23701 tttgctgcag aaccttcgtc agtttcttcc attaatggag gaagagtatc gtaagaacga
    23761 cgccagatgt gcacttgttc gtcgccgtat ttttcagctg tttctttttt gttcaaacct
    23821 tgtaatttac catagtgacg ttcgtttaag cgccatgatt tgatttgtgg tacccaaagt
    23881 tgatctgaat attctaagat caagttacat gttttgattg cacgagttaa aacagaagtg
    23941 taagcaacat caaattcgat tccagcttct ttgattttgc gtccgccttc ttttgcttct
    24001 tcgattcctt ctggtgctag atttacatca gcccaaccag taaattggtt caatgcattc
    24061 cattcactaa gaccgtgacg agaaaaaact aattttggca tgtttagctc tccttttaag
    24121 ataggtttat atttctgaaa ttttcagttc tgttttattg tacaattttt tggaagagat
    24181 ttccatcgaa aaattagata agattctatg agaaaacaag tagagaatat aaaaaaacga
    24241 acagtgccca cgcaactgtt cgtttttagg tattagatgc ataattgctc gatttctttt
    24301 tcaggtaaat tgatatattt ataacatgtt ccaactgaaa caccgcattt tgaagaaata
    24361 aattggatcg tttctttttt ttcataatag agacgtcgga tttttttgat cgtttttgca
    24421 tcgatttttg gtcttccgcc gatttttcct tttttacgag cttcatctaa tccaactaat
    24481 gtacgttctt tgatcaatgc gcactccatg tttgctaggt tttccattaa ttggaaatag
    24541 atttttccca tttcgcgcgt atcgattcct tcatcaagac taatcaagtg cagcccgcga
    24601 tctttgaata attgcgtgag ttctgttaat tgccgcgtag atttgcctaa tcgatgaagt
    24661 tggcaaatga ctagtgcatc gccttgttgc atgtcagcga tcactgactc caaatgaaca
    24721 gcagtagaat cattttgttc cacttcatat gtctcatgga aaatttcttg acaaccaaag
    24781 tcagataatt gatcaacctg agttgttaaa tcaacgtcat ttatggttgt gcgtgcataa
    24841 ccaataattt tcatggtgat tcctttctag cagtgatttt ctaactggat ataacagtaa
    24901 aaatgtagca taaagtacgt caaaaagata gttttttcgc aaattttccg ctgtcttttt
    24961 ttattggatg atcaagcagt ttttctttat ttacgacgga ttgcgtcaag aatctcaacg
    25021 cctttttctg agccgacaac tactttagaa accatgttta ggaaaagccc atgttctact
    25081 accccaacaa gttcatctaa ataggtagct aagctttctg gctgttcgat gacttctaga
    25141 tgcagatcaa tgatgtaatg accaccatct gtggttaaga cttctccgtt ttcgtctgta
    25201 cgcaagacgg gttggaatct tttttcttca aaaatatgaa gtagctgttg ggagccataa
    25261 gggatcactt caactgggag tgggaacttc ccaagtttat cgactaattt agaagaatca
    25321 acgatccaaa tcgtttcttt tgagtacgta gcgactattt tctcgaaaag caaagcagcg
    25381 ccgccgcctt tgatcccttg aaaatcagca ctgatttcat cagcaccgtc tattgtcaaa
    25441 tcgactacgg ggacttcatc aatactcttt aacggtattc ctaatttttc cgcttgttct
    25501 tttgtcgcat ttgaagtagt aactcctgtg atacgtaaac cttcttcacg catacggcga
    25561 ccaatctctt ctaccatata ataggcggtt gaaccggtcc cgagacctac gatcatcccg
    25621 tcctttacga atttcgcggc ttcaatgcca gctaattgtt ttaaattcat ctttgcgtcc
    25681 accttttttt agtttgagca tcgtgctttc ctcactattt tagcgtgatt tacggtaaaa
    25741 agataccatt cttttttatt ccaataaagc tttttcaaaa aaaattgcat ctcttgttga
    25801 catgtttttt cattcgtgat attctaacaa aggtgcttta tctacaggtt tctgtgaaga
    25861 aacgtgctga ctgtttatca aagcatattt gataaggatg cagacattgc atttattttt
    25921 tatcacaaaa aaagaaccac ctggatgtgt ggaactagac gcaaatcgtg ggaaggagga
    25981 aacaaggaat gcctacaatt aaccaattag tacgtaaacc tcgtaaatca aaggtggaaa
    26041 aatctaattc acctgctttg aacaaaggat ataacagctt taagaaaact caaactaacg
    26101 taaactcacc tcaaaaacgt ggggtagcaa ctcgtgtggg tacaatgaca cctaaaaaac
    26161 cgaactcagc tttacgtaaa tatgcacgtg ttcgtttgtc aaacttaatt gaagttactg
    26221 cttatatccc agggattggt cataacttac aagaacacag tgttgtatta ttacgcggtg
    26281 gtcgtgtaaa agacttacca ggggtacgtt accatatcgt tcgcggtgct ttagatactg
    26341 ctggtgttaa cgaccgtaaa caaagccgtt ctaaatacgg aactaaaaaa cctaaagctt
    26401 aattttaatt attaaacaaa tgaaacttgc ttctactata tagaagtaat tactatgaac
    26461 aatatttcgg aaggaggagt tacggatgcc acgtaaaggt cctgttgcaa aacgtgatgt
    26521 tttaccagat cctatttata actcaaaatt agtaactcgc ttgatcaacc gtgtaatggt
    26581 tgacggaaaa cgcgggattg cagctaacat tatctataat gcctttgact tgatcaaaga
    26641 atctacaggt aacgatccat tagaagtgtt tgaacaagca atgaaaaacg ttcaacctgt
    26701 tcttgaagtt aaagcacgtc gtgttggggg ttcaaactac caagtaccag ttgaagttcg
    26761 ccctgaacgc cgcgttactc taggtcttcg ttgggtagta aactacgctc gcttgcgcgg
    26821 tgaacacaca atggaagaac gcttagcaaa agaaatcatg gatgctgcaa acaacacagg
    26881 tgcttctgtt aaaaaacgtg aagacactca taaaatggct gatgccaacc gtgcgtttgc
    26941 tcactatcgc tggtaagatt ctctctgttt ggtcggcttt tataagtcga cctcagatag
    27001 tgtccttatt tttagcaact agatagtaaa gagaggagaa acaaggagaa tgaccagaga
    27061 attttcttta caaaacactc gtaatatcgg tatcatggcc cacatcgatg ctggtaaaac
    27121 gactgctact gaacgtatct tgtactatac tggtaaaatc cataaaattg gtgaaactca
    27181 tgaaggagct tcacaaatgg actggatgga acaagaacaa gaacgtggta ttacgatcac
    27241 ttctgctgct actacagcac aatggaaggg tcaccgaatc aacatcatcg acacaccagg
    27301 acacgtagac ttcacagttg aagttgaacg ttcattgcgt gtacttgatg gtgctgtaac
    27361 tgttcttgat gcacaatcag gtgttgaacc tcaaactgaa acagtttggc gtcaagcaac
    27421 aacttatggt gtacctcgta tcgtatttgc aaacaaaatg gacaagatcg gtgctgactt
    27481 cttatattca gtatcaactt tacatgaccg tttgcaagca aacgctcacc caatccaatt
    27541 accaattggt gcggaagacg acttcacagg aattatcgac cttgtaacaa tgaaggctga
    27601 aatgtataca aacgatcttg gaacagagat cgaagaaaca gaaattcctg aagaataccg
    27661 cgaattagct gaagaatggc gcgaaaaatt ggttgaagct gtagctgaaa ctgatgaaga
    27721 acttactttg aaatacttag aaggtgaaga aatcactgaa gctgagttaa aagaaggtat
    27781 ccgccgcgca acagttaacg tagaattcta cccagtacta tgtggttcag catttaagaa
    27841 caaaggggtt caattactat tggacgctgt tcttgattac ttgccatcac cgttagatat
    27901 cccagctatc aaagggattg accctaaaac tgatgaagaa gtagaacgtc ctgcagacga
    27961 cagcgctcct ttctcagctt tagcatttaa agttatgact gacccattcg taggtcgttt
    28021 aactttcttc cgtgtgtact ctggtgtttt acaatcaggt tcatacgtac aaaacgcaac
    28081 aaaaggcaaa cgtgaacgtg ttggtcgtat cctacagatg catgccaact ctcgttcaga
    28141 aatctctgaa gtttacgctg gagatatcgc tgctgctgtt ggtttgaaag acacaactac
    28201 tggggacaca ctatgtgatg aaaagagtct agttattctt gaatccatgg aattcccaga
    28261 accagttatc caagtcgcta ttgaacctaa atcaaaagct gaccaagata aaatgggtgt
    28321 ggctttacaa aaactatctg aagaagatcc tactttccgt gctgaaacaa acgtggaaac
    28381 aggcgaaaca atcatcgctg gtatgggtga acttcactta gatatcatcg ttgaccgtat
    28441 gcgtcgtgaa ttcaaagttg aagctaacgt aggtgcgcct caagtatctt accgtgaaac
    28501 tttccgtgct ggcactcaag ccgaaggtaa gtttgtacgt cagtctggtg gtaaaggtca
    28561 atacggtcac gtatggattg aatttacacc aaatgaagaa ggtgctggtt tcgaatttga
    28621 aaatgcgatc gtcggtgggg ttgttcctcg tgaatacatt ccagctgttg aaactggttt
    28681 gaaagatgct atggaaaatg gtgtcttagc tggctatcca ttagtagata tcaaagctaa
    28741 actttacgat ggttcatacc atgatgtcga ttcaaatgaa actgccttcc gtgttgctgc
    28801 ttctatggca ttacgtgcag cagctaagaa agctaatcca gtaatccttg aaccaatcat
    28861 ggctgtagaa gttgttatcc ctgaagatta tttaggtgat gttatgggtc acgtaactgc
    28921 tcgtcgtgga cgtgttgaag gtatggaagc tcgcgctggc ggccaacaag ttgttcgtgc
    28981 gatggttcca cttgctgaaa tgtttggtta tgctacaact cttcgttctg ctactcaagg
    29041 tcgtggtaca tttacaatga catttgacca ctacgaagat gtaccaaaat cagtacaaga
    29101 agaaatcatt aagaaaaacg gcggaaaagc tgaataattt cttaaaaccg gatagtcctg
    29161 caaagagatt atccaaagag actatatttt taatatagtt tcgtgtaaaa taattatcga
    29221 tgatatgggc atgtggtgta ccttgtgtac atcatatacc tatcagacaa ataaaaaata
    29281 tattgtattt ttaggaggac attttaaaat ggcaaaagaa aaatttgacc gttctaaacc
    29341 acacgttaac attggtacta tcggacacgt tgaccatggt aaaactacat taacagctgc
    29401 aatcacaact gtactatcta agaaaaacgg cggccaagct atggcttacg atcaaatcga
    29461 tggtgctcca gaagaacgcg aacgcggaat cacaatctca acagctcacg ttgagtatga
    29521 aacagataca cgccactacg ctcacgttga ctgcccagga cacgcggatt acgttaaaaa
    29581 catgatcact ggtgctgctc aaatggacgg agctatcttg gtagtttctg ctgctgacgg
    29641 cccaatgcct caaactcgtg aacacatcct attgtctcgt caagttggtg ttccttacat
    29701 cgttgtattc ttgaacaaag tagacatggt tgatgacgaa gaattactag aattagttga
    29761 aatggaagtt cgtgacctat taacagaata cgaattccct ggtgacgatg ttcctgtagt
    29821 tgctggatca gctttgaaag ctctagaagg cgacgcttca tacgaagaaa aaattcttga
    29881 attaatggct gcagttgacg aatacatccc aactccagaa cgtgacaacg acaaaccatt
    29941 catgatgcca gttgaagacg tgttctcaat tactggacgt ggtactgttg ctacaggtcg
    30001 tgttgaacgt ggacaagttc gcgttggtga cgaagttgaa gttgttggta ttgctgaaga
    30061 aacttcaaaa acaacagtta ctggtgttga aatgttccgt aaattgttag actacgctga
    30121 agctggagac aacattggtg ctttactacg tggtgttgca cgtgaagaca tccaacgtgg
    30181 acaagtttta gctaaaccag gtacaatcac acctcataca aaattctctg cagaagtata
    30241 cgtgttgaca aaagaagaag gtggacgtca tactccattc ttcactaact accgtccaca
    30301 attctacttc cgtacaactg acgtaacagg tgttgttgaa ttaccagaag gaactgaaat
    30361 ggttatgcct ggcgacaacg taacaatgga agttgaatta atccacccaa tcgctatcga
    30421 aaacggtact aaattctcaa tccgtgaagg cggacgtaca gttggtgccg gtgtcgttac
    30481 agaaatcaaa gcttaattca cttaagtttt aaagtaaagc aacatttatt cggacgtaat
    30541 cgctcggaac gaaggtctta ggatctttgt tccgagcttt tttatttatt gcaggatcag
    30601 gaaaaaagac aggagtatta taaatagaag aaacacgaaa taaaagagtc agtattttct
    30661 aaatgaaact ttatgaaaac aaaatgtttt ttacaagaaa aagccttgct ttgtgagttt
    30721 atatatatta taatgacaaa ggtgcgttta ctttagtgaa ttgcacagac gtaatcagaa
    30781 tgatttcgtc ggctaagaaa cgagaggttg cgacacgccc ggacgctttg ccacgaacga
    30841 gcgtgacgga aattttcgtg gagctatgtc tacttttcaa aataggcgaa ggagggaaca
    30901 agatggcaaa acaaaaaatt cgtatccgtt taaaagcgta tgaacaccgt attttagatc
    30961 aatcagcgga taaaatcgtg gaaactgcaa aaagaactgg agctagcgtg tctggtccaa
    31021 ttccattacc aactgaacgc agtctttaca caatcatccg tgcgactcat aaatacaaag
    31081 attcacgcga acaattcgaa atgcgtacac acaaacgtct tatcgacatt gtgaacccaa
    31141 caccaaaaac tgttgatgct ttgatgaagc tagacttacc atctggtgta aacatcgaaa
    31201 tcaaattata aaactaaaac aaaaataatg gaggtgtact catgaccaaa ggaatcttag
    31261 ggaaaaaagt gggaatgaca caaatcttca ctgaatctgg tgaattaatt ccagtaactg
    31321 ttgtagaagc tactccaaac gtagtactac aagtgaaaac aatggaaacg gatggctacg
    31381 aagctattca agttggttac caagatatgc gtgaagtttt atcaaacaaa cctgcgaaag
    31441 gtcatgttgc aaaagcaaac acggctccta agcgcttcat tcgtgaattc aaaaatgttg
    31501 agctaggaga atatgaagta ggaaaagaaa ttacagtaga tgttttccaa gcaggagaca
    31561 ttattgatgt aacaggtact acgaaaggta aaggattcca aggggttatc aaacgccacg
    31621 gacaaagccg cggaccaatg gctcacggtt ctcgttacca tcgtcgtcct gggtcaatgg
    31681 gtccagttgc acctaaccgt gtatttaaaa acaaacgtct tgctggccgt atgggtggca
    31741 accgcgtaac tatccaaaac ttggaagttg tacgtgtcga tgcagaaaga aatgttatct
    31801 tgatcaaagg taatgttcct ggagctaaaa aatctttaat tactatcaaa tcagctgtga
    31861 aagctaaata attgtaggaa gaaaggagga actaaggaat gccgaatgta gcattattca
    31921 aacaagatgg aagccaaaac ggtgaaatca cacttaacga agaaatcttc ggaattgaac
    31981 ctaatgaaag tgttgtttac gatgcaatcg taatgcaacg tgcgtcatta agacaaggaa
    32041 cacacgcggt taaaaaccgt agcgctgttc gcggcggcgg acgtaaacca tggcgtcaaa
    32101 aaggaacagg tcgtgcacgt caaggatcta tccgctcacc acaatggcgt ggaggtggcg
    32161 tagtctttgg accaacacca cgttcttaca gctacaaact tcctaaaaaa gttcgtcgtt
    32221 tagcaatgaa atctgtattg tcagaaaaag ttgctgaaaa caacttggta gcaatcgaag
    32281 gcttgaactt cgacgcacca aaaacaaaag aattcaaaca agttcttgct aacttatcta
    32341 tcgactctaa agtattggtt gttctagaaa aaggtaacga ctttgctgct ttatcagcac
    32401 gtaacttacc aaacgtttct gtagtaactt ctgataatgt gagtgtgcta gacgttgtat
    32461 caaatacaaa agtgttagca acacaaactg ctcttactca aattgaggag gtgcttgcat
    32521 aatgaactta ctagacgtaa tcaaacgccc agtgatcact gaaaaatcaa tgcttgccat
    32581 ggatgacaag aaatacactt ttgaagtgga cactcgcgca aacaaaactt tagtaaaaca
    32641 agcggtagaa gctgcttttg acgttaaagt caaaaacgtg aacatcgtga acgtacgtgc
    32701 gaaattcaaa cgtatgggca aatatgcagg atatacaaaa aaacgtcgca aagcgatcgt
    32761 tacattgact gaagattcaa aagaaatcca attattcgaa gctgctgaat aagcactaat
    32821 agataactca ttaaattagg agggaaaaac gtggcgatta aaaagtacaa acctacctca
    32881 aatggtcgtc gtaacatgac aggttctgat tttgctgaaa tcacaacatc aacacctgaa
    32941 aaatcattgt tacaaccatt gaaaaaccat gctggacgta acaacaacgg ccgcatcact
    33001 gtacgtcatc aaggtggcgg acacaaacgc caatatcgcg tgatcgactt caaacgtaac
    33061 aaagataacg ttgttgcaac tgttcaaacg atcgaatatg atccaaaccg ttcagctaac
    33121 atcgcgttag tacactatga agatggagtg aaagcttaca tcttagcacc taaaggacta
    33181 gaagtgggta tgaaacttgt ttcaggtcca gaagcagata tcaaagtcgg aaatgctctt
    33241 ccattagaaa acattccagt tggtactgtt atccacaaca ttgaaatgaa acctggtaaa
    33301 ggtggacaat tgatccgttc tgctggtaca agtgctcaag tacttggtaa agaaggcaaa
    33361 tacgtattga tccgcttgaa ctctggtgaa gttcgtatga tcttagctgc atgccgtgct
    33421 acaatcggtt ctgtaggtaa cgaacaacac gaattaatca acatcggtaa agctggacgc
    33481 tctcgttgga tgcgtaaacg cccaactgta cgtggtagcg taatgaaccc taatgatcac
    33541 ccacacggtg gtggtgaagg taaagctcct atcggacgta aagcaccagt ttctccttgg
    33601 ggtcaaccag ctcttggtta caaaactcgt aacaaaaaag ctaaatcaga caaacttatt
    33661 gttcgtcgtc gcaaaaataa ataattactt tgaccatgtg tcgcacattt tgagaggagg
    33721 ttcaccatgg gtcgtagttt aaagaaagga ccttttgtcg atgagcattt aatgaaaaaa
    33781 gttgaagctc aagcaggggt tgaaaagaaa aaagttatca aaacttggtc acgccgttca
    33841 acagttttcc caagttttgt tggatatacc atcgcagttt atgatggacg taaacatgta
    33901 ccagtttaca tccaagaaga catggtagga cacaaattag gtgaatttgc acctacaaga
    33961 acttatcgtg gacatgctgc tgacgataag aaaacgaaac gctaatttga ggggaggata
    34021 aatccaaatg gcagaacaaa tcacatcagc taaagcaact gcaaaaacag ttcgcacttc
    34081 acctcgaaaa gcccgtttag taattgacct tatcagaggt aaaagtgtcg cagatgcaat
    34141 ttcaatcttg aaattcacac caaacaaatc tgctggaatc attgaaaaag tattgatgtc
    34201 agcagttgcg aatgcagaaa ataactttga cttagatgtt gaaaacttgg tagtaagcga
    34261 agcttttgtt aacgaaggac ctacaatgaa acgtttccgt cctcgtgcaa aaggatcagc
    34321 ttctccaatc aacaaacgta caagtcatat cacggtagtc gtatcagaaa aataaggagg
    34381 gataatcagt gggtcaaaaa gtacatccaa ttggaatgcg tgtaggcatc atccgcgact
    34441 gggatgctaa atggtatgct gaaaaagagt atgctgagtt cttacacgaa gatttaagaa
    34501 tccgtaaatt tatttcaact aaacttgctg atgctgctgt atcaacagtt gaaatcgaac
    34561 gcgcaacagg tcgcgtaaac atttcaattc acacagctaa accaggtatg gttattggta
    34621 aaggcggttc tgaagtcgaa agcctaagaa aagaattaaa caaattaact ggtaaaagaa
    34681 tccatatcaa tatcgtggaa atcaaaaaac cagatctaga tgcaaaatta gtaggtgaag
    34741 gaatcgcacg tcaattagaa aaccgtgttg ctttccgtcg cgctcaaaaa caagccatcc
    34801 aacgctcaat gcgtgctggc gctcaaggga tcaaaactca agtatctggt cgtctaaacg
    34861 gagcagatat cgctcgttca gaaggatact cagaaggaac tgttcctctt catactttac
    34921 gtgcggacat tgattacgca tgggaagaag cagatacaac atacggaaaa ctaggagtta
    34981 aagtgtggat ctatcgtgga gaaattcttc caactaaaaa aaacactgag aaaggaggga
    35041 aataatcatg ttagtaccta aacgtgtaaa acaccgtcgt gaattccgcg gaaaaatgcg
    35101 cggggaagct aaaggcggaa aagaagtagc attcggtgaa tacggtttgc aagctgttga
    35161 ttcacattgg atcacaaacc gccaaatcga agctgctcgt atcgcaatga ctcgttacat
    35221 gaaacgtggt gggaaagtat ggattaaaat tttccctcac aaatcttata ctgccaaagc
    35281 aattggggta cgtatgggtt ctggtaaagg ggcacctgaa ggatgggttg caccagtaaa
    35341 acgtggtaaa atcatgtttg aaatcgcagg cgttcctgaa gaagtagctc gtgaagcgtt
    35401 acgtctagct tctcacaaat taccaatgaa aactaagatc gtaaaacgtg aggaaatggg
    35461 tggtgaatcg aatgaaggtt aaagaaatca gagaattaac cactgccgaa atgcttgatc
    35521 aagaaaaaca attaaaagaa gaattgttta atcttagatt ccaattagca acaggtcaat
    35581 tagaaaacac tgcacgtatt aaagaagtac gtaaatcgat tgcacgcatc aaaactgtgt
    35641 tgcgtgaaca agctaagtaa ttatggaagg aggccattaa gcgtatgact gaaggaagaa
    35701 atcaacgcaa agtttatcaa ggtcgcgtgg tttcagacaa aatggataaa acaattaccg
    35761 ttgctgtcga aacaaagaaa aaccacccta tctatggcaa acgcatgaac tattctaaga
    35821 aatacaaagt gcatgatgaa aacaacacag caaaagttgg cgacatcgtg agaattatgg
    35881 aaactcgtcc attatcagct acaaaacgtt tccgtttgtt ggaagtagtc gaagaagcag
    35941 ttattatcta ataacacgag attttcctat atagattgaa tgcaaaatct gaaaggagga
    36001 tacacaacgt gatccaagca gaaagtcgtt taaaagtcgc tgataactca ggcgcacgtg
    36061 aaattttgac gatcaaagtc cttggtggat ctggtcgtaa aactgctaac atcggtgatg
    36121 tgatcgttgc tacggttaaa caagcaacgc caggtggggt tgtcaaaaaa ggtgacgtcg
    36181 ttaaagccgt tatcgttcga acaaaatcag gagctcgtcg tacagacggt tcatatatta
    36241 aatttgatga aaatgctgca gtgattattc gtgatgataa aagccctcgt ggaacacgta
    36301 tctttgggcc agttgcacgt gaactacgtg aaaacaactt catgaagatc gtttcactag
    36361 caccagaagt tttataatct tgagacactt atcaaaggag gtgcgaaaca gttatgtttg
    36421 ttaaaaaagg cgataaagtt aaagttatca ctggaaaaga caaaaacaaa gaaggcgttg
    36481 tactagcagc gttcccgaaa aaagataaag ttattgtcga aggtgtaaat gtcgttaaaa
    36541 aacaccaaaa accttctcaa gctgctcctc aaggtggaat tgttgagatg gaagcgccaa
    36601 ttcatgtttc taacgtgatg gtggttgact ctactggtgt agctggccga gttggctaca
    36661 aagaagtaga cggcaaaaaa gtccgtgttt ctaaaaaaac cggtgaagtt ttagataaat
    36721 aaaagtttaa ggaaggaggg tcttactgaa tgaaccgcct aaaagaaaaa tatcttaaag
    36781 aagtaactcc atcattgatg gaaaaattta actatagctc tgttatgcaa acacctaaag
    36841 ttgaaaaaat cgtcatcaac atgggtgttg gtgatgcggt atcaaacgct aaaaacttag
    36901 ataaagcagt tgaagaatta actttgatca ctggtcaaaa accaatgatt acaaaagcta
    36961 aaaaatcaat cgctggattc cgtttacgtg aaggaatgcc aattggtgca aaagttacat
    37021 tgcgcggaga aagaatgtac gaattcttag acaaattggt ttctgtttca ctaccacgtg
    37081 tacgtgactt ccatggtgta agtaaaaaag cctttgacgg tcgtggaaac tatactttag
    37141 gtattaaaga acaattgatc ttcccagaag tagattatga tttagtagat aaagtacgtg
    37201 gtatggacat cgttattgta acaacagcaa actctgatga agaatctcgt gagttgttga
    37261 cacaattagg catgccattc caaaaataat taaaggaggc gaactacgtg gctaaaaaat
    37321 caatgattgc taaaaacaaa cgtcctgcaa aattttcaac acaagcttat actcgttgcg
    37381 aacgttgcgg acgtccacat tcagtttatc gtaagttcca tctttgccgt atttgcttcc
    37441 gcgaacttgc ctataaaggt caaattcccg gcgtgaagaa agctagctgg taaaaagtaa
    37501 attcgcataa gaaggaggta aaagatcaat ggtcatgaca gatccaattg cagattttct
    37561 aactcgtatc cgtaacgcaa acatggttaa acatgagtct ttagaagtac ctgcatcaaa
    37621 aattaaacgt gacatcgctg aaatcttgaa acgtgaaggt ttcgttcgtg atgttgaata
    37681 catcgaagat gacaaacaag gcgtgatccg tgttttccta aaatttggta aaaacggcga
    37741 acgtgttatc acaaacttaa aacgtatctc taaaccaggt ttgcgtgctt atgtcaaagc
    37801 tgacgaagta ccaaaagtat tgaacggttt aggtatcgca atcatttcga cttctgaagg
    37861 tgttgtcact gataaagaag ctcgtgaaaa aaatatcggc ggcgaagtta tcgcttacgt
    37921 atggtaatca agtagaatct acaaggaggt gtctttaagt gagccgtatc ggaaataaag
    37981 tcgttgttat ccctgaagga gtaacagtta ctcaagacgg aaacaacgtt acagttaaag
    38041 gaccaaaagg tgaattgact cgtacttttt cagcagatat caaaatgaat atcgaaggaa
    38101 acgaagtaac tttcactcgt ccaaacgaca gtaaagaaat gaaaacaatt catggaacaa
    38161 ctcgtgcgaa cttcaacaac atggttgtag gtgtcagcga aggtttccaa aaagcgttag
    38221 aactaatcgg ggttgggtac cgtgcacaat tacaaggatc aaaacttgta ttgaacgttg
    38281 gttactcaca tccagtagaa atcacaccac cagctggagt aactgttgaa gtaccagcta
    38341 acacacaagt aattgttaaa ggtgcgaaca aagaagaagt tggcgaatta gctgcaaaca
    38401 ttcgtggaac tcgtcctcca gaaccttata aaggtaaagg tattcgttat gttggcgaat
    38461 atgtacgccg taaagaaggt aaaactggta aataatcgtt tagaaaaatt gcatagaagc
    38521 cagcagcagg aaaaaggcat ttggctgaag tttaccttca aaaaaataca tttttacttg
    38581 tgccgcagct tctatttttc gaaacgtatt tactgcttga aatagtagta caataaaaat
    38641 aaagaggtga caattgtgat tacaaaacca gataagaaca aaacacgtca aaagagacat
    38701 cgtcgtgtac gtaacaatat ctctggtact gctgagcgcc cacgcttgaa cgtttttcgt
    38761 tctaacaaaa acatctacgc tcaaattatt gatgacgtag cgggtgtaac gctagcaagt
    38821 gcctctacct tggataaaga aatttcaggt ggaacaaaaa cagaagcagc acaagctgtc
    38881 ggtaaactag ttgctgaacg tgcagctgaa aaaggcatta aagtagtagt ctttgaccgt
    38941 ggtggatacc tttaccatgg ccgtgtgcaa gctttagctg aagctgctcg cgaaaatgga
    39001 ctagaatttt agaagaagga ggaataccat tcatggttta tatcgatcca aaacatttgg
    39061 aattagaaga ccgcgttgtt gcaattaacc gtgtaacaaa agttgttaaa ggtggacgtc
    39121 gtctacgttt tgctgcttta gttgttgtcg gtgacaaaaa cggacacgta gggtttggta
    39181 caggtaaagc tcaagaagtt cctgaagcaa tccgtaaagc aattgaagat gcgaagaaaa
    39241 acttagttga agtacctatg gttggttcaa caatcccaca cgaagtaatc ggtgctttta
    39301 gtggtggtag aatcctaatg aaacctgctg tagaaggttc tggggttgct gctggtggac
    39361 cagttcgtgc cgtattagaa ttagctgggg tagcagatat cacttctaaa tctttaggct
    39421 caaatacacc aatcaacgtt gttcgcgcaa cagttgaagg tttgaaacaa ttgaaacgtg
    39481 cagaagaagt tgcagaactt cgcggcaaat cagttgaaga attaatcgga taaggaggac
    39541 aacaataatg gctgaattaa aagttacttt aaaacgcagt attatcggac gtcctcaaaa
    39601 ccaacgcgat acagttaaag cgttaggtct tacaaagatt aacagttcag tggttaagcc
    39661 ttctaatgaa gcaattaaag gcatgatcaa tactgtttct cacctagtgg acgttgaaga
    39721 aatttaattt agaaaaatca taactagaac attaaggagg tgccaaacct atgaaacttc
    39781 atgaattaaa acctgctgaa ggttcacgcc aagtacgtaa ccgtgttggt cgtggtactt
    39841 catctggtaa cggaaaaact gccggacgcg gacaaaaagg acaaaaagct cgttcaggcg
    39901 gcggtgtgcg tttaggattt gaagggggac aaacaccatt gttccgtcgt ttaccaaaac
    39961 gaggattcac aaacgtgaac cgtaaagact atgcagtcat taatcttgat gtattgaacc
    40021 gctttgaaga cggagctgaa gtaactcctg ttgctttagt tgaagctgga atcgtgaaaa
    40081 acgaaaaagc tggaatcaaa gtattagcta atggggaact aaacaagaaa ttaactgtga
    40141 aagcagcaaa attctctaaa gcagctcaag aagcaattga agcggctggt ggatcaatcg
    40201 aggtgatcta atgtttagac tattaaaaga tgcttttaaa gtaaaagaca ttagatcgaa
    40261 aatcctattt actgtatttg tgttgtttgt atttcgccta ggtgcacata ttactgtacc
    40321 aggcgtaaat gctgcttcat tgcaaaacct ttcttctttg ccatttttca acatgttgaa
    40381 cttggtaagt ggtagtgcga tgcaaaactt ttccattttc tcaatggggg tttcaccata
    40441 cattactgct tctatcgtca ttcagcttct tcaaatggac atcgtgcctc gatttgttga
    40501 atggtcgaaa caaggggaag ttggtcgtaa aaaactgaat caagcgactc gatatctgac
    40561 attagtattg gcatttatcc aatctgtcgg tatcactgct ggattcaatc aatacgctca
    40621 attaggtttc gtgaataatc ctaacgtagc aacttacgta acgatcggtt tgattctaac
    40681 agccggtacg atgtttgtta cttggctagg agaacagatc acagaaaaag gtatcggaaa
    40741 tggcgtatca atgatcattt ttgcaggtat catttctaga ttgcctgaaa gtatcagaga
    40801 aatttatgaa gattacttta taaatattga tccttcagac atatggaaat ctgctatttt
    40861 tgtggccatt ttactagtag cggtcttgtt gatcgtgaca ttcgtaacat ttttccaaca
    40921 agctgaacgc aagatcccga tccaatatac gaaacgcgta gctggcgcgc ctacaagcag
    40981 ctatctgccg ttgaaagtaa atgcagctgg agttattcca gttatctttg ctagttcgtt
    41041 tattgctacg ccaaatgctg ttttacaagc attgagcggt agattcggtt ctgaaaactg
    41101 gtacaaagtg atgatggaga tctttaacta taatacagca cctggtgcta cgatttatac
    41161 gattctgatc gttgccttca ctttcttcta cgcctttgtt caagtaaacc ctgagaaatt
    41221 agcggaaaac ttgcaaaagc aaggaagtta tataccaagc gtacgccctg gtaaggggac
    41281 tgaagagtat gtctcaagat tactgatgcg tttaagtaca gttggtgcag tcttccttgg
    41341 tttagtggca ttgcttccaa tcattgctca aatggtttgg gatcttccac aatcaatcgg
    41401 attaggcggt acaagcttac tgatcgttat cggtgtagct ttagaaactg cgaaacagtt
    41461 agaaggctta atgctgaaac gtaaatatgt tggattcatt gagtaagaaa gcgtgttgag
    41521 gtttcccctc aacacacgtt ctcacacatt tattaggagg gaaaacaatg aacctcattt
    41581 taatggggct gccaggcgca ggtaaaggaa cacaagctga aaaaatcatt gatgcttatg
    41641 gtattccgca catctcaaca ggagatatgt tccgcgcagc gatgcaaaac gaaacagctc
    41701 ttggcctgga agcaaagtct tatatggata aaggtgcatt agttcctgat gaagtaacaa
    41761 atggaatcgt gaaagagcga ctagctgaac cagatacaga gaaaggcttc ttattagatg
    41821 gctttccacg tacgctagat caagcaaaag ctctggacgc aatgctaaaa gatttgaata
    41881 aaaaaattga tgctgtcatc gatatccatg ttggtgaaga aatcttggtc gaacgtctag
    41941 ccggccgatt tatctgcagc aattgtggag caacttacca taaaattttc aaccctacaa
    42001 aagttgaaga cacatgtgat cgttgcggtg ggcatgaatt ctatcaaaga gaagatgata
    42061 aacctgagac ggttaaaaat cgtctggcta tcaacatcaa aaatagtgaa ccaatcttgg
    42121 cttattataa agaacaaggt ttgctaaaca ctattgatgg tgctcgtgaa atcgatgctg
    42181 tatttgcaga tgtacaaaaa atcattgaaa aatagcacga ttgcccgtag ccgacttgca
    42241 ttatgcagtt actcttgtca agttcaataa attatgatag aatatgtcag tccgacttct
    42301 agagaatcct ctagatgatc cattttgaga attttgaggt gggaactgca gtttccgcca
    42361 ttttatcgtg tgaaaatgcg aaacaagtac aaggaggtac tgtgcgtggc aaaagaagat
    42421 atgattgaag tcgaaggtac agtcgtcgaa actttgccga atgcaatgtt taaagttgaa
    42481 ctagaaaacg gacaccaagt tcttgctact gtttcaggta aaattcgtat gcattacatc
    42541 cgtatcttac ctggtgacaa agtaacggtt gaattgtctc catacgatct taaccgtggc
    42601 cgtattactt atcgctttaa ataattgtac tccgtaaaac tacaggaggt attatcatga
    42661 aagtaagacc atcagtaaaa ccaatgtgcg aacattgtaa agtaattcgt cgtaaaggac
    42721 gcgttatggt gatttgccca gcaaatccaa aacataaaca acgtcaagga taattggagg
    42781 tgctataaat atggctcgta ttgcaggagt agatattcct cgtgataaac gtgtagtagt
    42841 ttcacttact tatatctatg gtattggtaa cactactgct aaaaagatct tagctgatgc
    42901 tggtgtatct gaagatgttc gcgttcgtga attaacgaat gaacaaacag atgctatccg
    42961 tgcagaagta gataaattaa aagttgaagg ggatcttcgt cgtgaagtga acttgaacat
    43021 caaacgtttg atggaaatcg gttcttaccg tggaatccgt catcgccgtg gattgcctgt
    43081 tcgtggacaa aacacgaaaa acaatgcacg tactcgtaaa ggcccagctc gcgctatcgc
    43141 tggcaagaaa aaataattct taagtgaagg aggttaaaac ttcatggcag caaaaaaagt
    43201 gaatcgtaaa cgccgtgtga agaagaatat agaagcaggt attgcacata tccattctac
    43261 attcaataac actatcatca tgatcactga tactcacgga aatgcattag cgtggtcatc
    43321 agctggttca ttaggtttca aaggaagcaa aaaatcaact ccttttgccg ctcaaatggc
    43381 agcagaaact gctacaaaag cagcaatgga acacggatta aagactgttg aagtaacagt
    43441 taaaggacct ggttctggac gtgaagcagc aattcgttca ttacaagcta caggtttaga
    43501 agtgactgca attcgtgacg tgactccagt tcctcataat ggtgcccgcc ctccaaaacg
    43561 ccgtcgtgtt taatgagtgc cgctcatttt gagttttatc agtaacgtca ttgaacaaga
    43621 cacttttcgt tttgaaaggg gtaaagataa gaatgattga gttcgaaaaa ccaagaatcg
    43681 caaaaattga tgaagaaaaa gattatggca agttcatcgt tgaacctctt gaaagagggt
    43741 atgggactac tttagggaat tccctacgtc gtattttatt atcttctttg ccaggtgctg
    43801 ccatcactaa tattcaaatc gatggtgtac tgcatgaatt ctctaccatc aaaggtgtta
    43861 gagaagacgt cactcaaatc atcttgaaca ttaaaggctt agcgctgaaa atgtatggcc
    43921 aagaagaaaa aacccttgaa atcgatatta ccggacctgc tacagtaact gctggcgata
    43981 ttatcgttga tagtgatgta gaaattctta acaaagatat gtacatctgt agtgtctctg
    44041 aaggagctac gttccatgct cgcttaacag tgaaacctgg acgtggttat gttcaagcag
    44101 atgaaaataa aaaagaagat atgccaattg gcgttcttcc agtcgattca atctacacac
    44161 ctgttcgtcg tgtgaactat caagttgaga acacacgtgt tggacaccgt gaagattttg
    44221 ataaattgac gatggagatt tggacagatg gttcaattga accgttggaa gcaatgagtt
    44281 tgtctgcgaa aatcatgact gaacatttgg atatttttgt gaacttaact gatgaagcaa
    44341 aaaatgctga aatcatggtc gaaaaagaag aaacacaaaa agaaaaaatg ttggaaatga
    44401 cgatcgaaga gttagacttg tctgttcgct cttacaattg tttgaaacgt gcaggaatca
    44461 atactgtaca agaacttaca aataaatctg agcctgaaat gatcaaagtc cgtaacttag
    44521 gacgtaaatc attagaagaa gttaaagcaa aattgcacga cttaggttta ggtttacgta
    44581 aagaagatta aaacctttta cgaaggaggg aaaccaacgt gagttatcgt aaactaggac
    44641 gcacatctag ccaacgtaaa gcgatgttgc gtgatttaac aactgattta ttaatcaacg
    44701 aacgtattgt gactactgaa gctcgtgcga aagaagttcg ctcaactgca gaaaaaatga
    44761 tcactttagg taaacgcgga gatttgcatg cacgtcgtca agctgcggct ttcgtacgta
    44821 atgaagttgc cagcgtgcgt gaagaaaatg aggacatcgt cattgaatca gctttacaaa
    44881 aactgttcaa cgatattgca cctcgttacg cagaacgtca aggcggatac acacgcattt
    44941 tgaagacaga accaagacgc ggtgatggtg caccaatggt tgtcttagaa cttgtctaaa
    45001 atcacactaa taacatcact ttatagatga ttcttttaga gtgttatgat gttggaagat
    45061 atttttccga gtctagctca gcgctgctcc tcaagcgaat atcgcttggg gagtaagcac
    45121 tcatcataaa gtgttttttt ataccttcaa ttacctgtca tgacagatat gtttgaaggc
    45181 ataaaaaaac attttaagcg agaaataatt gaaaattttg tctttttttg atagtgttag
    45241 taggaacttt aggatgaaga aggagtaatc atggagccaa ttattgaatt agaaaagata
    45301 aattataaat accagccaga tgatcttcgc cccgcattga aagatgtctc ctttacaatt
    45361 gataaaggtg agtggatcgc tattattgga cacaatggtt caggaaaatc gacacttgcc
    45421 aaaacgatca atggattgct tctgccagaa tcagggatcg tgaaagtggg aaatcaaata
    45481 ttggatgaag aaaatatttg gacgatccga cagatggttg gaatggtttt tcaaaatcca
    45541 gacaatcagt ttgttggttc gacagtagaa gatgacgttg cttttggtct tgaaaatcaa
    45601 gggattccca gagaagaaat gctagtgcgt gtgaaagatg cacttgagaa agttagaatg
    45661 gctgagtttg cttcacgcga gcctgctcgt ttatcaggag ggcaaaaaca acgtgttgcc
    45721 attgccggcg tggttgcctt acgacctgat atcattattt tagatgaagc aaccagtatg
    45781 ctagatccag aaggtcgtga agaagtgatc tctacgatca agaagatcaa agaagaaagt
    45841 caattgaccg ttatatctat cactcatgat attgatgaag cagctaatgc caatcggata
    45901 ctagtgatga gacaaggcga acttgtccgt gaaggtacac caaaagaaat tttttctgct
    45961 ggtcctgaac tgatcgactt aggtctagat ctaccatttc ctgaaaaact gaagagcgca
    46021 ctgaaagaac gtggggttga tgtgcccagt gaatatatga cagaagaaag gatggtggat
    46081 tggttatgga catccgtttt gaacaagtag acttcactta tcagccgaat acaccatttg
    46141 aacaaagagc cttatttgac atcaatatga cgatcaaaga aaacagttat actgcattag
    46201 tagggcatac cggaagcggg aaatcaacct tacttcagca tttgaatgcg cttgttaagc
    46261 caacaagcgg tacagtccat attggagaac gagatattca gccggatacg gataataaga
    46321 atttaaagcc catccgaaaa aaagttggca tcgtctttca gtttccagaa gcacagctat
    46381 tcgaagaaac ggtagcaaaa gacattgctt tcggtcctaa aaactttggt gtcagcgaag
    46441 aagaagcact agtcctagca aaagaaacat tagaacaagt tgggctggat gaaagctatt
    46501 tggaacgttc gccatttgaa ctttcaggag ggcaaatgcg tagagtagca atcgctggtg
    46561 tgcttgccat gaggccggaa gtacttgtat tggatgaacc aacggcagga cttgatcccc
    46621 agggtagaaa agaaatgatg gagatgtttt ggcgtcttca taaggaacag cagatcacta
    46681 ttgtattagt tacgcattta atggatgatg tagccaattt tgcagattat gtctatgtgt
    46741 tggaaaaggg acgaatcgtt aatagcgggg agccgcaaga ggtctttcaa aacattgaat
    46801 ggctaaaaga aaaacaacta ggcgtaccta ctgccaccga gtttgcagaa gaattaatgg
    46861 ctaaagggat gaactttgct accttgcctt tgacagctga agaattagca gacgctatcg
    46921 tccattatgc tggaggaaat gcccatgatg aataaattga tatttggtcg ttatatgcct
    46981 ggagattcct tgatccatcg attggatccc cgagctaaat tattagccag tttttatttt
    47041 atcggaatca tttttttagc aaataattgg gaaacgtacc ttttgttggc atttttcaca
    47101 ttactttctg tcttcttgtc aaaagtagat ttgaaatttt ttatccgagg cattcgccca
    47161 ttgatctggc taatcctctt cactgtagct ttgcaggtct tgtttacaag tggcggagaa
    47221 acttattgga gttggggtgt tttccatatc actgaatttg gtgtgatgaa cggcctcttt
    47281 attttctgtc gattcgtgtt gatcattttt atgtcgactc tactcacgct tactacaccg
    47341 cctttggaat tatcggatgc gattgagtac ttgcttcgtc cattgaaagc cgtacggttc
    47401 ccagtccacg aaatttcatt gatgctgtcg attgctcttc gttttgtgcc tacattgatg
    47461 gacgaaacag aaaagatcat gaacgctcag cgcgctcgcg gtgttgattt tggtgaagga
    47521 aacctagttc agaaaatgaa agcagtcatt cctttgctga ttcctttatt tgtcagcagc
    47581 ttcaaccgtg cggaagatct tgcgactgct atggaagcaa gaggctacaa aggtggggaa
    47641 ggaagaacga aatatcgtgt attgcattgg cataatagag acacgatggt tatcattgct
    47701 tttgtattat taactgtgct actgatcttt ttgagaggat aaacgttcag tgggccgcta
    47761 tctattcttt tcttgctgat ctgtttgcaa gaaaggatgc gagcagtgta tgacaagaaa
    47821 tattagggtc tgtggcagac taaggtcaca ggcctctttc ttttatctga aaagaaagaa
    47881 cgaaaaaatt ttataaaaaa atcattattc cagataacct tttaggaaca aaggagagaa
    47941 aaacatggta cgttacaaag caatcattgc atacgacgga acaaatttta atggattcca
    48001 aaaacagcca aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc
    48061 aaatggaaaa gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat
    48121 tgggcaagta atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc
    48181 gctagatacc cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga
    48241 cttccacgca agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa
    48301 accacgcagt ccttttcgac gccattatgc aagttatttt ccttaccctc ttgatttgga
    48361 aaaaattcag cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc
    48421 atcaggaagc tcgatagagg ataaagtacg tacgatctac gaggctaaga tggaggtaaa
    48481 tgaaacaaaa gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat
    48541 acggatctta gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat
    48601 cccagatatt attgcgaaaa aagatcgtaa tgcagcagga cctacagcac atcctgaagg
    48661 actttatttg tatgaagtga aatatgaata agaggttgtg acagaagtgg tcagcttcaa
    48721 gaaataagaa ggaatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt
    48781 tcgaaggagc tacttctgga acaccgtgaa tcagaggttg tgaaaaagct gccctgcatc
    48841 aagtaataag aacaaccaaa caaaaatagc tcgtcatatt tttcgtttgg ttgggcttat
    48901 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat cagcgttttg
    48961 acctgagtat tagagaaaca aacggaaaaa tagttcctca tattttgccg tttgttgagc
    49021 taataccgaa ggtcagctga ttgaacaccg tttaagaaag aggtcgtgag cctgtcattt
    49081 aactctgagt cacaagtaac aatttcaaaa aacagcttct catctttgag gaatatcgtg
    49141 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    49201 aaaaaacgat ggaacgttgt tttgattgcc cttaatgagg acagtcggaa caggtttgtc
    49261 attttta
//
LOCUS       DAXWLG010000004        40763 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXWLG010000004 DAXWLG010000000
VERSION     DAXWLG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.004"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(32..631)
                     /locus_tag="ACGE4T_000159"
     CDS             complement(32..631)
                     /locus_tag="ACGE4T_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8385479.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            885..1766
                     /locus_tag="ACGE4T_000160"
     CDS             885..1766
                     /locus_tag="ACGE4T_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8385480.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1779..3389
                     /locus_tag="ACGE4T_000161"
     CDS             1779..3389
                     /locus_tag="ACGE4T_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8385481.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(3466..4125)
                     /locus_tag="ACGE4T_000162"
     CDS             complement(3466..4125)
                     /locus_tag="ACGE4T_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8385482.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(4408..5073)
                     /locus_tag="ACGE4T_000163"
     CDS             complement(4408..5073)
                     /locus_tag="ACGE4T_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFF8385483.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(5220..5825)
                     /gene="dhaL"
                     /locus_tag="ACGE4T_000164"
     CDS             complement(5220..5825)
                     /gene="dhaL"
                     /locus_tag="ACGE4T_000164"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFF8385484.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5853..6863)
                     /gene="dhaK"
                     /locus_tag="ACGE4T_000165"
     CDS             complement(5853..6863)
                     /gene="dhaK"
                     /locus_tag="ACGE4T_000165"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFF8385485.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6838..7233)
                     /gene="dhaM"
                     /locus_tag="ACGE4T_000166"
     CDS             complement(6838..7233)
                     /gene="dhaM"
                     /locus_tag="ACGE4T_000166"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFF8385486.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            7387..7707
                     /locus_tag="ACGE4T_000167"
     CDS             7387..7707
                     /locus_tag="ACGE4T_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFF8385487.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7819..8688)
                     /locus_tag="ACGE4T_000168"
     CDS             complement(7819..8688)
                     /locus_tag="ACGE4T_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8385488.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8784..10088)
                     /locus_tag="ACGE4T_000169"
     CDS             complement(8784..10088)
                     /locus_tag="ACGE4T_000169"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8385489.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(10085..10594)
                     /locus_tag="ACGE4T_000170"
     CDS             complement(10085..10594)
                     /locus_tag="ACGE4T_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385490.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10769..12904)
                     /locus_tag="ACGE4T_000171"
     CDS             complement(10769..12904)
                     /locus_tag="ACGE4T_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFF8385491.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12987..14642)
                     /locus_tag="ACGE4T_000172"
     CDS             complement(12987..14642)
                     /locus_tag="ACGE4T_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8385492.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(14644..14958)
                     /locus_tag="ACGE4T_000173"
     CDS             complement(14644..14958)
                     /locus_tag="ACGE4T_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8385493.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            15128..15388
                     /locus_tag="ACGE4T_000174"
     CDS             15128..15388
                     /locus_tag="ACGE4T_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFF8385494.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            15402..15716
                     /locus_tag="ACGE4T_000175"
     CDS             15402..15716
                     /locus_tag="ACGE4T_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFF8385495.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(16446..17000)
                     /locus_tag="ACGE4T_000176"
     CDS             complement(16446..17000)
                     /locus_tag="ACGE4T_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFF8385496.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(17126..19055)
                     /locus_tag="ACGE4T_000177"
                     /pseudo
     CDS             complement(17126..19055)
                     /locus_tag="ACGE4T_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(19361..20077)
                     /locus_tag="ACGE4T_000178"
     CDS             complement(19361..20077)
                     /locus_tag="ACGE4T_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8385497.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(20078..21901)
                     /gene="glpO"
                     /locus_tag="ACGE4T_000179"
     CDS             complement(20078..21901)
                     /gene="glpO"
                     /locus_tag="ACGE4T_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFF8385498.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21915..23411)
                     /gene="glpK"
                     /locus_tag="ACGE4T_000180"
     CDS             complement(21915..23411)
                     /gene="glpK"
                     /locus_tag="ACGE4T_000180"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFF8385499.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(23547..25007)
                     /locus_tag="ACGE4T_000181"
     CDS             complement(23547..25007)
                     /locus_tag="ACGE4T_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385500.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24997..26292)
                     /locus_tag="ACGE4T_000182"
     CDS             complement(24997..26292)
                     /locus_tag="ACGE4T_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8385501.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(26624..27301)
                     /locus_tag="ACGE4T_000183"
     CDS             complement(26624..27301)
                     /locus_tag="ACGE4T_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFF8385502.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(27331..28422)
                     /locus_tag="ACGE4T_000184"
     CDS             complement(27331..28422)
                     /locus_tag="ACGE4T_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFF8385503.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(28523..28957)
                     /locus_tag="ACGE4T_000185"
     CDS             complement(28523..28957)
                     /locus_tag="ACGE4T_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8385504.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(29541..30998)
                     /locus_tag="ACGE4T_000186"
     CDS             complement(29541..30998)
                     /locus_tag="ACGE4T_000186"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8385505.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30995..32890)
                     /locus_tag="ACGE4T_000187"
     CDS             complement(30995..32890)
                     /locus_tag="ACGE4T_000187"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8385506.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32948..34723)
                     /locus_tag="ACGE4T_000188"
     CDS             complement(32948..34723)
                     /locus_tag="ACGE4T_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8385507.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34773..36269)
                     /locus_tag="ACGE4T_000189"
     CDS             complement(34773..36269)
                     /locus_tag="ACGE4T_000189"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8385508.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(36463..38085)
                     /locus_tag="ACGE4T_000190"
     CDS             complement(36463..38085)
                     /locus_tag="ACGE4T_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8385509.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(38413..39726)
                     /locus_tag="ACGE4T_000191"
     CDS             complement(38413..39726)
                     /locus_tag="ACGE4T_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8385510.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39971..40195)
                     /locus_tag="ACGE4T_000192"
     CDS             complement(39971..40195)
                     /locus_tag="ACGE4T_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385511.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(40215..40637)
                     /locus_tag="ACGE4T_000193"
     CDS             complement(40215..40637)
                     /locus_tag="ACGE4T_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8385512.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
BASE COUNT        11508 a         8697 c         6947 g        13611 t
ORIGIN      
        1 attttatcgt cagtttcggc ttttttatgc tttatttctt cttcggtgcg attcccttga
       61 tcaggacttc tgtccattct gctgtatatg gaagcagttt tgcgtatatc tccggcttag
      121 aaattccttc taccattgct tgaaaataga ggatcaagaa ctcatccgaa tatttcaaat
      181 ccacttttcc ttctttccgc cctcgatgga aaagatcaag catgatactg aaactttctt
      241 gcgtatacgc atccatcgcg tcagaaaatt cttttttttc tttttttgta tagtatgtca
      301 tcaaatcgat ataaaattgt ttgttcactc gattgagata acggattttg ttttcactca
      361 tggcaatcag tgtttcttca aatggaagat tttccgcaat caacgctttc gcttcttgcc
      421 ctaaatcatt cataaatgca aaaaatactt cataaatcag cccttccttg ctgccaaaat
      481 atttaaaaat cgtcgcttta cttacctgag cgttctcagc taccttatcc attgtcactc
      541 cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc ttgtaaaatc tctttctttt
      601 tctcttgtgc tctcttttca aaaccgttca tttgtagagc ctcccgtaat tttaaacttt
      661 tataatatat atagttcaaa tttattctga ggtttattat aactatctat tgagaaagta
      721 tcaagctagc aattccttgt ttatgaattc attgacagaa atttataaaa agaatattat
      781 gtactaaaat aataaaaaaa gttttataaa aaggtataaa acctttttat taaactattt
      841 aatcttgttt agtatatatt tttagaaaaa ggagtggggt ttttatggca gaaatcgtca
      901 aagtcacaca tttgaaaaag agctttggtc gtttcgaagc tttaaaagat gtctctttca
      961 ccgtacattc aggtgaagtc gttggattta tcggaccaaa tggttctgga aaatccacga
     1021 cgattcggac acttttaggg attataaaaa aagacggcgg aacggcaacg atttttgggc
     1081 aagatgtatg ggaagatagt ttatctatcc atgaacgtct ttcttatgtt ccaggcgata
     1141 tctctttgtg gggaaatttg actggaggag cgatcatcga tctcttcatg aagcttcatg
     1201 gaaaagggag tgctcaaaaa aaagatgaac tgattcaacg tttctctctt gatcctaaga
     1261 aaaaagcaaa aaactattca aaaggaaatc gccaaaaagt gggcttgatt gctgctttgg
     1321 ctgttgattc ggatctctat ttatttgatg agcctacttc tggtttagat cctttgatgg
     1381 aaacgatttt ccaagaagaa gttgaaaaat tgaaagaaca gggcaaatcc atcctacttt
     1441 cttctcatat tctcagtgaa gtcgagcgac tagcggataa agtcgtgatc atacgcgaag
     1501 gaacactcga tgaagaagga acactcgatg aattgcgtca tttgactcgt tcgaccatca
     1561 ctatagaaac agaagcagag aatagtcact tagcacaatt gaacggtgtc catgactttg
     1621 tgcaaaaagg tcacctcgct caatttgctg ctgataacga agctttcgat gtgattttag
     1681 ctgaagcgtc caaattaggt gtgaaaaaat ttgaatctat tccaccgact cttgaggatt
     1741 tattcatgag acattatgaa ggatgaggag gaagcgaaat gactgataaa ttttcacgct
     1801 ggtccaccct tttttctcaa aaccttagcc gtgactggaa aaagatcctg atatggctag
     1861 tcggtgtcac tgctttttct ggtggttttg tccctgcgtt tgaagaattg acttccggac
     1921 aaggattagc tggattatac gaaaccttgc aaaacccggc aatgattgca atggtcggac
     1981 caactccagc ttcgaatgct gctgagtata ctttaggagc gatgtatgca aatgaaatgt
     2041 tgcttttttg cagtttgttt gctgctgtga tctctgtttt acacgtggtg agccaaacga
     2101 gaaaaaaaga agaacttggt ctgattgaac ttattcgttc ctttcgagtc ggacgtcaag
     2161 caaactcttt agctgtcatc gctgaaagta ttctgatcaa tttgctttta gcaatatcga
     2221 tcagctgtac gatgattttt ttccaagcgg attcgatcac tgcaaaaggt gcttggcttt
     2281 ttgggctatc tgtaggtagt atggggattt tggggagtgc cgttgcactg tttgctgcac
     2341 aactgatgcc aagttcagcc agttcgaatg gtctgtcttt agcgttttta ggtggacttt
     2401 accttctacg agcaataaca gatgtagctg attcctccct ttcgatgtgg aatcctttag
     2461 gctggtctta tttgacctat ccatttacaa ctaacaactg gattcctttg ctttatttgt
     2521 ttttgttcag tctaatcttg ataatcagtt catttgtttt agaggaacat cgagatttga
     2581 acaccggcta tatccccgaa cgtgaaggaa aaccgtatgc taaacgttct cttttatcct
     2641 tgcgtggttt attattctat ctaaataaag ggatgacaat cagttggctt atcacttttg
     2701 gtttgatggg ggcggcatac ggctctatct acgggaatat gcaacagttt ttagaaagta
     2761 acgacttaat gaaacagatg tttatccaat ctggcgtctc aattgaaaaa aactttacaa
     2821 gcacgatcat ggtagtcttg atttgtctag ctgctatttt accaattgct agcatcaatc
     2881 gtttatttac agaagaaacc cacggacgta tgaatcagct gttcgcaaca aaaatttcta
     2941 ggaaccaact ttattggacc aatatgttac tagctattct atttgctata ttggcaatcc
     3001 tgattagttc tggtgcacta gggctttctg ctttggcttc gatgaaggat gcgaaagaca
     3061 tggatctatt gatgtttctt aaagccgggt tcaatttcct gcctgccgtt ttagtgttta
     3121 tcggaattag tggctttcta ttcggatggc ttcctcgaat cggtaaactt gcttatatct
     3181 acttagctta ctcctttgca ctcaattact ttggcggaat tttggattta ccagaatggt
     3241 tttccaaaac agctgtccct agctgggttc cacggatgcc aaacgattct tttgatcttt
     3301 ccgtgttcct tacgttatca tgtatcagcc ttgcactgtt cgtccttggt tctattggtt
     3361 ataggcgaag agacttacta gaaggataat aaaaaagcat gtacaatttc atgctagcct
     3421 ccaaatctgg gtcaaaaatc caacatttgg aggtcatttt tttatttaca agtcttctaa
     3481 ctgctcttta tcaatgatcc agacttctcg attctttctt tttacataac cattctcctc
     3541 taataattta aattttctag ataaagtttc gggggtcgta ccgataaatg cagcaagttc
     3601 tttcattttc attggcagga aaaagtgatc cttccctgtc gctttagcta agtcaagcag
     3661 atagacagcg actcgttctt ctactttttc catcatcaaa aaagttgtct gctgctgtac
     3721 ttttgttaat ttctcaacgt tgattgctaa aagtttcaga ctaagggcgg gataatgcag
     3781 taagagttcc gagaaatctt tttgtctcaa tatgcaaatt tccgtttttt ctagtgcttc
     3841 gccaaataaa gactcatttg gaacgcctag caactggctc tctccctcat atccgcccgg
     3901 ctctattact cgtaaaagct gttcctttcc gctagcagat aattgataca ctttcatact
     3961 gccggaggcc acgatcacta attgaggttc tccattcggc gcaagaatct gttctccttt
     4021 ttccacatag tgatgtttga caagtgcgtg tactttttct tgatcttgct gtactaagtg
     4081 attgaacaaa ggaacaaatg aaacacataa atgatcatga ctcataatct ctcctccttc
     4141 taaacagcta tttctaataa agaaacataa cttcccttat tccaactgat aattttcttt
     4201 tatcatagca taaacgtcaa gcgaaccaga taccagcaaa tagagtggat ttctttttgt
     4261 cccttgcttt attttccttc ttttgagacc ttaagtaatt gattcctatc aattttttaa
     4321 atatttactt tttgaataat caaaaacaaa ccttcactcg caagccgaga gtaaggtttg
     4381 tttttcgcat tgcttatttc gtgttgtcta ttgaaatttt tccgcaccta gcttttgata
     4441 tagtggtgct agttcttgta aggtttcagc ggttatttgc tcaacgtgtt cgatttcttg
     4501 cggatagaag aatagtttac ccatttgaaa ggttcggtct ctcatcgctt ttttcaactc
     4561 ttcttgtttc tttgtatcgg acaaatagtc agacaaagct aagtggtcgt caagttcatg
     4621 ttctggttgc ggccatatgt aataatcctt gatatcgact gttttgctcc actctttcag
     4681 tatcgcaagc aactggttat acgctgcaat gctaccatat cgttcttcgc ttttataatg
     4741 ttggtacatc aaatagattt ggtattgttt ttgattcaac aaaactgcta gacaggcatt
     4801 ctcgtcttga cgagattcgc ttcggtacgc gctccaaaaa tgactgcgta aattccagcc
     4861 attcgtccag ctttcgattt taggtttgct catgccgtac atatcaggca aaagtgcagc
     4921 tgtctggagc tgaatctctt tccatttttg ccagtgttct ttatattctt tcttgatttg
     4981 ttcaatctta tctgttggat ggctctcttt gagctgtttg aatgcgtaga ttggtcgatc
     5041 aaacaactga taatctgatt ttccaagttc cattgtttcg cctctttccg ttatcttttg
     5101 tattctacct gaattttgcg atatggacaa atagaggttg tgaaaaagct gttctgcatc
     5161 aagtcataag aacagccaaa caaaaaactg cgatgagcat caccgcagtt ttattgaact
     5221 taaggttgat tgatcacatc taaaagcgtt gaaaataaaa tggcactaga agcagctcct
     5281 gggtcgatat gtccgattgc acgctctcct agataggaag ctcgtccttt tttagctacc
     5341 aagactttgg tgtgttcttt tgcctggtca atgatttctt ttgttaaatt ccctgccttc
     5401 aatgcctctg tgactggcag ccaaacatct aacatcgttt tatcgtcagc ttctgccttt
     5461 cctcggcttt gaatacccgc tgttccctgc tcgatcactt cgcctaattt ttcttgagaa
     5521 tcaatagaat cgacctcttt tgtcgctttc atcatgttca tgaatgcaga tccgtataac
     5581 ggaccagaag ccccaccgac tttcgatatc atcgccatag agaatacttt gaatgtatcg
     5641 ctgattgttt caggatgatc ggcttgaaaa gcttcttcat aagctgaaac ccctcttccc
     5701 atgttatttc catggtctcc atcgccaatc ggggtatcta gatcactgag atattgtttg
     5761 ttctgattga ttgcctctgc aaattgcgtg agccattctt ggatttcttg aacggttagt
     5821 ttcatattat caccctttta tcttgagatt ttttaccatg aaatcgtctg tacattgctt
     5881 tctaaagaat ctttccattg tgtctccgtc aagtcgatca gtgttagaga aagaccttgc
     5941 atatctagtg aggtcataaa attaccgact tttcggaaaa ctacattgac gcctctttct
     6001 tccaacagag ctaaaacatc attcatgaac acgaactgtt ccatcaaagg cgtcccgccc
     6061 attccgttga ctagtacacc tgcttctttt ggtaaattcg agtaatcatc taaaatctta
     6121 gtaacaagtt cctttgccag tactttagaa ggctgcatct tttctcgacg atagcctggc
     6181 tcaccgtgga taccaacacc atactcgatc tcatcttctc ctagttcgaa tcctggtttc
     6241 cctacttctg gtacagttgc tgctcgaagc gctagaccga tcgtcttagt tgcctgtacg
     6301 accttttctc ccagttcttt caactcagct agggaagcac cgtttcttgc tgcatcgccc
     6361 acgattttgt gtacaaggac agtacctgca actccgcgtt ttccagcagt atacgtacta
     6421 ttttcaacgg cgatatcatc atctacgatc acactttcta cttcaatatc gtccatagca
     6481 gctaattcct ttgccatatc gaagttcaat gcatcccctg tataattctt aacgatcagc
     6541 aaaactcctt ttccactatc ggcttcctta atagccgtct gaatctgatc tggagttgga
     6601 gaagtaaaca catcaccaag caccgctgca cttaacatcc cttcccctac aaacccggca
     6661 tgggatggtt catgtccgct tcctccacca gaaaccaaac caacttgttc cgctttctga
     6721 ttcttggcta caacacggga agattctaca cgatgaacaa gttctggata gctttttact
     6781 aatccttcca gcatttcttc gacaatattg cttggatcat tcatgatttt tttcaattta
     6841 ttcccatcct ttctttccgg cattttgtgc ttctgttaag atttgctgag tatcatctgt
     6901 tataccggca gtgatggctg ctgcaaaagc tccttccact aaaggcgcat ccaccagatg
     6961 ataacgttgc tgctgctctt cttccaacat gtcttttgcc agttccgagt tcaataccgc
     7021 gctaccgata tccgcaaaaa caaaataact agctgcatcg cttgattcat tgactgcttc
     7081 tacgattttc atcgggtctg atccgatctc gccatttatt ccgccgagag aaaaaatttg
     7141 cacctcttcg gatttttgca tctgctcaat catttcttta ataccatctg ttactttttg
     7201 actatgagaa ataagcaaaa tacttttttt cacaagaatc ttcctttctt ttacatagtt
     7261 gctaacgatt ggtttcgctt tcattaatga acaattcaag tatataataa atttctaatt
     7321 ttgggaaaat atgtgtagtg cttcttgaag tttgttatac tttaattaaa agagaggagt
     7381 gatcgaatga ttacaacaac aacgaacagt gtagagggaa aagccgtagt agcttataaa
     7441 gggatcgttt ttggtgaggt cattactggg atccatgcat ttaaagactt ggaggcgggg
     7501 ttgcgaaata tctttggcgg ccgctcaaaa agctatgaaa acgaactaat gggtgcaaga
     7561 gaagaagcac ttgccgaaat ggccgaacgt gcaaaagcat taggagctga tgcgatcatt
     7621 ggcgtaaaaa tggactatga agtattaggc agcgacaatg gcatgctgat ggtcacatgt
     7681 agtggaacag ctgtctccac gagataagta ggggacatct gttttaataa ctaaagagag
     7741 atgggacatg tagtggcact caagaattag atttttcagt ctaacttcga ggtactctac
     7801 cttcccatcc tctctttttt atttcttttt gcggatgatt ccgtaagctg ctgcgactaa
     7861 cacacccgcc cctgctaagg aataactgac agaagctgtt ttttcatttg tttttggcaa
     7921 agtctgactt gttgatttgg atggctctgt cgtttgctga actgtttctg tcgtgcttcc
     7981 agaggtcact gatttttctc ctgccgaatg caaaatatct tttattcgtt ggaaactagc
     8041 tgctaagtca gctttttttg cttcaaaatc attagcaact gtttgtgctt cgctgatctt
     8101 tccgtggatt gcttgactgg ccgctgtgat atccgcttga gtttgactta gctttccaga
     8161 agctgtttga gcaacctgat ttaattgctg attgaattct tttgcttgtt ggatcttccc
     8221 gtcaagatct gtttggacag cttccgcttt tgctttcgct tcggcaagct ttttctcaag
     8281 ttgtgcacgg tcatttccca ctttattttt aagatcagct aaatcttgat cgattgctgc
     8341 ttggtcttct ttcaatttgt tttttgcagc ttctaatgct tcttcgagtt cttttctttt
     8401 attttccaag tcttgcaaat gatcgatttg gtcagaagag gtagaactat ttattgatgt
     8461 atcttctgaa gatgaaatcg tttctcctga attatctgtc ggtacattgg aagtcatttc
     8521 tgtttccgag gcaccgtttt cagcaccaga agagcttatg gttgttgaag gttctgtttg
     8581 agcaggttca gaagtttccg caaaagctgt ctgtcccaat aagaaaatac tggaaacagc
     8641 aattggccag attaacggct tcattctttt tttcatcact tttcccatcc ttactacttt
     8701 ttaactaatt tcttattata agcaaaaatg tttggaagaa ccataaacac aacaggctgt
     8761 ttgtctaaaa aacggtgcat cttttatttt tttgtacgtt ccgggctttt ccatgtcccg
     8821 cttccataac ctaaaatcgt tttgactgct ggaatgaaag tcgtcacgat cgttatcgcg
     8881 ttcactatcc agaacaaaag catatatagt ggtgcgaaaa gaaggtattt caattttctt
     8941 cgtcgatcat ctacaatcag cgaagctagc aactggaaaa atcctgcgac catttcaaag
     9001 cagatgaaca caaaactgaa tgtaaaagta atataaatct gttcgtagtt tccttgatat
     9061 acataatgcc cgattaatcc agcaaaaaga actacggaaa tacaaaagaa tattgaccag
     9121 acaatactca atgtttgatc aatgaagatg atcgttcttc caatatgttt aaaaggatgg
     9181 agcatcactt ttttgaaatt cgtcagccag acttctgttc caccttttgc ccaacgtttt
     9241 ctgtgtcgat atagcatctt caacgtcaca ggtacttcca tgaaaaagat aatctcagga
     9301 gcaaaaacag ataaccagtc atttaactga tgatcccaag caatactgat gtcttctgtc
     9361 gctcgatctt gtctgaatcc gcccacatcg atcaaggctt cttttcgata catcgtattt
     9421 gctccactgt acgcataaag accaccgaaa acagctgact gtgttcgttt aatgatgccc
     9481 acgatacttg agaattcaac cgtttgagat tttgcaatca atttggtacg attttggaca
     9541 tccatatttg cagtgaccgc agcaatatgt cttgcaccag ggcgaatgaa ataattcaca
     9601 tacctaatca gtgcatctgg ctccggtacc gtgtctgcat cattgcttaa aatcaatttc
     9661 ccttttgcaa aggcaagtcc gatattaaac gcgtgggctt ttcctttatt tttttcgatc
     9721 cgaaccacac gaagattagc gtattttttc atcagtctag ctaatatttc tggtgtttgg
     9781 tccgttgaac catcgtctgt cactaatacc tcatagttgt gataattcaa tttcgtcatc
     9841 aaatactcga ttgtatattc gatcacaatt tcttcattat gggctggcac catgatcgtg
     9901 atgaacggtt cgacactcaa gggaacatct acccattctt tttgcttatg tttgtataaa
     9961 aacacataac accagacacc aataaaccat acaaatccgc ctaaaatcgg ataagaaacg
    10021 agaaaaaaat acataataga caaacaagta tctaagaata aattacctgt atgggtgatt
    10081 gagatcattt cagttccacc ccattttctt tatacaaatc tgcaatgaat gtcgtatcta
    10141 gatttttctc ttcggagaca gaataaaaac ggaccgtttc tctctcttct tttgttccaa
    10201 aacgttccgt atacacttct tcaagtaatt cttgtctttt tttcaatttt tcatcttcat
    10261 attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta gtcaatacaa
    10321 atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag aaatacagca
    10381 ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta
    10441 atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa agagccacta
    10501 acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct ttcaccaaaa
    10561 ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt tttttgttac
    10621 gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct aaatttttcc
    10681 taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt ctttttatca
    10741 gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg acaatgcttt
    10801 tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca tctccatcgt
    10861 tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga ctcaaaggaa
    10921 tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt agcgtttggg
    10981 tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa accaaaatct
    11041 cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg ggttcttttc
    11101 cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag gttttgaaaa
    11161 gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga tggatcggca
    11221 gatggtagtc gtcgaaacaa cgcataatat cagcaaaagt agtggaagaa ttaaagattg
    11281 cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg atcaatagac
    11341 ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga aatgctggta
    11401 catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct tcatcaacag
    11461 tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt ctttcaaaca
    11521 actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt cgatattttt
    11581 tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca gaaatcaatt
    11641 taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc tgcacagcag
    11701 gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc tttttcgttg
    11761 cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt ttttcgatcg
    11821 tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa ttcggatgat
    11881 ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta cgcaaacgtt
    11941 ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct tcaccttcta
    12001 tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt ggatttcttt
    12061 cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa
    12121 tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac gcaatacgct
    12181 gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata ttttgacttt
    12241 tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata gccttcattt
    12301 cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata gctacagacg
    12361 tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa
    12421 ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg ttattgtaaa
    12481 aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca tcatctgtga
    12541 aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag tctacaacga
    12601 cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga ttttctgcgt
    12661 ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc atcgaaaaag
    12721 tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca
    12781 acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg ttttttttat
    12841 caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt tgtgtgttga
    12901 ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc tcttttaaga
    12961 gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc ttcaggtcgg
    13021 acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc agactgcttc
    13081 aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat atattcttgt
    13141 tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg tttggcttcc
    13201 ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaaat ttttcctttt
    13261 gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc tgcaatgttc
    13321 attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg cggtgcatac
    13381 gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa
    13441 atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata aatctcgcca
    13501 gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata atagcttgca
    13561 tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt ggctgttctt
    13621 tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc tgttccaata
    13681 ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct accttgacga
    13741 ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg ttttacaacg
    13801 atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc gtctactagg
    13861 atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt atcaggacga
    13921 acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc cagtcgcaat
    13981 cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc tactccttga
    14041 tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg aagaatatgt
    14101 ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc tgccatttcc
    14161 aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc taatgcatgc
    14221 atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc ttctgttatc
    14281 cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc atcgtactgt
    14341 tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg aaagatggct
    14401 gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga ttctggcgtt
    14461 tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg caagccgcaa
    14521 tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc ttccattaat
    14581 cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat aacgattttc
    14641 atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac gtacattcac
    14701 tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca tccctgattg
    14761 acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac tttgtaatgg
    14821 aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc taacatcaag
    14881 taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg attttattga
    14941 ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac ctcatggggt
    15001 atattaaaca ggagctttat taatatgtca accagaatga ctcttcattt ttttcactac
    15061 tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa atagaggaga
    15121 atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca
    15181 aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca
    15241 attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct
    15301 gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca
    15361 agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata
    15421 ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt
    15481 ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact
    15541 ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta
    15601 tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag
    15661 gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag
    15721 tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct
    15781 acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtaataaga
    15841 acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg
    15901 caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt
    15961 agagaaaaaa acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag
    16021 gtcagctgat tgaacaccgt ttaaggaaga gattgtgaaa aagtcgctta gtttcgagta
    16081 ttaagaaaaa ttttgtgaaa atggatttct acatttttgc ataaatttgg cttaataccg
    16141 agaaactggc ttttgaatac cgtttaagga agaggttgta aaatcagcgg tttgacctga
    16201 gtattagaga aacaaacgga aaaatagctg ctcatatttt gccgtttttt gagctaatac
    16261 cgaaggtcag ctgattgaac accgtttacc aagaggttgt gagcctacca tttaactccg
    16321 aatcacaaga aataatttca aaaacaactt ctcctgtttt tggttcggtt ggcttattat
    16381 cgagaagtag ctactgggag tctatttatc aaaaaaagga atgcgacata ggtcacaccc
    16441 cttttttatt ttctttgaaa catctttcgg tactcgacag catacaagac aaaagattgt
    16501 aaaagaccgt aagtgatgac ggcaaaaatg actctcaccc acagcgttgg taattctggg
    16561 aaaagcagca aaaggatcat gccaacatag atgacagcag tcgctacttt gccataccac
    16621 ttcgctccac ttaatttgat atttttagat agcccgatta atccagctac aaacataaag
    16681 ccttctttta tcaagaatag accaaacaac agccaaagcc acggtcgata ccatgccata
    16741 gacaaaatca gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac cttccctaag
    16801 tctgttacct gattatatct ccgagcaatg accccgtcta gataatcaga caaacctgag
    16861 acaacaatga ccgctcctgc tatataaaat gactgtctca gcacaaaata aagataaatc
    16921 ggcagcaaaa aaatacggaa tatcgataaa agattcggaa tcgtttgtat ttctttacgc
    16981 cagtctgtgc ttaatttcat tttatcgccc tacctcctca gcactctttt ctttttattt
    17041 tagcttacac ggggaataaa accaatgatt tcactgtttt tatttttctt tacagacact
    17101 gatattatga gctaatttga gataatcact tgctgttttc atcgtttact cttctttctt
    17161 cctgtcgttt gcgcatttct tttactagta ctttatgagc tttttttgat cctcgctcta
    17221 ggtcacgctg ccgtttacgt gttttataat cgataaacag actgtctaaa tcgtaacctg
    17281 tcgggtacag atcttctgct gaacgcacca gacgaacacg ctgacgaggc acgtggatta
    17341 tttttttgtc taaatataca tcaatcgtat cttcacctat atcttcataa actaacgctt
    17401 ctttttggta ttcattgaca aatatccgat caccttttgc aaagagctga cgttcttttt
    17461 tattctcctt cctatttgta actgctgtct taggaagaaa ttcttttttc tgtgtccgat
    17521 aggctttttc cgccaagtaa cgctctgctt gatggatcaa tttcatcgac atatccattt
    17581 tatgagcaat ccacaaagct tggctttctc ctgtttcttc caacccgcaa ctgatattta
    17641 ggacgaagtg cttcccgatc gaaagccata gcagccggca caaaatcttc atgatctccg
    17701 gcgaatttct tgatttctcc ataatgggtc gtggcaatga tcaatgcccc ttgctcatac
    17761 atagattcca tgattgcgat cgctagtgca gccccttcat ttggctctgt tccactgcca
    17821 atctcatcta aaagtaccaa cgtattcctt tttatctttt tcaaaatagc agcgatgttt
    17881 tgcatatgac cagaaaatgt actcaacgca ttttctaaat tttggtggtc accgacatcg
    17941 acgaaaatct catcaaagac agcaagctct gtcccttctt ttgccggaac ttgcataccg
    18001 aacatcgcca tcaaagtcaa taaaccgact gtcttcaaca cgactgtttt gcctccggca
    18061 tttgctccgg tgataatcaa gcctcgatag tctttaccga tttcaacatc caatggtacc
    18121 gcatgctcag gtaaaaatgg atgtctgcct tgcttgataa tgatatgttc cgatttattg
    18181 attctaggtg tgcttccatt gatttctcgg ctaaattttg cccgagcaaa gatgatatct
    18241 aacaccgtga tcgtttcgat caataaatcg atcacttctt cgttttctgc gatagcacca
    18301 gtcaatgccg ctaggacttg atattcttca ctgatttctt cagcttttaa caactgatag
    18361 tgttcattca atttagaaat cacagttggt tcaataaaaa ccgtcgttct tttatttgat
    18421 tcatcgataa tcgttccatc tattttattt ttatagctgg ctttgatagg aatcgtatag
    18481 tattcgcctt tttgaacaat cattgcttct tggatcattt ctttgttttt cggatgacgc
    18541 aagaatttca gtaattttga ctggatttct ttttcgatta gttgtaattg tttgcgtact
    18601 ttacgcaaat tacgtgacgc gtcgtcactg actttattat tttttatttt ctggtcaatc
    18661 aactcttcta catttatcaa atcaggaaga tgtttgctat aagcaaacaa caagggtgtc
    18721 tggtattgat tcttatcaaa aaactttgtg atcatccgac tgcttctaag aaaatccgca
    18781 tattcgatca aatcggttgg ctgcagaatc aacccttttt tgacttgttc tgtcagtgca
    18841 tcgatcctcg gcaaccccat aaatggaaca tgctgactgc tttctaagat caagcgtgct
    18901 tctcttgttt cttcttgacg atccaagaca gtttgtaggt ttgtcgagac cgtcatatcg
    18961 ctgattcgtt ttttgctata gtttccaatt gctcttgctt ggatttcctt tttgatccga
    19021 tcaaattgta attgactaat catttcgttg tccatattct ctccttttct tgaagagata
    19081 cctgtcccaa aataaataag ggcctagaga taaataaaag gatagaacac agcttaaaaa
    19141 agtcatgtcc tatccttatt agaatacaaa gatattgttt tcctgacagg taccgtttca
    19201 taagcacacc aaataaactt tgtttattgg ctattcgact tactgattag ataccagcta
    19261 cactcacaaa acatacccca ttcttctagg tcttatttat tttccactta actatagcaa
    19321 gacacatttt ggttgtcaaa ctttagtatg ttttatagat ttacaccatt aatgaataga
    19381 cacctaccgc gataattgct ccgatcattg gacctgcgat tggtacccat gaatatgccc
    19441 aatctgaagt tcctttatta gctattggca ataccgcatg tgctaatcga ggaccaaagt
    19501 cccgagcagg gttgattgca tatcctgttg gccctcctaa tgaaagaccg atggctaaga
    19561 tcaatccacc tacagcgaat acgttcgttc caggtgcaaa ttcgttttgt ccaaaagcta
    19621 gcaacccaag aactaataca aatgtaccaa ttaattctgt gatcacattt gctggataat
    19681 tccgtatagc aggacctgtt gcaaacgttc ctaaaatcgc gctttcatct ttcgttgcat
    19741 tccaatgtgg caaataagaa agccaaacaa gaatggcccc tgcaaaagct cctaaaactt
    19801 gcgcaatgat gaaaggaacg acaagattcc aactaattgc ccctgtcatc gccatagcta
    19861 gagagactgc aggattcaaa tgagctgggc ccatgaatcc agaaatatag acagccattg
    19921 ttacagcgag tccccaacca aatgcgatga caatccagcc agaagcctgt gctttacttt
    19981 tgtttaaatt cacagccgca cacacaccat ctccaagcaa aactaggatc agtgtcccaa
    20041 taaactcccc catgatttga gtcatatcac tgttcattta tttctccccc tttagttccc
    20101 gcagatctga ttctgcaatc aatgcttcta aacgtttagt ctgttgcgcc ttctcctctt
    20161 ctgtccattc aaaatatcca gcaattgcat cgatcacagg ttgcttgatc tcatcaagct
    20221 gatctcgttc aaacaacaga tgatttgtac gacgaatgag ataatcgcct ggggccaaaa
    20281 ccatttcttc ttctaaacca taacgtaacc gagctgactc agcgagactc aaaccaggat
    20341 aaggtgccat ttcttttgct aattcaaaga tacgtcgggc attagtcccg taaaaatcag
    20401 cgatataagt agcatcttct tcctctaaac cggctgctac tccttctttt gctaattctg
    20461 tcactgtttc ttctaattta gttggatcga agtttccacc tgaaatctga tattttttag
    20521 agtcgatttc tttcgtctct attccgtatt cttcttttaa taattggcgt atcaatcgca
    20581 aagctccttc tgccatctta cggtaatcgg tgattttccc accagataaa gtcagcaaac
    20641 catctggttc tctttctaaa gaactacctc tggaaatcgt agaaggtgct ttttcatcac
    20701 ggctgttttc caaatggttc aacacgtctt ctacttcagc acgagaaact ttgttttcct
    20761 tatattcact tactgtatcg acaactttat tgaagctctt gtctgaaatc gatccattat
    20821 ctccaccatt gtaatcagaa ccagaattac caatcaacag tggacgaagt cctgcccagc
    20881 ttgcttcgat atcattcaat gtgatatttg cttctggata gcgatgattg atcacatcca
    20941 atagataatc tacatcttct tgtgtgactt tcggatccgt aaaatctcct tggtaatccg
    21001 tatctgtcgt accaaagtaa gtcttgtttt ctcttggaat agcaaaaacc atccgcccat
    21061 cttgctttcc tgtatcgaag tatgtgggtt gcggtacagg cagttttttc gcatctacaa
    21121 ctaaatggat ccctttagtt ggacgcattt taggagagac tggacgcgta aaattcaagt
    21181 ttcgtacttt atctacccaa ggaccactcg tattgatcac taattttgct ttgatttcga
    21241 tcacttcatc tgtcagcagg tcacgagctt tgacgccaac gatttgatcg ccatcatata
    21301 aaaagccaac cgctttcatt ttacttacta gataagcccc atcttcggca gcctttttga
    21361 tattatcaat cactaaacgg gcatcattgt tgcggaaatc tagatagaca cccgcgcctt
    21421 ttagcccttc ttttttcaaa aacggttctc tttccaatac ttcttctggt gtcagcgtat
    21481 aattctcata tttggttcct gtcacatttg ccagtttgtc ataaaggtcc attgctactt
    21541 tgaccgagaa catattgaag gttgttgctc cttcgccctc atagattggc aaaagcattg
    21601 gatctggttt tgggatgtgt ggggcaatac tttgtacgac tgcacgttca ccaactgtgt
    21661 cagctactac ttccacatca aatgtcttca gataacgaat accgccatga acaagtttgg
    21721 tcgagcgaga ggaagtccct tctgcaaaat cctgcatttc gatcaatcct gttttgattc
    21781 ctgatgctgc tgactggact gccacaccgg caccagtgat tccgccaccg atgatcaaca
    21841 gatccaattc ttcttgctgc attttttgaa tgctatcttg tcttgtcttg tttgaaaaca
    21901 tatggatttc ctccttattc aaatgcttgg gtagctttta ctgctttttt ccaaccgttg
    21961 taaagttttt ctcggcgctc ttcctccatt tgaacttcaa atagcttgcc agcttcataa
    22021 aattcgcgga tctcgtctgt atctttccaa taaccgacag ctaatcctgc taaaaatgct
    22081 gcccctagcg ccgttgtttc caagttttct gctcgttgga tcggtacatt caagatatct
    22141 gtttggaatt gcatcagata ttcattgttt gctgcaccgc catctacttt caatacagga
    22201 atcttgattc cagtgtcatc ttgcatcgta tcgatgatat ctctcacttg ataagcaatc
    22261 gactgtaaag tagctttaat gatatcttct tttgtcgtac cacgtgtcaa gccaaacata
    22321 gcccctcgtg ctgcctgatc ccaataagga gcacctagcc caacgaatgc aggaacaaca
    22381 taaacctcat cttcgcttgt agagcgtttc gctgcgtctt ctgaatcgct agctttttgc
    22441 aacatttgaa gtccatctct cagccattga atcgaagaac cagccacaaa aatacttcct
    22501 tctaaagcat agtaaacctt tccatttatg ccgtaaccaa ttgtcgtcaa taaattattt
    22561 ttggataatt gcggtttttc accggtattc atgacaataa atgaacctgt accataagta
    22621 ttcttgacca tgcctggttc gaatgccatt tgtccgaata atgcggcttg ctggtctcct
    22681 gccatccctg aaattggtgc ttcgcttcca taaaaatgat aaccttgcgt ataaccataa
    22741 acttctgagt tagaagtgac ttttggtaac atcactcgag gaatatttag taaatctaaa
    22801 atctcctgat cccaatctag ctgatggata ttgaacaaca tcgtccgact ggcattcgag
    22861 taatcagtaa catgtgcttg tccatcggtc agtttccata ctaaccaaga atcgatcgtt
    22921 ccaaacatca attcgccttt ttcagctcgt tcttgcgcgc cttctacatg atcaagaatc
    22981 cagcgtatct tcgttgctga gaaataggca tcgatcacta accctgtttt ttcgtggaaa
    23041 aattcttgat agccttcatt ttttaagtta tctgcaattc cagctgactg tctggattgc
    23101 caaacaactg catgatagat tggacgtcct gtatcttttt cccatattac tgttgtttca
    23161 cgctggttcg ttatgccaat cccagcaatc tgatttggtt tgatccctga ttcgatgaac
    23221 gctccggcaa taactgactg tacagaattc cagatttcat ttgcatcatg ttccacccaa
    23281 ctttcttgcg ggaaatattg cgtgaactct ttttgagaac tgccgatgtg tcttcctttc
    23341 ttgtcaaaga taattgctcg tgagctagtt gtaccttggt caatcgccat gatatacgtt
    23401 gattctgtca ttgtgattcc ctccattata tgcaaacgct ttctttaact actcttatca
    23461 tactactgtt ttaaaaaaag aaactgtcaa aattttattc tttttttctt tttttgacag
    23521 ttttttatta tgaagctatt ttttaatcaa ttcttttctt ttttcagcgt ctctattctt
    23581 cttttcaatc gaataatgtc gtaaggtgac gcaaattctg acagataata ttcacactgt
    23641 tttttgtttc tctggcaaaa tgtgatcacc aaatcatatt ctttaccttc ttcataactt
    23701 ccaatctcaa taccgccgat tcccctcagt tctctgatca gatattgttg aaaagcaata
    23761 cgataaatcg gcagactttc taaatcgatt ccaatcacta ctggttttgc aatataaaaa
    23821 tcgatcatca aaagaacatt ggcatactgg atcatcaggt aactaagctc actatcttca
    23881 gctcttttcc tcggcaattg ctgcaccgct gtttcctgta atttcgccag taactgtgtt
    23941 aaggaatgcc cgagcatttt ctgctgctgt tccaaaagcc gatcacgctc atagacttcg
    24001 atctttccaa caaagaaaaa ccattcattg ttgatctggg aaatttggta gcctactgcc
    24061 ttttcttctt tgattttcaa acgacgagga cgataatgcc ataacatcgt ttcccgaatg
    24121 taagtatcta aaacagcggt tggtaacttt ttgctccgtg tcaaatcata ctgataaaat
    24181 atttcttcgt ctactatcga aaaagaaaca aaaaagctgt aaaacatcat tgtttcgtat
    24241 acattcagtt ctgctgctgt tcgactaaga tgcatcacga tgattggatc gattgcttga
    24301 taaagccggt ccgactgata gagtaatttc ttttctttca aagttgcgta cgtacttttt
    24361 tcattcaata gtcttttttt cgtaatcccg agccagcaag caatctgtgc ttctgctgat
    24421 gccgtaaacg ttgtttctaa tacacggtcc aatcctagaa tgaacggtct tttttctggt
    24481 gtattttgca aaaaaagcgg tcgctgatct tccgggatat attggaatag ttcataataa
    24541 aaatatcgta tctgtagctc ttctccgtat aattgaccgt ttttgatttg taatccaaat
    24601 tcctctaaga gctggttcaa ttctttgatc tttcgaaaaa cagaagactc actgatatta
    24661 aacgctgtcg ctaatcgaac gatggatact tgctgatgtt ctaataaata gacgagcatc
    24721 ttaaacttca agctttcctg caataaaaac aatagaattt tttccaaact tacatcttcg
    24781 tccaacacaa gcagcgcctg atactcattt cgctgtactt ctactttttt ttccatcatt
    24841 cggccaatca attcaatatc ttccaaatat tgatcaaacg aggcttttga cagttggaca
    24901 tgctctctca aaccattgat agaggctgtt cccccttgca gaacaaggtt tcgcaaaatc
    24961 aggatctgta actgctcttt tctttctaat aatgcttcaa tcttcatagg cagtattcgc
    25021 cacctttgac cacaccatat ctggcacacg gtattctggc tggaaaaata gctcatgttc
    25081 ttctaactgt tcaaacgtcc atccttcttc tactgcaatt gtcagttggt caagaaggtg
    25141 gaacatctct tttttgcttt ggagctgtgc tccaagaatc tttttttcag aatcaaatag
    25201 aaatttgagt ttaactttgc tttgatttat tgctgtaagc ttgtagtcta cgtatcctgt
    25261 ttcaattttt tggggataaa aaatgctctc ttcttctgtc aaacctacgc ttcctaagaa
    25321 atagccaaac cactgattgc cgactggacg ataggttccc tgatcctttg tctgtgagcc
    25381 actaattgtc ttgctgacat ttcgtgctgt acggacagcg ttatttacta gagaaacata
    25441 cattttttca tttgttggac gaaaagttac ttggatcgca tcgccaatcg cataaacgtc
    25501 tttttgagac gtttgcaagt agtcatctac ccagatcgtt ccatcatcat tcaatgttaa
    25561 ttgttcttcc cacatctgat tgtcgggacg agaatgatta gccagcaaaa caagatcata
    25621 gttctccttt cttttttccg tctctacaat agcggtttca ttttcggtca aagacacaac
    25681 ttgttcttct aaaaaaagtg aaagcccttg atttttcatt tcctttacta acggttctgt
    25741 catttcagga tctagataac gaaaaagaat tgactttcgg ctttcgtaaa gatggatttg
    25801 tttttttctt gatgccaacc cttcagctat ttcgattcct gcttgtcctg caccaataat
    25861 tgcaattttc tttgcattca tgatcttttg ctgaagagcc gttacgtttt gtatcttttt
    25921 aactgggtga ataagcgttg attctgcaga aatatttcga aacttttgat ttgaaccagt
    25981 cgctagaatc aaacgatcaa aatccaattt ttttcctcct gctaaaacga cttttttttc
    26041 tttcaatcct attactgtct tttctgtgta aacatggatt cgatagcggt cagtcagttc
    26101 ttctttcgtg atccaagaaa gttgtttttc actggtaacc tttcctttta atatcaaatt
    26161 gaacccgctg ggaatccatc ctaacttcga ttgcttttca atcaaatata tttcacttgt
    26221 tggcatcaat tggcgaagat aaatagccgc atgaatccct gcaaacgacc cgccgacaat
    26281 tacaatgttc atctgcattc ctctttctta taaggtgttc tactgctaac ttctcaatca
    26341 gaaaacacag tattcataac tcgctttcaa ccttatgata catagcgatg cagcagaatc
    26401 aaccctctcc atcataccgt gttctaggtc tgcttctcaa taatgtacta gtggacctaa
    26461 ttgaagagta tcatttctga agagatgttt caaaaaatgt actcttataa aagaaaaaaa
    26521 ctcgcctttt acgtacctct gtccaaatag gaaacagaaa agaacgaaaa gacgagtttt
    26581 agcgtgtgta atgtaatagt tagaatagac acatcattta tttttaattc tttaatgctt
    26641 caacaactgc ttcgcctaat tgatgagcag attgaggatt ttgccctgta agaagccgcc
    26701 catcaatttg aaccacttct gaaaaagctg ctccagactg atagtttgct cctttttctt
    26761 tcaaagcatc ttctgctaag aatggtactt cattcgttgt accatttgct tcctcttctt
    26821 cattactgaa cccagtcact gtacgtccag ctaaaataga ttttccagac tcatctgtaa
    26881 ctggcaacaa tcctacaaca ccgtgacaaa cagctgagac cagcccttca tttcgataga
    26941 tttgtgaagc gactttccca agatctattg cttctggaaa atcccacatt gttccgtggc
    27001 caccagcata ataaaccgct gcataatcat ttgcatccac atcatcagga cgtaaggtat
    27061 ttcccaaggc ccgatttctg aaatcttcat cttcataata ggcccaatcg atttcatcca
    27121 tattcaaaac actttgcggt tctaaaggta catatccgcc tttaggactg acaaaatctg
    27181 catcgatccc attttttact aatacatcat aaaaatgagt cagttcactt aaccacaaac
    27241 ctgtaggacg ctcgatcttt tcgtactttt ctacatttgt taatacgatc aatactttca
    27301 ttttcccatc ctctttctat aaaaattgcg ttattgataa cgtgctttca agaaattata
    27361 taatgggctt ggcaaataaa gctctttata tgcttcttca aaaggtttcg ttacaaattc
    27421 atcctcacgg ttttccaata atttctctgc ccagtcagga tcgacgatca tttgctgtcc
    27481 aattgctgca agttctgcat atttcaatac ctgctctaca tcttcgcgat tgcggatccc
    27541 accgacacca actaaaggga tctttccttg aatcgtgtta tggaccactt caagaatcga
    27601 tttatcgttg tatttatcag aacgagctat tcgatcatac atatttaatg aaacatgcag
    27661 ataatctagt tttgactcac gtaatcgttc aagaagccag atcgtgtctt caaaacggat
    27721 acctggtgtt tcatattctt ctggtgagaa acgataccct acgatgaatg gttttttagc
    27781 atattgttca accgctgaga aaaccgttgt taataattct tcaataaaac gataacgatt
    27841 ttccaagcta ccgccccatt gatcctctcg tctattcgaa tgcggagaga aaaattgttg
    27901 gatcaaatac gtatttgctc catgcaactc gattccatca aatcctgatt caatcgctct
    27961 acgtgtggct ttgccaaatg cttggattgt ttcaacgatt tcttcttcac tcattgctcg
    28021 tggtgtttcc gcatcaggac gttttgctgc aactgcactt gcagatacag gctgttctcc
    28081 agataaagtt gctcgacttg tcattcgtcc accgtggaaa atctgaataa tcgcttttgc
    28141 gccttctcct tggattgctt tcgctagctc acttaatcga ggaatcattt catcatgagc
    28201 gatgctgagt tcacctggcc agccttttcc gatatcttgg acatttgcag caccagtaat
    28261 aacagcacca agacctttag aacgttgttt ataataagtg atctcatctt cggtgatcac
    28321 tccattgaaa aagctgagtt gtgtcgtcat aggcgacagc atcaatcggt tcttcaattg
    28381 aactcctgaa ggtaaagtaa ttgattgatc aaattgtgac attgtcattc ctcccataat
    28441 tcgctctgat taacctatcg agaatcatct atcattaaac cactcgatta gttgtacatg
    28501 caactaattt gcttaaaaaa ttttattttt tttgtaactt atctgttgca tcattcattg
    28561 tctgggctaa ttctttactt gcttcatttc ccaaaactgc tttgtatgac tgatgaagtt
    28621 cttcccagct accatcgact tccttagcaa attggcgtcc ttgttctgta agataaacta
    28681 atgacaagcg accttcgcta actgttttga cgtatccttt atattctaat ttttcaacaa
    28741 aacgagcaat cgttgatggg gcggaataga gcatttgtgc aatatctttt tgtggtattc
    28801 catctttttc ttttaatatc aataaaataa aaccataagt tggtgccaga cctgtccttc
    28861 taaacgcttc atcagctaat ttattcaaca tgcgatctag ctttttcacg ctaaagaaaa
    28921 gacattcatc taacgctttt tcaattggct tttccatttc atcccctcca ttggcgtaca
    28981 tatcatagta tgatttttat tttttgtcaa aaaacacgga ataatgtccg cctcctctgt
    29041 ctagatttga aaaaaagaag tagccatctt caaagaattt cggcttgttt tcgacaaaac
    29101 tccttctgga acaccgtgaa tcagaggttg tgaaaaagtc gtttagtttt gagtatgaag
    29161 aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa taccgaaaaa
    29221 ctggcttttg aacaccgttt atatcagagg ttgtgaaaaa gccgtcctgc atcaagtaat
    29281 aagaacagcc aaacaaaaat agttcctcat attttgtcgt tttttgagct aatgccgatg
    29341 gtcagctgat tgtacaccgt ttattgagag gtcgtgagcc tgccgttgaa ctccgagtca
    29401 caaggagcaa tttcaaaaaa cagcttctca tgttttatga ctatggtgac ttgtgatcga
    29461 ggagttggca ggagaatact gtttattcgt aaagaagggg ctgtgacaag acttttgtca
    29521 cagtccctta tttcctttta ttataggatt tcgccatttg attggatgac ttgtctgtac
    29581 caatcaaatg attttttctt taaccgtttc tttgtgccat tcccttcgtc atcttgatca
    29641 acatagatat atccataacg ttttgacatt tgtgaagtag aagcgctgac gatatcaatg
    29701 cagccccacg acgtataacc aaataaatct actccatctt tgatagcttc cttcatctgc
    29761 tcaatatgct gcttcaaata gtcaatgcgg taatcgtctt gaacggttcc atcttcttct
    29821 actttgtcta tagctccgag tccgttttca gctatgaaca gtggtttttg atacctatca
    29881 tataattgat tcaaagcaat tctcaaaccc ttaggatcaa tctgccagcc ccattcactt
    29941 gatggaagat acggattttt aattcctcca gttattaagt ttccttggac cccttcccaa
    30001 ttttctggat gagcactgac tgcactagac atgtagtagc taaaagaaac aaaatcaaca
    30061 gggtaggctg ctaagatttc ttcatcacct acttctattt ttaagttgac acctttttct
    30121 tcaaacattc gttgcgtata cgctggataa tatcctcttg cttgaacatc actaaagaac
    30181 aaagaagttc tttgctgctc aattgttttt tgaatatcat ccggatcaca agaatacgga
    30241 acaagaagct gatagctgat catacaaccg acttgtgaac ctttgataat ttcatgacaa
    30301 gcttttgtag ctaaagcact tgctataaac tgatggtgtg caccttggta tttcgcttgt
    30361 tccaaatggg gatggttttc ttcaattact cctacgctaa caaaagggtg atgttttaca
    30421 cagttgatct cattgaaagt catccaatat tttactttat ttttatagcg tttgaatact
    30481 gtttcagcga aacgaacgta aaactgaata agctttcgat taggccatcc accatattct
    30541 gtaaccaaat ataacggcat ttcataatgg gaaagagaaa cgactggctc aatcccatat
    30601 tttagacatt cgtcaaacac ttgatcataa aaaaataatc ccgcttcatt gggtgtttct
    30661 tcatcaccat gaggaaaaat tcttgtccat gcgatagaca tccgatagca tttaaaaccc
    30721 atttcagcaa ataaacggat gtcttcttta aagtgatgat agaaatcatt tccgtgtctt
    30781 ttaggataat aatacttgct gttgggatcc tgcgccgctt ttacttgctg atgcgtcatc
    30841 atatgccatt gatctgtcca tttttcttta ggtaaatcgc tgtcgaatgt atacacatca
    30901 gacacagata atcctttttc atctacatcc catgcacctt cacattgatt ggccgctgtc
    30961 gctccgcccc ataaaaatcc ttctggaaat tttttcatat cgatccccct aaacttgcta
    31021 gttttcttgt ttgattattt ttcttatgac tagtaatgat atttttgaat ctactactat
    31081 ttgtaacaat aaccattgtg gtcggatcat atccctcttt gattaattct tgcaaatcta
    31141 cttccatcaa taaatcgcct ttacgtactt tttttccatc agatactgca ctttgaaagt
    31201 atttgccatt taaattcact gtatcaatac cgatatggat cagtatctct gctccttctt
    31261 cactgactac cccaattgca tgtttcgttg gaaaaactgt tgaaacttcc ccgtttacag
    31321 aactgtagat ttttccgtcc gttggaatga tcgcaaaccc ttctcctaaa gatccactag
    31381 caaatgttgg atcgtttact tcagataaat caattatttc acctgctgca ggagtactta
    31441 acactatatc ctttgaggat tcttcttcga tttcatcctt gtagccgaaa agaaaagtta
    31501 gcatacagga actgatcatc gctaagaaat aactagcaac tagccaaaca aatccttcac
    31561 caatataaat cgggaaagac aaaatacttc ttgtagcaac tgccaaagca ccggctcctg
    31621 cggcaccagc tactccacca aatatagctc cacatataca agcaatgtaa aatggtcgtt
    31681 tatatttaag agtcacacca taaacagcag gttcagtgat tccaaataat gctgtgataa
    31741 atgcagataa agataaactt tttactttct tatttttcgt tttcaaaaat accccaagta
    31801 ctgctcctgc ttgcgcagct acggcagtca tacatgctgg acctagcgta tcacgaccgt
    31861 agaaagaaag attgttggtc attactggta caagtcccca atgtaaccca aaaatcacta
    31921 acacttgcca aaatccgccg ataacagcac cagataaaac agggctaaag ttataaacag
    31981 ctaaatatcc agctgcaatc agctgacttg cataatctgc aattggacct acaactaata
    32041 aaataattgg gaccatcacg atgatacaaa tgaatggtgt caataaattt cgaatacttt
    32101 cgtgcaagct tttgcgaatc ttaggctcaa tataagaaag aacccataca gctaaaattg
    32161 ctggaataac cgtacttgta taccttgcta gaatcacggg catccctaga aatcgcaagg
    32221 ttatgctatc actatatgca ctgacaatcg taggataaac gagagctgct gcaataacca
    32281 tagcgatgaa acgatctgta ttgaactttt ttgcagcagt atatgccaaa aatataggca
    32341 agaaatagaa aaatgcatca gctgctgcat tcaaaatgat ataagttcct gattcagtag
    32401 ttaataaatt caaactcgta cataaggcga gtactccttt gatcaaacca cttcctgcca
    32461 agacgcctaa agtcggggca aaaacagcgg cgatcatgtc tataaaagag tttaaaatgc
    32521 cttttttctc agttcctctc tttccactag atgcatcatc ttctagtttc atttgtttgg
    32581 ataatacgtc atacacttca tttactgcat ttcctactac gacttgaaac attcctccag
    32641 cttcgactac agtgatcaca ccttctagtt gctctacctt ctccttatct gccttcttat
    32701 tatcttttag ggtaaagcgt aaccttgttg cacaatggta aacattgctg acattttctt
    32761 cgcctccgac aaatcggaca atatctttcg ctaattgtga atagtccatt tttacttccc
    32821 ccttgttaat taatgccgta gcaatccttc ttacaagata agtatagttc ttagaaaacg
    32881 gtttctccat tcaattagta ccccttctaa tagcgaacga aactgtacaa tagtgtgtat
    32941 ggtttactta gccgagcaaa tcactcactg cttgaaagaa aacaagtata tcgtttctct
    33001 tatagtcaaa ggagacaaaa aaaacacgct tgatcttcct ttgctgatgt ataagtggta
    33061 tctcaagctg atactctgtg atttttgttt ctacttggct tttgataaga cagcacaaca
    33121 aagtctcggc atttaacgga agcataacta ggtcagaact aggagagatt agttgctgaa
    33181 tggaatctat ctttttatgg atttttattt gattttcttc tttgtagtaa tgatcaagca
    33241 gttttgtttt ctttttttct tcaactatgg tcagcttttc acgcaaaatc tgataaatct
    33301 cccaagattc aaactcatct agaacactag gtagataaat caaatcatcc cactcaaaaa
    33361 taagctcttg ctcagtagtc aaaattgcat catattccgt tattttgttt gattgatttt
    33421 tgaaaagata cactggttct atccggcatt ctttgatcca catgttcata ctttgttgaa
    33481 tctgtttttt tagtgtattc aaaatactaa ttggctggtt actaacaatc agtatctccc
    33541 cttgttttcg aaaaggattc aacgcttcgg caagatacaa aacaaaataa gcgctttcag
    33601 ctaaattggg acgtaattga aaatgtattg ggaagaaacg ccgaattaaa taaatttcta
    33661 aaggataacg ggagatgatc ttttgtacaa aatgattcat ggtattatgt ccggcatcca
    33721 tcctcgtttt catttgtttg atatgggtta gcaaaaattc tgtttcatgg aacaataacg
    33781 aaacaggaag atctaaaaaa cgaataatcg tttgctcaaa tgctcttaga ttcttctcta
    33841 tttctttgtt tttttcatca ttaatcatca aataatttaa ctctaatagt cgattttcaa
    33901 ttgcttgttt ttcttgattg gaaaaagaat agggtagttc tgatgataac ttttttgtga
    33961 gggtctctac catcggaata aataatgtat acctgtttgt tttttctaaa agttcttctc
    34021 ttcgaagaat ggtcaaaacg agataccgag aaaatcgtat gaagtcctct cctgaaacaa
    34081 tataattata tgatataaga acctctttta atgtcttttg gacagtcggc atgatattgg
    34141 aaaaaattgt actgatttgt tgaggaaacc ttgcgtgttg gatcaccgtt tctgttccaa
    34201 caagagataa gaaaattctt tttgaatttt cttcagcaat gatttttagt ccttttgtcg
    34261 acgaaacttc cagttctaat ttaggattgc gctcgatcca acgcaaaatc gtttttattt
    34321 gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc tatcttttgc atactgacat
    34381 accctttttg aaacaataag tacgtcacaa tagttgaagc tcggaaatcc atgctggaat
    34441 agattttatg tttcgtcacc tcttgtatga tttgctgttt agtttcagat tcaattgatt
    34501 ctaaaaaata acctgaacgt ttatttgact ggataatgat tttcttctcg tagatagcaa
    34561 gcatattttt gatttcagtt tggatcgttt tcttgttggt acctaaagct tcagctaatt
    34621 cttcgcttgt tatccaatga ttcttatttc ttaatactac aagaatttgg agttgtcgtt
    34681 tatttaagtc catttgctta cacctcctct ttgttataaa catacaggaa aaacctctca
    34741 aatgacaata gtcacttgca gaggtttaaa aatcatttga agaaagatct atattcaagg
    34801 tttttaccat tagactggat aatttgttta taatattcaa aactatcttt tttcaatctc
    34861 ctaaagcttc cgctcccatc attttgtcta tccacataaa taaagccata tcgcttcctc
    34921 atttcccctg ttcctgccga tacgatatcg attggtcccc accataagta accaatgatt
    34981 tggcaaccat cttttattgc ctcagcaatt tcctgaagat gtatttttag atactcacaa
    35041 cgttttggat cgtggattcg attcaattca tctggtgctt catctagtcc aatgccattt
    35101 tcaactatga ataatggtaa ttcataacgg tctgtcaatt cattcatgac ataacgaaac
    35161 cctagaggat caattggcca actccaaggt tcgggagatt tttccttcaa aaagggatta
    35221 tcgaaaccat ttgctccgcc agtatctacg cgcatttctg cttctttttt atagacagcg
    35281 ctacgataat aactaaatgc aatataatct gccgtgttag cttgcaagca ctgttcgtct
    35341 ccatctaaca ttactggtgc acagtcttct tccttccatg tcctgtaaat ataagctgga
    35401 taatgccctc ggcacatgac atcggagaaa aaccatgaat tgcgtttaaa ttgcagtgaa
    35461 ccaaatacat tctctggatc acagtcatat gcataagtcg ctaatgcaga aaagctacac
    35521 ataataccca tcttagcatt tggatcgatt ttctttaata attgagtagc acgggcgttc
    35581 gctagaaaca tatgatgaca ggcttgataa atcacttttt ttgttagtgt agcaatgggt
    35641 tcaagtttat ctctagcagg gaatgtaacc aatccagcag ctgcataagg catacgaaaa
    35701 gcattattga tctcattaaa ggtcatccaa tatttcactt tcccttggta gcgttcaaag
    35761 acggtctgcg cataaaattc aaaaaagtcg attaattttc gattttgcca ccccccatac
    35821 tctcctacca atgctaaggg ggtttcataa tgactaagag taattactgg ctcgatatca
    35881 tatcgattta atgtattgat tagtctgtca taaaatacga gaccagcctc atttggcttc
    35941 tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc ttgttcgaaa agcttttaaa
    36001 cctaactcct tcagtaaacg aatatcctct tcaaatcgat gataaaaatc aatcgcttca
    36061 tgagaaagat aatggagtga ttcgtctaaa gcaatctccc aaatcttttt ttcttcattc
    36121 cactgaatac taggatgctg gttcatgatc ccgataagcg tatctgtcac attaggtaat
    36181 ttgccgtctt ccaaccatgc accttcacat tgattagcag ctactgctcc gccccataaa
    36241 aaattttctg gaaatatttc ctgatccata actatctctc ctcgctatca acaatttctt
    36301 acttgaagta tagctcgatt catcaaaaca agatattcta actatttcgg cagaagagaa
    36361 gaaaaaaatt gaatgctact cttcattttt aatatttata tataacaaat acataagacg
    36421 atccccgaaa gaaaacactc gttaaccatc taagcaaacg atttatttta cttccaacac
    36481 aaatgcttca taaggtcgta aatcaacttt gccgtctggt aactgtttca gaggataatt
    36541 tgacacaata acccttaaag cttcacgtgg aatagtgaac gtctgctttt cactggacat
    36601 attacaaata acgagccatt gctggtcttc atatatgcga gaataggcaa agacatgctc
    36661 aggcgtcttt tccactaatt catagtctcc ccagacgatg atgtcatgtt ttctacgcaa
    36721 atcgatcaat ttcttgtaca tataaaaaac agaatcttga tctgctaacg cttcttctgc
    36781 attgatttct ttataatttg gattgactcg aagccatgga attccagtag taaacccagc
    36841 atgttcacgg gcgttccatt gcataggcgt cctagcattg tctcttcctt tcgccttgat
    36901 tgattccaag atttcctctt ttgtaaatcc agaagaaatc ctttcgttat acatattgat
    36961 tgtctcaata tcttttgctt ccgcgatatc atcgattggg caattcgtca tgcctaactc
    37021 ttctccttga tagatataag gggtgccttt catcatatgg agcaagatag caaacatctt
    37081 ggcactttct tttcgataca catcgtcatt tccccaacga gaaacaattc gcggaagatc
    37141 gtgattattc caaaataaac tattccatcc ttcatcacct aaagatgttt gccatttgga
    37201 taatactttt ttcagttcag agatactcaa gggtttcaaa tcccatttgt ctttgccttc
    37261 ttgctgatct aatcctatgt gttcgaattg gaagaccatg gataattcat ttcttttagg
    37321 attggaataa agtttagcga tttcggtagt tgctccccat gtttcgccaa cagtcatcag
    37381 atttttgtca ccaaaagttg cttgattcat ttcctgaaga tactcatgca gtttaggtcc
    37441 attacttgtg atttctttat ccggttcttt gccaatcata tcgatcacgt ccatacgaaa
    37501 gcccccaata cctttatcga tccagaaatt catcatttga taaatctctt ggcgaacctg
    37561 tttatttttc caattcaaat caggttgacg cttgctatac aagtgcagaa aatattgccc
    37621 cgttttttca tccaactgcc aagcagaacc actgaatatt gagcgcaatc tattaggctc
    37681 ttttccattg accggatcac gccaaatata atagtcccga taaggatttt ctttattttt
    37741 acgggcctca atgaaccacg gatgttcatc agaagtatga ttcaccacca gatccatgat
    37801 gatacggata tttcttttct ttgcttcttc aatcagttct tccatttctt ccatcgtacc
    37861 gaattctgcc atgattgctt gataattact gatatcatac ccattgtcat cattcggaga
    37921 ctcgtatact ggagaaagcc aaattgcatc aatacctaaa tacgccagat aatccaacct
    37981 ggaaataatc ccccgcaaat ctccaatacc atcttgattt gaatcttgaa aacttcttgg
    38041 atagatttga taaacaactg aatggtgcca ccatttttgt tccatgattt tcctcctata
    38101 aatggatgtt tctatttttt gcttaatcat ttattttagt ctctctcact aaaaattgta
    38161 acgctttcaa atagctattg caccttgtta tcggtaacag aataaaacaa aaaaacagct
    38221 gaggaaattc cctcaactgt tttttgaaag gattgattta tgcagggaag aatgctgatt
    38281 tcacaacttc tttataaacg gtgttcactc agctgtccct tggtattagc tcaaaaaacg
    38341 gcaaaatata agaaactatt ttttcgtttt tttctctaat actcaggcca gaacgctgat
    38401 ttcacaacct ctttatttca cattcaatat ccacatgaat cccacaaata caaagaacag
    38461 accatacatg attggagata cttcttttcc tcttttagct gctaacatgg taattggata
    38521 gaagataaaa cctaatgcaa tcccatctga aatactataa gtcaaaggca tgccgatcaa
    38581 gatcaagaat gaagggatcg caatctctaa gtttccccat tcgatttgtc gcaaagattg
    38641 tgccatcaat actcctacca taatcaatgc tggtgcagtt acttgtgaag taacaactga
    38701 tagtaatgga gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat
    38761 acctgaacgt cctccaacgg cgataccagc tgatgattct acaaatgcac caacaggaga
    38821 agtaccaaat aaggaacccg caagcatagc tgttgaatca gaagctaaag ctttccctac
    38881 acgaggcatc ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc
    38941 tgctgtatca aagaaagtca caagcaagaa tgtcagcact acgacccaca tttgaagaga
    39001 attgatgtcc cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc
    39061 ggcaacaatt tcagaaggca ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc
    39121 tgccatcccg atgaagatgc cacctggtac acgccgaaca agcaagatag cagttacgac
    39181 taatccaaaa atcgtcaacc aagtagaacc aacgttgagt gatcctaatc ctactaatgt
    39241 agattcattc gaaacgatga tcccaccttc gctcagacca aggaaagcga taaatagacc
    39301 gataccgcca gaaatcgcat atttcaaatc tgctgggatc gcatcaatga tcatttcccg
    39361 cagcttgaag attgtgatca aaataaaaat caaagaagca acgaatacgc cggctaatgc
    39421 tgtttcccat ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag cattgatccc
    39481 aagagcaggt gccgttccaa ttggataacg ggctaatacc cccattaaaa cacagccgat
    39541 cgcactggct aatgcagtcg cagtaaacac tgctccttca tccattccag aagcacctaa
    39601 tacagttgga tttacaaata agatataagc cattgagata aatgttgtaa atcctgccag
    39661 tacctcacgt tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa gtattttttc
    39721 cttcatcttc catcccccta aatccgaaca ttataaataa agtccgttaa aatttcgatt
    39781 tattaataaa acaacgtgtt ttatcatata ctaaaacaaa acataagtaa acaaaaaatt
    39841 cggattttaa gcaaaatccg aacgttccat ctgattaaat ccctttatct caaagttcat
    39901 gaacattcac tttggattac aagaatttct ttattgtgtt aataaagttt cttcattcta
    39961 gtctcaaaaa tcaatagtgt gatttgattt taccatttcg tttctgttct tctttgatga
    40021 attcttttgt atcgacagga ttcagattat ttagtccgcc aaaacctgta tcgaccattt
    40081 ttccccagcc ttttgtcttc ttttttcctg atttgagatt ctttgctcga agcttctcac
    40141 tatgttttgc tcgccgatac ttcaaataac cttctaatct tcccattaga tccactgttt
    40201 ttcctccttt ttattcaatc agacgactca gcttttccta taacaaaata attgccttca
    40261 gggtcagaaa aattgaaagt caagatgcca ttgttatctg tgatttctcc tggttcttcc
    40321 atgttctcgt agatagtttc aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct
    40381 aatacctctg gtgaaaactt ctgcacaaat acttttggga atacgcctaa ttctaattct
    40441 tttgagatgc tgagtacaat actcttaaat tcttctggca attcgattgt ttcagaaatt
    40501 tctgcaccaa ttttttctac aaagaatcgg caaatcatct ctacgtcatt gacataaagc
    40561 atcacacgtg ttttttgtat catcatttga cccttctttc tatttgattt tctttcctgt
    40621 ctataaactg attccattat atcacgaaag cgcaaacacc ttgtgtttga tactattcaa
    40681 gaaaaataaa gcaatagttt ctaggattgc tttcctccac aaactttttt tacaaaaaaa
    40741 aaacggcaat gaatgccgct ctt
//
LOCUS       DAXWLG010000005        39904 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXWLG010000005 DAXWLG010000000
VERSION     DAXWLG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.005"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE4T_000194"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE4T_000194"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFF8385513.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE4T_000195"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE4T_000195"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFF8385514.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE4T_000196"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE4T_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFF8385515.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE4T_000197"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE4T_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFF8385516.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE4T_000198"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE4T_000198"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFF8385517.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE4T_000199"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE4T_000199"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFF8385518.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACGE4T_000200"
     CDS             complement(8054..8524)
                     /locus_tag="ACGE4T_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFF8385519.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACGE4T_000201"
     CDS             complement(8570..8995)
                     /locus_tag="ACGE4T_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFF8385520.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACGE4T_000202"
     CDS             complement(9062..9331)
                     /locus_tag="ACGE4T_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFF8385521.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACGE4T_000203"
     CDS             complement(9342..10937)
                     /locus_tag="ACGE4T_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8385522.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE4T_000204"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE4T_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFF8385523.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE4T_000205"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE4T_000205"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFF8385524.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACGE4T_000206"
     CDS             15403..16377
                     /locus_tag="ACGE4T_000206"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8385525.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACGE4T_000207"
     CDS             16547..17947
                     /locus_tag="ACGE4T_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFF8385526.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACGE4T_000208"
     CDS             complement(18025..18747)
                     /locus_tag="ACGE4T_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8385527.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACGE4T_000209"
     CDS             complement(18826..19332)
                     /locus_tag="ACGE4T_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8385528.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACGE4T_000210"
     CDS             19455..21254
                     /locus_tag="ACGE4T_000210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFF8385529.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="ACGE4T_000211"
     CDS             complement(21322..22896)
                     /locus_tag="ACGE4T_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFF8385530.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLHQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="ACGE4T_000212"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="ACGE4T_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFF8385531.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="ACGE4T_000213"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="ACGE4T_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFF8385532.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="ACGE4T_000214"
     CDS             complement(24673..25311)
                     /locus_tag="ACGE4T_000214"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFF8385533.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /gene="rpe"
                     /locus_tag="ACGE4T_000215"
     CDS             complement(25312..25962)
                     /gene="rpe"
                     /locus_tag="ACGE4T_000215"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8385534.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="ACGE4T_000216"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="ACGE4T_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFF8385535.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="ACGE4T_000217"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="ACGE4T_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFF8385536.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="ACGE4T_000218"
     CDS             complement(29105..29845)
                     /locus_tag="ACGE4T_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFF8385537.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="ACGE4T_000219"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="ACGE4T_000219"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFF8385538.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /gene="fmt"
                     /locus_tag="ACGE4T_000220"
     CDS             complement(31216..32154)
                     /gene="fmt"
                     /locus_tag="ACGE4T_000220"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFF8385539.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="ACGE4T_000221"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="ACGE4T_000221"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8385540.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="ACGE4T_000222"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="ACGE4T_000222"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFF8385541.1"
                     /translation="MILTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /gene="rpoZ"
                     /locus_tag="ACGE4T_000223"
     CDS             complement(35314..35619)
                     /gene="rpoZ"
                     /locus_tag="ACGE4T_000223"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8385542.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="ACGE4T_000224"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="ACGE4T_000224"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8385543.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36725..37030)
                     /locus_tag="ACGE4T_000225"
     CDS             complement(36725..37030)
                     /locus_tag="ACGE4T_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385544.1"
                     /translation="MTKEVEIMKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACAD
                     YNENELFILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37027..37917)
                     /locus_tag="ACGE4T_000226"
     CDS             complement(37027..37917)
                     /locus_tag="ACGE4T_000226"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFF8385545.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37937..38155)
                     /locus_tag="ACGE4T_000227"
     CDS             complement(37937..38155)
                     /locus_tag="ACGE4T_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFF8385546.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38125..39825)
                     /locus_tag="ACGE4T_000228"
     CDS             complement(38125..39825)
                     /locus_tag="ACGE4T_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFF8385547.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
BASE COUNT        10776 a         8697 c         7256 g        13175 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgatgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggtaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cctagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttagtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ccaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatatca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgacatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac cgagaaaatg gtttttgaac atcacttatt
    36601 caaaataaaa aaagattgtg acacgaattt tgtcacaacc tttaaatgta ttcctagagc
    36661 aaagaagcta agcgaagaaa tccaattctg attatttatt gtatttcttt gttcagcatc
    36721 cttgctaaaa gcccatattg gacaaagtat tcactacacc catgatctca tcttttcgag
    36781 aacgtgcttt ttctgtcgga tcttctacct cttctgtgtc aattcgaaca ataaatttcc
    36841 gattcatcgt cacgtaagca ccgtcgttga tcaaaataaa cagttcgttc tcattatagt
    36901 ctgcacaggc atctttccat acgtcaaagc cgtttttttc agaagggata tcccgtttaa
    36961 ccacagtttt tccacttaat tcgtggaacg ttacgtcata atatttcatt atctctacct
    37021 ctttcgtcat tcaatatttt gtacttgttc tctgattttt tctaagatcg tcttagactg
    37081 gacgacttga tttttaattt cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg
    37141 attcatttct tggatcagga aatctaattc ccgacctaca ggacattgtg cttcgataag
    37201 ttcatctagt ttttgtacat ggatcgccag acgatccagt tcctcgtgaa tatctccgcg
    37261 ttccaacaag atggctagct ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa
    37321 ttcctccagc tttttttgat accttatttg aaattccttt tcatagattt ctacaaaact
    37381 atcgagtttt tttagtactt ctttgaattc ttcagtattc tttttcaaca ctaaagctaa
    37441 agcttgacct tctttttctc gactcgcctc aatagaatca agtgcgcttt tcaatgtatc
    37501 tagagctaac tgtgccaatt cttccatgct ctcttccggt ttttcttcta ccgttacgaa
    37561 gttttcgtcc agcgcgaagc gttccgccat ccgactaatt gacacttgat tttgactacc
    37621 gtatcgttgt tgaattccgt ctgtcaattg agtgataagt gaatcaatca attcccagtt
    37681 gatgacgatc tgtttttcgt ttttgccaat ataagctaga ttcacaaata cttcgattcg
    37741 accgcggctt aactgctgtt taataagctg acgaaaatcg ttttcaacaa agttcaggat
    37801 tttaggactt cgtatttgaa catctagaaa acgctggttg acacttttta tttcaatatc
    37861 taactgatac gcttttgttt cgcggatggc tttgccaaaa ccagtcatac ttttcattcg
    37921 acagggtcct tcctcattac atattttcta ttgctttttg cagttgttca aactcttcgt
    37981 tcgtcaatgt caggcctttc cccatttttt catggtctgg cgcccaatct cgaagatcga
    38041 attttggcgg gcgaccattc cagctgacta ggttcagttc tttacgccac cccttgttgt
    38101 tttctgacaa tatagcgatt tcttctacga tttcatatga aaattcttgt gccatggttg
    38161 ttcctccttg agataatata aatataggat cagtaatgtg tcaactggtt gcttaagcaa
    38221 ttcatgctgc aaacgatcct tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt
    38281 cgtgcgctca ttttgtattt tttgctggta ttttcgatac cttcgccatt cttcagtact
    38341 taattgacca atttgtgcca ataaatcatc cggcatactt tttatatctt gttggcagat
    38401 catttgaatc atcatgtcta cttgatagat ttcattgggc agtaaaaaat gtttttttgc
    38461 taaaagttct agcatcgcca atccgcatgt ctctggtacg accctgttct tatataaatt
    38521 acgcagtttt tgccaaaatt ttatgctttt gaaaaaagat aattctaact gaatttcttt
    38581 tgttaagtaa gacaaaaatc ctcgccagta tgttcttgca ggtttaacag aatgaaatgt
    38641 ataccagttc ccatagtaat agcctaatcg ttttttcatt tccagatagc ctaattgtaa
    38701 atttaattgg ttcctttggc tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg
    38761 acatttccaa tggataaaaa tgtctggcat acgaattttt ttcgctgggc ctggcccttg
    38821 cacatctacg acaaatgcct ctgttgctcc tttgttaatc gcaatatcaa taggtatttt
    38881 gttgcgatac ccaccgtctg catatttatt ttttccgatc tttcgataag ccatagcggg
    38941 ataaaatgaa gcagaggcga gtatccaatc tggtatttct tctgtttttg tctgttggat
    39001 atcagtcact acttccgcta gttctggcag tttagtagac accgtatata aacgtatttt
    39061 gggattatgt ttgagtaagg acaaatccaa attgtctttg accatctttt ccaatggagc
    39121 agaagaaacc ccgccttcaa ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa
    39181 gcgttcacga aggtcttcaa ctgcagccat ttctggtaac gcaagcactt gtcttgtcga
    39241 cagctttttc cataaattca cagctttttt catatctcct tgtaaaatca atgcgccgtt
    39301 caacgcacct accgaggtac ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc
    39361 ctgccacaca ccaatctgat aagcaccgtg tgctcctcct ccacctaaaa caagtgctgt
    39421 atgatagacc agctttttat ttaattgtgt ggttccttga tagccgtttt tatgaaaaat
    39481 acgcaaccat tcttctgata gaggagattg aaataaaaaa caacagtttt ctttaataaa
    39541 aaaacgagcg caagcttcca tataccgaaa aaattttttc cattcaaaag tttcaccttc
    39601 aatcaacaga ttattaaaaa taacatctcc tctactttta gcaatagtaa aatagacggt
    39661 atattttttc gatactgctt taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc
    39721 tctactttct tgatgacttt gaaaaaatgg aaggggttgt tttgcttgct ggataggcga
    39781 atacagcgac attttctgtg aaaagtaaac tttttttatc tccatcgcgc tctccttttt
    39841 cttcaagtat accagtacgt cataaaaagg tcatgagtct cgtattcgac tttgtagatt
    39901 ccat
//
LOCUS       DAXWLG010000006        38842 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXWLG010000006 DAXWLG010000000
VERSION     DAXWLG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.006"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(2..2383)
                     /locus_tag="ACGE4T_000229"
     CDS             complement(2..2383)
                     /locus_tag="ACGE4T_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8385548.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITERTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2373..3020)
                     /gene="recU"
                     /locus_tag="ACGE4T_000230"
     CDS             complement(2373..3020)
                     /gene="recU"
                     /locus_tag="ACGE4T_000230"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFF8385549.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3084..3623
                     /locus_tag="ACGE4T_000231"
     CDS             3084..3623
                     /locus_tag="ACGE4T_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFF8385550.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3713..4132
                     /gene="gpsB"
                     /locus_tag="ACGE4T_000232"
     CDS             3713..4132
                     /gene="gpsB"
                     /locus_tag="ACGE4T_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFF8385551.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4154..4521
                     /gene="rnpB"
                     /locus_tag="ACGE4T_000233"
     ncRNA           4154..4521
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGE4T_000233"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4730..5896
                     /locus_tag="ACGE4T_000234"
     CDS             4730..5896
                     /locus_tag="ACGE4T_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFF8385552.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5942..7438
                     /locus_tag="ACGE4T_000235"
     CDS             5942..7438
                     /locus_tag="ACGE4T_000235"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFF8385553.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7519..8202
                     /locus_tag="ACGE4T_000236"
     CDS             7519..8202
                     /locus_tag="ACGE4T_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8385554.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8847..10037
                     /locus_tag="ACGE4T_000237"
     CDS             8847..10037
                     /locus_tag="ACGE4T_000237"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFF8385555.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10139..11092
                     /locus_tag="ACGE4T_000238"
     CDS             10139..11092
                     /locus_tag="ACGE4T_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFF8385556.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11161..12510
                     /locus_tag="ACGE4T_000239"
     CDS             11161..12510
                     /locus_tag="ACGE4T_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8385557.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12693..13016)
                     /locus_tag="ACGE4T_000240"
     CDS             complement(12693..13016)
                     /locus_tag="ACGE4T_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385558.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13145..14308
                     /locus_tag="ACGE4T_000241"
     CDS             13145..14308
                     /locus_tag="ACGE4T_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8385559.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASRQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14378..15727
                     /locus_tag="ACGE4T_000242"
     CDS             14378..15727
                     /locus_tag="ACGE4T_000242"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8385560.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15772..16203)
                     /locus_tag="ACGE4T_000243"
     CDS             complement(15772..16203)
                     /locus_tag="ACGE4T_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8385561.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16591..19233
                     /gene="alaS"
                     /locus_tag="ACGE4T_000244"
     CDS             16591..19233
                     /gene="alaS"
                     /locus_tag="ACGE4T_000244"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFF8385562.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19433..20029
                     /locus_tag="ACGE4T_000245"
     CDS             19433..20029
                     /locus_tag="ACGE4T_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8385563.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20151..21299
                     /locus_tag="ACGE4T_000246"
     CDS             20151..21299
                     /locus_tag="ACGE4T_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8385564.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21337..21750)
                     /locus_tag="ACGE4T_000247"
     CDS             complement(21337..21750)
                     /locus_tag="ACGE4T_000247"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFF8385565.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21829..22260
                     /locus_tag="ACGE4T_000248"
     CDS             21829..22260
                     /locus_tag="ACGE4T_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFF8385566.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22359..22592)
                     /locus_tag="ACGE4T_000249"
     CDS             complement(22359..22592)
                     /locus_tag="ACGE4T_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8385567.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22775..24445
                     /locus_tag="ACGE4T_000250"
     CDS             22775..24445
                     /locus_tag="ACGE4T_000250"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFF8385568.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24592..24978
                     /locus_tag="ACGE4T_000251"
     CDS             24592..24978
                     /locus_tag="ACGE4T_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385569.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24965..25663
                     /locus_tag="ACGE4T_000252"
     CDS             24965..25663
                     /locus_tag="ACGE4T_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFF8385570.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25668..26132
                     /gene="lspA"
                     /locus_tag="ACGE4T_000253"
     CDS             25668..26132
                     /gene="lspA"
                     /locus_tag="ACGE4T_000253"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFF8385571.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26129..27040
                     /locus_tag="ACGE4T_000254"
     CDS             26129..27040
                     /locus_tag="ACGE4T_000254"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8385572.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27275..27814
                     /gene="pyrR"
                     /locus_tag="ACGE4T_000255"
     CDS             27275..27814
                     /gene="pyrR"
                     /locus_tag="ACGE4T_000255"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFF8385573.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27828..29111
                     /locus_tag="ACGE4T_000256"
     CDS             27828..29111
                     /locus_tag="ACGE4T_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFF8385574.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29124..30050
                     /locus_tag="ACGE4T_000257"
     CDS             29124..30050
                     /locus_tag="ACGE4T_000257"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFF8385575.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30069..31349
                     /locus_tag="ACGE4T_000258"
     CDS             30069..31349
                     /locus_tag="ACGE4T_000258"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFF8385576.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31354..32430
                     /locus_tag="ACGE4T_000259"
     CDS             31354..32430
                     /locus_tag="ACGE4T_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFF8385577.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32435..35620
                     /gene="carB"
                     /locus_tag="ACGE4T_000260"
     CDS             32435..35620
                     /gene="carB"
                     /locus_tag="ACGE4T_000260"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFF8385578.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35674..36450
                     /locus_tag="ACGE4T_000261"
     CDS             35674..36450
                     /locus_tag="ACGE4T_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFF8385579.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36447..37379
                     /locus_tag="ACGE4T_000262"
     CDS             36447..37379
                     /locus_tag="ACGE4T_000262"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFF8385580.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37376..38092
                     /gene="pyrF"
                     /locus_tag="ACGE4T_000263"
     CDS             37376..38092
                     /gene="pyrF"
                     /locus_tag="ACGE4T_000263"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFF8385581.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38099..38728
                     /gene="pyrE"
                     /locus_tag="ACGE4T_000264"
     CDS             38099..38728
                     /gene="pyrE"
                     /locus_tag="ACGE4T_000264"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFF8385582.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
BASE COUNT        12498 a         6887 c         8478 g        10979 t
ORIGIN      
        1 actatctgtt tccagaagct gaggtgctgc tggatggtgt atggtcattt gcagcatccg
       61 ctccgctagt tgctggctca ctggaagaag caggaggagc agatgaagac ggtgtcgagc
      121 tttcttcgga tgaagaagaa ggaaccgttg ttgaagacgc tggctcgctt gtttctgctg
      181 tcgtgctttc ttttgaggac tcagcagttg attcgctttg actagtagag ctagagcttt
      241 gagtcgttgt ctctgttgtt gagcttccag gagtctggac atagctggaa gatggaatag
      301 ttgttgatgg tgtaattgca ttcgatctag ctgtatattg atctttgtag tacagttcac
      361 cgcccacacg aattaatccg cttggcatct cccagtctgt atttgtcacg ttagctgaaa
      421 catactgcat aagctcgcga taaacatcag aggctacgtg tgacgattct gaagtgacag
      481 gtgtcatttt tttattgtaa cctgtccaca cggaaatcga ataatttggc gtatagcccg
      541 caaataggat atctggatac accccagaag aaataccaag ttttgcatac tcgtcatctg
      601 tgtaattgga tgttccggtc ttaccggctt gatataaccc agcgatttgc gcattcgtac
      661 cagttctttc agtgatcgta tcttttaaaa tgtctgtgat catataagct gtttctggag
      721 acatggctgt ttttccatct ggctcataag tttcttctgt tccgtcttgg aagacgattt
      781 tattaacata ttgaggtttg taataagtcc ctccattggc gaaagccgca taggctgctg
      841 ccatttttaa ggaagaagct ccgtattttg tcccatcttg ttcttccgta ttactagaga
      901 tcgcattcga ttgatggatc gtactgtatt cgatcccaag attttttaag aaagaagcca
      961 ctttatctga accgacttca ttgaataatt tgactgctgg aacattacgg gataagtaaa
     1021 gtgcttggcg taaagtaatg gtgcccatat attgattatc ccagtttcct acaggagtcg
     1081 aagtcccttc atagttatac ggtgcgtcag taatagtttt tccagtagaa tatttcaaat
     1141 attcaaaagc tggtccgtaa tctgtgacag gtttcatcgt agaaccaaaa tcacgggaag
     1201 tattcactgc taagttgttg cctaaagtta cgtcttctgc gatgtgacgt ccgccaatct
     1261 gggcttttac tttcccggta ttggtgtcga taagggtgga ggcaacttgc atctcgtcgt
     1321 ctgggtaaga gacatactga tccgtgttga cgatatcata tagtttcttt tgggcgtcca
     1381 gatccaaatt cgtataaatc tctaaaccgt ctgtgtagac attcttatct gttttttctt
     1441 gtacttcatt gatgacttct ttgacataat tatcgacgat cttagtattg tcatcggatt
     1501 gagttagttc ttgcagacca tccgttaccg gtacattaac agcttgatca tattctgttt
     1561 gagagatttt ttcgttttga agcatcgtat acaaaacagt atctcgtctt ttcttcgcct
     1621 gatctggata aacataagga tcataggcgg aaggggcttg aggcatccca gcaagcaatg
     1681 ctgtttgcgg taaagaaagt tctgataatt tcttaccaaa gtacatctca gaagctgtct
     1741 ccatcccgta caagccgttt gacatataca ctttatttac gtagtaggtc aagatctctt
     1801 gtttcgattt tttttgttcc aatcttacag ccatccacgc ttcttgggcc ttgcgtttca
     1861 gagtctgatc ctcggcactg gtggagaaga aggaaagttt gatcaattgc tgtgtcaaag
     1921 tacttccgcc ttgcagtccg ccagaggtaa agttagagag ggcagaaccg atgatccgaa
     1981 tagggtcaac cccgatgtgt ttataaaaac ggcgatcttc taccgacacg atcgcatctt
     2041 ctaatgtttt cggtaattca ttcgcagaaa ttttttcacg cttttctgcg cctaaatctt
     2101 cgaatagttc gccatcttgt gtatatagtt tggaagatac ggtagcgtct aattttttat
     2161 ccgtcaattc gggcgcatct tttgcgtaat accagaaaag tcctacacct gctaaaaaga
     2221 ggatacagaa aaatgagagt attcctaaaa ggatcttgat aaataatcct tttttatgtg
     2281 ttccagagga ctttccagaa cctttgccag aattcttctt cacactcttt ttaggagtag
     2341 gctgtttctg tcgtctagag cttctagttt gttcatttgc cattcttgct ttctccttca
     2401 atagataatg ttgataaata gaagtcaacc gcgtccaaat aaggaatccg cggagaaata
     2461 ccagttttga tctctattcc tagttcttcg atcaaagata aaggtagtga tttgcgtcct
     2521 ttttcctttt gggcgtccca gtaagcaatc aaatgaacac tattcaaata aaaacagcgc
     2581 ttcaaagacg aaaaccataa caagacaaag cagaccccat tttgttcgag gcatgctttc
     2641 atatgttcga tttgatgggc atggaagttt ttaaagggaa aagatgtttt gttcttggtt
     2701 tcttttgctt caaagtccaa gtaaaagcct cgatagacgc cattatagtc tgtagttgag
     2761 gcttgggtaa agtaggcttc cttgatcaca gcagcacttc tcttaggata gtctactttc
     2821 acgatttgga taggggtagg ctttttatgg ataaccgcca acccgcggga aagataatat
     2881 ttattacttt cattgattgc ttcttcaaag cgcatccccc gattgccgaa ttcagtgatc
     2941 tgatttcttt tttcttgaat tttttcgacg ggagaggcat tcttataata aggttggcca
     3001 tttggatagc gcaataccat ctttaatcac actcctaggc cacattatag caaaaaagcg
     3061 gatagaaaga aaaggaatct gagatggata agtcaaaagt aatctatgtg tctgggtatc
     3121 gaagtttcga actcggtgtt tttaaagaaa atgatcctaa aatccaagtt gttaaaaatg
     3181 ttttaaaaaa agaactccat cagttagcag aagaagggct ggaatgggta cttgtcagtg
     3241 ggaatttggg cgtcgaacta tgggctgtgc aaatagtagc ggaattgaaa gaagaatatc
     3301 cggaactatc tcttggtatt ttgtatcctt tttctgattt tggtaataac tggaatgaag
     3361 ggaaccaaga aaaaaaacaa ttagcagaac aattagcaga ttatgtcgaa gctgttagcc
     3421 atcaacccta ccaatcacct agtcagctaa agaatcacac tcgttttata cttgagcata
     3481 cagtgggctg tctgctgatc tatgatgaag aattccctgg aaaaacgaag tatttttttg
     3541 aagatgcaca gaattaccaa gcagatcatc cctacgaaat tcggctgatc agtatggacg
     3601 atcttcaaaa ttttagtgaa taatcgatat tgtgtttgat ttatcaactt ttttcctata
     3661 caatgaaaaa ggtagaaatg ttattacgta aatgaaaact agaggtgtaa atatggcgaa
     3721 tttggtttat agtcctaaag acattttaca acaagaattc aaaacaaaaa tgcgcggcta
     3781 tgatcccgtt gaagttgatg aattcttaga caatatcatc aaagattatg aaacttacag
     3841 caaagaactc cttgctttac aagaagaaaa cgatcgattg agtgcaaaag tagctcaatt
     3901 atctaaaact caaggagcag ctcaaacacg cgtgcaacaa acagaggcac caaagagcgc
     3961 agcagtgacc aattttgata tcttaaaacg cttgtcaaat cttgaacgtg aagtatttgg
     4021 taaaaagctt gatcaacaag caagtgcagt caaaccagca caacctaatc caaataacta
     4081 cacaaatgca gacacaagct tagatgataa tgaaaaaaca cgtcaatttt aatttttgat
     4141 taatgaattt gcggttttcg ggtaatcgcg gtctgacttg atcaggctga ggaaagtcca
     4201 tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata agctaaggta
     4261 ctctttggag taacggcagg aaaacaagct aaggcttcgg ctatgcttaa gtatccttga
     4321 aagtgccaca gtgacgaagc ggctggggaa acccatccgg tggaacgcgg taaacccctc
     4381 gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa caggacaaaa
     4441 aggcagtata actgcagata gatggttacc gctttgtttt ttctccctgg aaaagcaaag
     4501 tacagaacat ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg acaaaagtaa
     4561 tgcgttataa gaaacaagaa aaacatttga aagctatttt tcaatacttc agatgaatcg
     4621 ttcttattac aaaacaaact tttgtatcac cggttattcg gaatttgagt aaaagtgtgg
     4681 ggtaggactg atgtcccacc tccttttttt ataaataagg agattgtgta tggaaaaaaa
     4741 caggaagttc aacctcgtcg caactgcggc aagcgggcta gaagcactgg ttggtaaaga
     4801 attaagagat cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt ttgaaggaac
     4861 aatggaaacg atcgctacag ctaatttatg gttacgtaca gcagatcgag taaaaatcgt
     4921 ggttggggaa ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa aagcattgcc
     4981 ttgggaagat tttcttccac tagatgcagc ttttcctgta gctggaaaat cgatcaagtc
     5041 gaaactatat agcacacctg attgccaagc gatcacaaaa aaagcaatcg ttgaacgtct
     5101 ccgtacctat tatcaccgtc cagcttctgt accactgacg gaaactggcg cacattttca
     5161 gttggaagta gctttgctga aagaccatgt gatggtaact ctggatacga caggaccaag
     5221 tttattcaaa cgtggttatc gcttagaaaa aggtggagca ccattgaaag aaaacatggc
     5281 agcagcactt attatgttga caaattggcg aaaagaccgt ccattttatg atcctgtatg
     5341 tggttcaggt acgctttgta ttgaagcagc cttaataggc cataatatcg caccaggatt
     5401 caatcgtgag tttgcttgcg aatcttggga ttggttcagt caagaaatca tggaaaatgt
     5461 tcgatcagca gccgaagaaa aagcagatta tgatattgaa ttagatatta ctggttcaga
     5521 tatcaatgga cggatgattg aaattgccaa agccaatgca gaagaaatcg gattaggctc
     5581 ttcgattacc tttaagcaac aagcagtcaa agacttcaaa acggataaag aatacggggt
     5641 catcgtggct aatccgcctt acggagaacg tctaggagaa gaagaaactg ttcggaaatt
     5701 atataaagaa atgggcgatg tattccgtcc attaaaaaca tggagtaaat atatcttgac
     5761 tagcgactta gcgtttgagg aattttacgg acaaaaagca accaaaaaaa gaaaattgta
     5821 caatggtgca ttgcgtacgg atctattcca gtattgggga actcgtccgc ctagaaaaaa
     5881 agagaaagac gaataatctt actaattgat tcattcaatt ggatgtttgg aggaataggg
     5941 aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc aatcttctac aacaagcttt
     6001 aggaatcctc gattgggata cacaaacagg tatgcctgaa aaagctagtg cataccgaag
     6061 tgaagtagac agctatcttt acagcattta tttttcaaaa aaaatcggtc caaaaatcaa
     6121 agaagccatt cgctattttt ctgaacatcc ggaagaattg tcggaagttg gaactgccgt
     6181 ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc gtaccggaag atcttatgag
     6241 aaaatataca gctgccgttt cttcagcaca cggacaatgg caaaaagcta gagaaacaca
     6301 acaattttct gattttcaac acgcattgaa tcaaaatatt gaattaacaa aacagttgat
     6361 accttactgg agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc aatacgaacc
     6421 cgggatgact gtcgaaatcc ttgataaggt atttgatcag cttaaagaag gaattctttc
     6481 gatccgccgt acgctccaag aaaaagggac tgaacctgaa acagactttt taaatcggag
     6541 aatgacgaaa aaacaacaga agagatttgt agtgaaagta atcgaacaac ttggttatga
     6601 ttttagtcgt ggtcgtttag atgacactgt ccacccattt atgactggaa ttaatccgaa
     6661 tgatgcacga atcacgacac gatggaacga aacagatttc aaaatggctg tttttggagt
     6721 gatccacgag gctggtcatg gtatgtatga acaagacatc gatgataaat atgcgtatac
     6781 accggtttat gaaggtacat caatgggtat ccatgaatca caatcgttgt ttaacgagat
     6841 cattattgga agtagtcgca gcttctggaa aaagcaatat cctttctttc aagaatgtgc
     6901 agaaggaaca ttcgatgata ttccttttga caaattttat cgttcattga accatacaaa
     6961 atcaagtttg gttcgtatcg aggcagacag tttgacttac cctctccata tcatcattcg
     7021 ttatgaaatc gaaaaaatga ttttcaatga aggggtagat gttgctgaac tacctgaaat
     7081 atggaatcaa aaatatgaag agtatctggg aatacgacca acaaatgatg tggaaggcat
     7141 cctacaagac gttcattggt caggtgcaag ctttggttat tttccctctt atgcattagg
     7201 gtacatgtat gcggcacaat tacttcattc tttgcagcaa gaaatagatg tggaaaatgt
     7261 tctagcgtca gacgattatt ctccaatcaa aaactggatg tcagagcata tccatcaata
     7321 tggtgcatct agaaaaccaa atcaattaat catggatgca acacatgaac cgcttaatcc
     7381 gcaatacctg atcgactata tgaaagacct gtattttaat gtatatcagg taaaataaaa
     7441 tacaatttac aaagatagct gaaattcatc taatctatag atgttttcag ctatttactt
     7501 aagaaagaag tggaacgaat ggataaatta caagtgatcg ctgattatag tatggaccaa
     7561 ttagcgcatg atcaaacagg acatgggagc gatcatacca aaagagttgt caagttagct
     7621 gaaaaaattt tggagacgga accgcaggca gaccgctttg tgacgcttgc agcagcttac
     7681 ctacatgata cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa gcaacagctt
     7741 cgtgtattcc ttcgctcttt gccaatagta gaggaacaga tctcgatgat tttttccatt
     7801 atcgaaaata tgtccttttc taaaaatctc tctgaagcgg ccgaactatc tctggaaggt
     7861 aagatcgttc aagacgctga tcgtatcgaa gcattaggag caatcggcat tttgcgtact
     7921 gcttatttcg gcggtggaca tgggcacccg atttttgatc ctgaactgta tccgcaaaca
     7981 ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga tcaatcattt ttatgaaaaa
     8041 ctgttcttgc tgtctgataa aatgaatact gattacggat atgaagaagc caaacgaaga
     8101 gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg aatggtctat tgacgatgaa
     8161 aattttactc cagacaattg gatcaaaaat aacaaaaaat gatggaggtt gtgacagaag
     8221 tggtcagctc caagatattt cggatcattt tccgtaaaag ttacttctgg aacactgttt
     8281 accaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaata
     8341 gcttctcgtg tttgatgaat attgtaactt gtgattcgga attggcagga gaacaccgtt
     8401 tattcggaag aataggactg tgacaagact tttgtcacag tccttttttc gaattcaaca
     8461 agtaaaactt ttaagaaata ttcagagagt aaaattaaaa attgctgata aacgaagatg
     8521 gaaattttat gtatttttat tggtctatgc aataaataaa ttaaatattc agaatacaat
     8581 tgattttaac gaaagggctt ctatcaatgg ttgtgttgta tttatctgcc caggtagagt
     8641 aaaatagatc attatctaaa aggtaattgt tcatactata taagtcattt gaaagtaaaa
     8701 agatactgta aataaaaaat gtggttaaat aattctcata atcagtgtaa gcgttatcaa
     8761 aaatatagca tacttgcttt gtagatgaat gacacgatat atgtttggta tgttctgcaa
     8821 gacagatgaa tatggaggtg gagacaatgt cttccaaaga actaacaatg tatttagaga
     8881 aagaattgga tcaactgaaa gaaaaggggt tatacaatac gatcgacata ctagaaagtg
     8941 aaaatggtgc ttgtatcatc gtagacggaa aaaagatgat caatctagca tccaacaatt
     9001 atcttggatt tgccaatcgc gaagaattga aaaaagcatg tattgaagca accgaaactt
     9061 atggtgtagg agcaggagca gtccgaacga tcaatggttc gctgaaaatc catcagcaat
     9121 tagaagaaaa aatcgcagaa ttcaaaggga cagaagcagc aatcgccttt caatctggat
     9181 tcaactgcaa tatgggcgct atctctgcag taatgacaaa agaagatgct atcttatcgg
     9241 atgaattaaa ccatgcatct attattgacg gttgccgctt atccggagca aaaatcatcc
     9301 gaattaaaca tcaagatatg aaagaccttg agaaaaaagc aaaggaagcg atagaaagta
     9361 agaaatataa aaaaatcatg tatatcacag atggtgtttt ctccatggat ggagacattg
     9421 cacgattgcc ggaaattatt ccgattgttg aaaaatatgg actgatcacc tacgtcgatg
     9481 atgcgcacgg ctcaggcgta actggaaaag gtgcaggaac cgtcaagcat tttggattaa
     9541 gcgacaaaat cgatatgcaa atgggcacgt tatcgaaagc tataggtgtt gttgggggtt
     9601 atgtagctgg atcgaaaaca ctaatcgact ggttgaaagc tagaagtcgg ccattcctat
     9661 tttctacttc gctaactcca ggtgcagctg cagctgcatt ggcttcgatt actttgatgc
     9721 aagaacatcc ggaacttgtc gaaaaagttt gggaaaatgc taattatttt aaagaagaat
     9781 tgaaaaaagt aggctataac atcggtatgt ctgaaacacc tattactcca gtcattttgg
     9841 gagacgaaaa agtgactcag actttctcta agaagctgat cgaacatggg atttatgcta
     9901 aaccaatcgt ttatccaact gtgcctcttg ggacaggcag gatcagaaat atgccgactg
     9961 cggaacatac gagggaaatg cttgatgaag cagtagcagt ttatcaaaaa ataggaaaag
    10021 aaatggaaat tatttaacta aatttagttt tttcgtctat ttaaaattag agaacatgtt
    10081 cacgcatata ttgttaatca tctgaagata gtagtaaaaa agatcaggga ggatcatcat
    10141 gaaaaaagta ttggtgacag gatgtttagg acaaattggt tcagagttga tcacacggct
    10201 acgtcaagaa ctaggcagtg aaaatgtcat tgcgacagat atacgcatgc ctgaagtcat
    10261 tgagaaaaac gatatctttg agatcctaga tgtaatggat tacgaaaaaa tgcgcagcat
    10321 agcagaacaa tatcaagtgg acacattgat tcatttggct gctcttttgt cagctgttgc
    10381 agaagcaaaa ccacaattag cctggaattt gaacgtgact gggctggtca atgcattgga
    10441 agtcgctaga gaattccagc tgaaattttt tacgccaagt tcaatcggcg cgtttggtcc
    10501 aaatactcca aaagatcata ccccacaaga tactcttcag cgtcctacaa ccatgtatgg
    10561 ggtaacgaag gtagctggag aactactatg tgattattat tatgtgaaat atggcgtaga
    10621 tactcgtggt gtccgtttcc ctggattgat ttcttatgca accttgccag gaggcggtac
    10681 gacagactac gcagtcgata tttattattc agcaatcaaa gaaggaaagt acactagttt
    10741 cattgatcaa ggtacatcaa tggatatgat gtatatgcca gatgcgatcg atgccattat
    10801 aaaactcatg aatgcagatc ctgatcgatt ggttcatcga aatgccttca atatatcggc
    10861 gatgtctttt gacccggaag agatcaaagc atctattcaa aaaatcatac cagattttga
    10921 aatggattat gatgtggatc ctgtacgtca gtcaatagca gaatcttggc caaattcact
    10981 agatgtttct tgtgcgcaag aggaatgggg cttttcgccg aaatacgact tagatgtgat
    11041 gacaaaggat atgttggaaa aactgaaaga aaaattggta gagacggtat aaaacaagaa
    11101 ccacatccaa aaagtttttt atacttttgg gattggctat ggtatgaaag gggacaaggg
    11161 atggaagaat atcaagccac gccagtaaaa gaagtaaaaa tagaaaatga attaaaacgt
    11221 acgatgggat tttttactgc tttatcgact gttatgggca cagtgatcgg tgcaggggta
    11281 ttttttaaag ccgcaagcgt agcagaagtt acaggatccg ccagtttgca tatgttttct
    11341 tggtttctcg gtggtatgat ttctgtctgt gcaggactaa caggagctga actggcagct
    11401 gctattccag aaacaggagg gatgattaaa tatatcgaac ggatttacgg aaatacagca
    11461 gcttttttat tgggctgggc gcaagtcgtg atttattttc cagcaaatgt agcagccttg
    11521 tcgatcattt tcggtacaca gttcgtcaat ctatttggtc tttcccagtc aatgattgtt
    11581 cccgtagctg ttacagctgc tgtatcgatc ctgttgatca attttttagg ttctaaagct
    11641 ggaggggctt ttcagtcgat tacgcttgtc tgcaagctta ttcctttgtt tgtgatcgtc
    11701 attttcggtt tgttccgaca agaaggagta gacttccaac tgtttccgat ccaagcagga
    11761 gaaaatcttt catttttctc agccttggga gcgggcttgc tagcaacgat gttcgcatac
    11821 gacggttgga tacacgtagg aaatatttcg ggagaattga aaaagccggc gaaagatttg
    11881 ccaaaagcaa tttctttagg aatcatcgga atcatgatcg tttatttatt agtaaatgcg
    11941 gtgttcttaa gaaccgcatc tatcgacgga gtcgcaggaa acagcaatgc ggcaagtgac
    12001 gttgcaaaaa tgatttttgg cggcttcggt ggaagactgg tcacagttgg tatcttgatt
    12061 tctgtttatg gaaccatcaa tggctatacc cttactggaa tgcgtctccc ttatgttatg
    12121 gcaaaagaaa acaacctgcc ttttagcaag ctctttgcta aacttcatga taaaactaaa
    12181 gttcctgtag ccgcaggtat cttagagcta gtcatcgcta ttggcatgat gatgatcggt
    12241 ggttttgata cactaacgga tatgctgatc tttgtcattt ggatttttta tacgatggtc
    12301 tttgttggtg tcattcttct tcgtaaaaaa gaacctgatt tgtttcgacc atacaaagtt
    12361 ccaatgtatc cgtttattcc actagtagca atcattggag ggacttttat cgtatcaagc
    12421 acgcttatca ctcagacctt tttagcttcg atggggatag tattgacact tgctggtatc
    12481 ccgatttatc tctatttaaa acgtagatag ctaaaacatt cgaattttat tagaactgca
    12541 cttctctgcc aaacaaaata ttttatttaa atgaggagta cgtggaagaa atgtcaaaag
    12601 tactcgttaa ttttgatcac gtaaatgtcg ttttgtctac gatgaaatag caaagtaaaa
    12661 gacagaaatc gggctcaacc gatttctgtc ttttactttg atttgcgtaa ctgaactatt
    12721 cggcgtacgc cgcgataaat atttaataaa accaagcaac cagaaatgaa aatgggaagc
    12781 caaatcagat agacagggta gccaagcact ttagacaaag catgtggagt cattttatcc
    12841 atttcaagaa gtgcttgctg ccaagtctga agattgaaga ctagcatgaa acccgaaata
    12901 atcaggagaa taaatcctaa ataatgaatt gtagttcttt ttcttacata agcgatcgta
    12961 gcataaagga acagtaaaaa aataggtacg ccaaaaacaa agtacatatt cagcatctcg
    13021 acaccccctt tgtgtttatc ttactttact ttgaaagaaa tgagaaataa tagcctcgtt
    13081 acttgcttga aacagcaaag gactgctaag ataagagaga tacaaagaat caaggaggag
    13141 tcatatggca agttatatcg aaccgattcg ttttgcgctg attgtttttc catttttagc
    13201 cttagcgatc tcatctgttt tttttattta tgaatatcga aagtatggaa catttctagt
    13261 aacaagggca gttattttgt attctttcgt tttttattta ttgtgtgcgt attttttggt
    13321 gatcttacca cttccggcaa aggagacggt tgcccaaatg acagggccaa agatggagct
    13381 gcatttattt gcttcaaggc aacatttcag agacaacacg gtattggtct tgtctgaacc
    13441 tagtacctac ctgccggcaa tgaagcagag tgtatttttg gaaccagctt ttaatgtttt
    13501 attactcata ccatttggca tttatttaag atattatttc cggctttctt ttatgaaaac
    13561 gatgattgct agtttctgcc tttctctatt ttttgaattg acgcagctaa gtggacttta
    13621 ttttatctac ccaagaccat accgcttatt tgatgtgaat gatttagtga caaatacatt
    13681 gggtggaatc attggttttg tcgttacgcc gttatttaca tttatgcttc ctacaagaag
    13741 acgaatggat gaactttctt atgaaaaagg aaagactgtc tcattgactc gacgcttggt
    13801 cgcttatttg atcgactgga catttttatc tcttattaat ttggtagcgg cgattttctt
    13861 taaaattgcg ttcgattcag gggcagttac ttctcatatc tggtggcttt tattagaagt
    13921 aggggtatac tttatgattg taccgtttat aaccaatgga tggacacttg gcaaaaaaat
    13981 tgtgcgtatc cgtttggtgg aagaagaccg cgaacgaatt tctttaaaag cattgatgat
    14041 ccgctacggg tatctgtact ttctttttta tggtttaagc tggttggggc agttgcttca
    14101 gcaaatcatg aattcgacaa cgaatacggt gttgcagatg agtgcagtat tcatttctct
    14161 cggtgtcttg ttactacaag gtatttttat tttaaatatc ttgctatcat ttttgagaaa
    14221 aaaacggatt ctgttttatg aaaaagctag tcacacatat actgttagca cgattggcca
    14281 aacaaaggat gatgattctc gaaattagct gagcaaaaac gctttgtctg tgttataatg
    14341 ccaaagtcgt gataatttaa agagaagagg tgcacgaatg aataattttg atatattccg
    14401 cttcaaaccg tttatcaatg aagcgttaac cgcaaagggc ttcaaacagc ccacagaggt
    14461 ccaggaacgt ttgattcctt taattgcaaa aggaaaaaat atcattgggc aatctcaaac
    14521 tggtacagga aaaacacata cgtttttgtt gcctttgttt gaaaaaatca atgcagaaaa
    14581 agaggaagta caagttgtta ttacagctcc aagtagggaa ctagctgcac agatctatga
    14641 agcagctaca caaatcgcta aattttctac accggagatc cgtgtctcga attttgtcgg
    14701 cggtacagac aaacagcgtc aattaaatcg tctgcatcac caacagccac aggttgtagt
    14761 aggcacgcct ggtagaatat tagacatgat gaatgaacaa gcgctgaaga ttcacaccgc
    14821 atttgcattt gttgtcgatg aagcggatat gacattggat atgggttttt taaatgaagt
    14881 agaccaaatt gcaagtcgat taccagaaaa gctgcagttt ttagtttttt ctgccactat
    14941 tccagaaaaa cttcgtccat tcttacgaaa atacatggaa aatccaatca ttgaagaaat
    15001 caaaccaaaa gcagttattt ctgaagacat tgaaaattgg ctgatttcaa caaaaggtaa
    15061 agatgagaat caaatgattt atcaactgct gacaattggt catccttatt tagcaatcgt
    15121 atttgccaat acaaaacagc atgtggatga aattgccgat tacttgaaaa accaaggctt
    15181 gaaagtagca aagattcatg gagacatcac cccaagagag agaaaacgag tgatgcgaca
    15241 agtacagaat ctggagtatc aatatgttgt agcgactgat ttagctgcta gagggatcga
    15301 tatcgaaggt gtctctcatg ttattaatgc agaaattcca gaagatctag acttctttat
    15361 ccatcgtgtc ggacgtactg gacgtaatgg cttaaaagga acggccatta cgttatattc
    15421 gccaaatgat gagcaagcca ttgatgcgat agaaaaattg ggcgtctcat ttgaaccaaa
    15481 agaaatcaaa aacggggaaa tcgtcaaaac atacgatcgt aatagacgtg ccaaacgaga
    15541 aaaatcaaaa gatgtattag atcccacgct gattggtctt gtgaagaaga aaaagaaaaa
    15601 gatcaagcct ggttataaga aaaaaatcga ttgggcaatt gcagaaaaaa ataaaaaaga
    15661 acgcaaaatc gaacgcagac agcaaagccg tactgctcgt aaaaataaga aaaattcaca
    15721 taaataaaaa acaagaggtt gtgacaaaag gcgtgtcaca atctcttgtt tttaagctag
    15781 actttttttc attccgatac aaagtacttc atcttcaata aaaggttctg tgcaagtatg
    15841 atacccttgt ttttcataaa aatctacagc cgtcatttca gctactacat aactagtctg
    15901 acaaccttct gaaaccgccc gtttttcagc agcttcaagc aactgaccgc ctaacccttt
    15961 tcctcggtat tcttttgcta cgcaaaatcg ttccggcttg aagcaagtag gtgttttctt
    16021 ttcataacgc aaagtagcta ctggtctttt ttgatcaaga aatagataat aggtttgttg
    16081 cgggatatct tttccgtcga attctagttc tgcaggaatc ccttgttcta atacaaaaac
    16141 ttgatatctc aaaaagcagc tagctgcgcg tatccattca tccgctccaa ctattttttt
    16201 catcatattc ctccttgtca tgaatagcag agatgtattg attcctttat tcttatcgta
    16261 ccaaaaatcg tggagtatga ccaaaaggaa atgaaaataa aaattgagat attgacagtg
    16321 atcaatagaa tgcgtatact aattaagtac gaaaaggata tgatttgttt tttaagactc
    16381 acagaaaatt ctttcttggc tgaaaaaaga atagtcacta atcaaattgc tccctttttc
    16441 tattttggta gaaataccga cgcagctcag cgttatcgag tttaagaggt aatgattaca
    16501 catcattgca atcaaggtgg taccgcgagt tttcgtcctt ggctgtgatg gtgttttttt
    16561 gtatttaatt tatagaaagc aggtaatgac atgaaacaat taagtagtgc acaagttcga
    16621 caaatgtttc ttgatttctt ccaatctaaa ggacatacga ttgaacctag tgcgtctttg
    16681 atccccgtga acgatccaac cttattgtgg atcaactctg gtgttgcaac attgaaaaaa
    16741 tattttgatg gttcagttgt gccagaaaat ccaagaatca caaatgccca aaaatctatt
    16801 cgaacaaatg atatcgaaaa tgtcggtaaa acagcacggc accatacgat gtttgagatg
    16861 ctgggtaatt tttcgattgg tgactatttc aaaaatgaag ccatccactg ggcatgggaa
    16921 tttttgactg atccaaaatg gatggcattc gatccagaaa aactttatgt aacagtttat
    16981 ccaaaagata cagaagcaaa acgcatctgg catgaagaag ttggcctgcc actagatcac
    17041 attattgatg tagaagacaa cttctgggat atcggtgcag gcccatgtgg tccggatacg
    17101 gaaattttct atgaccgtgg agaagcttat aacgatttag cagaagacga tcctgaaaac
    17161 tatccaggag gagaaaatga acgttattta gaaatctgga acttagtatt ttcagaattc
    17221 aaccataagc ctgataacac atttgaacca cttcctcata aaaacattga tacggggatg
    17281 ggattggaac gcatggtctc aatcgttcaa gatgcgtcaa cgaactttga gacagacttg
    17341 tttatgccta ttatccacgc cgtagaaaag ctaagcaaac aagtaaaata tggtgaaaac
    17401 ccagtaacag atatttctta caaagtaatc gcagatcata ttcgcgccct ttcatttgct
    17461 attggcgacg gtgcattgcc atcgaatgaa gggagagggt atgtcttacg ccgtttgctt
    17521 cgtcgtgctg tcatgcatgg gaaaaaatta ggtattgaac aagcattctt atatcgttta
    17581 gtccctgttg ttggggaaat catggtaagt tattatcctg aagtacttca acaaaaagaa
    17641 tttattgaaa aagttatccg tacagaagaa gaacgtttca atgaaacaat caatgaaggt
    17701 ttggaaatct taaatcaagt gatccaagaa gtaaaaaacg aaaacgggac gactctagca
    17761 ggaaaagaca tcttcaaact atacgacaca tatggcttcc cggtagaatt aacggaagag
    17821 gtggcagaag atgaaggact gaaagttgat catgctggtt ttgaaaaaga aatggatgca
    17881 caacgcgaac gtgctcgctc tgcccgcagt actgaaaaat caatgggggt acaatctgct
    17941 ttattgacag atatcaaagt agaaagtaaa ttcattggat acacagaaca agaatcagat
    18001 agtcaattat tggtcatcgt taaaggtgag tctcttttga atgaagtcgc agaaggtgaa
    18061 gctcaaatga tctttgatca aacacctttc tatgcagaaa tgggtgggca agtagcggat
    18121 caaggatgga tcatgaatga agtaggcgaa atcgtagcag ttgttacaga tgttcaaaaa
    18181 gcgcctaatg gtcaatttat gcacaccgta gaagtaaaag caccaatgag tgaaggacaa
    18241 acctatcatc tattgatcga tcaatcgatg cgcaatcgga tcatcaaaaa ccatacagca
    18301 acccatctac tgcacaaggc attgaaagaa gtacttggcg agcatgcaaa tcaagctggt
    18361 tctttagtcg ctcctggaca tttacgattc gactttaccc actttggtca aatcacttca
    18421 gatgaattag ctgaaatgga aagaatcgtc aacgaaaaaa tctgggaagc tatcccagtt
    18481 gaaacaattg aaacagatat cgatacagcc aaaaacatgg gtgcaatggc tttgtttggt
    18541 gaaaaatacg gacatgacgt tcgagtagta aatattggcg gctggtcaat cgaactgtgc
    18601 ggaggcacac atgtagcaaa tacagaaaac atcggaatct tcaaaatcgt ttctgaatca
    18661 ggaattggtg caggcgtgcg acgaatcgag gctgtaacaa gtaaagaagc gtacgaacta
    18721 cttgaagaag aagaaaaaca actaaaacaa gtagcaacaa ttgttaaatc acctcaattg
    18781 aaagaagttg tttcaaaagc ggaacaattg caacagcaag taagagagct acaaaaggaa
    18841 aatgaacaat tagctagcaa gttagcgaat caacaagctg gtgatgtatt caaagatcca
    18901 gaaacgatca atggaaaaac aatcattgca gcacaagtaa aagtgaaaga tatgaatcag
    18961 ctgcgtcaat tagctgacca atggaaacaa aaaaatattt cagatattct tgttttagca
    19021 aacgaacaag aaggaaaagt cagtctgcta gcggcaatga cgaaagaatc aaatgaagct
    19081 ggactaaaag ctggcgactt gattaaagca atcgcaccga aagtaggcgg tggcggtggt
    19141 ggacgtccag acatggcaca agctggcggg aaaaatcctg ctggtattcc agatgcactt
    19201 aaagctgcga aagaatgggt tgcgcaacaa tagggcgttt acgtttgaca gaaaaacaaa
    19261 agttcaaaca ttgaacgaaa catgattatg ggggcgaaga caaacaagtc ttgccctcat
    19321 acttttttca attggatggc gcatctaatc aaatggtcgg attcggatgg tctttagttg
    19381 acgaaagtga cagaaatttt tacgattagt ataaataaaa aggggtgaaa aaatgaatag
    19441 tagaaataag acctttcgac tagtcttacg tgcgatcttg ttagcaatca tcatcgtaca
    19501 agcgatggtt ccttggctgg ggtttattcc attaggattc attagtttaa caattatcca
    19561 tatcacagta attattgctg cagtagtatt aggcccaaaa gatggcatgg tgattggctt
    19621 gttttgggga atcgctacaa ttgttcgtgc ctatgccatg ccaacaacgc catttgatac
    19681 attagtgttc acgaatccga ttatctcggt agttcccaga gtacttgttg gcttagtagc
    19741 aggcttggtt ttccactgga tttatcaaaa aaataaatca ataacgatca gctcagcctt
    19801 tgcaggggta ctgggttcat tggtcaatac agtcttagtg ttaggattca tgggattatt
    19861 ttatacaggt gcaacggcag atgcctatgg tgtagactct gctcttttat ttaaaacctt
    19921 agcaggggtt gcagcaatca atgggatacc agaagcaata ggtgcgggaa tcataacacc
    19981 attactagca aaagcgttat ttgcagcaac accgttgaaa ccagaataat gaccatcttt
    20041 taatagtaat aggacgcacg atagatagaa aaaggaagag aggagaggaa aacaatcgtc
    20101 ctctccatct ttttacctga gcaacaagca aactagaagg ggtaaaaaac ttggctaaga
    20161 aacaagaaga aaaaaagaaa caaataccgc aacagcggac aaaaaaagga gtactagcgg
    20221 gtatcttagg attggttacg aatctgatct tgtttgtctc taagctaatg atcgggcttt
    20281 tttcaggaag tgtctccatt atggctgatg cagtgaacag tctatcagat actgcttcat
    20341 ccatattgac tttgatcgga tttaaaattg ctgcaaagcc agcagatcag gaacatcctt
    20401 atggacacga acgcttcgaa tatatcagtg ggttatttgt ttcgatcatc attacttatg
    20461 taggatttca atttttagag tcttctgctg aaaaaatcat ccatccagaa aatgtctctt
    20521 taacacccat cgtctttttt gtcctaatca tttccatctt tttgaaattt gcacaaggca
    20581 aaatgtatca ggttattgcg caatccattg attctgaaac attgagagct acagcaacag
    20641 atagttataa tgatgtcttt actacactgg ctgtattgct ttcagcagca atcgaatggg
    20701 taactggatg gcgtattgat ggatatatag gttttgcttt agcattatat atcctttata
    20761 gtggattgac gatgcttcga aattttgtat acgaactatt aggaagtcgc ccaacgccag
    20821 cagagattga agaaatggaa gagcatttaa atgcttgcga tgatatactt ggctatcatg
    20881 atttattggt gcataactat ggacccaata aacgctttgc atcagtccac atcgaagtag
    20941 atgaatcaat gaatttgaat gtggctcata agattattga tctgatagaa aaagacttta
    21001 aaaagtcttt ggatgtggaa cttgtctgtc acttggatcc aatgcctgtt gataacgaaa
    21061 aatactataa tatcttgcgg cagttaaagc aaattcttct tgaaacaggt acagggctta
    21121 atattcatga ttttagagta attcatcaag gaaaaacgct gcaatttgat gtagtggtcc
    21181 ctgagaaatt ccagtttgtt gatacagaac tagaaagaat cttacgtagt caaatagaag
    21241 aaaaaatcgg agattaccaa ttggaaatta cgtttgatca caattatctt ttatattaaa
    21301 tgagaaacta taaaaagact ccttaaagtt tttaatttaa agagtctttt tttcttttaa
    21361 ttgtttatgg agagcctgac gtgcaagatt atctgctcct ttatttttgt tttcgggcaa
    21421 ccattgcagg atcaataaag aaaattcgga tagaagttct tggattcttt gaaggtaggg
    21481 aaggaagagc tcgttcttcg ttgcgttttt gtcgaccgta gaaactagtg ttttgctgtc
    21541 tgaaaaacag aaaatcgttt cattctgtaa gtcgtttttc tttagaaatt ctaaagtttt
    21601 taaaaagact tcgaactctg cttgatggtt tgtccctata gaaagcggga tagatagctg
    21661 ttcttgttta ccttgataga caatcaagat tccacctcca ctaggaccag ggttgccttt
    21721 tgtagaggca tcaatgtata cttttaacat aaaaagagat tctccttttt gttcaaacta
    21781 atgttatggt aagattatat cattactgaa tcggtgagag gttagagaat gaaaaaaaat
    21841 attcgatggc agcctgaact ggcacatacg atcatctatt ggtcattgac cttgatcatt
    21901 ctgtttttta gtctgattct ttctttagaa aacacaggtc cttattggaa aagcaatttt
    21961 gttttaggta tttttcttgt ttttctattt ctaggtttgc ggcgttcttt tattttaaaa
    22021 tcaaactctg tgattatacg ttatgctgct ttttggaaag accgagaaat atctgtcgga
    22081 cagattcgat ctttgacgcc aactaatact ggggtgcaaa tgatgttgca agatgagaaa
    22141 gccccatctg ttttcatgat gcgaaaaaaa gatcaagtag tcctttttga tcattttgaa
    22201 aaaaacggac ccaaatcaga atcaattcct tatccttata cgaaaaaaag aaaaagctaa
    22261 ttttaaaaat cagaacctaa aaaaacgaat ttttacagga aaagaaaaga gtgagacgta
    22321 cggttcatcc cgtttagcgt ctcactctat ttgtttatct attcatgcat actagtatcg
    22381 gtagtctgaa cgttgccttt cttgacatgg gcagcttgtg ttcctcgatt cccttcgatg
    22441 atctcaaatt cgaccacttg attttcttcc aaagttttga atccctcttc ttcaatcgca
    22501 gtaaaatgta caaaaatttc ttctgtgtca tcataactga taaagccgta tccttttttg
    22561 ttgtcaaacc attttacaat tcctgtggtc aaagcactca cgacctttct aaagtctgat
    22621 catagccttc attataaaga gtatttataa gtacgtcaaa taaattaaaa acctgtaatt
    22681 ttatgttttt tttctgaata aaaaaaggta taattggcat atcttaacaa agaagatatt
    22741 ttgttgaata aagttcgtga aaaggggtaa tcaaatgaag aatgatctgc aaatttcaca
    22801 agaaacagag ctagaaccga ttgttgaagt ggctagtaaa ttagatctta aggaagacga
    22861 tttggaactg tatgggaaat ataaggcgaa aatcaatttt tctggaatca atcgatcaaa
    22921 ggataacgct gaggggcatt tgatcttagt tacttccatc aatccaacac cagctggcga
    22981 aggaaagtca acgatcactg tagggttagg agacgcgctc aataaaatca ataaaaaatc
    23041 tgttattgca ttgcgagaac cctcacttgg tcctgtgatg ggaatcaaag gtggtgctgc
    23101 aggcggagga tatgcacagg tattgcctat ggaagatatc aacttgcatt ttactggtga
    23161 catgcatgct atcacaacag ctaataatgc attatctgct cttttagata atcatattca
    23221 tcaaggaaat gaattgaaca tcgatgcccg ccgagtgatt tggaaacggg tagtcgatct
    23281 caatgatcgt gaactgcgta aagtaattgt tggcttgggt gggccaatcc aaggtgttcc
    23341 aagagaagat gggtttgata tcacagttgc cagcgaaatc atggctatct tgtgtcttgc
    23401 ggcagacctt gaagatttaa aggctcgtct tgcaaggatc gttgtcggct atacatttga
    23461 ccgtcagcct gtgactgctg gagacttgaa agcagaaggc gctctggcat tactgctgaa
    23521 agatgcaatc aagccgaatc ttgtacaaac aatttacgga acaccagctt ttgtccacgg
    23581 gggaccgttt gccaatatcg ctcatggctg caatagtatt ttagctacca agacagcatt
    23641 gagattagca gattacgttg tcacagaggc aggatttggt gcagatcttg gcggagagaa
    23701 atttttagat atcaaagtac ctaacttgaa aaaagcgccg gatgctgtgg tgattgtcgc
    23761 cactgtccgt gcattgaaaa tgcatggcgg aatgaaaaaa gatgaactta aaaatgagaa
    23821 tcttgatgct ttgaaagtag gttttgcaaa tttaaagcgc catatacgta atatggaaca
    23881 atatcagcta cctgtgatcg tcgcaatcaa tgaattcgta acagatacag acaatgaact
    23941 cacactgttg gagcacttgt gtgaaaatca ggggattctt gccaagagag cgagtgtatg
    24001 ggcagatgga gcagaaggcg gcgttgattt agcaaaagct gtagtacgat tgattgatag
    24061 aaaagaagct aattacaagc cattataccg cctagaagaa acgatacaag aaaagacaga
    24121 aacaatcgtc aagaaaattt acggaggaaa tggtgttgtt ttcagcaaaa aagcgcaaaa
    24181 acaaatagaa gagtttacga agaatggatg ggacaaactg ccgatttgta tggcaaaaac
    24241 acagtattca ttttctgatg atccttcact gctaggtgca ccggaagatt tcaccatcac
    24301 gatccgcgaa atcatcccaa aattaggggc aggattttta gtagccttga ctggagatgt
    24361 tatgacgatg cctggtttgc ctaaaaaacc tgctgcattg aatatggacg tgacaaatga
    24421 cggagaagtg ttgggattgt tttaagcttg attataatct accatcgaag gagctgtatt
    24481 ttgactacag cttctttttt tagacgatag ggaattaatt ttgtttacac taggatcatt
    24541 ctttttcttt attgtatttt gagctatact aaagcataat aggagggtat gatgaaaacg
    24601 acatttataa gaaaaaccgg tttttacggt atgggtagct cgattcagct aagaatcaac
    24661 aatgaaaaac gcagtgttct cagtcatgaa caaaagataa cgcttgatct ggaagtgccc
    24721 tttacgatgc aggtcacctt ttattggcta aaaagccccg tttatacagt gaaagaagca
    24781 aagcctgctt atgtgatcac aatgaattac ttgattcttc aaatctatcc gtttttattt
    24841 ctagcaactg ggttgggggc cgttttccta caaaatttat ttttcagttt ttttgcagtt
    24901 attgtgttga ttgcgttctt tttctatata aaaaaccggg catatttgat caaggaggaa
    24961 aattatggca aactctgatc tttttttaat cagtttcaat cgaatcgaaa aatggatgag
    25021 agacgagatg ggaaatgcac gaaatatggg atttaccgaa cttgtcagac gcctagctca
    25081 aagaaaacaa ttaatgatca gaaaatacga agatgatctt ttacagcttg ctcagttaag
    25141 gaatgcgatc gtccatgatc ggatcgcagt cgattttgtc attgcagagc caaatgattg
    25201 gacagtcaag cgaatccagc tgatcgaaca agaactgatt cagcctgaaa ccgtgctgcc
    25261 tcgatttgca aaacgtgtaa caggttttga acaagatctt ccgttacttg aactgttaaa
    25321 gatagtagca gaaaaaaggt attcacaatt tccgttatac aataaaggaa aatttgtagc
    25381 attgattact ttacgtaata tcgggttttg gctagcaaaa gaaagccaaa aaggtccggt
    25441 tgatttaaca aataaacgag cgttggattt gatcatccaa aacggaaagt acacaaatta
    25501 tcattttgtt ccagctaaaa cacatattta cgaggttgaa gcgatgtttc gtgagcaagg
    25561 gactttggaa gccgtactta tcactaaaga tgggaatccc gatggtaatc tgctcgggat
    25621 cgtacgacct cgtgatattt ataaacaaat agaaaaggat tgattttttg ttaattattt
    25681 attggatatt cagcgctttg ctagtaggag tggatcagtt ggtcaaatgg tggatcactg
    25741 ataatctagc gcttggagag acgaaaaatc ttataccagg tattttgtca ctgaatcata
    25801 ttcgaaacac tggagccgca tggagtatgc tagaagggaa gatgtggttc tttactatag
    25861 tgacactcat tgcagtcgtt gtgattctca cattgatgat taaaaatcgt gaaaaaggta
    25921 atcgctggtt catgattgga ttgagtttga ttctagctgg tgcgattggc aattttatcg
    25981 accgtattcg tcttggctat gtagtagata tgttccagac agattttatg agctttccga
    26041 tttttaacgt agcagatgtg acacttgtat gtggagtgat ttgtatgtta atttatatta
    26101 ttttagatga aaaggatcaa agaaaaaaat gacagaactt aaaacgacga ttcaccatga
    26161 atctggtagg atcgataaag tattgactaa tttattcacc gattacagtc gttcgcaaat
    26221 tcagctgtgg ctgaaaaatg gagcggtttc tgtcaatgga cagccagtca aagcaaatta
    26281 caaagtaaaa caaggagacg agattactgt cttgatcccc gaaccagaaa cattagatgt
    26341 ggtcccagaa aatatcccgt tgacgatcgt ttacgaagat gaagatgtag cggtgataaa
    26401 caaacctcaa ggaatggtcg ttcatccttc agcaggtcat acgcatggca caatggtcaa
    26461 tgcgctgatg taccatatca aggatctttc tacgatcaac ggcgttattc gtccaggtat
    26521 tgttcaccga atcgacaaag atacttccgg actgctaatg gttgccaaaa atgaccaggc
    26581 acacgaatca ttagctcagc aattaaaaga taagacctct ttgcgcaaat atgttgcttt
    26641 agtccatgga gagattcctc acgaaaaagg aaccatcaat gcgccaatcg gccgttcgaa
    26701 ggaaaaccgg aaaatgcagg cgataagaga agatgggaag tcggctgtca cacattttac
    26761 tgtattggaa cgtttcgaag gatttactct ggttgaatta aaactagaaa ccggaagaac
    26821 acaccagatc cgtgtgcata tgaagtatat cggttatccg ttagctggag atccagtata
    26881 cggaccgaag aaaactttga aaggcaatgg tcagtttctc catgcgcaaa tgttaggttt
    26941 tacgcacccg actacaaacg aaaagatgat ctttgaagca cctcttccag aagtttttga
    27001 aaaaacttta gaaaaattaa gaaaaaacat tgcattttaa tttagataac ggtatagtaa
    27061 ttaacaacga aagcataaaa cttaatagaa ttcctttaag tttagtcccg tgaggctaag
    27121 aagggtggga gcaaggatac gtatggtgcg ccataagtag attgctcatt gacatagtat
    27181 agctatcagt gtatcagtat acgctcagtc taaacattcc tcctgtcctc ttagggcagg
    27241 agttttttta tgcaaaaaag tagggggaat gaaaatgcaa gcaaaagaag tagtagatca
    27301 ggtaaccatg aagcgagcat tgactcgaat tacttatgag attatcgaga gaaaccatag
    27361 tatccaagat atcgtattgg taggtatcaa aacacgagga atctacattg catcaagaat
    27421 tgccgaacgc ctgaagcagc tagaagatat cgatattcct gtaggagaac tggatatcac
    27481 tttataccga gatgacaaaa aagaaaatcc agaagaaccg gaacttcatt cttctgacat
    27541 cccagtttcc ttagaaggaa aagaagttat tttgattgat gatgttttgt atacaggacg
    27601 aacgattcga gcagcaatgg acgcagtaat ggattttggc agaccaagaa aaatttcatt
    27661 agcagtcctg gtcgatcgtg ggcatcgaga attgccaatc cgtgcagatt atgttgggaa
    27721 gaacattcca acatctcggg cggaagagat tttagtagag atgcaagagc ttgatggaca
    27781 agatcgcatc atgattttga aagaggaaga ttagaggaga gagaaacatg tcggagaaaa
    27841 aaggatttca taatgatgac gtggtgttag atatccacga tcgcccaacc ccaggacatt
    27901 gggtaggatt aagtttacaa cacttgttca cgatgttcgg agcaaccgtg ttagtcccca
    27961 tcttagtagg tatcgatcca ggtattgcgc tagtcagttc aggactaggt acattggtgt
    28021 acttatttac tactaaagga aagatacctg cgtacttagg aagcagcttc gctttcattg
    28081 cagcgatgca gcttttgatg aaaacagatg gttatccagc gattgcacaa ggagcagtca
    28141 caactggatt agtctatttg attgttgcgc tgatcatcaa aaagatcggt tcagcatggc
    28201 tggataaaat cttgcctcca atcgtcgtcg gtccagtcat catggttatc gggttgggac
    28261 ttgcggcaaa tgcagcaaca aatgctatgt ataatcaggg cacgtatgat ttcaagtata
    28321 ttgctgtagc gctgatcaca ttaggattaa caattttctt caacatgtgg ctaagaggtt
    28381 ttctcggatt gattccgatt ttactaggta ttattagcgg atacatcgta gccttgctgt
    28441 ttggaatcgt ggatactaag ccaatcgtag acgctgcctg gtttgctgtt cctaactttg
    28501 agattccctt tgtacaatat gaaccaaagc tatatcttgg agcaatcaca acaatggcac
    28561 ccatcgcttt tgtgacgatg acagagcata tcggccatct tatggtattg aataaactaa
    28621 ctaaacgtaa tttctttgaa gaaccgggtc ttcataagac gttgacaggt gatggattag
    28681 cacaaatcgt ggcagccttt gtcggaggtc ctcctgtaac aagttacggt gaaaacattg
    28741 gtgttctggc tatcacacgt gtccatagcg tgtttgtgat cggtggagct gcagtatttg
    28801 cagtcatttt aggattcgta ggaaaattaa gcgctttgat tttaagtatc cctggaccag
    28861 tcatctcagg aatcagtttt atcttatttg gagtaattgc cgcaagtgga atgaagattt
    28921 taatcgagaa caaaatcgat tttgataaaa agaaaaatct gttgattgct tcagttatct
    28981 tagtagtagg aattggcgga ctcgtctttg aagtaggcac atttacattg tcggcaatgg
    29041 cattagcaac tgtacttggc atcattttaa atcttatctt accagaaact tcacgaagcg
    29101 aagaaaacta aggaggaaac attatgatca tcaaatcaga acgtatcagc ttaaagcatt
    29161 tattgaccgt tgaggcactt actgatcaag aagttatggg cttgatccgc agagggcagg
    29221 aattcaagca aggtgctaat tggactccgc aaaagcgcca gtactttgct acaaacctct
    29281 ttttcgaaaa tagtactcgc acacataaaa gcttcgatgt agcggagaaa aaactagggc
    29341 tagaagtgat tgaatttgaa gcaagcacaa gctctgtcca aaaaggggaa acactatacg
    29401 atacagtact gacgatgtca gcattaggcg tagacgtagc ggttatccgt catggtgatg
    29461 agaactatta tgacgaactg atacaaagta aaacaatcca gtgttcgatt atcaatggag
    29521 gagacggaag cggacagcat ccaacccaat gcttgcttga tttgatgacc atttatgaag
    29581 aatttggtca ttttgaaggt ctgaaagttg ccatcgtagg tgatatcact cactctcgcg
    29641 tagctaaatc caatatgcag atgttaaaac gtctaggcgc acaagtattc ttctcaggtc
    29701 ctagagaatg gtacgacgat gaattcgaag tgtacggaca ttatatgcca ttggatgaac
    29761 tactagatca agttgatgtc atgatgatgc ttcgggtgca acacgaacgc catgatggaa
    29821 aagaaagttt ctcaaaagaa ggctaccatc aggaacatgg gttaacagtt gagcgtgcga
    29881 agaagttgca aaaacatgca atcattatgc acccagcacc agttaaccgt gatgtagaat
    29941 tagctgattc attagtggaa ggattacagt cgcggatcgt tcagcaaatg agcaatggag
    30001 tattcgtcag aatggcaatc ttagaagcag tgctaaatgg aaaagcgtaa ttataggagg
    30061 atgaaaaaat gaaaacactc attaaaaacg gtcagatcaa tacacgaaaa aatatgacga
    30121 caccagcaga aatttggata gaagatgggc gaatcaaagc aatcggtaca ggattctcag
    30181 aagccgaatt tgatgaagtg tttgatgcaa agggacaatt gatcacacct ggtttagtgg
    30241 acgtccatgt tcatttgaga gagccaggat ttacatacaa ggagacgatc gaagctggga
    30301 ctcgctcagc tgctcgtggc ggtttcacta ctgtatgcgc gatgccgaat ttaaatcctg
    30361 tacctgatac ggcagaaaag ctgagacaag tatatgacat cattagaaaa gatgcagttg
    30421 tgaaggtatt gcagtatgcg ccaatcacag aaaatctgcg aagtgagaaa ttagttgatc
    30481 aagaagcact gattgaagaa ggtgcttttg ctttcacaaa tgacggtgta ggtgtccaaa
    30541 cagctgggac gatgtatctg gctatgaaag aagctgcaaa aaacaataaa gccctagtag
    30601 cccatacaga agatgaatct ttattatttg gcggcgttat gcacgctggc aagaaggcag
    30661 aagaacttgg tcttcccggg atcttaagcg taaccgagtc ttctcaaatt gctagagatc
    30721 tgttgctagc tgaagcaact ggtgttcatt accatgtttg ccatgtttct actaaagaaa
    30781 gtgtccgtgt aatcagagac gcaaaaaaag caggaatcca cgtaacagca gaagtttcac
    30841 cccatcatct tattttgatc gacgaagata ttcccgaaga tttcggcttt tggaaaatga
    30901 acccgccgct aagaggacgg gaggacagag aagctttgat cgaaggcttg cttgacggta
    30961 cgatcgattg catcgctaca gaccatgccc cccatggatt agaagaaaaa agccaaagtt
    31021 tcatgaaatc accatttggt atcgtaggaa gcgaaacagc gtttcaactg atctatactc
    31081 attttgtaga aactggtcga ttcacgttgg aacaagtgat caattggttg gcagttaaac
    31141 cggctgagat ttttggtttg aatgcaggaa cgctaacggt tggtgccccc gctgatgtag
    31201 cagtttttga tattacacaa acttgcacga tcgataaaga agacttttta tcaaaagggg
    31261 agaatacacc ttttatcggc tggaaagtaa aaggcgaaac acaaatgact tttgtcaacg
    31321 gaaaacttgt gtggcagaag ggagaataaa agtatggaac gtttgcttat attagaagat
    31381 ggaacagttt tcgaaggaaa ggcttttggt gcagatgcta gtgtcttcgg agaaatcgtt
    31441 tttacaacaa gtatgactgg ttatcaagaa actatcactg atcaaagttt taacggtcaa
    31501 atcattacat ttacctatcc tatggtgggt aattatgggg taaatagaga tgattatgaa
    31561 tcgatttctc caacatgcaa aggagtagta gtaaaagaac atgcacgtgt agcttcaaac
    31621 tggcgaaacc aaatgacgct agattcattt ttgaaacaaa aaggaattcc tggaatatca
    31681 ggtatcgata cgcgcgccct tactcgcaaa atccgtgaag tcggaacgat gaaagctagt
    31741 atcgttgatg ctggagattc cttcgaacat gcatttgatc agctgaaagc aaccgttcta
    31801 gcaaccaatc aagtgcagca agtctcaact aacaaacctt atccaagtcc aggaacaggt
    31861 cggaaagtca ttgtcattga cttcggcttg aaacatagta ttttacgaga attgagtaaa
    31921 agaaactgta acctcacagt acttccctat aacacagatg ccgagacgat tttagcgcta
    31981 tcaccagacg gagtcatgct gacgaatgga cctggtgatc caaaagatgt gccagaagca
    32041 atcgaaatga tcaaaaaaat ccaaggaaaa gtaccgattt ttggtatttg tctcggtcac
    32101 cagttatttg ctttagccaa cggtgcagat acctataaaa tgaaattcgg acatcgagga
    32161 ttaaatcacc cggtacgaga agtagcgacc ggaagaattg attttacttc tcaaaatcat
    32221 ggctatgcag tcgatgaagc cacgattgat ccagaaaaac tcatcgtaac tcatgtagaa
    32281 gtaaatgacg gaacagtaga aggagtcaaa cacagagatt atcccgcttt cagtgtgcag
    32341 tttcatccag atgctgcccc tggtccgcat gatgcactac atttatttga tgaatttatg
    32401 gaaatgatgg acgcaggaaa ggagcagtaa acagatgccg aaacgtacag atatcaagaa
    32461 aattatggtg attggatctg gtccgatcat cattggtcaa gccgcagaat ttgactacgc
    32521 aggaacacaa gcctgccttg ccttaaaaga agaaggatat gaagtggttt tagtaaattc
    32581 taatcctgcg acgatcatga cggataaaga agtggcagat caagtatata tcgaaccgat
    32641 cacacttgaa tttgtatcaa ggattcttcg aaaagagcgt ccagatgctc tccttccaac
    32701 tttaggcgga cagacaggcc tcaatatggc aatggagctg gcagcatcag gtattttaga
    32761 agaattgaac atagaacttc taggaacaaa gctttctgcg atcgatcaag cagaagatcg
    32821 tgatctattc aaacagttga tgaaagaatt gaatcagcca atccccgagt ctgaaatcgt
    32881 caatacagta gaagaagctg tagattttgc aaataaaatc ggctatcctg tcatcgttcg
    32941 tccagccttt actttaggcg gaactggtgg aggtatgtgt gaaaatgaag aagaattacg
    33001 tatcgtatca gaaaatggat taagtctttc ccccgctacc caatgtttga ttgaacgaag
    33061 tattgcaggt tttaaagaaa ttgaatatga agtgatgaga gattcggctg ataatgcaat
    33121 cgttgtctgc aatatggaaa attttgaccc ggtgggtatc catacaggtg attctatcgt
    33181 atttgcccct agtcaaacat tgtctgacca tgaatatcaa atgcttcgtg atgcttctct
    33241 ttcgattatt cgagcactta agatcgaggg aggctgtaat gtccaactcg ctcttgatcc
    33301 acatagtttt aattattatg tgattgaagt caatccgcgt gtttctcgtt catctgcgtt
    33361 agcaagtaag gcgactggct atccgatcgc taagctagct gctaaaatag cgcttggatt
    33421 aacattagac gaaatgaaaa atcctgttac aggaacgact tttgcagaat ttgaacctgc
    33481 attagattat gttgtagcta aaattcctcg ctggccgttt gataagtttg aaaacggaga
    33541 acggctttta ggcacgcaga tgaaagcaac tggtgaagtg atggctatcg gtcggaatat
    33601 cgaagagtct ttattgaaag ctgtgcgttc tttagaaatc ggtacacacc atatcgaact
    33661 tcctgagttg gcagacatca cggacgatga cctaatccaa aaagtggttc gagcacaaga
    33721 tgatcgcctg ttttatgtag ctgaagctat tagaagaggg tacaccatag aagaattagc
    33781 ggatttgaca aaaatcgatt tattcttttt agataaactg ttgcatatcg tagaaatcga
    33841 acagcagttg aaacagcagc cgttggatga caagatattg aaaacagcga aacaaaacgg
    33901 ttttacagac aaaaaagtgg cggagctgtg ggaaataaac gaagaagccg ttcgccagat
    33961 gagaaaagaa aatgatttga ttcctgtata taagatggtt gacacttgtg cagcggaatt
    34021 cgaatcaaaa acaccttatt tttatagttc ctatgaatat gaaaatgaaa gttcacgatc
    34081 agaaaagccc tcaattctag tactgggatc tggtcctatc cgtatcggac aaggggttga
    34141 atttgactat gcgactgtgc attctgtcaa agcaatccag caggcaggat acgaagcaat
    34201 catcatgaac agcaatcctg aaacagtttc gactgacttt tctatttcgg ataaattata
    34261 ttttgagcca ttgaccttcg aagatgtgat gaatgtgatc gagctagaac agccgatagg
    34321 agtaatcgtt caatttggtg gtcagacagc aatcaactta gccgaacctt tgactgctgc
    34381 tggtgtaaaa atcttaggga caacgatcga agacttggat cgtgcggaaa atcgtgattt
    34441 attcgagcaa gcgctgaaag agttggatat tcctcaacct ttaggggata ctgctacaag
    34501 taaagaagaa gccgtggtta tcgcttcgag aattggttat ccagttcttg ttcgtccaag
    34561 ctatgtatta ggcggacgtg cgatggaaat cgttgaaaac cagcgagatt tagaagatta
    34621 catggaacat gctgtgaaag catctccaga acatccagta ctagtggacc gttacttgct
    34681 tggtggtgaa tgcgaagtgg atgcgatttg tgacggtgaa acagtactca tcccagggat
    34741 catggagcat attgagcgtg ctggtgtcca ttcaggggat tcaatggctg tgtatccacc
    34801 tcaaacatta tctgaatcga tcaagaaaac gatcgaagat tacacgatcc gcttagcatt
    34861 aggtttgaat tgtgtcggga tgatgaatat ccagtttgtc atccatgaaa atgaggttta
    34921 tgtgatcgaa gtcaatcctc gagcaagtcg gactgttcca ttcttaagta aggtgacagg
    34981 aattccaatg gcacaaattg cgacgaaagc tattttggga gagaaactaa agacacttgg
    35041 ttatgaaaac ggactttatc cagaaacaaa agaagttcat gttaaagcac ctgtcttttc
    35101 ttttacgaaa ttgcagaaag ttgatactta cttagggcct gaaatgaaat cgactggaga
    35161 agtaatggga tctgacagac aattagacaa agctttgtac aaggcattcg aagcttctgg
    35221 attacacttg cctgaatatg gagccgtatt atttacgatt gcggacgaaa caaaagaaga
    35281 agcactagaa ttagctaaac gttttacaga aattggctat agtctgatag gaacgaaaca
    35341 tacagctgct tatttcgaaa aaaatggtct ggtcgttgaa acagtagcga aaatttctga
    35401 ggaagcagca gaaaaaaatg tagtcgactt aataagagat ggtaaaacac aagtggttgt
    35461 caatacgatt gataaagatc ggggaaatgc ttctaaagac ggtttcatca tccgaagaga
    35521 agcagttgaa catggtattc cattgtttac ttctttagac acagcagatg cgatcgttcg
    35581 agtgatggaa tcccgtgctt tttcaactaa ccctatttaa cggcgagcac atgttaagac
    35641 aaaaagacga aaaaccgttg caggagggga cagatgaaac aagaaatgat ggaaatcgtt
    35701 cagcaaaaac agctggcacc aaagattttt gaaatgacat tgaaaggtga tctagtaaaa
    35761 gaaatgcagc aaccaggaca gttcttgcat attctcgtgc cgcgtgcaga cttgctatta
    35821 cgccgaccga ttagcttgaa tcatatcgac cacgaaacaa atacttgccg tatcatttat
    35881 cgggtggaag gtgaggggac tagagtcttc tctactcttt ctgccggaga ccatttagat
    35941 gtcatgggtc ctttaggcaa tggttttgat ttgtccaatc ttcatacagg agacgaagta
    36001 tttataatcg gtggaggaat cggtgtccct cctctatatg agttatcaaa acagttagtc
    36061 gaaaaaggaa ttaagccaac gcatttttta ggttttgcca cacatgaagt cgtttattat
    36121 gaaaaagaat ttaatcgtct aggagaaaca agaatcgcta ctgacgatgg aacatacggt
    36181 atgcacggaa atgtcggtaa tttattgtta ggaacgaaga cggaacctac agctgttttt
    36241 gcatgtggtt caaacgggtt attgaagaca gtggaacagc tgttttcttc gcatcaaaat
    36301 gtccagttat cattagaatc ccgcatggca tgtgggatag gggcttgcta tgcttgcgtt
    36361 tgtcatacgc cggaagatga aacaggaaca aaaagtgtga aagtttgtga cgaaggaccg
    36421 atttttaaaa taggagaggt aattatatga atgagaatcc tttagcggtc aaacttcctg
    36481 gcctagaatt aaaaaatcct atcatgccgg caagcggctg tttcggtttt ggcaaagaat
    36541 acggaaaata ctatgatttg aatcagcttg gatcgatcat ggtcaaggca acgactccaa
    36601 atgcccgctt tggcaatccc acgccaagag tggcggaaac accatctggc atgttgaatg
    36661 caattggttt gcaaaatcct ggtctagacg ttgttatgca taaacttctt ccagaattag
    36721 aggagtacaa tgagttaccc atcatcgcaa atgttgctgg tgcttgtgaa gaagactatg
    36781 tagaagtttg tagcaaaatt ggcgaagcac caaatgtgaa agcaatcgag ctgaacattt
    36841 cttgcccaaa tgtcaaacat ggcggaattg cttttggaac agattctgat gttgcttttc
    36901 aattaacaca agcagtaaag aaagtttctt ctgttcctgt ctatgtcaaa ttgtccccaa
    36961 atgtaacaga catcgttcca atcgctcagg caatcgaagc tggaggggca gatgggttta
    37021 cgatgatcaa tactcttctt ggtatgcgga ttgaccttaa gacaagaaaa ccgattttag
    37081 ccaatcaaac aggtggactc tccggaccag caatcaagcc tgtcgctatt cgtttgattc
    37141 accaagtagc aagtatatcc agccttccaa tcattgggat gggcggcgtg cagacagtgg
    37201 atgatgttct tgagatgttt atggctggag caagtgctgt agctataggg accgcgaatt
    37261 tcaccgatcc gtatatctgt cctaaactaa ttaaagagct ccctgtgcga atgagcgaac
    37321 taggcatcga atcgctggaa agattaatca aagaagtgag agaggaacgt gaacgatgaa
    37381 tcaactagac caacgaccaa ttattgcatt agatttttca actaggcaag aggtagaaga
    37441 ctttcttcga tttttcccga aagaagaaaa attgtttgtg aaaatcggaa tggaattgtt
    37501 ctaccaagaa ggacctgaaa tcgtacgtta tctaaaagag gctggtcata atgtattttt
    37561 agatctgaaa cttcacgata ttccgaatac ggtcgaaaaa gcaatgcggg gattggcaaa
    37621 gcttggtgtt gatctcacct gtgttcatgc cgctggcggt attcgtatga tggaggcggc
    37681 aatgcgagga ttagaagagg ggactccaga aggtggaaaa cgtccgctac ttctagctat
    37741 cactcagctt acttctacaa gtgaagaaga aatgcatgcc gatcaactaa tcgaagtttc
    37801 tttagaaaaa agtgtgatcc attatgctag ctgtgccaaa aaagcaggat tagatggtgt
    37861 ggtttcttct gcttgggaag tagaagcgat caaagaaatg gcaggagacg aatttgtttg
    37921 cttaactccg ggtataagac cagaaggaac agtcgcagga gatcagacaa gagtagtcac
    37981 accttctcag gcaaaaaaga tcggctcaac ttttatcgtt ataggtcgac caatcactca
    38041 gtcaactaat ccttataaag catatcaaac gattcaaaca gaatggagct aacctaaaat
    38101 gaatgttgaa caaagtatcg caaaagactt attagaaatt gaagcagtat ttttaaatcc
    38161 aagtaatcct tttacatggg caagtggaat caaaagccca atttactgtg ataaccgaat
    38221 cacgatgagc tatccaaaag tccgaaaaga gatcgcaaaa ggattagcaa gcaaaatcaa
    38281 agaagcttac cctgaagtac aagtgattgc tggaactgca acagcaggaa tccctcatgc
    38341 cgcatgggta gcggaaatac tggatttgcc aatggtctat attcgaagta aggcgaaaga
    38401 tcatgggaaa ggtaaccaaa tcgaaggacg tatatttgaa ggtcagaaga tggttgtgat
    38461 cgaggatttg atttctacag gcggaagtgt cttggaagca gctgaagcag caaaaagaga
    38521 aggtgcggat atccttggtg ttgcggctat ctttacgtat gagttgccaa aaggaaaagc
    38581 aaatttcgaa aaagccgaaa tccctcttat gacgttgacc aattattctg tattgattga
    38641 agcagcttta gaagatcgtt atatcgatga gcaagaatta actctgctaa aagaatggaa
    38701 aaaagatcct gaaaattggc agatttaagc gcgcttgatg aagatagaga aatccgattt
    38761 gtgtctccag aatcatcaat tggaatagca aaaatccaaa aactgattgc gagaagcatc
    38821 ttttcatcag gtatgaaaag aa
//
LOCUS       DAXWLG010000007        36934 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXWLG010000007 DAXWLG010000000
VERSION     DAXWLG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.007"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            107..1279
                     /locus_tag="ACGE4T_000265"
     CDS             107..1279
                     /locus_tag="ACGE4T_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFF8385583.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            1544..2455
                     /locus_tag="ACGE4T_000266"
     CDS             1544..2455
                     /locus_tag="ACGE4T_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8385584.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(2711..3628)
                     /gene="rnhC"
                     /locus_tag="ACGE4T_000267"
     CDS             complement(2711..3628)
                     /gene="rnhC"
                     /locus_tag="ACGE4T_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFF8385585.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            3830..4267
                     /gene="zapA"
                     /locus_tag="ACGE4T_000268"
     CDS             3830..4267
                     /gene="zapA"
                     /locus_tag="ACGE4T_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFF8385586.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            4325..4872
                     /locus_tag="ACGE4T_000269"
                     /pseudo
     CDS             4325..4872
                     /locus_tag="ACGE4T_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            4987..7347
                     /locus_tag="ACGE4T_000270"
     CDS             4987..7347
                     /locus_tag="ACGE4T_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFF8385587.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            7434..7748
                     /gene="trxA"
                     /locus_tag="ACGE4T_000271"
     CDS             7434..7748
                     /gene="trxA"
                     /locus_tag="ACGE4T_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFF8385588.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            7930..9708
                     /gene="uvrC"
                     /locus_tag="ACGE4T_000272"
     CDS             7930..9708
                     /gene="uvrC"
                     /locus_tag="ACGE4T_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFF8385589.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            9853..10752
                     /locus_tag="ACGE4T_000273"
     CDS             9853..10752
                     /locus_tag="ACGE4T_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385590.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            10745..12007
                     /locus_tag="ACGE4T_000274"
     CDS             10745..12007
                     /locus_tag="ACGE4T_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8385591.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            12170..14068
                     /locus_tag="ACGE4T_000275"
     CDS             12170..14068
                     /locus_tag="ACGE4T_000275"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFF8385592.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(14367..15086)
                     /locus_tag="ACGE4T_000276"
     CDS             complement(14367..15086)
                     /locus_tag="ACGE4T_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFF8385593.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    15276..15323
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            15423..15755
                     /locus_tag="ACGE4T_000277"
     CDS             15423..15755
                     /locus_tag="ACGE4T_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385594.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            15742..17745
                     /locus_tag="ACGE4T_000278"
     CDS             15742..17745
                     /locus_tag="ACGE4T_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFF8385595.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            17764..18234
                     /locus_tag="ACGE4T_000279"
     CDS             17764..18234
                     /locus_tag="ACGE4T_000279"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFF8385596.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            18255..18839
                     /locus_tag="ACGE4T_000280"
     CDS             18255..18839
                     /locus_tag="ACGE4T_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFF8385597.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            18854..19840
                     /locus_tag="ACGE4T_000281"
     CDS             18854..19840
                     /locus_tag="ACGE4T_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFF8385598.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            19830..20141
                     /locus_tag="ACGE4T_000282"
     CDS             19830..20141
                     /locus_tag="ACGE4T_000282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFF8385599.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            20163..21944
                     /locus_tag="ACGE4T_000283"
     CDS             20163..21944
                     /locus_tag="ACGE4T_000283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFF8385600.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            21937..23313
                     /locus_tag="ACGE4T_000284"
     CDS             21937..23313
                     /locus_tag="ACGE4T_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFF8385601.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            23320..23955
                     /locus_tag="ACGE4T_000285"
     CDS             23320..23955
                     /locus_tag="ACGE4T_000285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFF8385602.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            23985..25340
                     /locus_tag="ACGE4T_000286"
     CDS             23985..25340
                     /locus_tag="ACGE4T_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8385603.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(25532..26926)
                     /locus_tag="ACGE4T_000287"
     CDS             complement(25532..26926)
                     /locus_tag="ACGE4T_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8385604.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            27018..27989
                     /locus_tag="ACGE4T_000288"
     CDS             27018..27989
                     /locus_tag="ACGE4T_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8385605.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            27986..28768
                     /locus_tag="ACGE4T_000289"
     CDS             27986..28768
                     /locus_tag="ACGE4T_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFF8385606.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(28937..29308)
                     /locus_tag="ACGE4T_000290"
     CDS             complement(28937..29308)
                     /locus_tag="ACGE4T_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8385607.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            29442..29885
                     /locus_tag="ACGE4T_000291"
     CDS             29442..29885
                     /locus_tag="ACGE4T_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8385608.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(29928..30737)
                     /gene="yidA"
                     /locus_tag="ACGE4T_000292"
     CDS             complement(29928..30737)
                     /gene="yidA"
                     /locus_tag="ACGE4T_000292"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFF8385609.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(30756..32123)
                     /locus_tag="ACGE4T_000293"
     CDS             complement(30756..32123)
                     /locus_tag="ACGE4T_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8385610.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(32107..32937)
                     /locus_tag="ACGE4T_000294"
     CDS             complement(32107..32937)
                     /locus_tag="ACGE4T_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFF8385611.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            33100..33537
                     /locus_tag="ACGE4T_000295"
     CDS             33100..33537
                     /locus_tag="ACGE4T_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFF8385612.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            33613..34233
                     /gene="rpoE"
                     /locus_tag="ACGE4T_000296"
     CDS             33613..34233
                     /gene="rpoE"
                     /locus_tag="ACGE4T_000296"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFF8385613.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            34422..34928
                     /locus_tag="ACGE4T_000297"
     CDS             34422..34928
                     /locus_tag="ACGE4T_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFF8385614.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            34945..35844
                     /locus_tag="ACGE4T_000298"
     CDS             34945..35844
                     /locus_tag="ACGE4T_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFF8385615.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            35931..36815
                     /locus_tag="ACGE4T_000299"
     CDS             35931..36815
                     /locus_tag="ACGE4T_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8385616.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        12142 a         6398 c         7875 g        10519 t
ORIGIN      
        1 aaaaaggata aagtccatat aattgtgtaa aagataaaag gccatgtaac agccctttta
       61 cggtacaatg tttttaacca caaaaacata cccaggagga cgttacatga cccaagtaca
      121 ttttacactg aacaacgaag aggttcaaag tattattgaa cattcggtaa aagatgatgt
      181 ttctaaaaat attttaacca ctgttttcaa ccaattgatg gaaaatcaac gaacagaata
      241 tattcaagcc gatgactatg aacgttcaga aagtcgtcag agtcaaagaa atggctatta
      301 tgagcgagac tttacgactc gtgtgggtac actcgaatta aaagtgccta gaacacgtga
      361 tggtgaattt tcaccgacgg tgtttgagcg ttatcagcga aatgaaaagg cactgctcgc
      421 ttcaatgctt gagatgtatg tttcgggcgt ttcgacacgt aaagtttcaa agattgttga
      481 agagctatgt ggaaaatctg tatcgaaatc ttttgtttct agcctgactg agcagttaga
      541 cccgatggtc aacgaatggc agaaccgttc actctcaggt acgaattatc cttatctgat
      601 gactgatgtt ctctacataa aagtccgtga ggaccatcga gtgctttcta aaagctgcca
      661 tattgcgatc gggataacag aaggtggcga ccgtgaaatc attggcttca tgattcaaaa
      721 tgaagaaagt gatgacacat ggtccatctt ctttgaatac ttaaaagaac gcggcctaaa
      781 ggggacagaa ctcatcattt ctgatgccca taaaggccta gtgtctgcga ttcgtaagtc
      841 atttaccaac gcaagttggc agagatgcca ggtccatttt ttaagaaaca tcttcagttc
      901 cattccaaaa aagaattcaa aaccgtttag agaagcagta aaagcgatct ttaagtttac
      961 ggatattgaa ctcgctcgaa cagctaagaa tgccttagtc ggtgaatata tcgaccagcc
     1021 taaatataca aaagcctgcg aaatattgga taatggcttc gaagatgcct ttcaatacac
     1081 ggttatcgga aatagtcata atcggctaaa aagcaccaac cttcttgaac gactgaacca
     1141 ggaagtccgc agaagagaaa agattattcg gatttttccc aaccgaacgt ctgccaatcg
     1201 attaattgga gctgtcctta tggaccttca tgacgaatgg ctcagttcta caagaaaata
     1261 tattaagttt gatcaatgag agaccggtaa aacattgtat agtattttac acaggattat
     1321 ggacttgact taattgtttg tagaatgaag gaaactattt attgggtaga tgaatcacca
     1381 cgaatagtca aaaggagatt tcaggatatg ccaaaaatga aaaaaatcat tttagcagta
     1441 atgttatttt ctacggttat atcttttaca gccccttttt tgcgtactta agctgatttt
     1501 aagttttgac ccttagtctg gatctacgag gtgagagtca agaatgaaaa aaataattgg
     1561 agcttttgta tttgcaatca ttatcattat tatctgtttt caaatagatg tttttggtca
     1621 aaaaaagata gttggacaaa cggtcagcag tacgaacagt acggttacaa agacaaagga
     1681 atcatttgat gatacagaac aagagacatt aatcaaaatc ggaaaaaatg accgtacact
     1741 atatacagat gactcgctaa ctaccccact tgcaactata aacggcggag agctggcgga
     1801 gtttctttct gaaacaaaaa atgcttatca gatcaagaca aatgatggct atactggata
     1861 tttagccctc gaagatggaa ccaaagtcac aaagaatata caaacaaaac cggaagagct
     1921 atctgatgca gtgattgtat tagatcctgg gcatggcgga aatgatactg gggcattaag
     1981 caatgatgaa caaacagaag aaaaagatat aacattaagt acagccataa aagtaaaaaa
     2041 ggcgctagag gatgctggag caacggttta tttgacccat actacagatg aattagtaca
     2101 gcttggagat atatgcgact acagtgaaga aaaaaaggca gatgtcttta tcagtttaca
     2161 cgcagactct acggaatatg cgaatgaagc aactggaatc acgacatatt attactatgg
     2221 acaagaggaa acattagcac agacaattgc tgactccttc actgatctgc ctctggattc
     2281 aagaggaatc tccactggga actatcaagt tcttcgagaa aatcttcaac cttctatttt
     2341 aatcgagatg ggttatatga ataatgatag tgatttaaag gaattagtaa cagacagtta
     2401 tcagcagcag attgctgcaa gtttgacaca agcattgacc acttattttc agtaataaat
     2461 aagcccagcc aaacaaaaat agctcctcat atttttcgtt tggccgggct tattatcgca
     2521 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactccgcg
     2581 tcacaaggag caatttcaaa aaacagcttc tcatgtttga tgactattgt gacttgtgac
     2641 cgaggagttg gcatgagaac accgtttatt cggaacaagg gactgtgaca tgacttttgt
     2701 cacagttcca ttattttttt agcagcttca aagctttttc tgtattggca aaatgaagtt
     2761 tggcatattt ttcaagcata ggcatgccgc ctttctccaa tagttttgcc gctacttggt
     2821 cagatttcgt tccagctcct gatggaaggg tgattccagc ttcagctgat gctttgtgta
     2881 actcttccaa aaagctggca cgactgatga tagaagcagc agcaacagct aaatgatatt
     2941 gttctccttt agtaacgaag tacagttttt cttgtaattg atttttttct aaacgcgcat
     3001 attttttaaa atttgcttcc ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg
     3061 gctcgatttt ttttagcagc aaatggatcg cttgattgtg caaagcgact ttcatccgaa
     3121 cggcattgta ttttggttgg atctcattgt atttttctgg agataagaca agcagtttat
     3181 aggggatggt tttttttaag atgtcagcaa gctggatgat ttggctatct tttaactctt
     3241 ttgaatctct aacaccaagt ttacgtaatt ctgcaagctg atcacgtttc acataagcag
     3301 cacatactgt tagcggacca aaatagctgc catttccgac ctcatcacta cctaaaacgg
     3361 ataattgact gatatttttt ggcaaagaag aggctgtttt tgctggcttg gcttgtttat
     3421 cctgtgcctc cccccattta tcagcttcta ttgctgcatt cgtaccttga aacatcactt
     3481 ttccggatgt ataagcggta atggtcgtcc cgtctttttt tgcagcaaag ctagtataag
     3541 gaatctgttt atttaacaaa taagagcgat agtatgcttt catttgacta agttggtcgt
     3601 ttgttgcttt gataatgacg ttgctcataa aattctcctc gtttttatac ataaaactat
     3661 accgcaactc acgacttttc gttttctaag agattgcgag acatagcatt tttttaaagt
     3721 ttatcatata atagttaagt atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact
     3781 tcatggtaca ctaaataaaa ctttcatcaa ataggaggaa atgattcgta tggcgcacga
     3841 aaaaacacgc tacaaagcag tgattgccaa ccaaacatat actattatcg gacgggaaac
     3901 caagcatcat atggatatcg taacgaagct gatcaatgaa cagttagcag aattgaaaca
     3961 attatcacca caaatggata atgaacaagc agcaatcttg atggctgtca atgcgctttc
     4021 ggatcaattg aagaaacaag aaagaatctt ggagctggaa gaagaaacag cagaattaaa
     4081 gaaaaaaatg atcaaattta cagagcttga aaatcgtgtg aaacgcattg aagcaatcga
     4141 gaacgaagct cgggaagtat tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca
     4201 tgtggaagca caacagattt taaacgagaa acgaaaagga caaattaaac aaaaatcttc
     4261 taattaagat ccttttatca aaataagaaa aatttaaaaa agttagaaga gaaaggaacg
     4321 tagcatgctg agtttgctga ttttattttt attgctaatt gcgttttttt ctggagcacg
     4381 tcgcgggttt gctttgcaaa tcatattcgc gattggatat gtattgtcat ttattgctgc
     4441 ccagcatttt tacaaaccat tagcaagtca tttggaatta tacattccat acccagctgt
     4501 caccccaaca tcaaagttag cattttttga tcaggtattt gcttttcacc tagatgaagc
     4561 tttttatgca ggaactgctt ttctgattat tttgctaatt ggctggctgc ttacccgatt
     4621 tgtgggggtt tttgtccatg gattgacgta tgtgccgatc ttgagacagg tagactggat
     4681 tgcaggaggc atcctgtcac taatcatggc ttacgttact atatttttga ttttgcagtt
     4741 acttgccttt gttccattag atgtggtcca aaatcaattc ggcgagcata gtttcgctcg
     4801 ttttatcgtt gagagaacgc catttcttac caacaagatg tatgagcttt ggtaacgaat
     4861 attttgaatt aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagtttat
     4921 ttaagaaggc tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt
     4981 gataaaatga ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc
     5041 agacaatttg tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagt
     5101 gagaaacaaa cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt
     5161 ctgcgaggcg ggatccctat acctaaactt gaaaatatcc gtccccatat gaaacggatc
     5221 gagatcggag cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg
     5281 acaagcgaat tgaatcgttt cattgatgat ctttctgaca gtgaaatcga atttgcacgt
     5341 ttgtatatgt gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa
     5401 gcaatcgatg aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga
     5461 cagaacattc ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg
     5521 aaaaatgcaa agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc
     5581 ccagtcaaac aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca
     5641 ggacagactt tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa
     5701 taccaaatcg ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt
     5761 gttccccatc gtcaagaaat catccacaat gcttatgtga ttggtaaaat ggatctgatg
     5821 aatgcaaaag cacgatttgg taaagaagta aaagccatcg tgccgggaat cagtgaagac
     5881 aatcatgtcg tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct
     5941 aatgacatca caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt
     6001 gggaaaacga tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta
     6061 ccgattcctg caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata
     6121 ggagacgaac agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact
     6181 gtagatattt tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca
     6241 ggaacagatc cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa
     6301 aagtctgctt atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatacaat
     6361 cgggcaaata cgattaatgc cagcatggag ttcgatgtgg atacactaag tccaacgtat
     6421 cgtttgctga ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt
     6481 cttgatacag aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta
     6541 aacgaaatga tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa
     6601 atgcgtcatt ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc
     6661 tatttctttg aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaacgaagtc
     6721 gtgtcagagg cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag
     6781 cagattgggc aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca
     6841 aatctacatc aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa
     6901 aaaacgttga aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt
     6961 ttacgtaaaa acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc
     7021 gaagacgagc tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct
     7081 gtacgatctg aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac
     7141 gaagaagcgc taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct
     7201 caagtgacta ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat
     7261 cttaaaaacc atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat
     7321 ggtgcgacaa ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc
     7381 caagtataga aatgctatac taatcataga ttttaaaaat ggaggtcttt gatatgtcac
     7441 aagtaattac agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt
     7501 tttgggcaac ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac
     7561 aagaatatga tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa
     7621 ctcctcaaca attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag
     7681 tagtagaaaa agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat
     7741 atttataaaa acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc
     7801 ggtttttttg cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt
     7861 gtcaggctgg attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg
     7921 gagtaccaga tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc
     7981 tgttacttga tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta
     8041 aaaaatcgtg tccgctcata ttttactggc agccataata cgaaaacgga acggcttgtt
     8101 agtgaaatcg aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta
     8161 gagatcaatc tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa
     8221 acctatcctt tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaaa
     8281 gtactaaaag acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag
     8341 acaaagaaga tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag
     8401 gagccttgtc tttactatca tttgggacaa tgtctgtgtc cttattattt cgacgtagat
     8461 ccgaaagtct atacgaaaat cgttgatgaa gtgaaaaagt ttttaaacgg aggacacgaa
     8521 aaaatcgagt cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa
     8581 aaagctgcag aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa
     8641 atgacaaata ctgatttact ggatcgagat gtatttggtt atgcgattga caaaggatgg
     8701 atgtgtgtcc aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg
     8761 tttcctttct ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag
     8821 gaaaatgagc atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt
     8881 gtagaagctt tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta
     8941 gttgaattgg cgaataagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc
     9001 agaaaacagg aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg
     9061 accccaatac gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct
     9121 gcgatggtcg tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata
     9181 aaaacagtca acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat
     9241 tcacgagtga ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa
     9301 ggacaagtag atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt
     9361 gctggcatgg caaaaaacga taaacataaa acaaatgagt tgctattcgg tatagatttg
     9421 gctgtagtac cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat
     9481 gaagtccatc gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct
     9541 tcccgattag atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag
     9601 ttcaaatcgt tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg
     9661 ccgaaaaatg tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact
     9721 ttacaaagaa agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt
     9781 gttggtaaat aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga
     9841 aggagtgaag aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct
     9901 gtcgatcatg tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac
     9961 ggggctggga agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga
    10021 gaagtgttat ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt
    10081 ccggaggaac ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca
    10141 gaacttagag ggaaatcaag aaaagagatt gaaccaaaaa tcgatttttg gatggaaaaa
    10201 tttcaggtaa aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa
    10261 aaagtccagc tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca
    10321 ttcagcgggt tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa
    10381 aaacatggtt catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc
    10441 gatcatttga tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt
    10501 cgtgaatcat ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta
    10561 gaagaaatcg aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc
    10621 ttagaagatc ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt
    10681 cctatgttta accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa
    10741 atcgatgcgt aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat
    10801 ttctttcttg atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg
    10861 aaactttgct caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca
    10921 aatcagtcag caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc
    10981 tgaagaaaaa gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc
    11041 aactgagaat gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac
    11101 acaactactg atacaacaac aactgactgg cttgcaatcg atgcttcgag caagtcaatt
    11161 aggcatttca ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa
    11221 agtcaatttt gattcaaaag gaaaaatgac agttggagaa gataactcga cagtccaata
    11281 tgtcgtaagt tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat
    11341 cattgcacaa gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag
    11401 tacgactgca caaaagcatt tttacggaaa actaacaggt gttttacttg ttgcattaac
    11461 gcaaatggca ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga
    11521 aatggtcaaa aatatattag acggtatctc attggataac ttgattggac cattcttgtg
    11581 gtattctctt ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg
    11641 tggctctttg gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct
    11701 atctctaggc ggatatatgc ttggattgat actaggagct tcagatccaa acaatatcgt
    11761 tattcggatc acttcgtata ttccattctt atcttcatat attatgccgg ttcgcttagc
    11821 aaatgagaca gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac
    11881 attcttattg atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tgtatagcga
    11941 aggcggatta tggagttcat tgaaacaatc gatttcaatt atgagaaatg aacgaaaaaa
    12001 agcataaaat atagaaaaat atagaaggaa gctggacatt tattgttccc agcttctttt
    12061 ttgtatagac ctatttctat ttttttatta tattggatat atatattcat tgaaaataga
    12121 gcataacaac tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta
    12181 tttagattta ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa
    12241 tctcgaagca attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg
    12301 cgaatttcct gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat
    12361 caaagacctt ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac
    12421 ggcttatcca gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct
    12481 gatccatcgt ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt
    12541 tcgaaaagga ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga
    12601 tcgtatcaac gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa
    12661 ggaagaagaa aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca
    12721 tttgcatatc gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg
    12781 aatcatggat ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg
    12841 tgcgtattgc ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa
    12901 ctatttattt gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc
    12961 ccatcaaacc tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga
    13021 aagagagcaa gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc
    13081 agaacatcag ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga
    13141 aaaaatcaac tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg
    13201 gacttgtttt caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa
    13261 agtgatcgat agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac
    13321 gtttttgatc gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg
    13381 ctgtatacct gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg
    13441 cggaaaagaa ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac
    13501 tgcaaatgaa gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc
    13561 tttattcggc aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac
    13621 gcacgtcgaa aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa
    13681 aattttggca gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc
    13741 agtaaaagta aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga
    13801 cggaggtcta tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg
    13861 caattcttac ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt
    13921 tgcaaaaaaa tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa
    13981 acttaatcgg aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag
    14041 gctgttggat tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact
    14101 aaaaagcgac tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg
    14161 taaaatatta gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt
    14221 tcacgacctc ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    14281 ccaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca
    14341 ggctcacgac ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc
    14401 tgttcagatg aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg
    14461 tgcatattat ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct
    14521 tttaacaaag gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa
    14581 tcagggagaa ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac
    14641 tgcggggcag tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct
    14701 tgataaagtg ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa
    14761 atcggttttc tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga
    14821 atatttgttt ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc
    14881 tgaccgcctg caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga
    14941 gacatgactg gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc
    15001 tgcggtgtat agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc
    15061 ccggcaatgc cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa
    15121 ggatttaaaa aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg
    15181 ttactatctg taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt
    15241 aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct
    15301 agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct
    15361 ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg
    15421 aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga
    15481 agcgtgctcg aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat
    15541 tggagataat ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac
    15601 aattcgaaaa gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg
    15661 tgcaagcaac tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca
    15721 ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca
    15781 gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc
    15841 cgggatctgt atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc
    15901 acgattattg ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa
    15961 gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa
    16021 cgtcaagaac tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat
    16081 aaattagaag aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat
    16141 cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt
    16201 atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa
    16261 agtttcaagg aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg
    16321 ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa
    16381 attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa
    16441 aaattgcaag aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg
    16501 acagcgttag gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca
    16561 cttgccgttg cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc
    16621 gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa
    16681 actgtatcag aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag
    16741 caggaagtcc ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact
    16801 gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt
    16861 tatcttgtct tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt
    16921 gggacaatac tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa
    16981 ttctttgaga ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc
    17041 ggacaggcat taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg
    17101 acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg
    17161 gttggattat tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc
    17221 agtgatggtt ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc
    17281 atgttgataa aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct
    17341 ttatgtatct taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa
    17401 ggagcgtata atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt
    17461 ttgatggcac taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct
    17521 ttcatgccgc cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca
    17581 ctgaatatgt ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc
    17641 gaattttttg gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg
    17701 gaaaaatacg tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat
    17761 acaatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg
    17821 gcaacagcga cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag
    17881 gcagcagcag cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa
    17941 ttgcttccag gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac
    18001 ttaggaaatg atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt
    18061 gcttttacag gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa
    18121 attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa
    18181 acatatgcga ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa
    18241 aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag
    18301 agcaagaaag agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac
    18361 aaaaacgtac gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac
    18421 ttgaaaaaaa ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt
    18481 tgaatgcgaa gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga
    18541 attgggatga atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga
    18601 caggcaaagt aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc
    18661 tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg
    18721 atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc
    18781 ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag
    18841 gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga
    18901 acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg
    18961 tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt
    19021 cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc
    19081 ggagcctgaa atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt
    19141 gacaaaagcc gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta
    19201 ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag
    19261 cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg
    19321 aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct
    19381 aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac
    19441 gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag
    19501 ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta
    19561 cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg
    19621 aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca
    19681 agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga
    19741 ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaatcaga
    19801 aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca
    19861 aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc
    19921 aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca
    19981 cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac
    20041 ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc
    20101 aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa
    20161 ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt
    20221 ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta
    20281 tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc
    20341 caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca
    20401 tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa
    20461 gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt
    20521 tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag
    20581 aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca
    20641 aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa
    20701 atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca
    20761 aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct
    20821 tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag
    20881 tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg
    20941 gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta
    21001 atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag
    21061 tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca
    21121 tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg
    21181 agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt
    21241 cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc
    21301 gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac
    21361 cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag
    21421 cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag
    21481 aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg
    21541 gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg
    21601 attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt
    21661 atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca
    21721 aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg
    21781 cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca
    21841 aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa
    21901 cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc
    21961 ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa
    22021 ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa
    22081 gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct
    22141 tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga
    22201 gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat
    22261 ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc
    22321 caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca
    22381 gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca
    22441 acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt
    22501 gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc
    22561 atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct
    22621 ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca
    22681 gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca
    22741 ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca
    22801 ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa
    22861 gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc
    22921 ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt
    22981 tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg
    23041 atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt
    23101 ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt
    23161 gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat
    23221 ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac
    23281 atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat
    23341 ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat
    23401 aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag
    23461 aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta
    23521 ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa
    23581 aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat
    23641 tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt
    23701 gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg
    23761 acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga
    23821 gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt
    23881 atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg
    23941 ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca
    24001 aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga
    24061 ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg catgggacaa
    24121 attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat
    24181 tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga
    24241 tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag
    24301 tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg
    24361 tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg attggtacgg
    24421 ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt
    24481 tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag
    24541 ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg
    24601 ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga
    24661 ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt
    24721 tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg
    24781 caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc
    24841 tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat
    24901 ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc
    24961 gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg
    25021 cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca
    25081 tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg
    25141 aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg
    25201 caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg
    25261 tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag
    25321 aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa
    25381 gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt
    25441 tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca
    25501 ataagatttg attcctttat ttatcaatag gttataattc atattttttt attttttctt
    25561 ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa
    25621 cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt
    25681 tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc
    25741 gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat
    25801 tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga
    25861 ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg
    25921 cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag
    25981 aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga
    26041 aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa
    26101 taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga
    26161 tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt
    26221 ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa
    26281 cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg
    26341 ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa
    26401 caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat
    26461 ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa
    26521 caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt
    26581 caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg
    26641 tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca
    26701 aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat
    26761 aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa
    26821 tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt
    26881 ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt
    26941 aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt taatcttttg
    27001 tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt
    27061 ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca
    27121 gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga
    27181 tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc
    27241 cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc
    27301 aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg
    27361 ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa
    27421 ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca
    27481 ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta
    27541 aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc
    27601 cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg
    27661 taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa
    27721 gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag
    27781 aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg
    27841 gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga
    27901 cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata
    27961 acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc
    28021 agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga
    28081 aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa
    28141 gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg
    28201 tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac
    28261 gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta
    28321 catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc
    28381 gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga
    28441 tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg
    28501 aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt
    28561 tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc
    28621 ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg
    28681 cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt
    28741 gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg
    28801 gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt
    28861 aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta
    28921 aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga
    28981 caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccag
    29041 cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat
    29101 agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt
    29161 catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc
    29221 ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca
    29281 ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc
    29341 ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt
    29401 aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg
    29461 gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa
    29521 gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg
    29581 aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg
    29641 gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt
    29701 atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta
    29761 taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg
    29821 tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa
    29881 actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat
    29941 tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct
    30001 tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg
    30061 gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc
    30121 tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa
    30181 tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt
    30241 gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt
    30301 gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc
    30361 gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga
    30421 tgactgccga cacggcgtgc cattacttcg atctctagga aatcttcatg tgtcagtccg
    30481 tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata
    30541 acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag
    30601 ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct
    30661 tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg
    30721 actagtttga tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca
    30781 ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc
    30841 aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc
    30901 gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc
    30961 aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga
    31021 tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac
    31081 aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt
    31141 ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac
    31201 cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta
    31261 aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct
    31321 tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg
    31381 gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc
    31441 actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt
    31501 aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc
    31561 aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg
    31621 gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta
    31681 agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga
    31741 ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac
    31801 agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt
    31861 tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct
    31921 aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat
    31981 tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga
    32041 tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt
    32101 tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct
    32161 ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc
    32221 ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc
    32281 ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac
    32341 tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat
    32401 gacttgccca cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc
    32461 atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata
    32521 agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc
    32581 gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc
    32641 actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc
    32701 acctgaattt ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga tcccgtcact
    32761 tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa
    32821 atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa
    32881 aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca
    32941 accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt
    33001 gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact
    33061 aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca
    33121 ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat
    33181 tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag
    33241 attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat
    33301 gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg
    33361 ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa
    33421 ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga ttatgatctg
    33481 ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata
    33541 taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag
    33601 gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg
    33661 atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta
    33721 gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca
    33781 caattttata ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg
    33841 ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac
    33901 gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat
    33961 gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa
    34021 gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat
    34081 aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca
    34141 ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa
    34201 gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga
    34261 cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt
    34321 tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc
    34381 accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata
    34441 agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt
    34501 atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg
    34561 tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc
    34621 attttagtgc atggttttat cggatattga tccgcacggc attagatagt tggcgcagac
    34681 aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag
    34741 aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga
    34801 ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata
    34861 tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc
    34921 tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag
    34981 aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt
    35041 attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc
    35101 ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt
    35161 cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa
    35221 cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat
    35281 gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca
    35341 gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg
    35401 ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc
    35461 caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt
    35521 gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa
    35581 gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact
    35641 gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt
    35701 ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct
    35761 ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca
    35821 tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca
    35881 agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa
    35941 attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg
    36001 tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat
    36061 ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca
    36121 aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag
    36181 acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg
    36241 gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg
    36301 ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag
    36361 ctgaagcagg gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt
    36421 ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg
    36481 gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga
    36541 tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc
    36601 ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc
    36661 atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc
    36721 aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact
    36781 attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata
    36841 acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc
    36901 tatacaggta caacaaaaaa ctgtccacca attt
//
LOCUS       DAXWLG010000008        36790 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXWLG010000008 DAXWLG010000000
VERSION     DAXWLG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.008"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..323
                     /locus_tag="ACGE4T_000300"
     CDS             <1..323
                     /locus_tag="ACGE4T_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8385617.1"
                     /translation="VNEEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKD
                     GVPYLHCHHIEYLSQGGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSF
                     SKIP"
     gene            666..989
                     /gene="relB"
                     /locus_tag="ACGE4T_000301"
     CDS             666..989
                     /gene="relB"
                     /locus_tag="ACGE4T_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8385618.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            1309..1536
                     /locus_tag="ACGE4T_000302"
     CDS             1309..1536
                     /locus_tag="ACGE4T_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFF8385619.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1552..2718
                     /gene="hemW"
                     /locus_tag="ACGE4T_000303"
     CDS             1552..2718
                     /gene="hemW"
                     /locus_tag="ACGE4T_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFF8385620.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2953..3996
                     /gene="hrcA"
                     /locus_tag="ACGE4T_000304"
     CDS             2953..3996
                     /gene="hrcA"
                     /locus_tag="ACGE4T_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFF8385621.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4090..4653
                     /gene="grpE"
                     /locus_tag="ACGE4T_000305"
     CDS             4090..4653
                     /gene="grpE"
                     /locus_tag="ACGE4T_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFF8385622.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4704..6533
                     /gene="dnaK"
                     /locus_tag="ACGE4T_000306"
     CDS             4704..6533
                     /gene="dnaK"
                     /locus_tag="ACGE4T_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFF8385623.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6684..7850
                     /gene="dnaJ"
                     /locus_tag="ACGE4T_000307"
     CDS             6684..7850
                     /gene="dnaJ"
                     /locus_tag="ACGE4T_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFF8385624.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8218..8955)
                     /locus_tag="ACGE4T_000308"
     CDS             complement(8218..8955)
                     /locus_tag="ACGE4T_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8385625.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(8957..10459)
                     /locus_tag="ACGE4T_000309"
     CDS             complement(8957..10459)
                     /locus_tag="ACGE4T_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8385626.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            10718..12712
                     /gene="uvrB"
                     /locus_tag="ACGE4T_000310"
     CDS             10718..12712
                     /gene="uvrB"
                     /locus_tag="ACGE4T_000310"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFF8385627.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            12722..15538
                     /gene="uvrA"
                     /locus_tag="ACGE4T_000311"
     CDS             12722..15538
                     /gene="uvrA"
                     /locus_tag="ACGE4T_000311"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFF8385628.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            15801..17066
                     /locus_tag="ACGE4T_000312"
     CDS             15801..17066
                     /locus_tag="ACGE4T_000312"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFF8385629.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17070..17801
                     /locus_tag="ACGE4T_000313"
     CDS             17070..17801
                     /locus_tag="ACGE4T_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFF8385630.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            17936..18820
                     /gene="rapZ"
                     /locus_tag="ACGE4T_000314"
     CDS             17936..18820
                     /gene="rapZ"
                     /locus_tag="ACGE4T_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFF8385631.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            18817..19815
                     /gene="yvcK"
                     /locus_tag="ACGE4T_000315"
     CDS             18817..19815
                     /gene="yvcK"
                     /locus_tag="ACGE4T_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFF8385632.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            19849..20784
                     /gene="whiA"
                     /locus_tag="ACGE4T_000316"
     CDS             19849..20784
                     /gene="whiA"
                     /locus_tag="ACGE4T_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFF8385633.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            21352..22008
                     /locus_tag="ACGE4T_000317"
     CDS             21352..22008
                     /locus_tag="ACGE4T_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385634.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            22187..22752
                     /locus_tag="ACGE4T_000318"
                     /pseudo
     CDS             22187..22752
                     /locus_tag="ACGE4T_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23451..23972)
                     /locus_tag="ACGE4T_000319"
     CDS             complement(23451..23972)
                     /locus_tag="ACGE4T_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFF8385635.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            24170..27850
                     /gene="nifJ"
                     /locus_tag="ACGE4T_000320"
     CDS             24170..27850
                     /gene="nifJ"
                     /locus_tag="ACGE4T_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFF8385636.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            28039..28926
                     /gene="cdaA"
                     /locus_tag="ACGE4T_000321"
     CDS             28039..28926
                     /gene="cdaA"
                     /locus_tag="ACGE4T_000321"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFF8385637.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            28923..30092
                     /locus_tag="ACGE4T_000322"
     CDS             28923..30092
                     /locus_tag="ACGE4T_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFF8385638.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            30116..31471
                     /gene="glmM"
                     /locus_tag="ACGE4T_000323"
     CDS             30116..31471
                     /gene="glmM"
                     /locus_tag="ACGE4T_000323"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFF8385639.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(31543..33348)
                     /gene="glmS"
                     /locus_tag="ACGE4T_000324"
     CDS             complement(31543..33348)
                     /gene="glmS"
                     /locus_tag="ACGE4T_000324"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFF8385640.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            33758..34048
                     /locus_tag="ACGE4T_000325"
     CDS             33758..34048
                     /locus_tag="ACGE4T_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385641.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            34061..35086
                     /gene="sppA"
                     /locus_tag="ACGE4T_000326"
     CDS             34061..35086
                     /gene="sppA"
                     /locus_tag="ACGE4T_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFF8385642.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            35089..35748
                     /locus_tag="ACGE4T_000327"
     CDS             35089..35748
                     /locus_tag="ACGE4T_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8385643.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            35905..>36790
                     /locus_tag="ACGE4T_000328"
     CDS             35905..>36790
                     /locus_tag="ACGE4T_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385644.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDF"
BASE COUNT        12012 a         6370 c         8046 g        10362 t
ORIGIN      
        1 atgttaacga agaggagttt tgcttctcaa aaacaaaaat atataaacgt aatcccttcg
       61 tatctgaata tgttaaacga cttgcaaaag gtatttgtca actttgccaa gaaaaagggc
      121 catttataaa agacggtgta ccttatttac attgtcatca tatagaatat ttgtcgcaag
      181 gtggaaaaga tgtgattgaa aactgtatcg cgttatgtcc caattgtcat gctagaattc
      241 atgaacttga gttacctgac gataaagaaa aattgctgaa gatcgttgaa aaaagagaaa
      301 atagcttttc taaaataccg taatttattt ttataggaca aataagctaa tgtgagctat
      361 gccttctata taagactaag ttctattata agtaacgaag taattctcgt tccttcctgt
      421 agtaatattt tttaaataac ctataggctt cttcaaaagt acatcctcca gtaaattcta
      481 agtactggag gattcttttt ctagtgttgc tttactcatg aaaaagtagt tggctacttc
      541 atcaaaattt ctgaactttt ttaatgtata ataaagagct cgatcattta ctacaaggat
      601 gctagctcct acattttcac agttttttag caagtaaatt tttctaaaat cacatcagga
      661 aatgcatgag attctttaag agaggcaatt atagggctat gattacggtt cgtgtaagtg
      721 acgccaaaaa agaatggctt tcttatatgg ctgaatttta tgggattact ttatctgaac
      781 ttctaaaaaa ctattcgatg gaacaactag aagatgaata caatcgtcaa acggctgaaa
      841 ttgcccataa acattggata agaagtggga aaacaacagt attcatggaa gaatccttaa
      901 tgaatttggt gggctggaat gacctgccgt ttaaaattca cgcccgaaat tcagaagcag
      961 ctctgattgg caaatacgtg tattggtaga gatagaagat aatcaattcg ttgttatcgc
     1021 tattcaagta gggcaccgaa gaaatgtcta tgattaagag actgtttatt tttacaaaaa
     1081 aataataatt ggaatctagt taaaactctt gaagtgagag atttgactag atttttttat
     1141 gaataaaata aggacaaaat tgtttaggaa gaatcatgag tgtaaaaaaa tcatccaaga
     1201 agtcgattct tggatttttt agtggatttg ttcgccaaat acgtttagta tgcgtatatg
     1261 agaggttgta agcaaagcgt tgtggtctga gcacttaagg aggaatctgt gaaaatagct
     1321 tttcatattt ttgcagattt tgacttaatg ccgaaggtca gctttgcgat taccgtttat
     1381 cgagaggctg taaaagcacc gtgtagctcc gatgacaata agcaagcatt tttagagcta
     1441 ggtttccttg attctaaata tgcggcttat ttcaatgagt tggtgcttga acgcgatttt
     1501 aagaaggtcg aaaacaggca cgacttttcg acgtaaggag aaagaatttc aatgatagaa
     1561 acaataaatc ccgcagaaaa caaggtttct gcgtacattc atatcccatt ttgcgagcat
     1621 atctgctatt actgtgactt taataaagtt tttttagaag ggcagccagt agatgagtat
     1681 gtggaaatgc tgctgaatga aatgagatta atgctggaaa gtcatcctac agcgaatttg
     1741 gaaacacttt atgtaggagg cggaacacca acttctttat ctgctaaaca acttgatcga
     1801 ctattatcag gtgctagaga gatcctgcct tttcaaaatg gtaatgagtt tactgtagaa
     1861 gcgaatcctg gtgatttgac ccgtgaaaaa cttcaagtga tgaaaaacta tggcgtcaat
     1921 cgtttatcca tgggagtgca gacatttgac aatcgcttat tgaaaaaaat tggacgtaaa
     1981 catactgcag aagatgtgta tcaaacaatg caatttttag aagcagaaaa ttttaccaat
     2041 gtaagcatcg atttgattta tgcattaccg ggacaaacac tagaaggata tcgggatact
     2101 ttggaaaaag ctttggcttt ggatctgcct cactattctt tgtactcatt gatcctcgaa
     2161 aacaaaacaa tgttcatgaa ctgggtacga caaggaagac tcgaactgcc tgatcaggaa
     2221 acggaaacca gaatgtttga agagacgatt gaagcaatgg aaaagcatgg acgtcaccaa
     2281 tatgaaataa gtaactttgg gctctccgga catgaatctc aacataacct catgtactgg
     2341 aacaatgacc actattttgg gtttggagct ggtgctagcg gttatcttgg agatattcgt
     2401 taccgcaaca aaggaccaat ccaacactat ttacgcccat tacgagcaaa tgaacgcccg
     2461 gtattggaag aagaaaaatt gacacgaaaa aatcaaatag aagaagaaat gttcttagga
     2521 ctaagaaaaa aaacagggat tactaaaaaa catttctatg atcgctatca aaaaacaatt
     2581 gaatcggtgt acggtgatgt gctaagggaa ttagagcaag aagggttact tgttaatgaa
     2641 gaagaccgtg tttatttgac accaagaggg acttttttgg ggaacaatgt gtttgagcgc
     2701 tttttatttg aaaaatagtt tgaagaatat ttgtttttta tcgaaacttg aaaagaaaaa
     2761 gtttagaaag tttgacaatg aggtattgaa gtattgatat aactggattt acatcaatta
     2821 tttagaatta gcactcttgt gtttagagtg ctaaaaaatg gagtctttct cttgacagga
     2881 gaagggcttc ttgctatatt tataagtgaa ttagcactta gaatatatga gtgctaatga
     2941 gaggtgagga agatgttaac aaagcgacaa aacgacattt tacgtctgat catccaacac
     3001 tatacgagta gtggcgtgcc cgttggttcc aaaacattaa tgtctgaggg agtcaaggcc
     3061 agctcagcaa cgattcgcaa tgatatgaaa gcattagaag atgaaggatt gctgttgaag
     3121 actcactctt cttctgggcg gattccatct gctttaggct atcgttatta cgtggatcat
     3181 ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc tgattcgtca gtctctaggc
     3241 agagaatttc atgaaatcaa tgagattatt aagcaatctg ctgagatatt atctgagctg
     3301 acaagctata cgactttttc attgggacct gaaataaaag accgccgact gactggcttt
     3361 cgtatcgtac ccttgaataa tcgacagatt attgctatcg tcgtaacaga tatggggaat
     3421 gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa gtcaggaact agaaaaaatg
     3481 gtacagattg taaacgaccg tctagtcgga gatcctctga tgacggtgta ccataaactt
     3541 cgtacggaga ttccgatgat tcttcataaa tatttccaga cgacagaagg gatgtctcag
     3601 ttatttgaca ccgttcttgg tcatgttttt gaagaaaagg tttatgtcag tggacaaatg
     3661 aatctcttgg attatgagcc ccgtcaagat cttgatcagt tcaaatcgat gttttctttt
     3721 atgaaagact cagacggact tacacaaatg atcactccga tggatagcaa tattcatatc
     3781 aagattggtg ctgaattagg caatgattta ttgcagaata tgagtatgat tcaagcaagt
     3841 tacgagatag atggtcatgg aagaggtaca atagccttgt tagggcctac tagtatgcct
     3901 tattctcgga tgttcggtct agtcgatgtt tatcgaagag aactagcagc taaacttgct
     3961 gattactacc gctcgcttga cttgtcaaac tcgtaatgtg aataacgaag ctatttgcag
     4021 ctgatcgttc tctctttctt agtaagagca atcaaacagc tgactgaata atgaaaggaa
     4081 gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag aaatgacaga tgctcagcca
     4141 gaaccggaaa tcgatgtcga agctgcagaa gattcgggta tttcagaagc tgaagcggaa
     4201 gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa tggaagataa atacttgcgg
     4261 gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa atgagcgtga gcaattacaa
     4321 aaataccgtt cacaagattt agccaaaaaa ttacttccat caattgacaa cctggaacgt
     4381 gcattagcca cagaagtgtc agatgatcaa ggggcaggtt tgaaaaaagg cgtggaaatg
     4441 gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa ttgaaaaaat ccctgctaaa
     4501 ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa ctgttccagc gactgaagat
     4561 acaccagccg atacgattgt agaagtacta caagaaggct acaaattaca tgatcgcgtg
     4621 ttaagaccaa ctatggtcat tgttgcacaa taattgtgcg gatataaaac ttacactttc
     4681 aattaaagaa ggagacgtta attatgagta aaattatcgg tattgactta ggtacgacta
     4741 actctgcagt agcagtattg gaaggaaacg aagcaaaaat catcgctaac ccagaaggaa
     4801 accgtacaac accttctgtt gtatcattca aaaatggaga aatccaagta ggggaagttg
     4861 caaaacgtca agctgtgaca aacccaaata cgatttcatc aatcaaacgc catatcggtg
     4921 aagcaggtta caaagtagat gtagaaggta aatcatatac accacaagaa atctcagcaa
     4981 tgatccttca atatatcaaa ggattcgcag aagactattt aggtgaaaaa gtcgaaaaag
     5041 cagttatcac tgtgccagct tactttaatg atgcacaacg tcaagcaaca aaagatgctg
     5101 gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga accaactgca gctgctcttg
     5161 cctatggttt agataaaaca gatcgcgacg aaaaaatctt agtattcgac cttggtggcg
     5221 gtacttttga cgtatctatc cttgaactag gagacggtgt ctttgatgta ttgtcaacag
     5281 ctggcgacaa ccatctaggt ggggacgact ttgataacaa aatcatcgac tacatggtag
     5341 cagaattcaa aaaagaaaac agcattgact tgtctcaaga caaaatggct ttacaacgct
     5401 tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg cgtatcaagc acacaaatca
     5461 gcttgccatt tatcacagca ggagaatctg gaccattgca tttggaaatg acattgacac
     5521 gtgcaaaatt tgatgaatta acagctgact tagtagaacg tacaaaagta ccagtacgtc
     5581 aagcgttaaa agatgcaggt ctgtcacaat cagaaatcga cgaagttatt ttagtcggtg
     5641 gatctactcg tatccctgca gttgtagaag cagtacgtaa agaaacagga aaagaaccaa
     5701 acaaatcagt taaccctgat gaagtagttg ctatgggtgc tgctatccaa ggtggcgtga
     5761 tcactggtga tgtgaaagat gttgttttac ttgacgttac accattgtca ttaggtatcg
     5821 aaacaatggg tggagtgttc actaaattga ttgaccgcaa tacaacgatt ccaacaagta
     5881 aatcacaagt attctcaact gcggcagata atcaaccagc agtagacatc catgtattac
     5941 aaggtgaacg cccaatggct gctgacaaca aaactctagg aagattccaa ttgacagata
     6001 ttccagcagc tcctcgtggt attccacaaa tcgaagtaac attcgatatt gataaaaacg
     6061 ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa agaacaaaaa atcacgatca
     6121 aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat ggtgaaagat gcagaagcaa
     6181 acgctgaagc agataaagct cgtaaagagg aagttgacct acgtaatgat gtagacgcat
     6241 tgctattctc tgtggacaaa acattgaaag aattagaagg caaagtggac gaagaagaag
     6301 tgaaaaaagc agaaacagct cgtgacgaat taaaagccgc tgttgaagca aacaacatcg
     6361 aggaaatgaa aactaaacgc gatgcattga acgaaatcgt tcaaaacttg actgttaaac
     6421 tatacgaaca agctgcacaa caacaagctc aagaaaatcc agaagcagct caaggcggag
     6481 cagatgatgt cgttgatgct gattttgaag aagtcgatgg cgacgataaa taatcagttc
     6541 actgaatttc gagagaaggc caaagttgct actttggctt tttcttttga aaaatagttt
     6601 gaagtatcgg tttttgtttc aaacaaaata tgatatgata acaagcgacg tgaaaatgat
     6661 gagaaccaat aggaggaaag cctatggcaa caaaacgtga ttattatgaa gtcttagggc
     6721 tctcaaaagg agcatcagaa gatgaaataa agaaagctta tcgtaagctt tcgaaaaaat
     6781 accatcctga catcaataaa gaacctgatg cggaagaaaa attcaaagaa gtatcagaag
     6841 catatgagat tttaagtgac ccgcaaaaac gcgctgcata cgaccaatac gggcatgcag
     6901 gcacagatcc aaattacggt gctggtggag ctggtggcgg atttggtgga ttcggcggct
     6961 tctctggtgg cggcttcggt ggttttgaag atatcttcga ctctttcttt ggaggaggcg
     7021 gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga tttgcaatat acggtcgact
     7081 tgtcttttga agaagcgatt tttggtgtag aaaaagaaat caagtataat cgggaagaaa
     7141 tctgtcatac ttgtggtgga aacggtgcaa aaccaggcac acaaccaact acttgtcata
     7201 aatgtcatgg tgcaggtacg atcaatgtag aacgccaaac gccacttgga cgagtgatga
     7261 gccgtcaaac ttgtgatgtt tgtcatggca cagggaaaga aatcaaagaa ccttgtccaa
     7321 cctgtcatgg ctcaggacat gagaagaaag cacattcagt taaagtgaat gtaccagctg
     7381 gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg agaagctgga gaaaacggcg
     7441 gaccatttgg tgatttgtat gtggtttttc gtgtagaaga aagtgatatc tttgatcgtg
     7501 aaggttctga aatttactat gaattgcctt taagttttgt acaagcagct ttgggggatg
     7561 aagtccaagt accaactgtc catggagatg tgaaattgaa gatccctgct ggaacacaga
     7621 caggtacgaa cttccgttta cgtggaaaag gcgctccgaa gttacgtggt ggtgcaacag
     7681 gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa tctaaatgaa caacaaagag
     7741 aagcattacg tgcattcgct gaagctggcg gcatcaaagt tgaagaacag caagaagacg
     7801 gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa gcggaagtag tttttctagt
     7861 aaaaatataa agtagacttg cccaaatttt ctatttctag taaaaacgct aagtcattaa
     7921 taattaaaaa ccagacgaac aatcatacgt tcgcctggtt tttttgtagt tgtcatgttt
     7981 gtaccaacaa aaaagccagc tttagaataa gataatcatc atagtttctg aaggtggtat
     8041 cgactatgta cttgatatcg tcaatgaacc gatggtactt tcttatgcga tggaacaggt
     8101 agagcgattg cttgtttgtg caggagaaca agcgatgcgg ctggtacagt taagtgaaaa
     8161 aacaagaaac taaaaatgcc cggtcgtcat gaagcgaccg ggcatttttg gtttttatta
     8221 aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat tttcaaagat
     8281 ttgttgaggc gttccgtctt ctaaaaagtt tccgtcgtca atgaacatga cacgattggc
     8341 cacttcttta gcgaagccca tttcgtgcgt aacgataacc atcgtcatac cttgttctgc
     8401 taactttttc atgacgttca agacatcacc gaccatttcg gggtcgagtg cagatgtagg
     8461 ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag ccacacgctg
     8521 cttttgtcca ccggaaagca tttcaggata catatctttt ttatcttcta gaccaactgt
     8581 ctgcagcaat ttgattgctt ttttctctgc ttcttctcta gattctttct tcaggtccat
     8641 cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa agtgctggaa
     8701 taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca tcaagtgggc
     8761 gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc aacgcaaaaa
     8821 tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt cgttgatgga
     8881 cacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat gctcaactaa
     8941 gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat tttgtcaatg
     9001 ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta ctttgtgttg
     9061 tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt gcggaaatga
     9121 tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc atgatgcgta
     9181 tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct aaacttctgg
     9241 aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc tctgcgatat
     9301 aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga atcgtaaatc
     9361 caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt ggaattcctc
     9421 ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt gctacactga
     9481 ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa gttaggagaa
     9541 ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt ccaagaaatg
     9601 ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct acatattctt
     9661 taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca ttgaacatat
     9721 taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg ccagattctt
     9781 ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt accggataat
     9841 catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga tcagcagcat
     9901 caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat tcagcacttt
     9961 cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac ttgatcgaag
    10021 tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca gaaaaatcat
    10081 agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct gcttgtcctg
    10141 attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg ttaaatcctt
    10201 gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg ccctttgcgt
    10261 tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg tcttttttgg
    10321 gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg ttgtcagctt
    10381 gtgcaacatg tccaaacgca agtgtcacaa gtcctgtcat taagaaaaca aatgagtgaa
    10441 ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt attataggcg
    10501 aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg cgagtttcta
    10561 acattttatg ttataaaacg taacatgcat aataagataa aaaagtggcg aaagtatgtt
    10621 cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt ttattggtcg
    10681 taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag atacctcccg
    10741 acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag cgattaacca
    10801 attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg cgactggaac
    10861 agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa cactaattat
    10921 tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat ttttccctaa
    10981 caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag cttatgtgcc
    11041 ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg acaaattacg
    11101 acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg catccgtttc
    11161 atgtattttt ggattaggtt cgccttttga gtaccaaaaa caagtagttt cgattcgaca
    11221 gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc aattcgaaag
    11281 aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg tggagatttt
    11341 tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg aggtcgaacg
    11401 tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac acgtggcaat
    11461 cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg tagcgaatat
    11521 caaagcggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac ttctggaagc
    11581 tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa tgggctacac
    11641 gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg aaccgcctta
    11701 tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat cgcacgtaac
    11761 catgcctcaa gtcagaggaa tgtataatgg tgaccgagcg agaaaacaga tgctagtaga
    11821 ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag aagagtttga
    11881 aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg aacatgaaca
    11941 aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc cagtgatcga
    12001 agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg aacgagtcga
    12061 aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag acctcacaga
    12121 ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca aaacgctgga
    12181 acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag ttggaatcaa
    12241 tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct tggatgcgga
    12301 taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac gagctgctcg
    12361 gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta tgcgtctagc
    12421 gatggatgaa acatcaagac gccgtacgat tcagcagaag tacaatgaag aacatgggat
    12481 cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga aagaatcgga
    12541 agatgataca aaagaagcgg tacaagtctc gtatgaagaa atgacgaaag aagaaaaaga
    12601 tacgttattg atgaaattag aaaaagaaat gaaaaatgcg gcgaaagcat tggattttga
    12661 aacagccgct aatgtgcgag atatgatttt agaattaaaa gcttcaaagt aggaggacaa
    12721 catggctaac gataaaatta cgattcacgg tgcacgtgcc cataacttga aaaatataga
    12781 tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt ctggaaaaag
    12841 ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag agagcctttc
    12901 tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata gcatcgacgg
    12961 attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc ggtcgacagt
    13021 aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg taggacatcc
    13081 gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa tggcagatca
    13141 agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg tggtaaagaa
    13201 aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg tccgcgttcg
    13261 agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa acaaaaaaca
    13321 tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt ctcgtctatt
    13381 tgattcttta gaggcggctc tgcgattggc agacggctat gctctggttg acgtgatcgg
    13441 tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat ttactgttgg
    13501 cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc cagactgtga
    13561 tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata gaacgaagac
    13621 acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt atcctcaaat
    13681 gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg aagaattacc
    13741 ggaagagcat caggatatca tcttgaatgg gacagatgat cttttccatt tccattatga
    13801 aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat tgaagaatat
    13861 cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc gtctttatat
    13921 gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag ctctatctgt
    13981 tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa aagcagtaga
    14041 tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac cgattttaaa
    14101 agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc taactttgag
    14161 tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg ctacacaaat
    14221 tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg gattgcatca
    14281 acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg gcaacaccct
    14341 cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatctga ttgacgtcgg
    14401 acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg aagaagtagc
    14461 taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa tccctgtacc
    14521 aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt cagaaaacaa
    14581 tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca caggtgtgtc
    14641 tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag cgcaaaaatt
    14701 aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg aaagtatcga
    14761 aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta atccagcgac
    14821 ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg aggcaaaaat
    14881 gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt gtgaagcttg
    14941 tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct atgttccttg
    15001 tgaagtatgt catggcaaac gatataactc tgaaacatta gaagtgcatt acaaaggaaa
    15061 aagcattgct gatattttgg aaatgacagt agaagatgct gtagaattct tcaagcacat
    15121 tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt atgtgacgat
    15181 ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac ttgccagtga
    15241 attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa cgacaggact
    15301 tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag atgctggtaa
    15361 cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt atatcattga
    15421 tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa caccagaaga
    15481 aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa tggtataaag
    15541 aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc ttgttttatt
    15601 aatatcagtg ttctagaatc attacatggt agttctttct acactgattg gtatagacaa
    15661 aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg agtttccttt
    15721 gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa aaacaagaga
    15781 ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt atccctatat
    15841 tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa gcatacggca
    15901 aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt aaaattgtca
    15961 atgtagaagt tattccagga tttgataaag accctgtgtt tatcgagacg atgcttcgtt
    16021 tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct tgtggtgatg
    16081 gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt atctgtccat
    16141 atattgatta ttctttattt gaaagactga tcaataaagt aagcttttat gaagtttgtg
    16201 aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg ttagtaaatg
    16261 gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct gcaaacagtg
    16321 tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta gagactagag
    16381 aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt gaaatgattg
    16441 tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg tatgtagatg
    16501 aagatcatca agttcggatg atgtgtcttg gtcatccact gttagaagat cctacaccag
    16561 catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc tatcaaacca
    16621 ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat atgaagtatg
    16681 atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt cgtagcagtt
    16741 tctttgtgac attgaacggc ttgaatctgg ctcgtttcgt cacagaagat cgagtgttta
    16801 acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca agattatgga
    16861 tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac aaaaaactag
    16921 ctgagcaaat gatcaaagaa aatcgttacg gtactactgt gttttatgaa aaagaccggt
    16981 caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca agattcaaaa
    17041 agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt ttaggcggaa
    17101 tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg aaagctacaa
    17161 aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct gatcgtacag
    17221 cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat gatattgaga
    17281 aacaaaattt attgcagccg aacttcattg tgttgacttg taacaccgca cactactttt
    17341 ttgaagaatt acaagcggca acggatattc ctatccttca tatgccaaga gaagcagcaa
    17401 atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca gaaggaagta
    17461 tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg attcgaaaca gtgattcctg
    17521 atactgttct tcaagaaaaa atcaattatc tgatctatca tgagatcaag gaatcggatt
    17581 atttgaatca agagctatat tacgaaatct tagaagaagc ggtagagcgc ctaaactgtg
    17641 aaaaagttat cttaggttgc acagaattgt cattgatgca tgaatttgct gaagataacc
    17701 attatccagt gatcgatgcc caatctattt tagcagatcg gacgattgaa cgagcgctag
    17761 ctgaaagaag caaggcattg gatacagcat cggaaaagta aaaaaattgg atttggggca
    17821 aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa actggtaatg
    17881 atacgcccgg aatgatataa ttttactatg cacaatgtga gaggagttcg taattatgac
    17941 agataatttg caattagtca tcataacagg tatgagcggt gcagggaaaa cagtagctat
    18001 ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc gattgattcc
    18061 aaaattctgg gaattaatca aagaatcagg caaagtcaca aaaatggctt taggagtgga
    18121 tttacgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa tcgaaaatac
    18181 gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac tagtggctcg
    18241 ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca ctgaagggat
    18301 acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga tcattgatac
    18361 gacgacattg acaccacgac aattacgtga aaaaatcaat gagtcattca aatcaaaggg
    18421 agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt tgccgatcga
    18481 ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc cggaactaag
    18541 accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg atgaaacgca
    18601 atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat acattaaaga
    18661 aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc gttctgtggc
    18721 attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg tgacacatcg
    18781 ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac ctaccggata
    18841 agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat cctgaaaagt
    18901 ttgcgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga tgggggaagc
    18961 agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag aaatgtctta
    19021 gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg tttcaaaaag
    19081 gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc agtttccgag
    19141 atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca tgtagatgga
    19201 catgtttatc cttcttcaga aactcctttg accttgcatg ctgtctttaa agacggaacg
    19261 actgcagtag gagaatctaa aatcgcgatc gaccgtaaaa ccattgaccg tgtgtatgtc
    19321 tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc agcaattatg
    19381 gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct tccaaattta
    19441 gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt ctatatctgt
    19501 aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca tatacgtgtt
    19561 ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac ggaaaaagtg
    19621 cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt cgcacacgac
    19681 ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt ggaattgaaa
    19741 gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg tttagttttc
    19801 ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat gtcatttgct
    19861 gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc gaaagcggaa
    19921 ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca gtttattttg
    19981 aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt gaaagatcat
    20041 tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa gaacaatgtc
    20101 tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga tatcatggat
    20161 ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca aaaaatgcgt
    20221 tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga aacgagccgt
    20281 tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg cgatatgtta
    20341 aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat ttcttattta
    20401 aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa ctcaatgctc
    20461 cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg tctagtcaat
    20521 tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat cgaaaacatc
    20581 gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca ggaaatcgca
    20641 gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat gattccctca
    20701 ggtgcgatat caaaatcggg aattaatcac cgaattcgca aaataaatga atttgcggaa
    20761 aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag caggagatgc
    20821 gaatgtatca gtttttttta aaagttatac ttgcttttct tccgtcaaac tataattcca
    20881 tctttactgg agtaacataa tttggaaagg cttctattgt ttttaataca atcgtataaa
    20941 aagcggggag acaactgaat gaagatttta gttttgtaca taaatttttg cttaatatag
    21001 aagtcctgat atagataaga tcaagtattt atctataact ggcctgatta attaaaaaac
    21061 tggaaggaac tgctcgatca tccttgagaa ctgctcttct tacttctggt caagattctc
    21121 gtggttggtg gataaggatg gttgataagt tcattggatt agctagtcga aaacaaccag
    21181 gtgtaattaa tgtagaagtt ttaacaaggg gatggagtga tttattcagt gataatcata
    21241 agttcataaa tagtaattga gatgtatggt tggaatacgt tatacctgaa cttgaactac
    21301 caagatttgg tttagaattt gagaatcgat gtttataaag ggagggcaaa aatgcgtttt
    21361 tttacaactt ttactatcat tatgattgca gtacttttta tttttctaga tatagcaaag
    21421 cgaaatacag catttttatt atatcgggtt ttacttcgag caggtctcat aacatttata
    21481 tcgatcgttg gattttttct attcacagtt attgtattta tttggagaac tccagcacca
    21541 ccattgccag agattacata tggagaattt ccttttagac ttgagtatga attgaatgaa
    21601 gaattacatg tgatagaaga cacgttaata gttgaatttg atggatttgg aatgaacgaa
    21661 ggcataggga gatatcgtag atggacaagt cgtttagcca gcggagaaga tttagtgttg
    21721 ctgttagaag ttagtgataa taagcaaata ttttattttc caggacctgc taattattac
    21781 atgggagata ggttgaatgg ttataatcat acttttccta gtgcttcttt cattgaaagg
    21841 gaaagaggca ttactagaag ggatatttta catgacaaag aattattaga acaatttggt
    21901 ccgcttgatc aaaatacaat aaatgaagaa gaattgttaa atcaatataa tataaggcta
    21961 gtgaattggg aaatatctga accgattgtt aacaattttg gtgattagaa aacaattacg
    22021 ataaatagaa acaagccaaa tcatcagcaa gtaaaagctg atgatttggc ctttcttata
    22081 cgagtagtag gaattcatag cgaatattat tacgatcagt agatattttg atattaaagg
    22141 agtattacga actagtatgt gagaatgtag aggtgatttg cgaataatga gaaatttatc
    22201 aaatagaaac aaaatattaa tcattattgt tgttatagct gtgtttcatc tgggaacgaa
    22261 tgcggtttta agtagaataa tattgggtcc taagccacca agacctgcga ttacccgagg
    22321 ggagtttgat tttagattag aatatgaagt agatggggaa aggattgtga tagaagatac
    22381 aattgttgca ttatttgatg gattttctgc agatgcaggt agcatggcat ggtatcgcac
    22441 gtggcgtttg catttggcga gtgacaagag ggcacaacaa ttattgttac atgagttaga
    22501 tgatggtcga aaaatatatt atcattttgg aggagctgga ttttatatgg ggaagttagt
    22561 agcagtgtaa ctgtaacaag gaaagatttg ccaggtcttg gcattcgaaa ctccatattt
    22621 tatatcgagc gtgggagaga tacatctatt gggaacagga aagggttaac tcttgaagag
    22681 ttatataatg actttggaat ccgtcttgtt agttgggaac acaatccacc aatagaaaat
    22741 cgctttgaat aatcgaaatc tattaatcca tttcatcagc cagtgaatgt tgtaatggaa
    22801 caatttggtt tagtgttcaa aaccattgaa atactaggct gtgatcagca aaaatgtatc
    22861 tgaataaaaa agagagcaac tattaaatga gaggagttag gagttgttag ctttatacga
    22921 atcaaagtgg caaatacaga tgcgtatgca tttgtgtttg ccatcttgcg tttggtagac
    22981 gagataggta taagctaaag aaacaacttg tttaaaataa acgacctagc tcaacaaacg
    23041 accatctaat caactaacag catacataaa agcaactatt tttgaagtag aattgaaatc
    23101 gggataactt aaaaagtgat tgggatacta gtgtgaatca ccgtacataa atattactcg
    23161 ttaagggaat ttgagaaatt ttgtatgaaa atttaagaat tagttagtat gcgcaagtca
    23221 aaaatatgaa taaattattt ggtggagata atttgctcag cttgtcataa gaaatgagat
    23281 aatctggcag atgcaagctt ttggacacca tttattcgga ataaggaggc tgggacagaa
    23341 gcgtttaact ccaagaaata agaaggagtt gcttctgctt ccgccgttta taggtgttta
    23401 gagcgtgaga caaaagtgtt ctttactttt gtcctacgct ccttattttt ttattttgtt
    23461 tggatcaatt cttttaattc ctctattgaa atagattcat caaagatttc tttacccaag
    23521 atgacagtcg gaacaaatcg aatatgtgct tgctcagctt cgttgatgac agcttggctc
    23581 agctgttcgt ctttttgttc tgtcagcttt agtttatcta ctgcaaaatc cgctacttct
    23641 tggagagaaa gatgtttcca ctcgtcttga gtatcgaata tttgtttgat ctctttgatc
    23701 gctttttgac catcgctgat tgtgagatac cggtgcatca cgtttcctcg ctgcaagctt
    23761 tctttttctt tatcgaacaa tttgatcact cgctgcaatt gcccgctttg gactgcttct
    23821 tctaatagct cataagattc ttcaaaccat tgtcggcaat acggacaaga taggttgatg
    23881 aattcgacca tttttttagg agcattcgat gaaccataaa ggattccttt ttgtgtattt
    23941 gttttcgttg catcaatcac tgatatatcc atgttaaaac acctctttcg cttatagttt
    24001 atacatgtct gacaaaaaaa ggcaacgatt tagactgttt gtctaaaaat tagatgacca
    24061 attcaaatag acatatgtga aatattttta taaataaaag aaaaaatgga atgttttacg
    24121 ttataatgga acagataata agagagaata tgaacaagga tgatgaatca tgcgaaaaat
    24181 gaaaacaatg gatggcaata cggcagccgc atatatttct tatgcattta ccgagattgc
    24241 tgcaatatat ccaataacac ccagttcgac catggctgaa ttagtagatc aatggtcagc
    24301 agcaggaaaa gaaaatgtat tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc
    24361 aggagcagct ggtgtggtcc atggatcatt gaaaactggt gcactgacaa ctacatatac
    24421 cgcttctcaa gggctgcttt tgatgatacc gaatatgtac aagattgctg gggaactact
    24481 gccaagtgtt ttccatgttg ctagtcgggc agtgacgaca aatgcgttga atattttcgg
    24541 agatcacgga gacgtgatgg ctgcaagaca aacaggattt gctatgttag ctgaaagcag
    24601 tgtacaagaa gtgatggatt tagcacctgt tgcccatctt gcttctattg aaggagaagt
    24661 accttttttg aatttctttg atggattccg tacgagtcac gagattcaaa aaatcgaagt
    24721 tttggaatat gaagaattag ctcctctgat caatcaggag aaattagctt catttcgtaa
    24781 acgagggatg aatcccaatc atccgactgt tagtggtacg aaccagaatc cagacatcca
    24841 ttttcaacaa cgggaaacga tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa
    24901 atatatgaaa gagatcaacc gattacgagg aacttcttat gacttggtga cttattacgg
    24961 agcagacgat gcggaagaag tcattgtttc tatgggatca gttgcacaga cgattgaaca
    25021 aacgattgat tacttgaatg caaacggacg gaaaacaggt tttttgaata tccatcttta
    25081 tcgtcctttt ccagtggaaa cattcgtgca aaagttgcct catacagtaa aaacaattgc
    25141 tgttcttgat cgaagtaaag aaccaggggc aggtggagaa cctctcttat tagacgttca
    25201 aagtgcgtta tatgattctg aacttcgccc ggctgttatt ggtggtcgct acggattggg
    25261 gtctaaagat gtgactcccg accaaatctc agcagttttt gatgaattga aaaaagaccc
    25321 aagcatacgc aaaaaacggt ttaccatagg gatcgtggat gatgtgactt atcaatcttt
    25381 ggaaccaaag gaaagtctgg atttgacaga accgcaaaca ttccaggcca agttttgggg
    25441 atttggatct gacggaacag ttggagcaaa taaatcagct atcaagatta ttggagatca
    25501 caccgataaa tatgcgcaag gctattttta ttatgattcg aaaaaatctg gcggtctgac
    25561 tgtctctcat cttcgttttg gagatacgcc gatacgttca gcgtatcttg tcgagcatgc
    25621 ggaccttgtt gcctgtcaca caccagctta cctgcatagc tatgacttgg tcaaaggatt
    25681 gaagccaggt ggcatttttt tattgaatac tctttggagc gacgaacaat tagaaacgca
    25741 tctgccctta aaattaaaac gttacttggc tgaaaataac attagattct atacgatcaa
    25801 tgccatgaga ttggcccaag aagtcggttt gggacgccgg atcaatacgg caatggaaac
    25861 ggcttttttt aaattagctg acatcattcc ttttgatgag gtgcttccgc tgctaaaaga
    25921 agaagccctg aaaagctatg gacataaatc aatggctatc gtagaaaaaa acatacaagc
    25981 aatcgatcgg acgaccgaac tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt
    26041 ggagatgcct gaaaaaatca ataaaaggaa cactggttat atacaagaaa tacttgaacc
    26101 agtgaatgca caggaaggaa atcaactctc tgttggtaca cttgtagcaa atggaatgac
    26161 aactggcgaa atgcctttag gaacggcttc tatggaaaag cggggcgtcg ctttagaagt
    26221 acctgagtgg attagtgatc gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca
    26281 tgctgccatc cgtccatttt tagcagatga agaggaaatg gaagaagcgc cagaaggatt
    26341 tatcgtgaga gaaatgcgag gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga
    26401 agactgtaca ggttgtggtc tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt
    26461 gatgaagcct tatgaagaac agaaagaaca agctgtgaat tgggcttttg caatgacatt
    26521 aagacaaaaa gcaaatccag tcaaaggaaa gaatactgtc ttgtctacac aattcaatca
    26581 accgcttctt gaattctcgg gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt
    26641 attgacacaa atgttcggtg atcgaatgat gatcgcgaat gcgacaggct gttcttctat
    26701 ttggggcggt gcttctcctg cttctcctta tacgaccaat gaatgtggtc aagggcctgc
    26761 atggtcaaat tctttattgg aagataatgc tgaatttggt tatggtatgc ttctagcggc
    26821 acagactcgt cgcgaggctt tggcaagttc aatcaaagaa gtattgccaa aagtatcaga
    26881 agaactacga gaattgatgg aagactggtt ggtacacatg tttgagagcg aaggaacgca
    26941 gcaaagagca gcaaaactgc aagctgcgat cgaaagcgaa cgatcaattg tccctgaact
    27001 ggaggatata tacaagagaa aggatctatt cgtaaaaaac agccaatgga tgattggtgg
    27061 agacggatgg gcttacgaca ttggttttgg cgggatcgat catgtacttg ccagcggagc
    27121 agacgtgaat attcttgttt tggataacga agtctattcc aatactggcg gtcaaacctc
    27181 aaaagcaaca ccagcttcgg caatcgctaa attttcggct agtggaaagt atgcttcaaa
    27241 gaaagatcta ggaatgatgg cgatgacata tggaaatgtt tatgtggcgc aaattgcttc
    27301 aggtgccaat caaatgcaga cgataaaggc ttttgaagaa gcagaaaagt atcctggacc
    27361 ttctttaatc attgcctata cgccttgtat cacacatggt ttgcttggtg gaatgaaaga
    27421 atcgcttaaa gaagcaaaag aagctgtttc ttctggctat tggtcattat atcgatataa
    27481 ccctgcaatg gtcgaaaaaa acaagaaccc aatgactttg gactacaaaa aacctgattt
    27541 ctctgctatg cctgatttca tgaggaaaca agttcgcttt tcctcgctag agaacgcaaa
    27601 tccaaatctt gcaaaagctt tatttgaaaa gactatccaa gacgctaaaa cacgttttta
    27661 caactatgct gcactgacgg gagaattaga aaaaatcaag aaacgacttg aaccagagga
    27721 atctatcgat gtacccgtta agactagaaa agaaaaaacg acggaccttg aagcagaaga
    27781 aagaagagct gccagagcag aacgacgtaa aaaaatggaa caagctacaa aagaaaaaaa
    27841 ggaaaaataa cagaaaaaat ataatgaata attctctgaa gtttaaaaat atttattgca
    27901 gagaaaaata aaacgcatac ttgcaatttt cctgaaaatt atgcgttttt ctcaagaaaa
    27961 atacgagcag atcagcctct aaaattaaaa catatgatat actagagagg aaatctcaaa
    28021 agagaagaga ggctttttat gtcatttcaa atcggggaac tttttaattt ggactattgg
    28081 cagcaaatgt tttccggtga cttgttctct ctgaattttt tggtgaacat acttgatatt
    28141 ctggttgtct ggtatcttgt atacaaactg atccaactgc tgcgaggcac aaaagcgatt
    28201 cagcttctga aaggtgtagc tatttttatt gttatccgaa tcgtagcgga aatcattggt
    28261 ctgcacacct tgtcttggct gatgaaccaa gtcattacgt acggggtgat agctgctatc
    28321 gtgatttttc agccggaaat ccggagagga ctggaacatc ttggacgcag ttcgcttttc
    28381 aaacaatcaa aaagcgaaca gcaggaagat gagaagatga tcttgtcttt tgacaaagcc
    28441 atccaatata tgtccaagcg aaaaatcggt gcgctaatta ctatcgaacg gcatactgga
    28501 ttagatgagt atattgagac aggaattgca ttagatgcag atatcactgg agagctgctg
    28561 atcaatatct ttattcctaa tacaccgctg catgacggtg cagttattgt aaaagaagga
    28621 aagatcgcag tagcaagcgc ctatcttcct ctatcagaaa gtatgttgat tccaaaagaa
    28681 ttcgggactc gacaccgggc agctgtcggt ataagcgaag tcagcgacgc gattaccatc
    28741 gttgtctctg aagaaactgg ggatgtcagc attacattag ataatgaact gatggctggc
    28801 ttatcccagc aagaatattt ggcaattttg cgtagagaac tgattgctga agagaaaaca
    28861 aaaaacaaga aaaacgtgat tcaatctttc ctagacggta tgactaaggg aggggatcgc
    28921 aaatgatctc aaaagagaaa cggacaagca ttctttacag ttttattgca ttgctgttca
    28981 gtattgcgct gtacttcaat gcaaatggcc agtccgttcc aaatacgctt tctggaaatg
    29041 aagcgtataa tcaaacagtt actggggtgc caatcaagat ttcttatgat tcaaagcgct
    29101 actatatcca tggatatgaa aatgcagtga ctgtcaaact ttccagtgca aaccgagttc
    29161 agttgaatgc agaggcaaac gaagatacac ggatgttccg cgtcacagca gatatcacta
    29221 agctgggcga aggaacacat gaggttccgc taaaaattca aaatttgagc agtgctgtca
    29281 cagcaaagat cgaaccatcg actattacag taacggttga gaaaaaggta ataaaagatt
    29341 tcgatgtgga aacgttgatt ccttcaacgt tcacaccatc tggatatgaa cttgatgata
    29401 cttccgttag tcctaaaaaa gtctctatca ctacaggtga caaaacattg gcagaaatca
    29461 aacgagtagt agctgatgtg gacgcttcga tggtcacaga tgatgggatt aatagtgagg
    29521 tcccaataca ggcgctgaat gcagcagggg aagtattaag tatcgtctct gatccagtgc
    29581 aagtaacagt gagagctgat tcgatcaagc caagcaagtc ggtacgactt tatgggatac
    29641 agcaaggaac accagctgcc ggggtagaaa gctatgattt cagtttttca aaactggaag
    29701 cagaagtaag cggctctagc gatttgcttg catcgatcgg tgacagtatt gctgtaccga
    29761 tcaatgtctc aggtatcaat catcgaacaa ctcgtaaaat tgaaatacca gtagaagagg
    29821 ggttaagtat caagcccgaa tcagttagtg tggagattac acccgtgttg caaacgtcaa
    29881 gcagtagctc tgcttcgacg agcagtcgga cacaagaaaa tacaagtacg agtagaagta
    29941 tttcatctgc tgattcagga acggaaccat cgacaacaac catttcttcc agttcggaat
    30001 ctgggacaag cagctcgacc acaagtacag aagaaacatc agaaacaagc gaatcaacac
    30061 aaatgagcag aacaaattcc tcagaaaatt aagttaaagg agtaatttta caactatggg
    30121 taaatatttt ggaacagacg gcgtacgcgg tgaagccaac aaggaactta cgccagaact
    30181 tgcatttaag ctgggacggt gcggaggtta tgtcctaagc cagcatgaag aagaaggaaa
    30241 acgcccacgc gtgttagtag ggcgggatac aagaatctca ggtcaaatgt tagaagaagc
    30301 gctgatttca ggacttttat cggtgggtat cgaagtgttc cagctaggtg tgatctctac
    30361 accaggtgtt gcttatctga cacgtttgca aaaagcaagc gcaggggtca tgatttctgc
    30421 ttctcataat ccagcacaag ataatggaat caaatttttc ggtgcagatg gttttaaact
    30481 agtagatgac caagagctag aaattgaagc attattagat gctgaagtgg atgaattgcc
    30541 tcgtccatca gctgaaggat tgggaactgt agaagaattt ccagaaggct tattgaaata
    30601 ttctcaattc cttgtacaaa cgatcaacgg tgacctttcc ggtttgacca tttgtgtcga
    30661 tgcagcaaac ggagcaactt caacaagtgt taatcgtttg tttgccgatc ttgaaacaga
    30721 tttttatacc atgggcacaa atccagacgg gttaaatatc aatgatggcg taggctctac
    30781 ccatccagaa cgtttagctg agatggtcgt tgaaaaagga gcagatgctg gactagcttt
    30841 cgatggcgat ggcgatcgta ttattgcggt ggatgaactt ggaaacattg tcgacggtga
    30901 taaaatcatg tttatctgtg ctaaatactt ggcagaaaaa aatcgattga aaaaagatac
    30961 gatcgttacg actgtcatga gtaacttagg attccacaaa gcggtggaag gaatcggttt
    31021 gaaagatgtc gttacacaag ttggtgatcg ttacgttgta gaagaaatgc gcaaaaacga
    31081 ttataacttt ggcggcgagc agtctgggca catgattttc ttagatttta acacaactgg
    31141 agatgggatg ctttcaggaa ttcagttact gaatatcatg aaacagacag gtaagaaatt
    31201 atcagagctt gcagcagagg taacaatcta tccgcaaaaa ttagtgaata tccgagtaag
    31261 tgataaaaac ggtgcgatgg atgttccggc aatcaaaaaa gtgattgaag aggcagaagc
    31321 tgaaatggga tcagaaggaa gaatcctagt tcgtccttcc ggtacagaac cattacttcg
    31381 agtgatggca gaagcgccta ctgatgagaa agttaattac tatgtcgata aaatcgcggc
    31441 agtagtcaaa gaagaaatcg gactggatta agagatattt tcataaaaaa taaaagaaca
    31501 aggaccagtg tcagatgtct gacactggtc cttgttcttt tattattcta ctgtcactga
    31561 tttcgctaaa ttacgtggtt tgtcaacgtc gaatccccgt aatagcgttg cgtaatatgc
    31621 aagtaattga gtcggtacga ctgtggttag agtagttaga taagggtgga cagttggtag
    31681 caccagctgg tcactttcct tttctaatcc ctctgagaca ataactagtg ttttagcgcc
    31741 acggctttcg acttctttca ggtttcctcg agtatgcgca ccagtcactt cttcactgat
    31801 gattccaatc actggtgttc cttcttcgat caaagcaatc gttccatgtt tcaattcgcc
    31861 agcggcaaaa ccttctgcct ggatataact gatttctttc aatttcaaag cagcttcaag
    31921 agaaacgttg tagtcagcgc cacgtccaat gtaaaaggca tttcttgtat cgctgagata
    31981 tacttctgcc aactcttcta aataatcttt ttgggcgatg accgattcca tcgcattagc
    32041 aactaaactc aattcatgag tgatatcgaa tacaagagaa gtttcaaatt cttttttatc
    32101 accgatagct ttggctaata aagttagtac agctatttgt gcagtataag ctttcgttga
    32161 tgctactgcg atttcgggtc cagcatgcaa tagcagtgta aaggatgctt cccgcgaaag
    32221 agtagaacca gcaacatttg taatcgtaag tgaagggtag ccgagacggt tgatttttac
    32281 cagaacttgg cggctatctg ctgtttcgcc actttgactc aagaaaataa agaatggttt
    32341 ttttgaaaga agaggtgtgt tatagccaaa ttcactggct aaatgaacct caacagggat
    32401 ctgtgtcaat ttttccagag tctgtttacc tgctaacccc gcatggtaac tagttccgca
    32461 agcaacgata tagattcgat cgctatcaga caatgtatcg accagctttt gatcaagaga
    32521 gacacgattg tcttctgtta cgtatttttg agcgatacga cgcatgataa taggctgttc
    32581 gtcgatttct ttcagcatat aaaaaggata ggtacctttc tcgatatctg aagaatctac
    32641 ttgtgcttcg aatgaagaac gttctacgat gtcacctgca aaattttgaa taataacttt
    32701 ttctgatgtg acggtaacca tctcttcatc ttcaatctca acgaattcct tcgtatgagc
    32761 aagcatagcc attgcatcgc tggcaatcac attgaatcca tcgccgagtc cgattaatag
    32821 tggactttta tttttagcga cgtaaataac atcaggtgct gtccgatcaa tcaaagcgaa
    32881 agcgtaagaa cctttgatga cccgcaaagc ttttaaaaac gcctcttgcg ttgtagtatc
    32941 cttcgcgaaa gtttcaatta gatgagcgat aatctcagta tcggtctcac caataaagtg
    33001 gtcatttgct aaataagcaa ttttaagttc ttcaaagttt tcgattacgc cattgtgaac
    33061 gagcacgaag cgtccacttt gggaagtatg tggatgggcg ttttcttcag aaggttgccc
    33121 gtgagttgcc cagcgtgtat gaccaatacc tatatttcct gtagtatcaa tatttatttt
    33181 gtttttcaaa ttttgaattc tgccttgtgc tttgaccaga taatcagttg tgccatctga
    33241 gacaaagata ccagctgaat cataaccacg gtattctaat ttttctagac cgtcaatcaa
    33301 tcctggtgtt acatttttta atccaatcat tccaacaatt ccgcacataa aaaacatcct
    33361 actttctata atgaattcgt atgaatgctt ccagctgcag gaaaatcctt ttgttttatc
    33421 tttgcagata ttttttgtgg attttctgta gagtgcagtc cctagggcca tccgccgaat
    33481 ctttcgataa accctctcct cgtcatctgt ttacacagac ctggcgctat cctaaaaggt
    33541 ataactatcc tcctcttttg ttttttatct ggaaacattt aaaatcataa ccttttgcga
    33601 atgttcttgt caacaaaaaa agaataaaaa ataaattggt atatatcaaa tgctttgtca
    33661 aatgctcttt ttatgtcaaa aaataatggg tttttatcta tcatcttttt caggagttcg
    33721 gtacaataga aagtaatcgg atagaggagg agtaaaaatg gattcaaaaa ttgagatcat
    33781 gacactggga atgttaaaaa aacaattgtc caaattcgaa gcatcagctg gtgtatcaga
    33841 tgatacgaaa atatttttag atactggctg ggatagcatc caagaaatcg cacctgatgc
    33901 cttggaagtt gctgaagctc gtgagtttac ggtagaagat gaattgacaa aagagtcgtt
    33961 ttccgggtat gcgcgggaag aaaaagcaga gcggttcgac acttctgagc agtcagaaac
    34021 tgtgatcgtc ataaaaaact tgtattagaa aggagaaaga atgaataaga gacgttgggc
    34081 agcagttctg attgctgcag gattatttgt ggtttcgtta gtttcagcta gtttgactaa
    34141 gcctcaggaa aacgatcaaa ttgagggatt gaacagttgg ttatatgggg atgaagaact
    34201 agcttcaaat gtgttggaag agggagacag tacgcaaaaa atcgtaaaat tatcagtaga
    34261 tggaacgatt gctgcgggag ggtcttcagg tttatttgct tctgaaggtt acaatcataa
    34321 aaattttatg gaacaactga aggcgattga agaagatcca acagtcaaag gtatcttttt
    34381 agaagtaaat tctcctggtg gcggtgtcta cgaaagtgcg gaaatcgcta agaaattgga
    34441 tacgattcgt aaagaacatg atatcccaat gtatgtcagc atgaaaaaca tggcagctag
    34501 cggtggctac tatatttctg ctcaagcaga taaaatcttt gcaacagaag aaacagtgac
    34561 cggctcaatt ggtgtgatca tgtcaggatt gaattattca ggacttctag aaaaactggg
    34621 tgtggaagat acaacagtta aaagcggagc gttgaaagat atgggttcag gaacgcgacc
    34681 agagacaaaa gaagaagaag cggtgcttca agcatatatc gacagcgcat atagtcgttt
    34741 tgtagcaatc gtcagcgagg gacggaataa atctgaagaa gaagtcaaaa aaattgctga
    34801 tggaaggatt tatgatggtg tccaagcaaa agaagtaggt ttagtcgatg aattgggatt
    34861 tcctgaagat gctttaaaag ctatgcgaaa ggaacaacag cttgaagatg cggaactagt
    34921 agaatattct tccacgccat ctgggtttgc tagtacatgg ttaggaagta aactagcaga
    34981 attccaggga ttgaagcctt ctgagactag tcagatctta tccgttcttg agaatctagg
    35041 gacagcggaa tcaccaaaag caatgtatta ttacggaggt gagtaagcat gtcagagctt
    35101 tcaaatgaaa ataaggaaca agaaaaagat aagcaacagc agcctgctgt aggacctgac
    35161 tccaactttt cccaaaaggt actgagctct tttgaaagag agcctttgac gcccgaagaa
    35221 atcaaaaaga aacaacgtaa atggaaaaag tatacggaca caagaaatga accgccaaaa
    35281 gcaaatgatt ttcctaagta tttctatgca ggttttttta tccgaatgtt tgctttcttg
    35341 gtcgatcttt tgtgtatcgg agctattacg cgtttaacac ttggtattgc caaaaatcta
    35401 ggttggctta atttttcaga ttcttatctg agtatctacg gattcacagc cttacttatt
    35461 tatctagcgt attttatttt attgacgaaa ttcaatcatg gtcaaacgat tgggaaaatg
    35521 atctttggta ttcgagtcat cagctttgat gaagaagaac ttagctggtc aaccgtttta
    35581 gttagagaag gtgcttgccg gttcattcta aaatttcctt tgctgatgtt agggtatctt
    35641 ccagcaatct ttaatcagaa aaagcaacat ctaggcgatt ttttcactaa tacaagtgtt
    35701 gttacgatca atctgatcaa agcattcaac caggaagtca acagctgatg agcaataaaa
    35761 agcagtaaca gttgaaagca ccttctaaaa gtcagatcta aaatctagct tttagagggt
    35821 gcttcagtta tttttaattg tcatgctttt tttgttttgc tactattgaa catgagctga
    35881 acatgagcaa gaggaggaca ctgcgtgaag ccgattgatt ttccagaaaa atatgagaat
    35941 ttaatgcgag tagcacaaca agcactagca aaccagcaat atcagcaggc aaaagaatta
    36001 tttcaacgag cttacgagtt gaaagaaagc tttgaggcta atagcctgct tgttttttgt
    36061 ctttacgagt tggacgagaa aaaagaagca ttgaagcaag cactcttgca tgaaaaacag
    36121 tatcttcaaa atgaggagtt tgctgaattt tattttgact tgttaatcag tacacaagat
    36181 tttttatatg ctagaaaatt gatagcaagt acagattttt atgaagcttt cgagcagcgg
    36241 gtcattgaaa aaatccaatt tgcagaagaa ttatccggac aaatggagag gcagaagatc
    36301 aaggcactcc acaataaatc ggaggaactt ccttcgttgg aacctgcacg tcagcttgcg
    36361 tctattgatg agatcgaaca gcttccttat catgagttta tccaaacagc gagtaaactt
    36421 attgtatcgc cagaggtcca tattttagca agggcaaaat tactggagac acttaggcaa
    36481 ttgaacgaat gccatcctgt tttctatttg acgattgaag aaaagttagt caaagtcata
    36541 ccaaaagatc tgccaaagcc gcagcagcag gcaagttacc agcaattatg tgtattttca
    36601 gatcgttacg gtaatgaaga tgcgctgctt agttccgtgt taaatgaaga attcacgttg
    36661 caatctgcaa ttgtttatcc tgtctatgat acatacatcg aagacccgaa gcgctggttt
    36721 caactgactg tagaggcgtg tacaggaaaa acgatgtatg aaggtacaga agatgaaaaa
    36781 caagattttt
//
LOCUS       DAXWLG010000009        34295 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXWLG010000009 DAXWLG010000000
VERSION     DAXWLG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.009"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(201..1127)
                     /locus_tag="ACGE4T_000329"
     CDS             complement(201..1127)
                     /locus_tag="ACGE4T_000329"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFF8385645.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(1212..1973)
                     /gene="pflA"
                     /locus_tag="ACGE4T_000330"
     CDS             complement(1212..1973)
                     /gene="pflA"
                     /locus_tag="ACGE4T_000330"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFF8385646.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(2217..4448)
                     /gene="pflB"
                     /locus_tag="ACGE4T_000331"
     CDS             complement(2217..4448)
                     /gene="pflB"
                     /locus_tag="ACGE4T_000331"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFF8385647.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(4725..7175)
                     /gene="parC"
                     /locus_tag="ACGE4T_000332"
     CDS             complement(4725..7175)
                     /gene="parC"
                     /locus_tag="ACGE4T_000332"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFF8385648.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(7187..9232)
                     /gene="parE"
                     /locus_tag="ACGE4T_000333"
     CDS             complement(7187..9232)
                     /gene="parE"
                     /locus_tag="ACGE4T_000333"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFF8385649.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(9398..9832)
                     /locus_tag="ACGE4T_000334"
     CDS             complement(9398..9832)
                     /locus_tag="ACGE4T_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFF8385650.1"
                     /translation="MSVKNPTQETIFNYLEETKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            9959..10597
                     /gene="plsY"
                     /locus_tag="ACGE4T_000335"
     CDS             9959..10597
                     /gene="plsY"
                     /locus_tag="ACGE4T_000335"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFF8385651.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(10729..11604)
                     /locus_tag="ACGE4T_000336"
     CDS             complement(10729..11604)
                     /locus_tag="ACGE4T_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFF8385652.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(11686..12477)
                     /gene="codY"
                     /locus_tag="ACGE4T_000337"
     CDS             complement(11686..12477)
                     /gene="codY"
                     /locus_tag="ACGE4T_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFF8385653.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(12495..13895)
                     /gene="hslU"
                     /locus_tag="ACGE4T_000338"
     CDS             complement(12495..13895)
                     /gene="hslU"
                     /locus_tag="ACGE4T_000338"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFF8385654.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(13909..14457)
                     /gene="hslV"
                     /locus_tag="ACGE4T_000339"
     CDS             complement(13909..14457)
                     /gene="hslV"
                     /locus_tag="ACGE4T_000339"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFF8385655.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(14494..15399)
                     /gene="xerC"
                     /locus_tag="ACGE4T_000340"
     CDS             complement(14494..15399)
                     /gene="xerC"
                     /locus_tag="ACGE4T_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFF8385656.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVIYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(15597..16973)
                     /gene="trmFO"
                     /locus_tag="ACGE4T_000341"
     CDS             complement(15597..16973)
                     /gene="trmFO"
                     /locus_tag="ACGE4T_000341"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFF8385657.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(16942..19020)
                     /gene="topA"
                     /locus_tag="ACGE4T_000342"
     CDS             complement(16942..19020)
                     /gene="topA"
                     /locus_tag="ACGE4T_000342"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFF8385658.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(19142..19999)
                     /gene="dprA"
                     /locus_tag="ACGE4T_000343"
     CDS             complement(19142..19999)
                     /gene="dprA"
                     /locus_tag="ACGE4T_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFF8385659.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(20055..20822)
                     /locus_tag="ACGE4T_000344"
     CDS             complement(20055..20822)
                     /locus_tag="ACGE4T_000344"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFF8385660.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(20815..21675)
                     /gene="ylqF"
                     /locus_tag="ACGE4T_000345"
     CDS             complement(20815..21675)
                     /gene="ylqF"
                     /locus_tag="ACGE4T_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFF8385661.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            21935..22333
                     /locus_tag="ACGE4T_000346"
     CDS             21935..22333
                     /locus_tag="ACGE4T_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8385662.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            22570..23112
                     /locus_tag="ACGE4T_000347"
     CDS             22570..23112
                     /locus_tag="ACGE4T_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8385663.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(23195..23746)
                     /gene="lepB"
                     /locus_tag="ACGE4T_000348"
     CDS             complement(23195..23746)
                     /gene="lepB"
                     /locus_tag="ACGE4T_000348"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8385664.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(23898..24125)
                     /locus_tag="ACGE4T_000349"
     CDS             complement(23898..24125)
                     /locus_tag="ACGE4T_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFF8385665.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(24122..24640)
                     /gene="msrA"
                     /locus_tag="ACGE4T_000350"
     CDS             complement(24122..24640)
                     /gene="msrA"
                     /locus_tag="ACGE4T_000350"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8385666.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(24660..25373)
                     /locus_tag="ACGE4T_000351"
     CDS             complement(24660..25373)
                     /locus_tag="ACGE4T_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFF8385667.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(25376..26272)
                     /locus_tag="ACGE4T_000352"
     CDS             complement(25376..26272)
                     /locus_tag="ACGE4T_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8385668.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(26430..27272)
                     /locus_tag="ACGE4T_000353"
     CDS             complement(26430..27272)
                     /locus_tag="ACGE4T_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8385669.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            27404..28057
                     /locus_tag="ACGE4T_000354"
     CDS             27404..28057
                     /locus_tag="ACGE4T_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFF8385670.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(28120..28647)
                     /locus_tag="ACGE4T_000355"
     CDS             complement(28120..28647)
                     /locus_tag="ACGE4T_000355"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8385671.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(28667..29614)
                     /locus_tag="ACGE4T_000356"
     CDS             complement(28667..29614)
                     /locus_tag="ACGE4T_000356"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8385672.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(29626..31509)
                     /locus_tag="ACGE4T_000357"
     CDS             complement(29626..31509)
                     /locus_tag="ACGE4T_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8385673.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(31672..33750)
                     /locus_tag="ACGE4T_000358"
     CDS             complement(31672..33750)
                     /locus_tag="ACGE4T_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8385674.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(33763..34038)
                     /locus_tag="ACGE4T_000359"
     CDS             complement(33763..34038)
                     /locus_tag="ACGE4T_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385675.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
BASE COUNT         9154 a         7254 c         5915 g        11972 t
ORIGIN      
        1 tttttgaata atagttcata tatgttctct tttctatccg ttttatttta cttaaaaggc
       61 ttttacgtaa aaaggaggaa gaacaagagt cataattgac tgttattctc ctccatttct
      121 tcggccttta gtatgttcta tgtttctttt tatatcaatc gcttatccat tcattttgaa
      181 tgcttcatac taccatttga ttagctaaat acagccgtca attgaggtac gacttgtttt
      241 ttacgtgaaa cgacaccttt aagcaaagca cggttgttgg tcaaagttgt ttggaaagct
      301 tcttctactt tttcttttcc tttaccagca acaagtaatt ctgaatcact gtctaagatg
      361 tttgtgacaa ctaatacgaa cagatcgtag ccatttgctt tcatttcatc ttccattgct
      421 gcttctagct ctgcttgtcg atccatcact tcattaaggt ccactgtatt gacttgagcg
      481 atacgaacgt tggaactgcc cattgggaaa ctttttgcat ccaaatcaag aagaaccgca
      541 gctgatttat cacttagatt tgttcctgct ttcaacattt caagtccata agtgtttaga
      601 tcgacttcag cgattgctgc aagttctttt gcagctgctt catcttcagg cgtacatgtt
      661 ggtgatttga acaacaatgt atctgaaatg atagctgaga gcatgattcc tgcgatttct
      721 ttaggaattg ttacttggtt ttctttatat agttttaaaa taatcgtact tgtacatcca
      781 actggttctg cacgatagta caatggatta gctgtttcaa agtttgcaat acgatggtgg
      841 tctactacag ctaaaatatt tacttcgctg atatctgaaa tactttgttg gaattcatta
      901 tgatccacaa gcatgacatc tgcattttct gctttagctg actctactac tcgtggtgca
      961 gatagctgaa agtggtctaa tgcgtattgt gtttcttcat taggtgtacc tagagcaact
     1021 gcttctgttt ctttacctaa ttcattttgc aagtgtgaaa acgcaatggc tgccccgatt
     1081 gcatctgtat ctggattttg gtgaccaaaa actaaaattt ttgacatgtc attcgctcct
     1141 ttgtgatttc tcctatttaa taatagcaaa aaaacggaaa gaagcaatac ttatcttctt
     1201 tccgatgcga cctatcgcgt taaatatttt gtatactctt ctgtatgaag caatcgttta
     1261 gcattttcga cacgttcttt tgtaggtggt tcgatccctt ctaatggata tttcaaacca
     1321 agtgtttccc acttgtattt tcccatcgta tgataaggaa gaatctctac tctgtctaca
     1381 tttgacaagg tttgaataaa gttgtctagt cggatcaaat attcatcata atcacttcgc
     1441 tcaggaacta gtacatggcg aatccaaacc ggtttgttga tttttgacaa atattgagcc
     1501 atttctaaaa tattttcatt gtcccaatgc gttagttttt tatgctcttc attatcgatg
     1561 tgtttgatgt caaataacag taaatctgta tacttcatca attcttgaaa acgtgaaaag
     1621 aaaggttctt catacgtaaa aggttttccg catgtatcca atgtagtatg catcttccgc
     1681 tcttttgctt ttttaaatag ttcgatcaaa aaatcgatct gcaatagagg ttctccacca
     1741 ctaacagtaa taccaccttt atctccccag taaggtctga agcgttctgc tttatctaaa
     1801 agttcatctg ctgtgtattc tttccctccg ccaatattcc aagtatctgg attgtgacaa
     1861 aattggcaac gcatgcggca tccttgcata aacacgacaa agcgcagtcc aggtccgtct
     1921 actgaaccga aagtttcgat agaatgaaca tagccgattg ttttttcttc catttaagtt
     1981 cacatctttt ctttgatttc attttaagta caagttagct gtcgaagcga agctttctga
     2041 acaaaatttt tcgcaattgc cgcttcgtta tgtgtaaaat ttacagggac ggaacataca
     2101 agtcctgccc ctgtaaatcg tttctttcaa ataagcatac tgattattcg attttgatgc
     2161 gatggctatt caacgtcctt tcgacgctaa gtcgaaaaac ttttttcacg aactttttac
     2221 attctgtcgt gtgaagttct catcaagaca tctaattgtt gttctcttgt caagtcacgg
     2281 aatttaactg cataaccaga tacccggatc gtcaagttcg gatatttcat tggattttcc
     2341 attgcatcac gcaataaatc attactgaat acattgacat tcaaatgata agctcctttt
     2401 gagaagtatc catctaagac attacgcaag ttttcgattc ttgtatcttg ttcttttcca
     2461 agacctgaag gattgattgt ttgggtattt gaaattccat ccaatgcaca gccgtaattc
     2521 attttagctg ttgagttcag gctagctaat aacccacttt gttccgcatt gtaacttgga
     2581 tttgctcctg gcgcaagtgg ttttccagct tcacggccat caggagtatt tcctgtattt
     2641 ttaccgtaaa caacatttga agtaatcgtc aataatgaag ttgttggttt tgagttgcgg
     2701 taagttttat gacgtttgat ttgtcccatg aagtattcca agatccatga aactaattca
     2761 tctgcacggt catcatcatt tccgtattta gggaagtcgc catccacttc aaaatcaact
     2821 gctattcctt tttcattacg gattgctttt acttttgcat atttgatcgc tgacaagctg
     2881 tctgccgcat gagagatccc agcaatacct gtagcaaatg tgcgttgcaa gtcagaatcc
     2941 atcaaagcca gctgagctgc ttcataagaa tatttatcat gcatataatg aatgatgttc
     3001 aacgtattga catatagttc tgctaaccaa tccatcatat tcatgaagtt atgtttcact
     3061 tcttcaaagt ctagtatttc tgatgtgatt ggacgatatt ctggtccaac ttgggcgcca
     3121 gtcacttcat cgactccgcc attgatggca tataatagtg ttttagccaa gttagcacga
     3181 gcgccaaaga attgcatgtc ttttccgaca actgttcctg atacacagca agcaatcgca
     3241 caatcatcag aaccccattg ttcacgaagc tgattatcat tttcgaattg aatcgaagaa
     3301 ctttcgatag cgattctgct agcatatgtt ctgaatcctt ctggaaggtg ttcagaatac
     3361 aaaacagtca agtttggttc tggtgaaggt cccatatttg ttaatgtatg aagcagacgg
     3421 aaatcattct ttgtaactaa tgaacgacca tctaatccca tacctgcaat ggataaagta
     3481 gcccagatag ggaatcctga gaataattgg ttatattcag gtgtacgcgc gaatttgacc
     3541 atacgtaatt tcattactaa gtggtcgatc aattcttgtg cttctttttc tgtgattact
     3601 ccattatcca aatcacgttg gatatagata tcaaggaatg ctgagatacg tccgattgac
     3661 atcgcagcac cattttgtga tttgattgct gctaaatatc cgaagtatag ccattggaca
     3721 gcttcttttg catttgcagc tggtttagaa atatcatagc catatttcgc agccatttct
     3781 ttcatttgcg ctaatgcacg atattgttca gtgatttctt cacgtaaacg aatcacttct
     3841 tcactcattg tatgataacc agtcatatca tgatcttttt tcttttgttc catcaaatag
     3901 tcgattccat acaatgccac acgacgatag tcaccgatga tacgtccgcg accgtaagcg
     3961 tctggaagcc ctgtaatgat tttgttttta cgagcagcac gcatttcagc agtataagcg
     4021 tcaaatacac cttgattatg tgttttgcgc cagtcagtga aaatatgtgt caattcatcg
     4081 tttggtgtat agccattgct ttttagcgca ttgttagcca tattgattcc gccgaacggc
     4141 atgaatgctt gtttcaaagg tttgtctgtc tgtagaccga cgattttttc caaatcttta
     4201 tccaaatagc ctgcatcgtg tgaagtaata gttgatacga tttctgtgtc catatcatag
     4261 acaccgttac gctcgaattg gatatcattt aacttttgca attcatccca taatttttct
     4321 gttgcttctg ttgggccttc caaaaagctg tcatcaccgc gatattcagt gtagttattt
     4381 tgaataaagt ctctagtgtc tacgccttct ttccatttgg tacctttaaa tccattccat
     4441 tgctccattg tatggacctc ctaaatttct gttttatgta acagttttta cgtgattagt
     4501 ataacacagc accctatctt tgcaagggtt ttcttgtttt ttatttaaca atttttatgt
     4561 tttatttaca tatatttaaa tcaacgttga cataccaaca tttaagcatt gtgaacactt
     4621 tcatcttttt ttgcaaacgt ataaggattt attaaaaatg aatgaaaaag agacgccacc
     4681 tgtattaata cagatggcgt ctcttcatta aaatactaca acagttacga ctcgatgaaa
     4741 atctcaggag catcgataac ttcgatgact tctccacctt ttttctcatc catcgcaaat
     4801 gatccattcg atgttcgatc acctataggg aaatcagttg ggtcaatcgt taactgctgc
     4861 cctttttgat tcatcaaggt tatttctttt gatcttcctt ccgacatata gataatacgg
     4921 tgcggatttt ttttcagctc acgcatgacc gtcactcccc gcttcgcacg tgaagtttgg
     4981 ctgatttctt gagcaagcat gcgtttaaca gctcctcgct gagtaacgat tacgatcggc
     5041 gtatcacctt ctgctaacac aagcagcccg ttgataatcc tatctccatc tttcaaattc
     5101 atagacttca ctccagctgc ctttgctcca acaacgggaa catctgttaa aggatagcgc
     5161 aaacccattc cttgatgcgt tactaagaaa acatctaagc ctggctgatc ttctttcaga
     5221 tacacagcaa tcagttcatc ttcttccgcc tttagcttca tacagctgag cggacggctc
     5281 ttataggtgc gccatggttc gaactctgtc attcgtgttt gcttgatcaa tccattcttg
     5341 ctgatgaata caaatgtttt ctctgcttga attttttgat atgggaaaac agctaaaatc
     5401 gattcgtcta cagacagatt ggagattgtt tgagaaatgt gctcaccagc atctttccat
     5461 ttcaaatcgg gtagttcatg gacagggcgg tagatcacat ttgctttatt tgtgatcaga
     5521 agaacatggt ccaatgtact caattcacct gagtatagca agtagtcgcc ttctcgcatc
     5581 ccgatttctt ctggtttgga agcactataa gaacgaaggc ttgttcgctt cacatagcct
     5641 tctcgcgtca cactgacaat tacgtcttct tgtgccacta agacttgcgt atcaattttg
     5701 atttcttcga tttcgtcttc gattgtcgtt agacgagcgg aagaataatt ttttttcact
     5761 tctcgcaatt cttttttcat aacagagaaa agctctttgt catttgaaag gattttattc
     5821 aactctgtga tttgcgcaat caagctttcg gattcctttt gaagctctgt gatatccgtg
     5881 ttcgtcaaac ggtataattg caatgtgaca attgcttctg cttgttcttc tgtaaattga
     5941 aagacgtcga ctaaattctt tttggcgtct tttttgtctt tactttcacg aatcgttgcg
     6001 attacttcat ccagaatcga taaagctttc atcaatcctt ctacgatatg ctgtcttttt
     6061 tgagctttgg ctaaatcgaa ttgactacgt tttgttatga cttgctttcg atgctcgata
     6121 taacttctta aaatgtcttt cagtccaact tgatgcggag tcatatggtc gatagcgacc
     6181 atattgaaat tgtaattgat ctgtagctca gtgtttttga acaaataatt caaaattcct
     6241 tgagcattgg cgtctttttt caattcgacg acaatttgca gacctgttct atcactttca
     6301 tctcgtactt cagctattcc atcgattttc ttattcaaac ggatctcatc catttttttc
     6361 accaaggtcg ctttatttac ttcatatggg atttctgtga tcacgatttg ctgtttatta
     6421 cccttcattg cctctatctt tgttttcgaa cgaagaataa cttttcctcg tcctgtttca
     6481 taagcttttt tgatttcatc ttttccttgc aagatccctc ctgttggaaa gtcaggtcca
     6541 ggaatatatt ccatcaattt ttcaaggctc gcttgtggat gatcgatcat atagattgtg
     6601 ccatcgatca cttcagctaa gttatgagtc gggatttctg ttgcatatcc agcagatata
     6661 cctgtcgatc cattgacgag aaggtttggg tatcgagctg gaaggactgt tggttctttt
     6721 tctgtatcat caaaattcca cacgagatcg actgtttctt tctcgatatc tgctagcatc
     6781 tctccactta acttggataa tctggcttca gtataacgca tcgcagcagg tggatcaccg
     6841 tccatactcc cgttattccc gtgcatttcg atcagtactt cccgcaactt ccagtcttgg
     6901 ctcatgcgga ccatcgcttc ataaatactg atgtcaccat gaggatgata attccccatg
     6961 atatttccga cagattttgc tgatttacgg aatcctttgt catacgtatt gccatctttg
     7021 ttcatggcaa ataatatccg gcgttgcact ggcttcaatc catcacggat atcaggcagt
     7081 gcacgttctt ggataatata tttggaataa cgtccgaagc ggtcacccat cacttcttct
     7141 aaagttaatt cttgtacttc ttgtcgattt tccatcaggt gacctcctat tctacttcaa
     7201 ataactgatt gttttcattt ttatctgctc gttcttcatc taataaatca ttagaaactg
     7261 aaggagagat ttcagtatcc tcttttttat ctaagatact gccatcttct tcgagcgtaa
     7321 attgcacgtg attttcgatc cattttcttc gaggttctac tttgtcaccc atcagtgtgg
     7381 ttactcgacg ttcagcttgc gcagcatcat cgatacggac gcggatcaac gtacgggaag
     7441 tcggatccat cgttgtttcc cacaattgtt ccgcattcat ctctccaagt cctttatatc
     7501 gctgcagcat atatcctttg ccgacttttt taatcattgc tgccaattcg tcatctgtcc
     7561 acgcatattc aatgacttgc ttctttcctt gtcctttgga tactttatat aaaggcggta
     7621 acgcgatata tactttccca gcttcaatca gcggtttcat ataacgataa aagaaagtaa
     7681 gcagtaatac ttgaatatgg gcgccgtctg tatccgcatc ggtcatgatg attactttgt
     7741 cgtaattaca gtcttcgata gaaaactcag gaccgactcc tgcaccaatc gtatagatca
     7801 tcgtattgat ttcttcattt ttcaagatat cttgcatttt agctttttcg gtattgatga
     7861 cttttcctcg taaaggtaga attgcttgga actttctgtc tcttccttgt ttagcagatc
     7921 cgccagcaga atccccttcg actaaataca attcattttt cttagggttt cgggattgtg
     7981 cgggtgtcaa ttttcctgaa agaagagatt cgcctttttt acgcttcttc ccatttcggc
     8041 tttcttctct ggctttacgt gctgcttctc tcgcttctct ggctttgatt gctttacgga
     8101 ccaacatctg gctcatttcg ctattttcct gcaaataaaa gcccatttgt tcactgatga
     8161 cgttatctac tacggtacga gcaactggcg taccaagttt ttcttttgtt tgcccttcaa
     8221 attgcagaag attttcaggt acacggatag aaagtacagc agctaaccct tctctgaaat
     8281 cactaccttc cagattcttg tcccgttctt tcagcaaacc aacttttctc gcatattcat
     8341 tataagattt cgtcatcgcc gctttcatac cagcttcatg ggttccccca tcttttgtcc
     8401 gaacgttatt gacaaaagat aaaacatttt ctgaataacc gtcattgtat tgataagcga
     8461 cctctacttc gataccttct ttttctcctg aaaaataaac aactggtgtc aatgtatctt
     8521 tttcttcatt cagataatca acaaattctt tgattccttc ttcgtaatga aaaatttcct
     8581 tgactggttc ttctccacga agatcagaaa gagagatttt aacgcctttt agaagaaagg
     8641 cagattctct taaccgttcg gctaaaattt catatgaaaa tttcgttgta ctgaatattg
     8701 tatcatctgg taaaaaatgg acagtcgtcc cgtttgcttt ttttgttttt cctatctttt
     8761 tcaaagtacc gtcaggttta ccgccgtttt taaatttctg ctgatactct acgccatctc
     8821 taacgatcgt tacaattagc catttcgata aggcatttac cacactggct cctacaccgt
     8881 gcagtccacc agaagttttg taccctcctt gaccaaactt tccgccagca tgcagcacag
     8941 taaaaatcac ttcaactgtt ggtattccgg aagcatgcat tcctacaggc atcccacgtc
     9001 cgctatctgc aacagttata ctattgtctt catggatggt cacatcaatc tcactgccat
     9061 aacctgataa tgcttcgtca actgcattat cgacgatttc atagactaaa tgatgcaagc
     9121 ctcggctgtc agtggatcca atgtacatcc ctggtctttt tctaactgcc tctaatcctt
     9181 ccaatacttg gatggacgag tcattgtatt cgttgtttac tttttttgcc aaggcaaacg
     9241 ctccttcaat aatactttgc ggcaaaaccg caccacgatt atcatactag aaaaggtcgt
     9301 gaaaaaaaag agggtagacg agaaatcaat tttaattatc tcctaaccgc tttttcgaca
     9361 ttcccctcta tcgatcatct cttctttgtg gtcattttta tttttggttt tctttcaact
     9421 taccaagttc aatcttaata caacgatcca ttacgatatc tgtacgtcct gcttttctta
     9481 gcatctcagc tgcttctttg ttttcaatcc ctaattgtgc ccagaacact ttagcatcag
     9541 ttttcaaaaa atcacctgct atttcaggta aaaattcact ccgtctaaat acatctacga
     9601 tatcgattgg atcatcgatt tctgtcaaat tcgcatagac tttctcgcct agaatttctt
     9661 caccagccaa taatggattt actggtatga ttttatatcc attgtcttgc aatacctgtg
     9721 caaccatgta acttgttcgt tctggtttat tgctcaagcc tacgacggca atattttttg
     9781 tttcttccaa atagttgaaa atcgtttctt gtgttggatt ttttacagac atttctgcca
     9841 ccccttgctt ttctttttat tccatctatt atacttgtat ctttcatttt ttcaattgtt
     9901 cctgttacct attcatgcta aaataaagga tagttttcaa cttgaaagag ggtaaaaaat
     9961 gaaattgatc ttattactta ttttagcata tcttttaggt tctattcctt ccggtgtctg
    10021 gattggcaaa ttgtttttca aaaaagatat ccgacaacac gggagcggta atactggaac
    10081 gaccaataca tttcgtgtat taggcaaaac agccgggatt gttgtcctgc ttatggatat
    10141 tttaaaaggt acattggcta cttgtttgcc gatgatcttc cacctttctg ggattaaccc
    10201 tctttggttt ggggtatgcg ccattcttgg ccatacattt ccgatttttg ctgggttcaa
    10261 aggaggaaaa gctgttgcga caagtgcagg aatgctttta ggatttaatc ctgtattttt
    10321 tgtttattct tgtatcatct ttatcgttag tttattttgt acaagtatgg tgagtttgag
    10381 cagtatgatc agtgcagtat tgatcacttt atctacgatc gtccttcctt atgtagctcc
    10441 tgtgattttg gctaagccca attggttact gacgatcatt gcctttttag tttcttcctt
    10501 tatcttttat cggcataaag acaatatcaa gcgtatcaga aaccatactg aaagtcgaat
    10561 cccgtttggt ttgaatagca gcaagaaaaa atcctgatac tttttcattc caaagagata
    10621 aaacgggagt gtgacaaatc gatgtcacac tcccttatta cttacaggat agctttttta
    10681 cacaccttgg taaacggtgt tccctgctaa cttctcggtc acaacctctt acttcactgt
    10741 gatggaatat tttgtcttga agatttcatg ggcacctagt tgattgatcc ctactttttc
    10801 catcaagttt ccactactgt tcaccgtgtc agcgattccg caccacggtt cgatacaaac
    10861 gaaaggcgct tcttttggat aaggagacca gatccctaca taaggcatat ctttatagct
    10921 taaagttacg ctatgggaag atttttcact tcgaatggcg tacgcattta gaccccttgt
    10981 ttcaaaaacc aaggcatctt gctcaaaaag ttcacgattc aaagccagat tcgtatttgt
    11041 ctgacctaac gtcttttgat ccatatcgat gaatgggcct ttcaatggta ggcgtaatct
    11101 tgatttcaat ggagaaaatg ccagatagaa atcttcaaat gacaatcctt cttcaagagg
    11161 tacattgaaa gcaggatgtc caccgataga aaagaacatt tctttttcac cggtatttgc
    11221 cacttgataa tgaaccgtga tgccgtctcc tcctaattca tagctgatca ctaattcaaa
    11281 atcaaatgga tacttttcta atgtctccgg cgtactcctt aagatgaaac tagccatttc
    11341 ttgtccatgt tcaatgactt caaaatccat atcccgagca aatccgtgtt gccccatttg
    11401 atatgttttc ccttgataaa catactgatc atcttttaaa cgtccaacaa aagggaataa
    11461 aaccggtgca tgtctgcccc aatacgctgg atcaccttgc cagatatact ctaaaccatg
    11521 ttcttttgat ttcagactga tcaattccgc accgtgttca gcgatttcgg cgatcagctg
    11581 gtcattttga ataataacag tcattgccat tcctccaact tcgacttact ttttaaaatg
    11641 ggaaggctgg aacaaacatt tctatctgcc ccagtcccct tacttctaat aactgtgttt
    11701 tgctaactcg tctttaaaac gggaattcaa tacttttaaa taagttccct tcatgcctaa
    11761 cgaacgagat tcaatgattc cagctgattc caattttcgt aaagcattga cgatcacaga
    11821 acgagtgatg ccgatactat cggcaatatt agaagcagtc agacgacctt catctccatc
    11881 caatgcttca aagatagctt gtacagcctt caattcgcta taagataatg tgttgattgc
    11941 catttggaca gccgttgcac tccgtacatc tgcttcgatg cttcgagact tctgataaag
    12001 gatctgcata ccgacaaccg tagctccata ttctgctaag actagatcgt catctgcaaa
    12061 cgattgttcc atacgagata agataatcgt acccaaacgt tcgccagctc caaagatcgg
    12121 aacgacagtc gtcagaccaa atggatattt ctctcttgac tctaccggaa atgcagtaat
    12181 atcactagta attgggatat tagcttcagt tttcatcaac tgtgccactg tatcagtata
    12241 agattcagga aattgtttct caacaaacat tgttttgatt cgttcattgt tcacatcgtg
    12301 acgttcatta taacctagta atactccgtc acttcctatg atataggtgt tgctgtcaag
    12361 aataccacct agtgtgacag ccattttatc gtaaggcaaa tctgcttgca tgtcaaaagt
    12421 attcttttgc tgcaaaagtg tgtttatctt gcgggttttc gttagcaagt ctgtcattgt
    12481 cgtcctccga ttttttataa aatatagcga cttaaatctt catttgccgc aattgagcca
    12541 agtttgtcat ccacatatgc ttctgtgatc gttatttctc ccatttgcat atctggtgct
    12601 tcaaataata gatcttctag tagtttttcc aaaatggtat gcagtctgcg tgcgccgata
    12661 ttatctgtgt cacggttgac atcgaaagcg atttgagcga tccgttcaat tgcttctttt
    12721 gtaaacgtaa ctgacacatt ttctgtaccg atcaacgcga cgtattgttt tactaaagca
    12781 ttatttggtt cggtcagaat cttcacaaaa tcttcagctg tcaaatcatc taattctacc
    12841 cgtatcggga agcgtccttg aagttctgga attagatcac ttggtttaga caaatggaaa
    12901 gctcctgaag caatgaatag gacgtgatct gtttggatga tcccgtattt tgtgtttact
    12961 tgtgaacctt cgacaattgg caatatgtct ctttggacgc cttctctgga tacttctcca
    13021 gattgctgtg atttagaagt gatcttatcg atttcatcga taaaaataat tccactttct
    13081 tccgctaatt tgattgcctc actatggata tcagcatcct tgactaattt tgctgattct
    13141 tccttgatca ataactctaa agcttctttg actgttaccg tccgttccac ttttttcttc
    13201 ggtgtcaaag cacctagagt ttcatttagg tcgatcccca tttgctccaa tccattgttc
    13261 atcattggtg caggtttctt ttggtcttca acttcaatgg ttacttcacg actatccagt
    13321 aaaccctttt gtagttgttc caagactgta cggcgattga ttcgaatatc ttctgtcact
    13381 tcttcttgcg gttcagcggt attttgctga ctagcattaa aaagattcat catttgttca
    13441 aattgattag tggattgttt ctgttctttt ttgataccag gtaccaagat ttttaccaaa
    13501 cgtttttctg catttttttt agcacttgtg tatacacggc tgtactgctc tttttctacg
    13561 atttggatcg cattttctac taaatcccga accattgatt ctacatctct tccgacataa
    13621 ccgacttcag taaattttgt tgcttcgact ttgacaaatg gtgcattgac gatttttgcc
    13681 aaacgtcgtg cgatttctgt tttaccgacc cctgtaggtc cgatcatcag catatttttt
    13741 ggtgtaatat cttgttgcat tttttcatct aactgcaaac ggcgatagcg gttacgcaaa
    13801 gcaacggcaa cagatttttt tgctgcgtgc tgtcctacga tatattcatc taattcactt
    13861 acgatttgtc ttggtgtttg ttttaattca tacatatcta ctcacctctt acagttcttc
    13921 tacgataata ttatgattag taaagataca gatatctgcg gcaatattta acgcattttc
    13981 tgcaatttct ttcgcactca tgtcgttttg agcatagttt ttcattgctc gggctgcagc
    14041 caacgcaaaa ttccctcctg atccgatagc taagatacca tcatctggtg tgataacttc
    14101 tcctgtacct gaaacaagaa gcatttcttt atcattcatt acgatcagca tcgcttcaag
    14161 tttttgcata gattgctgtg tccgccattc ttgagctaat tcgacagctg cgcgttgtag
    14221 attcccgtta tattcgttta gtttgccttc aaatttttct tctaaagtaa acgcatctgc
    14281 aacacttccg gcgaaaccta caacaacttc tccattgtag atacgtcgta ctttctttgc
    14341 agtgcctttc atgacaacag attcacccat tgttacttgg ccatctccag ccatagcaaa
    14401 tttcccgtct ttttcgacgg cacagatagt ggttgaatga aatggtgatt cgaccatgtt
    14461 tgtttcctcc taaatttgtt cttttcctta tttttacgct cttggatgga aagaacgata
    14521 gtttttttgt aaactttctt ttgttacatg ggcatagatc tgggtagtcg ataaattggc
    14581 atgtcctagc aattcttgga ctgtccgcaa atctgcccca ttatttaata aatgagtagc
    14641 aaatgtatgg cgcagcatgt gaggatggat atcactatcc aaactgcttt ttttgatcat
    14701 ttgattcaag acatattcaa ttccagtagg cgtgattggt tttccatgat gattcacaaa
    14761 aacgatatca tgctcttcgt gatattttgt catcaatacc tggcggcctt ccctgaaata
    14821 ttcttgcaga gcatcttgag caaaagaacc tagaggagca tatctctctt tgttcccttt
    14881 accatggatc aacatgacct ctgaatcaaa atcaatgtct gataccgaca aactgctaca
    14941 ctcacttaaa cggatgcctg acccgtaaat gacttccagt aaagcgcggt tcctcaaatc
    15001 caaaggacta tctcctgcta cgctgtcaaa aagtgcttgc atttcatttt cataaaaaaa
    15061 acgtggcagt ctcaaatttt tctttttgag atgaacgtaa gaaaatggat tttcttttat
    15121 cacttcttct ttgagaagat actgataaaa cgaacgtagg ctggctatct tgcgactgat
    15181 agaatttctg ctatagctag gacgatgatc agctaatgca ctcaaataga cacgaaccgt
    15241 aaaatattcc gccttcagca gatcagcgtc tccagattcc tccaaaaaat gtttgaaatg
    15301 gagaatgtct tcttcatatg cttctttcgt tttttctgaa tatcctcttt cgacaatcaa
    15361 ataacgcaaa aatttttgtt cccacgtggc ttctgtcatt ttctcacctt ccttttgtcc
    15421 cagagaataa tttagcacag cgtttataaa aagacaaata aattgtcaga atttaaccaa
    15481 tatttctaag tttgttcaca attggcgcaa ttcctcacat tttgtttgag aaattcttaa
    15541 gaaaaacgca agaaaaagag cgagactatc cgtctcaccc tcttcatgga gtttccttac
    15601 tcatttggga ttgcagtttt ttctaattct gtttttattg cctcattagc ttttaaagct
    15661 cgttgtgcaa ttgcttcata tcttgctttt ttgtctcgaa tgcgctctgg cagttcagga
    15721 aacagaccat agttggcatt catcggttgg aaatgttttc cttccgcatg agtaatgtag
    15781 taagccatac ttccaatagc tgtttcttgt ggaaactcga ctggttccat ttcttttgct
    15841 aagcgtgcgg cgttgattcc agcaagtagt ccgctcgctg cactttcaac atagccttct
    15901 acaccagtca tctgacctgc aaagaacaga tcttcgcgtt tcgttgattg gtaagtcggt
    15961 ttcagcaatt ctggcgaatt cataaagctg tttcggtgca tcacaccata gcgaacgaat
    16021 tcagcatttt ctaacccagg aatcatccgg aaaacacgtt tttgctcgcc ccatttcaag
    16081 tgagtctgaa agcccacgat gttgtataaa gaagctgcag cattgtcttg acgcagctgg
    16141 ataacagcat aaggacgttt gccagtttta ggatcttcca atccaactgg tttcataggt
    16201 ccgaaaagca tcgttttgaa tcctcgctgt gccattactt caattggcat acatccttca
    16261 aaatattttt ctttctcaaa agatttaagt tctgccactt cggcattgat tagtgcctca
    16321 tggaaagctt tgaattcctc ttctgtcatc ggacagttca agtaagctgc ttctcctttg
    16381 tcatagcggg acttcaaata tactttatcc atatcgatcg tagctttgtc tacgattggt
    16441 gcagctgcat catagaaata aaaaccatct gaaccattga atgctttgat gctttctgct
    16501 aatggatgag aagtcaaagg tcctgttgct actacggtga ttccttccgg tatttcagta
    16561 atttcttcgt ttatcactgt tactaatgga tgctctttga cttttcgggt aattgtttca
    16621 gaaaaagaat cgcggtcaac agctaaggca ccaccagctg gcactgctgt ttcatctgcc
    16681 gaagtgatta ctaccgaatt caatcggcgc atttcttctt tcaataaccc tactgcatta
    16741 gccaaactgt ttcctcttaa tgaattggaa catactagtt cagcaaaatt acttgtgtga
    16801 tgcgcctcag tcgatttgac tggacgcatt tcatataaac ggacagggac tcctgcttgt
    16861 gcgatctgcc aagcagcttc acttccggca agccctgctc caacaacatt taccattttc
    16921 aatgaaagaa cctcttttct attatttttg gatgtcttct tcgtaatctc catttacaca
    16981 gactacttgt ttaccgcctt tgactttctt ctcgaccaga tatccgtcac attttggaca
    17041 atttctgccc actggtttat cccaagaagt gaattcacat tctgggaaac gggaacaacc
    17101 atagaatagt ctgtttttct tagattttcg ttcaatgact tgtccttcat gacatactgg
    17161 gcaagtcaca ccgatttctt ttacgattgg tttcgtattc cgacaatcag gaaaattact
    17221 gcaagcataa aacttcccat atttccctaa ttttatcacc atcggatgtc cacagacatc
    17281 acaatcgaat cctgctggtt catctttgat ttgaattttt tcgatttttt cttccgcatt
    17341 cgtcagttct ttttcaaacg gtttatagaa acgatcaacc actttgaccc acgcttccgt
    17401 gccttcttca attttatcca aatctccttc catttctgct gtgaaatgga tatcgacgat
    17461 ttgagggaag aattcacaga ttaatgaatt gacaatttct cctagttcag tcggctcaaa
    17521 tcttttttgt gtcaatttta catagtagcg tctttggatc gtatcaagtg ttggtgcata
    17581 agtggaaggt cgccctaccc cgttttcttc tagtgcacga atcaacgtag cctcactaaa
    17641 acgtgctggt ggttgagtaa aatgttgttt tggttcaata tcaacagatt ggactacatc
    17701 gccttcttca agttctggaa gaatattttc tttctcttct ttgccgtcat ctctaccttc
    17761 cacatacact tgcatgaatc ctttgaattt gatttttgaa ccgttcgcaa tgaagatgac
    17821 accattttgt tctaatgtca ctttcattgt atctaaaata gctggtgtca tctgactagc
    17881 cacaaaacgt gaccagatca atgtataaag tttcaattga tctttatcta aatattgttt
    17941 gagttcatct ggtgtacgaa gcacactcgt tggacgcaca gcttcatggg catcttgtgc
    18001 gccttgagca tttttgatct ttcggtgatc atgtctagaa aattcttttc catacgtatc
    18061 ttcaataaat gctgcggcct ctgcttttgc tgtatcggca atccgggtag aatcagtacg
    18121 catgtatgta atcaagccga ctgttccttg tttacccaaa gtgattcctt catataattg
    18181 ctgtgctacc atcatggttt ttcttgttcg aaagtttaac ttacgggcag cttcttgttg
    18241 caggctactt gtagtgaaag gattcgcagg gtttcttttg cgctctttct tttctacttt
    18301 tttaacttcg aagtctgttc catcaattct agcagtgact tcttttacac tttgtgcatc
    18361 tggtaatttc tttttcttac cgtcgattcc ccagaaattt gctttgaatt tcttgcgagc
    18421 ttttttaaaa ttaccgtcga tgctccaata ttcttctgga acaaattctc ggatctcttt
    18481 ttcacggtca ataataattt ttaaagcgac agattggaca cgtcctgcac ttaacccttt
    18541 cttgacctta cgccagagaa taggactgat cgaataacca acgattctgt ctaaggtacg
    18601 acgagcttgc tgtgcatcta ctaaatctac atcgatcgtt cttggttcct taaaagctgc
    18661 tttgactgcg tcttttgtga tttcattgaa gaccacccga tttttttctt tcagatccaa
    18721 cccaagtagg tacgctaaat gccaagcaat cgcttctcct tctctatccg ggtcacttgc
    18781 caagtaaact ttttgagctt tttttgctgc ggctttcagg cttttgatga catcgccttt
    18841 tccgcgtata gaaatataat ggggttcata attgttttca aaatcgatac ccattttact
    18901 tttcggtaaa tctcttatat ggccaacact ggccattact ttatagtttc gtccaagata
    18961 tttctcaatt gtcttggctt ttgcaggtga ttctacaatc actaaatatt tatacgccat
    19021 tactgaatgg actcctttcc attgtcgtgt tcctactatt aaaagaggat tcatacaaat
    19081 cgtagctaat catatccatt cagaaaacgt ttgtcaagaa aagcaaatga aaagtatggt
    19141 gttattcttt aaattctgcc aagatatctt cacccgaaac cgtgcaaact gcaccgtctt
    19201 ggatcaaatg atgacatccg gtagaatgtt ctgttaaaat atttcctgga acagaaaaaa
    19261 ctgttcttcc aagatccaat gcttgctgtg ctgttatcag agagccgctt tttttcttgg
    19321 cttcaaagac acatgttcct ttagacaaag ctgcgataat ccgatttctc attggaaaac
    19381 aatgcttttt cgggccggat tgaggaggat attcactcaa aagaagatga tcatttgcaa
    19441 tcacctcttg caagtcacga ttttcagcag gatagacttg ctctatccct gttccaatga
    19501 ctgcgattgt ccgtccacca tgagagattg ctgcttggtg accgtatgta tctatccctt
    19561 tcgcgcagcc actgacgatc acatatccag cttctatcac tggttttagt aaataataaa
    19621 ctgttttttt ggctaaagca gaagtgtgtc ttccaccaac aacgcttaac atgttacaag
    19681 aaagtaaaga tagatcacca ttataaaaca gtacaattgg cggataggtc aagtgaagca
    19741 gctgaggggg atatttggga tcaaagcaag tgataaacga ctgtttcgat tggattttgc
    19801 cccagttagc gtgaatttcc tgccaacttt tgcgaaacag ctcccggtgt ttggttattt
    19861 tacctatctt aatgatgtct tcaaatccga tttctttata aggatactgc atcatccagt
    19921 tagcaagtgc ccattttcct tgactactga ttccttttgt ataagcaagt ttcaataaat
    19981 tttcttctat ttgatacatc ataaaaccgc ctttcgtctc tctttctaat aagatacgta
    20041 aaagcggctg ttttttattg taaatatttt tttacaggtg caaaactttt acgatggatt
    20101 ggcgtaattc cttgtcgttc aagtccttct aagtgttcct ttgttccata acctgcatta
    20161 tgcgagaaac catatccagg ataaagcacg tcatattctt tcatcaattg gtctcgatat
    20221 actttagcaa tgatacttgc agcagcaatc gacacagatc gtgcatcccc tttgattatt
    20281 ttttcctgct gaataggcaa atccaaggtc atcgcatcga tcaacaaata gcttggtaca
    20341 atggacaatt tttctactgc ttggatcatc gctaatttac ttgcttcata aatattcaca
    20401 tcatcgattg tctgctgatc aacgatcccg atcccgattg ccagggcttt ttcttgaact
    20461 tgttgataaa gaagttctcg ctttttctct gataattgtt tggagtcatt cagccctaat
    20521 atgatctcat ttttcggcaa gataactgca gctgccacga caggtccagc taatggtcct
    20581 cttcctactt catcaatgcc ggcaatccat tggtgacctt gtgcgtaccc ttgcttttca
    20641 aagcaaaaca tttcttcata tttttcctta attaatgctt cctttttcaa tcgtcgttca
    20701 aaagaagcca gtgcttgctg cacgcctttg cgtggatcat ttcttaatga ttctagtgta
    20761 ggatcatcta ttctctgtat gcttttcaat cgttctttga tactttggat ggattcagtc
    20821 atcacttttt tgctccattt cttcgcaacg atccagtgtg tatcgtccaa gttttccttg
    20881 acgaatatca tgcacgacta attcgctgcc acgatcatag tcttctttat acccacgttt
    20941 tgctgtaata tccatcagta attccggtgc caatttttct tcttgctcga ttgataaacc
    21001 ataacgatcg ctgattcttc ctgaataata tcgggcaaaa aagtctaatc cgtaaatcgc
    21061 taaatcatct aaatggacaa gctggtcttt gatcgcgcct gtcagcgcta gttttttgcc
    21121 gatttcttca tcttcaaatt tgggccataa aatccctggt gtgtctaaca attccaactc
    21181 attacctaaa cgaagccact gctgaccttt tgttacgcca ggcttatttc ctgtctgtgc
    21241 aattttcttg cctactagtc gattcatcaa cgtagatttc ccaacatttg gaatacctaa
    21301 gaccattgcc cgaatagcgc gaggcttgat cccccgagca gcatctcttg cccgtttttc
    21361 tttcaatgct tctttagccg caggtactag acctttaatt cctttacttt cttgtgccgt
    21421 gatagctaat gcaagatggc cttgtttacg aaaatattct tgccacagct ttgtctgttg
    21481 tggatctgcc agatccgctt tattcaataa aacaaggcga ggtttttgct gaatgatctg
    21541 atctagcatt ggattacgag aagataaagg taatcgtgcg tctaccaatt caaatacgat
    21601 gtccacatat ttcatttttt cagaaacttc tcgtcgggct tttgccatat gtcccggaaa
    21661 ccattggatg gtcattgttc tctccattcc tttcataccg gaatattccc cggctttact
    21721 tgtttttttc tttttcaata taaattcact tttttattat aaagaaattc catctattag
    21781 ttaagtgaaa gtgaaccagt cctcattcta tcacatcttc acttttttga tgatgcaaat
    21841 ttttattttg gtttctatat atgaatctaa atttttgaca gcgtttccta aataagagat
    21901 gatagacgaa caaaacagaa aggagacgaa acaaatgaat agtatggttt ttgttaattt
    21961 tcctgttgct gatgtcaatc gttccgttgc tttttatgaa aagctgggtt ttaaaaaaaa
    22021 tgaagaattt tctactgaag aagctggttc aatggtatgg gatgacactt tttggataat
    22081 gcttctctct catgattttt ataaaaaatt tataaaagat aaagaaatag ctgataataa
    22141 aaaaacaagt ggtgctttga tttcgtttag tgttgaaaat gcggatgaag taaaaaaatt
    22201 tgctcaaatt gcaaaagaaa atggtggtac tttccacgaa gtagatatgg gaatgcctga
    22261 agaagaaatg ttttcattag aagtccaaga tctagacgga aatacattag aacccgtatg
    22321 gatgaagatg taagcagata actaatctga cgttttttat aaaaaaaaaa caaaggcgga
    22381 tgatacgata agtattctcc gcctctattt tttttattcg tacaatctct tttttacagt
    22441 ttataaaagc agtcttctca cccttttttt aattgttaga tgccagtgta tgtaaatcac
    22501 caatgtgtca tgacacattg gtgattttat ggtatccttt tattattaac tgacaaagga
    22561 gagaatattt tgaacacttt gattgcaaaa gacgttttta gattgcccga aatgaatgca
    22621 agattgttgt cgacctctgc tcttttatta gcccttgatg tcatcttata caagatagcg
    22681 attggtcctg cttattttca aatcacattc ggatttttaa gtatggcgct tatgggttat
    22741 ctatatggac cgatctgggc tggtttgctt gaaatgttgt cgaacacttt aaatttcacg
    22801 atatttggtt caggaacgtt tcaagctgct tttcttatca cggctttcct tggtggattt
    22861 attaatggct tatttttata tcataaaaag ataaacctgc cttccgtcat cgctgcccaa
    22921 ttattgatta tgatcccagt cagccttttt atcaattctt ggctgatgtc tgttttgttt
    22981 ggtgcagatt tcaaagtaat ttttctaggt agattcatca ggaatatgat ccaaatccct
    23041 attcaaataa tggttgttta ttcatttatc aaaactttag aggctagaaa agtgtttaaa
    23101 cgagctgttt gattttacgt actaaggtag taaactgatt ttggattaaa caataaagcg
    23161 tgcagtctca acagactgca cgctttattg tttctcactg gatccatttt atttcatcaa
    23221 gtggataata gacaaatctt gctttaccta gaatatcagt tttattgatc gtgccaaacg
    23281 aacgactatc ttttgaaact cttcgattat ctccaagtac aaaataatta tcttccggaa
    23341 gcttggattg tcccgttagc tcttgtagtg taaagtccgt cgtataggat gcatttgcat
    23401 gatctgattt caggttttta gttagataag gttccttaac tactttccca ttgatcttca
    23461 attgatcatt ttggtagctg acattttctc ctggaagacc gattactcgt ttgatataaa
    23521 tcgtgccatc tgctaattga aaaacaacaa tatcaaatcg tctgatctcc gaaaattttt
    23581 ccatcacaac catatcacct tgcttcaaca cgttttccat tgaattgcct tccactggta
    23641 ccgggatgaa taaaaatccc cgcaacatga acgcaaggaa tacagaaaca agtaagtatt
    23701 tgaatacaag ccagaagcga ttaatatagc gttgtttttt tgtcaatctc tatcaatcca
    23761 tccctctttt tttcattctt tctttattat aagaaaaata tcaaaaaaat gcgactagat
    23821 agctgaaata aaaataattc tttcaattag gctattggac aaaaaacttt tttgatcata
    23881 aagtacaaaa ctatctctta tatgtcatga ttattttcta aatatttttc ccatgcttcg
    23941 tcaaagatat ccatggacgg caaatagtcg acattcagtt ccagataatt agaaatttct
    24001 tcgtagctag agctgtgctt aggaaattgg atgtctttac cggcttctgt agcgaaaaca
    24061 gataaagcat catgtgtgcc accgcctcgc agtgtcatta aataatgaaa aaagcttcga
    24121 ttcacccttc gttcctccaa ttttcttcga taaaggcagc ccggcgtgca tggtttcttt
    24181 taaaattttc aggatgcttt ttataatact cttgatggtc ttcttctgct agataaaatg
    24241 gttgggctgg ttcgattttt gtaacgattg gctgttcgaa acgtccactt tcagctaact
    24301 gtttcttact tttttcagca atgatccgct gttcttctgt caaataaaag atcaccgggc
    24361 gataattgtc accgcgatcc tcgaattgtc cgaaagcgtc agttggatca gtttgctgcc
    24421 agtaaagttc tactaattgt tcatatgaaa tcaatgttgg atcatagata atctccactg
    24481 cttctgtatg tccagtcgtt tggcttttta cttgttcata agtcggatct ggtacatgcc
    24541 ctccagtata tcctgacatc actgtatgga taccaggaag cgtgtcgaaa ggctggatca
    24601 tacaccagaa gcagcctcct gcaaatattg ctcgttccat tatgattccc cctctaatac
    24661 tactcatccg atgatttttt attttctttt gtcttgcttt cagtactgct ggtgctagtt
    24721 ttgttggtat ttgagttgtt tgaagcatca gaggtatctt ttggcaaata tagactaaac
    24781 cgaatgtcat catttactaa gtcgatttta tctgctttaa tatacatccc gttttgcatt
    24841 cggaattgat ccaacctaag catgatagtc agatcatctg tattgacttc gatccaatct
    24901 ggtaatttat agtttctctt gatcatattc atcacatctt tgattggcaa actcaatgtt
    24961 ccgatagaaa gacttctagc ttttagttgg acatttccgt tttccatcac atacggatca
    25021 aaatataaat agaaatcgat aggaaaacct agtacttcga aagtaccatt gagtagtgct
    25081 tcattttcca aataaaattt atattcaata tcactatctt tttgatactc actaaggaaa
    25141 aagctgatga tttgattgac tttttcttta tttgaattga tcgttaagac aggatctcct
    25201 tgtctttccg taagagctgg tatttctggg agttctggtt cccgattagc aaaaatacgg
    25261 ctgcctaaaa atacggtcga accaagtact accccaagaa ggataaggaa tgcgaatttc
    25321 cagtaatttg ttttctttgt tttgtttgaa gatacgtcag atcttgtttt catgtttatt
    25381 gatcatcctc cttctctaac cattggtttt gtgtctcgat cagctgatct ctcactgcat
    25441 ttgccatcaa ttgataacct aaattatttg gatggaaatg atcttcttca tagagcacat
    25501 tatttttgat gccatttaga ttttctgtcg tgctagttgt ttgatctgtt ccttcttctg
    25561 tgacacctac ttcctgattc aatccttgat aaagcaaatc attgattggg ataaagtagc
    25621 agttcggcat tttttttgtc accgtttctg ttcctttgtt ccaattgtta acaattgtct
    25681 gcatgtcagt gatctcagga aagttcaaat aaaatggatt gtagatgcct aaaatgtaaa
    25741 taggtgcggt tttattgagg gatcgaaatt cttccaatag ttctgtgaca ttttcctgat
    25801 agtttttcag tggcttttta aaggaattga ccgttaaccc gaataaatta ttttggataa
    25861 ctttcatcaa atcattaccg ccaacagtca gtgtgataaa atcagctgtt ttgatattgt
    25921 ttcgaatttc ttcttttgtt tttactcgct ttaagatctg gtcacttcgt tcaccattta
    25981 caccatagtt ttctgtttca accgcggtca aattataacg gtcttttaga tcatttgcga
    26041 caaggggaac gaatcctcct tgcttggttt gatctcctac tccttcagtc agcgaatctc
    26101 cgatagctat gtagtgaagt ttttcttttt tatttggatc ttctctcaca gtagttactg
    26161 gtttcattag tgggccagct ttcggtatag aagcagctaa acctgcatag atgatgatcg
    26221 caattaataa tgggaacaaa acgaggatca aatgtcgttt cgttaatttc attttttcac
    26281 ctcttgtatt cgattggttc taatgttatc aaaaatttta tcaaaaaggt agtctttatg
    26341 aaaaaagaaa acaattattt tcctaatttt ataaagaaaa agagaataag ctgcaagcct
    26401 ttcaggctga gggcttatcc tcttttcgac taatctacat aatacatgat tgcaaacgcg
    26461 tctttaccag tatgcgtagc gataaccgga ttcgtatgca gtacagggat atgcatgtcc
    26521 gggaataatt cttgcaaacc ctctttgaat tcttgcgcca attcttttgc atccgcatgt
    26581 gaaataccga tttgtcgcac atttggcaat tctttcagtt catttttcaa attattaaac
    26641 catttatcga acgttttctt tcctcgcccc ttagttacag ggattaattc agaatgggcg
    26701 aaattcatca ccacgcgtat gttcaaaaca ctagacagca aaccagttgt tcggctgatt
    26761 cttccacctt ttaccaagtt atctagtgtg gatattccga tgtataattt tgtattgtct
    26821 tgtacttttt tgacttcttc aaggattttc tctactgttt taccttcttt tgctaattca
    26881 gctgctcgaa ttacctgaaa agataatcct tggtcagtgg tatcactgtc gataaccgtt
    26941 actttcgttt ttgttagatt actggcttgt cttgctgctt ctactgttcc actcaagcct
    27001 tttgtcatat ggatcgaaat cacttcactg ccgtcttctc ctagctcatc atacatcgag
    27061 atgaattctc caattggtgg ttggcttgtt ttgggcagat tttttgcttt cgccatcatt
    27121 tccataaatc tttcgccgtc tagttggtca tcatcgggat atactgttcc gtcgatcatg
    27181 atggatagcg gcatgacacg aatgcctaaa gcatcgcgta catttttttc catcgtacat
    27241 gaagaatctg ttacgatttt tacatttgtc attattttct ctctctcttt ctttaacaag
    27301 atgccttatt catgttaaac tacatgtaag acaatttcta tttttagtat aacagacaaa
    27361 taatgatttt tcattaatta cacctagtaa aggagccgtt ttcatggaaa aaacgaaatt
    27421 taatcgaacg tatgaaatat taaatgaagt gtttaatgca gtcacccatg gaattgggac
    27481 tggattgagt atcgcgggac tagtggttct tttagtaaaa ggtgcccact tgcattcctc
    27541 cctccatatt gtctcttatg ctatctatgg atcgatgatg attcttttat ttttaacgtc
    27601 tactttgttt cattcattga tttttacaaa agcaaaaaag gtgttccaag tattcgatca
    27661 tagttcgatt tttcttttga ttgcaggaag ttatactcct ttttgtttgc tgagtatccg
    27721 aggatggcta ggttggactt tatttgtatt aatctggttg tttgcgattc ttggcattat
    27781 atataaagcg ttgacgttgc ataaacggga gagagtatca aaagtatcta cgatcatcta
    27841 tatttttatg gggtggctct gtgtcgtggc tgctgttcct ctttatcact ctcttggaat
    27901 gactggtgtt attttgatgg tagcaggcgg gctttcttac acgataggcg cattctttta
    27961 tagcttgaat tctatccgct atatgcatgt tgtttggcat ttgtttgtta tgttaggtgc
    28021 aggttttatg tacttctctg ttttattctt tacttaataa aaaaaataaa gctatggatg
    28081 ggacagctct aaataacagc tagtctatcc atagcttttt tatatcgtca attcgcctgt
    28141 cccgcttttt acaggattct cattacgctg atacgtttca aattgatgcg gataacgatt
    28201 tttttcatcc atttctcctt gactagtatt gatcagtgac caatcagacc agtcaacagg
    28261 tggaaaataa gcatctccat cgaatacatg atggatgact gtacggtata atatcgtaca
    28321 aaatggcaga aattcttgat aaacagctga ccctccagcg atgaaaaaga taccgtctgt
    28381 ttctttttcc agagcaagca cgtcttccac actatgcatg acatgcacgc cttctgcttt
    28441 ataggaagaa tctctcgtca aaacaatcgt ctgacgattc ggcaacggac ggctacccat
    28501 cccttcgaat gtttttcgtc ccatcactag tgtatttgct tctgtcattt gtttgaagaa
    28561 tttcagatca tttggcaaac gccaaggaag acggtcttct tttccaatca atccgttttc
    28621 atcctgtgcc caaattgcag ctaacatttt ctcatccttt ctgattctaa acagcgatag
    28681 gagctttgat cgccggatgt gggtcatatc cttctaattg gatatcttcc atctcaaaat
    28741 caaatactga ctgcttttcc tgattcaaaa ctaaagtcgg aaatgcgcgt atttctcggc
    28801 tcaattgttc tttcatttgt tcgatatgat tgatataaag atgagcatct cctaatgtgt
    28861 gaacaaattc gccaaccgct aaacctgttt catgtgcaat taggtgagtc agcaacgcat
    28921 aacttgcaat gttgaacgga acgcctagaa acacatcccc actgcgttga tacaattggc
    28981 agcttagccg tccctcttga acataaaatt gaaacatcgt atgacaagga ggcaaagcca
    29041 tgctaggaac atcttccgga ttccatgcag aaacgatcaa tcttctagaa tcaggagtct
    29101 ttttgattgc ttcgatcact tgtttgattt gatcgataaa gccaccgtct tttgtttccc
    29161 agtgacgcca ttgatagccg tagatgtgtc caagatcgcc gtatgtttca gcaaaggaat
    29221 catcggttag gatcttctcg caaaaaagct tgctctgctt tttatattct tcattgaatt
    29281 caggatctgt caaacatcta tggccaaaat cagtcatatc tggtccatta tagtctggac
    29341 ttttgatgta gcgttcaaat gcccattcat cccaaatatg gtttttattt tgtaatagat
    29401 agcggatatt cgtatctcct tttaaaaacc aaagcaattc gcttttgatc aatccaaaag
    29461 gtactcgttt cgttgttaac aaaggaaacc ctttttgcag atcaaaccgc atttggtatc
    29521 cgaataagct taatgtgcct gtgcctgttc tgtcttcttt acgatgccct tcttccaaca
    29581 attttcttcc taagtcaaga tacgcttgtt ccatcctttt tcctcctaat cttctaccca
    29641 ttcactcaag tattcccacc gtgccatttt ttcttcaagt tgtgcttctg tttcagatac
    29701 gtcgttttgc aattcttgta atcgagtgaa attatctcct tgatgattca tctcttcttg
    29761 gagcaatgag attttttctt caagttctgc aatctcatct tcaatcgttt cccattcttt
    29821 ttgttccatg taagataatt tcttcttttc agcaggttcc ttgctctgta tttttggttt
    29881 cgctttttca gcaggctgct gggtctcttt ttgcttcagt aaataactgg acatgtttcc
    29941 gtaataaagt tcttgttttc cgtttccttg gaatactaat aacttatctg ctaccttgtc
    30001 caagaaataa cggtcatggg aaacagtgat cacagcacct ggaaaacttc taaaataatc
    30061 ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc ggttcatcca ataataaaac
    30121 attaggttgc tgaatcagca gtttcaacaa aaataaccgg cgtttttctc cgcctgaaag
    30181 tttgcctatc agtgtcccat gcataaatct tggaaataaa aatcgttcaa gtaattctgc
    30241 tactccgatt tgactgccat ctgctgtttt tgcttcttca gcagcttctt gtaagtaagc
    30301 aatcatccgt ttatctggtg ccatttcttc attttcttgt gtataatacg caagatgaac
    30361 cgtttcaccg atagacattg taccgctatc caggggaatt ctgccagcta gaatattaag
    30421 caatgtcgat tttcccgcac catttttacc agtaatacct agtctttccc gagattgaat
    30481 caataaatct aaatgttcta gcagtgtttg gtcatttata cggtaactgc catctttgat
    30541 ttccagtact tttttcccta aacgttgtgt cgctaggttc agctctaaat catcttcttg
    30601 gtttacttgg tacaagttct cctttagatc ttcgaaacgg ttgatccgtg cttgctgctt
    30661 ggtgccacgt gcctgtacgc cagccctcat ccaagctaat tcctgtttat aaagccgctt
    30721 gcgcttttct tcttgttcta cttctacacg atcacgttct gctttttcta agacgtaagc
    30781 ttcataattt cctttatact cgtataattt tccaaatgaa agttcgaaga ttcggtttgc
    30841 tactctatct agaaaataac gatcgtgggt aaccatcaat agtgcgccac ggtattgttt
    30901 caaatagttt tccaaccact cgatcgcttc ataatctaaa tggtttgttg gttcgtctag
    30961 taatagtaaa tcgggttcat caatcaacac ttgcgccaga ctaactcgtt ttttctgacc
    31021 acctgataaa gtactgattt ttttatctaa ttcactgatt cctaattttt gcaaaataat
    31081 ctttgcgttg gtatctgtag tccaagcatc ttctttattc atttgttctt ctgctttagc
    31141 ataacgtttt tgcacatcag ggtcttctcc gttttcccct agttcgatca atgcttcctc
    31201 atatgctcga acagtttgga tcaaggggct gtttccttga aagactgctt ggagaacagt
    31261 taattcatcg gaaaattcag ctgcttgaga aagataacca atacggtaat cagttggata
    31321 aaacactgtt tgccgatcac cgtcgctgct atcgataccg gcaataatat ttaagagact
    31381 tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt ggaccaaaaa
    31441 agaaatctgg tcaaacaaat ctttttcacc ataagttttt ttgaggtcag ttacttttaa
    31501 ttctttcaac atgttccctc cttatcttgg atattgtatc ataaaaatag tgaacttcag
    31561 gaaaattttt ttggtatttg ttctttttgt gttctcaaaa aaagaacagg accgattcga
    31621 tcctgttctg atatgttttg ttttttataa aacttgaaaa tttttacctt tttattttat
    31681 tggactcgtt ttttttgact ttgctcgaaa cgcaaatcgg aaagaaaagt tatcagatgg
    31741 tttgttcatc gtataagatt ctaacaaatt gatattcctg cgatagctgt tcgcagaaaa
    31801 cgtcgtgatt ttttcagcaa cttcgtattc atcaatgatt tttctttcaa gataataacc
    31861 tcttagcatt tttttcgtaa acagactatc ttgctggatc atccacgaag aaatcaaatt
    31921 ccccgagttc atttttattc cttcaagttt gcgttgctgg ataaaaaatt gaaccatcgt
    31981 ttcatcataa atatcagtca agttatctaa actttcaata atcaactgag tatttttttg
    32041 atatgtctct ctgatttctt ggatatcttc atcactaata aatgtctgac gatcagcata
    32101 tcttctttgc caaaacattt tcggatggag aacgattcgc attaacctac ggacaacaat
    32161 gaaccaaaag ccgataaaag acaaaatctg gcttgtgata gaatgttcga atcgagaaag
    32221 aaaacgggag taaaaacgat aaccattaga agaaagcttg cccgaacgat aactttcatc
    32281 caatccatcc cgttcgatcg acaaaatcag tgcttgtatt tcttgtactt cttgcttttc
    32341 atcatcggtt aaatcttcca agtacagatc acgtatccgt tcttgataat tttctatcac
    32401 tgcttcagtg gctaaaaatt cttttgtatt acgatcaatt tcctttattt cttttcgcaa
    32461 aacttcaacg acctcttcta aaatcatcaa agcattcaaa tccgtgcttt tcgcttcctc
    32521 tgtttccgtc agcataggca agacaatgat ccctatgacc aaagtcacta agatgacacc
    32581 cgccgtcaga aacaaaagca aagaccgctg gtaaaacatc atattattga ttgaaaatgg
    32641 caaaataaaa atcgtagcca aactgactgt tccctttaca ccgccaaatg ttaagatcaa
    32701 gatctcgttc aactgttttt tgaacttttt actgccatct ttgaatacat aaaaaagact
    32761 gatagaaatg aatctgatca caaaaagcat gacagagatt aatacgataa tcgctaataa
    32821 aagaccgttt ggatataaac cgtctcccca tactggcgag aacacttgcg tcaattcgat
    32881 tcctaaaaat ataaatacta atgcattcag tgtaaaagca atagttgtcc atgtactgtg
    32941 ggacaagctc gacaattctg catcaaacac agagattttc ctaaaaccag aagcttggag
    33001 aatacctgct gccactgcag caatgattcc agatacatca aatgcctctg ccaatacata
    33061 ggctagaaaa ggaagcaaaa gttcgatcag cagataccct gtgacatctt gtgctgatgc
    33121 tttttcgatc aaatgaatta ttttttgttt gaaccagaca agcaaaaagc cgatcaaagc
    33181 tcccccaata ctagaaacta ctagactcat ccccgcgttt actgctgaaa aagatccggt
    33241 gatcagcgca ccaagagcaa actggaaggc agtaacacca gatgcatcat tcaaaagacc
    33301 ttctccttcc aaaatatgca tcgccttttt agggatattc actcgtccag aaagagaact
    33361 gactgctacc gcatctgtcg gacctaacgc tgctccaaaa gcaaagcagg cagcaagtgg
    33421 gacacttggc aacaggaatg ataacgtcgc acctaccccg acaagggtca aaatgactcc
    33481 tccaaatgcc aagaacaaaa ttgtcccgaa atttttgaga atcgaagaaa tatctgcttt
    33541 ttctccttct ctaaataaca atggtgcaat aatcataact aagaacaatt caggttcaaa
    33601 atcgattgat tgtccccact ctgtcagacc taataccact ccaaggaaaa tttggatcag
    33661 tggagctgga atcattggca aaatgcggct agctatgttc gataaggtga tcgttactgc
    33721 aaatacaata actaactcag caaattccat cgtccatccc ctttattttt tattttgtgt
    33781 aagcattttt aaaaattgtt cttttttctt taaaattttg gtttcgacta cttcaacctg
    33841 tgtttggttc ccctctaatt cacactgtac acgttttgac tccaaacgaa gcagatcatg
    33901 tttcagacgt tccaactgac gatacatcaa tatatttctt ttacgcaact tacaaagtat
    33961 ttgacggcgg tcatccatcg aaagggcgcc atagcgatca tgcaacagtt ttttttgttg
    34021 ttcaaaactc tctctcattt tccagatctc ctatacacaa acttccatca ttatagcaaa
    34081 gttctttttg gatgaaaaat atttcattgt ttcatcgttt attgctagtt agaaatttcc
    34141 ataaattttt caacgcctca ttctattcca aatagtatca gatatcaagt aaaacttaag
    34201 aaagataaaa gtttctgaca aaaggatgtt tactttccca ttcctgcctt tcttgttctt
    34261 ttatcacaac aaaaaaatgg ggctgcgaca aaaat
//
LOCUS       DAXWLG010000010        33933 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXWLG010000010 DAXWLG010000000
VERSION     DAXWLG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.010"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            34..402
                     /locus_tag="ACGE4T_000360"
     CDS             34..402
                     /locus_tag="ACGE4T_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFF8385676.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            complement(605..1918)
                     /locus_tag="ACGE4T_000361"
     CDS             complement(605..1918)
                     /locus_tag="ACGE4T_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HFF8385677.1"
                     /translation="MATLHEKKVQFNSKLTISNTGGNLSTDSGLVLVKEFMESLNFSD
                     LSKQYLGIEDKRLYHIHDNFSLMEQLIYQNIAGYSTDSSANILKQDPIFKVILDKSRL
                     ASQSSISRFWDRISEENISQLQDLNQAMIDKVRSARNTTEMIFDLDSTHSDTYGDQEN
                     TDYNAHYRTNGYHPLVAFDGLTGDFLKAELRSGNVYTSNGVGTFMEPLFEHYNQVVPV
                     SNILVRGDSGFATPELYDLCEAYDSFFVIRLKANRNLSKIAESFIQIDDNHPWDKKEV
                     VYSSTSYQAKSWSKERRVCIKSTREADELLFRHEYIVTNYSNNVSAETVFRTYCKRGT
                     MENFIKEAKNGFYFDKTDSPSFLENHARMMVSLLAYNIVNFMRTLCFTQGSASMQVDT
                     IRLKLFKVAGKLVRTGRRLLLKLSSYHLHQGLFYQVLGNIQQLCW"
     gene            2236..2667
                     /gene="mraZ"
                     /locus_tag="ACGE4T_000362"
     CDS             2236..2667
                     /gene="mraZ"
                     /locus_tag="ACGE4T_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFF8385678.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2688..3647
                     /gene="rsmH"
                     /locus_tag="ACGE4T_000363"
     CDS             2688..3647
                     /gene="rsmH"
                     /locus_tag="ACGE4T_000363"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFF8385679.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3652..4053
                     /gene="ftsL"
                     /locus_tag="ACGE4T_000364"
     CDS             3652..4053
                     /gene="ftsL"
                     /locus_tag="ACGE4T_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8385680.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            4054..6246
                     /locus_tag="ACGE4T_000365"
     CDS             4054..6246
                     /locus_tag="ACGE4T_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8385681.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            6272..7234
                     /gene="mraY"
                     /locus_tag="ACGE4T_000366"
     CDS             6272..7234
                     /gene="mraY"
                     /locus_tag="ACGE4T_000366"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFF8385682.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            7248..8609
                     /gene="murD"
                     /locus_tag="ACGE4T_000367"
     CDS             7248..8609
                     /gene="murD"
                     /locus_tag="ACGE4T_000367"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFF8385683.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8635..9723
                     /gene="murG"
                     /locus_tag="ACGE4T_000368"
     CDS             8635..9723
                     /gene="murG"
                     /locus_tag="ACGE4T_000368"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFF8385684.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9738..10958
                     /locus_tag="ACGE4T_000369"
     CDS             9738..10958
                     /locus_tag="ACGE4T_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFF8385685.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            11114..12439
                     /gene="ftsA"
                     /locus_tag="ACGE4T_000370"
     CDS             11114..12439
                     /gene="ftsA"
                     /locus_tag="ACGE4T_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFF8385686.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            12462..13703
                     /gene="ftsZ"
                     /locus_tag="ACGE4T_000371"
     CDS             12462..13703
                     /gene="ftsZ"
                     /locus_tag="ACGE4T_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFF8385687.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13720..14397
                     /locus_tag="ACGE4T_000372"
     CDS             13720..14397
                     /locus_tag="ACGE4T_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFF8385688.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            14411..15010
                     /locus_tag="ACGE4T_000373"
     CDS             14411..15010
                     /locus_tag="ACGE4T_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFF8385689.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            15091..15312
                     /locus_tag="ACGE4T_000374"
     CDS             15091..15312
                     /locus_tag="ACGE4T_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFF8385690.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            15361..16143
                     /locus_tag="ACGE4T_000375"
     CDS             15361..16143
                     /locus_tag="ACGE4T_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFF8385691.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            16164..16871
                     /locus_tag="ACGE4T_000376"
     CDS             16164..16871
                     /locus_tag="ACGE4T_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFF8385692.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            17133..19931
                     /gene="ileS"
                     /locus_tag="ACGE4T_000377"
     CDS             17133..19931
                     /gene="ileS"
                     /locus_tag="ACGE4T_000377"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFF8385693.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19980..21506)
                     /gene="zwf"
                     /locus_tag="ACGE4T_000378"
     CDS             complement(19980..21506)
                     /gene="zwf"
                     /locus_tag="ACGE4T_000378"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFF8385694.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(21521..22168)
                     /locus_tag="ACGE4T_000379"
     CDS             complement(21521..22168)
                     /locus_tag="ACGE4T_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8385695.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(22352..22681)
                     /locus_tag="ACGE4T_000380"
     CDS             complement(22352..22681)
                     /locus_tag="ACGE4T_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFF8385696.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22858..23586
                     /gene="sfsA"
                     /locus_tag="ACGE4T_000381"
     CDS             22858..23586
                     /gene="sfsA"
                     /locus_tag="ACGE4T_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFF8385697.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            23602..24615
                     /locus_tag="ACGE4T_000382"
     CDS             23602..24615
                     /locus_tag="ACGE4T_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8385698.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            24615..25892
                     /locus_tag="ACGE4T_000383"
     CDS             24615..25892
                     /locus_tag="ACGE4T_000383"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8385699.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25955..28657
                     /locus_tag="ACGE4T_000384"
     CDS             25955..28657
                     /locus_tag="ACGE4T_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8385700.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28809..29126
                     /locus_tag="ACGE4T_000385"
     CDS             28809..29126
                     /locus_tag="ACGE4T_000385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8385701.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            29156..29476
                     /locus_tag="ACGE4T_000386"
     CDS             29156..29476
                     /locus_tag="ACGE4T_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8385702.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            29584..31044
                     /locus_tag="ACGE4T_000387"
     CDS             29584..31044
                     /locus_tag="ACGE4T_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8385703.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            31112..31333
                     /locus_tag="ACGE4T_000388"
     CDS             31112..31333
                     /locus_tag="ACGE4T_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385704.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            31364..31546
                     /locus_tag="ACGE4T_000389"
     CDS             31364..31546
                     /locus_tag="ACGE4T_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFF8385705.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            31546..31959
                     /locus_tag="ACGE4T_000390"
     CDS             31546..31959
                     /locus_tag="ACGE4T_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFF8385706.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            32082..33263
                     /locus_tag="ACGE4T_000391"
     CDS             32082..33263
                     /locus_tag="ACGE4T_000391"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8385707.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(33334..33507)
                     /locus_tag="ACGE4T_000392"
     CDS             complement(33334..33507)
                     /locus_tag="ACGE4T_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385708.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTPKTKE
                     NKKFKFTKKEDLL"
     gene            33661..33734
                     /locus_tag="ACGE4T_000393"
     tRNA            33661..33734
                     /locus_tag="ACGE4T_000393"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33695..33697,aa:Arg,seq:cct)
BASE COUNT        11284 a         5855 c         7210 g         9584 t
ORIGIN      
        1 cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg
       61 ttttggtata tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt
      121 tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc
      181 ggtatgcatg cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt
      241 tctgttttgc taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt
      301 atttatccgc gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg
      361 tacgctgtct tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaacctgaa
      421 taattcatac cgataaaaaa atgatctgtt gctctaaatt ttaccggaat ttatattttt
      481 tattccgtct cagaacccaa cagattaaaa aatccgaact gtttaggtaa acagctgcgg
      541 ttcagccaaa tttgggcgca ctcgtccctt gaagtacaat ataattttta aaattgggat
      601 ttatttacca gcagagctgc tggatatttc cgaggacttg ataaaataag ccctgatgta
      661 aatgataaga actgagtttc agcagcagtc tacgtcctgt tcgaacgagt ttacctgcga
      721 ccttgaatag ttttaatcgg attgtgtcca cctgcatgct ggccgatccc tgtgtgaaac
      781 aaagcgtacg cataaagtta acgatgttgt aagccaacag gcttaccatc atgcgagcgt
      841 gattttctaa aaaggaaggg ctatcggtct tatcgaaata aaagccattc ttcgcttctt
      901 taatgaagtt ttccatggtg ccgcgtttac agtaagtccg aaagactgtt tccgcagaga
      961 cattattgga gtaattggtg acgatgtatt catgtcggaa tagaagttca tcagcttccc
     1021 gtgttgattt aatgcagact cggcgttctt tggaccaact ttttgcttga taggatgttg
     1081 aagaataaac aacttctttt ttatcccaag ggtggttatc gtcaatctga ataaagcttt
     1141 ccgcaatttt tgaaaggtta cggttcgcct ttaagcggat cacaaagaag ctgtcataag
     1201 cttcacagag atcgtaaagt tccggtgtag cgaacccgct atctccacgg acgagaatat
     1261 tgctgacagg aaccacttgg ttataatgct caaaaagagg ctccataaat gtcccaacgc
     1321 cattagacgt gtagacattg ccagaacgaa gttctgcttt taagaaatct cccgtcaaac
     1381 catcaaatgc gactaacgga tggtatccat tggttcgata atgagcattg taatcagtgt
     1441 tttcttgatc tccgtaagta tcagaatggg ttgaatccaa gtcaaagatc atttctgtcg
     1501 tatttcttgc cgaccgtact ttatcaatca tggcttggtt aagatcttgc aactgagaaa
     1561 tattttcttc gcttatgcga tcccagaatc gggaaatcga agactgcgag gcaagcctgg
     1621 acttatccaa aatcaccttg aatatagggt cttgcttcaa tatatttgcg gaggaatcgg
     1681 tcgaatagcc agcgatattt tgataaatca actgctccat caaagaaaaa ttgtcatgga
     1741 tatgatagag acgtttgtcc tctattccca agtattgctt agaaagatcg gagaaattaa
     1801 gagactccat aaattctttg acgagaacca atccagaatc ggtggacaaa tttccacctg
     1861 tgtttgaaat cgtcagttta gagttgaatt gtaccttttt ttcgtgtaat gtagccattg
     1921 agagagcccc tttctatggt tttagtgtgg taacttaacc atatcagatt ggggttcttt
     1981 ttgtacaccc aaagggtgct tatcgaacaa gtgaaaaatg ctattgcagc aaggttattt
     2041 gtagtttcct gaaattttat gaattattca ggtagaaaag ctgtaaaaag tcttttaaaa
     2101 ggctttttgc agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat
     2161 ttcccagaaa aggtggtaga aagtggggga ttgtggtaga ctaattttag ttagtggagg
     2221 aatggggggc tttcaatgtt gatgggtgaa ttccaacata atattgatgc aaaaggacgt
     2281 ttaatcgtgc cctcaaaact tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga
     2341 ttggatggct gtcttttcgg ctatccgatg agcgaatggg aaaatctgga agctaaactg
     2401 aacgaaatgc cgttggctaa aaaagacgca cgtacttttg tgcgattctt ctattcggct
     2461 gccactgaat gcgaacttga taaacaagga cgaatcaata tacccagcac gttaagaaat
     2521 tatgcagctt tgacaaaagg ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg
     2581 gatgagacac gatggcaaga tttttctgca gcagcagaag agaactttga cgaaattgct
     2641 gaaagtatga ttgatttcgg actataagaa taggaagagg ctgactaatg actgaaacat
     2701 ttcaacacta tacagtcatg ttgaaagaaa cggtagatgg attgaacatc aaaccggatg
     2761 gcgtttatgt agactgtaca ctcggcggtg ccggacatag tgaatattta ttaagccaac
     2821 taagttctaa agggcatctg tatgcgtttg accaagatca aaaagcaatc gatcacgcca
     2881 agctgcgttt agcttcttat gtcgaacaag gtatggtcac atttatcaaa gcgaacttca
     2941 gagaattaga agaacgcttg agtgagcatg tagataaggt agacggcatt ttgtatgatc
     3001 taggggtttc ctctccccag ctagatgaag cagaacgtgg atttagttat catcaagatg
     3061 caccgttgga tatgcgcatg gatcagtcag ctcctttatc cgcttatcac gtaattaatg
     3121 aatattccta ccatgaatta gtaaagattt tcttccgcta tggcgaggag aaattttcca
     3181 aacaaattgc tcgtgaaatc gaacgagtac gaaaaacaaa accaatccaa acaactggtg
     3241 agcttgtcga aatcattaaa caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc
     3301 ctgcaaaaag aatttttcaa gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag
     3361 aggcctcttt agaacaggca atccggttat tgaaagtcaa tggaagaatc agcgtaatca
     3421 cattccattc attggaagac agaattgtca aaagtatgtt taaagaatat agtacggtac
     3481 aagatctacc accgggactt cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca
     3541 ttactcgtaa accaatcctt ccttcagagg aagagctaac agaaaacaat cgatcacgca
     3601 gtgcaaagtt acgtattgca gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa
     3661 ttaaagaaaa tgcaagatta cgaatatgat ttacctgaat tggtggatca gccggaacaa
     3721 caaatgcctg attcttcttc gaatcaagaa atcattcttc cacaatcacc ggcaaaacga
     3781 ttaaagcaga tttctgcttt ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt
     3841 gctttatgtg ttttaacagt tatgctgcgc acaaatatca gcggagctga aaaagaaatc
     3901 acgtcaatac aaacagaaat caatgataaa aagcaagaaa aaacgagttt agaacaagaa
     3961 aaaaacgaac tttctcgtac tgaacggatt aaacaaatcg cagaagaaaa aggattgtct
     4021 attaatgacg acaatttaag gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg
     4081 tttatggaaa agaaaaatct aaatccgatg aacaatcgca aaaaagtagg tatcatttta
     4141 tttgctacga gtattggatt gttcttttta tttgccgtcc gtttctcgta tatcgttatt
     4201 ggaggtcatg ttgctggtac ctcattagct gaaaaaacaa aacagttgta ccaaggaagt
     4261 gaagtagtaa aagcaaaaag aggaacgatt tatgatcgta acggtgtggc gctcgcagaa
     4321 gatgcctcct cttattcaat taaagcgata ctctcaaaaa catatacctc tggggataaa
     4381 aaactgtatg ttgaagaaaa gaattttgat aaaatagcag aaatcctgca taagaatctt
     4441 tctatagaca aaaaagatgc actgaatatt ttagaggacg gcgcaaaaaa agagctgtat
     4501 caagttgaat ttggtagcta tgggaaaaat atctcccaag aaaccaagca aaacatcgaa
     4561 gcggacatga aaaaagaagg tgtcgctggt ctttattttg tcgaccatca ggctcggatg
     4621 tatccaaatg gcgtcttttc ttctcatttt attggatacg cagttccaga caaagatgag
     4681 aatggcttag ttggtaaact aggtttggag tctgcttaca atgatatctt gagcggtaaa
     4741 gatggtaaga ttatttatca aaaagataat tttcaaaatc ctctgccagg tacagtggca
     4801 gaagaagaaa aagctgtcga cggacaagat atctacacga ctttagacag cagactacaa
     4861 agttatttag aaacactgat ggatcaagta aatgaagaat atcagccaga agaattgaca
     4921 gcagtcctga tgaaagcaaa aaccggagaa atcttggcaa tgggacaacg acctactttc
     4981 aatcctgaaa caatggaagg attgactgga aaagatgcta tctggcgtaa tttcttggta
     5041 caagatagtt atgaaccagg atcaaccatg aaagttttca ctactgcagc tgctatcgaa
     5101 gaaggagaat tcaacgagaa tgaaaccttc caatctggaa aaatccaagt agaagatgct
     5161 acgatcaatg accatgactt tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca
     5221 tggtcaagta acgtcgggat ggtcatactg gaacagcgat taggcggacg atggtataac
     5281 tatttacaaa aactgggatt tggacaaagt actcattctg gattagatga tgaagtaaac
     5341 ggtgctttac ctacattaaa tattgtcgac cgagcaatga gtgcttatgg acaagcggta
     5401 ggtgtgacga atttccaaat gatgaaagct ttcacttcta ttgcgaataa cggtacaatg
     5461 attcaacctc gatatatcag caaagtggtg gatccccaaa ctggagaaga gcgaacaact
     5521 caaacagaag ttctagggca acctttttca aaagaaacaa cagaaaaagt ccgtgaatat
     5581 atgagagatg ttgtcgaaag cgaaaactac ggtattgctt atggtgtata tagtgtacct
     5641 ggatacaatg tatcggcaaa aaccggaaca gcccaaatcg cttctgatac agggggatat
     5701 caaacaggag atacagccta cctgtattcg attgtggaga tggttccttc tgaagatcca
     5761 gactatgtct tgtatctaac aatgaaacac cctaagacat atgatcgaat ggcattggct
     5821 aaaatcgcaa atccattgat gaaacgggca atggatttta aagaaactga agaagattca
     5881 gatacagaaa caaaaacaga aaaagtatcc gtagccgatt atcgtaatct tgaagcggac
     5941 gttgcagcag ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa
     6001 aaagtacaaa aacaatctac tgccaacggt gatcagttga tctctggaga aaaactgatc
     6061 ctatatactg gtggtgataa gctgatgcct gacgtaacag gatggtcaaa agcagatatc
     6121 atgaagctgg ggaaaatcct cggtattgaa gttagttttg atggagatgg ttattgtgtc
     6181 aaacaagaac ttgctccata tgaaaaaatc actgaagaca aacttagttt tactttagaa
     6241 gaataagaag agaaatagga gagaaaagag catggaatgg acacaagcat tgattcccat
     6301 tgtcagcagt tgtgcaatga caattgccgc aatgccgcta tttattggtt attttcaaat
     6361 gaaaaagcaa ggtcaggcga ttcgtgagga gggaccaaag tggcacaata gcaaagccgg
     6421 aactcctaca atgggcggct tagtgtttct gatcggctct attttgacag gaatttgggt
     6481 tggagcttgg caaaaacagc taactcctac tcttttcatc ttgctttttg tattagcgtt
     6541 atacggagta atcggctttc ttgatgattt tataaaaata ttcaagaaaa gaaacatggg
     6601 actgaactcg aagcagaaat tgattggaca gattctaggg ggaatcatct tttatcttgt
     6661 ttaccgttct gaaggttatc caggtgtatt gaatttcttc ggactagatc tacctttagg
     6721 aatcatttat ggtattttcg ctctcttctg gcttgttgga ttttcaaatg cagtaaatct
     6781 gacagatggc atcgatggat tagttgctgg cttaggaagt atctcttttg cagcttacgg
     6841 tctaattgct tggcatcagc atcagtatga tgtattaatc atttgcttaa gtgttttagg
     6901 aggtttactg ggtttcttcg tctacaacag aaaacctgcc aagattttta tgggagatgt
     6961 cggttcatta gcgttgggcg gacttttagc ggcaatttct ataatgctaa accaagaatg
     7021 gacattgctt ttgattggtt tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt
     7081 tacttccttt aagcttacag gaaaacgaat ttttaaaatg tctccaattc atcatcactt
     7141 tgaaatgtgc ggatggtcgg agtggaagat tgatacagtt ttttggttga tcagcattgt
     7201 gacttcgttg atcacattat ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat
     7261 caacatatga aaataagaaa gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg
     7321 ccaaattatt acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg
     7381 aaaatccgga agcacaagag ctgctttctt tagggataaa agtaataaca ggtagtcatc
     7441 caatcgaatt gttggatgaa gaattttcat tgatggtcaa aaatccaggg attccctaca
     7501 gtcatccttt agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg gaagttgagc
     7561 tagcttacga agtagcggaa tgcccaatta ttgggattac aggaacgaac ggaaaaacaa
     7621 cgacaacaac gatgacagga ctactactga atgcaggagc agatcaagga attgcacgtt
     7681 tagcgggtaa tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata
     7741 aaattgtgat ggaattgtct agcttccaac tcatgggaat cacagatttc cgccctcata
     7801 tagcggtgat taccaatatc tatgaagcac atattgatta tcatggtaca agaaaagaat
     7861 atgtcaaagc taaatggaac cttcaaaaaa acatgacaga aaaagattat ctgatcctta
     7921 actggaatca gtcagaacta caagaattag cgcaaagaac aaaagcacgc gtactgcctt
     7981 tctcgacaaa agaagtgttg gaagacggtg tgtatgctga cgattactca atatactata
     8041 aaaaagaaaa aataatggaa atatctgaac ttggcgtacc aggtaagcat aatgtagaaa
     8101 atgcactagc tgctatttct gtagcaaaat tgtatggtat atcaaatgaa gctatacgag
     8161 aaacgttgca ttttttccat ggcgtaccac atcgtacgca atatgtagga gaaatccaag
     8221 gcagaaaatt ttataatgat tcaaaagcaa ccaatatttt agcgactaaa atggctctaa
     8281 gtggatttga aacgtctaag gtcgtgttat tggctggcgg attagacaga ggaaatacat
     8341 ttgatgaatt aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa
     8401 aagaaaagtt gatggatgct ggaaaaaaag caggcatcga gacaatagta acagccgatt
     8461 ctgtagaaca agcagttcct ttagcattag aaaacagtac agatggtgat gtagttttat
     8521 tatcaccagc gaatgccagc tgggatcaat acccaaattt tgaaactaga ggaaacagat
     8581 tcatggaagc tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa
     8641 attttggtta caggcggagg aacaggggga catatttatc cagctcttgc ctttgtcaat
     8701 tatgtaaaga ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta
     8761 gaaaacaaaa tcgtccctga tacaggaatg cctttccata ccttggagat tcaaggattt
     8821 aaacgaaaaa tttccatgca taacatcaag acggtccgat tgtttcttaa gagtatccgt
     8881 caagcaaaaa aaatcctcag tgattttcaa ccagatatcg ttatcggtac tggaggatat
     8941 gtttccggag cggtagtgta cgctgcagcc aaaatgggta ttccaacagt tatccatgaa
     9001 caaaacagtg taccaggtgt taccaataaa tttttaagcc gatatgtgga tcggatcgct
     9061 ctttcattta aagatgcggc ttcctttttt cctgaaaaca aagcagttct tgtaggtaat
     9121 cctcgagcac aagaagtagc agatactaaa aaatcagata ttttgaaaac atttgatttg
     9181 gatccagaaa agaaaacagt cttgattttt ggcggtagtc aaggagcact taaaatcaat
     9241 caagcagtat cagttttttt aagtgaatat attccttctt cttaccaagt tttgtatgct
     9301 tcaggagata gatattatga agagatccta aataaaataa atcctgaaat acaaaatatt
     9361 agtgtccagc cttatatcaa aaatatggca gaggtcatgg cttccagtga tctgctggtg
     9421 gggcgtgctg gtgcaacttc gatcgcagaa ttcacagctc tggggttacc ggcagtgttg
     9481 gttcccagcc catatgttac gaatgatcat cagactaaaa atgcaatgag tcttgttcac
     9541 gcaggagcag caaaaatgat tgcagataat gaactaacag gtgaatcact aagtcagaca
     9601 gtcaacgaaa tcatgggtga tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa
     9661 caaggaatcc ctgatgcttc aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa
     9721 taaaggcggt ggtcttagtg agtaagaaaa aagaaccaaa aaaattgtct catccgtctt
     9781 ctgatgaaaa caatttgact ccttggcaaa aggctaaccg aaaatatctg gcagaacaag
     9841 agatgaaaaa ccaagagcca aaaaaagaag acataacatt agatacaccc ctttcagatt
     9901 ctgaagaaga aaaaacggtt caagaggaac agcaagaaga aagccagtcc gagaaaaaaa
     9961 tcgaattttt tgaagagatc aagcaaaaga aaaacggtgg accatataac ggttcttttt
    10021 tgaaccggct gcccaattta aaaagtcaac ggcacaaagt actatttcgt cggttagctt
    10081 tgatcatcac tgtattgggt ataccgctga tttttttgat ctattatgtt tctccttata
    10141 gcaaattaca agcagttgca gtatcaggca ataagacagt caacagccaa gaaatcatct
    10201 ctgatacaaa attgtcatta ggagaaaatg tttggggaca gtattttcat cgatctactt
    10261 atatcgatcg ccttaaaaaa gcacagccaa gaatcgaaac agcaaatata catttcaaag
    10321 ggatgaatga attcgatttg gatgtaacag aatataaaga gattgctttg atcgcaaaaa
    10381 acaatcaata ctacccagtg attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc
    10441 ctacaaaaaa tctccctatc ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa
    10501 caaagcaata caatcagtta tcatcagaat tacaaaaagc aatttctgaa atcaagtaca
    10561 cgccaagagc aagcaataaa aatctgatcc aattgaatat gaatgacggc aatcaagtta
    10621 ttgtaaatat caataatctt gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata
    10681 tggatgaaaa aggtgtgatc gacatggaag ttggtatctt ctcgcatcct tacggtagca
    10741 ctcaagaaac ggaagaatcg ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg
    10801 atcaaacaaa cgaatcaagt gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt
    10861 ctagcacaaa cggatcatct gattcaaatg attcaagtga aacaagtcaa atagaagata
    10921 atccaagtac aactccgtca agtttaaatg aaaattgata aaacaacttt aaaaagtaag
    10981 aatgtacttt ctaaaaacca gtaatttatg gtagaattgg aagcagtgaa tatatctatt
    11041 aataagtata ttttaaaacg gacgctagtt gttcgcttgt atatggaata gtaagtagga
    11101 gggaatccca ttcatggcaa aaacaggaat gtatgtaggc cttgatattg gaacgacatc
    11161 tgttaaagtt gtcgtggctg aatatatcga cagccaaatg aatattattg gagtaggaaa
    11221 cgcaaaatca gaagggatta accgaggcat tatcgttgat atcgataaaa cggtccaagc
    11281 gatacaacga gcagtacgac aagcagaaga aaaagcaggt atccagatca aaggcgtaag
    11341 tgtcggtttg ccagcaaaca tgcttgaagt agaaaattgc cagggaatga ttgcagttaa
    11401 cggagattca aaggaaatca ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt
    11461 tcgttcaata cctcctgaac gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga
    11521 tgggttcgag ggaatcaaag atcctcgtgg aatgatcggc gtaagattag aaatgtacgg
    11581 tctgttgttt acaggaccta aaacgatcat ccataacata cgtaaatgcg tggaaaatgc
    11641 cggattaatc gtcaacgaaa tggtgattac tcctttagcg ctgactgaat caattctttc
    11701 tgacggagaa aaagattttg gtacgatcgt cattgatatg ggtggcggcc aaacaacaac
    11761 ggctgttatg catgataagc aattgaagtt taccaatctt gaccaagaag gtggcgaatt
    11821 tgtcactaaa gatatctcta ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa
    11881 aataaactac ggggatgcat acccagaacg gacatcacca gatgaagaat ttccagtaga
    11941 tgtgattggt cagtcagaac cagtgaaagt cgatgaacgc tacttgtcag aaattatttc
    12001 agctcgaatg gaacagattt tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt
    12061 agagttgcca ggtggcgtcg tattgacagg cggagctgct agtcttcctg gtgttgtgga
    12121 cttggcacaa gaaatcttcg gcgtaaatgt aaaattgtat gttccaaatc acatggggct
    12181 gagaaacccg gtatttacaa atgtaataag tatcgtggat tattcagcaa acttgagtga
    12241 agtgtatcaa ttggcaaaaa ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt
    12301 agttgaacaa gaaacaacaa acacatatga gagctatgaa gcacctgaag aaacaatcta
    12361 cgatgagccg gaaccaaaag agtccggaga aaatgtaaaa aataaaatca agggtttctt
    12421 tacaaatatt tttgattaat tcatcctggg aggaataaga tatggaattc tcaattgata
    12481 ataatatcaa tgacggcgca gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg
    12541 ctgtaaaccg aatgattgaa gaaaacgtta aaggtgttga attcattaca gccaacacag
    12601 acgtgcaagc attaaaaaat tcaaaagctg aaacagttat ccaattaggc cccaaatata
    12661 ctcgtggttt aggtgctggt tctcagccag aagttggaca aaaagcggca gaagaaagtg
    12721 aacaatcctt acgcgaagct ttagacggtg cagatatgat tttcatcact gcaggaatgg
    12781 gtggaggaac cggtacaggt gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg
    12841 cattgactgt tggtgtcgtg acacgtccat tcacttttga aggaccgaaa cgtggccgtt
    12901 ttgctgcaga aggtatcgct cgtttgaaag aaaatgtgga tacattatta attatctcaa
    12961 ataaccgttt attagaagtt gtagataaaa agacaccgat gcttgaagct ttccgtgaag
    13021 cagataatgt tttacgacaa ggtgtacaag gtatctctga tttgatcaca gcgccaggtt
    13081 acgttaacct tgactttgct gacgtgaaaa cagtgatgga aaaccaaggt acagcattga
    13141 tggggatcgg tgtggctagt ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct
    13201 cctctccatt gcttgaaaca tctattgatg gagctgaaca agtacttctt aacatcacag
    13261 gcggtttgga tatgacattg ttcgaagcac aagatgcttc agatatcgta gcaaatgcag
    13321 caactggcga tgtaaatatc attctaggta catctatcaa tgaagaaatg ggcgatgaaa
    13381 tccgtgtgac ggtcatcgca acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc
    13441 gaccagctag acaagcacaa atgcaatcac ctgctcaaaa gactgtatta gatatggatc
    13501 aagctaaacc aacttcttca gaagaagaaa acagttttgg cgattgggat atcagacgtg
    13561 aacaaaatgt tcgtcctaga gttgatgatt ccaattttga caatatcgaa aaaaaagaat
    13621 ttgatacatt caaccgcgaa gaaacaaaat ctcatggcga tgatgaatta aatacgccac
    13681 cgtttttccg tcgtaaaaaa taaaggagag gaagagcaca tgattgctga caacttgcag
    13741 aaaatcaagc aagaaattca ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt
    13801 acattagtag ctgtaacaaa atctgttgaa tccgctcaag ctgaagaatt ggttagagct
    13861 ggcgttatga attgtgcaga aaaccgagta gataaattat tagaaaagaa acaagattta
    13921 tttgattttc ctgaaataaa gtggcattta attggtaatt tacagcgcag aaaggtaaaa
    13981 ttgattgtaa atgaaattga ttactttcat gcgttggaca gtttaagatt agcagaagaa
    14041 atccagaagc ggttagagaa agaattatcc tgttttattg aagtaaatgt ttctggtgag
    14101 gaatcaaaac atggaatttc accagatgag cttttaccgt ttgtggaatc tttagctgcc
    14161 tgcacaaaaa tcaaggttgt tgggttgatg actatggcgc ctaaagaagc ttctgatgag
    14221 caaatccata aagtgtttgg tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt
    14281 tatgcccatg caccatgtag tgaattaagt atggggatga gtcaagactt tcaaattgcg
    14341 attgaagaag gtgcaacatt tatccgtgta ggaacagcgc tattcaaaga cgaataaagg
    14401 aggaacaaaa atgccgcttt taaacagagg agcaattgct aattttttcg gattagctga
    14461 tgaggaagag tacgaatacc aagattatcc tgaaacaaca aaaaaggctg tacagccaac
    14521 taaaaaaaat acaacacaga atcaatcaac tcattctgta tctgctgcga atactcatga
    14581 aaaacaaaaa acggcgactt cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa
    14641 gaaaaaagta attgaactac atcaatcacc tacaaaaact gtagcgaaag aaagaacagc
    14701 ttcaagaacc tctacacaac ctaaagcaaa tcttgctgga aaaatcactg taatggaacc
    14761 aagagcttat tctgaagcta tggcgattgc taaacgaatc attgctggag aagcaacctt
    14821 agtgaatttt catttagtag aagaaaaaca agcgagaaga atcgttgatt ttctaacagg
    14881 aacagtttat gcactagatg gagatatcaa acgtgttgga gatgagattt ttctttgtac
    14941 accgactggg atggagattg acagttcaac cgctcaatct tttgcagatg cgaatttgtt
    15001 tgatctgtaa gaggaggaac tacgatttat actattgcac tattacttca tttacttcaa
    15061 cgggcagcct atttctatag cattatcttg atggtatatg cattgctctc ttggtttcca
    15121 ggcgggtatc aatcagggct aggacgatta ttagctaaga tctgcgaacc gtatatcagt
    15181 ttgttcgatc gtctgccttt gaggattgga cccttggatt tcacaatttt aggggcaatc
    15241 ctgatcttga acttagccgt acaaggttta gcacagatcg ttatatggat aactgtccat
    15301 ttagtttatt gattgaagca acaaaatctt tattctcaga atagatagga ggggaaaatt
    15361 ttggatgcaa atgtatacca gcactttcgg gcagatgaac gtacatttat cgattcggta
    15421 ggcgattgga ttgaacaggt tcaaggacaa tatgcaccct atctgaccga atttcttgac
    15481 ccaagacaat cctatatttt agaaacactg attcgacaaa gcagtgattt gcgtttcatg
    15541 ttttatggcg gatatgaaca ggcagaaaga aaaagatgtt tgattttccc cgattattac
    15601 gaacctgtag aggaagattt tgaaacagca ttgtatgaaa tccattatcc tagcaaattt
    15661 gccagcctaa gtcatgggaa aatccttgga actttagtgg gaacggggat caaacgctct
    15721 tactttggcg atatcatctc ggacggtgat cgctggcaag tatttattgc aaatgaggtg
    15781 gatcattttg ttgctgcaca agtaacaaag attggaaagg ttgctgtacg cttggaacca
    15841 atcaaataca cagaaatgat tatgccgaaa gatagctgga cacaagaaag aggaacagtt
    15901 agttccatgc gactagacag tgtgatctct gagatttata atatttccag acagcggtcc
    15961 aagcagctga tcgaagctgg taaagtaaaa gtgaactgga cagaaaatac aagacctgat
    16021 tttgtacttg aactattaga tattatttca gtcagaggtt atggcagagt ccagatccaa
    16081 gaacttgagg ggaagacgaa aaaagaaaag tacagggtac tgttcggtgt attacgtaaa
    16141 taaaataaaa aggtggttta ctcatggctt taactccatt agatatccaa aataagacat
    16201 tcccgacaaa aatgcgggga tataatcaag acgaagtaga tgatttctta gatttagttg
    16261 ttcgtgacta tgaggaattg actcaaagaa atcgagaatt agaaaaagca gtcaaacatt
    16321 ctgaagaaaa acttgagtac ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg
    16381 tagctcaaga tacggctgat aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg
    16441 ttacttctgc acaaaacaaa gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc
    16501 aattaacaac tgatgcagaa gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag
    16561 caagacagct tgcgacagaa actgaagatc ttaagaaaaa aacacgagta ttccatcaac
    16621 gtatcagctt gatgcttgag tctcaattag aacaagtgaa aagtccggaa tgggatgaaa
    16681 ttttacaacc attctccagc tacgtgactg acagccatga agtgatcaaa gaagttttag
    16741 caaaacagct tgacaatgaa aatgatactg atgtaaactc agacacagga gtcatagaag
    16801 cacctatgac ggcatttgat acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg
    16861 aaaacgagta gaacagaaaa gcactttgtt attcttaaag cgagccgatg agagtggaag
    16921 ttcggtaaga cactgcatgg tgaaagatcc tctacttgag acctgctttt ccgaaattag
    16981 agtaagaagc agcggctgac ctcgttattg gtcacagaga gtttgcacta gatagatgca
    17041 actaaatttg ggtggtacca cggcatgttc gtcccttgcg gctgaacatg cttttttgtg
    17101 tccggaaaac aaataaagga gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag
    17161 gaaaaacaaa attccctatg cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag
    17221 aatgggaaga aaatcgaatc taccaaaaaa gacaagagtt gaacgaagga aaacctagct
    17281 ttgttcttca tgatggacct ccatatgcca atggtaatat ccatttagga cattcattga
    17341 ataaaatcag taaagacatt atcattcgtt caaaatcaat gtctggctat cgtgcaccat
    17401 atgttccagg atgggataca catggattgc cgattgaaca agtattggct aataaaggcg
    17461 tgaaacgtaa agaaatgtcc atggcagaat acttggaaaa atgtcgtgaa tatgcacttt
    17521 cccaagtaga taagcaacga gcagatttta aacgcctagg agttgcagga gattgggaac
    17581 atccttatgt tacattagat ccttcttatg aagctgcaca agtacgcgta tttggtaaaa
    17641 tggctgaaaa aagctatatt tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg
    17701 agtcttcttt agcggaagct gaaattgaat ataaagatgt aaaatctcca tctatttttg
    17761 tagctttcaa tgtcaaagac ggcaaagata ttcttgatga agatacagct tttgtcattt
    17821 ggacgacaac accatggaca ttgccagcaa accaaggtat tgctgtaaat ccaaaattta
    17881 cgtatgtatt ggtggaagcc gatggccgta agtttgtcgt agcgaaagat ttgctagaaa
    17941 cagtccaatc agaaatcggt tgggaaaatg taacagttct aaaagaattt gctggacaag
    18001 aaatggaata tatgactgca agtcatccat tctatgaacg tgaatcactt gtgattttag
    18061 gcgatcatgt tactttagac gctggtactg gattagtcca tactgctcca ggacatgggg
    18121 aagatgacta tatcgctggt agcaaatata aactatctgt agtgtcacct gtagactcaa
    18181 aaggattgtt tacagaagaa gcacctggat ttgaaggtat cttttatgac aaagcaaatc
    18241 ctatgatcac tgatctatta aaagaaaaag gagctttgct gaagctagat ttctttactc
    18301 atagttatcc tcacgattgg cggacgaaaa agcctgtgat ctatcgggca actccacaat
    18361 ggtttgcatc tatcgataaa ttccgtcaga atattttgga tgaagtagaa aaagtagagt
    18421 gggttatccc atggggtaaa acacgtttgt ataatatgat ccgtgaccgc ggcgattggg
    18481 taatttcccg tcagcgtgca tggggagtac cacttccgat tttctatgct gaaaatggag
    18541 aagcaatcat cacaccagaa acaatcgaac atgtggcaaa attatttgct gaacatggat
    18601 caattatctg gtttgaaaaa gaagcgaaag aacttcttcc tgaaggattt acccacccag
    18661 gttctcctaa tggcgaattt acaaaagaaa aagatatcat ggatgtctgg tttgattctg
    18721 gttcttctca tgaagctgtt ttacgtcagc gaccagaatt atcattccca gcagatatgt
    18781 atttagaagg atcagaccaa tatcgtggct ggttcaactc aagtatcacg actagtgtgg
    18841 ctatcaatgg tgtagcacca tacaaatcag ttatttctca aggatttgct ttagatggtg
    18901 aaggccgtaa gatgagtaaa tctttaggaa acatcattgc accagaaaaa gtgatcaaac
    18961 aatttggtgc agatatcctt cgcttatggg tatctagtgt agattatgaa gcagatgttc
    19021 gtgtttctat ggatatctta agtcaagtgg cagaagtata tcgtaaaatc agaaatacga
    19081 tgcgtttctt attagcgaat acagaagatt tcgatcctaa aaaagatact gtttcgtaca
    19141 atgatttacg ttctgtagac aaatatatga ccgttcgatt gaaccaagta atcaaagaaa
    19201 tccgagaaga agggtacgac aaatataact tcatgcatat ttaccgtaca gttatgaact
    19261 tcttgacggt tgatctatcg tcattctacc tggattttgc taaagatgta gtttatattg
    19321 aagcagaaga tgattatcaa cgtcgttgca tgcaaactgt tttctatcaa acagctgtag
    19381 ctttgacaaa attattgact cctatcattc cgcatacagc agaagaaatc tggtcattcc
    19441 tgaaagaaga agaggagtac gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa
    19501 accaagaaga attgatggat acctggactg ctttcatgga cttccgtgat aatgtcttaa
    19561 aagcattgga agaagcaaga aacagcaaat tgattggtaa atctttggaa gcaaaactaa
    19621 ctgtttatcc aaaacaacaa gttcgtgaaa tgttaaaagc tttggatgca gatattgcac
    19681 aattattgat cgtttcacca gactactttg aagttatggc agcagatgaa caagtgccag
    19741 ataacgctat ggtatttgat gatgttgcta ttacagtgga aaaagcagac ggggaaactt
    19801 gcgatcgctg tcgccaagtt cgtaaagatg taggagtaga tgaaaaactt cctcatctat
    19861 gcggtcgttg tgcaaaaatc gttgaagact tctatcctga agctgtagct gaaggattcg
    19921 aagaaaagta aaagggaaaa gggggtgtga catgttcttt gtcacacccc cttttgttat
    19981 caatcgttta attttccacg ttcacgatac cagttatctg gttgccagat ccattcgaat
    20041 ccgtcttttt caagtagttc gaaagctgct ttaggtccca ttgatccagc tttgtagttg
    20101 ggaaaatccg gttgtgtttg gtcccaagct tttcgaatga catccacgat atgccaagat
    20161 tgagcgactt cttcccaatg agagaagttc gttccatcac cattcaacgc atctaatagt
    20221 aatttttcgt aagcttctgg gctattttca accatttctg cactatttct aaattccaat
    20281 ttgattggtt ccgtttcaaa tccttgccct acttctttcc cattcaagga taaagaaaat
    20341 ccttcagttg gctgaatata gatcgttaga acattaggtg gaagtgtcgt atcgtcacat
    20401 ggttcatcga cagatgtttt gaatacatta acaggaactt gtttgaatac gatgttgatc
    20461 cgagtccctt tttcagtcaa acgtttgcct gtacgtacat agaaaggaac acctgaccaa
    20521 cggaagttat cgatagtgaa tttgcctgcg acgaatgttt cagtctctga ttgagcatcc
    20581 actttgtctt cttcgcgata gcccttgaat tgtacaccat ccaattcacc ttgagcatat
    20641 tgcccgcgaa cgaagttttc taacacttct tcttcgttat aaatgcggat agctttcaat
    20701 gctttgatct tttctgtacg gatttctttt tcggaaaatg ctactggcgg ttccattgca
    20761 agtaatgcta caacctgtaa aatatgattt tgcaccatgt ctttaagtgc gccactatga
    20821 tcgtagtagc ccccgcgatc ttctacaccg aggttttcgc caaaagtaat ttggatattg
    20881 tcgatatagc gattgttcca ttgagcctca aaaatattat tggcaaaacg gatagctgag
    20941 atgttttgga tcatttcttt tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg
    21001 aatacttctc tgatttgctg gttcaattca aaagcagact ggtaatcaga accgaacggt
    21061 ttttctatga tcaaacgatt gaagccattt tttgtcataa tattttgtga tttcaaatga
    21121 gaaacgatcg ttccaaaaaa ttgaggtgcc atagccaaat aatagacacg gttaccgttt
    21181 aattgatagt gtccatctaa tcggtcagac aattctttta atgtctgata atgcgttgaa
    21241 tcatttacat catgggattg atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct
    21301 gaaggattca atgcacggat tgtttctttg acaacttcac ggtagtgctc atcgctccag
    21361 ggacggcgag cggttccgat tacagcaaaa tgctcgccta aatttccttt tctgtaaagg
    21421 cggaacaaag acgggtacaa tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta
    21481 aacaatacat ttttttcatt agacatgtgg atacactcct ttatatttta tcgacaaaaa
    21541 tcgtgctggc cgccttaaag ctgacgggga ttttctttct atcattttgg atagtgatcg
    21601 gtccttcata agctgcgatg tcaacgatct catattcttc gttgatattc aggtcaatag
    21661 tgactaagta atccaacaat tctttttcat caagcactcg tgcgatacgc acttttgtac
    21721 cgacaggata ggactccaat gtttgtcggc gttcttcatg gacaacttcc tcttttttag
    21781 gaatcgatcc tccatgagga caatgttcag gatgattcaa atagttttct aaatgatctg
    21841 caagatgttc cgaagtcaca tgttccaata cttcagcatc atcatggact tcattccagc
    21901 tatagtctaa atgttcaact aaaaaaactt cccataagcg atgtttgcga atcagactgc
    21961 tagctttttg cagtccgctt gcagtcaatt gtacaccttg ataaggggaa tgttctacaa
    22021 gctcttcttt cacaagttta gaaatcattt cactgacaga tgcagctgag acatctagac
    22081 tagatacgat ttgtttattg ttgactttcg ttgtatctcc acctagttct aaaatgattt
    22141 tcaaataatc ttctcgattt ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc
    22201 attagtaagt gtaacaaaaa aaacgttaat tgactatttt tctaaataaa aaacaaagga
    22261 tttttagacg tttttacaac aaaaaacgaa agatgataga gaagactcat tctttcgttt
    22321 ataaaggata tatatataca ttgggaggga tctatttttc agataacaac ttatatgaag
    22381 tgtctgcaga ttcgtcataa agagtttgtt cgggataaga aaagataaga taaaaaccat
    22441 actgatcacg tacgactaaa cgatattcat ctatttctac cacagatgtg ccaagagact
    22501 gaccgttgat caaaaacgtg taatcagatg taatctgtaa ttgttgtttt tgctggctat
    22561 cactaaagaa ccatgtacct tgatactttg agagtccttc tgacttttcc gcttgttttt
    22621 tctttttcaa gtaaataaaa cttgagagaa cggtaatgag tgaaccaaga aaatagatca
    22681 ttttttttat caccaaatca ctcctttatg agaaaataat cgcatagaca tattctttct
    22741 tctttatttc attgtaccga ataaaagcta aaatttctct aagactttcg actggttttt
    22801 ttactgtttt tctacaaatt atgataggat acatagtagg aagaaaggtg acataatttg
    22861 aagtatcagc aagtggaaat agcttttttt attgaccgcc cgaatcggtt tatagcacat
    22921 tgtatgaaaa aagacggaga aataattatt gcgcatgtca aaaatacagg tagaggaaaa
    22981 gaggtcctga taccaggcgc tgaagtggct attgtatttg ctcctggacc taaacggaaa
    23041 accgcctatg atctgattgc tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa
    23101 ttgcctaatc ggctggcaat tgatggtatt ttagacggca cgatcctttt accaggatta
    23161 actggtccat ttacggtata caagcgtgaa gtaacttatc ttcactccaa atttgatatt
    23221 tatttagaga cggtggtcgg acaaaaagca tttgttgaag tcaaaggtat gacacttgaa
    23281 aataaagcga ttggtgcttt tcctgatgcg cccacactca gaggattgaa acatatagga
    23341 gaattaacgt atgccgcaca agatggttat gccgcttatg tcttgtttat tgctcagttc
    23401 gaacatcttc atctggcaac gatccatgaa gaaatgcaac cagcactagc tgatatggtt
    23461 cgacatgcgc agcaaagcgg agttcaaatc ctcgcctata attgtcaagt atcggcagat
    23521 gaggtaactg tcaagcaggc cattccattt gatgtagata tcccttttga agatcctata
    23581 caatagaaag aaggtaaaat aatgatcaac ttaggtatta tcggaacaaa ttggattact
    23641 catcaatttg tccaagcagc tttaagcaca aaaaaatatg aattgacagc ggtatattct
    23701 agacatttgg aaaaagcaca ggaatttggt tctcattatg aaggggatat cgaattcgca
    23761 acagatttga agactttttt caatattact catatggaca ccatttatat tgcttctccg
    23821 aatagtcttc actttagtca agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt
    23881 gaaaagccag ctttttcgac gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa
    23941 cacaatgtgt atttttttga agcagcaaga aatatccatg aatcaggttt taaaaagatt
    24001 gccgattact taccattaaa agacgagata atcggtgcaa atttcagcta tatgaaatat
    24061 tcttcgcgct atgaccaagt attagatgga gaagaaccga atatcttctc gactcatttc
    24121 tcaggtggtg cattgatgga tttaggtgtt taccttgtat atgcagctgt tgcttggttt
    24181 ggaatgccaa aagaagtcca ttatttccca agaaagattt ctactggtgt agatggtctt
    24241 ggatggggga tcttacgtta tgagaatttt gatgtatcga tccagccagg taaaatcggt
    24301 gattcttatt taccgtcaga gatttatttt gactcaggaa cgctagtgat gaatggagtc
    24361 aatgcaattg aacaagcaca atttcatgat cgcgcgcata aagaaatcat tgatttggcg
    24421 gtaacagctg cagaaaatcc aatggcagat gaagcagagg attttgccaa tgtcatggct
    24481 catccgaaag atccagcttg gggattgttg tatgaagagt ggatcgagct agctagaaat
    24541 gtgaaccaag tgatttatga tttaagaaaa aatggcggca tcaaattcga tgcagataat
    24601 gaggaagatt tttaatgaac gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat
    24661 atgaggataa agggtttatc tcaccttctc ttattcaacg agcagttttt cagccactaa
    24721 gtaataaaca aagtatagta gcggtttctc ctacaggttc aggtaaaaca ctggcttatc
    24781 tttggcctct actactaaac gtggaaccag gtgaagcaag tgcgttggtc atttttgctt
    24841 cttctcaaga actagctatt caagtagcag atgttgcacg tgaatgggga aaagacaaag
    24901 agttgaaagt ccaatctttg gtgggcggag ccaacgtcaa acgtcagatt gagggtttga
    24961 aggaaaaacc agaaatcttg attggtacac caggaagaat attggaattg atgaaagcaa
    25021 aaaaaataaa agcacatcaa gtgaaaacaa tggtatttga tgaagcagac cagctatttg
    25081 acgctgggaa cagccaaatc attgatcaaa ttcttcatca agctccaaca gagtatcagc
    25141 tagctttttt ttcagcgaca gcagatcgtt cccttgaaag cattgaaaaa atcactggga
    25201 aaacgtttga gacaattgac gtgacagcag aggacgattc tcgaaaagga ctgagacatt
    25261 actttttacg tgtaccgata cgaaagaaag atgactactt gagacgtctc acgcatatag
    25321 aagattttca tggtctgatt ttctttaatc aactgactga attaggcgta atggaagaaa
    25381 aattgctgta tagaggtgta ccggtaggaa gtctggcctc tgatcagaac aagctactcc
    25441 gaaaagcagc gctagaacaa ttcaaaaaga aaatggtcag agcacttttg accacagatg
    25501 tggcggctag aggtttagat attacaggtc ttccttatgt agtgaatgca gaagttccgt
    25561 taagtgaaga agcttatttg catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa
    25621 tagtaattac actcgtacaa gataataatc taagagattt aaaacggctt ttacgtccat
    25681 caaatataac acttgaagag atttttttat acggaggtaa gctgcaaact gagcagccaa
    25741 ccaaagaaga tgcagctcct gcagtaaata aaaagtatgc gaaaactttt tctggaaagg
    25801 agcagaaccc agaatataac aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa
    25861 aagagagaaa taaaggagca aggcgcaaat aagcgccttt ttatgctatg attatgaaaa
    25921 cgggaacaaa attggttgcc agaggagggg ttatgtgaaa gaacgtcaag aagagatctt
    25981 gcagctattg acagaaaacc aacaaggatt gacagcaagt gatgtagctg agcgcttaac
    26041 tatcgatcga agcaatgcaa gccgatattt aagtgaacta tacaaggccc atcatattgt
    26101 aaaaaccgct ggccgaccag tcgtttactc tttgccaacg gaaaagtcaa aatctgatga
    26161 agtgcatgtc gactcttcta cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc
    26221 tttaaaagtc agcatacagc aggcaaaagc tgcgattctt tatccaccgc gggggttgca
    26281 tacgattatt tttggagaaa ctggtacagg gaagtcgatg tttgctgaat gtatgtatca
    26341 ttttgcaatc gattcagaga tgctttctgc agatgcgcca tttgtttctt tcaattgtgc
    26401 ggattatgct caaaatccgc aactgctctt tggacacatc tttgggatca aaaaaggggc
    26461 ttacacagga gcagctcaag atagtcccgg tctaatagcc aaagcagacg gcggtatatt
    26521 gtttttagat gagatccacc gtttaccgcc ggaagggcag gaaatgctgt ttactttcat
    26581 tgataaaggt atttaccgtc cgcttggaga aagtgcacaa gtacatgaag catctgttca
    26641 gatcattggg gcgacgactg aatcttcaga aagtttctta tctacgttca atcgacggat
    26701 tcctatggca atcaccttgc cgaatcttgc ttcacgttca ctggatgagc gttatgaaat
    26761 catctctctt tttatcaaac aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa
    26821 agaagcgatt cttgccttca tgctttatga tgcggaagga aatatcggtc aagtgaaacg
    26881 agacttgaaa ctagtctgtg caaaatcttt tttacattac cgtactcatc atgagaaaaa
    26941 gctaatcatt cgaaaagaag aactttccct tcaagtacaa aaaggattac tgaaaatcaa
    27001 ggaaataccg gaacgtctcg accgattcat ggacagcaaa agtcagtatt tgacatttga
    27061 accaggattt gctgatgttg tctggtcaca agatccagaa cgaaacatgc aggtctataa
    27121 cgatattgag gaaaaagtca cttctctttc cgaaaccggt gtggagaaca ttgatctaga
    27181 gaccctcatt tcaaaagatg tggatgctta tttccagaca tatgtcaaag aattgactaa
    27241 aagttcgatt cccaaagatc ttttgccaga tgaaatctgg cggttaacca atcaattgta
    27301 tgatgtggct gaaaaacggt tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc
    27361 tttgcatctg caaagtacat tggatcgtgt gagagaaggt catatgatcg tccacccaga
    27421 tttgaataac gtacggaaaa attttaagag ggaattccag gtagcaatcg atctttcaac
    27481 gatcattgaa gaagaagaac aagttgagat tccttttgat gaaatcggct ttattagtat
    27541 gtttctttct atcaatctag gagaaagtga gcctgttaaa gaaaacaaag tagatgttct
    27601 catattgatg cacggcagac agactgcttc aagcatgttg gagatggcac aagaactatt
    27661 aggaacaaaa gtagggaaag cttttaatat gccactggtt atggaagttc aaacaatgta
    27721 tgaagaagtg ttacaatatg tcaaaaacta tcaagagcat ttgacgaatg gcctactctt
    27781 gttaacagat atgggatcat taaacacctt cagtgatttg atttatgaag agactggcat
    27841 tcggacgaaa gctatcacga tgacaagtac catgattgtg atagaagcga tacggatggc
    27901 aagtgttgga agaagcttag aagatattta tcaaaatatc cagctttcct ttgaatcgat
    27961 cgtacgtgat cagttcaaag cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat
    28021 cgtgacatgc tttactggtg aaggagtagc tgccaaatta tatcagcgta ttatgccagt
    28081 gattaatcaa aataaggtag aactgatcca gatgcagttt ttagaacgtg aatcattcaa
    28141 aaaacacatc gatgccttat tggaagaata tgagatcaaa gcaattgcgg gaacagttga
    28201 tgttgattat caaaatatcc catttttttc agcctatgac gtttttgatg atgaaaaact
    28261 aaatatttta aagcgtatag ctgatgatga gatcccgaca gctaaaattg tcgattcact
    28321 tgctggatca ttaagcgatg tcttttctat tgatgaactg atccgttcat tgcaaaagac
    28381 agttcagcag atccagaccg atttgtcgat tatcgtggaa cctggagtag atactggaat
    28441 cgttatccat ttagccttca tgttagacgg attgatcaaa caagaaagcc atcggcaatt
    28501 tgagaaactg tctattttct caaaaattta tcgtttggaa atggatatcg ttcgggcaaa
    28561 cttgatggca ctagaacgaa aatatcaggt aaaaattcca gaagatgata tcgcatttct
    28621 tacacaaatg tttcttgaaa ataaaataaa aaaatgaaca cactaatcat actgtttaca
    28681 cactaaacat ctgtgtgcgc actataaaaa agagctaaaa agcatgaaaa cgctatttca
    28741 gctctttttt ttattggcat gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag
    28801 tgttaaaaat ggcaaaaaaa acaatcatgt tagtttgttc agcagggatg agtacgagtt
    28861 tattggtcac aaaaatgcaa aaagcagcag aagaaaaagg gatggaagcc gatatttttg
    28921 cggtctctgc ctcagatgca gataataatt tagaatctaa gaatgtcgat gtattattat
    28981 taggtcctca agtacgtttc atgaaagctc aatttgaaca aaaacttgcg cctaaaggta
    29041 ttccattaga tgtcatcaat atgcaagact atggcatgat gaacggagaa aaagttcttg
    29101 cacaagcaga aaatctaatg aaataaatca agtatgcagt tgggggtata acaacgtgga
    29161 agatcaacaa aatctagaag ccatcatggg gttaatcatg tttggcggta atgcaaagag
    29221 cgatgcaatg gaagcgattg cggctgctaa aatcggtgat tttgagttag cggataggaa
    29281 aatcgctgat gcagaagaat cacttataca agcgcatcat gcacaaacag acatgttgac
    29341 acaagaagca caaggaaatc atatgcaagt gacattattg actgttcaca gtcaagatca
    29401 cttaatgaca tctattgcgt ttactgattt agcaaaagaa atcatcgaac tttatcgtcg
    29461 cgtatacagc gaataaatac aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg
    29521 attttttatt ttgttataag gagaactctc tttatataaa aaacttatag gaggaagtaa
    29581 aaaatgaacg gactatcaag ttggatggag aaatacatcc tgccggttgc cgcaaaaatc
    29641 ggagcgcaaa aacatttagt tgctttgcgt gatgctttta tcggcacact gcccgcaaca
    29701 atggcgggat cagtcgctgt aatgatcaat gcaattattc gtgatttacc gcaacagttt
    29761 aacagtacct atgctgacag tttagcagca gacaaaggaa tctttgccgt attgaatgtc
    29821 atcatcggaa tcaatggttt tgtctggaac ggtacactag caatcgccgg attgatcttt
    29881 gccttttctt gggggtataa tcttgcccga gcttatggag taaatgatct agctgggggg
    29941 atcgttggtt tagctacctt gattcaaggt atttcctttg gtccagcatt atcagcagca
    30001 ttagatgtga atgttccttc aaatgtcgca gatgcaatca atagttctga gattggtgca
    30061 acgattgcag atggcacact gtcagttgga ttatggggtt ggctaaaact agatcatttg
    30121 aatggaaacg cttattttac agttatgatc atgggagcta tttcagtcat tattttttgt
    30181 aaattgatgc ttgccaacat tacgatcaaa atgcctgatt cagtaccacc agcagtatct
    30241 aaagcatttg cagccatttt accagcgacg atcgctttat acgtcattgc tattatcaat
    30301 tatatcgtag gtcgtgtagc tgatgggcaa ctgatcattg atttggtgca aaaatatatt
    30361 gcagaaccat tcctaggatt atctcaaggg ttgggagcag tattgatcgt cacagtattt
    30421 gttcaagtat tctggttctt tggtattcat ggaccaaatg tattggcacc agtgctagag
    30481 ggaatctggg gacaagcaca actaatcaat attgatattt tccaaaaagg ttataacgga
    30541 aaaactggta caccagccgt gttagatgca atcgaagatg gaaaagctta tatgtgggtc
    30601 cgcggatcgt tcgatgcttt tgcatggttt ggcggatcag gcggaacgat cgtcttactg
    30661 atagctatct tgattttctc taaacgtgca gattatctaa cagtagctaa attatccatt
    30721 gggcctggta tcttcaatat caacgaacca gtgatgtttg gtctgccaat cgtattgaat
    30781 gcaatcatgt ttatcccatt cttgattgct ccagtagtag caacagcaat tggatggaca
    30841 gctacttatc ttggcctagt cgctccggta tcgcagcaag tcacatgggt agtaccgccg
    30901 ttcttattgt cattcttagc aacaggagcg gattggcggg caccgatcgt aacattagta
    30961 tgtatggtcg tgactttctt gatttgggca ccatttgtca tctctgcaaa caaaatggac
    31021 cctgccgcag gcgaacaaca ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt
    31081 cctcataatg aattttcgag gaggaactga aatgattaca attgccagtg caatgcaaca
    31141 agatgaaatc aaagaattgt tagaaggata caatgaagaa ggaatgactt ttacatttaa
    31201 agaaaaacaa ggaatcaagc tgttctttga aacaagtgca gaagatccgg aagttgcggc
    31261 taaaaaagca aaggaactaa tcaaagcaca gccttgggga acagtccttt attttcaatc
    31321 aacagctgtt tagaaaatgg tttaaaaaga taggatggtt gagatgataa aaaacggttt
    31381 atttttatgt agtattggtt ctttgatcgt gttgtatagt ttgaatccac attcgcaaag
    31441 gtatgatttg tatagtatgg tcacaggtct ttttctaaca ggattaggaa tctatttctt
    31501 catgaagggg aaaaaaagag aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa
    31561 aaattaaata ttccagcatc gtttttttac gataaagtta tggactctgt ccttttcgat
    31621 gtacgtaaag cgaccggcaa atcagtaact agaaaacagc tgaaaaattt ggagtacgtc
    31681 aaacaattct cgcaaaataa ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag
    31741 tcttataagt ttcgtacatc gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa
    31801 ccattggacg attcgacctg tgaggttcat tacacagaaa aaatggagtc ttatggcatg
    31861 cttcaaaagt tgaatgatat gcttttagga acaatcttga tgtattttaa acggagacgt
    31921 ttcaaaaaaa tgctagatat gatcgaagca acgtattaaa tcaatgaaac atggtactat
    31981 ttagttattg aaaaatgaag taagctagaa ggaatttttc ataggaagat agatctttcc
    32041 tgcgaatcct cactagcttt tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag
    32101 agctaggtgt atcttcgatt gaggaaatgt ttgatttagc cacttatgca tttaatgggg
    32161 cagatacacc tgaaaggcga gaacgttttc gtacacttgc ggagaattct tggaattacg
    32221 gattttttga tgaggaggat cggctaacaa gtcaagtcat ggcgacccct tttccagtga
    32281 atttttacgg acaagagtac ctaatggccg gcattggcta tgttgcttct tatccagaag
    32341 caaggggaca aggcgggatc aatcagatca tggaaaagat ccttgaagat tgccgtgatc
    32401 gtaaagtaag cttatcttat ctggcgccat tctcctatcc cttctatcgg cgctatggct
    32461 atgaacaatc atttgataaa atcagttatc aactaaacag tcgtgatgtg ccgtttatca
    32521 aaaaaaaatc tggaaaaata aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa
    32581 gaatttatga acagatgccg gaaaaccaac gagctggtct gaagcgcgaa gactggtggt
    32641 atacgtataa attcaagcaa aaaggcaatt accagtttgc tttgcatttt gatgataatg
    32701 aggaagcaac gggatatatc gtttatcagc ttgcaacgtc ttcctttatt attgcggagt
    32761 ggggattttt ggatcatgat gctttttgca gccttgttcg cttcgtaagt tctcacaatg
    32821 gtgcttttga aacatttgaa tattcatgcg gctacggcgg taatgatcaa aattatctat
    32881 tagaaaatcc atttgcttct gtaatgatca caccttatat gatggcgagg atcgtagata
    32941 tttctttgtt tttggaaaat tatccattta aaaagagaca agctgcattt gccctacaaa
    33001 tagaagaaga tcattatgcg aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg
    33061 gaaaaaatca tctctcaaaa gtagaacaaa caaaactccc aatcgttcga ggaagtatcc
    33121 aacatatcac gcagctatta ttagggtatc gtaaagtgga agagttggta ttccaagaaa
    33181 gaatacaggt gacaaaagaa tatgaagaaa cgttaagctc aatcttgcca cagcaacagc
    33241 caattcttaa tgactatttt tgataaaaaa cgggtgatga caatattttt gtcaccaccc
    33301 ttttgttttt catttggcat ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa
    33361 tttgaatttt ttgttttctt ttgttttagg tgttttaggg ttttcttttt tgtggtcttg
    33421 gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc gttctgcgta
    33481 tactttttct tttggcttat cattcattgg aaaggacctc cctacattat ttcattctac
    33541 ctctagttta tcatctgtta aggactagtg gaaacgtttt gcctaactta taagctgatt
    33601 tttttagcag ataagcttgc aagtaaaaaa caaacaagct acaattgatt tattgaaatt
    33661 gttcctgtag ctcagtagga tagagcaatc gcctcctaag cgataggtcg ctggttcaat
    33721 tccagtcagg aacgtataaa taacccctgt atcctttgcg gtacaggggt ttgttttgta
    33781 tttgtgccaa aaatgtgcca aactagttat catttttata taaactatcg aacacagtat
    33841 tgattttttc tttatcctga tcttcaagct ctcgaacaat atgaagataa gtagacattg
    33901 tagtttctaa actgctatgt cctaaacgtt tcg
//
LOCUS       DAXWLG010000011        32681 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXWLG010000011 DAXWLG010000000
VERSION     DAXWLG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.011"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(317..388)
                     /locus_tag="ACGE4T_000394"
     tRNA            complement(317..388)
                     /locus_tag="ACGE4T_000394"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Ile,seq:cat)
     gene            complement(567..1586)
                     /locus_tag="ACGE4T_000395"
     CDS             complement(567..1586)
                     /locus_tag="ACGE4T_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8385709.1"
                     /translation="MKKKITVTAMSLLMALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAIKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVNANGVS
                     RPDVNQSYSTYGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(1597..1794)
                     /locus_tag="ACGE4T_000396"
     CDS             complement(1597..1794)
                     /locus_tag="ACGE4T_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8385710.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(1817..2110)
                     /locus_tag="ACGE4T_000397"
     CDS             complement(1817..2110)
                     /locus_tag="ACGE4T_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385711.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPITASFSKITETLSKVSR
                     NLDLLTKDLEASKSDRITIHEELKKHDGRLDTHAEKLVEHTEQIKTLFRERSR"
     gene            complement(2147..2284)
                     /locus_tag="ACGE4T_000398"
     CDS             complement(2147..2284)
                     /locus_tag="ACGE4T_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFF8385712.1"
                     /translation="MYSYDDIKMMYDWNCFTADQVRQFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(2286..2693)
                     /locus_tag="ACGE4T_000399"
     CDS             complement(2286..2693)
                     /locus_tag="ACGE4T_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385713.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGLGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSVGLQLDNQALKQANEELTTK
                     AESLAQINSKTMLTSLQNTKDIATIKEQLDGGK"
     gene            complement(2721..3245)
                     /locus_tag="ACGE4T_000400"
     CDS             complement(2721..3245)
                     /locus_tag="ACGE4T_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385714.1"
                     /translation="MVDIVQLKEDGVAKYLKTHADAIDGVDGKLVRATGNETILGAKN
                     FRDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPNGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            complement(3238..4032)
                     /locus_tag="ACGE4T_000401"
     CDS             complement(3238..4032)
                     /locus_tag="ACGE4T_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385715.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKIGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGKI
                     NADVTGFYVLPTASEFVKGGIRVGEGLTMTDDVLSADKQFNYTAGANISISNTGVISA
                     TGGGEGSGVNQEYVDQKMSEAYQNAQAYTDSKIPNFIFEKVGEANG"
     gene            complement(4032..4940)
                     /locus_tag="ACGE4T_000402"
     CDS             complement(4032..4940)
                     /locus_tag="ACGE4T_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFF8385716.1"
                     /translation="MAKWNVVLSTTEPYNYVGMIQVRQGNKNSEVMEATITENGMPYD
                     LTGCKVYFESVVGDKYPVQLGTKVIDAKKGKIQYTFDQYSMQCLHRQTADFIIYKDDE
                     LIATTQDFSYFVIKAVSKTEGEMGSYWQTVEDLIADMSAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGTLIIATVDDPKQNVFTLEKVGVI"
     gene            complement(4906..5424)
                     /locus_tag="ACGE4T_000403"
     CDS             complement(4906..5424)
                     /locus_tag="ACGE4T_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8385717.1"
                     /translation="MIEEKGLNHLKSLLNQPIGNHQCYALSAEYAGVMIGPDMGAGTK
                     YEIKVRHGNVFSAAEIGRAYPWSLYLWTVIAHPEYDQLVVGSIINWERNAKISDTFES
                     HEYYGHTGVIKGLENGRIQTYEQNAESGGIVAEYDREFFGSGQIASICIPPDFEKGVV
                     INGKVERSTQHN"
     gene            complement(5435..8257)
                     /locus_tag="ACGE4T_000404"
     CDS             complement(5435..8257)
                     /locus_tag="ACGE4T_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFF8385718.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNIKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLIGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYSDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTQNVANAKRVWRWNLNGLGYSDNGVNGPFDIAMTSKGEIVADFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNKNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPKEGKGWVILGNGTSILFGSL
                     NLQEKLNAFGDAEFMKNVNIRGKLTINGQEVFPGQGGSGNNDGGSWNGIYPPEVTSQA
                     DKFAWELWVMLLSRGYSKASIAGILGNVQGEAGAAMNPDIAQVGGPAYGIVQWDGSAY
                     PLVPPATWDGRTYVQNLMRAAGITEDYRTMSAQGKLLDWTMYNGQWLGIVQPASVSGF
                     KAMTDPAAAAYTFERNYERPATTHPERQGWAVNWYNRFKDLQVPSAGSILSTAKSLMG
                     YFHYSQPLRWNFGSVENPDRNGYADCSSFVWLALTKAGYKTATRGTLWYTGSMAADAR
                     GARQYLTEISPNEAKAGDIIIVNQGAGAGNDGHTAILAEDWKGYSTSIVEMGGMNSNG
                     VGIGRVDWSFGYLLNGGDVCLVRAKK"
     gene            complement(8254..8958)
                     /locus_tag="ACGE4T_000405"
     CDS             complement(8254..8958)
                     /locus_tag="ACGE4T_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8385719.1"
                     /translation="MNPDEPNFIWKNRNAVIDMDCIIEAELPDIMPNKRYETYTVQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(8970..11273)
                     /locus_tag="ACGE4T_000406"
     CDS             complement(8970..11273)
                     /locus_tag="ACGE4T_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFF8385720.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLGELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSMLQEFSRWWGSLDQDTQNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGINSF
                     IGIWQKMSVFLAANPFVLVIAGIALLIAGFVLAYNKVKWFRDGVNAFFQGISDIAVQV
                     FNFLGGFIGGVFGGIIANFNNYFNAGKRIFNGFLDFITGVFTGNWSKAWEGVVNIFGG
                     IFDGIVAIAKAPINGLIGLINDFIDLANKIKVPKGVPVVGGKGIHFDKIPYLAKGGHL
                     INGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSRGSIEQHIHIGKVDANN
                     PSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            complement(11468..11932)
                     /locus_tag="ACGE4T_000407"
     CDS             complement(11468..11932)
                     /locus_tag="ACGE4T_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385721.1"
                     /translation="MARLADYGIHVDDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            complement(11932..12576)
                     /locus_tag="ACGE4T_000408"
     CDS             complement(11932..12576)
                     /locus_tag="ACGE4T_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFF8385722.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVVRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIAVMDEIKGMIAKSGVTFSKN
                     TAREFPYLAFGFIGNIEGGGKKAVWYPNTQLSNVIDEEYATAEDETKIDDVTANFVST
                     GLKYNNVMYASFDSNREEATMDLFEKFIAQPVYDEAQWKTLIAETTPPETGGGK"
     gene            complement(12583..12948)
                     /locus_tag="ACGE4T_000409"
     CDS             complement(12583..12948)
                     /locus_tag="ACGE4T_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFF8385723.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVEEDRVKVQFEETAFYWRFTENGTTKQKAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            complement(12938..13276)
                     /locus_tag="ACGE4T_000410"
     CDS             complement(12938..13276)
                     /locus_tag="ACGE4T_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385724.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQISFFTLGTEQDLAVIEEALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCMENAK"
     gene            complement(13266..13592)
                     /locus_tag="ACGE4T_000411"
     CDS             complement(13266..13592)
                     /locus_tag="ACGE4T_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8385725.1"
                     /translation="MPKRQTNNLRWKAELLEIKTGTDENDRPTTVYESKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDTREMEL
                     SLAYVD"
     gene            complement(13570..13851)
                     /locus_tag="ACGE4T_000412"
     CDS             complement(13570..13851)
                     /locus_tag="ACGE4T_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFF8385726.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKALSAITPFIVQGVIQNAETTDQ"
     gene            complement(13853..15217)
                     /locus_tag="ACGE4T_000413"
     CDS             complement(13853..15217)
                     /locus_tag="ACGE4T_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8385727.1"
                     /translation="MNKELLKKLKARNEQRLTELRGRIESGEVREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(15230..15802)
                     /locus_tag="ACGE4T_000414"
     CDS             complement(15230..15802)
                     /locus_tag="ACGE4T_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFF8385728.1"
                     /translation="MKKKEIRTFDITNLSTRDVTENNSRIVTGYAAVFNSRTLLWEGL
                     EEVISPGAFSKALSNSDVRCLFDHDWGKVLGRTKSGTLRLEEDERGLKFEVELPNTTD
                     ANNLIESMSRGDIDQCSFGFIPTEETWDYNTDPVLRTVNEVELFEVSIVSLPAYEDTE
                     ATLARSKQEIQQDIAARKKMIQTINDALSA"
     gene            complement(15774..16961)
                     /locus_tag="ACGE4T_000415"
     CDS             complement(15774..16961)
                     /locus_tag="ACGE4T_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8385729.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNVKGGDGNEKEGN
                     SDI"
     gene            complement(17025..18752)
                     /locus_tag="ACGE4T_000416"
     CDS             complement(17025..18752)
                     /locus_tag="ACGE4T_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8385730.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(18749..19201)
                     /locus_tag="ACGE4T_000417"
     CDS             complement(18749..19201)
                     /locus_tag="ACGE4T_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFF8385731.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWSDKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(19313..19693)
                     /locus_tag="ACGE4T_000418"
     CDS             complement(19313..19693)
                     /locus_tag="ACGE4T_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFF8385732.1"
                     /translation="MKYCQFDGCTNKIAKGIYCAEHKRSSKSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(19690..20076)
                     /locus_tag="ACGE4T_000419"
     CDS             complement(19690..20076)
                     /locus_tag="ACGE4T_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFF8385733.1"
                     /translation="MAMIKIRTSITGTEYWDSEKKKTVVVPKGQEPDFEATEEKGTLS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGTIRAKGDIINLIREAE"
     gene            complement(20057..20443)
                     /locus_tag="ACGE4T_000420"
     CDS             complement(20057..20443)
                     /locus_tag="ACGE4T_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385734.1"
                     /translation="MNDFHEAVLTFDVPAGMGQVYKKAIEDDNSRHWIKNEIKDADGN
                     IVISDIKPSWNGNHCSVSIDNSLTIEKYSGAIIDRTERKTHLTIALISRTLPNLKEQV
                     EWYERMGAKVISTNYKGENQNGNDQN"
     gene            20792..20983
                     /locus_tag="ACGE4T_000421"
     CDS             20792..20983
                     /locus_tag="ACGE4T_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFF8385735.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            complement(20976..21389)
                     /locus_tag="ACGE4T_000422"
     CDS             complement(20976..21389)
                     /locus_tag="ACGE4T_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385736.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRASSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(21632..22108)
                     /locus_tag="ACGE4T_000423"
     CDS             complement(21632..22108)
                     /locus_tag="ACGE4T_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFF8385737.1"
                     /translation="MRFQWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSKKYKEQLSLF"
     gene            22468..22881
                     /locus_tag="ACGE4T_000424"
     CDS             22468..22881
                     /locus_tag="ACGE4T_000424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385738.1"
                     /translation="MNKKKQILSNLLIDGFDNANYSHKLLFKSELDDEKEFDKQKDLM
                     CALTYLNQAHASFTNAYTFIALNDELLGGRQEFDNILHQFTVFNTEFLNNVRTNHSHQ
                     WSDIEFKRLVDFFEAASGLLNGHERIQGLINEARK"
     gene            complement(22988..23179)
                     /locus_tag="ACGE4T_000425"
     CDS             complement(22988..23179)
                     /locus_tag="ACGE4T_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385739.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEASQWLLKTYTNNKKLRKKYPDA
                     LLKDDQIMPEPMIFTKWDK"
     gene            complement(23187..23375)
                     /locus_tag="ACGE4T_000426"
     CDS             complement(23187..23375)
                     /locus_tag="ACGE4T_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385740.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMDQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(23372..23947)
                     /locus_tag="ACGE4T_000427"
     CDS             complement(23372..23947)
                     /locus_tag="ACGE4T_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385741.1"
                     /translation="MTKQVNFRPELKKVTSKSNGNTEVLLVVSNGSLRGSTENLTEFL
                     GSTVTVVIQPETIEYTVPVNKQTKKPNIEYVVNSDGTIEMLKEEQTSLDVGDGVEEVE
                     NVTILVSKETIDEFIKTATTLQLPENITVNIRDVLIRLAEGDSMSEIAADHELSEIAL
                     IDQIELARHYFAPYADSWSKHKDDIIFPEEQ"
     gene            complement(23949..24404)
                     /locus_tag="ACGE4T_000428"
     CDS             complement(23949..24404)
                     /locus_tag="ACGE4T_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFF8385742.1"
                     /translation="MATRSKYGNKKHEVDGITFDSKAEARYYMKLKRNGMSFMPLSET
                     YCAMQENVLLQEGYLCNDRKIAPIYYRADFVIYENGQVKKVIDVKGYQDAISMLKMKM
                     FAHRYGFPVTFAKFDSKINKFIEMDCFESARQQRKRQTERRKKKLMEGK"
     gene            complement(24547..24789)
                     /locus_tag="ACGE4T_000429"
     CDS             complement(24547..24789)
                     /locus_tag="ACGE4T_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385743.1"
                     /translation="MTFVVKKMCYLNDRGYGEASLEYADHHETKEEAQLVASVCGGEV
                     VEVIKPERRFSGPKAIPVREEACRPKSNQAWMRGAK"
     gene            complement(24786..25634)
                     /locus_tag="ACGE4T_000430"
     CDS             complement(24786..25634)
                     /locus_tag="ACGE4T_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8385744.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVSGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKTDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRSPV"
     gene            complement(25634..26419)
                     /locus_tag="ACGE4T_000431"
     CDS             complement(25634..26419)
                     /locus_tag="ACGE4T_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385745.1"
                     /translation="MTGGWIKLYRQLLEKPIWQESTPEQKVILITLLSMANHEEREWE
                     WQGEPYKASPGQFVTSLDSIRKKCGKGISLQNVRTALKRFEKYEFLTNQPTNKNRLIT
                     IVNWEVYQSKDNDLTKELTSNQQATNKQLTTNKNDKNDKNDKNDNNPRNSRKTREYAD
                     DDPNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSND
                     FWSGVILSPTSLRKHFDKMAIQKNKRKQQNISNDELPETGEDW"
     gene            complement(26435..27250)
                     /locus_tag="ACGE4T_000432"
     CDS             complement(26435..27250)
                     /locus_tag="ACGE4T_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFF8385746.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKTFTPIIAAVSKQTPSEARLITI
                     DQDNINYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            complement(27213..28235)
                     /locus_tag="ACGE4T_000433"
     CDS             complement(27213..28235)
                     /locus_tag="ACGE4T_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFF8385747.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGENLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            complement(28238..28432)
                     /locus_tag="ACGE4T_000434"
     CDS             complement(28238..28432)
                     /locus_tag="ACGE4T_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385748.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDVILEYVQQHYPKIELGGI"
     gene            complement(28442..28666)
                     /locus_tag="ACGE4T_000435"
     CDS             complement(28442..28666)
                     /locus_tag="ACGE4T_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385749.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            28828..29235
                     /locus_tag="ACGE4T_000436"
     CDS             28828..29235
                     /locus_tag="ACGE4T_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385750.1"
                     /translation="MSFDNTITISIILALVALISPWITAVINNKHAESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKILPILNKSLEKL"
     gene            complement(29349..29522)
                     /locus_tag="ACGE4T_000437"
     CDS             complement(29349..29522)
                     /locus_tag="ACGE4T_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385751.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(29535..29762)
                     /locus_tag="ACGE4T_000438"
     CDS             complement(29535..29762)
                     /locus_tag="ACGE4T_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8385752.1"
                     /translation="MTQELINKNDLDSFLIGYVPKRYLTQKEAVHYTGTSAGTINEWV
                     KKGLKVIIFGENSRPKYDIKDIDEFMSKYKV"
     gene            complement(29836..30036)
                     /locus_tag="ACGE4T_000439"
     CDS             complement(29836..30036)
                     /locus_tag="ACGE4T_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385753.1"
                     /translation="MNRMDTQRIVLETEEKEFIETVISFVTEKGWTISNLQNAVSKVE
                     DYMKKNATLTELTIGMPAQIKD"
     gene            30107..30277
                     /locus_tag="ACGE4T_000440"
     CDS             30107..30277
                     /locus_tag="ACGE4T_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385754.1"
                     /translation="MNKKIAADLVVAWLNHESQISTKGESISPKEIAQAYLDIQYAVI
                     YGQLPEDRKNDD"
     gene            complement(30274..30414)
                     /locus_tag="ACGE4T_000441"
     CDS             complement(30274..30414)
                     /locus_tag="ACGE4T_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385755.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            30708..31028
                     /locus_tag="ACGE4T_000442"
     CDS             30708..31028
                     /locus_tag="ACGE4T_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8385756.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            31046..31477
                     /locus_tag="ACGE4T_000443"
     CDS             31046..31477
                     /locus_tag="ACGE4T_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFF8385757.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            31604..32188
                     /locus_tag="ACGE4T_000444"
     CDS             31604..32188
                     /locus_tag="ACGE4T_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFF8385758.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            32302..>32681
                     /locus_tag="ACGE4T_000445"
     CDS             32302..>32681
                     /locus_tag="ACGE4T_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFF8385759.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTV"
BASE COUNT         9157 a         6863 c         5309 g        11352 t
ORIGIN      
        1 cggaaataag ctgaaattca caaaaatttg aaaagcaatt tccgtgaatt tcttcttatt
       61 tcttggagtt aaacgcttct gttccagcct ctttttatct gtctttcact aatgatcagt
      121 tgtagataag caagttgctt tttcttacat cataccgccc atcaagacaa atgtttttag
      181 cttgtacgta ctggacagaa atcccgttat atcaaggaaa aacaacgttg ataatatttg
      241 aaattattag gctagaacgg actagtaaga gttttttgtc ccttttttgc cccctctacc
      301 ttaaaaacat ttcttatgta ccctgtagga ctcgaaccta cgaccggacg gttatgagcc
      361 gtctgctctg accaactgag ctaagtacgg aaagccataa acagtcaacc agtagaattt
      421 gtggcaacaa acctgttatc gcatatcttg gagtgtgact atttatgagt gatagtgaag
      481 atatgcggca acatcactat tttatcgaat gatttttata gttttcaata taattatgta
      541 ccgcccctca tcgaggggct atttttttat cgttgaggaa tatttaaata ccaacgcttg
      601 tcatgaaaat cttgtgctcc gcctttagtg ttcccttctg gatcattagt tgcccgcatc
      661 ataacgtata ctttcttgtt ggggaaatta cgcatgttga acgatacatg ataaccaacg
      721 tttccataag tactataaga ttggtttaca tctggacgtg aaacgccatt agcatttact
      781 cgtgctaact ctttgccagt attatagtcc atgataaaga tatactcata cttataatta
      841 gcgatgtgcc atccagccac atgcaagttt gcgttttcga tttccccaaa ctgatcaatg
      901 tgggcgtaat ttgttccatc tgtcagcgta ggatttgctg cacctgctct agttggatca
      961 atgactggtt tatcatctga agtagttgga ttttcatcgg taaaaccatg agccaaatca
     1021 taggctagtt tttctttact tacacccatt tcagaaagat aaccgtaagg atctgtatga
     1081 tcgccccaaa tattttgtgt tacccataaa tgcgatttga ttcctggttg gttataagga
     1141 gtgtctaatg ttaatggaat accatatttt attgctgaat ctctagccaa ttcaacatat
     1201 gctttatagt tcttttcaaa agttgcttta tcatgtgtgt gttgtaactc aatctgcaca
     1261 ggactgttag cattagcata cgaaccagca ccgtactgta cataaccagg ttgaccgact
     1321 tgataaacaa ttccgccgtc tcccacaatg taagcagtat aagcactagt ccatgaacgt
     1381 tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc gtgcagtaaa
     1441 atgtactgat tatttgctac ttgtgagcta ccttcgtttg ggcccaaatt aaattcattg
     1501 ttaatcgtat aggcaaaccc attaattggc aataaaaata gagccattaa taggctcatc
     1561 gcagtaacag taattttctt tttcatttgt ttcctcctat tttttcaaat tataagccga
     1621 cacaccggtg ataacgccta aaaatgttgc tgctgcattg atagtcagaa ctgtcatatc
     1681 tgttccattc catccatatg cttttcctaa cgtggctact aacacagaag cagctggcaa
     1741 tactgttaaa accgtccatt taatgacttg ataatactta tcgggcaaaa tcatttcttc
     1801 ttaactcctt tcttttttac cgagatcttt ctctaaataa agttttaatt tgttccgtgt
     1861 gttccaccaa tttttctgca tgtgtatcta atcttccatc atgtttcttt agttcttcat
     1921 gaatagtaat gcgatcagat ttgcttgctt ctaaatcttt agtcagtaaa tctaaatttc
     1981 ggcttacttt tgaaagagtc tcagtaatct tcgagaaaga tgcagtaatt ggtcttatta
     2041 ctaataaaat caaagaaaca atcgcagtga ttgatcctgc tatcattccc cattccccta
     2101 aattaatcat gtgacaactc ctttacctta aataaaaacg catcaattaa gatgcgctct
     2161 tatctttatt aatgatttta tctgcttctt cgtctgtaat gcatagtgga acgaattgtc
     2221 gaacttggtc agcagtaaaa caattccagt catacatcat tttgatatca tcataagaat
     2281 acatattatt ttcctccgtc aagttgttct ttgatagtag caatgtcttt tgtgttttga
     2341 agggatgtca gcatggtttt cgaattaatt tgtgctagtg attctgcttt tgtagtaagc
     2401 tcttcatttg cttgtttcaa tgcttgatta tctaattgaa gacccactga aaggttttct
     2461 agtagttcca attttttcga ataatcttgc gtaacagctt cttcccactt attttcagtg
     2521 aagttaaaaa attgcgactg ttgtcttctt ccgagacctt catccgtttc atcttccttc
     2581 ttttcaaaca aaataggtgg aacttcgaca aatggtaaag atgttggaaa gttatcttct
     2641 acttgatgtt cttcatatcc cattggatac aatactttat aaattgtttt cattttactc
     2701 ttcctccaat tagtaatctg ttaagccggc caaggatctt gagtgatcca catacctgaa
     2761 atatatgaac tacctgaacc tgatttggct tgtatgatgc tactttgatc aatgaatgca
     2821 cgtgcatccg ctggctgact tgcatttcta actagtgaaa gcgcggtttg cgctggatag
     2881 ccttgatcac gtttgaaacc gttcgggatt tttaaaatcc aacgagtttc tgatccttcg
     2941 ggccatgtgc cacatttaaa attaaaagta agaaatacga tgtttccgat tctgattatt
     3001 tttccattca catcagttac gtttgttgta tcacttcgat cagctagtgt gatagctcgc
     3061 tcgatcatac ctgcttgcac aggcaatcca ttgaactgta atccatcacg aaagttttta
     3121 gcacctaaaa ttgtttcatt cccagttgcc cttactaact taccatcgac accatctata
     3181 gcatccgcat gagtttttaa atatttggca actccgtctt cttttaattg aacaatatca
     3241 accatttgct tctcccacct tttcaaatat aaaatttggt atttttgaat ctgtataagc
     3301 ttgtgcattt tgatatgctt cactcatttt ttgatcaaca tattcttgat taactccgct
     3361 accttcacca ccgccagtag cactgattac ccctgtattt gaaatactga tattagctcc
     3421 ggcagtatag ttgaattgct tatcggccga taacacatca tcagtcatcg ttaaaccttc
     3481 cccaacgcga ataccacctt ttacaaattc agaggcagta ggtagaacat aaaaaccagt
     3541 cacatctgca tttatttttc catcttcatc aatagtgatg ttttctcctg cagtgtaatt
     3601 tttcagtgaa tcgagttttt cttttaactc cagcgaaaaa ttgacatcag attgtctgac
     3661 tgcagagact tttcctgtct catcaatcgt caataattca ccaattttta tgccacccaa
     3721 acggtcgata gtggcaactg gcaacacgta gtcaccttcc ccactgttta aaattttttg
     3781 atacatttct gccgtgataa tgccgtcatc ttcttcactt gcatagggta attctgtgag
     3841 aacattctct aatcccaaat cttctttagt aatgacgacg gctccaattt ttccattaac
     3901 agataaaact gttgcttctc ctgctattat ttcttctaac ccaagaactg cggaagcatg
     3961 tgtgattgga aagaactggc gttgtacacc tgttttttca tccgtttcca tcattcgttt
     4021 agttttagcc attagatcac cccaactttc tcaagagtga atacattttg ctttggatca
     4081 tcaacggttg cgatgatcaa ggttccttct tcaatcggga attctacagt tccaactttt
     4141 tcgacttcgt gattatctga aaagagatca tcctgcaaaa tatcagtcac ttcaatctcg
     4201 ccgtattcaa tcgtgaaaag tgttgctcgt aatttctgat atagataatc catatctgcc
     4261 agcaagcgct ctgaaataga cgcatgacga actccttgaa tatcaacacg tgcatccatt
     4321 aattcggcta acattactcc acctggatcg atagacttca aaatatcttt gattgattca
     4381 aaccatttta aataatcggt ttcttgtcct tctctccatg cctcaaatgt attttgttgg
     4441 tcttgtcgcc attgttcaaa ttccttttta cgtgcattca tccaatctgt gaagtcacct
     4501 ttgttttcat tgataaaagc tgacatatcc gcaattaaat cttcgactgt ctgccaatac
     4561 gatcccattt caccctctgt ttttgagacg gctttaatca caaaatagga gaagtcttgc
     4621 gtcgtagcaa ttaactcatc atctttataa atgataaaat ctgctgtttg gcgatgtagg
     4681 cattgcatcg aatattgatc aaatgtgtac tgaatttttc ctttttttgc atcaatgact
     4741 ttagtaccta attgaactgg gtatttgtct ccgacaactg attcaaaata gactttacaa
     4801 ccagttagat catagggcat tccattttca gtgatggttg cttccataac ttcggaattc
     4861 ttattgcctt gccgtacctg aatcattccg acatagttat aaggctcagt tgtgctgagt
     4921 actacgttcc actttgccat taattaccac tcctttctca aaatcaggcg ggatacaaat
     4981 agaggctatc tgaccagatc cgaagaattc acggtcatat tccgccacaa ttccacctga
     5041 ttctgcattt tgttcatagg tttgaatacg cccattttcc agacctttga ttacacctgt
     5101 gtggccgtaa tattcatggc tttcaaatgt atcactgatt tttgcgtttc tttcccaatt
     5161 gataattgag ccaacaacta gctggtcata ctcaggatga gcgatgaccg tccataaata
     5221 caatgaccat ggataggctc gtccaatttc agcagcggaa aatacattgc catgacgtac
     5281 tttaatctca tatttagtac cagcccccat gtcaggtcca atcatcacac cggcatattc
     5341 cgcagataat gcataacatt gatgatttcc gataggttga ttcaacaaac ttttcaaatg
     5401 atttaatcct ttttcttcga tcacaaaatc acctctattt cttcgctctg acgagacaaa
     5461 catcgccacc gtttaataaa tacccgaagg accaatcgac gcgaccgata cctacaccat
     5521 tggaattcat accgcccatt tcaacgatag acgtgctata tcccttccaa tcctctgcta
     5581 aaatagcagt gtgtccatcg ttaccagcac cggctccttg gttgacaata atgatatctc
     5641 cggctttcgc ttcatttgga gatatttcag tcagatactg acgtgcccct cttgcatctg
     5701 cggccattga acctgtgtac cacagagtcc cacgtgttgc ggttttatat cctgcttttg
     5761 tcaaagccaa ccacacaaaa gaagaacagt cagcataccc attacgatca ggattctcga
     5821 cgctaccaaa attccaacgt aacggttgcg agtaatggaa ataacccatc aagcttttgg
     5881 ctgtactgag aatactgcca gcagatggga cttgaagatc tttgaatctg ttataccaat
     5941 taactgccca tccttgtctt tctggatgag ttgtggctgg tctttcatag tttcgttcaa
     6001 atgtataagc agcagctgct ggatcagtca ttgctttaaa tccagaaaca cttgccggtt
     6061 gaacaattcc taaccattga ccgttataca ttgtccaatc taataacttt ccttgagctg
     6121 acattgttcg ataatcttct gtaattcctg cagctctcat caggttttga acataagtcc
     6181 tgccatccca tgttgctgga ggaaccaaag gatatgccga accatcccat tgaacgattc
     6241 cataagctgg tccgccgact tgtgcaatat ctggattcat tgcagcgccg gcttctcctt
     6301 gaacatttcc aaggatccca gcaatggatg ctttggaata ccctctagaa agaagcatga
     6361 cccataattc ccaagcaaat ttgtctgctt gactggtaac ctctggtggg tatatgccat
     6421 tccaactacc gccatcatta tttccacttc caccttgtcc agggaatact tcttgtccgt
     6481 taattgtgag tttcccccga atattgacat ttttcataaa ttccgcatca ccaaaggcat
     6541 ttagcttttc ctgtagattt aaacttccaa ataatataga agttccgttc ccaagtatta
     6601 cccaaccttt tccttctttt ggtgaaatga gaatatattt cccatcgcca tttgttcgaa
     6661 tcactaacga attatcttct aacggtgtag gagtggatgc gccaggaaaa ggattgcctg
     6721 cagaatcagt cgtaccgatt gtgccgatgg attctttggt gttccaaaac tccatccctt
     6781 ttttggttaa ttccatgatc tttttatttt tattccaaat ttgtaacgta cctttgacaa
     6841 tttttagcac atcacctaga tcattaattg agctttcaag aacttctgca ttaataatcc
     6901 caactttaat gaaatcagct acgatttcac ccttcgacgt cattgcgatg tcaaacgggc
     6961 catttactcc gttgtctgaa taacctaagc catttaaatt ccaacgccac acacgcttcg
     7021 cgttcgctac attttgagta tccataataa gaatctctga tggagctttt tctggacgaa
     7081 aaacgacatg cccaccagaa ttaccagtga tccatgccgt cgcattcaat acattttgaa
     7141 ctaacgtttc tgttcgattg tcgattttct ttttcaattc ttgtgcttga gtatttactg
     7201 cagaggtgta cagtgataaa tcattaccta aaacaatatc cttatatttc ccaagcgttg
     7261 ggaaataagt gtattcgacc atacgctctt ttatctcgat atctagctct ttcgccctaa
     7321 catgagcgac atcaccaaag tgtaaagacg ctagctcttg atacatgtca ctgtattcga
     7381 gtgtgtgttc caacgccacc atactgactg tatgagtagc ttttggctcg tggatacgat
     7441 cattgtcaaa taaggtttta ccccatttga tcaattcatc aacggtttta caatcgccat
     7501 tttcacgttt ggcgatacgt cgattctcgt tggtcactcc atcaatttct aaatatccat
     7561 attcaatagg ttctttgtct tcatcataat cgttatcagg aaccccgcca atcaaataca
     7621 ggctatttac gatggattct tcatctattt cttcttcgat tgcttctaaa ttgataccaa
     7681 aatcgattcg gaaaccatta tctgatccaa tctgttttac cagtttcaaa tcatagttat
     7741 ccatatcaag ttccgcgcta gtcacgccag tcaaattttg attaccgtta tttgagccga
     7801 taatcgcttc aactggagca acttgtcttg ctgtaaattg gtgtgttgtg cctacattcg
     7861 acagatagtt gaatttttga tcaaatgcca aactgttttt aagattggtc atgatctgag
     7921 aaccgttgcc gttatccgtg aatgacttaa cgataaagtt tttattggcc ataaatccaa
     7981 tatgacgagc agttacagaa acagacgtca aattcttttt gatattgtaa atttcgaagt
     8041 attgccatga tccatctggc accttcgctt ttatgaaatt tccttttttt aagtaagacc
     8101 gatactgtcc gcttcttgaa tagttaccat aaaaacgata ttggccattc aacacacgat
     8161 tgatttctgg caaatcttcc cagtcaacta acgacgctcc gttcacgctc aaatcatctg
     8221 gcattttttt ataagcataa ataaattctt gcgtcacaga tacacactcc tattccaaaa
     8281 ccgcacttct ttaaattttc cggatatttt tacctgattc cattcaggct gcaatactgg
     8341 ccaatcacca cgcgtaaata aattcaaacc ttcttgtatt gcttttccaa gctgagtatc
     8401 aaccacgatt gttgcagcta acgtattaag tattgttagg cttttatcac cgacagcgat
     8461 cgttatatct cctccgttcg attctatttc cagataggga tgtgcaattt cgtcaccatg
     8521 atcaaaaata tccataacac tagaatgaaa aattttaggt gcttcaccga tttttctttt
     8581 gagtggctga caacgaaaag taacatcgaa tgtataaaag aagccccact cgttttcgaa
     8641 agggacttct ttatccatgc tgcagatagc atctagatac ttgtctggat cattatgagt
     8701 gattaattgg cttttaccag taagccatct tttgacttcc ctcagtttgg aatgtgggat
     8761 agtgatccct tctatttcta aatcaaaagg ttcataatca tcaaacgtct ccgtcaattc
     8821 tccgcttcgg ccctgaaccg tgtaagtttc gtatcgtttg ttcggcataa tatctggaag
     8881 ctctgcctca atgatgcaat ccatatcaat tactgcattc cgatttttcc aaataaaatt
     8941 tggttcatct ggattcataa attgtcgact caaataggaa cacctcccaa atcataaata
     9001 gcctgttggt ttgctttagc aaatttacga ttcatacgat ctaactcaga tggattgttt
     9061 gcatcgactt tgccaatgtg tatatgctgt tcaatgctac ctctggaaac tcttccactg
     9121 atccctttgc gtttttcttc gtctgataat ggagtaactg tggttttacc attcttggca
     9181 gtcagtaact caggtccagc ttcaccaaca attgcttgcc cattgattaa gtgaccgcct
     9241 ttagccagat aaggaatttt atcaaaatga attccttttc ctccgacgac tggaactcct
     9301 tttggcactt taattttgtt tgctagatca ataaaatcat tgatcaatcc aatcagaccg
     9361 ttaattggtg cttttgcaat tgccacaatc ccatcaaata tacctccaaa aatattcacg
     9421 acaccttccc atgctttcga ccagttacca gtgaatactc ctgtaataaa atctagaaat
     9481 ccgttaaata ttcttttgcc agcattaaag tagttattga agtttgctat gattccaccg
     9541 aatacaccac cgataaagcc acctagaaaa ttgaatactt gaacagctat atctgatatg
     9601 ccttgaaaga atgcgttgac accatctcga aaccatttga ctttgttata agccaaaaca
     9661 aatccagcaa tcaataatgc tataccagca atcactaaga caaacggatt tgcagcgaga
     9721 aacacagaca tcttttgcca aattcctatg aaactgttga taccaccgac aatttttgta
     9781 acagatccca taagtgaacc aagaacgatt agtactggtc caactgctgc cgctatacca
     9841 gcaattgtta taatccaatt ctgtgtatct tgatctaacg acccccacca tctcgaaaat
     9901 tcttgtaaca tagaagttgc tttttcaaaa aatggcaaaa ggctgacttg tactgcttcg
     9961 cctacgtcgg ccattgcgag ttttgcgttg ttcatcgctt gatctgcttg atcaattggg
    10021 tcgagagtag catcgaatgt gtctcctaca gctcccccac tttctccagc agtttttgct
    10081 aaatcttcca ggttcaaagt acctctacga attgcatcgg ccattctagg accgccttta
    10141 gtcccgaata cttctgctgc tgcattaata gcttctgttt ctgaacctgc attttttacc
    10201 ttgtcttgca attccccgag accctgactt aaagattttc cgtctttcgc ataagccact
    10261 gttgctttag ataaactact taaagctgcg cttgaatcaa caccagattg ctcaaattgt
    10321 ccaagtaaag tgaccccttc accaaagctc aaccctaatt gtttaatttg tggtgcgcca
    10381 tcaattgctt tttgcatcaa gtcgtctaca gattgaccag tattctgcga tgttttcgtt
    10441 gttacatcaa ggacagaatt caaatcatca tattctagtc cataagcatc aatagcttgt
    10501 cgagcagata ttgctgattg cgaaacatct gtatcattga tttcagcgta ctgtaggaga
    10561 taattggttg aatcttccag ttttttatcc atgaatccaa attgtgtatt tacctcacca
    10621 atagcttccc caaccgtttg taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac
    10681 tgtgaaagtc tatcagcttg atcacctgtt gctccggttt ttgtaatgat ggtatcaagt
    10741 gcctcgtcaa cttcaccaaa tgctgcaagt cctgctgctc ctgccgctac aataggcgcg
    10801 gttactccaa cagacatttt ttcaccaacg ccttttactt tctcacctgt ttcttcgatt
    10861 ttctggagtt tttttgctgt atcaacagaa acgtcacctt gttctttaag tgcatcgttc
    10921 gtctgttcta atgctgtacg aagtttattt tctccggttt cagaattgag taattgttta
    10981 taaagttttt ctgattcagc agaatattca ccggtttctt tgacagattt ttcatattcc
    11041 tctctcagca tttgagatcg ttgttcagcc aatcctaatt gtttttctaa cttttgttta
    11101 gccgctcgca atttttctgt ttgagttgca tccttgtcca tcgcagacac ttggtttttg
    11161 tactcggcag cggctaagtt catttctttg ttgatttctt tgattgtttt ggaataactg
    11221 acttcgccat tcgttttaaa attaagaacg acatcagatt ctttaccagc catttatctt
    11281 agcgctcctt tctaccacca aggcgattta tccatagtta cagattgagg tggttcaaaa
    11341 tcggtgtttt gttgtaacca ctgtaaataa gatttgagcc acaagttagg tgtagacttc
    11401 aaaaagaaac tctcactcca attcaataga gtgagagcaa cgtaaatata aaaactccaa
    11461 ggagttccta tctcttccga ttctttttgt ttacctttct ttttgcttgc gttttttgaa
    11521 agtcttgtgg cttcttggat ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa
    11581 tttccataca ggtactataa gcagacaaca cctctccact cattcccaaa aatttgaaaa
    11641 tagtttctgg atcttcctct aaaccaccag tacgcagcat ggcataaatc aaggcacgca
    11701 tgatttttag atcactaggc gataagttag cagaagaaat ttgtccttct tttttagata
    11761 gcatggcgtt catatcagat tcaaattgcg aataatctcc accatacaca tctgctataa
    11821 attccatcgt ttgcatagta aatgaaatag ggaattctgc gccttgaata atgacaacag
    11881 cagaattctt taaatcatca acatgaatcc cataatcggc aagtctggcc attatttgcc
    11941 acctccagtt tctggtggtg tagtttctgc tatcaacgtt ttccattgtg cttcgtcgta
    12001 gactggttgt gcaatgaatt tttcaaataa atccattgtc gcttcttcac gattcgaatc
    12061 aaaacttgca tacatcacat tattgtattt cagtcctgta gaaacaaagt ttgcagttac
    12121 atcatcgatt tttgtttcat cttcagcagt agcgtattct tcatcaatta cattagatag
    12181 ttgcgtgttt ggataccata cagctttttt acctccgcct tcaatatttc caatgaatcc
    12241 aaatgcaagg taaggaaatt ctcgtgcggt atttttacta aatgttactc cgctttttgc
    12301 aatcatccct ttgatttcat ccattaccgc aattggaata cctacatgat ccaacgcaat
    12361 ttcatgtttt gtttctcggg acacgcgtcg gaacattttg cttgatgccc atttttccaa
    12421 cgctgtacca ttgcctttga ctccaatttt tgtagcgata ggcaaccgga caacttcact
    12481 ataagtcgga gcagttccaa cagaatctgg cgttgccatc atcgcgatta aaatgtcgtc
    12541 caatccttca aaataaaaag tatcttgctt tcccaattaa aatcatcctt cccataaatc
    12601 aagtattttc ttcgtcatga tctcttcgat tttttcttta ttttgttcat acgtaccgct
    12661 tgcaaagtgt tgtgcttttt gttttgtcgt accattttcg gtaaagcgcc agtaaaaagc
    12721 agtctcttca aactggactt tcactcggtc ttcttcaaca acaactttta tttgttcgct
    12781 catatgcttc tttttaagca gggacttagg aatattagga agcaactgct ctaggtaaaa
    12841 ctttgcagct tcttctaaag attccaatga taattttttt ggatcaactc tagaaagatt
    12901 ccctaaataa tccgatattt cagaaaaacc attcttatta cttggcattt tccatacacc
    12961 tcacatatgt gtaatagttg gttacggtat cgtcgttctc atcaccttgt atacctgtaa
    13021 aatctgaata tggaatacca gcattttgta acgcttcttc aataacagct aaatcctgtt
    13081 ctgttccaag tgtgaaaaaa gaaatttgat aatacggtaa ttttttatag actttaccgg
    13141 atgccatttt tttactgtta ctcacatttg agtacacgat gtatggatac atcgtcccta
    13201 atctggcttt atctctgaac actggcaact ttgttgattt cagcgctgtt ttcaattcat
    13261 caaagctaat cgacataagc taaactcaac tccatttctc ttgtatcagg attcgtataa
    13321 atgcgagtaa tgttatacgt tacagaatca attttgagcg cacttaattt ctctgtgatg
    13381 gatttatccc atctgacttt aattcgtctg acaacgtctg tcttggcttg ctgtgataaa
    13441 tatttttctt gagaagtcac accgagttct tcatagaata ttagacgctt ggattcataa
    13501 accgtagttg gacgatcgtt ctcatctgtt cctgttttga tttctagcaa ttcagctttc
    13561 catcgcagat tattggtctg tcgtttcggc attttgaatc actccttgta caataaatgg
    13621 cgtaatagcg ctcaaagctt tgtctaactc atcttcagat actcggtatt cgtaggcaat
    13681 gccggcaacc atcaaaataa gatattcttg ttggccacca gttgctgttt tgacataatc
    13741 ttttgccata tttaaataaa aagagagcaa agaatcatcc atgccctctt caaaatgaat
    13801 atgtgatttg aatttttcct ctaaagacaa ttctttagtt tgctcttcca tcttaaccac
    13861 caactggttt tgtaatttcg tagcgatata ctgccggttc aaatggagaa taaaccaatt
    13921 gaccatctag caagttgtaa atttggaatc cgatttgatt tttaccagag aatttttcaa
    13981 caagtttttg aatttccaag gcaccaataa cttcttgaat tttaaatgca gaaaaatcgc
    14041 caaaatataa aactggtgtg tctggttcac cctttttatc tgctgcatct gtccaatcca
    14101 caggatagcc aactaattgg taacctattc caccttctgc ttgtgtaaat ggacgcaaca
    14161 aaggaaatcc atcatctgtt ttcatttttt caatagcagt caaagcagct cgattaataa
    14221 taaagcgtcc ctttttcatc acttctgtca ctggtgtatt tttaaattcg attaatgcat
    14281 catataattt ttgcccagca cctgcagcag ttagatctaa aggtttttca aatgctacag
    14341 cctttttggc taatgcacca ggattttcat ttccagcgtc atcaccattg aacatataat
    14401 tgatttcttt acgcacataa gcttttttca attcttccac aacaatatct tcaactggaa
    14461 caccagacat ttttagtaat tttttagtta ctgttgccaa agcatcgaat tcggcaggat
    14521 caagcaaaat ttcatcaaac tgaatagctg tttcagcaat atcagttgaa cgctctttct
    14581 tgtttacatt cgcatctgct ttcttcacaa gaattggata tttgacatct cctgatgttc
    14641 gcaccactgt tccgtattta cgaagtaaat tttcttcttg agcataagta ataacttcag
    14701 atgcaattac ttctgggaca gtaactgagc cgttgccagc ttcaatccct aaagctcgag
    14761 cttctgcttc agaaatattt ccaactacaa aattagcaaa tgcttttcgt agttgttgat
    14821 ctttttgttt attggtcatt tttgcacgtg cctccaatcc gttcttaatt gatccaagta
    14881 atccatctcg ttgctgttga gtaatcattc cagaacgatt ttctgaacta tcttcactgt
    14941 ctgaatcttc ttcttgatcg ttgtcttcac cagaacggct ttcgtctgaa tccgtactat
    15001 tcgattgatc atctgtattg tcgtctgttt catcagttcc agaatctgcg cctaattcgt
    15061 ctttaattcc gttcaattca tcaataacac tgtcaatttc ttctttcacg gcttctaaat
    15121 ctgcttcacg cacttctcca gattcaatcc gtccacgtaa ttctgttaat cgttgctcat
    15181 tgcgagcttt taattttttc aacaattctt tattcattag tttttcctcc taagcgctca
    15241 gcgcatcatt aattgtttga atcatttttt ttcttgctgc aatatcttgc tgaatttcct
    15301 gtttacttct agcaagcgtt gcttctgtgt cttcatatgc agggagtgaa acaatagata
    15361 cctcgaataa ttccacttca ttcaccgtac gcaaaacagg atcggtgttg taatcccacg
    15421 tttcttctgt tggaataaaa ccgaagctac attgatcaat atctccacga ctcatagatt
    15481 cgataaggtt attagcatcg gttgtgttgg gtaactcaac ttcaaatttc aacccacgtt
    15541 cgtcttcttc aagccgaagt gttccacttt ttgttcgtcc tagaactttc ccccagtcat
    15601 gatcaaatag acatcggacg tccgaatttg ataatgcctt cgaaaatgca ccaggagaaa
    15661 ttacttcttc taagccttcc caaagtaacg ttcgactgtt gaacacagca gcatacccag
    15721 ttacgattcg actattattt tctgtaacat ctctagtact caagttggtg atatcaaatg
    15781 tccgaatttc cttctttttc atttccatca ccccctttca cgttttgatc attcgttggt
    15841 aaggaatcat ctgttgcatt tttctggcca atcctagaca agtcatttga aatatagata
    15901 gcttgtgttt ctggagtgtt ctgtttagga aaaccaagca tttctgccac attatctgga
    15961 cttgtaatcc cagtacgaac aatgttgtag ccaatatttg tttttgttga gtaaggaaca
    16021 aaatccaaaa tgttaatttt ccattccact cgatagccag aattaggcat aaaaaaaaga
    16081 gcggtgtaat gttcgctctt gttcttcaat attggtttga ttgctttgtt gtgcagatac
    16141 atcatcgctt tttcgatgtc tgttttcatc aacgattgat acgtatcaac attgattcct
    16201 aaaaatttac ctaagtcttt tttataaaca cctaaatagt tcaaaatagc cgcgtcatca
    16261 acaggacttt ttaacgtctc gatggaatat ccttttccca gaggaatcat cttaacagaa
    16321 tgatcactgt cattttgcgt tccttccagt tgatccaata tagctttgac aattttcgtt
    16381 tgggcgctat tatttggatt gatgtgggcg tccagtttaa gcatgaacgc gagtaaacca
    16441 cctttagtat atttgtccgt caaaactttt tcagcgctca gaacgccttc cagagtgttt
    16501 cttgcaaggt caattattcc agcacctttt aatgaatcag ttccgatgtt cttaatatgt
    16561 cgaatcattt gaccaggtat tttttgtcca ttcatttcaa attcttcttg aagtcgttca
    16621 ttgattttag tggttacacc gtatgccaaa tgaagctggt cccgatctgt taatgggaat
    16681 gtctcaccat tgatcagtag ggtatttgtt tccaatttgg taaattcgaa tccggtcaaa
    16741 taatcattgg gattcttcaa aatttttagc aagtggtggt ccttcacttc attaccgtct
    16801 ggacctatga caacaggtga ggacaacgct acctggtttg agatatcctg gaccaattca
    16861 taaacatcag aagattccat gatagaggaa tctgttacat atctttgacc gtaacgcgta
    16921 tagtggccaa acatatcctc gatgtaccca cgcttttcca taaaggaata gactgcattc
    16981 gataaccgat cacgtaattt caatatttct caccgccttt ctatttatct atagatggaa
    17041 ctaaggtaat catctaattc atccgaatca atatctgtca tctgattcat cgtttcctta
    17101 tgaccacaca aaaacgccac gaaaccatca atctttttct ttgattgacg tttacttggc
    17161 gctttttgtc cgttgatgtt agtgattgct acaacgttca aggtgcaata aaggaacaat
    17221 ggattatcaa attgaattcg tttctcataa aacaaccgtt cgacatcatc aaaaggagcg
    17281 ttcaacactt taggatattg ggcaacctca acgcattcca atcctaagtt ctccaatttc
    17341 tcaacaagtt tgtcgctcat cgctggatca taattcactt gttgtatatc gtataaatcc
    17401 atgcagtctt cgatgtactg caagatttga tcttgatcaa tcattttacc atcgcaaaat
    17461 tcaacaaaac cttgttcagc taaatcgctg taaggcacgt tatcttcttt ttctcgaaac
    17521 tctaaatctt cattgggaat aaaataaagc tgcttcactt taaggaccgc ttttccatct
    17581 tcatcccacg ttgggaagtt taaagatacg caggtcaaat ctcgtgtacg tgataaatct
    17641 aaaccgatat aacatggttc gccacttaaa tcaccaagtt cttgtgtggt aactaaacac
    17701 ggctctactt gatcctgttc aaagaaatta tccgcgccgt tcacaaatac atccaagtgt
    17761 ttcgttagaa attcagcttt ggaatgagct gaccgttgcg ctgttttaaa tgccgattct
    17821 aaagcagata aatcaactga tattccccag ttaggattgc acatttccca aacttttcta
    17881 tccgtccaat catagttttt atttggttca tagattagaa caaagttcga atcattatca
    17941 tcacgtttca acacttcttt tgcttcacga tatacgcgca ttccaacaga cgatgaacct
    18001 ttcccagcag ttgaaatatt aaacatcaat ggttgtggca aagagatttg tgcagattta
    18061 aaattgtcat actgttccat tttctcttgc ttatgcagct catcattcaa tacaaaatat
    18121 ggattagaac cctcaatgtt atcgatattc ttcgtttgaa caataaattt attcgaataa
    18181 gccatatctt catgaatata atcataagta atactagaaa cagttccctt tggtcctttg
    18241 aatattttag ttccttcaag taggattgga ttgtttaata ttgtagctgc aaaaggtttg
    18301 gcagcatatt gagcttgagc aaagtcagaa gcgcatgcat aacagtcgac tgacaaagca
    18361 ccttctccat acattgcata gcctaatgaa ccgacagcaa tcaatgtttt accgttcttt
    18421 ttagggattt ggacatacgc ttcacgagta acacggacaa tttgcccttt ttcattttct
    18481 ttaacccatc catacatcca cgagtaaata aatttttccc aaggttctaa aatgaatggc
    18541 tttccaacca tctcaccttt tgtgtgaaca ataaacgact caacccagtc catcatttca
    18601 ttcgcacgat ctacatcaaa ccaaatatct ttacgttttt tccaccgata ccaacgatcc
    18661 actgccaaac gaacagtttt aggatatttc ccaggtttct ttcttacttc ttttgcaaat
    18721 aaatcggcat aatttacacc aggttcgatc atttttcagt acctgccttc ttacgccatt
    18781 tgtttctgtg cgctgctagt tcatctactg gcttttcttc tggacgtgta atttcttcat
    18841 cttttcttgc agtcgatccg ccagttattt gtctaccagt tttagcctta ttcgttagcc
    18901 ccaacaaatc tagagctttc attttcttat ctgaccaagt ttctacttgc tgcgccaatg
    18961 gatgcttcga ttgattagtg gcacctgatt tattcgtgaa tttttgcgtc tccggaaagc
    19021 ctttttcctt ccacaaaaga tatttgtgtt ggtaaatttc aaaaatatcc aaatatgatt
    19081 cgattaatgg atcaagagtg atggtgtaca aatcagacaa attcattatt tttaaaatac
    19141 gagctttttc agcacttact ttttcatcaa caatcgcttt acgttgcgct ttagtcgtca
    19201 tacttgtata cacccccctt tgttttttga aaaatttgac ctaacgatgc gcgtgactcc
    19261 ccgctaccct atctccccac gcgaaaaaat tttgaaaatg gatagggggg cttcaattga
    19321 aatacgaagg gaaaactttt ttgtcttccg tttcattttc aacaattgga tgacattttg
    19381 aacataaaag catgagattg tttggatcaa gcttaagcag ttcattgtct ttgatgggta
    19441 caatgtgatg gacatgtgcc ctcttgccga agataaactg accgcatcgc tgacagtgac
    19501 caccttctct ttcataaata aattgacgca tatccttcca tgcctgtgtt cgatagaatg
    19561 gtttgttctc atgatgataa acagactttg cttgttgctt cttcttacgt gatttgcttg
    19621 atcgcttgtg ttcagcacaa tagatcccct ttgctatttt gttcgtgcat ccgtcaaact
    19681 gacagtattt cattctgctt cacgaataag atttatgata tcgccttttg cacggatagt
    19741 accaggaata tcaataccat gtttcttagc atatgcacgc aattcttttg cagtcatatt
    19801 gtccagttca tccgtatctt cagtggactg atcattagta gcttcgtctc catcaaaatc
    19861 agcagcagtg tttccatcgc tatcaagaat ttcactgcta ttagtaataa gttcaccatt
    19921 aacagccaca aacgttttac catcacttaa tgttcctttt tcttccgttg cttcaaaatc
    19981 aggttcttga cctttcggaa ctacgacagt ctttttcttt tctgaatccc aatactctgt
    20041 tcctgtgatg gatgttctaa ttttgatcat tgccattttg attctctcct ttgtaatttg
    20101 tactgattac ttttgcgccc atccgctcat accattcaac ctgttctttt aaattcggta
    20161 aggtcctaga aatcaatgct atggttaggt gagttttgcg ttctgttcta tcaattattg
    20221 ctccggaata tttttcaata gtcaggctat tatcaatact gacactacag tggttgccat
    20281 tccagcttgg cttgatatcg ctaatcacaa tatttccatc ggcatctttt atttcatttt
    20341 taatccaatg cctactgtta tcatcttcaa ttgctttttt ataaacttgc cccatacccg
    20401 ctggaacatc aaaagtaagt acagcctcat gaaaatcatt catagataaa actcctttca
    20461 aaataaaaag accactcatt gagtgatcta atatgtaaaa actacatctc aacaatgaga
    20521 tgcagttata gctctatcaa gcaacctaca ccgattccat cgattactat cgacctcgcc
    20581 ttgctcgtgt actttgagcg cccatttcca accctcagtt gccaaagtca ctggcaagga
    20641 atcgaacctt gcatggttgc cgaagcattg acctaacaca catgcttagc gtctaccctt
    20701 tccgccacag tgacaaatta atattgtgaa aataaatact aggtgtataa ttttatttat
    20761 cagcgagtgg tccgctgaaa taaatcaaaa ggtggtgaaa aatttgtact ttgttataag
    20821 agaagcaact aatggccaat attacttcgt tattaagtcc aataacaatg aagtagtagc
    20881 aactagcgaa acatacttga ctaaatattc cgctgaacaa accattaatt ctataaaaaa
    20941 cggaatcacc aaagactctc aagttatcga tatgactaaa taagacgact aacttcattt
    21001 gctagggagt tcatttcagc tgctttttct accaaagcag ttgagatact ttctagcttt
    21061 tctgatttaa cagaattaaa tactggcgtc ttgatactaa tcgatacatc ttgacgagga
    21121 tcaggactcg atgctctatt tttttgcacc actgatctgt acttttctac tgaatgatac
    21181 tcaattactt gttctaaatg ttttttgtac tctttacata cagcttcttt ttggatcaat
    21241 tctaaacgtt gttgctcaat aatatcaatc aaccgttttc gatccatacc ttgatacttt
    21301 atttgttcga cttggtcact agccttgttg agcagttctt ttagttcagt caaatctaat
    21361 ttgacagttg ctttgatttc tttatccatt cggttaccct ccaatacata aattaataga
    21421 cagcagcagt tgaaagacga caagaacgtg tttagttttt ttgtatttgc tgctgtctaa
    21481 tcaaagcaat agaaaagagc cacagatacc aattaataaa acggtcggga tatcttggct
    21541 ctttcatatt tttttgacac taatagaata tcatgctgct aacaatgttt acatagctac
    21601 aatgacctaa catttagtga acattgtaat tttagaataa agagagctgt tctttgtact
    21661 tcttgctatt gtaaaaatca atttcgtttt cttttttcaa tcggtcagct tgtctgtgtt
    21721 cgtactcatc taaaaagttc aatgtctttc tgatttctgc atgtcgctgt ctgacgtacg
    21781 atgagctgta gccaacagat tcagcaatat cttctaagct catttgatca acatatttca
    21841 tcttaacaat ttgattatca ataccagaaa agctatctat cagcaataac atttcttctt
    21901 tttgctcgat caacaattcc aattcatttt ctatcttttg gatattctct tctaaagaag
    21961 aagttctcga attcttttcg atgcggacgt ttgctaaatc accacagacc catcgattca
    22021 attcaagctt acttttatta agattccact ttaagtaaag aatctgctca tctagttctt
    22081 ggtaatcttt taaccattga aatctcacaa acgccacccc ttatgataaa atagtattgc
    22141 gctgctatct cgaaagaggt ggcttttttt attttttctt agccgttcca ttctggattg
    22201 ccttttgttc caaacgacgt tttttcttct tgattttcga ttttgttttt cccatacaaa
    22261 catcctttct ggaatatata tcgttcgatt tttactgttt aacaagagat gaccaaattt
    22321 tattgttcgc tttgaatcat ctctttgaaa aaattttagt atttttgtaa gatcccctaa
    22381 atcagctttt tttatttgat ataatatctt cacaaggtat tttacttgta aattaaatcg
    22441 aggtgataat aatggaaatt gaaaattttg aacaaaaaaa aacagatttt atcaaattta
    22501 ttaattgatg gttttgataa cgcaaactat tcacataaac ttttatttaa aagcgagcta
    22561 gatgatgaaa aagaatttga taagcaaaaa gacttaatgt gcgccttaac gtatttaaat
    22621 caggctcacg ccagcttcac aaatgcctac acctttattg ctcttaacga tgaacttctt
    22681 ggtggtcgtc aagaatttga caacatatta catcagttca ctgtatttaa tacagaattc
    22741 ttaaataatg tacgaaccaa ccacagccat caatggagtg atatagaatt taaacgttta
    22801 gtagatttct ttgaagcagc aagcggttta ttaaatggcc acgaaagaat tcaaggtctg
    22861 attaatgagg ctagaaaata gacaataatt gtttcccagc cgaccttaca cgtcggcttt
    22921 tttgcttttt ttaacatttc taactattta gccgagcaac tattattcgt tacgcgatat
    22981 tcttttttta tttatcccac ttggtaaaaa tcattggttc cggcataatc tgatcatcct
    23041 ttagcaacgc atcaggatat ttttttctta gcttcttatt attcgtatat gttttaagca
    23101 accattgact tgcttcactt ttgctttcac cggaaaaata aattttccct gtttggatgc
    23161 ctgcaagttg aatcatcatt tccacctcaa attagatttt tttctgctaa atggagcaaa
    23221 agcaatccga catcaagaat cttttgatcc attatttcag ctactttgct cggcttaact
    23281 ccttgagaaa agagcaagcg tgcttgctct atctcttcgt gagtccaaat aaatttagct
    23341 tcctcaagga taattactgg atcagttgct ctcattgttc ctctggaaaa atgatgtcgt
    23401 ccttatgttt ggaccagcta tctgcatatg gagcaaagta atgtctagct aattcgattt
    23461 gatctattaa agcaatctct gataattcat gatctgcagc aatttcactc atactatcgc
    23521 cttctgctaa acggataaga acgtctcgaa tatttacggt gatattttct ggtagttgta
    23581 atgtcgttgc tgttttaata aattcgtcaa tcgtttcttt cgatactaag attgttacat
    23641 tttcaacttc ttctacacca tcgccgacat caagtgaagt ctgctcttct tttagcatct
    23701 caattgttcc gtcagaattc actacgtatt caatatttgg ttttttcgtt tgtttgttta
    23761 ctggcactgt gtattcgatt gtttctggtt gaattactac agtcactgtt gatccaagaa
    23821 actcagttag attttcagtg ctacctctca gcgatccatt gctaacaact agtaatactt
    23881 ctgtgtttcc attagatttt gatgtcactt tcttcaattc tggtctgaaa tttacttgtt
    23941 ttgtcatttt attttccctc cattaatttc ttttttcttc gttccgtttg tcttttccgc
    24001 tgctgtcttg ctgattcaaa acaatccatt tcaatgaatt tattgatttt tgaatcgaac
    24061 ttagcaaatg tcactggaaa gccgtatcga tgagcaaaca ttttcatttt cagcatagag
    24121 atcgcatctt gataaccttt gacatcaatc acttttttca cttggccatt ctcataaatc
    24181 acaaaatcag ctcggtaata aatcggtgca atcttacgat cgttgcatag atacccttct
    24241 tgcagcagaa cattttcttg catggcacag taggtttcag ataaaggcat aaaactcata
    24301 ccgtttcgtt ttaacttcat gtaataacga gcttctgctt tagaatcaaa agtgatgccg
    24361 tctacttcgt gctttttgtt tccgtatttg cttcttgttg ccatttgcta tgctccttct
    24421 ttagtctttc tagtcgctct tttgaacgtc gtccattttc attacagatt ggacaaggtt
    24481 cacaagttga ccatccaagc gatgtcttat accaaataac cgtttcatcg ttgcatttta
    24541 ggcaagtcat tttgcacctc tcatccaagc ttgattgctt ttcggacggc aagcctcttc
    24601 ccttactggt atagcttttg gtcctgaaaa acgccgttct ggtttaataa cctctactac
    24661 ttcgccacca cagacgctgg caacaagctg ggcttcttct tttgtttcgt ggtgatccgc
    24721 atattcaaga cttgcttcac catatccacg atcattcaga taacacattt tctttactac
    24781 aaaggtcaaa ctggactcct ccttttatct gtcgttgttt tgaatacagc gatgtttcct
    24841 ttcgttccat tcatcacacg agagaacact cgctcgccgt atgcgtgttt taattgcttc
    24901 gatgataaat tagttgtgaa gatcgttgct ttgtttagac gagcttctgt gagcgatgtg
    24961 agaacatcga catcgtatgg cgttgcttgg ttgttttctt ccattcttcc tagctccgca
    25021 cctaaatcat caatcactac aaaatccgtc tttttaattt cagccatcaa cgaacctgtc
    25081 acagcttttc ttgcttctgg atcattcatt gcaaatttca attgctctaa taattctcga
    25141 tagcttataa atgaacaggc aattttataa ttagatgccc tcatcacttc tttgatcact
    25201 gcagcaccta gatgggtttt tccggctcct gttctaccag taataaccgc gtgaatcgtg
    25261 ctgccactta cgattttttt agcccattct tgagccatcg cttttatttg tgctgtttcc
    25321 tgatcaacta ctttgtatcc atcaaaatcg aaggtccata tggcatcatc tgtaatcatt
    25381 gaactgttaa gcattcgatt gatagcgtcc tttttcattg cttcatttgc tttttggata
    25441 gcttcttttt gatttttagt taccatttct cggtaaccac aaatcttatt gatacatgta
    25501 ggaccgcatc gatcagtacc atctttattt tttgttttcc agatatacaa tggatcacca
    25561 catttcgggc aattatgatc tgctgttttt aatccgccgt aaggtgcagc cattgcactt
    25621 agttttttat tcattaccaa tcctcacctg tttctggtaa ctcatcatta gaaatatttt
    25681 gttgctttct tttatttttt tggatagcca tcttatcgaa atgctttctt aagctagtcg
    25741 gtgacaaaat cacacctgac cagaaatcat tacttgtggc ccacacgatc atgtcttgaa
    25801 cttctctacc agttcgtttg tcagattcaa ttgttaaacg aatcgtattc gcccatttat
    25861 ccaaatcagg ttccttgatg ttttgatttt ttcgaatgag ttttaataaa agaatggcca
    25921 attttttatt tggatcgtcg tctgcatact ctcgtgtttt tcgagagttg cgaggattat
    25981 tatcattctt atcattctta tcattcttat cattcttgtt agttgttagt tgcttgttag
    26041 ttgcttgttg gttgcttgtg agttcctttg ttagatcgtt gtcttttgat tggtaaacct
    26101 cccagttgac aatggttata agcctatttt tgtttgttgg ttggtttgtt aaaaattcat
    26161 atttttcaaa cctctttaga gcagttctaa cattttgcaa tgagatacct ttaccacatt
    26221 tttttctaat actatcgaga gaagtaacga attgacctgg acttgctttg tacggttcgc
    26281 cttgccattc ccactctctt tcttcatgat tcgccattga aagaagggta attaaaataa
    26341 ctttttgctc tggtgtagat tcttgccaaa taggtttctc gagtaattgg cgataaagct
    26401 ttatccatcc tccagtcatt tcatcaccac cactctacaa atcgtccatg ctagtgaatc
    26461 cagtaattgg aagattacct ctacagtact cgcacaaacc gcaatggttc ggcttctctt
    26521 caccgttctt cactctgaca attctttcga ttttttcttt taacataacc agctcataat
    26581 ttatattgtc ctgatcaatc gtgatcagtc ttgcttcact tggtgtttgt ttagaaactg
    26641 ctgcaatgat cggtgtaaac gtctttccgt attgctgtaa aagaagctct tgatagattg
    26701 ccatttgaag tacgtaacca aaattgacaa tgaacgtttc tcttgatccg tagttttcgt
    26761 tccatttccg ctcatgcatg tctttggttg ttttgatatc aacaaaatac tcatcctcaa
    26821 cattcaagca atcaatcttg cctttccatg tcgttccaaa tagttcccca gtcacgatcg
    26881 tttctttctc accttgataa atgttcatga atgcttcttc ttgcttaagt cgttcaatca
    26941 tttgttcagc aatttggaaa tctttcaaca atccatacgg cttcctacta gaaaacattt
    27001 tggatttatt ttcttctttg aacgcttcat gtacttcttt tgattcaaaa taagaatgta
    27061 cgtagtttcc tacgagtaag gctttgggat ccgacgtcgg tgcccaatcg cctttcaact
    27121 ttgctaaggc tgcggcttca cattttaaaa aggacttata ttgtgatgtt gacatgtact
    27181 gccagtcagc ttcattggag taataattct catcagaaag ggtactcgtc gtcttcaaat
    27241 tctgagacat cttctacacc ttcctcgcgt ccaaagtctg gaatatctat caattcttct
    27301 tgaattggat tgatatcctc attttcgatc aaatgtgctt gctctatttc tggatgggca
    27361 ggtacatcag caactcgagt tacttcattt tggatatcat ctggaatcac aatcggatct
    27421 ggttcttttt gtattggttc agtaatcttt ttgtttgaaa agaatttttg ttcgagagta
    27481 gccacttttt ctgtttgtgg cgttacatct ttccgatcat tgtcaaattc attttctaac
    27541 gtatcttttg cagcttgaac taataaatcg ttgtcgttgc ttgtgttgat caatgtttta
    27601 gctgcccgat tgattactgt ccgtttagcc atttcttctg gaaattcctt ttgcggtcca
    27661 gaacctttca tttttgactt agcccaactt tgatcgattt gctttttcgt catgactgtt
    27721 gtggtctctt taccatttgt taacttgatg actacatagg ctgcaatgat ttcattgtct
    27781 aaattctcga attttgtttc atgctttttg acaaccatgt ttggtccatc cattgcaact
    27841 tcaaactcat caccttgtcg aacgactaca ggcgtaatat ctgcccctcc agttacacga
    27901 tcaaggaccg ccattgttcc aaaatatgaa cgcattagct gaaccttgtt tccgtattta
    27961 atgaaatagc attgtttttt tgctggagat aacccttgaa taaccatgtc taaaagtgca
    28021 ttagaaatgg aggttttcat ttcttgattg tttgcagcca tctgcaacaa gttttctcct
    28081 gtattgttag tgagttcgaa aaaagcactt tttaacgcat tttgtggact atatcctggt
    28141 ggcatttcta aaccttgttc ttgtaaccgt ccaagattac tgattacctg ctcatctaat
    28201 gaacgttgtg ttgtttgtgt taaatcattt gccattccta aattcctcct aattcaatct
    28261 ttggataatg ttgctgtacg tactctagaa ttacgtctac tggtacataa ccttcttctg
    28321 ttagatagca agtatcacct ggatataacg gattttcttg ccaatcaact ccgacaggac
    28381 tagcttcatt tggtggttgc tgtctggcac ctaaagaatc aaagtctttc atcacggcct
    28441 cctaaaattg ccaaaacaat ctgacgctgc catctcttcg tttgatattc aaaatgcctt
    28501 catcttctag cgccaaaaga aaacttggtg tcgttcctct cgatcgaaca gtgcagctag
    28561 tttgtccttg cgctgcagca gaaatgattt tttctttgat gtcctgttgt gctttgctca
    28621 tcattgcttc gaatatttca tcgcctaacc ctttaactga gatcatttat ttcctcctgt
    28681 gttataatgg tttaaaagat ttttcttttg gacacgatca tgcttgccgg cgttcgtgtc
    28741 tttttttatt tttcaaaatt actaattctt gcattttttc ttctctcctt tttgatataa
    28801 tttaggtaaa aaactggtgg tgaaaaaatg agtttcgata acacgatcac aatttcgatt
    28861 atcctagctt tagtagctct tatatcccct tggattacag cggttataaa taataagcat
    28921 gctgaatcga tgaaggataa agaaattgaa ttacaaaaac acgattcaaa aacccaaaca
    28981 atacaaacaa ctttctcaac atttctcaac aatgtgggta tttgtattgg tagtaacact
    29041 gataaaaata tatctgctgt aaaagcatca ggttatgcag tcctgccata tattcaaaat
    29101 gaagatatag aggttatgaa aattttctta agccgttttg gttacggcaa cactgatgca
    29161 gaacaaaaat ctcttgaaac ttatttgatt gacaaaatat tacctatttt gaataaatca
    29221 ttagaaaaat tgtaagcatt agacatacta gaaccgcata ggatgtatac cagtattcgc
    29281 cacgttttct catgtagtta tttactaaaa cggccacgaa ataaacacct aataaacaga
    29341 accacacttt agtcagcccc ctcggttggc ttttttgctc tgtactcagc ttcatcaagc
    29401 cccataaaaa tccaaaccat gtaaacgatc gtgccgatta atgcttgtct gcttccccaa
    29461 agtcctaaag catagacgat taggggtgcg ctgaacacta atgctctatt aaattttccc
    29521 atccgcttac ctccttagac tttatatttc gacatgaatt catcgatatc tttgatgtcg
    29581 tatttcggac ggctgttttc accgaagatg attactttca accctttttt tacccattcg
    29641 ttaatagttc ctgctgacgt tcctgtataa tgaaccgctt ctttttgagt cagatagcgt
    29701 ttaggaacgt agcctataag aaatgaatct aaatcatttt tgttgattag ttcttgtgtc
    29761 atttcgtttt ccccctctac aattcgtaca tagtaataat cgaatctata attttgtttg
    29821 cttctgcaga atctattaat ctttaatttg agctggcatg ccaattgtca gctctgttaa
    29881 tgtggcattt tttttcatat aatcttccac ttttgataca gcattttgca aattagaaat
    29941 cgtccagcct ttctcggtta caaaactaat aaccgtttca ataaattctt tttcctctgt
    30001 ttctaaaaca atcctttgcg tgtccattct attcattcct ttctttgata taattttctt
    30061 atcagtaagt ggcctactga aataatgcat aaggtggtga ataagtatga ataaaaaaat
    30121 cgctgcggat ttagttgttg catggctaaa tcacgaatca caaatatcta ctaaaggcga
    30181 atcaatttcg ccaaaagaaa ttgctcaagc ttatttagat attcaatatg ctgttatcta
    30241 tggtcagctt ccagaagacc gtaaaaacga tgactaaacg tgagctaaaa tctctgctat
    30301 ggctgcaacc atggcagagt cttctttatc taccgcgtgt tccatcgcca cttttgcttg
    30361 tttcagtatg tctaatttca aatcttcgat ttttgccgag actgtttttt ccatattctt
    30421 ctctcctttc ttttatattc gtaaacaaat ttaacaactt ttaaaaaaac tgcgttgact
    30481 aaattaacat acagtgttaa aatcagaaca tagttaaata agacatataa acattgattt
    30541 taaaagcttt cttggcggtt ggcatttatt aatcaatagt gttttttgtt gtctttttag
    30601 ttgttaaact tgtttacaag aatgatatta acaccttttg ttaaaatagt caacgaattt
    30661 taacaaataa agttaaatat tttttcttga acgaaaggaa tattgatatg ataacgtttg
    30721 agagaataaa agagttagcg aaaaaacagg gaaaatcatt aaataaagtt gaagaagacc
    30781 taggctatgg taaaaacgtc ttatatagac ttaagaactc aaacccctct acagaacgtt
    30841 tgcaagaaat tgctgactat ttcgatgtct ctgtagacta tcttctgggg cgcgaagaaa
    30901 aagaaacccc taaacatgtg gatttatcag aaaacgatac tgttttttct tttgatggaa
    30961 aagaaatatc taaggagaca atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa
    31021 atgaatagtt ggagtgatgg gttgtatgta tttgaaattg aaagaaatgt tgagcgaata
    31081 caatttgaaa gtagtttaca tggaaatgaa agagcctggc ttctattatc cgaagcctag
    31141 aatcattttt ttgaatgaaa atctatatgg cgaaactgca gaagcctttc atttatctca
    31201 tgaacttgcg catttcaccg cttctcattt tgaatattca gttttgtacg atacttctac
    31261 aacttttcac tcaaaatttg aaacagaagc agataaaata gctattctaa ttttattaaa
    31321 tatctatatt gagaacgaac tgactgatga atctcagttt aatctcgaaa aatttatgga
    31381 gtattattct attcagaata agctcagata cacttgttat gctgtctgcc aatgctattt
    31441 taagaaaaaa tattcttacg caaggcaata tgtatgaata catcaagaat tattaaaaaa
    31501 accgatttca gcaaaacatt aaaaaagccc gtgctggcac acggactcat acctcatttc
    31561 tgagatcgca aatatattat aacaagaagt gaggaatatt taaatggcaa aaaaagttat
    31621 gggtcaagac ggaaaaatgt ataaagttag taaacctttt tacaaaaaag tatggttttg
    31681 ggtattagca gtaattttaa ttataattat tggttctgct ttaaatggtg gatcagatag
    31741 taataaagca agtgataatg gtggcgaaaa agtaactaaa tcttcaacct ctgcttcatc
    31801 ttctaaagaa gaaaaaagcg ataccttcta taagattggt gacactgtaa aagttggtga
    31861 tgctgaatat acactaaata gtgtagaact aactgatgaa agaaatcaat ttgaagaaaa
    31921 ccaacctgca caagtagtaa aaattactta taccgtaaaa aatgatggcg attcagatat
    31981 ccctgtaggt acagatgtgg aagtatatgg accagatgac aaaaaatcag agacatatgc
    32041 caacgaaaat acaatgggat ctgttgctcc aggaaaacaa atggatgtaa ctgctcattt
    32101 cacgttaaac caaaagggag aaatagaaat ccacttctct cctttagtat catttgaaaa
    32161 agcagctatt tttaaagcaa ctgtataata aataaaaaac acgcccacct tccaaagcaa
    32221 gcgtgttcta agaaaaacaa acctatacag taggcttctt tatagttcct attgtatcag
    32281 agaaagagag ctgattcaat tatggcaaga ttagtcaaac gtggaaatag ttggcaatac
    32341 gaaatttcat acaaaaaaga tgacggaaaa tacacgaaga taagaaaatc aggatttaag
    32401 acaaaaggag aagcaaaaga tgccgccaac gaattagaat ataacttgaa caaaggcctt
    32461 aaaggggatc gcaaaaatct attattatca gattactttg aggattggat gcaactttat
    32521 aaagaaggaa cagtatctcc tatcacttat agaaaatacg aagatacgtt aatgaacata
    32581 aagaaatata tgccagcggt attgatttct gatttagata gagtgggata tcaacgcttt
    32641 ttaaataagt atgctaaaga ccatgtaaaa tctaccgtta t
//
LOCUS       DAXWLG010000012        30756 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXWLG010000012 DAXWLG010000000
VERSION     DAXWLG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.012"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(206..1789)
                     /gene="abc-f"
                     /locus_tag="ACGE4T_000446"
     CDS             complement(206..1789)
                     /gene="abc-f"
                     /locus_tag="ACGE4T_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFF8385760.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1894..2022)
                     /locus_tag="ACGE4T_000447"
     CDS             complement(1894..2022)
                     /locus_tag="ACGE4T_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385761.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2367..2510)
                     /locus_tag="ACGE4T_000448"
     CDS             complement(2367..2510)
                     /locus_tag="ACGE4T_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFF8385762.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2733..3212)
                     /locus_tag="ACGE4T_000449"
     CDS             complement(2733..3212)
                     /locus_tag="ACGE4T_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8385763.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3285..3569)
                     /locus_tag="ACGE4T_000450"
     CDS             complement(3285..3569)
                     /locus_tag="ACGE4T_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385764.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3647..3841)
                     /gene="ssrS"
                     /locus_tag="ACGE4T_000451"
     ncRNA           complement(3647..3841)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGE4T_000451"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3913..5199)
                     /locus_tag="ACGE4T_000452"
     CDS             complement(3913..5199)
                     /locus_tag="ACGE4T_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFF8385765.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5337..6008
                     /locus_tag="ACGE4T_000453"
     CDS             5337..6008
                     /locus_tag="ACGE4T_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFF8385766.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5974..6462
                     /locus_tag="ACGE4T_000454"
     CDS             5974..6462
                     /locus_tag="ACGE4T_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFF8385767.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6475..7422
                     /gene="prmA"
                     /locus_tag="ACGE4T_000455"
     CDS             6475..7422
                     /gene="prmA"
                     /locus_tag="ACGE4T_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFF8385768.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7423..8187
                     /locus_tag="ACGE4T_000456"
     CDS             7423..8187
                     /locus_tag="ACGE4T_000456"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFF8385769.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8420..10633
                     /locus_tag="ACGE4T_000457"
     CDS             8420..10633
                     /locus_tag="ACGE4T_000457"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFF8385770.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSNLKSIVDKIKAVPDVYSVRRTNG"
     gene            10644..11090
                     /gene="dtd"
                     /locus_tag="ACGE4T_000458"
     CDS             10644..11090
                     /gene="dtd"
                     /locus_tag="ACGE4T_000458"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFF8385771.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11261..12520
                     /locus_tag="ACGE4T_000459"
     CDS             11261..12520
                     /locus_tag="ACGE4T_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFF8385772.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12609..13556)
                     /locus_tag="ACGE4T_000460"
     CDS             complement(12609..13556)
                     /locus_tag="ACGE4T_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8385773.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13950..15242
                     /gene="hisS"
                     /locus_tag="ACGE4T_000461"
     CDS             13950..15242
                     /gene="hisS"
                     /locus_tag="ACGE4T_000461"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFF8385774.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15257..17026
                     /gene="aspS"
                     /locus_tag="ACGE4T_000462"
     CDS             15257..17026
                     /gene="aspS"
                     /locus_tag="ACGE4T_000462"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFF8385775.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17135..18010
                     /locus_tag="ACGE4T_000463"
     CDS             17135..18010
                     /locus_tag="ACGE4T_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8385776.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18276..20159
                     /locus_tag="ACGE4T_000464"
     CDS             18276..20159
                     /locus_tag="ACGE4T_000464"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8385777.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20234..20518)
                     /locus_tag="ACGE4T_000465"
     CDS             complement(20234..20518)
                     /locus_tag="ACGE4T_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8385778.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEDAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20508..21242)
                     /locus_tag="ACGE4T_000466"
     CDS             complement(20508..21242)
                     /locus_tag="ACGE4T_000466"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFF8385779.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21371..21994
                     /locus_tag="ACGE4T_000467"
     CDS             21371..21994
                     /locus_tag="ACGE4T_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFF8385780.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22186..23307
                     /locus_tag="ACGE4T_000468"
     CDS             22186..23307
                     /locus_tag="ACGE4T_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8385781.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23314..26442
                     /locus_tag="ACGE4T_000469"
     CDS             23314..26442
                     /locus_tag="ACGE4T_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8385782.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26461..29655
                     /locus_tag="ACGE4T_000470"
     CDS             26461..29655
                     /locus_tag="ACGE4T_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFF8385783.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29801..30508)
                     /locus_tag="ACGE4T_000471"
     CDS             complement(29801..30508)
                     /locus_tag="ACGE4T_000471"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8385784.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10035 a         5592 c         6528 g         8601 t
ORIGIN      
        1 agggacatta catatgtaaa aagaaaaaac atttttagat cagcctcgtg ggctgtcgac
       61 tttattcttc gctttatgtt tagctagaaa accgatccga aacattcatt gatcgaataa
      121 taaaaacttg attttccacc tgtactttgc ctgaatcgtg aaagcacagg tatgaaaatc
      181 aagttttttg ttgcgaaaaa acgtcctagt caatcagttg gccgagcggc tggcggctga
      241 tctgctcctg atcaataacg agtgcttctt ggaagtgttg ctgcaaaaag tagcggtcat
      301 ggctgactgc taggagcgtt ccttgatagt cttgcagcat ttcttcaatt tcttctcgag
      361 catagctgtc gaggtgattg gttggttcat ccaaaatcag gacgttgatt ttttctaaaa
      421 agagtttcag caacgccaaa cggatttttt caccaccgga taaatctttg atccgtttac
      481 ccacatcgct ttgataaaag ccacttttgg ctaattgccg tcgagcgact tcttcgtttc
      541 cagtcaatgt gatggcgtaa gccagcaaac ggctatcggg atcggcaaac tgcagctgct
      601 gcggcaaata accgatttgc aagctactac ctaatttgat ggtgccaccg taaggctttt
      661 cctcaccgag taataatttg agtaaggttg attttcctgc accattgtcg ccgatgatgg
      721 ctaaatgatc tccacgatag actgcaaaac tgctgtcttt gaatagtact tgatcagcgt
      781 actgctggtg caagttttga atgatgaaaa cttctttgcc agattttcca ccattagaca
      841 agctttgcag ccgatttttc gggggaggtg gcggtttgac gagtgtcagt ttcgccaggc
      901 ggcgttccag ttctttggct tttttgaaga aggactcatt gtctccttca tgtgcccact
      961 gacgaaaacg acgaatcatg actttcaacc gctggacttc tttttgctgc agttcgtaat
     1021 tttttgtcag ctcagccaag cgagcttctt tcaagactcg aaaacggctg tagtttccgg
     1081 gatactcaat caatgcacct tcttccagtt cccatgtttt tgtggtgact tgatcgagaa
     1141 attgccgatc atgagaaacg accagaaccg atttttttgt attctgtaaa tagttttcca
     1201 accagcgaat actgttaaga tctaaatgat tggtcggttc atcgagtaaa agtaaatcgg
     1261 catcggctgc taaaattttg gctaaagcga ctttaactcg ttcgcctccg ctaagttctg
     1321 ttaaccgtgc ttcctgacgg tgtggaatcc ctaagccttg caagatcgct gcaatccggt
     1381 cttctaatac gtaaccgcct agttcttcaa agcgatgctg cagctcaccg taacgggcca
     1441 gacacttctc taagtttgta tctggttctg tcatcaatgc ttcgtagcga ctcatttgct
     1501 gtttgaggtc ctgtatctca gcggcagaat ggagcaagta gtggttgacg gtcgcttcgg
     1561 aaactgtaag ttcttgttcg accgtcccga tctttaagcc ttttttgcga ctgataacac
     1621 cgctgtcgac accttctctg ccggtcaaga tttgtaaaaa ggttgatttg ccagttccgt
     1681 tgataccgac taaggcaatt ttttcaccag cttcaatttg acaggataaa tcctcaaata
     1741 atggattccc gccgattgtt tttttaatag aaattgcttg tatgatcatg atctctctcc
     1801 ttgagaccac tcaaaaaggc catggttatc catggcctgt gttatttaaa ctcagattat
     1861 catgagaaaa ataccttgag atggccattg attttacgtg aattcaactg ttttggacaa
     1921 acttgtcccg tcaacagcaa ctgtaaaacc aattgcatgg ctaaatgcga tatggtatcg
     1981 ttggtaattt tcattacttg ttattttagt catgcgcatc atagtatttt ccctccttcg
     2041 tgtagttgtc ttttccagtg tacctgaaag tgtcagaaag ttcaactagc gctgacattt
     2101 acttaatatt tatcgatgtg taattctgtc agagagcttt ttttatctga ctgagggtaa
     2161 tgtctcgtgg accacatcat tgtattctga gctaaaccag cgttcaggcg aaatcatatg
     2221 gtgtttgata aaaaactttg tctacatgac ttccttttgc catttgctta ataccgttgc
     2281 attaaacagc tcttctcttt ggaaatctca ccaacatcgc gttcaggagt atcgttgtgg
     2341 ttgcaatggc aagcggtagc ctttcatcaa ttcttgaatg atttaccgaa tgtagcactt
     2401 gccttctcgc ggtacccgaa cccgaaataa aattttagtt tggcgataaa atcggtatcc
     2461 gccatcgata cttcatattt tttcgtatca gattcatcgg aaacttccac attgacgaac
     2521 caataagctt tggcatgctt aggtcgtttt aaaccacacc agggcgacta atcgccctgg
     2581 tgttcacttt tttttatttt acttgaactc attgactgtt ttccctttat tttaaacatt
     2641 ataaagaccg atgagtgctt gccgcgtctt ttgtttttat cacggttttt tgtgcaaaaa
     2701 aacctgccga tagttggcag gtcgtctacg tgttaagctt tggtgtggtc tttagctggc
     2761 aattggtttt ctaaatcagt tcggacaaca agcacatcgc aagctgcgtt tctaataaca
     2821 tattcagaaa cagaaccaat aaataaacgt tctacagcat tcaatcctgt ggctcctaac
     2881 ataatcaaat ctacttgatt atcttctggg atttctctgg caataatttg ttttggtgag
     2941 ccatattcta cgacagaggt tacattattc aagccagctt tcttcgcatt gctttcataa
     3001 tctgccaatg tttgctttgc catttcagta gcttgttctg caagcattcc atcaaaggat
     3061 gaaacggttt ggaaagaacg tgtgtcaatg acatgtgcta atagaagttc tccgttattt
     3121 cggatagcta cattgaccgc ctttttaaaa gctaattcag cttcatcaga accatctacg
     3181 gcaaccatga tttttttgta ttgttgtaac ataagcattc actcctcaag tcaatcgtat
     3241 tgtactcttt tattgtagaa cactttaggg aaaaagagaa ttatttactg ctttggttca
     3301 gcaatttttt ttgcaaatcg gatactgaag gtggcagaaa caataattac gatcagtaaa
     3361 aagaccaagg caataaagcg ttgattgaaa aaagctgtta aaatacccat tacgcctaaa
     3421 acagcatatg cagctccgta aaattgtagg aatccttgat ttttatcatt tttgttgatc
     3481 aatacaaaaa agatatcctg tttattaaat aaataatagc taacgaataa taatactaaa
     3541 gtggcgataa tcattaaaat cctaatcatg aacaaatcct ccaaaataag caacaaatag
     3601 aaaaagcaat ttgccaagag caaattgctt tattctttaa aagatggtaa tctccgttaa
     3661 tgccgttgtt tgtcctgtag tattgatccc gcgaattcaa gcaggtgggt cccatggtcg
     3721 tagcttcgaa ctaccaaatg ataaggcatg ttaccagcag tgacacaaga cgggtcccaa
     3781 gatatcaata aaaatgttcg gtcaacacac aaagaggaca attcgaacat cacagatttc
     3841 ccacctctat agtagcatat gaatttaaaa agagcaatga gagagctcgc agaaacagaa
     3901 ccgttttctt gtctagtttt ttggaggatg ctttttccac tcttttatac ggtaatactg
     3961 ttgacccaga gcctgttcat atttccctgt atcttttggc tgataatact gagcattctt
     4021 cagtttatct ggtaaatatt gctgattgac ccaagcctga tcaaaatgat gaggatactg
     4081 ataacccacg ccacggttga gtgatttcgc acctttgtaa tggctatctc tcaaatgatc
     4141 aggaacatct ccagctttgc cctctcgaat atcagcaagt gctgcatcta atgccatgta
     4201 ggcagaattg gattttggcg acaagcaaag atcgacgacg acgtctgcta gtgggatacg
     4261 tgcctcaggc aatcccaatt tttcagcagc taatacagcg ttcactgtac gagccgcagc
     4321 agcaggatta cctaaaccta tatcttcata accgataacc atcagacgcc ggcaaatact
     4381 tgccagatca cctgcctcaa ctaaacgggc gagataatga agtgcagcat ccacatcact
     4441 tccacgaatc gatttttgaa aagccgaaat aacgtcataa tgagcatctc catttttgtc
     4501 atgggtaaga gcttttcttt ggacacattc ttcaatgatc gacagtgtaa gatgaatcct
     4561 tccttcttta tctgaaggag tcgatagtgt agccagttcc agcccattca atgcactccg
     4621 caaatcgccg ttcgttgcac gcgaaaggtg aagaagagca tcttcgtcta actggattgc
     4681 ttgttgtcca aggccgcgtt ctttatccct taaagcatgt tcgactgcca actggatgtc
     4741 ttgttctgtt aatggtttga cttcaaagat ctgtgtacgg ctgcggatcg ctggattgat
     4801 ggtaatataa ggattctcag tagtcgctcc gatcaagatg attcggccac tttctaaatg
     4861 aggaagcaga aaatcctgtt ttgttttatc taatcggtgt acttcatcca ataagaggat
     4921 cactgtacca ctcatttttg cttcttccgc tacaacttgt agatcctttt ttgaatctgt
     4981 agcggcgttt agcatgcgaa atgcataatt agtagagccg gcgatcgcac ttgcgatact
     5041 cgtttttcca gtaccaggag gtccatatag aatcatggaa gagagcatgc gagcatcgac
     5101 catgcggcgt atgatttttc cttccccgac tagatgctgc tgaccaacga cttcatcgat
     5161 cgttcgaggt ctcatgcgat aagccaatgg ctgttgcata ggattttcct ccttcttgtt
     5221 tttacgtccc ttaattatat catatttagc aagatgccta gaaagaacag ccttctgttg
     5281 ttttgttttc atagcttatc caactaatat tgtgtatgat aaagtaggtg agaaaaatga
     5341 cggataaaca aaagatagat gaattttttt ggaataaaac gacgccagaa gtggcacggg
     5401 atttattggg gatgtatttg gagcatgaca caccagaagg aagattagct ggatatattg
     5461 tagacgcgga agcataccta ggtccagaag acgaggccgc ccatagctat ggtttaagaa
     5521 gaacaccgcg tgtgcgggca atgtatgaaa aaccaggtac gatctacctt tacacgatgc
     5581 atactcatcg gattttgaac atcattactc agccagaagg gattcctcaa ggagtaatga
     5641 tacgtgcaat cgaacccgcc gcaatgattg atcaaatgag taaaaatcgt ggcggaaaaa
     5701 ctggtccaga tatcagcaac ggtccgggaa aattagtgga agcattagca attcctcaag
     5761 aactttatgg tcagtctata gcagatagtt cgttacgctt agtttttgag aaaaaaaaga
     5821 caccaaaaaa gatcaatgct ttgcctagaa tcggtatccc aaacaaaggt gtatggacag
     5881 aaaagccgtt gcgttttgtt gtgtcgggaa atccttatat ctctttacaa agaaaaaacc
     5941 aaatcgagaa agattggggt tggaggaaag aaaatgaaaa agaaggatct attaacatat
     6001 ttagatgaaa taattgaaaa acaagtgaca gactatgatg tagctttaga ttgggataca
     6061 aaaaatcata cgatcgagat cgttgttcgc ttgtttgcag aaaataaagc acaatttgag
     6121 attgatgatg cagaagggat cgtttcgcaa gaaccaatta tcgaatttga agacggcgta
     6181 cttcttttta atcctcagaa atctgttttt gatgaacgca attatctagc agtaatccct
     6241 tatgaaggaa aaaaagggtt ggcaaaatca gtagctgatt ctttagtgga atatctaaat
     6301 gaagtattag ctcaagggca aagcgatttg cttgattttt tggatgaaga cgatgacgaa
     6361 gctgtattcg agcttcactg ggataatcag caactagcag aattagtaga agtaaaacag
     6421 caaaatgaag cagatactta tttaccgtac ccaagttatt aaggagtgac agatatgaaa
     6481 tggaatgaag taaaagtaga aactgaaagt gaagcagtcg aagcagttgc taatattttg
     6541 atggaggcag gggcgagtgg tgtcgcgatt gaagattctt tagatgttga acattttcag
     6601 tcggatcaat atggtgagat tttagataaa aatgatttta catcgctgaa agaaggcgca
     6661 ctagtcatgg cttattttcc ggaaacgact ttcttaccgg aaatcttgcc ttttatcaaa
     6721 gatcgtatca cacaattacc tgagtttggt ctagctattg gaaaaaataa agtaacagtt
     6781 agtgaagtag aggaaagcaa ctgggcaaca gcctggaaga aatactatca tccggtgcgt
     6841 attacgcgaa tgctgacggt tgcgccaagc tgggaaaact accagacaga agatcctcta
     6901 gaaaaaatta tcacactaga tcctggtatg gcatttggca caggcactca tcccacgact
     6961 tccttgacat tgacagcact agaaacagtc ttgcgtggtg gtgaaacatt gctagatgtg
     7021 ggaactgggt cgggcgtgct aagtattgca gcaaaatatc ttggtgcaaa agatgtctat
     7081 gcctttgatt tagacgaagt agccgttcgt tcggcgaaag aaaatatgga tatgaatgaa
     7141 atagccaaag atgtccatgt atcagctaac gatcttttga caggaatcga gattgaaagt
     7201 gatgtaatcg ttgcgaacat tttagcagat atcatcttat tgatgatccc agatgcttgg
     7261 cgtttactga agcaaacagg cacgctgatc gtttcaggaa tcattgaaga gaagaagcaa
     7321 atggttctgg atgccatgtt agaacaaggg tttatcgtgg atcaaatttt ccagcaaaaa
     7381 gactggtatg caattatttt gaaaaaacca gaggtggaat agatgcagcg gtatttttta
     7441 aaagaatcat atgaatcaaa agactatttt gttttagaag gggaaaacta tcaccacatt
     7501 gtacgggtta tgaggatgga acccgaggat gaagtttttt tggcattcaa tgatcaaata
     7561 gccattgttg cagaaatcac ggagattact gaagaggctg tttatctgaa agaaattaaa
     7621 aaagaagaaa atcaaaagga actgccaagt tcgattacca tagcgagtgg ctatcctaaa
     7681 ggggaaaagc tggattggat cgttcagaaa tccactgaat taggagcgca agcttttctt
     7741 ggtttcccgg catcagcctc tgtcgttaaa tgggatgcaa aaaaacgtat gaaacgacaa
     7801 caacggcttg aaaagattgc tcaagaggca gcggaacaat cccatcggca gatcgtgcca
     7861 tcagtagagt tattggaaac gaaacaagat ttatttgagc ggctagatga ttatgacgtg
     7921 gtcttggttg cttatgagga atcagcaaag caaggcgaaa aatcaaatgc agccaaaatt
     7981 cttagtgaaa caaaaccaga tagtcgaatc ttagctgtct ttggaccaga aggtggatta
     8041 tcccgcgatg aagtcgatta ttttgtagaa cacaaaggga tactttgtgg attaggacca
     8101 agaatccttc gtacagaaac agcacctctt tatttattaa gtgctgctag ctatcaatgg
     8161 gaattaatga atccttcttt cagctaacgt tcttcattaa taaaagagta tcgacagaaa
     8221 gaattgaaaa aatagtcaac aatttgtata atagaaacaa ttatttatac aatggatacg
     8281 aaaaatcgtt tgaaaaagct tttaaaaagc gaacatttcc gtaaaaaaac gtataactgc
     8341 acggattttg ggacaattat caaacagata tttgtcccga ttcccgtttt accgaaaaaa
     8401 gaaaggggga tcgattcctg tgacaaaaga agaaatttta acgggacccg gtgtcataaa
     8461 gctcgttagt caatacatgg gtcccgaaca tgtagcattt gttgagaaag catgcgaata
     8521 cgcgactgct gcacatgacg gacagtttag aaaatcaggc gaaccctaca ttattcatcc
     8581 tatccaagtc gcaggtatat tagcagattt aaaaatggat ccccatacag tggctacagg
     8641 cttcttacat gatgttgttg aagatacaga aatcacttta gaagatctga gagaagaatt
     8701 tggcgatgac gttgctatgt tagtggacgg cgtaaccaaa ttagggaaaa taaaatataa
     8761 atcccacgaa gagcagctag cagaaaacca ccgaaagatg ctgctagcaa tggctcaaga
     8821 tttacgagtt atcatggtca aattagctga tagactacat aacatgcgta cgttgaagca
     8881 cctgcgagaa gataagcaaa gaaggatcgc tcaggaaact ttagaaatct atgcgcctct
     8941 tgcacatcgt ctagggatca gccggatcaa atgggaatta gaagatacag cacttcgtta
     9001 tctaaatcca aaacagtatt accgtatcgt ccacttgatg cagacgaaga gagaagaacg
     9061 tgaaaaatac gtaagtggta ctgttgaaga tattcgaata gcgacagaag agctggggat
     9121 ttttgcagaa atctatggac ggccaaaaca catttattca atctatcgta aaatgaaaga
     9181 tcagaaaaag cagttcaacg aaatttatga cttgctagct attcgagtga tcgtagattc
     9241 aatcaaggat tgttatgctg tattaggagc aatccacaca aaatggaaac caatgcccgg
     9301 cagatttaag gattatattg ccatgccaaa agctaatatg taccaatctt tgcatactac
     9361 cgtgatcggt ccggcaggaa atccggtaga aattcaaatc cgaacacagg aaatgcatga
     9421 aatcgctgaa ttcggggttg ctgcacactg ggcctataaa gaaggaaaaa atgaaaaagt
     9481 agaaccagat ggtatgacga aacaattaag ctggttccat gagatactcg aacttcaaga
     9541 cgaaagctat gatgcttctg aatttatgga aggcgtaaaa ggagatatct ttagtgataa
     9601 agtctacgtc ttcacaccaa aaggagacgt tactgagtta ccaaaaggat ccggaccatt
     9661 ggactttgca tacagtatcc atacagatat cggtaacaaa accactggtg caaaagtaaa
     9721 tggcaaaatg gtgcagcttg attacaaatt gaaaaacgga gatatcattg agatcatgac
     9781 ttctccaaat tcatttggcc caagtcgcga ctggttgaaa ttagttgcta ctagcaaagc
     9841 aagaaataag atcaaacgtt tcttcaaagc ccaagatcga gaagaaaatg tgatcaaagg
     9901 ccacgaatcc gtggtcaaat gtattacaga tctaggattt acgcctaaag atattttgac
     9961 gaagaacaaa ctgcaagaag cactcgatcg ttttaattat caaacagaag atgatctcta
    10021 tgcagctgta gggtatggag aagttagccc cttgacgatg gccaatcgtc tgactgaaaa
    10081 agaacgtaaa gaacaaaaaa tcgagcagca aaagcaagaa gcagaagaaa tcatgaatca
    10141 gccgaaaaaa gaacctgaca aaatgaaagt acgtcatgaa ggtggcgttg tcattcaagg
    10201 ggtagaaaac ttactaattc gtatcagtcg ctgctgtaat ccgattcctg gtgatgatat
    10261 cgttggttat atcactaaag gcagagggat atccattcat cgtcgagatt gtccgaatgt
    10321 tcagcctgac aaaccaaatg tagcagaacg tttgattgaa gtcgaatggg aagatacatc
    10381 gaatacacga aaagagtatg atgcagattt ggaaatttac ggctataatc gttcaggctt
    10441 attgaatgat gtacttcaaa cagtcaatgc gctaacgaaa aatctcaaca gcgttgaagc
    10501 acggacgaat aaagataaaa tggcgacgat ccatttgacg gttggtatcc agaatttatc
    10561 caacctaaag agtatcgtgg ataaaatcaa agcagtacct gatgtctaca gtgtacgccg
    10621 gacgaatgga taggaggaca gatatgagag cagttgttca acgggtaagt aaagctgctg
    10681 tgtcagtcga tcagcaagaa attggaaaaa ttggctacgg cctacttgtt ttattaggca
    10741 ttcatgaaac cgatacgcaa aaagatgtgg actatctggt aaaaaaaatt gcacaaatgc
    10801 gtatttttga agatgaacaa ggaaaaatga atattagtgt agaagatgtg ggaggacaaa
    10861 ttttaagtat ctctcaattc acactgtttg cggatacgaa aaaaggaaat cgaccaagct
    10921 ttgtggaagc cgcacgacca gaaacagcta ttcctttata cgaatcattc aatgaaggaa
    10981 tcagaagtcg cggcatcaca gtcgctaccg gacaattcgg agcagatatg tcggtttctc
    11041 tcgtcaatga tggacctgta acgatcatta ttgattctaa aaacaaataa agtaatctgg
    11101 ttttcactag taaaaactat gaaacacgtc gcttaagtta gactgataaa ctctaactta
    11161 agtggctttt ttgtctgcaa aaagcgcttc ttttgtcagc agtcggaaaa aaagttaagc
    11221 ttaaattata caagttattt tttataggag gttgctgaag atgaaagaag taccagtagt
    11281 agatatcaaa atcacagatt taaaaaaatt gtatcaaaaa ggatacaaca tgttggagga
    11341 gttacgccat gaagcggacg ctcccgtcgt caaagcaaag attttcaata aagaagctat
    11401 cacgatctac ggatcatcag cagcaaaagt attttatgat ccaaggaatt tcaaacgaaa
    11461 aggtgctatg ccaaaattag tattgaagac actttttggt caaggtggag tacagacttt
    11521 ggatggagct gctcatcacc atcggaaaaa tatttttatg gatctaatga caccggaaag
    11581 aatggaagat tatcatcgta tcttagataa gaatctgaca caagcacttg aagcacagca
    11641 tggtcaattt gaactatttg atttaagtaa gatggttttc tttacatcta tttgcgagtg
    11701 ggcaggtatc aatttatcag cgatttctaa agatgaggtc gaaaaattag cagaatatca
    11761 aatctctatg atcagcggaa cgtttacttc tccgattgat cacataaaag gtgtcgaaaa
    11821 tcgcaaaaaa tcagaaaaat gggcgcaagg cttgattgaa gaagctcgac aaaatcctgt
    11881 ggctggaaaa gaaaatgtgg cattatatgc ttttgccaat gccacagatt tagatggtca
    11941 gcttttacct ctggaagtgg cagctgttga attattgaac atcatccgtc caacggttgc
    12001 tctaactgtg tgggcggcat tgatgggaca tgcacttttc agtagaccag atttgtatca
    12061 gcaattaaaa aatgactttt cgacgttaca agatccgttt attcaagaaa tgagacgcta
    12121 ttatcctttc ttcccaatgc tacctgcgat ttcgctgaaa gaagtagaag tagatggtta
    12181 tcgtatacca gaaggcagtt gggttatttt agatttatat ggaacagacc atgatgaacg
    12241 aacagttgaa gcgccagatt cctttatgat caaacggtat gtagggaaag caaaagatat
    12301 ttcctataaa gaagaatatg agatgattgc acaaggcggg ggaaattttc gacaaatgca
    12361 tcgctgtgca ggagaatgga tcactcttca tagtctacgt gtgttttccg atcagctagt
    12421 aaataagttt gagttctctg tacctgaaca agattggacg attccattca atcagtttcc
    12481 gacttatccg aatagccgag cgctacttta caagaattag attagcataa acttatcgaa
    12541 aaaaaggaag atcaaacagt gctgatcttc cttttttcat attactataa attgataagg
    12601 tcaattgatc aaaatccacg aaatgcttca tcaatggttt gataatcttc attggataaa
    12661 tgcaaatcta aagcttggac attgtcagat acttgctctg gacgcttagc tccaggtata
    12721 actacagaca ctcgtggatt cttcatgtac caagctaaaa caagttgcgc aggagtggct
    12781 tggtatcttt tagcaatggg gcgaagtaca tcgacagcgg ttaaaatttc tctgaaacgt
    12841 tctccttgga aatctggatt tctttttcga ggatctcctt caccaaattg tttttcctca
    12901 ccaagttcat attttcctgt cagcaaaccg gaagcaagag gaaaataggg aacaaaactg
    12961 attttgtttt tttcaagata agggaataat tctttttcag cctgccgatg gatcaaactg
    13021 tatttatctt ctactacatc gacatatcca tctgcattcg cttctttcaa ttgttctaac
    13081 gtgaagtttg acacaccgac tgctcggatt tttcctgctt cttttaattc gtgcaatgta
    13141 gcaactgatt cattttttgg tgtgctttca tccggaaagt ggatataaaa aatgtcgatg
    13201 taatcagttt gcagacgttt tagtgcatct tccaccgatt gtttgagaaa ctcaggagaa
    13261 ttatcaaatg ttcgcccttt atttggtaca tgagcagctt ttgtagcgat gacgacacgt
    13321 gagcgatcat attctttttc cttcagtacc tcgccaatca gttcttcgga acggccgttt
    13381 ccgtaagcat aagcggtatc gatcaaattg atcccgctat tcaaagctgt acgaactact
    13441 tgttttcctg tctcatcttc caaaccgtca aaaagattat gtcctccaac ggcatttgct
    13501 cctaatccaa gctgttctgc gtagacttgt gaatgtccga ttcttatttc tgccatagct
    13561 gattcctcct ttttgtctag tctaaaacaa aataccaaaa agctgaacta aaacgttttt
    13621 gtgaggggaa atagtcagat atctcttgac tttgagactg gtttttagta ggttataagt
    13681 aatatcgaat gtcaatgaaa gagcgagtaa gtttttgaaa ctttacaaga gaggatcatc
    13741 gaggctgaaa atgatctaaa gtttcattag cctaaagaca ctctggagat ggacgagtga
    13801 aaagccgtcc cgcgtacgag cgttattcgt aagaggaaag agaaatcttt caaaattagg
    13861 tggtaccgtg tatgaatttc attcaccctt gttcatgatg aacaagggtg atttttttat
    13921 ttacatagct aagaaaggga gagaaaacta tgagtttcca aagaccaaaa ggaacaaatg
    13981 atattttgcc aggcgaatca gaaaaatggc agtttgtcga agaaacagca cgtttattat
    14041 tcagagatta tcagtataat gaaatgcgta caccgatttt tgaacactat gaagtgatat
    14101 cccgaagtgt aggggataca acggatatcg tttcaaaaga gatgtatgat ttttatgaca
    14161 aaggggaacg ccatgtgacg cttcgaccag aaggtacagc accaatcgtt cgttcttttg
    14221 ttgagcataa attgttcggc ccagagtatg cgaaaccgtt caaaactttt tatatgggac
    14281 caatgtttcg ctacgaacgc cctcaaaaag gacggttacg tcaattccat caaatcggag
    14341 tagaagcatt tggatcagaa aaccctgcaa ctgatgtgga gagcatggtg atggcacttg
    14401 atttcttcaa acagttgggg atcacgcaaa tccggctagt catcaattca ttaggtgata
    14461 aagaaacgcg tcaagcctat cgtcaagcat tgatcgacta tcttttacct tttgaatcag
    14521 agctgagtga agactctaaa cgccgccttc atgaaaatcc attacgcgta ttggacagta
    14581 aagacaaacg agatcaaaaa tttgtggcag aagctccatc tatcttagat tatctaagcg
    14641 agcctgctca aaaacatttt gataccgttg tttccatgct ggatgcattg gctgttcctt
    14701 atgaaatcga cagtaatatg gttcgcggac tagattatta cacacatacg atttttgaaa
    14761 tcatgagtga cgcaccaaaa atgggggcac aagcaacgat ttgcgcaggc ggacgttatg
    14821 acaatctagt ggaagaacta ggtggtcctt caacacctgg cttcggattt gcaatgggta
    14881 tcgagcgatt actcttgact atggaggcag aggaagtagt tatccctgca tttaatgagt
    14941 tggatgctta cgtggtcgca ctcggagacg aaacaaatat cgaagcattg aaagtggtcc
    15001 aagcgattcg aaactttggt ttttcagctg accgcgactt catgaaccgt aaagcaaaag
    15061 cgcaatttaa gacagcagat aaagcgaatg ccaaattagt attggtgatc ggtgaagatg
    15121 agcttgctag tcaaacgatt aatgtcaaag caatggcaag ccgaaaagaa aaaagttttc
    15181 cattagaaga gatatatgaa cattttgatg aagtctatga tgaaatgact ttattcgagt
    15241 aggaggaaaa aaataaatgg cacaaagaac gatctattgc ggattagttt ccgaacaaca
    15301 attagaacaa acagttacat taaaaggatg ggtacaaaaa agacgagacc ttgggggagt
    15361 aatctttatc gacctacgtg atcgtgaagg aatcgttcaa atcgtcttta atcctgaaat
    15421 caacaaagaa gcatgggaaa tcgcggataa atgtcgcagt gaatacgtga tcgaagtgac
    15481 tggggaagtt agaagacgcg gagaaaatgc agtaaatcct aaaatggcca ctggtgaatt
    15541 cgaagtgatt gcaagtgaaa tcgtgatttt gaatcaagca aaaaccccgc catttcaaat
    15601 tgaagatgat caagcaatca gtgacgaaat acgtttgaaa tatcgttatt tggatctgcg
    15661 tcgtccaaaa atgactaaaa acatcaaatt aagaaatgcg acaacaaaag cgattcgtca
    15721 ttatttagat gataatgact ttctcgatat cgaaacacct tacctaggga aatctacacc
    15781 agaaggcgca agagactacc tagttccttc acgtgtccat gctggacatt tctatgcgtt
    15841 gccgcaatct cctcaattgt ttaaacaatt gctgatgaat gctggattcg accgttatta
    15901 tcaaatcgtt cgttgtttcc gcgatgaaga tttacgtgga gaccgtcagc ctgaatttac
    15961 gcaagtagat attgaaacaa cgttcttaac agcagaagaa atccaaactt acacagaggg
    16021 attgattgcg aaggtaatga aagaagttcg cggtatcgaa gtcacattgc catttcctcg
    16081 tatgacctat gatgaagcaa tggctcgcta cggcagcgac aaaccggata cacgttttga
    16141 tatggaattg atcgatctaa gcgatacagt caaagaagta gaattcaaag tcttccaaat
    16201 ggctcttgaa aacggcggtg tcgtgaaagc attgaatgct aaaggcgctg cagatcgcta
    16261 ctcacgaaaa gacatggatc agttaggtca atatgtagga caattcggag ctaaggggct
    16321 tgcatggctg aaagtagaag aagacggatt gaaaggtcca attgctaaat tcatgggaga
    16381 agcaacagaa gcaatcatta aagcaacaga tgcaaaacca ggtgacttgc tgatgtttgg
    16441 cgcagataaa agtgaaatcg tcgctgctgc attaggtgct atccggacac gtttaggaaa
    16501 agaattagga ctaattgatg aatcgaaatt caatttctta tgggtaactg attggccaca
    16561 atttgaattt tcagaagaag aaggtcgata tgtctctgct caccatccat tcacgatgcc
    16621 aaaagaagaa gatatcccgt tattggctga agaccctgca aaagtctatg cagaagctta
    16681 tgatatcgtc ttgaacggtt atgaactagg cggaggttct atccgtattc atactcgtga
    16741 gcttcaagaa aaagtactag aaactctagg ctttacaaaa gaacaaatgg aagaacaatt
    16801 tggtttcttg ttaacagctt tagattacgg attccctcca catggcggta ttgcattagg
    16861 attggaccgt tttgcaatgt tattagccgg agaagataat attcgtgaag tcattgcctt
    16921 tcctaaaaac ggaaaagcag cagatgtgat gagtgatgca ccaagtatgg tttcgccttt
    16981 acaattgttc gaattgaata ttgatgtaac gaatatagaa gaatagccga taaaaaggta
    17041 aaaaggattt gtcgaatacg cttcttttga caaccttgat ttaattgaaa aacactttat
    17101 aatgaagcca taacatataa agcggggctt ttccatgaaa aaaacattcg aaaattgggc
    17161 ttatcatgat tacacgacca agctttcggt atctatcgtt tatgcgttga tcgcatcagt
    17221 cgcattgaat ttcttttatc agccaggaca tatttattca agtggtatta ctggtctagc
    17281 tcagattttg acgactttgt caaaatcact gataggattc gaagtaccgg tatcaacaac
    17341 tttgtattta ctgaatatac ctttgtttgt attggcgtgg ttaaaaatcg ggaagaagtt
    17401 tacaattttc acttttatca cagtcacttt gacctccctt tgtatccata ttttaccgga
    17461 aacagtccta tctaccgatc caatcatctg tgcgttgttt gggggggctg tgatgggact
    17521 aggtattggg ctagcgttga gaaacggcct atcatcgggc ggtctagata tcgtgagtat
    17581 cacgattcgt aaaaaaacag gaaagtcgat tggttctatt tcgatctact tcaatgcatt
    17641 gatcgttttt gttgccggaa ttctatttgg ctggcaatat atgttttaca gtacgttgtc
    17701 tattttcgtc agtggaaaag taactgatgt cgtttatact aaacaaaaga agatgcaggt
    17761 aatgatcgta acgaaacatc cagaaaaagt agtaaatgaa atccaaaact gtatgcgtag
    17821 agggatcacg atcatcaatg aagcagaagg tgcttataag catgataagc agacagtatt
    17881 gatgacgatc gttacacgat acgaactgcc tctacttaga gaagcaatga aagcttctga
    17941 tccacaagct tttgtaagta ttgcagaaaa cgtccagata cttggtcgtt tctatgagga
    18001 tggcatttag atcgagtttg acaaaaattc atgaaatact gctgtagaca gacgttcgtt
    18061 tgtctgcagt ttttttctac catagtagaa tcagcaaaga aaaactgctt ttttatttga
    18121 aattttctta aagttgaaaa aaagcatgtt tattttttta aatttatggg atgatagaaa
    18181 aaaatagtag agaaaggaca cgattctact tgctattttc acttaatacc cgtatattac
    18241 gaatgaaaaa agtttatgaa aagttggaat attgaatgga aaaacaaaaa aggttaaaaa
    18301 acagccgcta catcactggg tttgatgggc tccgttcatt agctgtggtg ggtgttatct
    18361 tctatcacct tctgccaact agtatgaaag gcggctattt gggcgttccg atttttttcg
    18421 tcgtatctgg ttatttgatc acggatctgc tgcggcagga atgggaacaa aatggaaaaa
    18481 tcaatatttg gcaattttat gtcagaagaa tgaagagact ctatccggca cttgttttta
    18541 tgcttgtgac agcttcggct tatatcactt tgtttcagcg agggctgtta aacaatcttc
    18601 gaggagttgt ggtcagcagc ttactgtatg tcaacaactg gtggcaaatc aataacggtt
    18661 tatcttattt cgatcgattt gctaatgagt caccgtttac gcatatctgg tctttagcag
    18721 tagaaggtca aaactatttg gtttggccga tcttgtttgt attgttgatg gtatttgttc
    18781 gtaaaagaaa atggatcttt tatactgtgt taggtacatc cttcctctcc gccatcttga
    18841 tgatgatctt gtatacacca ggaggcgatc caacccgtgt ttattacggt accgacactc
    18901 gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa
    18961 agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat cgctggagga atctcgcttt
    19021 tggcactgat tctttgcttc ttctttttag atgatcatta caatttcatc tattacggag
    19081 gaatgctcct agtgagtctg ttatgcgtag tgctagttgc agtgacagca caccccggag
    19141 caagtttgaa tcgatggctg acaaatccag tatttacttg gattggcaaa cgaagctatg
    19201 gcatttatct gtatcagttt ccagttatga ttttttatga agcaaaggtg actgatattg
    19261 ccgatcatgt tttgttgcat actttagtgg aaatcacatt gattctaatc atcagtgaac
    19321 tttcttatcg atatgtggag cgtccgctag cacgttttga ttatggtcgt acattccaag
    19381 tgatgaaagg atggttcagc aaaccgatca tcagtaagag caaaccatgg gttgttccag
    19441 gcacattact tgtagcaatt gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa
    19501 cagccgatca aaaacaactg caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa
    19561 ccagaaacaa ttcagaaaac accactagtt cggttgacca agcgatcctt gataagtacg
    19621 agttgtccaa ggcacaaggg aataaggcac aaaaattaga actaacggca tttggtgatt
    19681 cagttatgtt ggatgctgca acggatctac aagaacttta tccaaaagta gtggtagatg
    19741 gagatgtcgg acgacaatta tatgccagtg taccgtacat tgaaaaatta aaggaacaag
    19801 atttgttgaa agacactgtt ctgatcggac taggaacgaa tggctcattc tcggaagctc
    19861 agtttgatag tgtgatggat gcaatcggtg atcgaaaagt atactgggtc aatgtccgag
    19921 taccgacgaa gcgctggcaa aatgacgtga atgcgatgct tgaacagatg acgaaaaaat
    19981 acgacaatat gacgctgatt gattggtaca gctacagtaa tgaccatagt gactggtttt
    20041 atgatgatca agtccatcca aatcctgaag ggatgacaca ttacattcac ttagtcagcg
    20101 aagccttgct aggaggaagt tcaagtacga acagcagtga gacaaacgtg acagaatgat
    20161 agctacaaaa aacttcaggc aaaaacattt gtttttgcct gaagtttttt tatatttacc
    20221 gtgtttttaa cattcatata gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac
    20281 gttctttttg ttttcgggtg agttttttga atgcatcttc tgcttttgtt gcttcgcttc
    20341 ttgaagaaaa tacttctgca tggatcatct taacaggtct tcttttttga agacgagtat
    20401 atttagcacc agtaccattg ttatgttcct ctaatcgtct agaaagatcg gttgtatatc
    20461 caccataaaa tgtctgatct ttacagaata atacgtaaaa ataatgttca tttgccatag
    20521 agcatctcac gaatctctgc agtgtattct ccatccatat tgtaagtgat caatggtgga
    20581 gcgactttga atccgtctaa tttgccttct ttgattcctt caactaacaa aatatttgcc
    20641 tcttttccag gttttgggta aacaaattgg atccttttag gtgcaattcg atagtcttcc
    20701 atgacctgaa tgatttctaa gaaccgatca ggacgatgga ccatagtaaa gcgtccattt
    20761 gtttttagta gcttacttga aatatcgatc acttcttcta acgtcgtatg aatctcatga
    20821 cgggcgattg ccaggtgttt attagggttc gttttatttg ttggcagccc tttgaaataa
    20881 ggtggattac agacgactaa gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc
    20941 agatcgatgg tgtggacagt catttgttca tttaaatggt taagcgcgat actacgtctt
    21001 gccatgtctg ctagacgagg ttgaagttcg atagcgtcga tagccgcctg ggttcgttta
    21061 cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt caacaatctt ccctttttta
    21121 ggtacacggg aaaagtgagc aagcagcaca gcatctaaag aaaaagaaaa cactttggaa
    21181 ctttggataa tcttgatatc ttctgcgtat aattggtcga ttcgttcgtc ttcgtgaagc
    21241 atggattccc tccaaaatga aaaaaatagt cgtctgtatt atactatttt tattaaaaag
    21301 aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac tatgaccata agaagggaaa
    21361 ggaagagact atgttttatc gatttatgcg cggggtagtt cgagtactat tagccttagt
    21421 taatggaaat gcccgctatg agaataaaga agttttaccg caagatgaga attacatact
    21481 agttgctccc catcgtactt ggtgggaccc attatatctg gcggtagctg cagcacccaa
    21541 ggaattcagc tttatggcaa aagaagaact gttcaaaaat ccagttctgc gatttatatt
    21601 aactcgatcc aatgcttttc cagttaaaag agaaaatcca ggaccaagcg tcattaaaac
    21661 acctgtaaag attttgaaat cgacaaattt gagtttgatc atgtttccca gtgggacaag
    21721 acatgctaca gaacttaaag gcggaatggc attgatttcc agaatgtcta aagcgaaaat
    21781 cgtgccagct gtatatcaag gtccgttgac acttggggat ctgttcaagc gtcagagagt
    21841 gacagtgcgt ttcggcgaac caatcgactt atcagatatc aagaaaacgg ataaagaagg
    21901 tatggcggag atcgaacgac gaacacaagc agcttttgac aaattggaca aagaagtgaa
    21961 ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc
    22021 ttttgaatct actgtcctgt tcagttcaga ctttttcaat gaaaacgagg gagagacgtc
    22081 aagtctgccc tctttttgcg ttccaatatg taaaatagaa gacaggtgtc atgtaatgga
    22141 tcaaagtgtt ataataatta gaaggacatt ttttgaggtg attgaatgcg ttttttacat
    22201 acagccgatt ggcatatcgg aaaaaaacta caaggttatg acttgattga agaccagcgt
    22261 catgtactat cttctatact agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt
    22321 gccggagact tatatgatag gagtgtccca gctgttgatg ccgtagaatt atacaatgaa
    22381 ttgatggtcg attggaattt aaaggaaaag atcccgattt ttgctatatc aggaaatcac
    22441 gatagcagta cgagactttc agcgggtacg ccatggttca gttattcgaa ctattttttg
    22501 cgaacacagc tagcagaagc atttgaacca gtcgagttta aaaacgtaca atttttcttt
    22561 cttccttact ttgaaccaat tgcagcgaga ttatattttg aagatgacaa tatccgaacg
    22621 atcaagcaag cggtagccaa ggtgatagat aaaatgcagg agctatttga ttcagaaaaa
    22681 aaacaagtat tgattagcca tttttttgta atgggaagtg taaaatctga ttcggaaacg
    22741 aaaatcgaag taggcgggtt ggattcgatc cctggtgaac tacttacacc gtttgattat
    22801 gtagcactag gacatttgca taatcaagct gctttgagac aggataatgc tcgctatagc
    22861 ggttccccat tgaaattctc tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt
    22921 gacctgacaa caggcctgga gttgaatttt agggagatcg aacccctgca tgatattgaa
    22981 gaaataacag cgagtttcaa agaattgctg gatcctgcct tttatcagtc cgtcaatcgg
    23041 gagaattaca tctatatcta tctcactgat agaacgatca tcccgaatat gatgaaccag
    23101 cttcggcaaa tatatcctcg tattttgggg attgaacggg caaacggtcg agcagaacaa
    23161 acaaagcgca aacgaaaagt actagaaatg aaagatcctc aaaaattagc agaagcattt
    23221 tttagtgaaa tgacggaaga gcagctaact gcaaaccaag aaaaatggct cggagaaacg
    23281 ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa
    23341 aattttggtc cttttatcaa tgagaccgta gatttttcac gtttgacaga agcaccgctt
    23401 tttttgatca gcgggaagac aggagctggc aaaacgacga tttttgatgg gatcaccttc
    23461 gcactttttg gtgaaacttc cggaagactt cgctctggaa aagagatgcg ttctttgttt
    23521 gctacaccag acgaagaaac aagcgtgact ttttcttttg aacatcagca gatgagctat
    23581 gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa
    23641 caggcagcca aagtaacgtt gaccattttt gatgggaaag gaaaagaaat aaaacaaatc
    23701 acaaaacgta cagaagcaga tcagttgatc agagaactga tgaatctcga tgcgaaacag
    23761 ttttctcaaa tcgttttact accccaagga gaattccgta attttttggt ttcatcaagc
    23821 agtgataaag agactgtttt acgtcattta ttcggtactc aatttttcca acagttcaat
    23881 gagcgattaa aagaaaaagc aaaacaacag caacaaaaat tggatcattt agagcaagag
    23941 ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag aggaaccagc agcagggttt
    24001 gaagccgttt tagcccaatg gacaaaccat caggcaatac tcgaaaaaca aatcacggaa
    24061 gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc aactagaaaa ttcctactat
    24121 cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga tattaataaa caagcaaaag
    24181 gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac gatggattgg gtattatgaa
    24241 tttacgcaac gattagtcga accaatccag cgcctacaag aaataaaagt tgaaaaaata
    24301 gaactgtcac aaaaaagtag tgagcaaaca aagaaactag cgatgactac gcagaattat
    24361 gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac aaacaaaaat caaagaagct
    24421 gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg tcgaatcctt acgtagtaaa
    24481 aaacaagacg cagaacaatt aacaatttca ttggataaag aagaattgaa tgccgagaag
    24541 ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa ttcttcaaga aaatttactg
    24601 gatcaggaaa aactgcaaga agatcgtctt gcttttggac agttaaaata tctagatgaa
    24661 aagcagcaag aaatagaaaa acaaaaagca aaagacactg ggtcgctcgc acagataaga
    24721 gaagaactca aaaaagcaac tgaagaaata acaaaaaata gtcagctatt gaaagaacag
    24781 acagaacaag cagaacagct gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag
    24841 ctttttttgc gtccagggga gccatgtctt gtttgtggat caacggaaca ccccatacaa
    24901 caattaaaac acgaacagcc aaataaggaa gatatcttaa aagtagaaca acaactggca
    24961 gaagctgagt ctgctgtaga aagaactcag caaatcattg ccaaaagtga agcacatgaa
    25021 aataatcttc aacaacaatt atcagattta gaacaaaaaa tcatggcaac gaatatacaa
    25081 ttggctgaac aagaagaaca cttgcaacag gctttgaaaa aggcatttcc tgagataaca
    25141 gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat ctttagatag acgaaaaagc
    25201 aagatggcca atgcaagaat agagctggaa gaaataagac aacaagcaga aaagctcgaa
    25261 caacaattag ctgtctacaa acagcagcag acagaaaaaa ataatcagct ccagcaactg
    25321 caaggagaaa tcaaaagttt agaacaacaa ttgcaagata cggatagtaa ctgtttagaa
    25381 gaggagatac gacgacttga aaaacagtta gaagaagact cgaatcaact agctgttgag
    25441 cagaaagcag gagtgcaatt gttgcaagat cttcatacgc ttcggacaca gcttgatctt
    25501 caacatgaac aaatagaaaa aatagatgaa aaaatagaaa aggaacaaga aagaatagct
    25561 aaaaagttag aaagccagtc gtatttttca acaattgaag aaatctgtgc atttgcacaa
    25621 cagcaagcac aatataaaga aaaccaacgt acagttactt cttatcatga agagcaatta
    25681 gtgacagctg accggctgcg tcaattagaa aatatccagc taactgagac attcccagat
    25741 acagaagaga tcagccaaca gttagaagaa ataagacgta tgatcgaaat aaagcaagaa
    25801 aagatatacg gtttacaaga acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt
    25861 tcgatttatc acacaagcca gacgaaatta gatgagctta cgcaattaca gcagctttcg
    25921 caaacattca acggagaaaa tcctaagaag actagtttgg aacggtatgt gcttcaggtt
    25981 tatcttcaag aagtgttaca agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt
    26041 tatcaatttg agctagcgga taccattggc agttatcgag ggaaaaccgg attagaaatc
    26101 aatatctatg atgatcaggc aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag
    26161 agttttattg cagcactttc tttagcatta gctcttgccg atgtgatcca gacacaagct
    26221 ggtggtgtag caatcgatgc attgtttatt gatgaaggat ttgggtcatt agatgaagaa
    26281 gctttggaaa tggcaatgga agctttagaa acgatcgaaa gtgaaggaag aatgatcggc
    26341 atcattagtc atgtaagaga attgaaagaa cgagtattac aacaaataag aattgataca
    26401 gaaggtagcg gacaaagcaa aatacgctat caacttggat aaaaagggag attggtaact
    26461 atgaaatgga gtattccaga aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc
    26521 cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc
    26581 agtgaattgt atatggttga tttggatggt acagcaaaag agatagatta ctgccaatgt
    26641 ccctattggg aggaacataa atattgtaag catacagttg ctgtggaact ttatttacgg
    26701 tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca
    26761 acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct
    26821 caagcggtgc aacctttgat tattgaatac caagtagaaa cgattgagac taatcagtat
    26881 cacccagagc tttctatttt ggcactttac ttgcgtatcg gttcattggc atcaccgaaa
    26941 aaaacatata tcgtaaaaaa catctatgat tttttacaag cttacacaga aaaagaaact
    27001 tacactgtta ataaacagta ccagtttcgt ctagataaac gggcgtttca aaaagaagat
    27061 caaaaaatac tcacgcatct tgcaggaagt gctcagacgc atcaattatt aggtacgaac
    27121 ggcttacaag tcaaaggaaa actagataaa agatatctgc ttctcccagt agaacaagcc
    27181 cgcttcctgt tagagcagat gaatcagact acacgtttgc atctgcagat aaatgaacaa
    27241 aacgatcaag gaatacaatt ttccgatcaa aacaaacctt tatcattcaa agtggaaaaa
    27301 tttggagaag attatttgct gcaagcattg aatgattttg attttttctt tatgcattat
    27361 cagtggggag ttatccaagg gacattttat tctctgacga aatatcaaca aacggtttat
    27421 caaacttgga aacaattgat tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa
    27481 gaattaccgt ctttgtttaa agagattatc cctctgctgt cagagatcgg cgacgtagct
    27541 attgcccctg cagtagaaga ggaagtagca gatatccccg tcgaatttat tttctttttc
    27601 cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg tctatggcga agtcatctat
    27661 tcgactgacc cgaagcatga ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca
    27721 gtagaagaga aacgagtact tgatctattc agaatgtatc gctaccagaa aaatgaaact
    27781 ggttatgaac gtccattgcc aagcggggaa gaactatatg cctttttccg cacagaacta
    27841 tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa aattgcgaga gttgtattta
    27901 gatgcacata aacatcaacc gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt
    27961 cgattcgatg tgacagggat agaggagcaa gaaatcgatc atgtcctgca aagcttgctg
    28021 agaaatgatg ctttttatac attagaaaat ggtcaggttc tttcttttga ttcagaagaa
    28081 tttcaacaaa caagccaagt gttgcaacaa ttacgggaat cgatacgtac agaagaagga
    28141 acgatccatg tacctaaaaa tcaaggattg atcattcaaa atcaattgga aaaaagcaat
    28201 gctactttta gtgaatcgtt ccaaacgatg gtacaagatc tcattcatcc agaaagatac
    28261 caggcacagc ttcccaaagg attgaatgca acgatgagag attatcaaaa gcaaggtttt
    28321 cgatggttga agatgttagg tcattatcag tttggcggta ttttagccga tgaaatggga
    28381 cttgggaaaa cactacagac aattgccttt ttactttcgg aaaaagaaga acggaaaagc
    28441 ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca actggcaggc agaggtgaga
    28501 aaattcgccc cttctttaag tatccaagta atcaatggga ataaaaaaga acgtgaagaa
    28561 ttactggcta aagacacaga tatccgagtg acttcttatg ccagcttaag acaggatcta
    28621 gctaattatc agtcgcaaaa aattgattat ttgattcttg atgaggcaca aatggtcaaa
    28681 aatagcagta cgaaaactgc acaagcattg agagaattag ctgtacctca gcggtttgca
    28741 ctgagtggga caccaatcga gaataattta gaagaattgt ggtcattgtt tgcaacgatt
    28801 atgccaggat ttttcccgac taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg
    28861 caaatgattc gaccgtttat tttacggcga gataaacaaa ctgtgttgaa agacttgccg
    28921 gaaaaaacag agatgaatct atattctgca cttacagaag aacaaaaaac tgtttattta
    28981 gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg attctgaagc attcaagaaa
    29041 aaccggatcg ggatacttgc tggtctgaca agactgcgac aaatttgctg cgatcctcgt
    29101 ctgtttattg aagattacca aggcggttcc ggcaagctcg aacaagtaaa agatctactg
    29161 gtagctgcca aagagaacaa acgaagagtt ttgctgtttt ctcaatttac agggatgctg
    29221 actatcttgc aagaagagtt agccgagcta gggatctcga cattttattt aagaggcagt
    29281 acgaaaccac aagatcggtt aagtatggta gacgcattta atgctggcga aaaagatgtt
    29341 tttctgattt ccttaaaggc tgggggaact ggattgaatc tcacgggagc agatacggtc
    29401 atcttgtatg acctttggtg gaatcctgcc attgaagaac aagctgctgg acgtgcacat
    29461 cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga tctctgaagg aacgattgag
    29521 gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt
    29581 aatgaagaac agttgacgaa actgactgaa gaagacatcc gcatgatttt aagtgtagga
    29641 gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa
    29701 agaagcgatt gtttatttga tttaaaaaat agcaaggagg ctgggattcc attttatccc
    29761 agcttccttg ctatcatttt tttatttcta tttttaaagg ttatacaaag attgtcttca
    29821 ttgtctctaa cagctgatca aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa
    29881 ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt agcacttttt gcacctagaa
    29941 catcattgtc gtaattatcg ccaacgtata aggtctcttc tggaagcata tgaaattctt
    30001 tctcagctag ctgaaaaatc tctttttctg gtttttggaa acctgttgct tgagaaatga
    30061 gcatattgcg ggaaggaatc caattgttca attgaagttg cttcagtttt tttgtctgat
    30121 ggtcagtcgg cccatttgtg ataattccta atgggacatt cttttccttc aagaaatcga
    30181 gggttttttt gacctcctca tgcaagcaga tattatcaag ttcttcctcg taaatttttt
    30241 gaaataagag gccatcttct tccgtaatat gagggtagtc taaatctttt agtgattggc
    30301 tgatccgatg tgctcgcata tattctagtg tccaatctcc tgccattact tttgggaagt
    30361 tttcatcact atgatggcgg aaccggatat atagtggatg catatctgca tctgaaacaa
    30421 gcggaacgac tcttttgacc gcgtttcgaa atggctgctg ctgatcatac atcgtatcat
    30481 caacatcaaa cacaactgat ttaatcaaaa ttttacacgc ctttcggttt tcattgattt
    30541 tttcacaaat ccttgactat tgtaacagaa aaaattgtct aaaaaagctg ataaataaag
    30601 gtttttctta ctatatttct atttccttga cagaataagg tttttatatt tcagtttttt
    30661 tcactaaaaa tcgtgaaaca cgtcaataag tttgatgaac aaattaatca ttatggtaga
    30721 agtcgattga tttattttaa tgcaatctct gtggaa
//
LOCUS       DAXWLG010000013        29959 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXWLG010000013 DAXWLG010000000
VERSION     DAXWLG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.013"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            19..156
                     /locus_tag="ACGE4T_000472"
                     /pseudo
     CDS             19..156
                     /locus_tag="ACGE4T_000472"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            189..512
                     /locus_tag="ACGE4T_000473"
                     /pseudo
     CDS             189..512
                     /locus_tag="ACGE4T_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            532..1335
                     /locus_tag="ACGE4T_000474"
     CDS             532..1335
                     /locus_tag="ACGE4T_000474"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFF8385785.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1584..2648)
                     /gene="fni"
                     /locus_tag="ACGE4T_000475"
     CDS             complement(1584..2648)
                     /gene="fni"
                     /locus_tag="ACGE4T_000475"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFF8385786.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2645..3730)
                     /locus_tag="ACGE4T_000476"
     CDS             complement(2645..3730)
                     /locus_tag="ACGE4T_000476"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFF8385787.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3743..4720)
                     /gene="mvaD"
                     /locus_tag="ACGE4T_000477"
     CDS             complement(3743..4720)
                     /gene="mvaD"
                     /locus_tag="ACGE4T_000477"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFF8385788.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4713..5657)
                     /gene="mvk"
                     /locus_tag="ACGE4T_000478"
     CDS             complement(4713..5657)
                     /gene="mvk"
                     /locus_tag="ACGE4T_000478"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFF8385789.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5973..6632
                     /locus_tag="ACGE4T_000479"
     CDS             5973..6632
                     /locus_tag="ACGE4T_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8385790.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            6717..7622
                     /locus_tag="ACGE4T_000480"
     CDS             6717..7622
                     /locus_tag="ACGE4T_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8385791.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7624..8256)
                     /locus_tag="ACGE4T_000481"
     CDS             complement(7624..8256)
                     /locus_tag="ACGE4T_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8385792.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    8407..8532
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8576..9100
                     /gene="infC"
                     /locus_tag="ACGE4T_000482"
     CDS             8576..9100
                     /gene="infC"
                     /locus_tag="ACGE4T_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFF8385793.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9172..9372
                     /gene="rpmI"
                     /locus_tag="ACGE4T_000483"
     CDS             9172..9372
                     /gene="rpmI"
                     /locus_tag="ACGE4T_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFF8385794.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9425..9784
                     /gene="rplT"
                     /locus_tag="ACGE4T_000484"
     CDS             9425..9784
                     /gene="rplT"
                     /locus_tag="ACGE4T_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFF8385795.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10036..10501
                     /locus_tag="ACGE4T_000485"
                     /pseudo
     CDS             10036..10501
                     /locus_tag="ACGE4T_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(10668..11855)
                     /locus_tag="ACGE4T_000486"
     CDS             complement(10668..11855)
                     /locus_tag="ACGE4T_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFF8385796.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            11961..12848
                     /locus_tag="ACGE4T_000487"
                     /pseudo
     CDS             11961..12848
                     /locus_tag="ACGE4T_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            12896..15025
                     /locus_tag="ACGE4T_000488"
     CDS             12896..15025
                     /locus_tag="ACGE4T_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFF8385797.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            15000..15689
                     /locus_tag="ACGE4T_000489"
     CDS             15000..15689
                     /locus_tag="ACGE4T_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385798.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(16097..16783)
                     /locus_tag="ACGE4T_000490"
     CDS             complement(16097..16783)
                     /locus_tag="ACGE4T_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8385799.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(16919..17086)
                     /locus_tag="ACGE4T_000491"
     CDS             complement(16919..17086)
                     /locus_tag="ACGE4T_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385800.1"
                     /translation="MYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKRIGG
                     RVKPIQEVRPD"
     gene            complement(17163..17603)
                     /locus_tag="ACGE4T_000492"
     CDS             complement(17163..17603)
                     /locus_tag="ACGE4T_000492"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFF8385801.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(17607..18452)
                     /locus_tag="ACGE4T_000493"
     CDS             complement(17607..18452)
                     /locus_tag="ACGE4T_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFF8385802.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            18601..20019
                     /gene="pepV"
                     /locus_tag="ACGE4T_000494"
     CDS             18601..20019
                     /gene="pepV"
                     /locus_tag="ACGE4T_000494"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFF8385803.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(20095..21297)
                     /locus_tag="ACGE4T_000495"
     CDS             complement(20095..21297)
                     /locus_tag="ACGE4T_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385804.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(21284..23071)
                     /locus_tag="ACGE4T_000496"
     CDS             complement(21284..23071)
                     /locus_tag="ACGE4T_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFF8385805.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(23084..24037)
                     /locus_tag="ACGE4T_000497"
     CDS             complement(23084..24037)
                     /locus_tag="ACGE4T_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8385806.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(24222..24572)
                     /locus_tag="ACGE4T_000498"
     CDS             complement(24222..24572)
                     /locus_tag="ACGE4T_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8385807.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            24772..25851
                     /gene="pepA"
                     /locus_tag="ACGE4T_000499"
     CDS             24772..25851
                     /gene="pepA"
                     /locus_tag="ACGE4T_000499"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFF8385808.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            25994..26314
                     /locus_tag="ACGE4T_000500"
     CDS             25994..26314
                     /locus_tag="ACGE4T_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8385809.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            26336..26800
                     /locus_tag="ACGE4T_000501"
     CDS             26336..26800
                     /locus_tag="ACGE4T_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8385810.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            26996..27601
                     /gene="ytpR"
                     /locus_tag="ACGE4T_000502"
     CDS             26996..27601
                     /gene="ytpR"
                     /locus_tag="ACGE4T_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFF8385811.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(27640..28929)
                     /locus_tag="ACGE4T_000503"
     CDS             complement(27640..28929)
                     /locus_tag="ACGE4T_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFF8385812.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            29357..29836
                     /gene="rlmH"
                     /locus_tag="ACGE4T_000504"
     CDS             29357..29836
                     /gene="rlmH"
                     /locus_tag="ACGE4T_000504"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFF8385813.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         9404 a         5671 c         6051 g         8833 t
ORIGIN      
        1 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
       61 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
      121 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
      181 gcttcagcga tagctgtcat ttataaatgg gttatacgat tttagaacca ggaagtgctt
      241 ggaatacgat gccttgtcat acacatgaac gtcgtatgga agcttatgtc tattttgatt
      301 atgccaatga agatacacgt gtgttccata tgatgggcaa accagatgaa acaaaacatt
      361 tagtcgtaga caatgaacaa gccattattt cgccaagctg gtcgatccat tcaggtgtag
      421 gtacaagcaa ttactcattc atctgggcaa tgtgcggaga aaatatcacc tatacagaca
      481 tggatatggt gcaaatggat caattaaaat aagcataagg gagagaaaat gatggaattt
      541 agtatggata tgtttcgttt agatggaaaa gtagcattag tcaccggtgc tgtctatggt
      601 attggatttg agattgcacg ctctttagct acagcaggag caaaaatcgt atttaataac
      661 ttgaatcaag aatcagttga tgaaggtata gcgcattata aagaagcagg catcgatgca
      721 aaaggttatg tctgcgatgt gaccgatgaa gaagcggttc aagcgatggt tcaacagatc
      781 aaagaagatg taggttcagt tgatattcta gtcaacaatg cagggatcat caaacggaca
      841 ccgatgatcg aaatggatgc ggcagacttc cgccaagtaa tcgacgtgga tttaaatgcc
      901 ccattcatcg tttcaaaagc agttattcgg gatatgatcg aaaaaggcgg cggtaaaatc
      961 atcaatatct gctccatgat gagtgaatta ggacgcgaaa cggtcagtgc ttatgctgcg
     1021 gcaaaaggcg gattaaaaat gctgacgaaa aacatcgctt ctgaatacgg tcaatacaat
     1081 atccaatgta atggaattgg accaggttat attgctacac cgcaaactgc tccattacgc
     1141 gaaacacaag aaaacggtga acgtcatcca ttcgatcaat ttatcgttgg acgtacgcca
     1201 gcagcacgct ggggcgagcc aatcgatttg gctggaccat ctgtcttctt ggcttctagc
     1261 gcatctgatt tcgttaacgg ccatgtcttg tatgtggacg gaggcatact tgcctatatc
     1321 ggaaaacaac cttaaaagag ctttagtaca ccgtttagtg gagaggttgt gaaatcagcg
     1381 gcttgaacga ataccgaagg acagctgatt aaacatcatt tattcgaaaa aagaggctgg
     1441 gacagaagcg tttaactcca agaaataaga aggaattcac gaaaattgct tttcaaattt
     1501 ttgtgaattt cagcttattt ccgaaggagt tgcttttgct tccgccgttt atacgtgttt
     1561 agagcgtggg ataaaagtgt tctttacttt tgtcccacgc tcttttctgt gcgtcttcca
     1621 tacttcacta agtcgatccc tcttgcttcg caccaatcct tcactggtcc ggaaaagatc
     1681 agtgattgtt ggtgtagggc cgcggtattc gttgcgccga ccatagtata aagcagacga
     1741 agctcttctt gccattgctt catcagtata atagtttctt ctacgccgtg attcatcaga
     1801 tgcgtcagta cggttcctga agtacccact gcatttgcgc cgagacaaag ggctttaaat
     1861 atgtcatagg cattgcggat acctccggag gctaggattt ccatagaagt gtctgcttca
     1921 tttgcttcaa gcaatgaaga gactgtcgat tgcccccagt cagctaaata actcagttcc
     1981 cgcttggagc gacgggcgtt ttcgatctgg gtgaagcttg ttccgcttcg accgctgata
     2041 tcaactgtat gaactcctaa agcagctaga tcattgagcg tttctcttgt catgccaaaa
     2101 ccgacttctt ttacgatgag ggggacgtca atggctgttt gagtctcttg aataagtact
     2161 ttccaattgg taaaatcacg gtctccctca ggcataacca actcttgagg tgcattcaaa
     2221 tgaatctgta aggcatctgc gtgaaacaat cggatagctt cttgggctct ttcgacagaa
     2281 gtccctgcac cgatgttggc aatgattttc ccgtgaggat attcttgtcg cataatcgta
     2341 taagtatcgg ctaaagaagg gtctttcaag gcagcgctga ctgaaccagt ggcaatcatc
     2401 aaatctgctt ctcttgcgac gattgcaaga tcttggttga ttttttttgt tttttcgctt
     2461 ccgccagtca tcgcattgat ataaaatggg ctgcttagtg taaacccaac tgcctgtgta
     2521 cttagatcga catcagaaac tgctatttgc ggcaaagggt tatggatgat ccgtacgaag
     2581 tcaaattcgt ttttttgttt atcatgaaag gctttagcta gtgaaacatg ttcgtctttt
     2641 cgattcatcg gttttccttt ctttgatcgc tatagacatg taacggcagt ggagtgattt
     2701 ctgctttttc ccatgcactc attaaaggaa ggatgccaga tttctggtca acaattacga
     2761 ttccgcaatc gccaccacct gcaccagaag attttgcggc tccttcatac tgttcagcta
     2821 aattacacaa tttgttcaaa gcaggcgttt cgatgacgac cccagtgatt gcagataaat
     2881 catgcagtaa ttgtcggttt tttcgaatca tctgttggat caacgttaca ttattttctt
     2941 taaacccttt gatcatttcg ttgacacatt ctgtactgtt ttttaagaaa agctgataag
     3001 ccaccatttt gtcttctctc gaacggtgaa cttgatcgac caaatcagaa gtagaggcag
     3061 ggctacccgt ccaaccaatc aataaacgta aatcttcagg agcaatcaat ggctcaatgg
     3121 atagacctgg ccaatccagg gcaagtaact cactgataga atgctgagtt tcttgttctt
     3181 ggagccaagg atggtcgaag gttgagaaag cgatccagcc accataacag ctagcagcga
     3241 tgtcgccgca ggaaccatta tcttgaactg ctaaattggc tagtgctgca atcttgaaaa
     3301 tctccaattg agataaattc aacgcgtaaa aaacattcaa ggctttgacc gttgcaacgg
     3361 tcacggcacc gctagaaccc aatccatatt tccggccatt tgaactgtct aactcacttg
     3421 ttactttcag atcataaaag gataaaagaa tgtttttttc ttgtgcatac ttttcagtca
     3481 aacgaatagc agcaaggata taatgaaaag gattttcccg aatatctaaa actaattctc
     3541 cgttgcgtct tgtccaacgt acaggcatcc cactatattg tgcagattgg atacttccga
     3601 cttttcgtgc ggattctaca gtgactgtta cgaattgatc gactgcagcg ataactgctg
     3661 gatgtcctgt ttcaacaact gcatattctc cggcaatata gagtttgcct ggtgcagata
     3721 cttcaatcat aaaaatctcc ctttattcaa taatcgcaat tcctggacca gcatgggcag
     3781 taattacttg ttctttgcta aataatttgg taaatgtttc ttgtacctca gataaatggc
     3841 tgttttccac taaaactttg acattagggc cagcatccat cgtaaagtag catggaatgc
     3901 ctttttttct caattgccgc accgcatcca tcgcttttaa gctgtcagga gaccagtaag
     3961 tgaatggagg ctgggcagct aaagtcgttc catgcatttt tagtccgttt ctttccatcg
     4021 tttctccgag aagttggaaa tcttttgttt tgattgcttg tttcaattga tatagatccc
     4081 cttcaacgga atctaaccag ccttgataaa aactggatgt ttcgactgtt cgacgcatcc
     4141 catttctgct ggacacttct tttttctggt cgttgatcaa aacgaaaacc attgctaaat
     4201 cgtcttcgaa agaatcggaa gggactggct tagcgtaaga acttaagtcg tcatggcctt
     4261 tttcccattc aacgaaacca ccgaaaatac ttcggcaagc tgaaccagac ccgcgtcgag
     4321 caaatcttga aagagagaga tcgtctaatc caagcttaag agcagtattg caagctcctg
     4381 ctaaagcagc tagcccgctg gcagaggagg ctaatccagc tgcggtagga acgaaattct
     4441 gactgattac tgaagctttt agtgagaggc cggcttcttt acgaaaaagg tcaagaaact
     4501 gactgacttt ttttgttgct ttttcgtctt gtaaggtgcc atctagataa aattcatcta
     4561 ccatatagct atcagaaaag atgacttctg tctctgtata aaatgcatcc aatgttaacg
     4621 aaaggctgtt gttcattgga aggatgagtt cttcattttt cttaccccaa tatttgatta
     4681 gagcaatgtt ggtatatgcg cgtgctttgc ctttaaacat aggtatgtac tcctaatcct
     4741 tgtatccaag tttcagcagc acccgcatct tcaagtgctt gactgatttc ttgggctttt
     4801 gtttttgttt gtgctaatgc aatcatacag ccaccgcgtc caccgccagt taatttggca
     4861 cccaatgccc cggtatcttt tgctgtttgg atcaatagat taagaaaatc attgctgatt
     4921 gtcagctttt ccagaagtga ctgtgattca tccattgtct gtgccaacgt ttctggtgaa
     4981 ttctcaatga ttgcttgttt tgcttgcttc gtcaagtatc ctaatttttg gatcatttgt
     5041 ccagtttcat gaggctgggt ttcaaagagg tgagcgacat ctttgacggc ttctcttgtt
     5101 tgtcctttga ttcctgtatc agcgacaatc aaaaaagcat cgatgttcaa agaaaagtag
     5161 tcaaaaggat gcccttttgt aaaataaatc ggctgcaagc tgctagtagc ggctgcatcg
     5221 attccactag gattaccgtg ggcgattttt tccgaaagct ggacattttc taatagtatt
     5281 tcacgagaca aaggaaatgc taagtaatca taaaaagcac gagtgactgc tgtggcgact
     5341 gcagcgcttg atcccattcc acgttcagca ggaatcgtgc tttcaatggt cagttgcaag
     5401 tttttttgga tgtcatgctg tttttttaag tgaatgaata attttttgat attttttaat
     5461 gcatgaggag cttcggctaa atttcctgaa tagtaggaag aaaccagttg atcgtccatt
     5521 gcatccagct cttcaaggaa tgcggtgact tttgttgcat agaaaggaaa ggcaatcgct
     5581 ggttctccat aaacaaccgc gtgctcgccc atcaatatga tctttccgct tgattctcct
     5641 tggccatagt ttgccataat ctaactcctt ctcacgtaaa agtgattttt aagcaaaaaa
     5701 taaagcgccc aaaacgagtt gcgctgcttc acgaattttt ttgtccacta agggtctcaa
     5761 aaaacattcc tcctttattt tactaaaacc atctcagaag taaaactaag aatacgtttt
     5821 ctaaagaaaa aacacttttt tcttaagaat tttttgactt tttttcttct ttgactagaa
     5881 aaaatacaat tgacactact agatattggg ggtatattta aaaaaggttg gagttaaaca
     5941 caatatttag tgatgaagtg agctggaggt aaatgagcgt gatcgtttta gcaggtacaa
     6001 ttggtgccgg aaaatcaagt ttaacagaaa tgatggctga acactttgat agtcaggcat
     6061 tttatgaatc gattgatgat aatgaagtct tgcccttatt ttatgcaaac cctgaacagt
     6121 atgctttttt gctgcaaatt tattttttga ataaacgttt tgcaagcatc aaacaagcaa
     6181 tgaaagatga caataatgta ttggaccgtt cgatttatga agattcactg cttttccatc
     6241 taaatgcaga tctaggaaga gcaacagaaa cagaagtttg tgtgtatgat gaactgctgg
     6301 aaaacatgat ggaagaactt ccctatgctg cccataaaaa acatccggat ttattagttc
     6361 atattcgtgt atcttttgat acgatgttag agcgtatcga aaaacgtggt cgttcgtatg
     6421 aacaattgtc ctttgaccca agtctatacg attattacaa agaattgaac agacgttatg
     6481 atcaatggta tgaagaatac aaagaaagtc caaaaatcca aatcgatgga gaccgctata
     6541 attttgtgga agatcctcaa gcaaaagaag aagtcttgaa aatgatcgaa gaaaaattag
     6601 cagagattcg ccaaaccaaa gtatccattt gatttacagt tgctcaaata aagggtaaac
     6661 ctttatttga gcgacttttt tctttatact ggaaacaaat gtcaggagga tagagtatgc
     6721 gggaaaaaca ctattttaat caagaccgta atttttggat caatgtacag caaaacgatg
     6781 tgctagctgt taaaaactta aaagaagaat atggcattag tgacgagatg ctgacttatt
     6841 ctctagataa aaatgaacgg gcgcgcgtag agtacgattc ttttgatgaa gcgttactgc
     6901 ttgtatcaaa tgttccgcac cagcaaaaag tcgataatca ttatgaaact agtcctatcg
     6961 cttttatttt aaaagaagac ggtttgttta catttaccac gccgaatacc gagtacgtga
     7021 ttcgactaat tcgatcgctt ttggaacgga tgccagacat gagtgtatac agtttattgt
     7081 ttcgtacatt gtttttgatt tctgattcct tttttccttt aatcgaggaa gtgaatagtg
     7141 aacgtcagcg gcttaatctg aaattaagag aaaaaacaac gaacaagaat ctgctgcaac
     7201 tttcagattt agagatcggt ttggtttact tagttacagg aacaaaacaa aatgtagtac
     7261 ttttagaaca aatcaaggca ctggctattt atcgcaagtt gtcagaaaag gaaaaagaag
     7321 agttggacga tgcactgatc gaagcacgac aagcagttga aatgacgaac ttagcttcgc
     7381 agattttaga ccagctctct ggcacttata ataatttgct gaataacaac ttgaatgaca
     7441 cgatgaaatt tttgacggtc tggtcgctga tcttgaccgt tccaactatt gtgacaggat
     7501 tcttcggaat gaatctccag ttacccttta cccattccgt tttcggatgg gaaatcgcct
     7561 tgatcatcag tttggtctta tctatttgga tgcttattgc actgtggcgg cggattcgtt
     7621 gatctagtct ttgatctgca aaccaagggc ttgggcgatc acaagtgctt gctcttgatt
     7681 gacgattagt cccgccaaag aatcttgaga taaagcgatt gttgtgaaga aattagtcgt
     7741 aaagtctaat cccttcagag aagttcgaaa ccagttcgaa ccggttaact gattgtcaga
     7801 caaaaaaagg tttttccaag tgacctcata aaattcgctg tcttctaatt gacaatgatc
     7861 gaaggagaca gctttcaagt ttgtactact aaaggaagcc atcgaagcaa tacaatcagt
     7921 gaaagtacag tctcgaagat aactttcggc aaaattcgta cctgttaatt tacattgatg
     7981 aaacacgact tggtggaaac tgccaccaat ccactccaga ttggaaaaat cacatttttc
     8041 aaaaatgaca ttactgcatt caaaatgacg taaacctgtt ttttgcatcg taagtttttg
     8101 gatatgactc tctcttaata ccatattttt ggcatcgaga tagctttggt tacctccgtt
     8161 gatcagaaga ccagagaaga tctcctcatc ttctaaataa aactctccct ttgtaagaag
     8221 aggaagagaa ggtggaatag gttcgatttt tttcatgtgg gttcacctcg tttttcttta
     8281 gtatagcaaa gaagcgggaa tcactgctag aaagcctgct aatctaatca ttttcagaaa
     8341 aggaagacaa actgtatgaa aaacattgac aaaaagatta gtttttgcta tactataatg
     8401 gttgagaaat aaaagcagaa gcacccgctt ctcgccttag ttgataatgt cactgggcta
     8461 gatagttttc actggttcta ttaggtgaag tacgcgcggg ttctgtaaga gaactcgttt
     8521 ttttattgcc gtttattcgg taagcttttc tttctctcat aatatttgga ggtgaatgac
     8581 catagcaaag gatatgatgg ttaacgacgg cattcgtgca cgagagttac gattgatcgg
     8641 ttcagatggt gagcaattag gagttaagac aaaagcagaa gcattgaata ttgcagaaca
     8701 agcaaacttg gatcttgtgt tagttgcccc tggtgcgaaa ccaccagttg cgcgaatcat
     8761 ggactacggt aaataccgtt tcgaacaaca aaagaaagac cgcgaagcgc gtaaaaaaca
     8821 aaaagtgatc aatgtaaaag aagttcgctt aagtcctacg atcgacttga atgacttcaa
     8881 tacaaaactt cgtaatgcac gtaagttctt ggaaaagggc gataaagtga aagcttctat
     8941 ccgtttcaaa ggccgtgcca ttacccataa agaaattggt caaaaagtct tgaatcgctt
     9001 agctgaagaa actgcggata ttgcaacagt ggaacaaaaa gcgaaaatgg acggacgcag
     9061 catgttcttg acgctggcac cgaaaaacga cagtaagtaa tttgaatgac taactggctg
     9121 ttggacagcc gatagtcgga taacgaaaga ttttgaggag gaaatatagt catgccaaaa
     9181 caaaaaacac accgcggatc agcaaaacgt ttcaaacgta ctggtaaagg cggattgaaa
     9241 cgcttccgcg cgtttacaag tcaccgtttc cacggcaaaa caaaaaaaca acgccgtcaa
     9301 ttgcgtaaag caagcatggt ttctagtggc gacttcaaac gtattcgcca acaactagca
     9361 aaaatgaaat aagaagtaca aataacagaa tctgaagatt tatttttatt aggaggaatt
     9421 atacatggca cgtgttaaag gtggaacagt aactcgtaaa cgtcgtaaaa gagtgctaaa
     9481 attagcaaaa ggttactacg gttcaaaaca cactttattt aaaacagcaa aagaacaagt
     9541 aatgaattca tacaactacg catatcgcga tcgtcgtcaa aagaaacgcg acttccgtaa
     9601 attgtggatt gctcgtatca acgcagcagc tcgtatgaat ggcttgagct actcaaaatt
     9661 aatgcacggt ttgaaagttg ctgaaatcga catcaaccgt aaaatgttag ctgacttggc
     9721 tgttaacgat gcagcagcat ttactgcatt ggctgaccaa gcaaaagaag ctttggctaa
     9781 ataagctaaa caattataaa gaggaaagaa gataacgaaa agttatgttc tttcctcttt
     9841 ttttatttac aatcctccat caaatgaaac aggacaataa cgtataagtc taaaataaca
     9901 taattaaggt tttttaattg aaaactgaac atttatggtc atcctttaat gaaagcgcta
     9961 actacataag ttagaatgag tttgactgat atattagttt gcttatcaga aaaacaaaat
    10021 ggacggagga ataacatgag tgatattatt attggtattt tgttagttat ctcattcttt
    10081 ttcatggtct ggtactgtgt aaaaggctac aacttgatgg tcggcttttg tcatcatggc
    10141 tactgtatgg atgggattag cgctagtcgg aaacacgttt tcaccaaact cggcgatgga
    10201 agggcaagga gtaattgatg tgttgacgca tatctatacg acggggccag ctgagtacgc
    10261 caaatcgatc ttggtaaatg tcttctttgg tgcctttttc gggcgtgttc tagtagatag
    10321 cggaatcgca gctaccctga ttcgaaaggt agttgaactt ggaggggaca agccaagaat
    10381 cacgatgagc ttactatgta ttgtaaccgc tgttatattc atgtcgatga caggaatcgg
    10441 accagtgatt tctattgcag ttattgtgtt gccgatcttg atgtcattag gtatttcagt
    10501 caagtccaca atcttgtgta aaagttcaac ctcatcaaaa gcacaatgtt ttgcgttgtg
    10561 aacctaactg ccatgccttt ctttctgagg agagaggtca ggagcagtag cgcagcgact
    10621 cttgatgtct ctcctcagaa agaagagact cgcacaggtt catggtgcta aatgttattc
    10681 gaattaaatt ctaaatattt tcgtggagaa gccagccact cagtgtcttt ttccatcaaa
    10741 agtgttccaa ttatccgagt gatggaattg atatttggaa aaatccgaat cactcgttct
    10801 ctccggcgaa tctcttcgtt caaccgttcc aacacattgg ttgtacgaat ccttcggcgg
    10861 atattttcag gaaaagacaa aatttgaata gcatcttcaa agccgttttc taaaatgtcg
    10921 caagcagcac ttaattttga atcacaacca tatttttcca agaagtgctc ttttctttca
    10981 cgtgtcaatt caagttcaga ggctttaaag atactcttca actcgtcttt tacatcagaa
    11041 gaaactttct ttggcagctt atcaaaaata tttctcaaaa agtgggcttg gcatctttgc
    11101 cagctcgcac ctaaaaagtt cttgcgaaca gccttaacta accctttgtg tgcatcagaa
    11161 atgaccagtt ttacaccgaa caaacctcgt tgaacgaacg attgaaaaac ggtatcccaa
    11221 ttatcttccg attcgccgtc ttgaacatca aaaccaagaa tcgtacgtcg tccatcagaa
    11281 tcaatgccga taccaatata tacacctttt gagacaatac gatgattttc ccttactttc
    11341 atatacaggg catcacacat gaaaaatgga tattccgagc cttctaggct ccggtttctc
    11401 cactcaaaaa caaatggatc caactgtttc atcagatttg aaacgaatga tttagaaaca
    11461 gtcgcaccat ccgttagcag ttcaactgtt ttagttactt tgcgagtgga gacaccagaa
    11521 atgaccattt caatcatggc agcgatcaaa gctttttcgc ttcgctgata tctttcaaag
    11581 atagtaggac taaacttacc gtctctggtt cgtggaacat gaagttctaa cgtaccaatt
    11641 cgagttgtaa acgaacgatc gtagtaacca tttctttgtg attgccgttc ttctgaacgt
    11701 tcataggcat ttgcctgaat atattgattt cgttgttctt ccatcagctg attaaacatg
    11761 atagtcataa gtgtttttgc ggtatcattc gcaccgcttt cgtttataat tgcttgaatt
    11821 tcttcgcttt caaaagtaaa atgtacttga gtcattgata tcagtcctat tcgagtatgt
    11881 tttctgcaaa aaacattgta ctcctaactg agatcaatgg cttttttgat gcttcacttt
    11941 tacacaatta tatggacgta atcggtattt cagtaccggt tgcactgttc tcatttatgg
    12001 gctcgattat ggctggaatc tttgctaata tcgtcaattt caaacaatat caaacgattt
    12061 atgctggttt caatccagcg gctgaaagct atacttataa cgactatttc caaattggta
    12121 tgattgggat gattgtaagc ttagttgtgg tactaacggt cgctaatata tcaatgaaca
    12181 aaaagaaacg ctatgcaatg gcagcaaacg taccagctga aggtggcgac gcaccaatga
    12241 tctcatggtt agccgtatta ttaccagttt taggggtcgt tcttttagat cttcaaatca
    12301 tcttaggttt tattctagca ggtatctggg cattgctgtt tactggaaaa ttgcgcggag
    12361 gttataaaga aatctgtcgt cagtttgcga aattatttac agatggcgca gtcgatgttg
    12421 caccaatggt tggtttcttg atgaccttag cgatgttcaa taattcagct gcgtatgctt
    12481 ctccttattt ttcagctatt tttggagatt ggataccgca atcaccatta gtcttggcaa
    12541 tcgtatttgc tatattgaca ccactaggat ttttccgcgg tccaatgaat ctagtcggtt
    12601 caggctcagc tattttagct gtcgtgttag ctgttaatcc aacgatgtcg ccagcctttt
    12661 tgttcccgtt atttgcaatt acaacgattg ctcctcagca tttagatatt acacaatctt
    12721 gggttgcttg gggattaggt tatacaaaag taacttctcg agaatatatg aagaaatcca
    12781 tcccaactgg ttggatcatc ggtgcgatct tatgcctgat cacgttctta ttgtacggga
    12841 acgcctagta aacaaaacgt cgataaaaac gattagataa atggaggaaa agaaaatgaa
    12901 acgttttgtg cgaatgggaa ttgatgttgg cggtacacat acgaaagcag tcgcaatcga
    12961 taatgcgacg cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg
    13021 tggagtagcc gcaggtgtcg ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag
    13081 tccagaagat gttgtgtttg ttgcacattc taccacacaa gcgactaatg cactgattga
    13141 aggagatgta gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt
    13201 agcaaaacga caaactagat tgaacgatat cgatttaggg aataagaaaa aaatagagat
    13261 tgtcaatgca ttcttacctg tcaaaaactt aaatgtagac cgtgtttcag aaacgatttc
    13321 ttctttagaa agagaaagag cggaggtctt ggtttcttct atggcatttg gagtagataa
    13381 tggggaacca gaacgagttg tttatgaagc agcatctgtg aaagcgatcc ctacaacgat
    13441 ggcttctgac atcacaaaat tatatggatt gacaagaaga actcggacag cagcaatcaa
    13501 cgcatctatt ttaccaaaaa tgctagatac ggcaacctcg acagaagact ccgtacgaga
    13561 agcaggggtc aatgtttctt tgatgattat gcgcggagac ggcggagtaa tggaaatcaa
    13621 tgaaatgaaa aaacgacctg ttttaaccat gctttccggt cctgctgcat cagtcatggg
    13681 ctctttgatg tacttgagag cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac
    13741 caatatcggg gtcattaaaa atggacgtcc agcaatcgat tattcaattg taggtggcca
    13801 cccaacatat atctcttcat tagatgttcg ggttctaggt gtggcaggcg gttcgatggt
    13861 acgggctaat cagtcaggaa tcatcgatgt cggtccacgt tctgcacata ttgccggtct
    13921 agattatgca gtatttacgg agacagaaaa aatcaaaggt cctaaggttg aatttttctc
    13981 tcctaaagaa ggagaccctg ctgattacgt caaggtagtg atggaagacg gagaagaagt
    14041 gactattaca aatacatgtg cagccaatgt tcttggatta gtgcaagaag aacatttttc
    14101 ttatggaaat gtcccatctg cacgaaaagc catacaggca ttagcagact attgtcatac
    14161 gacggttgaa gacatcgcag agcagatcat ggaaaaatcc tatgctaaga ttgaaccggt
    14221 gattttagaa ttggcagaca agtatcactt ggagaaagat cagatttctc ttgtaggtgt
    14281 agggggcggc gctgcctcat tgattactta ctttagtaat aaaatgggtg tgaaatattc
    14341 gattcctgaa aacgccgaag tgatttcttc tattggtgta gcacttgcaa tggtccgaga
    14401 tgttgtagaa cgtatcatcc cttcgccaag taaagaagat atacgatcgt tgaaaaatga
    14461 agcgatgaat aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga
    14521 aatcgatcca catacttcaa aagtaacggc tatcgctact ggttctacgg aagtgaaagc
    14581 aaccgatcta accaaggaga tcacaacaga agaagcacta gagcttgctg cagaggatat
    14641 gcgattaaat aaaaatgaag tttgcttatt ggaaaataca cctttctttt atgtatgcgg
    14701 cgaacagaat cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa aaggatttat
    14761 caaggtccaa cgtggacatg cttcatgtat gaaaacaact gcagccaatt atatgacagc
    14821 tgtcgaacaa ctttgggaag atatggcagt ttatcaaact gaattgattg cccgaccaga
    14881 attttatctc tgcttaggag caagagtctc tgattttaca gcgacggatc tggaacaact
    14941 acagcttttg atggatctgg aagtatctac tatggaaccg gaggaagaag tgatcgtcgt
    15001 tgccggaaat atcaaacaaa cataagttag aagagttact aaacgaatta caacagatcc
    15061 ccgaagaaat ttggggattc taccagtttc ggcgagattt attttggaaa aagattcctt
    15121 tgtccaagca gaaaattctt atacaacaat caatcgattg tggtatcgaa acggcatgta
    15181 gcatcaaaaa aaagtaccct tttgccgatg taggagaaat ctatgaacaa atggctgttc
    15241 caatcgtttc ctgtgaatca gagcaaataa acgaacgaat cacttttgca acttatgcag
    15301 aagatgaagg gatccgattg atgacggagc cgttagaaaa attaaaatgt tcaggactag
    15361 cttcgatctc taaagaaaca gcgcaagctt tgattattgg acatgaacta tttcaccaca
    15421 tcgaagcttc tgttaaggga atttatacgc aaagcgaaaa aattgtgctg tggcggttgc
    15481 ctttttatac acatcaatca aacattcgtg cattgagcga gatagcagcg atgagttttt
    15541 caaaagagat gaatcaaagc gatttttcgc cttatgtatt agaggcagtc ttactttggc
    15601 catataatga aattcgtagc caagggatct tagaagaaat aaaagaaata gaaaaaagat
    15661 gtacggagta tgatttagat tacaaataaa gagactgtga caaaagtcgt gtcacagtcc
    15721 tttttttccg aataaacggt gttctcctgt caactcctcg gtcacaagtc aaaatattca
    15781 acaaacatga gaagctgttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc
    15841 tcacgacctc ttggaaaaag gtgttcaaaa gcttgcatct gcggtaataa gaacagccaa
    15901 gttaaaaaca taagaagcta tttttacttg gctgttctta ttacttgatg cagggcagct
    15961 ttttcacaac ctctcaataa acggtgtgca atcagctgac cctcggtatt agctcaaaaa
    16021 aaacggcaaa atatgaggaa ctattttgcc gtttatttct ctaatactca ggtcaaaacg
    16081 ctgattccaa aacctcttat ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt
    16141 aattatgaat cacgtattct tgctcctcga taatcatatg taaatgcatc gtgcaaagaa
    16201 atccgacttc atcaaagtta tgatcaatca ttgattgaag cattgtctga tgttcatcta
    16261 atactcgacc ccagtctaat tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt
    16321 gcgtactata actttctagc caatcccaaa tttctcgttt tccagcaatt tcataacaag
    16381 ttcggtgaaa atcagtatcc aaatcaaaaa aagctttttt attttgagcg atcactgcct
    16441 gatcggtttg ttctagtatt ttttctagtt tagcttgatc ttgcttggtc attttcgcac
    16501 tgcattcttg caggatcggt ttttccaact tttcccgcgt ataacgggct aatgaagcag
    16561 atcggatatc gatttttgaa acataagtgc cggactgtgg aatggttttt acaagctctt
    16621 gattgcgcag ttggatcagt gcttctcgaa tcggtgtacg cccgatagct agttgttctt
    16681 ctaacacttt atcggagatt ttacttccag gtgctaactc ggaataaatg atttgtttac
    16741 gtatagattc ataagcttgc tgttgcaaat tattgataac catttaacct tcctttctat
    16801 gcagatataa tctattataa cataaatcgc ttacaaatcg tgtgggcata aaaacacagt
    16861 ctgcaaaaaa agccctgata ggcatgccta ttagagcctc gaaaagaaaa atattgaatc
    16921 aatctggtcg tacctcttgg atcggtttga cacgaccgcc gattctttta gcaaaagctt
    16981 cggattcttt tttgtctttg aaaatgagca gattgtctaa gtttttttcc cgagtgcgac
    17041 agccagtttt atcgatgtaa ccgtgcatta ctttaacgac atacatgggg atcacctgct
    17101 tttttctttt cattatacga actactcgac acaatacaag aaaaatgctt agacggtatt
    17161 tattaagaat ttacatcatc caaagaagcg tacgcttgga ataactgttc taactccgga
    17221 ttgactttgc tcagcttcac taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta
    17281 ctgccagtag tttttaattt tttgtcttca gttcccacaa tttcgtagct aaaatccaac
    17341 cgtgttcccg tatacttttg gatagttggg tggatgctca ctttttctcc gtaatggatc
    17401 atttctttat agctgcaatt tacttccaga acggggatga tgatcccaga ttcttcgatt
    17461 tttttatagg gaaaccctag atgtaccaat aaggctaccc gagcctcttc aaaccagcgg
    17521 atataattgc tgtgatgaat aatccccatt tgatctgttt cataataaaa aggttctcgc
    17581 acataacctg taaatttctc catgactcat gcctcgtttt ccattgattt tttacaaaaa
    17641 taattcatcc acttcggaag tgcttcgctg atttgtgtcg gcaaaacaat atattgttct
    17701 tttgccagct ggtctccaat gaaactatga agataaacag ctgctgcaat cgtttggtca
    17761 ttttgaggaa actgggctaa aaatcctgtg atcatcccag ctaaagtgtc acccatgccg
    17821 cctgttgcca tagcagcaga acctaaagga ttggtgtaga agtgctggtg accataaatt
    17881 tttgtctcat gacttttcaa aacgagcaca gcaccgattt cctgttgctt ctttttgttt
    17941 tctttatccc gttgttcttg tatcggtaga gaactcaacc gttgccactc catttgatga
    18001 ggtgtaagta cagtttgttc gggatagggt agcaaaagat gattagcaac aagtaaactt
    18061 aaagcagagc catcaatcac cagccattgt tcttttgatt gtttttctag tacttgtttg
    18121 aaacgttcca aactcttaga agaagtaccc aatccgggac caatcaagag tacatctgct
    18181 tgctttatta cggaaagaaa ctggtcagta tccgtccaat ctacaatcat tgcttctggc
    18241 agatgactgt gaagagcact ccgatttttt tcatcagtta ctacagtcgt gagacccgct
    18301 ccactgtgta cggcagctaa agcactcata atgattgctc cgccatattg ttcattgccg
    18361 ccaatcaaaa cgatacgtcc aaatgttcct ttatgtgaaa tctttggacg aggtgtgatg
    18421 actcttttca ctagttgttc gtttatcagc atcgaaaaac ctccttttac tcattatagc
    18481 gtatattttt tttagagaaa aacagataag aaaaggaatt gtcagatttt ttgaactatt
    18541 tttttctgta atttgtggta tcatgaacac gaagacttaa ataagtggag gattaacttt
    18601 atgacaattg attggcaaaa agaagtagaa gcacgtaaag aagacttgct tgaagacttg
    18661 aaaaatcttt tacgtgtcaa cagcgagcgc gatgattcaa aagtaacacc agatgcacca
    18721 tttggacctg gacctcgcga cgcattgaaa catatgttag cttatggcga acgtgatgga
    18781 tttaaagtaa aaaatgtcga taactatgca gggcatattg atcttggcga aggagacgaa
    18841 acgctaggga tatttggaca catggatgtc gttcctgcag gagatggctg ggacacagat
    18901 ccttatgaac cagtaatcaa ggatggaaag atctttgcac gcggatcaag cgatgacaaa
    18961 ggtccaagta tggcagctta ctatgcgatg aagatcatta aagaacttga cttgaaacta
    19021 tcaaaaaaag ttcgcttcgt agtcggcagc gatgaagaaa gcggctgggg cgatatggct
    19081 tattatttcg aacatgaaga agaaccagat tttggtttct caccagatgc tgaattccca
    19141 atcatcaatg gtgaaaaagg aaatgtttct ttagctttac gcttcaaagg cgacaatgca
    19201 ggagattatg tcttgaaatc ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca
    19261 gcaactgcag cacttcaagt accaagtgca gatgcagcta tcgcgatgga agaagcattt
    19321 taccaattca tcgaagcaaa tccagtaagc ggaacgattg aagcggacaa tacgtatgta
    19381 aaaatcgaat tagttggtaa aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca
    19441 ggttctttct tggcattgtt cttagataac tatgaattcc taggatctgc taaacaattt
    19501 atccatgtag cagctgctta tgtccatgaa gatttttatg gagaaaaact aggagtcgct
    19561 tatgaagatg aaaaaatggg caaattgaca atgaatgctg gactatttgc ttttgaagaa
    19621 aatggtacag aggaagcgaa cttcatcaat atgaatttcc gttttccaaa aggtgtaaca
    19681 gtagacggat tgcaaagtga catcgaacaa acagtggggc aagaaggcgc aacagttact
    19741 cgaggtgctc gcgtaatgga accacattac gtaccaatgg atgatccatt ggtggctaca
    19801 ttattgcaag tctatgaaga ccacactgga gaaaaaggct acgaacaaat catcggtggc
    19861 ggaacttacg gacgtttgtt gaaacgtggc gttgcttatg gagcgatgtt cccaggatat
    19921 actgatacaa tgcatcaagc aaacgaattt atgagtcttg atgacttgtt cagagcaaca
    19981 gcaatctatg cagatgccat ctatcgtttg gcgaaataaa aatactaaaa aactccggct
    20041 atctctgaca gctggagttt ttatgtttaa aaatgaatta gtacatcggt attcttaaaa
    20101 gtaatttagt tcgtcttttg atttctcagg agggatcaaa aaggcgaaag cactaagtat
    20161 agcaaaactt gtgatgattt gcgtacgata cgtaaaccaa taatgaatct gtgagtgatt
    20221 agctaaaata ttgtaccaaa tatacggcgt tacagcagct aaaacaaaca aaactagaag
    20281 ttggaacgga cgccagctat ttcgtttacg ataagcaaaa tagagaaaaa caagacaagt
    20341 aatcaatagc aataatattg ctacttttgg atacatcaat ctaatattgc ttttcagcat
    20401 ctctaatcga tgcaacgggt atttgtcgtt tccttccgtt cgaaagagga tttggttgat
    20461 tgcatctttt acaatgtttt tgttcaatat gatcgatgat aggatccatt tagccaccca
    20521 tgtcaaacca tagcctaatc cccaaaagat accggtaaaa acaaaactgc ctacttgttt
    20581 gctcaatgaa ctgcgtttat ccattaaata cttatttgtt acataataaa taatgatcac
    20641 aggtatcccc caagagatga ccggtgttgt caacaagtca aaaaagttag ctaaagaacc
    20701 aatgacaaag aaatacaaga ataattgttg gttggtgttg gagagttgtg gttttgttaa
    20761 aaatagtaat acagacaata aagtcaaaat ccaaatattt gaaaattgca tactgaagaa
    20821 aaaggttgca gcatttccta tagcaagcga aatcaacaag gctgtcgtta aatagatgtc
    20881 gctacacttg gttaaaaagt agctcgtcaa tccaatcagc agaaaaagaa tggctgcgta
    20941 aatcattcga atactcccca aactcatccc tagcagtaaa ggtcgtaaaa aaagctgata
    21001 accatgccaa taacggggat aatccgccat agacatcgcg ttctccaagg agttcaattc
    21061 aggatctgct tttgtttttc gtatcatcaa atgatcagta aaattatcta accgagtgcc
    21121 tagcatattt ccttgattga tagaaggata cattccttct ttttcaacca tctgtagaga
    21181 tttttctgca ttcgattcaa tagagtgctg cggaatgaaa aaagtaattg tcagtagtac
    21241 gcaatagcta gcgatcaaga gaacaaaagc aaaaaatatc tttttaaata gtttcatgag
    21301 tatctcctat atctgttttc attttttctc ttttatctag taaaaaagga gagaggatat
    21361 aaacaggtag acctaatgct aaaacaaaca cataccgtaa actaaaagcc acaggagtag
    21421 caatcataat agtagcaaaa acaagcatac cgggaagaaa aaggagtaaa taccgatatt
    21481 ttttcttaaa gattgctaaa agtgcactgc cgatagctaa ccacagtaat gtgccggaac
    21541 caaagaaatc acgagcatcg atgatttttt tcattcctga caatccagta agatgttcaa
    21601 tcaaatctac ttgcttcata ccataattat tcgtgtttac acgagtatct aaaaagccgt
    21661 aactattttt tgtaccaggt acccagaacc cgaacgtttc taaaatataa gcatctgtat
    21721 agatttttat attacttggt aaatttcttg cccatagtga tagaaattct tttttatgtg
    21781 tattcaaaaa ctcattgtta aaatttttat gccacttgat tgagtctgca agactaggag
    21841 aatacgcttc ataatctttt atcgggagta gctgattcat gaatgcttta tctttcttag
    21901 acaagtcacc atccataaca atagttctag aaatttgctg caaaggaaca cctaattgtt
    21961 cagcagtttc tgtaggaata gcataacgag ccatcaacgg gtggatgagt agtccagcaa
    22021 tcactgtata acccagcacc acttgaaaga atgcttgata ccttttttgt ttcaaaataa
    22081 tcaagccgca aatcaaacct gtgccggtta ctacataaat cccattattt cttagatagg
    22141 tggtcagtaa agcaaggatg attacgtaaa tctgattgag aatcgttggc ttttctcgtt
    22201 gaaaaataaa gtcaaaaacc gctaaggaaa ataaaaagca agcgatagag aacaaagtat
    22261 ctttttgcat gttcaaggcg tagattggaa aaaccggtgc aaatgcaaaa tagcataaac
    22321 taatgagtac cggaaaaaaa tggatgtttc tttttaacag ccagcaaaga gaataactta
    22381 tcgctgaagc tgttatcaat gtttgaaaaa tcgaaaataa aaatacaccg tgattgatcg
    22441 tatcagttgg taaatcatga acaaatagct tgaccagtaa ggtgaataag actggatgat
    22501 gattgcttat aggaacatca cccatagatt gggcgaccga acttaacgaa tcaggcaaaa
    22561 ccactccagg atacaatgcc aacagataag gaagccaaca cagaactaaa aagacagtaa
    22621 agaataaaaa atattttctt gaagagaaag cctgtgtgtt ttgagcagta aaaaatgttt
    22681 ccagttttgg cattgataaa taaatcgatt gtaaaaataa aaaagaatac acaaaaatgg
    22741 agaaataaac aagaaagtaa aaaatacttc catcagcaat cgttgtcttt gtatagtttg
    22801 ctgagacata ctgaaattgt atctgtctgc caatagaaag tgtaagagca aaagctgcag
    22861 aaaaaaacaa tcgggcaata gctgcaactt tgttttttat tgaaaacaac tctattccat
    22921 attgcttaaa agcaagtagc aaaaccatgg ctaaaccaat gattactgtt ggattcaaag
    22981 ttcctagacc ataagaagta aaaaaagcaa aacctgacgc aaataaagtt gcaaaaaaag
    23041 taggaaatag attctccttt ttcttatgca taaaaaaggc ctcctacttc atttctttat
    23101 tttgatattc gtggtagaca tggtataaat tcaattcata ttctttcttg gcatttgtga
    23161 cgaccgtatc caaaatcaac cctgttacta atgaaagaag ggctaaagtc atcaatccag
    23221 tacttaaaat cgcagaaggg atgcgtgtaa taaagcttgt cagcatatat tcacgaatca
    23281 caggaatccc catgaccaat ccaaataaaa agaagaagat ggtccagatc ccaaagaaaa
    23341 gcaaaggttt ataatctttg cacattttga tgatcatcat gatcactttg aatccgtcag
    23401 agaatgtatt caattttgat tcgctacctt caggacgatc gcgataatcg attggaatct
    23461 caacgaactt gaatttttta tctaaagcat gaatcgtcaa ttctgtttcg atttgaaaac
    23521 cagaactcat aatagggaag cttttgacaa aaattcgatt gaatcctcga tagccggtca
    23581 tgacatcgcg aatttttgtt ttatataagt aagtaattgt atttttaacc agattattcc
    23641 caaaatcatg gaaaggacgc ttattttcat caaaataggt tccatttgat agacgatcgc
    23701 caatgaccat atctgcttcg ccagaacgta atttctcaag caaaccatgt gcagcttctg
    23761 caggataagt atcatcacca tctaccatca aataataatc ggcatcaata tcaaaaaaca
    23821 tttgacggat tacattccct tttccttgac gtggttcttt ctttacgatt gctccgcctt
    23881 gaacggccaa ttcgtatgtt cgatcggtcg aattattatc atagacataa atatctgctt
    23941 ctggtaattc ccttttaaaa tcttcaataa cagttgaaat tgtcgcctct tcgttataac
    24001 aaggaatgag cacggcaatt ttttcacgtg attccatttg tcactctcct tatataataa
    24061 aactaatctt atttcaataa taccgtataa taactacaaa tactagctat tccaagagga
    24121 aatagcccta tttttataaa aatcacccac tataaaagaa ggataggata cctctctttt
    24181 agatagtggg tgcgtattcg ttatttcatt tttttgacgt attaatctat tgcgcccttt
    24241 tttcttgaaa tgtctaatac ggttcctgta tgagcatctg ctaaaaattc ataataaaca
    24301 gtttcgccat cttctatccg ggaaagccca ccccgataag cactcgaccg aacggcgaat
    24361 ttccgaatag gttctttttc aaaagagatc caagcacctt cgattgcgcc ttcttttaaa
    24421 aaagatgttt tgatattttc aagcacttgg tctgctgata acactttttt gcgatgatac
    24481 caaatcgttg aagctacccc actcaataat cctagaccga tcccaacggc taacccgcct
    24541 ttaaaatagc ttaattcgct ttcttcatac atgtggatat acctccttct tctcacttgc
    24601 tactctttgt gtttatttta ccacactctc atattttctt tgaaataaat aaccgctacc
    24661 agaaaaaata agctataata aatacgatcg gtctgcagaa aaaatcatat tttatccgca
    24721 gatcaatata agtctgagag gagtttagtc tgaaatttag acgaatagca tatggaagaa
    24781 aaaacatttc aacgaatcaa agagttaaca gaattacaag gaacaagcgg ttttgaacaa
    24841 gacgttcgcg cctatatgga aagtcatatg gaaccgctgg tagatgagtt acagttagat
    24901 ggtttgggcg gcatttttgg gctgcgccac catgaagaag cggatgcacc tcgtgtaatg
    24961 gtcgcagcac atatggatga agtcggattc atgctgacac aaatccaaga aaacgggctt
    25021 tttaaagtcg tacctctagg cggatggaac ccttacgttg tttctgctca acgttttaca
    25081 cttaaaacct ctactggtaa gaattatcca tgtatttctt cttccgttcc cccacattta
    25141 ttacgaggaa caagcggaca aaaatcagta gaagtaacag atattttgtt cgatgctggg
    25201 tttgaatcaa gagaagaggc aatgagttac ggtgttcttc caggagacac aatcgttcct
    25261 tacgctgaaa cgatcaaaac agccaacggg aagaatatca tcagcaaatc gtgggacaac
    25321 cgctatggct gtacaatggt attagaagct ttagaagcgt tacaaaatga aacattaggt
    25381 catacattga ttgcaggcgc gaatgtccaa gaagaagtag gcctgcgtgg ttctaaagct
    25441 tctgtcaata aattcaagcc agatctgttc tttgcagtag actgctctgc agcagatgac
    25501 acagtgacga aaaatggcac attcggacat ttaggagaag gtacattgat gcgtatccaa
    25561 gatccaggtt tgatcatgct tcctcgtttg cgtgagtact tgctagatat cgctgaaaca
    25621 aacaacatcc cttatcaata ctttgtttca aaaggcggta cagatgcagg tgctgctcat
    25681 acacaaaatg aaggaatccc aagtacagtg attggcgtag tgggacgtta tatccatacg
    25741 catcaaacga tgttcagtat tcgcgatttt gaagctgcac gagaaatgtt gattcaaaca
    25801 ttgaaaggtt tagataaatc aacagtgaac acgatcgtat acgggaaata acgcgatagt
    25861 gaaactataa aatctggcaa aagcaacctt ttgtcagatt ttttttataa aaagaggatc
    25921 aatgtttgtt agttcgaaag aaaggtgtat aatttaattg aaaaatacat aaacagaaag
    25981 tagaggaaaa agaatgatta tcccaaatag tttagaagaa ttagcaggct acgtagaaac
    26041 tggaaaaagt gtatttttct ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc
    26101 gcaaatgcct gaaatcgaaa atgacttttc tgattggcaa tttattgaag tagaccgtga
    26161 caagtacata gatgtagccg ctgaatggaa catttttggt atcccaagtt ttgtcgtgat
    26221 ccaagacgga aaagaattag gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat
    26281 cgaatcgttc ctagaaagtg taataaaagg ctgacatctg ttaggagggg tcaagatgaa
    26341 tcaagaatat aagaacattt tagtaggtat tgacggtagt gaacaagcac ttgctgcttt
    26401 caaaaaagcg ttagaagtgg ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat
    26461 cgatcagcaa ttttataatt ttatgggcta tgctcctatt gatcaaaatc tagtcgatca
    26521 gcaaactgaa acagccaagc aaatgatcga agaatgtaaa gaatatggaa aatctgttga
    26581 ttacacaaat atagaaggaa ttattgcata cggctctgtt cgtgaatcta tggcagtcaa
    26641 attgccaaaa aaatatgcaa tcgatctaat catggttggg cagtctggac taaatgcagt
    26701 cgaacgcttt atgacaggaa gtgtagccag ctacgtcata cgtcattcac cttgtgatgt
    26761 cttgatcgtt tctgacgaag aagaacagcc gagtaattaa gcaagtggaa cttgtataac
    26821 ctttttaatc aaacagtaaa gggactgtga caaaagtctt gtcacagtcc cttgttacga
    26881 ataaatggtg ttccagaaga agcttcttcg gaaataaagc atgaaaaaaa tctcatgcag
    26941 tgaatgcaat tttttgttaa gataaatctc agcaaaggat aatggaggaa agaacatgat
    27001 ttttgcttat aacaaagcac acgtcggaga cactttgatg gttatcatag ccgacgataa
    27061 aggaacagaa aacactgtcg aacgtaaatg gaatgttgct cggatcaaag atgaacaagg
    27121 tcaagttgtt gcttggaact ttttccatat ctccgatcat ctaacaattg aagggaacgg
    27181 ccaagtaagc ttaacagatg aagaattagc gaaattgaat cgattgatta aagaagcagg
    27241 attcgaagaa cagcttgaag ctgatcatga accgaagatc gttgtcgggt atgtaaaaac
    27301 atgcgtcccg catcctgact ctgaccattt atcgattact gagacagaag tggacaatgg
    27361 gacagtattg caaattgttt gcggtgcacc aaatatcgaa gctggacaaa aagttgtcgt
    27421 tgcaaaacca ggagccatga tgccagatgg aatgatgatc tggccaggaa ctttgcgagg
    27481 ggtagccagc tatggaatga tttgttctgc tcgtgaattg catttgccaa atgctccaga
    27541 aaaaaaagga atcttagtat tgcctgagga tgccgtagtc ggagaagtat ttccaaaata
    27601 aagaggttgt gacaagtctt tgtcacaacc tcttttttat taattttcac tattttgatt
    27661 ttgttttaat gccgattgat cgatcgtcaa ttcaacagta gttgtttttt ctttcgagcc
    27721 ttcatagtaa gtgattttga cagaatctcc cacttttttc ttatacaaag cagattgaag
    27781 ttctacacca gaagaaactt ctttgtcatc gatcttagta atgacgtcgt attgtttcaa
    27841 cccagctttt tctgccggtg tagctgtttg gacatttgtc acgatgacac cgttagttac
    27901 agatgaagga atcttcaaga tttgttcttg ttgctgagta gaaacggcag ataagtcgac
    27961 cattgtgata cctagagctg gacgtgtcac ttttccgtct ttttccagtt ggttgatgat
    28021 attcactaca tcgttacttg ggatggcaaa tcccattcct tctacgctta cattagaagt
    28081 cgattcagaa gtacttgcga tcttacttga gttgataccg ataacttgtc cttcgatatt
    28141 cactaaagga ccaccagaat ttcccgggtt gatcgctgca tctgtttgga tcgcattgat
    28201 attgactgtt tcgttcgatt cattcgtact tgttacttgt cggttcaagg aagaaatgat
    28261 tcctgaagtg acagaattcg catattcaga tcctaatggg gaaccaatcg caatcgcagg
    28321 ttcaccaacc ttcaaagcac ttgagtcccc aaaagaggca accgtttcga ctttgtctga
    28381 attgatcttc aacaccgcta aatctgaata ggcatctgtt ccaactaatt cagcttttac
    28441 cttcgtaccg tctttcatca tgacttccaa accttgctgt ccatctacta cgtgattgtt
    28501 tgtgactaca taagcagtat tcccgtcttt tttgtagatt acgccgcttc cttcactata
    28561 agcttccaaa ttactatcat ctgagctgct actttcttct tgctgcccga ataatccgcc
    28621 aaaaccgctt gattggtttt gactttgtaa gttgataaca gaaacgacag cgtcttgaac
    28681 tttatctacc gctttggtga tatcagaatc tacattgact ttgacatttt ccacaactgt
    28741 ttcaccggca gaattttgat tgccgcttgt agcagtagaa ctattgttgc cagtgcccat
    28801 cgctgcataa aaaatgccag ctgtcagtag tcctccaacg atccctccaa caagccccag
    28861 acccaactta cgccagaggc tattattttt attttttttc atctttggtg tcacattttt
    28921 tctatccatg tgttcctcct ccaattacat tcatttatct actgttagta tagtcttatt
    28981 tgttgacttt agtctacttt gagactttga actttttatg aaaaacttta ggaaaaatca
    29041 ttgttttcag atgaaaatat ttgattttct ttttccacag atattgtgta aaactacgcg
    29101 gataggattg gaaaaataag ggataaaaca tatccacagc ctgtgtataa agtggataaa
    29161 ttcttataaa acgtatgttt gcctaatgaa tgtgatgaaa taaggtaaaa caacgctgtt
    29221 ttctgtggat aaacctgtgg atgttgtgca taactgttgg ggaaatcttt ttaacaaaaa
    29281 tagtcaaaat aaacatatag acgaaaaaat tgattataat aaacaaagtg agcgtgaaga
    29341 tcaaaggaga gaaaacatga atatcaaaat tatttcagta ggaaaattaa aagaaaaata
    29401 cttgatccaa ggtatcaatg agtatgtcaa acggctgaat gcctatgcga aaatcgaatt
    29461 gatcgaagtt cctgacgaaa aagccccgga gaatttaagc gaagcgcaaa tgagacaagt
    29521 aaaagaaaaa gaaggagaac ggatcttagc gaaaatcaaa gaacaagaat acgtctatgg
    29581 cttagcaatt gaagggaaaa atcccacaag cgaatctttt gctaaacaaa tcgaccaatt
    29641 aggtatccaa ggaaaaagcc accttgtttt tgttatcggc ggttctttag gactcagcga
    29701 agccgtcatg aaaagaagca atgctcagat ctcttttggc aaaatgacgt atccacatca
    29761 attgatgcgc ctgatcctcg tcgagcaaat ctaccgtgcc ttccggatca acgcgggggc
    29821 tccataccac aaataatgat attttttaag agaaaaatag cataaaaatc tagttatccg
    29881 cataaaaact ggacttatca cactttatca aggtcaaaac cactcaattt actactaatt
    29941 tactacttat gaatgagct
//
LOCUS       DAXWLG010000014        29919 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXWLG010000014 DAXWLG010000000
VERSION     DAXWLG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.014"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..808
                     /locus_tag="ACGE4T_000505"
     CDS             <1..808
                     /locus_tag="ACGE4T_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8385814.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1161..2009)
                     /locus_tag="ACGE4T_000506"
                     /pseudo
     CDS             complement(1161..2009)
                     /locus_tag="ACGE4T_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2162..3274
                     /locus_tag="ACGE4T_000507"
     CDS             2162..3274
                     /locus_tag="ACGE4T_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8385815.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="ACGE4T_000508"
     CDS             complement(3352..3645)
                     /locus_tag="ACGE4T_000508"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFF8385816.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="ACGE4T_000509"
     CDS             3813..4127
                     /locus_tag="ACGE4T_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFF8385817.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="ACGE4T_000510"
     CDS             4148..6127
                     /locus_tag="ACGE4T_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8385818.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="ACGE4T_000511"
     CDS             6140..6466
                     /locus_tag="ACGE4T_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFF8385819.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="ACGE4T_000512"
     CDS             6482..7000
                     /locus_tag="ACGE4T_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385820.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="ACGE4T_000513"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="ACGE4T_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFF8385821.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="ACGE4T_000514"
     CDS             complement(8436..9941)
                     /locus_tag="ACGE4T_000514"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFF8385822.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="ACGE4T_000515"
     CDS             complement(10033..10623)
                     /locus_tag="ACGE4T_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8385823.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="ACGE4T_000516"
     CDS             10802..12448
                     /locus_tag="ACGE4T_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFF8385824.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="ACGE4T_000517"
     CDS             12497..13216
                     /locus_tag="ACGE4T_000517"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8385825.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="ACGE4T_000518"
     CDS             13695..14642
                     /locus_tag="ACGE4T_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8385826.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="ACGE4T_000519"
     CDS             14654..15574
                     /locus_tag="ACGE4T_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8385827.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="ACGE4T_000520"
     CDS             15615..17072
                     /locus_tag="ACGE4T_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8385828.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="ACGE4T_000521"
     CDS             17417..18031
                     /locus_tag="ACGE4T_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8385829.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="ACGE4T_000522"
     CDS             18024..19748
                     /locus_tag="ACGE4T_000522"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8385830.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="ACGE4T_000523"
     CDS             19745..21196
                     /locus_tag="ACGE4T_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8385831.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="ACGE4T_000524"
     CDS             complement(21669..23822)
                     /locus_tag="ACGE4T_000524"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFF8385832.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIRQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="ACGE4T_000525"
     CDS             complement(23824..24870)
                     /locus_tag="ACGE4T_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8385833.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="ACGE4T_000526"
     CDS             25020..26309
                     /locus_tag="ACGE4T_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFF8385834.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="ACGE4T_000527"
     CDS             26322..29024
                     /locus_tag="ACGE4T_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFF8385835.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29009..29866
                     /locus_tag="ACGE4T_000528"
     CDS             29009..29866
                     /locus_tag="ACGE4T_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8385836.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
BASE COUNT         9724 a         5172 c         6097 g         8926 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat atgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcttatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgattcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggcttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atacttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggac ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttagaa aaattaagta tccctaccac
    29101 aacaccagaa gaaacattgg atcaggtatt tgaatttttt gatcgttatg aattgaaggg
    29161 aatcgggatt ggctcattcg gcccgattgg tatcgacaaa acaaaagata attacggata
    29221 cgttttggca actcccaaaa aagggtgggc acaattcgat tttcttggca caatcaagga
    29281 acgctacaat attcctgctg cttggacaac ggatgtcaat gctgctgcat atggggaatt
    29341 gaagcaagga gcagcacaag gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat
    29401 cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg atcgcacatc ctgaaatggg
    29461 acatatttgg gtgaaacgcc atccagatga ccattatgaa ggtacttgtc cttatcataa
    29521 agattgttta gaaggattag cagcaggacc gtctattgag gcacgcacag ggatcaaagg
    29581 gcaagatctg ccgcaagatc atcctgtttg ggctattcag gcttattata ttgctcaagc
    29641 actgatcaac tatacgttga cgttagcacc tgaaaagatt attttaggcg gtggggtaat
    29701 gaatcaggat catctccttc aaaaaatccg tcagcaattt gttgaattga tgggtggtta
    29761 tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg ggactgccga atgaaagtgg
    29821 gattatcggt agtttgctgt tagcagaaca cgaacatcaa aagtaagcag acaaattaca
    29881 caaaaagaac agagtcttat cattatctgc tgataagac
//
LOCUS       DAXWLG010000015        28309 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXWLG010000015 DAXWLG010000000
VERSION     DAXWLG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.015"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACGE4T_000529"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACGE4T_000529"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8385837.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACGE4T_000530"
     CDS             2370..3848
                     /locus_tag="ACGE4T_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8385838.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACGE4T_000531"
     CDS             3814..5145
                     /locus_tag="ACGE4T_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8385839.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACGE4T_000532"
     CDS             5259..7811
                     /locus_tag="ACGE4T_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFF8385840.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACGE4T_000533"
     CDS             complement(7818..9257)
                     /locus_tag="ACGE4T_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385841.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACGE4T_000534"
     CDS             9232..9414
                     /locus_tag="ACGE4T_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385842.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACGE4T_000535"
     CDS             9603..9965
                     /locus_tag="ACGE4T_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFF8385843.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACGE4T_000536"
     CDS             complement(10152..11009)
                     /locus_tag="ACGE4T_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8385844.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACGE4T_000537"
     CDS             11129..12040
                     /locus_tag="ACGE4T_000537"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8385845.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13693
                     /gene="mdcA"
                     /locus_tag="ACGE4T_000538"
     CDS             12047..13693
                     /gene="mdcA"
                     /locus_tag="ACGE4T_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFF8385846.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13795..14094
                     /locus_tag="ACGE4T_000539"
     CDS             13795..14094
                     /locus_tag="ACGE4T_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFF8385847.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14084..15760
                     /gene="mdcD"
                     /locus_tag="ACGE4T_000540"
     CDS             14084..15760
                     /gene="mdcD"
                     /locus_tag="ACGE4T_000540"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFF8385848.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15764..16357
                     /locus_tag="ACGE4T_000541"
     CDS             15764..16357
                     /locus_tag="ACGE4T_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFF8385849.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16381..17457
                     /locus_tag="ACGE4T_000542"
     CDS             16381..17457
                     /locus_tag="ACGE4T_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8385850.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17482..18351
                     /gene="mdcB"
                     /locus_tag="ACGE4T_000543"
     CDS             17482..18351
                     /gene="mdcB"
                     /locus_tag="ACGE4T_000543"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFF8385851.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18462..19226
                     /locus_tag="ACGE4T_000544"
     CDS             18462..19226
                     /locus_tag="ACGE4T_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFF8385852.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19715..21808
                     /locus_tag="ACGE4T_000545"
     CDS             19715..21808
                     /locus_tag="ACGE4T_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8385853.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21833..22135
                     /locus_tag="ACGE4T_000546"
     CDS             21833..22135
                     /locus_tag="ACGE4T_000546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8385854.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            22162..23451
                     /locus_tag="ACGE4T_000547"
     CDS             22162..23451
                     /locus_tag="ACGE4T_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8385855.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            23470..23877
                     /locus_tag="ACGE4T_000548"
     CDS             23470..23877
                     /locus_tag="ACGE4T_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8385856.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            23900..24769
                     /locus_tag="ACGE4T_000549"
     CDS             23900..24769
                     /locus_tag="ACGE4T_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8385857.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            24771..25391
                     /locus_tag="ACGE4T_000550"
     CDS             24771..25391
                     /locus_tag="ACGE4T_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8385858.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            25841..26446
                     /locus_tag="ACGE4T_000551"
     CDS             25841..26446
                     /locus_tag="ACGE4T_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8385859.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            26581..27033
                     /locus_tag="ACGE4T_000552"
     CDS             26581..27033
                     /locus_tag="ACGE4T_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8385860.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            27070..27567
                     /locus_tag="ACGE4T_000553"
     CDS             27070..27567
                     /locus_tag="ACGE4T_000553"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8385861.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            27585..28295
                     /locus_tag="ACGE4T_000554"
     CDS             27585..28295
                     /locus_tag="ACGE4T_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFF8385862.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
BASE COUNT         9305 a         4840 c         6025 g         8139 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 ttcttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaccagt
    13381 gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg cttatttgta
    13441 caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg gtgttacacc
    13501 tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg gcatcgtgaa
    13561 attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat tagctgcaca
    13621 aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa tgaaattcag
    13681 aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag atggaagaag
    13741 aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag atccatggaa
    13801 caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca tgtaggagtg
    13861 gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga atctactgta
    13921 aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt aacgagattt
    13981 tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc tacaccagga
    14041 gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa atagttttgt
    14101 agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg aattgattga
    14161 tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc ctgaaagtga
    14221 tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag taatcgctat
    14281 ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa tcgttgctgc
    14341 acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag tcattgtact
    14401 agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta tttcagaaat
    14461 cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga ttccaggacg
    14521 tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt taatcgcaac
    14581 aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag caggcgttcg
    14641 agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc gtcaacgggt
    14701 agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca aagctgctgt
    14761 catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag cattttattt
    14821 atcattactt gattctttag atctttcaaa accaatggcc attgacgatt acaatgaagc
    14881 aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag gaaatgaacc
    14941 agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga aaaatccaac
    15001 ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag aagccatcgt
    15061 gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg gtgaagttgg
    15121 tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag aagataaaga
    15181 caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag catacggcta
    15241 taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg cttatgcaaa
    15301 actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta tttcaggtgc
    15361 atttttagca catggattac aatcaagccg cttgattgct ttaaacaata aagaaattac
    15421 cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg ttgctgaaat
    15481 cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt atacaaaact
    15541 aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag aacaaaatgt
    15601 cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata caaatgacac
    15661 gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg tcgaaacaaa
    15721 taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc aagcacatga
    15781 tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat gggtcgttct
    15841 aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag aaatactacc
    15901 gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct caaaacatgc
    15961 cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata gacaagagaa
    16021 aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat tatcaggatg
    16081 taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc cggcagtaaa
    16141 agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat tgccaattca
    16201 ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag tgactcggca
    16261 aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc tattgaaaac
    16321 agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag agagaaagag
    16381 atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt atcagctgta
    16441 acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca cagcgcaaca
    16501 aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc aatgtttatt
    16561 tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg ggaatttttg
    16621 aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc ttatctttgg
    16681 ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt tttactgaaa
    16741 tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct gcagatccaa
    16801 ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa aaaatatgta
    16861 tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt ctatcaaaca
    16921 caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt tgttgctagt
    16981 ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca atatttggac
    17041 agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc tgtccgttca
    17101 ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa tacgatccaa
    17161 agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa aatgattgta
    17221 aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta tcgtacgtat
    17281 ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag ttggctgcag
    17341 ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg tgcatttgct
    17401 ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag caagtaatag
    17461 taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt gactcagcaa
    17521 ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc aaaacctgga
    17581 ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt atttcttaag
    17641 tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg gcatcatgct
    17701 attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga aaaggcaatg
    17761 ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat ggggctattg
    17821 atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc gaaaatactt
    17881 gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa aaaaaccact
    17941 catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga agcgatatta
    18001 gggtatccgc atatcaggct tgcaattcaa gcagctaagg agctagataa tgattcatat
    18061 gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga cacatgcgta
    18121 ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca tgctgctagt
    18181 caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca ggaaaaccaa
    18241 atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct gaagatagag
    18301 gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta aataagacaa
    18361 aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag caaggctatc
    18421 ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa atcaattatt
    18481 tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa ggattcgctg
    18541 tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga ggttcatttg
    18601 gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga gattcttatg
    18661 ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt cttgcattag
    18721 ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa agacaaaaga
    18781 aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa aagaaagcac
    18841 agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa gaagaaactc
    18901 ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag aaagttctgc
    18961 aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt caggaagtag
    19021 gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc agatgctgtg
    19081 attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca caacgacagt
    19141 atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg caagccgatt
    19201 ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt gtgacaaaag
    19261 ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta tcggcaataa
    19321 ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat cgctcaaggc
    19381 tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca tctgcgataa
    19441 taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc ttattacttg
    19501 atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt tttgtaataa
    19561 taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt tttgtttgct
    19621 ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta tgtaagcgga
    19681 accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa ttgaaatact
    19741 tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa gtgagttcaa
    19801 tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt tgagcagtaa
    19861 aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc tggaaaagga
    19921 aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt tgaatcggcg
    19981 cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga tccatctaaa
    20041 caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata tgagacggtt
    20101 acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc aaggactggt
    20161 ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca atcagaatct
    20221 gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc aaaaaatctt
    20281 tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt ccaataagtt
    20341 gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat tgatctggat
    20401 ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata cagaaataaa
    20461 agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat tggacaatgt
    20521 cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg ctagagatat
    20581 ctccaattca atcgagtggg tacaagctca attactaacg atccaattga tccaatttgt
    20641 agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg aaaatctcta
    20701 taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca tgaatccatt
    20761 gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc attttgttcc
    20821 tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt ttcttgtcat
    20881 ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt ataaatcagt
    20941 cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc tgaaagaaaa
    21001 gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat tagtagataa
    21061 attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata agccgctttt
    21121 agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact ttttgaaaag
    21181 aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt tattcactca
    21241 aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct atggcaagtt
    21301 agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc agcctatgtt
    21361 aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta cttgggagga
    21421 atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag agcgctatgt
    21481 cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg gcaaacacat
    21541 ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca gcgtagctac
    21601 gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga ttattttttg
    21661 tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa ttgaattgat
    21721 caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag atttcaaaca
    21781 actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa agatgaacaa
    21841 attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag cacagatgtc
    21901 tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt gtgatatttc
    21961 ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc gttcgcagtt
    22021 cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata ttgtttcaaa
    22081 aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta tctaaataca
    22141 aaaagaaaag agggtaaaaa catgggcgtt atcaatttta tcatcgaaaa tattttgact
    22201 caggcatcga tcaccattgc attgattgct atgttgggtc tactactgca gaagaaatca
    22261 gcaggacaag tcatctctgg cacgttaaag acactcctag gatttcaagt gttgagtgct
    22321 ggttcgagca tcatcgttgg aagtttaact tatttcggga aaatctttac tgaaggattt
    22381 catatgcaag ggatcatccc ttctattgaa tctatcaatg gtcaagcaat gaatgatttg
    22441 ggtttaggta gagatattgc actgacgttc ttagccattt ttgtattcaa tattattttg
    22501 gctcgtttca caaaatggaa gtacattttt ttaactggtc aagcaatttt atggatggca
    22561 acgatgacga cagtctttgg ctattttgcc ggtctgcgag ggatcgtatt gatcttggtc
    22621 ggtggtttta tcggagcttg tttcgccatt gctatgccgg cagtagcgca accgatcatt
    22681 cgtaaaatca ctggatcaaa tgatattgct ttaggtcatt tttgtacgat cgggtactta
    22741 tttgaagccg gtgttgctaa gctttttggt gagaaaggcg aaaataaaaa gtcgatcgaa
    22801 gatatcaagt tgccgacaca tttcgaattt ctgcaagata cttatttgtc tgtcatggtc
    22861 gtgatggtcc cgctatatat catcactgtt ttatttgcag gcgaaccatt cgcttctgaa
    22921 ttatcaggag atcaaaacta tatcatgttt gcctttttac aagccatcca attcgtagtg
    22981 ggtgtgtatg tattgctagc tggtgtacgt ctactgttag gtgaaatcgt gccggcattt
    23041 cgagggatag ctatgaaatt agttcctgat gcgatccctg ccttagattg tccagttttc
    23101 tttccttata gcccaaatgc tgtcatttta ggtttcatta cgacaacgat tggtacgatt
    23161 attgccatgt ttactttgcc catgttcggc ttagcgatga ttttacctgg tatgctaacc
    23221 aatttctttg ccggagggac agcagggata tttggcaacg cagttggcgg acgccgagga
    23281 gcgatcattg gcggtattgc tcatggtttc tttattacat tattgcccgc tttgctagta
    23341 acgatcttta attcgatggg attcaacaat gctacagcaa ccgatgtaga tacagtagcc
    23401 gcagcactct tatatgcctg gatcttaagc ccagttttga aagcatttta attgaaagta
    23461 gggaaaatga tgtttggatt ttttaagaaa aaagaaaaag aaccgaaaaa agttctggca
    23521 aaagaagaaa aagccctatc gccagaagcg accgaaaaga ttcaatcaga gattacacaa
    23581 ttgaaacaag agatttccac tacccaagat aagcataaat tagcgaaact ttatgaacaa
    23641 gttggactta aattttctga attgtatgtg aacgatcaag ccatccaata tttagaaaag
    23701 agtttggaaa ataaacagac gatcggagat gggtataaaa aactgatgag cctctataac
    23761 cagaagcgtg cagatgctgc tcgtgctggt gatgatcaag ggatcgatta ctacatgggc
    23821 aaaatggatg agatgcgaca aattgcaaaa caagtaacga tcaaaggaaa caaataaaaa
    23881 gataaggtgg caataaaaga tgtatacaac attgaaagaa gtaacaaaaa cagcagaaga
    23941 gttgaatttt acagttggag cttttaatac gcataaccta gaaatgcttc ctgctatgct
    24001 aggcgcagca aaagaaatgg gttcgccgat cattattcaa acaagtgtcg ataccgctaa
    24061 atatatcggc atgcatgtgt tagtcaatgt aatgacagct attgcagaag aagaaatggt
    24121 tgatgcagtc ttgcacttag accacgcccg agactttgat gatatcaaag aagcaatcga
    24181 cagcggatat acttctgtca tgtatgatgg gtcccatctg cctttcaaag aaaatatcct
    24241 gaagacaaaa gcagtagtag aatatgccca tgcgcacggt gtttccgtag aaggagaact
    24301 tggaacaatc ggcggtacag aagaaggaat ccatgtcgct gaaaatgaca aggtatatac
    24361 aagaccggaa gacgcagtga aatttgtaga agctacaggc gtggatgcat tagctattgc
    24421 tatcggtacg aatcatggac aattcaaatc aaaaacagaa gtaaacattc ccttgctgaa
    24481 agaaatcgat gcagtagttg atgtccccct agttatccat ggaggaacag gtgtgaaaga
    24541 agaagattat cctgaattga tcaataacgg gatccgcaaa ttcaatgttg gaacggagct
    24601 tttagtcaac tggacgaaag tagctaaaga aacctttagt gaaacagaaa tcaacaagtc
    24661 tcttcgccat aatgtgattc cagcaaatga agcagtaaaa gaaattgtca agcataagat
    24721 tggactgttt atgaacttgg aaagtccaat taacttagga aataaatgag atgaaaaagt
    24781 atttcattct tctggctggt agtcctggta caggaaaaac atatcttatg aataaactac
    24841 gggaacgatt tccagatatg tatgccatca cgctcgatga aatcaaggaa tactatgcag
    24901 aatccatcgg gtttaacaat ttggaagaac gggcggaaca agaaaggacg aaagtttatc
    24961 ctttctatta caaagcactt gatttgtata tggaagcagg aaagaaagtg attgtgtccg
    25021 agtatccttt tagtgataag caaaaagcac gactccaaga attagcagat agatacggat
    25081 atcaggtgat cacgattcgc ctaacagcag actttgaagt gttatggcaa cgtcggtaca
    25141 aacgtgatcg tgaatcagat cgtcatttga gctatatcat ggatcattat cactatggtg
    25201 acgtattgga agatcgaacg aaagggacca atcatattac gaaagaagca ttcaaaaaga
    25261 tcatcgccga ccgaaaatat gacaaattcc agttaggaac actatatgaa tttgatgtaa
    25321 cagactatac tcaagtggat tacactccac tattggataa attagaaaaa gaaatcaatg
    25381 acaataagta acaaagaaat ggcttaatat cgaaaaattg gcttttgaac gctgtttacc
    25441 gagaggtcgt gagcctgtcg ttcagctccg agtcacaagg agcaatttca aaaaaacagc
    25501 ttctcatgtt tgatgaatat tgtgacttgt gaccgaggag ttggtaggag aacaccgttt
    25561 agtggggagg ttgtgaaaaa gctgtcctgc ctcaattaat aagaacagcc agacaagaat
    25621 agctcgtcat atttttcgtt tggtggggct tattaccgca gatgcaagct tttgaacgcc
    25681 atttattcac agccccagag aagtcgaatg tcttttgttc tcgagttttt taacattctt
    25741 ttatttattg cagatttcat tatatttatt tgccgtaatt aaaccagtct tctatacttg
    25801 attacatagt catacatttg ctaaaaaaga agggaagaga atgaacaaga agttggctca
    25861 atacaaaaga ctactcgcac tttctcaagc tggtctatat tatggaaaag atgtatttga
    25921 taaagagcgt tttgaggaga tccggacgat ttcattggaa ctgatcagtg aaataggaaa
    25981 agaaactttc gaagaaataa aagcgctcac aacgattgga gaaggacatc caacaccaaa
    26041 agtagatgtt cgtgcctata tcaaaaaaga tggcaaggtc ctactaatcg aagataaacg
    26101 gacgaaagaa tggtcacttc ctggtggatt tgctgaaata ggattgtctc ctgaagaaaa
    26161 tgtgcgtaaa gaagtctatg aagaaacagg attaacagta gaaactactc agttgcgagc
    26221 agtttttgat acgaataaac aaaaagatat tccacagctt tttcagtatt ataaacttgt
    26281 ttttgcttgt gcgatccacg gtgaagaggc caagtttatt gagaataatg aaacttccaa
    26341 catgggattt tttagtatag aagagttgcc aaagctatca gaaaagcgaa caacgaagaa
    26401 acagttactt attttagaaa acaaaaacca gatctattgt gattgagctt tttcgtaaaa
    26461 gaagaaaaga atatacttta ctagttatta aaaaagaact tgattttttt attgaaaata
    26521 gttgcatttg caacaaaaag aggtgaacta gtcttacaaa caaaataaag gtgggatgaa
    26581 atggctgaaa tccttcgaga catcggaatg attgcccgag cgctagattc tattagcaat
    26641 atcgaattca aagaagtcga tttaacaaga ggacagtatc tttatttagt acgtatttgc
    26701 gaaaatcctg gaatcatcca agaaaaacta gcagaaatga tcaaagtaga tcgtacaacg
    26761 actgcccgag caatcaagaa attagaatcc aatgggatga tcgaacgttt ggaagataaa
    26821 gaaaataaaa aaatcaagaa actctatccg actaaaaaag gagcagaaat ctatcctttc
    26881 atcattcgag aaaacaacta ctccaatgca gtcgcattaa atggtctttc tgacgaagaa
    26941 gcaaaacaat tggaatatct tttaaataaa gtatgcaaga atgttagcga agattggaat
    27001 tttgtcaaaa aaggcaataa aaggatctat tgaaaggaag aagccgtgaa taaatacgaa
    27061 attcgaaaaa tgacgccttc agatgtagac ttactacaac aaattagtat cgaaacattt
    27121 acagatacat ttggaaaaga taattcaccg gagcatttag ctgattactt gacacaagct
    27181 tatgcactag aaaaacttac agaagagcta aaagataaag aaaccgattt tttcctagtg
    27241 agtatcgatc aagagcctgc gggttacctg aaactaaata caggaacgag tcaatctgag
    27301 gaaatggctg atcatttttt agaaatcgag cgaatctatg tacgaaaaaa atttaaacat
    27361 caaggtatcg gtacattttt actagataca gctattgaaa aagcaaaaga aaagcaaaaa
    27421 acagccattt ggctcggtgt ttgggaacac aacgatgttg cgctcgcttt ttatcgtaaa
    27481 aaaggattta tcaaatacag cgaacatgtt ttttatatgg gagatgatcc ccaaacagac
    27541 tatctattca tcaagcaatt attctaatca agaggtgtga acagatgaaa aaagtaatag
    27601 tggataccaa tttctgggaa gtatttcctg aagcaaccat cgaaatctta tccgtaaatg
    27661 gtatcgataa ccacgtaaca aaagaaagtg aagaaacgta tcatcaatta ttgaatgagg
    27721 cagcaaaaga agcaagaaat taccttacag aagaaacatt cagccaaaac gaagtcattg
    27781 ctcagtggcg tcaggcattt accacattca agactaaaaa aggtgcacgt tcttctattg
    27841 aagctttact gaaacgtgca aatcaagaac gggaattttt cccaatcaat ccgcttgtag
    27901 atatctataa tagtatttcc ttgtcttttg gcgtaccttg cggcggagaa gatatcgaaa
    27961 agatcgcagg aaatctgcat ttaggaaaag cccaaggtgg cgaatcattc ctgcctttag
    28021 gtgcagattc cgatgctccg gcacttccgg aagaaatcat ctactatgat gaacaaggag
    28081 cgatctgtcg ctgtctcaac tggcgtgaag cccaaagaac gatgctgacg gaagaaacac
    28141 aagcagcggt gctggtcatt gaatccatca atgctgaaca agcggcacgt ggcgcacagg
    28201 caatcgaagc gttgaaggaa aaaatcgatc aaacatttgg cgtaactagt gttagacaca
    28261 cactaaccaa agatgaatca gaagcagaat tataaaagaa tcgaaataa
//
LOCUS       DAXWLG010000016        28277 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXWLG010000016 DAXWLG010000000
VERSION     DAXWLG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.016"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(211..399)
                     /gene="rpmB"
                     /locus_tag="ACGE4T_000555"
     CDS             complement(211..399)
                     /gene="rpmB"
                     /locus_tag="ACGE4T_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFF8385863.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            669..1031
                     /locus_tag="ACGE4T_000556"
     CDS             669..1031
                     /locus_tag="ACGE4T_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8385864.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1083..2765
                     /locus_tag="ACGE4T_000557"
     CDS             1083..2765
                     /locus_tag="ACGE4T_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFF8385865.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2882..4918
                     /gene="recG"
                     /locus_tag="ACGE4T_000558"
     CDS             2882..4918
                     /gene="recG"
                     /locus_tag="ACGE4T_000558"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFF8385866.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALTGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4967..5968
                     /gene="plsX"
                     /locus_tag="ACGE4T_000559"
     CDS             4967..5968
                     /gene="plsX"
                     /locus_tag="ACGE4T_000559"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFF8385867.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6090..6332
                     /gene="acpP"
                     /locus_tag="ACGE4T_000560"
     CDS             6090..6332
                     /gene="acpP"
                     /locus_tag="ACGE4T_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8385868.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6600..7604
                     /locus_tag="ACGE4T_000561"
     CDS             6600..7604
                     /locus_tag="ACGE4T_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385869.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7605..8549
                     /locus_tag="ACGE4T_000562"
     CDS             7605..8549
                     /locus_tag="ACGE4T_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385870.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8549..9511
                     /gene="opp4B"
                     /locus_tag="ACGE4T_000563"
     CDS             8549..9511
                     /gene="opp4B"
                     /locus_tag="ACGE4T_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8385871.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9538..10452
                     /locus_tag="ACGE4T_000564"
     CDS             9538..10452
                     /locus_tag="ACGE4T_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8385872.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10478..12259
                     /locus_tag="ACGE4T_000565"
     CDS             10478..12259
                     /locus_tag="ACGE4T_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8385873.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12607..13293
                     /gene="rnc"
                     /locus_tag="ACGE4T_000566"
     CDS             12607..13293
                     /gene="rnc"
                     /locus_tag="ACGE4T_000566"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFF8385874.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13313..16894
                     /gene="smc"
                     /locus_tag="ACGE4T_000567"
     CDS             13313..16894
                     /gene="smc"
                     /locus_tag="ACGE4T_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFF8385875.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16903..17712
                     /locus_tag="ACGE4T_000568"
     CDS             16903..17712
                     /locus_tag="ACGE4T_000568"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8385876.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17724..18722
                     /gene="ftsY"
                     /locus_tag="ACGE4T_000569"
     CDS             17724..18722
                     /gene="ftsY"
                     /locus_tag="ACGE4T_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFF8385877.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18909..21176
                     /gene="gshAB"
                     /locus_tag="ACGE4T_000570"
     CDS             18909..21176
                     /gene="gshAB"
                     /locus_tag="ACGE4T_000570"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFF8385878.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWKEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21264..21716)
                     /locus_tag="ACGE4T_000571"
     CDS             complement(21264..21716)
                     /locus_tag="ACGE4T_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFF8385879.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            21866..22816
                     /locus_tag="ACGE4T_000572"
     CDS             21866..22816
                     /locus_tag="ACGE4T_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8385880.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22809..23768
                     /locus_tag="ACGE4T_000573"
     CDS             22809..23768
                     /locus_tag="ACGE4T_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8385881.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23765..24520
                     /locus_tag="ACGE4T_000574"
     CDS             23765..24520
                     /locus_tag="ACGE4T_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385882.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24543..25496
                     /locus_tag="ACGE4T_000575"
     CDS             24543..25496
                     /locus_tag="ACGE4T_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8385883.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(26140..26436)
                     /locus_tag="ACGE4T_000576"
     CDS             complement(26140..26436)
                     /locus_tag="ACGE4T_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFF8385884.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26792..27148
                     /locus_tag="ACGE4T_000577"
     CDS             26792..27148
                     /locus_tag="ACGE4T_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8385885.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            27158..28057
                     /locus_tag="ACGE4T_000578"
     CDS             27158..28057
                     /locus_tag="ACGE4T_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8385886.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         9352 a         4976 c         6115 g         7834 t
ORIGIN      
        1 gttgtattca gtttatcata tccttgtttt tctctgctta aaaagagtct aattttcaca
       61 aaaaaataaa atttagaata aataacttca taatttgttc gtataacaaa aataaacgca
      121 aaaaaagggt aggaatcaaa agtcccaccc ttaaaaattt ttattaaata aaaaaccgtt
      181 cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc ctgatttcaa
      241 agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc gaactttttg
      301 taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt tgtttccact
      361 ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct cctttgcctt
      421 gatttggagc aatagaataa ttcagctcat accagactaa catatcatat agaatggaaa
      481 tttgcaagta cattttcata gatacaattg acaattgaga agaataattg taaattaaac
      541 aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc cttttatttc
      601 aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc cgttaaggag
      661 gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt accaatgatg
      721 taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta ggaatggcca
      781 gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat tactcaagag
      841 gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg attatcagct
      901 atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa tacaatctag
      961 aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt gttcgtgtac
     1021 tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg aggattatct
     1081 aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag gcaggtgcga
     1141 atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct gtgccagatg
     1201 gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca gtagtagatt
     1261 caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg ctgatgggtg
     1321 cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct aagcaaattc
     1381 cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat ggagttgaaa
     1441 ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta gctagagaag
     1501 cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa gtaatgacag
     1561 ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt ccagtcctaa
     1621 aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat gagggctttt
     1681 taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct ccagcagaaa
     1741 tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg gcgacagaag
     1801 atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga cctacagtcg
     1861 atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga gattctttac
     1921 ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac ccaggagaag
     1981 tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac aacatgcgct
     2041 tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg aaaaaagaaa
     2101 caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc caagagctgt
     2161 ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat ccaagtacgg
     2221 aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc ttgccaaaca
     2281 ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc ccagtagcag
     2341 ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc aatgatcaag
     2401 cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt gtgagcggtc
     2461 aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa gaaggagact
     2521 ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca gcaacagcgt
     2581 taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc ttggcaggtg
     2641 atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa aaacatcctg
     2701 atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta ttttctgctg
     2761 aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa ggccttcggt
     2821 tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg aggaaaaatt
     2881 tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa aaagagcagc
     2941 tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt atcctttccg
     3001 ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa aggttacttt
     3061 gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa aaagccgatt
     3121 gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta atcaacctta
     3181 tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat gggatgcaaa
     3241 gcgtaaagca ttgaccggga tgaagatttt aggttctcaa acaatggatg acttttcgcc
     3301 gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa tcaaaaaagg
     3361 gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg tggagaaata
     3421 tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag accacgaaga
     3481 aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc aaatgcgtct
     3541 tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc tttatgatgt
     3601 ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg cacaaaaacg
     3661 tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc gacttttgca
     3721 gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg ctaccgttac
     3781 agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac aacatatgga
     3841 aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta cggggtcaac
     3901 gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa ttaacatcgt
     3961 gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag gtctagttat
     4021 tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg aaaaaggctg
     4081 gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag ctattacagc
     4141 atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta tcccgatcga
     4201 aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga aaaaagaact
     4261 ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag aagcattaga
     4321 tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac caaaatatga
     4381 agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca tggaagcctt
     4441 caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag gggtaaacgt
     4501 gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg cacagctgca
     4561 tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct tagttgctaa
     4621 tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca ccaatggttt
     4681 tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg gcttccgtca
     4741 atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg ttttggaggt
     4801 agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc ttcctgagta
     4861 tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt tcgattagca
     4921 gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga aaattgccgt
     4981 agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg ctttagcgca
     5041 gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa tcaagaaata
     5101 cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca atagtgatga
     5161 tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg ctgcacaagc
     5221 agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg cgctactagc
     5281 agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct tgatgtctac
     5341 cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg gcgctaacgc
     5401 agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt atgcacgtaa
     5461 agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag aagcaacaaa
     5521 aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa gcttgaattt
     5581 tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag tggtcacaga
     5641 tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga atatgatgaa
     5701 tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg ctatgctttt
     5761 aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg gcggtgccgt
     5821 tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc ccgatgctgt
     5881 tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg gacaattagt
     5941 cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc cgttccattg
     6001 aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt aggtaattga
     6061 caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt aacaaagtag
     6121 cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat gaattgaaca
     6181 tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta gagcttgaag
     6241 atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca gtcggtggcg
     6301 cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag aaggataaaa
     6361 agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa aagtcatttt
     6421 tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt ttctatataa
     6481 ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg ctttattctg
     6541 tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg ggtattcgat
     6601 tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga ctcaaagatg
     6661 actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag attttagcaa
     6721 tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg ggattacttg
     6781 atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat ttaatcgacc
     6841 tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt ttccaagatc
     6901 cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa ggactttcct
     6961 atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg ctagaacaag
     7021 tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta tcaggcggta
     7081 tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc gtcattgcag
     7141 atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat ttactgaaag
     7201 accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta ggagtcgtag
     7261 cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa gtagcaggtg
     7321 taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg aactctatac
     7381 cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt ccttctctga
     7441 aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata ccagcaagcg
     7501 atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta cgttgctcct
     7561 gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca gaattaatca
     7621 caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat cgagtaacgg
     7681 atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag acatatggtt
     7741 tagtgggaga atctggctct ggaaaatcta ctacgggaaa agccgttgtg ggattagaaa
     7801 aggtgacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga agcaaccgca
     7861 aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg tccagtttga
     7921 atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt gaacggttaa
     7981 gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg atgccggaag
     8041 atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt ctaggagtag
     8101 cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta tctgctctgg
     8161 atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa gaatacggcc
     8221 tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc gacaatatcg
     8281 caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt tataatgacc
     8341 cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat gtagaaaata
     8401 gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa aaccaaaaag
     8461 aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact acacacaaag
     8521 tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac gtgttttatt
     8581 gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc aagcgatgcc
     8641 gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga tcgaacagat
     8701 gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt ggatcggaaa
     8761 tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg ctgacttgat
     8821 cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc ttacttattt
     8881 gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct tggataagat
     8941 tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg gcttgatcat
     9001 gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac aaacactagg
     9061 tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc tgttaccatc
     9121 aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa gtgaagtaat
     9181 cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac cgacgaataa
     9241 agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc aaatggggta
     9301 tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg gttatccggg
     9361 agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga tcacatcctt
     9421 agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata tcatcatgag
     9481 tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa tggatcgatg
     9541 agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat acgaatgatt
     9601 gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct agtggtttta
     9661 ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat gtatgttagt
     9721 atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa gttcattctg
     9781 ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc tagaaactct
     9841 gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg attagggatc
     9901 atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt tgattttatc
     9961 atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc cagatacaat
    10021 gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc ccgattattc
    10081 cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa aacaatggga
    10141 accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc attaatcatt
    10201 actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt gactttctta
    10261 ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta tgcaagtaac
    10321 ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct tattttagtt
    10381 ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga tgcgcgccaa
    10441 cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga tttttggatt
    10501 tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta cagatacctc
    10561 tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc caattgcaac
    10621 aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg caacggacag
    10681 tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg cagcttttat
    10741 ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca cagacgacgg
    10801 agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag taaaagacaa
    10861 cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc cttacgaaat
    10921 cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta acatcgtagg
    10981 tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa aaatagacga
    11041 caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat cgggtggtgg
    11101 tatttggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca aagatatgga
    11161 atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca tgagtaatat
    11221 cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg gtacgcctaa
    11281 attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag cattgaactc
    11341 taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct ataaagaaat
    11401 tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag gattcaagtt
    11461 aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa aaatggctga
    11521 taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag gtgagcgttt
    11581 ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc agtcatacca
    11641 tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac tattagatga
    11701 tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg gaaataaact
    11761 aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg cagattacta
    11821 tatccaacag tggaaagaaa tcggtttgga tgtccaatac acaacaggtc gtctgatcga
    11881 attcaattct ttctatgata aattagaaaa tgatgatcct gaaattgatg tttaccaagc
    11941 tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa atgctgcgtt
    12001 caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata tcagttcaag
    12061 caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac aatatgcatt
    12121 tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag tcaacgaccg
    12181 tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg tttctgttac
    12241 agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc ctttttcggt
    12301 tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga tttcatgact
    12361 tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt tatgcaaaaa
    12421 tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg aatttttcac
    12481 aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc gaaccatttc
    12541 aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat aaaaggaatg
    12601 tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat cgtttttcac
    12661 gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga gcatcgcaat
    12721 ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt agaactgatg
    12781 gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt gacaaaaaca
    12841 cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg tcactttgat
    12901 caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg accagcattg
    12961 ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg attcgacacg
    13021 acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc tttttcacat
    13081 gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga tgtatcaatc
    13141 gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg gatcgaagtt
    13201 tatgtagatg atcaattgat tggcactgga caaggaaaat cgaaaaaatt agcagaacaa
    13261 gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag ctttgtattt
    13321 gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca ttgactttga
    13381 aaatagcgtc acagcagtgg ttggaccaaa tggcagtgga aaaagcaata ttacagaagc
    13441 tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa aaatgccgga
    13501 tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag tcacagtgat
    13561 tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg taacccgccg
    13621 ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca gattaaaaga
    13681 tatccaagaa ttgttcttag actctggatt aggaaaagaa tcgttctcaa tcatttctca
    13741 aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga tctttgaaga
    13801 agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga agctttttga
    13861 aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag aacagttaac
    13921 tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag aaacactgac
    13981 tcaaaccgat gtctctttga tggttgccga aatcaagaca gcaaaaaaag attgggacaa
    14041 caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg agtcgatcca
    14101 agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg atcgtctgat
    14161 cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga cagaaggaca
    14221 aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag aatatcaaac
    14281 ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag aaagcttgtt
    14341 gaaagaagta gcagaaaaag aaacagaaat ccaaaaagct gaagcaagtc tcataaaaac
    14401 acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttactggcag aactaagaga
    14461 tcaatatgtt gatttgatgc aagagcaagc agctgtagga aacgaactga agtatctaga
    14521 acgtcaatat gtacaagaaa cagcaaaaag taagcagacg ttagccaagc aatcggaagt
    14581 agaggcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa agcaggcgca
    14641 gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac aaaacgggaa
    14701 aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa tgaatcaagt
    14761 ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact attttggttt
    14821 ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta ttgtcggagc
    14881 agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaaa cagctttagg
    14941 tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag ccatcacgta
    15001 tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa tcaaaccaag
    15061 acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta tagggattgc
    15121 cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc tgctaggaac
    15181 gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga tccgctatca
    15241 atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca tgacaggtgg
    15301 agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat tgaaacagtt
    15361 gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa aagttcagga
    15421 gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta cacgaggcga
    15481 acaattacgt tttgaagaac aagaagcgac aaatcagctg caaaacatca taaatgaact
    15541 tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac tgcagcagtt
    15601 tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag atcttgaatc
    15661 gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg accagatgga
    15721 agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc ttgctgtctt
    15781 gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc aaaaagcaga
    15841 ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag cagacttttc
    15901 tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat ctgctcagcg
    15961 agaaacattg aaagccgagt tggcaaaagc caaagagcag cgagatagaa ggcaaaagga
    16021 gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag cacgactgtc
    16081 ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag ataatcattt
    16141 gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag actatcaaga
    16201 aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac agattgaaaa
    16261 actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg aaagacatac
    16321 attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt ttgaaacgat
    16381 ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag ctatccgtca
    16441 agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat tagtactgac
    16501 agaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc ctggcaaaaa
    16561 actgcaaagt ttaagtttgc tttctggagg agaacgagca cttacagcca tcgcgttgct
    16621 cttttctatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg tagaagcagc
    16681 gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc aagatgacac
    16741 acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg tactgtacgg
    16801 agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg aagatatcaa
    16861 tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa attagtagca
    16921 atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga gaataaacaa
    16981 gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg acgcccctta
    17041 gctgcgatgg tgcattatct gcaagaatta ggactggtag atgaaggtga ctacagcatc
    17101 actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga aaagaaagtg
    17161 atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga tctgccacta
    17221 gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa gcaatccttg
    17281 tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga tattacagat
    17341 gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga tcctaaaatc
    17401 aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacgag aagtatgctt
    17461 ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt ggcaaaagat
    17521 cttggtttga aacaagaaga aatcatggca atcggtgatg aagaaaatga tttaccgatg
    17581 atccaatacg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt caaagaagca
    17641 gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat ccaaaaattt
    17701 gtattagagt aggggggagt caaatgggat tttttgataa aatcaagcgt gcttttactg
    17761 gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt ttagaaaaga
    17821 cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga acagtcgatg
    17881 aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt tttgaaacag
    17941 cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg aaaaaatcgg
    18001 cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg gaaggaatca
    18061 atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg tttgtcggtg
    18121 ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc aaaaacgaag
    18181 gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc gaccagttag
    18241 ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcaggc ggcgatccag
    18301 cagctgtcgt attcgatgct ttggaaagag caaagataga gcaggcagat gttttactag
    18361 ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta gaaaaaataa
    18421 aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt gtactagatg
    18481 caacaactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca acagatgtga
    18541 ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta cttgctatcc
    18601 gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc gatgacttgg
    18661 agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta aaagaagaat
    18721 aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt tgaatgattg
    18781 tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta agcggttctc
    18841 aaacaatttc gttctttttc ttagactttt tgattataat tagattcata tgaattggag
    18901 gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc ccttttatcg
    18961 accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca ggaaatctag
    19021 caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat atccaaacag
    19081 attttagcga aacacaaaca gagatgatca ctcctgttac cgattctatt cccgaattat
    19141 ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa gaagagatga
    19201 tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagcgatc attattgcaa
    19261 aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaagaa tacgggaagc
    19321 gaaaacagat ggtaagcggt atccatttca attttgaatt tggcgatgag ttgctaagaa
    19381 cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca gaactttatt
    19441 tgaaaacggc gagaaacttt atgcgctatc gctggatgat cacttattta ttcggtgctt
    19501 caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa cctgttcgca
    19561 gtattcgtaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt tcctatgctt
    19621 ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa ctttcggagg
    19681 agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca gatttagcaa
    19741 caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat gcaagattgg
    19801 gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg ttatggaaag
    19861 aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat aacaaagtag
    19921 cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa attcttgaag
    19981 ggatgaagca aatgctggtt gaattggatt ggttggattc tctttattta gtagaggaag
    20041 cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat caagaagcac
    20101 agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat aaagaaagcc
    20161 atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact cagatattta
    20221 tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg gatcaatttt
    20281 tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg acaagtaagg
    20341 acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa gtcttaaaag
    20401 aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa gcggtaaaag
    20461 cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc aattacgggt
    20521 taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca ggtttagcca
    20581 ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga acggaatatc
    20641 gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct gctaatgtta
    20701 ttggtgacgg tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt gaccctttgc
    20761 gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta gaacaattga
    20821 tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa attgtttatc
    20881 taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact gatgaatttt
    20941 ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct aagattagtg
    21001 gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa aaagcatatg
    21061 ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatccc tttgcaggaa
    21121 aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta ttttaaaaga
    21181 aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt ttttgcatgt
    21241 aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt tttctttttt
    21301 ggtttttctt tttcaactgg cgaagagttt tcttctgggt attttttttc gatatcaatc
    21361 acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc aggatttgca
    21421 gtgacgtaat cgttgactac agtaaatcgg ctattgtttt tcgttgctag ctggatatat
    21481 tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc tggatgtttt
    21541 ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc aacagtcata
    21601 ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc agggttcact
    21661 aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg tgccataaaa
    21721 aataccctcc ttcttctctt tgtctttctt acgattatag catattccca ataataccta
    21781 aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag aatgaaaatc
    21841 attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat tattagttat
    21901 tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag aactgctcca
    21961 gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa cgatttgttt
    22021 gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt tgacccaaaa
    22081 taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag gtattttagt
    22141 ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag cattttgctt
    22201 tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttccccc agaaaaatca
    22261 attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag ttgctacttt
    22321 ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag gaaattttgc
    22381 gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt tgctcttggt
    22441 ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg cagtaaattt
    22501 gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat tggctagtgc
    22561 agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc cgaatcttgt
    22621 ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt tagcaggtag
    22681 tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt atgaaatacc
    22741 agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc tgataagggg
    22801 gcgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt cttgttttat
    22861 ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt gcatgggcat
    22921 tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc agtgccacaa
    22981 tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg ggacttgagt
    23041 cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg acgatgcttc
    23101 agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg ctgagtctat
    23161 tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta ctgatgtcag
    23221 ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc atcatggatc
    23281 cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat atccagagtc
    23341 agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta tttcgatatc
    23401 gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc gggatagaca
    23461 cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg acatcaacgg
    23521 ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt tatcgtgtct
    23581 tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct atgctgtttc
    23641 tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca gtgagtgtcg
    23701 tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa aggaagagta
    23761 tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag tggttaaaga
    23821 tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa atggtgctgg
    23881 aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag gtgaaatcta
    23941 tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa aattatcgat
    24001 cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag tagaatttgg
    24061 acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg ttcaggaatc
    24121 actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc tttcaggcgg
    24181 tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt atattttact
    24241 ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc agatcttaaa
    24301 aaaattagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata tcaactttgc
    24361 tgcaagttat gcagacgaaa ttattgcaat gaaagatggg cagttattca cgcaaggaac
    24421 gacccaagaa atgataaaaa aagatgtact ggacgaattg tacgaaatgg acatacgaat
    24481 ttgtgagatt gaaggtcgca gattctgtct ttatttctaa aactaacatt aggagagaag
    24541 agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta gcagcatgtg
    24601 gaaatagtga caacaccagt caggcagata caaagaaaag cagtacgcaa gctgaaacgg
    24661 cattaacgat aaaagacagt aatggcgatc aaatcgaggt gccaaagaat cctgaaaaag
    24721 tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt ggagataagg
    24781 tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa aaagtggaat
    24841 cagcaggtgg gatcaaagaa ccagacttag aaaaaatcaa tcagcttcag ccagacctga
    24901 tcatcatttc tggccggcaa agagattttc aaaaagatct aagtcaaatt gctccgacca
    24961 ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat gtcactgctt
    25021 taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa ttatctagtg
    25081 caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta gtcacacttg
    25141 tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc atccatgatt
    25201 tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt caaagtgttt
    25261 cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac agaacaaaag
    25321 ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta gcacaaacga
    25381 atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat ttaagcgggg
    25441 gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt aaatagcata
    25501 aataacagag gattaaactc tcatactatg taacaataat gtttcttaca ttcttaaata
    25561 gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta ttgatgattt
    25621 tacggatggt ttttatttat ttgggaaatc ttttttcaac gtgaattcat tctctacatc
    25681 atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt ttttcagcat
    25741 caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat aaaacttaaa
    25801 tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc tgctgcttct
    25861 cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca gcgttcttat
    25921 tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac gatgttccat ctgctgcttc
    25981 tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta
    26041 ttacttaaag caaatcgtag ttgtcacaat ctcttctaaa cgatgttcca tcagctgacc
    26101 ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg tctcagcaag
    26161 cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct tactccaccg
    26221 ttttaataat tgatagaaat aactctcttc tgctttgatt ttatactcgc ttaatatcaa
    26281 tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg ctttggtttt
    26341 atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat tttgtgtttc
    26401 ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa agaagtcttt
    26461 ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa aaagtaattc
    26521 tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt atttaagaaa
    26581 ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc aagaaactga
    26641 acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa acgaatgact
    26701 tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc tgatgaataa
    26761 agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa aagaagcaca
    26821 agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata tcgaaaacgg
    26881 tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gacacggcat ttcgattgat
    26941 cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg ctgatacacc
    27001 agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc catccacact
    27061 tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct tgagaaaagg
    27121 caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg cgatcatcag
    27181 cgacctacat gtggacatca agaaattcgg cgaatcagag ctgatgcagc tttggcaagt
    27241 tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta ataaagtaga
    27301 ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga catttcattt
    27361 cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt ttccagaccc
    27421 acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt tagggatgaa
    27481 tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg tacgaatgaa
    27541 gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg aggtcaatgc
    27601 agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa aacttgaggt
    27661 gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat ctgctcctta
    27721 tgagcgctgg aacaaattaa atgcattttt aggatcagaa tcattcggag aattacttga
    27781 ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct ttgaagataa
    27841 atccatcggt cacacgattt acagctgcag accttttggt tattattatg aatggcagct
    27901 gacccgagat tttgtcttga aagaacagct gttagatagc tatgaaccca tgaaattacg
    27961 tgtcctcttg cgtgcccatc atccggcctt tcaagcctat caaacacaac atttgcaaga
    28021 ggaatttgaa caggcgatga caatcgttcc gtattgagac agcattagta atgtgtaaaa
    28081 gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta tcgtggatat
    28141 gctgcttttt ttgtggaaaa taatcagcga atgtttattt gatcggatga tttgtaactc
    28201 gcagagtact agatgcaagc gtttattctt tttttgaacg ataatcacaa aaaaaaatat
    28261 aatatacatt ttttatt
//
LOCUS       DAXWLG010000017        28191 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXWLG010000017 DAXWLG010000000
VERSION     DAXWLG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.017"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(82..777)
                     /locus_tag="ACGE4T_000579"
     CDS             complement(82..777)
                     /locus_tag="ACGE4T_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFF8385887.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE4T_000580"
     CDS             complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE4T_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFF8385888.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1976..2167)
                     /locus_tag="ACGE4T_000581"
     CDS             complement(1976..2167)
                     /locus_tag="ACGE4T_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFF8385889.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2188..3078)
                     /locus_tag="ACGE4T_000582"
     CDS             complement(2188..3078)
                     /locus_tag="ACGE4T_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFF8385890.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3065..3832)
                     /locus_tag="ACGE4T_000583"
     CDS             complement(3065..3832)
                     /locus_tag="ACGE4T_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8385891.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE4T_000584"
     CDS             complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE4T_000584"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFF8385892.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE4T_000585"
     CDS             complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE4T_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFF8385893.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE4T_000586"
     CDS             complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE4T_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFF8385894.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLNTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8403..8786
                     /locus_tag="ACGE4T_000587"
     CDS             8403..8786
                     /locus_tag="ACGE4T_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385895.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE4T_000588"
     CDS             complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE4T_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFF8385896.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9730..10548)
                     /locus_tag="ACGE4T_000589"
     CDS             complement(9730..10548)
                     /locus_tag="ACGE4T_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFF8385897.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE4T_000590"
     CDS             complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE4T_000590"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFF8385898.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE4T_000591"
     CDS             complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE4T_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFF8385899.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE4T_000592"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE4T_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFF8385900.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13208..14338
                     /gene="dnaN"
                     /locus_tag="ACGE4T_000593"
     CDS             13208..14338
                     /gene="dnaN"
                     /locus_tag="ACGE4T_000593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFF8385901.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14567..14809
                     /gene="yaaA"
                     /locus_tag="ACGE4T_000594"
     CDS             14567..14809
                     /gene="yaaA"
                     /locus_tag="ACGE4T_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFF8385902.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14796..15920
                     /gene="recF"
                     /locus_tag="ACGE4T_000595"
     CDS             14796..15920
                     /gene="recF"
                     /locus_tag="ACGE4T_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFF8385903.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15917..17863
                     /gene="gyrB"
                     /locus_tag="ACGE4T_000596"
     CDS             15917..17863
                     /gene="gyrB"
                     /locus_tag="ACGE4T_000596"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFF8385904.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17883..20354
                     /gene="gyrA"
                     /locus_tag="ACGE4T_000597"
     CDS             17883..20354
                     /gene="gyrA"
                     /locus_tag="ACGE4T_000597"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFF8385905.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20590..20889
                     /gene="rpsF"
                     /locus_tag="ACGE4T_000598"
     CDS             20590..20889
                     /gene="rpsF"
                     /locus_tag="ACGE4T_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFF8385906.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20939..21451
                     /gene="ssb"
                     /locus_tag="ACGE4T_000599"
     CDS             20939..21451
                     /gene="ssb"
                     /locus_tag="ACGE4T_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8385907.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21477..21713
                     /gene="rpsR"
                     /locus_tag="ACGE4T_000600"
     CDS             21477..21713
                     /gene="rpsR"
                     /locus_tag="ACGE4T_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFF8385908.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21856..23829
                     /locus_tag="ACGE4T_000601"
     CDS             21856..23829
                     /locus_tag="ACGE4T_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFF8385909.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23835..24287
                     /gene="rplI"
                     /locus_tag="ACGE4T_000602"
     CDS             23835..24287
                     /gene="rplI"
                     /locus_tag="ACGE4T_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFF8385910.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24561..25928
                     /gene="dnaB"
                     /locus_tag="ACGE4T_000603"
     CDS             24561..25928
                     /gene="dnaB"
                     /locus_tag="ACGE4T_000603"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFF8385911.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26708..28000
                     /locus_tag="ACGE4T_000604"
     CDS             26708..28000
                     /locus_tag="ACGE4T_000604"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFF8385912.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8588 a         5522 c         5606 g         8475 t
ORIGIN      
        1 tattatttaa ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa
       61 atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta
      121 ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac
      181 tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa acagtacgac
      241 ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct
      301 tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca
      361 gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg
      421 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
      481 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      541 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      601 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      661 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      721 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      781 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      841 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      901 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
      961 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
     1021 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
     1081 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
     1141 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
     1201 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
     1261 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
     1321 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
     1381 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1441 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1501 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1561 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1621 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1681 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1741 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1801 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1861 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1921 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     1981 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     2041 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     2101 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     2161 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     2221 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     2281 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     2341 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     2401 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2461 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2521 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2581 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2641 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2701 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2761 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2821 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2881 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     2941 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     3001 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     3061 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     3121 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     3181 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     3241 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     3301 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     3361 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3421 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3481 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3541 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3601 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3661 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3721 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3781 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3841 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3901 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     3961 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     4021 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     4081 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     4141 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     4201 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     4261 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     4321 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     4381 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4441 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4501 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4561 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4621 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4681 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4741 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4801 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4861 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4921 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     4981 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     5041 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     5101 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     5161 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     5221 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     5281 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     5341 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     5401 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5461 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5521 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5581 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5641 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5701 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5761 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5821 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5881 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     5941 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     6001 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     6061 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     6121 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     6181 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     6241 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     6301 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     6361 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6421 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6481 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6541 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6601 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6661 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6721 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6781 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6841 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6901 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     6961 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     7021 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     7081 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     7141 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     7201 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     7261 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     7321 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     7381 ttgatttgcc gacatttggc cggccgatga ttgccgtatt taacccttcc cgcagaacct
     7441 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7501 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7561 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7621 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7681 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7741 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7801 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7861 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7921 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     7981 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     8041 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     8101 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     8161 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     8221 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     8281 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     8341 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     8401 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8461 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8521 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8581 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8641 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8701 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8761 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8821 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8881 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     8941 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     9001 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     9061 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     9121 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     9181 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     9241 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     9301 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     9361 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9421 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9481 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9541 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9601 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9661 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9721 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9781 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9841 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9901 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
     9961 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
    10021 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
    10081 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
    10141 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
    10201 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
    10261 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
    10321 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
    10381 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10441 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10501 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10561 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10621 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10681 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10741 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10801 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10861 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10921 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    10981 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    11041 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    11101 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    11161 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    11221 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    11281 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    11341 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    11401 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11461 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13141 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13201 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13261 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13321 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13381 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13441 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13501 agaaaataaa caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga
    13561 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13621 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13681 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac
    13741 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13801 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13861 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13921 ctattcccgt ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt
    13981 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14041 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14101 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14161 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14221 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14281 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    14341 atgattttca atgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    14401 cagaaggccc taaaatctct tttaaggctt tctgtttatt tttgtttaaa tcaccttttt
    14461 tagattaaat cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa
    14521 aggtataata gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt
    14581 cattctaaat acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg
    14641 tagtggcgga caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc
    14701 agaaaatcgt cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg
    14761 aacttttttt atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa
    14821 aaaattatcg aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt
    14881 taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga
    14941 cgcgaagtca tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga
    15001 tgattcaagg agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta
    15061 aaaaaggccg aaaaaccaag atcaaccata tcgaacagaa aaagttaagt acttacgtgg
    15121 gacaaatgaa tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc
    15181 agaccaggcg gaagttttta gatatggaat taggccagat caatccaatc tatctttatg
    15241 atcttgtcca ataccaagca attttgaaac aaagaaacca gtacttgaag caattgaatg
    15301 aaaaaaaaca aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg
    15361 gcagcaaagt attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt
    15421 ctttgcatca gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag
    15481 tcgattcttt agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga
    15541 accaaaataa aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg
    15601 atctttcctt ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa
    15661 gaacaactgc actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacagggg
    15721 aatatccaat ccttttgtta gatgatgtga tgagcgagct ggatgataat cgtcaactcc
    15781 atctgctaga aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc
    15841 atgtaaaaaa taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa
    15901 aaggagaaga aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga
    15961 tgccagtcag attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta
    16021 tatcggttcg actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc
    16081 gatcgatgaa gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa
    16141 cagtatcacc gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg
    16201 acgtcctgca gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg
    16261 tgggtacaaa gtttctggtg gacttcacgg agtaggatct tctgttgtta acgcgctttc
    16321 tacacagctt gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg
    16381 tggagctgta gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt
    16441 tcactttacg ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt
    16501 ggctacccgg atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga
    16561 caaacgagaa gaagaaccag tcatcaatga ataccattat gacggcggga ttaaaagtta
    16621 tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg
    16681 tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa
    16741 tatcatgagt tttgccaaca atatccatac gtatgaaggc ggaacacatg aatcaggatt
    16801 caagactgct ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga
    16861 aaatgacgac aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat
    16921 caagcatcct gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt
    16981 acggacagtc actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc
    17041 aagtgtgggc cgtcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc
    17101 gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg
    17161 aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg
    17221 agattcggcc ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc
    17281 tattcgtggg aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga
    17341 agagatccgg tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc
    17401 gaaagctcgc taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat
    17461 ccgtacattg ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata
    17521 cgtctatatt gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt
    17581 acaaccagga aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc
    17641 accaaaacca agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg
    17701 ggaaacaact atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat
    17761 tgcagcagat cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt
    17821 catcgaggaa aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa
    17881 gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag
    17941 actccttcat cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag
    18001 atggtctgaa acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac
    18061 cggataaagc ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc
    18121 ctcatggaga ctatgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc
    18181 gttatatgct agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg
    18241 ctatgcgtta taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca
    18301 ataagaacac tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac
    18361 tacctgctcg cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg
    18421 ccacgaatat cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg
    18481 ataatcctga tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa
    18541 caggtggttt ggttatggga aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt
    18601 ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca
    18661 tcgtgacaga attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac
    18721 tgcacagaga caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag
    18781 gtatgcggat cgtcattgac atccgtcgtg acgccagcgc atctgttatc ttgaataact
    18841 tatataaatt gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag
    18901 gtgtgccgaa agttttgagc ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg
    18961 aagtcatcac aagacggaca gtctttgaaa aagaaaaagc agaagcaagg gcacacattt
    19021 tagaaggttt gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt
    19081 ctaaatcaga tgacgaagca aaagcgacga tgatcgaacg gttcgattta tccgatcgcc
    19141 agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa
    19201 tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa
    19261 gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg
    19321 gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt
    19381 taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg
    19441 caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc
    19501 acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct
    19561 ttacgaataa cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgca
    19621 cagccaaagg aataccaatt atcaatttac taggtatcga ttcgagtgaa agcatccagg
    19681 caatcattgc ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc
    19741 aaggtacagt aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga
    19801 tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat
    19861 ctaatatcat tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc
    19921 gcgatatggg acgtacagct tccggggtta gaggaatacg cctgcgtgag gaagattatg
    19981 tcgttggggc agccttaatg aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt
    20041 acggaaaacg gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca
    20101 aaacggcgaa catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag
    20161 atgaagacat catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg
    20221 tttctcaaac tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga
    20281 aagttgtcac gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag
    20341 gatcagaaga atagccatca agactaattg tgtaagctag caagaatgac cgattaaaaa
    20401 atctggttat tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact
    20461 gttcattctg tattgctttt gaccaaaata gagtgtataa tcagagcttg tgagtagtct
    20521 aattaattag ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg
    20581 tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat
    20641 gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca
    20701 gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac
    20761 cgtgaaggta tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat
    20821 gaatttgacc gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa
    20881 gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt
    20941 gattaataat gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc
    21001 aagtggtacg gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag
    21061 cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt
    21121 agcaaactat gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc
    21181 ttatgataat cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca
    21241 attgctggaa agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc
    21301 tggtcaatca gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc
    21361 caacggtatg cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat
    21421 cgacatttca gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg
    21481 cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata
    21541 ttgaatatat cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta
    21601 aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg
    21661 caatcaaacg cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc
    21721 taggaattga atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat
    21781 aacctctatt cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata
    21841 tatgggagga aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt
    21901 atcctgttta tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc
    21961 gcttcggtca ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt
    22021 tggcttattt taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat
    22081 ctagcttatg tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa
    22141 aaaatcgaat ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag
    22201 gaagaattgc tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta
    22261 gcagataaaa catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat
    22321 attacccaaa accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt
    22381 ttatctattg ataactatgc agatgccatt gataaattgg atgacaaaga gatttcttat
    22441 ttgaactctt tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc
    22501 aaaagaatca acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg
    22561 atggataaaa aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg
    22621 cctatcaccc ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga
    22681 gaagctcaga ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa
    22741 gaggtaaaag aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa
    22801 cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca
    22861 ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt
    22921 ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt acatcgttat caacgagaac
    22981 gagatcattc cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa
    23041 atgcaactaa tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg
    23101 atggtggatt accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat
    23161 aagattgtga tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg
    23221 acttatatcg aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac
    23281 caaagcagca aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg
    23341 gttgtagata caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc
    23401 tatttaaggt cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta
    23461 gcgacatact tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt
    23521 gttgtggcgg cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca
    23581 gatacattgc tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac
    23641 ggcttgattg ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa
    23701 tccttaggtg gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt
    23761 gaggaaacaa aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat
    23821 aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag
    23881 gggacatcaa agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg
    23941 caaaagaagc aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa
    24001 aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag
    24061 atactgtagt cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc
    24121 cttcaaaaca aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa
    24181 aaatcgaatt agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc
    24241 atcatgaggt gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag
    24301 cgatgttcag caggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga
    24361 aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct
    24421 tttttgggaa cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt
    24481 tattacactt tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt
    24541 gaagacaagg agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata
    24601 ttgaagctga acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg
    24661 ccatggagta catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga
    24721 caatgatcga gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc
    24781 tggaacaagc caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat
    24841 tagcagttcc gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat
    24901 tgctgcgtac attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag
    24961 aagaagttca aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa
    25021 gaaatcgaag cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg
    25081 atcgactagc tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg
    25141 ataaaatgac agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg
    25201 taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt
    25261 ctgttgcgat atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg
    25321 cagaaggttc gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt
    25381 cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac
    25441 cagggatcaa aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca
    25501 atctaggctt gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc
    25561 gtcaacaaga agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag
    25621 tacctgtcat tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac
    25681 cagtgctaag tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct
    25741 tcttgtatcg agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc
    25801 caaaaaatga caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa
    25861 cagttgaact actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag
    25921 aattttaaag aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca
    25981 aggagtaacc gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg
    26041 acaaaagtct tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg
    26101 gtattagctc agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac
    26161 tcaggtcaaa acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc
    26221 ctcggtcaca agtcacaata tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg
    26281 tgactcagag ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc
    26341 atctgcggta atacgcccaa tcaaacgaaa aatatgagaa gctatttttg tttggctgtt
    26401 cttattactt gatgcaggac agctttttca caacctctga ttcacggtgt tccaaaagta
    26461 gcttcttcga aaaataagtc gaaatttcac aaaaatttga gaagcaattt tcgtacattc
    26521 cttcttattt cttgaagctg accacttctg ttacaacctc ttttttaata accattctaa
    26581 aaagataaag ttcgtttttt aagcgttttt ataaaataaa aataaaaatc attcgatttt
    26641 atttgaaata tgtatctatt attgatacaa tggttcagga ttaaataata tagaacgagg
    26701 tgttcacatg tcatctgtag tagtagtagg tacgcaatgg ggcgatgaag ggaaaggaaa
    26761 aatcacagac tttctcagtg aaaatgctga agtgattgca cggtaccaag gtggcgacaa
    26821 tgcagggcac acaattaagt ttaacggtgt gacttataaa ctgcatttga ttccttctgg
    26881 gattttttat aaagacaaga tcagtgtgat cggaaacggc gttgtagtca atcctaaatc
    26941 attggttaaa gaattagctt atttacatga aaaaggtgta gaaacaagta atttgcgtat
    27001 ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa ttggaccaat tacaagaaga
    27061 tgctaagggc gaaaataaga ttggtacaac gattaaagga atcggacctg cctatatgga
    27121 taaagcagct cgtgtaggta tccgtattgc agacttatta gataaagaaa tttttgaaga
    27181 acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt gtaaaaatgt ttgattcaga
    27241 accacttgat tttgacgata tttttgaaga atattatgaa tacggacaac aaatcaaaca
    27301 atacgtcaca gatacttccg ttatcttgaa tgatgctttg gatgcaggca aacgcgtgtt
    27361 gtttgaaggc gcacaaggtg taatgctgga tatcgaccag ggaacttatc cttttgttac
    27421 ttcttctaac ccagtagccg gtggggtaac aatcggaagc ggtgtagggc cttcaaaaat
    27481 caataaagtt gtcggcgtct gcaaagccta tacgtcacgt gtcggtgatg gaccatttcc
    27541 aactgaatta tttgatgaaa caggagaaca aatccgcgaa gtaggacgag aatacggtac
    27601 gacaactggt cgtccgcgtc gtgtaggctg gtttgattct gtagttatgc gccattctaa
    27661 acgagtttca ggtattacca acctatcttt aaactcgatc gatgtattaa gtggattgga
    27721 aacagtaaaa atttgtacgg cttatgaatt agatggcgaa ctaatctacc actatccagc
    27781 aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa gaattgccag gctggtcaga
    27841 agacattaca ggatgcaaaa cacttgcaga actgccagaa aacgcacgta actatgtacg
    27901 tcgtatctca gagcttgttg gtgtacgtat ctcaacattt tctgtcggac ctgatagaac
    27961 acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa ggataaaaac gacgactgaa
    28021 tcaggacgtt cttaccagag cgagtcgaag tgtttatcta tacgataata ataacttgaa
    28081 gaggtagtga aagagacgtt tagccttatt gccgataggc tgtctcttga acactgttta
    28141 ttcagaataa gggactgtga caaaaaccgt gtcacagtcc ctttttccga a
//
LOCUS       DAXWLG010000018        27920 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXWLG010000018 DAXWLG010000000
VERSION     DAXWLG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.018"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(460..2352)
                     /locus_tag="ACGE4T_000605"
     CDS             complement(460..2352)
                     /locus_tag="ACGE4T_000605"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385913.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2349..4076)
                     /locus_tag="ACGE4T_000606"
     CDS             complement(2349..4076)
                     /locus_tag="ACGE4T_000606"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385914.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4092..4742)
                     /locus_tag="ACGE4T_000607"
     CDS             complement(4092..4742)
                     /locus_tag="ACGE4T_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFF8385915.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4946..5242
                     /locus_tag="ACGE4T_000608"
     CDS             4946..5242
                     /locus_tag="ACGE4T_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8385916.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5434..6054
                     /locus_tag="ACGE4T_000609"
     CDS             5434..6054
                     /locus_tag="ACGE4T_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8385917.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6146..7312)
                     /locus_tag="ACGE4T_000610"
     CDS             complement(6146..7312)
                     /locus_tag="ACGE4T_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8385918.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7324..7677)
                     /locus_tag="ACGE4T_000611"
     CDS             complement(7324..7677)
                     /locus_tag="ACGE4T_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8385919.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7805..8827
                     /locus_tag="ACGE4T_000612"
     CDS             7805..8827
                     /locus_tag="ACGE4T_000612"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFF8385920.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8846..9007
                     /locus_tag="ACGE4T_000613"
     CDS             8846..9007
                     /locus_tag="ACGE4T_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385921.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9266..9700)
                     /locus_tag="ACGE4T_000614"
     CDS             complement(9266..9700)
                     /locus_tag="ACGE4T_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385922.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9810..11669)
                     /locus_tag="ACGE4T_000615"
     CDS             complement(9810..11669)
                     /locus_tag="ACGE4T_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8385923.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMVLFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11670..11810
                     /locus_tag="ACGE4T_000616"
     CDS             11670..11810
                     /locus_tag="ACGE4T_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFF8385924.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11787..12946)
                     /locus_tag="ACGE4T_000617"
                     /pseudo
     CDS             complement(11787..12946)
                     /locus_tag="ACGE4T_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13253..14677)
                     /gene="araA"
                     /locus_tag="ACGE4T_000618"
     CDS             complement(13253..14677)
                     /gene="araA"
                     /locus_tag="ACGE4T_000618"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFF8385925.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14693..15391)
                     /locus_tag="ACGE4T_000619"
     CDS             complement(14693..15391)
                     /locus_tag="ACGE4T_000619"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8385926.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15348..17009)
                     /locus_tag="ACGE4T_000620"
     CDS             complement(15348..17009)
                     /locus_tag="ACGE4T_000620"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFF8385927.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17227..18627
                     /locus_tag="ACGE4T_000621"
     CDS             17227..18627
                     /locus_tag="ACGE4T_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFF8385928.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18879..19958)
                     /locus_tag="ACGE4T_000622"
     CDS             complement(18879..19958)
                     /locus_tag="ACGE4T_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8385929.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20044..21003)
                     /locus_tag="ACGE4T_000623"
     CDS             complement(20044..21003)
                     /locus_tag="ACGE4T_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFF8385930.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21015..22526)
                     /locus_tag="ACGE4T_000624"
     CDS             complement(21015..22526)
                     /locus_tag="ACGE4T_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFF8385931.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22548..23111)
                     /locus_tag="ACGE4T_000625"
     CDS             complement(22548..23111)
                     /locus_tag="ACGE4T_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8385932.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23117..23980)
                     /locus_tag="ACGE4T_000626"
     CDS             complement(23117..23980)
                     /locus_tag="ACGE4T_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8385933.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23994..24890)
                     /locus_tag="ACGE4T_000627"
     CDS             complement(23994..24890)
                     /locus_tag="ACGE4T_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8385934.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25065..26354)
                     /locus_tag="ACGE4T_000628"
     CDS             complement(25065..26354)
                     /locus_tag="ACGE4T_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8385935.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26326..27843)
                     /locus_tag="ACGE4T_000629"
     CDS             complement(26326..27843)
                     /locus_tag="ACGE4T_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFF8385936.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8156 a         5875 c         4846 g         9043 t
ORIGIN      
        1 tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa aagtcgctta gtttcgagta
       61 gtaagaaaaa ttttgtgaaa atggctttct acatttttgc ataaatttgg cttaataccg
      121 agaaactggc ttttgaacac cgtttaccaa gaggttgtga gcctgccgtt taactccgag
      181 tcacaaggag caatttcaaa aaacagcttc tcaggtttgt tgactattgt gacttgtgac
      241 cgaggagttg gcaggagaac accgtttaag gaagaggttg taaaatcagc ggtttgacct
      301 gagtattaga gatacaaaac ggaaaaatag cttctcatat tttgccgttt tttgagctaa
      361 taccgaagga cagctgattg aacaccgttt attcggaaaa agggactgtg acacgacttt
      421 tgtcacagcc cctaaaaaaa ataataaatt tgtactattc tatgaagctt cttcttcaga
      481 aaactgactg ttataaagat ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt
      541 tcctgtttct acgatcgaac catgattcat cacaataatg ttgtctgcat cgcgtattgt
      601 agacaagcgg tgagcaacaa cgaaacttgt ccggttttcc aataggcggt tcatcgcttt
      661 ttgaatcagt atttctgttc gtgtatctac actagatgtt gcttcatcaa ggatcaggac
      721 atctggattt gctaagaatg cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat
      781 attgcttgca tcctcgttta atacagtgtt atatccttct ggcaatttac gaacaaaatc
      841 gtctacgtga gcagctttag cagcctcaat gatttgttcg tctgttgcat cgtcgttccc
      901 atatttgata ttgtcataga tacttcctgt aaacagccaa gtatcttgca gtaccattga
      961 aaactgtgcg cgcaattcat cacgtgaaag atcacgggta tctacaccat cgtaacgaat
     1021 actacctcca gaaacatcgt agaaccgttc aagaaggttg atcagtgtcg tcttccctgc
     1081 accagtcgga cctacaatag cgaccatctc accaggtttg acatccaagt taaaatcagt
     1141 cattagcaag ttatctggtg tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg
     1201 tgtatctttt tcttctggaa ggccagattt ttcattggac atctcttctt catccaacac
     1261 ttcaaatacc cgttcggctg aagcaactgt tgattggata gtgttcatca aacttgcaag
     1321 ttgagtgatt ggttgagaga actgtgttgt atattgcaag aacgcttgaa catcccctaa
     1381 tgtcatgttc ccatttgcaa cttttactcc gcctagtaca gcgacgaaca catagcctag
     1441 atttttaacg aaattcatca acggcatgat gattgccgaa ataaattgag ctttccaacc
     1501 agcggcatac agattctgat tttctttttc aaatacttcc tggtcttttt cagtatgatt
     1561 gaatgtttta acaatggtat gaccaccata tgtttcttct acttggttat tcaataaacc
     1621 taaacttttt tgctgatctg cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat
     1681 gatcaagctt aaagggatca tcaataacgc gactaacgtc aattgccagc tgattgaaag
     1741 catcatcacg atgaccccaa tcaatgtgac tgcactagtt acaaattgag tcaaattttg
     1801 ctgcagcgtg cttgcgatgt tatccatatc attgattgct cgagacatga tatccccatt
     1861 tgtatgtgta tcataatagg aaatcggtac acgattcatt ttggcttcca aatcatgacg
     1921 taattcataa actgttcgtt gggaaacacg tgtcataata aattgctgta aaaagttgaa
     1981 tacagcagaa atcaaataca tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa
     2041 gtctatcgga aattttgtta cttgttggcc ttgttgcatc tgttgagcgc cagccattac
     2101 acctttgaat atttctgtgg ttgctttccc caagaccttt ggtgtttgga tttggaagat
     2161 cacggctgct attgctagta caaatacagc gatgatcgca accatacgtt tagacatata
     2221 gccaaacaat cggcgtaccg ttccccagaa atttttaggc ttttcacctt ttacaccgag
     2281 atttctgcca ggtccaccat gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt
     2341 tgttttactc atgctaagtc ctcctctctt agttgtgaat gaacgatttc ttgataagtt
     2401 tcgtttgttg ctaatagttc ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc
     2461 acaaggatca tatctgcatc cattactgta cttactcgtt gagcaactaa aacggtgata
     2521 ctttctttca tattcgtttt caatgcttgg cgaagattgg catccgtttt gaaatccaac
     2581 gctgagaagg aatcatcaaa cacatagata tctgctgttt tgactaatgc ccgtgcaatt
     2641 gccagacgct gtctttgtcc accagagaag tttcctcctc cttgttccac atggctatct
     2701 aagccatctt ccatctctga aacaaaatct ttagcttgtg cgatttctaa tgctttccaa
     2761 atcatttcat ctgttgcatc cggcttacca tactgcatat tgtctcggat cgttccagag
     2821 aacagaactg ctttttgagg aacaaagccc attaattctc gcaaattata ttgagtcatg
     2881 tcactaatgt ccgttccatt tatttggatc gaaccagatt cgatgtcata taatcgaggg
     2941 atcaaattga ctaaggtcgt tttacctgaa ccagtccccc ctatgattgc gacgatctcg
     3001 cccgacttgg cttggaaatc aatatcttcc aatgcaagtt tttctgctcc atgatatcga
     3061 taattgacat gattgaaggc caatgttgcg ttttttccag caaaagaaac tgttttagga
     3121 ttttcagggt catggatacc gattttttca tccaacactt cattaatacg gtcagctgaa
     3181 gcttgtgcac gaggcaccat gataaaaata gcggaaagca tcataaagct gatcaagatc
     3241 tgcatcgcat aagtcataaa tgcaatcaga ttaccgactt ccaaagtcat atctgctatg
     3301 tagtgtccac caaaccaagt gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt
     3361 ggcatcatca atgcgacgat cgtattaact ttgattgccg tatttgtata atctttattc
     3421 gctaaatcaa aacgattttc ttcaaagcgt gtcttatcga atgctcgaat cactctgacc
     3481 cctgtcaatc cttctcgaaa taccaggttt aaacggtcag ttttcttttg catacttttg
     3541 aataatggaa cagcaaagta catgattccg ccgatcaaaa taatcattac tgggatgaca
     3601 actaagaaga tttttgtcaa ctgatgatct ttttgataag caagtatgct cgcgccaatc
     3661 aacgtgattg gtgcattgat catcatacgt aaaaacattt gtgtaaccat ttggatttga
     3721 gtcacatcat tggtcgttct tgtgatcaaa gaggccgttc catatttatc aaacgcatct
     3781 tttgtcagac tctctgattt tttatagaca tccgtacgca attgcttacc cagtttctgt
     3841 gattcacgag tagcaaaaaa tgtatttgcg atagcagcta agatactgat caaagaaaag
     3901 ccgatcatca caaaacctgt atgccaaata taatcgacat ctccttgcgc taccccttta
     3961 tcaataatat tggaggtaag tgtcggcaaa tataaatctg ccagtacttg gatcaccatg
     4021 aaaactgcag ctgcaattgc tgaagtcaag gagatccgtt ttacgatttt tagcatctag
     4081 ttcccttctt tctatttata tactccgttt ttcagccaag aaagtttcaa attgaattca
     4141 ctgattgctt gctctactga gtaatcttca gatgttgaaa ataagcgata cgaatgcgca
     4201 attgttgtaa atacagtatt catcactaat tggatcagta attccaattc atgatcattt
     4261 tttactttca gcttcgtccg atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt
     4321 tctttatgtg gatcgtgagg cgttttttgg cgactcgtat gaggagcgac ttttcttgag
     4381 gcacggtaat ccatgttcat aaacaagtta cgataaaaag aagcattctc gccttccaaa
     4441 atctcataaa tcattttaga aaaataggat tcgaatcctt tgaatagatc accatcggat
     4501 tctttcatta gttgtaacat atctttagag ctgtcacgtc tcattgtctc aaagtaataa
     4561 aaataaagat cctctttatc ttcaaaatac tgatagaaac tgcctctagg gatatccgct
     4621 aatttgacga tattagcaat cgaagcttct tttaagggta ctctagaaaa ctcgattctc
     4681 gcagcctcga ttagtctttt ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc
     4741 atatggattc tcctttcttt caagatgaca cattgtcata tgacgctatg tcattataac
     4801 acctcctacc tgttttgcaa gacatttgca tagttttttt cattatcatc cgatacatta
     4861 aaaaagtgat aagaggaatc cttccatttg aattaatgag agttgcccat taaaatagcc
     4921 acatactaaa caaaggatga gataaatggc ccaatcaatt agtgtccaag acttcaataa
     4981 attgcctctc gatgctgaca gcatcgtttt agatacaaga aataccttag attacacaaa
     5041 aaaccactta agccactcgc tttctatccc cgctgctatc ttgccaagac aattaaaaaa
     5101 tctagatcca aagaaacttt attatattct aagctataca ggcaggcgat cagaagcatt
     5161 agccgatcaa cttactgcta atggttttcg tgctgtttat atcattggtg ggatggaagc
     5221 tcttcagtcg agtgttgctt gaatattgct tgttagattc aaaagaggct gtgacaaaaa
     5281 atcgtgtcac agcctctttt ataccctgtc tttttttagt tatttattct tcaaccttat
     5341 caatatgact tgatagcctt ttctttttcg agtaaactga ttttgttagt catagttata
     5401 acaatcatag caataaaagg agaaggtaag catatggcat ctgttttagt agtcaaaggt
     5461 catcccctaa ctgcagaaga atcgcgcaca gttaaggcat tgacgtcttt tttaacaagt
     5521 tataaagaaa atcatccaga tgatgaagtg actgttttgg aactttatcg cgatgatatt
     5581 ccggaaatcg atgaagaatt attatcaggt tgggaagcat tacgtgctgg tgcagaattc
     5641 acttccttat cagaaagtca acaacaaaaa attgctcgtt tcaatgaatt gactgatcag
     5701 ttccttgctg ctgataaaat cgttatcgca aacgccttat ggaatctaaa tatcccaaca
     5761 aaattaaaag cttggttcga taccgtcaat gtagctggaa aaacgttccg ctatacagaa
     5821 aacgggccag aaggattagt gacaggtaaa aaagcactgc acatccaatc taatggcggt
     5881 gtatacaacg gacaagactt tgcctctcaa tatgtaaaag gtattttaaa ctttgtaggc
     5941 atcgatcaag tagatcagct attcatcgaa ggaatcgatt atgaccctga ccgtgcagat
     6001 gagttgatgc aaaatgcatt agataaagct gctgctttag gtaaatcatt ttaaaaattg
     6061 agataaatgc atacaaaaga gacagaggaa aaaatcttca aagcagcatt tgtgccttgg
     6121 atattttccc atctgtctct ttttgttatt ctttctgctt tttcaacgga ttttcttcat
     6181 aaagagaaga gattgttgtg taccattcaa aaacatgagt aatcactact ttagctgctg
     6241 cataaacagg aatccccaag atcacaccga tgattccgaa caatttccca gaagtcagca
     6301 gaactaataa aatcgttact ggatggattg ctaactgatt ccctagtact aatggagaga
     6361 tcaagcgtcc ttctattgtc tgctctactg caaaaacgat cagtactttg atgatcatga
     6421 atggacctgc tacaatggct aaaaagatag ccggaatcat cgctaaaaat gatcctaagt
     6481 aagggatcag gtttaaaaaa cccgcaataa tccccaacgt gacagcatac tctaaaccaa
     6541 tgaccgcaaa accaatcata aacatgattg ccacagcaaa agccacagtt agttgtcctc
     6601 ttatataaga agaaacttga ctattcattt cactcagcac attcaacgtc ggtttttgca
     6661 tgcgtgtcgg taaaaatttg acaaaataag gtgctaattg tttaccgtct ttcaatagat
     6721 aaaataagat aaacggcatc gtcagcagag ccactaaaat agtcgctacc gcgccgacaa
     6781 aacttccaag actctgaact gtactcttag atatatcttg gatcatatcg cctgccgaat
     6841 tcatgaactt atcacctgct gtttccaact gttcgcgaaa ttggttgaac agcgggtccc
     6901 gcaaaatctc ggttaattta ttatcaatcg tatctacata ttgagggaag ttactgatga
     6961 agctcactgt ttgttcctga atcttaggga tgatcactac gctcccccat gcgattagtg
     7021 caacaaccaa aacaaacagc ccaatgatac tgtaaagcct tggtactttt cgcttttcaa
     7081 agtaatcaac taccggattc atcaagtaat acaagattcc tgctaaaatg ataggcagac
     7141 ctacgattgc taaaaattgc cagattggtg caaacaaata tgaaactttt gtaaataaaa
     7201 agacgatcag caatattaac aatacaacaa gtaaggctgt gactacttga ttatttaaaa
     7261 accagcgcca aaaccacgat accggtctgt tcttcttatt ttttccttcc ataattcctc
     7321 ctgttagtta taaaaaattt ggcttcccaa accaatagca ggcagtctat gcggatgaag
     7381 atagattcca tttgcaattt gtccttcttc tggacaagca tcaaaaatca atgatacgtg
     7441 agctactgtc tttaaattcc cattggccat cggaccgaca taatcgatcg tatatacctg
     7501 atcatcaaaa gcaattgttc cgcctgcttt caaagaaaaa ggttcgtctt ttgatacttc
     7561 ttgaatcacc gaatattttc gtaaggcagc tgttgctgtt ttatcgaaca aaatcagcat
     7621 gggttcttcc ttgtttattg cttctgtccc aatttctgta atctttgcct gaatcattgc
     7681 actctctcct ttaattgctt tcaaaaacag tataacacat tctcattctt tagccgtgtg
     7741 gaataattat gtgatagaat aaaaacgaaa actatttcat tacaattagg aggaatcagc
     7801 tcatatgatc gaaaagataa ttttaggaac ctatacgcgt cgcgaaagta aaggaatcta
     7861 tacgatcgac cttgatacag aaaaagaaga attagccaac ctttcattag ctgttgaaga
     7921 aaatagccct acctatgtag caaaaagtaa agctggcaat ctttacacag ttactagtgt
     7981 ggatggatta ggtggtgccg gtgcgtacga taaggatttt cattttttga atgcggtaac
     8041 agagtctggc gctcctttgt gttatgtagc ggttgatgaa gcacgtcaat tactatacgg
     8101 ggcgaactat cataaaggtg aagtaaatgt ctaccatata ttaggagatg gcgaactgaa
     8161 agcagcagat tcaatattcc atcaagaagc gacaggtcct cataaaaacc aagatcatgc
     8221 gcatgtccac tatacggatc tgactcctga ccaacgatta gtcgtatgtg atcttggtac
     8281 agatcgtgtg tatacttatg atgtgtccga aaacggcaaa ttgaatcttg taactacctt
     8341 cactgcagag ccaggaacag gccctcgcca tcttgtcttt catccaaatg gacaattcgc
     8401 ttatttattt ggtgaattag acagcacagt ccgcgtgctt tcttataata aagaaacagg
     8461 tgcttttgaa gaattacaga aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg
     8521 aggggcagct atccgcattt caaatgatgg caagttcctt tatgcttcta accgaggaca
     8581 taactcgatt gctgtattcg cagtttttga agatggttcc cacattcaaa acaagcaaat
     8641 catctcaaca gaaggggatt ttccccgcga tttcgcacta gatccaagca ataattttgt
     8701 cgtgtgtgcc aatcagaaca ccgataatct gacattatat cgccgtcatc ctgaaacagg
     8761 attactggaa ttactgaaaa aagatgtcta tgtaccagaa tgtgtctgtg tttattttga
     8821 tcgttagtaa gaaaaggaga atgaaatgaa tcagcaagac gtattagaca gactacggga
     8881 agaactaaat attcctttct ttgatgggaa aatcgaagat aaagagtata gcgaagagga
     8941 atatcagaaa ctcaaagccg atttacagaa ctactttgaa caatatgtac agaatgtaga
     9001 aaactaatca gctttccata ctatctgaaa agaacatcag gacaagactt ttccgtctat
     9061 cctgatgttc ttttctacaa ataaaaaacc gccgagatgg ttagactcga cggaaaagga
     9121 gtatttactc gattttgagt aagtacaagg ttgttgttgg ttataggtat gtcattatca
     9181 taaaggattt tgatcactct atcataaagg taatattaca tttatattag ataactctag
     9241 tttttattgt tatcaaagat ttcttttatt ttttcaacaa caaattagtc attttttgga
     9301 tggcagctgt caattggtct gtcgcttggt cttgttttgc ttcagatgta ttgaatagat
     9361 cttcatctac ccctgattca agatcgatac tttggatcat ttcttcacag cctgctacat
     9421 atgaaagata agctttttca aaatttttat gaatgcccat tgcttttgct ggtggacgca
     9481 aagtactgac tttcataagc attgcccgat atttttttgt tccttcttca aataacagga
     9541 tgatctcttc aagagtcgat ttgtccagtt cacttgtttt tccttcatca attgcgtttc
     9601 tgatcacttc atatttcgga tgcattcctt ctccgattgc ttctgtttcc ttgacgattt
     9661 gatcgatgga ctgcacataa tagccaatat tcggtaacat gttcgttttc ctactttctc
     9721 cattgttgtt attatttatt ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa
     9781 tttaatggtt tctatacagc tcgccattct tattcgtgct caaagaaacc atctgctaaa
     9841 ctatattgtt tccctgcgac aatgtcatat tggatcaatt gatattcgtc gtaaatctct
     9901 tgttgcgagc gattgaattg cgcttcttta taccactgtc cgttttgata gtacagttct
     9961 cgttcaaacg ccggaagttc ctgttctaat gataaaagca gctgataaaa gccatttgtt
    10021 gtctgattcg tctgttccat tagattcgct gcaaaataaa acggactggt gattgcatcg
    10081 tgagtcgttt gcttttctaa ttctccttta ctgtcgaaca taagaaattg tgtttcatgc
    10141 aatgtttgtt tgtcattttt tgcttggatc gtatctgaat agatccctgg aagatgatcc
    10201 ccccaaaaaa caactaaagt ccttctagaa agattcttca attcttccgt gaactgcttc
    10261 agtgccgtgc tgctatagga aatatcttgc agataattct ctaagtcaag tgtcccactt
    10321 ccatctgaag tttttgcagg atagtctaga ttatcatact ttccatcgta gggcatatgt
    10381 gtctgcatcg tgactaaatg aatgaactga ggtgtttttt cttccttgag aagtgtcatt
    10441 acttctttgt aagctgactc atcagaaata tatggattat tctctatcgt atccgtatag
    10501 gtcatcgtac gctcgctgat gaactggtca aatcctaatg tctgataaac atcctctctt
    10561 ttatacatcg aagtattgta tggatggatc gctgtcgtct ggtatgattg tgcgtttaac
    10621 gctgaaacga tagaaggaag ctgatccatt tttggtacaa gcatcgtgta aggggtggtg
    10681 agttgcgcat taaataacac catagaaaaa ccagtcaatg cttcaaattc gatatttgct
    10741 gttccaccgc catagttttg agaaagcata ttgccactat atgtttgatc agctacttca
    10801 taataatctg ccaatggctc accagatact tctacgccgt ttaatcgact aggatctgag
    10861 aaactttcac tcatcacgaa cacgatgttc ggcgactctt cctctacagt tttatttttt
    10921 tcatcggcta gcttctggta tttttcagtg atttctttga tcttttcttt cgaatatcct
    10981 tcaggttcat ccattggttc taccttcaga ttgtacaaaa acccaccgat aaaaccagtg
    11041 ttatagtagt tcattttctg gctgtaaggt atccatagtg ccgttttatt atatgctttt
    11101 cgcaacaaat tatcgggatt gttgaaatta ctgatatacc ctagcagtcc aattgccaca
    11161 agcagtgtcg ttaatcggat caattggaat cgccttcctt ttttcaggct tcgaaacatg
    11221 taccagcaaa acaatcctat cacggttcct gcggcagcta agatcactgt aaacagcata
    11281 gtgccggtca tatctttcag caatccgatt tcagtgatca tcttcaaatc gtctggataa
    11341 atcggttctt gccgataact cattttcata taatttgcaa aacctaatac accgatggct
    11401 acgacatacg taagggaacc taaagggaca gatcctgcta gtgcaatcag aaaaataaga
    11461 ataatcagca agacgagaca ggctaagaag aatttttccg tatgccaaga aaaagcaaat
    11521 ttcaacgcaa ggtctacgga tagatcattt tgacaccact gcaaaaataa attactaaaa
    11581 acaacaagaa aaaagatact gatgattgct gctgttcctt ttaaaatctt tttctgtttg
    11641 gtcgaaagtc tgcttatata tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag
    11701 aatgttgctt ctcttatttt agttgtacga aattcgaaag taaagaacgg aaagaagaat
    11761 taatgaaatc caaatattct ctttctttat ggcctttctg gtttctttaa taagcggacc
    11821 tttcgtactc cagaaatttt ttctagctgt tgtttcactt gattgagctg ttcaaaatca
    11881 gtactgtcta tgtcaatcaa tatgtagaca tgctcattct tatggttacg cgatatatct
    11941 gcgatcgcta gctcgttctc attcagcaga gaaaatatct ctgctaatat attggttttc
    12001 ttttgataaa atatagtgaa tcgaaacggc gcttggaaaa acattcgtgc ggatggataa
    12061 ttgaccgctt ctctaaccgt accgaacaaa agataatctt ttaaggcttc aactgccaaa
    12121 cgtccgctgt ctgccaaagc ttcctgagtc gatcccctag atgaggcaac ataagtatac
    12181 ggtcattttc taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt
    12241 tttccaatgc ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa
    12301 gtacagcatc ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt
    12361 ctgtcaacgg cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg
    12421 cttgccgaac ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta
    12481 agctatagca acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg
    12541 ttttgccttg caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa
    12601 tattaggacc tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata
    12661 agagcaaaca gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca
    12721 tgacgatcgt cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag
    12781 ctcttgaaat tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa
    12841 taactattcc ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt
    12901 ctatctcttg ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt
    12961 actaacaagt atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga
    13021 agcaaaaaga gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt
    13081 actgccataa gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg
    13141 tgttccgtta gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt
    13201 cgtcaatctc ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa
    13261 taaccactgt ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat
    13321 ttagcgagac aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat
    13381 ggaaattcgg tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg
    13441 ttggttcaaa ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca
    13501 tcgatacgac taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc
    13561 tatctcccat tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat
    13621 ctacttccat catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat
    13681 aatcttccat gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg
    13741 ctgcagtttt ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta
    13801 cagctagtcc aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt
    13861 tatagcctcc ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta
    13921 catgttcctc ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt
    13981 actcttcaaa taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat
    14041 ctccgatacc aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc
    14101 cttctgtgac acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac
    14161 tttcattata ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc
    14221 gtttccagta gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat
    14281 attcgcggtc accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc
    14341 aaggaatgct ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg
    14401 tcccgcggat ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta
    14461 cttcttctcg ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta
    14521 attcttgaag gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt
    14581 cttgtgcatg tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta
    14641 caacaaacca aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt
    14701 tttgtccgta atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt
    14761 gaggcagctc ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt
    14821 catctagtac gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac
    14881 cgtgtccgtg taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg
    14941 tttctatgat aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca
    15001 aaaatcgagc acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag
    15061 gaacatccaa tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc
    15121 ctccgatttg aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat
    15181 tgagtttacc ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca
    15241 tctcttcata actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag
    15301 cgctcacatt tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt
    15361 ggtatacttc ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga
    15421 tgaatcgctg ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag
    15481 cctgctattt cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct
    15541 tctgccaaga agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac
    15601 gcgattcccc acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata
    15661 gcagatgcta gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact
    15721 aaacgatcaa tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca
    15781 aaagcactgg ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta
    15841 cgtacaaata atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca
    15901 taagaaagta atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg
    15961 aataatttat ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg
    16021 acccaagcat tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct
    16081 gaaggtgtag tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg
    16141 atcatagcga aggcggaagt accagccgaa atgttccctg tccctttggc tacactgttc
    16201 gtagcgacca taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc
    16261 aaatttcccg tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct
    16321 cctgctacta gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg
    16381 attaactgac caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata
    16441 ggaaacatgc ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg
    16501 acataacctg caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa
    16561 atagattgat acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc
    16621 gttaatattc gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt
    16681 aactgttcct tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac
    16741 ccgattgtcg taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa
    16801 ctgacgcgca gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt
    16861 ttgttttccc actcataact accagcggca ataggagtaa aagatgaatc aatcaataca
    16921 gctttgatcc tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct
    16981 aacatacttt gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc
    17041 tgatcaaacc gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat
    17101 gtcaagcagt tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta
    17161 taaaatgaaa ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga
    17221 atacttatga aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt
    17281 ggcggcattc tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag
    17341 catgattggg gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg
    17401 ttaggtgcga tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga
    17461 aaaatgattc ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc
    17521 cctcacaatg gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc
    17581 gctgcatctg ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga
    17641 agattgtcag ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca
    17701 gattatttgc taaaagggtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct
    17761 gctgtccccg cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt
    17821 cttatgcaat ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca
    17881 aaagaagctg aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc
    17941 ggaacttctt ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt
    18001 ggtgtagcag tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg
    18061 atcgtagaaa aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa
    18121 ggaatcattt tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg
    18181 cgaactttat tgaaaatagg cggtagcgtg atgggacttt ctttcatcct accagctgtt
    18241 ttaggaactg tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt
    18301 tatgtcgcat tttattcttg cacatgggct ccactgacat gggtaatcgt aggcgaaata
    18361 ttcccactag ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt
    18421 tctttcctag ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt
    18481 ttcggcattt ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca
    18541 gaaactcgtg ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct
    18601 aaacgtattt ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa
    18661 ataagaagga accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat
    18721 gtttgatgaa tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt
    18781 ggtgaaagaa acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt
    18841 aaccgtagga gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact
    18901 gtttcccgtt caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca
    18961 atcgttgtga cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat
    19021 gttgtcaatt ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat
    19081 aggtcatctg gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt
    19141 tgatcgttat agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt
    19201 ttttcagcga ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa
    19261 cgcacattat actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga
    19321 tcgtcgattt ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct
    19381 gccataaaac cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa
    19441 gcagcattga tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag
    19501 tttgggttat actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca
    19561 atcattttct tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc
    19621 aatgaatacc cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata
    19681 taatcagaga gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt
    19741 tctttcttat agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc
    19801 tcatttacca acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct
    19861 tgcagcttga attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt
    19921 tgttttatct cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg
    19981 tacgtacata tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg
    20041 tgctcataag gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt
    20101 aaagcattgc gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt
    20161 ctctggtcga gcatgataga tcaaagtatc aaagcacccg tcttctgaaa ccgtaaacga
    20221 gttgtgacca ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa
    20281 tttatgccat gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat
    20341 ggcatagttt tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt
    20401 aaggactgct ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat
    20461 agaaaggagg atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag
    20521 attagaattc ccagtaattt caggatcttg ttgggcccac acataatata gctttccttg
    20581 agaagagaaa gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt
    20641 ttctatccaa tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg
    20701 atgatgagat tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt
    20761 tccattgata taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg
    20821 agcctgccat acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct
    20881 taattcaatc acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg
    20941 tttatatacc caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat
    21001 cataacaaat ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc
    21061 cctacattta attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt
    21121 tctggttctt cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct
    21181 ccttgatcta gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga
    21241 ttgatcaaga aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga
    21301 acatttttta tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga
    21361 cctttagcat atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt
    21421 cctttagcat ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc
    21481 tttacgcgat ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct
    21541 tccatagtaa agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt
    21601 tccggttggg catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc
    21661 aaatatcctt ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga
    21721 taagcttttg caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag
    21781 tcttcttcat catacttatc aatatagtta tgcaaagcta aataatctac attctcataa
    21841 gcttcttcta atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt
    21901 ccaactaaga ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt
    21961 gctaacctac catattcata agcactctta gcccccactt gccatggacc gtctagctca
    22021 ttccctaaac accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa
    22081 tcactccaga aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca
    22141 attccttttg ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat
    22201 ttaaaaaatt cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt
    22261 ttttttggtc gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag
    22321 tttcctccag gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct
    22381 tgtctaaacc cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg
    22441 acacttccca tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta
    22501 aaatcctctc tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt
    22561 cctatagtca agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa
    22621 ggagggaaaa gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt
    22681 ctcaaaaaat caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata
    22741 tctttgtttt cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa
    22801 actacatatc cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga
    22861 aaataaataa tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt
    22921 ccttgaaaga aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca
    22981 taaaaaacag cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag
    23041 agaataccta ataagatgta atcagaaacc acaatcaact gtccataaag tttagtctca
    23101 actattttca tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt
    23161 tctgtgcaaa aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta
    23221 caggtccata aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc
    23281 tcatttttga actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca
    23341 gcgtgaaaat ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa
    23401 aaatattaaa ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag
    23461 taataaaaaa ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca
    23521 aacctgcata agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga
    23581 agacttggaa tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt
    23641 tgaaacgcat acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca
    23701 ctgtccctcc agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc
    23761 aaaccttaat atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga
    23821 taatgtcttt ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa
    23881 ccaacaatat aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc
    23941 gactattcac agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc
    24001 atttgttttt ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg
    24061 ataaacaaaa ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct
    24121 cgactgtaaa tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca
    24181 acatatactt ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct
    24241 aaggagggaa cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca
    24301 tccatttgag cagattcata caaagaggga gagattccct gaatagcagt caagagaatc
    24361 gtcatagtaa caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg
    24421 gttggatcgc gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc
    24481 gcccctacat tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa
    24541 ataactggaa tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat
    24601 gctactgctg ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat
    24661 aaagaatgaa tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca
    24721 aaacttacaa aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat
    24781 gttcctaaaa gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac
    24841 aaatacgcag cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc
    24901 tcatctttca ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc
    24961 gaacaatcgc tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag
    25021 atatctgtat cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat
    25081 tgattgatgg catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat
    25141 ttttcaatca gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt
    25201 aaatatggct ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt
    25261 acttcttcat cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat
    25321 tttttaccaa tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta
    25381 ttattatctg tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg
    25441 tctgaatcac tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat
    25501 ccgttattta accatggccc attgatttca atggctagtt gaccagcatt cattaaattg
    25561 tccatttcgg cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg
    25621 atcgctttta acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg
    25681 ttcactaatt taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta
    25741 ttgaagacaa aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc
    25801 aattcttccc aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca
    25861 ttattccaat acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct
    25921 acttcaccta attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc
    25981 cagaaatcgt ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa
    26041 tttaatgcaa taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc
    26101 aaattggccc aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta
    26161 aattcattaa cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa
    26221 gtcaaagtca cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct
    26281 aaaatacctg ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt
    26341 tgttcctcct tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa
    26401 acaattgatt ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc
    26461 acttttagga agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac
    26521 tccctctaat gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt
    26581 aactgcaaat atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac
    26641 ttcttcaaaa tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc
    26701 ttgtccatac acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc
    26761 gtttgtttct gtcctgattg gtgcaataac atttaccaat tgagcaagac aagcaatctt
    26821 tactctatct gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc
    26881 aaaattatag ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc
    26941 ttgttccgct gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt
    27001 actatgtttc ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc
    27061 catgtctaag gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg
    27121 atgcagagac aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc
    27181 accaaatgta ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc
    27241 tacgagtttc atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt
    27301 atgtccaatc tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa
    27361 agggctctct tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt
    27421 acaatattcg actaaattac gagctgcatc tactccacga gtacccagat tgacagccat
    27481 attgatttcg gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt
    27541 cgtttcaatt gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat
    27601 actgtcttcc caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat
    27661 gttcaattct tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg
    27721 atgatctggc tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact
    27781 gtacaaccgc ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc
    27841 cataactgac ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat
    27901 aatataaatg ttaaacttac
//
LOCUS       DAXWLG010000019        27877 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXWLG010000019 DAXWLG010000000
VERSION     DAXWLG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.019"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(424..1368)
                     /locus_tag="ACGE4T_000630"
     CDS             complement(424..1368)
                     /locus_tag="ACGE4T_000630"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8385937.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2608)
                     /locus_tag="ACGE4T_000631"
     CDS             complement(1664..2608)
                     /locus_tag="ACGE4T_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8385938.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2829..4043
                     /locus_tag="ACGE4T_000632"
     CDS             2829..4043
                     /locus_tag="ACGE4T_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8385939.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4065..6080
                     /locus_tag="ACGE4T_000633"
     CDS             4065..6080
                     /locus_tag="ACGE4T_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HFF8385940.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6163..7536)
                     /locus_tag="ACGE4T_000634"
     CDS             complement(6163..7536)
                     /locus_tag="ACGE4T_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8385941.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7562..7846)
                     /locus_tag="ACGE4T_000635"
     CDS             complement(7562..7846)
                     /locus_tag="ACGE4T_000635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8385942.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7848..9863)
                     /locus_tag="ACGE4T_000636"
     CDS             complement(7848..9863)
                     /locus_tag="ACGE4T_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8385943.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELWSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10042..10347)
                     /locus_tag="ACGE4T_000637"
     CDS             complement(10042..10347)
                     /locus_tag="ACGE4T_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFF8385944.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10436..11467)
                     /locus_tag="ACGE4T_000638"
     CDS             complement(10436..11467)
                     /locus_tag="ACGE4T_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8385945.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11674..12549
                     /locus_tag="ACGE4T_000639"
     CDS             11674..12549
                     /locus_tag="ACGE4T_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8385946.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12701..13543)
                     /locus_tag="ACGE4T_000640"
     CDS             complement(12701..13543)
                     /locus_tag="ACGE4T_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8385947.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13635..15512)
                     /locus_tag="ACGE4T_000641"
     CDS             complement(13635..15512)
                     /locus_tag="ACGE4T_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8385948.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15509..16930)
                     /locus_tag="ACGE4T_000642"
     CDS             complement(15509..16930)
                     /locus_tag="ACGE4T_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8385949.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16933..20322)
                     /locus_tag="ACGE4T_000643"
     CDS             complement(16933..20322)
                     /locus_tag="ACGE4T_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8385950.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20579..21970
                     /locus_tag="ACGE4T_000644"
     CDS             20579..21970
                     /locus_tag="ACGE4T_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385951.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22043..22690)
                     /locus_tag="ACGE4T_000645"
     CDS             complement(22043..22690)
                     /locus_tag="ACGE4T_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8385952.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22698..23384)
                     /locus_tag="ACGE4T_000646"
     CDS             complement(22698..23384)
                     /locus_tag="ACGE4T_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385953.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23390..24463)
                     /locus_tag="ACGE4T_000647"
     CDS             complement(23390..24463)
                     /locus_tag="ACGE4T_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8385954.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24685..25359
                     /locus_tag="ACGE4T_000648"
     CDS             24685..25359
                     /locus_tag="ACGE4T_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8385955.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25364..26704
                     /locus_tag="ACGE4T_000649"
     CDS             25364..26704
                     /locus_tag="ACGE4T_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8385956.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26853..27839)
                     /locus_tag="ACGE4T_000650"
     CDS             complement(26853..27839)
                     /locus_tag="ACGE4T_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFF8385957.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8135 a         5268 c         5046 g         9428 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2341 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2401 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2461 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2521 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2581 gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga
     2641 ctccctcctt tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata
     2701 tgcaaagcta tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga
     2761 ttccgctatt gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg
     2821 aaaggaacat gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta
     2881 ttttgacttc tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca
     2941 cgcgttcttt atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt
     3001 ttgttttgat tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg
     3061 gattagtgat tgcatcgatt tatggtatcc tccccctctt ctcacaaagt tacagtattg
     3121 tcttagcttc aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca
     3181 gcatgattgc tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg
     3241 catttcaagg atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg
     3301 gttggcaata taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt
     3361 tgtttgttcc tacacctgaa acaactgaac ttcaatcaca tggcacagcc ccaacaagac
     3421 aagtaatcaa aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg
     3481 tggtatataa tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa
     3541 acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag
     3601 tttgttttgg ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac
     3661 tgatgggaat cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg
     3721 gtgcgatcct aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa
     3781 gtatccaagc gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta
     3841 atttaggtac tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga
     3901 gtaaaacact tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta
     3961 gccttctagt agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa
     4021 aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac
     4081 ataaagatag atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa
     4141 atttccaacg ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc
     4201 atcggaacgt caagtttaag agatattact ttcttgatca accttccatg tttaaagtga
     4261 cgatccaagg agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc
     4321 tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac
     4381 cagcgatgtt tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt
     4441 ttattgaaga atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa
     4501 atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag
     4561 gaatcttatt cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg
     4621 accatatcga atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg
     4681 attattgcta ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt
     4741 ttcgctattt gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg
     4801 aatttttatc tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga
     4861 tctggtcagt atcagaagaa accttcaagt tatgtagtga tttgtttttt atcgacggta
     4921 tcaaacgaga tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat
     4981 atcttttttt tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg
     5041 aaatcaagca gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg
     5101 aaaaccacta tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc
     5161 aatcattaat gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag
     5221 caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag
     5281 aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg
     5341 aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat
     5401 tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct
     5461 atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt
     5521 ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca
     5581 cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac
     5641 agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg
     5701 cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt
     5761 atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt
     5821 taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac
     5881 aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga
     5941 tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag
     6001 tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct
     6061 attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc
     6121 ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga
     6181 aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa
     6241 agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag
     6301 tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa
     6361 tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa
     6421 taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa
     6481 gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat
     6541 ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata
     6601 tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat
     6661 cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac
     6721 aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt
     6781 gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat
     6841 cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat
     6901 ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga
     6961 agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt
     7021 cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc
     7081 taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag
     7141 acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct
     7201 agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta
     7261 ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag
     7321 agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc
     7381 gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt
     7441 cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact
     7501 gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt
     7561 tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg
     7621 attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct
     7681 caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt
     7741 caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca
     7801 taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct
     7861 tttcttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt
     7921 tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg
     7981 atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca
     8041 ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca
     8101 tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg
     8161 atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca
     8221 aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt
     8281 aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta
     8341 cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc
     8401 agtagcgccg accacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct
     8461 ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca
     8521 tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa
     8581 gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc
     8641 gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag
     8701 acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg
     8761 ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt
     8821 tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt
     8881 tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc
     8941 aagtgataaa agacattcat caataattca cgaaacgaag caaattctac ggccatcagg
     9001 aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc
     9061 tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt
     9121 tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga
     9181 gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt
     9241 gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat
     9301 agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt
     9361 gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga
     9421 ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta
     9481 gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac
     9541 aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt
     9601 gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct
     9661 tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata
     9721 cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt
     9781 gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg
     9841 agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta
     9901 gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta
     9961 ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt
    10021 ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc
    10081 aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc
    10141 attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc
    10201 tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt
    10261 agttcctgct aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt
    10321 gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca
    10381 tcgtacaata agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata
    10441 tttttccttc tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga
    10501 ttgtttcttc atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt
    10561 cgtagacacg gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt
    10621 gttcatctat ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat
    10681 gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga
    10741 acatcccttt ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg
    10801 attttatcga aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc
    10861 gtcctttccc taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag
    10921 aaagaacttg atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa
    10981 attcatgcgt attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta
    11041 aaatatcccc taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga
    11101 accgtctgtt ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact
    11161 cttctgcttc ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt
    11221 cgagtgccat ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt
    11281 ggatctcagg atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg
    11341 ttttccacac tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt
    11401 ttcttgaaaa aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg
    11461 taagcatgag tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa
    11521 agtatttata atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt
    11581 tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg
    11641 tttatcagta agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa
    11701 caagagttgt ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa
    11761 gctgcagtct acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct
    11821 atcattcgtt tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg
    11881 gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct
    11941 ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag
    12001 tcaataaaaa aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta
    12061 ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg
    12121 aaattccaga ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt
    12181 gattcttctt ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac
    12241 agcggtcgca tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct
    12301 accttgctga ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt
    12361 gttatatcgc aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc
    12421 ttcgattacg tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat
    12481 atccgcaact tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg
    12541 aaaacttaag aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt
    12601 tgatatcatc ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac
    12661 ggaacgattc gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt
    12721 tgaacgattg acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca
    12781 atccatacag gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt
    12841 ttgcatgata aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag
    12901 gtgtacaagt taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat
    12961 cagtcggttc taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt
    13021 cgatatagaa agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag
    13081 gcagtcctcg atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg
    13141 ttccttccaa taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg
    13201 gcaaatggac attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg
    13261 ttctatcctt tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac
    13321 cttcttttgc taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt
    13381 cttcattttc tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat
    13441 tattcagcgc gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga
    13501 aaagcatcag cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga
    13561 tgactgtttg ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa
    13621 ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc
    13681 agtagaagga ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga
    13741 agtgtacctt tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca
    13801 taagattgtt catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc
    13861 gctttcgttt cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg
    13921 ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt
    13981 gtggatgggt cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat
    14041 tctgctccag caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg
    14101 cccccagtaa cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca
    14161 tttgcttggt tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa
    14221 acgaatttca atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc
    14281 gtgaatccgt ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca
    14341 tcatataagg cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca
    14401 tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata
    14461 cctaatggga tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt
    14521 ccattcaatt gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct
    14581 actggtgttt ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt
    14641 tccgcattat caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta
    14701 acgatcaact ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa
    14761 gaatgaccag taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca
    14821 tctgtgacac tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata
    14881 aattctgctc catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt
    14941 ccatcattac cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg
    15001 tatttataag agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg
    15061 tttgcggcag ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc
    15121 ccattttgtt ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa
    15181 gcaactggtg taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct
    15241 cctttatctc gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg
    15301 gctaatcctt ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga
    15361 tcgggtaaat cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca
    15421 aacacttttt ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct
    15481 aacataacgt ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa
    15541 acaaccagat tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg
    15601 tttcgtttgt ttttggaaga aatggacggc tattttcttt tttattgact actttcaata
    15661 tactgtccgc ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt
    15721 cacttaaaat ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca
    15781 acccggaaat tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt
    15841 tctttgtcca cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa
    15901 taaggaatac tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt
    15961 gcttactccc tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg
    16021 ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag
    16081 aaatggtcag tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa
    16141 atccgtgagg tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa
    16201 tcgttaaact attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact
    16261 tttgatagtc aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac
    16321 caatcgtaaa atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt
    16381 cgttttcttc atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat
    16441 gtacaggaag aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg
    16501 attcaatgaa aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag
    16561 aagcgatacc atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg
    16621 aagacgcacc gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc
    16681 gtagatggta aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc
    16741 gatacgtttg taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc
    16801 cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg
    16861 ctacctgagt aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa
    16921 gttttttcat atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa
    16981 cgcacaacca atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg
    17041 taatggtact tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg
    17101 tgacagaaca gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac
    17161 tatagtaact ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt
    17221 ataagttcca ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg
    17281 cgattctaag tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc
    17341 gttgtcttct tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag
    17401 cgtccataca ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt
    17461 atcgatttgg aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc
    17521 aacgactcga tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt
    17581 tgtttggctg tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc
    17641 tgctccttta agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac
    17701 aggaacttca acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac
    17761 tgtttttcca tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt
    17821 tttgcttaat ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt
    17881 ttgcaatttg acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt
    17941 atcaatagaa tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga
    18001 ggaatttttc gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat
    18061 tgcatctatt tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact
    18121 gaatgcttga ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc
    18181 gctatttgca gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc
    18241 tggtttaatg attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg
    18301 ttgaatctca aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca
    18361 taactttttg atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc
    18421 taattcattg gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa
    18481 atttggatgg aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc
    18541 ttggtctttt cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga
    18601 tggagtgacc gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata
    18661 aatgaatggt tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg
    18721 tacagctttt ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta
    18781 aagatcgcct ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt
    18841 tgagagacca gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc
    18901 tcgttctttg agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct
    18961 ggatagattg ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg
    19021 ccgatcttgc gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac
    19081 ccgctgtact ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt
    19141 atgtccattt ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt
    19201 accaccattt gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga
    19261 acccatctgt actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc
    19321 catattgatt ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc
    19381 aatctttact tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa
    19441 tacgagatca agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc
    19501 aaacaatccg ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt
    19561 accggttcct ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga
    19621 atttatacca tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt
    19681 atttcctgta ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc
    19741 tgtaggaatc gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt
    19801 aaatgggctg acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt
    19861 tggtactgac acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt
    19921 attatcgacg atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact
    19981 cagatcgata gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac
    20041 ctgataagca tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag
    20101 accaatttct ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag
    20161 tgcttttttt ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg
    20221 atcatctgta gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat
    20281 cagcatgaaa ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct
    20341 ttttttctgt tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt
    20401 tatttatttt aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt
    20461 cataccaatt ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa
    20521 gaaaagggtt gagggagaaa aacatatgat caggttgttg gaattaaact atagaaagat
    20581 ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc
    20641 caaacgaatt ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg
    20701 ctggggaacg gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt
    20761 gaatgtacca gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg
    20821 tttattaaat gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat
    20881 ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag
    20941 ctatttatta aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct
    21001 ccagttgcgt atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc
    21061 atttccaatc gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa
    21121 tttgtttcag gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca
    21181 tctcatgaga gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca
    21241 gttgataaat atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa
    21301 gtacagcagc tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc
    21361 ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt
    21421 ttcatccgct gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat
    21481 ttattttttc tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta
    21541 ctttgcacag cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact
    21601 tcaattttat tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt
    21661 ttggatgctt atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat
    21721 tatcagcgac ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct
    21781 tgctgtgcat aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata
    21841 caatcttctc gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc
    21901 tcatatattg attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt
    21961 cgagttatga ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac
    22021 aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc
    22081 gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt
    22141 tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg
    22201 agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata
    22261 gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc
    22321 aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa
    22381 agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac
    22441 tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac
    22501 cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg
    22561 agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga
    22621 aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct
    22681 tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc
    22741 attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc
    22801 gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct
    22861 gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg
    22921 cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta
    22981 agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt
    23041 gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta
    23101 gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga
    23161 agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct
    23221 cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat
    23281 tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa
    23341 ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta
    23401 ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg
    23461 cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca
    23521 tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta
    23581 ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc
    23641 cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa
    23701 taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat
    23761 tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac
    23821 ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg
    23881 gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta
    23941 gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg
    24001 tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag
    24061 ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt
    24121 ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc
    24181 ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca
    24241 gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa
    24301 tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta
    24361 gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat
    24421 atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat
    24481 gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt
    24541 gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct
    24601 taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag
    24661 tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta
    24721 ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat
    24781 ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg
    24841 atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag
    24901 actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa
    24961 cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg
    25021 aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat
    25081 atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa
    25141 ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa
    25201 gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa
    25261 gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa
    25321 accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac
    25381 acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt
    25441 tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct
    25501 gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca
    25561 gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca
    25621 gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa
    25681 tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa
    25741 aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc
    25801 atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc
    25861 ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac
    25921 gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt
    25981 gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga
    26041 gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct
    26101 ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc
    26161 agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc
    26221 tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt
    26281 taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc
    26341 aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga
    26401 taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca
    26461 taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc
    26521 aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata
    26581 cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt
    26641 atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa
    26701 ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg
    26761 cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg
    26821 accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg
    26881 tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt
    26941 tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg
    27001 cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg
    27061 tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt
    27121 ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct
    27181 ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca
    27241 tcatctagat ttggtttgcg atcgatatct tcagtcttgc ttgtacccat tgtatcagtc
    27301 tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt
    27361 tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca
    27421 gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt
    27481 tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta
    27541 tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa
    27601 aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca
    27661 tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca
    27721 gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact
    27781 gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg
    27841 taaattcctc ctagcaaata gatttatgat tggtata
//
LOCUS       DAXWLG010000020        27786 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXWLG010000020 DAXWLG010000000
VERSION     DAXWLG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.020"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            190..1929
                     /gene="yfcC"
                     /locus_tag="ACGE4T_000651"
     CDS             190..1929
                     /gene="yfcC"
                     /locus_tag="ACGE4T_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFF8385958.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2086..3270
                     /gene="iadA"
                     /locus_tag="ACGE4T_000652"
     CDS             2086..3270
                     /gene="iadA"
                     /locus_tag="ACGE4T_000652"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFF8385959.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3381..3584
                     /locus_tag="ACGE4T_000653"
     CDS             3381..3584
                     /locus_tag="ACGE4T_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8385960.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3685..4347)
                     /locus_tag="ACGE4T_000654"
     CDS             complement(3685..4347)
                     /locus_tag="ACGE4T_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385961.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4360..5862)
                     /locus_tag="ACGE4T_000655"
     CDS             complement(4360..5862)
                     /locus_tag="ACGE4T_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8385962.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6410..8089
                     /locus_tag="ACGE4T_000656"
     CDS             6410..8089
                     /locus_tag="ACGE4T_000656"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8385963.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8216..8695
                     /locus_tag="ACGE4T_000657"
     CDS             8216..8695
                     /locus_tag="ACGE4T_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFF8385964.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8914..9594
                     /locus_tag="ACGE4T_000658"
     CDS             8914..9594
                     /locus_tag="ACGE4T_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFF8385965.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9602..10933
                     /locus_tag="ACGE4T_000659"
     CDS             9602..10933
                     /locus_tag="ACGE4T_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFF8385966.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10948..11910
                     /locus_tag="ACGE4T_000660"
     CDS             10948..11910
                     /locus_tag="ACGE4T_000660"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFF8385967.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12021..12359
                     /locus_tag="ACGE4T_000661"
     CDS             12021..12359
                     /locus_tag="ACGE4T_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFF8385968.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12438..13145
                     /locus_tag="ACGE4T_000662"
     CDS             12438..13145
                     /locus_tag="ACGE4T_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFF8385969.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13248..14729)
                     /locus_tag="ACGE4T_000663"
     CDS             complement(13248..14729)
                     /locus_tag="ACGE4T_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385970.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15038..15574
                     /locus_tag="ACGE4T_000664"
     CDS             15038..15574
                     /locus_tag="ACGE4T_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8385971.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15652..17022
                     /locus_tag="ACGE4T_000665"
     CDS             15652..17022
                     /locus_tag="ACGE4T_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFF8385972.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17089..17424
                     /locus_tag="ACGE4T_000666"
     CDS             17089..17424
                     /locus_tag="ACGE4T_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385973.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17505..18362)
                     /locus_tag="ACGE4T_000667"
     CDS             complement(17505..18362)
                     /locus_tag="ACGE4T_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385974.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18469..18984
                     /gene="tadA"
                     /locus_tag="ACGE4T_000668"
     CDS             18469..18984
                     /gene="tadA"
                     /locus_tag="ACGE4T_000668"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFF8385975.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            19007..19091
                     /gene="ffs"
                     /locus_tag="ACGE4T_000669"
     ncRNA           19007..19091
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGE4T_000669"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19301..20686
                     /locus_tag="ACGE4T_000670"
     CDS             19301..20686
                     /locus_tag="ACGE4T_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFF8385976.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20723..21400
                     /locus_tag="ACGE4T_000671"
     CDS             20723..21400
                     /locus_tag="ACGE4T_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFF8385977.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21408..22172
                     /locus_tag="ACGE4T_000672"
     CDS             21408..22172
                     /locus_tag="ACGE4T_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFF8385978.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22174..22833
                     /locus_tag="ACGE4T_000673"
     CDS             22174..22833
                     /locus_tag="ACGE4T_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFF8385979.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22863..23372)
                     /locus_tag="ACGE4T_000674"
     CDS             complement(22863..23372)
                     /locus_tag="ACGE4T_000674"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFF8385980.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23484..24482)
                     /locus_tag="ACGE4T_000675"
     CDS             complement(23484..24482)
                     /locus_tag="ACGE4T_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8385981.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24683..25267
                     /locus_tag="ACGE4T_000676"
     CDS             24683..25267
                     /locus_tag="ACGE4T_000676"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8385982.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25344..26519)
                     /locus_tag="ACGE4T_000677"
     CDS             complement(25344..26519)
                     /locus_tag="ACGE4T_000677"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFF8385983.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26601..26981
                     /locus_tag="ACGE4T_000678"
     CDS             26601..26981
                     /locus_tag="ACGE4T_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFF8385984.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27055..27417
                     /locus_tag="ACGE4T_000679"
     CDS             27055..27417
                     /locus_tag="ACGE4T_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385985.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9040 a         4690 c         5791 g         8265 t
ORIGIN      
        1 agaaataaaa tacttaatat aaatggaaag agaaaaaaat aatcatacat aggagaaaaa
       61 aaacaaaata gaaaaaagtg attttctaat aaattattca tgtggaaatg tcatttttac
      121 tgtataattc atttgattat ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa
      181 gaaataaatt tgaagaaaac aaattcttca ggaaaaccga aacgcgactt cactaaatta
      241 tcgacacccc atacgtatgt cattattttt ggtgtagtca tctttgcatg gatactgact
      301 tttgtcgttc cagcaggaaa gttcagcacg caagatattg aatacaaaga tgcaaatggc
      361 gaaacaagta ctcgtactgt tttacgtcaa gattccttcc gttatgctta cgagctggat
      421 aagtcgtatg tgtttgatca attagaagaa ctgcaagatc atccagcaga acgtgaaaaa
      481 ctggatgttc cggaaaaagg attggaaaaa gtcattgctg acggagaaaa aaatcttacg
      541 caggaaaaac tagatgaaat ctccttgaca gatgatgtgc tttacgatca gtacggcgaa
      601 aatatctatg atacttctaa aaaattgcat aaaacagcca aaatatgggg aacagatgat
      661 tttggcggct ttggtttctt aaattttgtt tttgaaggac tagtatctgg agataagtat
      721 ggatctgctg tagggatcgc tgcattgatc cttgttgttg gaggtgcttt tggtattatt
      781 atgcgtaccg gagcgatcga cgcaggtatc tatgcgttta tcagtaaaac taagggactg
      841 gagcgtttag ctttaccgct attattcttt gctttttcat tcggtggcgc tacattcggg
      901 atggcagaag aggtaattcc attctcaatg gtgatggttc catttgtcat tgcgttagga
      961 tatgactcga tcgtagcagt aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc
     1021 tcttggatga gcccgtttag cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg
     1081 tctggtgcga cattccgatt gattatgtgg gtagttgtta ctgcattagc cgctggttat
     1141 atgatgatat atgcagaaaa aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca
     1201 gatgattatt tccgttctca tatcaaaaag acatctgatg aaaataaaaa attcatgctt
     1261 gggcataaat tgatcttatt agagatgtta gttgtccttg tctggatcgt ttggggcgtg
     1321 actcaaaaag gctattacat tccagaaatc gcttctcaat tctttgtgat gggattagtt
     1381 gctggaatca ttgctgttct tttcaaatta gatggaatga aagtcaatga catcgcttct
     1441 tctttccaat ctggtgcagc tgatttagct ggtacagcaa tcgtagtagg tatggccaaa
     1501 gggattcttc ttgtcttggg aggatctgat gcatctgttc cttcagcttt gaatacgatt
     1561 ttgcatggga tcgggacggc gttgaccggt gttccagctg tcattggtgc ttgggcgatg
     1621 tacatcttcc aaagtttgtt caacttggtt gttacatcaa attcaggaca agcagctttg
     1681 actatgccaa tcatggctcc actggcagat ttagtaggtg tatccagaca agtagcagtt
     1741 cttgcttatc agcttggttc aggttttatg gacgctttta cacccgtatc agccagcttg
     1801 attggtgtgc taggtgttgc acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag
     1861 atgcaaggat tcctgtttgt tttaggaact attttcatca tgattgccat tgcaataggt
     1921 ctgcagtagt actggagacc tgtaaataaa aaagtaatcc aaacagtaga taaggactcc
     1981 aagaaccgtc attttacggt aaaatcaaag gagtaaagat cctatcgttt ggattatttt
     2041 ttaagatcct atcatcagtt atacttaaag aaagaggaaa aatgtatgaa aatcattcga
     2101 caaattgaag tgtttgcacc agattactta ggaaaaatgg atgtactgat tgccggtggg
     2161 aagattttgg ctgtagaaga acagctagaa ggcggctacg aaggagtgga agtagaagaa
     2221 ctttcaggtg agggaaaagt actaaccccg ggtttcattg actgtcattt ccatatttta
     2281 ggtggaggag gagaaggcgg ttatcaaaat cgaacgccag aagtcacatt gagccaattg
     2341 actacagctg gtgtaacgac agttgtcggt tgtttgggaa cagacggaga aggtagagat
     2401 atgactgcgt tgatcagcaa agcgaaaggt ctggaagcag aaggaatctc cacttatgta
     2461 tacgaaggtt cgtaccgatt gccggtaaaa acagtaacag attctatcat caaagacttt
     2521 ttgacaattg ataaaattat cggaatcgga gaaatcgcag tatccgatca ccgttcttct
     2581 cagccaagtt ttgaagagtt cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta
     2641 tccggaaaag ctgggatcat caatgtccac ttaggcggag gaaaaagaaa gctagagcta
     2701 ttgacacgaa tcgtagaaga aacagaaatt ccgatcacac aatttttgcc tactcatgca
     2761 aatcgcacac cagaattatt tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt
     2821 gacttcactg ctagcgaaga tcctgatttt tgggagaaga cagatggtga agttcgcttc
     2881 agcaaagcac tgaaacgatt gatcgaggaa gatgtttctt tagataattt cacgatgacc
     2941 tcagatggac aaggaagctt gccttatttt gatgaaaaca atcatttcct agggttagga
     3001 gtaggaagtg ccaaggcatt gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc
     3061 cctttggaaa tcgccttacg agctataacc agtaatccag cacgtatatt gaaattggat
     3121 aaaaaaggaa aaatcgaaat tggtgctgat gcagatctgt gtatcttgga taaagaaacg
     3181 cttgatattg atacagttat cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa
     3241 gtatggggga ctttcgaaaa aagtttttaa tcaacagctc taactatctt ttttagaaaa
     3301 cgagcgttct gtttgtctgt atctgttgtt gatgttaccc tgaaggtaga tcaataaaca
     3361 aactaagagg aggaagcaca atggcagatt taaaaggacg ttctaaagat atgaaagata
     3421 aagtagtcgg tgaaggaaaa gaagcatacg gcaaagcaac agacgacaag agcaaagaaa
     3481 cagaagggaa agcacaatca ctaggtgctg acatcaaaga aaaagctcga aatttaggcg
     3541 acgaagtcaa agacaagttt tctcgaaaag acgaaaaaaa ataaaaacca cttattacta
     3601 attgaatcaa gcaaataaaa aatccgaacc atatctgcga gacagatatg gttcggattt
     3661 tttatttgat cattatcctg ttttttaaac ttcgattttt tgaaattttt tattttttag
     3721 ttcaaagact tcatcacagc tttttgctac ttcaatctca tgagtcacga tgataataca
     3781 gcgattttgt tcatgggcga tttcttggaa aagtttgacg atatcttgtg aagttgtttc
     3841 atctagattt ccagtaggct catccgctac gattagttga tgttcacagc agatagcacg
     3901 aacgatagcg actcgctgct gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa
     3961 tatttcatca attcctacac gagccagtcg atctaaagca acttgtcgat aattctcttg
     4021 cttggtctta gtgatttcca ttgctgctat gacgttgtct agagccgata aataaggaag
     4081 caagttgtaa gcttggaaaa taatcgagac atcattttta cgataacttc ttaacccgat
     4141 tttgttcagt gaacgatctt gatagtatac ctttccttct tttggtgcat ctagtccaga
     4201 aataagcgaa agaaaagtcg tttttccaga accacttgct cctaaaatag cgtacatttt
     4261 tccagcttcg aaattcagtt ctacatcttt ataaagcgca tcttgcggat cttgatacca
     4321 aaatcccaat tgttctgtcc gtaacatcaa attcctcctt tagtttaata ggatttttct
     4381 tgggtttaat cttaatatcc ctatagaagc taaaaagaca gaaaacagac tgattccaaa
     4441 tgctactccg gctaatagtc ccaattgtgc aggtttgaca ttgatttcca attcgctgac
     4501 ttgatcagaa acagcaaatg gattattcgt ggttccttgt tgaccatcgc gttgaccgcc
     4561 ttgttcttgt ctgttacctg gcatttgtcc accgccagga gcttgctggt tttgtgtcgt
     4621 ggtttctgtc tgttggtcaa gtagctgctg tcccacggca ttcgcgacga cgtttccgct
     4681 aagcgaagca actcctaatg caagaatcat acaaatagcc acttcggtaa acaattggag
     4741 aatgatttta cttctggatt ctcctaaaga tagcaagacg cctaactcat gccgacgctc
     4801 ccgaatggta atcataatga tcagagtaag tatgataata ccggctacag ccactagtaa
     4861 aacaacattt ttagaaaaac ttgccacgtt gtttaaaggt tccaacattg actgatacat
     4921 ggaatcattt gattgtaagc taaatgtatc tgtgtcgatc agcttctcag cttctttgag
     4981 aaactcatcc attttatctg gatcagatag agtgtaaata gcagaatcta tcgtgttatc
     5041 gtcagatgtc ccatttagtt catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg
     5101 attcatgaag ttgaatttca tacccatact gccgcttgtt tcgctggatt cataaattcc
     5161 tttgatcgtc atttcataag tggtatcttc atcttcagta ctcgtgattg tgaaagtatc
     5221 tccgactgcc aggtcatttg tttctgctag agtagaatca atgactacat tgtttgtgcc
     5281 tttgtcgctg tcatctattc cttcaccatc cgtgattttt gctgttccat cagaaaaatt
     5341 actattttgt gcagttgctg acacaccact gatttgaaag tctgcttgag tcatttgcgg
     5401 attcccgccc attcctccag caggttcacc gcctgcatca gtagtttctg aactgtcttc
     5461 acttgaatca gaactagtga tggcagttat tccatctttt gccatagctg aggcggaact
     5521 ttcaaaggaa taactcttta cattatctaa ttcagctatt ttttccgcgt ccgttaagga
     5581 aactggtgta agagaaaaac tgcctggatc tggacgttct tcagtggtag aaccgcttga
     5641 agaagtttgt tcctgttttt taaatgaagc ttctcgattg gcacttaaag tcactgttgc
     5701 tccaacttct ttttgtgctt gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc
     5761 agctagcaca aaaataagaa tggctgtgaa cacggcaaac aataacaatg aacgtcccca
     5821 ttttactttt gtacttttcc atgcacgttt cataaaattc atattttgca tccctcctag
     5881 tagatgcatt tgataataga caacctatct taaagctagc ttaaatctta tgctgtttgc
     5941 acaccatcaa catgaggttc tgtatatata aaaagtcact atagttattg caaaaaatgt
     6001 ggtaaaatga aaaaagtatg tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa
     6061 agaaatccta gatgctacag agggattttt acgtgcatag ttgttctaac aattgctgat
     6121 actagcgatt tagtttattt gttgtataat aatggggaac agctgaatac ttatataagt
     6181 attctggcta tagcgtttcg caaaagatcc attttgttaa tgaagtatat ccaaaataaa
     6241 atgcccatcc aactgtcaga acgcggatgt gctggtaatt ttcctgctga ttctccataa
     6301 gttgagaaat agtgattttg catatgcttc attggaatgg ctcatatatt tagataaaga
     6361 agaaaatcga catctttaaa cttggatgtc ataagaagag gtgaagtttg tgagtaccat
     6421 taaaattatc ccattaggcg gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt
     6481 gggcgaggat atttttattt tagattgtgg gctaaaatat cctgaaaatg agttgttggg
     6541 gatcgacgtc gtaatccctg attttacgta tttagaagaa aacattgatc gtgtagctgg
     6601 tgtcttttta acacatggac atgcagatgc gatcggggca cttccttact tgttgtcaaa
     6661 aatttctgta ccagtattcg ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag
     6721 tcgtaatgaa gcagccaaag gatttaagga tttccatgtg gtagatgagc atacagaaat
     6781 agactttggt gaaacagtag tgagcttttt ccgtaccact catacgatac cagattcagt
     6841 ggggatcaat ctgaaaacaa aagaaggtag tatcgtttat acgggagatt ttaaatttga
     6901 ccaaacggct atcccaatgt atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga
     6961 aggagtctta gccttactaa gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc
     7021 tgaagcccaa atagcagatg aagtatttga tacgatcaaa tattgggaag ggcgtatcat
     7081 cgtcgcttgt gtagcaagta atttgcaacg agtgcaacaa gtattggatg cagcagaaaa
     7141 agcacaacga aaagttgtct tgactggtca agatttcgaa cgaattattc gcacagcgat
     7201 gcgtttagaa aaacttcaat taccaagtga agatctgctt gttacgttga aagacatgaa
     7261 aaactatgat ccagaagaac tgatcatttt agaaactggc cgaatgggtg aacccatcaa
     7321 gtctctgcaa aaaatggcaa acggtacaca ccgtaatgtc aagattgaag aaggagattt
     7381 ggtttatatc actactacgc caagcatcgc gatggaaacc atcgtggcga aaacggaaga
     7441 tatcatttat cgagctggtg gaacagtgaa gttgatctct gagaatatgc gcgtttctgg
     7501 ccatgccaat cctaatgact tgcaattaat gatcaatttg atgaaaccaa catattttgt
     7561 gccagttcaa ggagagtatc gtgaattagc tgctcatgca gatttggctc atgcagtagg
     7621 gatgccatac aaaaatatct atattactgg acgcggagac gtgctggaat ataagaacaa
     7681 gcgtatgagt gttgcaggaa ctacaagtgc agaaaatgta atgattgatg gattaggcat
     7741 aggtgatatc ggaaacattg ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt
     7801 cgtagcggtt gtcactatca gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac
     7861 ttcacgggga ttcgtttacg taaaagctag ccgtgatctg atgaaagaaa gcagcaatat
     7921 gattgaaaca atcgttgaga aacatttaga aagcaatgat tttgagtgga gcaaattaaa
     7981 gcaagacatc cgagatcaat taggccgtta cttatttgaa caaacaaaac gccgcccagt
     8041 gatcttgcca gtcattatgg aagctactca acgtcgaaat aaaaaataaa caaaaagagt
     8101 aagatgaacc tctctataga gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt
     8161 ttgaatacag catccctcgc tttataataa acgatagaat aaagggagtg aatcaatgat
     8221 gagtcaaaca aaagtcgaac gttatttaga agaacaaaag atcagctatc aaccacttga
     8281 ttttagtcat gtggtagaag aatctttcac agaagaacta caaaaaagag ggattgatcc
     8341 agctttgata tgtaaaactc tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt
     8401 gattcctttg aacgatcact tagattacaa aaaaacgaga caagtgacga aagatcgtaa
     8461 agttggtttt cctggaatgg attttgtatt aatgcataca ggatatcccc acggagcaaa
     8521 cacccctatt ggaataaaac tcgcacatcc tgattattgt tttttagcag acagttcttt
     8581 aaaagaaaaa gaagaagtaa ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa
     8641 agtagccgat ttagaaaaga tcgtccatcc tatttacgct gatttaatta gttaagggac
     8701 gagaagagaa gctaagcaga gcagagacct caaacttgag attatatctg cttggtactt
     8761 ggtgcatcct gtattatctg ttacaattgc tttgtacata ctgtactatc aatccaatga
     8821 aaatatattt agcagctata ttttttcatt gatgtgcaag tgatgaaatc gtttttaata
     8881 aaaaagatac agagaaaacg cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc
     8941 tgtttaacaa ttgaaatgga aaatggccaa cgcgtgatga ttgacccgtt tattacagga
     9001 aatccattga cagacttaac tgcagaagaa gtaaagacag attggttgct gattactcat
     9061 ggtcatagtg accacatagg agatatgatt cctattgcaa aagaaaatca agccacaatt
     9121 atcagtattg ttgagattgc tgattatgcg aacagcagag gagtagattc attcggtatg
     9181 aatatcggtg gaaaacatgc gtttccattc ggcgtggtaa aattcgtaca tgcacagcat
     9241 agttcaagct atgaagtaga cggtatcgtt cagtatatgg gcgagcctag cggaattatt
     9301 attcaagcag aaggaaaaac gatttatcat gcaggggata ctgcttattt cagtgatcta
     9361 ggattgcttg cagaagaatt cgatattgat gtcgctttct tgccgattgg cgataactat
     9421 acgatggggc cagaagatgc agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt
     9481 ccaatccatt acaatacttt ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta
     9541 ctggaagata gtgaaggaaa aataatgagc gtctcggaaa cgctggaagt gtaggcagaa
     9601 aatggcgaca aaacatgatt tgattttaga atatattgaa agtttacctg ttggcaatcg
     9661 aatttctgta agaagcatcg caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc
     9721 aatcaaagat gcagaaaatg tcggactagt ttcaacaatt caacgagtag ggaccatccg
     9781 tatcgaacgc aaattaaaaa aacatattga acgtttgaca tttggcgagg tcgtcaggat
     9841 cattgaaggg gacgttttag gcggggcggc aggattagac aaggtcttga ataaattcgt
     9901 catcggcgcg atgactaaac atgccatgga acgctatatt acgccaggat cactaatgat
     9961 cgtcggcaac cgggcggaag tccaaaaatt agcattggaa gatggcgccg cagtattgat
    10021 cacaggagga ttcaatacga ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc
    10081 gatcttacgc acctcttatg atacatttac agtcgcaacc atgatcaacc gagcgttaag
    10141 cgaccaattg atcaaaaaag atatcatgct tgtcagtgat atctatatgc cagctgaaaa
    10201 gactcattat ttgcagcaga cagatacgat ttctgattat cagcgtttgt cagaagaaac
    10261 gcagcattcg cgttttccag tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc
    10321 aaaagatgtt ttagggaaat cgccaaacca gatcatcgat cgggtgatga cgaaagaacc
    10381 aatcagtgta aagaaaacaa tgagtattgc ctctgttagc catcaaatga tctgggatgg
    10441 gctagaagtc atgccggtcg tttccgatga cttgacattg gaaggcttga ttacccggca
    10501 agatgtaatg aaagcgatgc agcttgtcca gcgacaaccg cagattgcag atacgatttc
    10561 ggatcagata tctggaaata tccatacgat tgatactgac cgtgaaggaa atcgtttgga
    10621 aaaccctaag ttcaaattcg tagtagcacc acaaatggtc aatggcgttg gaaccatttc
    10681 ttttggcgta cttagcgaaa tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca
    10741 aaaacgaaat atcttgattg aacaagtgaa cctacattat ttgcggctga tccaattgga
    10801 aagcgaatta gacattcgtc cacgtgtatt ggaaatcggc agacgttccg ctaaactgga
    10861 tatcgaagtg tttattgaaa acaccatcgt ttccaaagca atcgttgttt gtcaagtaat
    10921 ggaacgttct tagaaagcag gagtgaaatg acagtacagc aagaaatagt agatacaata
    10981 aaaaaatacg ataaaatcat tatccacagg catttgcgcc ctgatcctga tgcattagga
    11041 tcacaagtag gattagccga actattacgg gaaagtttcc cagaaaaaga aatacgtcaa
    11101 gtaggtgaaa cgattgaagg gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc
    11161 ttctaccaag gagcgctagt catcgtgact gatacagcta attcacccag aatcagtgat
    11221 caaagatatc agttggcaga aaaactgatc aaaatcgacc atcatcccaa tgatgaccct
    11281 tatggagatc ttgtgtgggt aaatacaaaa gcaagtagct gtagtgagat gattgcagaa
    11341 tttgctttga tgtttcctga atctttaaag atgaacgtta aggctgcacg gttactttat
    11401 gcaggaatcg tcggagatac agggcgcttt ttgtatccct ccacgacgtc ccgtacatta
    11461 gagattgctt cgatattgag aaattatcca tttgatgcaa gtgctttgaa cagagagatt
    11521 gaacaaatgc cgatgaaagt cgctaaactt tccggttatc tttatcaaaa tatccaggtg
    11581 gatgaaaatg gtgcaggaag aataatcctt ccgcaagaaa ttcttgataa atatggaatc
    11641 gatgattcag agacagcttc gatcgtttca cttcctggag tgattgacga agtgctggct
    11701 tgggggattt ttgttcagca gccttcaggg tattaccgcg tacgtttacg ttctaagggg
    11761 ccgatcatca atgaattggc taaacgccat catggaggag gccatccatt agcaagtggt
    11821 gcgaatgcaa aagatttagc agaagtagag gaaatctata aagagatcca agatatatgc
    11881 caatcgtata agagcagaaa aacagaatga tacttcgttc gaaaacgtgc cttttgtagt
    11941 aaaaatcaca aatgtatctt ttcaagatct tctttttccg ttacaatagt gctcactaaa
    12001 gattttaaag gagaaagaag atgacattac caaattgccc agaatgtggc tcagagtaca
    12061 catatgaaga tcgaggcttg ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag
    12121 aacaagaaac tgtcatagaa gacggattag tggtattaga tgcaaatgga aaccgtctgt
    12181 tagagggcga tagcgtgaca gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca
    12241 ttaaacaagg aacaaaagtc aagaacatcc gtttagtaga aggcgatcat aatatcgatt
    12301 gcaaggtaga aggatttgga ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa
    12361 gaatattcaa ggtcttttca cattcttttc ctactactaa tgataaaata aagtaaacga
    12421 aaaggagtga aacgaaaatg tatggttatg gaatgtatgg ttttttagat ccgacatttc
    12481 ttttggtcat tcttggatta gttatttccg gaattgcgtc agcgtacgtc aatagtactt
    12541 tccgaaaata cgatcaagtg agaagcaaaa acaatgtgac tggtacacaa gcagcacaat
    12601 atatattaca aagtcaacag ataaataatg tcggtgttca acaaattgcc ggagatctca
    12661 cagataacta caattcaggt aataagatgt tgagtttgtc tcaagcaact gcacagtcta
    12721 cttcagttgc tgcgataggt gtagcagctc atgaatgcgg acatgcggta caagatgctt
    12781 cgggttatgt cccattgaag attcgtgcag cacttgttcc agcagctaac tttggttcga
    12841 tgatttcgtt ccccttgatt atggtaggtg tattgcttag ctggaactct acgttaatca
    12901 atatcgggat tttcgctttt tctttagcat tacttttcca attggtgaca ttaccagtcg
    12961 aatttaacgc gtctcggcga gcaatccaaa ttttgagtga aggcggacta ctgacggaag
    13021 aagaagtacc gatggcaaga cacgtattat ttgcagcagc actgacttat gttgcagcag
    13081 cgctttcgac attcctgcag ctgttgcgct tgatcttatt gttcggcggc aaccgaagag
    13141 attaaaataa agaagttgtg aaatcagcgc tttgatctga gctaataccg agagttagct
    13201 gattgaatac catttatttg gaataagagg atgtgacaag aattttgtca catcctcttt
    13261 gttttgttct cataaccttt tttcatatac agttcatgaa ccgcttttat taacttgcca
    13321 aataacactg tattgtgcgt tgtatccaga tagaaagtct ccgtttcatc tgaaattttc
    13381 tcatgctgat ctgtaatgat caaatcagca tgttctgaat atgcggtaat tttaacagca
    13441 tctttgcgaa ataactgctg caaatggctt tgaagatgat aatttcctgt aaactgtttg
    13501 gttatctgca aatggatgag taactgctct ttcgtttctg tttgtagtaa gtgttggatc
    13561 aattgactaa ggtattgact gatttggttt ctttccccat tttttgaaaa aattgtatcg
    13621 attttttctc ttaatttctt ttcctgtata aaagaactag aatgattgga cgtggcttct
    13681 ggaaagcaaa gtgtagaaaa actactgctg gattcaccca acagttcgaa aaaaacaact
    13741 gccaacacaa gaaaataaaa ataaattggt attttatttt ctaaatggtt gagaggaaac
    13801 tgttgagtta agtaagtcat caattgaccg acttgtcgac aaataaggtt tttgcttgtg
    13861 gaaaataaaa aaccaatctc ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa
    13921 tagaaaaaat aattaaagta ggctctctct gtttctgtct cttcacaagg gcgactcact
    13981 gaaaaagaag taaagtattc tgaataatca tgtgtatgct gcaataagtc catcaattca
    14041 gtgatttcag aagaagtttt tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa
    14101 gttaacagca agtagatatt tttttcttgt tttgaaattt tttctatttg ctcatctgta
    14161 ctgttcattt ttagtgcatt taattcaagt gaggaaaatg gccaatcgtt acctccaaat
    14221 gtatctgaaa gaaaaaagta aagatagaga cggatagata actcatttcc ttcaagagta
    14281 cagcaatttt ttacagatag gatccgtaaa tggaattttt ttaaggaatg attgagctgt
    14341 tttgtcagtt tttcgagata ggagggcgat atataaagtt gttcaagtaa atattttttt
    14401 ggtacagcag tttgtggata attgattagt aacatcaaat agttaaaacg attggattct
    14461 tttaggtagc ataattttaa cttagaaaaa acagctaact ttttagtttg atcggtttct
    14521 ttggtttgga tctctccttt atgattagaa taaataaatt catcaatcaa taaaaattgc
    14581 ttgagtgata catttatttt tcggatcagt tctttgattg tccgttttgg aatatgtaag
    14641 tcattggata attcttgtaa agaacaatta tttttttgaa tgaccatttg tagaacaagt
    14701 aactcttttt ttcttgcttc aattagcata aatacctcct ttttattctg tttttgtata
    14761 tagttagaca aaaactgtga gtggaaagtc atttcagtct gtgttattct cacagagtgt
    14821 cgccttcttt tttatataac gttatttaat ttttttgtta ccttgtttat tctattgttt
    14881 atttcctagg gtattttgtc aagatattct tgtttttaag gcggaaaaat tgatttttaa
    14941 tggatataac taaatatgtt ttgataatta tcaataatat ttttagaatt atttatgtta
    15001 tttatttatg ggtagatgaa aagagacgga ggtaagaatg atggggagag taacaaatta
    15061 tagtaaagaa tatttgctgt tcaaaagtat ggtatacgtg gaagaatatg gaatgaagtc
    15121 aattactgca agagagttag cagagttctg cggttgttct acatacccga tttatacaca
    15181 tttcaaatca atcagcgggt taaaagagaa gatattagaa gaaatcactg tttattttga
    15241 aaactactta gctgaatgca gttccgatga tgttttaagc attgtctact tattgaaaga
    15301 ttttttttct atccatgaaa gtattcgtga aatcattgca aaatctgatt tagacatgga
    15361 ttcgctattt aagcggccat ttttctcata tgtcaaaggt cacacgaaga tcaaagaaga
    15421 aaagtcgctt gaattggtat ggcttaatgc attgggaagt ttgcgctcag atgattcttc
    15481 tcaagatttt cttgaaatga tgaacgctgt ttgggataaa aacgaggtga cggatgaata
    15541 cttggaaaat atcttaaagg aacagtcacc ttaggcagca acatgaaaaa gtagatcagc
    15601 cacctttatt tttcgtaaaa aagattgtaa aatgtctaac gaaacattgg aatgaaggag
    15661 gaagcgacaa cgttaccaca gcaatttatc aaaaaatatc gtcttctttt aggcgaagaa
    15721 gcatccgatt ttttttctgc gcttgaacaa ggaagcgtga aaaaaggatt tcggtggaat
    15781 ccactaaaac cggcaggatt ggacatggtc caaacttacc atagcgaaga attacaaccc
    15841 gctccctaca gcaatgaggg atttcttggc actgtcaatg gaaagtcttt cctccatcaa
    15901 gctgggtatg aatatagcca agaaccaagt gcaatgatcg ttggaacagc tgctgcagca
    15961 aaacccggtg aaaaagtttt ggatttgtgt gcggctcccg gtggtaaatc aacccaatta
    16021 gctgctcaaa tgaaggggaa agggttatta gtgacgaatg agatctttcc gaaacgtgcg
    16081 aagattttat cagaaaatat agaacgatgg ggagtttcga acgcaatagt gacaaatcat
    16141 gcacccgcag aacttgtccc tcatttttcg ggcttttttg atcggatcgt ggttgatgct
    16201 ccttgttcag gagaaggaat gttccgaaaa gatccaaatg caataaaaga gtggacagaa
    16261 gaatctccgc tatactgtca aaaacgtcag caagagattt tgtcttcagc aataaaaatg
    16321 ctaaaaaata aaggacaact gatttattcg acttgtacgt ttgcaccaga agaaaatgaa
    16381 gagatcatta gctggcttgt agaaaattat ccagtaacga ttgaggagat tcctttgact
    16441 caatccgtta gttcaggacg ttctgaatgg ggaagcgtag ctggtttgga aaaaacaata
    16501 cgtatatggc ctcataaaga tcagggtgaa ggacattttg ttgcaaagct gacttttcat
    16561 ggacaaaatc agatgcataa agaaaagaag acacgaaaaa aaagtaaagt gcaaatgaca
    16621 aaagagcagg aaaaactctg gacggagttt tcaaatgatt ttcattatga agctactgga
    16681 agacttttag tgttcaacga ccatctttgg gaagttcctg agttagcccc ttctctcgat
    16741 ggtttaaaag tcgtacgtac aggtttgcat ttaggagatt tcaaaaagaa tcgatttgaa
    16801 cctagctatg cattagcgct tgctacaaaa aagatagaga atataccttg cttgccgatc
    16861 actcaaaagg aatggcaatc ttacacagca ggagaaacat ttcaacgtga tgggaatcaa
    16921 ggatgggttt tgctagtatt agataagata cctgtcggat ttgggaaaca agtaaaagga
    16981 acagtaaaga actttttccc taaaggattg agatttcact gagtggatta gttcgtttat
    17041 ttttttgtct atgctatgat ggagatagat aaaaaggagc tgatacttat gaaaacatca
    17101 acaaaagtaa caatcggatt aagcgtagca gccgctgcca gtgtagcgac agcagtcgtt
    17161 gtatcgggaa aagtaatgga aaagatccat catatgacta ctcggacgaa agtaaaaaaa
    17221 ttcgtctatg acaaatttga tggaaatgaa aaattactag aaatcgtaga tggtctttca
    17281 gatagtgact tggattcttt gatgggaatg ttagcaaaaa tcaaatcagg aaaaaagaaa
    17341 atctctgttt atggtgattc aatcaaagat tcaaccgaag atgtaaaaga ccggctaatg
    17401 cattttgttg aaaaaatgat gtaagagaaa ctagcttttg aacactattc attcgaaata
    17461 aggggttgtg acaaaattat tgtcacaacc cctttaatct atctttaatt atgttcatga
    17521 agcaataaaa gatagcaaaa cagtaaatcg ttggcttttt ttagattgaa accagactgt
    17581 tcttgaaatt tttcgattcg atattttaga gtattacgat gaagaaacag tgtttttgca
    17641 gcagagctga cattgcctaa ttcattccat aattcttgaa tgatcacttc catttcattt
    17701 tgtttgatca gttctatgta acttttgatc aacttactat cttttacagt atcttttgta
    17761 tagtaatgta aagccacatc ttgcatagaa aaagtacggc tgtgagtata aagattgttt
    17821 tgctctgtta aaaaaatttt tctttcttca gcaaacaaag ggacgaggtc ggtgccaggt
    17881 gaatggaaag aaccaacgaa tacagacgtt gtcgtatcaa aatcagcatc taaagataga
    17941 aaaataccgt tgatttcagt agcatcaaga taatcacggt ttatttgttc gactaagagc
    18001 gcatctgtgt ctgtataaaa aaagaaatca gcagaatagc gaaataattc caaaatagta
    18061 tcttgccatt ctttttttaa gaattctcct tttgattcga cgtgaagctg gatcaaacga
    18121 aagctttttt cttgaacaca agaagcatct ttaaaaagat agtgatacca tgggtgtttt
    18181 ttttgatcgt tgattacagg gaataacgct ttcaaaaggc tggtttcttg ttgagataat
    18241 gaatcttgtt tgatccagat aaaagcatca tcaattgcaa aagaaaggat atccgcttct
    18301 gatgctggag agtttttttt ctgtgcttgt gggtaaagag caagtaattt atccattttc
    18361 atcttgttca cctcttgtat ttattttacc atagaaaaaa aagttagtta cagccttttt
    18421 atcatatgct aaagtaaaac aagaaaaaac aaaggcaggg aagggtaatt ggtaaataca
    18481 aatcttgatg cagtagaaaa agaaaaatgg atgcgccttg cattggcaga agcaaagaaa
    18541 gcagaaatgc ttcatgaagt tccgattgga gcagtggttg tattggatgg aaaagtgatt
    18601 ggaagaggat ataaccttag agaaaccacg caagatgcta ctacacatgc agaaatgctt
    18661 gcaatcaagg aagcctgtga aaaagtggga agctggcgct tggaagatgc tgcacttttt
    18721 gttaccttag agccttgtcc tatgtgcagc ggcgcgatga ttttgtctcg tgtagcagaa
    18781 gtttatttcg gcgcgtatga tccaaaaggc ggaacagccg gtaccttgat gaatcttttg
    18841 gaggacgagc ggtttaacca ccaagcatat gtagaaggtg gcattttaga agacgaatgt
    18901 gggatgattt tgactgactt tttccgaaag ctccgccagc gaaaaaagaa attaaaaaaa
    18961 gactggtcaa cgaatgaaaa ataaggtata ctattctttg ccgaaaggct aggacaatgg
    19021 tgggcttgtg aagcgtgtca ggtctggaag gaagcagcac taagcaggtt ccgccatgtg
    19081 tctgattata atgaaacgac aacagacttt cgggtctgtt ttttttattt ttatagtact
    19141 gtaaaaatga atgttcctga ttttttagcc agactgattt atcgaatatt gtacttatac
    19201 aatttgcttc aaggcaggaa aaactctgaa atagagcttg tttcaatgga agaaaaagct
    19261 gcctatggta tgataaagat aaagagaggc gagagggtac atggaaaaaa tagtgatttt
    19321 acatacgaat gatctgcatt ctcatttaga aaattggcca aggatcagaa ggtttttgga
    19381 tcaacgtaaa cgagaaaacg aaaagaaaga aaaaacgaca acgattgcag tagatctagg
    19441 tgattttgtg gatcgttggc atccattgtc agaagcaacg aatggacagg aaaatatctt
    19501 gctgatgaat gaagttggtt atgatgcagc gacgatagga aacaatgaag gcgtaggaaa
    19561 ttccaaagaa gagttgaatc atttatacga tcatgctgag tttgatatca tcctggataa
    19621 cttattcgat aaaaatacgc tccagccacc ggcatggacg aaaccatata aaatcattac
    19681 aacgaaacac cagacgaaaa tcggactaat tgcatttact gctccatttc cgcttactta
    19741 taatccgaat ggctgggaca tacggaatcc ttatgatatt ttgccagaat tagtcgaaga
    19801 tttacgttca caagtggatg ttttagtttt gatgagtcat ctcgggattc aggatgattt
    19861 ccaaattgca aaagaaatac cagcaattga tgttatttta ggatcgcaca cccatcattt
    19921 attcagagaa ggtcacgtaa tcaatgatgt ccaaatagca gccgctggaa agtacgggca
    19981 gtatgtcggt gaagtccatt taaccatcga tgaagagaaa aaaatcctta agcattcagc
    20041 caaagccatc cctaccgaaa caatgacagc ttttacagaa gatgaacaag aaataaatag
    20101 ttatctagaa cgtgggcatg aattattaaa agaaaaagaa gtagcagatt tgccattttc
    20161 tctgagtcta gatatcttca atgagcattc gtttatccaa gtagcctttg aggcggtgaa
    20221 agaacgagga aagacggaag cagcaatatt aaacagtggt ctgtttttaa ctgaaattcc
    20281 aaaagggcgg gtcaatcagg atcagcttca cactgcgttg ccgcatccga tgcacttgtt
    20341 aaatgtcaca ttgggtggaa atgatctgat ccgcttagtt ttagaaatag aaaaaaatcg
    20401 taacttttta cgtaactatc ctatgaaggg aatgggattt cgcggtaaga tttttgggca
    20461 gatggtctat aatggaatca catatgattc tattaaccat caagtctttt ggaagaacaa
    20521 gccaatcgat ctgaataaaa attatacttt tactaccgta gatcatttga tgtttgttcc
    20581 attttttcca acgattgaga ttgctggaaa aaatgaattt ttgttccctg aatttatccg
    20641 aagtgtggta ggagattact tgaaagctca ctacccaatc aaataaaaac aaggtataat
    20701 aaaaaagata ggtggtgaac gcatgtctgc aaaacaaaat gaacagcaga acgttgagca
    20761 taaaacagaa attgctaaga aaaaatcatc agctgaacag gaaacaacag tcaccgaaga
    20821 actgacagct gctttggaag aaatcgttga ggagcctaaa gcagaaaaag tcaaaggcga
    20881 gctagtgaca ctgttagatg acagtaattt tttgataggt gaaagacatt atcggcttgt
    20941 gtccgattat cgtgaaggat ttaatgcaga aaagcttgga gaacgttaca gtgatgtact
    21001 agcccgatat gattatatcg taggagattg gggttatgaa caactacgcc taaaaggatt
    21061 tttccaagcg gataatcgac gggcgcatcc agatcagcga atcgattcct tggaagacta
    21121 tctttacgaa tattgcaatt ttggttgtgc gtattttgtc atcgagagaa tcggcggtaa
    21181 aaaagaaaag acaactcagc gccgaaaaaa gaaaaaaaac cataatcgtg cgtatatcga
    21241 tgagaaaaaa ggaccagtcg catctaatcg caaaaaaccg gttattaaaa atcgaaaaac
    21301 agagtcagca aaaacctctg aaaaagtaaa aaaacaagaa caacctaaac aagtagaaaa
    21361 aacaaaaaca gcatttacga tcagaaaaag ggaagagtag tggatgaatg gactataaag
    21421 gatatttaat tgatttagac ggaacgattt atcgtgggac acaacccata ccggccggta
    21481 aacgatttgt ggaagaattg caaaaaagaa aattaccttt cttgtttgtt accaataaca
    21541 cgactaagtc accagaaaca gtagctaatc gtctagcaga tgagtttgac attcatgtag
    21601 ctccagagac agtctacaca gctactttgg caacaatcga ttttatgaaa gcagatggaa
    21661 aagggaaaaa agtctatgtt atcggagaag caggattgat tgacttgatt ttagctgccg
    21721 gatttacatg ggaagaagag gctcctgact atgtagtggt aggcttagat aattatctga
    21781 cctatgaaaa agtagttaaa gcaacacttg ctattcaaaa aggtgctact ttcattggaa
    21841 cgaacccaga taagaatata cctactgaac gcggccttct tcctggagca gggtcagtca
    21901 tctcttttgt agagacagca acccaaacgc cgccgatcta catcggaaaa ccagaagcca
    21961 tcattatgga taaagccgtt gaagtattag ggctgcaaaa agaagaagta atcatggttg
    22021 gagataacta tgaaacagat atccaagctg ggatacgcaa taatatagat actttgctcg
    22081 ttttatcagg ttttacaaaa gaagaagatg tacctgggtt gcctatccca gctacctatg
    22141 tgaaacaatc actggatgaa tggagcttct aatatgaaaa attcgagatg gcagtggatg
    22201 gaatatgctg gattgttcag cctatttctg acactgatca gtctggcagt tggtgtgacg
    22261 atcaactttc gaccactata cgtatttgat atcggacatt tgcaaatttt ggattatact
    22321 tctttagatc aagagacgtt actgaaaaat tttgatcacc taatgaatta tctcaataat
    22381 cccttcaaaa ccatcctcag cttgccagat ttcccagttt ctgctagcgg agcccatcac
    22441 ttttatgaag tgaaaatttt gtttttagtg gattatgctg tgtttttcat cactttgatc
    22501 ccgagtattt tgtttatcaa atatttgcaa aagaatgatc ggctttggcg gttgattcgt
    22561 ccgtttcaaa tcggcatgtt actgccagtt gttttcggtt ttttcatgat gataggattt
    22621 gatcgctttt ttatcctttt tcatgaaacg tttttcaaca acgatgactg gttattcgat
    22681 cccgttactg atccaatcat caatgtactt ccagaagaat ttttcatgca tagctttatc
    22741 ttattttttg tattattaga attatttttt gctgtttttc ttttcttagg aaaaaatagc
    22801 ttgaaacaaa caaaaaagaa agaactcgtc taaatagacg ttttctttct ttttttgttg
    22861 aattattgag taaggcgact tcttttttct ggttctttgt gtgctaatcg actggcagca
    22921 gcagcggcga tcgctccgac gatatcatct aagaaggtat ggacatgaac gccatcgtgg
    22981 ctgtttagtt cttttaaaat accaggtttc accttgtcga tataaccata attcgtaaag
    23041 ccgatcgtac cgtaaacatt cacgattgac aaggctagga tctcatcgat accgtaaagg
    23101 ccttcgtcat cttcgataat atgttgcaac ggactcatca attttttctt ttctgctaat
    23161 atatctaatt ggattccagt gataatggcg ttatgtactt cacgctttgt tagcacgctg
    23221 ttgacacttt cagtacaatc cgccaaagtc aaatcagaaa tatagtcttt ttgtaaaaac
    23281 atgacaagtt gtgcgatatc ttctacagtt acgccacgtt ccttcaatag ttctcttgcc
    23341 ttttcttcta aagtctcagt tttgacgacc atattattgc tcccccttta ttttttcttt
    23401 ctctatttca tgttaacgga acatgaaaaa aatgcaagaa actgctttcg ggtttaactt
    23461 gaaatgcaaa gagatataaa aaatcaacta ggacgaagat ttttatcgta taagcttgtg
    23521 ctgtgaccag gctgagttcg ttcctttgga ttaatatagt gtaaagcgtt gttcactgca
    23581 gtaggtgctt caccgagccc agtagcaatc agtttcactt ttccttcata ggtacagatg
    23641 tcaccagctg cataaacgcc tggaatagaa gtagacatat ctgattttac agtaatggca
    23701 tttctggtac tatcaagtcc ccaagaagaa agatgttcta aattagaaga gaatccataa
    23761 ttgacgatca gagaatcaat catcaaatca atcgtctcgt cacttttcac tttttttaac
    23821 cggatatctg tcagttcccc attattgcct gataatccat cgatgagata gggagtaagc
    23881 aagttgacag atgaagattt caagcgtgat acactatgtt cgtgcgcacg aaattccggg
    23941 cgacgatgaa tcaagtaaac ttcgcttgca ataggctcta acatcaacgt ccaatcgata
    24001 gcagaatcac cacctccagc aatagcgact ttttttccag catacttcat caaatcattc
    24061 acgaaataat ccaatccgtg gttttcaaat gattcagcat tatctaaatt caattttctt
    24121 ggctggaatg aaccatttcc taaagctaaa atgactgctt tggaataatg gatgccttta
    24181 tttgtagtaa tctcgataac ttcgtcttca cgagtaagcg agagaacttc ttcttttaaa
    24241 tggaatgtat ggttaaaagt agttaattgt ttttctaatt gatcgattaa ttcacttgct
    24301 tttattgctg gatacccagg aatatcgtaa atatattttt cgggatacaa agttgctaat
    24361 tgaccaccaa gttgaggtag actgtcaatg atttttgttt tagcttggcg cattcctgcg
    24421 taaaaagcgg caaacatccc tacaggacct gcaccgatga ttgtaatgtc atagatttcc
    24481 ataaaaattc ctcccttttc acactatagc agaatttaat tgagaatgct tctcaaaaat
    24541 gtttgaattc gaaatcgaaa cggtaagtta tagaatgtat ctagtgatga aaaataaaag
    24601 aaaacaaatc tagtattttt acgagaggtt aggtattatg ttattattat tatgaagtat
    24661 gcaaatatta ggaggaaaca atatggcatt cccacaacta gatttaaaaa gtgaaaaagg
    24721 accaaaagct gttatcaaaa caaaccgtgg tgacattact gttcaattat tccctgaact
    24781 agcaccgaag acggttcaaa atttcattga actttccaaa aaaggttatt atgatggcgt
    24841 gatttttcac cgcgttattc ctgatttcat gatccaaggc ggcgatccaa ctggaacagg
    24901 aatgggtggc gaaagtattt atggcgaaag ttttgaagat gaattcagtc gtgaactgtt
    24961 caaccttcgc ggagctttat ctatggcaaa ttcaggtcca aacacaaacg gaagccaatt
    25021 ctttattgta aataacacaa atgtgccagc taatatgctt ggacaactag aaggtgctgg
    25081 attcccatca gaaatcattg aagcgtataa aggtggcgga actccttggc tagatttccg
    25141 acatacagtc tttggacatg tactagaagg gatggacaca gtagatgaaa tcgcaaatgt
    25201 acaacgtggt ccacaagacc gacctgtaca tgatgtagtg attgaaacaa ttgaaatttc
    25261 agaatagttt ggtatggtac ggagtaatta tgaaaaagga cgaaacctag taaggtctcg
    25321 tccttttatt ttgaataaat gatttaatcg attgtctcta aagcatactt gattcgatta
    25381 agtccttcca gtaaagtttc ttctggacaa gcaatattca agcgcatatg cccatgaccg
    25441 gaaggaccaa acgtgatgcc aggatttaat acgacttttc ctttccgaac aagagtgttc
    25501 tccaattctt ggtcgtctga aatataggcg gtaaaatcta accacattaa gtaagttcct
    25561 tcagggttca ttattctcac tttcggaaga tactgttcaa aaaactgatt aactgtttga
    25621 acattttttt ctagataagg aatcagttgg cttaaccatt ctccacccgt ctcataagcc
    25681 gcttgggttc caatcagtcc aaaagtgttg atttcgtgct gttggttcat cactaaatgt
    25741 ttttcaaaat cagctttcat ctctggattt ttgataaata ccattgaatt tttgattgca
    25801 gctagattga aagtcttggt tgcggaagta aaagcaacta gtagatcatc taaattagga
    25861 tcaatcgtta gcatggaagt aaatgtatga tcgaataaag ttaaatcttg atggatctcg
    25921 tcactaaaca gaatcacttg atgttttaaa cacaattcgc ttaactgttt caattcttct
    25981 ttgctccaaa cacgccctcc aggattgtga ggattgcata aaatcattgc ttttacattg
    26041 ttttcttcga tttttttctc catgtcagac aagtccatga tgaaatgatt atttttttct
    26101 agcaaagagc ttcgaacgat gtgacgttga tttgtttcta taatagagga aaatggagga
    26161 taaactgggt catgaatcaa aatagaatcg cctggttttg taaaagtttg tactgctgca
    26221 gctaaactgg ttaacacacc acttgtaaaa acgatatttt ctttcgtcaa ctccacagca
    26281 tggtgttggc gttcccaatg aatgatagcc tgatatagtt tatcagacag agtggcataa
    26341 ccaaagattc catgtttgat gtattcacta aacgctttag ctaccccacc gggagacaag
    26401 aagtccatat ctgctaccca caaaggaagt aagtcatcca tttgatatgt ctcagcaatt
    26461 gcgtcccatt ttacactttc tgtatctttt cttgaaatct gtttatcaaa ttcgatcatc
    26521 ttaagacctc cagtcatttt cgtcgcttct ttttttgagc tatttacagt ataatgataa
    26581 atgcgaagag agggaagtag atgacttata aaattggaca aataattgaa ggaaaagtga
    26641 cagggataca gccttatggc gcattcgttt ctcttgataa cgagacgcaa ggacttattc
    26701 atgtttcaga agtacaatcc ggttatacga aaaatatcca ttcattgtta aaagtcggac
    26761 agccagtaac agttcagatc atcgacattg atgaatattc caaaaaaatc agtttatctt
    26821 taagaacgtt agaaaaaaca aatccagctg ttccatatcg cagaaaaagg tatttcacga
    26881 ataaaaatcg taagatcgga tttaccacta ttgcggagca gttacctatt tggatagaag
    26941 aagcattatc tgaactagaa cggaaagaaa ataagaaata aaaagtgtat tttttctgaa
    27001 aatgtgctag aatttttatg taagccatag aacaagttga tcgaggtgga aattttggca
    27061 agcaaaagag ttgctggtac aattatgttg catttggagg atggtactaa aaaatttttg
    27121 gtccattcaa ttgatgacaa gttggaattt gcgttagcgg aactttctga aggaaagact
    27181 ggtttagcga atatacttaa tttcttaaaa gaaatagttc atatcgatgt tagcaagatt
    27241 agtttgatgg aattgacaaa tacacatatt aaagaggaaa atgttccgtt gtttgtcttt
    27301 gaaactgaac aaaagaatca aagagaagaa ttaggcgaag gatatatttg ggaagaacca
    27361 ggtcaaatgc gctctgttct tggaacttac gaagtagagg gagtgccttt cttctaaaat
    27421 acagattaag cgaacaaaga agtttgaatt tttttcaaac ttcttttttt attgtttggt
    27481 ccaaaaaata caatgaaatg ttctgctaaa gatagttttt tagtgcattt tattttcaaa
    27541 atatgttgaa aacggttgta taacgaataa taataattga tcgaaaaatc atttcacaaa
    27601 gtaatgctta atttatagtt gaccaaggaa gaaatacatg ataaatttaa cagcgttgat
    27661 tctgaagtgg atgttatatc acatttctaa aaaatttaaa aaatagttat tgacatgtta
    27721 ctaacgacat gataagataa ctgagtcgtt aaaaaagaac cacttgcgaa gcttgattct
    27781 atcgaa
//
LOCUS       DAXWLG010000021        27240 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXWLG010000021 DAXWLG010000000
VERSION     DAXWLG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.021"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(37..405)
                     /gene="rplL"
                     /locus_tag="ACGE4T_000680"
     CDS             complement(37..405)
                     /gene="rplL"
                     /locus_tag="ACGE4T_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFF8385986.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(458..961)
                     /gene="rplJ"
                     /locus_tag="ACGE4T_000681"
     CDS             complement(458..961)
                     /gene="rplJ"
                     /locus_tag="ACGE4T_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFF8385987.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(1165..1292)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1371..2060)
                     /gene="rplA"
                     /locus_tag="ACGE4T_000682"
     CDS             complement(1371..2060)
                     /gene="rplA"
                     /locus_tag="ACGE4T_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFF8385988.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2164..2586)
                     /gene="rplK"
                     /locus_tag="ACGE4T_000683"
     CDS             complement(2164..2586)
                     /gene="rplK"
                     /locus_tag="ACGE4T_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFF8385989.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2929..3597
                     /gene="sdaAB"
                     /locus_tag="ACGE4T_000684"
     CDS             2929..3597
                     /gene="sdaAB"
                     /locus_tag="ACGE4T_000684"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFF8385990.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3610..4482
                     /gene="sdaAA"
                     /locus_tag="ACGE4T_000685"
     CDS             3610..4482
                     /gene="sdaAA"
                     /locus_tag="ACGE4T_000685"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFF8385991.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4573..5673)
                     /locus_tag="ACGE4T_000686"
                     /pseudo
     CDS             complement(4573..5673)
                     /locus_tag="ACGE4T_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(5765..6652)
                     /locus_tag="ACGE4T_000687"
     CDS             complement(5765..6652)
                     /locus_tag="ACGE4T_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8385992.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(6729..7274)
                     /gene="nusG"
                     /locus_tag="ACGE4T_000688"
     CDS             complement(6729..7274)
                     /gene="nusG"
                     /locus_tag="ACGE4T_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFF8385993.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7375..7545)
                     /gene="secE"
                     /locus_tag="ACGE4T_000689"
     CDS             complement(7375..7545)
                     /gene="secE"
                     /locus_tag="ACGE4T_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFF8385994.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7569..7721)
                     /gene="rpmG"
                     /locus_tag="ACGE4T_000690"
     CDS             complement(7569..7721)
                     /gene="rpmG"
                     /locus_tag="ACGE4T_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8385995.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7883..8527
                     /gene="cbpA"
                     /locus_tag="ACGE4T_000691"
     CDS             7883..8527
                     /gene="cbpA"
                     /locus_tag="ACGE4T_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFF8385996.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8583..9086)
                     /locus_tag="ACGE4T_000692"
     CDS             complement(8583..9086)
                     /locus_tag="ACGE4T_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8385997.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9099..9145)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9683..10648
                     /locus_tag="ACGE4T_000693"
     CDS             9683..10648
                     /locus_tag="ACGE4T_000693"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8385998.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10747..11919)
                     /locus_tag="ACGE4T_000694"
     CDS             complement(10747..11919)
                     /locus_tag="ACGE4T_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8385999.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11906..13051)
                     /locus_tag="ACGE4T_000695"
     CDS             complement(11906..13051)
                     /locus_tag="ACGE4T_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386000.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(13065..13997)
                     /locus_tag="ACGE4T_000696"
     CDS             complement(13065..13997)
                     /locus_tag="ACGE4T_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386001.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            14114..15193
                     /locus_tag="ACGE4T_000697"
     CDS             14114..15193
                     /locus_tag="ACGE4T_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8386002.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            15190..15819
                     /locus_tag="ACGE4T_000698"
     CDS             15190..15819
                     /locus_tag="ACGE4T_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8386003.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(15929..16156)
                     /locus_tag="ACGE4T_000699"
     CDS             complement(15929..16156)
                     /locus_tag="ACGE4T_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8386004.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(16259..18847)
                     /locus_tag="ACGE4T_000700"
     CDS             complement(16259..18847)
                     /locus_tag="ACGE4T_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFF8386005.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(18867..19526)
                     /locus_tag="ACGE4T_000701"
     CDS             complement(18867..19526)
                     /locus_tag="ACGE4T_000701"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8386006.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(19575..20078)
                     /gene="trmL"
                     /locus_tag="ACGE4T_000702"
     CDS             complement(19575..20078)
                     /gene="trmL"
                     /locus_tag="ACGE4T_000702"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFF8386007.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(20299..21165)
                     /locus_tag="ACGE4T_000703"
     CDS             complement(20299..21165)
                     /locus_tag="ACGE4T_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8386008.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            21274..21915
                     /locus_tag="ACGE4T_000704"
     CDS             21274..21915
                     /locus_tag="ACGE4T_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFF8386009.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(21977..23338)
                     /gene="mgtE"
                     /locus_tag="ACGE4T_000705"
     CDS             complement(21977..23338)
                     /gene="mgtE"
                     /locus_tag="ACGE4T_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFF8386010.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(23356..24255)
                     /locus_tag="ACGE4T_000706"
     CDS             complement(23356..24255)
                     /locus_tag="ACGE4T_000706"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8386011.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKSYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(24257..25054)
                     /locus_tag="ACGE4T_000707"
     CDS             complement(24257..25054)
                     /locus_tag="ACGE4T_000707"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFF8386012.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(25032..25736)
                     /locus_tag="ACGE4T_000708"
     CDS             complement(25032..25736)
                     /locus_tag="ACGE4T_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFF8386013.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            25867..26442
                     /locus_tag="ACGE4T_000709"
     CDS             25867..26442
                     /locus_tag="ACGE4T_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFF8386014.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            26560..27237
                     /locus_tag="ACGE4T_000710"
     CDS             26560..27237
                     /locus_tag="ACGE4T_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFF8386015.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         7934 a         5749 c         4908 g         8649 t
ORIGIN      
        1 ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc
       61 aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac
      121 tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc
      181 acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa
      241 ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc
      301 agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt
      361 tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt
      421 ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt
      481 tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata
      541 gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca
      601 atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag
      661 caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg
      721 taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa
      781 gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac
      841 gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg
      901 taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca
      961 tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc
     1021 acttttatag ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt
     1081 gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca
     1141 tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat
     1201 agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca
     1261 cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt
     1321 tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa
     1381 gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat
     1441 tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt
     1501 tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct
     1561 ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc
     1621 attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct
     1681 agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat
     1741 ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca
     1801 gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg
     1861 tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa
     1921 cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct
     1981 actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt
     2041 tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc
     2101 ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat
     2161 agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc
     2221 ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc
     2281 gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc
     2341 tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc
     2401 ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt
     2461 gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg
     2521 tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt
     2581 tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc
     2641 tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact
     2701 ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt
     2761 gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct
     2821 tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt
     2881 ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta
     2941 aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac
     3001 actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat
     3061 tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat
     3121 attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct
     3181 ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc
     3241 gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact
     3301 ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct
     3361 ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct
     3421 aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg
     3481 gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa
     3541 acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga
     3601 gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt
     3661 gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc
     3721 atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa
     3781 ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta
     3841 aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct
     3901 gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg
     3961 ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag
     4021 caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct
     4081 atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca
     4141 gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt
     4201 acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg
     4261 tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta
     4321 gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga
     4381 cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact
     4441 gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg
     4501 agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct
     4561 cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg tttttgttcc
     4621 tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc
     4681 ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc
     4741 accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac
     4801 tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc
     4861 tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat
     4921 agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat
     4981 ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc
     5041 tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt
     5101 aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag
     5161 cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat
     5221 gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg
     5281 cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga
     5341 tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg
     5401 ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg
     5461 caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga
     5521 agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc
     5581 tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat
     5641 tggatgttct tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat
     5701 tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt
     5761 atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa
     5821 gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt
     5881 gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt
     5941 ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga
     6001 tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat
     6061 ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta
     6121 atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac
     6181 taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca
     6241 gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt
     6301 caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta
     6361 tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca
     6421 aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg
     6481 ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct ttatcagaaa
     6541 cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga
     6601 ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct
     6661 tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag
     6721 tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt
     6781 ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt
     6841 ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct
     6901 agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga
     6961 agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt
     7021 gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct
     7081 gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct
     7141 tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg
     7201 atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt
     7261 tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc
     7321 cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt
     7381 taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa
     7441 aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca
     7501 agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt
     7561 gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta
     7621 ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac
     7681 ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct
     7741 gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa
     7801 ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa
     7861 tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct
     7921 gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg
     7981 ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa
     8041 aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct
     8101 tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac
     8161 gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt
     8221 gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta
     8281 tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat
     8341 ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat
     8401 tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt
     8461 caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga
     8521 agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat
     8581 aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa
     8641 acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg
     8701 ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg
     8761 ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct
     8821 aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag
     8881 gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact
     8941 aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact
     9001 aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat tacggataac
     9061 cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc
     9121 gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt
     9181 tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg
     9241 ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa
     9301 gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg
     9361 atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa
     9421 gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg cgcttgctag
     9481 gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc tgctataata
     9541 accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc attcaaaaaa
     9601 tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa tatcttgttt
     9661 tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca atccatcggc
     9721 aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa aaaataagca
     9781 tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga ttgctgaaac
     9841 tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa tcgatacttt
     9901 cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg atactttcta
     9961 tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa atgattttgc
    10021 tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt tagcagctca
    10081 atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat
    10141 agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga caaatcattc
    10201 ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt tattctctat
    10261 tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag gaggaaaaca
    10321 attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt ttggcggagg
    10381 aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg tcgtggtcga
    10441 acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga aaaaacaatt
    10501 gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt caacaacacc
    10561 gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata tcacaataac
    10621 cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc tacatctgga
    10681 acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc tttttttact
    10741 atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt tacccaaaat
    10801 aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa
    10861 agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat tattttgaac
    10921 aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca agtattctgc
    10981 tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt tgatcccacc
    11041 gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa
    11101 taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg aggtgtggat
    11161 cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt aactaatcaa
    11221 actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt
    11281 actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa aaatggatag
    11341 aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt tcatccgctg
    11401 actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt gtgtctgttc
    11461 catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt
    11521 gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct gtatcttgat
    11581 cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat gtaagataat
    11641 gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc catccaatgt
    11701 aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag gcgactcttc
    11761 ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat caatattttg
    11821 cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat tcgatttatt
    11881 tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct ctttccatcc
    11941 aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga ttggtataaa
    12001 aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc aagttgacga
    12061 gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact tgttccgacc
    12121 ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt
    12181 gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa atcgcacaca
    12241 acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag tctacttgat
    12301 agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc gtaaagcttg
    12361 ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac
    12421 catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt
    12481 agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac
    12541 ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat
    12601 caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat
    12661 ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag
    12721 atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt
    12781 ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta
    12841 agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat
    12901 taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc
    12961 ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt
    13021 ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc
    13081 ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg
    13141 taactgatct tttcttcttg taagattgta agaatcggca acaatactgt atttagttca
    13201 gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc
    13261 tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc
    13321 gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg tccttgatcc
    13381 atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt tgtatagaca
    13441 atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt ccgactttgg
    13501 gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca
    13561 catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg aaacgaaaca
    13621 tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct tttctctttt
    13681 tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag ttcgtcaaag
    13741 accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt ttcaggagtc
    13801 attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga aagcatacag
    13861 ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac ttctccttct
    13921 tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt gactaattta
    13981 tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc tgactataat
    14041 aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat ttgtcacaat
    14101 gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc tactggttat
    14161 ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct atttattgat
    14221 cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt ggattttcca
    14281 agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc tgccagcgac
    14341 gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt taggtttttt
    14401 gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg taattttgct
    14461 gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta
    14521 cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac aaaataaagc
    14581 attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac ggaaccgaat
    14641 tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt tacaaattgg
    14701 tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa atttaagtgc
    14761 caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc atgagacatc
    14821 gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt ctgttacgat
    14881 tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct taatggtcat
    14941 caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt
    15001 tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca gcccacaaaa
    15061 aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg
    15121 acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt taccaaggga
    15181 gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac gaaatcgtta
    15241 caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca ttgtgccaag
    15301 gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    15361 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    15421 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    15481 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    15541 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    15601 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    15661 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    15721 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    15781 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    15841 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    15901 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    15961 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    16021 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    16081 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    16141 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    16201 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    16261 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    16321 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    16381 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    16441 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    16501 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    16561 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    16621 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    16681 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    16741 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    16801 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    16861 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    16921 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    16981 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    17041 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    17101 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    17161 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    17221 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    17281 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    17341 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    17401 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    17461 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    17521 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    17581 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    17641 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    17701 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    17761 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    17821 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    17881 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    17941 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    18001 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    18061 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    18121 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    18181 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    18241 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    18301 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    18361 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    18421 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    18481 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    18541 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    18601 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    18661 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    18721 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    18781 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    18841 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    18901 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    18961 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    19021 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    19081 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    19141 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    19201 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    19261 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    19321 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    19381 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    19441 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    19501 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    19561 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    19621 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    19681 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    19741 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    19801 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    19861 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    19921 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    19981 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    20041 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    20101 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    20161 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    20221 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    20281 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    20341 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    20401 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    20461 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    20521 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    20581 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    20641 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    20701 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    20761 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    20821 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    20881 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    20941 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    21001 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    21061 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    21121 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    21181 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    21241 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    21301 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    21361 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    21421 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    21481 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    21541 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    21601 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    21661 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    21721 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    21781 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    21841 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    21901 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    21961 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    22021 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    22081 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    22141 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    22201 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    22261 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    22321 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    22381 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    22441 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    22501 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    22561 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    22621 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    22681 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    22741 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    22801 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    22861 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    22921 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    22981 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    23041 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    23101 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    23161 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    23221 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    23281 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    23341 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    23401 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    23461 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    23521 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    23581 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    23641 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    23701 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    23761 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    23821 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    23881 ttggttttcg taaccttgtt ttttataata agactttaca cgattagcca ttgtcccatt
    23941 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    24001 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    24061 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    24121 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    24181 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    24241 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    24301 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    24361 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    24421 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    24481 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    24541 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    24601 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    24661 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    24721 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    24781 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    24841 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    24901 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    24961 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    25021 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    25081 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    25141 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    25201 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    25261 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    25321 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    25381 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    25441 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    25501 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    25561 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    25621 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    25681 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    25741 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    25801 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    25861 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    25921 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    25981 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    26041 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    26101 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    26161 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    26221 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    26281 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    26341 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    26401 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    26461 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    26521 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    26581 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    26641 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    26701 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    26761 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    26821 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    26881 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    26941 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    27001 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    27061 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    27121 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    27181 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAXWLG010000022        27157 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXWLG010000022 DAXWLG010000000
VERSION     DAXWLG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.022"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(172..1590)
                     /locus_tag="ACGE4T_000711"
     CDS             complement(172..1590)
                     /locus_tag="ACGE4T_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8386016.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIDGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(1830..3398)
                     /locus_tag="ACGE4T_000712"
     CDS             complement(1830..3398)
                     /locus_tag="ACGE4T_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFF8386017.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(3402..3812)
                     /locus_tag="ACGE4T_000713"
     CDS             complement(3402..3812)
                     /locus_tag="ACGE4T_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFF8386018.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(3809..4732)
                     /locus_tag="ACGE4T_000714"
     CDS             complement(3809..4732)
                     /locus_tag="ACGE4T_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFF8386019.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(4729..5607)
                     /locus_tag="ACGE4T_000715"
     CDS             complement(4729..5607)
                     /locus_tag="ACGE4T_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFF8386020.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(5594..6931)
                     /locus_tag="ACGE4T_000716"
     CDS             complement(5594..6931)
                     /locus_tag="ACGE4T_000716"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8386021.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(6934..8037)
                     /locus_tag="ACGE4T_000717"
     CDS             complement(6934..8037)
                     /locus_tag="ACGE4T_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8386022.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(8111..9619)
                     /locus_tag="ACGE4T_000718"
     CDS             complement(8111..9619)
                     /locus_tag="ACGE4T_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8386023.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(9585..10277)
                     /locus_tag="ACGE4T_000719"
     CDS             complement(9585..10277)
                     /locus_tag="ACGE4T_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8386024.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(10299..11273)
                     /locus_tag="ACGE4T_000720"
     CDS             complement(10299..11273)
                     /locus_tag="ACGE4T_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386025.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            11573..12445
                     /locus_tag="ACGE4T_000721"
     CDS             11573..12445
                     /locus_tag="ACGE4T_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386026.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(12903..15101)
                     /locus_tag="ACGE4T_000722"
     CDS             complement(12903..15101)
                     /locus_tag="ACGE4T_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386027.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            15333..16325
                     /locus_tag="ACGE4T_000723"
     CDS             15333..16325
                     /locus_tag="ACGE4T_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8386028.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            16385..17806
                     /locus_tag="ACGE4T_000724"
     CDS             16385..17806
                     /locus_tag="ACGE4T_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFF8386029.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            17799..19298
                     /locus_tag="ACGE4T_000725"
     CDS             17799..19298
                     /locus_tag="ACGE4T_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFF8386030.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            19337..20731
                     /gene="uxaC"
                     /locus_tag="ACGE4T_000726"
     CDS             19337..20731
                     /gene="uxaC"
                     /locus_tag="ACGE4T_000726"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFF8386031.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            20890..21573
                     /locus_tag="ACGE4T_000727"
     CDS             20890..21573
                     /locus_tag="ACGE4T_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFF8386032.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            21575..22411
                     /locus_tag="ACGE4T_000728"
     CDS             21575..22411
                     /locus_tag="ACGE4T_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8386033.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            22422..24176
                     /locus_tag="ACGE4T_000729"
     CDS             22422..24176
                     /locus_tag="ACGE4T_000729"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386034.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(24452..24793)
                     /locus_tag="ACGE4T_000730"
     CDS             complement(24452..24793)
                     /locus_tag="ACGE4T_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8386035.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(24861..27032)
                     /locus_tag="ACGE4T_000731"
     CDS             complement(24861..27032)
                     /locus_tag="ACGE4T_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8386036.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
BASE COUNT         8110 a         5574 c         4816 g         8657 t
ORIGIN      
        1 aaaaaagggc tccgactaaa taaatgcctt ttggaataaa tacaggtaag tgttctttcg
       61 caccttgatc gattgctttt tgcagtatta ttgtatcatc ttgttgagtg tcgtttgttt
      121 taaagttcaa agcattgata atcccttttt ttgtccgcaa tacgtttctc cctatctttc
      181 attgattttt ttcaattctt caccaataaa tacagttttt gaacgattca gtgggtaacc
      241 tactagcaaa atgattgcgg atacaaataa gcagaatgct ggtaacaagt ttgccatcgt
      301 ataaatcctt tcttgtacta aaactgattg gatggctcca cctgttgtag acgattggta
      361 accaatgagt gccagcataa atccgcctac cgctcctgca catgcttgtc ctacttttct
      421 cgcaaaagaa tttactccat atactgttcc atcttctctt aagcctgtct tatattgatg
      481 ataatcaatc acgtcagtaa tgaatgccca gaccatcaaa ttgaataaac cagatcctaa
      541 agtagcgata aataaaagaa tcagatatac ccatgcattt gttatatgaa ggaaatacat
      601 cagcaagtac atacatgaag aagcaagtaa cgcaaatacg cttgcttcct ttttaccaaa
      661 taattttgtc agctttgtag caaatggtgc taaaactact actgttccgt aagtaaataa
      721 aagagcaaca gacattgctt gtttattatg gaagtaatca ttgaataaat aagtcatatt
      781 cgtcccagct aaaatctgat tgatcacaat gaaaatgtca acaatgacta aaataatcag
      841 tgctcgatta gctaacaaac cttttaccaa ctgagaagca gatacttttt cgtttttgac
      901 aactcttact ctttcagtag tcaatttaca agttaacata tatgcgacaa atgctattgc
      961 agcacatccg attgaaatcc aaaagaaatg attccctgaa acgacttgtt gtccactctt
     1021 gttatgggta tacattaaaa tcggaatgaa gaatcctgta gttgatccgt caatcgcaga
     1081 accaatgctt cggaacgtgg acaacgaggt gcggtcatta ggatttgagc taattgctgc
     1141 tgccattgct ccatacggga tattgattgt tgagagaaaa atcccataaa caatgtatga
     1201 aacaaaaata taagcaatct tcaccgtcat tggaagatgt tggacgaatg gcaaaaacaa
     1261 aatgacagtc actaagcaaa atggatactt catttttctg atccaaggaa taaatcggcc
     1321 agtttctgtt aactctgaga catcacataa tcttccaacc atcacatcag caaacgcatc
     1381 caaaattctc gaaatcaaga ataacgtacc tacgattgcc cctgaaacgc caagaacatt
     1441 cgtataataa atcatcaaga aactattgac taaacctaaa ataaaacagt tacctagatc
     1501 accgaacatg tagcctatct tatcggaaat cccaaattcc tttttttctt tttttattgt
     1561 atttgctgta acagcatttt gtgaaagcat ttgaattcct cctacctctt ctagtaaacg
     1621 ttttcaaaat tataactctt tttttcttgt ttttatattc acaatgttgg ctttttattg
     1681 cattatcttg acagataaaa aacaaaaaat aatctaaaaa cattgatgca taagattgat
     1741 aacttttttt acataacaat atatacaaac aaaaaaattc actaactagt gaaaataagt
     1801 tagtgaacct catttattaa agaattaaat tacagatgat cttttccatc atacttgaaa
     1861 aactcaaaat cagcataact tcctcttttt ttatttagat catgcactgc tatcccgata
     1921 aaatttccag taaaccctcc tgagagtata cacatgtttt tttgcggacc tactttcttc
     1981 cattcttcag ttgatacgtc tttcaaatga aactgtgctg tctctttctt tacagtgact
     2041 ttcaaaaaag tcttcttttc tgtgagtgga atcgtccaat cttctatttg tgctactccg
     2101 ttttctgatt ctatcatacg taggcatttt cccttgtcct cattccaagt aacataacaa
     2161 taaatataat tggattcatt taagtacagt accagtccag ccatctgatt aaaattatta
     2221 ggcatgtatt caaatgctgt ctccgcatca aaagtaaaat ccgtttgccg aatagctaac
     2281 aaatgatgtt cgaaggtaga ttgaagagat tcaccggaaa tcagacgtaa aaaacctggt
     2341 ctacattttg tatcacacca atcagcattt ggcaaaatgc gtctggcatt ccagttcttc
     2401 tttagctctc cttcaaatgt atccataaac tgatgatctt tttcttgttg tacctcttca
     2461 tttgtctcaa tcattatttc ttccatcgga gttgtctggc tatctttaag tcttaaccaa
     2521 tcgtcggacg tccaatacac ttcttgaata gcggtttctc ttcccaaaat agccgcaccg
     2581 tttaatggtc tggtacaaag atatgtcatg taccatcttc ctgacggggt ttgtactaag
     2641 ctcccatgtc ctgtacattg caatgtagaa gcaggtttgt cactagctgt cagcatagga
     2701 aaaccaggat caagctcgta aggaccagta atattctttg ctcggcaaac tgtaactgaa
     2761 tgtcctcttc ctgtaccgcc ttctgccgtc atcaaataat aatacccgtt acgaaaatac
     2821 aaatgtggtg cctccgtttt cgctaattct gtacctgcaa atattttata gatgggacca
     2881 tcaagcttct tcttctctgc atcatattct tgtaatacga taccgctgga tttattcggt
     2941 gtgtccagac gataatccca tagttcattg acaagccatt tttttcccgt ttcaagatca
     3001 tggaacaaag aaggatcaaa gccgttacta tttaaataaa ttggttcgga ccaaggacct
     3061 gtgatctcag atgcagtgat caaataattg tggacatctt taaacggacg gcctgcactt
     3121 ttgacatctg tgtaaacaag ataaaacaat ccatcagcat aactgatctg tggcgcccat
     3181 atacttccat ttgtaggatt tccttgcaaa gatacttgag tggtcaaaat atccgttgta
     3241 tgtgaccagt tgactaaatt tttcgactca tatactcgga ttcctggaag ccattcaaaa
     3301 gaggagacaa taatgtaata ccgctctttc actcttaata tattaggatc tggattgaat
     3361 cctcttaaaa taggattttt cactttgata gtctgcatga ttcaggctcc ttttctcatt
     3421 cttactcgat ataaactgaa tgccccagct gcgaaataaa aaaatgcaaa aggaaacgca
     3481 ataaactgag tgccaatcaa caatatgaat cctgttataa aatacaaacc agtggcagtt
     3541 gttaagtatt ttcctttagc agtcaaaaac aatgctactg ctaaaaaagc aagtgaaata
     3601 aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt attttctaaa
     3661 gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc tgacgcagac
     3721 atattactta aagtaactgc taacccgcca aagaaaatca aagatagact gccaccgacc
     3781 attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt tctaaatgta
     3841 tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag aatttgacag
     3901 tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca tgcccaccta
     3961 tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct aaatagacgt
     4021 ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct gttttttgat
     4081 ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag ctatggatat
     4141 gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt ccttcaatcc
     4201 agcaatattc ataggtctga cgacaatact caggattggc agaagccggt gtacaaaaag
     4261 gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc tgataaccag
     4321 atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca acagcctgtc
     4381 ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat acgacattcg
     4441 atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg tcaagatact
     4501 cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca agtttaaaat
     4561 tggataatct gctttctata tattcatcat atcttccttc ttttactatc aagacctttg
     4621 gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt tcaaaatccg
     4681 tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc ataaatgctc
     4741 ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac cgcctgttaa
     4801 agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt ccagcccttt
     4861 tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa ctaagctaag
     4921 acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg tcaatagatt
     4981 tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa gtatttcgat
     5041 gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg ctgtcaccca
     5101 ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag tatctgtgtc
     5161 ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca ttccccataa
     5221 cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac ctccgcgttc
     5281 ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca gtacttctgc
     5341 aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc cgactgaaaa
     5401 atctccgccg tgagaatgca ggtaaaggac cggcggaaac ggtccttctt tttctggata
     5461 agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt attcaagcat
     5521 aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc tgctagatgg
     5581 agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc agttcatcca
     5641 tattttctga ttgatttgat tggaatttct caatactgat ttctttagta ttttctaata
     5701 gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa
     5761 agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata gcaggaacac
     5821 cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt tcttgtatga
     5881 attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt gccgtgatat
     5941 tcgaaaaatg gatccttctg aatgctggtg tccgttcatc tatcggataa gccttttttt
     6001 cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc agtataaatg
     6061 gacacatcac attatccatg actatattgg atactcgaat atcttcaacg atcccgcctc
     6121 gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata caatttgaaa
     6181 tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg ccatgtccat
     6241 ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg tacgtatctt
     6301 cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa ttactaatac
     6361 gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc gctgggttca
     6421 agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc gtccaacttg
     6481 gtgactggat caatttaata tctttcacag taattcgatg acagttatgg aaactcatca
     6541 atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc caccattttt
     6601 ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca ttttgagcat
     6661 agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga acaacaggat
     6721 aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga agttcgacat
     6781 tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact acggtcccgc
     6841 ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc aattcatcaa
     6901 ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt caatcaatgc
     6961 ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac tgatgtagta
     7021 agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac agtttttatt
     7081 caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc cttgatgtga
     7141 tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta ttcctttagc
     7201 cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt tccgttttgt
     7261 ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa gtaatgcagc
     7321 cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat gaagtaattc
     7381 ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa aatgatggga
     7441 tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg catgaatcag
     7501 caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc tcaaaacatc
     7561 ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag gagaacccat
     7621 atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa aagctccttc
     7681 ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt ttttatattt
     7741 ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta catggtcgat
     7801 attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga aatagtcgat
     7861 attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa gtaattggaa
     7921 ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact tttcttcata
     7981 taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt tttccattat
     8041 ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt atttgtccca
     8101 atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt gtgattcgat
     8161 cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg tcaagctgtc
     8221 ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat cctacttcat
     8281 tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct tcattttctt
     8341 tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag aacgtcactt
     8401 cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc atatcgataa
     8461 attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg ccagtcatca
     8521 atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc ttcaattgtg
     8581 cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca tttccttctg
     8641 accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc actaattcca
     8701 tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc attccagtgt
     8761 agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa
     8821 agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa gctttcatat
     8881 actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc cagccatttg
     8941 gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca ctaggcgcac
     9001 gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt tctgtaaatt
     9061 gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct agattatcca
     9121 accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc ggtacgccgt
     9181 acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga cttaaatttt
     9241 tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac gcattgcgga
     9301 cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt tcatcagaag
     9361 ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg aatgtcagtt
     9421 ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt gaagcatgat
     9481 gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa gaactgttac
     9541 ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga cctaatttta
     9601 cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt tttgttttct
     9661 tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga atttgctgtc
     9721 cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc tggcattgtt
     9781 tggatgatca tcagataaat ccctaccata cttaatatca acactaatga gtaatgaaga
     9841 tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt ccaatcataa
     9901 tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc agcaatatac
     9961 aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa aacaatcccg
    10021 atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt aaaatatgtg
    10081 aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct aatcactgca
    10141 accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat gactaaaccg
    10201 ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc gtttatttta
    10261 tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc gtcttctcct
    10321 agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac ccctttaaac
    10381 aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt ataaatatag
    10441 gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta catatgctcg
    10501 aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat tactggacga
    10561 attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc catttctgca
    10621 gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat cgtagaccag
    10681 cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc aggggatgtt
    10741 agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac ccctccgtca
    10801 acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa gtgagggatg
    10861 taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt taatagtaaa
    10921 gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa gatcaacgta
    10981 tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg
    11041 acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc aataacgagt
    11101 ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag aatcatcaca
    11161 tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct gagcttttct
    11221 tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc cataagcccc
    11281 tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta tctgaattac
    11341 gtatataatc atttttaccg taagtaaaaa cgcttacata gcaatgattt ttgtgctata
    11401 caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct tctatttttt
    11461 tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg atgataaatt
    11521 gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct atatgaaaaa
    11581 gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc taattttgct
    11641 agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg cttctacaca
    11701 agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag acgcgtatcg
    11761 ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg gtgttacagg
    11821 tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa
    11881 gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg
    11941 ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt cttactgggc
    12001 catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa actttttcca
    12061 agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag attttggtgt
    12121 atttttcaaa attatgctgc cattagcgtt accgtctatt gctactatct cgctttttta
    12181 tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg attcatctaa
    12241 atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga tgcaagcaga
    12301 aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg ttatcgttat
    12361 tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg tcaaaggagc
    12421 actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag ttgtgtcaga
    12481 agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa atttttgtga
    12541 aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag aggttgtgaa
    12601 agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac gagtaaccca
    12661 caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa attatgcctt
    12721 attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg tgacaagact
    12781 tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata aatacaaata
    12841 ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa cagcctcttg
    12901 attcacaaat atttttttcg atattctccc ggcgtaatcg attccttctt tttaaagaaa
    12961 cgaataaaat tttgcggatt tcgatattgc aatctttctg caatctcttt tacggagagg
    13021 ttagtctctt tcaaccattt ttttgctacc tctaaacgat gattttgaac aaaatcacca
    13081 aatttttctc ctgtttcctt tttaaataca ttacttaaat agttagggtt ataatgcaac
    13141 ctatcagcga tcacatctaa agacagatca cgatcatatt ctgtgtagac gatatgcata
    13201 atattctccg acaaattttt aaattcatga tcagtgattg cttgagagca ttctgtgatt
    13261 ggcagaacta attgttctaa aacaagtttt tctagttctt tgggatgata attgttcagt
    13321 gcttttacat aaatttgttt catgttatca aagttttttg aatctactcc caataactgt
    13381 cctaattgaa cgagttcatt gatcaagcgg accgaaacta cttcccgact caaaggattt
    13441 ttattttgtg caaacaatgc atcaatcaat tcatgtacta aattcttcac ttcctgattt
    13501 ccagaacgaa tggcttcaaa caatcgattt tgttcttcta tcggatatct aataagtgtt
    13561 ttttcatgag cagcaggaac gatttcctga taaaaaataa ttgaacttgg accagtattt
    13621 acacgataat acagggcttc ttttgcccga tcaaccgatt gcttgctttc attcagcgtt
    13681 tcgaaccggt tactgatgcc tatactgata gttaaattca agtattcttt tacacgtttt
    13741 tgaatgagtc ggcaatactc catgactttt ttgcttgctt ctgcatcatc tttatccatt
    13801 cgataaatag tggcttgcat ttcttcattc aaaacgatcg gcagcattcg attttccttt
    13861 ggcacaattt ccgtgatcat gttgttgatc gctaaaagta gcaaatcacg ctccccaccg
    13921 tgactatctt ctaacagatc gatttgaacc aaagctgtat aatagcagtc attttgagaa
    13981 ctataaccaa attgctgcaa ccgctgatct agttctcgcc tttctactcg atttctaaat
    14041 aaactaagta caaatagcgt ttccagctgt ggcttttggg taactaagtg tgcacttaac
    14101 gcttcttttt ctcctatgat ccgactgact gattgagcga ccaaatctag ttctttccca
    14161 ctgaatcctt ttgatttaag attcagacgt tcctgtattt gagaaatcgg acgggcaaat
    14221 ctgtccgaaa ataagtagga aagaagacca atgagtccta ccagtgttaa agacactgca
    14281 actagcccga ttctcagatt gcggatcatt gcgccaattg cactagagtc aacttctaat
    14341 ctgtaaatcc aattattata atctgatttc agtactatat acgttttatc tttttctctt
    14401 atcactttca gctgattgta atctagatcc acgtccgtta atacagttgg ttgctcttta
    14461 ttttttactg tctgagtagt aaaaaggttg ccaacttcgt tttcaataga aagtatgtct
    14521 tctccacttt caccaacaac ttgttctatt gtatgtttct ctatcgtagc aattcccaat
    14581 gcatattttt ctttttcaaa cattggcaaa gaaatcacca tttcgattcc tttgtttttc
    14641 ttttgccaaa ataaattatt tttgttattt atgtacttct gttgatactt ttcaatttcc
    14701 tcttcggtca gttgactcaa cgatccattg attactcccc atttaccagc caaactgact
    14761 aaatcataat tattatcatc cataacaatc gtttctataa aatttagttc tttcctgatt
    14821 tcagaataag tggagtaatc tgtatatgtc agcggatgct tgaatatgtc cgagaaattt
    14881 ttggtcgaac catacgtaga aaacgaatat tcaatcgttc taattttcca ttcaacgtta
    14941 gttttaactt gcctgagcaa attctttttg tctgttaaat aactcgattc gatcgattca
    15001 taggtattct ggtaaatgaa taaactaaat cctatgacaa gtacggctcc taataaaaac
    15061 atcggtaaaa atattttata aaatactgta cttttcttca ttttcttcct ctcctccttt
    15121 taaaataaaa ggctttcatt tttctgtaaa attgatactc atctaattaa atgagcattc
    15181 cccgctttca agaatagcat aattaaaaat aaaaatccaa gtaattttta acgtgtgcaa
    15241 tcttttcata tcaaagatct attgacttat cgtagttatc gtaatatgat gaatgcgtta
    15301 acaaaaagtg aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat gtcgctaaag
    15361 cagctggcgt ttctcatacg acagtttctc gtgctttgaa taacagcagt ttaataaaaa
    15421 atgaaacaaa acaaaaaata cgacaactag ctaaggaaat gaattattcg ccaaactata
    15481 gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg actatttttc tcaagtttgc
    15541 ataaaggaac ttcatcaaat ttcctagcag attccattgc aggcatccat tcagtattag
    15601 acacaaatta cagtttatct gttgaaagta tcgatgttgt tgatttaaca gaaatcaacc
    15661 tgcaacgcta cgatggactg atcgttatga gtcaaagtga tgatgatcag cctttcatcg
    15721 actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc aatgatccag
    15781 cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta gattacggta
    15841 ttggatgcgg acatacgaag atcgcttata ttggcggaaa gaaaaatttt cgttcgacaa
    15901 aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct gtcaatccag
    15961 cctatttttt aacaggagat tacagcttag aaagcggcct ccaaaacatg gaatacctat
    16021 tatcactttc tgacctgcct actctggttt tttgcggcaa cgacgacata gcgattggtg
    16081 cattgagagc tgtacattta gctggcctcc gtgttcccca gaacatttct ctgatcgggt
    16141 tcgatgattc tccagtcgtc tcctatctca atccgccgct taccaccgta cgtaagcctt
    16201 tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa ggcaggacga
    16261 tcgaacaatc taaaattgag ctctccacct tgttgatcct tcgtgagtcg atcaattact
    16321 tgtaaactac aagtaatttt atataaaaat gttaacgtgt gcataacaca gaaaggaagt
    16381 ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac gaagcacctg taaaagttct
    16441 tcagtttggt gaaggcaatt ttatgcgtgg atttattgat tggcagattc agcagctgaa
    16501 taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa cctttagaac aaggtcttgg
    16561 aaatatgatg aaccagcaag ataatttata tacggtcatt ttacaaggac tcagcaatgg
    16621 cgaaatcgtt gatacttccg agattatcac cgtagtagaa caaatgatca atccttatga
    16681 tgaatgggat gcttatctat ctctagcaga aaatgacgac ttggcctata tcgtctccaa
    16741 tacaacagag gcaggaatcc aattcgtgga gactgataat ttaacagata ctcccccaca
    16801 gagcctcccg ggcaaactga cagccttttt ataccgacgt ttcaaattaa acaaagctgg
    16861 ctttaccatc attccttgcg aactgattga cagaaacggc gaacgtctaa aagaaatcat
    16921 tttacgctac gcagaaaaat gggatttaga acctgctttt gttgagtggt tagaaaaaga
    16981 aaatgttttc tgttgtagtt tagttgatcg catcgtccca ggttatcctc gcgaagatgc
    17041 aggtgttttc aatcaacggc ataactatga agatcaattg atggtcaaag cagaaccatt
    17101 catgctttgg gtgatcgaag ggcctaaaga attggagaat caactaccat tcaaacaggc
    17161 tggcttgaat gtcatcatca cagatgatat gactccttat cgtgaacgta aggtgcattt
    17221 gcttaatgga ccgcatacag cgatggtccc tcttgctttg ttggcggaat tggaaactgt
    17281 agaagaagta atgaaagatc ctttattctc cgcatatgtt gatcagttat tcaatctaga
    17341 attgattcct atgctttctt tacctaaaga tgaattagcc atttatgctg accaaatcaa
    17401 agaacgtttc ttgaatcctt ttgcccatca caaattagaa gcgatttcat taaatagtgt
    17461 ttcaaagttc agcacccgtc tgcttccagt gttcaagaag tatatcgaag aacaaaatca
    17521 ggtaccaccg ttaatcactg tttcactagc tgcgctactt cttatgtatc gaggcgatca
    17581 agtaaagcca catgatgatg aaaagacgat aagcgaattt actgatgctt ggtctgataa
    17641 tggaacagct attcctcgat tattgcaaaa cgctgcgctt tggggagaag atttgtctca
    17701 aataccgaat gtgacagata cagttcaaga aatagctgca aagatcgatc aaaaaggtgt
    17761 ccggtcagta ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc ctgcaaccaa
    17821 caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt ccaaaacaaa
    17881 cacaactgac gattgatcag caaacgatca ccactcttga agatatcccg caaggtcata
    17941 agattgcatt gggtgatttg aaagctggag acaacgtgat caaatatggc tatccaatcg
    18001 gtcatgtgac gacagacgta actgccggtc aatggctgca cactcataat gtaaaaacca
    18061 atctttctgg tgaattggat tatcgatatg aaaaagatgt ccatccaagt cattaccttt
    18121 ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg attcgtaatg
    18181 atttgttttt agtacctacc gttgggtgtg tgaatggcat tgctgaactg atcgtcaaac
    18241 agttcaagga aaaacatcca gatctgggac agtttgacca tattacgatt ttaaagcatc
    18301 cttatggctg ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt ttagcggacg
    18361 cagtaaatca tcccaatgcc ggaggagttt tagtatttgg attaggttgt gagaacaata
    18421 cagttcctga attcaaaaaa attttaggtg aatatgatga agaacgagtg aaatttctag
    18481 tggctcaaga tgtttacgat gagatcgaac aaggtgtggc tctattagaa gagcttttaa
    18541 cagcggcgga aaatgaccag cgcatatcag tcccgctctc taagttgaat gtcggtttga
    18601 aatgcggcgg ttctgatggt ctatcgggaa taactgctaa tcctttactt ggtgcatttt
    18661 ctgactactt gattgctcaa ggaggcagca cgatcttaac agaagtgcca gaaatgtttg
    18721 gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga agtttttgac tcaatcgttg
    18781 acttgatcaa tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt tacgaaaacc
    18841 catctcctgg taataaagca ggtgggatca ctacattaga agataagtct ttaggctgca
    18901 cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca atatggcgaa aaaatcagaa
    18961 aaccagggct tagtttattg caagctcccg ggaatgatct tgtagctgcc tctgctcttg
    19021 cttcatccga ttgccagttg gtcttgttta ccacaggaag agggactccg tttggctcat
    19081 atgtcccaac ggtcaaggta tctacgaata caactctttt tgaacgcaaa ggtcattgga
    19141 tggattttaa tgcaggcgtc ttattagaac aaccaatgga agtaatctta gaatcgtttg
    19201 ttcaaaaaat catcgctgta gctagtggag aagaaacgaa aaatgaacaa aatgacgttc
    19261 gagaaattgc catttttaaa aacggagtaa cactttgatt ttcataaaac agtataaaaa
    19321 gaaagaagga agatttatgt ttttatctga tgatttttta ttaaaagacg attgggcgaa
    19381 aaagctttac cattcctacg caaaaaacat gcccattatt gattatcact gtcacttaga
    19441 tccaaaagaa atttacgaaa acaacaattt cagcaatctg acagaagctt ggttaggagg
    19501 cgatcattac aaatggcgct tgatgcgagc ttgcggcgtg cctgaagaaa aaatcacagg
    19561 aaatgcttct gattttgaaa aatttttagc ttggtgtcaa accgtaccaa aaattatcgg
    19621 aaatccgctt tacagttggt cacatcttga attgaagcgt ttcttcggta ttgacttgtt
    19681 gataaatgaa gaaaatgcag agaaaatttg gacacaagca aatgaaattt tggcagctcc
    19741 tgattttcgc cgtcgggaaa tcgcaaaaaa atcgaatgta gaagtgattt gtacaacaga
    19801 tgatcccgca gacgacttgc actatcatca gttgcttgct catgaagaac cagatttgaa
    19861 ggttctacct tcattccgtc cagataaagc actcaatatt gaaaaagaag gatttaatgc
    19921 atgggtcaca ctactggaag aagcagctga ccaaacgatc accacctatc aagaactgat
    19981 agaagtctta gggcagcgga tagaatactt ccatcaacat ggatgtcgtg tttccgacca
    20041 cgctttagac atcttacgct ataaagaagc ggatgaaaca gtattagaag agatttttca
    20101 aaaagcacga gctaacaaac tattatcttc agatgaaatc gatgcttatc gtacagaaac
    20161 actgatacat ttgacccact tctatcattc tcataattgg acgatgcagc tgcacatcca
    20221 tgcttatcgt aactgtaata cacaaatgtt ggaaaaatta gggcccgata ccggctatga
    20281 tggaatgaat gatctttctt tgactattcc tctgcaaaaa ttattgaacc gtgcagaaga
    20341 aacggatcag ttgccaaaga cgatccttta ttcattgaat ccgaatgact atcctgcgat
    20401 tttagctttg atgggctgtt tccaaaaaga acagaatgga aaactgcagt taggttctgg
    20461 atggtggtac aacgatactc gagctggtat gcgcgatcaa ttgacacaat ttgctgatgg
    20521 tagtgctttg gggaattttg tcggcatgct gacagattca agaagtttcc tgtcctacac
    20581 tagacatgaa tatttccgtc gtgttctttg tgaattttta ggcgaaatgg tcatgcgagg
    20641 agaagcacct gaagatgaac aacttttagg aagtctagtt caagcgatta gttatacaaa
    20701 tgctaaaaat tactttggct tttttgccta attgattaat agtaacagta acgaatcaaa
    20761 aacaggatag tgaaataacg gaatccgttt atccacgttt tagagaagaa gacggaccaa
    20821 atttgctccg tcttcttctc ttgctgttct gcttcattgc ctataaacct ttatgaaaga
    20881 agagataaca tgcctactat ccatattgcc ggtgattcta ctgcggcaga aaaagatgaa
    20941 aacaagcgtc ccgagacagg ttggggagaa aaaatctctg cttatttttc acccgagatc
    21001 cagatcaata accaagcaaa gaatggacgc agcaccaaat catttatcca ggaaggaagg
    21061 ttagcccgtc tagcttcaca gtttcagccg aatgatttct tatttgtaca atttggacat
    21121 aacgaccaga aaatccaaga agaaagaggt acgcttccct tcggtagcta tttaaaaaat
    21181 ctgcagatat tttttgaatg cgcaaaaaaa gcgaacgtcc aaatggtctt actgacatct
    21241 gtgacgcgac gagattactt agaaaacgga acattgaatc cagatatact tggagactat
    21301 ccaaaagcga tgcgtgcatt tgctgagaaa caccatattc ctcttttaga tgtgttctca
    21361 agatcacaag aattgttcca gaccttctca aaagaagaga caaaaaaatt ctacctgcat
    21421 cttatacctg atacttgtaa aaattaccca gaaggactaa aagacaatac ccactttagt
    21481 ccagaaggcg ctgataaagt tgcccgaata attgttgaat tgataaaaga atcccgtctg
    21541 cctcttgcta attatttaca aaaaggagtt tgagatgttt ccattaaatt taggcatccg
    21601 tgcccacgat ttagatgttc gaaatcgtga agaaatcgtt caaaaagtat cacagcttca
    21661 cttgtcccat atccaatttg caccacaaaa agcatttcct gaacaactta aattaactga
    21721 catgacactt gggagtgctg cctattttgg tgaatacttc caaaaaaaca atatcgaact
    21781 ttccatttta ggatgctata tcaacctatc cagcaaaaat caagaagttc gtgcacaagc
    21841 attggcaact ttcaaacagc aaattgctct gtgctctgcc tatcaagcca agatgattgc
    21901 tacggaaacc ggtagtgtct ctgtcggtta cacaaaagaa aattttactg aagaagctta
    21961 tcagatcgcc agaaattcta tcattgaatt agtcgctcat gcagaaaatt ttggcgtaac
    22021 tgttgctgtt gaagcaggga tcaatcatcc aatctataac tatcagttag ctaaacgttt
    22081 aatcgatgaa gtagcttcac caaatttaaa aatcatttta gattgtgcga atttgatgca
    22141 taaagaaaat catgctgatc aggaaaaagt gatccgaggt gccttggaca atttacatgg
    22201 ttatattaca gcgcttcatc tcaaagacta tatcatggaa aatggaaaga tccgcatggt
    22261 acctgttggc aagggctgtt tagatttccg cccggtactg cattatatca aatatgagcg
    22321 tcctctaatg tacgccactc ttgaagctac tcctgagatt tatgttcctg aagcaataga
    22381 acacttgcag tttatttacc aagattgtta aggagaagat tatggaggaa cgaatacaga
    22441 aaagttatca aaaatatcat gcaaatgctt ttcagacatt gctgcatctt tatcagggaa
    22501 attattggcg ttttgccatg tcaggactct tttttattat caaacatctt ccatcttggt
    22561 taatgccgat cgctattgcc aatgtaatca atgcagcttc atcgaaagat tcttctcagt
    22621 tgcaggccat ttatttcaac gcttttttga tggcttgtct gattggtcaa aatatattga
    22681 cgaattattt tcatgtaaaa taccatagca cttcgatccg tcaagtagaa gctggactaa
    22741 gagcgaccct tatcaagaaa atccaagaac tatctattcc ttatcaaaaa gaattgcaat
    22801 ctggacgaat ccagtcaaag atcatccgtg atgtagaagc tatccaaacg ttgtcctctc
    22861 aagtatttgt cagcggatta aatatcgctg tcaatttatt cgtagcttta ggtatcacgc
    22921 tttataaaag tcctgttgtt ttcatgtttt tcttattgac tgttcctctc gccgcattcg
    22981 ttactctctt tttccgtaaa aggatcagac agtccaactc agacttcagg caagaaatgg
    23041 aagagacttc tgcaaaagtg atcgaagtga tcgaattagc accgattgct cgagctcatg
    23101 ggttagaaaa acacgaaaca actcgcttaa ataagcattt tactcaaatc tatgaaaaag
    23161 gattccactt agatatcctg caatctgttt tcggatcagt cagctgggct agttttcaga
    23221 tatttcagct gatttgtttg attttcactg ggatattagc tttacaaggg cgcatccaac
    23281 ccggcgatgt cgtgatgtat cagacgtatt tcacgacgat cgtcaattcc gtttctggaa
    23341 tgatcacgct gattcctacg atttcaaaag gcatggaatc tatcacttct attggcgaac
    23401 tgttgattgc tggcgatgta gaaacttatc acggtaagaa aaaaatccaa catttggatg
    23461 gaaattttcg ttttgaggat gtttcttttc tttatccgga ctcactcaaa ccgattatcg
    23521 atcatttttc tctagaggtg aaagctggag aaacaattgc ttttgttggg ccatctggct
    23581 ctggaaaatc tacactcctt aacttattga ttggatttat ccagccgact tcaggaaaaa
    23641 tttttctaga tgatcagcct ttggatgaac tggatatgcg aagtgtgcga aattatttag
    23701 ccgttgttcc tcaaaccact ttgttgttct ccgcttcgat caaagagaac atcacttatg
    23761 gcttgaaaaa cgtatcaaaa gaacgtttag atgaagtcat cgaagctgcg caactttctt
    23821 cactgattga ccagcttcct gatggtctgg ataccttagt aggcgaacat ggcaacaagc
    23881 tgtctggtgg ccaaaagcaa cgtatctcca ttgcacgagc gttgatacgt cagccaaaaa
    23941 tcattttgct agatgaagca acctcagctt tagataacca gtcagagaaa aaaatccagc
    24001 aagccctgaa ttacctgaca gaaacaccca ctacttttat tgtggctcat cggctttcaa
    24061 cgatcaaaga ggcagataaa atcgttgtga tcgatgaagg aaaaatcgtt gaaataggta
    24121 cttacgaaaa actgttagct caaaaaggct atttctaccg cttgaaaaat gaatgacaaa
    24181 aaataggagc tatgacaaga cttttgtcgt agctcctatt tctgaataaa cggtgttctc
    24241 ctgccaactc ctcggtcaca agtcacaata gtcatcaaac atgagaagct gttttttgaa
    24301 attgttcctt gtaactcaga gttaaacggt aggctcacga cctcttggta aatggtgttc
    24361 aagagacagc ctatcggcaa taagaagcaa ttttgtcatc gctcaaggcc gaacgtctct
    24421 ttcacaccct ctgttgtttc ataaaatagt tttattcgct gtaaaggtcg cgaactggtg
    24481 tcatgatgat acggttcaag tcattgatga ttgtactgaa gccttgctct ttttgcatca
    24541 agtcattgat tacttcttct gattggattt tcacagccat ttcttgtgct ttcattgcat
    24601 cttcgtcgct gaactcttgt ccactcattt gtttttcttg taattcgatt tgcatcgctt
    24661 ggaagtcttt gaacagtttg tgtgcttctt cgtttgcttg tactttctcg tacgcttctt
    24721 tcaacgcttt gaattctggt aattcacgga tctcacgttc gatttgattc gcgctatcgt
    24781 agatgtttga catgttatgc cctcttttct tttagtagtt actctctatt gtaacatggg
    24841 gttattcgtt ttcaaagggg ctactctcct tgatatttcc gccagaggtc tttagccgct
    24901 tcaccaattc cctgaacgcc ttctttcacc atatcgccga agtttttcag cccttctttt
    24961 gcttgttcac catattgatt caagttatct tgccaatttc cagactctcc attagaattt
    25021 cctgtgtttc caacttgatc tgcagctacc acttgtcctc ctgtggcata ggcatctgct
    25081 acactaaatc ctgtttttga agtatacgga aggatgcctt ctgccgctga tttgaagact
    25141 ttcccgacaa catttccact tgtcccttcc aaatagtgca gcttgctgga ttcttcgtaa
    25201 cccatccatg tcgaaatcac tacgtctggc gtgtaaccga tcatccattg gtcatttgct
    25261 tttgtggcat cgaaattagt ttctgtggtt cctgttttcc ctgctatcgt ataacctgca
    25321 ggattcgcag cagccgctgt tccatttgaa aatgtaccaa gaagcatgct ggtcatttca
    25381 tcggctgttt cctttgatat gacctgtttt ggttttttgt ttgtgttatc gacgatcacc
    25441 gctccagtcg aatcaacgat tttcgtgata agatgcggcg tatacatctg accatcattg
    25501 gcaaatgcac tgtaagcacc cgccatgatc aatggagata ctcctttttc cagtccacca
    25561 agggctaagc cataatactt atcagatttt gctaaaggca ggccgaattc ttctgcttta
    25621 tcatatcctt tttgtaaacc gatctcatgc aaaagccata cagtaggcaa attcaaactt
    25681 tgtgcaacag cttgatacat cggtacttct ccttggtatg tcccgtcaaa attatgcgca
    25741 tcgtaataac tttgcggttc gtctttcaag atactggaag gcttgtatcc agcttccagt
    25801 gctggtgtat atacgcttaa tggtttgatc gttgatcctg gtgaacgacg catctgtgta
    25861 gcaaagctga atcctctaaa gacatgatct cctcttcgtc ctactagtgc ttgtacacca
    25921 cctgtattcg gatctaaagc tactgaagca gattggacca tggcaccatc tgaagcattt
    25981 gctgggaata atgcactgtt ttgataagta gcttctaatt gcttttgata atcctgatct
    26041 aaagctgtat agattttata gcctttattc atgacatctt cttcatcaag accatactcg
    26101 ttaactgctt catcgatcac tgcatcaaaa taataaggat aacgataacc agaatctgaa
    26161 ttatcgtatg tgtcattaag atacgcgctg atatctgtct ggctttctgc ttgttcttgt
    26221 tcttttgtga tttttccgtt ttcttccatt acacttagta ctgtgttttt ccgatcattc
    26281 gcattttttg ggtgatcgat cggattataa ataccaggac ctttcagcat tcctgccaaa
    26341 gtagcagcct cagacagcgt aacattcgtt gcatctactc caaaatactt tctggaagcg
    26401 tcttggacac cccatacccc gttgccaaaa taggcattgt taagatacat ggtcaagatt
    26461 tcttctttgc tgtacttctt ttcgatctcg attgccaaaa ataattcctt cgcttttcga
    26521 tcaaacgttt ggtctaatgt cagatacgca tttttggcaa gctgctgagt gatcgtactt
    26581 cctccgcccc ctgatgttcc gccagacaag accattcgga ctgctgcacg ggcaatccct
    26641 ttgatatcaa acccgtgatg ctcgtaaaaa ttcctatctt ctgtcgagat tactgcatct
    26701 tggatatagg gagaaatcgc gtttaattct acatatgttc ctttttgggc gtacagtgtg
    26761 ccagcttctt cgtttttctt atcataaatg actgtcgatt ggcttaatcc tgattttaac
    26821 gtttcgacat ttgcttgttt tgctaaaata aagaggtaga tacttgtcac taagacacag
    26881 atcaaaccaa tcagcaaaat caatttgttc acatgatatt ttttccagat gcgctttcgc
    26941 catcgatgga attgtccgag atatggtctg atccaacgcc aaaaactgat caaagcagct
    27001 tttatctttt ctaaaatgct ttgaaaatcc atttgcttct tctcctgtct atttgattca
    27061 atcgtcttat tttaccatag tgaaagagtg ttttcttcct gcttaagttg tagttcaata
    27121 tgttttttta cgttagtgca agtagagtaa cataaga
//
LOCUS       DAXWLG010000023        26552 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXWLG010000023 DAXWLG010000000
VERSION     DAXWLG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.023"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(172..453)
                     /locus_tag="ACGE4T_000732"
     CDS             complement(172..453)
                     /locus_tag="ACGE4T_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386037.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
     gene            complement(504..710)
                     /locus_tag="ACGE4T_000733"
     CDS             complement(504..710)
                     /locus_tag="ACGE4T_000733"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8386038.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            874..1605
                     /locus_tag="ACGE4T_000734"
     CDS             874..1605
                     /locus_tag="ACGE4T_000734"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8386039.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGNDEDLLNYLKELT
                     SFIDEIKTTTFNKKKYFQNQYDIEE"
     gene            1659..2804
                     /locus_tag="ACGE4T_000735"
     CDS             1659..2804
                     /locus_tag="ACGE4T_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8386040.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(2889..3194)
                     /locus_tag="ACGE4T_000736"
     CDS             complement(2889..3194)
                     /locus_tag="ACGE4T_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFF8386041.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(3219..3650)
                     /gene="ruvX"
                     /locus_tag="ACGE4T_000737"
     CDS             complement(3219..3650)
                     /gene="ruvX"
                     /locus_tag="ACGE4T_000737"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFF8386042.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(3647..3913)
                     /locus_tag="ACGE4T_000738"
     CDS             complement(3647..3913)
                     /locus_tag="ACGE4T_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFF8386043.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(4168..4893)
                     /locus_tag="ACGE4T_000739"
     CDS             complement(4168..4893)
                     /locus_tag="ACGE4T_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8386044.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(5254..5324)
                     /locus_tag="ACGE4T_000740"
     tRNA            complement(5254..5324)
                     /locus_tag="ACGE4T_000740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5290..5292),aa:Gly,seq:ccc)
     gene            5441..6193
                     /locus_tag="ACGE4T_000741"
     CDS             5441..6193
                     /locus_tag="ACGE4T_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8386045.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            6209..6688
                     /locus_tag="ACGE4T_000742"
     CDS             6209..6688
                     /locus_tag="ACGE4T_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8386046.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(6939..8519)
                     /locus_tag="ACGE4T_000743"
     CDS             complement(6939..8519)
                     /locus_tag="ACGE4T_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFF8386047.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(8512..9256)
                     /locus_tag="ACGE4T_000744"
                     /pseudo
     CDS             complement(8512..9256)
                     /locus_tag="ACGE4T_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(9432..10208)
                     /locus_tag="ACGE4T_000745"
     CDS             complement(9432..10208)
                     /locus_tag="ACGE4T_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8386048.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(10456..11292)
                     /locus_tag="ACGE4T_000746"
     CDS             complement(10456..11292)
                     /locus_tag="ACGE4T_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFF8386049.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(11303..12232)
                     /locus_tag="ACGE4T_000747"
     CDS             complement(11303..12232)
                     /locus_tag="ACGE4T_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFF8386050.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            12424..12777
                     /locus_tag="ACGE4T_000748"
     CDS             12424..12777
                     /locus_tag="ACGE4T_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8386051.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(12851..13507)
                     /locus_tag="ACGE4T_000749"
     CDS             complement(12851..13507)
                     /locus_tag="ACGE4T_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8386052.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            13733..15667
                     /locus_tag="ACGE4T_000750"
     CDS             13733..15667
                     /locus_tag="ACGE4T_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8386053.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(15753..16976)
                     /locus_tag="ACGE4T_000751"
     CDS             complement(15753..16976)
                     /locus_tag="ACGE4T_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8386054.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(16970..18841)
                     /locus_tag="ACGE4T_000752"
     CDS             complement(16970..18841)
                     /locus_tag="ACGE4T_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFF8386055.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(18862..19980)
                     /locus_tag="ACGE4T_000753"
     CDS             complement(18862..19980)
                     /locus_tag="ACGE4T_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8386056.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(20063..20896)
                     /locus_tag="ACGE4T_000754"
     CDS             complement(20063..20896)
                     /locus_tag="ACGE4T_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386057.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(20889..21806)
                     /locus_tag="ACGE4T_000755"
     CDS             complement(20889..21806)
                     /locus_tag="ACGE4T_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386058.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(21829..22008)
                     /locus_tag="ACGE4T_000756"
     CDS             complement(21829..22008)
                     /locus_tag="ACGE4T_000756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386059.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(22033..23157)
                     /locus_tag="ACGE4T_000757"
     CDS             complement(22033..23157)
                     /locus_tag="ACGE4T_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8386060.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            23340..24248
                     /locus_tag="ACGE4T_000758"
     CDS             23340..24248
                     /locus_tag="ACGE4T_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8386061.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            24389..26002
                     /locus_tag="ACGE4T_000759"
     CDS             24389..26002
                     /locus_tag="ACGE4T_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8386062.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8425 a         5134 c         4370 g         8623 t
ORIGIN      
        1 gtagtcaaat aaagttttct tagaacttta ttgtcgagtg tttcctgtgg tcgccaaact
       61 gtcgggaaca ctccttttaa tttttttcaa agtattttta tattttctat acttcatcat
      121 tattcaaaca aacacttcct tttttctatc aaaagcacgc cacgcaactt tttatacttc
      181 atattctttg ataaaccttg ctaattcttc ttttctaata aattgacaat ctccgattat
      241 gcttactttt aaacctttct ctctgataaa tttttttaat gtacttctgc tacagcttaa
      301 atacttacaa gcttccccca atctaaaaac ttctttgctt agagatgttt caattcgaac
      361 ttgatctact gcttcttttg caactcgata gaaatatctt cgcaattctt gatctaaatc
      421 cgatggaaga ataacttgaa tctcagattt cattttcttt gcccccacta atttgactac
      481 atgtattcaa attcaacttt tcgtcacttt tcattaaaaa aaatttgatc aattgtgata
      541 ttttcaattc ctttttcttt cacaagatac ttcaaaataa tcatttcttc tggagaatag
      601 tttgttttac cgctttcttt gtttcttacc gcttgtactg aaatgccata tttttcagca
      661 agttcagatt gtgataatcc acaaaacttt ctaaaatctt taggcttcat gactcctcac
      721 tcctccttct tttcaacttt tcgtcactaa aagaataaca tatatttatt cataccgcaa
      781 cgtaaaattc aacttttatc tactttttca tttcattata tgagaatgta gtgtaaactg
      841 tagaatagaa tagatgaagg tggtgaaacc attttgtttg ataaaaacat taattctgaa
      901 attggaaaac gaatacaaga aatacgtgtt tctttagggt atactcttga agaatttggc
      961 gaaaatctga cacctcccgc aagcaaaagc aatgtttcta gatgggaaaa aggaaagaac
     1021 atacccaaca aagcgcggct actaagtatt gctaaacttg gtggtgtttc tactgaatat
     1081 ctactgacag gtaattctca aaaaactaag aaatatatca agtatttatt caatgagatt
     1141 ttttttgaca acggtacctt ccataacaaa ttcaaagatt tcggtaaaga cagagatagg
     1201 cttggtctat atgacttatt tttagcagta tcgaaaacat atagtctaga taaatcacct
     1261 tctttaaaag ataactttgt agaaatgatt atagatgatg tatataaaaa actcttgcaa
     1321 actgataaat cagatgacca actcatcctg ggtttcatat atatttgtct tgatgaaaaa
     1381 ctttcaaaca gacagaaaag tcttaaaagt atcaattatg aggtattcaa ttatttaggt
     1441 gttgcatttg gtttaattga tagcgaaatc aaatctacaa acgcaggtaa cgatgaagat
     1501 ttattaaatt atttaaaaga attaacatct tttattgatg aaataaaaac aactacattc
     1561 aacaaaaaaa aatattttca aaatcaatat gatatagaag aataaagatt acattttcct
     1621 atcaaaagca cgccacgcaa ctgataggag gaaaaacaat ggcaacaatt aaacaatatg
     1681 aaaagaaaga cggaaccaaa gcttggatgt ttcaaacata ccttggaatc agcccgctaa
     1741 ctggtaaaga agtccgtact acacgtagaa attttaaaac aaaaaaagaa gctcaaatag
     1801 agcttaatag actacttgta gactttgaag agaatggact taaaagggaa acgaacgaaa
     1861 ctttccaaga agtgtatgat ctctggtatg aaagctataa aactactgta aaagaagtca
     1921 ctcaattaaa aattgagatc aagtttaata aatggattct tccaaaatat gggcagttta
     1981 gaattaaaga aattactaca aaacatgttc agaagatatt gaatcagtgg gctaaaacaa
     2041 cagatcaata taaagtccta cattctaccg taagccgtat tttcaaatac gcaatgactc
     2101 tagggctgat cactaaaaat ccctgtgagc tcgtaatcat gcccaacaga aatgttgaaa
     2161 aaaagaagga tactgtcaaa atttattcac gagatcaatt agaaacctta tttcaatatt
     2221 tggaatccaa aagaagtacg tatagggcag agtacgataa aactttactg cgctttctat
     2281 tttttagtgg ctgtcgaatc agtgaggctt tagccttaga ctggtcagat attgactttg
     2341 attcaaacac cgtcactata aacaaaacct taagccaaac aaaatatggg tacaaaattt
     2401 caagtccaaa aacgagaaat agctatggtt ccctatcttt agacaataaa accattaatt
     2461 ggctaaagaa atggcaaatc aatcaaaaaa gatatatgtt aaccctcggg attactgatc
     2521 caacaatgat tttttgtgga atctacaaag aaatcattac taaacacgct atctattctc
     2581 gtatgatcac aatctgcaag aattctggta ttccatttct aggtaaccat gtaaccagac
     2641 acactcatgc tagtatgctg ttagagtcgg gagtctccat gaaggaagtt caagaacgcc
     2701 ttagacactc atcaatcaaa ttaacaatgg atacctatgc tcatctaact aaagaaacca
     2761 aagaaaaaac cgttgaaaag cttgcagagc acctaaattt ctagcggaac gtattcaatc
     2821 ccgtattcaa ttcaaaacaa aaaaaagctg aacccttatc taccaagggt tcagctatca
     2881 tttacagctt attcttcttc ttccgccatg aatgtattga atacttcttc aatcatgtcc
     2941 cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc gttttcgatg
     3001 tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg atataaaaga
     3061 acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc atataatgtt
     3121 tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg
     3181 ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg cttacctgag
     3241 ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac tttttttcgt
     3301 ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac agtggttaat
     3361 cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc tccgtatgac
     3421 atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa tccaactaca
     3481 aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc aaactcttct
     3541 tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa ggggtcactg
     3601 atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat aaattcactc
     3661 catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc
     3721 gaatcaaatt tcgagcatca cgataacgag gaatgtaggc cgggtctcca gaaagcaagt
     3781 agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga taaacaatcg
     3841 ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt acggtttcat
     3901 ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt accttctaca
     3961 accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact tccgaataaa
     4021 ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca aaagtactct
     4081 tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt ttttcataat
     4141 attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt gtcctgtctc
     4201 ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt caattttttt
     4261 gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat tcccctcaaa
     4321 atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg ccgcaatatc
     4381 attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca tcaagttttt
     4441 aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat tatataacca
     4501 ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca aaggctctct
     4561 cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta ctctgttcaa
     4621 tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat cctcttttgt
     4681 aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat attctggcac
     4741 atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct gctgaaagat
     4801 atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag cagcgacgat
     4861 ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact aacagctccc
     4921 taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa acagcaaaaa
     4981 ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa
     5041 tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct tcatactcaa
     5101 gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg catctgcggt
     5161 aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt tcttattact
     5221 tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc gaacccgcga
     5281 catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca aaaacagtat
     5341 acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt atgttttgta
     5401 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
     5461 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
     5521 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
     5581 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
     5641 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
     5701 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
     5761 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
     5821 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
     5881 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
     5941 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
     6001 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
     6061 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
     6121 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
     6181 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
     6241 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
     6301 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
     6361 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
     6421 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
     6481 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
     6541 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
     6601 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
     6661 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
     6721 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
     6781 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
     6841 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
     6901 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
     6961 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
     7021 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
     7081 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
     7141 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
     7201 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
     7261 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
     7321 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
     7381 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
     7441 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
     7501 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
     7561 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
     7621 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
     7681 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
     7741 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
     7801 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
     7861 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
     7921 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
     7981 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
     8041 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
     8101 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
     8161 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
     8221 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
     8281 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
     8341 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
     8401 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
     8461 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
     8521 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
     8581 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
     8641 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
     8701 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
     8761 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
     8821 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
     8881 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt tttttttgtg
     8941 tgtctaattc ataggcagct gaaatcaagt cccaatattc tccagccgtc aattgtaaaa
     9001 aaatatctgg ggaatcaggc acataaccga ttttttgttt gatactcaat cgattttccg
     9061 tcaatttcat tccatctact aaaatcgttc ccgatgatgg ttcaataata ctaactaaac
     9121 ttttaatcgt agttgatttt ccggcaccat tatgtcctaa aaatccaaaa atttcccctt
     9181 gtcttatcga gagagacaag tcttttaatg cttgtttttt cccgtaactt ttacttacat
     9241 tgatgaattc aatcatttct gtactccttt tgtttgttat tactttttat tgtacccttc
     9301 gttattggtg gagtaaatgt caaaaagagt gcataacaaa aaaggataga aacgactagg
     9361 ctgctacttg ggttgttctc ttctacagga aaacactctt ctaacaggta agatatgagt
     9421 ctttgttttc tttaaaatga ttcattttct atcttttctt gcataaaatt ctttagttta
     9481 tcaatacttc tctttcccat ctctagttgt tctccatttg caaatgttac tatttcgttt
     9541 actctgctaa tagatgtaat gttttcagta ttaatgatat aagcttttaa atctttacat
     9601 aaatactcat attcgagtga ttttttatat tttttaattg tgccgtttac gagaatctct
     9661 tcatctttca atactaggcc tattttattt ttataacctg gcatgctcat tagataaagg
     9721 atttcatcgt aatggatata gtgatcgcta ttaccataac ggacagttgt ccaccttttt
     9781 ttcgtagcat gttgaacagg atagttttct ttttctttca ataagtaact ttggatgctt
     9841 tttttatttt tttcataagt gaattctttt atcaaataac cactaggcgt aatctttcga
     9901 ttaataacag ttattgcttt attcggatga gacgttagat aaacaatttt acacttttca
     9961 ttcttttctc taagcatttc gcctagttcg ataccgctat aataggcgtt caaatcgatg
    10021 tctaggaaaa aaatatctga ggaatagatt tcattatttt taatgaattc aacaaattgt
    10081 ataacgttca atccctgaac gatctcaaat ttccaaggac ttaataaaat tctggattct
    10141 tccaaaattt ctactagcat tttttcaaaa accaaactat catccaaaat aaatatacgc
    10201 gttaccataa aaagtacctc cactcgatat tttactatca aaatcatttc atacaacttt
    10261 tcaacataca tgttaggaat aaattgtaat tctcttcaca atcttttcca acacctttga
    10321 aaccgatatc aaatactttc aggatgctgc ttctaaaata aatatcaaac aaatgaatac
    10381 atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg agctatgttt
    10441 atttattttg ttcttttaaa aagaacgata cacatactga tttttcggag cagagacttt
    10501 ctcagcgttt gtaacaacta cagatgggat ttcacgcttg aatggttgat aatagtctga
    10561 ttggatggtt ccttctattt tataccattc atcattttta accgttactc cttccggcat
    10621 atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa tggataatgc cgaatcggaa
    10681 caagaagata tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt catagctgat
    10741 ttttttgcca atgaattcgc tagggtaatt ataaattagc tccataactt ctaaatagtt
    10801 ttcatctgtg atcttgatag tttgcttatc cccatacttt tctttcaact cattcatttg
    10861 gtctaagtag tctgatttgt tgaaataaat actggtatcc ggctttaagt attgtgtctg
    10921 catctccgga tcccctactg attctttact tactgggaaa ttaaatcctt tggcttctac
    10981 gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc acgattactg gcaaagctag
    11041 cagtacataa gcgattccac gttgataagg ttttgacaac ccatggtcat gatcgtgctc
    11101 gtgtgccttt tcttctgctt ttccgttttt gacccacaaa tacagctgaa ctaaagccaa
    11161 aagagcagat aaaatcattg aaagaatagc taagtagcga taatggacat tgatatattg
    11221 atcgagtttg ccagaaactt gcaagtacat catcaattcg aaatacccag ataaaatcaa
    11281 aaaccgcagc atattttccc tcctaaataa ataatgtgaa tccaataacg ctaagggcaa
    11341 tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc atcagtagat
    11401 ttttgatatc gaccattggg ccaaagacca aaaaggcagc tacaggtgct gtgccgaaca
    11461 gacttaacaa agaagatcct ataaacgcat ctgcttctga gcaaagagac atcgtaaagg
    11521 ccaataacag catgatgata atggctaaaa tctttgttga gccgagcgtt agcataattc
    11581 tagttggtaa ataagtctgc atcgcagctg cgattaatcc gccaatgatc aaataacgac
    11641 ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg cccagtttga tggatcagct
    11701 gattttttgg ttcattcttc tcactttgtt cttcttcatg gacatgatga tggtgatttt
    11761 ctgctacttt agacgtgatt ttttctttca atacagcgtc tttttgaaaa taggcaagcc
    11821 agacaccaat cacaagggca acaaaaaagc tgcctatcac ccgccagctt gccatctgcc
    11881 aagaattgcc aaatgcgata aaagtggaaa aaatcacgat tggattgatg atcggagcag
    11941 tcagcataaa agcaaacgct gtgtgaactg gtacgccttt tttaacaaat tgatgaacga
    12001 tcggtacgat gccgcattca catgaaggaa agaaaaatcc taagatcgaa ccagtaaaaa
    12061 tagagaagat acgattttca ggaagccatc tcttcacacg ttctggggtc aaaaatactt
    12121 gtaaggcgcc tgagataaga cagcctaata atacgaaagg taatgcttca ataacgatcg
    12181 ataagaaaat cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac atgagtttcc
    12241 tcctttttat ttctagttca ttcgctttgc aaatatacgc ttttattcaa aaaaaacaag
    12301 ttgctctgta aatttttaca caactcttat tttttaccaa agcacttcaa ttgacttttt
    12361 ctgttattcc cattaaatta aggtctcata catatcactt ctatcataaa agggagaata
    12421 aaaatgaaac aaaccactaa cttgactgaa gttcaagaca tactgcaaca gtcatccgtt
    12481 gccgtcattt atctttcgat gcctaattgc agtgtttgtc acgctgttcg tccacgacta
    12541 gaggaacttc tcacacatta tgatattccg gcacttcatt tggatgcatt tgaaacacca
    12601 gaagtagcta gtaaatttga agtgctgact gcgccagttg tcctgatttt tcatcaagga
    12661 aaagaagtct ttcggcaagc acggttcatc gactttaaaa aaatccgtta tctactagat
    12721 gaactgacag cagaagatga ttccctttct tatgaagaaa tcttccgaac aaaataatcg
    12781 ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg
    12841 ctttttattt ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat
    12901 agatcaaggt ttgcaattca ttcgtcaaat caacattttg gatcaataca tctgacggag
    12961 cattcaatcg agcagcggta aagttcaaga ttcctttgat tcctgcttcc gttaactgat
    13021 tgactacttc ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg atttgttgga
    13081 tcttgatttg ttctgacata tcttccattg gataaacggg gataccgtct acgatgcgcc
    13141 caacgatttc ctcattcaca tcaaaagcac aactgatacg tatgctatta ctttgatgga
    13201 atttgtattt tagtaaagca cttcccaagt tccctacacc gatcagcgca acatttgtca
    13261 attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag atcttctaca tcataaccat
    13321 acccacgttt tcctaattcg ccaaaataag aaaaatctcg acggatcgtt gcactatcta
    13381 cttgaacggc ttcacttaat tcagttgaag acactttttt ctttccagca ttatttaaaa
    13441 tccttaaata gcgataatac aaaggcagtc ttttagctgt cgcctttgga ataatatgat
    13501 ctttcacgaa atgacctcca cgtattgtct aattacatct ttgatttctc catcatagca
    13561 gtatttatag gaaaaaatca attccattgc tcagatattg tgaaatttta ttctttattc
    13621 atttatttat catttctgca aatctcgcag tataatggaa tttatggtag actactaatt
    13681 gaaaaatagt tttttatttg aggagaattc acaaagtaga aaagaggatt ccatgatatt
    13741 attacaagcc aatcagattg ctcgccattt tggtgcagaa gtcttatttg aaaacataca
    13801 tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc
    13861 gactttatta aagatcattg ctggaattga agcacctgat gaaggaaaga tcgctaaaaa
    13921 taaaacagcg actctcggat acctcgctca ggacacagga cttgattcca atgaaacagt
    13981 ttggaacgag atgctgaaag cttttgaaca agttagacaa atggaagagc ggatgcgcga
    14041 gctagagatt ttcattagcg aaggtacgcc agacacacct gcttatagtt cttatttaaa
    14101 agaatacgac cggctgcaac acgatttttc agatcgaaac ggctatggtt atgaaaatga
    14161 aattcgttcc gtattgcatg ggtttaaatt cgattcttca ttttatgatc agaaaatcga
    14221 tactttatca ggcggacagc gcacacgttt agctttagct cgcatgcttt tgcaacaccc
    14281 tgacattttg attctagatg aaccaacgaa ccatttagat atcgagacac tttcctggct
    14341 tgagaattat ctgcaaagct atactggtgc attattgatt gtttcccatg accgttattt
    14401 tttagataaa gtagtcaatg aagtctatga attaagcaga agaaaaatgc gccattacaa
    14461 aggtaactat tcgaaatatc tagatttgaa agcagaacag ttagcaagtg aatggaaggc
    14521 ttatgaaaag cagcaaacag aaatccataa attagaagac ttcgttgctc ggaaccttgt
    14581 tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag acattagaaa aaatggaccg
    14641 gctggatcgt cctcaagggg atgaaaaaca agcaaacttt ctattcagta tagaaaagcc
    14701 ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc ggatatgata atgtcacctt
    14761 atctgaacca atcaatctag atatccgtcg tcaagaagcg attgctctag taggtccgaa
    14821 tggtatcggg aaatcgacgt tgctgaaatc aatcattcac cagatccctt ttatccgtgg
    14881 aaaagaaacg ctagggacaa atgtcaccat cggctattac gatcaaggtc aagctgatct
    14941 gcatacaaac aagacgatcc tagcagaatt gtgggatgag catcctacca ctccagaaaa
    15001 agagatccga agtgctttag gtgctttctt attttctggc gaagatgtag aaaaaccaat
    15061 ccctctgcta agtggcggtg aaaaagcacg ggttgcttta gccaagttag cgatgaacaa
    15121 agaaaatttt ttgattttgg atgaaccaac aaaccattta gatattgaca acaaagaagt
    15181 attagaaaat gcattgatcg attatgacgg cacactgctt tttgtttccc atgaccgata
    15241 tttcatcaat cggatcgcga caaaagtcgt cgaactctct gaaaatggca gcaaagtcta
    15301 tctaggagac tatgattatt atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt
    15361 gttggcacaa gatgagccag aatctgctcc tgctcccacg aacaaatctg attattacca
    15421 aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga aaaattactc aggttgaaga
    15481 agaaatggcg cggatcgatg aatggatcga tcagttggaa caagaaatgg tcgcgccaga
    15541 aaacctggaa gatcacgtaa aactcaatga gctgaaccaa gatttagaag ctgctagaca
    15601 agatcaagaa acaaaattga cggaatggga agaactaagc ttggaattag aagaatatga
    15661 aaattaacga aaaaaccaaa acagaaacca ttcatgctgt tttggttttt ctttttttat
    15721 tttatcaaac aaaaattcca tatttacctt tttcaccaat aagaagacca attactagtc
    15781 attctcacta aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt atttacccca
    15841 ttatttttgg gcttattata aacagactgt gttaaaaagc catcttcttc ttccgttgta
    15901 gcatatcttt ctttcaattg tcgcataatt ttccaaacag cagactggta taagttcttt
    15961 tctgttttgt ctaatagacc tgattctatt aattctaata atccacaaac catgatcacc
    16021 atactagatg tatctttttc ttctctgcta gaatcatcaa agatcaaatc ccaataacaa
    16081 atggtatctt ctggcaaccg attcataaaa taattggcta atattttgct taaatcaaaa
    16141 tatttttgtt ctttcaaata acgataactt aatgcaaaac cgtaaatccc ccatgcttgg
    16201 cctctagccc aacaggaatt atcactataa ccttgatgcg tacttccata tcttggtttt
    16261 ccatctttga catccatata aaatgtatga taagttgaac catcttctct tactaaataa
    16321 tctgctgcat tttgaatatg agcctgagca gcttttttat atttttgatt tccagtttct
    16381 tcactcgccc aatacaataa tggaagattc atgcaacaat ccatgatcat tctaccttgt
    16441 tgtgaaggat cactaggatc tccccaagct tgtataactc ctgccttctc ccaaaaacgc
    16501 tgaagtaatg tttctgcagc atcaattgaa gcttccttca tttttcgatt tcccgttatt
    16561 ttataatctg ctacggttga taaagaatac aaaaaaccaa gatcgtgatg attagtgata
    16621 atttgacagt caatcctgtg gataaaatct atatctgctt tttcagctgt ttgttgaaat
    16681 aaaggatcac ccgtcttctc aaataacaac caattaattc ctgtccaaaa tccttctgtc
    16741 caaccaatat tgtctcttaa tggataacgt ccttttatag gacactcgct tgggaattga
    16801 tactgactgt tactgatacg tttgattaac ctctccgtgt gtctgcgaca acgctcgatc
    16861 tcattattcc attctttttc cattaattct ttataacatt catttatcca tttttctgga
    16921 tgtattatct cttcaaacat cactttatca agcatttccc caccagcctt tagcataatt
    16981 ttagtctttt tttattgttt ttattttgga tagttagccc ggttgtccca tcaaaatcaa
    17041 ttttgggacg attgttccat acttctaaac tataatcccg atcttgatga gcaaccactc
    17101 ctgttccgag ccaatgggtt ccttttccaa atgtaccctc taatccagga ataaaagtgt
    17161 tttcccatgt catcaagttt gtattgggca aacccataat agctgtggat tttcgtttaa
    17221 tagtatcgtc acttaaacag ataatacctg aaaagccagc ttcactcacc ctatagtttt
    17281 ccttgtcttc ttctatgatt attccttctt tctcaggaga acattcaata gcaaaccctg
    17341 tctcatatag aacatattct gtatctaatt ctactttgtg tacgcgaatc tgccagcctt
    17401 caaaaggaat aagccaagtt ctgatttttg cctgtaaagt aggttcccat gtagaggcaa
    17461 gtacctcttt cttacataca atgtctcgta cattatttcg aacgatgaac tgtccatagg
    17521 ttttgatgct ctcattctta aaactggtcg tgatatatgt gtccgctcgc tgaatcccta
    17581 gcattgaatc cattgccatt tctgctttca attgattcgc tcgcggtatc gaaaaaccaa
    17641 attgactaga ataagcaaat tttaaatatt tttcattggt caatgtatgg aaatttggcc
    17701 ctaattgtcc cgcattcaaa aaaaatgtat gtccattatc atgttgtatg agcaaattgg
    17761 cttctgaaag aagtttagtt ccttcacgaa taggcatagg catttcttta gccgtccaat
    17821 actcatggtc ttcaggtagc gccaacaata aaaaaatctt attcagccat aatggagata
    17881 acgtactatt atagggttca gtcaatgtca attgagggta ggtataacca atattcagga
    17941 tacctttttc atcaaatatt tctttttgca tccaccatct tatattccga ttgactaacc
    18001 ccttgatttc acctaattta aaaggcaaaa gatcggccca caacatagct gaccaaaagc
    18061 ttgaaactgc aaaacgataa atcatgcttc gaccaaaagg cacatttgca ccatcttccg
    18121 caaaaaaatg aatatagtct ctagaaaaat caatagctcg gttcttatat atctggctaa
    18181 tgggcttttc tggttctagt acgctataaa tcaaaccata ataatgaaat gcaaaaggat
    18241 tataataatc ttggcgacct cttgaactgt ctcgatacca gccattttga agataacaat
    18301 tttctatatt atcaaatgct tctttcagtg ctgattcttt aactggataa ccaaattttt
    18361 ggataattgt tccgacaatt atcttaaaaa attgccaatt accatctgca cattcatatt
    18421 caaaaatttg cgagaaccaa tgaataatct ggcttttttc tcctgtatct aattttttcc
    18481 ataataattg ttcatgatga tataacgcta ataacattgc gggcatttct accatacgct
    18541 ggtctctttc atgtatttct ccccaataat cgggatggga aggatctgtt ccagctttta
    18601 ttcctgatag aattaatttc aaccatcgtt catctaattg gtcaattgcg ggccccgctc
    18661 cccataaaac ccgagagaat ccttccattc ctgcaatttt atttccatat cctacccctt
    18721 gatgtatata atcaatttgt gcgccacttg ctcgataaaa cggctggagt ggttgattta
    18781 tctttttaaa taagtcaaag taatcctgtc tggtccttaa tagtggtact ggatgtttca
    18841 ttgtattttc tcctcttttt actaccaaat agctaattcc gactgttcta atcgaagtaa
    18901 gccttcaata aaaaaataat cagaatagat cagtggcaca ttataagtca gttctctatg
    18961 gtatgcttct gctccgcctc caatgatccc atcttcatcc atattccaat ttgcatagtg
    19021 cttttctgtc ttttggagta tattgatagc agcttcatga tacatttttg atcgtacaga
    19081 ggaaacatat cctgctaatt ccagaagacc gcatgcagcg catactccag cactggtatc
    19141 aatattcgaa tcttttacag caaaatcaat gagaggaata taaccagttt tttcgatttt
    19201 ctgaataaaa tttactgcta tcttttctgc tatagctaga tagtacggat tttttgtgta
    19261 acgataactt aacgcgaatc catataaagc ccatgcattt cctctgctcc aacaagaatt
    19321 ctcattgtat ccttgcccgc caagtaaccg aatatatttc cctgattctg catcaaattc
    19381 tgcgatatgc ccggttgatc catctttcct cactagatac ttagctgttg tatctgcatg
    19441 ctgccgagca attttagaga acctcggatc accagtttcc ctcgaagccc aatacagtaa
    19501 tggtaaattc atcatactat caataatcat ccaaccaggt ttatggtcat tccatgctac
    19561 taaatagttc cctgaaatat tatagcgtcc agcaagtacc gtagctgcat gtaaccctag
    19621 ttgtcgacta tgtttgtttt gagactgttg ataatgtgca atagccgatg gtaagaataa
    19681 aaaacctaca tcatgatgaa cgtctgaaaa atggtacagg gcagattcta ataattgttc
    19741 ttgttgtatt gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa
    19801 aataccactc caaaaaccat tggtccacca gtcgattcct ctgtccttta ttacgtctgt
    19861 atattttccg ttgtaagaaa tatatggaat tttattcccc attctgtcta attccgcatc
    19921 tatcttttca gaagctacat tcaaaatttt tcttgaccat tccttcgtct gttcattcat
    19981 ggatatcccg ccattctttt aatttttatg aatgtggagt tgctcctaga acgaactaga
    20041 tggttaacat tcgttatttt ttttacttat ttgcagctcc aatgtcattc tccttaaatt
    20101 tagactgcgc ccacacataa atcaagacca ttgggatcac tgaagttgct aaaattagca
    20161 taattgcgcc ataattaatt ggctcacctg tactagaaac cgtcatattt ttgataatta
    20221 agggaaccgt atatttatct ttatctgaaa gcatgactaa tggaaataga tattgattcc
    20281 aacttcccat aaagataagt aaaccactag taataatcgt aggtgataaa atcggtacag
    20341 cgattttcgt aaaaatcacc cactcattgg caccatcgat tctggcagcc tccatcagtg
    20401 ctgtaggaaa ttgactgcta tattgtcgca tcataaacac tgaagttgta ctggcaatcg
    20461 aagggatgat cacacctgca taagtattcg ataatcctaa acgactcata atgtaaaata
    20521 aaggaatcaa tgtggtaaaa aaaggcacca tcctagatag cataactgta ttgaacaaca
    20581 gtttattccc cataaaacga tactttgcta atgcataacc cgccattgta gaactggctg
    20641 tagctaaaac tgtactgatt acagcaataa caatcgaatt ccaaataatc aagggataat
    20701 tatatctcgc catgatggct tgccagtttt ttaatagcat atccccaaac ttgaaattga
    20761 ttccgccact taaaatttct tgattacttt gcgttgaagc tacaatcata tataaatatg
    20821 gcatcaaaaa tattaaggca ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta
    20881 tcttttgttt agtcattttt atcacctccg aatttaaatt gaatagccga taatataaca
    20941 gatacaaaca ccatcgtcca ggcaatagca gaggcttttc caaaatcaac catttcaaaa
    21001 cttgttttat ataaattgac tcctacgacc aaagctgaat tttctggtcc attggaagtc
    21061 ccaaagaata tatttgggat attgaaatta cccaagccat tgatagtggc taaaactgtg
    21121 gccattaaaa tgattggctt tagcatgggg atcgttaata aaaagaattg ccgaaatttc
    21181 gatgctccat ctatttcagc actttcgtat atctctttag aaatcccttg aagaccagct
    21241 aaaaataaaa tagtgttata tcctacacta agccaaatca tcgtgatgat cattgatatt
    21301 cgtatccaaa aaggatttgt taaccactgt acaccgttaa ttcctagcgc attcaaaaat
    21361 ccattgaata acccagtcgg attgaaaaaa gtcatgaata cggaagctac agccaccgtt
    21421 gatgtaatgg ttggaatata atagacagtt ctaaaaaatc cttttccttt taactgtgct
    21481 gaattcaata aaacagcaaa gtaaatagct aatagcatcg ttattggaat tgatcctgcc
    21541 atgataatca aaacattcgt gtaagattta ataaacaatg gatctcggaa gatagctaga
    21601 taattgtcaa atccaacaaa ttcaaatgag tagccaattt tcataaaact aaaagcaaac
    21661 gagataatga tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg tgtgaccaat
    21721 ccataagcac tttttttatg agacgtatct gttgcttttt tcttgctgtt ctttattgtc
    21781 ttgcttggta acgaagattg tatcatcgtt aagtcctcct aatatttttt aatcaccagt
    21841 taattttacg ccgtacttat gagcaaaatt ttctgcttct tttagatatg aattttcaaa
    21901 atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa taagacgtag catctgaata
    21961 agtggctggt aaaacagtat ttgatgtatt ttcattgatt ttattcaata gttcatagta
    22021 tttttgtcct ccttagtagt caaacttttt ctcagctgct tctgtttgat aggccaatga
    22081 gttagatgta gccacgccca gctccagtgc gttggctaag cattcttcat ctgtcagcat
    22141 aaatgtaaga aattgttgtg ccacttgtgg atttttggca tctttaggga cataaaaact
    22201 tgatcctcct gatactgcac cttttcctgg attttctttt gaaaaaggaa tcagtggagc
    22261 catgcgccat ttgtcagatg catcaggaaa attcaaagac atattcgtag cataccagcc
    22321 gccagcaaca aacattgcag attcttgaaa tgctttctca gcatcttgtg caccgtagaa
    22381 atgtacaata tctgagtcga tcatttcttt gattaacttt gcagcatgtt tcgcttcttt
    22441 agtattcaga cggatatttc cttcttcatc aagcaaagat tgattctgct gggctagcat
    22501 gttgataaac aattcttggt ccccagattc tggcaaggca attccaaaac taccagtttt
    22561 ttcttttaat gcttttgcca cctggatata ttcttcccaa gaactgattt cttcaaaatc
    22621 tacacctatt tcagcaaatt tttttggttg ataataacaa agtgctgggg taaaatcact
    22681 agcccaagga ataatcgttt cattactcgt ttgttgacgc aaaatattca atttttgatc
    22741 ataaaactga tcgccatatt tgtcataaaa tccatattca tcagcactat aaaaagcatc
    22801 ttgaaatttt tccagcacac ctgctgctga agtgtctgcc atggagataa tatccggcat
    22861 atcttcgtgt gttaccaatt ttggggtgat catttgtact gttgctgttc ccgaagctac
    22921 attcacaatt tttaagtcaa tatttgctcc agttttgcgg ttatagattt ttgcagcatt
    22981 ttcaacatag tttgtcagtt tgccgtttgc gacatatact tctaatgtag tggctttttt
    23041 tatcattgga tctgcgatgt ttgctgttac ttcttcgtta gtttttccac atgcacttaa
    23101 gctgaataat agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt ttttcatgtc
    23161 tatcccttct ttcttttcaa gttcttatat aaaacgcttt cacttatgta tattaacgga
    23221 attagtaaca caaatattta tataaaaaaa tattttactt gtcgttttgg cttttcattt
    23281 ttgttataat agtcattaat tataaataga aagcttttta tataaaaaga gaaaggaaag
    23341 tggttctttt gttaacaaac aaccaatcct ccaatcttgt caaaaaaaca ctaacctatt
    23401 ccatagatac ccatcttcat ttatctgctg ctccctattt tcgactacta ataaatgaaa
    23461 gcgctaattt atatgctgaa tttgcacata aatctataga actagataat ccttcccttt
    23521 tattcattga taaacagcaa gaagtccaaa tcttttctac tcaaaaggaa acacaaactt
    23581 ttatcattat ccagttttat tttgatgagc aattattagc acatattacg ggacaatctt
    23641 tttctataga tcaagaaata acatctatca ttacttttct gaaaaatgaa cagttcaaaa
    23701 ttgattatct aaaacatagt aagccagcta tttcctatga tcaggaaatc aattcttttt
    23761 tcatactatg ccaacagatg agcattcatt tgctttcctt attagttact tattgtctgc
    23821 aaaaaattca attgtcttcc ttaccaaggc attatcaaca ggaacttgct ttattgacaa
    23881 taccttcaaa tgagaaagtc attcctaaaa aaaagcatat tctttattca gatgaattag
    23941 ttgatccaat catacgatat ttagaagaac atctttcaga aaattttttg cttgaacaat
    24001 tagcccaaat tttttttatc agtccagcca gcttaaaaaa acattttaga aaagtgaatg
    24061 gaaaaagtgt tattagctat tttaaagaac taaaaatgga aaaagcaaaa cgtctcgtgc
    24121 tagaaaatga attaacctat acagaaattg cagatatatt aggatttcat tcgatccatc
    24181 atttttcttc agcttttaaa aaatatacag ggttatcccc ttcaaagtat tatctatcct
    24241 taaaatagaa aaaaattttt aggttttatc cacaaattat ttctctatat tttctgtcta
    24301 ttcaaaataa acaataattt tccggcacaa ttatttgctg ttttttacca taacatagta
    24361 cgatcaacgt agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg
    24421 cggtccttca tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta
    24481 tgaacgcgga gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt
    24541 gatcactgac cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa
    24601 catcgatgtc tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac
    24661 agttaaagac ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc
    24721 ttctggtgct agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt
    24781 tgtcctccgt agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc
    24841 acaacatgtc gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa
    24901 gcatcgcggc ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga
    24961 tccagaaatt gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt
    25021 agaaacaaga gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc
    25081 cgtcctttct gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt
    25141 gaaagcaacc ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca
    25201 aaccaatctg ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac
    25261 tggtcagccg atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc
    25321 agatatttta aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa
    25381 aatctttgat catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg
    25441 cataacaaat tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga
    25501 agctacaaga ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg
    25561 acaagcattt ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac
    25621 cggaaacttg acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc
    25681 tactacccgt gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat
    25741 cgtagaaacg atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt
    25801 agatatacgt gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc
    25861 aatgaatgag ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac
    25921 tttcagaaga ggattgaata cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa
    25981 agcgattttg attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta
    26041 tttttacttg aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag
    26101 aatagaagat aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt
    26161 taagaaagtt taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac
    26221 atgttgtgac tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta
    26281 atattacgag gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga
    26341 atattttttc ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg
    26401 cttgcgttgt gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata
    26461 tttttcgcca tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta
    26521 aataatttaa tttttttgtt cctatttatt ta
//
LOCUS       DAXWLG010000024        24359 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXWLG010000024 DAXWLG010000000
VERSION     DAXWLG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.024"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(216..1511)
                     /gene="purB"
                     /locus_tag="ACGE4T_000760"
     CDS             complement(216..1511)
                     /gene="purB"
                     /locus_tag="ACGE4T_000760"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFF8386063.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1524..2648)
                     /gene="purK"
                     /locus_tag="ACGE4T_000761"
     CDS             complement(1524..2648)
                     /gene="purK"
                     /locus_tag="ACGE4T_000761"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFF8386064.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2641..3129)
                     /gene="purE"
                     /locus_tag="ACGE4T_000762"
     CDS             complement(2641..3129)
                     /gene="purE"
                     /locus_tag="ACGE4T_000762"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFF8386065.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3545..4852)
                     /locus_tag="ACGE4T_000763"
     CDS             complement(3545..4852)
                     /locus_tag="ACGE4T_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFF8386066.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4855..5436)
                     /locus_tag="ACGE4T_000764"
     CDS             complement(4855..5436)
                     /locus_tag="ACGE4T_000764"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFF8386067.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5552..5649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6008..7300)
                     /locus_tag="ACGE4T_000765"
     CDS             complement(6008..7300)
                     /locus_tag="ACGE4T_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8386068.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7297..7920)
                     /locus_tag="ACGE4T_000766"
     CDS             complement(7297..7920)
                     /locus_tag="ACGE4T_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386069.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7923..8759)
                     /locus_tag="ACGE4T_000767"
     CDS             complement(7923..8759)
                     /locus_tag="ACGE4T_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386070.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8776..9663)
                     /locus_tag="ACGE4T_000768"
     CDS             complement(8776..9663)
                     /locus_tag="ACGE4T_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386071.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9679..10962)
                     /locus_tag="ACGE4T_000769"
     CDS             complement(9679..10962)
                     /locus_tag="ACGE4T_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8386072.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRIKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11645..12328)
                     /locus_tag="ACGE4T_000770"
     CDS             complement(11645..12328)
                     /locus_tag="ACGE4T_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFF8386073.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12346..12963)
                     /locus_tag="ACGE4T_000771"
     CDS             complement(12346..12963)
                     /locus_tag="ACGE4T_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8386074.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12973..13797)
                     /locus_tag="ACGE4T_000772"
     CDS             complement(12973..13797)
                     /locus_tag="ACGE4T_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFF8386075.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGTKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13790..14350)
                     /locus_tag="ACGE4T_000773"
     CDS             complement(13790..14350)
                     /locus_tag="ACGE4T_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386076.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14524..15480
                     /locus_tag="ACGE4T_000774"
     CDS             14524..15480
                     /locus_tag="ACGE4T_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8386077.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15785..16741)
                     /locus_tag="ACGE4T_000775"
     CDS             complement(15785..16741)
                     /locus_tag="ACGE4T_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8386078.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16845..17447
                     /locus_tag="ACGE4T_000776"
     CDS             16845..17447
                     /locus_tag="ACGE4T_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8386079.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17535..19034)
                     /locus_tag="ACGE4T_000777"
     CDS             complement(17535..19034)
                     /locus_tag="ACGE4T_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFF8386080.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19050..19574)
                     /locus_tag="ACGE4T_000778"
     CDS             complement(19050..19574)
                     /locus_tag="ACGE4T_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFF8386081.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19872..20621
                     /locus_tag="ACGE4T_000779"
     CDS             19872..20621
                     /locus_tag="ACGE4T_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8386082.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20612..21397
                     /locus_tag="ACGE4T_000780"
     CDS             20612..21397
                     /locus_tag="ACGE4T_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8386083.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21366..22082
                     /locus_tag="ACGE4T_000781"
     CDS             21366..22082
                     /locus_tag="ACGE4T_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8386084.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22184..23203)
                     /locus_tag="ACGE4T_000782"
     CDS             complement(22184..23203)
                     /locus_tag="ACGE4T_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFF8386085.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23222..24223)
                     /locus_tag="ACGE4T_000783"
     CDS             complement(23222..24223)
                     /locus_tag="ACGE4T_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFF8386086.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7206 a         5256 c         4257 g         7640 t
ORIGIN      
        1 tattcttcat cgatcattaa cagacaaaga actggcccgc caaggatatg aggacatctc
       61 cttgaagtac cagttcgtac actcaactta ttgaaccgcc gtatacgggt ccgtacgtac
      121 ggtggtgtga gaggggcgga aggtcagttg gctttcccct ctactcgatt atttttgcta
      181 ttctatttta attgaatttt gcaactcact cttctttatt ccaaacctac tcgtttgaat
      241 atcacgtcaa cgtttttcaa atgatgatga taatcaaagg catcgtctat ttcttcttta
      301 gataataccg atgtgatggt tggatcttct tctaacagcg ggcggaactg cacttggttg
      361 tcccaagcgt acgcagtttt tggctgaaca agatcataag cttcttctct tgtcattccc
      421 ttatcgatca atttcagcat aacgcgctgg ctataaatca aaccaaatgt tgcagacata
      481 ttccgcttca tgttttcagg aaagaccgtt agatttttaa cgatattgcc aaaacgattt
      541 aacatgtaat tcaataaaat cgtggtatca ggcaagatga ttcgttctgc tgaagaatga
      601 gaaatatctc tttcatgcca taatgtgaca ttttcatagg cagtgaccat gtgtccgcgc
      661 accactctgg ctagaccagc catattttct gaaccgatcg gattcctttt gtgcggcata
      721 gcagaagaac ctttttgacc tttcgcaaaa aattcttcta cttcacgtgt ttcggatttt
      781 tgcaatccgc gtatctctgt tgcaaaacgt tcaatacttg tagcaataag cgccatcgct
      841 gagaaatatt cagcatgtag atcccgaggg agaacttgag tagagatttc ttgtggacgg
      901 atccccaatt gtttacatac atactcttca ataaatggcg ggatatttgc gaacgttccc
      961 actgctcctg agatttttcc tgcttctact ccttttgccg catgttcaaa tcgttcgatg
     1021 ttccgtttca tttctgaata ccatgtggca agtttcaatc caaaagtagt tggttcagca
     1081 tggacaccgt gtgtccgacc catcatcact gtatatttgt gttcttttgc tttttcgcca
     1141 atgatttccg taaaacgttt caagtcttcc cgtaaaatat catttgcttg cttgatgaga
     1201 tagccataag cagtatctac gacatccgta cttgttagac cataatggac ccattttctt
     1261 tcttctccca atgtttcaga aaccgctcgt gtaaacgcaa cgacatcatg gcgtgttaat
     1321 ttttcgattt ccaagatccg atcgatatca aatgatgcat gctcacgaat ttttttcaca
     1381 tcttcttgcg ggatttcacc aagttctgcc caagcttcat ctgctaaaat ctcgacttcg
     1441 agccacgcac ggtatttgtt ctcttccgtc cagatattcc ccatttcagg gcgtgtataa
     1501 cgttctaaca tgttttttct cctctaatcc caaatattcg tttgtttgat ttcttccagc
     1561 gtttgctgga tgtcttctgt caagatagtg atatgtccca tcttgcgacc tttttttgct
     1621 tgtttttttc catagaaatg gaattgccag tctggcttat tctcaatcaa gcgatagctt
     1681 tccagcagtt gttctcccaa aacattgacc attactgcat tgcttatcaa acgtactttt
     1741 tctcctaatg gccaaccaca aatacctcga atatgggcat cgaactgact catagaacaa
     1801 gcttcaatag aataatgtcc agaattgtga ggacgaggag ctaattcatt cacatatagc
     1861 ccgcctgttt ttgttaaaaa catttctacc cctaaaacac ctgaaaggct cactgcttct
     1921 gcaatcacac gggcaatacg ctcagcctcc tcgatcacat cctgatcaat cgcggctgga
     1981 gcaatcgttt catgaagaat attgttgtgg tggatgtttt ccacgaccgg aaaagtcgtg
     2041 aaatctcctt gaccatttcc tgccaccatg atcgaaatct ctttttcaaa cgggatccac
     2101 gcttctaaaa cacaggttcc ttctcttaaa aggtccatgg caggagctag atccgcccga
     2161 cttttcaaaa catactgccc cttgccatca tagccgcctc gagtggtttt caaaacacaa
     2221 ggatagccga tcccgtcaat cgcatcttgg atatccgtcg gactgacgat cgttgcataa
     2281 ggagcaatca cgatgttatt cgtttccaaa aaagattttt ctaacaggcg gtcttgcgta
     2341 atcgcaagca agtctgtccc ttgaggaata aatgacatgg gtaaaatcgc atttaacgcc
     2401 tctacactga cgttttcgaa ttcataggta atgacatctg ttcttctggc aagctctttc
     2461 agcgcaaagg tgtcatcata gacactttca atgtgccaat ctgcaacttg tgcggctgga
     2521 caatcgctca caggatccag cacacctact ttaaagccca tttcttttgc actaatcgcc
     2581 atcatacgac ctaattgacc gccaccgacg atcccgatcg ttgaaccagg caacaaaggt
     2641 ttaaccaagt tgatcactac tttctataac agttttagcc aacattgaac gtctttcagc
     2701 caatttagcc tccaattcta aatcatacat ggaaagcatc tgtatcgcaa gtagtccagc
     2761 attaattgct cccgcttttc cgattgctgt agtagctaca ggtactcctc caggcatttg
     2821 gacaatcgaa agaagcgaat ccaaaccatt taatgttcgt gattggaccg gtacaccgat
     2881 aaccggcaaa gttgtttttg atgcgaccat acccggcaga tgcgcggctc cccctgctcc
     2941 tgcaataatg attttgattc ctttttttcg agcgttttgt gcatattcaa acatcaaatc
     3001 tggggttcga tgagcagaaa cgaccttttt ttcataagac acaccgaatt catccaatat
     3061 ttgacatgct tctttcattg tttcccaatc tgaagtactc cccataatca cagagatttt
     3121 tgctgtcatt gtttttcctc ccagtttttc tacattcata ttttaacaat cacttttttt
     3181 gatgtcaaag aaatttcgaa tgttaacaaa gaaaaacatt ttattgttcg tgtttattaa
     3241 agataaaaaa aaggaatgtg acaaaagtta ggtcacattc ctttttatgg ataaacggtg
     3301 ttctcctgcc aactcctcgg tcacaaggaa caatattcaa caaacatgag aagctttttt
     3361 ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct tccttcaacg
     3421 gtgttcaatc agctgaccct cggtattagc tcaaacaaac ggcaaaatac gaggaactat
     3481 ttttccgttt gtttctctaa caatcaggtc aaaacgctga tttcacaacc tttcttttgc
     3541 tctattactt cttgaaggct tcttccaata tttcttcatc ttttttcaat ccattaaaga
     3601 tcaaattcaa tatgatagcc gtaatgctac tcattacgat gccatttcca gtgaacattt
     3661 gcaatgtttg cggcatttga ctgaataatg tcggcgtaac attgaaaccc agcccaaatc
     3721 caacagaaac agcaacaatc aatagatttt tatcattgct gaaatcaact tccaacagca
     3781 tacggattcc ttggactgcc accatcccga acatgactaa cattcctcca ccaagtaccg
     3841 gctcagggat gatttgtgct aacgcaccga ttttagggaa taatcctaaa acaatcaaga
     3901 agaatgcaga gaaatagatg ggttttcttg ttttgattcc cgaaagttga accaatccga
     3961 cattttgaga aaagccagta taagggaatg tattgaaaat accgcctaag ataacggcaa
     4021 gtccttctgc acgatatccc tttttcaatt cttcttctcc aacatttttc ccagtaatgt
     4081 caccaagtgc aaaatataca cctgttgatt ctaccatgct gaccatggaa atgatgatca
     4141 ttagtaaaat cgaccatacg tcgaaagttg gtttaccaaa ataaaagggt tgtggaacat
     4201 ggaacaacgg cgcatctcca atcgcgctca gatcaatcat tcccatgaaa gcagctaaga
     4261 tgcttcctcc gactaaacca atcaataccg cgattgaacg aatgaaccct tttgccagca
     4321 tttgcacgcc tacaattagg agaatcgtca caaatgcaag tagcagattt gttttgtcac
     4381 caaaatcagc tgctgttgca ttacctccac ccattttctc gattgctaca ggaatcaaag
     4441 tcaaaccaat aacggtgata accgttcctg ttactatagg agggaatagt tttttaattt
     4501 tagaaaagac accagaaact aacacaacaa agatcccaga agcgataata gatccgtaga
     4561 tagctcctac tccttcattg gttccgatca agatcagtgg agctactgct tggatcgcac
     4621 atcctaaaac gacaggcaag ccaattccga aaaatcggtt aactgtcaat tgcaataatg
     4681 tcgctaatcc acacataaag atatcaattg aaatcaaata agtcatctgt tcttgattaa
     4741 agcctagtcc tgtaccgatc aaaagaggca ccgctactgc tcctgcatac atcgctagca
     4801 gatgctgcag ccctaaaacc gcagcttttc cattttgtgt ttctttttcc acgattatgc
     4861 gtcctcctca agaaactcaa cttgtccatt cgtcaaggaa gcaatacgcg caagagaaac
     4921 gactctcaat cccatttctt caagtaactg acgtccatct tggaaagact tttcgatgac
     4981 gatcccgatt ccttctactt gcgctccagc ttgctggcat aattcgatca atccttttgc
     5041 cgcttgtccg tttgccaaga aatcgtcgat aatcaatact ttgtcttctg aagataaaaa
     5101 tttacgagaa atagaaatcg tacttgtcac ttgtttcgta aaagaataaa cagaagctgt
     5161 caataattct tcatccatcg tcaaactttt tgcttttcta gcgaagatca ttggaacatc
     5221 taattctctt gcagcaaata aagctggtgc gatcccagaa gcttcaatgg tcacaacttt
     5281 cgtgattcct tgttctttaa atacttctgc aaaccgtttt cccatttgtt ccatcaatac
     5341 agggtctact tggtgagtga cgaagctgtc gacttttaat acgccctcgc ctaatatacg
     5401 tccgtctttt tgaatacgtt caactaattc tttcacttta tttgctcctt tatcatttat
     5461 taataccgaa ataaccgctt ttgtgcaaaa acctactatg tttctgttaa agaaaaacac
     5521 cctccacttt atcataagaa aagaagggtg aaatagccaa tcttttactt atagtccagc
     5581 atttacggta ctggctagaa actgtcatcc atattatgac gatatataag taggtatgta
     5641 aaactaataa tagtgtaaat tcattttctt gcaactgttt tgcacgtgaa ggcagtttgc
     5701 catatttgaa taaaaaatgt tgtttcaaat attgaaaaca gtctaacact actttctgaa
     5761 actgacaacg tggtttactt atttttttgt aaaatctaat gatttttaat gcattattcc
     5821 tttttgttcg ggtttaaata gttattttta ttttctccta tctaaaaaag cagcccacat
     5881 aaaaagaagg catgacaaaa agcatgtcat agcccctttt tccgaataaa tagtgttcaa
     5941 aagacagcct atcggcaata aggcaagatt ttataaacta cgggaagtaa tttccgtaaa
     6001 tctattctta ttcttttaag ctgacaagct cgtcaatcac tactgctttt cctgaagcaa
     6061 tagaatgatt tgctgcgatt cctgtcaaaa tcgacaaaat accatctacg tgagttgctg
     6121 cccgattcca cggatcttct tctttttctt caccaaatag atcatctaat aaacgcggat
     6181 ctcctccgcc atgtccgccc gcttcttgtt cgacgattac ctcatatggc tcaccgaaca
     6241 tcggatagac atggatggac tgaagaacgg ctgctccttc atccttttta tctccacctg
     6301 cattgatgta agacttttcg atcactttca tttcgatccg tcctttgctg ccattgaaag
     6361 aaacattgaa tccttcccac ggcatatacg cgtttagcga atacgtcaaa atcgcaccag
     6421 tttcatattt gaccgttacg cctagtgtat cttcaataga gatatcatcg gaaaatacac
     6481 tgcggtcacg aatatatcca ctgtcttttt ctgcttctaa atacatttct cgcaattcct
     6541 cattggctga caggtctata gcaaatggat cagctttagc tagttcgctt ccatagcatc
     6601 gactgtaaaa tatttctact ccgcgtttct ccgcattatg catcccataa aagtttaatc
     6661 caccgaatgc aaataccttt tggggacggc tgttcaacca aaaattcact agatcaaaat
     6721 gatgggtaga tttatgaaca agaagcccgc cgctattgtg cttatttcga tgccatcttc
     6781 tgaaataatc tgccccgtgt tgcgtattta acagccattc gaagtggata gaaaacacat
     6841 caccaatcac atcactttct atcagttcct ttatcttcgt gtgatacggt gcataacgat
     6901 aattaaaagt gactcgcagg cttcttcccg tctgtttttg gaccgttatt atttcttgag
     6961 ctttttcctc atcgatagtc agaggttttt cgcaaataac atcatatcct tttttcattg
     7021 cacgaatgat ataatcatga tgcgttcgat ctactgacgt aataatgact tcagccggcc
     7081 gtacgtcatc cagtagttgc tcaaattgtg atggcataca tgtctttact tttttctgtc
     7141 catacttgtt cacaagtatc cgatttgcat aatccatcct tgtttgagat acatcacaaa
     7201 aaccgactag ttcacaagtt tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg
     7261 ctctcccacc aagtccgact tgtacatatt ttttcttcat ttaaagtcat tctccttttc
     7321 ttttaagcaa aatactgtga caaatggcag ccctggatac agtaatgcag tgagtaaaaa
     7381 atcatgctgt atacaccaga acagtccata caaaaggcat aaataccaca gaaacccctt
     7441 gacgtttttt gacaacagcc agatattcct tttgatcaaa taaacaagac tatctgcttt
     7501 atcttctttg cagatatcaa ttagcgtact aaaaaaacta tgggatacaa aaagcaacag
     7561 acacaataga aaaggaatca gataccaagc tccaatcttt cccactaata aataccaatc
     7621 aactgcaaaa ataatgacaa acagctgaaa caacgtaatg atgaaacaca tccgtttgat
     7681 ttttcctttc ctccaacata cctttttttc tgccaagtaa tggacagata gataataaga
     7741 aggatacatc atcaaaaaat aaatcagata gacaacacga gcagacagca ctaatcctaa
     7801 agtgaacaaa catcctagaa ctaaagcgaa aagagcatta ctgataagac tggttaaaaa
     7861 aatccgacag acatagtagt tgagcttgta aagaaacatc tcttctattg gctttttcat
     7921 attcagccct tcagaccgct tgtactgatc ccctggacaa gatatttatt gaagaataag
     7981 aaaatcagaa aaaccggtag cagcgacaac gtagacatcg caaacatact cgcgtaagtc
     8041 gtactagatc cagcgtctgc aaataatttg atggcaatcg ataccgtgta agatctcggc
     8101 ttgtttaaat aaatcaatgg tcccatgaag tcgtcccatt tccaataaaa ctggatgatg
     8161 atcgtcgtga tgatcgaagg tttcagcaaa ggaaaaataa ttttggtaaa gacactatac
     8221 ttgctgcatc cgtcaataat cgctgattca tctaactctt tggggatgct gaccatgaat
     8281 tgcatgatct ggtaaatgaa aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca
     8341 tacgtcccaa ccaaattcag ccgattgtaa ataataaact gcggaatcaa aatcacttgt
     8401 gcaggaatca tcatcgttac taacatgacg acgaaccaga atctttttcc tttgaaatcg
     8461 attctcgata atgcgtaagc aatacaagtc gagctcagaa ctgtccctat cgtcgaaact
     8521 gcagagatga caaacgaatt cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc
     8581 catccttttg cgtaattttc ccattgaaaa gtctctggaa tcaaattcag cgaaccactc
     8641 ataatctctt gatccgtttt aaaagaacct gcgatcatcc ataagaccgg ataaatcatg
     8701 actagaccta atagcaaaat aaaaccgtat tgaaaccatt tcgttactgt attattcatt
     8761 tcttcctcct ctcattcaca caacgaccca tttttttgat gatttgaaaa tcagaaaggt
     8821 gacagctccc ataatcaaca gcatgatcca cgaaatagca ctagcatacc ccatagaatt
     8881 aaaagaaaaa gcttggttat acacataaag agcataaaag ttcgtggcat cattcggacc
     8941 accttgggta atgataaatg cctgcgtaaa agtcataaac gccgcgattg tctgcataac
     9001 caagttatac aaaatgatcg gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca
     9061 tttgttcgct ccatcgatct ctgccgcttc ataatacgag cctgggatct ctttgatacc
     9121 tgcagcaaag atgatcattg ccgaaccgaa ttgccaagca gacatcaaaa caagagggat
     9181 catgaccagt ttttgatcgc taaaccagga aagcgcaggt gcgcctaatc gttccaggaa
     9241 aatattcact aacccgtttt tagaaaacaa ctcccgccaa actagagcga tagaaatact
     9301 gccaccgatc aaactaggca cataatacaa agaacgaaag aaagtcgttc ctttgatatt
     9361 cctcgttaaa agataagcaa tcaatagacc aaagatcagt ttcacaggta cagatgcaaa
     9421 tacatagaga aaagtgactc ggatggagtt caaaaaacgg gcatcactca gcatctttat
     9481 ataattatcc aagccgatcc atgagactgg atttcccaaa cgataatcgg tgaacgaata
     9541 ataaagtgac aagatcatcg gtatcactgt tatcaataga aatcctagga tgaatggaga
     9601 agcaaatacg taaccgacca cattgtcttg atccaagaac cgttttagtt tttctttttt
     9661 catgccttcc tcctgctgct atcctagaat gtttgacgca aactcataaa gactctctgc
     9721 tgcttcttct gctgttactt cttcataaat gactttttca actaaaagat tgtattggtc
     9781 ttgaatttcc ggattcaaag cactatcaaa gacattcact tcgccgttgt ccatatcgga
     9841 aatcatttct aaataatcaa atgtcgcttg caaagttgca tcatctgcac gagcgagctg
     9901 ttctcgaata ttcgacatga ttggtacacc acgttctcct tttaaaattt cattggcttc
     9961 ttcactgttt tgaaagaaat caataaactt agcagcttct tctttatggg ctgagctttt
    10021 tgccatactc agcatttgcg aagaacgtaa agggatacca gattctccat ctgcttttat
    10081 gcgaggtgga ttaatcaact gaatctccat atcttcagga gcccctgaca atatttctgt
    10141 caactggtta cttgatagaa acaccattgc cgcttctcta tccctaaccg cctgaaaaga
    10201 cagatccgtc gtacttgcag ctccaccacc tggttctgga tgggcccctt tgtctaccag
    10261 atttttacgc agctggaaaa aatctgttag atagtctgga ttttcaaatc ccaagctgtt
    10321 agatagatct tcccgattat aaaaattcaa tcctttctca acttgtggta tttgaataat
    10381 acagccagct ataaaatctt cgaatcttga aatagcatag attcctaatt cttcagtgat
    10441 ttgatagctg atttgttcaa attcatccca tgtccaattt gaagctggtt cagggatatt
    10501 taattgttca aatatatttt tattgtaagc aatcgcaaac gaattcaccc cgttagacag
    10561 tccaactaat tgcccgtcgt aagtcgtcgt tgctaaatag gattcatctg cattagaaat
    10621 atcaattatc ccttgctcca catattcatc taatggttca atctgattga tgtattgagg
    10681 aaattgattg ttcagttgga aaatgtccca gatatcatct gatgctgcta gtgtacctaa
    10741 ttttgtgtaa tatccttcat aatcataaaa ttcataatcg atcgtcacat ggggattttt
    10801 ttcttcataa agctcgatta cttttactgt tgcatcgtgt cggacttgcg aaccccacca
    10861 agccatgttc agcgtgattt ttttgtcatc ttcttgccca caagccgtca agaagaataa
    10921 cataacactt aaagctaaga tgattctcat tcttttcttc attctctgca cctctacttc
    10981 cattgttatt tttcaatata ctgtattttt atacagaaca tatttattaa acgatctttt
    11041 tcaaggggaa taaaaaccaa gacttgcctt atcgttctgc ctaggatgaa caactcaata
    11101 ctttcaagaa aaattagata gtgctgattc ttctttcatc gctaaaaaca cttcttgctt
    11161 caatgatggg aggataatta cgcaaagaaa aaaggatata agacaactgc ccaatatcct
    11221 tgacatagct aaataagcat gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata
    11281 actagtaatt ctgtatgaaa ttaggtacaa tatacacgtc gatgcacaag cattgactat
    11341 ggcaacttga ttggtcagtt cccgctgact tttcaagccc cgacaattta gctgctacta
    11401 aattgtcggg tgcctttttt agtttttatt cgtttttcat tctatagaaa gcgcttcaat
    11461 aaatcaagac atttttttgt tagaatgcta ataataacaa aaggttttaa ctcttcttat
    11521 gtacattaca ggtaaaaaaa caaaaaaatt tctctttttt ttgaattctg aacaaatagt
    11581 actggagtac aaaagacgag gaagagccac tatctgctct tcctcgtctt ttgattctca
    11641 cctgttattc atgattcttt ttctcagttt cccgatattt ttccatttta cgactaaaaa
    11701 gctctccgct acttggtggc gtatatgtga tggcatttgc tcctgcttga atggtctcag
    11761 caatactttc ttccgttgga ccacctgtcg caatgatagg aagttcagga tattttttac
    11821 gaaaatggcg tactgcttct gcagtttctt tacctgcact gatattgata atcgaaatac
    11881 cagctgctaa cttttcatct atttctgtat atttcgacgt taccgtactg ataactggaa
    11941 tatcaactac tcgacaaact tcttttactg tctctaaagg cgtaggaccg ttcaaaacga
    12001 cccctaacga accttgcgct tctgcgaaca tgctcatgta ggaagaacga gcaccttgtg
    12061 tcaaaccacc acctacaccg gagaaaactg gaatgtcagc tgcctcaata atgcttttag
    12121 taatcgctgg atgaggtgta aaaggataca cagcaattac tgcattcgca tttgtattac
    12181 gtatgatcgc aatatccgta gtaaagatga tcgaacggat caattttcca aatatcttga
    12241 tcccgcttgc ttctaaaatg atttccggta ctcttacgat atcttttctc aattccgtca
    12301 tgatttctgg cgtaaattgt tctgccatca gccacttctc ctttgctatc tccggtcata
    12361 ctgattcaat tgatacgtct cgatgacctg gatgattttt tgcgcataac cggggtctgt
    12421 cgcataacct gcatcttgca atgcttgtgc ggcttcctga tagttggtgg ccgtaatcac
    12481 tttttcatac agcgcaggat tccaatctac tccttggacg aacagttgtg tatggtcatc
    12541 catggactgt tcccatgaat catacacttt gaagtctcct tggatagtta tccattcttc
    12601 gttaacaaat tcctttgttt ccaggcttac tttcttctga tccccataag ccttgatccc
    12661 gaaaagatta ttgtatttgc tagccaaggt actttttccc caatttgatt ctaatatagc
    12721 ttgtcctaaa atgatactag gaagcactcc ataaccatct tgtagttctt gggcatgcgg
    12781 aacaagccgt tcaataaatt gctgatggga ttgcgcttca ttttcttgct gattatcagt
    12841 aaaaggagaa gacagattct tcaaggaaaa aacaaaggct aagagtatca ctgctacacc
    12901 ggcaaccact gctggccaac ggatattctt gcgcttttgc tttttccact tttttttcgc
    12961 catgatcaat tcctactttt catttaattc attcagatat tcttctagcg tcaattggtg
    13021 tttggtgata tactcagcgt tttctttacc gacataacgt aagtgccaag gttcatatgt
    13081 gatcttagtt atatcttcgt gtccttttgg ataacgaacg ataaatccat actgcgcagc
    13141 attatctgcg atccatttag ttccaggctg atctccgtaa cttgcatcca atacagtaga
    13201 aggataactg ttgtaccagt tttgatcgac gacatcgact gccagacctg tgtggtgttc
    13261 gctgtatcct ggctccgtaa tcgtttcttt agtaatcttt gtcgcctcct cttctgatac
    13321 accttgacga ctcatcacat cttggacatt ttgttcgaaa acttgttgtt gggatgatac
    13381 agaccggtaa gctgagacca tcactaatgg aaatccagct gcttcagcag ccttggcaaa
    13441 tgcttcatat tctggagcaa tgcgtttgtc gatcaagtat ccgttactta atgatgccaa
    13501 ttgagtagac tcatcaattt ctttttctaa tttgtgatct ggaccaacca aaactagctt
    13561 ccaatcgtct gttttaacat ctggcaattg tttggacgta cgatcttttg tttctttgtc
    13621 ctttaccttg ctgctggttg tttgactgtt cttttcttta ctagcagttg ttttggtgct
    13681 tgttcgttcg actgcttgat tgccggcttg aacatgcgaa tcatcatcgc taaaaataac
    13741 atagcccatt gctgcgatca taatcacaac tgctccaaat cctaaaaatt tattcacttg
    13801 aaaaatcctt tcgttgttct tcacgttccc cattcgcctc atgaacgtaa gtttcttcca
    13861 gattttcatt cacccaatcg agaagttcga ttttttctcc ttcgatctgt ttttgaacat
    13921 ctgctggcag acgaaaattc aaaatatcat catagcccca ttcatcatat tgaatactta
    13981 ctttcaagtc aaggtatcta ccagcctgct gattattttt caaagggatc agctcgactt
    14041 ttgctgcttt gctgctaagc catttttgcg cgatgcgtgt tttgatttgc ttgtactctg
    14101 caacggcaac gtttcgtttt tcgggaaaaa taatcgtttg acgcaaaatc ttttgcgtta
    14161 gcatccattc ataatcagtg aaaagatttt ttactttctg catcgcttca gatggcagat
    14221 ttttctcgcc atctttccat ttttgccatt gttctggact gatcagcaag tattcatcta
    14281 aaaaattttt ttcagtttca aactgttcta ataatacact cataataaga tcaatatata
    14341 cttgatgcac gtttatcccc tccttttaac tattctactt aagttttgat caaaaagcac
    14401 ctcttaaatc gctgctttca ttttttttaa tgtttcaagt cctctcctct ttattcttcg
    14461 tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa ggagcgtttt
    14521 cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca tgagtttgcc
    14581 gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac ttcagaaaaa
    14641 gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta tcaagaaatg
    14701 ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca gcatatcgac
    14761 catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc catcacgatg
    14821 aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt gatcttagca
    14881 gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat aatggataca
    14941 ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa agaaccagac
    15001 cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga tatcggtacg
    15061 tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt tgcctctaca
    15121 atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg taacaaagaa
    15181 aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt cggtatcgtt
    15241 gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg tgcggagatc
    15301 ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga agcaatgaat
    15361 tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg ctctctcttc
    15421 ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa aaatatgtaa
    15481 ttttaaaagg ttgtgaaaaa gctgttctgc atcaattaat aagaacagcc aaacaaaaat
    15541 agctcctcct atttttcgtt tggctggact tattaccgca gaggaaagct tttgaacacc
    15601 gtttaagaaa gaggttgtga gcctaccgtt cagctccgag tcacaaggag caatttcaaa
    15661 aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg acaggagaac
    15721 accgtttaaa gaagaggttg tgaaatcagc tttttgacct gaatattagc tgaattcaca
    15781 acctctattt ttggattttt tgttgggaga attctgcctt gatttcaata aaacttttaa
    15841 aagactgcaa aaactggaac aattctgctc gatcctcgaa ttcagggcga ctagtcggca
    15901 gaggcatttg ccgatacaat acataaactt cttcaatacg tgttaaaatt tgtctgccat
    15961 catttgactc tgaaaatgtt tctgcagtat aaatcaataa accatagata tgttttccat
    16021 aataagctgg ttgctggatt ctctctagat tttccagcat gtctttaatc acattggcct
    16081 gtgctcttct catagagaag taagtttcgt agtatagcgg ctgtctgagc cagtagtttt
    16141 cttgatgttc tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa tgttcgcatt
    16201 tgatctgcac atcttcttta ttttctgata aaagaaattc tgccatttgt ctcaaaatat
    16261 ttcgaaattc tttttcgatc tgtatttggt tttccttcag ctgcttcgta cgatctggca
    16321 tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc cattaatccc gcttcattgc
    16381 cgatcagctg ccaagacatg ttttcctcca ctaaataatg agtgaccaaa accgaattta
    16441 ctactatccc ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg aataaaaaaa
    16501 gactgaagcc aaaaatagta tacccaacag caaaaaaact gaacagtgct gttgccgtag
    16561 ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc ttggtatttc
    16621 ctacacttaa taatgcaatg atccctgcag ctggccaata caacagcgat aagctacccg
    16681 ctgtgaccat cgaaaagaag acactgatca ctgttttgac tgttcgcatt cctatacgca
    16741 tttacttccc ccttttttat tttaattata cttgaataat gaaaaactag ctatcagaca
    16801 aaagtttagg tatgatagta aaaagaccaa aaaggagaat ctctatgcaa tttggccgtt
    16861 ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt atcttgtttt
    16921 ttcatttgac tcaacgagga atcccgatga ttgctgcatt atctgctgtt ttttctttgc
    16981 gtcaggactt gactacaacc gtttcttttg gaaagtctag gatcatcggc aatacattag
    17041 gtggtacatt agctatcatt tatttttttg tcaaaagcta ttttcacaat gattttttgg
    17101 tagaattgat cttgcttcct attctagtta ttattgtgat cgtcgtgtca gatggtatta
    17161 acaataattc cggtatcatt tccgcaatag ctacgttgtt gctgattgcg ttgagcatcc
    17221 ctcaaggcga atccgctttg tatgctatcc aacgtgtact agatactttt atagggacat
    17281 tcattgcaat cggtatcaat ttctttcttc gtcctcctga aacagaaaag aaggaagaac
    17341 tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca aatttacaag
    17401 agatagaagc taagatccaa aaacaaaaga aaaatagcga ggactaattt tcttttgcaa
    17461 taaaataaaa aaatggagag aagaacttta aaaagtcttc ctccattttt gattgaacgg
    17521 tgaagtgtac tactttattc ttccttcatc gagttttgtt ctgcctcttt tggcgcttca
    17581 tacatattta cttggctagt agtcccacgt gtcgacaaat tctctacgat ttccttcaag
    17641 tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt tgtggcataa
    17701 tttgtcactc ggttcgctcc actggtttct cctgaactgc ttcctgtatc gacaacagag
    17761 attttttcga tatttcctag cggctgtgct gcttcgcgca tcaattgcgg tagcatatcg
    17821 ataaccatac tcaagacagc tgcttcgcca tattccttga aggcattggc gatcttttgt
    17881 tttgcttcgg cttctgcttt tccttttgct agtgctgctt cggcttctgc ttgtccagct
    17941 aaacgtgtct tattggcttc cgcttctgcc agtgcttcca cacggaatcg ttctgcttcg
    18001 gcttcagtca cttctttgac tttttgagct tgtgcttctt gttctttagc gtaccgatcg
    18061 gcatctgctt ttttcttcac ttccgaatca tattgctttt cacgacgtgt gatttctttt
    18121 tcttccagtt cgatttgttt ttgacgttct acgattttaa tctgcatttc ctgttcgatt
    18181 acttgttgtt gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt tgctttagca
    18241 acatcttgtt cttgtttgta ggttgctaat ttcagttctt tttcttttaa tgattctgcg
    18301 atctctgttt gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg agcttttttg
    18361 atacgcgttt cttttagagc ttccgcttct gctatatctg catctctttt gacttgggcg
    18421 attcttggtt tccctaaaga gtcaagataa ccgtttttgt ctctgacctc tttgatagtg
    18481 aaagaaacga tgatcaaccc catttttgca aggtctacag aagcgacttc ttgtacgctt
    18541 tgactgaact tatcgcgatt ttgatagatt tcttctacag tcatagaacc aagaatcgag
    18601 cgcaagtgtc cttctaatac ttctcttgct tcattttcca gttcttctct tgtcttgcct
    18661 aaaaactgtt ctgctgctgt agcaatctct tctacagatg aaccgatttt gatgatcgaa
    18721 gtaccatcac acatcaccgg tacgccttgt tccgtataca cttcaggggt ggagacgtcc
    18781 aatttactcg ataatagact gatccgattc gaacgttgaa aaactggcaa gacgaatgcg
    18841 ccacctccac gcacaatttt gattttgtta ttgctttcat caatgtgcac atttttgctt
    18901 cctaaataac ttccactgat gatcaacgct tcatccggtt ttgctgtctg gtacttcgct
    18961 acaaagacga tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc gataaaaaca
    19021 actggtattt ccatttaaca cacgctcctt taagctttta tcatgctttc ataaggcatg
    19081 acataacaga ttctttcctt gatctcaatg atcaatactt tcgttccccg ctcagctgtc
    19141 tgttcttggg ggctgtaaaa acaagctgga cgactaatgg aaccggtaac acttttcaac
    19201 tgaatttctc ccatacctcg tacagggatc ggtgtgatca caacagctac ttgtccttcc
    19261 agagtttttt cagaagacga catcgttgat tcggcatgtt tcatcggcat caatatatag
    19321 aaattaagca aaaataccaa cactatcgat atgcctccac tagcaataaa taaaaataaa
    19381 gatgattgat ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa agttaaccaa
    19441 gggataataa gcatcggatc aagcgggcca tcaaaatcga atacatctcc aaaaataatc
    19501 aataggactg ccaatcctgc acaaatcagc aatgtatacc aataaatcgt ctcaagtgcg
    19561 attcccccta tcatatacac gcctccttcc atttctacgt ctatcatacc aatgtttgaa
    19621 aattctgtat agcttctttt accgactcag tggtgttttt tataggaagg ttgtgtttat
    19681 cgttgacaat ttgttaaatt tttgttttca tgttaaaatt ttcacaataa attctttcga
    19741 ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac acgatcatcc
    19801 ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg atcaggaatt
    19861 ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt gctaaaatcg
    19921 ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca catgcgattc
    19981 aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt gtcatggacc
    20041 ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct ttctcaagtc
    20101 atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa gaaggaatgt
    20161 tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt attggtgcag
    20221 tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga caaaaaatga
    20281 ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt tttcttgccc
    20341 aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa cagttaagtg
    20401 aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct tctgataaaa
    20461 ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag cagctattgg
    20521 atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta aagagacgat
    20581 tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag aaactatttc
    20641 tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa ctttacgaaa
    20701 tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat atataaactc
    20761 catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg gtttgattga
    20821 tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa attataaaaa
    20881 agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt ttttattagg
    20941 caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg attcttttat
    21001 ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag ggaacttact
    21061 tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg tccggattca
    21121 tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg acggtgtcgg
    21181 gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga agggaatcaa
    21241 tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg gatttttaga
    21301 attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa aaaaggaaga
    21361 tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca gaattgaatc
    21421 gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga aatgtagaag
    21481 aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat gtatacatgc
    21541 ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat gatatcccag
    21601 tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc cgctacggtg
    21661 ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg atcgaaaagt
    21721 atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa ttgatcgatc
    21781 aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat ttgcctaaag
    21841 gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa gatactattt
    21901 tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc aagaaatatt
    21961 tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg gaaatcggcc
    22021 ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca gattactgtt
    22081 gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca actgtttact
    22141 tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt aattgacggg
    22201 ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca cgaaagttct
    22261 cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct acatgcgggg
    22321 ttacaagaac tctagggtac atttctacta attgctggat agcagggtct ggaatcgtct
    22381 cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca tcggttgcaa
    22441 aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct tgaagctctc
    22501 cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat ccttcattga
    22561 tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata tcgcttttgg
    22621 ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct gtctgatgaa
    22681 tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc gtcaacccta
    22741 tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta aacatttgct
    22801 catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta tttctcaaaa
    22861 gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac gatggtacac
    22921 gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg actcccacag
    22981 tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg gtctcatctg
    23041 ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg atattgtcat
    23101 gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat ttttcaaaaa
    23161 aatctatctc gttcggacga acaccgtaac atgcgattct catactttct tcctccattt
    23221 cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat acaggtgtca
    23281 gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt gcctccctat
    23341 caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc atgataacga
    23401 ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa actgctagaa
    23461 tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac catgaagttt
    23521 tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt gctagccacg
    23581 ccaatggtga acttaaacca gctaaataat ccatcggctg aaatagccac ggtgcagtct
    23641 ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta actgacactt
    23701 gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg ataatgattg
    23761 gattgacgaa catcgctttg atatttttag cttccatctt caagccactc atttttatgt
    23821 atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa tacatcacgc
    23881 cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta gaaccaaaga
    23941 tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa aaaggttttg
    24001 aaactagaat aagaataata taaataacta atccccaaat caagacattg attccttgca
    24061 tcagtaattc tgtattgata tcttgcatga aagctcgaaa tgccagtgct ggcaaagcca
    24121 ctgttaagac gactttcgat agaatttttc caacagattc agaaaaaatc cccttttttc
    24181 tacaaacaaa ccctaataat ataataaaaa ctgttgaagt gattgcactg acaatcgaca
    24241 tatctgtcaa agtcgatcga aaaatgtcaa ataagttcat ctttctcctc ctaattatgt
    24301 tcatttattc acaataaaaa aagcttttat tttgctaata cgtattaaca tttttaatt
//
LOCUS       DAXWLG010000025        24259 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXWLG010000025 DAXWLG010000000
VERSION     DAXWLG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.025"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..1435)
                     /locus_tag="ACGE4T_000784"
     CDS             complement(<1..1435)
                     /locus_tag="ACGE4T_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386087.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDK"
     gene            1557..2141
                     /locus_tag="ACGE4T_000785"
     CDS             1557..2141
                     /locus_tag="ACGE4T_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386088.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2403..3125
                     /locus_tag="ACGE4T_000786"
     CDS             2403..3125
                     /locus_tag="ACGE4T_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386089.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3256..3999
                     /locus_tag="ACGE4T_000787"
     CDS             3256..3999
                     /locus_tag="ACGE4T_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386090.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4005..4370
                     /locus_tag="ACGE4T_000788"
     CDS             4005..4370
                     /locus_tag="ACGE4T_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386091.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4386..4916
                     /locus_tag="ACGE4T_000789"
     CDS             4386..4916
                     /locus_tag="ACGE4T_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386092.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5132..5386
                     /locus_tag="ACGE4T_000790"
     CDS             5132..5386
                     /locus_tag="ACGE4T_000790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386093.1"
                     /translation="MMIGMRIQREQSYFEVSANNVIERCHYGQHFWTGEVRENIDREY
                     VQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV"
     gene            5493..5801
                     /locus_tag="ACGE4T_000791"
     CDS             5493..5801
                     /locus_tag="ACGE4T_000791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386094.1"
                     /translation="MYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMS
                     RFLKEREKKELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ
                     "
     gene            5938..6738
                     /locus_tag="ACGE4T_000792"
     CDS             5938..6738
                     /locus_tag="ACGE4T_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386095.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6775..6957
                     /locus_tag="ACGE4T_000793"
     CDS             6775..6957
                     /locus_tag="ACGE4T_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386096.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7068..7385
                     /locus_tag="ACGE4T_000794"
     CDS             7068..7385
                     /locus_tag="ACGE4T_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386097.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7388..7531
                     /locus_tag="ACGE4T_000795"
     CDS             7388..7531
                     /locus_tag="ACGE4T_000795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386098.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(7735..8214)
                     /locus_tag="ACGE4T_000796"
     CDS             complement(7735..8214)
                     /locus_tag="ACGE4T_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386099.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8298..9671
                     /locus_tag="ACGE4T_000797"
     CDS             8298..9671
                     /locus_tag="ACGE4T_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFF8386100.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            9694..10221
                     /locus_tag="ACGE4T_000798"
     CDS             9694..10221
                     /locus_tag="ACGE4T_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386101.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10208..10771
                     /locus_tag="ACGE4T_000799"
     CDS             10208..10771
                     /locus_tag="ACGE4T_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386102.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11193..11930
                     /locus_tag="ACGE4T_000800"
     CDS             11193..11930
                     /locus_tag="ACGE4T_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386103.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(11947..12363)
                     /locus_tag="ACGE4T_000801"
     CDS             complement(11947..12363)
                     /locus_tag="ACGE4T_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386104.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKYKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            12543..12758
                     /locus_tag="ACGE4T_000802"
     CDS             12543..12758
                     /locus_tag="ACGE4T_000802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386105.1"
                     /translation="MNLDKYNLYTAREASLILGKGRNYVTNIINYYPNRLPKGSFKKV
                     GDKTYIITEEGIEALRSQTENKNKKKE"
     gene            12755..14002
                     /locus_tag="ACGE4T_000803"
     CDS             12755..14002
                     /locus_tag="ACGE4T_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8386106.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            14155..14280
                     /locus_tag="ACGE4T_000804"
     CDS             14155..14280
                     /locus_tag="ACGE4T_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386107.1"
                     /translation="MINRCGDFNLGMGAKVITTPTYRGSFSSPNWYKEGVTRDEA"
     gene            14270..16660
                     /locus_tag="ACGE4T_000805"
     CDS             14270..16660
                     /locus_tag="ACGE4T_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8386108.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            16709..18544
                     /locus_tag="ACGE4T_000806"
     CDS             16709..18544
                     /locus_tag="ACGE4T_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8386109.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            18619..19476
                     /locus_tag="ACGE4T_000807"
     CDS             18619..19476
                     /locus_tag="ACGE4T_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8386110.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            19466..20575
                     /locus_tag="ACGE4T_000808"
     CDS             19466..20575
                     /locus_tag="ACGE4T_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8386111.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            20914..21714
                     /locus_tag="ACGE4T_000809"
     CDS             20914..21714
                     /locus_tag="ACGE4T_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386112.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            21820..22608
                     /locus_tag="ACGE4T_000810"
     CDS             21820..22608
                     /locus_tag="ACGE4T_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386113.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(22625..24070)
                     /locus_tag="ACGE4T_000811"
     CDS             complement(22625..24070)
                     /locus_tag="ACGE4T_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFF8386114.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
BASE COUNT         8919 a         3410 c         4701 g         7229 t
ORIGIN      
        1 ctttatcctt taattgaaaa ccctgatcca tagtcactgc atttggatat agaagcccac
       61 tataatgttt acgtaaattc acttggaatt tctgattata aaagtctaac agattagaaa
      121 taaaaggcgg aaaattactg tttttgtaaa attgattatt tccatttagg ttgtatagta
      181 atttgttaaa ttgctcctga tttaatttat tgtaaatcca tggtaatact tccatatcct
      241 tatctgctaa cctgaatgaa ctagcatcgg taagatgttc gatcataaaa tctatgaaca
      301 tctctttttc accaaaatat aaatattgat tatatattgt ttctaatgtt gaagatggta
      361 gcgaagaaac tctcattttt tgctggagta taggatcaac tagactattt aagtgatttt
      421 gtaattcttc attgttgttt cctagaactg caagggaacg taaacggtag taatattcta
      481 tttctgttag tacttgttgt ttcggtttaa aatcatttat tgatatttgt aaattttgaa
      541 ttgtctcgtc tgaaaaatca tgttgtaatt ctggatgggt agctataaaa gtaagtaaaa
      601 agaacccttt tgtatacgtg atctgttgat tttcaccaag tgatttatta atttcctctc
      661 ttgattcagc tgtgaaccta tactttgcaa tttctttttt tagaatattt aaatagaatt
      721 ctttattatc aaaaattagg gcattaagta gtctgaaagt actttcccta tctctatctg
      781 cttcctcatt tccataagat aattgaaaaa caaacttaaa ggctgtctta cataggtttt
      841 ctttggcttt ttcgcttagt ttatttagat agctattttt tagtgcagag tgaaattctt
      901 caggtgtaac agacatagca tgtatgcgac ttgcatattg tataatttcg atcgttactt
      961 tttttcctaa tattggattt tttaggaata taatttcata ggacttccgt ataacatcct
     1021 gacaagtttc ttttgttata tctcttaatt cccaagtatt accgttgtaa gtgactacag
     1081 gatgagctga ataatttctt tgatccttaa tatattttaa atcctcatat tcatagctat
     1141 caattagctc agttgaacta taagcttttt ctagtagttc catttcccat tttggagagt
     1201 ttggatcact ttttcttagt ctagctatat cttctaaaat tttcttagaa gtattatcct
     1261 cgtaaatatt tgctaaatca gataatttat caattaaatc taaaataagc gcactgtaat
     1321 tgacagctat tgcagaacga taatttccct gataaaaact acttctaact tcttctaata
     1381 atggaataat tctaggattt ttaacttttt ttatttcatc atctaatata aacatataaa
     1441 tctccttttt ttatttgtat gacaagttta ccataaaaaa aaattaaaaa gcgagacata
     1501 tttatgttgt tgtgttatca taataacata aatatgtaga gtgagatgat gaaaaaatgg
     1561 atttgaaaca atcgttttac atctggttat atcctgataa tcctatgtca ataatggctg
     1621 atatttttgt agttagcatc ttattgtatt tattttatct aattttcttt cacaatttaa
     1681 tcgggtacat gattcttgat tggaaaggaa tgttcgatat agaaagaaaa ccatttgcta
     1741 acaatcaaac ctataccgtc ttcgaaagtg gagattggga agaaaatgaa gttgatgtga
     1801 tccatgaaat tggattagct ttatcaggaa tacaagatgc tgatgttttg gtatcaatga
     1861 caacggatac tggatacaac gcttatgagg tattttatct tatgtatttt atatggcgtg
     1921 atggtcaaga aattatcaat ggtcgtgctg aaaaaacaat caattctaat actgaaattt
     1981 ctttttgtat gggtgaaggg gaaatgattg tatataaaga tgggtcagca aacattaaaa
     2041 cagcctttgg tgatattgaa acaagtatta aggaacttag ggaatatgtg cagaacccat
     2101 ataattttca atatttagca ggaagaataa atttagttta ggagtaaaaa tatgaaaaaa
     2161 attataaatc aaccaacaaa tcacttgata attgaaggtg ttattttcgt aacaatgtat
     2221 tttactgtag gaaatggcat ctcaaataca atcattcaaa aaaatccaca atacctatta
     2281 tttagtcttt atgcttggat tgtacaaatg gtttgggttt ccatattgat gctgttgttt
     2341 tattggatag tacgggttat tcttttattg ttaaacaaaa gaaaaaagga taggtgaata
     2401 aaatgaaatc aactaaattg aaggttgtat tgttatcgac agcagcatta ataaccctta
     2461 cagcatgtgg tacaactaat agtgctaata ataacaaaga cacaaagtct agtagtacga
     2521 tggttactaa aaacagcact acatcggcat caacaacaat gtcttcaatg actgaaaata
     2581 atgctgaaaa gtatcaaaaa tacgaagaca ttaataaagg tcaaaagccg gatgaagttt
     2641 ataacaactt tattaataca gtaaaaaatg atgaaaatta tgggaatgat ccattgcaaa
     2701 ataaaaatct gactatcgtt ctttacaaac ctgattgtcc agattgtcaa aaagcagaat
     2761 caaaaatcat gcaagaaata aaagaaggat ttagttatgc tccaacatta atgcatgata
     2821 ttaccataaa taaaccagct tacgagtatt acgcagttgc tgtagatatt actgatggtg
     2881 tacctgagtg ggtacaaaac attcctggtc tggctgatga gaacaatcaa tatcatacgc
     2941 caacagttgc agtagtaaca ccaaagaaag ttaacggcac actatactgg gatgtaatgg
     3001 gaatgacaac aaatacaact gatgaaatga ttgaaagtac aactaaatat tatatggatt
     3061 tgggagcata taaattatat caacaatcag agcctgttat ggacgatcaa tccaaagtta
     3121 aatagatctt tagggcgtaa gccctatttt tttaaaatta agtgataaca aataaatgat
     3181 ttctgaaaca aacgttctta ctcaactact cgaaacacac aagctaatta actagattat
     3241 aaaggagaat gcgtaatgaa agaaatcagt gcaaatgaag ctcatattcc gcaaaagaat
     3301 caggttggta tatataaggt gcttcatggt gaaggaattt ataaaataca gttacttacc
     3361 ggttacaata atggtgcaaa tgggaagtgg ttaaaggcaa caagtattcg tgaacttaga
     3421 agtgaagtag aaaaaattag gcttggtctc acaagtgaag aaatagctaa actgaaggga
     3481 aataattctt ttgtaattga aaaagaaaag gaaaaacatc tactttatcg tgagctgaaa
     3541 attgaagaat actatgaaat tgatttacct gaaaaattaa gaattattga tcaacgttca
     3601 gaagaaaaat ggaaaactga aatatatcca agcttaaatg actacaagac atcaggaaag
     3661 cccatgtata actacacagg ctttttaacc tacacacaat acaaatatgc ctttcgcaaa
     3721 ttaaaaggca ttaaacaagc tgaaattgcc ctgtatgaga tgaaaaaagt ggatagattg
     3781 aaattgattc aagatttagt gcaaaataca tggaatcaag cgattgtcga gctagaaaaa
     3841 gaaaatggga aaattttaac taataaatat ctgatcgtaa atgaaattga agaattaatt
     3901 ggtttatttt catgtgaaaa aggtgaagtc ttcttgaagt catcaccaac ctattcaatt
     3961 ttggacagtc cgtatcagtt tatacaggca aggagatgag ttagatgtct agcttgaaag
     4021 atgactatat ccaagcaaaa caagagtatg aaaaaataca gatgttgaaa tctgagaaag
     4081 tcgaattatt tttaacacaa aaattagcag aggatcatat tgctaactta gatgctaaaa
     4141 ctttagagga atatgatcgg cagtttaatc agatacatca caatatcatt ttgcttgttg
     4201 agcaggcaaa gcgaaggttt gaaaaagcgg aatatgatct attagatcaa ggaattgtat
     4261 gcttggataa gggattgcga agatattatg gtgcagagtt aaaaaagcaa aatgtagata
     4321 gaaatgcact aaaacatgaa atcttagaat tgataatgca acgaatttga agggatagaa
     4381 agcaaatgtc acaaaacatt agtgctgtaa aacaattaat agaagaagat ttagagacca
     4441 cagttcagag attagcggaa cgaatccatt ttgcaaatta tcaaaccatc tctaactggt
     4501 caagcaggaa cagaaatccg gaagaaattc caatgaaatt tttttatcga atcggaaaag
     4561 aatatggtgt tgatccgata aaaattttcg agaagaccat gatttattac tatcatggaa
     4621 cacctaacga agtgttagaa catcttttgg aagaggcaaa agaaaaatca tcaatattag
     4681 agctatcgca aaatgacaaa gttattcaag tgaaatgcgt tgatccaaca ggaaagatta
     4741 ttgtcggttt tgatattgaa agaaaatcca aaatagattt tattacacaa ttaaaaaaga
     4801 taaataattt agatattcta acgcttagac cagatcatga acagttgttt atgagtttta
     4861 aaaaacaatt gaaagaaata caaaaaagat ataacaaggt gtttcctaat gcgtgaaaag
     4921 ctcaaagaaa tcagagtttt ttggataatt aacaaagaat gtatcatctt atatactctt
     4981 ggatttctta gcatgatctt gttttggtgg ttatatttaa aacagtaatt ggagagggtc
     5041 aagaggattt ttatcaagat aaggatgtgg aaactgcatg tggaataata gattaaaaac
     5101 aggattgtta ttaatagtta tttcctgcgc tatgatgatt ggtatgagga tacaaaggga
     5161 acaatcctat tttgaagttt ctgctaacaa tgtaatagaa agatgccatt atggtcaaca
     5221 tttttggact ggggaagtca gagaaaatat agatcgtgaa tatgttcaaa gaattgtttg
     5281 ggatgcatat tctatcaaag attatcctaa aagtttaacg agtagattat tttacagtga
     5341 aaaagataat caaaaactct cagatttgat gatgaaaaaa gtatgactat tattacgaaa
     5401 gagaaacaga gtgctatatt cgaggggaaa aagcctacaa caaaagtgac tattggggtg
     5461 acaaggcgaa gcgattaact gcaaatttta gagtgtatga atcagagttt ataaacttga
     5521 cctttcttaa tagtgttttt attgattatg taattactac gaaacgcatt ggaagttatt
     5581 caagtaccta cgcatcctta gttccaatgt taaactcgat gagccgtttc ttaaaagaaa
     5641 gagaaaagaa agagctacag tatatcaaag aaatcatgcc ggattttgaa atgcacgaat
     5701 ggcaaattgc tttatcagat tggaaattgg cgaataaggt tcgtagaata acaccttttc
     5761 aagccaagcg ctttttgaga gctatgaaat ttgtgcaata aaaaaagcca ccagacaagt
     5821 tgatggagac taacacaatt accaacagtt gtaattgtgt tagcttacaa attaattatg
     5881 gtgtttagtc aatcccaaat tgtgaaatgg caccaataat tggaaaggaa aaatgaaatg
     5941 tctgaagatt tacgagagct gaagttacga aagaagcagt atgatcagta tttttccggt
     6001 ttgttgaata aacatattga agaaggactt gatcatgatt ttggaaaaga tgaatattgt
     6061 ttatattgcg aacgttacaa tgaaaaaatg tctgaatatc tacagctgaa aaaagagata
     6121 cagaaattag agaaaaaagt acatgcttgg aagtataaag attccatgtc tggttatgtt
     6181 gaatctcaaa ataagccaag aaaaaagaag caagcgtatg ttctaaagat ggtaaatggg
     6241 gaagagttaa ttgtattagc gtacagtgaa gaagaggttc gagaactcgt tgaaacacct
     6301 gtcaggaaaa aggttgaaac aataaatgag atagacgaca gccaatacaa tagcatctat
     6361 attgaaacaa gtcatggtgg atataagaaa ttagcaactt tccttagaag ttctaaagtt
     6421 gggataattt ctaaaaaaga cataagtgct gttaccgata gtttttgtac tttatctgtt
     6481 cctaaagctt atttccaata cgatagtaaa agatttgaac gcttagagaa attctgtaga
     6541 ccgtatactg tattgaaaac taaaaaattt aataaaacta aaagagtttc tatacagtta
     6601 ccgactggtc aaatctcatt aggtgaaaaa gactatctga taaaagtagg aaaagagtta
     6661 attataaaag taccggaaca cttatttaaa gaaaattttg agatacgtga aaccttggta
     6721 tggaaagaag aaaaatagtt tggaagacat agaatggtat aaggggaaat tgaaatggca
     6781 gtaaataaaa tattgcaggt actaatcata gacgaatttg gcggaggaga atggtttgat
     6841 gtgcagtcca tagactttga agaagaagtc gtagttatag aaaatattta cggcgaagta
     6901 tattcatatt cgcttgaagg tgagaatatt gaggatttac ggttgattgg tgagtagaaa
     6961 aaaacagaaa tatcaaaatg gagataaaaa ttaatagcat gtaaagatag tcatgcttgg
     7021 gttaaaaatg ttaaaattca aaaaaatgag tcagaaggga taaaagtatg aaaagagagg
     7081 aatacaaaca aagattaaac gaactgcttg aagaggacga aacattaact catggttcgc
     7141 ctgatgaaat tctatacatg attgataata tggtgatctt tggtggatat gaacttggta
     7201 ataggtcagt tgatcataat attttagaat ttgatgatgt atcatgggaa gaaattcttg
     7261 attggggaat tttggcggta cctgaaacta aaacctatat tagtgacact atggttcctt
     7321 tttttgagga attggattat aaaagacttc ccaaaaatga aaatcatata ctgggaggaa
     7381 actaacaatg ttaagtgact tatttatttt actgatctat ctaggtgtaa gtgaaattat
     7441 tgcgtatttt ttgtttgtca ttgctaatta tggtatttcc agtgcaatcg ttcaaatgtt
     7501 tagattacca gtaagtgaac taaaaaaatg atgagattat cagcatatct tttaatgttt
     7561 ttgaatgtgg tcacgttaat ctttgtagta atcggtgtga ctttcgattt aatggaatag
     7621 tgggaaatct ataatggaaa aaagagacac gaatgaacat attttttgaa tcaatattgt
     7681 tgatggtttt tgcgatcagc gcttaccaga aatctaaaaa agagtaatga ttgtttaatc
     7741 atcactcttt ttttgtaggg aaattgaaat atgtggagct tgtattggtt tagttaggat
     7801 tgcttggcta taaggagcta gttctttatc aaaaccatca aagtaggttt tcagtagcga
     7861 tttaactgat cgctcatctt gtttgatttg gataatttca gcatccaaat cggttgtgag
     7921 atcaggagct tttgatgcga ttacctgatt ttttttatct tccaactctc ttaggctgtt
     7981 taacaagttg taaaaagaat cattttgatt gttcattcca acgtgagttg caattttaac
     8041 agaagtttcg tccgtattgt catcaaaaga actcattgga atatcaagta gtaacgagga
     8101 tgtttctttt actgactttt tctttacagc attttttgtt tttccatctt ttttgctctt
     8161 tactcgagat gtttttggag ctagtttagg taaatcactt gttttatcag tcatttatta
     8221 gcttttcctt tctattaatt tatgttatta tattatcata ataacataaa aaagtacact
     8281 ttttgggggc gtagttattg aaaaaatcta agagaaagat tgccacattg cttatgttaa
     8341 tggtgcaatt tacagggatt gcaatgagtg taatcacaat aaatccgcaa gtgatacatg
     8401 ctgatgggat agctacttat gatgcacaat ttaactgtac tactgaaggt agtgggtcag
     8461 gaacagatag cgatggaaac gatactggaa gtggcgctgt aacaggtgat gtagccaaaa
     8521 atattgaaga tatttacaaa tacatgaatg gagaatatgg cttatcaggt gctagtattg
     8581 caggtatttt aggtaactgg aaacaagaat caggtattga tcctcgagca gtagaagggt
     8641 attacggaga tggacagaga gaagttgact tcatcaaaca atttactgat ggtctgatgg
     8701 gtattggcta tggtcaatgg acagctgggc gacatacagc attagttgat tggtctaaga
     8761 aaaataatgg cggttcttgg tggactacag aagcccaatt ggactttatg ttcaaaggtg
     8821 atgggggatt cgtaggaata ttaaaagatt atgctttaaa tgcttctgat gatgtcgtag
     8881 aaaatgcagt aacttttcat aaaaactggg aaatttcagc tgattccgaa tcagctgtaa
     8941 tagctgtacg tggtgaaaat gcaaaagaaa tctggaagta catgaaaagc aagggtatgg
     9001 acggtgcaaa agatgaaagt aagatcaata aaatatccgg aggttcaagt accggagatg
     9061 gatcgaataa ttcatcaagt tcagcatcag gaacagtaga agatgcctgt gcaactacgg
     9121 atgacgctaa ttctggttct ggatcaattg ggaaagaagg aaaaattaac ggtaaaaaag
     9181 gtcaagtaat agcgaatatg acttatgaag aagctttgaa aaaatatggg aaagcgatca
     9241 cgctaccaaa attcaaagaa ccagattggt caacttcacc ttttaacgga atatatcaag
     9301 gtcagtgtac agagttgacg tgggcttata tgagccaatt atacgatggg aatcaaccga
     9361 ctactggaaa tggtggttat gtatgggaaa gctacaaatc ggcaggagca aaaataacga
     9421 acaaaccaat cgtaggttat gggtttagtg cttcggatgg atggttatgg gctgttggag
     9481 atgcagggca tactggtgta gttatagcgg tgtttgaaga tggatcattt ctatgtgcta
     9541 actttaatgt tccaccaacc caagctccaa caaggggagt ttcggtaacg ttgattgatg
     9601 gagtggacgg atcagataat gtgcatttct ttagcggtgt aggaaaagct aaatttgaaa
     9661 tcgaaaaata aattggaatg aaggacgata tagatgaata gtaaagtgaa agatacaatt
     9721 atcatttctt tgctttctgg tatgttatta gcactgcttg ttggaatcct attcttcaat
     9781 aagcagaatc aggacataga agctgaaaaa cacagtgtgc atattgaaga taagctgaca
     9841 ttctttaaag aggttaaacc aaatgaggtg gatggattac ggaaagattt agaaaatgat
     9901 attcaagaaa tgttgaatgg atcagttcaa aataagtttg cagatcaatc aggtgaagaa
     9961 tttttgagaa aactatttat tgtaacaggt aaaccaccaa ttactgataa gagtagtgag
    10021 aaagaaaaaa tcgagtacta taagcctttt acttttaagc taacaaactt tggaattaag
    10081 acatctagta gtgggttgta tgaagcattg accgattttg aagttcaata caatggaaaa
    10141 aaagtgtatg ataccacatt aaccatcaca ctaaataatg ataagaaagt gactgggggt
    10201 gctttttatg caaaatcatg aaatcactac tgctaaaaag gcgatcggaa tactttccat
    10261 aattgtgatc ctttgttgtg tggtactatt tcaaaagaaa aatgcttatg ataataatgt
    10321 caaagaattg gaagacctta aaagtaaaca aatcgaagta gtcaagaata accagaaagt
    10381 gaaagctatg caggaagctc aatacaaaga attgggaaat gctgaaatta aggaacaaag
    10441 taggaaattc tttaatttat tttacaactg gaattcatgg gaaaaatata cgaagaatat
    10501 gaaggaaata caaaaagaat ttcctcagat agtaaagtct agtcctgttg atatttctgg
    10561 cacgatggta ggcactggta atagtccaat atctagctat actgctgatt actatacaac
    10621 aaatcaaaaa ggtaaaatgg ttgaattgat tacacagaca agaaatactc caacaacagc
    10681 aacaacaaca atttggcgtg gagtaacaag cataggagaa aatcaattgt tttatttgga
    10741 ggatttagat agctatgtaa gagcagagta aaataaaaaa agagctaacg gtaccaacgt
    10801 tagctctttg tgctgacgat gataacaaaa cctaccgaaa aaggattgtt tcatcacaaa
    10861 cacaaacaga aaagaattga taaaaaaccc gaacctcaga atttttatta atcaattcat
    10921 ttagattata ataaattgag ctgaacatta caatacgtat atgttgtaaa acgtaaaaaa
    10981 agtcaaaagt aaatactttc ttaaaaagtc aaataaaact attaaagaca atatatatgg
    11041 tttatttgac ataagaaaat ggtctagatg ataattgtaa tatagaattg tagacttaaa
    11101 taaagcataa aatacctttt atgataaggt atagctctta attttaagca gttttagata
    11161 taagattgaa agtaaagtgg gggagaataa taatggcaga tcataaatta gaaaagttat
    11221 tagaagaaac gtggaatcca aaagaattta gtgaattttt tatggagaat tttgaaactg
    11281 acttagcagt gattgttaaa gatgctctta gagagcaagg gtatcctgaa actgcaaact
    11341 atataaacat taattttact ttgtatactg aaaataaagg aacttgggat ttttgggcta
    11401 ctttagcaaa taaggagtta agtgataaat cagacacagg aattcgtaac ttttttgaat
    11461 caaacagaga tgattatatg tatgctaaca atcaaaataa attgaacttt agagtagagt
    11521 ttgatgaaac acctgaagaa tttatagaaa gacaaccgcc taaagaaaat gtagcaaagg
    11581 tattggaaga tcgttggaat tcagatgaaa tagtttcaac aattagcgaa ctaggcggac
    11641 aatatgaacc gctagtagaa gcggttagag aagagctaag actgaataag tttcctgatg
    11701 ttcaaaatat tgatgtttct caaattgaaa taaacgtcaa gatcacgaat aaacttgatt
    11761 atggtagttg ggcagatata gcattagaaa aatacatcta ctcaacgtta aaagagttta
    11821 tcgaaaatag aatggatatc atgtatctac agcatccaca gtatttaaac tttggtgttg
    11881 aaatagctac acctcttgaa gaatggaaaa tggagcaagg gcttgactag cctttgctcc
    11941 atttttttat tttacaaatg aatacagttc gtcgttttta ccaagaatga ttgtttgccc
    12001 aactttgaga ttgtcaggtg atccttcagc ggtttgtaag gagaacgttc cattgaactt
    12061 atcggatacc gttataggta gctttaattc tgatccttcc tcatctttga gaattgctgt
    12121 ttttcgttca tcaaaataaa cagtctttat taaataattt ttcgtagtga tctcagcaga
    12181 tttgtatttt tttgtagtag taccagtttc tttagaatta gaggtatttt ctacgactat
    12241 tccatctttg gcatttgagc ttgtgtttat ttgattgaat attttcagcg acaagataac
    12301 gctaagaata gtcaatatag aaacaagtgt aattaatact gataatttcc tttttttgtt
    12361 cattttttct taacatcctt ttttgttttg attagttaga tattccttat ctaacaagcc
    12421 tttttaggct attaaatgaa gataaacccc aaaaaatatt gcaattatga cagtgcaatg
    12481 gtactattat tatacaataa attttgcgaa aggggtaggt ctaaatcgca aacaaaaaga
    12541 aattgaacct agacaaatac aacttgtata ccgcaaggga agcaagtttg attttgggta
    12601 aaggtagaaa ttatgttaca aacataatca actattatcc aaatcgtttg cctaaaggtt
    12661 cgttcaagaa agtgggtgac aaaacctata ttataacgga agaaggaata gaagcattgc
    12721 gatcacaaac agaaaataaa aacaaaaaga aggaatgaat atgaagagaa attcgcttgg
    12781 tatggtttta ttggcatcag ctgtacttgc ggggttatca gtaccagtaa cctcacatgc
    12841 tgatgaatgg actgcaaaca caccggatca aatccaaatt acaacaggac aatcaagtta
    12901 tgcgttcaaa aaaggagata ctatttggtc aactgtagag aagcttaaaa gcttaggtta
    12961 caagactact gttgctgagt ttgccgaatt gaacaatgtt gatttggcaa aaggagaaca
    13021 acggactatc ccgattggac gaactttcaa catcagtaaa gatacattaa cagttaagga
    13081 agcagatggc gaagtagtta gtcaaactgt tcaaaaagat aaacctgatc ctattgaaaa
    13141 tacggagcaa gagaacaaaa aatccgagac tacaaaagaa tcggaaccaa cagcaaacac
    13201 ttctgataag acagaaacaa gtgcttcaag tgttgatgga gcaagttcat ctaatacaaa
    13261 taattctaca agtgagaaag aagcatcaca gggaacaagt gaaagtagca ctacggaagc
    13321 tgatactgga acatcaacaa ataactcaca atcaacatca aatgatgcaa atgaatcaac
    13381 cagtacatct tctagtgatc aatcacaaac aggttcgagt gatgaaactg acagttctac
    13441 ttcaccaagt aaacccgaac aaccaactaa tgttgtgcta ggcgttccat ttattagtca
    13501 aggtaacaca atgctttgcg aaggaacatc tttgctagaa gcgttacatt ataaaggtgt
    13561 tgcaacaaat caagatttaa tgacttttgt taaatcaatg ccactatctc ctgacaataa
    13621 tccaaataat ggtttttctg gtgaatggag acacaatgtg gatggaactt atcaaggtat
    13681 gaatccggct cctgtagttt catggggtgt tgctaatggc ggaaatgttg tagatatttc
    13741 tggttcatcg gtacaaggat taaaagatga gattgtaaat ggaaatcctg tagtagcgtg
    13801 ggtaacttat gcctttgaac ctgctcaaca taaaccaatg ttttggggcg atgctatttg
    13861 gaatcgacat gtagtagccg ttgatggatt taaagacggt ttttaccatg ttgtcgatcc
    13921 tgtgtttggg gcaagttgga ttaatgcagg atcatttgaa acagcatata acgtatctca
    13981 attagcggtt tcggtacgtt aataaaagat atcgtttata tactaagtag ttatatagtc
    14041 tttatttgtc cttgcttaac tacttagaaa acttgttgct catggtgggt agaactaaaa
    14101 ggggtgatgc tatgaatact agagcgttga aacagcttgc tttcaacgaa aaaaatgatt
    14161 aataggtgtg gtgattttaa cttaggaatg ggcgcgaagg tcattaccac accgacatat
    14221 cgtggctcat tttcttcgcc taactggtat aaagaaggag taacgagaga tgaagcatga
    14281 aaaaacgata attttaaaat caaaaataat ttattgtttt gcgactttag taatgatgtt
    14341 atctacgctt ataatgcctt tttcagcaat aagagctgaa gcagatgtca caaaaccgac
    14401 aagtgcaaaa tttatagaaa ctggcgtaaa aacagacggt tatattaggg ttaatatgcc
    14461 gaatcatcca aatgaatgga tgatttcaag tcaatttaaa gattctcatg gaaatattgg
    14521 atattgtatg gattcagaat tgccatcccc aacaggaagt ggagccggaa gcttgaagta
    14581 caagggagct ggcagtgatg agttctatcg tatgtttaaa ggtggttttc catcaaaaac
    14641 agcaaaggaa ctgggtgcag gaaatgatac agaagcatgg tatgcaacac aattagtttc
    14701 atgggtttta gccggaaact ttaaagtttc acagattgta tggtcacatc caaaccatac
    14761 cgccgcagaa acagctcgtg taaaaaaagc atttgaaaaa atttatgact atgctaagaa
    14821 tggaaaagat acgcccaaca ctgagttttc tatcacagct tctaagacag ctgacgaagg
    14881 aaaatatcac actttcacat ataaaacagc atcaaataag actggtaatg cgaagctcac
    14941 ttttacatca gcaaaaccag ctggtatgaa gatttatgat gcagatggta aagaaattac
    15001 taacaacact gtgaaattaa attcaagttt tactattaaa gtacctgtga ctacaccaag
    15061 tggtactttg tcatttaaag gtactgctaa tgttagcaca accaatcctt ttacttttga
    15121 tggacgaggt gtgtatcaag atgccgtagt tatgatcact acttcagaaa caaaggactc
    15181 taaatctttg agtgctaaat ggacaagagc tgttggtcag gtaacagtta agaaagttga
    15241 tcaagatgga aatgctttaa aaggagcgga gtttactcta actgacaata acaatgctaa
    15301 atctgttaag acaaccggag atgatgggtt agctacgttt gatttgcaac aaggcttaac
    15361 atacagctta gaagaaacaa aagttcctga aggttatcaa ggttcattta aaaaggatgg
    15421 tattactgtt aataccgatg gacaagaatt tagttacact gctacgaaca caattaaaaa
    15481 aggtaaagta aaagttaaaa aggttgacca agatggaaaa ccattagcta atgctgaatt
    15541 taccttgacg gatgattcat cccaaacaca agcagaaaaa acagatgaaa acggacttgc
    15601 aagcttcgat attgtagcaa acaagaccta cagccttgaa gagaccggag taccaactgg
    15661 ttatacaggc gtattcaagc aagaaggaat taccgttaaa gatgatggac aaacttttga
    15721 atatacagct acaaacactt tgaaaaaagg tttagtaaaa gttcataaag tcgatcaaga
    15781 tggaaaacct ttggcaaatg cagagttcac tttaaccgat gaaaaaggtg caacacaagt
    15841 agaaaaaaca gatgaaaatg gacttgcaag cttcaatatt gtagcaaaca agacgtatag
    15901 cctagaagaa accggagtac cagtaggtta cacaggtgca tttaagcaag agggtatcac
    15961 cgttaaagac gatgggcaaa cttttgaata tacagctaca aataatttga aaaaaggtgt
    16021 agtaaaagtt cataaagtcg atcaagatgg aaaaccttta gcagatgcag aattcacctt
    16081 aacggatgaa aaaggtgtaa aagaagttaa aaagacggat gcaactggtc tggctacgtt
    16141 caacattatt gcgaacaaaa cttataactt agaagaaaca aaagtaccaa caggttacac
    16201 aggcgcattc aaacaagaag gaattaccgt caaagatgat ggacaagtct ttgaatatac
    16261 tgctaaaaac actttgaaaa aaggtgtgat caaagttcat aaagtggatg atcaagggaa
    16321 agcattagca aacgcagaat tcactttgac agacgacaaa ggtgtaaaag ttgtacaaaa
    16381 aacggacagc aatggcttgg caacatttaa tattgttgcc aacaaaacgt acaaaatcga
    16441 agaaacacaa aaccctaatg gttatacagg gaaatggtct aaagaaggca tcaccgttgc
    16501 tgacgatgga caaacctttg aatatacagt aattaatcac cttgtacctg aaacagtaag
    16561 aacaggcgga atcggtacat cagccttcct tcttgtattt ctaattgcag gattgagtat
    16621 ggtgctactg tattatcgag aacagcacaa agcaaaataa aaaagtgtta atggaaaaga
    16681 agtaaatatg aaaaaagaag gctgacgtat gaaatcaaaa aaaatttttt caaatttaat
    16741 gctatttggt actttgctag gagtgggatt gggcgcaagc tcaatctctt ctagccttgg
    16801 actagggggg caagtagcat atgcacaaca aaactataat tatcaaggaa ctggaacgat
    16861 tcatatccat aaagtaacaa atactaatgg ggatgggaac cctgtctcag atattgataa
    16921 caaagggaaa gaaatagctt taccaaatgg tgtaaaacct cttgaaggcg ttgagttctc
    16981 ttatgtgaaa cttactaaag atgaagctaa atccttggga tggacggata gtgatactac
    17041 aaaaggtcaa catattgatg atgctaaagc acgattatta ctaaacaaaa aatacagtga
    17101 agtaagtaga atgaagactg attcacaagg gaatatagac atacctatac aagtaaatac
    17161 agacgattta acagataact tgtatttgat tgttgaaaca ggaacagtca aaggtgtagt
    17221 tgaagaagcg tatcctatga ttgttaacgt acctgaacta aacggatcag atgatcaata
    17281 ctttaattat gatgtacatg tctatcctaa aaactatttc caattaaact cagcagagct
    17341 tgataaaaaa gacgaagatg gaaaggctct tgccggaact gtttttgaat tgcgaaaaga
    17401 tgatggatct gatcaggttg ttggaactta taaaacagac agcacaggta aattaactgt
    17461 agatggtttg gaattaggtg actattattt cctagaaacc gatttgggaa cagatgctaa
    17521 taaaacaacc tatttactag atggtaaaaa attcaaattt agcgttaaac gtggggagac
    17581 tgctaaagta gttcttgatg ttaccaacta caaacgacct gtaattacta aggtaattga
    17641 taaagaaacc gttgatactg gcgaagatgt tcaatataca ttgaaaccat caattcctgg
    17701 taatattggt aattacgaaa aatatgttgt caaagatgtt ctaggtgcag agttaactta
    17761 tgattcatca gaagtgatcg aaggaatgac tttagatact gattacagta tcgaacgtga
    17821 cggacaaacg acaaactatg tctttactga atcaggtatg gataagcttc agaaacaatt
    17881 tgatgcaggg gatcgcttct ttacaatttt gatgaacgct catactaata gtaaagctaa
    17941 aatggataca ccacttaaga atgatgtttc aacgatctac aacaatggat atgaacccga
    18001 agacgaaaca ccagttgatc acgcttatac aatcattggt gatcaccaat tcattaaaac
    18061 agatggagat acaagcaaag cattgagtgg agcaaaattt gtagtgaaga acaaaattga
    18121 tccgaataaa gttacttacc taaaacaaga tccaacaacg aaatctgtta cttggattgc
    18181 agataaatct aaagctactg aattcgtttc cggatcagat ggtaagttcc aaattgaagg
    18241 cttgcaatat acctatgatg tagattttga taaacaaggt aatccaacaa tcacagataa
    18301 aaaaatcaat gattatgctc ttgaagaagt tgttgctcct tcaggatata caccaactag
    18361 aaatgatgtt ccatttgtgg tatcgaatga tgatgcttct agcgaagtaa cagacatcaa
    18421 gaaccttaaa acacctgaaa cagttcgtac aggtggaatt ggaacagtag tatttctagt
    18481 ggttgcaggt gtggcaggaa cagggctata tgttgtctac aaacgagaac aacacaaagc
    18541 ttaatgaaat aaaattccct agagtgtttt tggctctagg gtttcttttt aaaaagtgtg
    18601 tgaaaagtag gtatgagcat gaaaaagaag aatatcaagt tgatcatctt aaaaattggg
    18661 atgttgctat gtttaggagt aggtctatat cctgtaatta ctcaggctta tacctactac
    18721 caaactcaac aaacactaaa ctcctataag gaaaaatatg atgaagcttc tgaaaaggag
    18781 aatgaagaag tcttcaagag atattcagaa gttaatagga aacgaaaaga cacagaacaa
    18841 acctcagtca cgattcctga agataataac aatattccgt caaatggaaa tcttcagaag
    18901 gataaattag gtgaattagt gggtgaaatt cttataccaa agattaatgt ggatttgcca
    18961 atttatgatg gtgtaaacga tttccaacta tcagcaggag caggaatatt aaccggaact
    19021 gattatccaa ctggtgggat tggaaaccat tctgttatat cagctcattc aggcattcct
    19081 aataaaaaat attttacaga tttagagttg ctcaaacttg gtgataaatt ttttattgat
    19141 atttcaggga aaaaacttgc ttatcaagta gatcggataa aaacaattct accaaccgaa
    19201 tttgaggatt tgcttcctgt ggcaggaaaa gattatgtaa cacttatgac atgtacacct
    19261 gttggagata attctcatag gttactcgtt agaggggtta gcatacctta ccaaaaaagt
    19321 attgttgaga aagaaaagaa gaaagaagat ttaaaggtaa aggcgcagat gaactggtat
    19381 cctatcgtag ttgtaggggt tttattaata atagctgtga tagtcattat taaaaaaata
    19441 gtagcaaaga gacgaggaat gaaaaatgaa gaataaaata ctactttttt tgactcttat
    19501 ttgcactttg gttttaacaa tttatcctgc aaatcagctc tatcatcaat tcaaaacaaa
    19561 agagaatgtg gcggaaatcc aagagaaaat tgagaaaaaa caaactcctt caaaagagga
    19621 agtggcaaaa aaagtcgaac aaataaaatc gaacagcggt aaccaaggat tagacaatac
    19681 tcagaaaaca gataaaggag tagacaatac tcaggaaaca gacggtggat catttgatat
    19741 tcgtgaagct gaaggaaagg tcaatccata cacaggagat gttttttcaa aaaatcaaac
    19801 gaaagatttt gaaccatcta cctattcagg agcggttggt tatgtctata ttccaaaaat
    19861 aaatgtgcta gagcttttgt ttctaggcgc ttcacaggag catttatata gaggtgtggc
    19921 acaaatgagt gctactagtc tacctttatc aggtaaaggt catcaaacag taatagcagg
    19981 tcatcgtggc ggttatacgt caccacaatt tctttatgtt gatcaattag tagccggaga
    20041 ccacatttat atagattatc ttggtgaaaa agcagtttat acggttttcc gaacagacgt
    20101 tattagaaat tacggtcaga attcaacact gactgatatt cctgatttag atatgttaac
    20161 actttatact tgtcatccat atcctcagaa ctacgatcgt ttattagtta gaagtctaag
    20221 agatgaaaaa gcaaaataca tcacgccaca agtaacagtt ggcaataccg catacgctga
    20281 tgccattgat agcgagtccg tttcagataa gacagaaact gttacatctt ctaatgattt
    20341 atttgaagat gttgatcgtg ccaacaagga attagatgga aataatgatc ttaatattcc
    20401 ggaacaaggt agcgtagaga tgcaagacag aacgtttgct gaaaaggcaa gttcaatatt
    20461 tacaggaatt accaataaac atcagaaaat gcttttagtc atttggactg taataggagc
    20521 attagcaagt ctaaaggttc ttattctttt aatcaaagag tttcgaaaag cataatgaag
    20581 attataaaaa ttgatagagt acagtaaaaa gggattgata ctattattga tctctttttt
    20641 attttgaata gagctagatt cacttttaaa taaaatcaaa actgatctta attctaaaga
    20701 aaaagtcaag aaaaaaatac aataaatttt tttatcagaa tatttatcag aatattatta
    20761 gaaaagataa actcaaaagc ttaatttaat agggttattg gttgtattta gaaataatgt
    20821 atggaaaagc tataaagcaa tagataagat gtagaaaatc ttatttgtga atgaaaaaca
    20881 agtcatcgta aaatattaat aggaggtgta gtaatggtgg gagtatcaaa aacagcttta
    20941 gactcattag ctaaactatt tagtagcagg tttgatggtg cagaatttgt tgaatatcta
    21001 agccaaaacg aaagtggaaa aactctgatc gaaacaatga aaaaagacta tttgaatgaa
    21061 aatgactttc cgacatggac gaatattgaa gatgctacat gggatgtagt atgtacgata
    21121 tattctcctt gggtaacgat tgctgatttt gttttagata aaattggagg ggatacatac
    21181 cctgatgcca aatctcttgc tacaagtgac tttaaaacgt tctatggtta tctaataggt
    21241 ctaataggca atggaataga tttgaaagtt acgccaaaac aaacacctga agaaattgaa
    21301 aaagaaaaag gagaggttca aatggcttat gcaaatgaca gagaaaaagt aaaattattg
    21361 gaaggtaaat tatctgaaga agaacttgga aaaatggtgc aagaaatcaa tgaagacagc
    21421 aactattttc ctgaattgaa ggtatatcaa aatgacgaag atttctttaa tgaaatgttt
    21481 agtaatagtc catgggaagc agtaagtaaa acaaatttag gagaatggaa ctttcaagat
    21541 gatctagttg catttagcaa tgatgggtat ttacgatcat attcagaaag tgagtataga
    21601 gctgaattaa aaacgtatga aacggatatt ctgatgagct atttaaatct attagagagt
    21661 ggtcagggag agttaagaga taaagttgaa gaactattaa aagaagagct atagggatta
    21721 tttcaacata aacacaagca gaaaactacc aaacaaaacg taccatgcaa aaagtcggtt
    21781 aaaccggctt tttgctctat tatgagagga gatcaaacca tgagtgcaac aaccgatatg
    21841 gctttagaag cagtaataaa agataataca ggaaatgtcg aaaatgtaga agactttagc
    21901 gatattgcaa tcaatctttc agcatggatt tcagctgtag gagaagaaaa tatcgaattt
    21961 gagatcaaga atatggatga cttaaaagca aaatttcctt ttattgcatc gaaactaagt
    22021 caagttgccg aggataaatc gtggaatgaa ttagttagtt tttccgaaaa aatttatgat
    22081 attacgcaag aagatgagtg tgtttcatgg gaaatgaata atcaatacaa gaaaattgac
    22141 gctttcgtca aatcacgtat agaaggaaca gcttttgagt ttgaaggtat agataccgca
    22201 gataattggt taaataaaga atcctttatt aaaagtatag cggatgatct tgaaaaacaa
    22261 tggtctaagg aagactatgc agagtaccta aaaacagaaa gagatacaga agtgaaagaa
    22321 aaactcactg aggtatgttt ggatgaacat attgatccca atatcgtaaa acaagaagac
    22381 tatgaagtgg ttattgataa agttcctact gattttgagg aagttgtaaa gtatatagtt
    22441 gaagaagcat tatcgcaata ctcttcagat gaggaagtgg acgaagaaac tgttcgtacc
    22501 agagtttggc aactggtttt tgatgacaaa gattataaat atttgagtga tcaaaactcg
    22561 gttaattgga gtgtacgatt attaattgat caaaatgata ttgaatagag ctgaaaagct
    22621 ctattcagat tgtagacaaa aaggagttgg taattaagcc actccttttt gttagtcttt
    22681 gcataatttt gaaataaaaa gccataggct tttttgtttt ccatagccat gtggctagtt
    22741 tttttaaatt catggcagca aacgtaagca tcgtgtgcgt ggcgactttt tcaagacctc
    22801 gatatttcgt ccaacgcata ccatgctttt cttttgcatc tgcaaatatt cgttcaatgg
    22861 tttgttttct ctttttgtaa atttctctat tcaacgccgt atggcgttgg tgttcaagtt
    22921 catccataag atcagcccaa atatgtcgag tcactatttt ttgatgtttt gtactcaaag
    22981 tacaattttc aagtaatgga caactcacac agaattttgg atttgattta taggttcgat
    23041 aaccatcacg agtgatagtt gaaaataaca acggttgatt attgggacat aaatagcagt
    23101 caaaataggc atcataaaca aagtcttttt tacgaaacat tcctttcact ccatgaggtg
    23161 cagtatatgg aagaatcggg tagagtttca agtgtgacag aaaatgaaca aatttaggcg
    23221 tcttatatcc tgcatcagca accacacgct ttatatttgg aaaagctctt ttcgactgtt
    23281 taaccagatc gattgcgact tggctgtcat gcacatttcc tggtgtgatt aatgtagata
    23341 agataaaacc gttatcttca caggaagtat gtgcagaata agcaaattgt ttttctcgtt
    23401 cacctttaac atagtaacca ctttctggat ctgaatgact tattttttga agactcaact
    23461 cactttctgt gtcccatgta aaaggacgtt ttcctacagc aatacgttct ttatttattt
    23521 catcttctaa ttcatcttga taagcttttg cttgaccgta ggtcatctct ttcgtgaact
    23581 tatgtttatt ggcatttgct ttgatatgag tagaatcaat ataaaggttg tcttccgtaa
    23641 cgaagccagc ttttacagct tgttctagaa tatgagagaa aatatcttca aataaagttg
    23701 tttctctaaa tcttcgaaca tagtttttac caaaagtaga gaagtgtggg ataggatcct
    23761 cgaatgaata acctaaaaac caacggtaag caacatttgt ttcaacgtct ttaattgtct
    23821 gtctcatcga acggatacca aataaatatt ggataaatac taatttaatt aagaccactg
    23881 gatcaatact gggacgacca taggttgaat acgtagattc tacaatggga taaatgaaat
    23941 caaactgaat cgcagcatca atttttcgaa ctaagtgatc tttaggtaca agatcatcaa
    24001 ttgttaacat agcaaattga tttctacctt caatcgattg ttttctcatc attagaatca
    24061 ctcccagaat agtttataca attagtataa aacaaaaaac tgcaaacaac actccaaatt
    24121 taggagtttg tctacagtct gaatagagct gaaaagctct attttttttg ataaaaaaac
    24181 tagctatttt attaaaaata atttggttca aaatttattt ttttaacttg ataatttttt
    24241 gccatccttt tttaaattg
//
LOCUS       DAXWLG010000026        23484 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXWLG010000026 DAXWLG010000000
VERSION     DAXWLG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.026"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            82..1509
                     /locus_tag="ACGE4T_000812"
     CDS             82..1509
                     /locus_tag="ACGE4T_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386115.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1601..1966)
                     /locus_tag="ACGE4T_000813"
     CDS             complement(1601..1966)
                     /locus_tag="ACGE4T_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8386116.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1963..2328)
                     /locus_tag="ACGE4T_000814"
     CDS             complement(1963..2328)
                     /locus_tag="ACGE4T_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8386117.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2520..2768)
                     /locus_tag="ACGE4T_000815"
     CDS             complement(2520..2768)
                     /locus_tag="ACGE4T_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386118.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2767..2895
                     /locus_tag="ACGE4T_000816"
     CDS             2767..2895
                     /locus_tag="ACGE4T_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFF8386119.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2952..4646
                     /locus_tag="ACGE4T_000817"
     CDS             2952..4646
                     /locus_tag="ACGE4T_000817"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFF8386120.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4713..5042
                     /locus_tag="ACGE4T_000818"
     CDS             4713..5042
                     /locus_tag="ACGE4T_000818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386121.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFL
                     TWLDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLP
                     RGYEKGV"
     gene            5420..6553
                     /locus_tag="ACGE4T_000819"
     CDS             5420..6553
                     /locus_tag="ACGE4T_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFF8386122.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6584..6904
                     /locus_tag="ACGE4T_000820"
     CDS             6584..6904
                     /locus_tag="ACGE4T_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFF8386123.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6933..7397)
                     /locus_tag="ACGE4T_000821"
     CDS             complement(6933..7397)
                     /locus_tag="ACGE4T_000821"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386124.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7528..8781)
                     /locus_tag="ACGE4T_000822"
     CDS             complement(7528..8781)
                     /locus_tag="ACGE4T_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8386125.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8810..9127)
                     /locus_tag="ACGE4T_000823"
     CDS             complement(8810..9127)
                     /locus_tag="ACGE4T_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8386126.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9153..9473)
                     /locus_tag="ACGE4T_000824"
     CDS             complement(9153..9473)
                     /locus_tag="ACGE4T_000824"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8386127.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9937..11352)
                     /locus_tag="ACGE4T_000825"
     CDS             complement(9937..11352)
                     /locus_tag="ACGE4T_000825"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8386128.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11369..12685)
                     /locus_tag="ACGE4T_000826"
     CDS             complement(11369..12685)
                     /locus_tag="ACGE4T_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8386129.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12835..13533)
                     /locus_tag="ACGE4T_000827"
     CDS             complement(12835..13533)
                     /locus_tag="ACGE4T_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFF8386130.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13822..14815)
                     /locus_tag="ACGE4T_000828"
                     /pseudo
     CDS             complement(13822..14815)
                     /locus_tag="ACGE4T_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14896..15729)
                     /gene="msrA"
                     /locus_tag="ACGE4T_000829"
     CDS             complement(14896..15729)
                     /gene="msrA"
                     /locus_tag="ACGE4T_000829"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8386131.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDTDYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15963..16706)
                     /gene="truA"
                     /locus_tag="ACGE4T_000830"
     CDS             complement(15963..16706)
                     /gene="truA"
                     /locus_tag="ACGE4T_000830"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8386132.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(16897..17532)
                     /locus_tag="ACGE4T_000831"
     CDS             complement(16897..17532)
                     /locus_tag="ACGE4T_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8386133.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(17679..18899)
                     /gene="sstT"
                     /locus_tag="ACGE4T_000832"
     CDS             complement(17679..18899)
                     /gene="sstT"
                     /locus_tag="ACGE4T_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFF8386134.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            complement(19241..19918)
                     /locus_tag="ACGE4T_000833"
     CDS             complement(19241..19918)
                     /locus_tag="ACGE4T_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFF8386135.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(20169..20711)
                     /locus_tag="ACGE4T_000834"
     CDS             complement(20169..20711)
                     /locus_tag="ACGE4T_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFF8386136.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20716..20943)
                     /locus_tag="ACGE4T_000835"
     CDS             complement(20716..20943)
                     /locus_tag="ACGE4T_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFF8386137.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(21022..22902)
                     /locus_tag="ACGE4T_000836"
     CDS             complement(21022..22902)
                     /locus_tag="ACGE4T_000836"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8386138.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            23254..>23484
                     /locus_tag="ACGE4T_000837"
     CDS             23254..>23484
                     /locus_tag="ACGE4T_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8386139.1"
                     /translation="MDFIQELECTFHKKFTHSILLKLLIHTACLIERTLINGHELKII
                     SEDDTRPSHETIFHVKKAFKNIETEFGITVSYD"
BASE COUNT         7288 a         4823 c         3968 g         7405 t
ORIGIN      
        1 aattaactga tgatttcaaa aagaaaacat gtcataatat ttcccatatt gtgcaaaaaa
       61 atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag
      121 ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat
      181 ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt
      241 gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa
      301 catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact
      361 tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc
      421 atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc
      481 gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt
      541 cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt
      601 gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa
      661 gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt
      721 gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat
      781 gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc
      841 tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg
      901 gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta
      961 gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt
     1021 gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa
     1081 ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc
     1141 agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt
     1201 gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc
     1261 acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc
     1321 gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag
     1381 cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat
     1441 gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa
     1501 aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca
     1561 tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta
     1621 cgggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg
     1681 ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa
     1741 caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct
     1801 cgctgactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga
     1861 ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa
     1921 gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct
     1981 tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat
     2041 gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat
     2101 gatatcagca acgactagta cttccaagct taacaaaata taacttgcta gcagttttct
     2161 tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa
     2221 aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa
     2281 aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta
     2341 ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt
     2401 taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt
     2461 ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct
     2521 tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt
     2581 ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt
     2641 acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc
     2701 atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg
     2761 tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc
     2821 gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa
     2881 ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg
     2941 gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg
     3001 cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt
     3061 cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt
     3121 ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc
     3181 ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg
     3241 agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg
     3301 acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca
     3361 ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa
     3421 aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa
     3481 acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat
     3541 tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg
     3601 tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga
     3661 aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa
     3721 caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag
     3781 aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta
     3841 cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg
     3901 cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca
     3961 aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc
     4021 atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt
     4081 catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctatcg
     4141 tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt
     4201 atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg
     4261 gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga
     4321 atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata
     4381 tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc
     4441 ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga
     4501 tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta
     4561 ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc
     4621 gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag
     4681 taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac
     4741 cgcagatgca agcttttgaa caccgtttac caagaggttg tgaaaaagct gccctgcatc
     4801 aagtaataag aacacccaag ctaaaactgc ttcttatgtt tttgacttgg ttggacttat
     4861 taccgcagat gcaagctttt gaacaccatt taccaagagg ttatgaaaaa gctgccctgc
     4921 atcaagtaat aagagcagcc aagctaaaac agcttcttat gtttttgact tggttagact
     4981 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaaggcgttt
     5041 agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg cataaatttg
     5101 acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat gtctgtttct
     5161 ttttgaaaac aactattttt tcaacgttta ctctcttgcc aatccaacat catctaacgg
     5221 atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc tctccgaaca
     5281 caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag
     5341 gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca attttatgaa
     5401 tctgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat
     5461 cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc gtatcacagc
     5521 aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag ttttggaaaa
     5581 agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat ctcaagttca
     5641 aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg gtcattgtca
     5701 tttctgcctg aatcacatgg aaagccaatg tgatgaatca aatccagatc agttatatgg
     5761 cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag cggaatatct
     5821 aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgatc ttcctgatga
     5881 aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg aaaacgcagg
     5941 agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc tattcgctca
     6001 aaaattcgct gttatggctg gagcagagcg cgtaatcgca gtagatccag tacagcaccg
     6061 tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag acacggcaca
     6121 agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta tcgattgtgt
     6181 aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa gcttacaaag
     6241 cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg gaacagtcca
     6301 agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat tttttatgcg
     6361 ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa aaatttacgc
     6421 aatgattgca gaaggactat tcgatcctac acaaatcatt actcattcgc tgccactatc
     6481 agaagcagca caagcatatg agatctttga caaaaaagaa gataaaaata taaaagttgt
     6541 tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca aaatcaaacg
     6601 aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag gacgtcgctt
     6661 ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt tattaaccgg
     6721 gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc gatatggcac
     6781 ctatttttat ttggctggtg ctattttctt gacgattcgt cctattctca gaatcgtaag
     6841 gaacattttt atttacgacg aaaaaaaagc aatgaagaga acaagcaaaa aaaaagaggc
     6901 atgagaattt ttcaaatctc atgcctcttt tttcacccta tcgtatcact ttgatacaga
     6961 cgagcggtcg tcccactttc ttccttgacc attgtcaaaa atgtccactc gtatcgttca
     7021 taaaacgtgg tatgatctgt aattaagtaa agtttttcgt acccaagttg atgcatttct
     7081 tttctgattt ccgctaatat ccgcttggct atcccctgct tacgcttttc cggttcaaca
     7141 aacagggcac aaagatttgg tgtcagatca gggcggttat ggaagtcgtt ttcaatgacg
     7201 cccgctcctc cgacgatggt ttctttttca tctaagacca cataccattg aggaatactt
     7261 tccttattag cgattccagt acgaatactt tcctcataca caacagcagg aactccccat
     7321 tttgatgaaa accaattcgc gctttgtttt actaattctg ggtgttctct taatttgatc
     7381 cagttatatg taaccaatgt ttaaacctcc gactaaaata aaacgtttac aaaaattata
     7441 caagtcttta tgaaaattag caatgacaac aatagtgatg taaaaaaaaa acggaaaaca
     7501 atcacttgtt ctccgttttt tgattaatca agcatctaaa gcttcttttc ttctttcttg
     7561 gatcgtagct accttgacga acggtagata gatcaaaata gaaagaacca aattgacagc
     7621 ggctagtaca ccacctgcaa tactttttgt tgccaataca ccgccaataa ttggtggagt
     7681 tacccatgga ggcataacgg tagctgctgg cactaaacct gtagaagtcg caaagtaagc
     7741 aacactaact aagaccattg gtgtcaaaat aaatggaatg aacatgatcg ggttcaacac
     7801 gataggtaac ccaaacatcg ttggttcatt aatattgaag acacctggcg caatcgacaa
     7861 gtttgttaca accatatatg gtttgttttt acgtcctact aaataaatag ctaacagtaa
     7921 acctaaagtt gcacctgttc ctcctaagtt gacaaagctg tcaaagaaag gtttgttcac
     7981 gataaacggt gcgctatgcc cagctgcgat tgcattaacg tttgcttcaa tggctggcgc
     8041 attgattgtt tgcatcaaag gatcgaccat attcgctccg tgtaaaccaa agaaccataa
     8101 gaatggtgtg ataaatgcaa gcagtaaggc agatggataa gagtttgcaa gtcccataaa
     8161 tggttcttgg accactgtat agaaagaacc tacgatatca tcaacaccga atgcagcaaa
     8221 aattgcagca actaaagcaa acgcagagat agtgatcatt gctggtagta atgaagcaaa
     8281 tgcttttgca actgccggtg gcacaccatc aggcattttg atgatcaatt tttcatttcc
     8341 gcttaaacgt acaaagattt ctgttgaaat aatcccaaca ataatagcaa taaacaaacc
     8401 gtttgagctc atacctaatg ctccgcctaa agcgaagaaa gagcctaaag aaatcacacc
     8461 tgcggaaaca ccgtcttttc ggtagccgtt agctaaatga taagcaagca agaaagcaat
     8521 caagacagaa agaatggcaa atgtaccgtt ccaagcagca gccccgaatc ctttccatga
     8581 ttcaccacca aaaattgagt tcatcaattc ttggaacccg ggaatcggta agttattgat
     8641 cattactgct aatgctccta agatcattaa cggcatactc accataaatg agtcccgaat
     8701 cgcaactaaa tgccgttgtg caccaatttt tgatgctaca ggtataaaat gtttctccat
     8761 aaatgcaaca aatttatcca taaatcttcc ctcttttctt tactgtagat tacgcgttca
     8821 atcgacgata cagatcaatg atctcttttg ctaaatctgt aaaagctata gatgtcatca
     8881 gatgatcttg actgtggacc gtcaaaagcg tgacttcaac gtgatttcct tgtgcttctt
     8941 gtgtcagcat ctctgtttga gagtgatgtg cttggactaa agattcctct gcatctttga
     9001 ttttttcatc tgctaaagtg aaatcacctt ttttcgcagc agcaatcgct tccattgcat
     9061 cgctttttgc atttccgcca aacatgatca gacccatgat ggcttctaga tttttttgat
     9121 cttccattta ttgtatcctc cgataatttt tattatttgt ttattagttt ttctgcttgg
     9181 tcgagtactt tttcaccatt catcatgcca tagtcagcca tattgattac atctaaagga
     9241 atgcctttag gtgctagctt ttcagcaaat tgattttgca taaagcgtac ttgcggtcct
     9301 aaaagtaaaa cgtctacatt tttagattct agttgattat ccgcatctga agcagataca
     9361 gcaaagatgt ctgcttccat tccttttgct tctgctgcct tttgcatttt tgtgactagg
     9421 agacttgtac tcattccagc tgaacataca agcatgattg ttttcttttc cattttaaac
     9481 acgttccttt ctatacttac acttgtatat aagcaagtag cgtgccaact ttaaaagcgc
     9541 tttaaaaagt tgatatcatc acatttaagg tctataccaa gttgcataca cactaaaaaa
     9601 gaaagcgcac actaaacaca caaattaatt tttattacct tttttctttt aacgtgcaca
     9661 aaaaaagttg tgacacaaaa cttgtcacaa cttttttcaa ataaacggta ttcaaaagct
     9721 tgcatctgcg gtaataagtc caaccaagtt aaaaacataa gatgttgttt ttactttgct
     9781 attcttatta cttgatgcag gacagctttt tcacaacctc tcaataaacg gtgttcaatc
     9841 agctgaccct gggtattagc tcaaaacgct ggattcacaa cctcttttat ttcaaataac
     9901 cagtaaatac aatgaacatc tacaaaaaac ttctatttat ttatagattt cgtcaggcaa
     9961 atccaattct ctcttatcaa tcaaagtctg ataccacgaa gcagattttt tcaatgttct
    10021 gcttcgttca tcgttcaatt cgattcgaac taatccatat cgattcttaa atgcattcat
    10081 tggtgacaca cagtcagtga atgcccataa catatagcct tcgcaatttg cgccttcttc
    10141 tgttgctttg atcaagtaat gcaaatgctg tccgataaat tcgatacggt aatcatcttg
    10201 cacgattcct ttctcatcca tatactgttc ttcatattct cttcccatac cgttttctgt
    10261 tatcagccaa gggatattat gataattttc ttttagatac atggctatat catacatgat
    10321 tttcggatag atttcccatc ccctggaatt attcattttc ttcccaggta aatcaaagac
    10381 ttcataatat cgttcaggat ggaaaggtgt ctcttcattc cattgataac gaggggcgcg
    10441 aacgcgttta ggataatatt gattgattcc aaggaaatct actgtatgat gctgaatcgt
    10501 ttctaaatcc tctgttgcag gatcgaaata aatcgattgt ttttgacaca actccaggaa
    10561 ctcttctgtg tagtatcctt ttaccatcgg atcaaaaaat actcgattat aaaacaaatc
    10621 atacatataa gcagcttttt ggtcttctgg agaattggat cgagcataga ccatttcagg
    10681 atttaaaata cagccaatcc ttccaccata atttccgctg cggtaaatct ctactgcttt
    10741 agccgttgct aaaactttat gataattcca catcatccac ttttttgtgt tttgttcatg
    10801 cggccagcga acagcatcca aatagcaacg cgtttgggga acgattggtt cgttgaatgt
    10861 aaaccagttt tttaccctat caccgtaacg atcaaaggca atttgagcat acttagcata
    10921 aaaatcaact acttttcttg aagaccagcc atcatatttt tccaaaagaa aagctgggac
    10981 ttcataatgt tccaaacaaa tcatcggttc tacgtctgct tcgatcaaag agtcgatcat
    11041 ttcacttata tgctgagcat attcttcatc tactatacag ttctcataat cagtaaaaaa
    11101 acgagcccaa ttgatagaag tccgataatg tgtcagatgg atggatttca tcagatccac
    11161 atcctgctgg tattgttcca taaaattagt tgctacggct gggccatacc cattatgcca
    11221 aacaaaatgt tcatttttat accagctatc tataaacgaa tcctgtcctt ctttttttcc
    11281 tttccaacct tccgtttgcc aagcagaaga tgcagcacca agaataaaat tttttggaat
    11341 agtaattttc atgatttcct cctgtttttt agatcccatc attttccaca cgatttgcta
    11401 cttttacaaa tggcagatag atcaaaatag aaacaataat acaaacaagt tgcgtaataa
    11461 ctgcaccgat atttcctgct gttgccagat aagcattgac gatcggcggc attggccaag
    11521 ggaccattac aactgctttc gccgcaaatc ctaaagcggt tgcaaaataa ccaatcgttc
    11581 cagtgataag aggcgttaag ataaacggaa tagctaaaat tggattcagc atgatcggca
    11641 taccaaaaat aactggttca ttgatgttga agatcccagg agcaagggat aacttggtga
    11701 tttgtttcat atcttcccgt tttccaacta acaatactgc aatcagtaaa ccgatcgtga
    11761 ctccagaccc gcccatactc atatacatat cccaaaatgg catagtaata atgttcggta
    11821 cttcttttcc agcttcaaaa gcttccgtat ttacagcaat tgccgctaaa agcaacggtt
    11881 cccgtatcgg cttcaccatt tgatttccat ggataccaat cacccaaaag acttgcgcaa
    11941 taaacatcaa tagcaaaata cccggtaacc cttgtacgac accttctaat gggcgttgta
    12001 cgacattgta aatgatatca taaaggtaca ttcctgtaag ttctttgatg ataaatcccg
    12061 cagccgcgat acccgataca gtcagaatcg tcgggaataa agcagaaaaa ctagcagaaa
    12121 cattcgttgg cacggtatca ggcattctca gctgtagttt cttctgtttc ctcaaccagc
    12181 aataaagttc aatagaaagg atggagacca acattcccaa aaataaccct ttcgtatctg
    12241 tatattgttt agccaatacg ttttctacta taacagtctg attttcattc actaattcat
    12301 aaacagttgg attaacagat aaataagaca tcactgcgat gataccaggg aatacaccat
    12361 ccactttatt cagcttggct aattcgatcc ctatcagaaa aacagcatag atggtcaaga
    12421 aacttagtgt gacatagctg atggcagatg cgataggctt taattcttct aaaaaacgca
    12481 agaaacttat ctgtgctaag ccattttccg agtcaaagac cattgctgaa aacaatgtgc
    12541 caaaagcacc cgtaataatt accggaatca atgcagcaaa tgcgtttttt atcaccatga
    12601 tataccttaa attattgatt ctcgttgcca cgttccctaa aacattggct aatttatcca
    12661 taaaactcat tccagatccc ctcattcctg cttcttctcc ttgacgtcat ctctttttgt
    12721 ctattagtca gcgcttacat ttcgatgaac agaaactatt tactcctctt ttttaccaaa
    12781 tataaaacaa aaattcatct gatgtattta tcataaaata catcagatga actgtcaatt
    12841 agtttctttt tctcctatta ttttctgtaa ttctctccga tttattgcca tatggacagt
    12901 cattgcatca aacgcagcaa gtgcattccg cctttcgatg gcctgaacga tctctgtatg
    12961 aatatcgatc gtctcgattt tcagatgctg gatatcataa tttttattaa acaaagcaat
    13021 tgattcatta atgattggga taatatgcag caaggctacg tttccgctag cttctgcaat
    13081 catcgtatga aaatcgatgt ctaattgaac atgttcttgt gtgccttctc gaaaagaagt
    13141 ttcaatcttt tgcgttaatt ttttcatttg gacgatttga ttcttcgttg catattccgc
    13201 tgccaaacct gccatcttag gctccaataa atatctcagt tcaaataagt ctcgtatcat
    13261 ccgatcctga tcttcaatca aagaaaaacc gagtgggtca ttggcaatcc ctcgcttatc
    13321 actgacaaat gtccccgctc cttgccgaac ttcaaggata ttcctggaag ctagagcccg
    13381 cacagcttct cgcaccgtgc ttcgccccac ctctaaataa tcggacaatt catattcatt
    13441 cggcagttta tctcctactt gcaactgcat ttcctttaaa tatctaagga tcttgtcagt
    13501 agttacttct actagggttt tattttcttc caaaccacat acctcctttt catctgatgt
    13561 attttttcgt aattattata acacgtttga atcatctgat gattttttgt atacaaaatt
    13621 gattaatccg ttcatagaca tacaaacgaa gaagatattt accataaaaa agactaggat
    13681 aaaattcact cctagccttc gatcattttt attttgaacg acaaaaaata tgatgttcaa
    13741 taacagacga tcttcattcg attcttgatg tcatcttttg ttttttttaa tatatggata
    13801 aaaagtttga ttacatcgtt tctattctag aatttggtcg attctcaatt cttcttctgg
    13861 tgagaaatct aaattatcca tcattcgaac actttcttgt aactgactta accgacttgc
    13921 tccgaccagt acacttgtaa cagatttttg tcgcaaattc catgcaaggg ccatttgcgc
    13981 taatgattgt cctcgtgata cggcgattgt ttgcagtgct ttgatttttt ccagagttga
    14041 tccaacttgt tcttctgaaa ggaatggtat ttccttacga tgtgctctag aatcttcagg
    14101 aataccatgt agataacgat ttgtcagcaa cccttgagca agcggactga acgtgattgc
    14161 ccctagccct tcttcttcta atactggaga tagatcgtct tctagtagtg cacgagaaaa
    14221 catattgtag cgcatctgat gaataatgaa cggtgcttcc tttcttttta aaatttcagt
    14281 catttttttg tatcttctcc attatagttg gatataccaa tatataatgc ttttccttgt
    14341 cttacaagtt gcatcaacgc ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga
    14401 tgatgataga agatgtcaac ataatctagc cccattcttt ggaaactttg gtcacaactt
    14461 gatatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat atgataacct
    14521 gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct aaaaatacgc
    14581 ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag gtcgaaatgg
    14641 gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat aggatcataa
    14701 tcaccaaaat tttgccataa tccgagagga gagataaaat cggtaatttt aaacctgaat
    14761 gaccggattt acgatattcc attttttcat aacgtgcact atctgcaata tacaattttt
    14821 ctcctcctca tcaaatcttt ttaaaacgct ttcttgtttt ctatttttag tttactacat
    14881 tttaaagaaa aggagttaat caaaatgatt ttttcgtata tttttttgcc atttgttttt
    14941 tactcgttga tagatcagaa attgattacg tgcttgctgg atttttttat agcgtttcgg
    15001 ttgttttttg taaaattgct gatgatagtt ttctgctggc caaaacgtac cggctggttc
    15061 gatttttgtg acgattggtt ggtggaaagt tcctgaactg tctagtttct gttttgattg
    15121 ttccgctaat tctttttgtc gttcgtcttg ataaaaaatc actggacgat attgcttccc
    15181 tctatcttga aattgtccaa atgtatctgt gggatcagta atttgccaat agattgtcag
    15241 caactcgcta taacttattt ctcttgtgtc ataaatgatc tccacagctt ccacatgacc
    15301 tgtgtaacca ccacttactt gatcataatt cggtttttca acatgtccgc ccgtgtatcc
    15361 tgacaaaaca gataggatcc cttttttcga ttcaaacggc tcgaccatac accagaaaca
    15421 tccccctgca aatactgcac gttcttgata gtcagtatct tgcatggcat gcttggaaaa
    15481 gtcatatttt tgctccttat caaatccttc cgtaatcaat aaataaaagt ccattacatc
    15541 aggcgtcaga ttcattcgga tagctaatgg acgtagtgtt acttctagtt ttgataactg
    15601 ttctttaacg gatatatttt ctcttgtgtc attctttgtc tgaatcaata cttttcgttc
    15661 ccaatctcgt gtattcggat tcaaaatcag attataaagt gtttgaatga atatttgttc
    15721 ctcatccata gattctcctc ctttccgaag aagacaagct agtgacttta gctgtaattt
    15781 atcataaagt gaggaaataa tcgattgaat cgtatttatt caaagaagat aagcagcttc
    15841 agatgatata agatgatctt tatcttaaaa agcatactct atggtttggt ttaactaaaa
    15901 aacgacctct gaaaaaaggg atgttctcca attctcagaa gtcgagtaaa gatgattctt
    15961 ttttatgagc gataataaac ttcatccaaa aacagacctt gtgcagggac ggtttctcct
    16021 gcatgttgcc gcactttgct ttcaaatatt tcatcgattg tttctatcgg catggcacct
    16081 gtgccgattt ctagtaatgt ccccatgata atccgcacca ttttatataa gaatccgtct
    16141 ccgacaaaag taaaatgaag catttctccc tttcgttcga tagataattc atcaatcgta
    16201 cgcactgtgg atttttttga ttttttcaat gcagaaaaac caatgaaatc atgtgtaccc
    16261 actaattttt cgcaagctgc attcagcttg tccatatcca actcttgagg gaaataaaag
    16321 ctgtggtttc gctcaaatgc tgaaggaatc acgttgttcc aaacataata gctgtatttt
    16381 ttgcccaccg cattatatct tgaatggaag cgctcaggca tttcttcgat tcttttgatg
    16441 acaatatccc gcggaaggta acgattcata aagtcgatca tttctgccag tccaatttct
    16501 gtgtttgttt taaaatttgc tacttgtcct cgcgcatggg tgccagcatc tgttcttcct
    16561 gatccaataa tttctatctc ttcatttgtc atctgatgca atactttttc gatttttcct
    16621 tgaatggttt tatcgtcatc tccaagtctt tgccatcctg aatatctttt tccgtcatat
    16681 tcgattgtca acttgatgtt tctcatattt tagtggctcc tctgtcttcc atttggtctt
    16741 ctctctacct ttttaagatt gtagcacaaa atatagctcc ttgcttctct aagacagaag
    16801 ctatacaaga aaactgtgga aatatatatt tcagaaaaac aaaaaacagc caccggatga
    16861 agtatccgct gactgttcaa tttgacggct caagttttac tgtttgagat gcaagatact
    16921 caaccaggca atgggcacat aattaaagaa aaaactgatt ctttggcaaa aacctatttt
    16981 attcagtaat ggccaagaat ttatagaagg aattcttgcc agtccataaa tcgccgcaaa
    17041 aagcaaactg atcacagaca gtccaaggta taagtggctt acgtccattt gcccattttt
    17101 tcgatagatc aagaaaagaa gaactggaaa caataaaaaa ccactgtaac ctaaacctga
    17161 tccgatatta tggacccatg tcgagacatt ccacgaagat tcatgggtat tgatactgaa
    17221 caaacctgta aagatacaat cacctatacc atagaacgcg atggctgctg taactagggt
    17281 agctaacaca cgagatgtac ttacaaatgc agcatacaat gcaggcaatg acaggatgaa
    17341 aaagatccct gaaaccacag accaaataag aaacgctttt cttacaggac tggaaacctc
    17401 tccaaataca ctgatcacgc tcttcatttg atctaattca ggataataca atcctaatac
    17461 gtacggagtc aaaaaatcac tcagcacgcc taataataaa aagaaaaaac caaatttacg
    17521 taaaaaatcc atgatacgcc tccaataaca attacctatg cttttaattg ttattataaa
    17581 cgactttatc ttgatttaac agaaacctct atttacacga acatctatat aactcattaa
    17641 aaactcgaat cattttaaaa atactaaata tctaacgttc aacgttcact taatccatct
    17701 aaacaatctt tcaatttgat tttttcccca cttcttttac gcgcgcctag ctctcctatt
    17761 gcagtaaaca acagatcact tgacgaattt aaggcagttt ccattgaatc ctggataaca
    17821 ccgataataa atccaatacc aacgatttgc atcgcgacat cattagagat accaaacaaa
    17881 ctacatgcta atggaatcaa taacagtgaa ccgcccgcaa cacctgaagc tccacaagca
    17941 gctaaagcag acaaaataca taaaaggact ttcaaaacaa atggtgcttc aatccctaaa
    18001 gaatgtacag cagtcaacgt catcaaggaa atggtaatcg cggcaccgcc catattgatc
    18061 gtagcaccta acgggatcga tatggaataa gactcttttg tcaatttcaa tctttcagcc
    18121 aacatcatgt ttatcgggat atttgcggca gaacttcggg taaagaaagc gggaagtgcg
    18181 ctttctttca aacaaaagaa aatcaatgga tagggatttt ctttaataaa caaaaacgtg
    18241 aggaatgggt acacaactaa tgcgacaaac agcatcgtac cgatcaaaac aagtaaaagc
    18301 tgcagatact ctgccaatcc agcgattcca gtcgtagcaa ttgattgata aaccaatcct
    18361 aaaatcccta tcggagcaaa tgcaataatc acttgtacga cttgtgacaa cgctatagat
    18421 atctgatcca ccaactcttt tgtcggttct gaacgtgctc gaaaagctag tcccaacaat
    18481 acagcccaga ataaaacgcc aaggtaattc gcatcaataa gagattgaac aggattttgt
    18541 gtgacattcg tcaacatagt agataaaaca ctttccaaac tttgcggtgc tttttcagat
    18601 actgtttctt gtaaagtaat aggaacagta aaaattgaac ttacaatgac tgctacaagt
    18661 gcagaaagca atgttcccac taaatataag atcaaaatag gcttcacgaa tgtcttattc
    18721 ccaattttat gcttggcaat tgacgccatc gtcaagaaaa aaaccagaat aggtgcgatt
    18781 gccttcaagc aactgacaaa caacgtcccc aatattgaaa taaatgtcca cgtggggaaa
    18841 aagataccaa acaatattcc gactaaaata ccaacaacaa tacgttgaac taacgacata
    18901 tccaaaaact tctctaacaa atgaccaccc tttcacaatt ttaacaatca ttgtatcatt
    18961 ttatctaaag aaactaaagg tgcaattttc taaaaattct aataatacaa ttggcaattc
    19021 atgctcttgc aacattcaat tttagtattg gagggtttat tcaatctcta aagaagtcga
    19081 gagaaaacta tttatcgtta ttattatagt tatccaagta aaacgtaact aagatgaagg
    19141 acgaaactag aaatttcatc cttcatctta attcttagac atagcaatca attaatattt
    19201 tccactgagt ctcttctttc tctcactctc actggtttac ttacccataa acgatttgat
    19261 taaagccatt tttcaattca ttattcgctg tctcgttaaa gtaaagataa ttatcgggat
    19321 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
    19381 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
    19441 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
    19501 ccatcttcca ctccccgttc aaaataactt gagggacttt aagaccatat aagtccacac
    19561 tcatttgatt cgtatagctt ttgtattgtt gtcccattgt ttggccccat gtagccataa
    19621 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcgact tgttcgctgc
    19681 tttctgaaga tgaagcctct acagaagaac ttgaagtaga tgtttgagtt gtttcagtcg
    19741 ttttggaaga ctctgacgaa gtagaggtat ctgctgtttc cgtacttgct gcagttggct
    19801 tgctctctgt gctagttgtt gtagtagttg caattgttgc tttcgctgtt tcttttgttg
    19861 attctttttt cgattcattc ccgttggtac aaccagctaa caaaaaagct agaccaattg
    19921 ttactaaaat ccttttttta aattaaaaaa ctcctttcga ttactcccac ttctttattc
    19981 tatcaaataa gcaatgtgat tttattgatt ttttgtattt tctcatccat cttttaaacc
    20041 ttttaaaaac taagaagcac ccgctgatgt tgtctgtaat acatcagcgg gtgcttcttt
    20101 tattttaatc ttcgcgattt tccaatttac ctcacgcttt atatttgtat ttgaagaaac
    20161 atagactttt agtcatcatc gtcttcctca tccaaatctt gtcggacagt ttttccttgg
    20221 agtctttgta cttggtagat gatttcttta ttgaaactta aaagttcaac aagcaattgt
    20281 ttcaagccat atttttcttc tttttcagct aatttgattg cccgtgtgat aaagacattt
    20341 tggaaatcaa aatcttttgc aaagttttcc aacattgttg aggctggtaa atatttgttt
    20401 tgaccatgtt cgtaaatttt tccataacgc atgacttctt ctaaagtaga cggaacgatt
    20461 tcattttcaa tcactaactc atgagagatt ttattgaacc atctcgcttc ttgatcagca
    20521 tattctgaaa aaagggtact taattcttgc gaagatgtac ccaaaacata caattgtgct
    20581 tggatcaatt tcatttcttc taatttaaaa tttgccaata catgccccac catggcgcca
    20641 gcagttggtt tatgatgatc gatatctgct tgttttgttt cttgagcaaa tttttcttcc
    20701 actatattca ctgatttacg cctcttttac ttttgttgat tggacttcat aacctaattt
    20761 ttccacaact gcagctaatt gttcagggtg gacttcatct ggatcaaagt ttgctttgat
    20821 tttgctcgca ttaaacaaaa ctttcgtatt gcttacccca ggctgttgag aaaccgcatg
    20881 ttctattttt tgcatacagc ttggacaagc tagtgtatct aattgcataa tcattttttt
    20941 catcgtagtt cttccttcct ttttcttgat agtcttattc tagattacag cttccataaa
    21001 aaaattgatt cttatcaagt tttacgattt ttcttctttg ctttcgtgtc caaaatacaa
    21061 taaacgcatc gcgttaaaga taacgatcaa gatactagct tcgtgaacga acatcccact
    21121 agccatgtaa ataaacccgg caaataagcc gatgagtaaa aataagacga caccaatggc
    21181 aatcactaca ttttcacggg tattcaaaac agttttctta gccaaacggt aagcatgaac
    21241 caacgatcca aaactagatt gcatcaagac aacgtcactc gtttcgatag cgacatccgt
    21301 tcctgatccc atagcaatcc cgatatcagc cgcagccaaa gagggagaat cattgattcc
    21361 atctccaatg aaagcaacgt gcaatccttg ttcttggtat tttttcacaa attgaacctt
    21421 ttcatctggc aataattcag catggacttc atcaatgcct aacatgtcag ctacataatc
    21481 agcagtcatc tgattatcac ctgtcagcat cactaaatgt tttgctcctg cttgtttcaa
    21541 catagctaat tgttcagcga cttctggtcg aatcacatct gacacaccaa tgatttgagt
    21601 gatttttttg tcgattgcta caataatgac tgagctgcct ctttgttgta attgatgtaa
    21661 atcttgtact tgctttggtg tgagagaaag tttgtgagcg gcaatcaatt tggtgttgcc
    21721 cgcacaaact gtatgaccat caatctcagc tgtgattcct tgtcccttga tcgtctgatt
    21781 atctttgact gctaattgtt gaaaatcaat gtctttctcc tgagcataat ggatcacggc
    21841 gcgccccaga ggatgatcgg ataacgtttc taatcgagcc actaaagcta agctgtcccc
    21901 ttcatcctct gtatagttct tgatagtgga tacagcagta gttcctttgg tcaatgtacc
    21961 ggttttatca aagacaaaag tatcgatttt ggaaaaagta tccattgctt caccgccttt
    22021 gactaagatg ccccgtttcg ctccattacc gattcctgct acattcgaaa caggcgcacc
    22081 aatgactaaa gcgccaggac aaccaagaac caaaacagtg atggctaaac gaaaatcacg
    22141 actgatcaga ccaacaagca acgcaataag taaaacggtt ggagtataat actgagaaaa
    22201 ttgatcaatg aatttttccg catgtgattt ggtatcttgt gcttcttcga ctaattctat
    22261 aattttggca aaggtcgtat catctccgac cttagttgct tcgatcgtca cgtagccatc
    22321 tgctaaaatc gttccagaaa atacgttatc tccactgatt ttgacgactt gtcgtgattc
    22381 acccgtcact gcggcttcat ccgcataccc tttcccgtca atgactcgtc cgtccacagg
    22441 aatagctcca ccagttttta ctaagacgat atcgccttct tccacgtcat cgatttccac
    22501 ttcttcggtc gttccatctt cttggacaac gctggcagtc gatggggcca tctcggtcaa
    22561 agccttgatt gattcccgcg ttttggtcaa tgttttttgt tccagataac taccaaacaa
    22621 aaacaagaat gtaacgatag ctgactcctt gtattctccg atcataaagg caccaagcac
    22681 agcaatagta acgagtaaat caattgagat aactttgact tttactgcct gatacgcatg
    22741 gattataata ggtatgaccg caataataga agctatggct agagcaatgt tatagaacaa
    22801 gacaaatcca gtaaatttcc cgataaaacc aaaaataatc aacacagctg ataaggcggt
    22861 gatatgattt cgatgctctt ggatccactt ttgtaatttc atctttctcc ctcttttcct
    22921 tttcattctt acgagactaa gtatagatac ttatccatca taaaaaattg accactatca
    22981 agtttattca aaaaggcggt tttcttttgg tatactgaat tcctacagtc attaaacgat
    23041 catatagaaa aaggaagcgc cgaaacgctt ccttaggtag ttctattttt ttattcaaag
    23101 ataagatgct ttgtcatctt acgactgaag aacaacaatc aaaaaagggc agcagccact
    23161 cattcacaag aaatatcgtt ttttgatatg ggagttgaag cagtggacaa atatctctac
    23221 ttattagctg ggaataagat ccaaaaaagc ttaatggatt ttatccaaga attggaatgt
    23281 actttccaca aaaaattcac tcattccatt cttttaaaac ttttgattca tacagcgtgt
    23341 ctaattgagc gtacattgat aaacggtcat gaacttaaaa ttatatcaga agatgacaca
    23401 agaccttctc atgaaacaat ttttcatgta aaaaaggcct tcaaaaatat tgaaacagaa
    23461 tttgggatca ctgtttctta tgat
//
LOCUS       DAXWLG010000027        23470 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXWLG010000027 DAXWLG010000000
VERSION     DAXWLG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.027"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(167..904)
                     /locus_tag="ACGE4T_000838"
     CDS             complement(167..904)
                     /locus_tag="ACGE4T_000838"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8386140.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(971..1540)
                     /locus_tag="ACGE4T_000839"
     CDS             complement(971..1540)
                     /locus_tag="ACGE4T_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFF8386141.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1668..2540
                     /locus_tag="ACGE4T_000840"
     CDS             1668..2540
                     /locus_tag="ACGE4T_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8386142.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            2882..3754
                     /locus_tag="ACGE4T_000841"
     CDS             2882..3754
                     /locus_tag="ACGE4T_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8386143.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            3751..5709
                     /gene="nagE"
                     /locus_tag="ACGE4T_000842"
     CDS             3751..5709
                     /gene="nagE"
                     /locus_tag="ACGE4T_000842"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFF8386144.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5960..6811)
                     /locus_tag="ACGE4T_000843"
     CDS             complement(5960..6811)
                     /locus_tag="ACGE4T_000843"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8386145.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            6934..7401
                     /locus_tag="ACGE4T_000844"
     CDS             6934..7401
                     /locus_tag="ACGE4T_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFF8386146.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(7664..8146)
                     /locus_tag="ACGE4T_000845"
     CDS             complement(7664..8146)
                     /locus_tag="ACGE4T_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8386147.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(8246..9109)
                     /locus_tag="ACGE4T_000846"
     CDS             complement(8246..9109)
                     /locus_tag="ACGE4T_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8386148.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            9707..10555
                     /locus_tag="ACGE4T_000847"
     CDS             9707..10555
                     /locus_tag="ACGE4T_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFF8386149.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(10726..11067)
                     /locus_tag="ACGE4T_000848"
     CDS             complement(10726..11067)
                     /locus_tag="ACGE4T_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFF8386150.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            11153..11860
                     /locus_tag="ACGE4T_000849"
     CDS             11153..11860
                     /locus_tag="ACGE4T_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8386151.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            11847..12170
                     /locus_tag="ACGE4T_000850"
     CDS             11847..12170
                     /locus_tag="ACGE4T_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8386152.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(12259..13296)
                     /locus_tag="ACGE4T_000851"
     CDS             complement(12259..13296)
                     /locus_tag="ACGE4T_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8386153.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(13743..14726)
                     /locus_tag="ACGE4T_000852"
     CDS             complement(13743..14726)
                     /locus_tag="ACGE4T_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFF8386154.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(15142..15912)
                     /locus_tag="ACGE4T_000853"
     CDS             complement(15142..15912)
                     /locus_tag="ACGE4T_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386155.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(15905..16840)
                     /locus_tag="ACGE4T_000854"
     CDS             complement(15905..16840)
                     /locus_tag="ACGE4T_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386156.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(17434..19083)
                     /locus_tag="ACGE4T_000855"
     CDS             complement(17434..19083)
                     /locus_tag="ACGE4T_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8386157.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(19278..20174)
                     /locus_tag="ACGE4T_000856"
     CDS             complement(19278..20174)
                     /locus_tag="ACGE4T_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8386158.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20167..20847)
                     /locus_tag="ACGE4T_000857"
     CDS             complement(20167..20847)
                     /locus_tag="ACGE4T_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8386159.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(20848..21630)
                     /locus_tag="ACGE4T_000858"
     CDS             complement(20848..21630)
                     /locus_tag="ACGE4T_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8386160.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(21623..22540)
                     /locus_tag="ACGE4T_000859"
     CDS             complement(21623..22540)
                     /locus_tag="ACGE4T_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8386161.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(22543..22827)
                     /locus_tag="ACGE4T_000860"
     CDS             complement(22543..22827)
                     /locus_tag="ACGE4T_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386162.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         6933 a         4953 c         4172 g         7412 t
ORIGIN      
        1 agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct ttttctacat
       61 aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat ggtcgtttga
      121 ataactattt aacaagtaat tctctttgga tattatccgt tatggattat acttcctgtg
      181 tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct atcgcaaatg
      241 ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct tcatgaagtt
      301 caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca gctcctggag
      361 agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct tccatgattg
      421 cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca acatgtcccg
      481 ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt ggaagtacgg
      541 cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc cattcatcac
      601 ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac aatacatcga
      661 ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca aaatctctta
      721 catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt gcttttaggc
      781 gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat agcttcgttg
      841 ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct gataaatcat
      901 tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat ctcttatttc
      961 atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg attcctgcaa
     1021 cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca atggcattct
     1081 cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca tttttcttga
     1141 tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga tggttcaccg
     1201 tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg ccacctaagt
     1261 caacgaacat cacttgacga ttgataaaga catttttccc tatactaata tgtttgccga
     1321 aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca ctaatgatat
     1381 tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc gctgctaaag
     1441 gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc gttactcgaa
     1501 tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct ccttttatcc
     1561 ttactttata acggacggct tatcatgtct aatacttata aaacatactt aactattcct
     1621 tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg gatattcgta
     1681 tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca gcaaaatctc
     1741 tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa gaacttggtg
     1801 tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt cgatatcttt
     1861 attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg
     1921 acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca atctctttca
     1981 tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt catttatata
     2041 gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat tttggactcg
     2101 taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc caagatcgtt
     2161 ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc catccgcagg
     2221 aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt caactagctg
     2281 aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac ttattgtaca
     2341 atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat gggatcatca
     2401 atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa gcttcactga
     2461 atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc
     2521 taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt ttaacaacga
     2581 ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt atttcttcct
     2641 tttatttctg ataattccag taaattacta taattataaa aaaagaattg acaaaacata
     2701 actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact agatcatact
     2761 aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg tctatttttg
     2821 tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg
     2881 catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg gtggacagga
     2941 aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt tgatttttga
     3001 tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt tgcaaaattt
     3061 acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg atcatgcgga
     3121 aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg accacattgc
     3181 tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt taagagaaat
     3241 cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt atctgacaaa
     3301 agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc atattcacag
     3361 cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga ttgtatctga
     3421 catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac cattttcact
     3481 gaattattca agactggtga atcatttacg actgcttcta caacgaaccc atgcgcaaca
     3541 atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta
     3601 taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa
     3661 agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa tcgtacaaac
     3721 aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt
     3781 tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg gatcggaaac
     3841 tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg cggtaacgca
     3901 gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat gtcagtggac
     3961 aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt aaatgtttta
     4021 aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga tccatctttt
     4081 gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc agctctttat
     4141 aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg caagcgtcta
     4201 gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg
     4261 cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt aggctggggt
     4321 ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt gcatcaagca
     4381 atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa atttttggct
     4441 tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc aatcatgatg
     4501 ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga aaacaaagca
     4561 aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg tgtgacagaa
     4621 ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca tgcaattttc
     4681 actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt tgcctttagt
     4741 gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca gccattgatg
     4801 ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa ctttgctatc
     4861 aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga tgacgatact
     4921 gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta
     4981 ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg tttgcaagtc
     5041 aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag
     5101 atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt cgttgcagat
     5161 gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa caccgtggtt
     5221 aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat cgcaaatggg
     5281 aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat gatgggtgac
     5341 ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg tacgatcaca
     5401 aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat cgaagtcttg
     5461 cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt atatgtagaa
     5521 gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc cgctattcaa
     5581 tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa agtccaaagc
     5641 ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac
     5701 aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag gagctgagac
     5761 aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc caactccgcg
     5821 gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac tcttcttatt
     5881 acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact aatcggcttt
     5941 ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag
     6001 aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct
     6061 ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt
     6121 cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tctttacctg
     6181 tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg gataattcta
     6241 agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt
     6301 tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag
     6361 cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg
     6421 aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct aatacactga
     6481 tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg
     6541 ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa
     6601 gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa
     6661 tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta
     6721 catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac
     6781 ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt
     6841 taagattcac agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata
     6901 agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat
     6961 gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag
     7021 atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc
     7081 aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt
     7141 ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc
     7201 caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta
     7261 gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt
     7321 gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc
     7381 ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt
     7441 ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc
     7501 taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt caagtagctg
     7561 accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca tttttcctct
     7621 ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt ttattgacta
     7681 ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg actacagcat
     7741 ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca aaggccgtat
     7801 tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca acaagatacg
     7861 ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc gtttgaacac
     7921 cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa taatgatttt
     7981 cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc tcaaatgctt
     8041 gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc acaccaatac
     8101 ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct cccacccttt
     8161 ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt acgctctttt
     8221 ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc caaatctcac
     8281 tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga gcttcgacta
     8341 acccataatc tgaagaagga agccattctg catagatttt cccccaagta tcttgtaaag
     8401 tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct atagcaagca
     8461 tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac
     8521 tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt acttgatttg
     8581 gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta
     8641 acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc gccacacctg
     8701 ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct cctctgacat
     8761 caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctactg atttctgaag
     8821 gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat
     8881 atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta gccaaagcca
     8941 atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta aagatgcgtt
     9001 ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa agtccttctg
     9061 tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata tttatcttcc
     9121 tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg aatcaggaac
     9181 caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca agcagcgttt
     9241 cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa aataaatttt
     9301 ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc cttagttaat
     9361 ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct
     9421 tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt atttatttct
     9481 aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa
     9541 cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga atgaattttt
     9601 gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt tcttaaacgt
     9661 ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga ataattggct
     9721 tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt
     9781 ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta
     9841 taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat
     9901 agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat
     9961 aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc
    10021 tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcacttta tcgaacgctt
    10081 caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca
    10141 taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga
    10201 gatgcgaaag ttcatccaag aagcagttga atcgcttatt cagcaaaatg cctatcttcc
    10261 cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga
    10321 ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc
    10381 agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca
    10441 tactgacgcc tcttattcaa aacgctactc tataaaaatc ctgaaagaag actttcggga
    10501 aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa ctaaagaaac ggtaaagaaa
    10561 gacgatttca atgaaaaata gcaagtaaac gataacaacc atcctaaatc cacaagaaat
    10621 acagcagacc ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag aagacttaac
    10681 gcgtacccaa tattagtcat ttgactatta ggtacgcgtt ttgttttata agtcatgaca
    10741 aatttccttc gttgctggat agattttccg gcattgttga tagattttat gatacttttc
    10801 tacattttgt aagagaggtt ccgctgcatc tgtatattgg ataaaattct gacaacaact
    10861 ttcaggtgtg tcgaaccacc ctaaccctag tgcagcaatc attgttgcgc ctaatgcagg
    10921 tccttcctcc atacttaaac aatagaccgt tgcatcgaaa atatcagctt ggatttgtag
    10981 ccatgcaaga tttttcgcgc ctcctcccac cgaaacaata cgtgtaaatc tgcgacccgc
    11041 tagttcttta cgtttcaatc ccaccattga caaatgctca ttaaaaattt agaatattca
    11101 caagtttaac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt tattggatga
    11161 aaagttagat ataaaaactg cgatcaaaga cacgttacct accgttttcg gttatatcgg
    11221 tattggactt gcatttggta tcgttgggaa agctgccgga tttcatccac tagtcgtcac
    11281 gttgatgtcc ctgctggtct atgctggttc tgcccaattt atcacagtca gcatgcttgc
    11341 tagtcacagc ccattgcttt ccatcgtttt ctcgaccttt ctagtcaatt cccgaatgat
    11401 tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc aaaatctgct
    11461 gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat tgaactatac
    11521 tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt atttgacgtg
    11581 ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc cgaaaaagtt
    11641 tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc aagtcatttc
    11701 tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt tgattctggt
    11761 atatgtcgga ctgattttta tcccaagcaa cttagtgatt gtagttgtaa cactgattgg
    11821 gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg ctgactattt
    11881 taggttgtac tttagcaact tggctttcta gggtcctacc atttgtctta ctgaaaaaat
    11941 tcgatttacc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc attatgtctg
    12001 ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag atcaatctcg
    12061 aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa agcttattgg
    12121 tgatcgtctt ggcaggaatt ctttcccttt cgttgattcg cttattataa ataaaaaaac
    12181 aagagcttat ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa
    12241 aaatcggcgt attcttgctt atccgttgaa cggaccacta acaacgactt ttcctaacat
    12301 atgtcctgtt tctacgtctt tggttgcttg tttcaagttc ttcgcattca tcccatctga
    12361 atagaccttg ctcaatgtac tgcggataat cccttcattt gctaaagcag caatgtgcgc
    12421 taaagcttct ccttgcgttt cgatttcata gttatagtct gttttagcga acatatactc
    12481 ccagtcgaag ctgatagatt ggtttttcca aacacctata tctaacggct ctttgatccc
    12541 tacgatcgtt cccacatgtc caaatggtcg gacaaggtgc ttgatttgat caaagtagcg
    12601 ggtaatatcg aataatacag cgacatactc tacttgatcg attcctaatt cacgaaaact
    12661 tttacttaag tcgtgatgat aatcgattgg atagtctaca cctgtttttt tcagccattc
    12721 aaaattcttc ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt
    12781 caaaatcgaa ccgacgcccc cggatccgtt gatcactaag atttttttcc cgcgattggc
    12841 attttcttct ggagttagac caaatttttc aaataacaat tcataagcgg tcaatgaggt
    12901 caacggcaag gctgcagctt cttcatctga aagatttttt ggtgcaagag cgacaatacg
    12961 ttcgtcaact agttggtatt cttgattgct tcctgctctt gttgttgtac ctgcgtaaaa
    13021 aacgcgatcg ccgacagaaa atttttgcac ttcgtcacct acagcaacaa ctttcccgac
    13081 tccatcaaaa cctaagatac gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt
    13141 atcgacagga ttaacagata ctgcgttgac ttttaccaga agatcccgtg aactgggtac
    13201 aggtatctgt tctttttcat caataaagct ttttggatca tcgatcggca acccttcata
    13261 aaaaccgact accttcatta attccttttc tgacatacga attcctcctt agcttcatca
    13321 agttggagca tccatacagt gttcatttca atatatatag tatgctattt gttccacctt
    13381 ttttattcat cataattatt ctcttactta aagtaaacta tttgcacttt tttggttaaa
    13441 aaagaggttg cgacaagact tttgtcacaa cctcttataa acggtgttct cctaccaact
    13501 cttcggtcac aagtcaaaat attcatcaaa catgagaagc tgttttttga aattgctcct
    13561 tgtgactcag agttaaacgg caggctcaca acctctgata taaacggtgt tcaatcaact
    13621 gaccctcggt attagctcaa aaaacgacaa aatatgaaga gctatttttg tttggctgtt
    13681 cttattactt gatacaggac agctttttca cgacctctaa ttcacggtgt tcaagcaaga
    13741 aatcattgtt cactgaaaag cgttggatgt tctttttcaa aggtctctaa gatctctcgt
    13801 acttcatcag tagatttcgt attcatcaac tgaactcgca agtcatttgc tcctggaaat
    13861 cctttgatat agattttaaa gaaacgatgt agtcctgtga tggaacgcgg gatgatttct
    13921 gaataatgat cttgcagatc tagttgcaga cgaagtagtc cgatcagttc tttttctgta
    13981 tgttccctcg ggtctttttc aaatgcataa ggattcttga aaatccctcg gccaatcatg
    14041 atgccatcaa cgccatattt ctcggccaat tcttctcctg tctggcgatc ggggatatct
    14101 ccattgatcg tgattagtgt ttgtggagcg atttggtcac gtaaagcaat gattttagga
    14161 atgacttccc aatgtgcatc tactttactc atctctttac gcgtacgcaa atggacagaa
    14221 agattggcta tatcttgatg aagtaggtga ctgatccatt cttccatctc agacatttcc
    14281 gtatagccga tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc tgcttcaatc
    14341 aattgtgccg ctacttctgg acgaaggatc aagccactgc cttttccacg ttctgctaca
    14401 ttagggactg ggcagcccat gttgatatcg attccttgga agcccatttc tgccatagca
    14461 atgctcattt ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac catcggctgt
    14521 tcatcttctg taaatgttaa tcttcctcgt acactgtctt ttccatctgg atgacagaaa
    14581 ctgtctgagt ttgtaaactc agtaaagaag acatccggtg ctgctgcatg ctttacgaca
    14641 tggcgaaata caacatctgt tacatcttcc attggtgcta aaacaaaaaa aggctttggc
    14701 agctctttcc aaaaattagt agtcatttat ttgttactct ccattctatc aagcagtgaa
    14761 gtcgcaatct ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag aagcaaagtg
    14821 aacagcttaa aagatgtttc tgattgagca aaaaaaagac tgtgacaaaa atcttatcac
    14881 agtccatttt ccggaataaa tggtgttcaa aagcttgcat ttgcggtaat aagtccaacc
    14941 aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga tgcagaacag
    15001 ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt attcgctcaa
    15061 aaaacaacaa aatatgagga actattttcc cgtttgtttc tctaatactc aggtcaaaac
    15121 gctgatttca caacctcttt tctaacttct caatcctctt cctttattga tcaaatacca
    15181 agtaacggcg tacaaaacaa cgatgaataa cacaagtacg atcatcgatg ttgctactgg
    15241 cacatcgatc gttccaagga atccgtaacg gaaaccggag atcatataaa cgatcggatt
    15301 gacttttgaa acagcttgcc aaaagggcgg taacatagag atggcataga aaacgccacc
    15361 tagataagtt aacggctgca agacaaatgt aggcacgatc gatacatcat cataagactg
    15421 agcaaaaatc ccgttgagca gtcctgctaa tgaaaacaag attgccgtca tcaataatgt
    15481 aatgatgacc atgaaccatg aatacacatg aagtggtaca aagaacaagg aaatgatcgt
    15541 tacgagagag ccgacgagaa tactgcgacc cacaccacca atcacaaagc cccaaataat
    15601 cacatgcgta ggaacaggtg ctaccaagat ttcttcgata tttttctgaa atttttgaga
    15661 gaagaaggac gaagaaacat tggcatacga actagtgata gcagacatca taatcaaacc
    15721 aggaacgatg aattccatat atgaaaaacc acccatgtca ccgatccgcc caccgatcat
    15781 ttttccgaaa ataacaaaat acaaggatgt cgtgatcact ggcggaacca gtgtttggac
    15841 ccatatacgc aaatagcggt tcgtttcctt gatagctaaa ctttttaatg cagtaaaata
    15901 aagattaaac atttttctcc tccgtatgat tattttcttc tgtgattttc aagaacagtt
    15961 cttctaaacg attcgactta ttcctcatag aaagaacttt gattccttgt cttgtaagct
    16021 gatcgaagat tccgttcacc ccttgatttc gttcaacttc gacagcgatt gtctgctcat
    16081 cttcaaatgt acaagcatat ccttcaatca caggtttggt atcatatggc gctaaatcaa
    16141 agataaatgt ttcaaattgc agttttgaca gaagctcttt catacttgta ttctcgatca
    16201 gttcacccga ttggatgatt ccgatgttgc gacaaagcat ttcagcttct tctagataat
    16261 gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc tcgtagaaac tcccacatct
    16321 cgcgtctcaa ctcgatgtca actcctgctg ttggttcatc caagatcagc aaacgtggtt
    16381 catgcatcaa tgccctagca atcatcagtc ttcgcttcat tcccccggaa agcatacgtg
    16441 cacggacatt tctcttttcc caaagattgg actgcttcaa atatttttcg ctgcgtttca
    16501 tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt cacgacgatt tgctggaccg
    16561 tttcaaaagg gttaaaattg aactcttgtg gaaccaatcc aatctgttgc tttgctcgaa
    16621 ctagatcctt gtccagatca taaccgaaga cattcacttt tccagatgtt ttattgacta
    16681 aagaagtaat aatcccgatc gttgtagact tcccagctcc atttggccct aatagcgcat
    16741 agaaatctcc ttctttcacc gaaagatcga ttccccgcaa tgcttcgacc ccacttccat
    16801 agatcttttt caaattattt atttcaagcg catttgccat ttatttccta tctccttata
    16861 gacatgattc ttaattgtat catggaatac agcagatgaa aaaacaactt ggcttccaca
    16921 caaaaacaca cctgctaaca aagaggagat gtgttttcta gttcatgatc atcttcgata
    16981 gaataactat ctaaacaact ctggaacttt taaaatcata aaagtaccga cagcaactac
    17041 tacgatgaca tatagaagat ccttataact gaggttcttg tcttttttat tcatgttaag
    17101 ctttcctccg tccttttatc gaaagctttt ttcttttatt ttaaccgaaa taaataaaat
    17161 ggaaatagtt attttcaatt tctgttttcg ctgtttgttt tacggagaac tttgccatgg
    17221 aacgtgcgtc tcgtcgctga tgctaagttt tttcgataaa agctgctaga aggaagacag
    17281 cgtggtttat tttgtatcgt cttttgctaa atcttccaag atcatctgga cagcagactc
    17341 gttcccattg aattctgcca cttttttggc atcaatgtac ttcttttgat aatcgtcttt
    17401 ttggacccac gcatcatgct tcgtttgata aagttaatca aacggatacc actgacgctg
    17461 ttcatcgaac aacaaacttt ctttttgggc ctgttcaact atggatttag gataattcaa
    17521 aactttcagc aaggcaatcg catttcgggt ccttgaaggt ccttctttta atttgaaatc
    17581 aaaactgatt ccttttcctt ctgttacttg ttcttcaaag tggacattct cacaataatt
    17641 ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag atggatactc
    17701 cgcaagccac cgaacgactg aagcggaaga agcaatccgt tcgatcgtat ttgttccttt
    17761 taaaatctca tctacgaagc agtagcagcg ttctttggaa gccacttggt ccagcaaacg
    17821 tttgatcgat ttgatttctg aaacaaaata actgtcgcct tcaaataaat catcttcgat
    17881 tgccatggaa gtcaacacat gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg
    17941 gatcgtctgt gctagaatac agctgatggc tatacttttg acatacgtag attttcctga
    18001 tgcattggaa cccgtgacta atgtattctt ctgccagttg actggattaa ctaccgcttc
    18061 ttttaacaat ggatgataga tatctttcgc tgtcacaccg ccctcagtaa actctggtcg
    18121 gcaagtgatc ggcatatagg tgcgataatt caaaacggct gctgcaactt ccaattctcc
    18181 cagcaattcc cacagcttga tggcttcagt tgaatgcttt tctaatttag tgagaacgaa
    18241 atgataggag ataaatggca gcatgaacat gatgttgaca tattcaaaaa tcaattctgc
    18301 ttcactcccg ctcttcattc gaaatgaaaa acctaattta gggatgtctc ttaatggttt
    18361 agtttgctgt ttcagctctt tttgaagtgg tgtcgtcatt tttgctaact gattggcggc
    18421 tgaaatcgtt gaaaccaaat aactcatgct attcagctct gtttctagtg tttgtttctt
    18481 cactagataa tagaccgtat tgaatactaa gccaccgatt gctagataga ttcctagtac
    18541 ttgccctaaa agaagcaaac ctacacccac cagcggaagc aagccaagaa aaataaataa
    18601 acctacgcgt ccaatctttt tactttttcc atccgctaga tattgttttg caaaattatg
    18661 atcttttttt cctaaacgag cgaattgata ttggattctt tctctcagtt gaggattttc
    18721 ctgataaaaa gcaattagtt tttctaactg ataatcttct ccaaaatcaa agctacgtaa
    18781 acgctgatac aacgcttctg aacctacact agaataggta ctgttgatcc cttcaaatac
    18841 ttcgaacata tccaagtcat accaagtaag atcgtcgatt tcactatccc atgaacggaa
    18901 ttttttctcc gtgagccacg catccttcaa actttcttct ttatcaaatc tcggttgata
    18961 aggaattttt ccccaattac tacgaactgt ttcctttact ttcaaacgtg aaaaaatgtc
    19021 taaaacaact aataaaataa caactgcaat aataattaaa acgataaatt ctctttggcc
    19081 catatccctc tccgttcctt gatttgttag ttttagtata gcactgttgc gctatttgaa
    19141 aagggggtac aaaaataact gtcttcgtta cctaattcgt atcaatcaca ccaaaataaa
    19201 aacaccttcc aaaaaagaag atgtctttaa aacgagatac aattttttgt cattcacttc
    19261 aggtaaatta gagccaatta aaccctccgc aacttgacgg agatggagaa gatgcccccg
    19321 tctaatctag cggtcatctc tcctcccatt ccttccacca atttttttga caggtaaagc
    19381 ccgattccgc tagattccgt atttgtgcgg ctggtatttt cggtataaaa acgctggaag
    19441 actttttcaa cagaggtttg cgctcctggt ttgataggat tggccactgt taatcgaatg
    19501 aatgaatcct cttttgaaaa tgtcaccttt gcttgtccat ctgcataacg caataaattc
    19561 tgtatgatat tttgcagcac tcgcagcaac atgagcggat ctgctaatac agttgtattt
    19621 atcgagtgtt cagcgaacgc tacctcgata tttttctcag aaaattgttc ataaaaagag
    19681 aaaaatactt cttccacctc tttagaaaaa gaaagcgtat ggagatccat ttctttttgc
    19741 ttcgtctcta ttcgagcaag atcataaaac tgatccgtca tactgagtaa tcttgcaaca
    19801 gattgtttga tgatcgttaa atacttctcc cgtttctccg tatcctctac ctgttccagt
    19861 aattgtacat aaccgttaat agcagtcaaa ggagttctga gatcatgaga aaggccgacg
    19921 atcgatagtc tgatgttttc ttccatttct tgaaatgctt gtcctttttg ttcattttca
    19981 tccacccatg tattcagtgc atctactagg tccattagat tcttttctcg aaattcgact
    20041 gaaagacgac caccatattc ttgtcgttca gaaagagtgg cgattttctt agttaatctc
    20101 ttgatttccc tttgcaggaa gaaaataaaa cttcccataa agataacaac gagaagcaac
    20161 agccacttaa tcaaatctaa accccactcc ccacaccgta tgaatataca cagcatgtgc
    20221 ctttgctagt ttccctcgaa gattggaaat atgcatattc actgttttct cactatcaaa
    20281 atatggttct tgccaaactg tttcataaag atttgctcgc gaaaatactt ttttcggatg
    20341 ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc aaatggacag ccacaccatt
    20401 cacatttaca tccctcgact ctttgtccag taagatttcc ttgtaataga tgcgttgcga
    20461 cagatctttt acttggacag aagtctgatg acgaagttgg atgccgattc gtaacaacag
    20521 ttcttcgata tcaaatggtt tcgtcacata gtcatccgct cctgctgcca acaaggaaac
    20581 tttatctctt tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg atgtctgtcg
    20641 gatttctgct aacaatgctt cacctgaaag ccctggaagc atgagatcta gaaggatcaa
    20701 atcgacagga tatgtgctta acaataattt tccttccgta cctgaataag catcaagaag
    20761 tttgtatctc tgttcaagaa cctcctccag taaagaatga acagcttcat cgtcttcgat
    20821 aatcaaaata gtttccagca ggaacaccta cttaatgtca gatttttgga aaataaacat
    20881 tcctagagct gatgatacca cgattgttac tagactgacc agaatcattt tcggtgcttg
    20941 cgaataagtt gcttgttgcg caaagtccgt atacatgagc cacgtttccg taaaccactc
    21001 cgttagaaat tccaagcctt tgatttttgc gaaaacaccg ggaacgaagg ccgttcccat
    21061 gatataaaga aaagaaagaa gcaaactgac actggtactt ctagtgatga atgtcaaagc
    21121 cactggaaca gataatgtcg ctaatatcag cagaagcaat acaggaaata cttttagaac
    21181 ataatttcca tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa gtgtgtaagc
    21241 aatcatggct agccccgtaa tcaaaaccac gccaagtgtt gtgatcaaaa gcgtaacgag
    21301 aagtttcgac acataataag tcattcgatt ccgtcctaaa gataacgtat tgcgaatcgt
    21361 accgttttga aattcttccc caatataaaa gcacgcaaat gctgcgaaag ccagaagaat
    21421 ccatgcagcc attttttcca ccattaaaat ccctgttgca ccgttgatga ctgtatccgt
    21481 tgcttggttt aattcattgc cgtattttgt atgcatatac cattcgttcc aagaataaaa
    21541 aagcggaagt acgaccatca gtcctagtga gacccacatt ttacggctct tccatacttt
    21601 tattttttca gcagttaata gattaaacat gtggattgcc tccagttaat tgtaagaaat
    21661 aatcttccag tttttgccca actaggtgga taccatccac aggaactttt gcctctacca
    21721 atgcttgatt gatggctgct acttggtcaa ttccttcaaa aagctggata acacggctag
    21781 attggacaaa ataatcccta taaccaagag agtcgagaac cgttactgct tgttccgtcg
    21841 ccgaagtgtc aatttttata aattgacgag attcttgtgc caattcttct ttcgataatt
    21901 ctttgatcaa gtgtccttca tgcaaaatac cgtagtgagt ggcgatctgt gacaattcat
    21961 ctagcaagtg gctagaaagc aagacggtga tgccccgttc ttttaccaaa cgttggatga
    22021 tttctcgcat ttctgccatc cctgctggat ccatcccatt cgtcggttca tccaaaatca
    22081 aaaattctgg attcgtgatc agtgcatttg caatagctaa acgctggcgc atacctaaag
    22141 agaaattctt tgctttcttt ttccctgtat tttccaatcg catcattttc agcaggtctt
    22201 cgatttctct atccgacaca ccgccgttcg ctgcttggat acgtaggtta tctcttgcag
    22261 ttaattctgg ataaagagca ggggtttcaa tcgattgacc catacgtcgt cttgctgcct
    22321 gcaaatccgt cgttccaaat aattcgatcg aaccttcatc aatcgagatc aaacccataa
    22381 tagttctcat aaaggtagac ttacccgctc cattctcccc aatcaaacca tagatcattc
    22441 cacgtttgat ttcgatcgat actttatcta atgctttggc agcaccgtat tttttggtta
    22501 taccagttgc tttcaagacc gtttcatttc caaaatccat acttatcctt ctttcttatt
    22561 gataagtgaa gtatagttga cgggtataaa gaaagcagcc agaaatgtat aaagaaagtg
    22621 taaattttcc aattgaattt caagaaaaat cagactgtct cgcagacgtg gttgagttgt
    22681 ctgccaatca aagagcgcac caaacagtgc taatccaagt ggactggcca ccatggtaat
    22741 cgttgttatc gttgacatca gacgtccttg tatatgcgtt ggaacttctc gctgaagcca
    22801 gaccatataa aaagcaaaag aaaacattaa tccttaaaaa cggacttatg aaaaataata
    22861 catgccgttt gggatttaca attagctacc aaataagagg ttatgacaaa agacttttca
    22921 caatcccttc ttccgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
    22981 atttatcaaa catgagaagc aatttttgta aattccttct tatttcttga agctgaccac
    23041 ttctgtcaca acctcagaag cggataaata atattgtttg atgatacttt atctttttcc
    23101 cgtttgttta tagatcgaat gatattatag accaagaatc ctacctatta acatagctaa
    23161 aaacacaaaa aaggcacttt ccaaattgtg gaaagtgcca gaaaagttag gattaaggta
    23221 actgcgctca gcattgcttc gcccagtacg attctcgtaa ctcgtgaact cgaacgataa
    23281 tcgcaattaa cgttttgaga actgtgaagc tttacgggct ttcttaagac ctggtttttt
    23341 ctttatatca ttgaatagcc ttgaaaaacc ttgatataaa aaggttttag taaaaatgat
    23401 tttatcaaat ttgaataact ttgaaatatt tgtgttctaa aatgtgttct aatttaaagg
    23461 tagtatattt
//
LOCUS       DAXWLG010000028        23295 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXWLG010000028 DAXWLG010000000
VERSION     DAXWLG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.028"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            125..346
                     /gene="relB"
                     /locus_tag="ACGE4T_000861"
     CDS             125..346
                     /gene="relB"
                     /locus_tag="ACGE4T_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8386163.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            348..614
                     /locus_tag="ACGE4T_000862"
     CDS             348..614
                     /locus_tag="ACGE4T_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8386164.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            781..1266
                     /locus_tag="ACGE4T_000863"
     CDS             781..1266
                     /locus_tag="ACGE4T_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386165.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVGTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKLQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     T"
     gene            1475..1657
                     /locus_tag="ACGE4T_000864"
     CDS             1475..1657
                     /locus_tag="ACGE4T_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8386166.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            1760..2044
                     /locus_tag="ACGE4T_000865"
     CDS             1760..2044
                     /locus_tag="ACGE4T_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386167.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            2060..2296
                     /locus_tag="ACGE4T_000866"
     CDS             2060..2296
                     /locus_tag="ACGE4T_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386168.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            2318..2572
                     /locus_tag="ACGE4T_000867"
     CDS             2318..2572
                     /locus_tag="ACGE4T_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386169.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            2654..2839
                     /locus_tag="ACGE4T_000868"
     CDS             2654..2839
                     /locus_tag="ACGE4T_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFF8386170.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            2906..3301
                     /locus_tag="ACGE4T_000869"
     CDS             2906..3301
                     /locus_tag="ACGE4T_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8386171.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            3318..3668
                     /locus_tag="ACGE4T_000870"
     CDS             3318..3668
                     /locus_tag="ACGE4T_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386172.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            3690..4064
                     /locus_tag="ACGE4T_000871"
     CDS             3690..4064
                     /locus_tag="ACGE4T_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386173.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(4371..4769)
                     /locus_tag="ACGE4T_000872"
     CDS             complement(4371..4769)
                     /locus_tag="ACGE4T_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8386174.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(4961..5728)
                     /locus_tag="ACGE4T_000873"
     CDS             complement(4961..5728)
                     /locus_tag="ACGE4T_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8386175.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(5763..6923)
                     /locus_tag="ACGE4T_000874"
     CDS             complement(5763..6923)
                     /locus_tag="ACGE4T_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8386176.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLENHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            7967..8770
                     /locus_tag="ACGE4T_000875"
     CDS             7967..8770
                     /locus_tag="ACGE4T_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8386177.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            9355..9810
                     /locus_tag="ACGE4T_000876"
     CDS             9355..9810
                     /locus_tag="ACGE4T_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386178.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            9929..10987
                     /locus_tag="ACGE4T_000877"
     CDS             9929..10987
                     /locus_tag="ACGE4T_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFF8386179.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            11029..12978
                     /locus_tag="ACGE4T_000878"
     CDS             11029..12978
                     /locus_tag="ACGE4T_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8386180.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            12981..16622
                     /locus_tag="ACGE4T_000879"
     CDS             12981..16622
                     /locus_tag="ACGE4T_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFF8386181.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            17022..17360
                     /locus_tag="ACGE4T_000880"
     CDS             17022..17360
                     /locus_tag="ACGE4T_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386182.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            17470..17691
                     /locus_tag="ACGE4T_000881"
     CDS             17470..17691
                     /locus_tag="ACGE4T_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386183.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            17711..19867
                     /locus_tag="ACGE4T_000882"
     CDS             17711..19867
                     /locus_tag="ACGE4T_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386184.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            19879..20112
                     /locus_tag="ACGE4T_000883"
     CDS             19879..20112
                     /locus_tag="ACGE4T_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8386185.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            20102..20968
                     /locus_tag="ACGE4T_000884"
     CDS             20102..20968
                     /locus_tag="ACGE4T_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386186.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            20983..22953
                     /locus_tag="ACGE4T_000885"
     CDS             20983..22953
                     /locus_tag="ACGE4T_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386187.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         8420 a         3462 c         4382 g         7031 t
ORIGIN      
        1 tttatattct acaacataaa catatatatg tatatgttta tgtttatgta tatattcaaa
       61 tatataacta ttgcatttgt catacaatcg tagtacaatg taattgaaaa ggagttgata
      121 aactatgcca gtaataagta ttcgtgtatc taaagaagaa aaagattttc ttgaaaaagt
      181 tgctgaattc aacggagata acatttctga atttatgcgt agtaattccc tgcaaacagc
      241 agaaacgtta gtagactttg ccacctacaa gcaactaatg aaagagcatg aggaaaacga
      301 tcaaagtatt tcccacgaag atatgctaaa agagttaggc ttataagatg acttaccaag
      361 taagatatga acgtgaagct caaaaagcgc taaaaaaaat ggataagttt caggcaaagg
      421 ttatcctaaa ctggatcgag aaaaatcttg taggcactga tgatccaaga aagcatggta
      481 aaggtctaac agctaataaa tcaggctact ggcgatatag ggtaggtgca taccgtataa
      541 tagctgatat tagcgagcat gaagtgacaa ttcttatatt gtctattggt cacagaagtg
      601 aagtttataa gtaacagaca agctgataag gcttgtctgt ttttttgtta acccaacaaa
      661 atcactgttt acgatcccac tttacctaaa aaagaacaat atcctcttga tattatacac
      721 accagtgtgt ataatataag tgtaaacaag ctaattaact ttcaaaagga gcgatttttt
      781 atgggatggt atggtgatta ttcatcagca aatgaagtga tcgaagaaaa gaaagggtac
      841 gccaaaaatg gtttggaaat cgtaggtacc aaagtaacga agacctatgg aattatcctt
      901 ttcaaagatg cagaaggaac ctacgatatt gacagcttca tctttcgaga tgaaatgtac
      961 aaaccaatct acttcttaaa tgacagtcct gcttacttta agaaaattcc gaaaaaatgg
     1021 ttgaaattct ttgtcggtga tcaagaaaaa atggttaagc tacaactaga tagaattaaa
     1081 gaagaaagca ataaaccaaa acttgatgac ttattagttg caggtaaaac ttactcaatc
     1141 tgggatggat cgtatgtcgc tgtctatcag tataaactca aacgtagctt tatttttcga
     1201 ctacaagatg gatcactcac tcggttcact cgactatcag caggagatgt taaggaagta
     1261 acctaaaact taaatacata aacataaacg tgtatacgtt tatgtttatg tatatgtata
     1321 tgaatataaa cgtattaggt acgaacactt ttaaattctt ttctgggctt tgtttttagg
     1381 gtagggctaa aaaaaacatc accatctgat ccaaaacagc tcagaaacgc atttaaacgt
     1441 atctgatacc aatattacag aaggagtgta tcgtatgatt aacctatttc ttttagtgac
     1501 agctagtaca ttattcttta ctggaatatc actgataatt tcctatttga tctataagaa
     1561 agccgaaaat cacacaagca aagcaatcgg actagccgtt tcatcctttt taactttgct
     1621 gactttattt gttactttat cgttttcttt tgtttagaac agctgatcca ttagcacctt
     1681 tttctcttta cattatacac actgttgtgt ataatgtatt tgtaaacaag ctaattaact
     1741 tttaggaagg agtgacaaga tgctaacagc tactttacag caacctactc acgttgctat
     1801 tgcagaaagt tatacaatta atgcagggat cgttaaacag ctgatcaagg atcgagaatt
     1861 caaaaagttt aacagtcggc aagcgtataa cagacagcta aagaaagcta ttctaaagca
     1921 caaagattcc ttcatcggga acttggaaag tgtagatgtt aaaactgttt caacggcatt
     1981 ttcagttgtt gaagtgacta ttgacggtcg cttgatttct ctatttgctt ttggaattaa
     2041 ttgataggga gtgtgaactt tggaaggtaa gacttttgca gaaatttgga ctgaatttat
     2101 tgaggtacca actcgcattg atccgataac ttctgaaaag gtattggatg cagacttcta
     2161 caatctcaaa gcaggtacta gccttgaatg cgtttggatg tatttagaag agcatttcaa
     2221 aggcgtaacg ctcggtcacg tcttatacca caatggtgcc gagtgggatg caattggaaa
     2281 tatccatttt tcttaatata agaaaggaga acacaatatg aattataccg atgaagaaca
     2341 aatcgctttg gaatacgttt tcacaaatga agaaggggag gaaattgaaa aagtcttgat
     2401 catttcatca aaagacttgg agaaggcttt ggcttatgaa ccatacgaat caaccgtaac
     2461 tgtcattgaa ttcttagata cctatacttc taatgatact tgggaaatta tgaatatttg
     2521 caacgctaat aacattcaat ttgctgaaaa aaccaataaa tcattttatt agttttgcaa
     2581 taaaacatcc tctttttcta ttgattccat acacaacaat gtgtataata gattttgtaa
     2641 agggagggta gctatgccta agacagcaag agaaattgta aaaattctca aatctaacgg
     2701 cttcaaaaag actggacaaa ctggcagtca tgcaaaattt tataattcca agactaaaag
     2761 aacggtcgtt gttccgatcc acaatggtga tgtaccacta ggaacagaag ctaatatctg
     2821 gaagcaagca gggctttaac agccttgcct tcctatttgg tattaggcat actatcctct
     2881 cttacactag aaaaggagcg aggaaatgaa gacaaaaaat attttttatc cggcaatctt
     2941 cgagaaagaa ggagaagctt ataacatcgt tttccctgac ttaccggaaa tatctacctt
     3001 tggaaatagt cttgaagaag catatttaaa cgctaaggat gctttaggat tagcgctata
     3061 tagtgtacca gatgcagaat ttcctaaacc atcagcagtg gaagaattaa ctttgaaagc
     3121 caaccaagtg gtagtaatca tccaactaaa cattaaactt ttccgaagaa gcttaaatac
     3181 aaaaacaatt agaaaaaatg ttagtgttcc ggaatggtta gttcttttag ggaaggagaa
     3241 aaacataaac ttctctcaac tacttcaaaa agcactagaa gaagagttgc ttgaaaagta
     3301 aaaaggagac cgaacctatg aaagctacac acttaaacta tttctctcga acgttaatca
     3361 ataatctatt aaaagataca actggcatta gcgttagtac caaacacttt gccaatgatc
     3421 taattatttt tgaggataat aacttgcgaa ttgagatgtt tattgaacag gcaaccccta
     3481 actcaatacc tgaattctgc tatacgcaca ctgaaaaaat gaaacatgca aaatcagtta
     3541 aagatgttga agcattaagt gaatcactaa cgaacgcttt aaacctcgtt aagaaggcaa
     3601 aattgttatt tgaatttcaa accgactacc acaatgagtt gcttgctcaa tctgaaattt
     3661 tagattaatt gaaacaggag aagagaaaga tgaaacaaac acaaacagaa accgttaacg
     3721 atacgcttgc tgatcaatta gcaggttatt ataaattatt tgagatgggt aaagcacagc
     3781 gcttgatcca ctatctttgg aaatacaaaa ttttgtttct tttttgggaa tcaacactaa
     3841 cattctcagt tcttttttat aagtcagatt tttataatca acacttagtt ttagggagtt
     3901 ttttgcttat ctacaccatt ttgatgctta atttatatac ctacatcaca cttgaagaac
     3961 acttagtaat tagcgatgca gggatcgaga gaaacatcaa aagggtatcc ttcaagatca
     4021 cgttaaacaa gcctaccgat aaaaagctct cagaagcgtt ctgagagctt ttgacatttt
     4081 cttgtataat tttatcctaa cacattacaa agcacctaat tcgagcttat atcaagcaaa
     4141 tttgctattt attactcaat acatatctaa aaattacttg atggaaaatt agatacatgg
     4201 taagcatgaa aagaaaatca atttgagcag atgcagtaca atacatagca atttaaaagc
     4261 tctcagaagg cttctgagag cttttaatat attctttgat aatttgtctt tcttaaatcc
     4321 atacgtccta aaacaagctg tttttgctta atatgatcat ctgagagaca tcaaatcaac
     4381 ttttctctca acaaatcatt caaagctttc gacagattaa tattctcttc agccgctaaa
     4441 acatttaaat agtgttccac agtcactgat tttcgatcat aattttgctt gtttcgagct
     4501 ttagcaactt tcgtattagc ttttacgaca aataaattaa cattctcaga tcgcttgatt
     4561 cttgcattta actcttgaac aggcgtaggc gatggaattt ctttttcttg caactctaaa
     4621 agcaccaagt atccttccaa tacttcttgc gccatctcaa aagcatcttc taaagtttca
     4681 ccatgtgtat ataagtctgg aaaatctggg aatttaactg catattcatt tcctttccat
     4741 tcgaatactg cacaaaaaaa gtatttcatc gcattgcacc tctctaatta aaaaataatt
     4801 tttgcttttt tgtttcaaaa aaaagaatcg cctaccaaaa aaattgatag gcgatcacaa
     4861 gctaattaac ctctaaagga gattaatacc ttgctcccaa ggcactatat atcttagcat
     4921 acgtctgaag catcttcaaa cgagtaagat atattttgca ttaacctaat tttttttctc
     4981 tataagtttt ttcatcaaac tcactagaaa tattaaactg gacagcaaaa ttgtaatttc
     5041 tgccttttct aactttagca aaatcaatat tcagcattga tttttcatta atttcctcaa
     5101 ctgattccct taaaacacgt ctatcaaaat tcgatattgt tttatactca ttttctgttt
     5161 tcgtaatctc acgaatttta actatttcaa tcaggggatt cttatatatt tcagctttct
     5221 caaaatctcc tttatttttg gcgataagat attttttata atacatcgcc aaatatttgt
     5281 ataacacgat gctatatttt ttttgaaaag atgctaagtc ttcaaaaaca aagaacggat
     5341 acttcttttt gattgagaca attaagggaa ttacatcttc tgaaaaactg aaagatacac
     5401 tatgctcttc taaatcccaa tgaatccgat caatagcaga tacagtagta tacgtcttat
     5461 attttttgtc tagattcgaa atttcataaa ctgcttgtct ttgcaaagtg ttaactatgg
     5521 tttttagttc cgtttcaaga ttttgatcaa catccatgta attaataatc gctttaacag
     5581 gtaaatctac tacgcctgtt ttctttagct ttgtgggtga gatataactg aacagcaatt
     5641 gataaagttc atacgacttt aaatccattt tcatgtcaat tgttgctaag aactcctcat
     5701 tgtactttgt cagttcatct tttttcaaaa gtttttcctt ccttcacgca ttttttcagt
     5761 tgttaatcat aaaggttcac ataaggcaaa taactcgtaa ttgatttttc gagatattta
     5821 acagtatttt ttattgtgta atcctcctta tgagtagcaa cgtaagatag gtgattgaca
     5881 actgattcaa atcctctatc tttggataaa cgatcataaa gcggataaac tttattctga
     5941 agtcggctca taagtttaag atcgcataag tcggcagaac tgatcaaaaa gtttttgatt
     6001 agtaatttag tgtatttgct attcatagct ttcaagtacg attgattcat tttctctgtt
     6061 ttatcttctg cgccttttaa ataagcaggg tctctttgtt cttgcttata aggtaccgga
     6121 actgacatgg tacgtgtaag caagaacttt atgtttgtat aagtatgacc cttcttgata
     6181 agctcatagt caattttaaa atcagtgtat ttattcagtt gggcaatcgg ttctttaatt
     6241 acacgtcttt caaagtctcg aaaagcttta tattttttct caatactaaa tttctttcgt
     6301 aaatctgcaa tggagaaaac aggatttttt aatctatcaa gtgattttgt actccatgtt
     6361 aagttatctc tatttgcttc atacagatta aaatctttag tgaataattg aaaaaaactg
     6421 atcgcatact tgcttgtcaa gttaactact tgatcaaatg tatattgcgt aaaatgattt
     6481 tctaagttcg ttaataaagg cataattgaa ctactaaatt taatagatac tgcatcagaa
     6541 aaatcattga attcagtaat ttctagcggt gagatgactt tgtattcagt cttaccgtta
     6601 ggtttagttc tttgaatttt aatgacagat tgctgatgca atttttcaat aacttgtttt
     6661 aatcttgaat gttttgaaga agatgaacta gaagacatca acgcaaataa agccttttta
     6721 gatactttga cggtattatt aaaaggcgga tttttagggt caagatgtcc tatagcgtaa
     6781 agataaattt tttgtggcat agcatccatt tcaacaatgc tatctacaag ttgattcgat
     6841 aaagtaacag ttaaatcaaa cttttttaga ttgctttcgg tagtttcaaa tttcgggttt
     6901 acattctttt ctgttatttt catacgattg cctcttcttt ctaagcttgg tacgagctta
     6961 aaaacttgga actggtacga ttccattgaa taagtataga cctgttttcg atacgttaca
     7021 ataagaaatg tatcgtaaat ttataacata actcaatgtc agtaaaacac attttatttt
     7081 ttccaatcaa atttcattga aaatacagca gaaatcgcta ttttagaaca ttttcaaaat
     7141 aaaaatcctt ttcaataaaa tacgaaatta taaaaattac caaaattaaa aaacaaatat
     7201 ttttctagtt tttagaaata aattcacata ctttttcttt ttttggatat attcggaata
     7261 ccacatcgct ttttgtccac gtttgcatcg ctttttgtcc acattcacat cgctttttgt
     7321 ccatgttcgc atcgctttat gtccacccta aaatctattt aagattgata taaagccttt
     7381 ttggaggttg ctaaagtttt aaagattaaa atatatatta taaatataaa taaaagggga
     7441 agctgaagct tcctcctttt cctttttttc aattattcaa agttacacaa gcaaaataaa
     7501 ctcctagata gtgttagttt tttttaagat tttttatttt taagattttg aaataagaga
     7561 ggagtaatcc tcctttatta aatatctgtt aatatatctg ttaaatatct gttaatatat
     7621 ctgttagggg aggcttaatc tcttgggaga gtaagtatta aaggggtata aaggtaacac
     7681 ttttggacag taaaggtaac aaaagtggac agtaagggta acagttttgg acagcaaagg
     7741 taacaaaagt ggacatttga ggtatcacac taggataaga cgttaccttc ggttttcttg
     7801 atttggaagg cgaaaaaata gaaaattaga ttagtaacaa atctttgttg actgttacct
     7861 ttagttgtga tttaatttag gtaacaagca gaaaaaattg tttcctttaa ttagtaccag
     7921 attaacatga ttaaaggagc gcaaaaagaa gattggagag taatcaatgt ctcaagaagt
     7981 taccgaaaag attattgata gtgatgtaga tttagtatca gaacaaacaa atgcaattgt
     8041 gcgttataac aatggtctga atacaacagc tttgcgagga tttaagcctg ttgaaatgga
     8101 tgtgttttgg gctattttat caaaaatgaa acgtcaagga acagcagaag taacttttga
     8161 tttcaacctc tttcgttatt tggtcaaata cgacagaaga gaaaaggaca gtttttattc
     8221 tgtgttaaaa gatttgagtg acaaactagg taatttaacc taccgttttg aagattcaga
     8281 cgtctatgaa cagctgtggc tttttcaaaa attccggata gataaacaga aggaaacagt
     8341 tacaattaag gcaaatgagg aatttgaatt tatcttaaac tcaattggca aaaactttac
     8401 tcgttttgaa cttgataatt ttacaaaact aagaagtagc tacacaaaag aattgtatcg
     8461 tcatctcatg caatacagaa ataaagagtg gcgaaatggt tattgggtag tcaaagttga
     8521 agattttaga agagcattaa gtataccttc aagttataga atgacaaata ttgataaaag
     8581 aatactagag caggcgaaag aagaattttt agctcctgat gaaaacggaa tacaggtatt
     8641 tgaaactttt gattatgaaa aaatctatgc aaagaaagga agaagagttg atcggctcag
     8701 attcactttt agtgagccag aatcaaactt gccaaccatt tcaatgcata attggttaga
     8761 agaagattga gaagaagcag gttgtaattt taatacaacc tgcttcttta aaaaagtatg
     8821 ggtaacactt ttggacaata aatgcaagaa gtttttttgt aagcaagttt cagtctaaat
     8881 aatatttgaa agaatggcta atctatacaa attttaacaa ctatgggtaa cacttttgga
     8941 caagtcaaga aaaaaaacaa tttttcttaa tttgttgatc gactaaaggt aacacttttg
     9001 gacaacacag aataaaaagt ccggtgatat cagttttggt aggaaacatc ttaatatctt
     9061 tgtgagattg aaggtaacat ttttggacaa gtcaactatt tgttaaaagt taatatagag
     9121 taattagata agttttttct tttcgtctga taaatggata atattagtta ttttattgct
     9181 aattagtctt tcatgatgac tgaaatagct aaaataaata tctcgttgga taattgctga
     9241 tactttaagg gatatctaaa tttgctgatt gatttattta tgttattatg atattataat
     9301 aacataaatg tgttacgccc gaacctcatt ttaaaaaaat gaaggggtga gacaatgaat
     9361 gctattaatt ggcatgaatt tgttaactat atggagagct tactagatga tctaaaaagt
     9421 tttagtattg ctgatgcagt aaagcatggg ttaccagtta gtacaacgat gaaattacga
     9481 ggattgaaaa ttccagatga aattattaag ctgagaacta aaacaattat tgaaaccgca
     9541 aaagtcgttg acaatatgaa ccatgaacta aaaggcaaga aaacaactta cgagaaagat
     9601 tttgaacgtt ttttaaaatc tgttattgga aaatatctag gtaaaaaatc tttcagtgaa
     9661 ttgagagatg aagagattgt acctactcca atggttagca aaatccgaaa aaacaaagcc
     9721 gaggggaatt actatcttgg tacaatagca aaaatcattg attatttgga aaataaagag
     9781 caaaagaaag gaacgaagaa aggaagataa tttttgtgtt aaatattaat ggggaagaat
     9841 ataccgaaat gattcaaaat cccgaaccta gaattaccga aaaaacgtct gattgtttgc
     9901 tcttttatga aagggggcag acaaactaat ggaaaaaaac gtcaattcaa catctaccaa
     9961 agatcaatac agtattattg gagggttgct tggtgataag gataagagaa cacgtcacgg
    10021 attacgcatt atgcttgcaa cttttctagg agtgcttttt ttattaacag gcttgggtag
    10081 ctacatttat caattaggaa actataatta ttatctcgtg aacaatacaa cacctatagg
    10141 ctcttcattg gagtttgctc gatcaggagc agagatcact atttcagatg tgtggacaga
    10201 tcaaaacaga gatgtaacag tagtaaaact cggttactcg caaaacgcta gaaagttgtt
    10261 atctacgcaa ggaaaaaact acaacttata tatggcaacg aaagaaaaag atcgtcctaa
    10321 aaagcttgaa atgagctatg ggatgttaag tactgatggg gatgctttct tattcctaaa
    10381 aggtaaatta gatgaaagag cttatcaagt ttttattgca aaccaagtat cactaatttc
    10441 tggtgatgat acagctgacg atgataccaa agtcaatgtg aatgaagcag attcagttac
    10501 aaaagcactt tctcaatatt ctatgaatga tgttaataat gaaggagtaa tttcttttgg
    10561 gaaaaaagca aaacctgtta atactcctga taatatcaat ttcagaataa atccatattc
    10621 agattcaact aatgtctatc aaggaagctt tcttaccaaa gatggcgata ttgattattc
    10681 aaaagttgta gcagttacaa gtaagaataa tgttgtaagt aagttagaag atcagttaaa
    10741 tgcttcaaat actaacatca agaaaattga agctacaaaa gctgagtatc tggaacgttt
    10801 acaacaaaat cctgacgata aagattcgca agaaagtatt cgtcaactag acagtcgaat
    10861 ggagaccgaa attaaagcaa tcaatgatac acaaaaattg attgatcaat acaaaaatgc
    10921 tgactttaac aaagattctt ttggagacat gaagacgaca tttaagtacc taactctgac
    10981 tgactagaag taaattattc ttattttaga ggaagtgaac acaaacagat ggaaaatagt
    11041 accgagtttt ttaagaatat tcaaactgaa tttaatcatg ctctcacacc aatgaaacca
    11101 ccgaaacata agttagtggg acgtgatgac gaactccatg agttgtcact gatcttgaat
    11161 agaccggaaa caccgcaagc tatcttgtta ggtgaagccg gaacaggtaa aacagcactt
    11221 gcaagtgaat gggctgaaag acagcgacaa gatggaaaga aagtacaagt atacaaatta
    11281 tcaatcggac aattgcgtgt tgggggagat gatgtcctcc aaacacgttt agaaacatta
    11341 attccaagat tgaaagaata tgagcgaatt ttaaaatctg aggacaaaga aaatgaattt
    11401 gtcctcttca ttgacgagtt tcataaagtt gttagctctt ttggtagtgg caataaaatt
    11461 gggggtgatt cgctaaaaga accgttagca aatgaatcac ttcaattgat taccgctact
    11521 acaagaaaag aatatgaaaa ctatatcaaa agagatgatg ccttagggag acggttacaa
    11581 ccgttagaat tgaatgaact aaaaagcgat gttgtcgtcg atattttgag acaatggcta
    11641 aatacatcct ctgttaaaga tgatcctcag gcttctttta aattatcgca aaggattcct
    11701 gatgaattac ttcaagaaat cgtacaagca aacaagcttt atcgtgagga tttgtccgaa
    11761 ccggctaaat caattgccgt aatggaggat ttgattgcaa aagatcgagt agacggaaat
    11821 gcttataatc gagaaagtct attgcaaata tttggtaagt ataatattca gctagattct
    11881 gattttgata tggaaaaact tcaagaggaa ttaaagtcca taaaagggca accgttagca
    11941 gtttatacta taacaatgtt gtttaaacgt atgaagcttg gatttgatcg ttttaaaggt
    12001 cgaaagcaac caaaagcaac tgctctgttt acaggatcaa caggggtcgg taaaactgca
    12061 atggctaaag ccgtggcaag aggactttat ggcgatgaaa ataaagttct cttagttaat
    12121 atgccggact acaaaacaaa agatgatgca atgcgtttta gacgactcat aggtactcat
    12181 gtaaggcatg atccttctgc gattattgtg tgtgatgaat atgagaaagc tggtgtagaa
    12241 acactcgatg cctttttatc aattttagat gaaggtagga taagttttac tgaaagagga
    12301 tgggatgatc gagaagttca cgacactgtt tcattgtcaa atacaatttt atttgcgaca
    12361 acaaatgcag gtgcggagat gttcgattcg ttaaacagat atagctcact atctcgtgtt
    12421 acaaacaagg aaaattctaa tgaatatagc actgaactaa aaagagaata ccgaaaagtc
    12481 gcatcaggga tcaaacaagc cttgattggt gaaggtctaa agcctgaagt tattaaccga
    12541 tttggacgaa tagttccatt ttgggcttta tccgaacaaa ctcttctgga aattgcttat
    12601 aatgaaatgt tgaagagtgc tgaattagta cgaaaacatt taaatataga tttacgtctt
    12661 ccacctagga aagaaagagt atatggtcaa aatttttatg cagacgatct ctccatgcac
    12721 ttagtttttg acatggcttc acaagaggat tctaaatcag ggggtgcaag gaatatcttg
    12781 catttagttg aatctgagtt tgaagaagcg attactgaag caataattac ttatccaaac
    12841 gcaaaaatat tcgatgttaa gttgagtgaa gccggagcgt ttgaaggaaa aggtgattct
    12901 aatgctgaaa ttattgcaaa gccttccgtt gctgaaagta aagaaaacaa gattgtattt
    12961 tcgattggag gggcgtagct atgaaattca agaaatattt actacaaaac cttagaataa
    13021 aacgatgggt cgtaagaggt ctagttattg gaggagtatt agcagttact ttaggcgctc
    13081 cttttggaat gaatgatcca actcctgaca atcaagatag caaacaagaa acagttataa
    13141 ataagaacgt tgcatatgcg gaaagtaaac cggcagaaca aggaatgtgg acactatatg
    13201 ctcgtatcct aatgggagat aaagaagtta atgatagtgc cgctaaaact ggacaaatga
    13261 atttgttagg acaaggtggt atcagtgccg atttccctta ttctgacatt tctaaacaag
    13321 gtaattcgat tgcagggggt aatgcaggta ctcaactagc atctacttta gcaacttata
    13381 gttactacgg atatattgaa acagtctcag gaaatgctat tgccagtcaa gcatcatcca
    13441 ttctaggaag tattggtagg acgattggta gttttatcgg atcaattagt ttaatccttt
    13501 ctaatattgc tgaatccata aatgaactcc ttggaaaatt tttcatgagt atgaatatat
    13561 tttctcttat ggggtgggga gaaggtggat cggatgtatc taatccggta tctaaagcat
    13621 taactagctt gcttaacaat ttgggattaa ataatgaatt atttaaagcg atagcaagct
    13681 tatctatgct ctttttcaca gcaatactag gttatcaagg aatgaaatta ttgtcgaaag
    13741 atggtgttaa ctcgaaggga ttatcaccag tacttgaaaa atggattgtt cgtatttttg
    13801 tattgtttat attcttacca gtggcaggat cgctattggg agtttatatc aacggcatcc
    13861 agtcagcaaa aaaagatgga gatataccaa caaatgttgt ttctgactac atcattaaca
    13921 caagactttg ggcttctact caaaatcttg ctccatcatc tgatactggt ggtgatgtac
    13981 ctgacggaaa gcctacatcc gaaatcaagt tcgttgatac agattatgaa ccatctaaaa
    14041 aatctgcaat gataagagcg atcaatacta gcagttatgc cagacaaaat aatgatctag
    14101 attctaattc agtcggtatg gctctattaa aagaatggtc atcgaatgat actttcaata
    14161 ttaatactta tgccggtgat gttaatgtcg ccgaaaatga tgcttatatg ggaagagcgc
    14221 ctgctgtttg gacaggctat aatttagggg atggaaaaga aggaagtgca attaagaaac
    14281 caaccaattt aaatggatat atttggtcag caaatcaaaa tgtgacagac gacaatcgca
    14341 agcctgatgg aaaaggaaca gcgcataact ttgatgctac tcaaccttgg ggcgttgcta
    14401 acaatgcaac ttttagcaca cagtcggtag ctttattgct ccaatcggca cttgatacat
    14461 cgggagctaa gttttatgcg tataatatcg ctccttcagg aattcaaggc agtgctaaga
    14521 atatgtcaac agtaaaaaca gaatggcgta cagcatcctt agttggtcat ggagctatgg
    14581 gtaagtttgg ctcgtggcta gcgatgaacg ctgaatcaat cgttttagca gttctttact
    14641 tagcatgtgc ttgggcgatg ttcaatgtga atatcgtgaa agcatttaaa cgtgaattta
    14701 ttcggatttg ggaagtagtt ctaacaggat cacttgcatc aggagcaggt gtgatggcaa
    14761 tttcattagg tgttgttaca acatctctga tcggactaca attacctatc tttctgatta
    14821 cttttattcg tgcatttggt gatactgctg tgggattcat tcctaataca ttagttggcg
    14881 atggcgtagc aaatattgtt cgatctgcta tgattttagg tgtaggatat cttttagtct
    14941 ttaaaaaagg cgaaaaagta aataatttga ttaatatgct tattggctta ccacttgaag
    15001 tagcgtttgg gtttgatgaa cgtgctaagt tattatttgg taatagaaat gatgctgtgc
    15061 gagttgtgga cggcgtttct aaagcaatca agaaaagcca aggagctaaa aatccctatt
    15121 atgatggtgg caacagctca gtaaaagata tggttgcagg aggtcttgga gcagttggag
    15181 cgcaaaaaat ggctgaacgt ttgaacaata tgggagatag caatgctgat gctaagtcca
    15241 ttgctgataa agtaattcct gaaacaccag aaggaatgtc attcaacggt ggcggagttg
    15301 gttccacaat caataatacc tctttcaaag atttagttcg tcaagatcaa ctacgtaata
    15361 actcccaaca atatattgat catttaggac gtatggttga tgaaaacgga aactttgtcg
    15421 atgaaaatgg tgaaatatta aaagatagta aatacggtaa agatgctaaa ggcaacttta
    15481 ttgacgaaaa agggcgtttg gtcgatgaag atggaaacct tatagatact aatggtaaac
    15541 cattggaaga tagtgaagat ggtaatattg gtatggatag cgaaggacgt ttaattgatg
    15601 aaaaaggtcg cttagtcgat gaagatggaa atcttgttga cgaaaatgga cgcaggttgc
    15661 taaatggtga ttttggtcat gatagtaacg gacgtttagt tgacgaaaaa ggtcgtttag
    15721 ttgatgaaga tggaaatctt gttgatgagt ttggtaataa acttggtaaa cacagcgatg
    15781 gatataatgt gaatggagat ttagtcgatg aggatgggaa tatcattgag acgagagatg
    15841 cgcttgtggc agatcgaaat agagaactcg aacaacaacg gcatacgcca attgctgttg
    15901 caattcctga gaatgaagaa gacgagatta ctttagatca aactcatcaa gaactgagga
    15961 atgaaatgag tggtgaggac gtgtcagaca gagtgcatcg ggaattagga gatggagcat
    16021 ctggtgaaag tcagtcaaga gagcttaaca gcaactttga taatagtgct attcggaatg
    16081 acacagtact taaagacaga gaatcgctaa caaaagaaac ggataaagaa caatcagatg
    16141 tgaagaaaaa gttcattcct actcctaatg ttaatcaaac agataaatat gaaacaaata
    16201 ttggatcagg aatgtcgaaa gacaatcaat caattagaga acctgaactg acaaataaaa
    16261 ataatagaac aaataattct aaatctactt cagataattc agggaaatct aatcaatcaa
    16321 ccaagaaaac atcaacagct aaatcagccg ttgaatatct atcaaaaact ggtaaaaatg
    16381 tttacacctc agcagataaa gctttagcaa aagctccaaa aggtagtcta gcagggataa
    16441 cctatgaagc taaaaacgaa gctattaaaa taggtaaagg aacggctaag gttgccaagg
    16501 ctactgctac aggagcatcc aaaactggta aagtagtgaa gaaatctacc atgtcagtca
    16561 aggactatat tgtaaaatac ggtaatcaaa aatcaactga aaataagtct gaagacgagt
    16621 aaaaagaagg agcattgatt tgctccttct ttttactttg atttctaact gaagatgtca
    16681 aggaaaattt ctatttatgc aaaaaaatta ttttaaattg tctataaatg aattaaatat
    16741 ttactgttca aagttgtata attaaaaagg gcttatgctg gtaaagaaca gaattattat
    16801 ctaaacaaaa acttgataaa ccagcatttt tagccaataa aaaaagtgaa ataatttaat
    16861 ggaaaatctc ttttaatcat cgaaaattta tgttattatg ataacataaa gatgtaaata
    16921 tcgagacgtc tttttcttag ttagatcatt gacaagtgaa caaataagat ttgtcgatga
    16981 tataattaac ttattaaaaa tattaaaaag gaggttttta tatgttcgat tctattaaaa
    17041 atttggttag cggaatgacg cctgatatga aaactttagt acaagttgtt ttgtatttct
    17101 tggcattcgt tttttcgttg aaaccaggat tcactgcaat gaaatttttt ggtgatgcaa
    17161 aatggggaga tggattaaaa tatgttgggg cgattattgc aatctttatt attcctgtaa
    17221 ttacaaccgt tatgctttac acaatgggta ctaaaacagg taatgatttg aaccaaaaag
    17281 ctaatatcat atcagcactt attccagttg ttggtatcta tctagtttca aaatttccaa
    17341 aaaatgaact gttaggttaa aagtgaattg aacaaatttg gataatatgc cgatttgttc
    17401 aatttacttt ttaccaaaaa actacgctcc aagcgtagtt tagaagtgag ggaagaaagt
    17461 aaatgtaaga tggtttttga tagagaaaaa atgttagctc acgccaacga agtattaatg
    17521 agcagtctga aaggtaccga actggcaaag ataatgaata tgaatgttaa ccagttctat
    17581 gattatcgaa atgggagtaa aaagattgaa aaagccagac ttgaaacgct tattaaattc
    17641 gaaaaagcat atgtatatat gctagataaa cagaaaagaa ctattgactg aaaaaaggag
    17701 ttttacaata atggcaaaat tcaaagatat taccgaatct attaattatg tggcagaaac
    17761 agttcctgta gttgcactct ctttgaagcc acaattactc gaagtgtaca aaaaagaaga
    17821 aaaagaagcg aaggcaaaaa aagctaaagc aaaggtatgg aaagaattcg atgcgttgct
    17881 gaccaaagat ttaccaaaga aaaattatat ggtaattgaa gatgtacaaa agctcaacag
    17941 tgagggagtt atagatgagg atgatgctat tctcgattct gagaagtatg tcgttctatt
    18001 gaagtcaagt ttgaattatg tagctgatct accagaaaaa tttaaagaga agctattagc
    18061 aggtgtccgt tttatggata gtagtggtca attattagtt attccggatg atcctaatgt
    18121 gtttgaatca gaagttgaag aagctacaga cttctatagc gcagtagagc aattagaagt
    18181 agctactaat ggattagaac gaattgctga gaccttcaac tcttctgaag aagttgaatt
    18241 tgaagccgta gcagaaaatg taccagatga aaaagaagct ctttttgtgg atgataacaa
    18301 accaaccgaa gcagaaaacc aagaagtaag catagaagaa acacaaatac aaacagaaac
    18361 ctaccaacaa ccaaaaattg atgaaaaacc cgaatcccaa agtgacaaag aaaattatgt
    18421 ttatgaagct gaaaaaatca catctgaaag tgatgtaccg aaaaacttag atgctgattt
    18481 tttagaaaaa aattttgaac agatcattgc tgacaaattt caacctgtag ttttagatga
    18541 gattaatgac cctgtcgatt taaacgagga gatgctatcc atacctgagt tgaaaccatt
    18601 gctgttttta gctaaagaga gtatgaacgc tcgcatctct attgctaata ataaaatcaa
    18661 cagtgaatta aataagacga aggagcaatt gatcctcaaa ggaaagcaaa tgttagctga
    18721 tgagcttttg cggatagagg aagcaacctc attaaatggt caaaatcagt ttacaaatac
    18781 actatcggtt gctaaagaga gctacgaagc agaagcatct aaaattgatg atttagtgat
    18841 ggaaaaatcc aatatcattc gtaaagatta tgatatggcg aaacaggctt atttggatgc
    18901 tgaactaaag aaactagagc aacggtatga tgctgagcat ttaccagaag ttaaacaaca
    18961 agaagatgaa tatagaaaag caactgaaga aagtcttgat caacagtacc aagaagcaat
    19021 tgaatctatt aaaaacgatg cattgaaaca aagccaagaa gcaaaagaag ttgtggtgga
    19081 acgaattatc aattcattga aaccagaaat tgaaaaatca ttaacagacg tgcaagaaaa
    19141 aactttagag attgtcgaaa aaacaaagtc agaaaatgaa gctgagttcc atgatacaaa
    19201 agagaaaaca acagatgtgg ttgtcaaata ttttgcgaac aaagaagaag ttgaaaagca
    19261 ggtagctaac aacacgaaag aattagaaac aaaacttgta gagcaacaaa aaactatcga
    19321 agaatataaa gatcgaatga aagaggtcaa agatttagca gagggttata aattaaatag
    19381 cgaaaagttg gcagaacaaa gtgaagcttt aagagaagac ttatctcgtt cccgaagtga
    19441 tctagctcaa atggcagaaa aaacgaaatc attagcagtt gctacagaaa atcaatcaag
    19501 ttcaaaacgt gattttacaa gtaaaaagat ggctactatc atttctgtcg ctgcaatggc
    19561 ttgcggaacg atcttgggat cgtcctactt tattagtcat aataatcaac aagcacaggc
    19621 tcaaactcaa acatctattt cgagagtgga aaattcgatt gggacaaata cgaactctgc
    19681 ttcaacaaat agtagctatg aagcaacacc aactacagag ttcaaagttg ggaaagatgt
    19741 tgtcatcagt attgatggga aaacaactaa ggctgaagtg atttctattg atggagaaaa
    19801 agcagttgtg aaaactccag acggaaatca gtacaaaatt caagctcact tatctgataa
    19861 taactaagga gggctattat gcgagaagaa aaaatagttt atccagtaat tcaaaacaat
    19921 caagaaaaca tgacctcttc tcagcactta caggaagcac agctcgtgta tcaggtaaat
    19981 gaactaccac aaacgaacga gacaatgagc aagtcaatat ctttaattgg cttgcttatt
    20041 gttgcaatag tgataactgt aggtgtttac cgtaagttga acgtgaaagg aagtatttct
    20101 gatggcagat aactcaaact atggatttaa acataactta catcaaaaac cttctgaaac
    20161 aattgtcaga gttgaatggc ttgaaaagca tgtcaatagc acctattctg ttacagcaaa
    20221 aagattaatt gtatcagcta tagttgttgc agtcgttgtt cttggatcaa ttgtttactt
    20281 agtttctgat acgattaaac tgaaatttat ttttgttcca acgttattaa tgataattgg
    20341 atttcttatt ctaattagcc agtatatgtt tattgatcca tctagtggtt cattgatttc
    20401 tggttctatt agactgttca aatcagtagt taattcagca agagtatcta tggggaaaaa
    20461 gaaaccattt cgaggcacag agattttaaa agtgaaacaa gatggaacaa tcgtatatcg
    20521 agatgggagt gtagcattaa tgttcaatct agatggggct acatcactaa ccgcttttcc
    20581 agaagaagtt atggctcagg aatcgaaatc aatacaatat caaaatacca gagatcgttc
    20641 cttagctgaa atcaaaatca cttcatctca acctcagaat acggaaagac aaactcgaaa
    20701 tatgaaaata caaaaacgaa atgcaaggag taacaaagca gtctttgaca taatagatca
    20761 gcaagaacga tttgtatcag aatatattga tggtgtgaaa acaacagtaa tccaatatct
    20821 tatcttgaaa tctcaatcta agatggtatt ggatgaaggt gtggaacgtt tagaaaacta
    20881 tacgagtaga ggactttatt atagtgtcac tcagttgtca gctaaacaaa caaaagaaat
    20941 attatctgat atttatgcac tgaaatagag gtgagagatt aaatggaaat tatgggtaga
    21001 tcacaactat taaatttcaa aaaaaagaga aaaactaaaa aagaacaaaa aactagaaat
    21061 tcaaaggtag tgaataatac ctttgttagt gatttaacat ctttagttgg ttatcaattt
    21121 acaccatctt acattaggac aggtaagcgg tggggaacaa ttgtgaaatt agtgaatact
    21181 tatggaatga atagaaatta tggatttggt tggatggtta aagctattcc tgaagtgaga
    21241 gtagaagggg taaaagccta tctaattgaa gcagataagc cagtgtcaca aaaagatcaa
    21301 caagaaataa tgcacgagaa tgttggggga tcaacgaata gttatgaaaa taatttagaa
    21361 ggaaaaaatt cagatgctga aattagcaaa actgttatgc ggattatgga tttgaataca
    21421 gcatctttac tagacagcat ggcaaataaa attattgatt ggaagctaga aatcctatta
    21481 gtatccgatt ctcctaataa aatcagcgaa cagctaatta aactccaacg gttgtatgat
    21541 gaaaaaatgt atggattaaa gcttatgtca tatggaggag atcaacgtga tctgtttgat
    21601 agacttttaa gtgaaccaat cgggaatgtc tatgagtata cgtctatgag ttcggatttt
    21661 gcaggaaatg atcatgcaat tcgcaaaggt ttagatgatg cgaatggatg ggctatcggc
    21721 actatgtcta ctagctacgc aagtggtgta gcttttatgg cactggatga ttcttttaca
    21781 agtaaagttt tagtggcatc acacaaaaac gctacaatta aaggctatga tgaaaaaatc
    21841 tcagcatcat cactttgggg gcaattgatt gctaataatg caatggcaaa tcataaacga
    21901 acttttcata ttgtcttaaa taaatttgat tactttggtg atccaactgg tagaacattt
    21961 gttgccaaac cttcgttaaa cagtgtaata gaaaaggtta atttggctga agaaggtctt
    22021 aactctttgg agctttttgg taatcaaaag gacattggtg atttatatag acggaatctc
    22081 gataaactaa ctcagatttt ttatttgtta gcaggcagaa cattagattc tgcaactaga
    22141 gttttattga aaaaagaatt aggtaacttt tatataagtc agaaactttg ggatattaat
    22201 tatgaagaac gttcaaaact tttggtgaac agtagtgata caatgccagt tcttggggat
    22261 tttattagct atttgactaa tgctgaaaca aaagccaaaa aatctggtac tgaaaaagaa
    22321 gtcgatcgta ttattgtatt aaaagacaca ttgagaacag cattagacag cttctctagg
    22381 atgtttaatc aaagaacgac actgaaagaa atcggtaaaa gtgacaaatt acagttttac
    22441 tataacttag gtgatctaag agcagaaacc aatgttatgg aagctcaatt tttaaatacc
    22501 tttgatttca tcacatcaca agcaaaaaaa gatgatattg ttatgattca cggcttcgat
    22561 aagttatcta ttgaaaccgt tgggctaatt aaaaaaagat taacaatgct tgaagaagat
    22621 ggaattcgtc ttgcctttct atttgataca atcggttcag gagaagacaa tgaatacaaa
    22681 gaagatattg aaacagctga tatattcaat acagatggaa aaatttacca aagttttgaa
    22741 agtgatttta attacactat cattggaaag atgaccagtg gagagttaat gaagtatgaa
    22801 aaaaaagttg ctcctgctag aaaaggagaa gatgatcaaa aactttctgt tgatttgaga
    22861 cagtatttga caacaggaag aagtaataaa tttcagatca ggagaccagt ggatctgact
    22921 aataattttg ttattggaga gtttattctt taagttttat ctttgatttt gagtgtataa
    22981 ggaaaggata agaaaaaaca tgtatatttg agactaaaaa cagtcttaga gaacatggta
    23041 cgttttgtga aaagagaact atagaaattt cataaagtga aaccttactg agtgaaataa
    23101 ttatagtgga ggttaaataa tgaaaccaaa cataaaaatt ttagatcgaa tatttctagg
    23161 tagagacaca gaagtaatcc taattcaaca tgaagaagga tttgaagtta gtataggtat
    23221 tcagaaactt caaaaacctc attattgtaa tcagttgtat aaaaacttta ctgatgaaga
    23281 aaaagcgaga gtttt
//
LOCUS       DAXWLG010000029        23182 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXWLG010000029 DAXWLG010000000
VERSION     DAXWLG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.029"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..226
                     /locus_tag="ACGE4T_000886"
     CDS             <1..226
                     /locus_tag="ACGE4T_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386188.1"
                     /translation="VAEVQKLGKSMWTEKPKRRTPRKIAIWHDDRILVTGTAEQLSEI
                     TGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            226..582
                     /locus_tag="ACGE4T_000887"
     CDS             226..582
                     /locus_tag="ACGE4T_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFF8386189.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            542..787
                     /locus_tag="ACGE4T_000888"
     CDS             542..787
                     /locus_tag="ACGE4T_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386190.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            784..1203
                     /locus_tag="ACGE4T_000889"
     CDS             784..1203
                     /locus_tag="ACGE4T_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFF8386191.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            1200..1757
                     /locus_tag="ACGE4T_000890"
     CDS             1200..1757
                     /locus_tag="ACGE4T_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFF8386192.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1754..2050
                     /locus_tag="ACGE4T_000891"
     CDS             1754..2050
                     /locus_tag="ACGE4T_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386193.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            2127..2540
                     /locus_tag="ACGE4T_000892"
     CDS             2127..2540
                     /locus_tag="ACGE4T_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFF8386194.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            2849..3001
                     /locus_tag="ACGE4T_000893"
     CDS             2849..3001
                     /locus_tag="ACGE4T_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386195.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(2998..3273)
                     /locus_tag="ACGE4T_000894"
     CDS             complement(2998..3273)
                     /locus_tag="ACGE4T_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386196.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            3727..3933
                     /locus_tag="ACGE4T_000895"
     CDS             3727..3933
                     /locus_tag="ACGE4T_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386197.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            4129..4296
                     /locus_tag="ACGE4T_000896"
     CDS             4129..4296
                     /locus_tag="ACGE4T_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8386198.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            4322..4666
                     /locus_tag="ACGE4T_000897"
     CDS             4322..4666
                     /locus_tag="ACGE4T_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFF8386199.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            4671..4952
                     /locus_tag="ACGE4T_000898"
     CDS             4671..4952
                     /locus_tag="ACGE4T_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386200.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            5055..5369
                     /locus_tag="ACGE4T_000899"
     CDS             5055..5369
                     /locus_tag="ACGE4T_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFF8386201.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            5347..7041
                     /locus_tag="ACGE4T_000900"
     CDS             5347..7041
                     /locus_tag="ACGE4T_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8386202.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            7061..8239
                     /locus_tag="ACGE4T_000901"
     CDS             7061..8239
                     /locus_tag="ACGE4T_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8386203.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            8202..8888
                     /locus_tag="ACGE4T_000902"
     CDS             8202..8888
                     /locus_tag="ACGE4T_000902"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFF8386204.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            8888..10048
                     /locus_tag="ACGE4T_000903"
     CDS             8888..10048
                     /locus_tag="ACGE4T_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8386205.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            10058..10933
                     /locus_tag="ACGE4T_000904"
     CDS             10058..10933
                     /locus_tag="ACGE4T_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386206.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFLEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYRKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            10930..11241
                     /locus_tag="ACGE4T_000905"
     CDS             10930..11241
                     /locus_tag="ACGE4T_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386207.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            11231..11584
                     /locus_tag="ACGE4T_000906"
     CDS             11231..11584
                     /locus_tag="ACGE4T_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8386208.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            11574..11975
                     /locus_tag="ACGE4T_000907"
     CDS             11574..11975
                     /locus_tag="ACGE4T_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386209.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            11968..12372
                     /locus_tag="ACGE4T_000908"
     CDS             11968..12372
                     /locus_tag="ACGE4T_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386210.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            12384..12992
                     /locus_tag="ACGE4T_000909"
     CDS             12384..12992
                     /locus_tag="ACGE4T_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8386211.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPNNMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            13012..13374
                     /locus_tag="ACGE4T_000910"
     CDS             13012..13374
                     /locus_tag="ACGE4T_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386212.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            13377..13559
                     /locus_tag="ACGE4T_000911"
     CDS             13377..13559
                     /locus_tag="ACGE4T_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386213.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            13577..16897
                     /locus_tag="ACGE4T_000912"
     CDS             13577..16897
                     /locus_tag="ACGE4T_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8386214.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            16948..17685
                     /locus_tag="ACGE4T_000913"
     CDS             16948..17685
                     /locus_tag="ACGE4T_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8386215.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            17695..19986
                     /locus_tag="ACGE4T_000914"
     CDS             17695..19986
                     /locus_tag="ACGE4T_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFF8386216.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            20010..22232
                     /locus_tag="ACGE4T_000915"
     CDS             20010..22232
                     /locus_tag="ACGE4T_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386217.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGA
                     DYSPVDGKLILIRYRYVASGTGKIVLDLPINSNSITQEWKEFSITGTTPTFSPQAYHP
                     WIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDAPHYLSKTPLGENIANKSVTF
                     PINTSAYKLYEYNTEEDLIIGQTYTITLKGTKPASQTFAVYNYWNINLGSLKPVEGLT
                     DVWSITFTPTTLEPGLPKDLRILQSPKETAGACQIDWLKIEKGDTRTPNIEQYKYRGI
                     GMRDSNNPKDYVWDLAPEYVENNLATDIKVAEITGKANNYTDGKVSQINSQLTASINE
                     VDTTAKGAQTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGT
                     GYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGRKYASWLYTDLYKDGTSVD
                     NLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD
                     "
     gene            22310..22552
                     /locus_tag="ACGE4T_000916"
     CDS             22310..22552
                     /locus_tag="ACGE4T_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFF8386218.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22568..22765
                     /locus_tag="ACGE4T_000917"
     CDS             22568..22765
                     /locus_tag="ACGE4T_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8386219.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22776..>23182
                     /locus_tag="ACGE4T_000918"
     CDS             22776..>23182
                     /locus_tag="ACGE4T_000918"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8386220.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELAR"
BASE COUNT         8189 a         3467 c         4942 g         6584 t
ORIGIN      
        1 agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
       61 gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta ctgctgaaca
      121 gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta ggagcttatg
      181 gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga tctcattaca
      241 caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga taaaaaatca
      301 aaagaacgag ctgaattagt taagttagaa atggctgaaa caggagcagg agtgcgaagt
      361 agcaggcatt ggaaagcagc agcaaacatt gaattttatt acaacgaaat tcaaaaaggg
      421 ttcgatcaga tgcgtgagct ggatcggcaa acaaattgga gcaagaaact tcatcaagat
      481 cgtttcaaat ttgtagagaa gtataaagaa atattagaag agtatttgag gaggacagca
      541 aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat caggagcaac
      601 actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc agattatttt
      661 gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag aatcaaaagc
      721 ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag
      781 cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag agatgtagcc
      841 gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc
      901 gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat
      961 aagaatggtg tggatatatt tgaaggggat atagtattag tcagcgtgcg aaatggcttc
     1021 gattacttag ataataaagt ctgtattgtc aaaaattcaa tagattattc cggattagtt
     1081 tgtgccactg ttgatgaaga cttagagtat caaattttta acacagagct gtttgaagaa
     1141 tacacgtacg aagtcaccgg aaatatatac gagaatagcg agttattgga ggaacagcga
     1201 tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agcgatagga aatgatgcaa
     1261 tagctgcttg ctataacgag agtataaatt caggtattac gttaatgaaa aaaatagacg
     1321 aaccgcagaa accagttatt ccacaactcg tggccggttg gcttgagaaa tctacggacc
     1381 cttttacaaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt gattcatatt
     1441 atttctgtga ttggtttgta cgagatggca tagtgacgca agagcaagga gaagaattac
     1501 tcgcttgggc aacaagacaa tcatatgaaa cactattgag cctatacaac ggctacgagg
     1561 ttgagaaaga gccgttatgg gcaataaaga atgccgatgg aaactatctt actaaatgtg
     1621 ctttatgggg aaaagatgga gtaaattata gttttgaatg caatccatct catcgattgc
     1681 ttttcactga taaagcaaca gcggatgctg cagcattgtt ggtgaatgga acagtggaag
     1741 aggtggtaga aagatgaaac taaaagacgg attttacgct agcagtcacg gtatcggcgg
     1801 tttaatgcta gatatgccga caaagaaccc taaaacacgc aagaaatcaa aattcaaagt
     1861 cggtgacatg gtccgctgtg aagcagaaga gttcgtttat ccgttcagag gatacgttaa
     1921 aaagatactg tcaaactcag caatcattcg tattgaaaat acaatgaagt gtgatcgttc
     1981 gactgcaaaa cacaagcatt atttagctgt agcgagatta gttgacatgg aagtaatcaa
     2041 gagcaaataa aaaaagccgg atcgctccga ctgattcaat aaatccaaca catttattat
     2101 atcacataaa ggagcggttt gacttgatgc aattgttacg agaggtagat ttcaaacaga
     2161 caagatgtaa tgcgagagat gtgctgaaga actttcggcg tttggagcgg atggcaggtc
     2221 gctctttgat agatattaag tcgccgatta taacggatat gccgaaggca ccgaagcatg
     2281 gcaataaggc agaagacgcg attattcaga tgatggatat agaaacggag agagatgcga
     2341 ttctagcggc tttgatggct cttagtctga ttagtcgtca gatactctac tacagcttct
     2401 gtgtgccaga tagcttctca aactacagaa ttagccgtga agtgggttat tcagaaagaa
     2461 gtatacaacg gatgaagtcg gaagctctaa tagagtttgc agaagcatat aaacacggaa
     2521 aaataattgc ttataaataa tttggcggtt ttttggcgga atgatggcgg tttttagaca
     2581 tttaccagtg ttattatggt agtgtcgaaa gattaggaaa caggatcgac aaaataaacg
     2641 aagggaggca atctccctca tcgttttaaa cttcttctta gatagacagc aactaattat
     2701 gaagaaaagg atgtgaattc aactcctcct aaattcttca tcggttgctg tttattttga
     2761 ttagacagca gcactcacaa aactaaacat gttcttgtcg tctttcaact gctgctgtct
     2821 attaatttat gtattggagg gaagaaaaat gaaattatca gtagaaggca ctccagaaga
     2881 aatacaagaa ttgctccaag ctattggtag tagcaaggag caaaaggtaa gtgtttctaa
     2941 ttcagatatt gacggtattt gcaattgttt aaacagagaa aaaatatcta atccttacta
     3001 agagaaaata tgttcgttaa tgaatttcca attttcctct gtagctctac cttgataatt
     3061 tttttgcagt tcacagatga ataaggaatc gttattatca agtactgtat ttaatttttc
     3121 acacatttga tttggattca aattagtttt aactaaccaa aaagattttt gtagctttat
     3181 atatgcccca aattctttta ttatttcaaa tactttatca tacttttgcc ctgggttatt
     3241 aagatcataa ctaatcatgt agggtttata catgttgtct tgcacatcct tgttttatat
     3301 ttcagcggac cactcgctga taaataaaat tatacgctta gtatttattt tcacaatatt
     3361 aatttatcgt aataactttt gagtaaatag aacaaaaaac ctgcactagt ttccgctagt
     3421 acaggcagtg actatatcgg ttgataatct agcatatatt aaaaataatt gcaagaaaag
     3481 aaatttatag tggtttatac caaattatca aagcaatgtt attttgttgc tgtctattgt
     3541 tttttaatta ttcacacgat aactaaaggt gggtgaagag aaatgattcc attaataatt
     3601 tcaatttttg cgctctgtct taatgtctat atgattggat ttaaaaatgg gcaaaataaa
     3661 aaatagtagc agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctaa
     3721 gcatgtgtgc taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagcgaca
     3781 ttaaatgcct atgacggtta cgactaccga aaaaagatcg ttaagaagct atacggtgct
     3841 acgtacggca atgtagtaag tgtgctatct gtacaccacc aagcttcggt cactgtggcg
     3901 gaagtagaag acgcagcggt aaatggcgag tagcctcgtg agagcctggt aagttctcgt
     3961 gagtggtgca atcccactcc agcgacttta agcaaccgag gcatcggcgg tttaaaaata
     4021 taggggtgcg caatttcgta cgcgttttgt gcatcgtgca agttactatt acatattaga
     4081 tcactctttg agtggtcttt ttatttttgc acaaaggagg aaacaacaat gtatagacca
     4141 caatacttag aacagaagta tgaagtaatc actgttcata aaggtaatgg cgaaaaagta
     4201 tatgagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa caatgaagtt
     4261 attccattgt atggcaaaag aatagctaag cattaaataa gattgcgaaa ggagacggaa
     4321 catgaccgag gaattctata gatggctatt acagttgaca agagaagatc gtttggttaa
     4381 gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac gagatcacta
     4441 tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg ttcatcatat
     4501 aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta tttgtttatg
     4561 tgttgaacat cataatgaag ttcatggcag atatcttaca gcattagata aacaagagaa
     4621 gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc atgatcatca
     4681 atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct tatactcagg
     4741 gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg ttatcagata
     4801 actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa gataataacg
     4861 tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat tacattgact
     4921 tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat cgcaaatttt
     4981 ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg
     5041 aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac
     5101 gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata
     5161 atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg
     5221 agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg
     5281 tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg
     5341 aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa agctatagaa
     5401 gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct agaagaaaga
     5461 gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc aatcaatata
     5521 ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca atgttttatt
     5581 tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct ttcattaatg
     5641 ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct actaaccgct
     5701 aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag ccaagcaaag
     5761 actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga taaaaaagta
     5821 tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact tcgttataac
     5881 acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt tgatgaaatt
     5941 cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg taaaattcga
     6001 gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc gcttgatgac
     6061 attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa ggatggagca
     6121 gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga agttgatgat
     6181 cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcagattt aaaacggaaa
     6241 atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac gttcatgacc
     6301 aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata tgatgatgtt
     6361 ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg aacagtcgat
     6421 tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga taaagatgtt
     6481 tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa catcaaacga
     6541 gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg aaaaaatata
     6601 gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta tattaaaaaa
     6661 atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt
     6721 actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga tctggttgat
     6781 gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg ttggtattgc
     6841 atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa aatagaacct
     6901 gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt tttagatgat
     6961 atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac aggaactgga
     7021 tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc
     7081 ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt gtaaattgca
     7141 agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga ttgcaaatgc
     7201 gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga atgatcttta
     7261 ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta gaaaaaaact
     7321 gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta ataattctag
     7381 cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag atgatgtgtt
     7441 ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa gtgatgttat
     7501 ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt atcaagcgta
     7561 tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt
     7621 actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa tcaataaaat
     7681 gatgacatca caatttaagg cttttatgga agctgataat gcaggtgcgg tatttcaatt
     7741 acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt caagagatat
     7801 aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg ttccgaaaaa
     7861 cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt taatgttcga
     7921 aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct atgaagtaga
     7981 agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga ttactagctt
     8041 caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta caatcaacga
     8101 aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg aaatatttat
     8161 aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat
     8221 ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga tttattggtt
     8281 ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg gatagtctag
     8341 gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta tttcaaggga
     8401 ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg attaacggct
     8461 tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa atctacaaca
     8521 atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc gatgacttcc
     8581 gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca tataaaacac
     8641 gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg tttatggatg
     8701 cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa aatagctcag
     8761 gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa
     8821 gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga caaggagatg
     8881 taaaataatg acagttaaaa atttaaaagg tgtaacagct gcaagcgacc aattgatgaa
     8941 agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct tatctaagga
     9001 aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat tagtattaat
     9061 gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg aagtagtgaa
     9121 aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg aacgtgtatt
     9181 tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta acacaactgg
     9241 tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta aactttgcga
     9301 agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc agttcaagct
     9361 atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat ggttagatcg
     9421 ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag caattgttga
     9481 tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac aaactagcgg
     9541 agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa ctatgggcgg
     9601 tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga tttatcgtaa
     9661 tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt ctaaagtatt
     9721 cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc cagtaccagt
     9781 ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg tagcctcaga
     9841 ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat accattttgt
     9901 tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta aacgtaacga
     9961 tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta caaaaccaac
    10021 aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc tttgtcagcc
    10081 ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga agaaactagg
    10141 aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta tttttcttga
    10201 gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca aagagtatat
    10261 tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt cgcgtgagga
    10321 cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta atttgcgcaa
    10381 attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc taagtcttga
    10441 ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa acattgtaaa
    10501 tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac agtgggttat
    10561 taaccgtcct aaagcgaatt atcggaaaaa ggtttatctg gattctaatc ggctatatcg
    10621 ctaccttaga gggcaaaaaa caaatataca aatctggaca gccgagatgt gggcacagct
    10681 ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag acttttgttt
    10741 tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg ctggagtaac
    10801 aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc cttttgatga
    10861 agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag caattaaggc
    10921 ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt gaaggataat
    10981 atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc taaaaatgct
    11041 gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag cttagaatac
    11101 ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga gtttgaacaa
    11161 aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga atatattgca
    11221 ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg gttggttaaa
    11281 gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg tagaagatac
    11341 agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata taacagctat
    11401 ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc caatcgccaa
    11461 gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca acgttatcta
    11521 tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg aatatggtga
    11581 ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc aaaacttcct
    11641 tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat gagctacctg
    11701 atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact gagccaaaat
    11761 atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa gatttaacag
    11821 gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt cagagaatgg
    11881 atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg gtatttacgt
    11941 ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct aaaaataaat
    12001 ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga acgactgatt
    12061 aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac agaaaaaatt
    12121 cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag tcgtccactt
    12181 aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt tgagtattta
    12241 aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga atttatgaga
    12301 agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca attcgataaa
    12361 ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca acgttcgata
    12421 acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca atcgcaacga
    12481 attgtaatgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag
    12541 cgacagaagt aaaatcaaaa tctaaaccaa acaatatgac ggtaacaatt actgcacatg
    12601 taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt attaaaccag
    12661 gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct gcagagatcg
    12721 tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact tcgaatacag
    12781 gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa
    12841 aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca gaacttgaag
    12901 aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa aaaacttcag
    12961 ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa
    13021 gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc taaactgaat
    13081 atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc aaaaatgtcg
    13141 atgggaaaag gctctatgga tattacagtt attcaaggtg caaaagccgt atatgtagct
    13201 tatcgtcaag cgaacatggt cgattacatt tcattcgatg aatttataga taaatgggat
    13261 tttgatatgg aggttgcagt agctgtatac agtactatga tgttcaaaca agcacgtgat
    13321 gcctatcaaa aagaattcga aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc
    13381 caaagctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac gttttttcta
    13441 tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa atggcttaca
    13501 acaagagcgc ttatgaagga tggaagaact atgcaataaa tcaatcccag aaaaactaaa
    13561 ggaaaggagg taaaaaatgg ctaaaaagag aacggaagca gaagtaactt tcatagctaa
    13621 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
    13681 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
    13741 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
    13801 tagtcaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg aagttcagaa
    13861 acttgaaaga gaactgatta atcaacaaac agctcagcaa aggttatcta atgatataga
    13921 taaaacgagt aaggcattgg ctcaatctaa aggtgaattt aaaacgtatg agtcaactat
    13981 gaaagactta gataatgagc aacaacatct aaagtcaagc gcatcattag tagaatccga
    14041 atataaaaaa tggcaagcaa ctgctggcca gtctgctaca gaatctgaaa aattagcaaa
    14101 agcacaagaa tatgtcggca aacagagtga tattgctgaa caaaaaatcg aagtgctaaa
    14161 aaaacaatta gatgccacac aaaaagaatt tggagaaaca tctaccgaag ctttagaaat
    14221 gaaaactaag cttaatgatg cggaaagaga attcgaagaa ttaagtaatg ctgctaaaaa
    14281 cgttgatacc tccacagtag atgatatcgg caaaaaactt gatatgggta atttaatgga
    14341 agctactgat cacttgtcag tgatcggaga taagcttatt gatgtaggta gtaagtctat
    14401 tgaagcagct ggaaaagcac aagctatgca ggcccaattt aaacaagtct ttggctcttt
    14461 agaaggggaa gcacaggacg ccgttgaggg aatggctgaa gaatttggaa tgttaccaaa
    14521 tacgatcaag cctgttttta cacaatatac gtcaatgttt aaaggacttg gatatgatac
    14581 caaagaagct atggagttag ctggtgatag tactcagtta gcagcagatg cagcagcttt
    14641 ttacgataag tctatggatg atgctagtga atctcttaat tcatttataa aagggaacta
    14701 cgaaggtggg gagcaaatag gtttatttgc taatgatact caaatggcag cttatgctgt
    14761 taagcataat ttgataccag cgactgaagg agcaaaaaaa gccagtgaag agtcattgtt
    14821 agctgttgaa aaagcacaat ctaagtatgc tgatgctatt aagaaacatg gtgaaggatc
    14881 tttagaagca agagaggctg ctttaaaact taaagatgcg caagataaaa taaatgaaga
    14941 attaggccca caaacgcaaa aatggtctga tttagatgag gctaccaagc aagcagttcg
    15001 agttcaatat gctgaagata tgcaaaaatt agccggtgct acaggtcagg ctagtaggga
    15061 gtctgatggt ttagaaaacc aaatgacaag agcaaagcaa gcgttagaag atttttatgc
    15121 ctcattgggt gaagatatac tgcctgtgtt tatcaaaggg ttgcaagcag gagcgaaagc
    15181 tttgcaagga ttagccgaat ggtggagtaa acttgatggg ccaatgaaaa atttcatttt
    15241 agctcttgga ggaattctag cattattaag cacattagct cctgttataa ccgcagttgt
    15301 tacgatagtt ggcacatttg gttctacagt tttgctgcca ataataggaa ttatagcggg
    15361 cgttgcagct gtaatagcga ttgtaattac agcgtttcaa aactggggcg caatcactga
    15421 ctggtttagt gatttatgga aaaaatttac cgattggtta ggtgatacct gggaaagtat
    15481 aaaagaaagt gcctcatcag tttgggatgg agtcaaagaa acctggtctg gatttgtaga
    15541 ttgggttcaa gaaatttggc aaggagtttc tgattggttt ggagagttat ggagcggatt
    15601 agttgaagga gcttccaaca tctggcaagg agtccaagag acttggcaag cattcgttga
    15661 ttgggtttca aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat
    15721 tgtaggaatt gttcaaatac catggacatt aataacgtca ttgattcaag ctggtattaa
    15781 tattatcgtg ggtatttttg atgtagctgg acagttatta ggcgcagctt ggcaagctgt
    15841 ttggacacct atttctgatt tccttaaaaa tacttgggat actatgacac aatggataag
    15901 catcgcttgg aatggaattg taactacatt ccatactata tttgatccag tagtggcatg
    15961 gtggaatggt atatggacat ctattagtac tacggcttca aatatttgga actcaattag
    16021 tgcaacagct tctagtattt ggaatagtat caagaataca atcactagct tggtacaagc
    16081 agctgctaca gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg
    16141 gaattcaatc agctctacag catcaaatat ctggagtagc gtgactagca gtataagcaa
    16201 tgctataaac gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat
    16261 cagcggaatt tggaacggta tcaaaaacac tgctttgaat ctttggaatg gaattacaag
    16321 cactattagc tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct
    16381 aacaggtatt gtttccgaca tattcaataa tgttaaaagc acgatagcta atatatggga
    16441 aggtatcaaa aagactgtta gcgctccgat tgattggatt agagataaaa tcagtagtgt
    16501 ctttgataat ttgaatattt cgataccaca tattccgtta ccacatttta aattgagcgg
    16561 agaattcaat ccattgaagg ggaaaatccc aacattgggt gttgattggt atgcgaaagg
    16621 tagtgtgttt aattctccaa atattatcgg tgtcggtgaa gcaggacctg aagcagtttt
    16681 acctttgaaa agatctgtgc tgcaagaaat tggggatcgt atcttgagta gcacatcagt
    16741 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    16801 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    16861 aaaagttcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    16921 ttttagtttt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    16981 tttcctgatt atttgcataa attggcttta gcggaaagac caaccatacc ttctccaaaa
    17041 agacagagag ttgaaacttc tgttttagga cggctaggtg gcttagtaca agattactcg
    17101 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    17161 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    17221 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    17281 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    17341 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    17401 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    17461 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    17521 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    17581 aacaaaatga agaccatgca atatccgaca ttgcaagtgg atatcaatca ttttaattgc
    17641 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    17701 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    17761 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    17821 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    17881 gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    17941 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    18001 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    18061 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    18121 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    18181 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    18241 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    18301 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    18361 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    18421 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    18481 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    18541 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    18601 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    18661 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    18721 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    18781 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaacggt
    18841 ggcgaaatca gtgatttact caatgatatt gtagatcatc aaactgatat gattactggt
    18901 caaaatggtg gttatgtttt attagatcct aaagaagcgc ctagtcgtat tttgattatg
    18961 gacacaccag ataagaatac cgcaaggaat gttttacaaa tcaacaatgc tggtattggt
    19021 ttttctaaaa ctggcattaa tggaacatat gaaacggcat ggacgttaga tggcggattc
    19081 aatgcctcgt ttattacggc tggtgaaata gtaggaatta ctattagagg tactacatta
    19141 attagtgatg gtgctgatta tagaacaagt attgctaatg gcaaaatgac ttggtactca
    19201 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    19261 ggtattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    19321 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttttatc ttttggtgcg
    19381 ccaaatttca gatatagcaa tgctagttat gtaacttctg gcgatggtaa ttctttgggt
    19441 atagatggta gtgcgggtaa ctcatgggaa tttaaggtag ctggtaggac tatgaaattt
    19501 actagtgatg gtatgctaac gttaccaggt tgtttttttg gttcatggga agatgggaaa
    19561 cttgctaggt ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    19621 aaaaactcaa ctgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    19681 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgacgatgg taaagttcgt
    19741 gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg
    19801 acacctacag aactagtttt gtgtgctgtt actcatgaag atattgacca tttcatcatt
    19861 gaaactagta agccaaacgt attagttaga tggaatttag tggcacaccg tctagggtat
    19921 gaagatatta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    19981 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    20041 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    20101 ttacggtgac tgttaaacca tttttaaatg gcttgcagat ggatacgagt ggcggaacat
    20161 ttactttaaa aggaacaaca ccatctaacc gttatgtaga tagtgttgca actagcgtaa
    20221 ctagtgaaga agtcatattt tctcttgatg gcacatttat gagtgaagca ggatattata
    20281 aacactgcta cgtagaatat agaaaaggta atcaaatttt aacaacgcaa gacatcattt
    20341 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatac gtttcgcaat
    20401 tggaagagtt gattcgaaag tataacgaaa cttttgatgc ttttatggct gaaattaaag
    20461 gtagagtgga tagcttaaat caacagatta ctgatttaac tggtcaagct aaaacgctac
    20521 aagacaagtt agatgctctg aaagaagaaa tttctaagtt aggtaactta caagtgatgt
    20581 acagcaacag catcgacttc ggtgactatg attattcggg gaacccgaat ttgttaagta
    20641 agctatcata cgacttaatt gaaaatcaaa atacttcagc tggaacactt tctaagggtg
    20701 aaaactcgtt taaatataat aagatatcag ctgaagtgga aggtggagta gagttatatt
    20761 ataaacgaag aggtatagct aactggttac cctctaataa aacgcttgta atgactgtta
    20821 agcttagagc tggagcggac tatagtccag ttgacggaaa acttatactg attagatata
    20881 ggtatgttgc cagtggaact ggcaagattg ttttagactt acctattaac agtaattcga
    20941 taactcagga atggaaagag tttagtatta ctggaactac tccaacattt agcccacaag
    21001 cataccatcc ttggatacaa tttagggctc aagatggggt acttggggaa atagaaatga
    21061 gctatgacat caaaatcgaa gaaggctcaa cagctacacc ataccagcct aatttgctgg
    21121 acgctccaca ttatttaagt aaaacaccat taggtgaaaa tattgctaat aagtctgtga
    21181 ctttcccaat taacactagc gcgtataaac tatatgaata taatacggaa gaagatctta
    21241 taataggtca aacgtatact attacgctta aaggaacaaa acctgcaagt caaacctttg
    21301 cagtgtataa ttattggaac attaatcttg gaagcttaaa accagttgag ggattgacag
    21361 acgtatggtc tataacattc acaccaacga cacttgagcc aggtttgcct aaagaccttc
    21421 gcattcttca gtcgcctaaa gaaacggccg gcgcatgcca aattgactgg ctcaagattg
    21481 aaaaaggcga cacacgaacc ccgaatattg agcaatataa ataccgagga atcggcatgc
    21541 gagactcaaa taatccaaaa gactatgtct gggatttagc accagaatat gtagaaaaca
    21601 atctggctac agatatcaaa gttgctgaaa ttactggtaa agcaaacaat tataccgatg
    21661 ggaaagtatc gcagattaat tcgcagttga ctgcttcaat taatgaagta gacaccacag
    21721 ctaagggtgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa ttagacaata
    21781 agatcgatga acgcattaat gatacagcta ctaccgcatt ttatggcgca caaggtgaag
    21841 tctctaacaa aacgaaagta gcggagtatg gagtaggaac agggtatact actacagctg
    21901 taggagaaac acatttcgaa cgtcaatcta acggatacgt taaatgctta aaagctggta
    21961 agtattcaat tagtgcgcaa atacgtgtgc aattgggcag gaaatatgct tcatggttat
    22021 atactgattt atataaagat ggaacttctg ttgataattt agtatcttat ggggtcgacg
    22081 ccttgcaaaa tagatttgca gctagcggaa acattgtaac tgatttagaa gtaaacgatg
    22141 tactaaatat gcgtactgaa atcggcgttg cagatggaat tttacaattt acatcttgcc
    22201 gtactttagt cttgactaga ctatctgatt aatacaatat ttataatttt gctatcaaca
    22261 cgctcaaagg agggtgtttt ttattttgca atgaaaggag gctagttggt tgaaagacga
    22321 agcaatacaa gacgtggtag aacgcttagt gcgtattgaa acgaaactgg ataattacga
    22381 attattacgt gaaaaagcgg aaagtgcaaa agatagagcg gatcaggcat attctattgc
    22441 gcttaataat gcagaagaca tcaaagagat gaaagccaat aataaatggt cgtggggtta
    22501 catgatcggt ttaggcatta caatcattgg ctatttcttg actaaattgt aaaggaggtg
    22561 agaagaaatg gttttaccag ataagtatta tcaagtcatt aaatggacag ttttaacagt
    22621 attgccagct gcatctgtat tagtagcgac gttagggaaa gcgtatggat ggaatggaac
    22681 agatatgaca gtactcacta tcaatgcagt agcaacattt ttaggtgtta tcactggtgt
    22741 gtcggcttat aatttgaaaa aataggagga aacaaatgaa aaagaaaatt actattactg
    22801 cgatgagcct attaacggct ctttttttat tgccaattaa tgggtttgcc tatactatca
    22861 acaatgaatt taatttgggc gcaaatgaag gtagctcaca agtagcaaat aatcagtaca
    22921 ttttactgca tgaaacggct aatgaaacag caacaggacg caatgaagcg cagtatatgc
    22981 aacgttcatg gactagtgct tacactgctt acattgtggg tgatggcgga attgtttacc
    23041 aagttggaca acctggttat gtacagtacg gtgctggttc gtatgctaat gccaacagtc
    23101 ctgtgcagat tgagttacaa cacacacata ataaagcaac ttttgaaaag aactataaag
    23161 catacgttga attggcgaga ga
//
LOCUS       DAXWLG010000030        22821 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXWLG010000030 DAXWLG010000000
VERSION     DAXWLG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.030"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            3..371
                     /locus_tag="ACGE4T_000919"
     CDS             3..371
                     /locus_tag="ACGE4T_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFF8386221.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACGE4T_000920"
     CDS             551..1492
                     /locus_tag="ACGE4T_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFF8386222.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFIALIK"
     gene            1561..2292
                     /locus_tag="ACGE4T_000921"
     CDS             1561..2292
                     /locus_tag="ACGE4T_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8386223.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACGE4T_000922"
     CDS             2436..4616
                     /locus_tag="ACGE4T_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFF8386224.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACGE4T_000923"
     CDS             4636..5118
                     /locus_tag="ACGE4T_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFF8386225.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACGE4T_000924"
     CDS             5134..5895
                     /locus_tag="ACGE4T_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8386226.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACGE4T_000925"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACGE4T_000925"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFF8386227.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACGE4T_000926"
     CDS             7667..8152
                     /locus_tag="ACGE4T_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFF8386228.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACGE4T_000927"
     CDS             8145..8828
                     /locus_tag="ACGE4T_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFF8386229.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACGE4T_000928"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACGE4T_000928"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFF8386230.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACGE4T_000929"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACGE4T_000929"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFF8386231.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACGE4T_000930"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACGE4T_000930"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFF8386232.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACGE4T_000931"
     CDS             13125..13457
                     /locus_tag="ACGE4T_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8386233.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACGE4T_000932"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACGE4T_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFF8386234.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACGE4T_000933"
     CDS             14283..15656
                     /locus_tag="ACGE4T_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8386235.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACGE4T_000934"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACGE4T_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFF8386236.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACGE4T_000935"
     CDS             16711..17151
                     /locus_tag="ACGE4T_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFF8386237.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACGE4T_000936"
     CDS             17295..17996
                     /locus_tag="ACGE4T_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFF8386238.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACGE4T_000937"
     CDS             17993..19114
                     /locus_tag="ACGE4T_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFF8386239.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACGE4T_000938"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACGE4T_000938"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFF8386240.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACGE4T_000939"
     CDS             20493..21401
                     /locus_tag="ACGE4T_000939"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8386241.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACGE4T_000940"
     CDS             21436..21708
                     /locus_tag="ACGE4T_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFF8386242.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACGE4T_000941"
     CDS             21719..22765
                     /locus_tag="ACGE4T_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFF8386243.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7696 a         3980 c         5011 g         6134 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttatagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag a
//
LOCUS       DAXWLG010000031        21565 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXWLG010000031 DAXWLG010000000
VERSION     DAXWLG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.031"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            136..1902
                     /locus_tag="ACGE4T_000942"
     CDS             136..1902
                     /locus_tag="ACGE4T_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFF8386244.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1917..2354
                     /locus_tag="ACGE4T_000943"
     CDS             1917..2354
                     /locus_tag="ACGE4T_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8386245.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2367..3518
                     /locus_tag="ACGE4T_000944"
     CDS             2367..3518
                     /locus_tag="ACGE4T_000944"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFF8386246.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(3580..4278)
                     /locus_tag="ACGE4T_000945"
     CDS             complement(3580..4278)
                     /locus_tag="ACGE4T_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFF8386247.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(4271..5623)
                     /locus_tag="ACGE4T_000946"
     CDS             complement(4271..5623)
                     /locus_tag="ACGE4T_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFF8386248.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(5846..7246)
                     /locus_tag="ACGE4T_000947"
     CDS             complement(5846..7246)
                     /locus_tag="ACGE4T_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8386249.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            7407..8144
                     /locus_tag="ACGE4T_000948"
     CDS             7407..8144
                     /locus_tag="ACGE4T_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFF8386250.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            8269..8748
                     /locus_tag="ACGE4T_000949"
     CDS             8269..8748
                     /locus_tag="ACGE4T_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFF8386251.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            8745..9512
                     /locus_tag="ACGE4T_000950"
     CDS             8745..9512
                     /locus_tag="ACGE4T_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFF8386252.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            9742..10311
                     /gene="lepB"
                     /locus_tag="ACGE4T_000951"
     CDS             9742..10311
                     /gene="lepB"
                     /locus_tag="ACGE4T_000951"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8386253.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10415..11017)
                     /locus_tag="ACGE4T_000952"
     CDS             complement(10415..11017)
                     /locus_tag="ACGE4T_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8386254.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            11123..11353
                     /locus_tag="ACGE4T_000953"
     CDS             11123..11353
                     /locus_tag="ACGE4T_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386255.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            11344..11592
                     /locus_tag="ACGE4T_000954"
     CDS             11344..11592
                     /locus_tag="ACGE4T_000954"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386256.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            11698..12282
                     /locus_tag="ACGE4T_000955"
     CDS             11698..12282
                     /locus_tag="ACGE4T_000955"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFF8386257.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            12285..13358
                     /gene="prfA"
                     /locus_tag="ACGE4T_000956"
     CDS             12285..13358
                     /gene="prfA"
                     /locus_tag="ACGE4T_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFF8386258.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            13351..14190
                     /gene="prmC"
                     /locus_tag="ACGE4T_000957"
     CDS             13351..14190
                     /gene="prmC"
                     /locus_tag="ACGE4T_000957"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFF8386259.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            14183..14362
                     /locus_tag="ACGE4T_000958"
     CDS             14183..14362
                     /locus_tag="ACGE4T_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386260.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            14384..15409
                     /locus_tag="ACGE4T_000959"
     CDS             14384..15409
                     /locus_tag="ACGE4T_000959"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFF8386261.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            15439..15567
                     /locus_tag="ACGE4T_000960"
     CDS             15439..15567
                     /locus_tag="ACGE4T_000960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386262.1"
                     /translation="MIQCYQVNLLSVNEKLSVTNRNRRDTNIWQFVFDGKNKIVGK"
     gene            15584..16828
                     /gene="glyA"
                     /locus_tag="ACGE4T_000961"
     CDS             15584..16828
                     /gene="glyA"
                     /locus_tag="ACGE4T_000961"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFF8386263.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            16981..17928
                     /locus_tag="ACGE4T_000962"
     CDS             16981..17928
                     /locus_tag="ACGE4T_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFF8386264.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            18062..18691
                     /gene="upp"
                     /locus_tag="ACGE4T_000963"
     CDS             18062..18691
                     /gene="upp"
                     /locus_tag="ACGE4T_000963"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFF8386265.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18830..19072)
                     /locus_tag="ACGE4T_000964"
     CDS             complement(18830..19072)
                     /locus_tag="ACGE4T_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386266.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            19193..19265
                     /locus_tag="ACGE4T_000965"
     tRNA            19193..19265
                     /locus_tag="ACGE4T_000965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19226..19228,aa:Thr,seq:cgt)
     gene            complement(19384..20022)
                     /locus_tag="ACGE4T_000966"
     CDS             complement(19384..20022)
                     /locus_tag="ACGE4T_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFF8386267.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(20145..20777)
                     /locus_tag="ACGE4T_000967"
     CDS             complement(20145..20777)
                     /locus_tag="ACGE4T_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8386268.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            21128..21427
                     /locus_tag="ACGE4T_000968"
     CDS             21128..21427
                     /locus_tag="ACGE4T_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8386269.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         7355 a         3790 c         4326 g         6094 t
ORIGIN      
        1 ttctgatacc gaaatctttt tattttgctg acatactcaa acctttggca caaagtttcg
       61 tgccaaagta cgactatttc tctttttagt aagcggatac aattagagta tcaaaaggag
      121 agagggagag acaaaatgga acggacgatt cccaaaaaca acatttcgct aaaagcgagg
      181 gtacaaaaat taggcagtac tttatcaagc atggtcatgc caaatattgg agcactgatt
      241 gcctggggtg tattaacggc tctatttatc ccagatggtt atttaccaaa tgaggctttc
      301 gctaccatgg taagcccaat gctaacttat ctgattcctt tattgatcgg ttatactggc
      361 ggaaaagtaa ttgctggtga ccgcggatca gttgttgggg caattgcaac gatgggtgtg
      421 attgtcggaa cagatattcc aatgatgcta ggcgcgatga tcatgggacc tcttggtgga
      481 tatgccatca agaaatttga tcagctattc caaaaacgga tcaaatccgg ttttgaaatg
      541 ctagtcaata atttttcagc aggtcttatc ggttttggat tagcgttatt aggtttttct
      601 gcgattggtc cagttgtcga tgcattgact caagcaatgg caaaaggtgt agagatcatt
      661 ttatctgccc atttgattcc attgacaagt atttttattg aaccagcaaa aatcttattt
      721 ttgaataatg caatcaatca tgggattttg acaccattgg gaactgaaca agtagcagag
      781 gctggaaaat ctattctatt cttgcttgaa gcaaatcctg gtccaggact tggcgtatta
      841 cttgctttta ctttgtttgg caaaggagca gcaaaatctt cagcaccagg agccatgatc
      901 attcactttt taggcgggat ccatgaaatc tattttcctt acgttatgat gaaaccgtta
      961 ttattcttat cagttatagg cggaggaatg tcaggaagtt tcattttcca attgatggat
     1021 gctggactaa gagcgccagc ttctccaggg tcgatcattg cgattctagc gatgacaccg
     1081 ttcaattctt accttcctgt tattttgggc gtgattgttg ccgcagctgt ttcgtttggt
     1141 atttctgcag taattttgaa agcggatgcg aaagatacaa atgaagactt cgaaaaatct
     1201 gttcaagaga caaaggcagc taagcaagct tccaaaggtg cccaaggtat tcctcaagga
     1261 gctacattct ctggcgtaca aaaaatcatt tttgcttgtg atgcaggaat gggatcaagt
     1321 gcgatgggcg cttcgatctt gagaaaaaaa gtacaggaag cagacttgcc tcaaacggtg
     1381 accaatcaag caatcagcca actgacagat gactcgaata cattgatcgt tactcaggtc
     1441 gaactacaag aaagagcaaa acaaaaagca ccgagcgcgc gttttgtctc agtagaaaac
     1501 tttttgaatt cacctcgata tgatgaaatc attcaagaat taagcagcaa gcagaaaaca
     1561 tcagtttctg aaattgagaa tgagccagca gaagagtttc tttcagcaaa catcaaacaa
     1621 atcaaagaga ttctacttgt ccacgatgat cgttccggat cagcaacaat ggggatgacc
     1681 gtgctcaaag atatattaga caagaaccat ttaactatcc cgttaagaaa agtcaatatc
     1741 caagatttaa aagatgaacc tgattcactg atcgtagcca aagaaagttt agcggaaaaa
     1801 gtacagatgg ctgcacctca atccattcat ttatcagtca acagcatggt caatccgcaa
     1861 aaatacgaat cgattgcaca atatttgaaa cagacagctt aagggaggaa aacagcatgc
     1921 aaagtttgcc attcgacatg atcgagctca atcgatcttt taaaaccaaa gaagaagcga
     1981 tccgctattg cggtcagatg cttgtagaag cagggtgcgt tgatgaggaa tatatcgatg
     2041 ccatggtaga tagagacaaa atgttatctg tttatatggg gaattttatc gcgattcctc
     2101 atggaacaga tgaagcaaaa aaatatgtga aaaaatccgg catctgtgtc gttcaagtac
     2161 cagatggtgt agattttggt accgaagaag aagaaaagat tgcgactgtt ttgttcggta
     2221 ttgcaggagt cggagatgag catttgcaac ttgtacagca aatcgcactt tattgcagtg
     2281 atatggacaa tgttgtccag ctagcagacg cgctcagcaa agaagaagta acggaaaatc
     2341 tagcaattgc ttaaggagag agaaaaatga gagcagtaca ttttggtgca ggaaatatcg
     2401 gtcgaggatt tattggagag gttttggcag ctaatggatt tgagattact ttcgtagatg
     2461 tgaatcacac gatcattgat gctttaaatg aaagaaaaga atacgaaatc gaattagcag
     2521 atgaatcaaa agaaaaaatc actgtaaaaa atgtcaaagg aatcaacaat caaacaaatc
     2581 cagaaaaagt aatccaagaa atcgctgaag cagatttagt tactaccgcc attggcccga
     2641 atatcttacc ttttattgct gagttgattg caaaaggtat ccaagaaaga gaaaaggaag
     2701 gaagcacaac atctttagat atcattgcct gtgaaaatat gattggcggt tcagcatttc
     2761 tagaaaaaga agtgtataaa tatcttccgg aaacatcatt tactgacaaa tatatcggct
     2821 ttccaaatgc agcagtcgat cgaatcgtcc cattacaaca ccatgaagat cctttattcg
     2881 tccaagtgga acctttcaaa gaatgggtca ttgacgatag ccaaagaaaa aataaaaaaa
     2941 tccaattaaa aggggtttta tatgtagatg atctagaacc atatatcgaa cgcaaactat
     3001 ttagtgtcaa tactggtcat gcgactgtcg cttatacagg tgccttgtta ggttaccaga
     3061 ccattgatga agcaatgcaa gatgcgttag ttgtggcaca attaaaatca gttttgcagg
     3121 aaacaggaag tttgctgatt gccaaatggg ggtttgatgc agagcagcat catgcgtata
     3181 ttgaaaaaat cattcatcgc ttccaaaaca aaaatatctc tgatgcgatc acacgtgtcg
     3241 ctcggacacc attacgtaag ttaggttatc aagaaagatt tacacgtcca gtgagagaac
     3301 tacaagaaca taacttgact tgcccacatt tgacggcaac aatggggatt atttttaatt
     3361 actatgatcc tgaagatgaa caaagccgtc aattgcatga aatgaagatc catgaaaact
     3421 tggaacaatt gattcaagaa gtcacaggaa tcaatgatcc gaaaacaatc gggaatatca
     3481 agcaaaacgt gaaccgttat gccaagcaag tcgcttaaaa taggatacga aaaaggggaa
     3541 tgtgacgaaa tttttgtcac attccccttt ttcgaataac taggctgttt gtccttcaag
     3601 atagggcaga tccaattctt taccgtaacg ttgttctaaa gctaggcgga tcaaacgagc
     3661 tgccaggttc tcatttcttg caaggatagg tccatggaaa taagaaccga agacatttcg
     3721 gtaaatgaca ccttcagatt ggtcttcacc attatttcct tgaccttgta ccactttccc
     3781 caaaggtcgt tcaccttcac ctaagaaagt ccgaccattg tggttttcaa atccataata
     3841 agtttcattg aattcttcgt tatggataac gatatcaccg ataaaccggt tattttcctg
     3901 acttaacgtg tagtgatcca gtgcactgat tccttggatc ttttctccat tagctcccat
     3961 gtagtagtga cctagcaatt ggtagccacc acaaatggct aacatgacgc catcattttc
     4021 gatatagttg atcaaagaat ctttcttttt ctggatgtct tttgaaatga ttaattgctc
     4081 aaagtcttga ccaccgccaa agaaaaccaa gtcatattta tcagcatcaa aaggttcatg
     4141 tatactaacg atctccgtat ggcaagaaac ccccattttt tctgctatat atttcaacat
     4201 caaaaggttt ccgttatcac catatgtgtt catcaaattt ccataaaggt gggcgatatt
     4261 gagttcataa ttagccacgg ttcattcctc ctttgataaa tccttgagca gctagttctt
     4321 tacgaagctg caatacagca gtgtaagtag ctaaaatata gacatgctca gttggcaatt
     4381 ctttgatttt ttcgattact tctggtaatt cttttgtttc tgtcaattta ttttctggaa
     4441 tcccggcaac ttttagacgt aaagtcatat ctgtatgacg gtctcctcca gaaataacag
     4501 ctgggatatc catatcggca aagttttcaa aattaccatc ccaaatccag ctgacatcga
     4561 ttccgtctgc gtaattggca tttaataaag ccactaaaga aaatggataa ggagctagac
     4621 caatcatatc gattacttga ttgataccta caggattttt gacaagtact aatgtacatt
     4681 ttttcccatt gatattgatg acttcttgtc ggccgaatac tttctcgtca tatcctaaac
     4741 cttgtttgat tttatcagga tcgacattga agtactctgc cactgcagta gctgctaaag
     4801 cattgtagac attatacata ccgccaactt cgattccgta ttcatgacca tcgatcacga
     4861 aatctgctga cacattgtcc atccgtacca tatcagttaa tcgataatcc aattcaggac
     4921 gtttgaagtc acaatttgga cagtaatatt tccctaagtt tgcataagtg atcattttat
     4981 aatgcaagat gtgatgacaa tttggacata agacaccgtc tgtattgtaa tgagccattt
     5041 gttcacgatc ttcttcatgg tcaaagccat aatattttct aggattgact gtttcgatag
     5101 aattgaagat tggtgaatca ccgttactga taatcgttgc atttggtgac tttgcggcac
     5161 cgtctacaat cattttatag gtcgtataga tttctccata ccgatccatt tgatcacgga
     5221 aaatatttgt aaataagaac agttctggtt tcatatattc tgtgactttg ctcaaacttg
     5281 cttcatctat ttctaggaca gcaaactttt tctgtccttt ttttggtttt gcctgcaaga
     5341 aagtcgatac aattccctgt accatattcg caccagtagg gttggttaga acttcatcga
     5401 attgctggcg caaaatattg actgttaaag cggtagtcaa tgttttaccg tttgttccgg
     5461 tgacaacgac gacttggtag tcttttgcca aactatctag tatatgtgga tcgactttca
     5521 gcgctagttt acctggtaaa cttgatccgc ctttaaaaaa agtttttaat gcccattgcg
     5581 acgtttttcc aactgcaatc gcaaaatgac tgcgtattcc cattatgtaa cctccaaaaa
     5641 cgatttgctg acgaataaat tcaactaatc ataccatatg ttgaaaaaaa ggttaaattg
     5701 cttttcttat tcttaagaaa aggttacggc tgaaagtgca tgaaatcgga caaaatcgca
     5761 tgatttaaca aaaaaacagc ttgaaacaaa atgattgttt caagctgctg tgattttatt
     5821 attccctctt acctatattc ccattttaac cgataataat tttctcgctt ggatattcat
     5881 agcctagatc tcgtgtttct tttggcacga ataacatcaa tgtatatagc attccgatcc
     5941 gcccaataaa catcagtgta gcaatcgtga ttttgcccac aacagacaag tctccggtaa
     6001 tccctaaaga taaaccagtt gttccaaacg cagaagttac ttcgatgatg atcgagatca
     6061 gcgtttgttc ttctgtagca gataaaaaaa cgatacaaaa gaaacacata ccaagagaaa
     6121 gcataaagac aacgactgat tttcgcacat catcttgatc gattctccgt ccaaaaatat
     6181 tgatgttatc ttcacttttg aggaaggaat atagatacaa accgatgatt gctacagtag
     6241 ttgttcgaat cccgccgcct acagaactag gactgcatcc gataaacatc agtaaagaaa
     6301 aaatgatcag tgttgtgatt tgaaaatcac ccaaatcgtg aatctgcaga ccagcattac
     6361 gagtcgtaat cgaataaaac atcgaattga tccatttgac actcatattg gaatcttgga
     6421 ataaatggtc cttttccaat agataaatca agacagttcc cgaaacaaac aagataacaa
     6481 aagctaacac agctaatttg gtaaacaagg aaaaacgaaa cggaagcttc gcattggaac
     6541 gtttgtataa tagccattcc cggcactcca tcaatacagg aaaaccaatt cctccgacaa
     6601 aaatcaaaaa catgatcagg atcaagaaaa aatagtcatg tgcaaagggc ttgatggaat
     6661 ccccagtgac gtcaaaccca gagttcgtga cagccgatat cgcctgataa aaaccgtaaa
     6721 aaacggcatc acgccaacga tcaaaatagc ctctgtaata aaaataaata gaaaagaatg
     6781 tcccaaaaag taattgaaac cagattaaaa tagcaaaagt gatccgaatc agtcggacga
     6841 taccagacaa acgcggttga ttcatatccg tcatgatcag ctgtcgctgt ttcagtgtga
     6901 tccgtcgttt agaaaaaatg acaaaagcag tagaaatcat catgatcccc aaaccaccaa
     6961 cttgaaataa tatttccagc agtataatac cgttatcatt gaacacagaa ttgatatcaa
     7021 aagtcgatag tccagtcaca ctcactgtac tgattgccat gaacagcatg tccacaaaag
     7081 aaacatggct atctggttcg cgaaaaatcg gcatataaaa tagaacgagt gataagaccg
     7141 tcatcaatat ataataaaaa acgatgattt ggatcgatga gaaatgattg gcagcaaact
     7201 tctgtcctcg ccgctgcagc cttcgaaaga ataaatcaac atgcacagta ctatcctcct
     7261 gacaatagtc gtttctacca gtatacaagg ggaattagga atactcaaag aaattatcaa
     7321 acagataaca atacagcttt cgattaaaat agaaatggct tataataagg aaaatcctag
     7381 aaaaaagaag gaggaggaat aacaaattga ttaatgctca acaaatcgta gaaaaaatga
     7441 aaaatcaaaa aataaattat gatacagttc tgaaaaaaat gatcatgcaa tgggagaaag
     7501 aagaagtaaa gcctaagata ttaatacata catgctgtgc tccttgcagt acgcacacat
     7561 tggaattcat gtctcaatat gcagagatca cgttattttt ttcaaattcg aatattcatc
     7621 ctaaaagtga gtatttgcga agattacatg aacaaaaacg atttgtagac caatttaatg
     7681 agaaaactga ccatcatgtt gcgctaatcg ttgatgaata ccgtccaaat gaattcacca
     7741 aaatggtttt aacaaaccat cttgaaaacg aaaaagaagg tggtgctcgc tgcagtgcat
     7801 gcttcaatat gcgattagat cttgttgcgc aaaaagcgca agaactagga tacgattact
     7861 ttggaagtgc cctgaccatc tcgccaaaga aaaatgcagc tttgatcaat cagatcggaa
     7921 tggatatcca aaagatttac gggacacaat acttgccaag tgatttcaag aaaaacaaag
     7981 gctatgaacg ctctttgcaa atgtgcaaag agtatgatat ttatcgccaa tgttattgtg
     8041 gttgcgtgtt cgctgccaag cagcaaggat gcgatctgaa agaaatcaat cgggaagcaa
     8101 aagcttactt aaaaacatca accgtagttt ttgaggaaaa ttaaagaaat ctttcggaaa
     8161 aacgtaagaa aaaaataaag aatttatggt agaaataagg tagactgtat aatttgtccg
     8221 tagtaaagta aggaaacaag aataaatttc aatgaaggaa gagataagat gcgaaaagta
     8281 gcacctattt tgaagccaat cgctgtatta acaatctcga cgattgtcgc gactactaca
     8341 ggtattgtga atagttccag ctaccgtgca ccaagttcaa gttctagtac tgtagaagaa
     8401 acaacaccaa ctacttcagt ctcgacatct gaaccaacaa caacggaaag tttgaatgaa
     8461 accactacga caagtacatg ggacgataca acaactagtt cgtcgtatga tgagacatat
     8521 acaactgaca gctcaagcga aacaagtaat acggtaccag atggcactcc atcggattca
     8581 accaatccaa gtacaagtcc ggatacaagt gctagctccg aaacgatacc aagcagtacg
     8641 actgtagatg ataacacgca aaatgatgat gcagcacgta cagcacagca aaaccaagat
     8701 tcaggaaata cccaaacgta taaccagcaa gcaggtgatt ctcaatgaca tttttactag
     8761 accttatcaa cggggtgcat aaattccttg gctatttaga catcagtcct aaatatctta
     8821 accgtggata tacgatttta agtgtgattc ccacgcttta tttattgaga attgtttatg
     8881 gactttggca aaaccaaaac tatcttcaat ttttcttata tggacttgca tttctagtgc
     8941 ttgtttattt tactgtttta aatgtatttt attacttttt agataaaaac acgaaagccg
     9001 atgtcactca gctttttgtg aagtatttac ctgatgaagc gttcaacatc cagacagaag
     9061 aatcgataga taatggaaat tcgatcgata aagtgaatac acaagaggtc atggtttctt
     9121 atgacgaaga ttatcaattg aaattagccg agaatatgcg gtatttgatt ggaaatggtg
     9181 agatcaaaac aaatgaactc ggtcgcatgg atggatttct ggtcgataga aatacattgt
     9241 atccttatta ctatgtcaag aaaacaggag agaaagagta tgttctaaat attggacgaa
     9301 gctactcgga catggaaaaa gtagggacca ttcgacttga ctcaacagaa gacacattgg
     9361 aacctgtcgg attgttcatc gtcggcggtg attttgtcaa agacgggtta cgctatcatg
     9421 agccttatcg tctgaaattg attgcaaaaa aggcacagtc gcaggaagaa ccagtggttt
     9481 atagtcgtag ccaaagaaga agacattcat aatgtgatga aacgccgtgt atcagtggtt
     9541 gcgataaaaa cgtttattcg gaataagggg gatgtgacac gtcttttgtc acgttccttt
     9601 tttatataac gagaaagata agacgaacac atcacttatt ctttcctttc atttataaac
     9661 attgtagaat aaagaaagta caacgaagta ttgtgaaaaa acgtaataaa taacgaggaa
     9721 aatacaagta ggaggtaagc tgtgaaagaa tttttgaaaa attgggggat tttgatcctt
     9781 gtattagcgg ctattttatt agctagagtg tacgtgttta caccagttac tgtaaacggg
     9841 cattcgatgg accctacatt aagtgatgga caacgattga tttcatctaa gatctcaaac
     9901 tatgaacgaa tggatattat tactacaaag gaaccggggg atgaagaacg gatgattgtt
     9961 aaacggatca tcggtatgcc tggagataca gtcaagatgg aaaatgacca gttgaccatt
    10021 aatggcaaaa agtatgatga accttatctg gatgagttca aaaaagaatt ttcagaagat
    10081 aagttacaag gagaatatgc ttatagctca ggattccaag cacaagcaga aagttcatcc
    10141 acctttacga gtgactttga atatactgtg ccaaaaggaa agtatcttgt tctaggcgat
    10201 aaccgcttga tttcaaaaga cagccgaatg tttgggttag tcgacaaaga tatgatccaa
    10261 ggaaaagttg ttttccgtta ttggcctctt tcagaaatta aaatcatgtg atgaaaaaat
    10321 aaaaagaagc tgtgacatgt tttttgtcac agcttctttt tccgaaaatt ccttcttatt
    10381 tcttggagtt gctcactttt tttgcaatct cattttaagc tttttggctg atggataaga
    10441 aaagcgaagc gtttttgttc ataaatagct tttccccacg atggaaggta tgactagtag
    10501 cagagacaaa gcccgttttt tccaatcgat tattcaattc ttcttggcta aagccattat
    10561 ggactttcgg atgactaatt tgttcattct tgtcaaagtc tacgatgatc aattgaccgc
    10621 caggagctaa aatttcataa agttttgtaa gaatattctc cgtatcagga atgtgtaaaa
    10681 gtacaagtga tagaaggatg agattagctt ggacagcagg aaattcgaca gaagcatcag
    10741 cgtggatcgt tcggacattt ttcagatctg ctgcttggat cttttcttct gccattttca
    10801 gcatagtttc tgaagcatcg gcaatgatca gttctttgaa tctttccgca agcggtaaac
    10861 taacaagccc agttccgcct ccgtaatcca gcaggacttt ttggtggctg tcgcgcggta
    10921 gataagtagt tagttcttcg cgaataatct tggctaatgc ttgacggtca ggggaatcat
    10981 aatggttagc gatggaatca aaaatatttt ttgtcatcaa atagctcctt acgtaaaact
    11041 ttgaaatttt aacttattat agcacgtgaa aagaaagaaa gcatgctata ctcaattcaa
    11101 aaagggagaa tctagaaaga atatgccgat atcgtttgta aaagatagag aggaaaaagg
    11161 aaagtgtgtc cgagaaatat tactagactt gcctgagtgg tttgggcttc ctgaatcaac
    11221 ggaaaaatat atcgaggaat caagtaaact acctttatgg tgtgaaaaaa gaaaagaaga
    11281 atatttaggc tttatcactc tttcacagac aagtgaagat actgccgaaa tctattctat
    11341 tgtatgggaa taaaaaaaga ctatcatcac caaggattag gaacaaaact ttttaaagaa
    11401 gcagaaggtt acgcagcaaa acattacaaa tatttgcaag tgaagactgt agacgagggg
    11461 cactactcta tatatgatca aactatttgt ttctacgaat cattaggatt ttcaaggttg
    11521 gaagtttttc caaatttatg ggatgaatgg aacccatgtt tagttcttgt caaaaaattg
    11581 gaacaaaagt gagcaataga tttaatacta atctgtgctt gatgaaaatt ctcactgtaa
    11641 aaaatgctga aaatcaagta taataccata ggaacaaact ggaaggtgaa aaagagcatg
    11701 gcacaactat tttttaagta tggcgcaatg aatagcggca agacgatcga gatcctaaaa
    11761 gtcgcacata attatgaaga acaaaataag ccagttgtct taatgacaag cggtattgat
    11821 acaagagatg gtttaggcgt tgtttcaagc cgaatcggac tgaaacgtaa tgcgatgccg
    11881 atatttgaag aaacagacgt atttgacgta attcagaaaa tggaaagcaa accctattgt
    11941 gtgctgatcg atgaagcgca attcttaaaa aagaaacatg tcattgcttt tacgagaatt
    12001 gtggacgaat tggatattcc ggttatggca tttggtttga aaaatgattt tcgaaacgag
    12061 ttatttgaag gatcaaaata tttattgctt tatgcagaca aaatcgaaga aatgaaaaca
    12121 atctgttggt tctgccacaa aaaagcaatc atgaatttac attatattga tggaaaccct
    12181 gtttatgaag gcgatcaagt gcaaattggc ggaaatgaag cttattaccc cgtttgtcga
    12241 aaacattact ttcacccatc aatgattaca gaaggagaat aagcatgtac gatcaattac
    12301 aagcaattga agaccgttat gaagagcttg gagaactttt aagtgaccca gaagtgattt
    12361 ctgatacaaa gcgttttatg cagctttcaa aagaagaagc aaacacgcgc gaaacagtcg
    12421 aagtttatcg tcgttataaa gaagtagtac aaggaatcac cgatacagaa gaactgcttg
    12481 gtgaaaaact ggatgatgaa atggctgaac tagcaaaaga agaattatct gaattaaaaa
    12541 aagaaaaaga agtcttagaa gaaaaaatca agatcttgct tttaccaaaa gatccaaatg
    12601 acgataaaaa tatcatcatg gaaatccgtg gagcagcagg cggagacgaa gcagcattat
    12661 tcgctggcga tcttttcaat atgtaccaaa agtatgctga agcacaaggc tggaaaacag
    12721 aagtgatgga agctaacgtt accggaatcg gtggatataa agaagtgatc atgatgatca
    12781 ccggagacaa cgtatattcg aaattaaaat atgaaagtgg tgcgcaccgc gtccagcgtg
    12841 ttccttcaac tgaatcgcaa gggcgtatcc atacttccac cgcaacagtt gtcgtgatgc
    12901 cagaagctga agaagtagag atcgatattg aagacaaaga tatccgtaca gatatctatc
    12961 atgcttcagg tgctggcgga cagcacgtca ataaaacagc atctgctgta cgtttgactc
    13021 acttaccaac agggatcgtt gttgctatgc aagatgaacg ttctcaaatc aagaaccgtg
    13081 aaaaagcaat gaaaatcttg cgtgctcgtg tgtacgataa gatccagcaa gaagcacaaa
    13141 gcgagtatga tgccaatcga aaatcagcag tcggaactgg ggatcgttca gaacggatcc
    13201 ggacgtataa tttcccgcaa aatcgtgtga ctgatcatcg tatcggatta actatccaaa
    13261 aattagatca gattttagca gggaaattag atgagatcgt tgatgcattg atcatgtatg
    13321 atcaaacatc aaaactggaa gctatgcaaa atgggtaaaa tgacctatcg agaagtcctg
    13381 tcacgggctt cttctttttt agaggatcat gggaaagaag catatagtat ccaattttta
    13441 tttttggaac gaaaacaatg gaaaaaactt gattggctcc ttcatatgaa cgaagagatt
    13501 tcagaagaag aacagcggtt gatcgaaacc gatctgcgac ttctgcttgc agatcatcca
    13561 cctcagtatt tgctaggata tgcagatttt tatgatcatc ggttaaaggt aactgaagca
    13621 acactgatcc ctcgaccaga aacagaggag ttagtcgaat ggtgtttaga cgagacgcct
    13681 ggtgtaccgt tagaagtgat agatattggg acagggactg gagcaattgc tattagtttg
    13741 aaagcagcaa gaaaaaattg gcatatatct gctgtggatc tttcagaaga agcattagaa
    13801 gtagcgaaag aaaatgctca gaaagaagga acgaaaatca gtttttatca tggagataca
    13861 ttaggaccag taatggatca gcaatttgat gtgattattt ctaatccgcc gtacatcagt
    13921 cggaatgaat gggaactgat ggatgaaagt gtccggtcat tcgaacctaa aatggccctt
    13981 tttgctgaga acgatggatt agctatttat gaaaaaatag cagttgaagc accaagcgta
    14041 ctaaaatcaa atggaaagat ttttttagaa atcggatttc gccaaggtga agcagtaaaa
    14101 aaaatatttc agcaagcatt tccagataaa aaagtggcga taaaaaagga tctttttggc
    14161 aatgaacgaa tgatccaagt ttatggttga agttgtggat gaaaaatttt ttatttgttt
    14221 ttttattata aaaaaacact tatttgacaa ggatgtaaac gactttttgg tttatcaaca
    14281 agtttatcca ctattgacaa atagattatc cacaaacagt ggaaaacttg aggataaact
    14341 cagacaaaat aagaatgtat gaaaaagggg ttgtggataa actgtggaaa cgaaacgata
    14401 tgatgaaaca gaattgcaag aggcagccga agcactgaaa gaaggagaac ttgttgcttt
    14461 tcctaccgaa acagtatacg gattgggcgc caacgctctt ttaccaaacg ctgtcaagaa
    14521 agtgttttct gtaaaaggga gaccgcaaga taatccatta atcgtccatg tcgcctcttt
    14581 tgaacaagta aaagagtatg tggacaactt tcatccggca gcagagaaga ttgtgaagaa
    14641 cttctggcct ggaccattaa ctttgatatt taagatcaaa aaagacactt tgccctcagt
    14701 tgtgacggga ggattatcca cagctgcatt tcgtataccg gataacaaaa aaacactaga
    14761 agtaatcgaa ctttccggtg ttccgcttgt cggtccaagt gcaaatacgt cgggaaaacc
    14821 aagtccaacg actgcagatc atgtatatca tgatctacaa ggaaaaatta ccgggattat
    14881 tgatgatgga gctacgcgaa ttggtgtaga atccactgtt cttgatttaa gtgatccaac
    14941 agccatgcct atgattttac gaccaggtgc agtaacgaaa gaacaaatcg aagcagtcat
    15001 cgaatctcct gttgctattg atcagcatct tgtaaaagaa aatgaaacac caaaagcccc
    15061 tgggatgaaa tacaaacact actcacccga tacacgtgtt ctgatggtca gagatggcga
    15121 ttggtcaact gcagttcaat gggcaaaaaa taagaagatc cgtgcaggtg tcatagcaag
    15181 ccccgaaatt gcggatcaag tgcggacaga tactgcagct gtttatatgt ataacgacaa
    15241 ctcagtagaa gcggcggcta aaggattgtt tgcaggtcta agaggattag atgaaccaac
    15301 acttggatta gatttgattt ttgtacaagt gtatccagaa actggattgg ggaatgctta
    15361 catgaatcgc ttgaaaaaag cagcaggaca aaattatttt gaaaaatgat caattggtta
    15421 ttgtcgcatt cttttagtat gatacaatgc tatcaagtaa atcttctatc agtgaacgaa
    15481 aaactaagcg taactaatcg taatagaaga gatacgaata tttggcaatt tgtttttgat
    15541 gggaaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag attacaaaac
    15601 gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag aacataattt
    15661 ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac aaggaagtat
    15721 cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg ggtgtgaatt
    15781 tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg gagcgaaatt
    15841 tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc tcgctttggt
    15901 agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc taacacatgg
    15961 atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg ttgatccgac
    16021 aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc aaccaaaatt
    16081 gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat tcagagaaat
    16141 cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag gattagtagc
    16201 agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta ccactcataa
    16261 aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat tagcaaaaaa
    16321 aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg tgattgcggg
    16381 taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata gtgaacaaat
    16441 catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa ccagagtcat
    16501 ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag gtataaatgg
    16561 aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa actcgattcc
    16621 ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc cagcaatcac
    16681 tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg tcaaagcatt
    16741 acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac gcgaattaac
    16801 tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa cagaaaagtt
    16861 caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa tatcggtgat
    16921 gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg aggaaaagtc
    16981 atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc aaatgcgcct
    17041 catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga agaagtacaa
    17101 gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga tactggcgaa
    17161 tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa attaccagtt
    17221 attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca tgataaattg
    17281 atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata tagtcgatca
    17341 ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca acagctgaat
    17401 aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg agattcagta
    17461 ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg cctcccaata
    17521 tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga ttcctatcat
    17581 gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg ggaccaatat
    17641 acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc aaatataaat
    17701 gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt attaagagat
    17761 gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac acaagtacgc
    17821 ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac aaaggcaacg
    17881 cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc tttgcttgtc
    17941 cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag tttgactagt
    18001 caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga gagcttaaaa
    18061 aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa ctattattcg
    18121 ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg ctatgttgat
    18181 ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa caccgatcac
    18241 tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa tcttgcgcgc
    18301 aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag tgggacatat
    18361 cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca aacttccaga
    18421 agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag gcggatcagc
    18481 aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat ttgtttgttt
    18541 ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata ttgacatcta
    18601 tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg gactagggga
    18661 tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg tgacacaact
    18721 tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt tttcacagct
    18781 ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt caaatctttt
    18841 ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag ttcgcatttt
    18901 ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa atcgttccag
    18961 tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat ttttcaccaa
    19021 tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc ataaaacctg
    19081 cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa ctaattttcc
    19141 atcggactct ttacaatcag ccaaatagag tatataattt cttttggata aggccagctt
    19201 agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga tccctatagc
    19261 tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata catctgtctt
    19321 tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta tttctataaa
    19381 aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca catgaactgc
    19441 ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca atcttgccac
    19501 cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat cataagtact
    19561 gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa tgctgaactt
    19621 actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta tcatgatagg
    19681 caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg ttaaacccca
    19741 tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat tgataaaaaa
    19801 atatttggct atatttttga caggagcgac aggtttcgag aacatttttt tcgtatcttt
    19861 tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa aaatgctaat
    19921 aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag tgcccaatat
    19981 ccctttttta agttctattt tttttcctaa aatgttattc atgataataa ctcctactat
    20041 tcagtttttg aagatagagt cagtatacat gaagagttaa aataggcaat gtttttttat
    20101 ggatatttgt attgctaaaa atttatctta tatattcatt ttgattatac tacttttatt
    20161 tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat taagcgtaca attttttaga
    20221 tgttccgagc gtacttcaat catttcaaag gagttgctac ttaaatcgat attttttaaa
    20281 tcagtatcaa aaaagctagc attttctaaa tcattagtag aaaacattat tttagtgagg
    20341 gaacagtcta caaaatctgc gcttgataag acagagtgct caaaggtact ttcttttatg
    20401 tttgaaccag ataaatttac atagtccatt ttacaattct cgaataacgt gttcccaata
    20461 taactttcca tcaatttagt acctgttaat ttacaattga caaatttcac gcgatgcaac
    20521 atgctttttg tcaatataca gcctgaaaaa tcacagtttt cgaatataca gtcggtaatc
    20581 tccattcttt gaaaatcatt ggaagcaaaa aatgcatact tgaaaataga tttgtaaata
    20641 attggatggc agattatttg gttttcgatg acctcttttt gtgaaaggat attatacagt
    20701 tcaaaatctt cgttttcata gatatgaaaa aaggatcctt tattcaagct tgcttcatta
    20761 actttaggaa aaatcatatt taattacctc cattttcata aagtcatatt aacaaaagct
    20821 aaaccaactt acattagcag aaaatgagat aggttttcgc ctttttcgat aacgatagtg
    20881 tcgctatctc ttgaaacgtt cttagaattg ccctgagaga cacaaattag gcgtaattct
    20941 aataaaaatc ttagaataaa aatttttact gatttaattt agaatgtttt taaaataaaa
    21001 atcgttgcca taacagtgta ttgaaaggtt ttaacaatcc agtaatgatt taaaaaaatc
    21061 tagatgtagc gtatactttg tttatagaat gtagtgggaa gaaatatatt tttgggagga
    21121 aatcgtgatg tctaaaagtg aagaaataga aaacatggta ctaaaattat atgatctcgt
    21181 tttaaatcca gagatcaaag aaaaggaacg tatacttttg atagacgcaa agacaggctt
    21241 agaaaaaggt cagtattatc ctaaagtcat aaataatttg gaacgtagtt tacgtccgtt
    21301 agcaatcagg ggagaattgt ctaaaccagt atcaccgttt tatatggaga tatcaacaat
    21361 aggaaaattt gaaaaagaat taggtcgagg aatggcttcg gcaccgatta cttttgggca
    21421 tttataaaaa cgagttattc atctcaatga attaaaaagt atagtcaatc tcataatgca
    21481 ggctaggttc atttatttga acagagtctg tattttttta ttctaaataa agaaacagat
    21541 gtttctttat attgtatttt atcct
//
LOCUS       DAXWLG010000032        21405 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXWLG010000032 DAXWLG010000000
VERSION     DAXWLG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.032"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            226..1395
                     /locus_tag="ACGE4T_000969"
     CDS             226..1395
                     /locus_tag="ACGE4T_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFF8386270.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1868..2422
                     /locus_tag="ACGE4T_000970"
     CDS             1868..2422
                     /locus_tag="ACGE4T_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8386271.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2595..3068)
                     /locus_tag="ACGE4T_000971"
     CDS             complement(2595..3068)
                     /locus_tag="ACGE4T_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8386272.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3080..4027
                     /locus_tag="ACGE4T_000972"
                     /pseudo
     CDS             3080..4027
                     /locus_tag="ACGE4T_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4298..4733
                     /locus_tag="ACGE4T_000973"
                     /pseudo
     CDS             4298..4733
                     /locus_tag="ACGE4T_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4749..5582
                     /locus_tag="ACGE4T_000974"
     CDS             4749..5582
                     /locus_tag="ACGE4T_000974"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8386273.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5827..6852
                     /locus_tag="ACGE4T_000975"
     CDS             5827..6852
                     /locus_tag="ACGE4T_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8386274.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            6990..7115
                     /locus_tag="ACGE4T_000976"
     CDS             6990..7115
                     /locus_tag="ACGE4T_000976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386275.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7140..8255
                     /locus_tag="ACGE4T_000977"
                     /pseudo
     CDS             7140..8255
                     /locus_tag="ACGE4T_000977"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8338..9234
                     /locus_tag="ACGE4T_000978"
                     /pseudo
     CDS             8338..9234
                     /locus_tag="ACGE4T_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9250..10074
                     /locus_tag="ACGE4T_000979"
     CDS             9250..10074
                     /locus_tag="ACGE4T_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386276.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10106..11416
                     /locus_tag="ACGE4T_000980"
     CDS             10106..11416
                     /locus_tag="ACGE4T_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFF8386277.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11564..12823
                     /locus_tag="ACGE4T_000981"
     CDS             11564..12823
                     /locus_tag="ACGE4T_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8386278.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12833..13705
                     /locus_tag="ACGE4T_000982"
     CDS             12833..13705
                     /locus_tag="ACGE4T_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386279.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13717..14547
                     /locus_tag="ACGE4T_000983"
     CDS             13717..14547
                     /locus_tag="ACGE4T_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386280.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14587..16770
                     /locus_tag="ACGE4T_000984"
     CDS             14587..16770
                     /locus_tag="ACGE4T_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8386281.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16831..18051
                     /locus_tag="ACGE4T_000985"
     CDS             16831..18051
                     /locus_tag="ACGE4T_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFF8386282.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18048..19508
                     /gene="gtfA"
                     /locus_tag="ACGE4T_000986"
     CDS             18048..19508
                     /gene="gtfA"
                     /locus_tag="ACGE4T_000986"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFF8386283.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19585..20691
                     /locus_tag="ACGE4T_000987"
                     /pseudo
     CDS             19585..20691
                     /locus_tag="ACGE4T_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21013..21222
                     /locus_tag="ACGE4T_000988"
     CDS             21013..21222
                     /locus_tag="ACGE4T_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFF8386284.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21215..21364
                     /locus_tag="ACGE4T_000989"
     CDS             21215..21364
                     /locus_tag="ACGE4T_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFF8386285.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT         7356 a         3208 c         4110 g         6731 t
ORIGIN      
        1 tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac aatattaatg
       61 aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag acaaaatgat
      121 agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag tccattctgt
      181 aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc caattttact
      241 acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt tcgatgtcat
      301 ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct tgattatgaa
      361 aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta ttcacgttca
      421 ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa tggtaagttt
      481 tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt
      541 cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga aaaaatgtac
      601 gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc tgaagatatt
      661 attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat ggatgctact
      721 cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt aattggcatt
      781 cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga atctgcttat
      841 gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt tttattagtc
      901 gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc aaatgctcaa
      961 tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat
     1021 cgacaagaaa tctgtagcga tttcaaatta gtttatcaag caagttcaaa agaagaagca
     1081 aataatcaaa taaggtttat gatagataaa tggaaaaagc agtatccacg agtggttaaa
     1141 ttgctcatga atcctgctat attaaccttt tataatttcc ctccgtcaat cagaagaact
     1201 atttattcaa ctaatttgat tgaaggtttt aacaaacaat taaagaaata taccaaaaga
     1261 aaagagcaat ttcctaatga agaatctctg gagagatttc tagtatctca attcaacaac
     1321 tataatcaaa agtttctatg ccgagttcat aaaggtttta aagaaataca tgatacatta
     1381 gaatcaatgt tttaacttga tcaacagaat gggcttttcc atttacacaa aattattgac
     1441 gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa atattttatt
     1501 aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt atagtaaggg
     1561 gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt aaattagcta
     1621 ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct tagaatcttc
     1681 atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt tactgaaatg
     1741 atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt atatatataa
     1801 gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt taagaaggga
     1861 aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac aagattttca
     1921 gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt tgtttggaat
     1981 caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata tacgaggcaa
     2041 aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt ttatagataa
     2101 tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt ggttatttcc
     2161 gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat atgcagatat
     2221 agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa cttttgggta
     2281 tcactactac aagaaaacta gagacattgc taccttaatg tttattttta atcatagctc
     2341 gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag catcacttcg
     2401 tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac aaagattaaa
     2461 agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa gctggggtta
     2521 ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca tttttaggtt
     2581 ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca aacgctgcgg
     2641 aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga tagtctgctt
     2701 ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc ctctgtagaa
     2761 ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc gatatgggtg
     2821 tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata cacttctttg
     2881 acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc atgaagtgtg
     2941 atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac atagtctaaa
     3001 taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt tataggtttg
     3061 attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg tgaatcgctt
     3121 tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac taatgaaaac
     3181 aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc accaccgtgt
     3241 gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt ttggaaccaa
     3301 tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt tttgggaaat
     3361 atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg gacgacctat
     3421 cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac gcgaatttga
     3481 agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc atgccatttt
     3541 aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac gagagttatt
     3601 gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc aacgagtgac
     3661 aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc aagatcgtga
     3721 gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg gcgtagaaaa
     3781 taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta taaattcagg
     3841 aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc aattattgat
     3901 tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc aaagtccacg
     3961 ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg aacaagtgga
     4021 gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat aagtcaaaaa
     4081 ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg ttcctatttt
     4141 tataaactat cattttcgac taataaaact acttatgagt cagcctctct ttttttcttg
     4201 atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg tctcattgaa
     4261 acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata ttgaaattat
     4321 ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat aaaattagta
     4381 tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact attacccaag
     4441 aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt ttacctaaat
     4501 acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg gtcaatcaaa
     4561 agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc aatgcgttat
     4621 ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat gcttattcat
     4681 ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg attgcatttt
     4741 taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt tctgatccat
     4801 ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt tattttgtat
     4861 tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat tggcgttttg
     4921 aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat aacgtttttg
     4981 atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga gaacttgaac
     5041 aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca ttggcagtgg
     5101 aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac tacattgtag
     5161 gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta aagaaatatc
     5221 atttaacctt taaatcgcat tggatatata acaactctga atttgagcga atttatcaag
     5281 aattagctga atttactgca attgttgtta ctggagatta tttagcggct aaattagtaa
     5341 aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt ggatttgatg
     5401 atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta aatccaggta
     5461 acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa gaaataaaaa
     5521 atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac aaaatcaaat
     5581 aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat agagagaatg
     5641 aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat ttactctaaa
     5701 ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt taacgaaatt
     5761 tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat gaaaagagga
     5821 gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat tgcaacagta
     5881 tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa acgaaagatt
     5941 gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa aaatttaaca
     6001 aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc ctattttcga
     6061 acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt gaagaaaatt
     6121 attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca aggtgtgttg
     6181 attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa ttcgaatgtc
     6241 gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac tgatttaaca
     6301 aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa cattgcgttt
     6361 atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc agatgatgat
     6421 tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa atatagtcaa
     6481 atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt acttttgaaa
     6541 aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc tattgctgtg
     6601 ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat ttcgattgtg
     6661 agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac ggtgaatgtg
     6721 aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat aaaagaagtt
     6781 agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga aagtgaaaaa
     6841 aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt ttactaatat
     6901 ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt taacaaaaag
     6961 aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata gctttaacag
     7021 ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat agtaattcta
     7081 aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct ccaggactaa
     7141 gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa atggagacaa
     7201 cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt aatatgccag
     7261 atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat gctttgatgg
     7321 gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact gaaggaattg
     7381 ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct tctgtagtgg
     7441 ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca gatgaaacat
     7501 ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca aaaggcatct
     7561 atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt tatcaaaatg
     7621 ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca gaagcacaag
     7681 aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca acaattacag
     7741 aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca atgatgattg
     7801 aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa aatgttgctt
     7861 ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt ggttgggctg
     7921 ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt ttagctagtg
     7981 aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta aaaggctttg
     8041 aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga aaaatgttgg
     8101 agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa gcagaagttc
     8161 aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc gctcaaattg
     8221 aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac aatatacagg
     8281 cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg ggatattatg
     8341 aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta tatttttatt
     8401 tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca aacagcaatt
     8461 ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt aagaaattac
     8521 caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt aatttatgca
     8581 gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt aatgaataga
     8641 aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt tgctgcacca
     8701 tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact gattaataac
     8761 atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc gattgcgatt
     8821 tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt gttattacta
     8881 gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga tggtgctagt
     8941 ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt attttttgta
     9001 gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat gatgattact
     9061 cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta taaacattca
     9121 tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt agtgattata
     9181 ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta ttaaggaggc
     9241 tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat gcttatttta
     9301 ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg gaaaacgttg
     9361 gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct aagtaactat
     9421 caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt tttaatggtt
     9481 gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc ttgtgcgcgt
     9541 ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat gatgattccc
     9601 ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat tttaaataca
     9661 attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt attaagacaa
     9721 tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg tgccaatatt
     9781 tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt tgcacttgga
     9841 atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt caatacttca
     9901 attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata tgccacgaac
     9961 ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt gatattcatc
    10021 gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt ataaaactat
    10081 taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg caggaagtac
    10141 tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac aagatgcaca
    10201 tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa tgcttaaaaa
    10261 tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg atcgcaaaga
    10321 agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt ataaaccttc
    10381 aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta ttggtgacac
    10441 catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt tagactttgc
    10501 taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa atccgatggc
    10561 gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt tatgtcatag
    10621 cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc aatatgactt
    10681 aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt tggaaattac
    10741 aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa aaccaaatcc
    10801 acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata ttacagaatc
    10861 aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc cagaattgat
    10921 tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac aaattaatga
    10981 ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata cgagaagccg
    11041 tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag tgattgcagg
    11101 aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg tggaagtacc
    11161 ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc caacacaatt
    11221 agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag ccgctttaac
    11281 actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat catctgaatt
    11341 gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg gggactattt
    11401 acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc ttactctttt
    11461 cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac attagtgtta
    11521 acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt ggtctaaaaa
    11581 aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt gtggaaaaaa
    11641 tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa aagaaatgac
    11701 tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata ttcatgtaaa
    11761 agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg caggcgatgt
    11821 accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg cgaaagctgg
    11881 ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg gatatgcaca
    11941 acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg tctatggttt
    12001 ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa cttgggcaga
    12061 atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg cgattgcagg
    12121 agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg ctgctggtgg
    12181 tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta atgctgaaag
    12241 caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa aagctttgca
    12301 aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa aaggtgatgc
    12361 gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa atccagattt
    12421 tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa cgattggcgc
    12481 aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag ccaaacgttt
    12541 tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag atggtgcacc
    12601 gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg gtttaactga
    12661 acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata gtgaaaatga
    12721 tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca tgattaacgc
    12781 attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata aaatgaaaat
    12841 aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa ttattttgca
    12901 gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa ctaattggac
    12961 tggtctgact tataattttg attttgtggg tttgaacaat tataagattc ttttttctga
    13021 tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca tgattgctgg
    13081 acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta aagggaaaac
    13141 attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg tttctttagt
    13201 atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag gcattgaatt
    13261 tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa tttttgtttt
    13321 actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc aaagtattcc
    13381 acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa ttttttggaa
    13441 tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg cattaaaagg
    13501 tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa gcgatgccac
    13561 aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt ttggttatgc
    13621 gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg cacaattaaa
    13681 attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa agaaaaaaat
    13741 atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc cttagtttca
    13801 acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt tcgttttcct
    13861 aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg tcattacttt
    13921 tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat tattcctatg
    13981 gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt gatgtattta
    14041 tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat taccgttatg
    14101 atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct cacttatgcc
    14161 gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc tatcagtctg
    14221 gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa aattgttttc
    14281 ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg gttttggaat
    14341 gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac actaccattg
    14401 tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt tgcttcctat
    14461 acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag tattatagct
    14521 ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa aggagttcat
    14581 aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa taaaaaaatt
    14641 agctatctat taggaatcga agaaaataat tatttaacgc atctttattt tggaaaaaag
    14701 attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt ctcacccaac
    14761 aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga atttccagga
    14821 tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa cggtagtcgt
    14881 gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag cttagcggga
    14941 ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat tcaattgaaa
    15001 gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa tcacagtgta
    15061 attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat taaccgctta
    15121 gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt aaatggtacc
    15181 tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa agtattagat
    15241 agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt agatgccaaa
    15301 acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg taatcacgaa
    15361 acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat taattcattt
    15421 aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt cgtgatggtt
    15481 tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta taatcaccgt
    15541 ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa ctgggaagca
    15601 acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc gaagaacttg
    15661 ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa tgacttcact
    15721 tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga aaatttagcc
    15781 aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga aatgatttct
    15841 gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc aggtcgtgac
    15901 cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt tcgtgataat
    15961 atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt gaagtgggat
    16021 atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat gcaaggtgaa
    16081 gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac aacgaattat
    16141 ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc aggtttcttg
    16201 tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg tctgaaaatt
    16261 caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt ttcagcagtc
    16321 ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc tgcgatggct
    16381 ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa agcagaaatt
    16441 aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg aactttttat
    16501 cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc aaaagaccaa
    16561 acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc attagtcaca
    16621 ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt aacatttgct
    16681 ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg tgattatgcg
    16741 acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata agtgcaacac
    16801 caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt tactaaaaag
    16861 cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc aatgattaac
    16921 tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa catcaaaaaa
    16981 aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt cattgggccc
    17041 tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga gcggacattt
    17101 aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg tttagatgaa
    17161 tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac ggatattgat
    17221 gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga tatgcgtacc
    17281 aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt atatttagaa
    17341 aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat caatgattat
    17401 agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt tatttatcaa
    17461 ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt tgatgatgta
    17521 gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga gcaagcattg
    17581 aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg gaatacgcaa
    17641 aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc taattatcca
    17701 gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta ttatcatcaa
    17761 ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg acaatttcta
    17821 ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga agataaaatc
    17881 gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga tgaacagtac
    17941 caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg aaaattgcgt
    18001 ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg aaaattaaaa
    18061 acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc gaattaaaac
    18121 aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg cctttttttc
    18181 catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat ccagctcttg
    18241 gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt gattttatga
    18301 ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac catgagcagt
    18361 caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat cgaccaacac
    18421 aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa acggtagaat
    18481 ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa attgatttag
    18541 atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat atggcgaacc
    18601 atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa ttagatacca
    18661 atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt caagaagcag
    18721 cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt caaatgaaaa
    18781 ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact ctgtattcac
    18841 tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg atgaagcagt
    18901 ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat ttattaacag
    18961 atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat gtgaagaagg
    19021 tctattcaag tgccaattac aataatttag atatttatca aattaacagc acctattata
    19081 gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa gtatttgcgc
    19141 cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat attgaactgt
    19201 tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta gccgaaattg
    19261 aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa tttagaaatg
    19321 aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca gaatcagaaa
    19381 tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg gctaacttgg
    19441 atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt gttgcattaa
    19501 aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga tgacttatcc
    19561 aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc atttaataaa
    19621 tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg tatttttgat
    19681 tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt tctctacgca
    19741 gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga ccgatcgctc
    19801 caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc atttaatgca
    19861 cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt tcaacatcaa
    19921 atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt ttcgcttagt
    19981 ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa gttacactta
    20041 aactattgtt atatggacga tactacgatg tatccaactg attttacctt aacaaatgcc
    20101 catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc aacttacgtg
    20161 tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga tggctatttc
    20221 ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct ggaaacatcg
    20281 ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac ctatctaacc
    20341 gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca atttatcacc
    20401 aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg ttggcaaatc
    20461 gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt ttctcaaagt
    20521 gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct cacgtttctc
    20581 atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt ctttcgttat
    20641 ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg agaaaaaaga
    20701 aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt tctggaaaaa
    20761 gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat agttttcaga
    20821 atattctaaa atgttttatg caacactagt gatatttttt agaattagat ttctaatgtg
    20881 cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt aaatttgaat
    20941 aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt gaataaaaaa
    21001 tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg ttatgggaag
    21061 tatcagtctg agagaatcat acgacaggca aaagcaatta tggcttcaaa aagctatacc
    21121 tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga agttttaggt
    21181 atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa aagataaaaa
    21241 tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga aaagagtaca
    21301 aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc gaacttgttt
    21361 ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgt
//
LOCUS       DAXWLG010000033        21349 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXWLG010000033 DAXWLG010000000
VERSION     DAXWLG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.033"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(58..186)
                     /locus_tag="ACGE4T_000990"
     CDS             complement(58..186)
                     /locus_tag="ACGE4T_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8386286.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="ACGE4T_000991"
     CDS             complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="ACGE4T_000991"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8386287.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1673..2728)
                     /locus_tag="ACGE4T_000992"
     CDS             complement(1673..2728)
                     /locus_tag="ACGE4T_000992"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8386288.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="ACGE4T_000993"
     CDS             complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="ACGE4T_000993"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFF8386289.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="ACGE4T_000994"
     CDS             complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="ACGE4T_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFF8386290.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="ACGE4T_000995"
     CDS             complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="ACGE4T_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFF8386291.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="ACGE4T_000996"
     CDS             complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="ACGE4T_000996"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFF8386292.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="ACGE4T_000997"
     CDS             complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="ACGE4T_000997"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFF8386293.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10490..12049
                     /locus_tag="ACGE4T_000998"
     CDS             10490..12049
                     /locus_tag="ACGE4T_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFF8386294.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12160..14091)
                     /locus_tag="ACGE4T_000999"
     CDS             complement(12160..14091)
                     /locus_tag="ACGE4T_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8386295.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="ACGE4T_001000"
     CDS             complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="ACGE4T_001000"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8386296.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15020..15772)
                     /locus_tag="ACGE4T_001001"
     CDS             complement(15020..15772)
                     /locus_tag="ACGE4T_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8386297.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15931..16140)
                     /locus_tag="ACGE4T_001002"
     CDS             complement(15931..16140)
                     /locus_tag="ACGE4T_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386298.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16151..17479)
                     /locus_tag="ACGE4T_001003"
     CDS             complement(16151..17479)
                     /locus_tag="ACGE4T_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386299.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17615..17770
                     /locus_tag="ACGE4T_001004"
     CDS             17615..17770
                     /locus_tag="ACGE4T_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFF8386300.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17844..18431)
                     /gene="yihA"
                     /locus_tag="ACGE4T_001005"
     CDS             complement(17844..18431)
                     /gene="yihA"
                     /locus_tag="ACGE4T_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFF8386301.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="ACGE4T_001006"
     CDS             complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="ACGE4T_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFF8386302.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20011..21300)
                     /gene="tig"
                     /locus_tag="ACGE4T_001007"
     CDS             complement(20011..21300)
                     /gene="tig"
                     /locus_tag="ACGE4T_001007"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFF8386303.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
BASE COUNT         5632 a         4596 c         3811 g         7310 t
ORIGIN      
        1 aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta
       61 tttattaaag attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat
      121 ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg
      181 gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg
      241 aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca
      301 tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg
      361 attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca
      421 gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca
      481 taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct
      541 ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta
      601 cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg
      661 gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag
      721 attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt
      781 tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc
      841 acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct
      901 ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg
      961 tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag
     1021 atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg
     1081 atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata
     1141 cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt
     1201 ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt
     1261 tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga
     1321 agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact
     1381 tttccatatc caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg
     1441 agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa
     1501 ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc
     1561 atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa
     1621 ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct
     1681 tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac
     1741 gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg
     1801 tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat
     1861 tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc
     1921 gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg
     1981 gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga
     2041 tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg
     2101 cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc
     2161 cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca
     2221 tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa
     2281 cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat
     2341 cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt
     2401 taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga
     2461 tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg
     2521 tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt
     2581 cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt
     2641 cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg
     2701 ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt
     2761 taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac
     2821 cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt
     2881 tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc
     2941 aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga
     3001 cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta
     3061 agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag
     3121 aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag
     3181 cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa
     3241 gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt
     3301 ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt
     3361 aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt
     3421 caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt
     3481 tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg
     3541 ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca
     3601 tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg
     3661 cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga
     3721 tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt
     3781 tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt
     3841 ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat
     3901 atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga
     3961 tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta
     4021 ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac
     4081 caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt
     4141 ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg
     4201 tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc
     4261 aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac
     4321 agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat
     4381 tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc
     4441 gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac
     4501 ttgtcccgct tttttgaagt aagcgtcata ataaccagca cttaaggaga atgtacctaa
     4561 catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc
     4621 atctaatgat ttcacatttt ctgagcgata accgtagcgg ataccatcga atcgttgcaa
     4681 gttggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg
     4741 aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa
     4801 tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat
     4861 cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga
     4921 agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt
     4981 caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa
     5041 acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg
     5101 gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc
     5161 tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt
     5221 tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt
     5281 accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg
     5341 ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt
     5401 atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc
     5461 taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cttcgacctc
     5521 aggctctgtt tctttgattc gttcaaatgt tgcttttgtc aaatcttgta cagtgatttc
     5581 tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat
     5641 gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc
     5701 ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc
     5761 acgtttactg tttcaaccac attcgacgta aatggaacac cttctgtatc tacttcttcc
     5821 agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca
     5881 tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc
     5941 atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg
     6001 ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact
     6061 gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat
     6121 tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt
     6181 ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca
     6241 cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg
     6301 acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt
     6361 aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca
     6421 atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt
     6481 tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt
     6541 cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa
     6601 atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat
     6661 ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc
     6721 ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc
     6781 atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca
     6841 agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt
     6901 cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga
     6961 actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca
     7021 cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc
     7081 cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga
     7141 aacttgtaag ctgctgccca tctcggtgct ttgacagtaa aaccaagttg atcttgtagc
     7201 gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg
     7261 tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc
     7321 cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct
     7381 tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg
     7441 attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata
     7501 tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa
     7561 cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca
     7621 gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc
     7681 acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag
     7741 caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct
     7801 tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa
     7861 ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga
     7921 tattgttcct caattgctac taattctgcg tattctttat cataaacata gtcttccact
     7981 gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct
     8041 tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta
     8101 ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa
     8161 agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc
     8221 ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc
     8281 acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc
     8341 gaatacaggt gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc
     8401 gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga
     8461 gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg
     8521 gcgttcttct tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc
     8581 ttctagacca atcaggaata caactgggaa ttctaatcct ttggctgcat gcaaagtcat
     8641 caatgtcact tgggaagcat cttcttctaa attgtcaata tcggaaacaa gtgccagatc
     8701 atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc
     8761 gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc
     8821 ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac
     8881 ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat
     8941 ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt
     9001 ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat
     9061 agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc
     9121 ttgaggattt gccagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta
     9181 gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat
     9241 tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt
     9301 gcttcgaatc tcttcttgca tccgatctac gataaaacgg gtttcgtctc gttcattatc
     9361 tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt
     9421 tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg
     9481 atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat
     9541 gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca
     9601 caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc
     9661 ttggtactca tctacatgaa tataatggaa tttgttttga taatatgtga gtgaatcagg
     9721 atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt
     9781 atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact
     9841 gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt
     9901 tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg
     9961 tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat
    10021 aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac
    10081 atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt
    10141 attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata
    10201 ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa
    10261 tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc
    10321 agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt
    10381 cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct
    10441 tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa
    10501 aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt
    10561 tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga
    10621 tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc
    10681 tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat
    10741 caagggaagc gggattccac aagtagaggg acagcttcgc ggggaattat caatgaactg
    10801 gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt
    10861 tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca
    10921 attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg
    10981 acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca
    11041 tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt
    11101 cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt
    11161 gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt
    11221 tatttatcag aaggtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc
    11281 ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag
    11341 tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat
    11401 tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggtgg
    11461 tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta
    11521 tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat
    11581 tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt
    11641 gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc
    11701 ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct
    11761 acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt
    11821 aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat
    11881 gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat
    11941 gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca
    12001 ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa
    12061 aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa
    12121 agaagagact gggaaaaacc agcctcttct tttttatttc tatgcttctg ctgctgcagg
    12181 agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag caataaacac
    12241 caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccat gaggagccag
    12301 taatttgatc ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact
    12361 tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga
    12421 tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt
    12481 aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga
    12541 agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac
    12601 ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc
    12661 accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt
    12721 gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa
    12781 cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa
    12841 tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa
    12901 tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg
    12961 gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc
    13021 ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg
    13081 atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc
    13141 accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg
    13201 tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa
    13261 tactggtgca gtaccactaa gggctagatt gatcagctct tctggtttac ggataccatc
    13321 actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc
    13381 tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat
    13441 tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt
    13501 cagagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac
    13561 aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt
    13621 ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt
    13681 atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc
    13741 taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata
    13801 agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac
    13861 tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc
    13921 tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat
    13981 ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc
    14041 ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct
    14101 ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg
    14161 cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag
    14221 gcttcgattg gatctttcgt acggctgtaa gccccaataa agccggcaat cattgaatcc
    14281 cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt
    14341 tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca
    14401 gctagcaatt ttttaccata cggaatgatt tcttctacag attggaaggt tgtattgtaa
    14461 agctcagcta attcgtgatt gtttggtttg atcaacaatg gtttcttaac aagtgcattt
    14521 tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca
    14581 atcagttctt gataaaatcc ttttcgcagg ctagccggcg cgcttcctga caatacgacg
    14641 atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa
    14701 atagatgggc ccaaaccgtt aatttcagtt tcagtatctg attttagttt gatattgatt
    14761 cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc
    14821 gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc
    14881 cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc
    14941 attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga
    15001 ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt
    15061 cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa
    15121 cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg
    15181 aatttttcat ggctaaacgt ttaacagctg cctcttctgg gtccggagtc gtgaatcctt
    15241 gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt
    15301 gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa
    15361 taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta
    15421 ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta
    15481 aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt
    15541 cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt
    15601 tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac
    15661 gtcttatcgt tgattcagaa gcattaaaaa ggtttacaag atcttgtgat ttcacgattt
    15721 tctgctgttc aagcaagtct aggatttttt gtctacgttc ctctgtaagc attttcacga
    15781 tcacctcaat acaaataata gcatattttt tttctactcg caatataaat cagtcaaaaa
    15841 cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaaa aagactggtt
    15901 ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt
    15961 tttcatataa acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat
    16021 cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa
    16081 gagacagacg gataataatg atgccggaac cattattcga taatagagtt gtttatccat
    16141 tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa
    16201 attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt
    16261 tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt
    16321 ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt
    16381 catctgttac ctctacttct agatattccc taatataata acaataagct tcgattccat
    16441 tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgcgag cgattctcgt
    16501 caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat
    16561 ctccttgttg aatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag
    16621 gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt
    16681 caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa
    16741 gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt
    16801 ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt
    16861 ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt
    16921 cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc
    16981 aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct
    17041 ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat
    17101 aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa
    17161 tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc aataattgat
    17221 tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt
    17281 ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt
    17341 gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat
    17401 cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata
    17461 atttatcttc gccaatcatt ttctcacctc tttaaaatct ttttataatt taattataac
    17521 caacgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat
    17581 agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga
    17641 taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa
    17701 agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa
    17761 acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga aagagtttga
    17821 ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc tgcccacgct
    17881 tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa
    17941 tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg gatcttatct
    18001 gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg atacatctgg
    18061 atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc acgtagctgg
    18121 tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc agttttggat
    18181 acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc gatcaaataa
    18241 aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt ctttcgatca
    18301 atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag agctatttct
    18361 ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat ttcagcttga
    18421 tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa
    18481 agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct ctgaatcttt
    18541 atagattact gttggctcac cagacaattc agctgcttct tttgtgataa tcactttttc
    18601 gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt caatgatgga
    18661 acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc
    18721 ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat attgtttgac
    18781 tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca atttctctaa
    18841 agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt ttagtaaatc
    18901 ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt tttgtgtacc
    18961 aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc catcaaatgc
    19021 accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt gatgtggatg
    19081 tttacgtcct ccctgaggtg gcacgcttgc aacagttcct tctagaattt tcagcaaggc
    19141 ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc ttttacgcgc
    19201 aattttatcg atctcatcaa tatagatgat ccctttttct gcacgttcca cattgtaatc
    19261 agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat aaccagcttc
    19321 tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg ctaatgtttg
    19381 tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc ttttttgcag
    19441 ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt gattgtatac
    19501 ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc
    19561 aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt cataaaattc
    19621 ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga cccctggacc
    19681 agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc gtacggtctc
    19741 attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa gtatgcaaga
    19801 aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa aagaaaaagt
    19861 gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac aaaaaataag
    19921 gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt ctagaagtag
    19981 cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg ctgtacctgt
    20041 gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt ctgtcaacac
    20101 gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga tttctgcatt
    20161 gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga tcacaagatt
    20221 tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt cttctgtaga
    20281 accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt tgttcaagaa
    20341 ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt caacgatttc
    20401 tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc
    20461 ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg ttgatacttc
    20521 gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg cttttacttc
    20581 atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt gataatcttc
    20641 tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta attgatcttc
    20701 aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc cttttccgcc
    20761 atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat cgccattttc
    20821 agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac gtttgatacg
    20881 tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta gatctttata
    20941 ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc agtcttgacc
    21001 tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt
    21061 gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat ataaagcttc
    21121 ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga atccaggtac
    21181 gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga tttcgtcttg
    21241 cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat
    21301 ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttc
//
LOCUS       DAXWLG010000034        20261 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXWLG010000034 DAXWLG010000000
VERSION     DAXWLG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.034"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(60..638)
                     /locus_tag="ACGE4T_001008"
     CDS             complement(60..638)
                     /locus_tag="ACGE4T_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8386304.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(715..1245)
                     /locus_tag="ACGE4T_001009"
     CDS             complement(715..1245)
                     /locus_tag="ACGE4T_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFF8386305.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1249..2109)
                     /gene="rlmB"
                     /locus_tag="ACGE4T_001010"
     CDS             complement(1249..2109)
                     /gene="rlmB"
                     /locus_tag="ACGE4T_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFF8386306.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2102..2497)
                     /locus_tag="ACGE4T_001011"
     CDS             complement(2102..2497)
                     /locus_tag="ACGE4T_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFF8386307.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2494..3906)
                     /gene="cysS"
                     /locus_tag="ACGE4T_001012"
     CDS             complement(2494..3906)
                     /gene="cysS"
                     /locus_tag="ACGE4T_001012"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFF8386308.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3903..4439)
                     /gene="epsC"
                     /locus_tag="ACGE4T_001013"
     CDS             complement(3903..4439)
                     /gene="epsC"
                     /locus_tag="ACGE4T_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFF8386309.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4525..4764)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4761..6221)
                     /gene="gltX"
                     /locus_tag="ACGE4T_001014"
     CDS             complement(4761..6221)
                     /gene="gltX"
                     /locus_tag="ACGE4T_001014"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFF8386310.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6391..7548)
                     /locus_tag="ACGE4T_001015"
     CDS             complement(6391..7548)
                     /locus_tag="ACGE4T_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFF8386311.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7582..8952)
                     /gene="radA"
                     /locus_tag="ACGE4T_001016"
     CDS             complement(7582..8952)
                     /gene="radA"
                     /locus_tag="ACGE4T_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFF8386312.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9015..9542)
                     /locus_tag="ACGE4T_001017"
     CDS             complement(9015..9542)
                     /locus_tag="ACGE4T_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFF8386313.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9772..10578
                     /gene="proB"
                     /locus_tag="ACGE4T_001018"
     CDS             9772..10578
                     /gene="proB"
                     /locus_tag="ACGE4T_001018"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFF8386314.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10575..11822
                     /locus_tag="ACGE4T_001019"
     CDS             10575..11822
                     /locus_tag="ACGE4T_001019"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFF8386315.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11898..12242
                     /locus_tag="ACGE4T_001020"
     CDS             11898..12242
                     /locus_tag="ACGE4T_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386316.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12359..12685)
                     /locus_tag="ACGE4T_001021"
     CDS             complement(12359..12685)
                     /locus_tag="ACGE4T_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386317.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13058..14259)
                     /locus_tag="ACGE4T_001022"
                     /pseudo
     CDS             complement(13058..14259)
                     /locus_tag="ACGE4T_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14376..14891
                     /locus_tag="ACGE4T_001023"
     CDS             14376..14891
                     /locus_tag="ACGE4T_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8386318.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15090..15536)
                     /locus_tag="ACGE4T_001024"
     CDS             complement(15090..15536)
                     /locus_tag="ACGE4T_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFF8386319.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15583..17061)
                     /gene="msr(C)"
                     /locus_tag="ACGE4T_001025"
     CDS             complement(15583..17061)
                     /gene="msr(C)"
                     /locus_tag="ACGE4T_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8386320.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17415..18044)
                     /locus_tag="ACGE4T_001026"
     CDS             complement(17415..18044)
                     /locus_tag="ACGE4T_001026"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8386321.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(18028..18492)
                     /locus_tag="ACGE4T_001027"
     CDS             complement(18028..18492)
                     /locus_tag="ACGE4T_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8386322.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(18979..20247)
                     /locus_tag="ACGE4T_001028"
     CDS             complement(18979..20247)
                     /locus_tag="ACGE4T_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8386323.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT         5692 a         4346 c         3667 g         6556 t
ORIGIN      
        1 cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat ttttctaaat
       61 tattcctcat tataaaattt caagtgtttt tttattttga gttttactct cccgtaagcg
      121 ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa atcaacgcct
      181 tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc gcaattttcg
      241 attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt gatctctgct
      301 aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc tttttgatca
      361 cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct tataatgctt
      421 ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa tgcctgatat
      481 aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa ataataggtg
      541 tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa tgcttcttcg
      601 tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct ccataaattc
      661 gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga tatgttattt
      721 attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct cttcattcca
      781 tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg ctattgtttt
      841 ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg ctccttggga
      901 aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg tcaaacggtc
      961 attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt ctttcgtaaa
     1021 aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta gttgggcatc
     1081 gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac gatgtaaaag
     1141 agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt ccggccacga
     1201 accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct actccccctg
     1261 acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat ttaagctctg
     1321 gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca atcctggact
     1381 cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat tccatttttg
     1441 gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt tgagctgttg
     1501 cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg ccccagtgga
     1561 agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga tcacaccatc
     1621 gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat cttcgatgct
     1681 atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa tcagttggtc
     1741 aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca cgccgtgatc
     1801 gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt gttcacgtgc
     1861 gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa agagtttatt
     1921 ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga aattatcttc
     1981 taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct ttttatttga
     2041 atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt ttgttctttc
     2101 tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct tccatccgct
     2161 cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt gcgacccgat
     2221 acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt cctctgcgat
     2281 aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga atcaaaaagg
     2341 cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg ggttttgttt
     2401 ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct ccaacataag
     2461 ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc atcgggttcc
     2521 ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa tttcgtcgct
     2581 tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt gttcaatctc
     2641 ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc attgcgagaa
     2701 taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta gataagtgtt
     2761 cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa aatcatcgtc
     2821 catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat ccgcatcatc
     2881 tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg tattcttcag
     2941 tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga tcggacgacg
     3001 gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt tgatcatttc
     3061 atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt cgccaatcgt
     3121 tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg cttcactttg
     3181 tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat ggatatcgat
     3241 cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac cgggacgtcc
     3301 tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc ataaagcgaa
     3361 atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg ctccgacttc
     3421 cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac gggtacggta
     3481 atacacatca cctgaagatt cataagcata gcctttttca accaacactt gaataaatgc
     3541 taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct cgacattcaa
     3601 cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg gtgctgtaat
     3661 acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat tcgatacgaa
     3721 atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag caatggtgct
     3781 acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat acataccgac
     3841 atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat tgtagatttt
     3901 tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc tggtactcct
     3961 accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc gattttggcg
     4021 ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat ggctccttgt
     4081 ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt tacgccatgg
     4141 aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc cattccatgg
     4201 tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc agttaggaaa
     4261 cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata taaaaaatga
     4321 gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat ctcagctgtc
     4381 gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa ccaactcatg
     4441 actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata aagaaaagaa
     4501 gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc acttttcttc
     4561 ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct actttgtttc
     4621 ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag aggcatttcg
     4681 gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat cagctgctta
     4741 cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa tgatttttca
     4801 cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc tgatacagct
     4861 acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg aacagctttg
     4921 atggcagctt tgacagttgg agcatctact acttccattt gttctagctg tgctttgaat
     4981 gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc ttcattcaat
     5041 acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc atagctcatt
     5101 tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc atctgggttt
     5161 tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa tgcatctaga
     5221 tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc gaaagctgcg
     5281 ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg ttcgaagatt
     5341 tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat tgcatctggt
     5401 agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc acgtttgctt
     5461 agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt tggtggtgtc
     5521 cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg gtcatctcca
     5581 cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa attataagtt
     5641 ggcataccat cgcgtttttg tatgacaaag tcgccaccga tattatcgga ttcgaacgaa
     5701 atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac acggaaacgt
     5761 acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt caagttcgca
     5821 catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg ttctgcttct
     5881 aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag ctgatcaatc
     5941 agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc tccaggattt
     6001 tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg gcttttttcg
     6061 ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa gtctccatca
     6121 ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat atgcaggtgc
     6181 ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct cttctttcaa
     6241 actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat atcatttatt
     6301 taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc aactggttga
     6361 atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct ttttctctgt
     6421 ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga tcatgcgtcc
     6481 agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca tgtaatgctg
     6541 tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt gttggcgttc
     6601 tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga ctggttttac
     6661 ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag agactttatt
     6721 caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt tgatcaattt
     6781 gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag atataccctc
     6841 ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac gtttcaaact
     6901 atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga tcatcaaggt
     6961 tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga tcacgcttgt
     7021 atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca gttgactctc
     7081 aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa ctttcttcca
     7141 ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga tcatcagtaa
     7201 aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg tcgagatcac
     7261 aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat aagtcaagct
     7321 gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt tggccaacag
     7381 caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg tattattcag
     7441 ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg aaatacccaa
     7501 gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg tttttcctcc
     7561 gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag ccaaagttgc
     7621 tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt ttttaggtag
     7681 gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa tatgactgac
     7741 acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg gctgtgttcc
     7801 tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg ctggttcatt
     7861 taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa gcaacccagc
     7921 tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac ctgttgttgt
     7981 tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta ccaaaattgg
     8041 acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta ggcgttcttc
     8101 taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca tttcaaaaat
     8161 cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc gaaaactatg
     8221 atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca ttcttgggcc
     8281 ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc catttgtttt
     8341 agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc ctgccacact
     8401 tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat caggggagat
     8461 atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat aaaattctgt
     8521 gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt cttcgccgga
     8581 tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta acagcaaagt
     8641 ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca ccacacctcc
     8701 gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt ttttagggac
     8761 gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc gagttctgcg
     8821 gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc aatttgggca
     8881 gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt gtacttttga
     8941 tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa tcaagcttaa
     9001 gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg agcagtcgtt
     9061 tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg ctcgcctttt
     9121 ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg gccttcattt
     9181 gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa cactaaaaac
     9241 cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc tcccatgtaa
     9301 gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc tgatacatct
     9361 tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt ccagatactt
     9421 ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg tgtagcgcgc
     9481 tgcggtagat tgatgttttg atctttatat ttagaaataa cttcaaatcc tctttttctc
     9541 accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc tgtaaaagac
     9601 tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta attcgttctt
     9661 tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt actcgtcaaa
     9721 acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa
     9781 gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt gatttatccc
     9841 aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc tgatcttaga
     9901 aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg tatgcataaa
     9961 ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc ggctgttggt
    10021 caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca acagacggca
    10081 cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat
    10141 acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa cgatactgta
    10201 gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct gtcagccatc
    10261 atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga cgggttttat
    10321 tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga taaaatcgat
    10381 gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg cggtatgcag
    10441 agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat tttagcaaat
    10501 ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg tactttattt
    10561 aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa gatagcgcgc
    10621 aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa atggcacaaa
    10681 ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta gaaaaagcag
    10741 tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct gagcgaatca
    10801 atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag
    10861 atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg ccactaggcg
    10921 tgaccggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct agtctagcat
    10981 tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc tcgaatcaat
    11041 tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct tatgccatcc
    11101 agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg acagaatact
    11161 tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa
    11221 cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat aaagaagcac
    11281 agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta
    11341 acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg ccagctattg
    11401 aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc
    11461 tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta gattatatcc
    11521 ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat cggcataata
    11581 ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt ttcttaaatg
    11641 aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac ggatcggtat
    11701 ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg
    11761 ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa atccgcgaat
    11821 aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag gttctatcca
    11881 actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac cttatttctc
    11941 tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa ttcaaaaaac
    12001 gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag
    12061 ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta gcgacaatca
    12121 attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg attgctggga
    12181 tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg actttccgct
    12241 aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt
    12301 ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg gcgttcgcct
    12361 accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt
    12421 cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt tctttttctt
    12481 cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca gctatttttt
    12541 gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct ttgatatagc
    12601 cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga
    12661 cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt aaatgaattt
    12721 tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg gaatgtgaca
    12781 aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc
    12841 ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc cttgtgattc
    12901 agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaaaagct tgcatctgcg
    12961 gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga
    13021 cttgatgcag gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa
    13081 aataattacg gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat
    13141 aaaacgcata gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag
    13201 ctccaccgaa aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg
    13261 gtatttccat agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta
    13321 acacaatgct gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac
    13381 aaataatgaa taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa
    13441 gagccggtgc aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa
    13501 aaccgacttc taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa
    13561 agaatccgaa attatataac aatgcgatta gtccaactga acgaagttta tgattgctta
    13621 acgctttgac accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa
    13681 gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac
    13741 gccatgaacc actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa
    13801 gtgctgcttc atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg
    13861 ttgctaaagc agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa
    13921 tcagcattcc gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca
    13981 atcctgtcat caatgttttt tcaaaccgat ccgactagat aagaaacctg taaatagcat
    14041 caccacacca gtgactaaca tgtaactagt aaataacagg gtcgtttcag ctggcgtagc
    14101 gtttaactgt gcagcaatcg tttttaagat tgggtctact agacccactc ccataaaagc
    14161 gatcatacag gtagctgcta caactagcgc tgaacttttc ccctttgttt cttcatttgt
    14221 ttgttcttgt atatctattt gattctccaa tatttccatc cctttctctc tattagcatc
    14281 atacatgatt tattttacat gtaaatatgc acatgtaaaa cgtggaaact ttactgtgat
    14341 ataataaaga aaataagacg agaggtagga tttttatgaa tactttagcc tatatattat
    14401 tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt gaagcagctg atcacaattt
    14461 tttgggaagc ccatcacagt cagatctata caacgcttgc caagttagac cagcagggtt
    14521 atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa gatctaccac ttgactgatg
    14581 aaggacgtgc tcttgtagag gaatggatca aagaggaaac tcccgctcct acgcaaaaag
    14641 atgaatttct agcaaaaatc tatgcttttt cgacgctcga taaaacaaca gctactggac
    14701 tgctattcac tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga
    14761 atgacctctc gctagacaag aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa
    14821 ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa atgcagcagt
    14881 tttttgagta agggataaac agaacctttc tagaaatatc tattgaagag caaattatct
    14941 tcaataaaag agcaatacag ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct
    15001 atcgttcgga tcactatttt tctagaactg cattcaagag actatgacaa aaagcatgcc
    15061 aaggcctttt ttcaaataac agtgttccat cattcattac tttgaattgc ttttagcaca
    15121 cttcttgaaa gcgggccaac gatgatcaac tgcaacggta aagcggcaat cacattcatt
    15181 ttccaagcat tcatataagc agagaataat tgatcaggta ttccttgttc catgatcaca
    15241 ccgaataaag acatacatgt caccattcct aaaaccatca agcatgaaat agttaagatc
    15301 atttggacct tgctttctgg atttactggt agtttaaagg caattttttt cgccagcaca
    15361 ccgatgatga agacatccaa aatgaaagcc accacgaatc caggcactag accaatcaac
    15421 aaacttgata aatgaagatc ttgatgcagc cataaattat acgcgctcat ccccaagacc
    15481 atcaaaaaac acatcattgt tgtaaaaatt attccttctt ttttatttgt aggcaatctg
    15541 taccactcct ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag
    15601 tacttttttt attttctgcc cttttatttc ataaacttca tcagccgtac gctcgacaaa
    15661 gtaagaatca tgagaagtaa acaaaatcgt tcccggatat atttgcatta gcttctctaa
    15721 agcttcgatc gttgccatat caataaaatt agtcggttca tctaacagca acacattact
    15781 tggcttagta aaaagcaaag caatcgtcag acgggtcgct tctcccccac ttaatgtaca
    15841 taacggtttc gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac tacggacaag
    15901 cgattctgaa aactctgttt gtctcatgac aaaacgaatg atggattctt cagatgtcat
    15961 ttgataaccc ttttgctgat atacttgaaa gcttactttc ggagagagaa ggattccttc
    16021 tccttgtttg cggatatgtt ctaataaggt tgtcttacct gaaccatttt caccgacaat
    16081 cgcgattttt ttacctaaag aaaactgaaa atcaccattt accagtaatg ttctcccact
    16141 tctttccaat tggacatttt gtcccatgat gggatagcgg ctgtggagtt caagagattt
    16201 tggtactgga aaacggattt gtttcatctg ctgcggcttg gttgtttctc ctattttttg
    16261 gagtcttctc tctaatgttt tcgcattctt ttggatggct ttttgtacac tgtctttttg
    16321 tttagaggaa gacaaccgat ccggtctgat ttgttgcttt ttctttttac tggacacttt
    16381 ttccaacttt tccgcttgtt ttttcttttc ttggatcgat ttttccaatc gttttttctc
    16441 tttcatgatc gactctgctt ctcgtagctg agtctttttc tccaattcct tttgagtgag
    16501 ataggcacta taattccccg aaaactctcg gatttctccg tcttttactt cccagatttt
    16561 cgatgctagc tcatctagaa aatagcgatc atgactaaca aaaaccagcg taccataata
    16621 ataacgaagc tcttcaatca gtcgatcaat actttttcta tctagatggg ttgttggctc
    16681 gtctagaagc atcccttgtt cataagaaga aagaagacgt atcaactcga tctttcgttc
    16741 ttcccctccg ctccatttcc gctctgaagt ttcttccagt cttaactgga aatgactggc
    16801 taattccaaa tttaggtcct ttgcttcagc gagataggtt aattgaggca ggtagttgaa
    16861 ggtgatctct gtttggattt tcccatgatc gggaaaaagt tcaccggcta ttagtttgag
    16921 caaagtcgat ttaccgacac cattttctcc tatgatccct atacgatctt gctgatagac
    16981 acgaagagaa tcaatagata gttctagctg gttgccaaag ctgacttgta gatttgttat
    17041 atttactgct aaattttcca tatgttttac actcctattt catgtaaaac aacaattatc
    17101 gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat cttttcctac
    17161 tctgaacgaa ataactatga ctaaatcacc aaatctgtca tagctttcac ctattctact
    17221 gtcgttcgaa aaaatggaca tactccatcc atgaaattgt tgtttataca tgtcttttga
    17281 atttatttag ttgttattca aaagttcgaa acgtaatttc atcgatgcag tcatttttcc
    17341 gacctccttt tttattaggt taccacacac gattcaaaaa aagcaatagc tactgaatag
    17401 taactgaaaa ccttttattc gaacgtagtg atcttttctg gtgctaaacg aacagactcg
    17461 taaccttctt ctaccgcttt tcctattgat aaaataataa ctggaacata acggtcttta
    17521 tccaaaccaa atgcctctgc caactgatct gcttcgaatc caccaattgg atttgtttca
    17581 tagccatgat cacgcgcaac taacatgaat tgcatagccg ctaagctaga atcgactttg
    17641 actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat
    17701 tgttgatctc tgacttcttt tggcatcttc cctgattcgt aagcttggtt atagatttct
    17761 tcgccatatt cgtaacattg catatcgcca aaaatcatga ccattgcaga agaagtatca
    17821 ttttgtctcg tattgaaacg gatcaaagga cgcaagattt cttttgcttc ctcgctttca
    17881 gcgaccacaa agcgccatgg ttgcatattg acagaagaag gtgcagtcgt cgcttctttg
    17941 atcatttcca gcatttcttc gtgtgggatt ttgtatgttt catcatatac gcgaacggat
    18001 cttctaccag tcataattgt tgaaaaatca tttttttgtt ccattattat taacctcttt
    18061 cgtttctgtt gttatattgc aaagttgggt attcaattta tctaaaagtg ttagaaattg
    18121 tttcatttct acatcgctga aagccgggga tagcacggaa cctgtatctt tatggtccat
    18181 ttcacaatgc tcgattttct tctgggcttt ttctgttagc gctacataca cttcccggtt
    18241 gttctgagga tttctctgac gcgaaacata gccatttgct tccaatacct tcagatgacg
    18301 agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt
    18361 ttcttctttc agtctcatca tgatctcgta tctagtaaga cttataccga tattttcttc
    18421 aaagatctgg acgatttttt gatcggctaa tttgatttga tataaaaggt gactaatctc
    18481 ttctaaagac ataatttcct cctttctaat aattgactcg tcaacgattg acttgtcaat
    18541 aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa aacgctctca
    18601 atagaaaagt atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa
    18661 gcacaacgct aaaaccaaga ttattgattc ttatcaaact atagaagata gctcttctgc
    18721 agtagaaaat tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga
    18781 atattagaaa ttggatcagt atgcttaaac agtaataaaa aagttatgac aagatgttgt
    18841 cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc
    18901 aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct tactacttga tgcagggcag
    18961 ctttttcact acctcttttt aataaccata aaatcctatt ccttcatatc tccagcccat
    19021 cggtgttagc caatctcttt ccttagcact caaggtatag tgatgggtac cagttttttc
    19081 atttggatta aatagtcgat aaattggctt ggaaccacca gaataaaaac ttagaccttc
    19141 ataacgccaa ccgatttttg ttaaagagtt gatctcagat tgtgccattg tgtaatgatg
    19201 gtctccactg taaggattgt ataaacggta gacaggttgt ccgctgtttg gtgcttgcca
    19261 agcaatccct tcatattccc agcctacagt tggcagataa tttttttctc cggcattcaa
    19321 tgtatataaa tgttctcctg tatgacggtt atacagacga tacatcggag aagttgaagt
    19381 tggtgctgta tcatactgtg tcagattata ggtttcgatt acattgttca atgtagaacc
    19441 ataattaggc gcagtggcat aacgacctgt tagccaagcc gtagcatctt tgtaagagtt
    19501 cgtattgctt ttccatgcac cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt
    19561 caataccgtt acgttatctt gcaatgattc tttgtacgaa ggatattttc taaaagcggc
    19621 attcaccgta atccattgcc cattcacata ttccgaagta ggcatcgtaa cagattggcc
    19681 attatagctt cctttaatcc caaatagatt gtaattaggg gcagaagcca aagtactgtt
    19741 cccccagcca ctttcaacga tggcttgagc aatcatgact gatgcatata agtcattggc
    19801 acttgccaaa ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt gagaattagc
    19861 agatgagcga gcataaaaag catagccagt atagttttcg aattcagaca aatctgcttg
    19921 atgggtatgt gtcaatgttt gcccatctgt caattggttc gtgatttctt ctgcattctc
    19981 tggattgttc tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc
    20041 agttgttggt tcttcggttg aaattgtatt ttctgagaca gtagctgact gcaaagtact
    20101 ctctgtatcg ttttcaattc ctgtttctga agaactcgat gtctgagttg cgacagtttc
    20161 tgtcgattct tcagctagca ctgttgtgtt ggcaactgct agatttccta aaagaataac
    20221 gctaaataat aaattctttg ctttcatctt ttttctcctt t
//
LOCUS       DAXWLG010000035        20019 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXWLG010000035 DAXWLG010000000
VERSION     DAXWLG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.035"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..117)
                     /locus_tag="ACGE4T_001029"
     CDS             complement(<1..117)
                     /locus_tag="ACGE4T_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFF8386324.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            296..1957
                     /locus_tag="ACGE4T_001030"
     CDS             296..1957
                     /locus_tag="ACGE4T_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8386325.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2027..2374)
                     /locus_tag="ACGE4T_001031"
     CDS             complement(2027..2374)
                     /locus_tag="ACGE4T_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFF8386326.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2458..3399
                     /gene="opp3b"
                     /locus_tag="ACGE4T_001032"
     CDS             2458..3399
                     /gene="opp3b"
                     /locus_tag="ACGE4T_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8386327.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3400..4443
                     /locus_tag="ACGE4T_001033"
     CDS             3400..4443
                     /locus_tag="ACGE4T_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386328.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4449..5519
                     /locus_tag="ACGE4T_001034"
     CDS             4449..5519
                     /locus_tag="ACGE4T_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386329.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5509..6462
                     /locus_tag="ACGE4T_001035"
     CDS             5509..6462
                     /locus_tag="ACGE4T_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386330.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6474..7484
                     /locus_tag="ACGE4T_001036"
     CDS             6474..7484
                     /locus_tag="ACGE4T_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8386331.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7769..8590)
                     /locus_tag="ACGE4T_001037"
     CDS             complement(7769..8590)
                     /locus_tag="ACGE4T_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8386332.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8687..8965)
                     /locus_tag="ACGE4T_001038"
     CDS             complement(8687..8965)
                     /locus_tag="ACGE4T_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386333.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9233..11242
                     /gene="metG"
                     /locus_tag="ACGE4T_001039"
     CDS             9233..11242
                     /gene="metG"
                     /locus_tag="ACGE4T_001039"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFF8386334.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11721..12308)
                     /locus_tag="ACGE4T_001040"
     CDS             complement(11721..12308)
                     /locus_tag="ACGE4T_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386335.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12483..13805
                     /locus_tag="ACGE4T_001041"
     CDS             12483..13805
                     /locus_tag="ACGE4T_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8386336.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13960..14736
                     /locus_tag="ACGE4T_001042"
     CDS             13960..14736
                     /locus_tag="ACGE4T_001042"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8386337.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14729..15298
                     /gene="rnmV"
                     /locus_tag="ACGE4T_001043"
     CDS             14729..15298
                     /gene="rnmV"
                     /locus_tag="ACGE4T_001043"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFF8386338.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15291..16175
                     /gene="rsmA"
                     /locus_tag="ACGE4T_001044"
     CDS             15291..16175
                     /gene="rsmA"
                     /locus_tag="ACGE4T_001044"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFF8386339.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16626..17255
                     /locus_tag="ACGE4T_001045"
     CDS             16626..17255
                     /locus_tag="ACGE4T_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386340.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17379..18488)
                     /locus_tag="ACGE4T_001046"
     CDS             complement(17379..18488)
                     /locus_tag="ACGE4T_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386341.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18697..19119
                     /gene="mgsA"
                     /locus_tag="ACGE4T_001047"
     CDS             18697..19119
                     /gene="mgsA"
                     /locus_tag="ACGE4T_001047"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFF8386342.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19306..19404
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19442..19978
                     /locus_tag="ACGE4T_001048"
     CDS             19442..19978
                     /locus_tag="ACGE4T_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8386343.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6515 a         3599 c         4083 g         5822 t
ORIGIN      
        1 gataagggac tgtgacaaga cttttgtcac agtttctttt tgtttattct tttttatctt
       61 tgtatggcaa taatagacaa tataacgaaa attatttaat ggcttttgga agaccatgga
      121 aaaccttgta caaagctatt ttttctgaaa aattccttga aacctcacga aaaaatcatt
      181 attttatgaa aaagacaaaa aaaagtgttg attttttaat aattattcat taaactctta
      241 aacaacttca gaaaatatta attattctga aaattccaat aaaggggtac gtaaaatgaa
      301 aaagaaattt acatttggtt tggttgcggt atgcggcctt gtacttgctg gatgttatgg
      361 cggcagttca gccacaagca gtagttcagc aaaaagcggt tcagcagatg gaggaggcgt
      421 atttaactta gtcgtgcctc aagaaatgcc tacagctgac ttatcagttg caacagatac
      481 aatcagtttt acagcattga ataacgttta tgaaggaatt taccgtcttg atgaagacag
      541 caaaccacag ccggcaggag ctagcgagtt ggcagaagtc agtgaagacg gattaactta
      601 caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt gatcctgtta cagcagcgga
      661 ttacgtttat ggctggcaac gaacagtaga cccagctaca gcttctgaat atgcttacct
      721 gtatgcgcca gttgaaaatg cagaagacat tacagctgga aagaaagata aatcagaact
      781 agggatcaaa gcagttggtg actatgaatt agaaatcaaa ttgacgaaac aaacaccata
      841 tttccaatat ctgcttgctt tcccatcatt tttcccacaa aaacaatccg tagtggaaga
      901 aaatggggat gcctatgcat ctgcaagtga taaagctgtt tataatggac cattcacatt
      961 agaagatttt gatggaccag gaacagatac agaatggact tataagaaaa atgatcatta
     1021 ctgggacaaa gatacagtaa aactttctga aatcaaagtc agtgtagtaa aagaatcttc
     1081 tactgcattg aacttattca aagatggcca agcagatgac gtgatcttgt ctggtgagtt
     1141 agcacagcaa aacgcaaacg atccagctta tacttctgtt aaagaagcgc ggacaagcta
     1201 tatcgaattc aatcaacgtg aaaagaattc accattcaac aatgtgaacc taagaaaagc
     1261 tatttcttat tctatcgatc gtgaagcttt agtaaaacaa gtactaggtg acggatcagt
     1321 tgtctctaca ggattgatcc catcagatat ggtaaaaagt ccaacaacga acgaagactt
     1381 tgcaaaagaa gctggaaaat tagtttctta tgacaaagat aaagcaaaag aatattggga
     1441 aaaagcgaaa aaagaattgg gcgttgattc tttagaattt gatttgatgg cttctgatga
     1501 tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca atccaagaaa acttggatgg
     1561 cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt cttgaccgct caacttcggg
     1621 tgattttgac gcagtactag gcggatgggc tgctgactat gcagacccaa gcagctttac
     1681 tgatttgttt gtcacaggta attcatacaa ccgtggtcgc tggagcaatg cagaatatga
     1741 cgaagcagtt aaagcagctt catcaacaga cgctggcgat gaacaagccc gttgggatga
     1801 tttccaaaaa gcaatcaaga tcatctctga tgatatgggt gtcgttccgg tgtaccaaaa
     1861 agcagaaggt cacctagtga atgaaaaaat caaaggtatc gttcaccatg cggcaggcgc
     1921 ttcatgggat tacaaatgga catacgttga agaataatcg ttaataccgt gagcatgaga
     1981 aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat attcacttat cctaacaaag
     2041 aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc agccaaaagc
     2101 tgtacatacc gtagctggca gacgagagcg aggaaaattc tggttttttc tttcggttcc
     2161 ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa gaaaagaccc
     2221 ataaaaaacc gccgataatc agaaacggca atgaccataa gaatagatca ttagataatt
     2281 gaatgagtgt aaaggaatgg ttgatgagat tttttattaa aataaccaga atcagtagaa
     2341 aagctactgc gtaggggata gatttttttt tcatttgatt gctcctttat ttttggttca
     2401 ttatctaata atgtagcgaa acatctgtca gaagtcaaaa gctaggggga aacattagtg
     2461 aatagttata tcaaatatgt attaaaacgt gtctttttca tgatcatcac gctttggtta
     2521 attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg gcacgccata tacgaatcag
     2581 gaacgtttga gtcctgaaac aatcgagatg ctaaataaac aagtcggttt ggataaacca
     2641 gttatcgtac aatatgggat ttatctttct aatttgcttc aaggagattt tggaatttcc
     2701 ttccaattta aaaaccagcc agtcgcacat ttgttagctg gaagaatcgg accatccttg
     2761 cagttagggt tacaggcaat tatctttgga acagtcctag ggacgatttt aggaacgatc
     2821 tctgccatga agcaaaatac atgggcagat acatcttcta cactcgtagc gatattaggt
     2881 cgttccattc caaactttgt atttgctgtt ttattacaat atatctttgc aatcaaatta
     2941 caagttttgc caatcgcaaa atgggatggc tttgtgtaca caatcttacc aacaattgcg
     3001 ttggccatgt cgccgcttgc tgactctgct cggtttatcc gtacggaaat ggtggaagta
     3061 ctgcatagtg attatgtgga attagcaaaa gcaaaaggat tgagccgttg ggaaatcgcc
     3121 tttaaacatg gtcttcgaaa tagtttgatt ccattgatga ctttattagg accgttagct
     3181 gtagcattga tgaccggatc actggtggta gaaaatatct ttgctattcc tggtatcggg
     3241 gaacaattcg ttaagtcaat cacaacaaat gattatccta cgatcatggc tgtaacgatc
     3301 ctttactcat ttatgctgat ttttgttatt ttgatcgtgg atctattgta cggtctagtt
     3361 gatccaagaa ttcgtgtttc agaagggagc cgaggataaa tggaaatgat accaaagcgt
     3421 tatgaaacag ttgcagatat ccctgcggat gaatttcttc cattacaaaa aaataccgaa
     3481 gaagaacgtg aacagattac tgcaccttct ttgaatttta tccaagattc atggcgccgt
     3541 ttaaagaaaa acaaagctgc cgttatttca atggttttac tattggtcat cattttgatc
     3601 tctgtgatca caatctttgt ttctcctcat gatccaactg cccaaaacgt ggcttatatc
     3661 aatttgcctc ctcgtattcc gggaatcaat atcgatggat tgaatggaaa agcaatggtt
     3721 ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt aaatttcttt
     3781 ttaggaacag acggtttggg acgtgatgta ttaagccgct tatttatggg aatgagaatc
     3841 tcgttgctga tcgcatttat tgctgcatta ctagatgtaa cgattggtgt agcttacggg
     3901 ttggtttctg gtttgttagg cggacgtgtc gataacgtga tgcaacgttt tttggaaatc
     3961 ttatcaggaa ttccaaacct cgttgtcatg attttgatgt tggtcgtttt tgaaccagga
     4021 attttttcaa tcgtggctgc tatggccatc acaaactgga taccaatggc acgtatcgtt
     4081 cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt tagctggatt gacattaggt
     4141 gaatcaagaa tgaaaattgc attcaaacat attttgccga atatctctag tgtcattatt
     4201 attcaaatga tgtttagtat tccatctgct attttcttcg aagccttttt aagctttatc
     4261 ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga tgttgagcga agggtacaag
     4321 acattcctgt atctgcctta tctattatgg attccggcag taactttatc ggttatcatg
     4381 attggcttta acttacttgc tgatggattg cgtgatgcct ttgatcctaa aatgaaagag
     4441 tgaagacgat gacgaaaatt ttagaagtga aagacttaga aatctctttt gatacgtatg
     4501 ctggaaaggt acgagcgatt cgcggtgtca attttcattt gaataaagga gaaacattgg
     4561 cgatcgttgg ggaatctgga agtggaaaat cagtgacgac cagaagcatc atgcgcttgc
     4621 tgtcaagcaa cgccaatata gatgctggtc aaatcctctt taaaggtaac gatatcgttg
     4681 gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga aatagcgatg attttccaag
     4741 atccaatgac ctctttagat ccgacgatgc ccattgggaa gcaagtcgct gagtccctta
     4801 gaaagcataa taaagtttca aaaaaagagg gacaaaaagc tgcattagaa ttgcttaaat
     4861 tagttggaat acccgaagct gagaaacgta tcaacagtta tccgcatcag ttctcaggag
     4921 gtcagcgtca acggatcgtg atcgcgattg cactggtatg ttatccggaa atcctgattg
     4981 ctgatgaacc tacaacagcg cttgatgtga ctattcaagc acagattttg gagctgctga
     5041 aagaaattca gacaaaaatc gatacatcaa ttatctttat cactcatgac cttggtgtag
     5101 tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt gaagttggaa
     5161 catcggaaga aattttctac aacccgcaac atccctatac ttggggactg cttggttcaa
     5221 tgccgacatt agatagtgcg aacgatcgat tgtatgctat tcctggttct ccaccggatt
     5281 tattgaaccc gccaaaagga gacgcattct atccaagaaa tgaattcgcg atgaaaatcg
     5341 atgcagaaca agaaccacca ttttttgaac tatcaaaaac acacaaggca gcaacttggc
     5401 tacttgctcc gcaggcaccc aaagtgacac cgccagaaga aatccaacgt cgctgggcta
     5461 tctttaaaga gaaacaaaaa catacttacg gtcaaggagg aattgctgat gccaaataaa
     5521 ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa tgaagtacat
     5581 gctgtaaatg acatcagctt tcacatttat gaaggagaaa cattcggttt agttggagaa
     5641 tctggaagcg gaaaatccac tactggccgt acgatcattc gcttgaatga acctactgat
     5701 ggtgagatat tattcgacgg tcaagatata acgaagatca aagataaaaa aggtttgacg
     5761 aaattccggc atgatgttca gatgatcttt caagatccat acgcttcgtt gaatcctcgt
     5821 atgaaagtca gagatatcat tgccgaagga attgatgtta atggactagc taaatcatct
     5881 gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg ttggattgaa tccaagccac
     5941 ggtactcgtt atcctcatga attttcaggt gggcaacgcc agcgtattgg tattgcacga
     6001 gcattggcag taaaaccgcg ttttatcatt tgtgatgaac caatttctgc actagatgtg
     6061 tcgatccaag cccaagtggt caatttgttg caagatctcc aaaaagaaca tcaattgacc
     6121 tatctattta ttgcacatga tttgtcgatg gttaaacata ttagtgaccg aatcggtgta
     6181 atgcataacg gcttgttatt agagatggga acaagtgatg aaatctataa tcacggtgtt
     6241 catccttata cagaaagttt gttatctgct attcctctac cagatccaga ccatgagaga
     6301 aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg gacaagtacg tcaacttcgt
     6361 gaaatcgcac cagaacattt tgtctatgca actgagcagg aagtcccata ttacgcaaaa
     6421 aaactaaaac gacaaaagga atctcttctg gttgctaact aggagggaca tagatgctaa
     6481 aagcttttaa attccgtatg tatccgacag aagaacaaaa acagcaattg attcgtactt
     6541 ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa agttggcaac
     6601 aaacaggagt agccaatttt tcgctcacac ccgctacatt aaaaaaagaa tacccattct
     6661 taaaagaagt agacagtctt gctttagcaa atgcacagct taatttggat cgggcttttc
     6721 gcaattattt caagggacgt gccagcttcc caaagcttaa aacgaaaaaa agtatgtggc
     6781 aatcttatac gaccaacaac caaacccgta cgatctatct ggaaaatggc tacttaaagt
     6841 taccaaaaca aaaagaacta atcaagataa atcagcatcg gccagttgaa ggatcgattc
     6901 gctccgctac gatttctgct cgatataatg aagaatttta tgtcgcacta ctttgtgatg
     6961 tttcgccagt aaaaaaagaa tctttagcta aatggatcgg gatcgcctat catccgaaga
     7021 cattgatcca aacatctcga cctctagaag taactttacc aaaatttcac cagacagaag
     7081 aaaagctgca acatgcacag cggaaattga atgtgaaagt ccgctccgct caccatagaa
     7141 agatcaggtt agatcaggca agcaattatc agaaacaaaa gagaaaagta atggatcttt
     7201 atctgaaaca aaagaaccag cgagaagatt atcttgaaca attatctggg aaattggtca
     7261 aacaatatga ctacttattt gtagaatctt ttccaaaaga agaggcacat gctgattttt
     7321 ctattcacga ctggcataaa ttaataacaa aattgcgtta taagtcccaa tggtataata
     7381 aaaaatttct tttgatcaat accgacggag cagaagaaag caattccgta agaaagagcc
     7441 aagtagtaga aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg aatgaaactg
     7501 ataggagcgt tagggttagc cttgttaata aaaagtctta atgacttttg tttctaggaa
     7561 tttttctttt gaagaaaagc tcactaatta tgacaaacca ataaaacaat agcagaaaaa
     7621 gttcgtacga gctttttctg ctattgttaa ttttttattc agaaagttta cgaatacatc
     7681 aaaattctga aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt ttcttgaatg
     7741 attaggtctg aatcttttgc tcacaacctt attcatggtg ccccggcttg agctgataga
     7801 tttttgaagg acggccgatt cctacaggac gttcaccgat ctcatcgaaa tattggagca
     7861 tagctttctt aaaattggaa tgatcgatcg ttttataatc gatccctaag aatttagcaa
     7921 atacttttcg agcttccgta atcgtgaaat ctttacctaa aacttgtaga acttgcggct
     7981 cgtgttccat tttattgact acacggttga atgctttcaa gatgatttga ctatggtcaa
     8041 aagccaaagt atctttgcca gaagactctc cagtgactaa atcaagcgag atagccactt
     8101 ctccgctaga taaatggatc ttgttactat gacgttctag tgtgaaccag cgtacttcct
     8161 tggcatcatc tccggcgatt agcggttctt ctccaataaa ggctaagtag ctgactgtga
     8221 cgacccagcc tcgcggatct cgattaggcg tactgaatgt atgcagttgc tcaatatttt
     8281 cttttgaaat aaccacacca gtttcttcct ttgtttctct taagacgctt tctccagttg
     8341 actcatcctt ttggacaaag ccaccaggca atgcccatga atttcgataa ggatgccctt
     8401 ttctttggat caacaaaact ttcagctgat cttcttcttt gttatagcac atcagtacga
     8461 tatcgacagt caaagagggc ttgtcatatt ctggacgttc ctgtttttga taccagttaa
     8521 gaaattcttc ttcacttgct ttatgttcat aatataattt ttcttcttct ttagaagcaa
     8581 aacggttcat ctgaccacct ccattgacag taaaagtact ttttacctta ttgtagcaga
     8641 ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt tatcatttaa atatgaaaaa
     8701 taaatggaag tatgagattg aataaaaaga aaagcagata aaacggcaat actttatacc
     8761 gctcttcacg aatggcaaag aagccacaaa taacgatata tacgtcaatg ataaaacgca
     8821 aaatccaagc tgctgtcata aaactgttca aatgcccatg gaggttaaaa taaccataca
     8881 tacgaatata aaaatagctg aatattaaat gtgctaatga aagaaaagca atactaagtg
     8941 taaaaaaaag atgtttcttc tgcatgattg ttccagtccc ttactaagat agatagtctt
     9001 tattatacca cgtgtgaggg gagaaataaa aagccgtttt ttagtagata tgaaacaaaa
     9061 caaaaacccg catttattca acctattcat ccttttaaag gatagattga acgtagatag
     9121 aagtcgttta tccagtcttt ttcattttga aaaaaagcta acagcatgct ttttttatgg
     9181 tatactagca aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc ccgtggcaga
     9241 aaaggaaact ttttatatca ctacgccaat ctattatcca agcggcaaac ttcatatcgg
     9301 caactcgtat actacgattg catgtgacgc aattgctcgt tataaacggt tgatgggctt
     9361 tgatgtgttt tatctcactg gagtggatga acatggtcaa aaaatcgaaa caaaagcaga
     9421 agaactaggt gtaaaaccgc aagaatatgt agataaaatg gcggctgatg tcaagaaatt
     9481 atggaaaacg cttgatatca gttatgataa attcattcgc acgacagatg attaccataa
     9541 agcagctgtt caaaaaatat ttgatcgttt attagaacag ggagatattt acttaggtga
     9601 atatgaaggc tggtactctg tttctgacga agaattcttt acagaaacac aacttgcaga
     9661 agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg gacacgaagt
     9721 agaattggta aaagaagaat catacttctt ccgcatgagc aaatacgcag accgtctatt
     9781 ggaatactat gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa acgaaatgat
     9841 caacaacttt atcaaaccag gattagagga tcttgcagtt tcacgaacaa cttttacttg
     9901 gggagtacca gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg atgcattatc
     9961 caattatatt actgcactag gatatggctc cgaagatgaa agtctattcg aaaaatactg
    10021 gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc tttcatacga tctattggcc
    10081 aatcatgctg atggcccttg atttgcctct acctaaaaaa gtatttgggc atggctggct
    10141 attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc ctgaaatgct
    10201 tgtggaacgt tatggtttag atgcactccg ttattatttg cttcgtgctg ttccgtttgg
    10261 gtcagatggt gtctttacgc cagaagattt tgtttcccgt gtcaattatg atctggcaaa
    10321 tgatctaggg aacttattga atcgaacgat tgctatgatc aacaaatatt gtgatggact
    10381 cgttcctgac tatgcctcac ttgtcactcc atttgatagt gaactgtcta cgacggctgc
    10441 taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg gaattcaata cagcattggc
    10501 tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag aaccatgggt
    10561 attagcaaaa gacgaagaaa agaaagctga attgaatagt gtcatgatcc atttagctga
    10621 atctttgcgt atcgcagcga tattacttca gccaatcatg acagaaacgc cagagaagat
    10681 cttcaatcaa ctaggattgg actctgaaac aatgaatctt gaaggattac actttggcga
    10741 atttccttca ggaacaaaag tagttgcaaa aggtacacca atcttcccac gtttagatat
    10801 ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa caaacgaaga
    10861 tacagtcaaa tgggatccag aagaaacaga acttgtttca acgaaagaaa aacaaatcaa
    10921 atttgatgta tttgaaaaag tagaactaaa agtagcagaa gtcatcaact gtcaaaaagt
    10981 agaaggggca gataagttgc tgcaattccg actagatgct ggagacagcc aagaccgtca
    11041 aatcttgtca ggaatcgctg aattttatcc tgatccaagc gaattgatcg ggaaaaaagt
    11101 cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc aaggaatgat
    11161 cctttctgca gaagcaccag atggctcttt acaagtaatc gaagcaccaa aatccatgcc
    11221 aaatggctca gaaatcgcat agaaattcaa agggggtatg acactagttg tgtcataccc
    11281 cctttttccg aaatttgaga agcaattttc gtaaattcct tcttattact tgaagccaat
    11341 catataatac agaaacagag aactatccaa aataaatcct agaaaaagaa gtacgagctt
    11401 cgctgatttt tgttaattta gaataatact aataggaatc gtaaaaacag aaaaaaagca
    11461 taatgataaa gggtcgggca gattttaatt gattcatctg gtactctttt ttattgtcct
    11521 tacattcata aagtttgaat gcaatgactt tattacatct atatttatat ctaacagtag
    11581 cgttgatgtg ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat
    11641 gtgtgattga aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc
    11701 tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa
    11761 cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc
    11821 gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac
    11881 cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt
    11941 gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc
    12001 tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc
    12061 ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg
    12121 atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca
    12181 acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct
    12241 ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta
    12301 aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa
    12361 agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa
    12421 attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg
    12481 gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg
    12541 gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt
    12601 ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat
    12661 tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg
    12721 aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact
    12781 ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac
    12841 aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt
    12901 tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat
    12961 taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc
    13021 agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt
    13081 tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa
    13141 tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag
    13201 tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg
    13261 gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc
    13321 tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg
    13381 ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga
    13441 caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag
    13501 cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga
    13561 tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg
    13621 aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg
    13681 gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta
    13741 taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag
    13801 agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc
    13861 tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt
    13921 gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc
    13981 catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa
    14041 atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg
    14101 caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct
    14161 ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt
    14221 gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa
    14281 gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt
    14341 catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat
    14401 atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac
    14461 ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa
    14521 gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg
    14581 gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa
    14641 aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat
    14701 gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt
    14761 cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga
    14821 aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac
    14881 tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat
    14941 tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa
    15001 gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa
    15061 aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct
    15121 tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga
    15181 cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg
    15241 tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat
    15301 atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat
    15361 ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag
    15421 aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat
    15481 tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt
    15541 tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag
    15601 atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt
    15661 taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg
    15721 aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta
    15781 tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata
    15841 tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag
    15901 attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa
    15961 aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata
    16021 acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg
    16081 aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg
    16141 cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt
    16201 gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt
    16261 ttcgtttgtt tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg
    16321 ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat
    16381 gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac
    16441 aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc
    16501 ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag
    16561 ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag
    16621 aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg
    16681 gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg
    16741 cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg
    16801 cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat
    16861 tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg
    16921 ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga
    16981 tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta
    17041 atatgcctga aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga
    17101 ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac
    17161 tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt
    17221 cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc
    17281 tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg
    17341 caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt
    17401 tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg
    17461 cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt
    17521 gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg
    17581 gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg
    17641 aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga
    17701 atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag
    17761 aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa
    17821 acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca
    17881 gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc
    17941 tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat
    18001 aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca
    18061 cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct
    18121 aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat
    18181 ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg
    18241 aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata
    18301 tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt
    18361 ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa
    18421 tcagttacag aataattttc tgcattgtca tattttttat atacgttttt cagagccatt
    18481 tctaccataa tacgttccac actttctttt ttagttttat taactattta aagtataaat
    18541 gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat
    18601 gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa
    18661 ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat
    18721 gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa
    18781 catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg
    18841 atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg
    18901 gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa
    18961 ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat
    19021 atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga
    19081 atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat
    19141 aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata
    19201 taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat
    19261 gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat
    19321 aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc
    19381 ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa
    19441 aatgaagaaa caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt
    19501 gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac
    19561 atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc
    19621 agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt
    19681 tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat
    19741 cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt
    19801 gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc
    19861 gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt
    19921 aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa
    19981 aacaagcagt cttcccatcc cagaagactg cttgttttt
//
LOCUS       DAXWLG010000036        19633 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXWLG010000036 DAXWLG010000000
VERSION     DAXWLG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.036"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            6..653
                     /locus_tag="ACGE4T_001049"
     CDS             6..653
                     /locus_tag="ACGE4T_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8386344.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            737..1681
                     /locus_tag="ACGE4T_001050"
     CDS             737..1681
                     /locus_tag="ACGE4T_001050"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFF8386345.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1816..3267
                     /locus_tag="ACGE4T_001051"
     CDS             1816..3267
                     /locus_tag="ACGE4T_001051"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFF8386346.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3288..4124
                     /locus_tag="ACGE4T_001052"
     CDS             3288..4124
                     /locus_tag="ACGE4T_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8386347.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4124..4783
                     /locus_tag="ACGE4T_001053"
     CDS             4124..4783
                     /locus_tag="ACGE4T_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8386348.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4909..5598
                     /locus_tag="ACGE4T_001054"
     CDS             4909..5598
                     /locus_tag="ACGE4T_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386349.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5604..6560
                     /locus_tag="ACGE4T_001055"
     CDS             5604..6560
                     /locus_tag="ACGE4T_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFF8386350.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6604..7116
                     /locus_tag="ACGE4T_001056"
     CDS             6604..7116
                     /locus_tag="ACGE4T_001056"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386351.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7372..7920)
                     /locus_tag="ACGE4T_001057"
     CDS             complement(7372..7920)
                     /locus_tag="ACGE4T_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8386352.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8009..9190)
                     /locus_tag="ACGE4T_001058"
     CDS             complement(8009..9190)
                     /locus_tag="ACGE4T_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFF8386353.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9426..10934)
                     /locus_tag="ACGE4T_001059"
     CDS             complement(9426..10934)
                     /locus_tag="ACGE4T_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFF8386354.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11093..11284
                     /locus_tag="ACGE4T_001060"
     CDS             11093..11284
                     /locus_tag="ACGE4T_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386355.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11487..12404
                     /locus_tag="ACGE4T_001061"
     CDS             11487..12404
                     /locus_tag="ACGE4T_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFF8386356.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12397..13470
                     /locus_tag="ACGE4T_001062"
     CDS             12397..13470
                     /locus_tag="ACGE4T_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386357.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13618..14136
                     /locus_tag="ACGE4T_001063"
     CDS             13618..14136
                     /locus_tag="ACGE4T_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386358.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14117..14509
                     /locus_tag="ACGE4T_001064"
     CDS             14117..14509
                     /locus_tag="ACGE4T_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFF8386359.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14585..15520
                     /locus_tag="ACGE4T_001065"
     CDS             14585..15520
                     /locus_tag="ACGE4T_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8386360.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15653..16432
                     /locus_tag="ACGE4T_001066"
     CDS             15653..16432
                     /locus_tag="ACGE4T_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8386361.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16425..16742
                     /locus_tag="ACGE4T_001067"
     CDS             16425..16742
                     /locus_tag="ACGE4T_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8386362.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16986..18596
                     /locus_tag="ACGE4T_001068"
     CDS             16986..18596
                     /locus_tag="ACGE4T_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8386363.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18753..19601
                     /locus_tag="ACGE4T_001069"
     CDS             18753..19601
                     /locus_tag="ACGE4T_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8386364.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6343 a         3511 c         3951 g         5828 t
ORIGIN      
        1 aatcaatgaa aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt
       61 gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct
      121 cttcacaggt atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa
      181 gttctagtga agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat
      241 tgttccaaga taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg
      301 gatcttactt ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg
      361 atgctcaaaa tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa
      421 atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag
      481 cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata
      541 aagatttagg tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag
      601 tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc
      661 ttcggaaaac aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt
      721 tgaaggagcg atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc
      781 tttgatgaat gcatctggtg tccactgcat gactacccag gaattagatg aactggctca
      841 ttcagaagca ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc
      901 cgaaccacgc tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt
      961 aggtttttct tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa
     1021 tcaaccgtta tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga
     1081 gaaaatagaa aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt
     1141 gcctggaaaa ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt
     1201 attcagcatc ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca
     1261 ttttgatcag atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa
     1321 cagtgtgggc aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa
     1381 agaagggttc ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg
     1441 cgctttttat acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac
     1501 aggacaagat gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga
     1561 gcttcataaa gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat
     1621 gtcagaaaaa ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata
     1681 gcaaagagac gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt
     1741 cattcctgtg caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc
     1801 aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa
     1861 tttagttttc aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa
     1921 ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc
     1981 cagtcagaaa aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa
     2041 aaaaagggat ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat
     2101 acatctaatt atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat
     2161 gcgccagagt ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac
     2221 ccttggaacc ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca
     2281 tcaggaatcg ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct
     2341 tcgttttgcg gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat
     2401 gcttggagag gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac
     2461 acggaggctt tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg
     2521 ccagttgagt ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat
     2581 tcaccgatcc actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat
     2641 ttccttggaa aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa
     2701 ctgatcacgt cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt
     2761 gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg
     2821 ttccaatatg gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat
     2881 gaagcagcaa ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg
     2941 actgcattta ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag
     3001 atggaacaca tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt
     3061 gaggagagct tgaagatcac accttataca cagttggcta atctgactgg acaaccagca
     3121 atcagcttgc ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct
     3181 gccagaggaa gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag
     3241 ttccatttac caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa
     3301 gattgtttgc tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg
     3361 ctaatcagga agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga
     3421 cttctggcac actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg
     3481 atgtagattt ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat
     3541 acaacgtgga tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg
     3601 attttggtat cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt
     3661 catttgacag tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc
     3721 ttaaagggaa aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac
     3781 acggagctaa attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag
     3841 caaatggtcg aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg
     3901 ctttaccaga tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg
     3961 gttttttatt gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag
     4021 agatgaaaga agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt
     4081 cagtcaaacc gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcctctaat
     4141 tgatttttcc ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt
     4201 agggattggc ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt
     4261 aggtcttttg gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag
     4321 aggcattccc gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag
     4381 tgccttaacg gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat
     4441 ctatcgagga tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg
     4501 tttttctttc tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt
     4561 accggcactc agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat
     4621 cacagtgcca gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt
     4681 ttctctctat attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt
     4741 gcaacagtat ttagaaaaac gttctatcca tcagtcaatc tgaaaaatag aaatggtgct
     4801 agcgtggctg gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa
     4861 ccatttcatg taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg
     4921 ctaaccatcg aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt
     4981 gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca
     5041 aaatttgatg gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt
     5101 cgctctacag ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa
     5161 ctatatgaag aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg
     5221 tacacggttg aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca
     5281 aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc
     5341 agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa
     5401 ttgtacaaag aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat
     5461 tatgaacggt ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt
     5521 atcgcgcttc agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct
     5581 acagagccat tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc
     5641 tattagcatt aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga
     5701 tcgaaccagt acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc
     5761 accatccaat cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc
     5821 atgtgcacag ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc
     5881 cagaaaatct cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc
     5941 actacccaaa agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga
     6001 taccagatac tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact
     6061 cttttcaaac gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg
     6121 atgactatct ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag
     6181 gtagccgtta tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata
     6241 ttgatgctta cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta
     6301 aagaccaagc attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc
     6361 accatcatca actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac
     6421 aaaaattccc tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta
     6481 ttagtcacct gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac
     6541 tatatgaagt gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac
     6601 tggatgtata ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat
     6661 gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca
     6721 acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa
     6781 gatcaaattg ctggaacagc tgcattagtc tcaggcgtgg atcccgttta cacaaaaata
     6841 gatggtagtt ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca
     6901 gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca
     6961 gccagcaaaa atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa
     7021 aagaccatct attctgctgg attcacttat caagggatga ttgaatttcc ttttgaacac
     7081 ggcgaaagaa aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat
     7141 aaagacatta acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag
     7201 tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat
     7261 tttagcgtat tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca
     7321 tagcttttgt cacgatctct cggcttatcc tacgagtatt attcgataca tttatatcaa
     7381 taatcctttt tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa
     7441 aagtgagggg atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag
     7501 tctggctgaa atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc
     7561 tttagagatc gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca
     7621 aaaaccattt gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc
     7681 tgctttactg gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc
     7741 cttgatcatc tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc
     7801 cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc
     7861 ttctgcgagt gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat
     7921 tgtcatttct cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct
     7981 tttaaaagta actttgcttt tttgtatttt ataccagcaa gccagtcaca atagcactta
     8041 aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc
     8101 ctttttcttc atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg
     8161 caaatgaaac gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac
     8221 catttgctaa ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac
     8281 taccggcatc cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga
     8341 tgtaacctag gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca
     8401 tggcaatcaa agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac
     8461 catcaagaat gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga
     8521 tcagctcatc ttctttttct tctaatacat agggatttac aattgaagcc agaatataac
     8581 cgccaaataa gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat
     8641 aagatccgac gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac
     8701 gtttctcaga taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta
     8761 aaatagcaga agctactcca ttataagatt ccaatttgcc catcccgttg attttactta
     8821 atcctagacc aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga
     8881 taatagcaga aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt
     8941 tatttgctac accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat
     9001 caaacatttt tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga
     9061 aaccaagaac aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt
     9121 tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata
     9181 aataactcaa ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata
     9241 aaatactaat ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc
     9301 attagaaaat aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct
     9361 ataaaaaaac gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac
     9421 tttttctatt ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt
     9481 cctagttttt tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt
     9541 gctccctcaa gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca
     9601 ctgattgata aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt
     9661 gtctttgatt tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca
     9721 tctgcgatat gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc
     9781 aatgaacttg tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca
     9841 gaaggatctt ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact
     9901 accacgtttc ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta
     9961 actgcttctt cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg
    10021 atgatttctt gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca
    10081 cgattcacac ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca
    10141 ataatacgtt tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta
    10201 ttgtaggcat caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct
    10261 gcttcataag caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg
    10321 aacttggagt aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaagcaa
    10381 gtagcgagct ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt
    10441 tcaatatact cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga
    10501 tagtcttcga tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata
    10561 aagactttcg tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag
    10621 ccataaccac gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa
    10681 tcaccacgga tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct
    10741 tgatctttca atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca
    10801 cgtttcgcat gcttgtcgtc aactaacttc ccaccaaatt ctaaatataa tttgtcatag
    10861 tggttcactc gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa
    10921 ccaatttttt tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg
    10981 attcgtcaac tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact
    11041 tgtctagata gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa
    11101 aagacagaaa aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga
    11161 aagaatgctt gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac
    11221 gcagctaact cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa
    11281 ataaaaaata agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt
    11341 tgatcgaaaa aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc
    11401 tcttttttat aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa
    11461 cagatgagta agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc
    11521 cagacgaact agaggagacg aacgcggatc aggatacagg acaagaatcg tcttcttttt
    11581 acgatatcaa agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt
    11641 ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat
    11701 tgaatgaaga attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc
    11761 gtaagatcca tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct
    11821 cgccaacgat gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta
    11881 ttgatgcaag tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat
    11941 attcccaacc ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga
    12001 tcactgctca gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa
    12061 tgactgattt gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc
    12121 aagaattaga gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata
    12181 ttgagacgcc tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag
    12241 accagcttcg tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta
    12301 gcaaagaggg attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga
    12361 atcctaataa tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa
    12421 tggacaaaaa atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa
    12481 caaacaagta atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata
    12541 catgaaaaag aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac
    12601 gaaataactg aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc
    12661 agtttgattg aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc
    12721 gaatcagatt ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca
    12781 atctccacga tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg
    12841 ttgaatgata ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat
    12901 ttttcgatca attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg
    12961 accatcgatc aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca
    13021 atggaaatca atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat
    13081 cgagtattct ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc
    13141 catgcaagaa cacttggttt taccgttaat cagacattac tagacaatac gaagccactt
    13201 cgtttgaatc ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc
    13261 gtggcaatga aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta
    13321 cgattagata aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg
    13381 tcgatctcat ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc
    13441 ttggtcccat taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc
    13501 agataattct gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt
    13561 ctgtatgtac taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg
    13621 tccacaaata aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc
    13681 gcttttcaat ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt
    13741 tacttaagct taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt
    13801 tattttggtg ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt
    13861 ttgttttcaa tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc
    13921 tgtttttttc tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta
    13981 tatcgtttgc ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac
    14041 agtataggca atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat
    14101 cattcaggag gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat
    14161 actcattttg acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga
    14221 agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga
    14281 aaaagaagtg gtcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga
    14341 agtcaaagaa gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa
    14401 tcaactctat tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat
    14461 caaattgaat gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa
    14521 aactgttaaa gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa
    14581 taacatggaa aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa
    14641 tattctagtt gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct
    14701 aaacgaaagc atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga
    14761 taaacaagcc aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata
    14821 ctttttcagt acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat
    14881 ggaagctttt gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat
    14941 agcaatactg ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga
    15001 aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca
    15061 cagtgaaata ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc
    15121 tttagcagga acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg
    15181 attgctgatc ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag
    15241 tctgatcatc ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga
    15301 acgtcctgag atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat
    15361 tttagttgca gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat
    15421 caatgatatt gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat
    15481 tgaaacagat aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga
    15541 agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt
    15601 tatgttacaa tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa
    15661 aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta
    15721 tataccagta ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt
    15781 atcaggactg ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat
    15841 gcttacgacg caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg
    15901 ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt
    15961 ggctgtcttt tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc
    16021 aactgaccct tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc
    16081 atgggccggt agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga
    16141 ccttgtccat tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag
    16201 cgcactactg atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt
    16261 attccaaaat actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat
    16321 gctggagaag gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt
    16381 caatgagccg ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt
    16441 tgctactttt agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc
    16501 tttattttac tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg
    16561 tggcattatt tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata
    16621 ttgcttggaa tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac
    16681 gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt
    16741 aaactgtttt attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc
    16801 cgaactgcat ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat
    16861 tagtgttggc ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct
    16921 tctatgattt tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa
    16981 tttaaatgaa taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat
    17041 gtaaagtttt aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag
    17101 atggtatgct tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga
    17161 tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag
    17221 aaggagataa cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca
    17281 tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc
    17341 cgattgctaa cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg
    17401 ctatcccaat tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt
    17461 atggaacttc tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt
    17521 attacttgcg cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg
    17581 cttctttctt cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag
    17641 acttcttcga acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat
    17701 ctgcctctgt ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta
    17761 ctatcgttca aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag
    17821 agatcgcaga tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat
    17881 tgacattgat cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa
    17941 gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga
    18001 ttgcgttatt gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat
    18061 acacattgtt ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag
    18121 cttctttgtt tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc
    18181 atcgttttgc gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa
    18241 tcggattagg attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta
    18301 ttgtctttgt tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa
    18361 agatcagcaa ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt
    18421 ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga
    18481 tttattgtgt ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa
    18541 aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta
    18601 actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt
    18661 ttctattttc caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg
    18721 gaaaaattct ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt
    18781 cggcatatct gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt
    18841 tgattatgga tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg
    18901 gacttttaaa aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt
    18961 tgggtttata tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt
    19021 cttcagggat ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag
    19081 taacggtcat tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag
    19141 ctgccttaga cttaaatgat gaaggtcagt cccttgatga aatacttgca catatcgatc
    19201 gaatccgaga taatacctat gcttatctga ttgtagatga tttgaacaat ttagtacgag
    19261 gaggacgttt gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc
    19321 tgacattttc tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat
    19381 ttgctcgagc tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac
    19441 tttacgtgat acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga
    19501 aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt
    19561 taggtgaaaa agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac
    19621 ataccaaaaa tat
//
LOCUS       DAXWLG010000037        19461 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXWLG010000037 DAXWLG010000000
VERSION     DAXWLG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.037"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            80..2098
                     /locus_tag="ACGE4T_001070"
     CDS             80..2098
                     /locus_tag="ACGE4T_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8386365.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2168..2374
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2525..4939
                     /gene="leuS"
                     /locus_tag="ACGE4T_001071"
     CDS             2525..4939
                     /gene="leuS"
                     /locus_tag="ACGE4T_001071"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFF8386366.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5287..6840
                     /locus_tag="ACGE4T_001072"
     CDS             5287..6840
                     /locus_tag="ACGE4T_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFF8386367.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7221..9005)
                     /locus_tag="ACGE4T_001073"
     CDS             complement(7221..9005)
                     /locus_tag="ACGE4T_001073"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386368.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(9020..10741)
                     /locus_tag="ACGE4T_001074"
     CDS             complement(9020..10741)
                     /locus_tag="ACGE4T_001074"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386369.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10841..11602
                     /locus_tag="ACGE4T_001075"
     CDS             10841..11602
                     /locus_tag="ACGE4T_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8386370.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            complement(12001..13179)
                     /locus_tag="ACGE4T_001076"
     CDS             complement(12001..13179)
                     /locus_tag="ACGE4T_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFF8386371.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGAKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            13382..13606
                     /locus_tag="ACGE4T_001077"
     CDS             13382..13606
                     /locus_tag="ACGE4T_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFF8386372.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            13626..13913
                     /locus_tag="ACGE4T_001078"
     CDS             13626..13913
                     /locus_tag="ACGE4T_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8386373.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            14373..14753
                     /locus_tag="ACGE4T_001079"
     CDS             14373..14753
                     /locus_tag="ACGE4T_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFF8386374.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            14735..15649
                     /locus_tag="ACGE4T_001080"
     CDS             14735..15649
                     /locus_tag="ACGE4T_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFF8386375.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            15609..15887
                     /locus_tag="ACGE4T_001081"
     CDS             15609..15887
                     /locus_tag="ACGE4T_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386376.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            15894..16583
                     /locus_tag="ACGE4T_001082"
     CDS             15894..16583
                     /locus_tag="ACGE4T_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386377.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(16704..16790)
                     /locus_tag="ACGE4T_001083"
     CDS             complement(16704..16790)
                     /locus_tag="ACGE4T_001083"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8386378.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            17427..18371
                     /locus_tag="ACGE4T_001084"
     CDS             17427..18371
                     /locus_tag="ACGE4T_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8386379.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            18392..18925
                     /locus_tag="ACGE4T_001085"
     CDS             18392..18925
                     /locus_tag="ACGE4T_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFF8386380.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            18907..19110
                     /locus_tag="ACGE4T_001086"
     CDS             18907..19110
                     /locus_tag="ACGE4T_001086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386381.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
BASE COUNT         6628 a         3461 c         3835 g         5537 t
ORIGIN      
        1 gctcttaaat aacttttaat aaatattaag agaagttata attattaata atagataaat
       61 tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac gtttagaaca
      121 gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg cgatggtagg
      181 ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg aaacacctgt
      241 tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta cttatgctca
      301 accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag cagttgtaga
      361 aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt ttgggataaa
      421 aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa ataatcaatg
      481 ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt tcaatgacaa
      541 tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg gtacttggaa
      601 gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt atgcgactga
      661 tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga cccaatatga
      721 tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata atactggaag
      781 tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt cagcaacttc
      841 gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata aattcggtat
      901 cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt atccaggaca
      961 aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt ccggtaacaa
     1021 taccaactct tcaggaaatg ctggtacatc aaacggagga caaacaactg gtgcgaaata
     1081 cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa ttacgatggc
     1141 tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac aacagctaat
     1201 cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata ctgggaatac
     1261 aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa cgaaatatac
     1321 agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta cgatggctca
     1381 gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac aactgactgt
     1441 ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa ccaatacaaa
     1501 taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata ccgtgaaagc
     1561 aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc agttaatcga
     1621 atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg ttaaaggtgg
     1681 aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa atacaccgaa
     1741 tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg tgaaagcggg
     1801 cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt taatcgaatg
     1861 gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga agaaaggttc
     1921 atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg tgaaatctgg
     1981 agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac taatcgaatg
     2041 gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag ctaaataaaa
     2101 tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta taaggctact
     2161 taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag agagcgcatg
     2221 gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct tgctgaatac
     2281 ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc actaaggtaa
     2341 aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt ataggatttt
     2401 tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat aaacagattc
     2461 tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa caggaggaca
     2521 gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact gggcaaaaac
     2581 gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac tggatatgtt
     2641 tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata cggcaacgga
     2701 tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa tggggtggga
     2761 tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc cagctgagtt
     2821 tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat ttagttatga
     2881 ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac aatggatctt
     2941 tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa actgggtacc
     3001 tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg aacgtggtgg
     3061 ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg cttatgctga
     3121 tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag aaatgcaaag
     3181 aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg gtacggataa
     3241 atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt actcggttct
     3301 tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag ctgctgtaga
     3361 agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc ttgcaaaaga
     3421 aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca aggaaatccc
     3481 aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca tggctgttcc
     3541 agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa ttcttccagt
     3601 tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc acatcaactc
     3661 tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg aatggctaga
     3721 agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc tattctcccg
     3781 tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa cagtcacagc
     3841 tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca agccaagcgg
     3901 tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg atcctgtaac
     3961 aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa gctcatggta
     4021 ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg aaaagttgaa
     4081 gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac ttcatttact
     4141 atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta caaaagaacc
     4201 attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa aaatgtcaaa
     4261 atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg cagatacact
     4321 gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga gcgaaaatgg
     4381 cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg atgagaacaa
     4441 taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag tgtatcatca
     4501 aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag caatctccca
     4561 attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg aatatatcga
     4621 aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac tatgggcaat
     4681 ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg aagcagcact
     4741 tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg cgaaaagcaa
     4801 tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca atgaaatagt
     4861 gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa acaagttagt
     4921 caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt gtgcaaaatc
     4981 aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc aaattgatta
     5041 attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat gtctttttct
     5101 tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt aatcttagta
     5161 ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata aattttttat
     5221 tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg agaaaaggag
     5281 agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca aaaaattatc
     5341 gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt agtatttttc
     5401 tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag tgcagtagtc
     5461 cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg gggagttggc
     5521 ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg ttaccatcaa
     5581 ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt acataataaa
     5641 ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag tcaagctttt
     5701 cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt acctatctac
     5761 ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt actagaaaac
     5821 gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac taagatgagt
     5881 acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta tgggagatac
     5941 actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat gaactcaact
     6001 aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa gcaaaaggta
     6061 aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca ctctctctat
     6121 atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca atggttgcgt
     6181 tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat ggacaacaca
     6241 tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga tgtacgtctg
     6301 ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca atcagttcag
     6361 ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat ctgtacatca
     6421 gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg cggaatcact
     6481 tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag cttcgttaca
     6541 acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa cggaggacgc
     6601 tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg gatagatctt
     6661 tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac aaaattttat
     6721 ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt ccgttcaact
     6781 gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca aggagattaa
     6841 agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa ttcaaaaaac
     6901 agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag agcaacaaga
     6961 agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg ccgataggtt
     7021 gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg tcactactta
     7081 ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt tgtgtttcta
     7141 gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata catgatcgat
     7201 agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag actggcataa
     7261 agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc gccattcttc
     7321 attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat cacaaaactt
     7381 gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt tcgtgtatct
     7441 acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat gactcgagca
     7501 attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga aatgatcgta
     7561 tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc agccgcttcg
     7621 ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc taccgttcct
     7681 tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc tccacgtgaa
     7741 agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc ataaaaacgc
     7801 atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat cgctaccgtt
     7861 tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga tgtgtaacca
     7921 aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct tgcatctgga
     7981 aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat tgctgccatt
     8041 gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga aatctgattg
     8101 atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag aacaagcata
     8161 gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat ggccggatag
     8221 atagcacagt tcataaattg agcacgttta aaagcgttgt attgcttttt atttacttcg
     8281 tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag attaccagct
     8341 aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg ttcagaaatc
     8401 tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat cagcaataag
     8461 acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc agctattgtt
     8521 acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag ttgattgaga
     8581 ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa cgacatcggt
     8641 aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt ttcgcttaac
     8701 gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga acagccgatc
     8761 agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag ctgtccaata
     8821 cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat caagggcatg
     8881 gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa atatttatgt
     8941 tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt ttttttcttt
     9001 tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga taagtttggc
     9061 aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca tctaaaacaa
     9121 tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg agagttttct
     9181 ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag tctaaagcgg
     9241 agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga gcaatacata
     9301 tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag ctgtccaatc
     9361 cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct ttagacaact
     9421 cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc ccgctgaata
     9481 agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga gtcatttgag
     9541 taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt ctcaagagaa
     9601 gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt gtttttcctt
     9661 taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta tatttaaatg
     9721 tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct actgttttga
     9781 tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt cgtaatgagg
     9841 caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa gcaagtgtca
     9901 gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct tctgctacaa
     9961 gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa aaagcaattg
    10021 gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag tcagcaaagc
    10081 tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc atattaatcc
    10141 cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg atactcaaag
    10201 ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa acaacagaga
    10261 tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg gtgaaaacgc
    10321 aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc tggacattat
    10381 cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg atatccttca
    10441 ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca aacctagcgg
    10501 aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga aacgctacaa
    10561 gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct atacctgtgt
    10621 cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttct aaacagatca
    10681 tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt aagatagaca
    10741 tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta acttgaaggt
    10801 caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa tgtatacgat
    10861 tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt gggatgaaca
    10921 aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg atatcgatga
    10981 tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga tcaaacagat
    11041 gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg aaacagaaag
    11101 aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa gggagcaaaa
    11161 aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag aagagatccc
    11221 ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa aatttctacc
    11281 taactattca agctttggga tgatgagtat gccagcttcc agttcacaga cgatgtatgg
    11341 attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt gggagaaaaa
    11401 agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg cggaacgtaa
    11461 taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag gagaagtgat
    11521 cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat attatcatgc
    11581 atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa tgatggttgt
    11641 tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg gattattagt
    11701 cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat ggaatggttg
    11761 caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga aaaataattg
    11821 tattgggtgt tatgtataat tcatggtgaa ctgagactaa tgagaagaaa aattcatatt
    11881 gttatgagtg agattgtatc agatgagaat gataaattaa ataggtgcat aaaagcatta
    11941 cacttaaagg gagcgtcaat aattttgtgt aaataactcg tccttctgca aaataatggg
    12001 ttactcagta aacattgaag ctaatgtatc cgtcacttgt tggaagcctt tatggcttct
    12061 atttaagaat ttttgattgt atgtatcaaa gatggatact aggaaacgct ctagtgattc
    12121 ttcattttga aactgctctt ttctgcggct gtatttcttg atttgtttat tgaaagattc
    12181 gattaaatta gttgagtaaa tgcttcggcg gataccaggc ggaaactcat aaaaagtcaa
    12241 taaatcttga ttttttatca gtgactgcgt cactttagga taagttttct gccacttctc
    12301 aatcatactc cctaaaaagg tatttgcctc ttcctttgag ctagcttgat aaacagcctt
    12361 aaaatcatca cagatttctt ttcgatcttt gacacgtact ttatgagcaa tattacggga
    12421 aacgtgaaca cagcaatgtt ggtatttagc tttaggataa atctggtgaa tcgtatcttt
    12481 catgcctttt aagccatcgg taataaacag taagacatca tgaacccctc tggagtaaat
    12541 gtcctgtaac aactcattcc aaacgtaggt cgattcagtc ggagcaatcg cataacttag
    12601 tacttcttta gcgccgtctt ctcgtatacc aatggcaata tagattgctt ctttggagac
    12661 ggtttgccgt tttaaaggaa tataagtagc atccatgaaa atagcgacat acttatcatt
    12721 tagagttcta actttaaatg catttacttc ttcagtcagc actttagtca tgttggacat
    12781 ggtttggggc gtgtagtgat gcccatacat tttctcaatc aagtctgcga tttccgacat
    12841 ggtaatacct tttcggaaga ggtgaatgac ggtctcctct aaggtatcat tcgtccgtct
    12901 ataagcagga acagtctgtt gcttgaactc gccgttgcgg tcgcgcggaa tctggagatg
    12961 aagttccccg tactcggtct taaccgtacg gtcataggag ccgttacgag aattacccgt
    13021 gttaaaacca atacgatcgt acttttcgta atcgaggaaa gccgttaact ccgttttgag
    13081 aagcgtattg acagcttttt ccaaatggac acggaataat tcattcaaat cgcctttgtt
    13141 cgctagagtc tttagaattt ctgtagtaaa atcgttcata tggaagtcct cttttctgtg
    13201 aatgtgttgt ggtaactcta ttctacagaa gggacttcct tttttctatt tacacaaaat
    13261 attttacact ctccttttta tgacgttaat cattgttata taataaaatg ttatataata
    13321 aaattactaa gattatttaa tataacaagc aattttaaaa aaacgaaaag gaaaaacaat
    13381 catgaaaaag aaagtattaa aacattgtgt tattctagga atattaggaa cttgtctagc
    13441 tggcatcggt acaggaataa aagttgatgc agctacttac tatggaaatg gtctttattg
    13501 taacaaagaa aaatgttggg tagattggaa tcaagctaaa ggagaaattg gaaaaattat
    13561 tgttaatggt tgggttaatc atggtccatg ggcacctaga aggtagtggt agggaggtta
    13621 ttataatgga ttttactaaa gaagaaaaac ttttaaatgc aattagtaaa gtatacaatg
    13681 aagcaactat agatgactat cctgacttaa aagaaaagct ctttctttat tctaaagaaa
    13741 tcagtgaggg aaaaagtgtt ggtgaagtta gtatgaaatt aagtagtttt cttggaagat
    13801 atattttaaa acataaattt ggattaccta aatctttaat agaattacaa gaaattgtta
    13861 gtaaggaatc tcaagtatat agaggatggg cttctattgg tatttggagt taattctttg
    13921 gaataaaatg aaaatgggac tgaagaatca attctgattc atcagtccca taaattatag
    13981 agccgaaaaa ttatcatatt tatagattta ttaatagtaa atggcataag aatctttgaa
    14041 caacgaaaaa aatgtagctc tacctaatta ttgatttgca acaatttcac cgaaggtgaa
    14101 acaagcgaaa ctttacaaaa agtggctagc atttgctagt cttttttgta ttgtggagag
    14161 tgggggggag ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcacc
    14221 aaatacgtag gtatttggct agcaaatata gagcttgcca agccctttag tgagctttag
    14281 tgagctttag tgagctttac ttattgtacc tttttaggta caatggtatt gcgaacgata
    14341 cgtccgtagg acgtactgaa agggtgggac ttatgagcga acaaaatcaa tatttggcta
    14401 gcaatcattc taaaaaatat acctatcgat ccgagccaaa acaaatcagt tttcgagtga
    14461 gcgaatccga atttgcaaag ctgaagcagt cagctgaagc tttgcaaatg agtgtgccgg
    14521 ctttcgtgaa agccaaggca caaaacgcgc gtctggtgac accgaaagtg gcgccagaca
    14581 ttgctcaggc aatcgcgcgc gatttagcca aagctggcgg gaatatcaat caaattgcca
    14641 agtggtgcaa cacacaccaa cacgacgttg atcctggcga cgcccaacgc ttatccgaaa
    14701 acctaaaaat catgcaaaag gagctacaaa agatatggca acagttaaag taagccgaac
    14761 cacttcttgt agccgagcga tcaattacgc cgaaccacga gcaaccgtca aaacagggat
    14821 aaactgtgac attgattatg ccaaaagcga aatgaaacaa attcgcatgc tgtatggaaa
    14881 agacgatcgt gtacaagcac acttattgat ccagtctttt cgaccaggag aaattaccgc
    14941 tgaaaaagcc aatcaactag gggcagagta tgcgaaaaaa atagcccctg accaccaaat
    15001 cgcgatttac acacatacag acaaagacca tattcataac cacattgtca ttaactccgt
    15061 caatctggaa actgggaaca aatttcaagc ccatggtcaa gcatttttaa acaaatgtta
    15121 tgacctaaat gacgagattt gtcttgaaca tgacttaaca atcactgaac gagggaagaa
    15181 acccgaaaaa cgaaccatgt ctgaaatcaa attaaaagaa aaaaatgaac ctgtttggaa
    15241 agacgaaatt cggtttgcga ttgatcaaac aatgaaaaac aaaaaaactc gaacgtataa
    15301 ccaattttgt gattctctta aaacattcgg cattcattgc ttgaatcgtg ggaaaaattt
    15361 tacttacgaa ttgatccaca aaaagaaaaa agtacgatcc aacaaactag gaaaggatta
    15421 cgaaaaggag acgattttac atgagctgga cagacgagaa cgagcctaca atcaacaacg
    15481 aaccaacgag ctacgaagag ctcgagctag cattcgcgaa actgcaaaag aagtacgaag
    15541 agaacaacga actcttacga ctgaacaacc ggaaattaga ccgactcgaa gaaaccatga
    15601 ccaacacgtt gaaagaggag cagactacca acaagaatta tcgttctaaa acgttacaag
    15661 acatagaaaa tgaaataaag aaacaacgtg cttttcttgc accttatacc aaagaaagaa
    15721 tacaaactac aattgattca caagtcaaaa aattccacca tgcgctatta aaccagcgaa
    15781 ataaagaaca gaaaaaagta attatgcagc gatcaatgga tattcttgcc cccttatgtt
    15841 gcttaatcat gacactgtta gccttttggc tggtatgggg agaataaata gaaatggcat
    15901 tctatttacc ctatctactc atttttgcca gtattttagg aattatttgg cttagctata
    15961 aggcttatca aacaagatat tcattaaaag gctctaaaat tcgatttaag aggtttttat
    16021 tgccaggggt tatatttaca ttatcaatcg ttttaagtgg tttattcggc gttttagagg
    16081 gaaataaacg tatttcaaga agtattttat taggggatat cactcaaaag tatgaagcag
    16141 catgtgataa aaagaaaaag gagcaagtgt taacacaaaa aatggaagag tttaccactt
    16201 gttatgagga aatgaacgat atttttgtga atcaagaaaa acgtttgacg gataaaaaca
    16261 tggaaaaact cactcgtctt tatcaaaact tgccagaaaa acagcaaaaa gaggttcaag
    16321 acaactatga gcgaaccaaa aaagacgtgc aatattttaa agatacgcag acagaagaag
    16381 cttgttataa tttattcagt gatacaatcc ctttttcaac tagtgaacag gaacgaaaag
    16441 aacggcaaca atcagttacc tacgaacgtt ataaagcact gcttcagcaa gcaacgaaca
    16501 tacaaaatcc aacgaaaaaa gaaacggcat taaattatct aaaaagtgtc aaggaatggt
    16561 tagatcagca acagcaaaat tgaatttgat cggcaatcat gttaaactaa acatgcaaat
    16621 gatcaaacat tagatacacg aaagccccag agaggtcgga ttctctgggg ctttttttca
    16681 ggttgatgtc gcctttattg attttacttc ttgtttcggt tattcaacca ataactgaat
    16741 aaagagacac aaacttctat aagaaggggg caaatgaaca attcaaacat tagatacacc
    16801 tccttccagt cgcaagactg tcggataggc gacaagttat tatatcttaa aaaattaaac
    16861 ttgtgtggtg tttttaagta tcgcttattt tagggttgtt agggcttgcc ctgaccgcct
    16921 gtaaggcgct ttaaagctag ataaggattt ttatccaaga ctgtcttaaa acggcttaga
    16981 taagcattca gaaccgctca aaggcctctt taccaacatg tttttagcaa aggagccttt
    17041 aggcgactgc gcagtacaat gtgagagcgt agcgatcaca cctttttggc gaccgtagga
    17101 agcaaggttt tggattggca gttttgaaaa atgcgcgaac ctgtttaaag aaaaaagaag
    17161 tggtttttag gcgggaaaat tagcccccct ctttttatat ctctttttaa acctcttttt
    17221 aaacctcttt accgtaagct gtgtccttac tctaccaatg gatacagaat gttatgaagg
    17281 acattttgtc tgtttatgaa ggacattttg tctgtttatg aaggacattt tgtctgttta
    17341 tgaaggacat tttgtctgtt tgaacgtcaa atatgaatgt cttgacattt aaaaaatgtc
    17401 attcataatt ggattgaggt gattatatgg ctaatgagat tgtaaaatac caccatgaat
    17461 taaatacaat tcctttaaga aaatttacac cggttgaaat gaatttgttt ttttccatcg
    17521 tgtctagaat gcgtgaaaaa ggggataaga cagttcgttt tacgtttgac caactaaaag
    17581 atttaagtaa ctacaaagca actgcaaatg tacgatttgt agaagattta aaagcaactt
    17641 atgaaaaaat cttatcttta cgctttggtc gtagaagtga gtctgggctt tcttacgata
    17701 tgtttgtaat gtttacagaa tttaaaatca acggggacgt agaaaatcct tacgtagata
    17761 tacgaattta cgaaaaagca ctgcctttgt taaataattt agacgagtgg gtacgctatt
    17821 ctttacaaca attcaacgag ctacaaagtg gctatgcaaa gactatgttt cgtctgttaa
    17881 agcaatacag aaccaaaggt ttcgcatatt tctccaaaga agactttctt gaattattag
    17941 atattcctaa aagttataag caacctgata tagataaacg agttataaaa cctatacgtc
    18001 aagaactaac tgcaatcttc aaaggactaa caatcaaaaa gaaatacgga aaaggacggg
    18061 gaaaacctgt tattggctat caatttacgt tcaaacctga gatcaaaaaa gcagacgact
    18121 ttaacaaaca tgcaccaacc aaagaagacc aaaataaagc attggcttta gctgataaac
    18181 aaaaagaata cgaagaaccc gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag
    18241 aacttgaact cctccaacaa atcaaacaac tagaaaaaga gaaaaaagct cttgaaaaac
    18301 aaacagagtc cgcactttcg gaaaaagaga aaaaaagttt attatctgat ttagataatt
    18361 tattccagta gcaacctagg aggcaaccag tatgcaacca caagaaacct ttacaataaa
    18421 ggaattgtca gatcttttta agatgagtag gcaagcaata tcgaaacata tccaaaaatt
    18481 agattcttcc atgatagcta aaaatgagcg tggttataag gttgtattgc gttctggagt
    18541 attgcaactt gctcgcaact tagacaatca aaggttgcta gataagttgc ataaacatga
    18601 acaaaacaac gataaaaatg cttatatacc aaacaaaaac gtggttgcaa acaagttgct
    18661 agatcagttg cgtgaagaaa atgaattttt acgagaacaa cttgccctca aagagcaaaa
    18721 taattctcat atacaaaaac tactcgacca gcaacaacaa ctcaccctgc aagccaacca
    18781 acaaatcgaa aaacttcagg agcaactgca gttgacttat acggaagagt cttcagagaa
    18841 tagttcaacg actctttctg aagcaacaga aacaatggac gaacaaacaa agaattggaa
    18901 aaaatggtgg cagctatgga aatgaagaag gaaatactta tgacaaaaaa acacttgcta
    18961 ttcggttcta tcctactagg aatattaagc tttagcccaa tgttattatc agtggccaac
    19021 aaaggaaata aaaaagggga actaaaactc aatcctaatt actacaacag tcaaaatgag
    19081 gaagtatttg tagaaaaact tgataaataa gaaaagactg ctcgtacgag cagtctttct
    19141 gaaagagatt atcctattat taaccttaga aaagaataat aaatgaatat gagatatgta
    19201 aaaagcataa caaacacaaa caaaaataac aagtatttaa aaaatgaccg atcaatattc
    19261 acaaagaatt agaaagaaac aaccgcagaa aaccaagcct taaaaagagc ttttaaccta
    19321 gaccaataaa aattcacaaa atcagcgcct ggatcccttg gtacaggcgt tacttggtag
    19381 tatacacatt atgtctacta gctgtttaaa taactgatct tataatttta tttaaacgat
    19441 tatgaattca aaactacttt t
//
LOCUS       DAXWLG010000038        19444 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXWLG010000038 DAXWLG010000000
VERSION     DAXWLG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.038"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            125..3652
                     /locus_tag="ACGE4T_001087"
     CDS             125..3652
                     /locus_tag="ACGE4T_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFF8386382.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3649..7371
                     /gene="addA"
                     /locus_tag="ACGE4T_001088"
     CDS             3649..7371
                     /gene="addA"
                     /locus_tag="ACGE4T_001088"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFF8386383.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7440..7781
                     /locus_tag="ACGE4T_001089"
     CDS             7440..7781
                     /locus_tag="ACGE4T_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8386384.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8090..9133
                     /gene="pheS"
                     /locus_tag="ACGE4T_001090"
     CDS             8090..9133
                     /gene="pheS"
                     /locus_tag="ACGE4T_001090"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFF8386385.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9138..11558
                     /gene="pheT"
                     /locus_tag="ACGE4T_001091"
     CDS             9138..11558
                     /gene="pheT"
                     /locus_tag="ACGE4T_001091"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFF8386386.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11728..12738)
                     /gene="trpS"
                     /locus_tag="ACGE4T_001092"
     CDS             complement(11728..12738)
                     /gene="trpS"
                     /locus_tag="ACGE4T_001092"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFF8386387.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12992..13729
                     /locus_tag="ACGE4T_001093"
     CDS             12992..13729
                     /locus_tag="ACGE4T_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8386388.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13743..14558
                     /locus_tag="ACGE4T_001094"
     CDS             13743..14558
                     /locus_tag="ACGE4T_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8386389.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14571..15218
                     /locus_tag="ACGE4T_001095"
     CDS             14571..15218
                     /locus_tag="ACGE4T_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8386390.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15234..15890
                     /locus_tag="ACGE4T_001096"
     CDS             15234..15890
                     /locus_tag="ACGE4T_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8386391.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16052..16873
                     /gene="racE"
                     /locus_tag="ACGE4T_001097"
     CDS             16052..16873
                     /gene="racE"
                     /locus_tag="ACGE4T_001097"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFF8386392.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16876..18231
                     /gene="rph"
                     /locus_tag="ACGE4T_001098"
     CDS             16876..18231
                     /gene="rph"
                     /locus_tag="ACGE4T_001098"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFF8386393.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18232..18750
                     /locus_tag="ACGE4T_001099"
     CDS             18232..18750
                     /locus_tag="ACGE4T_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8386394.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18857..19357
                     /gene="cbpB"
                     /locus_tag="ACGE4T_001100"
     CDS             18857..19357
                     /gene="cbpB"
                     /locus_tag="ACGE4T_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFF8386395.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6537 a         3340 c         4174 g         5393 t
ORIGIN      
        1 tatacttaaa aaagtagaaa tatcagaacc ttaaaaggat ataatttgaa gaatatagaa
       61 caaatgttca gtattttttg atataataaa ggagcaccgt atcatgtgtt gaaggaggca
      121 aagcatgtct ttacaattta ttcttggaaa cgggaaaaaa gatcaccgtc aagtcttgat
      181 cgacgaagca tccgcttggc tcgaccagga tgcaagtaac caagtatttt tccttgttcc
      241 caactacagc aaattcgaac aggaacaaga aattttagcg gagatgcgaa gaagaagcgg
      301 aaaaaaaact ttcagcacaa caaacgttca agtatttagt ttttatcgtc tggcttggta
      361 tttgctgcaa cagacaactc ttcttacagg aaatgaatta tcagaatcag gatctgcaat
      421 gatcttgcga cagattttag aaaaaaatga ggaagaatta acaatctttc gcggagaaat
      481 caataaaaga gggtttattc gtcaattaca agaactctac agtgaattgc gtacaggaaa
      541 tattcaacca gaagacctga tgcttttatt tggtgattca cctaatgcag aagaccagca
      601 acttaaaatg aaagacttga agctgatttt ttcagcttat gacagcgaat tgactcaacg
      661 tgcactaaca aatgaagatg cactatctat actagcaaat tatatgcaga cacaagattt
      721 caacaacgtc aaatttattg tcagtggttt ttctcgaatc aatggtcaag agtatcaatt
      781 actgcaggca atgatggaaa aggggcaatt ggttgtcgat ttattgctgg atcgtcctta
      841 tacagcggat cttccagaag tacttacgct ctttcatgag actggaaagc tttatttccg
      901 tctttttcaa agtgcaagag aacggaaagt ccctatatta gtggaccgat ttgcaccaga
      961 gcagactcgt ccgacagagc ttcaactgct gcaaacaatg tgggaagaga catctagcca
     1021 aaagaaagcg gcagtacaca aaataggggg acaagagcat ctccatttgt gggaagctga
     1081 aaatccaact gaagaagtcc gacaattagc tgttgaaatc agacgactag tcagtgaaga
     1141 aaactaccgt tatcgtgata tccaagtgct gacaaaaaat atgtctttat atggaaatat
     1201 ccttcggccg atctttcaag aaatggcaat tcctttttat atcgatgaag aacaaatgat
     1261 ggaaaatcat ccgctgatcg aatggatcaa cagtctgttc gctcttgacc gctattctta
     1321 tcggctaagt gatattatgc gttttttcaa aactgaatta tttctgtcga cggaagcaga
     1381 aaccgaactt gatgaatgga aacaagagcg agatgaattc aggaaaaaac tggacatcac
     1441 tgaaaatgta gcacttgctt acaattatca aggaacttat tggactcgtg aaaaagactg
     1501 gcagtttgtg gcgtatgatt ttgaagcaga acagattgaa gacgtacagt atttggaaga
     1561 acagtcaaat acgattagac gattatttca gaaagctgta ccagtatttt tgaaaaaaat
     1621 aaagaaaagc aaaacaggac aagaggccgc aaatgttttt tatcaatttt tgatccaaag
     1681 tggcgtacag caacaattat tgttttggcg taatcaggaa gtagacagag gaaacttaga
     1741 agcggcaaga aaccatgagc agacatgggg agcgctgatt gatctgttgg atgaatatgt
     1801 agtaatttat ggtgaatcgg cttttgactg gtcactgttc caagaaatca ttacaagtgg
     1861 tctagaaaat ctaagttatg gtaaaatccc taccgcaatc gaccaagtgc gaatcaatcg
     1921 tttggaatta gttcgaccaa atcaagctgc cgtcactttt gcattagggt tgaatgatca
     1981 agtgtttcca gaccgaaaag agacaaaagg attgttgtcc agtgaagaac gagaagtatt
     2041 aaatcaggcc ttgccagatg gacagttttt attcgaccca tcaaaggaaa gtatctcttt
     2101 tgagcctttc caagcctatc ttgtttttct ttccggaaca gatcgattat atcttagtta
     2161 tgcacaaagc tatgacacag atggttcgct gaagatgtct ccttatttgc gtagaatcac
     2221 agaatattta tctgttcccg tcgagagaaa agagcatttg acgatcgaaa gcgaccctaa
     2281 ttgttatgta gggacttaca gaagcggaat caatacagtc aataggattt atcgattagc
     2341 tttagatgaa aaaaaacctg taccaaagca ctggcaaaag ctcaaagaat tagtactgaa
     2401 ttcgtcatgg cgaaattttg ctttacagat ttttgaaagt caggaacatt taaatgttcc
     2461 tgtttcgatg ccagaaaaaa tggcggaaga gctttatgga aaagagattt atacttcaat
     2521 ttcacgtatg gagaattttt ataattgtga atacaaatac ttcatgcaat ttggtttgaa
     2581 attgaaagaa cgaactgttt atggtctaac tccagcagca actggtgatt tttatcatga
     2641 atcattggat cgtttcttca agctactgtt ttcaaaccaa ttatccttag tgaatatgtc
     2701 cgatgcgcag cgtagagact ttactgaaaa agtgctgcag gaagtattcg gagaacttcg
     2761 gtttgagata ttggatagtt ctgcaagaat gaactatatc cgctaccagt taggacagac
     2821 gattcaaaaa gtgacatggg ctttgcaaaa acagagcaaa aatacaggca tgcatcctct
     2881 tcagacagag attctatttg gtcagattgc tggagcaaaa ggtatcacag gtctggaatt
     2941 accgttgagt aatggtggaa aactccatgt tcgagggaaa atcgatcgaa tcgatgtagc
     3001 ttctgaacaa gaagatactt ggctaagcgt cgttgattac aaatctagcg gacgttcatt
     3061 cgatgtcaca gaagcttatt atggcatggc tatgcaatta ttgacgtatc tggacgtagc
     3121 attgatggat gcggtacgtt tgactggaaa ggcggctgtt aaaccagcag gatcatatta
     3181 tttacatgtt cataatcctg tattgacgga gacagatgat gtagagaaga atacattgaa
     3241 aaaatttagt tatgatggcg tttttgtcga tgatcccgct cttttagaag tactagatca
     3301 ttcactggaa gcaaaagaac aatcactgat ctatccagtg aaaaaaaatg cgaaagatga
     3361 ataccaaaaa ggtagtttca gtaaagacaa atttttcacg gaagaagaac tgcaactatt
     3421 catggcgcat aatcgggaaa atatgcggtt agctggagat gcgattactt ccggagaaat
     3481 caaactaaat ccagcttata aagataaaca gcggattgcc tgcgaatttt gtccattcag
     3541 aagcatctgt acgtttgatg tgatgctgaa agaaaataat taccaccgtc tggagaaaat
     3601 agataaggaa gaagcactgc gtagaatatt gaaaaggagt gaagaagagt gaatcaaatt
     3661 cctttaaaac cgcaaaatga acgattcaca gatgatcagt ggcaagcgat ttttgatcaa
     3721 ggagataatc tattagtctc tgcgtctgcc ggatcaggta aaactacagt gcttgttcga
     3781 agagtgattg aaaaactaaa aatgggtttt gatatcgatg aattattgat tgtgacattt
     3841 actgaagctg ccgcaagaga aatgaaagaa agaattcaag aggcattaca agaatccgtc
     3901 aatagcgaaa gtgaccctgt acgtagacag cattttacta agcagttagt tttattgccc
     3961 acagcgaata tctcaacctt acatgctttt tgcctgacag ttattcgacg ttattattac
     4021 ttaattgata ttgatccagt ctttcgcatg ttaacagatg aaacagaaac gatcttgatg
     4081 aaggaggatg tgtgggacga gctgagagaa gcactttatg cagaaaatga tgaacgattt
     4141 ttccaattaa caatgaattt ttctaatgat cgttcagatg acggcttgac gaatctggtg
     4201 ttttctcttt atgagtttgc ccgtgccaac cctgaccccc aaaaatggct agaacagtta
     4261 agcgataatt atcgtttgcc tgaggggcta gcgaaatctc gattatatca aaaacaaatc
     4321 cgtcctttag ttttggcaga tatataccaa tgtgtacagt tatatgaaca gatgactcag
     4381 ttagcacaag gtgaaggatt agaaaaaatg catgagcaag tagctggaga acaacagcag
     4441 atcaagaata tctatgaagc gttctcgcaa gatcggttag aagaagctta tgctggtttg
     4501 gaacaactaa ctttttcaac ttttaagagc agtcggaaag cagaattgaa agaaatttcc
     4561 aatgaagtca agggcatgag ggataaagca aaaaaactga ttcagcagat aagtaaaagt
     4621 tattttcctg tttccccaag ccaaatggaa gaattaacgg acaaagcact accgttagtc
     4681 gaagaaatga caaaagtcac gcaaagcttc atggacggtt tcagtatgcg taaacgggaa
     4741 aaaggtgtgc tggatttcaa tgatctggaa catttagcgt tgcagattct cacagaaaaa
     4801 acgaaagatg tatggctgcc aagtgaagct tcaaaacatt atcgaaaaaa attcaaagaa
     4861 gtaatggtag atgaatatca agacgtgaac caattacaag aagccatttt atactggcta
     4921 agagaacccg atgatacaaa aggaaatatg ttcatggttg gggatgtcaa acaatccatc
     4981 tattctttcc gtttagcaga tcctagttta tttatcggga aatacgagaa tttctcaaag
     5041 aaagaaggcg gacgtcgaat cgttttggct gagaacttcc gctctagaaa agaagtcctt
     5101 tcttttacga atcttatttt tgaacaacta atggatccag cagtgggtca aatcaattat
     5161 gacgaagcag ccaaactgat ccaaggattt tcagactttc cagagaatga acagtttgag
     5221 ccggaaatca tgatctatga aaaagaacag gaagaatcag agattgagat tccgacggat
     5281 gatatactgg aagacaaaac agaaggcgaa ctttttatga caggactaaa aattcgtcaa
     5341 ttaattgatt cctcctttat gatttatgat aaaaaatcaa aaaaaagtcg tccgatagag
     5401 tacaaagaca ttgttctctt gacacctaca aagaagaaca acttaacgat tttagagatt
     5461 ttcaaaacat tggatattcc attagagatg aatgatgctc aaaactattt tcaagcgaca
     5521 gaaattcgca cgatgatctc gctgctacaa ttaatcgata atccctatca agatattccg
     5581 cttgcggctg tattgcgctc accgatcgtg ggtctgattg aaccagaatt agccagcatt
     5641 cgattagcta accgtgcaca tacttactat gatgctgtgc ttgcttatca agcatcaaac
     5701 gaagatgaat tagcagctaa gctggaacat tttggcaagc aactagagca ttggcgagaa
     5761 cttgctcgcc gttcttcgat cactgacttg ttatgggata tctattatga gacgggatat
     5821 ctagagtatg tggtcggtct gccagcaggg gcacagagac aagccaatct ttatgcattg
     5881 gttgatcgtg ccaaagctta cgagcaaagt tctttcagag gattatacca gttcgtgcgt
     5941 ttcattgaaa aaatgcaaga aaaagataaa gacttagcag aaccagtcat ttcaatagaa
     6001 gataatgctg ttcgcgttat gacgatccat gcaagtaaag gcttggaatt cccagttgta
     6061 tttttgttag atatgacaaa ggagttcaac ttacaagact tgcgcaatcg gtatgctttt
     6121 gaagaaaaac ttggagcagg aattcgctat atggatcctg aaacacgagt gttatatgat
     6181 acgttaccgt ttcaagccat caaattggca aagcaaaaca aattgttgtc ggaagaaatg
     6241 cgtaaattgt atgtaggatt gactcgggca gaacaaaagc ttttcattgt cggttcctat
     6301 aaaaataaag agcaaatgat ccaaacgtgg tctgaagcag ctgatcatga ggagttagtt
     6361 tttgatcctg cgctacgttt gaagggcaga agcagcttga tgaattggat tggttacgga
     6421 ttgatccgtc atcctgaaat gcagaaatac ttagaggaag aaatttcgac ttctcttctt
     6481 caacattcaa atgcacagtt ttctatttca tggatgaatc agcaatcgat cattgaacaa
     6541 agacagttgc ttgctgaaaa agaactggtt aacttagatc agcagatgaa agaagatgaa
     6601 actttattag cagattcgtt acaaaaacgt ctggcatatg aatatcctta tcaagcttct
     6661 tcacaaacca caagttatca atctgtatct gaaatcaaac gattattcga agatccagat
     6721 gatacacaag aatcacgttt gactctggaa agcagtcaga acaaagcagc atcccgtcaa
     6781 tttcgttata cgcaagaaca actagcagaa ccaaaatttt tgcaaaaaga tcggcaagtg
     6841 tcagcagctg ctgttggaac tgctacgcat gctttgttac aattactgcc attagagatg
     6901 ccaactacag agagtatcca tcaaaaatta caggaattgg taaggaaacg gttagtagac
     6961 gaaaaagtcg cgaaaaaagt agatgtttct tctattatat ggttctttca aacagaactt
     7021 ggtcaacagc tgattgccaa caaagaaaac gtaaaacgag aacaaccttt ttccatgctg
     7081 cttcctgctg acgaagtatt tcaagactat ccaaatcaag aagatgaact attaatccac
     7141 ggaatcgtcg atggatacct agaagaaaaa gatcatctca atatctatga ttttaaaaca
     7201 gattttattt tacatccgga tgatccagca gaaatagatg caatcgtcca aaaatatcaa
     7261 ggacagctaa gactttacca acaagcaatg tcagaagctt tgaataagcc agtagaaaat
     7321 gttttcctaa ttttgcttcg agtgaaacaa attataaaca ttaacaaata attataattg
     7381 agcaaacttg acttatgttt tgtaaggatt atgctatact ctgtgaagag gtgagtatga
     7441 tggagctaac acagacagaa ttcgttttat gccctaaatt tgaaaaaaca ttctctattt
     7501 taggaaaaaa atggaacgga ttgattatcg atgtactact cgaaaacggt ccgcaacgct
     7561 ttgttgagct tgcggcaaaa atcccgatgg tcagtgatcg agtattagtc gaacgcttga
     7621 aggaattaga aaaagaagga atagtcactc gaaactttgc ctgtagcgaa aagagccgtt
     7681 cagaatatct cttgactgaa aaaggagcag acttgcaaaa agcgatgcgt caaatacaag
     7741 tttggagcga aaaatggatc acagaggaag aatgcggttg attttgtaaa aaaaatcttg
     7801 taaactatag tccatacgaa taagtaaaga acgatgacga aaaagagtag tttggctaaa
     7861 ccagttcagg gagatctgtc gcagactgaa agcagatcct gtgtttgaaa aatgaagttc
     7921 actttccgag ttcgtctgat aaaatcagac cggcaattgt cgttaacgaa gtaagagtgc
     7981 ggtgtaagtc atttatccgc aaattaggat ggtaacgcga agacctcgtt ccttaacagg
     8041 aatgaggtct ttttgttttg tatttggaat ttaaaggagg aacaagcaca tgtctctaca
     8101 agcacaatta gagagcttga aagaagcaac gctcgtcaaa atcacagcgg cacaagattt
     8161 aaatgaatta aatcaaatac gtgttgaaac gttaggaaaa aaaggaccga tcacagaagt
     8221 attacgtggg atgaaagatc taagtccaga agagcgacca aaagtcggaa gtttcgcaaa
     8281 tgagatccga gatcttttga cagaagagat cgagcgaaaa aaacaagtat tagaagaaga
     8341 agcgttaaat gcagctttgg caaaagaaac aatagacgtg acactacctg gtagaaaagt
     8401 aaaacatggt acacgtcata tcgtgacaca aatcatggaa gaaattgaag atatttttgt
     8461 gggtatgggc tatcagatcg tagaaggata tgaagtagaa tctgatcatt acaatttcga
     8521 acggatgaat ctgccaaaag atcatccagc aagggacatg caagatactt tctatatttc
     8581 agacgagata ttgattcgga cacatacttc accagtccaa gcacggacaa tggaaaaaca
     8641 tgatttctct aagggtgctc tacgaatgat ctcgccaggg aaagtcttcc gcagagatac
     8701 agatgatgcg acccacagtc atcagttcca tcaaattgaa gggctagttg ttgataaaaa
     8761 catcacaatg ggcgacctta aaggaacatt agaagtcgta atgaaaaaaa tgtttgggga
     8821 agatcgtgaa attcgtttgc gtccaagtta tttcccattt acggaaccat cagtagaagt
     8881 agatgtcagc tgtttcaaat gtggtggtgc cggttgtaac gtatgtaaat acactggatg
     8941 gatcgaaatt ttaggagctg gcatggtaca cccgaatgtg ttgaagatgt caggaatcga
     9001 tccagaagaa tattcaggct ttgctttcgg ccttggacca gaccgcgtag ctatgttgcg
     9061 gtatggagtg aatgatatcc gtaatttcta tcaaaatgac ttacgtttct taagtcagtt
     9121 caagggggag taagaaaatg ttagtttcat ataaatggct caatcgttat gtagatttat
     9181 cgaatgtaac accaaaagaa ttagcggata agatgtctgt gacaggaatc gaagtagagg
     9241 gagtaacggt tccagaagaa ggcttgaaaa aaatcgttgt cggtgaagtc aaggaatgcg
     9301 taccacatcc agattctgat cacctttcta tttgtcaagt cgatattggc gaagacgaac
     9361 tgtctcaaat cgtttgtgga gcgcctaatg tgaaggttgg aatcaaagtg attgttgcac
     9421 ttcctaactc acgtatcgct ggtaatgtaa aaatcaaaaa aggaaaaatg cgtggccaag
     9481 tatcaaatgg catgatttgc tcattacaag aaattggcta ttcagatagt gttataccga
     9541 aagaatacgc tgaaggtatc tattatatgc cacaagaagc tgtaaatggc gatccagttt
     9601 ttccttattt ggatatggat gattcgatta ttgaattatc tatcacaccg aatcgtgcgg
     9661 atgcattgtc aatgcgtggc gtagcttatg aagttggggc aatttatcgt cagactccta
     9721 cattcgatga cgaaaagctg gtagaatcct cacgtcctgc tagcgataaa attcaagtgg
     9781 aagttgaaga taaagaagca gttcctgctt atcaaatcag aatcattgaa ggagtaagaa
     9841 tccagccaag tcctcaatgg ctgcaaaatc ttttgatgaa cgaaggcatt cgtccaatca
     9901 acaatgtagt agatgtgacg aactatattt tgcttttatt cggacaacca ctacatgcat
     9961 ttgactacga caaattgaaa tcaaaagaga tctttgtccg ccgtgcaaaa gaaaatgaaa
    10021 cattagtaac actagatgga gaatctcggg aattgacaac tgacaatctc gtcattacta
    10081 acggcaaaga accagttgct ttagctggtg tcatgggagg gttagactca gagatcacga
    10141 atgaaacaac aacagttgca ctagaatctg ctttatttga tccaatttcg attcgtcgta
    10201 cagcaaaaca atttaatttg cgtagtgaat cctctgcacg ttttgaaaaa ggaatcaacc
    10261 atgcgactgt tggagaagcc agtgaggttg ctgcagcaat gatcgtttct cttgcgggtg
    10321 gggaagttct acaaggcgcg gtcaaaggct cagagatcaa agcagaagac gtatcagtga
    10381 agatttcact tgaacgcatc aatcaatatt taggaacatc tcttgtcgtt aaagaagtaa
    10441 atgagatttt tgaagcactt ggatttagct atgaagaaaa ggagtatgtt tattccgtag
    10501 tagtaccacc aagacgctgg gatatccaaa tcgaagcaga tattatcgaa gaagttgctc
    10561 gtatttatgg atacgaccag ttgccatcta cacttccaag tggagaaacg gttgctggaa
    10621 gcttgtcaaa agaacaagaa actactcgta aaattcgtac aatctttgaa ggaagcggat
    10681 tgagcgaagc aatcagttat gcattgacaa cagaagaaaa atcaagacag tttgttataa
    10741 atgaaagtaa tattacacgt ttggactggc caatgagcga agaacgttct gtattacgaa
    10801 tgaatttgat cagcggacta ttagatgata tcagttataa cactgcccga aaaaatacag
    10861 agattgcttt atatgaaatt ggtcgggtgt tcttccaaga aaatgatcca cgtacacatc
    10921 tgccgcaaga agtaaaacat gcagcaatcg ctatttctgg tatttgggaa gaaaaagatt
    10981 ggcagacgaa agctcaaaat gtagactttt tcaccatcaa gggtctttta gaaaacttat
    11041 ttgaacaact aggcatcact gaagatgtcc attatcaaac agtgacagaa atgaaggaaa
    11101 tgcatcctgg aagaactgct gcgatttacc ttggcgaaaa atttattggt tttgtcggtc
    11161 aagtgcatcc aacgattaca aaaagttatg atattccaga aacatatgtt gctgagtttg
    11221 atttatctgc tatagtggaa gctgctcaaa aagggatcgt tttcgaagca gtgacaaaat
    11281 tcccagcagt cagccgggac atcgctttgc ttgtaaaaga aactgtgaca aatcaggaat
    11341 taacagaagt tatccgttct gctgccggaa gatttttaac agatatccaa ttatttgatg
    11401 tctatcaagg agaaaacatt gaaaaaggac ataaatccat ggcgtatagc ctgacattcg
    11461 tcaacccaga agcaacattg acagatgaag aaatcaataa agcaatggaa aaagtcgaaa
    11521 aagcgttggt agaaaaccta gaggcaagta ttcgctaaga aggaaaatat agtaagtaac
    11581 gatcaagtag cttttgaaga ccatacattt gaaaaaaaga tgtgacacaa cttcgtcaca
    11641 tctctttttt tgattttaaa aaaggaatag cacagagacg agataaaaaa acaggccttt
    11701 acagcctgct tttttagagt aaatgaatta atggaagtaa ttgattccca ttgctgattt
    11761 tacttcagaa agtgtttgtg cagctacttt agcagcttct tcactacctt tacgtaagat
    11821 ttccataaca gctgaaggat ctttttccaa ttcttctcgg cgcgcacgga tcggtgcaaa
    11881 ttcagcttcc aatacatcaa tgaggtatcg tttgatcttt acatcaccaa gtccgccgcg
    11941 gcggtaatga gctttcattt cttcaattgc ttctttatct gttccgaata catccaagta
    12001 ggtgaagacc atattccctt ctacttgtcc aggatcttgt acatggatgt ggtttggatc
    12061 agtatacata ctcatgactt ttttctgtaa gacatcggca ggatcggaga tataaatacc
    12121 gtttcctaac gatttactca ttttgccatt tccatcgatt ccaggtaatc tacccattcc
    12181 ttttggtggg aatacgcctt ttggttccac caagacttca ccatatgtat gattgaagct
    12241 ttgtacgatt tcctgtgtct gctctagcat tggtttttga tcctcaccaa caggtacaag
    12301 attcgctttg aatgcagtga tatctgctgc ttgagaaaca ggatagataa agaatcctgt
    12361 aggcacgctt tcaccgaatt ttttttgttc gatttctgtt ttgacagtcg gattgcgccg
    12421 aacgcgtcct acgctgacta agttcaaata atacatggtt aattcagcta attccggaat
    12481 ttgtgattgg ataaataaag tagattttgc aggatctaat cccacggcta aatagtctaa
    12541 agcaacttgc aagacatttg atgaaacctt ctcaggattt ttagcattat ctgtcagtgc
    12601 ttgcatgtca gcaatcatga caaacagttg gttgtcttca tctgcctgca tagccactcg
    12661 ggtttttaga gaaccgacgt aatgccctaa atgcagcttc ccagttggtc gatctcccgt
    12721 taaaataata tttttcaaaa aagacacctc tttacgattt gtaagtctga gcacttaata
    12781 tcttaccgtt tttcctgaaa agaaacaaca gttcctaagg aaaaaacaag gaaagaggat
    12841 aaaataacta atagattaga aatggaagaa atatttatgg gagaataaac gttctaagaa
    12901 actttacaaa ttactaaact atcagatata atgtatttta ttatacatct ttaaaagaaa
    12961 tcttactaaa taataatggg agtgtaattc tatggcaatg attaaatttg atcacgtgga
    13021 taaatattat ggcaagttcc acgcattaaa aaatatcaat ctcgaattcg agaaaggaga
    13081 agttgtcgtt gtcattggtc cttctggttc aggaaagagc acgatgcttc gctgtatcaa
    13141 tggattagag acgatctctt caggtaagct gctgattaat gatactgact tgcatgataa
    13201 aaagacaaaa ctgacagaag tccgaaaaaa catcggcatg gttttccaac atttcaattt
    13261 gtacccaaat aaaactgttc tggaaaacat caaattagca ccaatcaaag tgttgaagca
    13321 agaccaggca actgcagtca aaaacgcgga gaaatttttg aaaactgtca atatgttaga
    13381 taagaaagat tcttatcctt ccatgttatc cgggggacaa cagcaacggg tggcaatcgc
    13441 cagaggttta gcgatgaatc ctgagatgct gctgtttgat gaacctactt ctgctttgga
    13501 tccggaaatg attggtgatg tattagacgt tatgaaaaaa ttggctcatg acggtatgtc
    13561 aatgattgtc gtgacacatg aaatgggctt tgccaaagaa gtggcggatc gtgttatctt
    13621 catggctgat ggagaagtat tggaagacag cagaaatgtg agagactttt tcgaaaatcc
    13681 aaatgaagtc cgagcacagc aatttatcag taaagttatc aatcattgat aggaggaaaa
    13741 agatgcgcag gttaaaaaaa atacgcctga ttttcctttt aggcattgct tttcttgcac
    13801 tagccggatg taaaggaaat gggttggcaa atgaagatat tttagagcga agcaaatcta
    13861 ccaatgaaat catctggggt gtcaagtatg atactcgatt atttggaatg atggatattg
    13921 agagcagaac tgtacaagga tttgatgtag atattgcaaa agcaatcact aaaaagatcc
    13981 ttggtgacaa tggaaaaaca gaatttgttg aagtcacttc taaaactcgg attccattat
    14041 tgaaaaacgg aaatatcgat gcaattattg caacgatgac gatcacagat gaacgaaaaa
    14101 aacaggttga cttttcagat gtctatttcg atgctggcca agctttgttg gtaaagaaag
    14161 gaagccaaat caaaagtgta gatgatttga atgcttctac aacggttcta gcagtcaaag
    14221 gttctacatc ggctgcgaat atccgacagc atgcaccaga tgcaaaaatt ttagaattag
    14281 aaaactatgc agaagcattt actgctttac aatctggtca gggagacgcg atgactacgg
    14341 ataatgcgat tttgttaggg attgcagatg aaaatccaga gtatgagtta gttggtggaa
    14401 cgtttactaa tgagccttac gggattgcga tcaacaaagg gcaagaaaac ttcttgaaag
    14461 ctgttaacca agcgctagag gaaatgcatg cagatggcac atacgataaa atctatcaaa
    14521 aatggtttcc aaatgaaact gaaggaaaag tagagtaagg gggagaggca atgagcgaat
    14581 tgatacaaac ttatggacca atctttgtcg acgggttcaa gatcacattg ttttcgagtg
    14641 tcttagcact tgtgtttagt ttgattatcg gaacgttaat ggcaatcttc caattatcaa
    14701 agaataaagt agtgagcgcc ttagcaaagg catatgtaga attttttaga aacattccat
    14761 tattaatcat cgtgatgttt ttttatgttg tcgtaccgat ttattggttt aatctagatg
    14821 gattccaagc aggtacgatt ggattaacga tctatacatc tgcttttatt gctgaaacag
    14881 tacgttcagg gattcaaacc gtaccaaaag gtcaaacaga agcaggacta tcttctggtt
    14941 ttacctattc agaaatcatg cgttatattg ttttaccgca agcattcaaa atcgttatcc
    15001 cgccattagg caaccaattt atcaacctcg taaaaaactc atctatttta gcaatcgtag
    15061 ctggtttaga tttgatgtat caaggagact tgatcgctag tgaaacattc aataccttcg
    15121 atacgtatat catcgttggg ttattttact tagttatcac gctaccgtta tcttacttaa
    15181 tggtttatct tgagaaaaaa tgggcggttc gttcatagaa ggaggggaaa tacatggatt
    15241 ttagcggcgc tttttcatgg atgaatatcc gttttttatt ggaaggatta aaggttactg
    15301 ttgaagtttc attgttttcg atcattttta gtttcttgat cggtggtctg acaggtgtga
    15361 ttcgttttgc gaatatccct ttcttttcca aaatccttgg tgtagtgatt gatattatac
    15421 ggaatcttcc attattattg atcattttct tcacttactt tgccttacct caaatcggca
    15481 ttcagatgaa catcttctgg tccgctgtcg cagcattaac gatttttgag tcagctatgt
    15541 tgtcggaaat tttccgagct ggactcaatg ctgttccaaa aggacagatg gaagcaggat
    15601 tatcaacggg actgacttat gtagaatcga tgcgaacgat cgtgttaccg caagcattta
    15661 aatcaatgat cccggcaatt gtgagccaat tgatttcact gatcaaagat acttcattag
    15721 cagtaatcat ttctttacct gaactttctc atcaggcacg tattatttat ggacagaaca
    15781 ccaattatgt gcttccgatg ttcatcatca tgacacttat gtacttcgtg gtatgttacg
    15841 cgctttctct gctttccggt tacttggaaa agagacggta cagttattaa tagatgattc
    15901 ttttagcggt tgtggcaaac taaagtcaca accgcttttc ctctttccat tcgaaaaaaa
    15961 gagatttcga ttgttttaag caagtaagag cacttgaaaa ttcacgctaa aaagtacaca
    16021 atagggagga tagaatttat tgaaaaaaag gatgatacga ttgacagata atcgccctat
    16081 cggatttatt gattcaggtg tcggcggctt gactgtagta aaagaagccc tgaaacaatt
    16141 accgaatgaa aatattttat ttgtaggaga cacagcacgc tgcccatatg gccctagacc
    16201 cgcggaacag gtaatccagt atacttggga aatgacggat tatctggtgg agcaaggaat
    16261 caagatgctg gtgatcgcct gcaataccgc aactgcggtg gctttagaag aaatcaaagc
    16321 tgctctttct attccagtca tcggtgtgat ccttcccggt actagagcgg cagtaaaaaa
    16381 aacacaaaat aaacaagttg gcattatcgg tacgattggt acggtaaaaa gtcaagctta
    16441 tgaaaaagca ctgaaagaga aagtaccaga attgactgtg acaagtcttg cttgtcccaa
    16501 atttgtttca gttgtcgaaa gtaatgaata ccattcatcg gtggcgaaaa aaattgtggc
    16561 agaaacatta gctcctttaa ccactaaaaa aatcgataca ttgattttgg gatgcaccca
    16621 ttatccatta ttacgcccca tcattcaaaa tgtaatggga gaaaatgttc aactgatcga
    16681 ttctggagca gaaacagtag gtgaagtatc tatgctgtta gattatttca atttgagcaa
    16741 ttcaccgcaa aatggtcgga cattatgcca gttttataca actggctctg ccaaactttt
    16801 cgaggaaata gctgaagact ggcttggaat cggacactta aatgtagaac atatcgaatt
    16861 gggaggaaaa taaggatgcg tcatgacggg agaaaagccc aagagcttcg caaactggag
    16921 cttcaaacaa atgttttcaa atatccagaa ggatcggtag tgatccgttt tggtgataca
    16981 acagttattt gttctgcaac aatagaaaat tctgttcccc cttttttgag agaaacagga
    17041 acaggatggg tgacagcaga atatagtatg ctgcctcgag caacgagtac gagaaatcgt
    17101 agagaaagca gcaaagggaa actttctggt agaacaatgg aaatccagcg tcttattggt
    17161 cgttccttgc gtgcagtagt agacttggaa aaattaggag aacgtagtat catcgtagac
    17221 tgcgatgtga tccaagcgga tggcggtact agaacagcaa gtatcactgg ggcatttgtg
    17281 gcattgaaat tagccattga aaaactttta agagaaaaag aattgtcaga agatccaata
    17341 aaagaacatc ttgctgcagt tagcgtagga atcttaccag atgggacttg cgtcacagat
    17401 ttggattacc aagaagattc cgcagcactt gtagacatga atcttgtaat gacagaatct
    17461 ggaaaattcg tggagattca aggaactgga gaagaagcaa cgtttgatgg tgaacagttg
    17521 aatgaaatgc tcttttttgg aaaaaacgcg attgaagatt tgatcaaaga gcaaaaacat
    17581 gctttgctaa cagagtttgc tcaaaatgat gagcggatag aagaaacaaa aacaatcatc
    17641 attgcgacaa gaaatccagg aaaagcagaa gagtttcgaa atatgttcaa agaagcggga
    17701 tatcatgtaa aaacattact tgactatcct gaattgccag atgtggaaga aactggcagc
    17761 acatttgaag aaaatgcccg attaaaagca gaaacgattg cacaattgct tgatcagcca
    17821 gtcttagcag atgattcagg tttgaaagtg gatgctttag gcggtatgcc aggaatctat
    17881 tctgcacgtt ttgcagggga acaaaaaagt gatgcgggga ataacgccaa attattatat
    17941 gaattgacag atgtgccgga tgaaaaaaga acagcacaat tccattgtac gttagtattc
    18001 gcggcaccca aaaaagacag ccttgtagta gaagcggaat ggcccggtcg agtagcccgt
    18061 ataccatctg gcgaaaatgg ctttgggtat gatcctttat ttatcccaga aggtaagaaa
    18121 caaacagcag cagaattatc gagcgaagaa aaaaacaaga tcagtcatcg ggctcaagca
    18181 atgaaaaaat tgagtgccga gtggaaacaa tggctggaag gagagagata gatgcggtat
    18241 ttagtagtaa gtgataatca tggagacaga gatatcctcg ttgacttagt agatcgttat
    18301 cgttctgaag tggatatctt tttccattgt ggtgattcgg aattagaagc ttctgaccca
    18361 ttatgggata cattccaagt ggttcaaggt aattgtgatt atggaccagg attcgaacag
    18421 aaaaaagtga tccaaactgg tcaggataca gtattcatga ctcacggtca tttatcgaat
    18481 gtccgatttg gtttgacaca gttagcaatc gaagctcgag ctgcaactgc cgatatggtg
    18541 ttttttggtc atacccatca aataggctgt gagatggatc agaaaatcct gtttttgaat
    18601 cctggtagca tttctcagcc tagaggcccg atccaaatcc catcatatgc tcttatcgaa
    18661 agtttagatg accaaatttc tgttcaatac tataaccgtg cccatcaggc agttgaagag
    18721 ttggcctttt ctttttcgag agagcgctga ataaaaaaac tctaattttt aataaaaata
    18781 agctttattt tatgaatttt gcatccggtt tcggtaaaat agtagtgttg aatgaaatag
    18841 aaatggaggc catcagatga ttggacctat agtaagagaa atgctgctgg aaaatcagga
    18901 aacgtttttg attcctgcag aaaatgtggc aaatgttatg tatcatcatc cattgtccca
    18961 tggtttactt gtgttatcga aggtcggtta ttccaaaatt cctgtattag gaaaagatga
    19021 tcactttgta ggactagtaa gtttatcaga tgtggtcaat aaaatgattg acttgcaaac
    19081 aatcagcatg gacccgttag aaggactaac ggttgctgat gtgacagaaa cagatgttcc
    19141 gacaattgat gaaaattggg aactagaaga agtactccat ttgttagtcg attcgccttt
    19201 tttaccggtt gtcacagaag atcggatttt caaaggaatc attacaagaa aagagctgct
    19261 taaagccgta aacttcatgg tgcatgaatt agaacgtcgt aatatattgt ctccgaaaat
    19321 cgaagaatta aaagaacaaa tcgatatcgt gagctaaaga acgagaaact aaaagataat
    19381 caaaaaataa gtagaaaaaa cagcgtacca gttttgactt tatcttgaaa aagaggctgg
    19441 gaca
//
LOCUS       DAXWLG010000039        19393 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXWLG010000039 DAXWLG010000000
VERSION     DAXWLG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.039"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(40..636)
                     /locus_tag="ACGE4T_001101"
     CDS             complement(40..636)
                     /locus_tag="ACGE4T_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8386396.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            986..1330
                     /locus_tag="ACGE4T_001102"
     CDS             986..1330
                     /locus_tag="ACGE4T_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFF8386397.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1331..1750
                     /locus_tag="ACGE4T_001103"
     CDS             1331..1750
                     /locus_tag="ACGE4T_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFF8386398.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1842..2192)
                     /locus_tag="ACGE4T_001104"
     CDS             complement(1842..2192)
                     /locus_tag="ACGE4T_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386399.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2158..3489)
                     /locus_tag="ACGE4T_001105"
     CDS             complement(2158..3489)
                     /locus_tag="ACGE4T_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFF8386400.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3744..4310
                     /locus_tag="ACGE4T_001106"
     CDS             3744..4310
                     /locus_tag="ACGE4T_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8386401.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4455..4542
                     /locus_tag="ACGE4T_001107"
     tRNA            4455..4542
                     /locus_tag="ACGE4T_001107"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4487..4489,aa:Ser,seq:cga)
     gene            4878..5852
                     /gene="bsh"
                     /locus_tag="ACGE4T_001108"
     CDS             4878..5852
                     /gene="bsh"
                     /locus_tag="ACGE4T_001108"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFF8386402.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6177..8072
                     /gene="asnB"
                     /locus_tag="ACGE4T_001109"
     CDS             6177..8072
                     /gene="asnB"
                     /locus_tag="ACGE4T_001109"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFF8386403.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8267..8713)
                     /locus_tag="ACGE4T_001110"
     CDS             complement(8267..8713)
                     /locus_tag="ACGE4T_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFF8386404.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8941..9090
                     /gene="rpmG"
                     /locus_tag="ACGE4T_001111"
     CDS             8941..9090
                     /gene="rpmG"
                     /locus_tag="ACGE4T_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8386405.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9178..9696
                     /locus_tag="ACGE4T_001112"
     CDS             9178..9696
                     /locus_tag="ACGE4T_001112"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8386406.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9773..10729)
                     /locus_tag="ACGE4T_001113"
     CDS             complement(9773..10729)
                     /locus_tag="ACGE4T_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8386407.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10895..11701
                     /locus_tag="ACGE4T_001114"
     CDS             10895..11701
                     /locus_tag="ACGE4T_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386408.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11698..12687
                     /locus_tag="ACGE4T_001115"
     CDS             11698..12687
                     /locus_tag="ACGE4T_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8386409.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12684..13688
                     /locus_tag="ACGE4T_001116"
     CDS             12684..13688
                     /locus_tag="ACGE4T_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8386410.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13820..14362
                     /locus_tag="ACGE4T_001117"
     CDS             13820..14362
                     /locus_tag="ACGE4T_001117"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFF8386411.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14382..15080
                     /locus_tag="ACGE4T_001118"
     CDS             14382..15080
                     /locus_tag="ACGE4T_001118"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8386412.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15101..15313
                     /locus_tag="ACGE4T_001119"
     CDS             15101..15313
                     /locus_tag="ACGE4T_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFF8386413.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15313..16275
                     /locus_tag="ACGE4T_001120"
     CDS             15313..16275
                     /locus_tag="ACGE4T_001120"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFF8386414.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16293..16688
                     /locus_tag="ACGE4T_001121"
     CDS             16293..16688
                     /locus_tag="ACGE4T_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8386415.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16851..17216
                     /locus_tag="ACGE4T_001122"
     CDS             16851..17216
                     /locus_tag="ACGE4T_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFF8386416.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17213..19024
                     /locus_tag="ACGE4T_001123"
     CDS             17213..19024
                     /locus_tag="ACGE4T_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFF8386417.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19086..19253)
                     /locus_tag="ACGE4T_001124"
     CDS             complement(19086..19253)
                     /locus_tag="ACGE4T_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFF8386418.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6276 a         3299 c         3944 g         5874 t
ORIGIN      
        1 taacattgtc aatcaatgac taattttaca aaataaatat tatccaagaa gaccacttag
       61 atattgacct ccataaatca aggccatact atataagaaa atcgacagtg gtttagcaat
      121 caaaatgatt agtgtatatc gtttcactga catgtttgtc aatccagcca tcaaacataa
      181 cgcatcgtca ggagcaactg gaagaaaaat agctagagca aacagcttct caaaacggtt
      241 ttgattatcc agccagccaa tatatttgtt atatgtctta tcgctaatca tggaaagtat
      301 gaatggcttt ccataacgac gccctagcag aaaaatgata atcgaaccaa tgcagattcc
      361 aatatagtta tagataaatc cagcataagg gccaaatatc aggacaccag ctgcagtact
      421 gatccctcca ggaatgattg gaataactac ttgcaggatt tggatgaaga caaaaataat
      481 cggtcctaaa atgatggaat cacctactaa tcctcttaac gcattcaaat ctttgaaaac
      541 acctaaatta ataaaataaa tcgtcagcgc gattgatagg cctaatccga taatggatat
      601 gaaatttatc agttttcgag aggcagcaat attcattatt catcaactcc ttagaggttc
      661 tttgttaact atactttact agaaaagcaa ctaactgcgc cattttttga tatgacagaa
      721 aacaaacaat tttgtaagtt catcataagt ataaaagatt gatctcaaaa aaacatccaa
      781 ctaaagactt atttaataac ttgttttttt taaaataaaa aaagcgttag caactatgct
      841 taaaaagcat atcgactgaa taaactaagc attggacagg ctgaaagact acatttaaaa
      901 tgaaatgcga agaaacaata gaaaataaag ttgccttaga accatagttg aatcacctaa
      961 cagtcgtgaa gggagaataa gagaaatggc agaaaaacaa accgcaggac gagatcaatt
     1021 aggagatttt gcaccaaaat ttgcagaatt aaatgatgat gttttatttg gacaagtatg
     1081 gtccagagaa gaattatctg ctcatcagcg tagtatcatc accatatctg cattaatttc
     1141 aggaggaaat tttgaacaat tgccagctca tctgaagatt ggcaagcaaa atggcattac
     1201 tagtgaagaa atagcagaaa tcattaccca tttagctttt tatgtagaat ggccaaaagc
     1261 ttggtcagct tttagtttag caaaagaaat ctatttagaa gaaaatagcg aagataagaa
     1321 agaggcgtaa atgatgacaa aacacgaaga aatacacaat ggcgtgattt ttccagcagg
     1381 tgaaaaaaac gaagcttacg ctcaagtatt taccgggcaa agctatctga atatgttagt
     1441 gaatgatccc gaagtttctg ttggtgtagg taatgtaact tttgaaccag gttgtcgcaa
     1501 tcactggcat gtccatacag acggctatca aatcttactt gtgaccggag gagaaggctg
     1561 gtatcaggaa gaaggaaagc cagcaagacg attaatggct ggagatgtcg tcgtaacgca
     1621 caaagggatt aaacattggc atggtgcgac aaaagacagt tggtttgaac atttggcgat
     1681 cactgctggt aattcagagt ttttagaacc tgtaacagat gaagtgtatg aaggattgga
     1741 aaatcaataa tgtcaagctt gaacagctcc tgaaagtaga ttttaatcga actttcagga
     1801 gctgcttttt tgattcagtc ttttataaat agaaaatagg gttaataata ggaatgagaa
     1861 gcacgtcgaa agatcttaat attgataatc ggctgggcat acatgatttc cacgtcatca
     1921 atcaaactac ctaacagatg ataatcttcc cgatcatgtc ggggattacc taatagttct
     1981 tgatagtaga tttccatact agggtcccga ggattgttca gcaaatcagc aagtacatct
     2041 aaatcttctg gctgagtttc tgccgtaccc tgtatctcta tgaaaagccc ttcctcagca
     2101 aattctactc caatacgtat ttcagttatt tcatgggaat ataaataagt aagaagctca
     2161 tcgatgattc tttttgataa ttctttttcc aagtgtcttt ttttcctttc tgacagcggt
     2221 aaagttatct agtatcggta caaggatacc tgcaacgaaa ccagttgaaa aaccactgtt
     2281 atatagattt agtcctccgt gcaaagtgct gacgttatgg acaagagcaa tatgtagcac
     2341 accagctatt acaccgtaaa aactgccgta aaaaccgctt atgggcgcaa gacctgtccc
     2401 aaacacagct gcaacaagca tgccagtact ggtgatctca tgaaaagttc caaacaaaga
     2461 agcaataaat attccgacta gtatcggaaa actgtttttt aaatgacaac caaaagcact
     2521 gaaacccacc gcagataaaa tagcaccgat gacaggtcca tttaactgac cgccaaccag
     2581 caaaacataa gaagacagca tcagaccaac taagcccatg ttgatcaaag tagcaccaat
     2641 accagaatta gcaatgaaat cagttgtcaa ttttccagat gaatcgaaaa tttctcgaat
     2701 ccccgaaagc cgaaaggaat tataatagaa accaattccg actgtcagcg aaaaaaagcc
     2761 aaaaataatc caaatgagag gaaagtgata ttcattgaaa accaatgtat tttcgacgat
     2821 tgaatagctg aataacctca aaacagcagt aaaagtcatc gcaatcaacc cagaggtgaa
     2881 tccaatatta taaagtgtga atccacgatg gaaaactaaa gtttgagcag ctaagggcgg
     2941 aagaagtata ccaattgcta tgccagctaa attccccaat aaaataccta tcagtaaagg
     3001 aaaacgcata ccaaaagtga tataactaac gaccggtgat aatacgctac caaacagtcc
     3061 gatcatgatg tactgagaaa aaggtttatt gacaaataca gcgtatagat acacacctaa
     3121 aatgattgag atagaattat aaaggttttt gccaaaaaaa gagaaaccgg taactgttag
     3181 aatcgaagca aataagggac cgtttaaaat caccttgtgc cgataagcta aaagcacaga
     3241 aagtaaggtc aaaatcccta catttatgaa tgcgcttccg aaaacaccta aagcaaaata
     3301 atctgtcaaa agattgcttg gcgaggtaag aatagttagc aatccactcc aattatcagt
     3361 aggagtatta gaaagcaagc caataaaaag aaaaaaaaca cctgtaaaac caagataaaa
     3421 atactttctt ttctgtgaaa cgattcgttt ttgaggatga atataaataa cgtcagtttc
     3481 gttttccatt acacaccttc tttttcattt tctttaacta taacttgaaa tgaaagattt
     3541 ggacaactat catttaggtc aaaactaaaa atgtaaggga acccattgaa tttaaccaaa
     3601 tttttttaat tttgcaaaaa aaagttaaag aagactgaaa aaaatttaaa atataacgtt
     3661 ttgagtaaaa tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa catatactgc
     3721 agtaaagaag tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa
     3781 aagctgctta tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg
     3841 ctaaaaagat ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt
     3901 taaaaaatac gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg
     3961 aacgtactgg tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc
     4021 atcatagtct ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg
     4081 atttttcata caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat
     4141 cggatgaata tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt
     4201 cattgatgtc atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac
     4261 ggacaattga ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca
     4321 gtatgaattt gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa
     4381 aaagcgattt ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta
     4441 aaaatagtac ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa
     4501 ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa cgattagagg
     4561 ttgtgaagaa gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat agcttttcct
     4621 attttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg
     4681 gaataagggg ctgtgacaag acttttgtca cagcccctta ttgttgtact tttaacctac
     4741 aatatttttt gattccccct gagtactatt ttttataaat gctagtaaat caatcttctt
     4801 tggccttttt tttgataaaa aaaaatacta gatgtaagat aaaagtgtaa aaacgatttg
     4861 ttttaggagg gaaaacgatg tgtaccgcaa tcacttatgc aacgaaagac cattattttg
     4921 gaaggaattt tgattatgag atgtcttata atgaagtagt cactattact ccaagaaatt
     4981 atcgtttcga ttttagaaag gtaaaaaatt tagataaaca ttatgcaatg ataggaatag
     5041 cagctggggt atccaactat ccactctatt atgaggctac aaatgaaaaa gggttgagta
     5101 tggcaggact gaattttcct ggcaatgctg attataaaga attacaagaa ggaaaagata
     5161 acgttgcacc atttgagttt attccatgga tactagggca atgctcaacc atagacgaag
     5221 ctaaagaatt attggctact atcaatctag tcaatatcga ttttagtgaa aaactaccct
     5281 tgtcaccttt acactggtta ttagctgata aagaaaaatc gatagtaatt gaaagtatga
     5341 aagatggact tcatttatat gataatcctg tcggtgtgtt gacaaataat ccgccatttg
     5401 attatcaatt atttaatctg aacaattatc gctcgctatc aaatggaaca ccagaaaatc
     5461 atttttcaaa ccagatcagc ttgaatgttt atagtcgtgg gatgggagga ttgggcttac
     5521 ctggagatct ctcttctgtt tctcgatttg taaaagccac atttactaaa atgaatgctg
     5581 catcaggtga ttcagaatct gaaagtatca gtcaattttt tcatatacta ggctcagtcg
     5641 agcaacaaaa aggagtatgc gatactggag aaggaaaata cgaatatacg atttattctt
     5701 cttgctgtaa cgttgataaa gggatttatt attatcgtac gtatgaagac agtcaaatta
     5761 ctgctgtaaa tataaataaa gaagacttgg atcgtcatga attggtcagt tatccagtca
     5821 taaaagagca acagataaac tatgtcaatt aattaatgaa tagatccgtt tggtatcata
     5881 ttttctgata cagtcataat tagacacgat ctttaggtcg tgtctttttt tgctgttttt
     5941 tttggaaata gcatccacta atattttttt aacacgaaga gaataaggta gcctcaaaaa
     6001 agaaaacatg gtaaattaga tattgggact ttattttctg taaaaacaga aactttcatc
     6061 ttttgttttc acgtgacgaa attgtaagag agaaaatatt cgtccgctat ttattctatg
     6121 ataagatgca tatgaatgaa ataaacagta attttatatt aggatgtgaa agttaaatgt
     6181 gcggtattgt aggttttgtt aacgacaagg acaacaaaaa aacaattatt aatacaatga
     6241 tggatcgaat cgttcaccgt ggacctaata gttcaggaga atatatcgat aaacatgttg
     6301 ctttaggatt cagaagatta agtattattg acttagaggg cggtactcag cctatttaca
     6361 atgaagatcg gacaaagatc attattttta atggcgaaat ctataattac cagcctttga
     6421 gagaagaatt gattgcagct ggccacgttt ttcaaacaca tgcagataca gaagtccttt
     6481 tgcatggtta cgaagaatgg ggaacagaat tattgcaaaa gatccgcgga atgtttgctt
     6541 ttgctatttg ggataatgaa aagaatgagt tattcggtgc aagagaccac tttgggatca
     6601 aaccatatta ttatgcagaa atgaacggaa catttatgtt tggttcagaa atcaaaagtt
     6661 ttctgcctca tcctgatttt aacaaagaat tgaacaaaga agcgttgaaa ccttatatga
     6721 ctttccaata ttctccatta aacggcgaaa cattcttcaa agatgtctat cgtttgcctg
     6781 aaggccatta ttatacgtat aaagatggaa aactagacat tcagcaatat tgggatgcag
     6841 attttgaaac aaaagaaacg cattcccgac aagaatggat cgaaaaaatc gacgaaaccg
     6901 ttcaagcttc tattgaagca catacggtca gtgatgtaga agttggttct ttcttatcca
     6961 gcggggttga ttccagttat gtaacctctg tattgaaacc agatcattct ttctcaatcg
     7021 ggtttgatga taaaacctat aatgaagcga ttgaagccag aaaactaacc gaactattgg
     7081 acttagataa tacagcagca gtgatcgacg gagacatgtc gttcaaagca ttccctttga
     7141 tccagtatca cttagatgaa cctgattcaa atccttcttg cgtacctttg tactttttag
     7201 caaatcttgc ttcacaaagt gtgcgggtag tccaatctgg agaaggggcg gatgaactgt
     7261 ttgcgggata ccaaacgtat ggattccata cgaattcaaa attcatccgt gtgattgctc
     7321 aaggattgaa aaaattacca aaaggaacac gctataattt aggacgtaaa atcggaaaaa
     7381 tgaaaaattt ccatggacgg atccatttat atgaatcact tgctcctgca aaagaatatt
     7441 ttatcggtca tgctcgagtg ttcgaagaat cagaagcatc agaagtgtta acaccaaaat
     7501 atcaaacagc accatctgtg gatgaaatca tgactgttca ttacgagaaa acagaaggaa
     7561 tcaaagatga agtcaataag atgcaatatg tcgatcttca tcaatggatg ccaaaagata
     7621 tcttgttaaa ggcagataaa ctttctatgg caagttcttt ggaagtccga gtacctttat
     7681 tggatatcga agtcatgaaa ttggctcaac aaataccaag taagttttta ttgaatcaaa
     7741 acaacacaaa agatattttc agacaagctg cgaacaaaca tcttccggaa gaatggtcga
     7801 atcgtgtgaa attaggattc cctgtgccaa tcaaagcttg gttgaaagaa gaacacggct
     7861 acgaacaagt caaagcatta ttcgaagcag attttgcaaa agaatttttt gatcaagaaa
     7921 aaatcatgaa gttgctagat gaccatcacg aaggacgtaa agaagagcaa agaaaaatct
     7981 ggacaatctt cagtttcttg acatggtata aagtctactt tgtagatgaa acgattccgc
     8041 aagcagaagc aatcgactat gtgactgttt gatgaaaatc aaatatcaat aaaaaagagg
     8101 ttttctccta ccgtttaaca ctgagtgata agaaacaatt tcaaaaaaca gctcttcccg
     8161 tttcatagat attatgactt atggttcgga gttggcagag gaacaccatt gtttcgaaca
     8221 taagggggtt gtgacacgat tttcgtcaca acccctttac atatgttcaa taatcgattt
     8281 ctttttcttt tgtcccatct tttttataaa tgaacaaagt tgattctttg ttttgtgcta
     8341 cttgctttcc tttttcgatt gcttcttttt ttgtatcaaa atggttgctt gccttttttg
     8401 cccctttaga tttcacgatc cattgttctt cctcatattc tacgatcaca tctgaatcaa
     8461 gcagttttcc agcacgagga ttcgtatcgt gttcatccgt cttgcttgga tttttttctt
     8521 tttcgaaagt tcgtttttct gattcactag catcttcata ccattttttt gcttgatcaa
     8581 tggcaattgg gatcgcacgg ttatcagggt acccttcatc taataatgca tttgcgatgt
     8641 cgattgcttt ttttcttgtc aatttatcta agtttttcat tgatgcagga aagtccttca
     8701 tgttccatgg catattagta tcccccttta ttaaaaaaca taatctatat aaacaagtgt
     8761 aagggtattt atacaaaaag aaaaagattt tgtttgagaa gtaaaaccgt tgtatttatc
     8821 gtcaagtagt tttacttaac aaaatcgtga aaaacactag caatctagaa aaaatcatgg
     8881 taagataatc aagtctgaga gtcatcttag aggattcttg taaaaatggg agggaaaaac
     8941 atgcgcgtaa acatcacttt agagtgtact tcttgtaaag aacgtaacta tctaacaagc
     9001 aaaaacaaac gtaacaatcc tgatcgtttg gaaaaacaaa aatattgccc acgcgaacgc
     9061 aaagttactt tgcaccgtga aacaaaataa tgtttttaaa gttctcaatt ccttgctggt
     9121 caaagaattg agactttttt tatctcaaaa catctaaaat cataaggagc ggataatatg
     9181 tttatttcca tgtgggcaca agataaaaat ggtctgatcg gcaaaaatgg tttgttacca
     9241 tggcgattgc caaatgatat gcgtttcttt cgcgaacata caatggatcg aattttagtg
     9301 atgggaagaa agacctatga aggaatgggg gatctttcac ttccttatcg ccatatcatc
     9361 gtgttgacaa cgcaaccaaa ttttcgcacg aaagaaaatg cggaagtaat gcattcgatc
     9421 gatgaacttt tgtcgtatag tcaaacgatc tcagaagata tctatgtttc gggtggtagt
     9481 cggatttttc aagaacttct tccttacact ggtcggatct ggcgaacatt gatcgatagc
     9541 acgtttgaag gcgatacgta tattgggaat attgattttt cagacttcgc ccttatagag
     9601 gagtatgagg ggataacgga tcaagaaaat ctatatgccc atcgttttca aaaatgggaa
     9661 agagtaaaaa atagggttgc ttcaaaagaa gaataaaatc tatgcgtcta gtttgttaat
     9721 aagaaaaaaa gcatctcacg actaatcgtg aagattcttt ttttagttaa aatcaattta
     9781 gtttttcttt gatatcattg accatttcct gactggcaat aggtccgtta tatagccaac
     9841 tagattcagg tccgaattgc agtacatgat tgtctgtcac tgctttaaga ttcttccagc
     9901 ttggttcatt gaacatggca gcatctttgt cactgttcac aaggatgatg tagtcagcat
     9961 ctaattcagc tagcttttca gaggaaacag cagaccagtc agaagtggct tttttggaga
    10021 cctcttccac tagatcagga acaccaaatt ccagatcgtc gtatacgatt cttccacttg
    10081 accgattttt gctgaccata aaggctgagt tgttggtcac ccacaacact gcagctgtct
    10141 tgttctcgat tttatctgct agttcttctc gagttttctt gatggatttt tgataatcgg
    10201 aaatgatttt ttctgcctct ttttcttttg ccaaagcttt gccgacatct tttagttgtt
    10261 cttcccatgt gacatctgtt ccgtttttta caacataagt tggcgcgatt ttgctatatt
    10321 gttcgtattt cccgccttcg acagtagcgg aagaagaaat caacagcaaa tcaggctcaa
    10381 agcttaacac tttctcataa ggaaggtcat aagaaatcgt tgggacatcc tttaaatcat
    10441 cttgcagata atcttgaatg gaaccactgc caacagtcca ctgggcgata ggtttttccc
    10501 ctagtgcaat cagatagtct tctaagtaac tgccgatgat ccgtttgggc gcgtttggaa
    10561 tctctacttt atgtccaagt gtatctgtga ctgtatgagt actattggat gaagtcgtcg
    10621 aattggcagt tgtagctgtt tctttatctg aagaggggga gcaagcgctc agtactccag
    10681 ccagcagcaa tatgctagat aatgtgaata cttttgtttt taatttcatt tctttcctcc
    10741 taacataatt gagaatcttt ctcattggtt attattcctg atatttcgaa cgctgtcaac
    10801 cgcttcattt ggctatagat atgaaagtag cgcttgacga aatgttgaaa cagcgtcata
    10861 attgagaatg attttcagcg aaaggatggg tgaaatgacg agattaagag cagctgacat
    10921 tgcagccggc tacgaaaaca aaaaaatcat tgaaaacatg tctgttacga ttcctgaagg
    10981 gaaaatcact agtttgattg ggccaaatgg aagtggaaaa tctactttat tgaaaagttt
    11041 tacccgtata ctacctgtga aatcaggaaa gatcattgtc aataactctg atatctctac
    11101 atacagttca aaagaacttg caaaaaaaat tacattatta gcacaaacta gtgaacaccc
    11161 tacaggtatg acagtagaag aagtagtatc ttacggacga tacccttatc aaaaactgtt
    11221 cagtggcatg gacacgcaag atcatgaagc aatcgagtgg gcgttggatg ctacaggtct
    11281 agctggattt cgtatgcggg atatcgcaac gctgtctggt ggacaaaccc agcgtgtctg
    11341 gatcgctatg gcgcttgctc aggaaacaga tatcttgatc ttggatgaac cgacaacatt
    11401 tttagatccg gcacaccaat tagagatcct acagttattg actgaaatca atcaaatgaa
    11461 acaaacaacg atcttgatgt cgattcatga cattaatcat gcttcacgtt tttcagatta
    11521 tttattagca atgaaaaacg gtaagttgat cacagaaggt acacctgaag aagtgatcac
    11581 aaaagagtgt atgaaaaaaa tatttgagat tgatgcaacg attgtcacgc ttccaagaac
    11641 aaataaacca gtttttctaa cctatgattt gttgccaaaa gagcaggagg atcaattatg
    11701 aggactatga agaaaaatag actcctgctt ttggtctgtt ttgttctttt gattattttt
    11761 tttgttgctc tttcaaatgg cgcagcgact gtgggaaaag cagatttttg gcaggcgttg
    11821 tttgattttg atagatcaaa acaaagtcac caaatcatac gaaatattcg tttgcctcgt
    11881 gtggcgggag catttcttgt agggagttgt tttgcattga gcggtgcatt gatgcaagga
    11941 gtgacaagaa atcctttagc agattcaggc ttactaggaa tcaacgcggg tgcttcgttg
    12001 gcaatggccc ttagttttgc tttttggcct aaagcttctg atttttctgt gtttctaatg
    12061 tcattggcag gttcttttgc tgtaactttg ctcgtatttg gttttttgca gttttcgaaa
    12121 atgggcatga atcctttaca gctgatttta gctggcgtag ctattagttc tttttttact
    12181 gctatcagcc aagcacttac ccaattcttt aatctgcagc aggatcttgc tttttggttt
    12241 gtaggcggag ctgccaatat cacatggaaa caattagcag tattgtttcc tttatatata
    12301 cttgctgttt tggtttcttt ttcattagga aaagcaatat ctttgattca tttgagtgaa
    12361 acacatgcat tagctcttgg aagtcatcct aaacgaacgc ggttgattgt tttctgtcta
    12421 gttgcagtgt tagcagccat tgccgtttcg ttagtcggac ctgtttcatt tattgggctg
    12481 ttggttccgc cagttgttcg cggaatggtt ggaacggact atcgttatgt tctacctgtc
    12541 tcttttttag ttggcggtgg attggttatg ttggcggatt ttacagcgag gatgatcaat
    12601 cccccttttg aaacaccttt tggattagtc attgcattga ttggcgttcc gtttttactg
    12661 atacaagtaa ggagggaaca acggtgaaaa aaatatattg gacgttctat ttcttgcttt
    12721 ttgtacttgt catcctgtct ataggtagtt taatgattgg tacgcctttt atttacctgg
    12781 atcagctttt tgccacgttc atagggaaag aatcatcttc tttggcgctg attgttagag
    12841 aatttcgagt tcctcgatta ctgatcagtt tattagcagg agcttgtctt ggcgtttctg
    12901 gttatatctt gcaaggagta acaagaaatg aattggcaga ttctagtata ttgggcatta
    12961 atgccggagc aggattttta gtgatgcttt atttaggttt cttttcgcac tattcgctgc
    13021 cgtttctatt gctagttgta gcctttctag gtggtgtttt agctgcttgc tgtgtctatc
    13081 ttgcggccta tgatcgaaat ggctttttgg gtatgaatcg aatcttattg tccggtattg
    13141 ccgttaatgc cggcctttct gcattaacat tgctatgcac gatccaatta tctaaggaaa
    13201 attatggatt tgtgaatgca tggttagcgg gatcaatctg gggtgcgagc tggccttatg
    13261 tttgggcatt gcttccatgg gcggttatct taattccgct aggaggactt tatgcacaga
    13321 ggattggatt gctatcattt ggtcaagaac gcgcgcaagg attaggcctt aacgtggcta
    13381 aaagtcaaaa aatccttttg ttgttagctg ttgctttggc aagcggtagt gtagcggtga
    13441 caggtagttt atcttttgta ggactactag ctcctcatgc tgcgaagcta gtcgtacgta
    13501 aagaaaataa ctggaccttt gttctgagcg gtttgttcgg ttccattttt ctgcttagtg
    13561 cagatatggt tgctagagtg attctaccaa gtggggaagt acctacagga attttgattg
    13621 ccctttttgg tgcgccatat tttctgtatg cactttttat cagacaaaaa cacgcgctga
    13681 acagttgaaa tttattaaaa aaggaaggcc aatcatttct aggtcttctt tttattctat
    13741 ttatgcattt atttgaaagt tcgattgcta tttttctttt tttcctatac cattttatga
    13801 gagaaggaag ggtgagtttt tggaccaatt gaaagtaaga ttacgccgga aaggaatcga
    13861 aaagctgaaa aatctggcca aaagtggcaa gaaaaaggaa aaagaacagc gaatctataa
    13921 acagctattc gccagcgagt actggaagtc agcaaaagtg atagggacga ctttatcgaa
    13981 tgaatttgaa ctggatacac agccggttat ccaacgagca gaaaaagaac aaaagaaaat
    14041 agttgtacct aaaactttgc cgaaacgtca gatggcgttt tatgaaatgg atgaacagtc
    14101 cgttcttgta cgttcgagtt ttggtgttct tgagccacaa tcagagaagc tctatttgcc
    14161 ggagatgatc gatctcctca ttgtaccagg tattatctac aatagagccg gttatcgaat
    14221 cggttttggc ggtggttatt acgaccggta tttaaataat tttcataaaa aaacatgtag
    14281 ccttgttttt cgtgaacaat tgaatgaaca atggcaaccg gagagctttg atcagaagat
    14341 ccaacaattg tttattgact aaaatgaaat gggaggtttt catgaattat caacagcaaa
    14401 taaaattgcg tgcatggtta agacgaccgt tatggaccta tgctttttta gggatacaga
    14461 cagcggtctt cctcatgatg gaattatttc cgcgacttga gatcccttat tatgccggaa
    14521 tgtatggtcc ttatcttgtt cacttcaatg aatggtggcg tttagtgaca ccaatcttca
    14581 tccattttgg actgatgcat ttcgtaatga actcactgat actgtatttt atggggcagc
    14641 aaatcgaggc aatctatggc cattggcgtt ttttcttgat ttatatgttc agcgggataa
    14701 tgggaaatac agcaagcttt gcttttaacg aagcgaatgt tctgtctggt ggtgcgagta
    14761 cttcgatatt cggtttgttc ggtgcgttgt ttatcttagg gtttcatttt aaacataacc
    14821 cagcgatcca gcagctagtc agacattttc ttttatttat tgtgttgaca tttgtttttg
    14881 gtcttctgga tacctctgta gatgtttggg gacatgtcgg tgggctaatt ggcggattag
    14941 tgttagggaa tgttttgggt ctaccgaaac aacagacaag ctattcgata caccaaagga
    15001 ttctttctac actagttttt gtatttcttt ttgtaatatg tattttactg ggtttaaaaa
    15061 aatatggttt acttgtataa tatgaaggaa gtgcttaaag atggagagct tatatgatgt
    15121 acagcagctg ttcaaaaaat ttggcatctt tatctatgtg ggtgcacgca tctatgacat
    15181 tgaattaatg actatcgaat tgcgtaatct gtatgatagc cgtttgattg atcatgatac
    15241 atacatgaca gcgtggcgca tattaaaaag agagcaccga attgaagaaa gccgaaagaa
    15301 aggaaatttg taatggacaa gaagattatt gggattgatt taggtggaac aacagcaaaa
    15361 tttgcaattt taacaccaga aggagagatt caacaaaaat ggagcatcga taccaatatt
    15421 ttggatgatg gtaagcatat cgttcctgaa atcattgaat caatcaacca tcgtttgaat
    15481 ttatataata tgaaagccga agactttatc ggaatcggca tgggtacccc aggaagcgtg
    15541 gacagcgaag ctggaacggt tattggtgcg tataacttaa actggacaga agttcaattt
    15601 gtgaaaaaac tgatcgaggc aggaacagga atcaaattcg ctatcgataa tgatgcgaat
    15661 gttgctgctt taggtgaacg atggaaaggt gctggagaaa acgatccaga cgttgtattc
    15721 gttactctag gaacaggtgt tggcggtggt atcgttgctg gcggaaattt gattcacggc
    15781 gtgagcggag caggcggaga gatcggccat atcactgtgg atcctgaagg gttcgaatgt
    15841 acttgcggta aacgcggatg tttagaaact gtgtctagcg ctactggtgt ggtacgtcta
    15901 ggtcgttact tggcagaaga atacgcagga gattcaaaac tgaaagcaat gttagacaac
    15961 ggtgaagaag taacaagtaa agatatcttt gaaatggctc aagaagacga tccgtttgca
    16021 ttgatggttg ttgatcgcgt atgcttctac ttaggtttag cttgcgccaa cttggggaat
    16081 acattgaatc cgtcaagtat tgtgttaggt ggaggagtat cggcagcagg agaattttta
    16141 cgtagccgtg tagagaaata ttttaaagaa tttactttcc cgcaagtaag agaatcaaca
    16201 aaaatcaaat tagcagagct tggtaatgaa gctggtgtca ttggtgctgc ctcattagcc
    16261 ttgaaatttg cttaaaaaaa cggaggcatt acatggtttt atgggtaatt aatatcattt
    16321 tgctattgat tgtgcttgca atcatctttt gggaagtcta tctacgagta atggccaagc
    16381 gttcagcaac gactttgaca gaagaagaat ttcgagaagg aatgagaaaa gcacaagtaa
    16441 tcgatgttcg cgaaaaagat gtgtttgatg ctgggcatat tttaggtgca agaaacatcc
    16501 cttacactgt tttgaaagat tcattaggat ctattcgaaa agaccagcct gtctatattt
    16561 acgatcaaaa gaaaagcttg agcatccgta cagcaaataa attacgtaaa gcaggttatc
    16621 aagagattta tttacttaag ggtggatatg acggatggtc tggaaaaatc aaaagtaaaa
    16681 aagtataaaa cagatgattg tatgacagct ttaaaaaccg aatcaatcat tagattttga
    16741 ccaagatcac atgaacggcg caatggatag ttgtgccgtt catttttttt attttgtgct
    16801 attatctaca tatataagct aagcgaaaag gcagaaagaa ggaaacaact atgatcaaat
    16861 taaaacgtgt ttaccttgat gctgaaaaag aggatggcta tagaatactc gtggataggt
    16921 tctggccacg aggaatttca aaagaaaaag cgcagttaga tctgtggttg aaagaaattg
    16981 cacctagtaa tgaagcacgc aaaacttttg accatgtacc agaacgattt gcttcatttg
    17041 caaagaaata ccagcaagaa ttgattttcg aagaagaaaa gaagcaagct atttttaaac
    17101 tcagtcaact tatgaaagaa catccagtca ttacttttgt ttatggtgca aaaaatgaac
    17161 atcagaacca tgctgtttta ttgaaaaaat ttatcgaaat ggaggcatgc aaatgaaaat
    17221 agggatcatt ggtgccggac ctagaggatt aagtatagca gaaaggattc tccataataa
    17281 tcaggacaga cattctcttg aactttttct tttcgatccc aatggtcctg gaggtcgagt
    17341 ttggcggttg aatcagccac cggagttatt aatgaattct gtttctcaac aagtgacgct
    17401 ttttacagat gaaacattgt ccagtggagg attggtaaca cagggaccca acttgtttca
    17461 atggagtaaa ggacctgcta cagattatat cgaacagacg gtaaccaaaa ataagcaact
    17521 aattatagaa gaaatcaatc gtctggaaaa agatcagcaa tctactcgat gtttatacgg
    17581 actctatcaa cgctggtttt tctcaaagtt gcaagaagaa tttccagctt ctattcagtt
    17641 tgtttattct ttagtaaaaa aagtcagcaa aagaaaagat ggttatcttt tacagacaga
    17701 tcagcaaagc tattctatcg atcagcttgt tttagcaagc ggtcactggg aaaatgatga
    17761 gacagaagaa gaaagagtgt gggtagacta cgcaaagaaa aatgatttac tgtatcagct
    17821 tccttctaat cctgcagatg tcgatctttc gaagataccg cctaaagaaa atgtcttttt
    17881 aagaggtcta ggcttaagtt tttttgatta tgtcggattg tttacagtat caagagaagg
    17941 gcgcttcgag aacgaaaaag gaaaacttgt ctatcatcca tcaggcaagg aaccgattgt
    18001 atacagtgga tcaagaaggg ggctccctta ttaccctcga ggaagaaacc aaaagcaagg
    18061 cggatcaatg gcatggccac ggttactaac aaaagaaaac ctggagcaat ggcatcagtc
    18121 gggatttttg actggcgaaa cattttttga ctatctgaaa aaagacgttg agttgtttta
    18181 ttacaagaaa atcatcgaag aatatcattt gccgattcca aagaaaaatt ttgagcatga
    18241 ttttctctcc ctctcttcag aggagtgtct cggaaagcat ccagcactaa tcccttatag
    18301 atgggattgg tcgtatttag aaattcctct tacctatcag gatgagtcct ttgcagaagc
    18361 tagccgagca tttattgaag ggcagataaa agaagcggaa aaaggcaatt gcacaggagc
    18421 atttgcttca acatttgatg cattaaaaga ctggcgtgat ccaatccggc aggctattga
    18481 atggcacgtt ttttctgcaa aggaatacaa agaaatacta tggggatggt ttactcatct
    18541 aaatagcttt ttaacgatcg gtccgccagt cattcggacg aaagaattag cagcactgat
    18601 agatgcgggt atttttcatt tgttcgaacc gccatttgat attcagcaaa agcacaaccg
    18661 ctatgttatt tctctacaag ataaagaaat cagcagtcgc tatttgattg aagctcgatt
    18721 gccacaaaca agcttttctc gttcaagtaa tcctgtcata aaaaatttga aggaaaacaa
    18781 acttgttcgc cctttttcat tttcagataa tacaggaagt tatcattcag aagctataga
    18841 tgttgagcga ggtacgaatc gcgtgattga tgcttcaggg gaaatccagt ctgactttta
    18901 ttgctatgga atacctgttg aaggtgtgga ttggctaact gcagcagtag ctagacctta
    18961 tacagatcca tggaatctga gacaggcaga taaaatagca caacttatat tgactgacaa
    19021 ataaaaatac gcatgatgac ttatctgttc tttcacaagt cttcatgcgt gtttttaaat
    19081 aattattaac gagaaacgcg tttgcgcatt gcttttttac ggttttcttc gatcattgct
    19141 tctttttcgt caattggatc gatcactgtt tttttcacag aagcgaccaa accagcaacg
    19201 gcagcggctg ttgccagact tcctactaat attccagata caaatttttt cattgaatcc
    19261 acctcattta ttatttgtta tatctattat ggaacaaaag agaaaaaaag gctagtttta
    19321 cgtttgtttg taaacctttg caaaaaatcc ttgaaaatgt ttactaaatg ttttattata
    19381 aatttaccat atg
//
LOCUS       DAXWLG010000040        18824 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXWLG010000040 DAXWLG010000000
VERSION     DAXWLG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.040"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(58..921)
                     /locus_tag="ACGE4T_001125"
     CDS             complement(58..921)
                     /locus_tag="ACGE4T_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFF8386419.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1117..3216)
                     /locus_tag="ACGE4T_001126"
     CDS             complement(1117..3216)
                     /locus_tag="ACGE4T_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFF8386420.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3436..4545)
                     /gene="rpoD"
                     /locus_tag="ACGE4T_001127"
     CDS             complement(3436..4545)
                     /gene="rpoD"
                     /locus_tag="ACGE4T_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFF8386421.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4569..6410)
                     /gene="dnaG"
                     /locus_tag="ACGE4T_001128"
     CDS             complement(4569..6410)
                     /gene="dnaG"
                     /locus_tag="ACGE4T_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFF8386422.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6592..8907)
                     /locus_tag="ACGE4T_001129"
     CDS             complement(6592..8907)
                     /locus_tag="ACGE4T_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8386423.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9092..9952)
                     /locus_tag="ACGE4T_001130"
     CDS             complement(9092..9952)
                     /locus_tag="ACGE4T_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFF8386424.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10011..10643)
                     /locus_tag="ACGE4T_001131"
                     /pseudo
     CDS             complement(10011..10643)
                     /locus_tag="ACGE4T_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            10810..11571
                     /locus_tag="ACGE4T_001132"
     CDS             10810..11571
                     /locus_tag="ACGE4T_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8386425.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11597..14188
                     /gene="mprF"
                     /locus_tag="ACGE4T_001133"
     CDS             11597..14188
                     /gene="mprF"
                     /locus_tag="ACGE4T_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8386426.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14376..15563)
                     /gene="tuf"
                     /locus_tag="ACGE4T_001134"
     CDS             complement(14376..15563)
                     /gene="tuf"
                     /locus_tag="ACGE4T_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8386427.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15931..16287
                     /locus_tag="ACGE4T_001135"
     CDS             15931..16287
                     /locus_tag="ACGE4T_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8386428.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(16528..17532)
                     /locus_tag="ACGE4T_001136"
     CDS             complement(16528..17532)
                     /locus_tag="ACGE4T_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFF8386429.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            17698..18549
                     /locus_tag="ACGE4T_001137"
     CDS             17698..18549
                     /locus_tag="ACGE4T_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8386430.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            18566..18811
                     /locus_tag="ACGE4T_001138"
     CDS             18566..18811
                     /locus_tag="ACGE4T_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386431.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         5241 a         3882 c         3311 g         6390 t
ORIGIN      
        1 aacactatgt taattaaaat tattataagc tagttaagaa aaacatgcaa gggattctca
       61 attagatttt ttgactagtt ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa
      121 aagattacct aatgcgcgct taaatgctcc tttgctgata ccaaaagctt tcatgatttc
      181 ttctggatcc gatttatctg taaagttgat ttgctgatct gcagaacgtt ctaaaatggt
      241 taaaatcatt tgtgcatcat ctgatatagc ttcatatgct cttggcttca atgaaagatt
      301 cagtacaccg tctggtcgga caccaatcac tcgacctttt atctgctcgc ctagacgagg
      361 ttcattatat cgttcagaag gatggataaa cccaagatag aaatcttccg ttaaaacgta
      421 cgtacctgtt aatttcaagc ggtatgctgt tcctatcaca tctttgtttt tcatttcttc
      481 tgttccatat ttgcttaaag atttgaatat tttttcatct gctaaactgc cccaaatacg
      541 gtcttttgca tcaactctaa gggaaatcat caagcgatcc ccttttttag gccatagctc
      601 tgtcattgtc ggcaattcat caagagatac gacaaagtct ttatctggaa gaccgatatc
      661 gacaaacgca cctaaatctt ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat
      721 acggctttta ggaatttgtg tagtgatccg attttcatgt ttttgatttt ggtaaccgaa
      781 tccttcaact gcttcaccga ttttgtgttc accttctgat ttgcttaagc caaaggtttg
      841 tccgtttttt tggacaaagt agtttttttc attttcatca ataataatac ctgtaaatat
      901 agttgctaat aaatcgttca tttttttctc cctctatagt gaaaggtttt cttaatacat
      961 ttctttgcta tcataacata atgttatcta cttgaatagc tgagccttgg tttggaaagg
     1021 tgaaaaaagg agtatgacaa cagttgtgtc atactcccct ttttcgaata aacggtgtta
     1081 aacggcaggc tcacagcctc tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa
     1141 ccatccagtt ttacagttga tggttacgat gtaaaatggc aaagcacttt ctccaatgcc
     1201 tttcatcaat gcagattttg ttgaagtggc atatagatta taatagcctt ccccattttc
     1261 gattctggct ttttctaatc tgtaacctcc ttcaacgata tatccacgtt ggatacatgt
     1321 agcaatcact ttgctttgga cacctggtgc ccacgtccat gctggattag atgcatcggc
     1381 aatatcactt gctctggcag atttgattgg ctgattcgct gagtttgaag aggaaggggt
     1441 cgaggatgcc gcttcttgct gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc
     1501 tgcagctgct gcttgagcgg cttgttttgc ttgttcttca gctgcttttt tcgcttcttc
     1561 tgcttttttc ttttcctctt cttctttttt ctctttatca gtgagcatct tatctacttg
     1621 tttcaattgt tcattcaact gagcagatag atctgtattt ttcacttttt ctactgcttc
     1681 ttttgccgtc ttgtattggt cgcgtgttgc tgaatccact acttttccgt ctttaaagac
     1741 tatagctgtt ttttctttag ctgtttgcaa ttcagcatat tgatttttag cttctgtttg
     1801 tgcttgttga atcaattttc caaaatctgt gttatccgtt tcgattgctt ggatctcttt
     1861 atctgctttc aacttgatgt catctgctag atgatcatcg acgatggcag gtgctatgaa
     1921 ccactcattc actttttgga tatctcggat cttagattgg atatcttggt aagttgtttc
     1981 taactctgaa tatccttttt cattcttaag actggataaa tcatttttta gttttgataa
     2041 atcttgatga atcatatctg cacgaataaa ttgatggtga tcatctgtat aaaaggcagc
     2101 aagtttctca tgaagttctt ccatttgttg ctgtcttttt gcttctgctg ctacttcggc
     2161 ttctactttt tgatgatggt cataataagc ccagcttcct ccaccgagta gaagtactgc
     2221 tgctaaaacg agataaggta ctttttttga ttttttcttt tgcgctttat ctggatctgt
     2281 tttttctaag tttgattctt gatgtgtttt ttcagtatct ggcacgattg ttattgattt
     2341 cgcttctgct tcaaacagct taggctggtt ctcttctttt tctatttttg cttcaagatc
     2401 agtaccctct ttgcttacag attgattttc agcatcggat tcactattta tttcatcggg
     2461 ctctgttgac tttttgtctg tgttttcgag tatttctttt ttctctgctt ccggcagttt
     2521 attcgttttt tccttttcta attgtttttc agtaaatact tcagtttttt ctgtgatatc
     2581 cgtttttacg tcttttattt ttactgtttc tttacctgtg gcgttttctc tatcagagtc
     2641 tttttcagtt tcttgttcct tttctggctc tactttgttt tcctgtgcaa ttttttcact
     2701 gatcattttg agcagctctt tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat
     2761 ataagcggat agtatcgaat tttcttcctc attatcgacg atttctttca gttcagctac
     2821 tcttcttttt tgttttgatt caagcgtttc ttcttgattc tttcctgatt cttccgaagt
     2881 ctcatgaaca gttgctttct tctgttcatt tttctctata ttagttactt gatcgtgagc
     2941 aggtggttct tcgtcttctg gttctgcttt ttcgaataat tccattacag attctagagg
     3001 taaatctttc aattccgacc attcgattgg atcgtttaat tctgtatcag gaaagactgc
     3061 atcttcagtt gatttatcct gcatagctgc atctgtattt gtttcttcaa ttgattcttt
     3121 tatttgctga tcttctgcag ttatttgatg accgcagtct ggacagatag tattctgctc
     3181 catttttttc gatccgcaat ttggacattt tttcatcact tagcgatcgg ctcctttact
     3241 aggtttacgc taccatttaa tctatcatat tctatacgtc acgccaatag cttattttta
     3301 ggatttactt aaaaataatg aatgaatttc cttcctatag aaaaaaaagc ttgagactgc
     3361 gtagtctcaa gctttttttc ttttaaaata aaccaaacag ttttactgat ttctcttctg
     3421 gtcaatagtc cacgcttatt ctaagaaatc ttttaattgt tttgaacgag aaggatggcg
     3481 caattttctt aatgctttag cctcgatctg acgaatacgt tcccgagtaa caccaaatac
     3541 tttccctact tcttccaatg ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag
     3601 gacattctct tcccgatcag tcaatgtatc tagtacatct tccaattgtt cttttagtaa
     3661 ctcataagct gcgtgttctg ctggacttgt tgcttcctgg tcttctatga aatcacctaa
     3721 gtgagagtca tcttcttcac cgattggcgt ttcaagggaa acaggttctt gcgcgatttt
     3781 caagatttct cggacttttt ctgttggaag atccatttcc gcaccgattt cttctggcgt
     3841 aggttctctt ccaaggtctt gcagcaattg acgttggata cgtatcaatt tattaattgt
     3901 ttcaaccata tgaactggaa tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat
     3961 ggcttgacga atccaccatg tagcataggt tgaaaactta aatcctttgc ggtagtcaaa
     4021 tttttcaact gccttcatca gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat
     4081 gcctcgtcct acataacgtt tagcgatact tactactaaa cgaaggtttg cttctgccag
     4141 acgctgtttc gcttcttggt cgccttcttc gattttcaat gctaacgcta cttcttcttc
     4201 cgcagttaaa agggagacac ggccaatttc tttcagatac atacgaaccg ggtcattgat
     4261 ttttacaccg gttggcgcag aaagatcttc tgtttttgct tgttcagctt ttttctcagc
     4321 actcttcaag ctgtgaattg atggatcacc attttcatcc accacactga tacctgcgtc
     4381 ttcaatcttt tggatcaatt tgtccatttc atctgcattc aaagtgaatg gtgtagcaag
     4441 ctgattggat aaatcatcat atacaacttg tcgtttgggt ttgttttctt tgatgaaagc
     4501 tgctactgct gcttcatacg tttttttatc tgttccattt gccataaaag aaggcccccc
     4561 ttgagtagct atttggcttg ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat
     4621 tcaagctcaa gctgtttgtt tccagttcgt ctggcttctt gctgcatttg tttttttaat
     4681 gttatttcat cagctaagcc agatttgctg atcacatcta gcaagtcttg gatttctcgt
     4741 ggcgaacttt cttctgccac cggtaaatat tcgatttcca ccaccaaccg ccttaaattt
     4801 tcatcttgca aaaagtctaa aaactctgca aggttgaatt cataatgttg tgagacatat
     4861 gcatcgaata aaaaataaac ttctgcatat gcatcatgaa taaactgaaa ttctcgttct
     4921 tttaaaatat tacggacaga aacttcttta aatgcacgat ataatagaat ctgctctgcc
     4981 ttttctactt gcgtcaaagg tctctttttc cgtgcagttt cttgtctcgt aacctgagtg
     5041 tgttgacttt cctgaggttc ttgacgattt tgctgatttg tccttcgaag atcgcgcagt
     5101 tgtttttgta tcgtctctct gccgatctga aactctgttg acagttgaga tagatacata
     5161 tcttgttcga ttggtgaaac aactcggcta agttcaacta gtaattcttc gaggtattcg
     5221 atcttgtctt tttcattatc taaatttttc ccttgtttta aataacgtga tttaaaagta
     5281 aagactgttt cgcgcccatg agccactaat tcttggaaag atgcttctcc atacttacgg
     5341 agatattcat caggatcaag cttttctgga acgactacga cagaaagtgc taatcgactg
     5401 ttttgccgaa gcaattcaat ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca
     5461 tagcaaaaga ccaattcttt cgtcagcttt tccatttgtt ggatctgttg agctgttaga
     5521 cttgtcccca tagaagctaa tccagttttg ataccagcct gccatgcagc aatcacatcc
     5581 ataaaccctt cgaacaacaa aatttctccg tttttccgga tatcgcttct tgctttatca
     5641 agattaaata aaacttgtct tttattgaac agttctgttt ctggactatt caagtatttg
     5701 ggctgatctt tttcctgccc tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc
     5761 tggccttgag aattacgaag tggaaacatc actctttgat aaaaacgatc agacatacgg
     5821 ccatcatcat gcgtgacaaa caatccagag cgttctaaca attcatttga aattgattca
     5881 ttcttgaata cttgctgaag aaaagaacgc tcattaggtg caaaacctat gttgaattct
     5941 tcaattaatt ctaaagacaa cccacgactt tgcaaatatt ctaaagctgg ctgtccagct
     6001 tttgtatgca atagcatgtg atgatagatt tccgctgctt tttcatgagc agcaatcagt
     6061 ttccccgtag cagattcagg attttggaca gctatagtac gctgacgcca ttgatcttct
     6121 aacggtatct gttcgaagtc tgccactttt accactgctt ctggaaaact tagaccttct
     6181 agttcttgaa gaaaagtaaa cacgtttccg cctttaccac aaccaaagca atgaaaaatc
     6241 tgcttgtctt ctgcaacaga aaaagacggt gtacgttctt catgaaaagg acacagcccc
     6301 aagtaatttt taccagactt tttcaactgg acatattgtc caatcacctc aacgatattc
     6361 gtttgactgc gtactgtttc gataacctct tgaggaatcc gttgagccat tttttcaccc
     6421 cctgctctat tgatatcttt tgggtttaac agaagatatt tcaggagaat aaccggtaaa
     6481 aaatcgcact aactgaaatc agtgcgattt taaaggacac aattatacat gtttgattgt
     6541 atcatatttc attgcttttt caagtataac acatttctat ctcattaagc atcaatttgc
     6601 tactcttttc agtaactgat tttgaatatc agccttccag tacaatactc gaagaacaat
     6661 atttacgagc atgaatacaa agaagtatcc tacgctctct cccaacccta agtttacttc
     6721 caaaattcca tggaacagca ttgcagctac atatatccct acgatcgtcg ttactgctag
     6781 gaagatgcgc aatgggttga agggataaca agcttttaca actgccatgc aactgatccc
     6841 aatcagcaag taatacatca aagtagtagt ttcagcattt ccccatgcga atgtttttcc
     6901 aagtagatac accgcaatga tattcactac tacaagcaag gcatttggta aagcattaat
     6961 aagtgctgta ggcaaataac ggtaagtgat ttttctttta tcttgttcaa aagataaaaa
     7021 gaaggaagga tacccttcaa tcgctaaatc aatcagcgtg atttggatag ggataaacgg
     7081 aaatccaatt gctgtaacgg cacatataat cgataaaata aatgaataaa tcgttttgat
     7141 aaaaaagatg cctgagactt tcgtcacatt gttgaccact cggcgtcctt caaacaggac
     7201 ttctggcaat gtagtaaaat cagagtccaa caataccaga ttcgcaatct gacgtgttgc
     7261 tccatcacct tctgccatag caatgctgca atctgcttct cttaacgcca aaacatcatt
     7321 cacaccgtcg cctgtcattg caaccgttct gccgctttct ttcaattcat ttaccagcaa
     7381 tttcttttgc tgcggactca ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac
     7441 ttccatttct tcagtaattt gagaaagatc gacatacgca tcataattcg gtaatcctgc
     7501 acgtctagcg atattggaga cagtaaaagg attgtctcca gaaatcactt tcagatccac
     7561 accttcctca tgaagataag ctagtgtttc ttgagcgttt tttcggattg gatcatcgat
     7621 ttcaaacaga gcaaccggct caacaaatgc tggtttttct tcgttcaatt gctgatttgg
     7681 agcgatcccc aacataagta cgcgatagcc attcgcttgg gctgttttta gactgtccgg
     7741 aagccgatct tcttccacca aacgttcagg cgctcctacg tacacgactc ccagttctgg
     7801 aaattccatc gcgccccatt tgcgatcaga agaaaatgcg actttgtttg tcacatcata
     7861 ccgattagaa agctcaaaat aatctctcat agcccgcata gtgatgttgt tatcactgct
     7921 ttctgccaga tacgtcccca taatttctgg gatggctttc tcgtacagcg gatgtagtgc
     7981 ttctacccgc tggatactca ttttcccttc agtaatggtc cctgttttat ccaagcataa
     8041 tgtatcgaca tgggccagcg tttcaattga atacatgtct tgaacaagga ttcgtttttt
     8101 tgccagtttg gttacccctg tcgttaatgc aatacttatt agcaatacca aaccctttgg
     8161 aagcatgcct aacaaagctg ctgcagatgc aataacagaa attttgatag aagcatcgcg
     8221 gatgaacagt gcttccaaaa atagtatgat ccccaaagga ataatcacga aactagtgaa
     8281 tttggatact ttgcgaatcg aatctacaag ttcagaacga atagctttgt gagtctttgc
     8341 ctcgctagtc aagcggacag cataattatc tgcacctact cggataactt cagccatgac
     8401 agaaccgctt gtcaaaaagc ttccagaaag caattcagct ccagtttctt tgacaatcaa
     8461 atcagattcg cctgtcaaaa gagcttcatt tgcttctgcc cgtccagata taacctgtaa
     8521 atcggctggt atctgttctc ctgctccgat ttttaccaaa tctcctaata ccagctcttt
     8581 agtagaaatc gattgttctt taccgtcccg gatcactagt acttctgatt gggaaacaat
     8641 cgataatttc cgtacaagat ttcttgcatg tatttcttga taaattccaa taacgatatt
     8701 gacgacaata attgctaaat acgccatgtt ggaatacgca cctacaaata acagacaaat
     8761 cccaatacca aagttcagaa aattgaataa cgtcaatata ttatcgctga atatttgttt
     8821 tgtacttttt gatgcatctt cctggtagtc attttgctgg ccagaagcca ttttctgaac
     8881 tacctcttgc tgtgtaagtc cattcatttt ctttttctcc attttccgcc aactttcaaa
     8941 aataatcacg tgtttaagtc tagcgtgttc caagattaaa aacaacttgt ttttataaaa
     9001 tttatagaaa aacacacgaa tgattttaca aaaaagatgc caaccttttt gtttgaatca
     9061 cccgtgtgct gtttttatta tcaatctatc tttacttata ttgttcctcc aaacggctca
     9121 tccaccaacc tagtgctgta ccggctataa aaagaacagg acaagctagt accccagcaa
     9181 ctcccaatcc cgcgtaatcg gtaggaatga tcataatcgt tgaagcaaaa actacaccta
     9241 aaatgaaatg gaatagcttg ctatatgcct ttgagaaaat gtaatccatg atttttgaca
     9301 atccaagaac acaaagaatc ccaccaattc cgatcggcac aatcacttca agtcgtactt
     9361 ctttaaatcc gtcagccatc gctttataca atcccatata caccaaaaaa ttagaagggc
     9421 tcaatcctgg gacgatcata cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa
     9481 aattctgatc gacttgcccg tcaaataatt gagcgccgta taataaaaac aaataagcca
     9541 gaacaaaagt aacaaccatg atgatcacat cagaagtatc tcttcctttt ttccctgctt
     9601 ctttccataa cgcaggaaca gttccagcga tacatccgac aaaaaaccat aatatcgttg
     9661 tttcatacgc acctaataaa aaactgacta aaaaagaaag tagaaagatg cctgtcactc
     9721 cgcctagacc aacaggcaga aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg
     9781 ctaaaaaaga aatcagccgc tcatacatgc caaagacagc tgctaatgca ccaccgctga
     9841 ctcctggcaa gataaatcct gaaccgataa acattccctt cacaaaccgt aacatccagt
     9901 ctttggttgt gtaccccact tcttgatttt ccattttttg ccttttttcc actatttgcg
     9961 cactccttct ttcatttaat tttattcatg attgaacatt tcctgctatt ttacttaaag
    10021 actttttcat aggtctctat tattttttca ttcattttga caatatcagg ataaaacatg
    10081 ttacctccat tatgatagag atagccgcca ttgtaaagca aggactggat ccgccaatat
    10141 cggtattgtt tcccttcctc atttcctaaa agtgggctca gcacatcttt agaatacgct
    10201 tctgctaatt ctactgtgtt ttctccgcca tgatttttta cataggcaat ataatctttc
    10261 ccaaaattat atgcttggac agctgtccat aaatcgcatc cttgtttctt tgcttctact
    10321 atcatttccg ccaaatattt gacaccttga tcaatactgt cagctggatt atcaatgatc
    10381 tcagcttctc catgcagact ttccgagctc tgcatgagat ctgtcccttt tccttttgat
    10441 tcggtcaata ttatcgcctt gatcaaattc tcgtagtttt ccatatgata attttttgca
    10501 gctgttgtta tttctatttg atagctttcc actcgtttta ccgaacgata agttcttcct
    10561 cataaaaaca acaccgcacc aataaccgtc agaatgatca tgtaaaaaag aatacggata
    10621 atagataatc gttttcgctt catttcatca cctagttatt ttgacatctt tttttatctt
    10681 actatttctt tatcttcttg gctatttctt ccttgatttc tgtgtacgcc ttaagaaaaa
    10741 atgaattttc ctgtattttt tgacatctta cgaaaatgat cgttgttttt cagtaaaaac
    10801 aattgcccaa tgactgatat aatcgttata ctttacacag taggtgatta tgtgaaaaaa
    10861 gaaaagaaat atgcaaaaat gccagatggg agcgaaattt actatgaaaa gagcggtcaa
    10921 ggcttccctc tctttttact gcatggcaat gacggcagcg gccgcttttt ttcagaacag
    10981 gtccctgtac tcgaacgcta ctataccgtg tatctagtag atagccgagg acatggtcgt
    11041 tcgacaaacg aagcatctat gttgaatttt cagttaatgg ccgaggatct gaatacgatc
    11101 atgttgctgg aaaaaatcga tcaggcagat tttcttggat ttagtgatgg agcaaatcta
    11161 gcactggtat ttgccagcag cttccctaaa aaagttcatc gattgatttt aaattctgga
    11221 aacacgcttg ttaaaggcgt tcgtttttct gccagagtca tcagtaatat tcattatgca
    11281 tgggtatggt tactctcttt atttcgccca agtcttcgca aaaatctttt agttatcaaa
    11341 cttttgctgc atgacatcgg gcttacagaa aatgatttaa aaaaaatcaa ctcccccact
    11401 ttgatcatcg tgggaaaaaa agatgtaatc aagctaaaac attctttgta tatagctaaa
    11461 actataccaa aagcttcctt tgtgcttgtg aaagaacaag gacatgaatt agctagaaaa
    11521 gatcctgaac gcttcaatcg agaagttctt caatttcttt ctgaaacata actattagga
    11581 atagaaggtg atttatttgt taaaaaatac catacaatgg ctgaaaaacc acctaggact
    11641 gttcaaaaca atctttttga tttctgtcat tgtcattatt gtcggtgaat tgatgtccat
    11701 cggtaaaact ctatctatcc agcaattagg agaaacgttt gccactatac ctgtatggaa
    11761 aagcggacta atgctattga ttggtcttat ctctgtgtta cctatggttg gttatgatat
    11821 tattttgaat cggatgcttg aacaaaagca gaatcctcgt tacctgtttg agacaagctg
    11881 gctgatcaat acgatcaaca atattgccgg tttcggcggg tttgttagtg tggggcttcg
    11941 ctctgaacta tatggacaaa agaaagagag cagaaacgtg atccaagcat tatcgaaagt
    12001 tttcttgttt ttgatggctg gactttctat ttacagtctg ctttcttttt tacttgtagt
    12061 cttcacgcca gttgctcctt ttttgaagca atattggata tggttgatcg gcggcggtct
    12121 atattttcct gtcgtctttt tatttactac tttcaaaaag aaaggattcc ttggtggtac
    12181 ctcacttaaa actcaaggac agctattgct agtttcattg ttggaatgga ccggtgtcct
    12241 gtttagtttc ctttctgttg gttatttaat ggaaatccgg attgatctgt ggcaaacaat
    12301 tccattattc attgctgctt cagtgatagg aatcgtgtct atgattccag gagaaattgg
    12361 gagtttcgat gtaatgatga ttatcgggtt gtccgctatt ggtgtgccaa gagaaatagt
    12421 cgttgtgtgg atcttgctct atcgcctgtt ctactacatc attccgtttt tgattggaat
    12481 cgtctttttc ttcaaaaata tcggttccac ttttgatcag cgttattctg gtatccctaa
    12541 acaattagca acagaaattg cgcataaaat cgtggttgtc ttattatact tttctggaat
    12601 catgctagta ctttctgcca ctatcccgca agcttttaca gagtttcgct ggcttcatag
    12661 tttgaaccca ttgaaatttc attttattat ccaatttcct agtatactat tgggatttct
    12721 tttaatcatt atgggcagag ggatcgcagc acgtgtgaaa cgagcatatt tgcctacaat
    12781 cttcttgatt gttctagctt tactttatgt tttattaagt gattttagtt tcacaccggt
    12841 ggttttcttg tctattctgc ttctgatcat tcttgcttct aaaaacgaat tatttcggga
    12901 acaattgatc tattcatggg agtggcgtac gatcgatgga atcattattg gtgcactgtc
    12961 cttgctttac atcgttattg gggtatacaa tctgccggat ttccctcatc ggcgtcacca
    13021 ttttatttcc tttttcttat ttccttctga aaagatttgg ttttcgggat tgttggccat
    13081 catcgctgtt agtttcatga tcgttctatt tgttcatttc cttcaaggag aaaagaaaca
    13141 gattggcgaa gcctttaatg aagaaaaggc cctgaagatc cttacaactt atggaggaaa
    13201 ctccgatagt caattgatct tcttaaaaga taagcggatg tttgcctatg aaaaagacgg
    13261 tgaaccaact gttcttctcc aattcgcttg cttcaataat aaatgtattg taatgggtga
    13321 tccttctggt aagaaagaag attttccaga agcaatcgaa gcttttatcg aagaaacaga
    13381 tcgactatgc tatttaccag ttttctacga aacaagcgaa gagattgtta tgattttgca
    13441 tgaattcggc tacgatttca ttaagatggg ggaagaagct tatgtagacc tgaatagttt
    13501 tacaacttca gggaaaaaaa tgaagggcac tcgtgctgtc ttgaatcgga tcgaacgaga
    13561 aggatttaca tttgatgtct tacaaccacc attttctact gagcaaatga gtactttcaa
    13621 aaatatctct gataattggt tgggttcacg aaaggaaaaa gggttttctt taggcttttt
    13681 ctctgaagat tatctacaaa gagcgcctat tgcagttgtc aaaaataccc aagaagaaat
    13741 tgtcgctttt gcaacgatca tgcctactta cacgaacaat aaagtaggaa cgattgatct
    13801 gatgcgatat gattctaaaa aagcaccttc tggcagtatg gatttcttgt tcttgcattt
    13861 gtttgcctat atgaaagaag aacatatcca atggttcaat ttagggatgg cccctctttc
    13921 aaatgtcgga acttccagaa agagtttttt acaggaaagg attgcatact tggtttacga
    13981 gtttggttct catttttatt ctttccatgg attaagagaa tataaaagca agtacgctac
    14041 aaactgggtc tcacgttata ctctatattc tagagatagt tggattgcat atgttatgat
    14101 tgctttgctg attattgata atgcgccagt tgagcagaca caaggtagaa taagtggttt
    14161 gaaaaaatgg atacgaagaa agtattaaaa actgcatacc gatagatgaa cactctcatg
    14221 atgaataaaa gagactgaag taaaacgaaa gttcatgctt ctacaatctc ttcttataca
    14281 attgaagaac aaagagcggt ccaaacagaa atcctgtttg gaccgctctt ttattttaaa
    14341 catttttcat taccaaaata ttcgagtcgc gtttcttatg cttcgatctc tgtaacgata
    14401 cctgaaccta ctgtccgtcc gccttcacga attgagaatg tcgttccgtt ttcaacagcg
    14461 attggatgga tcaagtcaac atcaatcgtc acgttgtcac caggcatgac catttctgta
    14521 tcttctggca atgtgattgt tcctgtaaca tcagttgtgc ggaagtagaa ctgtggacga
    14581 tagttgttga agaatggagt atgacgtcca ccttcttctt ttgtcaacac gtacacttct
    14641 gctttgaatt ttgtatgtgg agtaatggaa cctggtttag caagtacttg tccacgttcg
    14701 atatcgtcac gttggatccc ccgtaacaac acgcctacgt tatccccagc ttccccgtaa
    14761 tctaacgttt tacggaacat ttctacacct gttacaactg ctttttgtgt ttcaggtttg
    14821 atccctacga tctctacctc atcaccgaca cgaacagcac cacgatcaat acgacctgag
    14881 gcaacagttc ctcgacctgt aattgagaag acgtcttcca ctggcaatag taatggttta
    14941 tctgtatcac gttctggtgt tgggatatat tcatctactg tatccatcaa ttccataata
    15001 gcagcttcag catctggatc gccttgcaat gcttttaatg cggaaccttt gatcacagga
    15061 gtgtcatcgc ctgggaaacc atattcgctc aataactcgc gaacttccat ttctaccaaa
    15121 tcgatcaatt cttcatcatc gactaaatca actttgttca agaaaacaat caggtatttt
    15181 acaccaactt ggcgtgacaa taaaatgtgt tcacgtgttt gaggcatcgg accgtcagtt
    15241 gcagaaacaa ctaagattgc accatccatt tgtgctgccc ctgtaatcat gtttttcaca
    15301 tagtccgcgt gtcctggtgc atcaatatgc gcgtagtggc gtttttctgt ttcatattct
    15361 acgtgtgcgg tattgatcgt aatcccacgt tcacgttctt ctggagctgc atcaatacta
    15421 gcatagtctt gaggatttgc taggcctttt ttgcctaata ctgtagtgat cgcagcagtt
    15481 aaagtagttt tcccatggtc aacgtgtcca atcgtaccaa tgttaacgtg tggtttactt
    15541 ctgtcataat gttcttttgc cataatttac aaacctccta aataatatag atcactgaaa
    15601 aatttcagct ctcacctttt tattatagtc tttttttgac taaatcaaaa agaatacgtc
    15661 cgacttactt tttgtaagat tcgaactaag cttattatac gtcaatattg gtcaaagtca
    15721 actaagagca ctcgtctgtc ttatacgcga aaaaagtacc ttttataaat aaaaaaatgt
    15781 gttttttgag aaaaacttca agatagtcct taactttctt ttgaagcaat aagaacaaaa
    15841 tgaggaaaag tttaaaattc agctgttgcc aacaactttc aatagacgaa atcttctatt
    15901 tcgttaataa taaggaaaga ggtgaagtac atgaaaaaat taattggcgt acttgtcgtt
    15961 ttaggcatat ttctcggcgg aagttttgca gcttatcatt atttttacgg cggcgaagct
    16021 tattatacaa aaatcacgac gaatggcgaa atgtcagcta cccaagcaga taacggagag
    16081 aaattcacga cttataccta caagcaagcg gcttataata aagatggtga aaaaaaacag
    16141 gtgacgcttc gcgaagaaag agaacatcca ttaaagatga atgcgtatct taaactcaaa
    16201 gtcaatcctc gtaaaggtgt attaagctgg gaagaaataa aggcttctga agtgcctaaa
    16261 aatgcggcag aaaaaatcga taattagtaa atggtcttaa aattttatta gataaaaaga
    16321 ggctgggaca gaagcgttta actccaagaa ataagaagga gttgcttctg cttccgccgt
    16381 ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtctcac gctctttttt
    16441 tgttatattc gtcttttccg atccttctct tgacacaatt catttaactg atcactggta
    16501 cttgaacacc taatattcta gtcattttta taaacgattc aatacttcat ccacattttt
    16561 gatcatcacg gatatcgtac catccggatt attcacaaat tcaatcaaat ctgggttgcg
    16621 atatacatct actggtacga tcagttcgat cccgtttgat agcttgaatt tttgttttcc
    16681 aaattttttc tcagaaattt cttgtacttc ttttacagga ggtgcttctg ggacaaatcc
    16741 ttgctcttgt acttcttctt taaaagccat ttttgccgtg atgttttcct tgaagacttt
    16801 ttccgcaatc atctcatgat ccaaccgacc tttttcttct atcgtatcat agacagcttc
    16861 tttcaaatca gccatgatat caaattcttc tgtctcaaat ttttttccaa gatgcttggc
    16921 tactttttgg atcactttta cattttcttc taaagaaggt gcaggaacac tttcgatcac
    16981 tcttccagaa aaataaagct cttttttgcc agaaaactca tatttttttt caattaattc
    17041 gtaggtccaa tcagtcaaat tcacagcgaa tgcttcatct gctttttgtg ttttcgcccc
    17101 tagaattgct cgattcaaaa tcaagcggtt gtcgatccct gcttcgtctg cttcaacaaa
    17161 gtgagtgtaa gcctcacgat aattgacttt caataaagat aagtacatca ctgtatctaa
    17221 ttcatacaag acaacaaata catctgcact aggtgcttct tcactttctt ggtagatatc
    17281 gaaccaccgc tgaacgattg cttcacttgc ttccacaaaa ttttcttgag cgattttaag
    17341 tgattcaacg atcatgcttt cttctgctag ttgtccagtt tttgtttgag cagaagagat
    17401 tttttggatc ttcttttgaa gaaaatcacg aatatattct tttgtcagat ccagctcttt
    17461 ttgtgaaaag actggagaac cagtttctct gtcaataata tgcagaattg ctttttttaa
    17521 atagatatcc attttctcat tcccttctgt cttagtttac ggaaaaaaaa tcaaaaatac
    17581 cagaatcaaa atcattttct tttaaacaaa aatttcaaga gtagaaacaa aatggattct
    17641 ttttttcgaa aaatgcgcta cacttatagt tgaacgaata aaaaaggagc tgaaagtatg
    17701 aattcattaa caagcactta tcgtctatca aatggttacg aaatcccggt agtaggattc
    17761 ggcacatggc aaacacctga tggggatgtc gctgtttcct ctgttaaaga agcgctggct
    17821 gcaggttatc gccatatcga tactgcccaa ggatacaaga atgaagaaag cgtcggccaa
    17881 gcgattaagg aaagtggaat tcctagagaa gagatattct tgacaacaaa attatggaat
    17941 gcaaaccaca gctatgaact tgtgatgtct tcttttgaag aatcattgaa gaagctccaa
    18001 acagattatt tagatctgtt tttgatccac tggccaaacc ctgttgcttt tcgtgataat
    18061 tgggaacaag caaatgcaga tacatggcga gcatttgaag aactgtatga agcaggtaaa
    18121 atcaaagcaa tcggtgtgag caatttctta ccccaccatc tagatacact agcaaaaaca
    18181 gcaaaaatta tgccgatggt caatcaagtt ttccttgcac caggcgaatt acagacaact
    18241 gtagttgagt atgcaaaaaa acatgatatg atcctcgaag catatagtcc attaggcact
    18301 ggtaaaatct tcgatgtgcc tgaaatgaaa caaatcgctg aagcacatga caagacgatt
    18361 gcacaagttg ctttgcgttg gtcgctgcaa cacgaattcc ttcctcttcc taagtcagtg
    18421 acaccaagca gaatcaaaga aaatactgaa ttatttgatt ttgaattaac agaagaagaa
    18481 atgaaacaaa tcgatcagtt agatggtgta gtcggcaaag caaaagaccc agatactacc
    18541 caattttaag tcgaaaggtg attttatgga tccaatcgaa cgcttgaatt ctctctcgga
    18601 agaagttacc cagacttttc attccgattt tgtctttttg atcgatgcag agaaaatcca
    18661 acattttcct gcaagaaact ggacacatga tcaaatcatc gaagaactga aaaaacggtt
    18721 cgatcattcg ctaatggtca aaccctggca cgaacatgaa gtcatttatt cacctgaact
    18781 acctgttttt gccttgatac cgaaaaaata gaaagaatgg aacg
//
LOCUS       DAXWLG010000041        18783 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXWLG010000041 DAXWLG010000000
VERSION     DAXWLG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.041"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(574..1200)
                     /locus_tag="ACGE4T_001139"
     CDS             complement(574..1200)
                     /locus_tag="ACGE4T_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386432.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1495..1830)
                     /locus_tag="ACGE4T_001140"
     CDS             complement(1495..1830)
                     /locus_tag="ACGE4T_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8386433.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1817..2101)
                     /locus_tag="ACGE4T_001141"
     CDS             complement(1817..2101)
                     /locus_tag="ACGE4T_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFF8386434.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(2157..3680)
                     /locus_tag="ACGE4T_001142"
     CDS             complement(2157..3680)
                     /locus_tag="ACGE4T_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8386435.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3673..4848)
                     /locus_tag="ACGE4T_001143"
     CDS             complement(3673..4848)
                     /locus_tag="ACGE4T_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8386436.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4852..5037)
                     /locus_tag="ACGE4T_001144"
     CDS             complement(4852..5037)
                     /locus_tag="ACGE4T_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386437.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(5003..6697)
                     /locus_tag="ACGE4T_001145"
     CDS             complement(5003..6697)
                     /locus_tag="ACGE4T_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8386438.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6694..7167)
                     /locus_tag="ACGE4T_001146"
     CDS             complement(6694..7167)
                     /locus_tag="ACGE4T_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFF8386439.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7236..7391)
                     /locus_tag="ACGE4T_001147"
     CDS             complement(7236..7391)
                     /locus_tag="ACGE4T_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFF8386440.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7525..7905)
                     /locus_tag="ACGE4T_001148"
     CDS             complement(7525..7905)
                     /locus_tag="ACGE4T_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8386441.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7909..8124)
                     /locus_tag="ACGE4T_001149"
     CDS             complement(7909..8124)
                     /locus_tag="ACGE4T_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386442.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(8127..8534)
                     /locus_tag="ACGE4T_001150"
     CDS             complement(8127..8534)
                     /locus_tag="ACGE4T_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFF8386443.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8800..10275)
                     /locus_tag="ACGE4T_001151"
     CDS             complement(8800..10275)
                     /locus_tag="ACGE4T_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFF8386444.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10265..11113)
                     /locus_tag="ACGE4T_001152"
     CDS             complement(10265..11113)
                     /locus_tag="ACGE4T_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFF8386445.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(11150..11512)
                     /locus_tag="ACGE4T_001153"
     CDS             complement(11150..11512)
                     /locus_tag="ACGE4T_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386446.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11513..11671)
                     /locus_tag="ACGE4T_001154"
     CDS             complement(11513..11671)
                     /locus_tag="ACGE4T_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386447.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11812..12436)
                     /locus_tag="ACGE4T_001155"
                     /pseudo
     CDS             complement(11812..12436)
                     /locus_tag="ACGE4T_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12433..12744)
                     /locus_tag="ACGE4T_001156"
     CDS             complement(12433..12744)
                     /locus_tag="ACGE4T_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386448.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12788..13081)
                     /locus_tag="ACGE4T_001157"
     CDS             complement(12788..13081)
                     /locus_tag="ACGE4T_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386449.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13275..13922
                     /locus_tag="ACGE4T_001158"
     CDS             13275..13922
                     /locus_tag="ACGE4T_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8386450.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13983..15128
                     /locus_tag="ACGE4T_001159"
     CDS             13983..15128
                     /locus_tag="ACGE4T_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8386451.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15567..16385)
                     /locus_tag="ACGE4T_001160"
     CDS             complement(15567..16385)
                     /locus_tag="ACGE4T_001160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386452.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(16485..17810)
                     /locus_tag="ACGE4T_001161"
     CDS             complement(16485..17810)
                     /locus_tag="ACGE4T_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386453.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            complement(17979..>18783)
                     /locus_tag="ACGE4T_001162"
     CDS             complement(17979..>18783)
                     /locus_tag="ACGE4T_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386454.1"
                     /translation="TRHIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQ
                     YYYQINALTINLISSAKTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLL
                     HMRNYSQHGHLPVNIHQQRVYFDLDEILAMPHFDFNGQLKKELDVLRKDIYNQFRDSP
                     RISYVYTVAKFNLVITEIYFNYLKEIKPILIKLNDEKNELLLNTKFQLINSDGSSSDM
                     LFYEFDGENYHCFNRKDNSISMYADIKREVKKILKVEEQYYKEIEKRNQ"
BASE COUNT         5496 a         3761 c         2875 g         6651 t
ORIGIN      
        1 acagccaaac aaaaatagct cctcataatt ttcgtttggt tgaacttatt accgcagatg
       61 caagcttttg aacaccgttt aagaaagagg tcgtgggcct gccgttcagc tccgagtcac
      121 aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgag
      181 gagttggcag gagaacaccg tttaaggaag agactgtgaa gtcaacgttt tgacctgatt
      241 attagagaaa caaatggcaa aatagttctt catatttcgc cgtttttcga gcgaatgccg
      301 aggggcagct gattgaacat cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca
      361 caatcccttt ttttatatct tattaattca ttcttttgtt ctattcttct tcttccgcca
      421 tgaatgagaa acccatatca aagaactata aaggttatca aaacagcgtt tatgactaaa
      481 tgaattagag ttatctatca ctatttttca agcgatttaa accaaatggc atacaatatg
      541 gcatacaaaa acttcaataa gccgtggggg tttttaaatt tcttcttgtt ctttaaaatc
      601 aatgagtgga gctacataat ttgaatccac tttttcaaac gtcacttttt gcatcgacga
      661 atgttttctt tcccaagata acgtaaatag atttgactct ggacttataa agtctacttc
      721 accttctagt attactttct tatgcaaata ttcctctaat aaatatagac ttgctaaagc
      781 atacaacaca ttttccaaat ttgcttcctt ataatttgaa gaacgaccat gttttactct
      841 gttatatatt ttccaccaat ctggtgtatc ttcaggccac ccatctccaa aaggttttag
      901 tgaagtcaat gttaaagaat tcataaccct aacaccatta tccaaacaca ttaattcgct
      961 agatttaata acagctttaa aatcagttat gcttttattg ctactgctat caaatccgca
     1021 aagttctttg aaaataatct cacactccga accaatcgct agtagtagtt tttgatattc
     1081 aatagagaat gttttgaaat tattctcatc gagcatcaca aaatctgtgg tattaataaa
     1141 ctcattttct aaatgtagat aatacttcca ataaaaattt gtaaactctg ctttgtccat
     1201 taaatcacct cttttgaatt ataacaaaaa aaacacgtcc acaacagacg tgttctttga
     1261 tttctacaca atcagcttgc caaaaccatc caatcagtcc tagcatgcct gtattaaaat
     1321 tgaacgttca tcatctatca tctttcttag tccagcatgt ttgcaaccac acctacaatt
     1381 ttcagtccaa caatagattt cgactagcta agtcttgcaa aattcaggca gctaaccctt
     1441 catttactac cttaattata ctaaataccc acaacgttta caagtgtaat cattctaaga
     1501 aatctgttta gctatgatcg ttgtataatc tttaaacgcc gttccttcat tgattgaaat
     1561 aattttataa ctcttgtttt tccaagctac tttcatatcg tttgttagtt cataatcttg
     1621 atcgtatcga ataataaacg tcaacgttcc ttcaagaatg gtacctacac tagcaagcac
     1681 gtcttttagc atttgtgaac gcacattagc ccaacattca aatacagttt cttcactatc
     1741 caccatctgc ccatcttcat cttttacccg ttttgtcttt ttaaacttga ctctctcagt
     1801 caattcattt acattcttaa tgattggcat aataagcacc tctcatttgt tgtactaatg
     1861 cttgtaccgt tactggtatc cgttcgctgc tggcttcttg acggttcaaa taccaatgtt
     1921 gagtaagtaa tgacaccgcc aaatcaaatt gctgataatt atccatatct ttatccgttg
     1981 cgctactatc aatagcactt tttatatatt cgcctgctgt atcaatcaat tgttgaataa
     2041 agtcatcatc aatagtgtga tcgatccgca tacttttttt tatgctatcc aaaattacca
     2101 ttttatctct cctaatgaaa aaaaggcagt gggataaacc gccgcccaat atttttctat
     2161 acttttggtg ttacaactgg cgtaaactca atatacactg aagcattttg atcaattttc
     2221 ttataatcgt tgcgaacgat cactgcaagt ccttgtgagt agtaatcgaa tttttcccat
     2281 tgagtcgtta cttgatttct acgagcaaca aagatagatt gtgcaatatc ccccataatc
     2341 atagggaatg ttccagcttt aggattggca aacaatgtat ctgcaatcaa tactactggc
     2401 ataccgaata atgatttacc actaggtgct gtcacatcgg gttgtaaaat gtaacgtcca
     2461 tctgaatctt tcaatgtatc taggtggtaa tatccgcttt ggtttagaat taccatttta
     2521 tttaatgctg ggtctaatgc cacattgtat agttgtttca aatcgtccaa cgtagcagcc
     2581 gttttcttag tgaatgtttt taataaatcc atgatatgct tattgtcagt gttttctacc
     2641 aatttcgcta actgatcttt gacctcttgt acaatattca ctgctgaatc ttccacaacc
     2701 tcatttgata aggcaatctt accagcacga gtttctactt tataatcaac tgaagtaaac
     2761 atttcagcgt caatatcacc aatttcagca agttcagctt ttgttgctaa cactgcttgt
     2821 tggttagttg ctactggata tttaccttgt ccatttgata ctgtttttac tgttgcatat
     2881 tgagctaagt tataatttga acgtttcaaa tcaaagactt caccgattac ttcttcggga
     2941 acaactgccg ctgcatttac agtagtgact ccatcacgca cttcgccttg tgaacggata
     3001 tattcttcat acccacgcaa ttctgttttt tcgttatcga ttaaagtttt ttccatgtta
     3061 ttactctcct tttgtgcttt agaagtggat tctaagctat tgtttgtttg ttttgcttgg
     3121 ttgttactca taaacgattc gtaagaacgc ttatttactt ggacatttga tgaatcataa
     3181 gcgggaacgg tcacgacact aatttcatta agtgctttga ttttatctat tgatcgttca
     3241 attgtgccat cttctttttt gtcgaatgaa tctaatccaa gcacgaaccc aaaggacata
     3301 gaatcaacaa ccccttttga gatattctca tacacatcat ttgaataggt cgtgtcggtt
     3361 agctctgctt caaaatgtaa tcctgtatca tcgacattca atttcaacgt acctgctttg
     3421 acactagcta gtggctttga ataatcgtgt ccatataata agaacacatt tgataaatcc
     3481 acctctttta atgcttcggg tgtaatcact tctacaaagc cgcctaaatc ttttgacggt
     3541 tgcccgaatt tcaacgcata gcctgaaata gttttgccgt ctgttggttg tttttcattt
     3601 tctttctcta ctttgggaga atcggctgtt agctcagctt cttctgtcag ccgtttttcc
     3661 ttttcttctt cattattcaa ctggtgtaac tccttcctgt tcttttaatt gatatttctc
     3721 caacgtatct aaatacgtaa agttcaaact tgctagtaaa cgatcgccac catccatggg
     3781 gggtagcccc attttcgacc gcccctcatt aatcgtgaga agtgaccctt tgacctgatc
     3841 caatacattt tttacttttg tttcaggatc agtctctagt aactgatcgg aattaaagcg
     3901 gatattggtt tcaagtttcg tatctaattc actcacaaac acattaaagt aatgaatcag
     3961 cgtactttgg atatactgga gattactttg aacggtgcta gaatgttcat tttcaacgcc
     4021 taaacgctca atcggtacac caaacgcctt ggcaatttgt tttgtgttcc aatcattgga
     4081 attgataagc ttcaaaacat cggtgtttat ttctaatgtt ttgtagtcca ttgtttcatc
     4141 aagaatgatc gttctaagag cattatctcc actacttgaa ccgttggctt cttcaaactt
     4201 ttcacgaatt gcgttctttg ctgatccgtc taaatctgac ttatgaacct tcaaaatacc
     4261 tgacccactg acaccacgag taaagaaatt gtgcaatgtg cggttgccag cttgttgaat
     4321 tttcagttca tcacgtaacg cataaagtgg tggtaatcct gttaaaccat cttgggtgaa
     4381 atatttgaag tgcaaaatat cgctagactt cacacgtctt tttttatcac caatctgata
     4441 agacaacgta ccattatcta attgagtaac cgttacactt gagttaggta ataagtgaat
     4501 ttcttctacc ttttcaccgt tgcgtttaat ctctgcaaag ctattaccgt ttagcaacat
     4561 attgaccgcc aaagcaaact ttaaatgcca accatccatt tctgaattag gtttctcgtt
     4621 tagtaacttc accagttcat tatcagcttg tggcatattg tttttgacca attgaatcgg
     4681 acttgaagca atatcactag caataattcg aacggctgca aacacatcac tatttcttaa
     4741 tgcacgaacg cttgtaaagc tggtggtgta atcatccgat tggattgata ccacatgttg
     4801 taggaaagga tctcccgttg tattttgtct cgctttaaaa aatgccatct tttaacctcc
     4861 cttctgttct gtatttagca tgaccgctac cccaataagc actatgccca gcgctaacaa
     4921 gccatagtag acatttgtta gtaaggtgat agcaaccaac acacacacca atccaagaag
     4981 taaaagaacg gtatgaatgt aattagaaag aaaattcttc acttgtataa aattcattgt
     5041 ctgctttcgc tccttccgtt gtggtgaagt aatccattgc aaacacatag gcattgatta
     5101 atgcagctat tggatcaatt ttgttactgt tctttgattt attaatctga atgccgttgt
     5161 tatccgtttt taaaatagcg ttgttcactg catgggtgag aatcgtattt ttattgtgaa
     5221 taacgttgcc ttcataaact tgttctctaa aagttcgggt cggaacgttt agagtaatcg
     5281 ttccttgtct tacttctaac attgggtagt tggctttttc agctttactg ataagcgaat
     5341 tagcgttata cgggtcataa caaatcccca tacattccaa atcattttct tgaatcaaat
     5401 cttgaataaa ctgaaatact tggtcatagt ccacgattcc actttctaat tgagtaatgg
     5461 aacactcacc agctttttct agttctcgat aaggcaaacc gtcgcgcttt tctttgtctt
     5521 gaagcccata tttggtggct acaaagctat gtgaatcaca ataaagttcg ccgttatcta
     5581 gcggtacgat ccatgaaacg ctagttaaat catctgtttt agataaatcc actccgatat
     5641 aaacgggctt gcctgtaata tcttgtggtt ctacttcggt tgcctgccag tcttcagcaa
     5701 tcatatagct gtcctcactc gcttgtctcc ataaattgaa gtttttcacc aatacagaat
     5761 taaggttatt ttgtttcaaa gcaaggttca catcatcttg aatggttgga atcattactt
     5821 ttttgatttc ttcactttca aaaatcggat tggctttgat ccatttctct tgatcgtgaa
     5881 tttcttcggg atcgtccaat tcccatatcg ctataaagta cctgtcggct tgttcttcgc
     5941 cttttagcac acgatctaac aataagtatt cctcgtacat aggaacgttt aaatcaagcc
     6001 cagcggtact aataacacac aataagccgt tcttttgttg ggtcattcct gacttaatga
     6061 cgttgtacgt tttacgagtt tttgcttcgt gccactcgtc aagaattgcg gttgttggtg
     6121 caaacccatc tagcgtgctg gtttcactag ccagtgccat agcgaacgaa ttagagggca
     6181 agtgctgaat cttagagttc ataattttca gttgtggtct taaaaactta ctagacttga
     6241 ccacgtttct aagtgaatta gacagcatat catagcccaa tttagcttgt ttcaaagcgt
     6301 tacttacaaa caatacttgc cttgcttctg ctggttcttg ttccataatc aacgcattgg
     6361 cagccatgcc acttgcgata tacgttttcc cattcttacg gctcatactg gtaaacgctc
     6421 gattgaatcg cctgtaattc ccttctttag tacgccagcc ataaagactg cctagaatga
     6481 atttttgaaa tcctaacatc ttgattgctt taccgtccgt agatggtagc aactcaatga
     6541 atttaatcgc tttggtggct tgttcttcat cgaacacata agggaagcta tctgacttag
     6601 aacgctccaa atcgcttata tgacgctcac acgcttggat aattttcttg ggtgcaatca
     6661 gcttacctga taggactttc tcaatgtaat cattcatgaa ttcatcaact ctttgaacgg
     6721 gtcttcgggt tcatcatttt taaccttgtt caaagctagt tttgatcgtg attctaaggt
     6781 catacctagt gaggtggctt gttttttcaa atcactcata gcttgtgatt gaacccgaac
     6841 cgctggattg gctttcttta ctccgttttc gcatgtgata aacgtacctg attttcttat
     6901 ctcggcttcg gctgttttca ttcgagcaaa ggctaaacaa taggctacca atgaaccata
     6961 atctgcttca ctcaatggat aatctttttt taatgttggt acgatacgat tccattcact
     7021 tctagcgctc gctgccatcc agtcaggggg cgtgatattt attaatggtt gctgcttgaa
     7081 caattcttct ctagcgtcca cacggattgc tttttcttca cttgatatat tgccttttgt
     7141 atcgtgtaat attttagggt gtcccatgtt gtcctccttt cagacaaaag aaaaaggaac
     7201 gtaagcaatt aagctcacat tccttcgttt ttcgatcaga atttttcttt atctaattta
     7261 tctatataaa tgatttttcc gttctgcatt tgaatggtca cttgaccaaa atgaggtaac
     7321 tcaatggtgc gctgtttccc tgttttatca gtaataacaa ttgtattttt caataaatct
     7381 cctactttca ttatatactt tttcacttgt gattacaagt gtaatcgtta tttgtaatta
     7441 cccttatgcc ttttcacaac ggttatttgt accaaaatga gtaggcatcg attgtcagta
     7501 agtttcacac tatgccccct taactcaaag ggtactcttt tctcgtcttt gtctttcatt
     7561 ggctgtcttg gaattatggt gtgcatggca taaagaacga aaattagttt catctaaccg
     7621 tctattccaa tcatctttta gttcaatttt gtgatctaca acatcagctt tccttatcaa
     7681 gccttcttct aggcaatcct cacatactgg atgatttaac cgatacaagt atgaagcctt
     7741 tcgccatgtt ctactttgat agaattgaaa atatttacct tcgctttgct ttctttcttc
     7801 acgcttaatt gccgtttgct ttgctttatg cttcccacaa tacttctgcc tataatcaat
     7861 caatgtttta catccagcat gattacattg ttttttaact gccatagatt accaaccaaa
     7921 gcgatctcgt tcgtcagaat catcttgttc ccattttgat ttgtattcaa tataaggcga
     7981 aactatcttc aagcacgttc cgtctttaaa attaacttta gtgattttat cttcctcaaa
     8041 actaataaag tctacatctt cttgagtgtt tgaatactca atgtgtttct caccatcgaa
     8101 gtatctaata ctttccatat gcataattat tctacctcca ataaattagt tatcaattca
     8161 tcatctgtta gtagcaaagc caagaactta tcatggatgt ttaaaaacaa attatcctca
     8221 ttcgatgtaa gctctcgttg tctcgctcga tcatataggt taagcagaca attaaattca
     8281 tccattagct cacctgtatc atcatagttg ccactatcta ccaatgatcc cactactagc
     8341 gattgacacc acgctactga ctcctgcagc caatcaggtt cttcttccat tatctttaat
     8401 acttctggat caaatgtgga ataatcttct gttaccaatc ccacactaaa tgccagcgtg
     8461 ttcattagaa accgatccat ttcatctgta tattgaaagt tcggtgcttt gtttgttgaa
     8521 ataatattga gcatttactt tcctccttga ttcgtaacac gtggtaacac ctacgtaaca
     8581 ggtacccgtt accactcaat cccaacgggg acaagggttc tagcgttttg gtaacaggta
     8641 acgggtaaaa tccagtaaca ttattcccca gcatattagc taactatttc aaaagtttta
     8701 aggaaataag gtgttacccc gttaccaaat tgctgtatcc cttggggcag taagggtaaa
     8761 ctagtaacgg gctaggtgtt actgacccgt tactacccgt tactttttgt actttccagc
     8821 tcgattttta ttttcatagg catttgactt attgtaaaat cctcgtcgtt tatttctttt
     8881 tggcagttgc ttccgttccc aaccaggaat actgctaata atcaaggcaa tctttttagt
     8941 ttctgcatct gatcgaccat tcaataaatc ctttgcttgt ttatcaaaaa cagcttccaa
     9001 aatatcagcg gtgagtacgc tatcaactaa ataaaactct tgttgcatag tagctccatt
     9061 atagaaatgc tgtaaatttt tctcattaca tttactgtca ggatagttct gataataaga
     9121 acgtctcgcc cacgctggag cttcttccca tttatacggg atttccattt ccaaatattt
     9181 agtgattgct tctttaattg gattctccat cttggcatcc tcttgatagt cttttgcaat
     9241 ttctaagact tctttatctt catcgggact aaaatagatt cgttgtcctt tatcaaaaag
     9301 cacctttgct tcagctaaca cttgaagaaa atattcatcc tttgttttaa acggattcat
     9361 ctttggctta ttcttacaag gcaaaggata aaaacggcgg tttcctgtat catctttcaa
     9421 atactgccca tcattcgacg ttccaataaa tacacattgc cttgcccaag ctatgacatt
     9481 ccgaccataa ggaggtctaa ttttatcgat cttggcactg atgaagtttt ttatctgttc
     9541 aattttagtt ttgttcattg aagataattc tccaagttca ataattacat tgccttgaag
     9601 ctgcatataa tcatctttct tttcacctag cgaatctaat gaatcggtga aaaaatcatc
     9661 cgtgtaaagt aatgctggaa cggtactctt accaatccct tgcggtccat ccaaaactgg
     9721 aacgatctca aactttattc ctggtttata gatacgtgcg actgatccga ctatccaacg
     9781 ttttgttact tctcgtgtgt aaaggttgtc gtctacccct aaaaggtcaa taaacagtgt
     9841 ttccagtcta ggctgttgat cccatttaac tttttcgata cgttctttta ctgggttgaa
     9901 aacattcttt ctagccacat ttactactgc tttgtgtatt ttgttgtcat caaaaactac
     9961 attgtagttt tcttcaatat atgccgttaa ggcatcctca aaactgtctt cccattctcc
    10021 agcggtacga ttgatccaag gagaatcttt taataggtga atgtatccgc taaattcgtt
    10081 ataagcaatt gttccagcta aattgctatc attggttaaa atcaattctg cattcacaac
    10141 actgttactt ttaatctgat cgtctttatt gcatcttaat cgtgacatcc aggttttcaa
    10201 ttcaatgata ttgtcttcac gctcgctggc ttcgggaaag tctaaaatac tatctaattt
    10261 atgcttatga gccatgagct ttccccctca aatctttttt aatcatcgat cgaataattt
    10321 gattcacttc tttatctttt agcggtggtt cattgtacgc atttgctgta taaatcaatt
    10381 ctgctaaagt ttgattatct acaccgtgcc atagtaacca accgcataac ttagttaaat
    10441 gatcgtttct actaccttcg ccagcgcctt gaacaatcat atctaatttt tttgctgtcc
    10501 ctgtttttgc tacgggtcgg tattgagtct ttatttcttg cggttttttt agcagtaggt
    10561 tcactagcca atcgggagct ggtgcaatat tatttagaga tacgttttca ataggttcat
    10621 atgctctccc aacacttctt gaaataatca tagttggcgc tattggaact cctttagtga
    10681 taatatcaat cccgcttaaa cttttacctt tatcatcacg cccgaagata tcagcaaatg
    10741 cacctgtttc ttgttggata attacaccat caggaatttt aaagaagaaa tggattcctt
    10801 tacctggtgt gatttccgta taagtagaag gaaatggatc atacattgaa gcaagttttt
    10861 tataattctc aacgccgttt cgtgtttcag aatgttgatc taggtcaact accaccattt
    10921 gatgatcttc aagcattaac ccaatatttc tagttgcatc ttccgtccac cattcattaa
    10981 tttgattaga atcgttagtt gcatcattcc aaccactaga gccttttatt ggaattttag
    11041 ttccaggaga taagggataa attgaccatc ccgacttttg taattccgtt gctgcttgat
    11101 aattatttgc caataaacct ctcctccttt ttcaaaatac tggtataaat cattcagaca
    11161 cctccatgag cttcaactgc tcaagttcgt atttcaaccc atcccgaaca aattcagcac
    11221 cttttaattg attctgatac tttacccatg tattaaatgt tttcaccctt gcttcttgca
    11281 tatcagtaat gtttaaccat gcggttaaaa tctgattttt tagctcgttg atttctcgac
    11341 aaattatttt gtgttctgca tgttttttga gtacatacaa aactgttagt ttttcattga
    11401 atgaatttcc aaagtaggtt ttcccgatat tattttctag ttcagcgatc ccttgattaa
    11461 tttcctctaa ttcaaggcta tcgagttggt ttaatagttt tgttttgtac atctacttat
    11521 gccctccagt tcttaattga tagtcgtact cgtcataagc agtgaagaga tataacgcta
    11581 atcctgcaaa gatagctatt tttgcaatta atggtaataa gccgaaaatt ccgccgatta
    11641 aaatgcttcc tataattaag tttcgtttca taatggttcc ctccgatttt ttaatatggt
    11701 ataatcggaa caaagaatat cgggttttat cccaattaag ttgcctttat cctgtccgcc
    11761 aagactgtta tggataaggc tttttctttg ttctcgatca tgttaatgct cctaagttgt
    11821 ctgcaattca gcagaacgat tataaatttt ctcactcact gaaaactgca acccatattt
    11881 ttctttaaca gtcattaatt ctactgtctc atctaaaata ggttcacgat caaccaacat
    11941 ttcaggagtc atatctttct tttttaccat tttggaatga ccataaatag ttgatacagc
    12001 tttattggct atcacattcg ctttaatgta gtctactggt tttggttcag taatagcgtg
    12061 gcttaattta gtcattgctt ctttttggtg ttctttgtct agcatgcgga atacttgaaa
    12121 gccttcaagt cctgtggact ggcgtagttc tttgatgatg tcgaaaatcc agtcttgaaa
    12181 tgcctcggct tcttttttac ggcttttgaa tgccatgcgg taaatatttt ttttcgttga
    12241 tgatgtttac atcttgtgtt ctgcccaatg aatcgatgac cttactagta gtaaccccat
    12301 ctttaggcaa agagtgaatg gctctagtaa cctgtttaag acctaacgct tccgcaacat
    12361 ctttcgccac tgcccaccat tcatcattaa tatcgacaaa tcttataatg tgtccgttcc
    12421 aaatttcagt tttcattcaa cgtcctccag ttcaagccct agcaagctcg ctaacaactc
    12481 aacttgctga tacaaaattt cttgatactc ttcaatagaa agtcgttctt taaactctgc
    12541 ttgtactaaa tcgttcaagc cacgcaatac tccgcttacc gttggttctt gtggtagcaa
    12601 tttttgatag tccatttgtt cgtacaaaca ttcaaacgtt tcttctagcg ctttgccttc
    12661 caagtttgct aattctgccg ctgtgtcttc tgtaagaatt gttgataata ctttttcgat
    12721 tgctttattt tgtgtcgatt ccattattat ttcctcctat taatcccttt gcccccacgg
    12781 cttgcgttta tttttggtat tcttccataa agcgatcaca ttcatcttgt gaaattctta
    12841 cgactccatc aactataatc aactttaaac cgttaggaac atatttctgc atcatagtat
    12901 tgtatgaaac gttcatatac ttagcagctt gtgccttgtt catatatttt ggaaactggt
    12961 actttttttc taataatgaa agtaacgttc cttgagtttg ctctacttgt tgaacaactc
    13021 gatcgtcaat aattttaaaa gctggatcat caagcgtgag tttgtcaatt tttaattcca
    13081 tatttatccc ccctcgttag catacatttc tatacaaaga ttttaaaaaa atgcaagagg
    13141 ctcttgaata catttctata cctcttataa actcaatata ccatcatacc ttttcatttg
    13201 caagaatttt aacatacttt tttgaacttt tatgtttcac acatatttaa atcgtgatat
    13261 agtagagaaa atatatgaat ggtggtgaaa atgtggctag aaaaaaatac cccgaagcag
    13321 tggagacagg acaaagaatt agactggcta gaattgaaaa gggactctca atggaacaac
    13381 ttggaggtaa attatcccca ccggccagta aaggtgccgt tagtaattgg gaaaatggct
    13441 ataatcttcc caataacacc aggcttaaac aactttcaaa ggtattagat gtatccacta
    13501 cctatctatt aaacggatct tatacattaa gagatatgca tatgatgccc gaaattaagc
    13561 gaaatgtgct tgaaaagcaa ttgaaaaaaa ctattgaaaa aaataactct ataattgatt
    13621 ctaatctttc taatgctatc aataacatcg aagaagataa cctgactgtc tcacagaaga
    13681 tttatttaac aaacgcaata aaatatctta aaacaggatc tcgaattgaa tttcctgacg
    13741 ggagtacccc aattgcttat ctcgcagctc ttctaaatac gcttttacga tatgactcat
    13801 taattaatga ttcagaaaaa aatacagatg agaaaaaaga gtatcaagaa ttttatgaaa
    13861 atgatcttct aaaagagttc acagctcttg ttactgagct aaataaacac tattcaaaat
    13921 aataataaac tgcctcctcc aaccgccccc acggcttgct acactatgga gggaaaacta
    13981 ttatggcaac attcaagcaa tatgaaacta aaaaaggtaa attttggctc tatgaagcct
    14041 atcttggaat gaacaaaatg actggcaaac ctgataaggt tcgtagacga ggttacaaaa
    14101 caaaaaaaga agcccagctt gccttgtcac gcttgcaagt ggactacgat aaaaacggta
    14161 ttaaaaaagc gaacaacgaa acgtttaaag aagtttatga cttgtggcta gaaacatata
    14221 aaaccactgt ccgagaagcc accttcatga aaactgaaat caagtttaga aagtggattc
    14281 ttccaaaata tggaaatctt cgtgtgaatg aagtcacggt aaaaaaagcc caacaaattg
    14341 taaatagctg ggctgaaact actgaccaat acaaggtact tcattcaact tctaagcgta
    14401 tttttagcta cgctatcaat cttgggatta ttgagactaa tccactagac aacgtattaa
    14461 tgcctaaaag aaccaccact aagcataaag aaaaggtcaa agtttacagt aaagaacaac
    14521 tgatcacctt gtttgattac ctaaacagca aacctgaaac ttatcgaaac gactttgata
    14581 aaacgttact acgctttctc ttttatagcg gggttaggat cagtgaagcc cttgcattga
    14641 attggtcaga cattgatttt caagaaaata cggtcaccat caataaaaca ctaagccaat
    14701 ccaaaaatgg ctataaaatc agtgatccta aaactgattg tagtgctggg gttcttccac
    14761 tagatgaaag aacaatttct atattaaaaa aatggcagat taatcagcgg aaatatatgc
    14821 tgacacttgg tgtaactgat ccgactatga ttttttgtgg tatctataaa gaactaatca
    14881 cacaccacgc tatttatgca agattgatta ctatttgtga aaaagctatg gttccatttc
    14941 tcggtgtgca tgtagcaaga cacactcatg cttcaatgct cttggacgct ggagctacta
    15001 tggtagaagt tcaaactaga ttacggcata caaaaattag tcagacgatc gattgttacg
    15061 gtcacttggc aaaagaaacg aaagaaaaga ctgttgaaaa attagtacaa cacttaaata
    15121 ttgactagat ggcatacaat atggcataca gtaaaaaata aaaaaaggag aaaccctatc
    15181 atatcaaggt ttctcctagc tttatagtct attcttcttc ttccgccatg aagttaagct
    15241 cattaaagat aattgacgat agttggtatt tcaacgattt ttgaatagaa ctaatcactt
    15301 tattaagtct aattcacctt aagtggtttt ttagtcgtat tcaaaaacgt attcaagttg
    15361 cgtggcgtgc ttttgataag aaatcgtaag aactagttaa tcaaacgact tataaataat
    15421 gtaacaccac tgattaatca gctatatcca aaaaaacaag cattcattca gatacatcaa
    15481 tctctataga atacaaagct ttcattatct caaaccatta ccacaacttg gcaagtcggt
    15541 taattgaaga acacctcatt ctccacttat agcagtggag caacaagttg agctattgtt
    15601 gcaacagaag ctaagaattc tgtactccat ttaagttttt ttagactatc taccgcaaac
    15661 ctcagcatag tttttttagg cttttcttta atagcttcat ctttaatggt ttcgatatac
    15721 tcctgagcct cttctacctc ttcttcaggt aaggaatcat tcaaattctc caaaagcaaa
    15781 tcaagctttg ataataattc cgacatatca tcaatagaaa caatcatatt attattgtgg
    15841 ccttgctgta tattagtgtt gtaactattt ccaaatatat ttactttatt atatattgaa
    15901 ttgttgctca tatagtcctc cagtcgtcct gctgcattac ttgcatcttg taacgtcaca
    15961 aaaaaatctc tcccatgttg gtaaattgat tcaacgattt caaattctgg atttccatat
    16021 tcgcgccatt cgtattcttc cttaaagtat tttccgtaac tatggttaaa ttgcacagaa
    16081 tttttaccaa ttttccttag aagttttatc tctctctcag aaataaaatt ttcctcaaaa
    16141 gctgattcaa ctatctgaaa atcactaaga aatgttccta aacagaagat tgattcatta
    16201 atagtttgtt gaaaattcca tggtattttg ttccaaaaac cattcttaat taaagcctct
    16261 tttttttcaa caatctgtga ttttacattt tctgtatata tcttccaatt attgacgtct
    16321 tccttaatgt ccgaaatcga ttgaccttca taagaagtcc ctccaccaaa aagtccttga
    16381 accattttat ctcatcctct ctataataat tactctaaat ccaatacttg ctacaaatga
    16441 tagcatttcg tgtggtaaca tgattgccat cgttcctgtt ttatttactc tgacttcaat
    16501 acttggtttg ttgatgaaat tttaccaatt gattccatcg atcgtttcat ggaatctgta
    16561 tttactattt tcattgattg tagagattga tttattccct ctgttgcact tctgatacct
    16621 ttagtatcaa agttttgcat gattgctcgc tgagctgata aagctgataa tgtttgacct
    16681 attgactcta tcgacctctt catggaatct gtatttaata tttttattga ttgtagagat
    16741 tgatttattc cctctgttgc ccttctgata ccttcagtat caaagttctg cataatttct
    16801 tgctggactg ataatgtttg acctattgac tccatcgact ttttcatgga atctgtattt
    16861 aatactttca ttgctgatag agactctgac aatttagtaa cacctgctaa atcgatatta
    16921 ctaaaataat ttccaatata tccttcaaaa tcatctgaca tatcactaag gtcctcacca
    16981 gcaatatctc cagccaatat tttttgaaaa gctttctcta gttgatttgt atatctcttc
    17041 aatttcttta ttaatttttg gtataaagcc atttctattt ctttgttatg gcttacaata
    17101 ttcctacctt ctctgattaa actcatgtct gtttgaattt cttctagctt aacatccata
    17161 tatttactaa atacttcact ccacaaagaa tatgaatcag atatagtgtg actgttatct
    17221 tgaaataact tcaataattg atcctgagat atttctttat accttacaat attctcttca
    17281 ttttcttgtg taactaaaat ataattttct ccaaagagat attcttcaaa atcatataaa
    17341 tctatccatt gtaaaagatt ctttgagtca aactttccta ttgcttgtat ttgttttctt
    17401 tttattgacg aaatgatttc atttggaatt gattcctctg cccagttatc agaaaaatat
    17461 gccggagcta ataaatagat taattttctt aatgcccatt cgtaactatg caaatacggt
    17521 aatagttttt tcgaaaaaaa caacgcattt gtatctaaaa gggtagtgta gtttaatttt
    17581 gatttacttg cttcaatatt gaccttagct aggaaaattt gaaatatatg tacctctttt
    17641 attcctatct taccagtctt attatattga aatactacgt gtaacgaatc attatagttc
    17701 tttatgtcaa agccaaattt ataatttttt tcctgctcct gaaatcttaa gatagaatca
    17761 ctaatatatg gtgacaacac tttcagaaag gtttcttcga tattttccat tcttcctttt
    17821 ttaggataat ataaatattc aacaacttgc ttactaatat gtcatcaact tctttccttt
    17881 caacaatttt aattttccta ttaacttgat atttgctaca taagccacaa ctagcaaaca
    17941 gaaagagaac tttttcttta ccttgataac tgtgttaatt actggtttct tttttctatt
    18001 tctttatagt attgttcttc tacttttaat atttttttaa cttctctttt tatgtcagca
    18061 tacatagata tactgttgtc ctttcggttg aagcagtgat aattctcacc atcaaactca
    18121 taaaaaagca tatcacttga gcttccatct gaattaatta actgaaattt ggtattgagt
    18181 agtaattcat ttttctcgtc attcaacttt attaaaatag gtttgatctc ttttaaataa
    18241 ttgaaatata tttcggttat tactaaatta aatttcgcta ctgtataaac ataagagata
    18301 cgtggggaat ctctaaattg gttatatata tctttcctta aaacatctaa ctcctttttt
    18361 aattgtccgt taaaatcaaa atgaggcatt gctagaattt catccaaatc aaagtacact
    18421 ctttgttgat gaatatttac tggcagatga ccatgctgag aataatttct catatgtaat
    18481 aaaaacctat acgaaaaatg ctcatcataa ggcttactca gaattttatt cttaaaagat
    18541 ttaaaatctt tttctgctaa aaagtttttc atacaaactt caatggattc agtcaaagtt
    18601 ttggctgaac taattaagtt tatcgtcaaa gcatttatct gataataata ctggtcttcc
    18661 gttaattttt cacctgtact ttcaattaaa tcattagtaa aaattgtgaa gtgtttgagc
    18721 aaattagtta aattatatct aaacacatta aaaagtttat ccaactctat aatatgacgt
    18781 gtt
//
LOCUS       DAXWLG010000042        18727 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXWLG010000042 DAXWLG010000000
VERSION     DAXWLG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.042"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            86..1770
                     /locus_tag="ACGE4T_001163"
                     /pseudo
     CDS             86..1770
                     /locus_tag="ACGE4T_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            1784..3067
                     /locus_tag="ACGE4T_001164"
     CDS             1784..3067
                     /locus_tag="ACGE4T_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8386455.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3137..4006
                     /locus_tag="ACGE4T_001165"
     CDS             3137..4006
                     /locus_tag="ACGE4T_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386456.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4003..4839
                     /locus_tag="ACGE4T_001166"
     CDS             4003..4839
                     /locus_tag="ACGE4T_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386457.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4865..5932
                     /locus_tag="ACGE4T_001167"
     CDS             4865..5932
                     /locus_tag="ACGE4T_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFF8386458.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5929..6756
                     /locus_tag="ACGE4T_001168"
     CDS             5929..6756
                     /locus_tag="ACGE4T_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8386459.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6801..7826
                     /locus_tag="ACGE4T_001169"
     CDS             6801..7826
                     /locus_tag="ACGE4T_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8386460.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7823..10006
                     /locus_tag="ACGE4T_001170"
     CDS             7823..10006
                     /locus_tag="ACGE4T_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFF8386461.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10006..11134
                     /locus_tag="ACGE4T_001171"
                     /pseudo
     CDS             10006..11134
                     /locus_tag="ACGE4T_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            11131..11808
                     /gene="pgmB"
                     /locus_tag="ACGE4T_001172"
     CDS             11131..11808
                     /gene="pgmB"
                     /locus_tag="ACGE4T_001172"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8386462.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11892..12419
                     /gene="msrA"
                     /locus_tag="ACGE4T_001173"
     CDS             11892..12419
                     /gene="msrA"
                     /locus_tag="ACGE4T_001173"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8386463.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12620..13306)
                     /locus_tag="ACGE4T_001174"
     CDS             complement(12620..13306)
                     /locus_tag="ACGE4T_001174"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFF8386464.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13386..14924
                     /locus_tag="ACGE4T_001175"
     CDS             13386..14924
                     /locus_tag="ACGE4T_001175"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFF8386465.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15044..15511
                     /locus_tag="ACGE4T_001176"
     CDS             15044..15511
                     /locus_tag="ACGE4T_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFF8386466.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16045..16473
                     /gene="rplM"
                     /locus_tag="ACGE4T_001177"
     CDS             16045..16473
                     /gene="rplM"
                     /locus_tag="ACGE4T_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFF8386467.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16489..16881
                     /gene="rpsI"
                     /locus_tag="ACGE4T_001178"
     CDS             16489..16881
                     /gene="rpsI"
                     /locus_tag="ACGE4T_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8386468.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16969..18195)
                     /locus_tag="ACGE4T_001179"
     CDS             complement(16969..18195)
                     /locus_tag="ACGE4T_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8386469.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTSDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(18267..18569)
                     /locus_tag="ACGE4T_001180"
     CDS             complement(18267..18569)
                     /locus_tag="ACGE4T_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386470.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
BASE COUNT         6057 a         3413 c         3948 g         5309 t
ORIGIN      
        1 tttaaaaaaa tgctattgac agtaagcgtt ttccataata tgatcaaatc gttgggaacg
       61 ctcccaaaaa agaaagggga atccgatgta tacaaaactt ttacaagcga tttatcgcga
      121 ctcagatttg tctgagaaaa ccgcctctat gattgaacaa aaagtcagtg tttatcagca
      181 gaagaaaggc caggcaggca aactgcattg ggacgaagaa gatattttct tgattactta
      241 tggggatcaa ttttttgaag agcagcaacc aactttgcgt acatttaaga aattttatca
      301 aaagtatttg gcagatacat ttagagttgt acatttcttg ccatttttcc catatacatc
      361 tgatgatggt ttctccgttg taaattatca ggcaatcaac gaccgtttgg gcacatggga
      421 agatatagaa gaaatgagaa gcgaaacacg gttaatgttc gactttgtct gcaatcatat
      481 gtctgccgaa agtacttggt ttcaggaata tttaaacttg aatgatacgt acaaagattt
      541 ttttattgat ttgccactgg atacagatac aagcagcgtg actagaccaa ggaccagccc
      601 gttacttaca acgttcattg acaaaaacgg gaaagaaaaa cacgtatgga cgacttttag
      661 cgaagatcaa gtagacttga attttaaaaa tccagaagtt ttattgaaaa tgatcgatat
      721 tcttttgttt tacttagaaa aaggcgcttc atttattcgc ttggacgctg tgggattttt
      781 atggaaagaa ctaggaactt cttgtataca cctgccagaa acgcataaaa tcatccaatt
      841 attccgtgca atcgctgatg aagcagctcc aggaacggta ttgatcactg agacaaatgt
      901 gccacacaaa gataatatca gttattttgg gaatggcgag aatgaagcac agatggtcta
      961 tcagttttcg ctaccacctt tgacccttaa gcgattcgtt cgggtaatac aaataagttg
     1021 aaagaatggt taaaaaccat cgatttcgca tcgaaaaaaa caactttttt taatttttta
     1081 gcctcccatg acggtatcgg attgaatccg gtacgaggta ttctgccgga aaaaacgatc
     1141 cttgaattag tggaacagct gaagaaagag ggtgcccgag taaactacaa gaaaaaccca
     1201 gatggaacga ccagtccata tgagataaac gctacgtatc tagatgcgtt aagttctcca
     1261 acagatacga cggaatggag aagcaaacgc tttttagctg ctcattcatt tttggtaacg
     1321 ttaccaggcg tgccggcaat ttatattcaa agtgtgctgg gaagtcgaaa tgattacaaa
     1381 ggcgtagaaa aaacaggaga gaatcgaaaa atcaatcggc aaaaattttt gttagcagat
     1441 attgagaaag agttgcagac agatccatta agaacagcag tatatggtga attgacccag
     1501 atgctatcta ttcgaaaaaa agaaaaagcc ttccatccga atagtttgat ttacttactg
     1561 gactcagcag attcttttat cagttttatt cgcgaagcga aggaagagaa agtcttagtt
     1621 atccataatt tttccaatca atcagcaggt tatcttttac caaaaggaga ttacctagac
     1681 ctgttttctg gacaaattat ccattgcagt caagaacgaa agttgatatt ccagccgtat
     1741 gaatatatgt ggctgaaaat aataaaataa gaggtggaaa aaaatgaaaa aaattattgt
     1801 tactacctgt ctgctagcta ctggatttct tcttactgct tgtggtgaaa gtcaaagctc
     1861 taacgaatca caacagacaa cgattcaatt catgcactct tctgtcgaac aggaacgatt
     1921 aagtgtgatc aatgaactgg ttgctgattt tgaaaaagaa aatccagaca tcaaaatcaa
     1981 gcaagtgccg gtagaagaat cagcgttcaa tacaaaaatc gttacgttag ctcgttccgg
     2041 taaattacca gaagtaatgg aagtcagtca ggattttgct aaagtgatgg ataaagatga
     2101 actgattgat cagacggcag tccaaaacgt tatcgaggaa gctggagaag ataattatta
     2161 tgacggagcc aaaaaccttg ttcgttcaga agatggaaaa agctatattg ctgcaccaat
     2221 tagcggctgg gttcaaggta tttggtatga caaagaaaaa ttatcagaag ctggtttttc
     2281 agaaccagaa aattgggatg atattttaaa aattgctcaa catttcacag ataaagagaa
     2341 taaacaatat ggaattgcta tgcctactgc tgaaggaaca atgagtgagc aagcgttctc
     2401 tcaatttgct ttgtcaaatg gagcaaatgt tttggatgat gaaggcgagg taacgatcga
     2461 tacggaaaag atgagagaag cattgtcgtt ttatcaaaac ttatcccaat acacgatgcc
     2521 aggctctaat gatgtgacag aaatcaaaga tgctttcatg aatggaacag caccaatggc
     2581 aatctattca acttacattt tgccttctgt ttatgaagcg gggaactcag aaaatatcgg
     2641 ttttgctatc ccaacacaaa aagatcaagc tgtctatggc acagtatctg gattgacgat
     2701 ttcttctggt ctagacgaga aacaaaaaga ggctgctgaa gcattcacag ctttcttatc
     2761 tcaaccgaaa aacatggaaa agtgggtctt gatgtctcct ggtggtgctc aaccagtgaa
     2821 taaacaagta gtcgaactgg atggttacaa agaaaatgaa gtcatcaagt cttttggcga
     2881 gctgccatct gagattgcta gtgcttttga tgaagtacaa gtctttggac tagtcgggga
     2941 aaagaatttc acgaaaatgg gagatatcac aagctcaggt gtgatcggaa aagcagtgaa
     3001 ccaagtaact gtcggaaacg aagatgtcga tcaagcttta gctgacgcac aaaaaaatac
     3061 gaaataacaa gaagttctgc caatggtgtt cgaaaaaagt agatagctat tggcagatta
     3121 tcagggggag gtttcaatga atcgtccaag gaaacattct gacttaaaat tagccatggg
     3181 acttttagca ccaagcatgc ttttattagc tgttttagtg ctgattccaa tgctttccaa
     3241 tattcagatc agttttttcg atatgccgct gaatcctaac tcagacgccg tttttatcgg
     3301 attaagcaat tatcttgaaa ttttaagtga tagtgaattt tatcgttctt taggtttaac
     3361 agttgcttac acattcttag ttgtagcagg cagtacagct ttaggattag ccgtagctat
     3421 tttgcttaac cgcaagttca gatttaggaa aacggtgcgt tcactgattt tattaagtta
     3481 tgttacgcca tctatttctt tgatttttgc ttggaaatat atgttcaata atagttatgg
     3541 aatcgtcaat gatctgttag tagataggtt gcagcttttc aacgaagcac cgctgtggtt
     3601 cgaccagcca gttgctagct tctttatggt cgttttgttt gctatctgga gatactatcc
     3661 gtatgcgttt atttcatttt tggctatttt gcaaacagtc gacaaaacgc tttatgaagc
     3721 agctgaaatg gacggtgcaa atgcttggca gaagttcaag atcgtgacac tgccggcaat
     3781 caaaccagta ctagcaactg tgattgtttt acgatctatc tggatgttct atatgtttac
     3841 agatgtctat ctactgacga ataaagtcaa tatcttaggc gtttatttgt atcagacagc
     3901 ctttgcgttc aatgatcttg gaaaagcagc agctatatcg attctattgt tcatcattat
     3961 tgcagcggtc gtcttgctct cacgaaagaa ggtggacttg aaatgaaaaa taaaaagagc
     4021 aggaaaatct tattttacgc gacgattttg ttgctattag ctgtcaattt gtttccattt
     4081 ttgatcatgc tgatgacatc attcaaggat tcacaagaag caattgcaac gacacaaact
     4141 atttttccga aagaatggac ttttgaacat tatcaggata ttttcaatgc tgagattttt
     4201 ccattcatca tgtatttcaa aaatagttta gtgatctcct tgatagcctc cgtaactgca
     4261 gtagtgattg ggatcagcgg ggcatacgct ttgtcaaaac tacgcttcaa agctcgctcg
     4321 aaaatcaatg ctagctttta caccgtttat atgttttccg gtattttgat cgttgttcca
     4381 ttatttaaaa tcatttccgg tctaggtttg tatgatacca atacagcact gattatcacg
     4441 atggtcgttc aaacacttcc tactgcgata ttcatgctaa aaagttattt tgacacgatt
     4501 ccgactgata ttgaagaagc agcaatgatc gatggtctta gccggatcca gatcattatt
     4561 cggatcatta taccattggc tatttctgga atcatttctg tcttcgttta ttgctttatg
     4621 gtttcgtgga atgattattt atttgcttcg attttcctgt cgacctccga aaaatttact
     4681 ttaccaatcg gtttgaacac attattcagt acaccagact acatttgggg acgaatgatg
     4741 gccgcttcat tggtgaccgc tttaccagtc atcattatgt atgcgttatc tgaacgattt
     4801 attaaaggtg atttagtcga aggcggcgta aaaggataaa aagaaaagaa agaggaaaga
     4861 aaagatgaaa aaattagttg cattagaacc aagaatagcc ggattcaaag attacgaaga
     4921 ccgtgagatc gaaaagaacg aagtacgtat ccgagtaatt ttcgctactc caaaaccagg
     4981 aacagaaatt gtcgacttta gaggattaag tccatttatc gatgaagaat tttcagaaga
     5041 atggcgccta ttcttgccaa gagaagaagg aagccaacgc ggtgtggtat ttggcgaatt
     5101 tcaattagga aatatgattg tcggtacgat cgaagaaacg ggcagtttgg tgacagagta
     5161 tcaaaaagga gagttagtct gcacttatgg acccattcgt gaaacgctga ttacaaacgg
     5221 tgttgataat tataaactac gtaaacttga tcggttggat caatggaaaa atgcagtatg
     5281 ttacgatcct gctcaattcg ctttaggtgg tatccgggaa gcacatgttc gagcaggtga
     5341 tgctgtagca atcttcggtt taggcgctat cgggcaaatt gcgattcaat tagctaaaaa
     5401 agcaggtgct actaccatcg ttgggatcga tcccatagcg cagcgtagag aattagcaga
     5461 aaaacacggt gccgattaca cgctagatcc tatgaaagca gatgtcggtt ttcgtctaaa
     5521 ggaattgaca gataaacgtg gagtagattc agttattgaa acgagtggca gtgctgccgc
     5581 attacaagct tctttaatag ggatcggcta tggcgggatc atttcttatg ttgcctttgc
     5641 taaacctttt ccagctgggt ttaatcttgg tcgcgaagca catttcaata atgcaaaaat
     5701 cgtctttgct cgagcatcta gtgaaccaaa cccagattac ccaagatgga accgtaaacg
     5761 aatcgaggac acatgttggg aattattgat gaacggttac ttggattgtg aagaaatcat
     5821 tgatccagtt gtgccatttg cagaaagcgc agaagcttac atgcgttatg tagaccagca
     5881 cccagaagaa agtatcaaaa tgggcgttca agtccgagga ggagaacaat gaaattcggt
     5941 acgcaagatc aagcattttt cccaagagac attttagaaa aatttagatt tatcaaagac
     6001 tgtggatttg aagaatacga aatcgacggt aagttgttaa tagaaaatat cgacacagtc
     6061 aaacaggcaa tcaaagaaac agggatccca gtagtgacag cttgtaacgg ctacgatggg
     6121 tggatcggtg actttatcga agaacggcgt cttaatggtg tacgtcaaat tgctgagatt
     6181 ttgcgtgcac taagcgaggt tggaggcaaa ggaatcgtta tcccagcagc ttggggaatg
     6241 ttcacttatc gattaccgcc gatggtttca ccccgaagca aagaaggaga ctataaagca
     6301 gtcagcgaat cacttcgtta tttagataaa gtagccgaag aaacaggtac ggttatttat
     6361 ttggagcctt tgaaccgata ccaagaccat atgatcaatt tgttagcgga tgcgcgtcat
     6421 tacattgact ctaatcaatt gaaacatgtg cagatcatcg ctgattttta tcatatgaat
     6481 attgaagaag atgatatttc aaaagcattg actacgcaaa aagagtatgt gggtcatatc
     6541 catttagcag ataatcatcg ctaccaacca ggatctggcg caattgattt tactcgccat
     6601 tttcaaacgc tacttcaaaa tgagtatgaa ggttatatgg tatatgaatg tcgtttgcga
     6661 ggagaaaatg aagtgaaaat ttatcaggaa tcattgcaat acttgaaaaa ctgtctggaa
     6721 acagcgcgtc atttaaatga tttagagaaa gaatagagaa agaataacaa taagtaaata
     6781 agcaaaggag ggagaaataa atgaagaaag tagcaatcat aggcgctgga caagtagcag
     6841 aaaaagtaca tgctgcttat tatcaaaaaa gatcagatgt cttagaattg gccgctgtgg
     6901 ttgatccagt cagccagcga gcagaagact tttgccgacg tacaggattt tctcatgcgt
     6961 atacatcagt tacacaaatg ctgaatgaga tttctcccga tatcgtgagt gtctgtacac
     7021 ccaatcgctt tcacttcgaa catgtattgg ctgccctcga agctaatgct tcagtgatgt
     7081 gcgagaaacc accagctatg acagctgatc aagcaagaaa gatggcagag acagccgata
     7141 aaaacgggca gattttggct tatggattcc agcatcgttt ttctgacgaa gcaacaatga
     7201 tcaagaatca tcttgatgtt ctcggggata tttattacgt agaagcgaat gcgcttcgtc
     7261 ggtctggtgt tccaggctgg ggaacgttta tcgacaaaga acttcaagga ggcgggcctt
     7321 tgatcgatta tggcattcac atgcttgata cagcgatgta tattactgga tttcctaaag
     7381 taaaaagcgt tcgggcgtac agcttccaaa aaattggtac gaaaaaaaat tcagggaaat
     7441 tcgggaaatg ggatccaagc aaattcacag tagaagatgc aatttttggc acactggaat
     7501 tagaaaacgg caagatcatc caactgaata cttcgtttgc tttgaatatc agagaagaca
     7561 aactgaatgt ggctttttgt ggcgatcagg caggtgctaa tctgtatcca ggcgaactat
     7621 tcgaagatgt agatggaaag ctggtttcct tacaaaagac cacgccagtt aatgacccac
     7681 atgaactaag tatggctcat tttgtgaatc gggcgctcgg tgaccagact cagatgattg
     7741 cagatggctg gcaaggatat gtcatccaga atctggtgga aaatttatat cgttcggcag
     7801 aaacaggaga agttattacc ttatgaaagt agaagaaaaa cagtttgatt tagagtatat
     7861 caataagtat gcgagtcaga tgaccattgg aaatggattt tttggtatca gaggaagcca
     7921 ggaggaagac tatcggaatc aagtaagggg aatgtttgca gctggtgtat ataaccgacc
     7981 tttaggagca gaatcagccg agttagtgaa tctcccagat gtcatcagat gggaaataaa
     8041 aatcgacggt gaacattttt ctcttgagac gatgaaagtt cttgcatacg agcgggcact
     8101 agatttagaa atgggagagc ttaaacgcta tgcgctcatt gaaactgctc aaggaaaaaa
     8161 agtggaagtc attacttgtc gtgttgctga tcaaaataat ctccatcagg cagccctgaa
     8221 agtggtcatc aagccgttga ccgaagactg ccaagtagat gtgaaaacgg gtatcgatgg
     8281 acagcaaacc aattttggta cacagcagtt ggttgaaaaa gaactgcgag tatttggtga
     8341 cgatttgatc agtgcatcct accagacaac agaaagtaaa attgatattg ggattgcagc
     8401 taaattcaat aaagcaggtg tattccaagc aaaaaatcgc cggatcaccg cttccttttc
     8461 agaaaagatc agaaaaaata cgtcctttac gctggagaag atcatcgtgc tctctacctc
     8521 tcttcagcaa acagacccgt ttaagaaaag tattgcccaa gcccagaaaa gcgttagtta
     8581 tacagaaatt ttagagcagt cgatgtctta ttggacagat ttttggcatc agcatcggat
     8641 aacgatccaa ggcgattctt ctttcgatca gttagcgatg gacttcgctg cttaccattt
     8701 acagattatg actccccgca atgatccaag tgttagtgta ggggcgaagg gactaactgg
     8761 cgaaggttat aaagggcatg ttttctggga tacggaaatt tttatcctgc ctttcttttt
     8821 atatcagttt cctgaagtag caaagaattt gctgctgtac cgttatgatc gattagaagg
     8881 cgcacggcaa aaagcaagag attatcacta tcaaggtgca cttttcccat gggaaagcgc
     8941 acacactggt caggaagaga cacccaaatt tgctgcattg aatatcaaaa ccggaacaag
     9001 acaagaagtt gcttcagctg ccgcagaaca tcatatcagt gcagaccttg cttatgccgt
     9061 tcaagagtat gtccaagcaa cagaggatca atcgttttta gaaacaaagg gaaaaacgct
     9121 cattcatgaa acagcagctt tttggctgag cagagctgta aaagtcgatg atcatttaga
     9181 gatacacaga gtcattggtc cagatgaata cacagaatat atcgacaata atgcatatac
     9241 gaactatctg gcgcattata atgtggcctt agctgttaaa tatcaagctg gcgatccgct
     9301 ttttttgaaa gaatgtcaaa attttttaga taagttatat ttgccagtgc ctaacaaaga
     9361 acagctgatt ccgcaagatg acacgtatct cagtaagcca gttattgatt tgaccactta
     9421 caaagaacgt cctggcagcc agtctatttt acttgattat gcccgtagcg aagtaaatga
     9481 actccaagtc agcaagcaag cagatcttgt catgctgtta tacttattac ctgagtattt
     9541 ttctcaacca atcgtttcta agaatttgca ttactatgaa aacaaaacaa tccacgactc
     9601 atcgttgagt aaagcgattc acgccataga ggcttgccgt atccaagaaa tggaatgggc
     9661 gtatcattta ttccaagaag cgtgcctgat tgatttagga ccagatccgc attcatcaga
     9721 tgacggaatt catgctgctt cattaggagc actatggcta gcagttatat ttggtttttc
     9781 aggtattaca gtaacgaatg gaactctttc tatttctcca agacttcctt cagcatggga
     9841 aaaaatttct tttcctttta tttggaaaaa tcacaggttg ctatttgttt tgacaaaaaa
     9901 acaaatagta ataaaaaaag agacgaaaga aacattacct atttttgtca atggagacca
     9961 ataccagctg gaaaaagaac tgatcttgga acggagggga aaataatgcg tattctgatt
    10021 gcgccagatt ctttcaaaga aagtctgaca gccttagagg cagcacaagc cattcaatca
    10081 ggttttcaag aagtttttgt tcaggctgaa tatgatctgc tacctatagg cgatggagga
    10141 gaaggaacgg tttcatcatt agcagacagt ttaaggttga agcggcaaac agcagtcgtt
    10201 actaatgcat ttggcaaaaa caaagaaatc cagtacgctt cagacggtca aactgcgatt
    10261 tttgaaatgg cagaaatcgt cggattgaaa gatgtcccac taaaccaaca acagccactt
    10321 aggttatcca cgcgaggagt aggagaactg atacgacaat tgttgcaaca aggtgttcga
    10381 cacatcatca tcggcgttgg cggaagtagt accaacgatg gcggcatcgg catggcagaa
    10441 ggtctaggtt atgcttttta cgatcaagat aatcaaaaag tagaagcact gggagaaaat
    10501 ctaggtaaaa ttgctacagt tgataaaacg aacgtgccta ttgagctgat taaagccaaa
    10561 atcactgtca ttactgatgt tactaatctt ttatgcggaa aagaaggcgc ttcttttgtt
    10621 ttcggaccac aaaaaggctt gaaagaaaat gaattagcag tggctgacca agccatgcat
    10681 gatttttata tgagatttgc ccctcaagta atcgaggcat cgggcagtag tgctggtggt
    10741 ggaatgggtg caggactgtt agcatttgct tcagggaaaa tagtctctgg catcgactac
    10801 gtattggatt tactggagtt cgatacgggt acagcaagca gacctcgtaa tcgtaggcga
    10861 aggacgtcta gatggacaaa gtctaaaagg aaaagcgcca gttgggattg ctagaagagt
    10921 accagagggc gttcctgtga tagctatttg cggaagtgtt ggtaaaggaa gcgaaaaagc
    10981 agcagatgtt ggcatttcgg ccgtttttcc gatccttcct gcagctgtgc ctttacaaga
    11041 agctttacag caagccactg ataatcttca tcgcacagct aaaaacatcg catctttact
    11101 tcaagtcgtt cattttcaag gaggtaatcg atgaaaatca aagcagcact attcgattta
    11161 gatggcgttc ttgtagacac agctcgttat cattacgaag cgtggctggt tttagctaac
    11221 cagctgtcca ttccattcac tgagaaagaa aacgagcaac tgaaaggaat atccagaacc
    11281 gagtcactag agcgattgct gtcatttgga aaaatggaac agaaattctc agaaaaagag
    11341 aaatcagcct ttgctgagca aaaaaacaat ctatatcttc aagcgattca aaaaatggat
    11401 gaaacatccg ttttgcctgg tgcaatagct gttcttgagt acttgaaaaa aacaaacatc
    11461 aagatcggat tgggatcagc aagcaaaaat gcacgcctca ttttagaaaa aacgaatttg
    11521 acaagttatt ttgatgtcct tattgacggc acgcaagtat caaaagcaaa acctgatcca
    11581 gaagtttttc tcaaaggagc acagcaattg aatgtaccgc caaatgcatg cttggtcatt
    11641 gaagattcag aagctggttg tcaagcagct ttggctggta acatgcatgt attgggaata
    11701 ggtgaaaaca taaatcttcc ttctgctgaa tatgtgattc ctgatttaac cgtttttgat
    11761 caagtacgtt cgttttggca tttatcagaa gccgtatcac gtatataata gtttccgttt
    11821 tttctgtcac aagcaaaacg ttatcctcct tttggaaatc aaggtatgat gagaaaaaag
    11881 ggggatttta tatgacaaaa aaagcagttt tcgcaggcgg atgcttttgg tgtatggtca
    11941 aaccatttga agagcagcca gggatcattt ctgtgacatc cggttatact ggcgggcatg
    12001 taccaaatcc aacttatgaa caagtaacaa caggcacaac agggcataca gaagcagtag
    12061 agatcgaata tgatccagaa gttatttctt atgatcagtt ggtggaaatc tattggcagc
    12121 aaaccgatcc gactgacgca ttaggacagt tcgctgatcg cggtgattct tatcgtccag
    12181 tgatctacta taatgatgaa gaagaacgaa atattgcaga gcgttcaaaa caagcattgc
    12241 aagacagcgg gcgattcgat aagccaatcg tgacaaaaat cgaaccaaga agcgaatttt
    12301 atcctgcaga agattaccat caggattatt acaagaaaaa caagctccat tacaatcttt
    12361 atcgagaagg ttctggacgt gcaggtttcc ttcgtcgaca ttggggcgtc aagaaataag
    12421 aagccgaaaa agctacttat ggaacaccgt atatcagagg ttgtaaaacc agcattttga
    12481 gctaatactg agggtcagct gattgaacac catttagtgg agaggttgtg aacctgtcgc
    12541 ctagctccaa gtcacaagga tttggtaaga gaacactata tatcagaggt tgtgacaagt
    12601 cgtttgtcac aacctttttt caatacctaa gatgaaggac aatcagcaac gcactgctca
    12661 aaaaaggaat gaaaggcagc ttttccaact tttttttatg acagataaag taaacagaaa
    12721 aaacggatat tccaaacaga ctagcaagaa acaagatctg aagcaactct tctagagaca
    12781 agccaccact ccaagaaagc atcaacagag tatcgccagc tccgagatag gattgaccat
    12841 agcgaaggca cacaccgcct acgataaacg ttaacagcac actttgccat tggaagacgc
    12901 cggcctgcaa ccaaaaaatc cataaaattg cccaagaaca gaatagcgtg tgagaatgga
    12961 ccatgaagta aaataaatcc atcaacgaaa atagcaaagc cgaaagcatc catatgaacg
    13021 tcgaaaaatt tcttgcggaa gaaaaagtaa agaaccaggc aaaaagacca ccacataagg
    13081 cttctgccaa caaatagtag aagggcaacc gacacctgca atagcggcag cgaaatcgtt
    13141 ggacgatgat cgagagaatc ggaatcaatt cataccaaga aagcgaacga cagcatttga
    13201 cacaatgaga tctaggataa ataatagagt gacctaacgg aatccgctgg gcggttaaac
    13261 ataaaaatga tccgatacag caaccaatga taaaataaag taacacgcca tctccccctt
    13321 ttcaaaacca agatacgcaa gcttaaacta aatttttgta catacataaa atttgaggtg
    13381 aaaaaatgaa cgtttcaatc ggattagata tcggaaccac acaaaccaaa gcagttgctt
    13441 tcacagatga agcccaagaa gcagcatccg cttattttcg ctaccctttg atccaagaaa
    13501 cacaagggat ggcagaacaa gatcaggaat tgatctttca aggtgtctgc accgttataa
    13561 aagaagtaat taaacagttg ccttcagaca caatcattca atttgtctcc ttctcctctg
    13621 cgatgcatgg cctgatattg ttggatgaag agaaaacacc gttatctcga atgatcactt
    13681 gggcagataa ccgagcagcg gacgaagcag aaaaactgaa atataccgtg cttggcaaag
    13741 aattttatcg gcagactggg acgccgattc atccaatgac accattgaac aaaatcaagt
    13801 ggctgcaaaa cagccaacct gaattattca aaaaaactgc ttattttgtc ggtatcaaag
    13861 actatatctt ttatagattg tttggcgaac tgatcagcga ctacggaaca gcttcaggaa
    13921 ctggatattt tgatatccat gaattccaat gggcagaaga agtgcttgac tacttgaaca
    13981 ttcgaataga gcaattgccg caagtctttc cttccaccta tcaagtaaca ggtctaccat
    14041 caaaaacagc agcagaacta ggtttgccaa atgatatatc atttgttttg ggaggggcag
    14101 acgggccgct ttctaatcta gggttaggtg cttttggtga tacgatcgcc gctttgaccg
    14161 taggaacgag cggagcactt cgttttatta ctactcagcc tcagttccat ccagaaatgg
    14221 agacgttttg ttatgttctg gatcagaaac attgggtagt gggaggagcg actagcaatg
    14281 gtgctggcat ctttgattgg gcagggcata catttttaca ggaagtcgtt caatctgcta
    14341 aagaaaaagg agaaaatcca tatgatgcac tgctttcggc tatcgctttt gtgccagcgg
    14401 gtgccaatgg tctgattttt catccctatt tacttggtga gcgagcaccg ctttgggacg
    14461 cagatgcgtt tggcagtttt atcggtttac agcggcaaca tacagaaaaa gaaatgatga
    14521 aggcgattct agaagggatc tgtctcaacc tttatcggat tttaaaagga gtttgccagg
    14581 aaaatcagct cacagagatc cgtgctacag gcgggtttgc tcaatctccg atttttcgtc
    14641 agatgatggc tgatgtactg ggttacccgc ttgttttccc tactgtcaca gaagcatccg
    14701 cattaggagc tgtgatttta ggctggagaa gcatcggaaa gatccaaagt atcagtgaag
    14761 cacaggaatt gatagaattg aaagagcaag ttggagtaca agaaactgat cataaaaaat
    14821 atcagaaaat ctatccatta tttgtttcga cacaaaagca tttagcacag acttatcagc
    14881 aatttgctca actgcgagaa gaattagctg attctgaaaa atgattggct ttacggataa
    14941 aaaagcgaac tgaaaatcgt tctcaggtaa atgattttgt tgtacttttc attgaaagtc
    15001 tactattatt ttaagtgtaa gaaaatgtta ggaggcaaga aagatgaaat tccaacaaac
    15061 caaagaagta ttaaatcaat tagttgcaga tctaagccaa atgtctgttg ttgttcacca
    15121 aacacattgg tacatgagag gaccaggttt ccttacattg cacccaatga tggatgagtt
    15181 catggatgat ttgaacgctc agttagatga agtatccgaa cgcttgatca cattagacgg
    15241 ttcaccttat tctacattgc gtgaatttgc agataacaca aaaatccctg atgaaattgg
    15301 ccgctgggat cgtactattc cagaacgttt ggaaacactt gttgcaggct atcgttatct
    15361 agcagatctt taccaaaaag gaatcgaagt ttctggagaa gaaggcgacg atccaacaca
    15421 agatatcttt atcgaattca aaacagccac agaaaaacgt atctggatga tccaagctca
    15481 tttgggcaaa gcgccagaga tcgacgcgta aaaactgttg ttgaacttga ccgaaaaaga
    15541 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacgtcaaa atagttcctc
    15601 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttaccaag
    15661 aggttgtaag cctgccgttc agctccgagt cacaagaaac aattttaaaa aacagcttct
    15721 tatgttttat ggatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttattc
    15781 aaaataaggg gttgtgacaa gacttttgtc acagcctctt tttgtttttc attttgacag
    15841 gaaacgaccc ttttatataa aagaaagaaa agaagtaaag tttcatataa atttttcaat
    15901 caagcagaaa aacgttgaca ttgactagat tagcgggtaa tatgttaaat ggtattgttt
    15961 gccccacaaa gagccccgga aactcgaagt gtatgtcaaa catcagaact taaattggag
    16021 gaaaataacg tgcgtacaac atatatggcc aaaccaggcg aagtagaacg taaatggtat
    16081 gtagtagacg caactgatgt tccattggga cgtctttcaa cagttgttgc atctgtacta
    16141 cgtggtaaaa ataaaccaac tttcacacct catttggata ctggcgattt cgtcatcgtc
    16201 atcaatgcag acaaagtgaa attaacaggt aaaaaagcga ctgacaaaat ttattaccgt
    16261 cacagcaact acccaggagg attgaaatca atcactgctg gcgaattgcg tgctaaaaat
    16321 tctcgtcgtt tgatcgaaac ttctgtaaaa ggtatgcttc ctaaaaacac tttaggccgc
    16381 aaacaattta caaaattgaa cgtatacggt ggagcagaac atccacatgc agcacaacaa
    16441 ccagaagttt tagacatcac aaacttaatc taataggagg gaatttcatt ggcacaagtt
    16501 caatatatcg gcacaggccg tcgtaaaaac gcagttgctc gcgtacgtct agtaccagga
    16561 actggcaaaa ttactgtaaa taaaaaagat gttgaagaat acattccaca tgctgacttg
    16621 cgtgaagtaa tcaatcaacc tttcgctgtt actgaaacaa aaggcgcata tgacgtattt
    16681 gttaacgtaa acggcggagg atacgctgga caatcaggag ctatccgtca tggtatcgct
    16741 cgtgcattgc tagaagttga tcctgacttc cgtttagctc ttaaacgtgc tggcttactt
    16801 actcgtgacg cacgtatggt cgaacgtaaa aaaccaggtc ttaagaaagc acgtaaagct
    16861 tcacaattct caaaacgtta atatccttta tatatcaacg tttctggcac ttttcaatta
    16921 tttggaaagt gcttcttttg tttttaagaa cacatttaag aacacatatc aatcacactt
    16981 attcaatcct ctctgaaaag cttcaaaaac agccttatca actatatctg gtgtgttcac
    17041 atatatcttt gttgtagaaa tatctgagtg tgttaaagca tttgaaattt gattcatatc
    17101 tgctccacct tgtctagcta tagttgcata agtatgacgg agtttgtgtg gagataaatg
    17161 aactaagtca ggatgccttc ttttaattga attgattcga tagtttaggt aatcggcatg
    17221 aacaggacaa tttacttctc cagatggttt agtataagta aagagatact gatctgacgt
    17281 ttgcttaatt tttaatttca acaattgatc agcttgtacc gatttccatt cacttagcaa
    17341 tgtcattaca acttctggta cttgaatgac ggtatctttt cgaccttttg taaacttttt
    17401 tctttgatat ttatctaaag tatgtttaag acggaccgtt tgtttttcaa aatcaatata
    17461 tttccattgc aaagcgtaag tctcactttt tctatcacca agatacaggg taatcataaa
    17521 aagtgtgtaa tcatgaaaag taagtaaatg attattaaaa tcatcattta ctgcttctat
    17581 ccaattagta agttcttttg atgatagtgc ttgcttacct tcttgtattc ttttttcttc
    17641 taaagcattt tttttaggtg cagtgatact ttgaataatt ttagtagttc gattaaattc
    17701 gatataattt agtatttctg caatatcgaa catggaacga acatatcctt ttatgatttt
    17761 tatatttgca tattctttcg attttttggt taattcatct gatattatct cagtattgat
    17821 atttagataa ttcattgcgt attcaccaaa cataggtaaa atatgcaacc gaaatatatc
    17881 ttttgtatta ctaatggtta tatcactagg aggcctgttt gatctaattg tttgacctaa
    17941 ttcatatcta ggaagccatt tagattcgta aaatttttta aacgttattt ttccttttaa
    18001 ttctaatgag tttgcattct cctctctcaa aactttttct attttcttct tcatatcatt
    18061 ttcggcttta atcgccaatt gcctatttgg aaatgttttt tgatactttt ctgatgcaat
    18121 tcctgttatt tctttaaacc tttttggata tctcagatta gttttatatt tcccatttga
    18181 aagctttgta ggcaaaatcc tcactcctta aaaaaagtaa ggcagtatat ttcactaatt
    18241 ttagtatatg cggcgagaag aaaatttcaa gttattttaa atgtattcat aaagtcttta
    18301 atatcttcta cgtcaaagcg aactacacca ccaactctta ttactgggaa attatatata
    18361 cgaatccact tatctagagt attattatcg attcctagaa atgcacaggt ttgtttttta
    18421 ttcaggtaga ctctggattc tataaaatca tctcctaaaa tagtagttat gaaatttttg
    18481 aattgctttg ggtaagcaca taggaatttc acctctcccc agtttacggc gagccttacg
    18541 gaagtatcat tatttgctgc tattgtcatg gcattttgtt ttcccagaac aaaaagaaat
    18601 ctctaagtat ttgtcgctaa tcaggtcatg gcgtacttga gtttctagct cgaatagcag
    18661 ggatcgttat tcaaaaattg tatttaattg tcaatgatca aaaatagttt ttttcctatt
    18721 cactatt
//
LOCUS       DAXWLG010000043        18432 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXWLG010000043 DAXWLG010000000
VERSION     DAXWLG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.043"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(343..798)
                     /gene="tnpA"
                     /locus_tag="ACGE4T_001181"
     CDS             complement(343..798)
                     /gene="tnpA"
                     /locus_tag="ACGE4T_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFF8386471.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1041..2467
                     /locus_tag="ACGE4T_001182"
                     /pseudo
     CDS             1041..2467
                     /locus_tag="ACGE4T_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2656..3324
                     /locus_tag="ACGE4T_001183"
     CDS             2656..3324
                     /locus_tag="ACGE4T_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFF8386472.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(3524..3910)
                     /locus_tag="ACGE4T_001184"
     CDS             complement(3524..3910)
                     /locus_tag="ACGE4T_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386473.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(3882..5264)
                     /locus_tag="ACGE4T_001185"
     CDS             complement(3882..5264)
                     /locus_tag="ACGE4T_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8386474.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(5420..6607)
                     /locus_tag="ACGE4T_001186"
     CDS             complement(5420..6607)
                     /locus_tag="ACGE4T_001186"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFF8386475.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(6614..7645)
                     /locus_tag="ACGE4T_001187"
     CDS             complement(6614..7645)
                     /locus_tag="ACGE4T_001187"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8386476.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(7661..9115)
                     /locus_tag="ACGE4T_001188"
     CDS             complement(7661..9115)
                     /locus_tag="ACGE4T_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFF8386477.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            9290..10195
                     /locus_tag="ACGE4T_001189"
     CDS             9290..10195
                     /locus_tag="ACGE4T_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFF8386478.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            10286..10963
                     /locus_tag="ACGE4T_001190"
     CDS             10286..10963
                     /locus_tag="ACGE4T_001190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8386479.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            10960..11130
                     /locus_tag="ACGE4T_001191"
     CDS             10960..11130
                     /locus_tag="ACGE4T_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386480.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            11244..12140
                     /locus_tag="ACGE4T_001192"
     CDS             11244..12140
                     /locus_tag="ACGE4T_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8386481.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(12195..13565)
                     /locus_tag="ACGE4T_001193"
     CDS             complement(12195..13565)
                     /locus_tag="ACGE4T_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8386482.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            13803..14549
                     /locus_tag="ACGE4T_001194"
     CDS             13803..14549
                     /locus_tag="ACGE4T_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8386483.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            14546..15385
                     /locus_tag="ACGE4T_001195"
     CDS             14546..15385
                     /locus_tag="ACGE4T_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8386484.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            15386..16336
                     /locus_tag="ACGE4T_001196"
     CDS             15386..16336
                     /locus_tag="ACGE4T_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8386485.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            16477..17229
                     /locus_tag="ACGE4T_001197"
     CDS             16477..17229
                     /locus_tag="ACGE4T_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386486.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            17248..18399
                     /gene="queG"
                     /locus_tag="ACGE4T_001198"
     CDS             17248..18399
                     /gene="queG"
                     /locus_tag="ACGE4T_001198"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFF8386487.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5982 a         3462 c         3679 g         5309 t
ORIGIN      
        1 atcaatactt acgtaactca aaaatacttc caaaggtgtt cgataatcca gtgattttct
       61 aggaagatta tttctgcgcc agcattttct aatgtttgaa tttgtgtttt gtaatcttgg
      121 ttgcttgtgc taattcttga atagccgtaa attgccaaaa tttcatcacc ctttctggac
      181 ataagtttct gacaccagtt aaaatcaagt ctatcagtaa cccatacacc cgtgaggacg
      241 ggtgtatttt taatccccct agaaggggtc gacactcgct gccccttaaa aggggatttt
      301 taaaagaccg ccaactgctc atcttcactc acttacttct ctttattttt taaatggttc
      361 tacgtactct cttttactta taatatatcg aatgcgatca tcagcttctt gttcacggat
      421 gtatttttca tttgtctttt gattcaatcc gactgtactc acatagtatc tcttcgacca
      481 aaaggatttg tttccgtatt tatatttgag atttgcgtgc tgctcgtgga ttagtaccgc
      541 actacgacct ttcagatacc ccataaaact ggatatacat atttttggtg gtactcgaac
      601 aagcatgtga atatgatcta gcattgcatg tgcttctatg atttccacat ctttcatttc
      661 gcacaacttc ctaagtattt ttccaagatc ttttcgtaat tgcccataga ttacttttcg
      721 ttgatatttt ggtgtaaaaa taatatgata cttacaattc caacgagtat atgctaaact
      781 tttatcgtca tttgacatga aaaaactcct ttcgatctga tgagcggttg gtagccactc
      841 actagtatat cgaaaggagt ttttatcgct aaagatactt cttccacgcg tagaaacgcg
      901 cggttttctt gtttcgcacg ctttacgcac aaaactgact gaagtcatta agaaaataaa
      961 agggtctaaa acttttaagt tttgtaccga cccccaaaag ttagaccaaa aaatctaact
     1021 tttggaggtc ggtatttttt atggctaagt atacttttga acttaaatta aagatcgttc
     1081 atgattactt ggatggtaaa ggcggaagtg attatctggc aaagaaatat tccattaagg
     1141 ctccatcaca agtgaagcgc tggatcaatg cctatcaaga atttggtgaa gagggtctag
     1201 taagaaagcg acaaaagaaa aaatattctg ttcaatttaa gctggatgcg atagagttat
     1261 atctaacaag tgagctgtcc tatcgtgaag ttgccaacac acttaacatg aacaacccta
     1321 atttgatcgc tagttggatg cgtcaattcc gtgagggtgg gatcgatgga ctctcaaaaa
     1381 cgaaaggatg tccacctatc ttgtctaaga aaaatgaacc caaaaataag aaaaaatcta
     1441 caactaaagc aacgtctaaa gaacgtgaac gcatcgagga gttggagaaa cgagtccgtt
     1501 cccttcaaat tgaaaatgcc tttttaaaag aattgaggaa gctgcgaaaa caggaagccc
     1561 aacaacgacg aatgaagcaa tcgcacgaat catcgcaagc ctccgaggac cgtttaaatt
     1621 agttgagctt ctagaaactt taaagttccc taaggcaatc tttatgtact ggcaaaaacg
     1681 attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaag ttcgggagaa
     1741 gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg gtttctctat
     1801 caataaaaag aaagttcagc gcctgatcaa gaagctagga ataaaagtaa cgacatacac
     1861 acgtaaatca cggcgctaca attcttatcg aggacaagtc ggagcagtag ccaaaaatcg
     1921 gatccatcgt cgtttctaca ccagtatctg ccatcagaaa atcacgacgg atacaactga
     1981 atttaaatac catgaagtgg atgccaaagg cattatccgt caaaagaaat tgtatctaga
     2041 tccttttatg gatatgttca atagtgagat tttatcctat cgtatctctg aaaagcccaa
     2101 tgcattagcg gtaatggaag gtttggagga agctatccag accactaacg attgtcccta
     2161 tcgtcgtacg tttcactcag atcaaggttg ggcgtatcaa atgaaagctt atagaaacaa
     2221 attgaaggag tacaaaatct ttcaaagcat gtctcggaaa gggaactgct tagataattc
     2281 acttatggag aatttttttg gcttattaaa acaagagatt tttcacggga aagtttataa
     2341 ctgtttcgag gaattaaaat cagccatcga tagctatatc tattactaca ataatgaacg
     2401 gataaaacag aaattaaact ggcaaagtct cgtccaattt cgtaaaacaa ccgtaacggt
     2461 tgtgtaaaaa tatcatttta gagcataaaa aaaaggagtg gatttctcca ctccaaaaaa
     2521 gtctaacttt tgggggtcac tacagtgtca cagtcctttt tttagtaaaa ggatacattt
     2581 cttccttgta tattccatgc gtgttcgtga gaaagcatat ataatttaat taacagatag
     2641 ttagggggag aaaaaatgat tttttcaatt acaatgaaac tggtgttcgg gttgatcggt
     2701 ttattattag tggtacgttt gcttgggaag aaatccatgt cagaaatcac accgtttgat
     2761 ttagtctata cgcttgtttt aggtgggatc ttagaagaat caacctatga tgacaatgtc
     2821 catgtagggc atgtcctatt tgccattgcg ctttgggcag taatgattta tggtatcgaa
     2881 cgtatcgtcc aaaaaaatga aaaggtcaat cgttgggtca aaggcgagcc atctgttcta
     2941 atcaaagacg gcgtcatcaa catgacagag ttgacaaata accatatcga gatggagcag
     3001 ttgcgagcaa tcttaagaca gcaagaatgc ttttttcttg aaaatgccaa acatgttatc
     3061 ctagagaatg ccggtcaaat gagtgtattg aaaaaatcag atgaagacaa agctttatcg
     3121 atcctgcttg tagatgaagg gcaaattcag cataaagtgc ttcagtccaa tcaattgact
     3181 gaagcatggc ttatggagaa cttgaagaaa gaaggctatg cagatgtaaa acagctgatt
     3241 tatgttgaat ggtcagaaga aaaagggctc tacatactaa ataaagaaga cgtcatgcat
     3301 gaatcttacc gaatagatgg atagatagac agaggttgtg aaatcagcgc cttggcttgt
     3361 tactcaaaac taagcgactt tttcacaacc tctgatatct aatatacgtt gttccagaag
     3421 ttgcttcttc gaaaaataag ccaaaattcc acaaaaattt aagaagcaat tttcgaaaaa
     3481 tcctttctta ttttttgaag ttgatcactt ctgtcacaac ctctcatgtc acaaaccatt
     3541 tttttgtttc ctttaactca atataccgta cgtcttccca caatatcggc tgatggtcaa
     3601 tcataaagta atcttcatag gcttcaccaa tgaagtgccc ttcgacctca tccaagagtc
     3661 ttccaaattc atcttttaaa ttgagctgaa tgattactgc tttacgagat aaaaaggctt
     3721 ctgtcagtat actatcgatt tcttgcaatg tcatttgttg tttaggaaga ggctgtttgc
     3781 gtgcttcttc ttcatttttt ttgataccgt tgactagttc ttccatcgca aaagcagtcc
     3841 cccattttaa tccaaatggc cgatctcgat actcgttata gtcaatgaat ttttttgctg
     3901 tccgtctcat cttttccacc ttctaatcca ctcataccac ctgcatgccc gccaacgaga
     3961 gaactacgtg cgatggcgcg tcctccttgc aagagactat gcgcatggat gattgattga
     4021 aagccgtatt tttttctaat cgtatcgaca acaaaatcaa ctttcgcttc atttatctgc
     4081 ttttcaggat cttcaaaaag gttaagctgt aaacctgtcg cgtatacgag gtcggaacag
     4141 ttcacgccta cacgccgaat atcctggtag tggtaatgct ggtggaaaat ggtcaataaa
     4201 gcatctgcta gatctctgct agcatttgtc gcttcgattt tcatttgctg gtgaaacccg
     4261 gtcttgccat ttggatcaag atagcctaaa gagtatccta cccataagct gactacttgt
     4321 gtttttgctg cagatcgtct caagcgagtg ccgacttggt cggccatttc tttgataacg
     4381 atctcgattt cttctcgtct tgtataatct ctatctagga tttgactatt tccgatactt
     4441 tttgattttg gtgtatattc ttgtcccaaa aaacttcgat cgatgcccca agcatgggca
     4501 taaagctgag taccgatgac ccctagcttg cttttcaagt gataataatc cgcgtgagct
     4561 aaatcataaa cagaatgtat acccatttgt ttcagacgtt ttgctgttcg tttgccgatt
     4621 ccccaaaagt cagtgatatc tgagattttc cataactttt ctggtacatc ttcataacgc
     4681 cattcagcaa acatttgctt tccgtctttt gagccattgt ctaaggctag tttagcaagt
     4741 agcgggttat cgccaattcc tatagtcgtg tagattccgg tctgctcaaa cacatcttta
     4801 tggatcagtt tagccatgtc gtacgcattt gttttaccaa agagtgccat gctatttgtt
     4861 acatcaagga agctttcatc gacggaatag acatgatggt tcgcttcgtc tgcatatttt
     4921 ttatagatag aattgatttc tctgttcttt tccatataat aggccatcct tggggcaaca
     4981 atatataaat cacttggata ggggaaggga agatctctgg cacggctgat attggtgatg
     5041 ccgtaagctt tttttgctgc aggactggaa gccaagatta atccgctacc ccgtgtttct
     5101 gtcgaatcgg acggatagga catgactact aatttagttg taaggggatc aagatccttt
     5161 tcaacgcact ctacggaggc gtaaaaagat ttgcagtcaa tacataaaat atctcttgaa
     5221 ggttcttttt cataaacaaa taccggattt tgattcgccc acataacaat cacctcttca
     5281 acacaattag aacacacgtt ctctttccgt gtcaataaaa aacagcaatt ttcaaatacg
     5341 agcaaaaggc tcacatttgg aaattgctga gaggttattt ttatctattt gtttttagct
     5401 gagatcgttg atttttttat caaacatgaa cagcttctgg atcgatcttt gttgtcccat
     5461 ttccaatgtg tgcaacataa aattccggag catagccgac aactttcatg tattcatcat
     5521 agacattgtt tttgaaggca ggtatatttt cttctttgac aagagcaatc gcacagccac
     5581 caaatccagc tcctgtcatt cgtgcaccta agacaccttc ttgtttttga gcagcatcta
     5641 ctagtgtatc taattcgatc ccagttactt cataatcata gcgaagagag tggtgagaat
     5701 catttaacag ttgaccaaat gcttctaagt tcccagcttc taattcagct tttgccttca
     5761 aagtccgctg attttcagaa acggcatggc gagctctgcg tatcaatgta tcatcgccga
     5821 tttggtcaat agttgattca aattcttctt catctaagtc tcctaaagaa gagataggca
     5881 aagttttttg caaacgtgcc aaggcttctt cgcattcgct acggcgttca ttgtattttg
     5941 aatctgccaa ttcccgccgc ttattcgtat tcatgatgac gattgcataa ccatctagtt
     6001 tcactggaac catttcgtat ttcagcgtat ttgtatctaa taagattgca tgatctagct
     6061 caccgaagcc gatggcaaac tgatccatga tacctgaatt gacaccgatg aattcatttt
     6121 ctactttttt acctgtttgg actagttcca agcgatctgt ttttaaatca aacagatctt
     6181 ctaatacaac acctactaat aattctaatg aagcgctact ggaaagtcct gcaccattag
     6241 ggattgttcc ttccactaat aactcaaacc catgatcgat tgagtgtcca gcttctttta
     6301 atttaaggac cattcctttt acgtaatttg cccagtttgc ttttttatca tattctagct
     6361 catctaaagt aaaactaatg acaccgatat cgtcaaagtt tgtggaataa actaatattt
     6421 tattatcgtc tcgaggtgct gctacacctt gcgttccata agtgatggaa gcggggaaga
     6481 catggcctcc attgtaatct gtgtgttcac caatcaaatt gatgcgtcct ggtgagaagt
     6541 attgtgtagt ttttttaggt ccgaatgttt cggcaaattt tgtgtttaag cttcttttga
     6601 tttccatgat gagttattcc tttcttgttt tgattttgaa ttctgtcact gtttcaaata
     6661 ctgatccggc ttttaaatga atcgaaccaa atgctgagaa gcgctctgca cctggagctg
     6721 tttgagtctc aaatgtgatg ccaccgtgat gtgccagttt tcggttgcgc atttctggtg
     6781 tttctgtgcc gaaatttgca gtgaaaataa ccacactaga agcatctgta gcaatatcta
     6841 cttggattct tttatcgggg ctagtcaagc gagcggcttc tttacccaag ccggtttctt
     6901 ttaagaaaaa tggatgatcg attccgtcaa caagattttt ttgatccaaa tcactagcaa
     6961 aaacggacgc taatttttta ggtatttgaa aatcgaaagc acttcctgca gcattttctt
     7021 ttacccctat aggaatacta tctgttcgca aaggagcata agcttcgcta ttgagccaaa
     7081 gctcatgttg atcgattgac tgagaagcat ctcccgtcaa gttgaagtaa acatgatttg
     7141 tcggattgaa taaagtatct tggtcactgg tgccgcgaga ggtcactcgc cagatattat
     7201 cttttgtcaa agtatagcgg atttccacat ccattgtacc tggaaaacca tgttctccgt
     7261 cgggacttgt agtagtaaaa atgacagaag cttcattttc gccattcaaa atgacataag
     7321 accatttttt taattcaaat ccaggagcac caccatgcag attatgtcca gtttttggat
     7381 ctgtggctaa ttggtaagtt tttccattga gagaaaagtg gccattttcg attctccctg
     7441 cggtgcgacc gatagaagca ccgatatacg gatctttttc gatatattct tcagcggtgt
     7501 caaatcccaa aactaattct cgatcatttg attttaaact gacgattcta gcacctaaat
     7561 cagagataga aagtgctagc ttgtttttgt tctccaaggt tatcaagtga taaccttggc
     7621 caaaatcttt ttctgtaatc ttcattttca agatcctttc ttaatgggaa ggaactactg
     7681 catcgtgaag ttcatcttcc attgtatcag ctggagccaa tttgttttcc aatttctcaa
     7741 cgatttgtgc atgttttttc tctgtcaatg taatcttcca agcaaaaata atagcggaga
     7801 aaatcatcaa agcagcagga gcatagaatg cataagcttt gaaagtcgtt acgccatgag
     7861 cagtgatatc agcagcagta gcattaccag tcatcccaga agcgatagcg acaaacccta
     7921 cgatcccgtt agacaaagca ccagcgattt tatctagtaa agggcgaatt gatagagtaa
     7981 cagcttcgtt acggatacca tttttccatt gaccatattc aacagagtct gtgatcgtca
     8041 tcaaagcgga taagaaaatc aattgttgag ggaagtagaa caatactagc ccgatgataa
     8101 cgattggcaa acttgaacct gcgatcgtaa agaaaaagta agaagaaatc atcattgcga
     8161 tccctccaag gtaaacgtag cgacgagaga tgacgcgtgt aagcattggg aatagtggaa
     8221 cagctactaa accaagtaag gcagcaacta gaccaacgat ccaaaattca gttggttggc
     8281 caagtacata tttgaagaag tagaataata cggcagttgt tgctacattg gcaatcgcat
     8341 aagcgatata agcaagggca agccacatca actgatcatt ttgagcaact gctttgaaca
     8401 catctttgat cccagctttt tcattttgct gacggatcgc agtatcgact tcttttgtgc
     8461 ccaaggcagt gataatcgca gtaccgcctg aaataacagc agcaatcaca ccgaaccaga
     8521 accagcctga agcgccttca atatgtttat gagtaacaag ataggtaaag aaagtaacga
     8581 tagggacaac gactagagta gtaccatttg cacctagaga agagccgaaa cgagcaaacg
     8641 tagcaaattt ttcacgttcc tttgtttccg tactcaaagc tggaatcatt gaccaaaaag
     8701 cgatatcctt aaatgaataa aaactatcca ataagatgaa aacgattgca aataatatga
     8761 taaataaagt cgtattatga gtagctaagc caaagaaatt ggtaaagatt gctaccaaga
     8821 gtacggaact aacaacgccg ccgataacta accatggttt gaatttaccg tattttgtac
     8881 gagtattatc gatgatgcca ccaatcaaag gatcaaaaat aatttcaact aagcggatac
     8941 ctacaactaa gcttgtaact gtagcaatca tttttgcatt cgttgcctta tctgttcctg
     9001 cgaacatctg gcttgtcacg aataccataa aataagtgct taatgtaaca tagaatgtgt
     9061 cgtgtccaaa agcgcctaaa gcgtaggaca cacgttggat cattttgctt ttcataatct
     9121 gtattcctct cctgttcaat atataatact aagtaaaccg gttacaacga ataacaaaga
     9181 tcttttaatg acttaaactt aacaagaaat cgcttccttt ttaatatatt tctgtgactt
     9241 ttatttaact ttttgttgca ttatactaaa ggagtaagga gggatagacg tggagcaatc
     9301 gattttttta aaaaaggaac atcaacaagt agaaaaagaa ttgtattttg atttttgtgg
     9361 tttctctaaa acgatgccgt ttcattcttt tggtcctgct attcgcaatg attacatcct
     9421 gcatgtcgtg atggaaggca aaggatctta tcatgtgaaa aatcaacaat tccaattgaa
     9481 aaaaggtgat ctgttcttga ttcgtccagg agactcgacg ttttacttat cagatggaga
     9541 agatccgtgg atgtattgct ggctttcatt tggtgggagt gctgcagaag aaatcattcg
     9601 gcagtcgctt ttcagagaag accaatacac gatggtctct tctgacatac aaaaatatat
     9661 cgacattatt ttagaatgca tgaaagatac ggaagaaacc ttggaaaatg aattacagtt
     9721 aacagcatta acctaccgtt ttttatcagt ccttttaaaa gatggcggaa aggtcagcgg
     9781 tggtgtacaa aaggttcatt cctctttagc tgttgaaaca gtcaaataca tcgaggcaca
     9841 ttatgctgaa aaattgtctg ttgaggaaat cgcccagcat ttagcggtca atcggagcca
     9901 tttgtcgcga gtattcaaaa atcatatggg taccagtatc aaggaatatt tgatcggggt
     9961 acggatcaat cgaggggcgt ttcttctttc tctgacggat gaatccgtgg aaagtattgc
    10021 ctatcaagta gggttcaata gtctagtcgt atttagccga atgttcaaaa agagtaccgg
    10081 agaaacggct acgaactatc gtaaacgtat gaaaaacgaa aattttaaga atattgcttt
    10141 ggataccttg aaaaaacagt tggaaaaaca aaccgcagtt tcttggacaa cctgattagt
    10201 ctggttaaaa tgacaagcat cggcaaaaga aacttgtggc ggttatgcta cactgtaaag
    10261 cgaagactaa aaaggaggaa aagggatgcg tacacttatc atcattagcc atccaacgat
    10321 cgaagaatct ttaaatcagc aatttttcaa agaagcttct gctggtttac cagctacttg
    10381 gcatcatttg gaatcaagtt atccagacgg aaaaatagat atagagactg aactacagct
    10441 cttggcccag catgatcgga tcattttcca gtttccgttt tactggtata gttctcctgc
    10501 tcatttaaaa atttggcaag ataatgtctt ggaacgagca gcacatgtat taaaagggaa
    10561 agaactagga atcgtattga caacaggtgt ggcggaaaaa gaataccaag ctggaggaaa
    10621 agaagaatat acgatttccg aatttttgag gccgtatcag cgaatcgcaa ataaattcca
    10681 tatgacctat cttccgccat tcgttttagc acaatttatg tatttatccc aggaaaaacg
    10741 ttgggagaag ctaattgctt atcagcagta tctctcattg gaaggcaaac catcgctgac
    10801 tcagcggatt gactggttta ttcaaagagt acaggaaaat cagaaaatgc aggaagaaga
    10861 atctgaaaaa caagcatata tcgtagaagc actgactgag gcaaaagagc agatcgaaga
    10921 cttgaccttt acactgcaag aaatgaaagg aacttcatta tgacaaatga ttggctgatc
    10981 caacaattag atgaattaag cgaaaaatca gaatatcaag accgtgcgct cttatatgaa
    11041 ataaagaaac tgatggctga gatagacgaa cgaacaatgc agctgcaagg agaaatcgac
    11101 ggacaatctt ggaatcatct caactggtga aattttctta taaataacaa agataaaaat
    11161 gtgcacagcg acaattattt gctacaataa gaatatgaag cgtttgataa cgctattata
    11221 cataaaaatt ggtggtgaaa aaaatgtatg atgaaaatga gtggggagaa aatgttcgga
    11281 tgacccgaag aagacagcaa aaacaagcag ctcgtaagcg gatgatcatg attggggcag
    11341 cagttgccgt gtttttgatc atagcaggag gagttgcggg tctatatttt catggaaaaa
    11401 attcgtcaga agcgaaaagt acgaccataa cggaaacaac gacaaagaaa acagagacga
    11461 aacaaacaag tgagtcatca actaaaacaa aagagacaca aacttctgaa gaaaaaacag
    11521 caaatggttt gccagagcaa cttcaagcaa tcgtaaatga ttattcgaaa aagatcgaag
    11581 aaaagacgcc tgcattgatt gaagaatacc agacagaaat tcagggaaat caagagggaa
    11641 tagctggttt atcagcagtt gctaaccaaa aagcacgtga gcttcaagca atctccgacg
    11701 aagggatcag aaaattacgt gcagcgtatc aggcagcaga aaataaagac ggtgtagact
    11761 tagatacttt gatcaatcaa ttatcggcta attatacgaa tcatgtcgct aagatttccg
    11821 atatttattt gcagactagc gcctctcttc aagcagagtc aacaagcaca caagacacaa
    11881 catcttctga ttctgagaca gcagagtcgg tagaagcttc tgaaatgcaa gctcgaacaa
    11941 cacaagacac aacagattcc tctgaaacta ataccaacag cgcgacttca caggctgcga
    12001 caaccgtagt tttggaagga gaaggtccaa atcaaatagc cgaacgaact ggcgtaccag
    12061 tagaaacgat cttgtcgctg aatgggatga gcatggagaa ttatttcctg actcccggtg
    12121 agacgctaaa attgaattga taaaagcaat aaaaaagaac gaagcttcca ctaaggtgaa
    12181 gcttcgttct ttttttattt tccggttctt ttttcgcgta caaagtaatg gatcgtcatg
    12241 taaagcaaga aaacgagtcc aataatcaat gagattctcg tttcaggatt gataaacatg
    12301 aaaatcaata cgatagctaa aaaagcaagt gtcacataat tcgtataagg agatagtggc
    12361 attttaaaag gatgatcatg cattttatct gcgtgcttct ttttgaagcg aatctcgctg
    12421 atcaaaatca ctacccaagg aaccatacca ggcaaaatac ttgagctgta tacgaaaacg
    12481 aaaatatttt ctgactggtt gatgaacatt ggtagaagat aatttaagat cagaccaatt
    12541 aagattccac ctgagattgc tacgacagga taaaaaggcg caccgtgacg agatagtttc
    12601 agaaattttt tagacatctt tccatttatg gcaagtgtat agaccatacg acttgcgctg
    12661 aaaatccctg aattgcaacc tgataatgcc gctgtaatca cgacaaaatt aatcagtcct
    12721 gcagcaaatg taatgccgac tttagaaaaa gtttgtacaa aaggtgaacc aatctcactc
    12781 aattgattcc acggatagat actcacaatg ataaaaatag caccaatata aaaaatcaag
    12841 attcgaccga tagtggactg gacagcttca atgatcgtct ttttaggatt ctctgcttca
    12901 ccagcagtca ttccgatcag ttcgattcct tgataggaag caacaacgat cgataatgca
    12961 aagaaaaatc ctttgacgcc tccagtgaag aatccaccat ttttccaaag atttgaaatc
    13021 cctactgcct gaccatgatt accaaatcca aataagatta ccccaagacc tgcaatgatc
    13081 atcaaaatga tcgttatgac tttgatgatc gaaaaccaaa attctaattc gccaaacgcc
    13141 ttgacagaaa taagatttgc cagcataaga aacaagacga caatgattcc gggaataatt
    13201 gccggcaaat caggccacca ataattcata taagatccta cagcaatcac ttcacttatt
    13261 cctacgacaa tatattgaaa aatattactc caagcggtca agtagccagc cacaggatgg
    13321 atgtactcgc tcgcgtaatt tgcaaaagaa ccagtcgaag gatcaacgta aagcatttcg
    13381 cctaaagcac gcataatcaa atacaaaaag aatcctgcaa tcgcataggc tcccatcact
    13441 gagggccctg tccaacgtat ggttgaggag gcacccataa acagaccgac accgatcgtt
    13501 ccacctagtg cgatcatttg gatatgacgg gcactaagac ttcgagttaa cttttcttct
    13561 tccatacaaa aactcctttc gttgttgagt caacaagaaa aaataatcaa acgtttcaac
    13621 acatgttttt cattattagg ttagccttat tgaactcttg atgtgattct acagaaaatt
    13681 ttcataaaat gcgaggaaaa ttcgaaaaaa actttttcat cattctactt gaaaatgctt
    13741 tccattttag ttaaactata tttaaataaa aagttaggta aacctaaaaa ggagtgtaag
    13801 atttggaacg tacggccatt gtaattcaag acctttcagc cgcttatcaa gggaaaactg
    13861 ttttgaaaac tatcaacttg acgataaatc cacaaaaaat taccggtatt atcggaccaa
    13921 acggtgcagg aaaatcaaca tttatgaaat ctttgctaga gttgatacca gctactactg
    13981 gtaaagtcac cttttctgga cagtcggtaa actcagtccg taaaaaaata gcctacgtgg
    14041 aacagcgaag tgaattggat ctttcctttc caattgatgt attaggcgtt gtacttttag
    14101 gaacatatcc atctttacga attggacaaa gacctgggaa aaaagaaaaa gaacgtgcaa
    14161 gacaagcttt gaaaaaagta gggatggaag aatatgccaa aagacagatc agcgaactat
    14221 cgggtggaca gcttcagaga gtttttattg caagagctct agcccaagga gcagaatgga
    14281 tctttttaga tgaaccattc gtagggattg atgcgttaag tgaacgaaag atctttgaca
    14341 tcttgcagga attgaagaat tcaggaaaaa cgattttgat cgtccatcat gatcttcata
    14401 aagtagaaga atatttcgat gaggttattc ttttaaataa acagctgatc gcttccggtc
    14461 cagtacaaga agtctttaca tcgaagaacc ttcaattggc ctatggtgag atcatcagac
    14521 atttagtaaa aggaggagag aaaaaatgat ccaatccttt attgatggat taatgaatta
    14581 tcaattcctg caatacgctt tggtcacttc catgcttgta ggacttgctt ctggagtcat
    14641 cggttctttt attattttac gagggatgtc tttgatggga gacgctatct ctcacgcagt
    14701 tcttccaggt gtagccattt cttatatgtt tggttttagt tacatctttg gagccactgc
    14761 ttttgggatg ctaacagcag cagtgattgg tttcgtgact cagcggagcc gattgaaaaa
    14821 tgatacagcg attggaattg tctttagctc cttttttgca ttagggatta tcttgatttc
    14881 atatgcccag agtgcgacag atttgtatca tattttattt gggaatgtct tagctgttag
    14941 ggaaagcgat ttgttgctga cagcactcgt tagtggaatc gtcttgatat ttgtcttttt
    15001 cttttataaa gaattgaaga tcacatcatt cgatccgaca atggcaaaag cttatgggct
    15061 gaacacatcg ctgatccatt atcttttgat gttctttttg acattagtgg ctgtagtcag
    15121 tttacagaca gtaggaacaa tcttggtgat tgccatgttg atcacaccag ccgccacggc
    15181 ttacttgcta acgaaccatt tactgaaaat gatcattaca gctgcaggaa tcggtatgct
    15241 aagtgcagtt gtcggtgtgt ttttcagtta tagttacaat tggccatcag gagctacgat
    15301 cgtgttagca tgtaccgcat tttttatcct tgctttttta ttttctccaa caaaaggaat
    15361 tttatttcag aaaggaagca gatagatgaa aacaaaaaaa tcactctttt taatcttggc
    15421 agttagtttc ttggtattag ccggttgcgg aaagcaggct tctgaccaag cggatgaagg
    15481 aagtaaagaa aagttgtcag tcgtggctac caattcgatc ttggcggaca tggcaaaaga
    15541 ggtcggaaca gatcaaatag atatccacag tatcgtacca gtcggaacag atccgcatga
    15601 atatgaagta ctaccagaag acatcaaaaa ggcaagcgat gcagatgtta ttttatacaa
    15661 cggtttgaat cttgaaacag gtaacagctg gttcgataac ttgatggaaa cggctaaaaa
    15721 agaagaagga aaagattatt ttgcagttag caaaaatgta gaacctcttt atttaactag
    15781 cggtgaagaa catacaaaag cagatcccca tgcttggcta gacctatcta acggaataaa
    15841 atatgtggag gaaatcgcac gtatattctc tgaaaaagat gcagaaaatg cgacactcta
    15901 taaaaaaaat gcagaagcat atgtggaaaa actaaaagaa ttagatacac aagcgaagga
    15961 aagttttgct tctatcgaag agaacaaaaa attattagta acaagtgaag gggcattcaa
    16021 gtatttttca cgagcatatg atttgccagc agcttatata tgggagatca atacagaaag
    16081 tcaaggtacg cctgatcaaa tgaaagcgat cattgatcag ataagagcaa aagaagtacc
    16141 ggttttattc gtggaaacca gtgtagactc aagaagcatg gaacgggtag caaaagaaac
    16201 gggtttgaaa atctatgata aactgttcac tgattccata gcaaaagaag gagaacaagg
    16261 agattcttac tatcagatga tgaaatggaa tattgaaacg atccatgaag gtttgtcaca
    16321 aacaaaagaa agctgaaaag tgcaaggtct agcttgagga gcagaccttg cattttttat
    16381 aagagatatg aaaaaaaatt atgctgtttt tcgtcgtaca gatacctttt ttgaagaaat
    16441 tccgttatag ttagagtaag gaaggaaggt gtggctatga attttacacg taaagataaa
    16501 tatacgatta aagaagaagt ctttcgattg atcagccagc aggaaaatac cgacaaggat
    16561 caagaccttc aatttcttga agcagaatta gctaaattat tcatagcaaa tcaaaatgag
    16621 acaacagaac cagatgaaga gctgtacata ggcaaatggt tgtttgatga atttcagcct
    16681 ttagatgaaa ataccggtat cggttatttt gtgttccttt ctccagtcgg cttcacacct
    16741 ggcgtattag ttaaacaaaa cgacgaaata accgtcaaag catggcctgc attattttca
    16801 gtcatcaaag cagaaggagt aatcaaaaag atccagtcgg cctattataa aagtcaattt
    16861 attgccaaca gcaaattaat gaatatgttt tataatcgtc tagtagaaat tgctaggcct
    16921 gggaaaatcc cgcaaacatt cacgtacaca cagcaaaaaa atctctatga agaagtattg
    16981 tattcccgcg atctgtctga gttttcgcca tgggaagagg taaggctcca tgaactgtta
    17041 agtacaaaac cagtttcatt gaattttttt aatgcattag ataacaaaag aaaattaatt
    17101 tcaacaaatt attgggaagt atttgctgca agaaaaaaat ggatggaaga aaagaagatt
    17161 gccgatcagc agatccagtt tgtccagctg aaaaaagaaa aactatatta tgggttatac
    17221 agccgttaac agcaagcgag gaagaccatg acattaaaag aagaaatcat acaagaaagc
    17281 aaacgattag gaattgacaa aatcgggttc accacggcgg aaccttttga ttcattaaaa
    17341 gaatcgttgg aagaacaaaa agcagctggc catacttctg gctttgaaca tccaaatatc
    17401 gatgagcgtc tttatccaga aatgacattt gatcagccga aatcgatcat ttccattgca
    17461 cttgcgtatc ctactaggat ccatgaagaa gtgcctaggg atgaaaaacg cgggcagttt
    17521 gctcgtgctt cttgggggat cgattaccat gatatactca gagaccgttt gaaccgattg
    17581 atcgaattca tccaaacaag agcagaaaaa tggcaaaaag aagaggaatg gcgtctagct
    17641 ccgcaagtag atactggaga actgatcgat gtcgctgtag cacagcgagc tggtttagga
    17701 tttattggac gaaacggatt gttgatcact gaagagttcg gttcatttgt ctatcttggt
    17761 gagatcgtta ctaatattgt atttgagcct gataaaccag gagaatttgg ttgcggtgac
    17821 tgtatgcgct gcgtgactgc ttgtccgaca aaagcattgc ttggtgacgg aagaatgaat
    17881 gcccagcgat gtttgtctta tcaaactcag acaaaaggga tgatgccgga agaatatcgt
    17941 aaaaaaatgc gaaatgtcat ttatggatgt gatatctgcc agttagtctg cccttacaat
    18001 caaggaaaag attttcattt tcatgaagag atggaaccaa aagtagacga aatatatcca
    18061 aaattaaagc caatgctttc gatgtctaat aaagacttca aacagcaatt tgggcattta
    18121 gctggttcat ggcgtgggaa gaaacctttg cagagaaacg cattgattgc tttagctaat
    18181 ctaggtgatc gaacggcatt gcctgagatt tcagaatgtt taacagatgt ccgccctgtc
    18241 atcagaggaa cagcagcttg ggctattgga cgtctaggaa caaaagaacc ggaaaaatgg
    18301 cttgatgtat tagatgaaat attagctaaa gaaacagaag aagtcgttat aaacgaattg
    18361 gaacatgccc agaaattatt gcggagaaag ttaaaataaa aaaatagtta acattattca
    18421 aagtttttgt cc
//
LOCUS       DAXWLG010000044        17965 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXWLG010000044 DAXWLG010000000
VERSION     DAXWLG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.044"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            214..498
                     /locus_tag="ACGE4T_001199"
     CDS             214..498
                     /locus_tag="ACGE4T_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFF8386488.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="ACGE4T_001200"
     CDS             complement(779..1198)
                     /locus_tag="ACGE4T_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFF8386489.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="ACGE4T_001201"
     CDS             complement(1200..1784)
                     /locus_tag="ACGE4T_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFF8386490.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACGE4T_001202"
     CDS             complement(1831..3306)
                     /locus_tag="ACGE4T_001202"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFF8386491.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACGE4T_001203"
     CDS             3487..3798
                     /locus_tag="ACGE4T_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386492.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3914..6250)
                     /locus_tag="ACGE4T_001204"
     CDS             complement(3914..6250)
                     /locus_tag="ACGE4T_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFF8386493.1"
                     /translation="MLQLKDIKKYYKVGEPTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6392..7060
                     /locus_tag="ACGE4T_001205"
     CDS             6392..7060
                     /locus_tag="ACGE4T_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8386494.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7063..8106
                     /locus_tag="ACGE4T_001206"
     CDS             7063..8106
                     /locus_tag="ACGE4T_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8386495.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8343..9224)
                     /locus_tag="ACGE4T_001207"
     CDS             complement(8343..9224)
                     /locus_tag="ACGE4T_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8386496.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9383..10111
                     /locus_tag="ACGE4T_001208"
     CDS             9383..10111
                     /locus_tag="ACGE4T_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFF8386497.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10267..10584
                     /locus_tag="ACGE4T_001209"
     CDS             10267..10584
                     /locus_tag="ACGE4T_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8386498.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10577..11185
                     /locus_tag="ACGE4T_001210"
     CDS             10577..11185
                     /locus_tag="ACGE4T_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFF8386499.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11240..12031
                     /locus_tag="ACGE4T_001211"
     CDS             11240..12031
                     /locus_tag="ACGE4T_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFF8386500.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12169..12657
                     /locus_tag="ACGE4T_001212"
     CDS             12169..12657
                     /locus_tag="ACGE4T_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFF8386501.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12708..13580
                     /locus_tag="ACGE4T_001213"
     CDS             12708..13580
                     /locus_tag="ACGE4T_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8386502.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13756..14118
                     /locus_tag="ACGE4T_001214"
     CDS             13756..14118
                     /locus_tag="ACGE4T_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8386503.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14127..14879
                     /locus_tag="ACGE4T_001215"
     CDS             14127..14879
                     /locus_tag="ACGE4T_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386504.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14893..16695
                     /locus_tag="ACGE4T_001216"
     CDS             14893..16695
                     /locus_tag="ACGE4T_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386505.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16972..>17965
                     /locus_tag="ACGE4T_001217"
     CDS             16972..>17965
                     /locus_tag="ACGE4T_001217"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8386506.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPN"
BASE COUNT         5625 a         3123 c         3715 g         5502 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaaacagc gcgaaacaag gaacatatta
     3841 cttcctcgtt tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt
     3901 ggttgtcaca accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc
     3961 gtgcaggaag atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg
     4021 catggacagg atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat
     4081 tgatagggaa cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta
     4141 aaatacacgt ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt
     4201 ttagtacacc gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca
     4261 tgatcatact tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat
     4321 ccatcaatcc gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat
     4381 cttcatctga tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt
     4441 caaaattgtt cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa
     4501 gcaatgtttg tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt
     4561 ctctttgagc tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt
     4621 gatcgctata agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta
     4681 ggatccccga gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat
     4741 tagggataaa attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga
     4801 atgttgtacc aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa
     4861 gattcttcaa tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg
     4921 ttgctgaggc tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt
     4981 tgctagtgtc aagctgtgta gggaaacttg acactcccac acctgtcatt gctgacatcg
     5041 tactagataa agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta
     5101 ctttgccatt tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat
     5161 tacttaagtt aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg
     5221 tgtgctgtgc tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat
     5281 ccgctttgct tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa
     5341 tagtaatcgg aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc
     5401 cagaagaaag ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg
     5461 tcaagaaagt acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc
     5521 aaaaactcat ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag
     5581 gatgggaatc tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat
     5641 gggccagttc aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga
     5701 tcaattccat gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca
     5761 aaaggatatc tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac
     5821 cagataactg atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat
     5881 cttctgcccg ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa
     5941 cgttttcaat aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag
     6001 agttgtttcg gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga
     6061 ttaccatatc accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt
     6121 taccagaacc actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca
     6181 caccatccaa tgctttggtg gtgggttcgc cgactttata atattttttt atatctttta
     6241 attgaagcat aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca
     6301 gaacaatgat cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc
     6361 tagaaatttt tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga
     6421 tccgcaatta cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac
     6481 cgcattgaat ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat
     6541 tacagatatc atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac
     6601 cagatttgac acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg
     6661 cgggttcaaa ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat
     6721 cctacgtgtg gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat
     6781 cggacaaaca aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat
     6841 tctgcctcaa aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt
     6901 cactcgccaa cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac
     6961 gatcgatgta catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat
     7021 catcacagta cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt
     7081 tcacaattat ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg
     7141 tgttttctcg gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct
     7201 ggaaaaccaa cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca
     7261 ggaatatcga tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat
     7321 atgagtctgg tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca
     7381 gaagtacagc aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag
     7441 acactgagga atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca
     7501 atccaaggat atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata
     7561 tttttatatc ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta
     7621 aaattgaaca aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat
     7681 gaacaaatca gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa
     7741 ttggatatcg aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg
     7801 tggctgaata tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc
     7861 aaactatttg agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg
     7921 aatgaagaga ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa
     7981 atttctggga atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga
     8041 cgtattgatt atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa
     8101 caataggaaa aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt
     8161 cgtttttttg tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt
     8221 cttattgaac tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag
     8281 agagcgaaag tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg
     8341 gcttagtttt cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca
     8401 ccgactttac gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca
     8461 gtattttctt ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca
     8521 tttgcaatca tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca
     8581 aatccagctt cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg
     8641 agcaaaaagt tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga
     8701 tgatttttag gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa
     8761 cttggtgggg cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca
     8821 ttgtaagtta attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt
     8881 ttcaaaaact cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag
     8941 atatttaatg aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg
     9001 tattgttcta cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg
     9061 atttttctat gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc
     9121 tgtttagaaa tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt
     9181 gtttcggcta aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt
     9241 attccaataa agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta
     9301 caatctttct tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt
     9361 agaaaaagag aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt
     9421 gtttggcgga ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca
     9481 gctgacgatg gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat
     9541 gaaaaatatg ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt
     9601 tcatttaggt aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat
     9661 cttccctagt gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt
     9721 cattgttcta ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac
     9781 aggagacagt acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt
     9841 tgcgtgtaat ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta
     9901 tattttgttc ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga
     9961 ttttaaagct tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa
    10021 aatgttccca gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat
    10081 gtggacaaat tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct
    10141 gactaaaaag gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa
    10201 ctatattata tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt
    10261 gacgtaatgc acatccagat tccaactgtt cttcttgatg gtactgtgct agcagcatta
    10321 agcaaggaag atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc
    10381 agtgaatcta cactatatcc ggttttgaga agattgaaaa aagaaggata tcttgagacc
    10441 tatgatgaac cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt
    10501 cgtcatttag aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta
    10561 ttggaggaga gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat
    10621 tcctgaagac gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg
    10681 aaaaacatta gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt
    10741 aaaaatcagt tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa
    10801 aagacaaatt cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat
    10861 tccactagca ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct
    10921 catttttagt atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat
    10981 ctcaggaatc agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg
    11041 tctgttcgcc acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg
    11101 gcttttttat gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag
    11161 agatctgcat tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt
    11221 gattaccggt attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt
    11281 aggcgctcaa aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga
    11341 gacaaaaaaa atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc
    11401 ggtcatacgc ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga
    11461 aataaaggag aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat
    11521 cattagttta gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt
    11581 atctgaattg acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa
    11641 aaaagtgaat actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac
    11701 aataactgcg aaagatgaat taggctcact tcgattagat gaggccaatg tggataactt
    11761 gatcgtcaca ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg
    11821 aaaagtcatc ctaaacggtg gcaatttatc tttgcatgag atggaaaaca gtggctatac
    11881 tatccaaacg tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt
    11941 gttagaacaa ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac
    12001 gacggatcaa gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa
    12061 aaacagcaaa gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag
    12121 taaaataaga aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa
    12181 gaaggtcaaa gagaaaagtt aaaaacagaa atggaaagat tacatactta tatcactcaa
    12241 ctaagccaaa ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat
    12301 tcagaaggtc gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg
    12361 aagaaagagc tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat
    12421 gggaagaagg aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg
    12481 atggatctag aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca
    12541 aaattggatt atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact
    12601 gaagacaaag agttagaact agaaggtgta atggcgcgta ttcgaaaaaa aaattaaaaa
    12661 agtaacatta gatatttatt gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa
    12721 gagcatttta tatcaatggt tatgcaggga ttgtcggatt ggtcgtatta gctttagttg
    12781 gattgttttt cttctattta ggtatgtggc aagcgaaagt ttgggcactt tttttaagta
    12841 tttttctttg gttgatcacg ttattgttgt taagttcggc aactgtagtc agtcctaatc
    12901 aagcaaaagt aattttgttt tttggacaat atttaggtac gattcgagaa aatggctttt
    12961 tccttacgat acctttggca caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata
    13021 gttctgtttt aaaagtcaat gatttggatg gaaaccccat cgaaatctca gcagtagtag
    13081 tctttaaagt cgttgataca gcaaaggcat tattcgatgt agcgtattat caagattttg
    13141 tagaaatcca aagtgagaca gccattcggc atatcgctag ccaatacccg tatgatacat
    13201 tcaatgagaa tgatttgacg cttcgaggaa acacgacggc agtgtctgac gagttgcaaa
    13261 aagagctgca agagcgttta gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc
    13321 atttagccta tgcaacagaa atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca
    13381 ttttgtctgc tcgacaaacc attgttgaag gagctgtcac aatcactcaa atggccttag
    13441 agcaaatcga agatggacaa gaaatcaatt tcaccgatga tcggaaagtc caattgatca
    13501 ataatttact tgtctcaatc attacagaca aaggaacgca acctgttatc aatacaggcg
    13561 acatgaaaga ccgtaagtga catacttttt gtaagatagg aagaaggagt ttaggacgga
    13621 ttattgtctt gaactctttt tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa
    13681 tgatagttga ttcgaaggca gaattgttcg cttttttcta taatcagtat ataaagtaag
    13741 agagaagggg aaaaagtgaa aaaacttctg atcggtctac ttgttttcat cgttgtggta
    13801 cttgcagggg tgaaaatcgc agattacgta gttatgggtg gagaagaata ctatgtacaa
    13861 attacgacag atggtacaaa aacagtttca aaatcagact ctggacaaga aacagtcaac
    13921 tatagatata tcctgcctgg ctatacgaaa gagggtaagg aaaaagaact ggacttcaag
    13981 ggacaattga ctcgaccgct tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat
    14041 aaaggtgtca cttcgtatga agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa
    14101 aaactaatta aagggtaggg aaagaaatga acaaaatagt cgaagtacag aatgtaacaa
    14161 aagtatacgg aaaaaacaat gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg
    14221 tagaaaaagg cgaatttatt gggatcatgg gcgcttctgg ttctggtaag tcgacacttt
    14281 tgaatatttt atccacatta gataaaccaa cagatgggca tatccgaatc aatcaaaaag
    14341 atgtgaccac attaaaagga aatcaactag cagattttcg tgcgaatgag attggcttta
    14401 ttttccaaga tttcaattta ttagaaagct tgacagcaca agaaaatatc gctgttcctc
    14461 tttctttaca aggcgtcaga ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac
    14521 gtttatccat ctctcatatt ttggaaagct atccttctga gatttccggt ggacaaaaac
    14581 aacgggtcgc cgccgcacgt gcattgatca cacaaccaac gattctatta ggggatgagc
    14641 ctacaggtgc gctggattcc aaaagtgccc gggatctttt agatacgatg gacgaattga
    14701 atacaaaaga tcatgtatcg attcttcttg tgacgcatga tccgttttct gcaagttatt
    14761 gccaacgtat tttattcatc aaggatggcg gtatccatca agaagtgaaa cgtggagatc
    14821 aaagccgtga ttccttctat cgagaaatac tatctattct tggaaacttg gaacagtagg
    14881 gggagattga ctatgctttg gaaattatca cttactggga tcaagagccg tttaagagac
    14941 tacttggtgt tattctcagg attagttatg gcatctgcta ttttttacat gtttgaatca
    15001 atggcaagca acgaagcttt tttgaaaagt aatacgatcg ttgcggctac agttatcatt
    15061 ttccgattcg gttctgtttt actagggatc atcacattcg tttatatttt atatgcaaat
    15121 tcctttttga tgacgatgcg gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa
    15181 ggcagaaaaa tcgcacagat gattttttct gaaacattct tagtagggat cgcagctact
    15241 ttagtgggtt cagctgtcgg tgtaggattg acaagtatcg tccaccgatt attagtggat
    15301 cagttagatc tcaatatttc gcattttaca ccttttagta ttcacgggtt gttgatcact
    15361 gttttgtttt ttgctgtttt gtttttatta gctgctgttg tcaatgcttt ttcgattgtc
    15421 aaaaaaccga ttttagcatt gattcgtgcg gatcagacac caacaagaat caagcagcat
    15481 aaattcttgt ttttccttga agtagtactg ggaattgctt ttctaggctg cggttactat
    15541 atgatgaaac acgtggggac ctatcaagtc tttggtatag gggtagcctt agttacgatc
    15601 gtattaggaa cttatttcat tttccattct gtgatcattt tctttttatc attactaaag
    15661 aaatcagaac agatttcatt gaaaaaattg aacaatttca cattgtcaca gttaaacttt
    15721 cgtattcgtg aatatacaca aatgctctca atggtcgcta tgctgtttgc cttggcactg
    15781 ggcgctttga cggtaggatt aggttttaaa aatgaaattg cagacttgac agaaaacaat
    15841 tctggatatg atctagtgat gaataatcct cgggcagaag atcagcaaaa aatcaaagaa
    15901 ctttcaccaa cactttctgc ttcttatact caaaaagaag atgcgggaac gatttactat
    15961 aatgcaagtg aatttgcggc tcaaccattg atcatgataa aacatgaaga tggaacaccg
    16021 ccgaagatca cgaaagaaaa agttcctgct aaaaaaatga atgaactgac tgtacaagag
    16081 gaacttcgaa gctatgaatt gccggaacag caagaaaaag aaatcaaggt agtcagtcaa
    16141 tcagaatttg atcaactgaa cttgccacaa tccactttac agctagttca ggtaaaagac
    16201 ttccaagcaa acctgaaacc aatcgaaaca cttgttgctc aaaataaaat gaacaatcca
    16261 tctttgcaaa aagtagagta cagcaaccag aaatatgaaa tgtatgaagt gtataatggt
    16321 ctattctctg gttttgaatt tatgggattc ttcctaggtt tagctttctt gaccatgctt
    16381 gctagctgtt tgatgttcaa gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa
    16441 atgcttgaaa agataggaac acgacgcggg ttgcttaaac aatcgatccg tagagaagta
    16501 ggcgtgttat tcttagcacc aggaatctta ggtgtagtcc atgtcttatt cggattacaa
    16561 atgtttggtc tattcatgca aaatccatac aaagatattt gggttccgtt tacgatattc
    16621 ttcgtattgt atttcatcta ctatgtactg acgacatggt tatatactgg aatcgtattg
    16681 aaaaagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata
    16741 gctcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    16801 tttgttcgga aaaagaggtt gtgacaatat ttttgtcaca accttttttt cacggtcttt
    16861 tattagaata tagtaaattt tttctcgtta ctcacggaat aagctgttaa gaaaacggtt
    16921 tccatgtata ctggaatcag tgcaaatgaa agaaaagagg gtgcaaggaa catggaaatc
    16981 aatgaacaaa attttggcga aaactatcac ttgattacga tcacgaatca aaatgggaca
    17041 tctttgtctg tcagtgatct tggggcacgg gttgttcatt ttcaaacaat aatcgataat
    17101 gaaaaacgag agttgatctt aggttttgat gaagcaaaag aatatctaga aaaagaccct
    17161 tatatcggtg cttcaatcgg acgtatggct ggacggatta cgaatggcac attcaaatta
    17221 gatgatgaaa cgttcgaagt aaaaacagac ccggacactg gtcattgttt acatggtggt
    17281 gcacctagct ttgaagccaa gcagtggcat tatacagtca tcaatggtga aaacgaagca
    17341 tcggtcattt tctatcagac tagcccagat atggaaaacg gatttcctgg aactttagat
    17401 gtagaagtac ggtatacgct gacgaacgat gatacatggc gagtgacggt ccaagggatc
    17461 agtgaccgca aaacagtatt caatcctaca aatcatgtct atttcaattt aaccggtgat
    17521 ccagcacagt caatcgacga acatatactt tggttgaata gccgattatt tgctacgctc
    17581 aataaagata caacgcctac aggagaagta gtttcagtaa gtggcacagc atttgacttc
    17641 caacgtccta aaaaattagg tgaggtcttt gccagtgatt ttcgccaatc aatgatagtg
    17701 ggcggcatcg atcatccatt tttgttgaaa aaacaatctg gtttactaaa tgctgcggaa
    17761 ttgacaagcc cagatgaaaa agtatcgatc caagtaaaaa cagatgcttc aggaatcgtg
    17821 atttttacag caaattttgg agaagatgga ccggaaatgc gcgggaataa attagtaaat
    17881 catggcggta tcacatttga gactcaagaa ctgccaggag cggagcgact tgaaagtttt
    17941 ggagatgtca ctctggaacc taacc
//
LOCUS       DAXWLG010000045        17832 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXWLG010000045 DAXWLG010000000
VERSION     DAXWLG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.045"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            121..933
                     /locus_tag="ACGE4T_001218"
     CDS             121..933
                     /locus_tag="ACGE4T_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFF8386507.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLLRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            930..1730
                     /locus_tag="ACGE4T_001219"
     CDS             930..1730
                     /locus_tag="ACGE4T_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFF8386508.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1891..3159
                     /gene="rseP"
                     /locus_tag="ACGE4T_001220"
     CDS             1891..3159
                     /gene="rseP"
                     /locus_tag="ACGE4T_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFF8386509.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3227..4936
                     /locus_tag="ACGE4T_001221"
     CDS             3227..4936
                     /locus_tag="ACGE4T_001221"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFF8386510.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5148..9500
                     /locus_tag="ACGE4T_001222"
     CDS             5148..9500
                     /locus_tag="ACGE4T_001222"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFF8386511.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9644..10117
                     /gene="rimP"
                     /locus_tag="ACGE4T_001223"
     CDS             9644..10117
                     /gene="rimP"
                     /locus_tag="ACGE4T_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFF8386512.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10141..11316
                     /gene="nusA"
                     /locus_tag="ACGE4T_001224"
     CDS             10141..11316
                     /gene="nusA"
                     /locus_tag="ACGE4T_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFF8386513.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11338..11631
                     /locus_tag="ACGE4T_001225"
     CDS             11338..11631
                     /locus_tag="ACGE4T_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFF8386514.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11628..11939
                     /locus_tag="ACGE4T_001226"
     CDS             11628..11939
                     /locus_tag="ACGE4T_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFF8386515.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11952..14258
                     /gene="infB"
                     /locus_tag="ACGE4T_001227"
     CDS             11952..14258
                     /gene="infB"
                     /locus_tag="ACGE4T_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFF8386516.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14285..14632
                     /gene="rbfA"
                     /locus_tag="ACGE4T_001228"
     CDS             14285..14632
                     /gene="rbfA"
                     /locus_tag="ACGE4T_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFF8386517.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14740..15244)
                     /locus_tag="ACGE4T_001229"
                     /pseudo
     CDS             complement(14740..15244)
                     /locus_tag="ACGE4T_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15409..17772
                     /locus_tag="ACGE4T_001230"
     CDS             15409..17772
                     /locus_tag="ACGE4T_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFF8386518.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6095 a         3153 c         3920 g         4664 t
ORIGIN      
        1 aaaaaaagaa aaaattattc agtttttaaa taaaaaaaca atgatttcat aggaaaaaca
       61 gttagatatt gctataatac ttaagatagt gggaatcttt taagtatagg aggaaaaaga
      121 atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt
      181 aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg
      241 gcgcaaaacc gtcgtttgct acgagttgct ggccataaag aaggcatgga aacagtaaag
      301 aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg
      361 actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac
      421 ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt
      481 tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca
      541 aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc
      601 gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa
      661 atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa
      721 gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg
      781 cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg
      841 catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa
      901 aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg
      961 cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag
     1021 caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa
     1081 gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat
     1141 ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt atagttatga
     1201 ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc
     1261 ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag
     1321 aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat
     1381 atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa
     1441 cgattgaagg tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt ctttatttat
     1501 tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga
     1561 ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt
     1621 atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg
     1681 acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc
     1741 tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact
     1801 attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa
     1861 cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt
     1921 tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga
     1981 atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa
     2041 gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc
     2101 aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct
     2161 gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg
     2221 atggaattga gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga
     2281 gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac
     2341 ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag
     2401 cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc
     2461 atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag
     2521 atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt
     2581 gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca
     2641 ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca
     2701 ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg
     2761 aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg
     2821 gaaattttca aagccttagg atcactattt acaggtttca gtttagataa attaggcgga
     2881 cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt
     2941 agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc
     3001 ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt
     3061 caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg
     3121 ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta
     3181 taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa
     3241 aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat
     3301 cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt
     3361 agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg
     3421 agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg
     3481 ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat
     3541 ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata
     3601 taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc
     3661 tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc
     3721 aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt
     3781 caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa
     3841 agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac
     3901 tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc
     3961 gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga
     4021 agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc
     4081 ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact
     4141 gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata
     4201 tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata
     4261 tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt
     4321 ccactataaa aatgtgaatc caaaacgcga tttcactgtg ctttcttatg aagatcttcg
     4381 ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg
     4441 aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac
     4501 tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt
     4561 aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc
     4621 aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca
     4681 aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt
     4741 tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc
     4801 aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa
     4861 aactggcgaa atggttgaag tacgtaaaga agaattggcg aatactttac caatcctctt
     4921 gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt
     4981 tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat
     5041 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
     5101 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
     5161 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
     5221 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
     5281 aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
     5341 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
     5401 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
     5461 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
     5521 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
     5581 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
     5641 tggatgagca gcaagcggaa cgtgtattga aactttttga agaacgcaaa caagaacagg
     5701 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
     5761 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca gctaggacga aatattccag
     5821 ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg
     5881 aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt
     5941 tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag
     6001 atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc
     6061 aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac
     6121 atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata
     6181 gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggaa
     6241 aatggggaca ccgagctatc gccattacag accatggtgg tgcgcaagcc tttccggaag
     6301 cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg
     6361 tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat
     6421 acgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc
     6481 tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc
     6541 caggacatcc attgtcacga acaacggtag atctgactgg tatcacggat gaaatggttc
     6601 gagggtcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa
     6661 ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt
     6721 atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct agatatctgt
     6781 atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaagtttggt gttagcttgg
     6841 aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg
     6901 tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg
     6961 gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc
     7021 aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac
     7081 gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct
     7141 cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa
     7201 gaaaccgagc aaaattctat gattatattg aagttatgcc aaaagcggtc tatgcgcctt
     7261 tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg
     7321 tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga
     7381 acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt
     7441 tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat
     7501 ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg
     7561 cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag
     7621 gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc
     7681 cgttacctga gatcattcaa aaacgattag aaaaagagtt gaattctatt aatggtaatg
     7741 gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct
     7801 atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca
     7861 ctgaagttaa tccattagcc cctcattatt attgtccaga gtgccaatat tccgagtttt
     7921 ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg
     7981 gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg
     8041 gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata
     8101 actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag
     8161 tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt
     8221 ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg
     8281 gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc
     8341 cgatccaata tccagcagac gatcaaaatt cagaatggaa aacgactcat tttgatttcc
     8401 actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta
     8461 ttcggatgct tcaagattta tcagggatcg acccacaaac gatccctacg gatgacccgg
     8521 aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt
     8581 ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga gggatgttag
     8641 aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta
     8701 cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg
     8761 aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg
     8821 ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg
     8881 aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg
     8941 attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga
     9001 tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact
     9061 tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga
     9121 aacaagcaat gaaagaaatc acggacaagg gattagatgc ttccgtaaaa gaaaagaatc
     9181 aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga
     9241 ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac
     9301 catttagagc tgtcccaagt ttgggaacca acgtggcgaa acaaatcgtt gaagcaagaa
     9361 aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat
     9421 tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat
     9481 cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta
     9541 tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct
     9601 ttttaatgga attacgcact taatttttaa ggaggcgaaa cttttgagta gcgtcgttga
     9661 aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac ttgtcgaagt
     9721 cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca aagaaggcgg
     9781 tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc
     9841 agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg gagcagagcg
     9901 gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc atgtctcctt
     9961 ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat ttgatgctga
    10021 acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa
    10081 aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa tcagaaaaaa
    10141 atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa
    10201 gaaatcgtga tcgatgcttt agaagcagca ttggtttctg catacaaacg tcactatggc
    10261 caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg
    10321 gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca
    10381 cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa
    10441 gattttggac ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa
    10501 gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga
    10561 atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt
    10621 ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt
    10681 tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat
    10741 ccagacctat tgcgtcgctt atttgaacaa gaagtaccag aagtctatga tggaatcgtt
    10801 gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat
    10861 gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca
    10921 atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct
    10981 gtattcatcg caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca
    11041 aatccaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa
    11101 cgcggacaaa atgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca
    11161 gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg
    11221 catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc
    11281 aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg
    11341 aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa
    11401 gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa
    11461 atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat
    11521 aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt
    11581 cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta
    11641 atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg
    11701 gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa
    11761 ccgatgcaag tgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc
    11821 cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg
    11881 tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg
    11941 aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta
    12001 gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg
    12061 cgattactag cagtgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac
    12121 aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta
    12181 aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg
    12241 gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg
    12301 gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta
    12361 acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc
    12421 gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac
    12481 cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag
    12541 atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg
    12601 gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg
    12661 actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct
    12721 tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagta gtaacgatta
    12781 tgggacacgt agaccacggg aaaacaacct tattggatac attgcgccat tcacgtgtaa
    12841 caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg
    12901 gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac
    12961 gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc
    13021 cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg
    13081 taaataaaat tgataaacca ggtgcaaatc ctcagcatgt tatgcaagaa ttaagtgagt
    13141 atgaattaat tcctgaagca tggggtggag aaacgatttt tgttgagatc tcagcgaaat
    13201 tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt
    13261 taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag
    13321 gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcacgtt ggtgacccaa
    13381 tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg
    13441 ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag
    13501 caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac
    13561 gcgcaaaacg tgcattattg gaaaaccgtg ctgccacaag ccgtgtgact ttggacaact
    13621 tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg
    13681 tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc
    13741 gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag
    13801 cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc
    13861 aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag
    13921 aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc
    13981 aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg
    14041 taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa
    14101 tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa
    14161 tgggttatga atgtggtgcg atgattgaaa aattcaatga catcaaagta gatgacgtaa
    14221 ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat
    14281 cattatggct aactaccgtg atcgccgagt cggtcaagaa atcttaaaag aagtcaatga
    14341 tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa
    14401 agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc
    14461 agataagaaa aaagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt
    14521 agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt
    14581 tgtctatggg aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaaa
    14641 tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt aagacgtatt
    14701 acctaagagt tccatgactt tagtctggta gcgtgtcagc taaccaaaaa agagaaaaag
    14761 aaaagtaaaa aattcgactt tcttttttct atagtatata aatttttgca tatagataaa
    14821 gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag tcaggtatag
    14881 ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg tcggtccgtt
    14941 gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc cgcttcgatt
    15001 tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc tgggatacca
    15061 attcacatat tgacaaagat aacagagact tttacataaa acggatcaat caggaaagga
    15121 attgctcgat ccagccccag ttttccaaca atgtgttaac tggcctagcg tcattcagca
    15181 aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata acgacaagtt
    15241 ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt
    15301 tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag gtttcaattg
    15361 ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat ggattattct
    15421 agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta tctttctgtt
    15481 gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc agaagatgtc
    15541 aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat ttatgtccat
    15601 ttgaaccgag tcatcaataa atatgatttg aacatgtttt atatcgaagg gccaggacac
    15661 ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga gatctatcca
    15721 gaagtaacgg aagatgagac aggaatgcaa aaattattca agcgattttc atttcctgga
    15781 ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg cggagagctt
    15841 ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt aatctctgct
    15901 gtcgtgattg gtgatggtga agcagaaacc ggtcctttag ctggaagctg gttctcaaat
    15961 gtctttatca atccagtgat agatggagct gttttaccga tccttcattt aaatggtgca
    16021 aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc aaattacttt
    16081 aacggacttg gctgggagcc tttctttatt gaaggaaatg atccagaaaa attaaatcct
    16141 gttatggctg aaaaaatgga tcaagcaatc gagaaaatca aatccatcca aaaagaagct
    16201 cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca
    16261 ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg aaccttccga
    16321 gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga tgctttactc
    16381 cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg tattttagaa
    16441 gaaatcagag aaattgcgcc tactggtgac caaagaatgg caaaaaatcc tatcaccaat
    16501 ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac tttgcaattt
    16561 gaaaaaccag gctccgttaa agcagaagat atgaccgaac ttggtaaatt cgttcgcgag
    16621 atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac aaaatcaaac
    16681 cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa
    16741 aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa
    16801 gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt tgcaagttac
    16861 gaatcattct tgcgagtggt tgactctatg ttgactcagc acttcaaatg gatacggaaa
    16921 tcacatgacc tttcttggcg aaaagattat ccttcgctga atctcattgc aagctcaaca
    16981 gtcttccagc aagatcacaa tggatatagc caccaagatc caggaatatt gacacatttg
    17041 gcagaaaaga aggcggagtt catccgtgaa tatctgcctg cggatgcaaa tacattgctc
    17101 gcagtcatgg ataaagcatt ccgctcgtca gaaaaaatca atttgatcat ctcttccaaa
    17161 catccgcgtg ctcaatttta ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg
    17221 aaaatcattg attgggcttc gacagcaaaa gaggaggaac ctgaattggt cattgctgct
    17281 gcaggaacgg aatctaactt agaagcatta gcagctgtaa ctttgctgct ggaagaattc
    17341 ccaaaactaa aaattcggtt catcaatgtc gtagatttat tgaaattacg tcatccaagt
    17401 caagatccgc gtgggttgag cgatgaagaa tttgatcaat attttacaaa agacaaaccg
    17461 atcttatttg ctttccacgg ttatgaaaca ttggtccgca cgattttctt tgatcgtcat
    17521 aaccatcatt taatgattca tggctacaaa gaaaatggag atatcacgac accatttgac
    17581 atgcgtgtag taaatgaact tgatcgttat catttggcaa aagacgcagc tttaaaaatc
    17641 aaaggaagtc aagcagaaga ttttgcggaa aaaatggatc agaaattaca agaacatcaa
    17701 aactatattc gtgaaaatgg tattgatctg ccagaagtat tggattggaa atggaaaaat
    17761 cttgaccaat aaaatgataa aatcgatccc gcaaaacaga attttttaca ttgtattatt
    17821 tgtcaaatta ag
//
LOCUS       DAXWLG010000046        17793 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXWLG010000046 DAXWLG010000000
VERSION     DAXWLG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.046"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..1692
                     /locus_tag="ACGE4T_001231"
     CDS             <1..1692
                     /locus_tag="ACGE4T_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386519.1"
                     /translation="LSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGADAP
                     FHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGSWFH
                     YQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTSWGT
                     CFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFFNQT
                     EKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGSYWL
                     HYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKNVPF
                     ISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLYQHL
                     EKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDNKYD
                     RYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTDYEV
                     TDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA"
     gene            complement(1794..2627)
                     /locus_tag="ACGE4T_001232"
     CDS             complement(1794..2627)
                     /locus_tag="ACGE4T_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFF8386520.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(2931..5096)
                     /locus_tag="ACGE4T_001233"
     CDS             complement(2931..5096)
                     /locus_tag="ACGE4T_001233"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFF8386521.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            5356..7650
                     /locus_tag="ACGE4T_001234"
     CDS             5356..7650
                     /locus_tag="ACGE4T_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFF8386522.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            7643..8308
                     /gene="pgmB"
                     /locus_tag="ACGE4T_001235"
     CDS             7643..8308
                     /gene="pgmB"
                     /locus_tag="ACGE4T_001235"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8386523.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            8301..9308
                     /locus_tag="ACGE4T_001236"
     CDS             8301..9308
                     /locus_tag="ACGE4T_001236"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8386524.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            9330..10349
                     /locus_tag="ACGE4T_001237"
     CDS             9330..10349
                     /locus_tag="ACGE4T_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8386525.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(10630..11997)
                     /gene="nhaC"
                     /locus_tag="ACGE4T_001238"
     CDS             complement(10630..11997)
                     /gene="nhaC"
                     /locus_tag="ACGE4T_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8386526.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(12106..13527)
                     /gene="tyrP"
                     /locus_tag="ACGE4T_001239"
     CDS             complement(12106..13527)
                     /gene="tyrP"
                     /locus_tag="ACGE4T_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFF8386527.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(13770..15647)
                     /gene="tdc"
                     /locus_tag="ACGE4T_001240"
     CDS             complement(13770..15647)
                     /gene="tdc"
                     /locus_tag="ACGE4T_001240"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8386528.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(15938..17194)
                     /gene="tyrS"
                     /locus_tag="ACGE4T_001241"
     CDS             complement(15938..17194)
                     /gene="tyrS"
                     /locus_tag="ACGE4T_001241"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFF8386529.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(17306..17511)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         5348 a         3772 c         3222 g         5451 t
ORIGIN      
        1 ttatctaaag tttgcaaagg agttcgacaa ttgaaaaaat atggcccatt tttagcaatt
       61 tttttcattg ctttggcatt tctcatgcct catttattca cacataaact aattttgggt
      121 gctgatgccc cattccatta caatcggttc tatgatgccg cgcaacaaat taaatatggg
      181 gatttccatt attttccttc cctttatgga ttccagcaaa gtggcaggat cgtgaatgcc
      241 gtttacggcc catttttcgc ttatttacaa ggattgatcg tgttgcttgc aggtagctgg
      301 tttcattatc agattctttc aaatttcatt ctttatctta tttcaggatt ttctatgctg
      361 attttactga gatatgtcaa tgttcgaaat tgggtaagtg taccagtagc cgttttattc
      421 atgagtacgt attctattca atactggtct atcaaccaag gattcactag ctgggggact
      481 tgtttttatc ctttatgtat gatccctatt gctgatttcg tccttcataa aaagttccct
      541 gttatcaaag tcgctgtctc agtgggactt atgacacaga tccatttact gtcttcgatc
      601 atgttagctt tgatgtatat ccctttttat ctttattact tttttaacca gactgaaaaa
      661 ggaaaagcac tgcttcaact gattaagtcg gtcttgcttt attttgttct gacagcaaat
      721 atttgggcag gattagctat tgtttacagt gggaataaga ttcttcctcc ctttgttaac
      781 agagaaatga gtcaaaaggc gatagatctg gatggcagtt attggctcca ttaccctaag
      841 gcctttccta tattgatatt atcaggtttt ttgcttttta ttttctttca acaaaagaac
      901 accttattcc aaaaaatcgt gttgtacaca agtcttgttt tcttttttct ttctactagc
      961 ttgattccat ggacgacatt gatcgaaaaa aatgtgcctt ttattagttt gatccagttt
     1021 ccatttcgat ttttcgtgcc atttacgcta ttgcttctac tttaccttgc cctttcactg
     1081 aaccagtggt ctgaacaaaa gtggttccgt cttttaagta tatttggcat tcttattttt
     1141 tcagcacaga cgatccagaa cttataccag catctggaaa agtgggaaaa tgaaacattt
     1201 gtcagtcgac atacttatct attcgatacg ccggaagaag caaggaaaac attcttcagc
     1261 aaagatatgt ctaccagctt gttcgttttc caaaaaacca cacctgacta tctgccaatc
     1321 tataaggaga caaaagacaa taaatacgac cggtataacg aagaaatact agaaaacgaa
     1381 acaactttta taaaaacaca tccaggcggt cttttgacaa tcaagtggaa aaacactggg
     1441 gacaaaactg ttcatatccc tgttatcgtc tacgatcgga ctgtgctaca gcaaaatgga
     1501 aaaacattga ctgattatga agtaacagat atcgggaccc caatcgtgaa gcagcaaaaa
     1561 ggaatcaacg agctcacgct tcattatcaa acgccaatct atttctattt cattctttct
     1621 cttactttga ttggctggtt taccttgcta tgtttattta tttatcatag atacaagctt
     1681 ttacatgctt aggcaaaagg gataaataca tccgatcaaa aaactctcca aacggtataa
     1741 tcgtttggag agttttattt taatcagttt ttttatgtta tatgttatat tttttattcg
     1801 ttcacatcta attcgatttc caccccgtag tgatcactga tgattggact atcgatccca
     1861 tcaaaaatcc gttgatattt tttaacctgc attccttcag ggacgaaaat aaaatctata
     1921 cgcaattttt ctgtattttg ttcccaacca tctattttct tttccacagt agctgctttt
     1981 cctttttcat tcgcgacaac atacgaatct tgaagaagaa ggcaactttc tcttacagta
     2041 taataacctt gagagtctac tggattattg aaatcaccta ggaaaaaaag tggtacgcga
     2101 gtttccaaaa agtagttttc caagtttttc cactcaaaat aaaagccact ttttttatct
     2161 gtccaccaag aaaagtggca gctcgagaca aaaatgtcct gatcatctat ctttgttttc
     2221 ccaatcaaga tcttgcgtgt ctgataatct tctttctttt gaaattggga gacggtaagg
     2281 acctgacttt ccaatgtttt tttcgtaagc agtgcatttc cttcttcata tttatcataa
     2341 ccgatgtggc tcatttccca actccaaaaa tactcttttc cgtgttcctt taaaagctga
     2401 actaaacggt aggcaaaatt atctttacgg ataggtactt gattctttac cggacagaac
     2461 ttcattagct tcgcaggttc tacttcttca gattccagta attggttgat ttcttgtaaa
     2521 gcaatcactt cataatcttc taacaggatc ttatctgcta attcttgcaa ttttttctct
     2581 ggttctggct caagccagct atgcgtgttg agtgtcatga atttcattgg ttttctcctt
     2641 gtcttcattt cgaaacaaaa gggttgtgac aaaatttttg tcacaacctc tccagcaaat
     2701 agtgttctcc tgctaactcc tcagtaataa gctcaatgga ccaggagcta ttttttgttt
     2761 ggctgttttt atcacttggt gcagggcagc tttttcacga cctctccact aaacagtatt
     2821 caaactgctg cttctcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg
     2881 tttggctgtt cttatgactt gatgcaggac agctttttca caacctctcc ttacatttta
     2941 atggagccga tcactttacc agctgtcact gtacctaatt gcttgatcac tacttcttct
     3001 actttatcgt ttgtaaatac gatcatgatc gttggatctt tttcttcgtt ttggatcaca
     3061 tcaagatcca tctcagctaa tttcgttcct gttcctactt cttggccttc tttgacagca
     3121 atctcaaaag ctggctgatt catttggact gtatcgattc ccatatggat caaaacttcg
     3181 ataccatctt tcgtttccag cccaattgca tgtttgctag ggaaaatact tgtcactttt
     3241 ccagcgatcg gcgcaacaac ttctcttgat gttggaataa cagcaaagcc gtcacccatc
     3301 attttttgtg agaataccgg atcttttact tcagataaag gaatcacttc tccagaagct
     3361 gcggaaacta gttcttgttc tatacctaag tattgcttac tacctgcttg tccatttcct
     3421 gtagaaacca tttctggctc tggaatctct gctcctgatt gcagcagatc ttcgatatct
     3481 gatttcaaga catctgcttt tggtccataa actgcttgga ctccattgtc tttgacgatc
     3541 aatcccatcg cccctgctcg tttccaggct tcttcagtcc ctactttttc tcgatcattt
     3601 acacttacgc gtaaccgagt catacaagca tcgacatctt tgatattttc ttttcctccc
     3661 agcaaatgaa tgatctggac cacttgctga tctgctgttc ctgttccgct gttagataca
     3721 acggtttctt cactgtcatt gtcgtaattt ccgttacgtc ccggtgtagc aaagttgaat
     3781 tttttgatca agaaatttgc taagaaatac gtaacaacac caaaagcgat cacacacaac
     3841 acgaagttga taagatctcc acctagaccc gctttgatag ctaacggtgt ccgagtcagc
     3901 aattcgatat tcccaaatga atggacccgc aatggcaaga tatctgccat cgcaaatgca
     3961 gccccttgga tcactgaata aatgacatat aatggcacag cagcaaacat aaacatgaac
     4021 tccaatggtt ctgttacacc tgttagaaag acagccaacg ctgctgaaaa atacattgat
     4081 ttatatcttg ctttcttatc aggatcgaca ttacgataca tcgcaaaagc cattcccatc
     4141 aagatacctg aagaaccaat catttgtcct actttgaaac gagccggcgt ccagttatgc
     4201 aacacaaact catattgcga catatcccct gcacctttaa gattcactaa atcagtggcc
     4261 caagccaacc ataacggatc ttgcccaaat acttgtgtcc ccgcctgtgc gcctgataaa
     4321 atctcatacg atccgccaag ctgtgtatag ttgataggaa tcgtcagcat atgatgcaat
     4381 ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa acggtgctag aatcggtgcg
     4441 ctttcttgtg attctgcaat ccataaacca aaattattga ttccagcctg tacgtttggc
     4501 cacaccacag caagaatcaa tgccacgatt gttgaccata agataacaac aaacggaaca
     4561 aagcgctttc cattgaaaaa tgacaaagca tccggcaatt ttctataatt atagtactta
     4621 ttataagcca ttgccccgac aaatccagcg atgatcccta cgaatactcc catattcaaa
     4681 gctggtgctt ccaatacact tgtaaaaaat cctttaacca tgatcttcgt accgaacaga
     4741 gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg tgacaccaaa gatagcccca
     4801 gtgatccgat tgattaaaat aaatgagatc ccagctgcaa atgctccgcc tgctctttct
     4861 tttgcccaac ttcctcctat tgctaaagca aataaaatat gaagattacc aataattgcc
     4921 caaccaatat tttcaattac tccgcccgtt gtgacaagcg gcgtccaatc aggattgatc
     4981 aagggaattg atttccctat actgatcatc aacccagctg ccggcataac tgctaccaca
     5041 accattaaag cctttccaaa tttttgccaa aactcaaaac taaatatctt tttcattccg
     5101 tttagctcct ttacgcaatc ggtttcgtta aacgtattat agcgtgcgtt ttcttttttg
     5161 taaatgcttt attttttatt tttctgtcta taccattcta taaaacctat aatacgtgtg
     5221 ttcccttcta taattttctc ctaaaaattt atttttttaa gcttttttct atgaaatatt
     5281 ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt atgaacgtat aaaacagctc
     5341 attaggagga gaaaaatgaa acagatcaaa cgactatttg ctattgatcc atggaaaatc
     5401 agcacgacac aattagataa aaaaaatctt cgactgcaag aatctttaac aagtattgga
     5461 aacggttata tggggatgcg cgggaacttt gaagaaactt actcagggga ccaccatcaa
     5521 ggtacttatt tggcgggtgt ctggtatccc gataagacac gtgtcggctg gtggaaaaat
     5581 ggttatccag aatactttgg aaaagtgatc aacgccttga actttatcgc gatagatatt
     5641 tcgatcaacg atcagctgat cgacttggct tctttggaac cagaagactt ttactgggaa
     5701 ttagatatga aaaatggtgt attatctcgt acattcacaa tcactacttc tactaataaa
     5761 gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa aagaatcagc ttatatctat
     5821 ttaactatag aaatgcttga aggtagcgga acagtcaaac tcgtatcaaa attggatgga
     5881 aatgttcaaa acgaagacag caactatgat gaacattttt ggaatgagat tgatcgtgga
     5941 actatagaag aaatcggcta tttgacgact aaaactattc caaacaactt tgggattgaa
     6001 caatttactg ttactgcagc aatgcgccat ttcgttaatg gaaaatcagt catccctgac
     6061 tatcacgaag atactttagc attacatggt tgcttgtctg cagaattatc tcaaggtgag
     6121 acatggtcag tggaaaaaga aattatcgtt cttactagta gagatattcc agaagcgcag
     6181 caagcagaaa aagctgtctc agaattaacg aaccagacaa acaattatat ccaagcaaaa
     6241 gaagagcaaa cagcggcttg ggcagaacga tggaagcttg ccgatgtggt catcgaagga
     6301 gacgaagaag cacaacaagg tattcgattc aatttgttcc aactattctc tacttactac
     6361 ggcgaagatg aacgattgaa tatcggcccc aaaggattca caggagaaaa atacggcgga
     6421 gccacttact gggatacaga agcgtatgct gtccctcttt atcttgcttt agccaaacct
     6481 gaagtaacta agaatctgct gaaatatcgg cataatcaac tgccgcaagc aatccacaac
     6541 gctcagcaac aaggattaaa aggcgcgctt tatcctatgg tcaccttcac tggtgtcgaa
     6601 tgccataacg agtgggaaat cacattcgag gagattcacc gaaatggcgc gattgcctat
     6661 gcaatctaca attatacgaa ttacaccggc gatacggaat atttgaaaaa tgaaggactg
     6721 gaagttttag cagaaatcgc acgcttctgg gcagaccgtg tccatttttc aaaacgagca
     6781 aataaataca tgatccacgg tgtgaccggc ccaaacgaat atgagaacaa tatcaataac
     6841 aattggtata caaatacgat cgcagcttgg gtcttgcgtt acacgcgaga aaattattta
     6901 acttatcaaa aagaagctgc tatcactatc agcgaagaag aactagccaa atggaaagat
     6961 atcgcagaga acatgtactt cccagttgac gaagaattag gaatctttgt ccagcatgac
     7021 acattcttag ataaagatct catgcctgta tccgatattc ccacaagcga tcttcctctg
     7081 aatcagcatt ggtcttggga caaaatcctt agatcttgct tcatcaagca ggcagatgta
     7141 ctacagggga tttatttctt caatgatact tttacaaaag aagaaaagca aaaaaacttt
     7201 gatttctatg aaccaatgac tgtccatgag tcttcccttt cgccaagtat ccattcgatt
     7261 ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa tgtaccagcg aacagctcgc
     7321 ttggatttgg ataattacaa taacgacaca gaagacggct tgcacatcac ttccatgact
     7381 ggtagctggt tgtcgattgt ccaaggattt gctcaaatga aaactgccca tgatcagttg
     7441 agattcgcac catttctgcc aaaaacttgg aaagcttatt ctttccatat caattatcgc
     7501 ggacgtttat tgttcatctc agtcacagat gaacaagtta ctttcgagtt attagaaggt
     7561 tctcctctag aaatttacgt ctatgaacag tcttattctt tagcggatcg tctagaaata
     7621 acgctaagaa aggaggaagc aaatgtttaa aggggtattg tttgatttag acggtgtcat
     7681 taccgatact gctgaattcc attatcgtgc ttggcgaaag cttggtcagg aaatcggcat
     7741 tacgatcgac cacacattca acgaacagct aaaaggcgtc agccgagaag actcactctc
     7801 tttgctttta gcttatggcg gaaaagaaca ctctttttca aaggaagaat ttgcagaatt
     7861 agcgaaaaga aaaaacgatt attatttaga gatgatccag acgatcgaac caaaagatgt
     7921 cttccccggg gtcgtgcctt tactggattc tttaaaagaa gaaaaaatca agattgctct
     7981 tgcttctgca agtaaaaacg ggccattcct acttgaaaaa atgggtctta caccttactt
     8041 tgatgctatt gccgacccgg cagaagcaac aaacggaaaa cctgcgccag atatcttcct
     8101 ccttgcagca aaagctgtcg gactaaaagc agaagaatgt atcgggatcg aagacgcgca
     8161 agccggtatc caagccatcc tctcaagtgg cgctcaacca attggcgtgg gacgtgctga
     8221 agaccttgga gaggagatcc cgcttgtccc tgatacgact tttcttacga tcgattactt
     8281 gaaagaaaag tggcatgacc atggataatc cagagatctc cgtcgtacca gttccagaga
     8341 ctgaaataaa gaaaatcaca ttgaagaacg actacttaga agtcgttctt ctcaattacg
     8401 gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg aaaatcagaa aatgttttat
     8461 tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag cttttttgga gcaactgtcg
     8521 gacctgtagc cggaaggatc cgggatgctt cttggggtga gcatagattg gagaaaaact
     8581 gcggatcaca ccatatccac ggtggcacga aaggctggtc tttccaattt tgggaagtag
     8641 aagttttcaa aactccatca tcaataggtg tggtctttta cttaaaagat gaattttcta
     8701 cctatccagg accaatcact gctacgatca cttaccgttt aacaaaaaac gaattagaga
     8761 tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa tccgaccaac catgcctact
     8821 tcaacctttc aggaaatgga acaagagaca tctatgaaca tcagttaacg gtctttttag
     8881 atggtgtgct tgaattagat caagaaaaac tgcctactgg aaattggaca aaaaaagaaa
     8941 aactgccaat cgactttaga aaatccccaa cactgcaaga gatccttgca tgttatccag
     9001 atggactaga tgatgtattt cttttgcatc atcctaggct ttccaaaact tccttacaat
     9061 tattcgagaa gcattctggt cgccaaatga ctatagcaac ttctaataaa agtatggtat
     9121 tattttctac gactggattc gaagctgatt tctctgtcaa tggaaaacag atgcattcaa
     9181 actacggatt agcaatcgaa ccgcaagaaa tccctgatat cgttcatttc cccaaatttg
     9241 gatcgatcaa cctccatcct ggtcaagaac ggatcagtca gacgatttat cggttctctg
     9301 cacaataaca agagaaaggt gtttttatca tggctattac cgtaaaagat gtggcgaaaa
     9361 aagccggtgt tgccacctcg accgtctctc gtgtcatcaa cgatcatcct agtatttccg
     9421 agacaacaaa gaagaaagtc cgaaaaatta tggatgaact aggttatgtt cctaacatga
     9481 ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg cgtaatctta ccccctcttg
     9541 attcaaaaga gcggatcggt aacccatttt atcttgagat catggaagca atcaacgaag
     9601 aagcgcggaa ttatgatatg actacagcaa tcgctactgc taaaaatttt gaattgctgt
     9661 tagagaacgt ccagcgtatg catttacaaa aacaagtaga tggttttatt ctagtgtatt
     9721 cagatagtca agatactgtc attgattatt tataccaaaa ccacatccct ttcactttga
     9781 tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt agataatgat aaccagcttc
     9841 taggtaaaca agcaacagaa tttttgatcc agaatggaca taagaatatc ctgtttgtga
     9901 caaacacgac tcatgaaagt ttgtatttcg aacgatattt cggttatcaa aaagcaatga
     9961 tgatggctgg tcttcctgcg ttatccagtg tagtactaga gcatccggag gattatctgt
    10021 cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt ggtggtcatt gacgatatct
    10081 tcgcccttcg cacgatgcag ttggcgcaaa tgtatggcta tgtcattcca gagacactct
    10141 ccgtcattag cttcaacaac tcgatcttct caacattaac acacccttac cttacaagta
    10201 tcgatatcga tatctccgaa ttaggacgtg tcgctatgca aaagctgcgc gaattgatcc
    10261 atgaagaatt gagcagtggc gtacgtttgg tcatccctca tcgcttgatc aaaagagaga
    10321 cagtcgttcc tttagaaaac ccatactaaa tagctgactc actaaatacg ctgaccattt
    10381 atcctatggt cagcttttta tgtaaaagaa gatactactt ctaacacatt gtttatctgt
    10441 aaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
    10501 acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttaaaaag
    10561 caattttctt gagtttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
    10621 ttccttcctt caatttttta cacgtcgacg attcaataaa aaaccgccta atattgtaaa
    10681 gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat
    10741 cgttcccata atgaatgtgc cgctgactcc ccacggtacg agagaattga cggtcgcacc
    10801 tgaatccgct agtgttcgtg tcaaataggt ttttgataag ccaacgtcat caaaagaacg
    10861 cttgaatgtt tctcctggaa gaatgatgga cagatactgt tctccaacga tcagattgat
    10921 tccgatacag ctcaaggcag taaacccgac taaacgagaa ggtcggtcca tcttttcttt
    10981 taattcttga acgatggtat caacgatcag atatttgatc agtaacccac ctaatgccaa
    11041 tgcaagtaaa atcaaactag cagaacctaa catactcatg atcccgcctc gagaaagcag
    11101 ttcatctagc tttggatcag gtgtatttgc gacataacca ttcatcaaga tgtctgctgc
    11161 ttttccgacc gtcaaacttt tctcatggat aaaagaaaga acgagagcag ctgtcgatcc
    11221 cgctagtaat gtagggatag ccggaatttt tttgattgcc cccccaaata aaataactag
    11281 tggaatcaat gtccaaggag agatccaaaa gctcgctttc atgttttgga tcatttcatt
    11341 gatactagct agatcggctg aatgactgtt atgtcctaat atcgtataaa aaatcaacga
    11401 aaggacgaat gctgggaaat ccgtataacg cataccagcg atatgttcga atagattaac
    11461 tcctccaata cccgctgcca agttggtcgt atctgataaa ggcgagcagt tattccctaa
    11521 aaaggcaccg gaaacgactg cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc
    11581 gatccccaaa aaagcaattc caatcgtcga aatcgttgta aatgaactgc cgacagtcac
    11641 tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt
    11701 aaatccataa accataatcg tagggatggt tccagataaa atccatgaag caactaagac
    11761 accaatcaaa agaaaaatca ggattggaat gatccctgga gtgattcctt tgatgatccc
    11821 gtcatggatc tcatcccatg tgaacttttt aaatctgcca taaaacaaca aacacatgaa
    11881 agctgcaagg atcgggatat gtggcgagag ctggaatccg atgatcagta ctcctaggag
    11941 tgccaacaac acgatcaata cagcgatact ttcttttaaa gaaatatttt tattcatcgt
    12001 cgatctccta tttcaaatga aaaggccctg ccatgaaaaa actattcact tggcaggacc
    12061 tcctactaaa tcagcgacaa aacgtcggct gaaagactta tttttttatt tttcttctaa
    12121 acttttttct acctcaatcc cccaagaatc tttcttgaag tgattgatga tcaatggaat
    12181 aacgaacatg accactgtga tagctgctac taataatgca tcactcaatg tcgctgtacc
    12241 taatgtagca aatacgacaa cgatgataga gaagatcagc atgcagctga ctacccaagc
    12301 taatccgttt ccgtttttac caattcggta aggacgttcg aattctggat gtttcttacg
    12361 cattttaagc aatgaaacag caatcaaaat gtacacgata caataaataa cagttgtagc
    12421 atttgtaagt gtcaagaata cagcacttac gtcatccatt acaccataaa gcaaagcaaa
    12481 taatgaaata acgattgatt gtgttagaac gacatttcta gatacgccgt atttgttttc
    12541 tttatggaat ccgaattttg gtggaaggaa tccttcacga gctacttgga tgattgtttt
    12601 tgatggtcca gtaacccaag ctgataattg taaaagcaca ccaataaata ccatgaagct
    12661 gaagatattt ccgatcactt ctggtaagcc taggatttga cagtcaatca agataggctg
    12721 cgtgatattt gctaattcca tttttccatc tggtacgaca tttgctacaa gcatcgcatt
    12781 gatgacattc aataaaacta atccgatcaa agcgataaat acgccttttg tataattttt
    12841 tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt tcgatcccaa caaaaataaa
    12901 tgtgatacca gctaagtatt tcaatgaatc aatatgttcc aagtcaggga aagcttttga
    12961 ccatgaaaag tcacctagat agccgtttga gactagtccg actttgaagg ctgccaaaac
    13021 acctaaaacg aacatgatga ctactgggat atatacccct agccatacac cgatgttccc
    13081 gccgactttt gccatatcga atttcaagtt caagatcgtg atgatccagt agataactaa
    13141 aatacaacct aaaacgaacc agtgattatt gccaagttct acattaccaa atgtgtttcc
    13201 taacaatggt cctaaagtag aagcgaccat taccatacct gggaacattt gcacccataa
    13261 tagccatgca acgacaaatc cccatttgct gcccagtgct gtttttaccc ataactgagg
    13321 accgccttct tgaggaagca tcgtggaaag ctcaccggaa accattgata tcggaccggc
    13381 aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc caaccgactg cggctaacgt
    13441 aggaatactc cgaaccgttg cacacagtgc cattgtcaga ccgataaagg tcccaagacc
    13501 cattttactt acttcagatt tactcaccgt tgacactctc cttgattttt tggataaaat
    13561 aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa ttcagcgaat atttgagggg
    13621 attctttgag tttattgctt gaagggtttc ttcaaagaca acctcaaaac aaagggatcg
    13681 aagagcttag gtctcccctt gctcttcaga tccaaacatg cgtcagaaac agttagtatt
    13741 caagctgttt tctaacatga ttaccttgct tattttgctt cgcttgccaa taattggtct
    13801 gcgtagattt tttccaattt ttcttgaaga gctgctttga ttttttcagc atattcttca
    13861 aagtgttctt ctttgttcat gtatggtgtc attacgctgg cacgcaatac agttactttt
    13921 ccggcacggt tccattcttc atctgagaat cctagttggt tcacaaattg taatgggctg
    13981 ttgccatagt ctgggatagc aaagtcagta tgtgaagtta agaactcatt accgtaaatg
    14041 cttcctttca cgtatgaaga ataatcatat acgtcatggt tcaatttatt cattgcaact
    14101 aagtcatcgt tgcctttttc tttgaataca tagtctacca tattgaaatc tggataagtt
    14161 aatggatgaa cttcgatttc tttgtctcct actttaaatg acaagtcatt caagaagttg
    14221 tagaaacgat gtgcaccttc gattgatgca cccattaatt taccatatcc tgtgacattc
    14281 aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct
    14341 tccaagatat aagccccaag taatgctggg atatctgcgc ctttttcaaa tacatatgtc
    14401 gcaaagtaag agataacgtc tctcatgcgg atatctttga tgacgatacc accggcagaa
    14461 tatggaacat agcccatttt atgtgggtca atcgttacag attctgcttc ttcgattgct
    14521 ttatatgcac tgtgtacttc ttctaagata taatctttgt tttctgtaaa gacattgtat
    14581 ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa
    14641 atcgcacggc cgtatccgcc ataagcagca tctacgtgta agtagaagta aataccgtct
    14701 ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac cgtcgattgc tccttcttct
    14761 gttgatccta caacaccgac tacacctagg attggtgttt tttcagctgc tagaccacga
    14821 acgatttttt caagttcgtt gatgtccatg cgatagttgt ggtcaactgg cactggaatc
    14881 acttggtcta aaccgatacc gatgatgtcg gcagctttta gccatgaata gtgtttagtt
    14941 tgtggaacca accattttcc taatttttct aaatgttttc cgctacgtgc tgagtgagct
    15001 ttgatttcgt cgattttttc tgggacgctg tctaacagat tcatgatttc ttctgttgac
    15061 aagttcatta attcccaatc gctttttcca gcaactagtt caggtgtcac ttctttcatt
    15121 gcaagcggca atgatttgat gttgcgggca taccaaagtc cttctaagtt tgctaaagaa
    15181 ccatcagcaa cgatgtggcc ccagccatct ttatagctca ttaattttgc aaattccatt
    15241 cctacttctt cttccatttg gcttgttgca ggagaagatt cataggcaac gttattacca
    15301 ttccatagca tagcaaaatt gtacgctagt aaagatggca tcaatgtttc agagttcata
    15361 tgaccccagt aacgtccggc attatgccaa ggaacggaat gggtacgcat ccgcgctgaa
    15421 atttctgata gaacatcctt tgttttattc acagtgtgtt caaaactagc actgcttttt
    15481 tcttctggcg taataatagg catgtcttga ggcatgtagt tttgacgcca gcctaaatgt
    15541 tcatccacca attcattcaa caaagcttta tagatttggc cgttctcagc tttgtcccca
    15601 atgaataggg catttaggtt tagatctttc gacaatgatt cactcatagt gaaaccctcc
    15661 taaaaaaata tttaaatttt gtcgagccct acattacacc tgttcttcta ttctgtaaac
    15721 taagttttgg cctgattttt aatcatattt taatatgtat caattatccg aattgttcac
    15781 acttttgaaa taattaaaaa aaacgaggga atttacaaat gtaaactccc tcgcaaagat
    15841 tgtattaacg acaaccagcg ttctatttta ggatgggttt caaaattgga atgttgtaat
    15901 agtatttccc tttttcaagg taaaaactta ctaaattcta atcaattact ttggccaaaa
    15961 agtaattttt cttacctttt cgaataacca caaacttacc atcgaattca tcggatggat
    16021 ttatgacaaa atttaaatcg gtaacgcggt caccgttaat acttatagct ccgtttgaaa
    16081 catcttcacg tgcttgacgt ttagatggtt caatttttgt ggaaacaagc agttccacga
    16141 tattttcagg tgtgcttgaa atttcaacgt tcggcatttt accgaatcct tgcgcgattt
    16201 cttccgcatt tagttcttta atatttccag agaacaatgc ttgcgttatt ttttgcgctt
    16261 ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg
    16321 cttcacgttt ctcaggttct gtctctactt ttttcgctaa gtcttcgatt tcttcttgag
    16381 acaagaaagt aaagaatttc aagtatttca ctacatcgcg atcatcttga ttcaaccaga
    16441 attggtagaa ctcaaatggt gaagttttct tcggatcaag ccaaacggca ccgcctgcag
    16501 tttttccgaa tttcgttccg tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt
    16561 ctgcttctgg tccttctaat tttcggatta ggtctaatcc agctgtgata tttccccatt
    16621 ggtctgctcc accgatttgc aattgcacat cgtatgtttt atgcagatgc aaaaagtcga
    16681 tagattgcag gatttggtaa gtaaactctg taaatgagat tcctacttcc aaacggctgg
    16741 caacgatatc ttttgctaac atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa
    16801 attcaagtaa agagattttt gacaaccagt cgtagttgtt cacaaatgtg atatttgcat
    16861 ctttaccaaa caattttctc atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt
    16921 ccatcgtttg caacacacgt tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc
    16981 cgccgccaat cagaatatat ggatgatggc ctgccaattg gaatcttttc atcatcataa
    17041 atggaatcaa atgtccgata tgcatcgaat caccagttgg atcaacgccg caataaagtg
    17101 aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg
    17161 cgtcgcgcca agttaattca tcaataatat tcattttact gacctccgtg tagttttttt
    17221 atgaaagcag gaacctgcta gcatgtgtaa ttaattattt cttcaggata ggataggaaa
    17281 taaaaaaagt cccttggcat ataagccaag ggacgaaatc tcgcggtacc acccaatttg
    17341 tatgtctaga tatccaacat acctctcgaa catttaacgc tgtcgcgtgg caatcactgc
    17401 caaagactcc tgaaatgtaa ttcgctttaa agcatgtact ggtttgcagc gaccaccagc
    17461 tttctgaaac attggcttta acaactactt cgtttcgatc ttcgtcttgt tattattaat
    17521 atgtgtctat catagagcgc aactttttct ttgtcaacag aaaaaggata ttttttttaa
    17581 attcctcatt ttttcttttt tgaatccgct ctaattccga acattttatt ttcaattatt
    17641 gttaaaagaa ataacttttt ctgtattttt tctgcaattt atcgagaagt cactttactt
    17701 tgcctcttat tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca
    17761 agggacgagg tatatggaaa aaaagcaaaa aat
//
LOCUS       DAXWLG010000047        17739 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXWLG010000047 DAXWLG010000000
VERSION     DAXWLG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.047"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            167..481
                     /locus_tag="ACGE4T_001242"
     CDS             167..481
                     /locus_tag="ACGE4T_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8386530.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            497..883
                     /locus_tag="ACGE4T_001243"
     CDS             497..883
                     /locus_tag="ACGE4T_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8386531.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            912..2297
                     /locus_tag="ACGE4T_001244"
     CDS             912..2297
                     /locus_tag="ACGE4T_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8386532.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2300..2453
                     /locus_tag="ACGE4T_001245"
                     /pseudo
     CDS             2300..2453
                     /locus_tag="ACGE4T_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            2476..3681
                     /gene="mobT"
                     /locus_tag="ACGE4T_001246"
     CDS             2476..3681
                     /gene="mobT"
                     /locus_tag="ACGE4T_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFF8386533.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            4465..6375
                     /gene="ltrA"
                     /locus_tag="ACGE4T_001247"
     CDS             4465..6375
                     /gene="ltrA"
                     /locus_tag="ACGE4T_001247"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8386534.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKRNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            6479..6703
                     /locus_tag="ACGE4T_001248"
     CDS             6479..6703
                     /locus_tag="ACGE4T_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386535.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            6820..7317
                     /locus_tag="ACGE4T_001249"
     CDS             6820..7317
                     /locus_tag="ACGE4T_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFF8386536.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            7404..7796
                     /locus_tag="ACGE4T_001250"
     CDS             7404..7796
                     /locus_tag="ACGE4T_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8386537.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            7780..10227
                     /locus_tag="ACGE4T_001251"
     CDS             7780..10227
                     /locus_tag="ACGE4T_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8386538.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            10230..12413
                     /locus_tag="ACGE4T_001252"
     CDS             10230..12413
                     /locus_tag="ACGE4T_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8386539.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            12410..13411
                     /locus_tag="ACGE4T_001253"
     CDS             12410..13411
                     /locus_tag="ACGE4T_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFF8386540.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            13426..14340
                     /locus_tag="ACGE4T_001254"
     CDS             13426..14340
                     /locus_tag="ACGE4T_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8386541.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            14654..14701
                     /locus_tag="ACGE4T_001255"
                     /pseudo
     CDS             14654..14701
                     /locus_tag="ACGE4T_001255"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14717..16636
                     /gene="tet(M)"
                     /locus_tag="ACGE4T_001256"
     CDS             14717..16636
                     /gene="tet(M)"
                     /locus_tag="ACGE4T_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8386542.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            16734..>17739
                     /gene="tet"
                     /locus_tag="ACGE4T_001257"
     CDS             16734..>17739
                     /gene="tet"
                     /locus_tag="ACGE4T_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HFF8386543.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     S"
BASE COUNT         5835 a         3141 c         3751 g         5012 t
ORIGIN      
        1 tttaataggt agacacttca aaaaatgagg tgtctatttt tttacccgat tttgaaagga
       61 agtgaactta tgaaaacaaa aaatcaagaa tcaaaaggtc gttccccact ctttaagacc
      121 atcaaacatt cattcagcca ataaaaaaga aaggataggt aaaaatatgg aacttaaatt
      181 tgtgattccc aacatggaaa aaacattcgg caatttagaa tttgctggcg aggataaagt
      241 cgttcagcga agaatcaacg gacggctaac tgtcttatca agaagctata atctctattc
      301 tgatgttcaa agagcagatg atattgtggt ggtgcttcct gctgaagctg gcgaaaaaca
      361 tttcggcttt gaggaacgtg tgaagttagt caatccacgt attaccgcag agggctacaa
      421 aatcggcact cgtggtttta caaattacct tttacatgct gacgacatga taaaagaata
      481 aagaaagaga ggaaaaatga tgagattagc aaatggcatt gtattagata aagacacgac
      541 ttttggagaa ttgaaattct ctgctctacg tcgtgaagtg agaatccaaa atgaagacgg
      601 gtcggtttca gatgaaatca aggaacgtac ctatgactta aaatccaaag gacaaggacg
      661 catgattcaa gtaagtattc ctgccagcgt gcctttgaaa gagtttgatt ataacgcacg
      721 ggtggaactt atcaatccca ttgcggacac cgttgctact gccacctatc aaggagcaga
      781 tgttgactgg tatatcaagg cagacgatat tgtgctgaca aaggattcta gttcattcaa
      841 agctcaacca caagcaaaga aagaaccgac acaagacaaa tagtcgctag gtagaaagga
      901 gactttttcg catgaaacag cgtggtaaaa ggattcgccc atctggtaaa gatttagtct
      961 ttcattttac gatagcgtca ctcctgcctg ttttcctgct ggttgtcgga ctgtttcatg
     1021 tgaagacaat ccagcagatc aactggcagg attttaacct atcacaagca gataagattg
     1081 acattcccta tttaattatc agtttcagtg tcgcaattct tatctgcttg ctggtagcgt
     1141 ttgtattcaa acgggttcgc tatgatacgg ttaaacaact ttaccaccgt caaaaactgg
     1201 caaagatgat acttgaaaac aagtggtatg aatctgaaca ggtcaaaaca gagggtttct
     1261 ttaaagatag tgctggtcgt acaaaggaaa agataaccta cttccctaaa atgtattatc
     1321 gacttaaaaa tggcttgata cagatacggg tggaaatcac gctgggaaaa tatcaagacc
     1381 aactcttaca cttggaaaag aaattagaga gtggcttgta ctgtgagctg acggataaag
     1441 agttaaagga ttcctatgtg gaatatactt tgctctatga caccatagcc agtcgtattt
     1501 ctattgatga agtagaagct aaagatggta aacttcgctt aatgaaaaac gtatggtggg
     1561 aatatgataa gctccctcat atgttgattg ctggtggtac aggtggcggt aaaacttact
     1621 ttatactgac actgattgaa gccttgcttc atacagattc aaaactgtat attcttgacc
     1681 cgaaaaatgc tgaccttgcg gacttaggtt ctgtgatggc aaatgtctac tatagaaaag
     1741 aagacttgct ttcttgcatt gaaacattct atgaagaaat gatgaaacgt agtgaggaaa
     1801 tgaagcagat gaagaactat aagactggca aaaattatgc ttacttaggt ctcccggcac
     1861 acttcttaat ctttgatgaa tacgtcgctt tcatggaaat gctgggaaca aaagaaaaca
     1921 ccgcagttat gaataagctg aaacagattg tcatgttagg tcgtcaagct ggcttctttc
     1981 taatactggc ttgtcaacgt ccagacgcaa aatatttagg cgacggaatc cgtgatcagt
     2041 ttaatttcag agtggcttta ggtcgtatgt ctgaaatggg ctatggcatg atgtttggca
     2101 gtgacgtaca aaaggatttc ttcttaaagc gaatcaaagg tcgtggctat gttgatgtag
     2161 gaacaagtgt catatcagag ttttatactc cccttgtacc aaaaggatat gatttcttgg
     2221 aggaaattaa aaagttatcc aacagcagac agtccacgca ggcgacgtgc gaagcggaag
     2281 tcgcaggtgt ggactgatct tgctggctgg tgtggcaata gccacgccag cacttaaccc
     2341 ctcggtatct aacagggggg tacaaatcga caggaaacag tcaaaaaaac attagaaaat
     2401 cctttggtta caagggattt acaaaatttc agcgtatgtc aaatgggctt taaaagttga
     2461 catacgcctt tttgattgga gggattttta ctgaatgaac aaacttggtt acagcattta
     2521 aaagaaaaac gcttggctta tggactatct caaaaccgtt tagctgttgc gactggtatt
     2581 acaaggcagt atctaagcga tattgaaaca ggaaaagtca agccatcaga ggatttacag
     2641 cagtcccttt gggaagctct ggaacgcttc aatcccgacg ctccccttga aatgctgttt
     2701 gattatgtaa gaattcgctt tccgacaaca gacgtacagc aggtggtcga aaacatctta
     2761 caactgaaac tgtcctattt tcttcatgag gactatggtt tctattctta ttcagagcat
     2821 tatgctttag gcgacatatt cgtcctttgc tcccatgaac tggacaaagg agttctggtg
     2881 gaattgaaag gtcgtgggtg cagacaattt gaaagctatc ttctggcaca acaaagaagc
     2941 tggtatgagt tctttatgga cgttttggtg gctggcggtg tgatgaaacg ccttgacctt
     3001 gccattaacg ataagacagg gattttaaat atccctgtac tcactgaaaa gtgccaacag
     3061 gaagaatgta tctccgtctt ccgcagtttt aaaagctatc gcagtggcga actggtacgc
     3121 aaagaggaaa aggaatgtat gggaaacacc ctctatatcg gttcattaca aagtgaagtt
     3181 tatttctgta tctatgaaaa ggactacgag cagtacaaga aaaatgatat tcccattgaa
     3241 gacgcagaag taaaaaaccg ttttgagatt cgattgaaaa atgagcgtgc ctattatgca
     3301 gtccgtgatt tactcgtcta tgacaatcca gagcataccg cctttaaaat tatcaatcgg
     3361 tatatccgtt ttgtagataa agacgattcc aaacctcgtt ctgattggaa actgaatgaa
     3421 gaatgggctt ggtttattgg gaacaatcgt gaacgattaa aactaaccac aaaaccagag
     3481 ccttactcct tccaaaggac gctgaactgg ctatctcatc aagttgcccc gaccttaaag
     3541 gttgcgatta aacttgatga aatcaaccag acgcaggttg taaaagacat tctcgaccat
     3601 gcgaaactga cagaccgaca caagcagatt ttgaagcaac agtcagtaaa agaacaggac
     3661 gtgataacaa caaaaaaata actcaaatac aaattcattg aatatagaga ggagaacatt
     3721 tttatgaatt ttggacaaaa cctttgtgcg actcgttcct tagtgaaaag ctaaggcact
     3781 aataaaagaa cgaaaaagtt tggttattag gagaactgat aatctgacag gtggaatgat
     3841 gaagtaacgc tctgaaacgc ctgccctgag cctccgacaa gcattgtatg aggaaactca
     3901 gaatgtttga agctcggtaa agacggctga aagataccgt aatagttgat gaacatatat
     3961 cttctatcga aaactatcca gaggtgggta gcgtatccaa caggtcgggg gtctataaaa
     4021 tatctatggt tagaatgttc tataaggaac tgacgaaaca gcgaatgtac gggtctaaat
     4081 ttgaactttg tagaaatgca aaggtacgag aaatcgtagc tggtgtggca aagtaagaat
     4141 ataggttatg aaatgccata taacgttaca ggcggtatca agccagcagg cttatagcta
     4201 gtgacctaag gatatatatg tatagataag attatcggaa cgaggaaagg tattgggttc
     4261 tcatcaagag aataaactga cgaagaacaa taagcagttg aactaatgct gaaaagcaga
     4321 gggcgaacct aagaaattct tgtaatgaga atggaggaat gccctcaagt cgtgtatata
     4381 ttagaaaagt attatgttgt ttcaataagg attacgagta agataaaaaa gctcactcaa
     4441 tttcaagtaa aggagtgaag ccttatgcct aaaaataaaa atgaaacatt gtgtgtagaa
     4501 gatttaagac acgctgaata ttacgaaatg caaaacacct ttgatgattt atatgccaaa
     4561 agtaagaatg gagatatttt cactcatctt atggatatta tcctatcgag agaaaatata
     4621 ttactggcat atcgaaatat caaagcaaat gcaggcagta aaacagcagg aacagacggt
     4681 acaataatta aagatattgg caaattgcca gcagaaacag ttgtcaaaaa agtaagatac
     4741 attgttgcag gcagtccaca tgggtatcga cctaagccag tgaggcgaaa agaaatacca
     4801 aaaccaaatg gtaaaaccag acctctaggt attccttgta tgtgggacag gcttatacaa
     4861 caatgtataa aacaagtatt agaaccaatc tgcgaagcta aatttagtga aaacagctat
     4921 ggttttagac ctaataggtc agttgaaaat gcaataaaag caacctataa ccgcctacaa
     4981 atcagccagc tacattatgt gattgaattt gatattaagg gtttctttga caacgtgaac
     5041 cattccaagc tgataaaaca gatatgggct atgggaatac gggacaagca cttgatattc
     5101 attttaaaaa ggatattaaa agctccaatc aaaatgacta acggtacaat aacatatcca
     5161 gagaaaggaa ctccgcaagg gggaataata tccccactac tagcaaacat tgttcttaat
     5221 gagttagacc attgggtaga aagccaatgg caggaaaatc ctgttacaaa aaactatgtg
     5281 gttcatatca ataaaagcgg tagcccatgt aaaagcaacg cttacaaaga aatgaagaaa
     5341 acaaagttaa aggaaatgta tatggttcgt tatgcagatg attttagagt tttctgccgc
     5401 tacaaggaaa gtgctgaaaa agccaaaata gcaatcactc aatggattga acaacgatta
     5461 aaattggaag tatcacaaga aaaaacaaga attgtaaatg ttagaaaaag atactccgac
     5521 tttcttggtt tcaagattaa aatgataccg agaagaaaga agttagttgt taaatcacat
     5581 ataagtgata aacaattcaa aaaccaaaaa gacaagctgg tatcacaggc aaagaaaatt
     5641 gcttctccct caaaagggaa aacggaatta gaagaaacaa gactttataa ttcaatggta
     5701 ttaggtatgc aaaattatta tcagattgca acgaatgtaa atctggattg tatgaaactg
     5761 aatagagctg taatggctat cttcactaac agattaggta caaacaaaag gggcagattg
     5821 agaaaaacag gcagacaact tacgacaaag gaggcaacaa gatatggtaa atctaaaatg
     5881 cttcggtatg ttgctggcga gggcgaacct attttccctg ttggatatat ccaacacaga
     5941 aaaccaatgg caaaggtgta tttagcgaat tgctacactc tagagggaag aaaatttatc
     6001 cacaccaatc taagtgttga taaattactg atgtaccagt taatgaaatt atctttggaa
     6061 aatcgcacaa tagaatacgc agacaatcga atttcgctat tttctgctca acatggaaaa
     6121 tgtgctataa ctctggaaga atttcaacaa gctaatgaaa tacattgtca tcatattgta
     6181 ccaactggag ctggtggaaa tgacgattat aaaaatctta ttttggtaaa agaggcggta
     6241 caccgtctaa tacacgctaa aacagaagaa acaatacaaa aatatatagc tctattgaag
     6301 cgaaataaag tccagctcat aagactaaat aaatatagag tattagctgg caatcaagaa
     6361 ttaaacttga tataggtcta ctacataaat acttttcaat ataaacacga tggaacgcgg
     6421 agtgcggtga aagtcgcacg ctccgtgtga atggggggaa aagtttgaga tatttataat
     6481 atcaggaact tacctatcca tatataactg gtttctatca aacgctcaat cactggtgct
     6541 tttagcaatc gttgtgattg gcttgtatct tggcttcaag cgtgagttta gcaaactgat
     6601 tggcttttta attattgcga ttattgcggt tggcttagtc ttcaacgctg ctggagtaaa
     6661 agacatttta ctagagctat tcaatcgcat tattggtgct taaataaaac cgttcttttg
     6721 tggaatataa gtggttttct tatgttccgc aaaggaatgg tacaccaaac gaagtgcggt
     6781 agggattttt gaatctctac aaagaaagga cgtgaatata tggacgatat gcaagtctat
     6841 attgcgaatt taggcaaata caatgagggc gaattggtcg gtgcgtggtt tacctttccc
     6901 attgactttg aggaagtcaa agagaaaatc ggcttgaatg atgaatatga ggaatacgcc
     6961 attcatgatt acgagttacc ctttacggtt gacgaataca cttctattag cgaactcaat
     7021 cgcctatggg aaatggtatc ggagttgcca gaagaactac aatcggagct atctgctctg
     7081 ctcactcatt tttcaagtat tgaagaacta agcgaacatc aagaagatat tatcattcat
     7141 tccgattgtg atgatatgtc tgacgtggca cgctactaca ttgaagaaac gggtgcttta
     7201 ggcgaagtgc cagccagtct tcaaaactat attgattatg aatcctatgg tcgggattta
     7261 gacctttcgg gaacgtttat ctcaaccaat cacgggattt ttgaaatcgt ctattaaatc
     7321 tgtcggtaca ttcttactgg cagattttct tgtttacggg gtagtcaaaa gactatcccc
     7381 atttttatga aaggattgat tacatgaaga aaatacgaag ctataccagt atctggtctg
     7441 ttgagaaagt gttgtactcc atcaatgact ttagacttcc gtttcccata acctttacgc
     7501 aaatgacatg gtttgtcgtg tcactctttg cagttatgat acttggcaac ttgccccctc
     7561 tttctatgat agagggagca tttctcaaat actttgggat tcctgtggct ttcacatggt
     7621 ttatgtctac aaaaaccttt gatggtaaaa agccttatgg atttttgaag tctgtcattg
     7681 cttatgcact gcgacgaaag ctgacctatg caggaaaaaa agtaacgctt ggcagaaacc
     7741 agccacaaga agccattaca gcagttagga gtgaatttta tggcatatcc aattaaatat
     7801 attgaaaaca atctggtctg gaataaagat ggggaatgtt acgcttacta tgagcttgtt
     7861 ccctacaatt actcatttct aagtccggaa cagaaaatac aagtacatga ttctttcaga
     7921 cagcttatcg cacaaaatcg tgatggcaag attcatgctt tacaaatcag tacagaatcc
     7981 agcatacttt ctgcacaaga acgttccaaa aatgaagtca ctggaaagct caaaacggtt
     8041 gcctatgaca aaatcgacca acagacagac gctttaatat ccatgattgg cgaaaatcaa
     8101 gtggactacc gtttctttat cggttttaag ttgcttctca acgatcagga gttttctatg
     8161 aaaagtctta ccgttgaagc aaaaaatgct tttactgatt ttgtctatga tgtgaaccat
     8221 aagctgatgg gcgattttgt tagtatgagt aatgatgaaa tcctgcgttt tcagaagatg
     8281 gaaaagcttt tagaaaataa aatctcccgt cgtttcaaaa tccgaagatt agataaggat
     8341 gacttcggct atctgattga acacctttac ggacagacag gcactgccta tgaagagtat
     8401 gagtatcctc tatcaaagaa aaagctggaa catgaaacac tgattaaaac ctatgacctc
     8461 attaagccta ctcgctgtct ggtggaagaa aaacagcgat atttgaaaat ccagcaggaa
     8521 gacgaaaccg cctatgtagc ttactttacc atcaacagta ttgtcggcga actggacttc
     8581 ccgtcctctg aaatcttcta ctaccagcaa cagcaattta cctttccgat tgatacgtca
     8641 atgaatgtgg aaattgtagc gaatcgtaaa gccctatcta ctgtccgcaa taaaaagaaa
     8701 gaactgaaag acttggataa ccacgcttgg caaagtgata atgaaaccag ctctaatgtg
     8761 gcggaagctc tggaaagtgt gaacgagctg gaaaccaatt tagaccaaag caaggaatct
     8821 atgtataagc tgtcctatgt tgtaagggta tcagcaaatg accttgacga acttaaacgt
     8881 cgttgtaatg aagtgaaaga tttctatgat gatttgagcg tgaaactggt acgacctttt
     8941 ggggatatgc tgggcttaca tgaagaattt ttacctgcca gcaaaagata tatgaatgac
     9001 tatattcaat acgtgacctc tgatttcctc gctggtttag gttttggtgc tactcaaatg
     9061 ctgggtgaaa atgaggggat ttatgttggc tacagcttag atactggacg caatgtctat
     9121 ctgaaacctg ctcttgccag tcaaggggtt aagggttcag taaccaatgc gttagcgtct
     9181 gcctttgtcg gttcgctggg tggtggtaaa tcctttgcga ataaccttat cgtctattat
     9241 gcagtgcttt atggggcaca agcagtgatt gtagacccaa aagcagaacg tggcagatgg
     9301 aaagaaacct tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag
     9361 aaaaacaaag gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca
     9421 ctggctattg atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc
     9481 ccaatcctta gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa
     9541 gtgattgaag aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc
     9601 gaaagtttta cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag
     9661 tctatcagcc ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac
     9721 aaggaaactt cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt
     9781 gtcattagta cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta
     9841 gacttagacg aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag
     9901 ctggttcgtg ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat
     9961 gacctcttag atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact
    10021 gaccttaacg agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac
    10081 aatcagaagc gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt
    10141 cgtgtcggtg tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc
    10201 agaccacctg tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat
    10261 caaactggac gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga
    10321 ttgccatttt tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg
    10381 atacggtcaa tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt
    10441 tttatgtgga taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac
    10501 aagtcatgta tggactatat gccattacca attttatttg gacaatcagt ctttatgttt
    10561 ccaatgcgac gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag
    10621 cagattccat tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa
    10681 cagagggttt ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg
    10741 cctatacagg actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg
    10801 tgctggtttt tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa
    10861 tcaatgactt ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca
    10921 tgccccattc agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt
    10981 ccatacaggt tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta
    11041 ttggtattga ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag
    11101 acagagaaaa aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta
    11161 caaagacgat taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt
    11221 ccatatttgt attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct
    11281 atgctatgtt tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt
    11341 caaaacgagc cataacaaag ctctttaata ccattttgac acgagctgga atcacattga
    11401 ttattacgac agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt
    11461 tctttttgat tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg
    11521 atttaatgag tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga
    11581 tgagaaaacc tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac
    11641 gctccgtgac tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac
    11701 agtcgggttc gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg
    11761 aaaaatcaac actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag
    11821 acagaatggt agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg
    11881 caagatatgc agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca
    11941 gaagtatttc tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa
    12001 gacggaaaac cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc
    12061 cagcttcttc cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga
    12121 cttcaaggca aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc
    12181 gtccaacagt taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc
    12241 aagaaagaac ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac
    12301 gttcaatcac aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa
    12361 gtagaccacc aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa
    12421 aactttagtg attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt
    12481 cgccatttta ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt
    12541 ggatgtttcc gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata
    12601 tggaattgaa gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac
    12661 agcagaagat gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac
    12721 agaagaatcc attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag
    12781 gcttggtgta gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg
    12841 atatgtggct aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga
    12901 gtattcgggt gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg
    12961 ctggcgatac aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac
    13021 aacagagttt gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg
    13081 ctggaagtat gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac
    13141 acaatggacg tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga
    13201 tgtaacccgg catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc
    13261 tacctataac gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat
    13321 gtttcatgca ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca
    13381 tttagtggga gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga
    13441 aaaaatcaga atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc
    13501 aatcctcgta aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt
    13561 gcgatattca agcactttac agccattgat acccatacca ttcatgaaac caccatcata
    13621 gaaaaggaat atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc
    13681 tactattcat gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca
    13741 tatctgacag aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta
    13801 tcgtcttctg taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt
    13861 gatgtaacct acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac
    13921 tctgcttata tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat
    13981 ccgaccatta ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag
    14041 ggtacggttg attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag
    14101 ctctatccta cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa
    14161 ccaatcggaa aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat
    14221 aatcaagtca cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag
    14281 gtatctcaat ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa
    14341 caaatattgg tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt
    14401 ggagattcct ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag
    14461 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat
    14521 aattggatgt ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta
    14581 tcatgtgatt ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca
    14641 taaaaatccc agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg
    14701 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg
    14761 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
    14821 cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac
    14881 aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac
    14941 gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc
    15001 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc
    15061 acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
    15121 tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa
    15181 acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg
    15241 ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt
    15301 agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc
    15361 tgtttatcat ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac
    15421 gaataaattt tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa
    15481 aattgaatat tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct
    15541 gcatttgcgg gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta
    15601 tacttcaata aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat
    15661 tttgcaaaat gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca
    15721 gagagagaga attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc
    15781 tcaacaaagg gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct
    15841 acaatattat gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca
    15901 aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga
    15961 gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga
    16021 agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg
    16081 aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa
    16141 tgcagttatg gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac
    16201 ggattgtaaa atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga
    16261 ttttcggatg cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt
    16321 gttagagcca tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata
    16381 caacgatgct cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt
    16441 cattcttagt ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt
    16501 ctttacaaat ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg
    16561 tgaacctgtt tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt
    16621 caataaaata acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag
    16681 atgaaatatt ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt
    16741 gtaatgaatg taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt
    16801 tttttagcgt attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt
    16861 ttaataaacc acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca
    16921 ttggaacagc tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat
    16981 ttggaattat aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt
    17041 ccttacttat tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg
    17101 taatggttgt agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta
    17161 ttggatcgat agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc
    17221 attatattca ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt
    17281 ttcttatgaa attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa
    17341 ttatactaat gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt
    17401 cttttcttat cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aagaaagtaa
    17461 cagatccttt tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt
    17521 gtgggggaat tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga
    17581 aagatgttca ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa
    17641 tgagtgtcat tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat
    17701 acgtgttaaa catcggagtt acatttcttt ctgttagct
//
LOCUS       DAXWLG010000048        17394 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXWLG010000048 DAXWLG010000000
VERSION     DAXWLG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.048"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            167..1195
                     /locus_tag="ACGE4T_001258"
     CDS             167..1195
                     /locus_tag="ACGE4T_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386544.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1221..1661)
                     /locus_tag="ACGE4T_001259"
     CDS             complement(1221..1661)
                     /locus_tag="ACGE4T_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8386545.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1821..2327
                     /locus_tag="ACGE4T_001260"
     CDS             1821..2327
                     /locus_tag="ACGE4T_001260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8386546.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2495..4408
                     /locus_tag="ACGE4T_001261"
     CDS             2495..4408
                     /locus_tag="ACGE4T_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8386547.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4413..5414
                     /locus_tag="ACGE4T_001262"
     CDS             4413..5414
                     /locus_tag="ACGE4T_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8386548.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5616..5822)
                     /locus_tag="ACGE4T_001263"
     CDS             complement(5616..5822)
                     /locus_tag="ACGE4T_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFF8386549.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(6468..7316)
                     /locus_tag="ACGE4T_001264"
     CDS             complement(6468..7316)
                     /locus_tag="ACGE4T_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8386550.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            7480..8382
                     /gene="gnd"
                     /locus_tag="ACGE4T_001265"
     CDS             7480..8382
                     /gene="gnd"
                     /locus_tag="ACGE4T_001265"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFF8386551.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            8384..9385
                     /locus_tag="ACGE4T_001266"
     CDS             8384..9385
                     /locus_tag="ACGE4T_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFF8386552.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            9382..9666
                     /locus_tag="ACGE4T_001267"
     CDS             9382..9666
                     /locus_tag="ACGE4T_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFF8386553.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            9699..10496
                     /locus_tag="ACGE4T_001268"
     CDS             9699..10496
                     /locus_tag="ACGE4T_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8386554.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            10510..11343
                     /locus_tag="ACGE4T_001269"
     CDS             10510..11343
                     /locus_tag="ACGE4T_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8386555.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            11361..12218
                     /locus_tag="ACGE4T_001270"
     CDS             11361..12218
                     /locus_tag="ACGE4T_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8386556.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            12240..12749
                     /locus_tag="ACGE4T_001271"
     CDS             12240..12749
                     /locus_tag="ACGE4T_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8386557.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            12786..13187
                     /locus_tag="ACGE4T_001272"
     CDS             12786..13187
                     /locus_tag="ACGE4T_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386558.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            13299..13652
                     /locus_tag="ACGE4T_001273"
     CDS             13299..13652
                     /locus_tag="ACGE4T_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386559.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            13686..14129
                     /locus_tag="ACGE4T_001274"
     CDS             13686..14129
                     /locus_tag="ACGE4T_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8386560.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            14146..15231
                     /gene="uxuA"
                     /locus_tag="ACGE4T_001275"
     CDS             14146..15231
                     /gene="uxuA"
                     /locus_tag="ACGE4T_001275"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8386561.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            15224..15937
                     /locus_tag="ACGE4T_001276"
     CDS             15224..15937
                     /locus_tag="ACGE4T_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8386562.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(16144..16293)
                     /locus_tag="ACGE4T_001277"
     CDS             complement(16144..16293)
                     /locus_tag="ACGE4T_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386563.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            16689..16889
                     /locus_tag="ACGE4T_001278"
     CDS             16689..16889
                     /locus_tag="ACGE4T_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8386564.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         6069 a         2694 c         3628 g         5003 t
ORIGIN      
        1 aatccttaga aaatcttagt caaaaaatta tataagtttg cgtacgattc aaagaaaatt
       61 aagagaactt aatactattt tgaaaaacta tcatttatct tgcagcttga aaaaaggtaa
      121 tccgatagaa ggagaagaat accgtatccg atactttttt cacttgatgt attggcagtc
      181 attcgacgaa aatcattctg atatattatc aagtataaaa aataaacgtc aactattaga
      241 aaaatttgcg aaacatgcgc catatacgcg tagtatagat agaaagaaat ggatcagcct
      301 actaagaatt tcgttgcaac gaatgaaaca aggccactcg attacgtatg tcccagaaga
      361 gatgaacaaa tttgtgcatc cattgataac aaaagaagag tttacgcatt acatactaga
      421 gccttttttt gaagcaaact tctggtcgat gaatcacatc actccaacag agcaagctta
      481 cctttatttt atgtttggtg taatgaacac ctatttggaa gaagatttgt tggatactcc
      541 agataaagta caagaaagtt ggggctggat tgaatcgtct attgtagaaa attttgaaaa
      601 aaacttcaag atttgtttta caacttcaga aaaaaattat ctatatgtca atttggcaat
      661 gatccatttg tattcaaggg tttttgggac aaaaaagaag gtggatgctt ttggaaaatc
      721 agcagaagac agagattttc agcatatatt tccagtgatg tatccaatca tgcaacagtt
      781 ttttagtggg gtagctcgca gaattccaga gcttaatact tattatgaaa agaacagacg
      841 gttgattttt caatactgta tgttagtcag ggatattttt ataaaacacg aacaacctgt
      901 catattctat attcaatcaa agtatggtaa aacacaggaa ttatgggcaa agaaaaggat
      961 attatctatg actgaacgcc caataaaata tagtgcatcc gctgatcagt tacctgatat
     1021 tgtcattagc gattatccta tcgacaaaaa ggcatatgat caaacgaata cacagatatt
     1081 ttactggaat ggacaaccta caaaaaatga ttgggcgcgt ctgaaagctg aaatcataaa
     1141 aatcaggaat gagaaaaata gatccatatt ggaaaaattg gatttcgtta attaagaaaa
     1201 gcagattgtt tatgccaaaa ttaagacatt gcaaaaagat tattttcttt aaaatcgaca
     1261 agatgaccag aaagcaagag ttcgttgagc tcttgctttc ttttagtttt agaaaggaac
     1321 atcctgattt tatcttcgct aaatccagtc aaaagttttg cagattggtc gtagataatc
     1381 ggttttttca ataaagaagg ggatgtacga atctcaatat atagttcact caaactgagt
     1441 tcatctattc tgtgaacaag atttttataa ctaatagaat gagtagagag catttcggtc
     1501 acgccgttgt ttgtcaattt tagtaagtgg ataaattcat ttttggttag tggttgacga
     1561 attaagttct gttcgataaa cggtatatca tggacgataa gccaacgttt tgcgcgtctt
     1621 gaagacgagc agccagaacc tgtatacaat ttaatcatca tacattcctc caatagctaa
     1681 gtatttatga aataataaca tgaagaaata gtcgaaaagt gccatttaga ggtgtaaaat
     1741 aagaaaatgt tgtcacgtat aatggaacta gctagataag aaatactatt aaatcaagaa
     1801 aaaagaaaga aggcacaagt atgtcaaaca cagccgacgc aatagtagtg atcgatttac
     1861 aaaaaggggt atgttttgat tcttcgccga tacacgaatt agaaacagtg acagcattag
     1921 tgaataaaag aatcaaaaca tacgaaacga ttgggaaacc agttattttc gttcaacacg
     1981 aagatgctca attagtgcca caaacaaaac catgggagct attgtcggaa ttatctattc
     2041 ctgagcacag ttattttgtg agaaaaaccc atgcgaattc attttataag actaatttga
     2101 gtgttttatt aactgaattg gactgtcatt cgatcgaatt atgtggggca caagcagagt
     2161 attgtataga tacgactgtg aagtttgctc atggattagg ataccagctt atgatgtgta
     2221 gaggagcaac gacaacttat gataatcctt ttatgaaagc aaaggaaaca atcgcttttt
     2281 acgagaaaat ttgggataaa cgatttctga catttttgga gcaataagtt gtttcttcgt
     2341 ttaaaaaaag cagaatggat actggtttat ccattctgct ttattacgta aaatgagaaa
     2401 ataatcctgt tctattttta tgggataaac catttatttt ttgtataaaa aatgtttgcg
     2461 tttacatata attctcctag aaacataaaa aggagtgaat agcttgagaa caaaacaagt
     2521 attaaaggga gcgttggtca tcttatttgc tgtaagtaca agcgtcccgc aatggacagt
     2581 atatggagag agcatttctt ccgaatcgga atctcgggag gtagaaacca cagaaactac
     2641 tgatgctgga aatgaaacga aatcgagtac ttcacaaaca gaaacaaata ctgaaacaac
     2701 ggttgagaca tctgttacta gcacagatca agaaacgacc gctaccacag ataaccaaca
     2761 aacagaagaa gcaacagcta caaaaatgtc aaacgacgaa tcctcttctg ctaaagtaag
     2821 tgacaaagag aatgggacag aaaatgatga tatcggtaaa ggtgaaaatt tcaatggtaa
     2881 agaatatttg aattacttcg gtaaacagat gacagaaata cttaatggac caacagaaga
     2941 catcaagtca aaattaatag agttattagt cactgagctt gctgttaatg attatggtgt
     3001 agataaagag attatcaaac atctgattgc tacttatcta ttgtacgata tcgcttttgc
     3061 aaaaaacagt gtgttgatgt cgatgcttgg aggtacagat gatgggacaa cgcctacaga
     3121 aaacacaggc ttctatccaa atacaaaaca acttcatcaa actgtactaa acaaacaaga
     3181 tggcaaaatg atggatgtat atgcatatta tgtcgatcaa ggttcagata aaacagtcat
     3241 gatccatggc ggatttcgag ggaattggaa taacgggatc gttacagaag aatacaatga
     3301 tttttataag gccgggtata atttattatt tgttgattcc agagcgactg gtaatagcgg
     3361 aggcgattat gtgacctatg gacaatatga gtcagatgac gtcctttact ggataaatca
     3421 agaagttcga gagcggccat ctcaaaagat tttactttat ggtggttcga tgggagcagc
     3481 tacgatgatg tctgtattag cgaaagatat tcctgtcaat gtcaaaggaa ttattgaaaa
     3541 ttgcggattt gcatcaattg atgaacaact acgttttacg tacagccaga cagtagcccc
     3601 cgcactaccg gatgccataa aaaatcagtt agacatcatt ggggatcaag agcatgaaga
     3661 tttatttatg ggccttttga aacaatacta ttttgatcag gagatgcatt tggatacaaa
     3721 agctgctcta cctacgattg gaatgtcaga ttcattgcct aaattgatta tccatggaac
     3781 agcagatgat gtagtacctg tgtctaacgc gcaaaaatta tatgagttat caggagggta
     3841 taaagatctt ttattagtag aaggagcagg gcatggaaag gcacaggaag ttgaccatgc
     3901 tgcctacacg aaacatgtaa cggacttttt gaaagtagtg ttccatgatc agataaacgt
     3961 aaagtatgtg gatgaaaaca ataaaagtct actcaaagac caggacgaaa tccagcttta
     4021 cggagcttat ggagaaaatt atatgacaga gcaaaagaca ttcgaaggat acgaattagc
     4081 aaatgttgaa ggaccgactg aagggatttt taatgagacg ataccaacta ttattttcta
     4141 ttacaaaaaa atcccagtag ctccaccaaa aaagcaagat ccgacagaga atgcagacaa
     4201 taagggaaag aatgaacctc gagaggatca atcaagtgat gatacgcaac cgattcagat
     4261 gacagtgaaa gtacaaaaaa agacagatgc aaaaaaaaga atgttgccga aaactggaga
     4321 aaagaaacat tcgcttctca tttattcagg tggattttta tctgttagca cgattatcac
     4381 atggatttgg cgaagaaaga gagggtaaat gaatgaaatg gttcaagcga atagtgattg
     4441 cagtaccagt tatcatcgta gttattttag tcactgtatt tttgattaat caaatcacac
     4501 caaagccgat tagcttgctt gttagaaaac aatttgacac aagtgaaaaa gaacaagtat
     4561 atgcccgacc agatgatttc cagcaaaaaa tcgaacaaat cgatataaaa aaagaaatat
     4621 cctatccttc aaagtacaaa aataataata tggatatcta cacaccgaaa gtggaaaaga
     4681 aaaaattacc gattcttttc tggatgcatg gaggcgcata tgtaggaggg gataaaaatg
     4741 attgtcgtga ttatttggaa tatctatgtt cagacacgca gcaaatcatc gtaaatatag
     4801 attatgaacg atcaccggaa gcaaaacatc ctacaccagt tatccaattg aacgaagcca
     4861 ttcaagcagc taaaaaagag ataaaagatc aagcagattg gtcgaatatt tcgattggtg
     4921 gaaattcggc aggtgcacaa attagtggag aatatttgtt agcacttcaa aatgaagaaa
     4981 ttaaaaaagc tgatcatctt gatccgactt tggagaacaa gcagattacg aagtttattt
     5041 ctttgtctgg tctgttggat ccttctgcac ttactcaagt aagtgataaa atctctagct
     5101 ttctttatga aaaatgtgga tgggcttact ttgataataa ggactttgaa caatcagaga
     5161 aggtcaaaag tttagccttg gtcagacatg cggatcgttg gacgcaaaac acattcttaa
     5221 cggatggaaa tacaaatacg tttacgaaac aaatggaaga agtaacttct gtatttgaga
     5281 aagcgggggt gaaagtcaca aaagtagatt atgcggagaa aaatgctgtt ttgaaccatg
     5341 aatatcagtt tgatttcagc aataaacaag cgcaagaaac atatcaaaag ttagtaatgt
     5401 ttttcaagga gtaaagtctt atgatgcaaa agaggttgta atctgtcact tagctccaag
     5461 tcacaaggag caatttcaat aaacaagctt ttcatggttt atgaatattg taacttgtga
     5521 ctgaggagtt agagatggca aggggcacct tttattcgaa atttgggaat atcacacgat
     5581 tattgtcacg atctctttta cgtaaatttt ataagtcaga tttgttgttt tgatcgttta
     5641 aaaaaagcaa caaatccgaa gcctgcggag gcaagaaggg acaaaaagcc gagtggtaag
     5701 caaaagaatg ctggttccac taatattccg ttgttattga tataaggctg ggaacaaaga
     5761 tgaatagcaa ataaaatcaa tgcactagcg aaaaaaagca aagaaatatg gatagctttc
     5821 ataagcagca cctccttttt tataattgta tgataaatag attaagaatc tataaatgtg
     5881 acaagtttgt taggatgtga ataaaatgat catctagcga acttgaaaca aagatggcta
     5941 atgataagca attaatgatt cagcaatcag ataggtacaa agtttaccat catacgtcta
     6001 gaccaataat agaaaatatg ggaatataat agaaaagatt taggataccc ccaaaagaat
     6061 ttccccaaaa attcgatgta tcctaaactt ttttatttta cataacaatc atatcaactg
     6121 tgaaataaaa ttacaaatga taacctaaaa tgaggccaaa agccagtgtt atcaaggaaa
     6181 tacttaattc accaaaattt tttttaattt tttagaaaat aagaaacaaa aataataaaa
     6241 aaaagaaaag ataaattttt tactactgta tactaacttt gttatcgcat ctaacaagta
     6301 actcgaataa taccaataca accttctcca aagatgcgat gacatacgtt ctaacccacc
     6361 gactattgat gttctgtacc acccttgcag aaccaaaaaa gccactacca aatatttggt
     6421 agtggctttt tttgttttat ggatttttcg ctttttctgt tatggtgcta ttctttgatt
     6481 tcagaaatag tatgtcgaac tttagcgata gaacgacgtg attctttttc gttgtaaaac
     6541 atcaaaatca catatagaga gtccaaaatg cttagttgag aaatacggga agctaaagct
     6601 tcagatcgga attctgtttc ttcagaaata gaaataaaaa cgacatcgcc aagtttagca
     6661 agggaagaat tagcttggct agtaataacg attgtttttg ctccagtttt tcttacggtt
     6721 tcagcaatat ggattgtttc ttttgattgc cctgtgtgag aaatcaaaac agcacagtct
     6781 ttttccgtca ataaagaagc ttccatcaat tgaatatggt aatccgtgct gtgagaagtt
     6841 gcgatagggg aacgcaaaaa tttatgataa gcgtcagttg ctacaatctc tgaaccgcca
     6901 acaccgaaaa aatagacacg tttagattca ttgataatag tagccgcttt ttttagatcc
     6961 ttttgttgta acaacttttt tgtgtcattt aaagttgaaa tactagactc gaacaccttt
     7021 tgcgccatag tgagctcagt atctgttttc tgaatatttt cgtggataga aatagcagat
     7081 agatcatttt cttggatcaa catggccatt ttaaaatctt taaatccatt ataaccaagt
     7141 ttttttgtaa attgaaaaaa agtagagtca gcaatgccca gttcacttgc taaattgcta
     7201 ataggactat gacttacttt gcttggattt tctaaaatat aatcggcgat agcctgttct
     7261 tttgggctga agtcgttgta cattgttttg atttgtagtc ttatggagac gctcatgaga
     7321 attcctcatt ttcataataa gattatcatt ttttccgttc tcattaatgt taaagataca
     7381 aggagagtag gaaaaaatca aacaaaaaat atttttgtaa aatctattta caaaaaaatt
     7441 tttttgtata atgagggtat aagaaatgga ggcgctatca tggaaattgg tgtaattggt
     7501 ttgggaaaaa tgggattgaa tttagcttta aacttaaaag agcaccatca caaagtacaa
     7561 ggactcgaca tttcaagtac agctgttagt caggcaagag aaaatgacat tcctgcttat
     7621 caagagatct ctgaatttct agctactttt gagcagaaaa aaatcatctg gttaatgctt
     7681 cctgctggag agataaccga aaacatgtta agaaaacttt ttccaaaact cggaccagga
     7741 gacattatta ttgaaggcgg aaactcttat tataaagatt caatccgtcg agcagaagag
     7801 tttcagaaaa atgaaatcgg ttacttggat tgtgggactt ctggaggaat cgaaggagca
     7861 cgacatcaag cctgcttaat gattggagga gataaagaaa cttttctatc cgtggaacaa
     7921 ttattcaaag atgtagcaat tgaaagagga tatctgtatg ctggtgcatc cggaagcgga
     7981 cacttcttga aaatgatcca taatggaatc gaatatggaa tgatgcaagc aatcggtgaa
     8041 ggattccaac ttgtacaaaa aagtgagtat gcttttgact tggaacaagt cgcacgtgtt
     8101 tggaatcatg gttcggtgat tcgcggctgg ctgatggaaa tcatggaaga tcagttaaac
     8161 gaacatccca atttaagtaa ttatcgggga atcgtttctg catcaggtga agcaaaatgg
     8221 acggttgaaa cagcgctaga aatggctgtt ccagttccag taattgcaac tagtttgttc
     8281 atgaggaatc tttcacagga agaagatagt ttttcagcaa aagtagtttc agcactaaga
     8341 aatgggtttg gcggacatga aatagttgaa aagggtgagt aaaatgccag attatcatat
     8401 agcaatcgtt aattccagca gtttcggaag agtctttcca caccacttgg aaagattaaa
     8461 gtcagttgga gacgttcatt tctttactgt tgatcaagag attccaggaa aagaactagc
     8521 ggagttactt aaaggattca atatcattat tgcaagtgtt acgccatttt ttcgacgaga
     8581 tttttttgaa aacaaagatg aactgctatt gatttcccga catggtattg gttacaacaa
     8641 tattgattta aaagcagcaa aagaacacca gacaatcgta tctatcatac ctgctttaat
     8701 tgaacgaaat gctgtagcag aaaataatat aactaatttg ttgtccatgc ttcgacgcac
     8761 gacacaagca caagcaagag tcaaggacga tcgatgggaa gatcgggcgg agttcgtcgg
     8821 taggacgctg tttaacaaaa cagttggtgt gattggtgca ggaaataccg gaagcggagt
     8881 aatcgaaatt ttgcgtaacg gttttcgctg tgatgttcta gcttatgatc cttacaaatc
     8941 agatttatac atcgaaagct ttggtgcaaa aaaagtagaa cttcaagaat tactggaaca
     9001 atcagatatc atttgtttat gtgcgaattt gacagaagag aactatcaca tgatttctga
     9061 taaagaagtc gatcagatga aagaaaatgt ttatctatct aatagtgcaa gaggcgctct
     9121 agttgatgaa gaagcgatga ttaatggtct gaaaagcgga aagatcgcag gatatgccac
     9181 agatgtttta gaagaagaac cgggacgtca agatcatccg tatttagcgt ttgaacaagt
     9241 agtgattacg ccacatatat cggcctacac aagggaatgt ttagaagcaa tgggtgaaaa
     9301 atgtgtcaga gatgtcgaac agatcagtca gcaccttctt cctgaacgtt ctgtccagac
     9361 aaagagcaac tatataaaag gatgatgaag aaaatggtga acggtttaaa agtgaaaaca
     9421 ggccctcaat tttatttata tgaagaaggt ggtatttcaa aagtttctga tctcttgaaa
     9481 tcatacggcg cgaaaagagt actggtcacc cacggaacgg tatcatggga aaaggcgcta
     9541 cctaaattag tatttttgaa tgacgaaacg attcaatttt tttatcatcg ttattctgga
     9601 gaatgtagtt atgctgaagc cagacgaata gctacaatca tcaaaaaaat gaaatcgatt
     9661 tcgtagttgg cgttggcgga ggaaaactga ccgatctagt gggttatagt gccaatcttg
     9721 caagaatcga tttcggagtc atccctactt tagctagtaa ttgtgctcca tggacacctc
     9781 ttgctgtcat gtataaggag aatggacaag cagaagggaa aacagaacac ttttacagac
     9841 aagctgcttt ttttataaca gatccccaat tagtgattga ttcccctgtt ccatatttca
     9901 ttgccgggtt agcagataca ctagcaaaat ggtatgaatc tgagctcatc ttagaacaag
     9961 attttttgca agatgaatca tttttgaagc tagcacaaga tactgcgaag atctgcaaag
    10021 aagagatttt gaatcattct gctaaagcaa tccaagatat ggcacaaaga aaactgacat
    10081 cggagttcaa acagctttca gagattatct ttgctgttgc tggtttagtt ggaggattag
    10141 gagataaata cgcaagaaat gcggcagcac atgctatgca tgacgctatc agtaaatata
    10201 ttccggaaag ccatcgtttt ctacatggag aaaaagtagc gtatgggatt ttctatcaac
    10261 tggcactaga aggtcgctgg gaaaaaatag acgagttata tcctttgtat cgggaattag
    10321 aactgccggt ttctctagaa caaatgggat tatttccgaa agataaacag ataatagata
    10381 aaatggctga atttattgat tcgaaagaga aagtgcattt gattcctata gaggtaacac
    10441 agaaatcact gatacagtca atgatggaac tggaaggtta tatcagaaag atctgaagga
    10501 ggaggacaca tggaaacatt aagtgtagta caaagtttgt tgattgcttt atgggtagcc
    10561 gcggttatgt cccgctggtt aggtggagga gccacgttga ccttacgttt ttctcctttg
    10621 atgactggat tagtcgtcgg gttgatcatg ggggaagttg cacaagcgat gattgttaca
    10681 gccgctttgc aaatgattta tatgggggtt ttttctccag gaggatcaat gccagctgaa
    10741 ccatctattg cagcagctat cgcagtacca gtcgcattat taggggatct gtcgccagaa
    10801 gcagcgattg ccgttgcggt gccagtcgga ttgctcggaa gttacttgta tcagtttcgt
    10861 tttttcatca atactttttt aggtaaatat acagacaaag ctgtagaaga tttgaataca
    10921 aaagctatgg cccgatcaat tattctttat ccaacggttg cctcattttt attattcgtt
    10981 ccgcttgttt tcattgcttt atatttaggg gcgccattga tcgctgatgt aatcaaagct
    11041 ttagagggaa cagtcgttat ccacattctt gaagtagtag gtggagggtt agcggcaatc
    11101 ggtatcgcga caactgtata cgttatcggt cgaaaagatt atctggtctt tttcttcctt
    11161 gcttatttta tgagtgttgt tttgaaatct ttagatatta cgatggttac ttatgccatt
    11221 tttgggatta ttatcgcttt gatctttgtc cagtcgcaaa aagggaaaca agtacagcct
    11281 gccacaagca ataacaattc agcatctttt gatgacgaag atgactatga tgacggattt
    11341 taatttagga ggaatgggaa atggctgaaa taatggataa tcaaacaggt gctcctgaag
    11401 aaatcacgaa aaaagatgta acaaaagcct atctgagatg gcattttgca aacgaaattc
    11461 ctcactcttt tgaacgttac ttggcgccat cactactata cggaatgatg ccaatcttga
    11521 aaaaacttta taaagatgaa gactctttaa aagcagcata taaacggcag ttactgttct
    11581 tcaacactca attaagctgg ggtggcggtg tgattactgg cctaatgtct tctatggaac
    11641 agcaaagagc agaagaagag tatgaagaaa aagagatctt gatgcaggat gacttgatgt
    11701 ataacacgaa agcgggattg atgggggcac tagcaggaat cggagatgca atcgattcag
    11761 gaaccgtcca atatattttt atcgcaatcg cggtaccttg ggcacaacag ggaagtcctt
    11821 taggcgcact ttttcctttt atctgctttg ggttgtatca ggttttacta ggggtatttt
    11881 tcgcaagaca agctttcact atgggccgta atgctacagg cctaatgcaa aacactggtg
    11941 ttcagacagc gattgaaatg ctatcagttt tagggttgtt tatgatgggg gtgcttgcgg
    12001 gaaactatgt aaaagtagaa tcttcattga aatttgcgat atccggacgt gagtttgtta
    12061 tccaggaaat tcttgatcaa atcgtgccag ggatattacc tttagctgtc gtaatgggtg
    12121 tgtacttgtt ctatacgaaa aaaggattga aagtcacaca agcattatta tggctgactg
    12181 gaatattaat tgtgctagca ggaatcggta ttttataaaa agaagggaac gtgaataaga
    12241 tgggtgtagt caatttagcg cgtgtggatg agcgtttgat ccatgggcaa gtaatggtaa
    12301 cgttatctca aaaaagtgga gtgaattcca tttttgttgt agacgaagtt gtagcaaaag
    12361 ataaatttat gagagatctt tacaaaaatg caggaagccg tacaggacaa aaaacaattg
    12421 tcattacacc agagaaagct aaattctatt gggatgaata tcaatttaaa gaatacaatt
    12481 gtattttgat cgcaaaaaca gtccaaacga tttacgactt agtaaaacac ggaatcccca
    12541 tggaagaatt gaatattgga gggattgctc aaaaaaatcc tgatacagat cttttagtga
    12601 cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt gaaagaaatg aatgaacaat
    12661 atggcgtaaa gaatatttat tttcaagcca ctccatctgc acctaaaaca ggattaaaag
    12721 atgtattgaa caagtttgat ttgtcataaa caaatgctta gaaaaaagat aaagagggcg
    12781 aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt tcgttatcgc tatatttacc
    12841 gtaagcgccg tacaaaaaag agtaaacagc gttttctttc agcattagta tcagatattt
    12901 acagtatgag aacagatgtg acagtcattg cttacgatac gctagcctac cgatcaaaaa
    12961 atatttatgt tggagatatc gaaaaagcag agaaagttat ctgtacttat tacgatacac
    13021 ccgttcatgc attaggaagt tatttcatgt ttgattggaa agaccaaagg aaaaaaacaa
    13081 tctactctat attattgtcc ttcattttgt tgttcagttt aggttggtgg ggaatgatga
    13141 tatataacaa aaatcctcat cacgtattcg atctgctatc cgtataaaca ggtatcactg
    13201 tactagcatt tgggagctac tttttcttat tgggaaaagc agctcgcgga tggcctagca
    13261 gacaaacttt tattcgaaat acttcatcca tattaacgat gctagaaatg atccgtacga
    13321 ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg ctgttatgga gaaaagggct
    13381 tagacagcgt gagatcgggt atgaaaaaag aggctattct tttttatcta gactctgttg
    13441 gggcagatac accgctacag ttttcgggaa attatttttc aaataaagaa cagtggctga
    13501 aacaagttga taagttgaaa gaaaaaaacg tcaattatat ttttagtgca agaaaaaagc
    13561 aagcgcagtt cttctatttg accaaaacag atttacgagg gaaaacattt aattggcaaa
    13621 atgccaatca gattattgct ctttttcgat aagtaaaaag tgcaaaacaa ggagtgagaa
    13681 gactaatgtt ggaaatcatg atcgttacac atggtaagtt gagcgaagga ctgaaagatg
    13741 cggcggaagt aattgtcggg acatcgagcc atattgaaac aatgtgctta gagtctgggg
    13801 aggacatcca aacattcgga gagaaggtca aagaaaaatt aactatgatc aacacagcag
    13861 aacccatgct agtatttgtt gatctaatta gcgcaagtcc gtacaatcaa gcgctcttgg
    13921 cagtcaatga actgactgaa gaaaagaaag cagccactta tgtcataggg ggcgtgaatc
    13981 tgccaatgtt gttagaagca atcaatcatc agatgcttca cacaccgata gaagaagcgg
    14041 tggaacaaat tgtcaaacaa ggaaaagata atttaactgt ttggcatgct tcagcggaag
    14101 aaaaaaaatt agatgaggac gatttttaga aggaaggctg aaaagatgaa gtggggattt
    14161 agatggtatg gtgaaaaagg ggattcgatc ccattaaaat atgttcgcca aataccggga
    14221 ataaatggga taattggaac tttgctgact aaaatgcctg gagatgtttg ggagatttca
    14281 gaaattgagg agttgaaagc atctgttgag agccaaggac tggaactgct tggaattgaa
    14341 agtgtagcga tccacgatgc aatcaaggca gggacagatg aacgagatta ctatattgat
    14401 aactatatac gaacaatcaa aaacttgtct cgatgtgggg ttcgattgat ttgttatagc
    14461 ttcaaaccaa tttttggatg ggcgaaaact gatttgttta atgaaaacaa cgatggaagc
    14521 tattctcttt tatacgatca gaaaatcgtc gatcagatgg agccaggaga aatgtatcaa
    14581 ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt gggaagaaga acggttgaaa
    14641 aaattcgctc agctggaaaa actgtatgaa ggaatgaccg aagaagaatt atttgaaaat
    14701 ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa aagcagatgt aaaaatggct
    14761 atccatccag atgatccgcc atgggaactt tttggttttc ctagagtgac gaaaaatctt
    14821 ggcgacttga agaaaattct aaatgcagta gattctccat ataatggact aacgctgtgc
    14881 acaggatctt taggggcaaa tccggaaaac gatttagttg aaatcatcca tgaggtaggt
    14941 tcgcgcatcc attttgtcca cttcagaaac gtggagtttt taggagaaag aaagttcaaa
    15001 gaaacggcac atccctctac agaaggctcc cttgatatgt atcatattat gaaagcgttg
    15061 gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg gtagaaccgt gtggggtgaa
    15121 gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg gactcactta catgcaaggt
    15181 ctatatgagg cgataagtaa ggaaaaacca ctgggagagc attatgaata aaatcaagct
    15241 cttcatcttt gatatggatg gccttttatt agaaactgga cgtttagctt atcgtgctta
    15301 tgtaaaatca gcacaaaagt atgattatga aatgcgtaag gaagtctatt atttactaac
    15361 tggccaaaca gaaatggcga taagagaaca aatgggcatt ttgtacggag aagatgttcc
    15421 atatatcaaa tggcgagaag caatcaatca atataaagaa aaaatcgtga aagaagataa
    15481 gagagtatat acgaaaaaag gagcagaaga aattttatct tttgcaaaag aaagaggaat
    15541 ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta gaaatgtatt taaaaatgga
    15601 aaatctatat gccttgtttg atcaaatcat ttcaggggat gatgtaaaaa aaggaaagcc
    15661 ggaaccagag atctttttga aagcttgttc aaaaatgaat attcctccaa gtgaagcact
    15721 tgtatttgaa gactcgattg cagggatcga agcagcaagg cgagcgggca ttttgtcttt
    15781 tctcatcaaa gatcatttga tagggttgcc cgatcataat gggaagcata agttgaaagt
    15841 agatgtaagt ttatatgaat ccaataaagc agactatgaa ttttatgacc tgcatcaagc
    15901 aaaacgattt ttagaaagta aagggttgta tctctaataa agaggactat tttctagtcc
    15961 tctttattta ttgtcctgca cgactgaggg aacagatgaa aaagaagtaa ttttttattt
    16021 tgaagtatct accttatttg tagtgaacaa tttagaacaa acacaaaacg catctactgt
    16081 tatctaccag tagatgcgtt taaagagaag aacgagaatc gtccatatga gggtttccac
    16141 tccttatgca gattttttta attcttgcac tgcttgttcg atcgtaacac ctgtcgctac
    16201 atgattggta ttttctttgt caaaaacgac aaatagattc aaatctgtat ccaaggttac
    16261 tcgatattgt atatctttgt tatagaatgt catagccatt ctcctttctt taaatacttg
    16321 ctgaaaggca acgaacgcgt tgataacgaa attccattta taagtatacc accaattgaa
    16381 aggttttcat agttaagaaa ttttaaagtc aaaagttgca gataaaagca aataaacaag
    16441 aaaacaaaaa gtgaatcaat cgcggttatt caactttttc ttcgattcga agacaataga
    16501 agacaatgaa ttttatattt cattgcatta tccttgtgtt attagaaaaa gcatgatata
    16561 ttaaatgagt aatctatttt gaaacgtaat ttgagcgata tatctacata gataaaactc
    16621 cttttgccta tcaaatattt attgcaacga aaaaacgtac attgatacgt atcaggagga
    16681 atttatttat gaataacggt acagtaaaat ggttcaacgc agacaaaggt tttggcttta
    16741 tcactggtga agatggaaat gatgtatttg cacatttttc agctatccaa ggtgacggct
    16801 tcaaaacttt agaagaaggc caagcagtgt cttacgatgt tgaagatggt caacgtggcc
    16861 ctcaagcaac aaacattgtt aaagcttaat aaaaattgac ttttaaccgt cttttcgaaa
    16921 gaaaagacgg tttttttgta cgaaaaaatt cattggtcgt tgaatgggct gaatagtagt
    16981 gtataaaaca attttgtgga gcgtgaagct tttatggtag aggaaaatac gaataaagtg
    17041 atgttgataa aaagattcct atcttgaata tgaatcgaga tgtcttgaaa atgaatgagt
    17101 ttgaccaatc attatcctat cgaaatttac agtttcgttc gattggtctg tcaattaatg
    17161 aaaaagtaaa aaagaatatg ttctaggaag agggcggcac atctaaattt ttaagtaacg
    17221 gacagttcaa atatcaagca ttgaaagagg ctatcctact gtttgaagga atacgaatat
    17281 ataaaaacga caaagaggaa aaagtagttc attaaatccc ttcaagtgat gaaagaatga
    17341 gatctaataa aacctgatct ttcatctgtt tttttaataa cgtaataatt tttt
//
LOCUS       DAXWLG010000049        17035 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXWLG010000049 DAXWLG010000000
VERSION     DAXWLG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.049"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(43..588)
                     /locus_tag="ACGE4T_001279"
     CDS             complement(43..588)
                     /locus_tag="ACGE4T_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8386565.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(600..1292)
                     /locus_tag="ACGE4T_001280"
     CDS             complement(600..1292)
                     /locus_tag="ACGE4T_001280"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFF8386566.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1311..1595)
                     /gene="macP"
                     /locus_tag="ACGE4T_001281"
     CDS             complement(1311..1595)
                     /gene="macP"
                     /locus_tag="ACGE4T_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFF8386567.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1608..2171)
                     /locus_tag="ACGE4T_001282"
     CDS             complement(1608..2171)
                     /locus_tag="ACGE4T_001282"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8386568.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2399..3061
                     /locus_tag="ACGE4T_001283"
     CDS             2399..3061
                     /locus_tag="ACGE4T_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFF8386569.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3185..4381
                     /locus_tag="ACGE4T_001284"
     CDS             3185..4381
                     /locus_tag="ACGE4T_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFF8386570.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4482..5858)
                     /locus_tag="ACGE4T_001285"
     CDS             complement(4482..5858)
                     /locus_tag="ACGE4T_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8386571.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5895..6761)
                     /locus_tag="ACGE4T_001286"
     CDS             complement(5895..6761)
                     /locus_tag="ACGE4T_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8386572.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6850..7875)
                     /locus_tag="ACGE4T_001287"
     CDS             complement(6850..7875)
                     /locus_tag="ACGE4T_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8386573.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7893..8567)
                     /locus_tag="ACGE4T_001288"
     CDS             complement(7893..8567)
                     /locus_tag="ACGE4T_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8386574.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8751..9644)
                     /locus_tag="ACGE4T_001289"
     CDS             complement(8751..9644)
                     /locus_tag="ACGE4T_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8386575.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9718..11415)
                     /locus_tag="ACGE4T_001290"
     CDS             complement(9718..11415)
                     /locus_tag="ACGE4T_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8386576.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11408..12721)
                     /locus_tag="ACGE4T_001291"
     CDS             complement(11408..12721)
                     /locus_tag="ACGE4T_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFF8386577.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12721..16990)
                     /locus_tag="ACGE4T_001292"
                     /pseudo
     CDS             complement(12721..16990)
                     /locus_tag="ACGE4T_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         4786 a         3835 c         2954 g         5460 t
ORIGIN      
        1 tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa tttctgcgcc
       61 tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga aacttgccac
      121 ggcatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg ctgtatgcaa
      181 cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc gttctcgcaa
      241 acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta tccaataaac
      301 attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca attcccgtcc
      361 acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa aggaatcttt
      421 tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa acttctttgt
      481 atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca gctttccatc
      541 ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct tagtcctcct
      601 tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg
      661 ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg
      721 atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc ttccatttcg
      781 caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg cgagtcgaca
      841 aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc ttttgctgct
      901 ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat tcctggtaat
      961 tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac tttgtctgat
     1021 ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc tgtattgatc
     1081 accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc taagactttt
     1141 cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga aataaataaa
     1201 gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat ttttacttct
     1261 tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt ctatataaat
     1321 gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga tttcatcaaa
     1381 aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt gattggttta
     1441 ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg ctgataagct
     1501 gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga
     1561 agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta ctttcctttg
     1621 gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat caactgatct
     1681 ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac ttcgtcttca
     1741 tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat atgaaggact
     1801 tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga cttcgcacga
     1861 tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg tccttctcct
     1921 ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt tcgaaattgt
     1981 ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc gccaggatga
     2041 aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc tacagcaaca
     2101 tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc aaattgctca
     2161 ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt ttcatttttc
     2221 aatttttctc aaaaactcct tattagttta ccatagataa accttagatt ttctctaaat
     2281 atagaaaatc cttctttagt ccgatactct ttatagtagt ccgtatgcta aaataagtat
     2341 gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag ggtttattat
     2401 gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct ttgtccgaaa
     2461 caatagtttt tcttttgttc taggactttt attgataggt gtagttttag ttttgtgggg
     2521 actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac tgactgacga
     2581 acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat tttttcgaca
     2641 aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc agcagacagc
     2701 caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag ctttattcaa
     2761 agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt atacgcatct
     2821 gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg agatcaaaaa
     2881 caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt
     2941 aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg acgtagagat
     3001 atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac aaaaggatta
     3061 actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct gtttcgagta
     3121 ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca aggaggaatt
     3181 ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg atacacttga
     3241 agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt cacaacaagc
     3301 ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca aactaccggc
     3361 ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg atgcacagtc
     3421 tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac aatcgatgtt
     3481 gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg aactgatgta
     3541 tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca tcttccagcg
     3601 tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg
     3661 tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc tgctaaaaga
     3721 taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg cactgaatat
     3781 ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca ttccagaagc
     3841 gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca atgattacac
     3901 gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta gacagatcac
     3961 gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa
     4021 gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt
     4081 gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg aaacaacaaa
     4141 cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga
     4201 aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg atttgatcga
     4261 aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga
     4321 agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt taacccaata
     4381 gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac aactgttgat
     4441 gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc attcatcttg
     4501 ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac gataccgaac
     4561 acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa gatggagacg
     4621 attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt gccaaacatt
     4681 tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc catgatattc
     4741 gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc tgccacacca
     4801 gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt cataaactta
     4861 ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata catcatcgag
     4921 atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc cactgttcca
     4981 tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc agtatacgga
     5041 acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa
     5101 ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc tgccattgca
     5161 acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag cacaccggaa
     5221 aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg
     5281 ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa acgtaatgct
     5341 ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac cgcaatcaag
     5401 tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg aagttcggcc
     5461 cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac agaggaaacc
     5521 gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat
     5581 acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc aaattctgcg
     5641 aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag tgacagtgct
     5701 ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt accgatgatc
     5761 gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat ctcactcgta
     5821 tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc ccattttagg
     5881 ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc taaaatgact
     5941 aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc gccaaggatg
     6001 aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa gccaacagct
     6061 aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca taataatcca
     6121 cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc aaagaagctt
     6181 gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc caaattcgcc
     6241 cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa tgctctaaaa
     6301 cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc atcttgtcgg
     6361 gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta tacataatct
     6421 ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa aattaattga
     6481 aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc atggaactgt
     6541 ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa aatccaaggg
     6601 gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc accgatcgtc
     6661 attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa accaatacta
     6721 ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct ccttttattt
     6781 cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt tttcgtttct
     6841 aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata aagtcattgt
     6901 tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt cggccagatt
     6961 ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac catttctccc
     7021 agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga tccctgaatc
     7081 tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga tgtttccgtg
     7141 atccggcgta tatttgatgg cattactaag caattggttg aagataaaag cgatccattt
     7201 agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat ttttttggat
     7261 aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc catattcttg
     7321 gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt gttctacgta
     7381 ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt caatatcgaa
     7441 ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt catggaccca
     7501 tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca ttacttcttg
     7561 gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt ctgtcctccc
     7621 accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt ttccccacca
     7681 tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc cctcaatcaa
     7741 agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc acatcacaaa
     7801 gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat ctttcaaata
     7861 tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc ctgtcctact
     7921 tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa ccgattgatg
     7981 ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc tcgcagcaat
     8041 ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag acgatattca
     8101 tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc aacattcaac
     8161 gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta agaacggcga
     8221 agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt cacaaaatca
     8281 tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct ggagataaaa
     8341 atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc atccaaaacg
     8401 ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc tgagaacact
     8461 tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc ctccatgatc
     8521 aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa aaatccctcc
     8581 atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt ggcttacctg
     8641 gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta atcgcgtttg
     8701 tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta ttatctgtta
     8761 tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc gaaaagcgtt
     8821 agcactagca atatacctag aaggagtata agtaatcgat aaagatggta tttttttgtc
     8881 tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt cactagtaat
     8941 cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc ctgcttttct
     9001 ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac gacttcataa
     9061 gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat ttcttcaaga
     9121 tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga atgtgtactc
     9181 tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc taacaaaaga
     9241 tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata gatggctccg
     9301 atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac cgcttgctca
     9361 aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag cgtctgattt
     9421 ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg ttcaagctgc
     9481 ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc tgctaaaaat
     9541 ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa ttgtaataaa
     9601 agactgattt gggtttgagg tctattacct attttctttt tcattttctt caccttctta
     9661 tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt ttgttgctta
     9721 tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca tcaaacttat
     9781 tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg acccgcgttt
     9841 catattcggt actctttctg gccctggatt ttctactcct acgatctgaa tttcttcatt
     9901 atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg gaagtacata
     9961 accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc cttgaacctc
    10021 aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg cccactcaat
    10081 tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa atattgcgta
    10141 ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt cactgcttga
    10201 tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg gctctactcc
    10261 ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt tgttatggaa
    10321 atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta aatctcctcc
    10381 acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt cttctgtcaa
    10441 aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc cttttgttaa
    10501 aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac caactgtagg
    10561 cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga cagaatcgat
    10621 gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt ctagatcaat
    10681 ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc taaggtcgct
    10741 ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat acaaatgcac
    10801 atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca ccatatcagg
    10861 catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat gagctggcga
    10921 ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt ccgtcacttg
    10981 gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa tacttgtcag
    11041 tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa tcgcttcggc
    11101 agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca aatcgtaata
    11161 tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt caccaaaatc
    11221 cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac cttttttgtg
    11281 taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga ctagttttcc
    11341 gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc ccgtgacaat
    11401 cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca gcacctaaca
    11461 ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc gggaatacgg
    11521 accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt ttgtccgttg
    11581 tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca gagactggaa
    11641 ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat tgtggttggc
    11701 cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt gcttcatcca
    11761 tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa ggcaagaaac
    11821 gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca tttttgtcat
    11881 tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta gcaggagcca
    11941 tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca gcaccttcta
    12001 atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag tatggatctc
    12061 ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg gttgattctg
    12121 tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg atatcaacat
    12181 ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca tcccgatctt
    12241 ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca accgtatctt
    12301 tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt agctgacgga
    12361 tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc atgttttcag
    12421 cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc gctccattta
    12481 gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag atagccaagc
    12541 catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc atccgtttat
    12601 gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca tgtggaagaa
    12661 ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac agctttttca
    12721 ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg ctcctgcaag
    12781 cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc ctgtagcagg
    12841 caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat ttgtctccaa
    12901 aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt ttggataata
    12961 cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat cattttctat
    13021 gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt ttctatagct
    13081 gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg aagcattgaa
    13141 tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat actctcctgc
    13201 actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat tcatactgtc
    13261 aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta cttgtagtac
    13321 accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga tgaccgtatc
    13381 ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat cagatagccg
    13441 atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca gatgcagagg
    13501 ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat agaagtatcc
    13561 gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt atgctcgata
    13621 cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat ctaatgtatg
    13681 gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat tgtaagcagt
    13741 gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt gccaattttc
    13801 ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc gcaagatcca
    13861 tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa tgattttgta
    13921 ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg gctctccgat
    13981 tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat ctactgaata
    14041 gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc ctttcgctac
    14101 tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact ctgcgccatt
    14161 gattggcata ttactgcctt caattagttt attgatattc atgtggattg gcttgaattc
    14221 atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta tgttaggctc
    14281 ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat ctttaccttc
    14341 cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt gacctaacat
    14401 ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat ctactaactg
    14461 ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg ctccagtttc
    14521 atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg tagttttact
    14581 tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg caaattcgac
    14641 tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac ggcctttatg
    14701 ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat cattctcata
    14761 atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc cgtcttgagc
    14821 tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac ctaaagaaat
    14881 cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag tcgccactac
    14941 ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt ggtacttcat
    15001 gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc cagctccaaa
    15061 tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg ttctcttgaa
    15121 ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg cattgtaata
    15181 tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat ttcgtccaat
    15241 agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa tggagaagat
    15301 acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa ctgtgttgat
    15361 cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag aagaaccatc
    15421 accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac gatagtgtac
    15481 atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat gagtaaaccc
    15541 tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt cattatcttc
    15601 agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc tcgtacgata
    15661 acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa cgattaggat
    15721 tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat agccattttt
    15781 aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg tattctcact
    15841 tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga tttgctcatg
    15901 gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa tatccagact
    15961 cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg cagcattttt
    16021 agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat ctagaacaaa
    16081 aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta gatagacatt
    16141 gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg gcagaacagg
    16201 ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt caaaattccg
    16261 ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt cgcctgcagt
    16321 tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt ttgtataatc
    16381 actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt gatagcgttc
    16441 attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa acttcatcta
    16501 ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg gggatctttt
    16561 ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt ccgacatacc
    16621 attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca taggaggata
    16681 gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca tcgattgcta
    16741 aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc cattcaaggg
    16801 tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct aagaatacct
    16861 catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata tgatagactg
    16921 actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc ttcaatttta
    16981 accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca acaat
//
LOCUS       DAXWLG010000050        16612 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXWLG010000050 DAXWLG010000000
VERSION     DAXWLG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.050"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(397..1053)
                     /locus_tag="ACGE4T_001293"
     CDS             complement(397..1053)
                     /locus_tag="ACGE4T_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8386578.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            1246..1524
                     /locus_tag="ACGE4T_001294"
     CDS             1246..1524
                     /locus_tag="ACGE4T_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386579.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1543..2751
                     /locus_tag="ACGE4T_001295"
     CDS             1543..2751
                     /locus_tag="ACGE4T_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8386580.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2899..3264)
                     /locus_tag="ACGE4T_001296"
     CDS             complement(2899..3264)
                     /locus_tag="ACGE4T_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFF8386581.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3411..4184)
                     /locus_tag="ACGE4T_001297"
     CDS             complement(3411..4184)
                     /locus_tag="ACGE4T_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8386582.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4358..5218)
                     /locus_tag="ACGE4T_001298"
     CDS             complement(4358..5218)
                     /locus_tag="ACGE4T_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8386583.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(5218..6636)
                     /locus_tag="ACGE4T_001299"
     CDS             complement(5218..6636)
                     /locus_tag="ACGE4T_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8386584.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6693..7142)
                     /locus_tag="ACGE4T_001300"
     CDS             complement(6693..7142)
                     /locus_tag="ACGE4T_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8386585.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7587..7850)
                     /locus_tag="ACGE4T_001301"
     CDS             complement(7587..7850)
                     /locus_tag="ACGE4T_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFF8386586.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7949..9244)
                     /gene="rho"
                     /locus_tag="ACGE4T_001302"
     CDS             complement(7949..9244)
                     /gene="rho"
                     /locus_tag="ACGE4T_001302"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFF8386587.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(9241..10512)
                     /locus_tag="ACGE4T_001303"
     CDS             complement(9241..10512)
                     /locus_tag="ACGE4T_001303"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8386588.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10677..11546)
                     /locus_tag="ACGE4T_001304"
     CDS             complement(10677..11546)
                     /locus_tag="ACGE4T_001304"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFF8386589.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            11649..11861
                     /locus_tag="ACGE4T_001305"
     CDS             11649..11861
                     /locus_tag="ACGE4T_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386590.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(11838..13448)
                     /locus_tag="ACGE4T_001306"
     CDS             complement(11838..13448)
                     /locus_tag="ACGE4T_001306"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFF8386591.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13670..13915)
                     /locus_tag="ACGE4T_001307"
     CDS             complement(13670..13915)
                     /locus_tag="ACGE4T_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386592.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(14641..15981)
                     /gene="glnA"
                     /locus_tag="ACGE4T_001308"
     CDS             complement(14641..15981)
                     /gene="glnA"
                     /locus_tag="ACGE4T_001308"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFF8386593.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(16009..16401)
                     /locus_tag="ACGE4T_001309"
     CDS             complement(16009..16401)
                     /locus_tag="ACGE4T_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8386594.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4739 a         3527 c         2826 g         5520 t
ORIGIN      
        1 cgtgtcacag ccccttttgt tgcaaataaa cggcgttcaa aagcttacat ctacggtgat
       61 aagtccagcc aagttaaaaa cataagagac tgtttttact tggctcttct tattacttga
      121 tgcagaacag ctttttcaca acctcttggt aaacggtgtt ctcctaccaa ctcctcggtc
      181 acaagtcaca atattcatca aacatgaaaa gctgtttttt gaaattgttc cttgtgactc
      241 agagttaaac ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
      301 tattagctca aaaaacggca aaatatgagg aactattttt ccgtttgttt ctctaatact
      361 caggtcaaaa cgctgatttc acaacctctt tgttcatcaa aagataatat tgtcttctaa
      421 caagtccata ttcaaaataa cgatatgatg gtctttaacg gtgataatac catctttttt
      481 cagttgtttt aaaattctgt ttacactgct agcagttgag atgccacaaa atttggcgat
      541 ttcttcattc gtgatcgaaa aatcgatcag catcccttct tctgtccgca caccgaataa
      601 atcatataat tcgtaaagtt gcgtacaaac agctcccagc tttccgttta atagcatttg
      661 ctgcattttt ttcatcgaat aaagcagacg atagcggtaa tactctttta catagtgttg
      721 tagctttggg tcttggttga tattttgcca aaagcgcaca cgattgattc gataaaactc
      781 tgcatattct gattcaactc gaatattata cggcgaatcg atgaattgtg aatattcgtc
      841 ttttaaaaga gagacgattt caagatcttt gatatagcga aggttaaatt ctctcccatc
      901 atttgaaata acacttgctt ttaccgttcc ctctttcaag atatacgtat gggaatcttc
      961 catcccttcg aatgtcaaat atttatgata tcctttgcgt ataatcggaa aatcattttt
     1021 atccaaatat tcctttaaga ctctactatc caatgatgct cccccatcca aactttcatc
     1081 taaaaagatc atacagtact ttaaaacttt ttaaagtcca aaggtatttt actaatgata
     1141 ttaacatttg ttaagtacct ttttccatca cttatgcgat ttttttcctt ttttttattc
     1201 gttatactgc agaagtaaaa ttttttaaga atagagggaa aaaacatgaa ttactggatg
     1261 aacacaatca ttaatcgatt agaaacagca tatcaaacaa gatttgatat gaaagcttct
     1321 ttagtctttt taaatgatgc gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag
     1381 aatccaacaa acgaatgcga agaattttta aatctgttta tgtctacaag agatttattt
     1441 attcgccaac ttgttgatcg ctatccatcc aattatcacg atgtagaagt tcaaatacaa
     1501 aaattaaaag catattcagc ttaaggggaa tgtataacat acatggaaaa gaaattattt
     1561 aataaatatt ttattggaat cacattgatc aattttattg tctacctgat ttattatctg
     1621 ttgatggtca tcatggcagt cattgcgcaa gacaccttac acgccagctt gagccaagca
     1681 ggttttgctt ctgggatcta tatcatcgga acattgctgg cacgcttatt catggggaaa
     1741 cagttagaac tatttggtcg gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt
     1801 tcaacattag catatcttta tacgccaact atcggggcat tgtatttagt tcgattcttg
     1861 aatggattcg gctacggtac agtttctaca gctacaaatg ctatcgttac tgcatatatc
     1921 cctgcatcta aaaaagggga aggcatcaat tattatggat taagtactag tttagctgcc
     1981 gctgttgggc catttttagg aatgattttg ctgaatatga ctgattttcg tttcattatc
     2041 tggttttcaa ttgtactggt cttctttgtt acgattgcct gccttgtctt tcctgtaaaa
     2101 aatattccat tatctgaaga acaaaaaata tctcttcgct catggaatat caatagtttt
     2161 attgaaaaga aagcactttt cattacagga attgcctttt tgatgggtct ttcttattca
     2221 agtgttttat catttttatc ttcttataca aaagtaatcc acttagttgc tgctgggtca
     2281 tttttcttcg tcgtatatgc tttggtcatc acatttacac gtccattgac tggaaggatt
     2341 ttcgatgtca aaggagaaca atacgtgatg tatcctagct atttattctt aacagcagga
     2401 ttatttttac tgagtgtaac gacgaatagt gtgacattgc ttatctctgg agcattagtc
     2461 ggcctaggtt atggtacgtt tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc
     2521 gaacatcgaa tcagtattgc tttatctacc tactttattg gtttagacct aggcttaggc
     2581 gttggtcctt atttgatggg gatgctaaaa tctcttactt catttagagg attgtatgtg
     2641 attgccggag tcattccatt gatatgtaca gcactctatc ttgttaatgg ccgaaaacat
     2701 agtcatgaag cagagggcga attgattgaa tcattgaaaa atgaaatttg atcttgatga
     2761 atagataaaa aaaaccgttc agatctgaaa ataatctgag cggttttttg agtaaaaata
     2821 gtttatgatt attcttaatt aaacatctat ttatttttaa agctttcatt tatcgataag
     2881 aatagtttaa ctgttgattt attccatttt ccgttcatct ggtacgatgc cttgacttag
     2941 caaccacttg tggaagaacc attctgccac accgatcaat agagcaggaa tcaatacttt
     3001 catccaaata tcgactgtat cattcataaa atatagatag agccacaaga taactgcagc
     3061 tgaaccagca tctgcgatcg tcgctgcgat gttccctact ttacgtaaga tcaacagatc
     3121 ccctagcaca tagatcatga ctacaataat taccccaaca atcgttgaat ccataaagct
     3181 aacaccgtat aaacctgtca gcacgaccca cagaatcgcc cagatcatga ttgccttcac
     3241 taccaacgcc ttcacatgtt tcattttctg cactcctttc tcctggataa aaaagtaaga
     3301 ttatctaaca agataacctt ttcattatta taccatcggc tgagagaagc gagcaaagat
     3361 aaggtcctat ctaaaaatga ggaagccact tagaacattc acgtaataat ttatccgtac
     3421 agacattcga tatggtgttc ctttactttc gcatactgtt cttttgtcag ttctttactt
     3481 gtcaccagat aatcgatttc atgaaaagca atggcttgat gcatgaattt atgtgcaatt
     3541 ttttgctgtg taactaaaac ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc
     3601 attgcctctc cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc
     3661 attatcgcta aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt
     3721 gttgctcgtt tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca
     3781 ctcaacatag cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct
     3841 aggtttctga ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct
     3901 cgaatcaaag aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg
     3961 gtgatcttca tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg
     4021 cgaactctac ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct
     4081 tcgctataat ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga
     4141 taattcacaa tctcctgctg tctattctcc acttcattca acataaaatc accccattta
     4201 ctttatatag taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag
     4261 aagaaatttt tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag
     4321 actattgtca taccctcttg accacacatt tttattttta gtataaacta gccttgccta
     4381 ctgtgttcaa aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg
     4441 gataaattgc attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat
     4501 tgaaaaagac gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg
     4561 cttcgctgac ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga
     4621 tttgttggtt caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat
     4681 acaagccatg cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa
     4741 aaatcttcgc ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac
     4801 cattattccc aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg
     4861 caaccacttt tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca
     4921 tcacggaaga atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat
     4981 ccaagtggat aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa
     5041 actcatcact taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta
     5101 atctctctgc ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg
     5161 gtactgcaaa ttgattctct ttagccactt tcaataactc tttcattgta tataacatta
     5221 cgcttccacc tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt
     5281 cactgttttt ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc
     5341 caagacatac ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc
     5401 atgaggtgcc attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc
     5461 tgtcatcaaa ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc
     5521 tgtaatatac gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt
     5581 aaatttatgt ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc
     5641 agccatacaa gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt
     5701 cgaagtcgat ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa
     5761 tccgaacaat agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa
     5821 cacccatgtg atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga
     5881 taaaaagggg acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt
     5941 atttgccaga aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa
     6001 acctgaacca cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt
     6061 tccagcaatc gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt
     6121 atctcctccg atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc
     6181 agccgctgta atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa
     6241 tggaagcatg taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt
     6301 tgcagctggt tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc
     6361 tattactact tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc
     6421 ggcaaatcgg tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt
     6481 gatttcttgt tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg
     6541 atggtttaga tttcttgctg ttacttctaa tttttctgcc gacatataag tatgagcaat
     6601 tccagtcggg caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca
     6661 ctatcgatac tcggacaaag actctactat tttcattctt gactaatctg tctgatgata
     6721 tctgctggcg tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg
     6781 gagagtgtgc tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca
     6841 aaaatatatt caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga
     6901 aaaaaacaca gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca
     6961 tcttctattc ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga
     7021 acatttctta ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct
     7081 tttgaatcta tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc
     7141 ataaaaaacc tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag
     7201 catcgctagc tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc
     7261 gtgtataagt cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa
     7321 caaaaagcct tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact
     7381 tcgaaataac ccctcttcat ttcgttaata taaggtgttt ttatcaagtg gataattaaa
     7441 actatatact agtttttcat tgttttgtgc attatgatcc tacgactttt tcagcatcaa
     7501 acgaagtttt aaattggcaa aaaacataaa aaaacagact atccctatca gatagtctgc
     7561 taaacgttga gttatcagcg tttttattat tccgcagcgt ttgcgtcttt gagaccgtat
     7621 tttttgttga aacggtccac acgtccgtct gcttgtgtga atttttgacg tccagtgtag
     7681 aatggatgtg agtcagaagt tacttcgaca cggatgactg ggtatgtatt accgtcttcc
     7741 cattcaactg tttcttgtga agtttttgtt gaacctgaca agaatttgaa accagttgtt
     7801 gaatccatga atactactgg gtggtaatct ggatggatgt tttctttcat gattttttcg
     7861 ctcctttgcc ctgctccatt tgctgaacca gagttgattt gtcgatttac aacattaaca
     7921 tcttaacatg tttcagtcaa ggttgcaatt atcttttcag atttctggtc ggattttttt
     7981 taccaaagga gacatcttga aattctttga aaaattgctg attgtttttt gttttacgca
     8041 aaaagcggat cagttgttcc gtatattcta atgaatctcc agtcatattt tttcgaagtt
     8101 tccaaatttc ttctaattct tcacttggca tcaataattc ttcttttcgg gtacctgatt
     8161 ttttgatatc gatggctggg aagatccgac gttcagataa ttcacgtgaa agttgtaatt
     8221 ctagatttcc agttcctttg aattcttcat agatcacgtc atccatacgg cttcctgtat
     8281 cgactaaagc ggtcgctaaa atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc
     8341 cgaaaaattt cttaggttta tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc
     8401 cgcttggtgg aacgactaag ttataggccc gagcaagtct agtaatacta tccatcaaga
     8461 taacgacatc tcgtttatct tcgactaaac gcattgcgcg ttccaatact aattcagaaa
     8521 ctcgagtatg gttttgcggc tgcagatcaa atgtagaaga taccacgtcg cctttcacgc
     8581 ttcgttctag atccgttact tcttccggtc gttcatcaat cagtagaacg attaattcta
     8641 cttccggata gttttcggtg ataccatttg cgatttcttt taatacagaa gttttccctg
     8701 cttttggcgg tgcgacgatc aatccacgct gaccaaaacc gattggagaa aagatatcga
     8761 tcattcgggt agatagtctt tgcggtgttg tttccagttg aagctgtctt tgcggataaa
     8821 gaggtgttaa ggctggaaaa tgcggacgtt cttttgcttc ttccggatct tttccattaa
     8881 cgctttccac gtgcatcaaa ccataataac gttccgattc tttaggtgga cgggcttttc
     8941 ctgccacttt gtccccattt ctcaaactga atcgtctgat ttgtgaggca gaaatataga
     9001 tatcttctgc acttggtcca taattgattg gccgtaaaaa accgtaacca tcttgaccaa
     9061 cgatatccaa tattccttcc atataataaa atccttgttt ctctgcttgt gcacgaatca
     9121 cagctagaga taattccttt ttattcatct tactgtaata aggaatttta aattctttgg
     9181 cataactata aacttctttt aacgttttgt tctctagttc tgccattgtc aaataatcgc
     9241 tcattcagct cccacttcct ctatcatttc aattgatgcc cctaaggctg ttagtttctc
     9301 gataatatga tcataaccgc gtaagatata atctacattg taaatcgtcg ttgtcccttc
     9361 tgccaatagt ccagcaatca ctaaacaagc acctgcccga agatcagaag ccacgacttc
     9421 tgtgccgtga agtttatttg gaccattcac gatgatcata tttccttcca cggtaatatc
     9481 agcgcccatt cgtgctagtt ccggcacatg gttcacacgt ttcgcataga tcgtatcgat
     9541 gatttctgaa gtgcctgtag tcatcaatag taacgcagta agtggctgct gcagatctgt
     9601 ggcaaatcct ggataaggat aggtcatgat gtttgcgggt tttaattctt tcgaggggta
     9661 aacttcgatt tcatcttcgc tgattttcat atggacacca atttcctgca attttgctat
     9721 aaagctttcc aaatgttcaa agatcacatt tttcactttg ataccatttc ctactgcagc
     9781 tgctaatgct agataggttc ctgcttcgat gcggtcagga atcatgaaat gccgacagcc
     9841 atgtaatgtt tcgactcctt cgatacggat cacatctgta cccgctcctc gaacttttgc
     9901 ccccatatta ttcagtaaag ttgccacatc tatgatttcc ggttctcgtg cagcgttttc
     9961 aattactgtc tgtccttttg cttttacagc agctaacatc acattgatcg ttgctccgac
    10021 tgaaaccacg tccatgaaaa tccgattgcc acgcaatcct ttattttctg tccgtaaata
    10081 catggcacca tgttcatttg tcacttcagc acctagagct tcaaatcctt tgacgtgtag
    10141 gtcaatcggt ctagggccta aataacagcc acctggcagg cctactaccg cttcgccaaa
    10201 tttaccaagc agtgttccca taaaataata agaagcacgc aaactattga tttttccgtg
    10261 cggcatcgga atcgatacga cttctcgagg atcgatttct agtatgttgt tggaaaaatg
    10321 gacagttgcg cccatgatct ctaaaatttc aattaatgaa tgaacatctt gtatgtccgg
    10381 cacaccttcc aagatcactg gggaatcagc taagattgca gctggaatca atgcgactgc
    10441 actatttttt gcaccgctga tcgttacttc accagctaaa ggtcggtttc ctttgattac
    10501 gatttttttc atctgtttcc accttgttta ttaatctttt ttcatggata tgcttgtatt
    10561 ttatcataga taaaaaaaag aaacgagcat ggtctgccgt tttaagtaaa aaagaatgga
    10621 gagcgacttc aaaagtcgtt ctccattcct atatatcgta tctgttatgt ggatgcttac
    10681 gctttgttag ctgaaccgaa ccagtcgata tgagatttag catcttcaac gattgctgct
    10741 ttacctggag ctaataattt acgagggtcg aatcctttac cttctttatc tttaccagct
    10801 tcgatatatt tacgagtagc tgcagcaaat gataattgga attcagtgtt aacatttact
    10861 tttgagatac ccattgagat agctttttcg atttgatctt gaggaatacc tgatccgcca
    10921 tgtaatacta aaggcatgtc tggacctaca gcatcagcaa ttgcttgtaa gtggtcaaaa
    10981 gctaatcctt tccagttttc tggatattga ccatggatgt taccgatacc gcaagctaag
    11041 tagtcgatac ctgtagcaac catttgtttg cattcttcaa tatcagctaa ttcgccgtta
    11101 ccgatgattc cgtcttcttc tccgccgatt gaaccaactt cacattcaac tgagatacct
    11161 tttgcatgtg ctttttcaac aacgtcttta gctaatttca agttttcttc aaatggtaga
    11221 tgtgaaccat cgaacattac tgaagtatag cctaattcga tacattccaa agcagcttcg
    11281 taatcaccat ggtctaagtg gatagcaact ggaacagtga tgttcattgc ttcaactaaa
    11341 tcagtgatta aatctttaca gattttgtaa ccacccatgt atttagctgc acccattgat
    11401 gtttggataa gtactggtgc ttgttttgct tcagccgctt caaggattgc ttgagtccat
    11461 tccaagttgt ttgtgttgaa tccgccgaca gcgtaaccgc ctttacgagc agcttttaaa
    11521 aattcagctc ctgatactac tggcataata aaattcctcc taagttttgt aaaactaaat
    11581 ttgttaagac aattcttaac caacgaccct attttaccaa acatttcggt agttttctac
    11641 taaaatcaat gaaaatgaaa attttcaaga agttttttgc aaaaaataaa atatccaata
    11701 gcgctttaat cgaaaaaagt cttttgcttt ctatcttttt tcctacaaat aataacaaat
    11761 cagaaaaaga gaaaagtgac aagaaaattg cttatcccct tctcttgcta cttttctctt
    11821 tctatcaagt tcaaaaatta ttttgtttcg tgatctgcta atgatgctcc gacaaatgct
    11881 ttgattaatt tttgcgggcg atttggacga gaaatcaatt ctgggtggaa ttggcatccc
    11941 acaaagaatt ttttctctgg cagctctacg atttctacta aacggttatc tggtgagaca
    12001 cctgaaaaga ctagcccttg ttcttcaaac aattgacgat atttgttatt gaactcatag
    12061 cggtgacggt gacgttcttg cacaacatcg gctccatcat atgcttcagc cgtcttcgtt
    12121 ccttttttga ttttacatgg gtataatcca aggcgtaaag ttccacccaa attctcgatg
    12181 ttttcctgat cagccattaa atcaataata ttgtttgctg tatctggatt cgtttcagct
    12241 gatgacgcat cttctaatcc aacgacgtta cgtgcaaatt cgacacaagc catctgcata
    12301 ccaagacaaa tacctaaaaa cggaacgtca ttttcgcgag cataacgaat cgcttcgatt
    12361 ttcccttcta ctccgcgatc accaaatcca ccaggaacca agattccatc tgcgtctttc
    12421 aaacgttctt ctacattttc tctcgttaat tcttgtgctt tgatccaatc gatctcgatg
    12481 tctgaattat aggcgaaacc agaatgtttc aatgcttcaa ctactgagat atatgcatct
    12541 ggtaactcga catatttacc gacaagcgca atccgtactt ttttcttcaa gttcaagaca
    12601 cgttcttcta attctttcca ttctgtcatg tctgcttctg gtgtatctag ttttaaatgg
    12661 tcgcaaacaa tttgatccat tccttgtgct tgtaaaacta atgggatgga gtaaagtgta
    12721 tctacatctg gcgactcgat aactgcttct ggttcgacat cacaaaattg tgccaatttg
    12781 tttttcacgc tttgagaaac aggttgctct gtacgtacca ccaaaatatt cggttggata
    12841 cctaaaccac gtagctcttt tacactgtgc tgcgttggtt tagttttcat ttctccagca
    12901 gctttcaagt aaggaatcaa tgttgtatgg atatacataa cattatctgt accgacatct
    12961 gctttcattt gacgaagtgc ttcaaggaat ggcaaagatt ctatatcccc tactgttccc
    13021 cctacttctg taatgatcac atctgaatca gtcattttag ctgctcgcat gattttttct
    13081 ttgatctcat ttgtaatatg aggaataact tgaacagttg ctcccaagta ttcgccctta
    13141 cgttctttac gcaaaacttc agaatagatt tttcctgtcg tcacgttgga gtatttgttc
    13201 aaattgatgt caataaaacg ttcgtagtgg cccagatcca aatctgtttc agcaccatca
    13261 tctgttacga atacttctcc gtgctgatat gggctcatcg tccccggatc cacgttgatg
    13321 tatggatcaa atttttggat agttactttt aagccgcggt ttttcaataa gcgtcctaaa
    13381 gaagctgcaa caatcccttt tccgattgat gaaacgacac cgccggtcac aaaaatatat
    13441 tttgtcataa gttcaaaaac tcctttttaa tttatgtgca ggaaaagtct cagacaataa
    13501 acaaagctcc ctattcttca ataagaatag ggagccgaaa tttctgtaaa cttctgaccc
    13561 tctaaaaggg tgcccaaggt gtataataca agctgactaa gggaaagtca agtactcaaa
    13621 tgaatactat ccgcttacaa ttttttcttt ttcgatatcc tttgagaaat tatttcttaa
    13681 gtcctttatg agtgcaaaaa aaatttaacc cacataataa gcttgatata aacatagcta
    13741 acgttaaacc taagcaaatc agaaaatcgt tactgaaaaa ttttaagtga aataatagat
    13801 aaaaaaataa aacaaacatt atgatagata atatcccacc taacaaagaa ctactttttc
    13861 ctacttgcag caacagttta tatagcttat cgtagaaaca atctttttct ttcaccctta
    13921 tttacctcct ttacgtaaaa aacgtagtcc acagttctcg gatttttggc atatcttttc
    13981 tattaaataa tatcaaaaac aaaacaattc cactaatttt ttcaaagatg accttttttt
    14041 caacaaaagt gccttgtagg gattcattga aggtaacgaa tcacataagt aaaaaataag
    14101 gagatgcgac aaaagtcgtg cctccttatt tctaaataaa cggtgttctc ctgccaactc
    14161 ctcggtcaca agtcacaata tccatcaaac atgagaagct gtttttgaaa ttatttcttg
    14221 tgattcggag ttaaatggta ggctcacaac ctctggataa acggtattca aaagccagtt
    14281 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
    14341 taatactcga aactaaacgt ctttttcaaa acctcttggt aaacggtgtt caaaagcttg
    14401 catctgcggt aataagttca gccaattcaa aaacaagaaa agctgttttt gcttggctat
    14461 tcttattact tgttgcaggg ctgctttttc acaacctctc cactaaacgg tgttcaatca
    14521 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgttagt
    14581 ttctctaata ctcaggtcaa aacgctgatt tcacagcctc taattcacgg tattcctgtt
    14641 ttaataaagt tctagatatt gttctctttc ccactcggaa acagtttgtc tgaatgccgc
    14701 ccattcgaga cgttttgctt caacaaaatt tgagaagata tgttgtccta atgcgtcaat
    14761 cattacatta tccttacgca attctttgat agcattatgg atagttgaag gtaagtcctg
    14821 gatatgtgca gcttctcttt cttcctcatc cattacataa atgttacggt caacagctgc
    14881 tggcggtgtg atctgattac ggataccgtc tagtcctgct tgcaacaata ctgccattgc
    14941 caagtatggg tttgcagatg gatcaactga ccgcaattcc aaacgtgtgg ataaaccgcg
    15001 agattctggt acacgtacta atggcgaacg attacggcca ctccatgcga cataaacagg
    15061 cgcttcataa cctggaacga gacgtttata agaattgact gttggattgc aaacggctgt
    15121 gtaggcacga gcatgtttca gcaaaccacc taagaaatga taagctgttt cacttagttc
    15181 caattcaccg tctttatcat agaaaacatt tccttcatca ttgaacaaag acatattgca
    15241 atgcatacca gaaccattga tcccaaataa cggtttcggc atgaaagtcg cgtgaagtcc
    15301 atgcttgcgt gcaatcgttt tgacaacgag cttgaaggtt tggatattat cacaagcatc
    15361 taccacatct gcatatttaa agtcaatttc atgctgtcct ggcgctactt catgatgtga
    15421 agcttccact tcaaagccta gactttctaa ttccaacacg atgtcacggc ggcaattttc
    15481 acctaaatct gttggtgcaa aatcaaaata accaccttta tcattcaagg tcgttgtgat
    15541 cccaccgtct tcgtctaatt taaataagaa gaattctggt tcaggtccta aattgaaaga
    15601 ggtaaaacca aggtcgcgca tatctttcaa tgctcgtttc aaattcccgc gaggatcgcc
    15661 ggcaaatggt gtcccatccg gattatagat atcacaaatt aaacgagcaa cttttccatg
    15721 agcactttcc cacgggaaga tcatccaagt ggagagatct ggatacaaat acatatcgct
    15781 ttcttcaatt cgaacaaatc cttcaatcga agaaccgtca aacatcattt tattttctaa
    15841 tactttgtcg atctggctca ctggaacttc tacatttttg atcgtaccta agatgtctgt
    15901 gaacatcaat cgtaaaaatc gtacgttttc ttcctcaatg attcgtttga tttcttcacc
    15961 tgttatctgt ttctttacca tggtatccct cttccttttt taatttattc agtcaaagaa
    16021 ttgtctttgc aatccttgcg actgataatg acctgttttg cttaacccac cttgcgccaa
    16081 aatctcatca cgaaaaattt gtcgaacgtc atgatctgtc aacggcttct tgttttttgc
    16141 ttcttgtttt ttcgcctgtg ccatttcgta tacacgtttg atcccagcca tattcaatcc
    16201 ctctgaaaga tagtctttaa tctcaagcaa catatcaata tcattcagcg aatacatacg
    16261 tcggtttcct tcgcttcgtt ccggatgaac taaatcttgt tcttcataat agcgaatttg
    16321 ccgagcagac aaatcagtca gcttcataac tgtaccaatt ggaaatacgg acatagatcg
    16381 gcgcaattcc ttttctccca tcagacgtcc cctttcgttt tataagttgg ctaaagtgta
    16441 ccaattaaaa aaaatattgt caactgcata tgttaggttt tctaacatga gatctctatt
    16501 ttttataaaa taacaaaaaa ataagaacac aaaaaaggta tctagttgat tttcgtacca
    16561 gatacctttt attttttatt atactgtact atcctttatt agtgttttta tt
//
LOCUS       DAXWLG010000051        16485 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXWLG010000051 DAXWLG010000000
VERSION     DAXWLG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.051"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(153..551)
                     /locus_tag="ACGE4T_001310"
     CDS             complement(153..551)
                     /locus_tag="ACGE4T_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFF8386595.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACGE4T_001311"
     CDS             complement(548..1321)
                     /locus_tag="ACGE4T_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8386596.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACGE4T_001312"
     CDS             complement(1318..2001)
                     /locus_tag="ACGE4T_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8386597.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACGE4T_001313"
     CDS             complement(2168..3055)
                     /locus_tag="ACGE4T_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386598.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACGE4T_001314"
     CDS             complement(3052..3921)
                     /locus_tag="ACGE4T_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386599.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACGE4T_001315"
     CDS             complement(4058..5344)
                     /locus_tag="ACGE4T_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8386600.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACGE4T_001316"
     CDS             5617..6195
                     /locus_tag="ACGE4T_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8386601.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACGE4T_001317"
     CDS             6195..7124
                     /locus_tag="ACGE4T_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386602.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACGE4T_001318"
     CDS             7121..8857
                     /locus_tag="ACGE4T_001318"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8386603.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE4T_001319"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE4T_001319"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFF8386604.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACGE4T_001320"
     CDS             complement(9753..10262)
                     /locus_tag="ACGE4T_001320"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFF8386605.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE4T_001321"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE4T_001321"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFF8386606.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACGE4T_001322"
     CDS             complement(11563..12654)
                     /locus_tag="ACGE4T_001322"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFF8386607.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE4T_001323"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE4T_001323"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFF8386608.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE4T_001324"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE4T_001324"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFF8386609.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE4T_001325"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE4T_001325"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFF8386610.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            16290..>16485
                     /locus_tag="ACGE4T_001326"
     CDS             16290..>16485
                     /locus_tag="ACGE4T_001326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386611.1"
                     /translation="MNTNLSKIAKVIRIQFCKFSRFHKKSLQTDVRIVPSIDIKKSTV
                     NHFYETFFNFLIVNFVFQKNN"
BASE COUNT         4767 a         3634 c         2922 g         5162 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taaca
//
LOCUS       DAXWLG010000052        16415 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXWLG010000052 DAXWLG010000000
VERSION     DAXWLG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.052"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            3..1124
                     /locus_tag="ACGE4T_001327"
     CDS             3..1124
                     /locus_tag="ACGE4T_001327"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFF8386612.1"
                     /translation="MVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRINKVPMPS
                     AGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKSIGI
                     LIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAA
                     GISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALF
                     LGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLH
                     HRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAILLFELIG
                     LINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1117..1917
                     /locus_tag="ACGE4T_001328"
     CDS             1117..1917
                     /locus_tag="ACGE4T_001328"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8386613.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1917..2744
                     /locus_tag="ACGE4T_001329"
     CDS             1917..2744
                     /locus_tag="ACGE4T_001329"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8386614.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2749..3456
                     /locus_tag="ACGE4T_001330"
     CDS             2749..3456
                     /locus_tag="ACGE4T_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8386615.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3556..4422
                     /gene="rfbA"
                     /locus_tag="ACGE4T_001331"
     CDS             3556..4422
                     /gene="rfbA"
                     /locus_tag="ACGE4T_001331"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFF8386616.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4436..5008
                     /gene="rfbC"
                     /locus_tag="ACGE4T_001332"
     CDS             4436..5008
                     /gene="rfbC"
                     /locus_tag="ACGE4T_001332"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFF8386617.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5030..6058
                     /gene="rfbB"
                     /locus_tag="ACGE4T_001333"
     CDS             5030..6058
                     /gene="rfbB"
                     /locus_tag="ACGE4T_001333"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFF8386618.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6156..7007
                     /gene="rfbD"
                     /locus_tag="ACGE4T_001334"
     CDS             6156..7007
                     /gene="rfbD"
                     /locus_tag="ACGE4T_001334"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFF8386619.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7042..9075
                     /locus_tag="ACGE4T_001335"
     CDS             7042..9075
                     /locus_tag="ACGE4T_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386620.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKQPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9119..10399
                     /locus_tag="ACGE4T_001336"
     CDS             9119..10399
                     /locus_tag="ACGE4T_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFF8386621.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10609..11415
                     /locus_tag="ACGE4T_001337"
     CDS             10609..11415
                     /locus_tag="ACGE4T_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386622.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11427..12647
                     /locus_tag="ACGE4T_001338"
     CDS             11427..12647
                     /locus_tag="ACGE4T_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386623.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12637..14223
                     /locus_tag="ACGE4T_001339"
     CDS             12637..14223
                     /locus_tag="ACGE4T_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8386624.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14262..16400
                     /locus_tag="ACGE4T_001340"
     CDS             14262..16400
                     /locus_tag="ACGE4T_001340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8386625.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         5206 a         2722 c         3497 g         4990 t
ORIGIN      
        1 gtatggttat aagatttttt ctgacaattt tattatcact gatattaaca cctgtattca
       61 aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag
      121 ttccaatgcc ttcagcgggc ggactgccta tctttatttc atttgttata tcgactttat
      181 tcttattccg taaaattatc cctcaatcgt atattgtacc gatcttgatt gcttctttag
      241 taatcatagt cacaggagtg ctcgatgata aatatgagct gacacctaaa caaaaaagta
      301 tagggatctt gattgcttct cttattattt attttgtagc agatatacgt attgattcct
      361 tcacgcttcc ttttattgga tatatccaac taggctggct tagttttccc gtaacgattt
      421 tttggatctt cggtatcacg aatgcggtca atctgattga cggtttggat gggctggcgg
      481 cagggatttc attgatcggt ttaattacga taggaatcat tggatatttt ttcctccatg
      541 cttctacagt gtatattcca gttgttattt tctgtcttgt agcaagtatt atcggttttt
      601 ttccttacaa tttttatccg gcaaagattt atttaggcga tacaggagct ctattcctag
      661 ggtttatgat ggccgtcctt tcactacaag ggctgaagaa cgtgactttt gtttcatcga
      721 tttctttgct gatcgtcatg ggagtgccgg taacagacac gttttttgct atcattagga
      781 gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa acatcttcat caccgactaa
      841 tcagtttagg ctttactcat aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt
      901 tttctttcac agctatggtc atgaattata ctggaagaat cggaacgatc atattgatta
      961 ttgccatgtt gtttgctgca attttattat ttgaattgat tggtctgatc aatgagaaac
     1021 atcagtttat tttaaaaaca ttgcgatttt tagggaataa agagtaccgt acgcaagtaa
     1081 aacagaaata tttgaagaaa tggaaccgtc atatagatga ataacaaatt aaaccagccg
     1141 atccttatta gtattgtgac ggcaaacagt gataaaattt ttcggacact agataatttt
     1201 atttcaactg taaaagatga aacagcagta aaaatccgaa tttttgacaa caattcactt
     1261 cccgagtaca aaaaaagact aacagagtat aggaaatatc cctacatcga tatctatttt
     1321 catgatgaaa attccggatt tggttatgga cataatcaca atctgatagc tagcgattct
     1381 cgctatggca ttatttgtaa cccagatatt ttagtggatg aagagacagt tacagcattg
     1441 gtcttgttat tgaaagaaca tccggaatgt gcgatgttag cacctaagat tcttaatgaa
     1501 gatggaacaa ctcagcacct catccgtgag cagctggctg tttttgatta cgttttgcgc
     1561 tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac gactggctgt gtttgaatgc
     1621 cgaggattgc ctactgatgc taatagttat gtacggatga tctcaggtag ttttatggta
     1681 gctgatattg ataaatttaa agagataaag gggtttgatg agcgtttctt catgtacttt
     1741 gaagacaacg acttgtgttt gagttttgaa aaagcaggtt acaagttatt gtacacaccc
     1801 ttttatgcag ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc
     1861 gtgtttttga cttctatgat gaaatttttc aataagtggg ggtggcgttt tttctgatga
     1921 aaccagaaat attagtagtc gttgtgcttt atcagcaact attcagtcaa tctccagctt
     1981 atggaccatt aagcaaagca cttagcgaaa agcaaattca gcttattatt tacgataata
     2041 gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat catcatgatc
     2101 cgcaaaatcc tggtttagcg gtggcttata attttgcgtt gtctcaagca gatgaaggaa
     2161 cacgttgtct cgtcactctg gatcaggata cgaggctagt cgacgattat tttgaactat
     2221 tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt tccgatgatt ttttctggga
     2281 gccgacaagt ttctccccta tattcagatc gttatatcaa tcgtacagct caagcagtag
     2341 aagttgaagg cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg
     2401 tcaaatttct aaaaaagatt ggcgggttta atcctgcttt ttctttagat tttttggatc
     2461 attggctgtt ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa
     2521 tggaacatga tctttcggtt ttagactatg aaaaagtttc aagtaatcga taccgttcta
     2581 ttttacaagc agaaaatcga ttttatcatg aatatgatca aaggcattta tccagtcacc
     2641 gacgacaatt attgttaaga acagctaaac agttcttgac cgtcaaaaac cggcatatct
     2701 ggcggatgac tttgcgctca tatgttaaga attggaaggt gtgaattcgt gcgttcggtt
     2761 tgtatagcta cctttaatgg aggcagatat ttaagagagc agttagatag tattttatct
     2821 cagctttcag cagaagatga agtagtcatt tctgatgatg ggtcagtaga tgatacatgg
     2881 gatatattaa ctgaatacgc agaaaaagat aagcgaatcc agcttttttc ggggcctcgt
     2941 caaggattga tcgctaattt tggttatgcg atccagcaga cgaaaggaga tctaatcttt
     3001 ttggcagacc aagatgatgt ttggttggag acaaaagtga aaaagatcac tgactatttt
     3061 gagcagaatc cggatcagct agtagtagtt tctgatttaa tcattgtgga cagcgagctg
     3121 caagagatcc atgattcgta tttttcgttt cgaaattcca aagaaggatg gcttaataat
     3181 gtgataagaa gtcactatat tggtgctggt atggctttcc ggtcttcttt aaaacccgat
     3241 attttgccga tcccgtcaga agtgccgatg catgatatgt ggattggttt gctgggagga
     3301 aagaagactg ggttcctaag agaaccgtta actctgtacc gaagacatga tttaaacgcg
     3361 agtgagattg aaacacattc taaattacgg caaaaaattt cttggcgtgt tcatctgatt
     3421 acggcgttga tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa atccttaata
     3481 tttccatgac aaatataaca attgatgaga taataacaaa gtgaaatttg aaatcaaaaa
     3541 ggagagcaga tatccatgaa gggaatcatt cttgctgggg gcagcggaac acgtctgtac
     3601 cctttaacaa aggcaacatc gaaacaattg atgccgattt atgacaaacc aatgatttat
     3661 tatccaatgt ctactctgat gttggcggga atcaaagaaa ttctgattat ttcaacgcca
     3721 caagatacac cacgtttcaa ggaattattt ggaaacggaa atgatttagg actgcagatc
     3781 gaatatgcag tacaagaaag tccagatgga ttggcacaag cgtttatcat tggagaagaa
     3841 tttattggag atgatagtgt ctgcttggta ttaggggaca atatttacta tggtggcggt
     3901 ctgtctaaaa tgttgcaacg agcagcttca aaagatacag gcgcgacagt atttggctat
     3961 catgtcaatg atccagaacg ctttggtgta gtagaatttg atgagaatat gcaagctttg
     4021 tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg cctttatttt
     4081 tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg tggagaacta
     4141 gagattacgg atgtcaataa agcatatttg gataaaggaa aactatccgt agaacttatg
     4201 ggacgcggat ttgcttggct tgatacagga acacatgaat cattgcttga agcatctacc
     4261 tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga agaaattgct
     4321 taccgtatgg gctacatcga taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa
     4381 aatcaatacg gtcaatattt attgaatttg gccgctgaat aaggagtcct tacagatgaa
     4441 agtaacagat acaaaattaa cagatgtcaa aatcgtagaa atggacgtct ttggcgatca
     4501 tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt gcagaacatg gattgaaata
     4561 tgactttgtt caagacaacc attcactatc agctgaagct ggtgtccttc gggggcttca
     4621 tttccaaaaa ggagaagctg cacagacaaa attgattcgg gtagtcactg gagcagtttt
     4681 agatgtagta gtggatatcc gcaaaggtag cccgacttat ggtcaatggg acggatttat
     4741 cttatctgaa cataatcatc gacagttgct tgtacctaaa ggatatgccc atggattcgt
     4801 tactttgaca ccaaatgtga acttccttta taagtgtgat aattactata atgcagaagc
     4861 agatgggggc atcgcatttg atgatccaga tttagcgatt gattggccaa ttgatccatc
     4921 aagagcgatt atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga
     4981 aaatccattt gtttacggag aaatctaaga attaaggaga aattttctta tgaaaaacat
     5041 cattgttacc ggaggtgccg gattcatcgg ctctaatttc gttcattacg tggtaaacaa
     5101 tcatccagaa gtgcatgtca ctgttttgga taaactgact tatgcaggaa acaaagagaa
     5161 cttggccgga cttcctagcg atcgcgtaga attggtcgta ggagatgttg cagatgcaga
     5221 attagtggac cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg cagaatccca
     5281 taacgataat tcattaaaag atcctttccc atttgtacaa acgaatatca ttggtacgta
     5341 tacactttta gaagcttgcc gcaagtatga tgtacgctat catcacgtat ctactgacga
     5401 ggtttacggc gatcttccat tacgtgaaga cctgccagga catggggaag gcgaaggaga
     5461 aaaattcact gcggaaacac catataatcc ttcaagtcca tattcttcta caaaagctgg
     5521 ttcagatctg ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa
     5581 ctgttcaaac aactatggtc cttatcagca tattgaaaaa tttattcctc gccaagtaac
     5641 gagtatctta agcggtattc gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg
     5701 gatccatacg aatgatcatt cttcagcagt gtggctgatt ttgacgaaag gcagaattgg
     5761 cgaaacctat ctgatcggtg cagacggtga agaagataat aagaccgtga tggaattgat
     5821 cttagaaatg atgggccaac caaaagacgc atacgaccat gtaaacgacc gcgcagggca
     5881 cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa gagctaggtt ggcagcctga
     5941 gtttacgaac ttccgtgatg ggttggctga cacgatcaaa tggtacgaag aacatgaaga
     6001 ctggtggaaa aaagaaaaag aggccgttga agcagcatat gccaaaaatg gacaataata
     6061 cagaggaaat ttcactgtat taatggaaaa acacatgtga ggcaggcgct cgcctgtctc
     6121 ttttttaaaa taaaaatcaa ggagcggata accatatgtt tttattaact ggaggaaatg
     6181 gacaattagg tacagaattg cgccatttat tagatgaaaa aaatctgcca tatgtttcaa
     6241 cagatgcaaa agacttggat atcacagatg ctgagaaaac aatggcttat attacagaat
     6301 taaaacccga tgttattttc cattgcgcag cttacacagc agtagataaa gcagaagaag
     6361 aagcaaaaga attagatgag aaaatcaatg tcgatggcac aagaaatgta gcacttgcag
     6421 caaaagctgt gaatgcacaa ttcgtctata tcagtacaga ctatgttttc gatggaaaaa
     6481 acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc actaaatgaa tatggtcgta
     6541 cgaaattatt aggcgaaaaa gcagtagaag agattttaga agactattat atcatacgaa
     6601 cttcttgggt tttcgggatc tatggacaca atttcgtctt cacgatgcaa cgcctagcgg
     6661 aaacccgcga caaactgact gtagtaaatg accagtttgg gcgaccaact tggacacgta
     6721 ctttggcaga atttatggta tatgtcatcg aagaaaaagc accatttggt atttatcatt
     6781 tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa agaaatattg aaagatacag
     6841 atgtagaagt tgcacctgtt acgtcagaag aatttccgca aaaagcaaca cgtccgcagt
     6901 attcagtcat gagcctgaaa aagacagaag cgttaggttt tgtcatacca acttggcaag
     6961 aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt cctttttatc
     7021 aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag
     7081 ccaaaagcga atcaggcaga ctcgaatttg gtgcttgacc tgtttctaaa actccgctat
     7141 cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt
     7201 ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt
     7261 ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca
     7321 caagctgaag aggacttttc gttggtgaac gaggattatt cattaaacgg acaaaacatg
     7381 atcattgcgt ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg
     7441 ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc
     7501 gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagaggaa aaaagctcta
     7561 tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa
     7621 catgtgggcg atttgatttt ctttactttg ggactgatga ttgcttttta tcattatttc
     7681 tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt
     7741 attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt
     7801 ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat
     7861 gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca tttctggcta
     7921 acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt
     7981 actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct
     8041 tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca
     8101 actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta
     8161 ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg
     8221 ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt
     8281 acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa
     8341 gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc
     8401 tatgcgatta cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt
     8461 gctgtgttgg gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac
     8521 ctcgcttttg tcgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt
     8581 acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat
     8641 cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg
     8701 aaatccttca atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg
     8761 aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag
     8821 gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa
     8881 gacgcagaaa aattagggat cgactatgtg atcagtaaac aaccattaac tgattacaac
     8941 caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt
     9001 tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag
     9061 ctagtcgtac agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt
     9121 gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct
     9181 tttgttgctg acgctagtct tttttgtttg gacagcaggg gtaggttttc cagcaacttt
     9241 gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt
     9301 aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt
     9361 cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt
     9421 tcctttgatt attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg
     9481 cggtatttat ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac
     9541 agagatccaa gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat
     9601 ttggatcaat acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg
     9661 atttgccagt tcttttgaac gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc
     9721 agccttttta ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt
     9781 tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc
     9841 ctacacggtt acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa
     9901 agtcaatttg tttaccggtc gagaagatat ttggcataaa ttctatcaga cgctaggaga
     9961 aaaatctgaa caagttttac taggaatgaa aacatttatc ttccagcgag gaaaccaatt
    10021 tttaggaaac cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg
    10081 gattgctgct ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc
    10141 caacgggcag atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga
    10201 ggatacattg acttcatacc catgggttcc gattatttat atcctgttgg ctatggccgt
    10261 tcatcgatat gatgaaccag aagttacgga ggaattctat gttatggaag aggcatatga
    10321 cgatatgact tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag
    10381 gatccaacgc tatcattgat aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc
    10441 cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat
    10501 cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta
    10561 aaatggcact tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat
    10621 ttgtatttat ttatgaagga catttatcag aacagaaaat tactgctaca gttttcgatc
    10681 aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat
    10741 ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg
    10801 atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc
    10861 ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag
    10921 aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc
    10981 catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca
    11041 gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg
    11101 acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc
    11161 gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca
    11221 ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag
    11281 tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt
    11341 ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt
    11401 tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca
    11461 caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt
    11521 ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa
    11581 aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa
    11641 tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag
    11701 ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt
    11761 taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca
    11821 ttgaatttgc tgacatcggc gaccatattt atcaacctgt aaaaacctat tcaagtggga
    11881 tgttcgttcg tctagctttt gctgtagcga tcaatgtgaa tcctgatatt ttgattgtgg
    11941 atgaagcatt ggcagtagga gatcttgaat ttcaactaaa atgtatggaa aaatttacag
    12001 aaatcaagaa ctcgggaaag acgattttat tcgtttctca tgatattagt tcgattcgcc
    12061 gtttttgtga tcgttcctat tggttgaaaa atggaaaagt tgtcgaatat ggtgatacga
    12121 tggaagtaac gacgaattac gaaaactttt tgaagaaaaa atcagttaaa acagtagatc
    12181 gaaataaaaa ccaagtagaa cgcttcgcag ctccagatat cgtagacgtg acgaaagccg
    12241 aattgttgga taaagataaa aatccagtgg atatgctgga acaaggagaa aaactatacg
    12301 ttaaagtaac ctatgaagta aaagacgata caatcaaaaa tccagtttta ggaattgctc
    12361 tgcgtacagt cgacaatttt tatgtgtgcg gactcaatac tctactagat ggtatcaaga
    12421 tcccttggaa aaaaggtgag aatatctttt atctggagta tgaaaagtta ggtttgttag
    12481 ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat
    12541 acaagacaaa atacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg
    12601 tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac
    12661 ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt
    12721 ttagagtttg gtcctggaaa cggccgatta acgaaacatt tgatcggagc aaaacaatgt
    12781 gaagtgagca ttgtcgagct agataaggaa ttgttcgatt ttgtcagcga gtttgcccaa
    12841 gatggatttt atggagatat cgaaagtttt gaatgggcaa actattatgc tggacaaaca
    12901 tttgattacg ttttgtttgc cgatgtttta gaacatttgg tagatcctgg aaaaacgttg
    12961 aagaaagtta gagaattttt gaatgaagaa ggagaaattc taattacatt ccctaacctt
    13021 gcgcataatt ctgtcatgat tgatttgttc aataatcaat tgccatgggc ttcttatggt
    13081 ttgctagatg aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa
    13141 aaagcaggat tatacatcaa tattgaagat tatttgtact tagctgtagg tgatacagaa
    13201 ttaaaatcaa cttatgaaga attgccggaa gctgtacgtt atgatttcaa aatgcgacca
    13261 tttggcgaag tctaccaata cttcttctca ttgatgaaac atccagtagc acaaagttcc
    13321 attgcgcagc cgcaaaattc taattatgtg aaagtattag aagtaatcca gcaaacgaaa
    13381 caagatgaaa taattcaaca aataccattc aataatttca caggggaaaa tgagacacta
    13441 agtttccatg tatcagaatt agcagaacga atggtgtttc gcttcgcgca gcaacctagc
    13501 tttatcgaat tcagcgcaga agctgctgga gaaaaactca catttatcga ttcaaatgct
    13561 gtagtcaaaa cagtaaatga ttgttatttg tttgatggta aagatttacc agagttcttt
    13621 ttaacggata ttgcagggaa agaagtgact atccattgtc actatcgttt tatcggagaa
    13681 ttgacaccaa caatgaaaga attgctggaa acaatccgtc caatggctga agttacaaag
    13741 cagctttctg agaaaaatga tgaattagaa agagaaaatc attatcttat tgagaataat
    13801 acaagattag atcagacgtt gaaaacaacc ataaatcgct attgcacgtt gttggaaagt
    13861 gatgaatgga caatcaaaca tagattaggg cgacgtaaga aagaaacatc gaaaaaaatc
    13921 caagaaaaag agctttctct atgtattgac gaaaaaatct gggatgcaga aacaaagata
    13981 ttgaaaatca tcgggtgggg aatcgctaat tcggatcgcc aaccattgtc ctataagtta
    14041 tcagccgacc aatcaccatt ttttgaagca attcctgttt tacgagaaga agtcaatcaa
    14101 gcaaaacagc tagctgaagg agcaaaagca ggatttgaac tgcggatcct ttgcgagaag
    14161 gaacgttcat tcttagttga agcagtggct caaaatggtc aatcatggat cattgaaatg
    14221 taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg
    14281 ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct
    14341 gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga
    14401 ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta
    14461 agacccaaag cggttttaag gtacgtttat cagggaaaat gagaggaaaa gcaatcttag
    14521 acttccaaac agcaagacat caaaatggca tagctgtgaa attaaacggt aaatatcctt
    14581 atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa
    14641 attatgcaag gactcatggc gtaaaaaaag caatccgccg ggtcaaactg gaattgaaac
    14701 caggatcgat cgactatgca gagtggatca gtcgtcatga gaaacctgat ctgaaagagc
    14761 aaaggaaaga agtacaagga tttgcctacc gcccattgat ttccatactt atgcctgtct
    14821 ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtacttgat caaacgtatg
    14881 atcactggga actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc
    14941 tagaatccta tcaagcaaaa gatgatagaa ttaaagtcgt tttccgacaa gaaaatggac
    15001 acatcagtct ggcaactaat tctgcattga aaatggcaga aggagagttt atagcgctct
    15061 tagataatga tgacgaatta cctccatttg ccttatatga agtggcaaaa gtgctaaacg
    15121 tacatccaga actggatttg atctacagtg acgaagataa aatcgatgca gatgggaatc
    15181 gctttgaccc gcacttcaaa gcagactggt ctcccgatac attgatggga aataattata
    15241 tctctcactt aggcgtatat cggacaagca ttgttaagga acttggcggt ttccgtaaag
    15301 gatatgaagg ctctcaagat tatgatttgg ttcttcgtgt gacagaacaa attcctgcag
    15361 aacacattta ccacattgac cgtgtcttgt atcattggcg gacgatcccg ggatcaactg
    15421 caagcaatgg agaagctaaa agctacatct atgattctgg agtgaaagca ttgacagatg
    15481 ctctatctag aagaaatatc aagggaagcg tacatcctgg cagaatttct ggtttttatg
    15541 aaatcactta tgatgttttg caagaagact tggtcagtgt gatcattcca actaagaatg
    15601 gctatgaaga tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tatccaaatt
    15661 atgaaatcat tattgcagat aatggaagta cagatcctaa aatgcaagaa ttatttgcag
    15721 aatacaagca tcagctgaaa gaccgattta tcgtcgaatt aattgacatt ccattcaatt
    15781 attcacgtat caacaatctg gcagcagaaa aagcaaacgg caaatacttt ttattcttga
    15841 ataacgatac agaagtgatt gaacctgact ggatgacagc gatggtttct tatgcacagt
    15901 ttgatcgaat cggctgtgta ggagcaaaat tattttatcc ggatgataca acgcagcatg
    15961 caggtgtatt attagggatc ggtggcgttg caggacatgc attgaataac tatgatcgaa
    16021 cacattgtgg ttactttggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag
    16081 cttgtatgat ggtcaaggca gctgacttca atgctgtcaa tggatttgat gaaatattag
    16141 aagtagcatt caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg
    16201 tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata
    16261 caccagaaaa acaaagacgt tttgctgggg aaatcaaaaa aatgcaagat aagtggcctt
    16321 cttatattgc tcatgaccca ttttataacg ataatctaac taaagaagga atcggcgatt
    16381 tttcgttgag acctgattga ttaaaagaac tgtat
//
LOCUS       DAXWLG010000053        15645 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXWLG010000053 DAXWLG010000000
VERSION     DAXWLG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.053"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(77..796)
                     /locus_tag="ACGE4T_001341"
     CDS             complement(77..796)
                     /locus_tag="ACGE4T_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8386626.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(927..2078)
                     /locus_tag="ACGE4T_001342"
     CDS             complement(927..2078)
                     /locus_tag="ACGE4T_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFF8386627.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2295..3035)
                     /locus_tag="ACGE4T_001343"
     CDS             complement(2295..3035)
                     /locus_tag="ACGE4T_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8386628.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3039..3395)
                     /gene="rsfS"
                     /locus_tag="ACGE4T_001344"
     CDS             complement(3039..3395)
                     /gene="rsfS"
                     /locus_tag="ACGE4T_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFF8386629.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(3419..4012)
                     /gene="yqeK"
                     /locus_tag="ACGE4T_001345"
     CDS             complement(3419..4012)
                     /gene="yqeK"
                     /locus_tag="ACGE4T_001345"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFF8386630.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3996..4646)
                     /locus_tag="ACGE4T_001346"
     CDS             complement(3996..4646)
                     /locus_tag="ACGE4T_001346"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFF8386631.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4656..4967)
                     /gene="yhbY"
                     /locus_tag="ACGE4T_001347"
     CDS             complement(4656..4967)
                     /gene="yhbY"
                     /locus_tag="ACGE4T_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFF8386632.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4983..6092)
                     /gene="yqeH"
                     /locus_tag="ACGE4T_001348"
     CDS             complement(4983..6092)
                     /gene="yqeH"
                     /locus_tag="ACGE4T_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFF8386633.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6089..6616)
                     /locus_tag="ACGE4T_001349"
     CDS             complement(6089..6616)
                     /locus_tag="ACGE4T_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFF8386634.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6843..7628)
                     /locus_tag="ACGE4T_001350"
     CDS             complement(6843..7628)
                     /locus_tag="ACGE4T_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFF8386635.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(7621..8490)
                     /gene="accD"
                     /locus_tag="ACGE4T_001351"
     CDS             complement(7621..8490)
                     /gene="accD"
                     /locus_tag="ACGE4T_001351"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFF8386636.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8491..9870)
                     /locus_tag="ACGE4T_001352"
     CDS             complement(8491..9870)
                     /locus_tag="ACGE4T_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFF8386637.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9878..10297)
                     /gene="fabZ"
                     /locus_tag="ACGE4T_001353"
     CDS             complement(9878..10297)
                     /gene="fabZ"
                     /locus_tag="ACGE4T_001353"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8386638.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10322..10798)
                     /gene="accB"
                     /locus_tag="ACGE4T_001354"
     CDS             complement(10322..10798)
                     /gene="accB"
                     /locus_tag="ACGE4T_001354"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFF8386639.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10801..12036)
                     /gene="fabF"
                     /locus_tag="ACGE4T_001355"
     CDS             complement(10801..12036)
                     /gene="fabF"
                     /locus_tag="ACGE4T_001355"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFF8386640.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12055..12792)
                     /gene="fabG"
                     /locus_tag="ACGE4T_001356"
     CDS             complement(12055..12792)
                     /gene="fabG"
                     /locus_tag="ACGE4T_001356"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFF8386641.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12804..13721)
                     /gene="fabD"
                     /locus_tag="ACGE4T_001357"
     CDS             complement(12804..13721)
                     /gene="fabD"
                     /locus_tag="ACGE4T_001357"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8386642.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13828..14052)
                     /locus_tag="ACGE4T_001358"
     CDS             complement(13828..14052)
                     /locus_tag="ACGE4T_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8386643.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14105..15067)
                     /locus_tag="ACGE4T_001359"
     CDS             complement(14105..15067)
                     /locus_tag="ACGE4T_001359"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFF8386644.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15077..15517)
                     /locus_tag="ACGE4T_001360"
     CDS             complement(15077..15517)
                     /locus_tag="ACGE4T_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8386645.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4102 a         3566 c         2684 g         5293 t
ORIGIN      
        1 aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact gaatactttt
       61 cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc tgtacatctt
      121 ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct ggagatagtg
      181 ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg attccttttt
      241 cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg atgatttgtc
      301 cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat tcaaggatct
      361 cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg
      421 ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg
      481 ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt ggtccaaatc
      541 cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt tcgattgctc
      601 ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg aaacgtagtt
      661 tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt tcaatcccaa
      721 attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct acgatatttg
      781 cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat aacgcaaaaa
      841 cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc tttcttcctt
      901 cattcttctc atttttagtc aattatctat actcggattg ggatttgccc gaaattctgt
      961 tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag ttccgcttct
     1021 tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc ttttaagaat
     1081 cttcttccaa catcggtaaa tccaagtagt cgaagataat ttttattttg ttggacagga
     1141 atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat ccgtgtccat
     1201 gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc agttgcagct
     1261 gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt cagttccagc
     1321 aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca agtctgcagc
     1381 gaataagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat tctttttcct
     1441 tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt gatgattttt
     1501 tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg gacatacttg
     1561 gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa atcaagcgta
     1621 atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact atacgtttga
     1681 tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac aaagcgtccg
     1741 aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact gtcacagtcc
     1801 agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg aaccgcccat
     1861 tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt cgcgcgatgc
     1921 catttatcga tgatagccgg ttcgcctcgt tgcaaaaaat tcccgctcat tacagcaatg
     1981 acgacctctg ctcctgttgt ttttcgtgcc atttccacat gatagcgatg tccattgtga
     2041 aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct tcatagttat
     2101 cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact
     2161 atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact gcaagagcaa
     2221 tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct tgttaactcg
     2281 agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga tttctctgat
     2341 gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct gttttccagc
     2401 attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc ttcacgaata
     2461 aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt
     2521 ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg ataatgatag
     2581 ctatattctg gaaaaatttc atctacttga taggtggagt gtacgtcaaa taaaaatcgg
     2641 ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc ttgtctattt
     2701 ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc gatttctgat
     2761 aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata tgctcgctgg
     2821 ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata tccatctttt
     2881 gcaaaggcaa ctgctaatgc tccagtaccg catgccagtt ccaaaatatt ttttgtgtct
     2941 tcttgtaaat gacgtttcga aaaagctagc cattgctgat aaagtgtttc gtccatcact
     3001 tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca ttcactaaga
     3061 tcgactagcg gagcatctga ccatagtttt tccagattat agaaacttcg ttctggtgca
     3121 tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc gccttctttt
     3181 ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat ttcttctgta
     3241 attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc tgccaacaag
     3301 gaaacatttc gaatgtctaa agcgataatg tcttctgccc gttttgaatc tgctgcttca
     3361 accgcaattt ttaattgttc tttactatct atgatgattt cctcctaata tgagcagctt
     3421 atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc tgttgttctt
     3481 gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg tctagatcag
     3541 cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt tcaatatagt
     3601 ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc gtatgcaccc
     3661 ggattgcatg aagaatttct acatcggtga ttcctaattc ccgctctaca aacgaagcgc
     3721 caacaagacc atgccagatt gcattattat aattcaataa ctctggatca aaaccgtctc
     3781 ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg
     3841 cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc acggctgttt
     3901 cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt attttttgca
     3961 tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc atacaaccct
     4021 tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga acagccttct
     4081 ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc tacccaaatt
     4141 acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa gttgaccata
     4201 gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc cccaccgata
     4261 ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt atcataggtg
     4321 taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg attgtcttcc
     4381 acagctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca
     4441 tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt ccggccagct
     4501 tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc caagatccct
     4561 acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg tgaacgaaca
     4621 aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac ttcttttgat
     4681 agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg tccaatgatt
     4741 tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc tgcaatctca
     4801 tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc ttcttcaatt
     4861 tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg ctgaagatga
     4921 tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat tttccttctt
     4981 ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca gcccgcaatt
     5041 actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc tgttttttct
     5101 ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc aggacgagga
     5161 ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc cagtttcgtt
     5221 cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt gccgctgata
     5281 aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag ctgataaact
     5341 ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc taagtagtgc
     5401 gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc gtcatctaaa
     5461 ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt
     5521 actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac atttgttact
     5581 ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa tagttccgtc
     5641 atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat tcctgcttcg
     5701 taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt aggtaatata
     5761 tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg tagtcctggg
     5821 attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc atctttctgc
     5881 cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc ttggatctca
     5941 ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc taaacctttt
     6001 tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg tataacggca
     6061 ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag cccatatcag
     6121 ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta aattgtgtct
     6181 tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct gcacgattgg
     6241 ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca tctgctaaat
     6301 ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct cgtgcaacat
     6361 acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg tttgaaacaa
     6421 caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac tcttctgttc
     6481 catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact gctttgatgc
     6541 ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc catgttggtt
     6601 tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac ataaagtact
     6661 cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac tcgttcgtct
     6721 ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac agaaaaattt
     6781 agaaacaaaa aacaactaag cgagcaagac cattctttac tctttgctta gttgttaaac
     6841 acctaatatt ggcggaacct cttgtatctt ttttccagta attcatgctg gtcgaatcga
     6901 acaagttccg tcaatctttc aatcaacgct tttttcataa tgtgtgcgac tttatatcgt
     6961 ggcaactgct cgccgttgaa cgtttcagga ataactcgat caacgattcc taactcgcgt
     7021 agttctggcg cagtaatttt catcaactca gctgcttctg cggcgcgact accgtctttc
     7081 cacaaaatcg acgcaaatcc ttctggagaa agcacagcat aaatcgtttc ttccagcatc
     7141 cagacttcat ccgctaaacc tagagcaagg gcaccgccac ttccaccttc accaatgatg
     7201 atggcgatga tcggtacctt taaatcagac atttcgagca gatttctggc aatagcctct
     7261 ccttcgccgc gctcttctgc tcctacaccg caatatgcac ctgctgtatt gatcaatgta
     7321 atcaccggtc tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct
     7381 tcaggatggg gagaaccaaa atttctgcgg atattttcct gcagatcccg tcctttttga
     7441 ataccgatca ctgttaccgg tttgccatct aatcgagcga ttccaccaac aatcgcttcg
     7501 tcatctgcaa acaaacgatc accatggcat tcctcgaaag aatcgaagat ctcttcaaaa
     7561 aaatccaagc ttgtccatcg ttctttagct cttgctaact gaacgacatc atgtgctgtc
     7621 ttatccacga tctatccatc ctttctgtgt atgcaattca agaagcgtat ggattttctg
     7681 cttcaattcc atgcggggaa caatctgatc gacaaaacca tgagataata agaattcagc
     7741 cttttggaaa tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc
     7801 aaaaccgatc aagctttggg gttctgctag aatgatatcg ccttccattg caaaactggc
     7861 agttactcct ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac cttcattgct
     7921 atgccgtttg acagcagcag agatttttgc catctgcatt agagaaaaga ttccttcttg
     7981 catccgtgcg ccacctgaag ctgtgaacaa gacgactgga agactttcct ttgttgctcg
     8041 ttcgaataat ctggtgatct tttctccgac aatcgttccc atacttccca tgataaaatt
     8101 aggatccatc actccaataa tgaagggaat cccttgaatg gttgctttac cagtgaggac
     8161 tgcttcatga agtcctgttt tatcttgcat ttttttaatt ttttccgtat attcgggaaa
     8221 tgctagcgga tctttggtta caagatccgt atcccattct tcaaaacttt tttcatcaat
     8281 aatcaaagct agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat gagggcatac
     8341 tttttcttca ccgatgtctt tcgtataaag gatatgtttg caatttggac actttgccca
     8401 catgttatca ggaacagatg gcgagttagc aggtttgctc tctctattag gattgatccg
     8461 aatatatttc tttttcttaa acagagccat ctatgcgcct cctatctccg tttctggtgt
     8521 ccaattcggt aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc
     8581 agggtgacta atcaaatcca tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa
     8641 ttcacttaag gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc catggacgat
     8701 tatttttgca atcattgaat cataatatgg cgggatcgta tatcccgggt aaactgcact
     8761 gtctacccgc agtcccatcc cacctgctgg aagcagtaag ttttggatct tccctggaga
     8821 aggagcaaaa tggaaagcag gattttctgc attaatccgg cattcgattg cgtgtccttg
     8881 gaatactact tcttcttgac acacattcaa aggctcaccg gctgctatct ccacctgttt
     8941 ttttactaaa tcgattcctg tgaccatttc tgttactgga tgttccactt ggattcgagt
     9001 attcatttcc atgaaataaa aatcgccttc ttcatccatc agaaactcga tcgttccggc
     9061 attttcataa ttgactgctt tagctgctcg aacagcagcg tctcctagtg cttctctttt
     9121 tgtttgtgag atcactacag aaggagattc ttccagtact ttttgattat tacgttgcaa
     9181 agaacaatct cgttctccta aatgaatgac attgccaaaa tgatcaccta aaatctgaac
     9241 ttctatatgg cgagcaggat aaatgatttt ttccaaatac atatcatcat ttccaaatgc
     9301 cgctgctgct tcttgctgag ctgaagaaaa atgctgtggg agctcttctt ttgattgaac
     9361 tttccgaatg cctttcccgc caccacctgc agctgctttc agcatcactg gataaccaat
     9421 tctgtctgct atttccaatg cttcttctac tgtagataat acaccagtac ttcctggaat
     9481 gactgggaca tttgcttctt gcatcaattg acgcgcattg atcttatttc ccattgcatc
     9541 gatcgttgca cttttaggac caataaatgt gatattgcac tcttcacaca tggaggcaaa
     9601 tcggctattt tcagataaaa aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat
     9661 tgcagcgctc agtacttcct gaacattcag atatgaatca acggcttttg ccgggccaat
     9721 gcagattgct tcatctgcta actgtgtatg aagtgcttcg gcatctgctt ctgaatatat
     9781 cgctacggtt tggatcccta gttcgcggca tgcacgaatg atacgtacag cgatttcacc
     9841 gcggttagct atcaatactt tagaaaacat atttcaccta tccaatcata aaggttaatt
     9901 ccgcttcggc tactttttta ccgtctactt tagcgattcc tttgcctatt ccggctgaag
     9961 ctttcacttt caatatctca acttcaaggt ataaggtatc tcccggtgta actttcttac
    10021 ggaattttgc tttgtccaat ccgccgaaat aagccgtctt tcccttgaat tgttcaagag
    10081 aaagtaatgc aactgctcct gcttgagcca tcgcttcaac aattaatacc cctggcataa
    10141 caggttcttc tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca tttttcttag
    10201 caacgatccg ttctccttca accatttctt ccactcgatc aatcaaaagc attggataac
    10261 gatgtggaat gatttctttt atttcttgta tgttcattga tttccctctt tcctgctttg
    10321 cttatttttg ctgaatccta aagagtggct gtccatattc gacaactgac tcgttttcta
    10381 caaggatttc tgtgatcact ccgtcggcct cactagttat ctcattcatc agtttcatcg
    10441 cttcaataat gcagataact tctcctttgg caacttgatc acctactttt ttgaaagctg
    10501 gtttgtcggg cgccggttgc agataaacga ttcccacgat tggcgaaaca acctcgattc
    10561 catcaaatac tgccgattct ttttctttca ttgtttctgg agcagttgtt gcttgttcag
    10621 gtatgacatt gactggctcc gaaactggca acgtttcgtt tgtttgaacc atattcatgc
    10681 ctcgacttgt tttattttta ttcatataca attcaaattg accttcacgc aaatcgaatt
    10741 ccgttagcga ggattgatca aattgactga ctaattctct gatttcattg atatccaaaa
    10801 ttattcctcc cagcgtttca tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt
    10861 actcattgca taagtaaaac ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat
    10921 ctcaggatct tgtttttcta cattgatcgt aggtgggata aattgatgtt ctaatgccag
    10981 caaagttgcc aaggcttcga tcccgccggc tgctcccaat aaatgtccag tcatcgattt
    11041 cgtgctgctg acgtaagtat ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc
    11101 ttctgcctgg tcattggctg gtgtacttgt tccgtgagca ttgatatatc cgacttgttc
    11161 cggctgtaca ccagcttctt ccatcgcaag cttcattgct ttagacgcac cagaaccatc
    11221 tggagtagga gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa tgatttctcc
    11281 atagattttg gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta gaacacctgc
    11341 tccttcaccc atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac cacgattagg
    11401 atcagttgat tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat
    11461 cgttgcttct gttcctccag caagaatgac atcagaatag ccatgtttga tgttacggaa
    11521 tgcttcccca attgaattcg ttccgctagc acaagctgtt acgatagctg tacaaatacc
    11581 ttttgcacct acgcgcaaag caatatttcc agctgccatg ttagcaatcg ccattggtac
    11641 gaacattgga gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt ggttttcgat
    11701 tgttggaaga ccaccgatcc cagatccgat gatgacgcca aaccggtctt gattcatttt
    11761 ttctttatct aaaccgctca tctccattgc ttctaaagca gcgtagattc catagatcga
    11821 aaacatatcc attcgtttgc tatctttttt aataaaatat ttatcgtaag ggaaatcttt
    11881 gacttcaccc gctaaggtaa tccctgtttc tgaagcatca aatttagtga tcggtgcgat
    11941 gccattcgtc ccctctttta agctattcaa aaagctgtct gcgtcatttc caataggtga
    12001 agtgactcca tagccagtaa ttaccactcg attcatttgt ttgctctcct tttcttatcc
    12061 gtgcatgacc aatccgccat cgacattgat gacttgtccg gtgatatatg gattttgtgc
    12121 taagaaaact gctgtttgtg caatatcttc tacttgtccg aaacgctgca tcggaatttg
    12181 cttttccgct tgttccttga ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa
    12241 tcctggcgca atggcattac atgtaatgcc tctagttgca gcttctcgtg ccacagattt
    12301 tgttaatcct actacgccag ctttactagc tgcataattt gcctgaccaa tatttccaat
    12361 caacccagat acactagaga gattaatgat ggccccttct cgctgcttca tcattttttt
    12421 caatacgtgc tgcgtcatat tgaaggttcc aatcaaattg atatcaagac attttttgaa
    12481 atcttctgca tccattcgca tcacaagttt gtcatttgtg attccggcat tgtttactaa
    12541 cacatggatc gatccaagct tttcttctgc ttctttgatc atttgtcccg ctttctcata
    12601 atcagagatg tcaccggaaa ctcctacgca ttttacaccg aatgcttcga tttcttctat
    12661 cttttctttt ggaatctctc cgcgaccatt caaaatgata tttgctccag cttttgcaaa
    12721 agctaaggcc atcgctttgc cgattcctct tgtactacct gtgataaata cattttttcc
    12781 ttttacatcc ataacgttcc tccttatagt attgctattg tttcatcaaa tgtctgctta
    12841 ttctcaacac gcagaactgg tattgtttta tcgatttttt tcataaatcc gcttaatact
    12901 tttccggggc cgacttcgat gacttgttcg atgccgattg tttttaactt atggatactc
    12961 tcatagaaac gaacaggttg catgacttgc tgttcaagta gtcccgcgat tgtttctttc
    13021 ttcattattt ccgttgttgt gttgcttatg ataggaaaag aaggttcaga gaattgaatc
    13081 tgacttaggt ccttcgccag tttttttgct gccggttcta aaatagctgt atgaaaaggc
    13141 ccgctgacat ttagagggat cattcttttg aacccttttt cttttaatag taaaacagct
    13201 tcgtctactg ctttttcttc gccaccaatc acgatctgct gaggtgtatt ataatttgca
    13261 ggagatacga tgccgtactt gctagcttca tgacagcttt cctcgatcgt ttcaatcggc
    13321 gcattcatca ctgcaaccat tttcccgctt ccagctgggg cagcttctgt catataagcg
    13381 cctctttttg ctactaacgc aactgcttca gtgaaggata gcgcattact agctactaat
    13441 gccgaatatt ctcctaaact cagtcctaaa gctgcatctg ggatgattcc atgctccttc
    13501 aataagcgat aatacgcgat gctgactgtt agaatagctg gctgtgtata ttgcgtctga
    13561 tccaaacgtt cattttctgt aaagcaaagt tcagccatat cgtagccgag aatctcactt
    13621 gcttcatcaa atgtctgctt tacaacggct tcttcatata aatcttttcc cataccttga
    13681 tattgtgcac cttggccact aaataagaat gctgttttca tgtttttctc caatatatcc
    13741 agaatatatg tttgattctt cctattcaaa aaggccgaga cattcctatt tcaggaaatg
    13801 tcccagcccc caacctaatt ttaatgatta tgcagtttgt ttttctacgt aagtcactaa
    13861 atcttgaacc gttttgattc cgtcttctgt ttcgattttc acgtcaaatt catcttcaat
    13921 ttcattgatg atttggaata aatccaagct gtcagcttct aagtcttctt ggatgtcagt
    13981 agtcaatttg atttcttcag tttcttttcc taattcttct gcgatgattt cttgaatttt
    14041 ttcgaatacc ataataatat attcctccga tattgtttgc tttttatttt tataatttaa
    14101 cagtctacaa ctccatgaga atacttcccc atgttaaacc tccgccatag cctgtaaata
    14161 ttactttctg ctgttttccc aaggtgatct ttccactaga aactgcttca tccaataaga
    14221 tagggatgct tgcagcagat gtattcccat atttatccat attggtcagg aacttttctt
    14281 gcggcatctt tacttttcgg gcaattttct caataattct gaggtttgcc tgatgcaaaa
    14341 gcagataatc aactggtttg tctttcatga cttcttggat attgtttgca acgtctcgta
    14401 cggcaaaatc aaaaatatct ctgccagtca tttgcaaata atacgaacct tctgaatctt
    14461 ctgaatagaa cggattttta ttttgctggt agccagcggt caatgagcta cttctggtac
    14521 cgtcagctgc taatttttct ttcagcaatt ttttctgtgg attcgcttct aagagaatcc
    14581 cacctgctcc atcgccaaac aacacagcag tcgtacgatc agaccaatcg attagcttag
    14641 atagtgtttc accaccaatc actagtccct tagttgagcc gcagcggatc attttttcag
    14701 ccatgcttaa cgcataaaca aatcctgagc acgcagcact gatatcaaaa gcgatagcat
    14761 gagttgctcc caatttccct tgtacttgtg ctgctacaga tggcgtcgtg taatcgggcg
    14821 acattgttgc tactaaaata aaatccaaat ctttagggtt tagatttttc tgttttatca
    14881 atttttccgc cactaaaatg cataaatcag aagtctcctg atttgttaca catcttcgtt
    14941 cacgaatacc cgtccttgac gaaatccatt catcagaggt atccatcatc ttggaaagat
    15001 cattattagt aataatcttt tcaggaagga cgctggctgt tgcagtaatc attccaaatt
    15061 gttccattaa atatcctcac ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat
    15121 ccagcaaggc atgctgttcc tccgtagcca ttccgtctaa gatgttcttg accatttcac
    15181 gatgaaaatg ctgatggacc cgaaatagta actttccttt tttcgttagt cccaacttta
    15241 caacgcggcg gtcatcttca ctgcggatac gttcgacata gccttttttt actaacttgt
    15301 taatagcagt ggtcaaggtc ccaacagtaa ttgatagttc tttggctact tctgaagttg
    15361 tttttttctt gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt gacagatctt
    15421 tgaattgaga ttttttcagc tcagactctt cgattgtcaa aatatcattg aagacactaa
    15481 caagataatc atttactgtt tttaaatctg gttccatgtt ccaccgtcca ctaattagtt
    15541 tgattatcaa atcttttgat gttcaaacta tatcttaact tttttcaaaa tgcaacctta
    15601 aaagatcatt tttttactat atgagctttt tttaagattt tactc
//
LOCUS       DAXWLG010000054        15366 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXWLG010000054 DAXWLG010000000
VERSION     DAXWLG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.054"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            72..2132
                     /locus_tag="ACGE4T_001361"
     CDS             72..2132
                     /locus_tag="ACGE4T_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFF8386646.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2215..2784
                     /locus_tag="ACGE4T_001362"
     CDS             2215..2784
                     /locus_tag="ACGE4T_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386647.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2959..3765)
                     /locus_tag="ACGE4T_001363"
     CDS             complement(2959..3765)
                     /locus_tag="ACGE4T_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386648.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3903..5417
                     /locus_tag="ACGE4T_001364"
     CDS             3903..5417
                     /locus_tag="ACGE4T_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFF8386649.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5820..6803)
                     /locus_tag="ACGE4T_001365"
     CDS             complement(5820..6803)
                     /locus_tag="ACGE4T_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFF8386650.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6808..7455)
                     /locus_tag="ACGE4T_001366"
     CDS             complement(6808..7455)
                     /locus_tag="ACGE4T_001366"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8386651.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7540..8172)
                     /locus_tag="ACGE4T_001367"
     CDS             complement(7540..8172)
                     /locus_tag="ACGE4T_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8386652.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8188..8739)
                     /gene="coaC"
                     /locus_tag="ACGE4T_001368"
     CDS             complement(8188..8739)
                     /gene="coaC"
                     /locus_tag="ACGE4T_001368"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFF8386653.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8736..9473)
                     /locus_tag="ACGE4T_001369"
     CDS             complement(8736..9473)
                     /locus_tag="ACGE4T_001369"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFF8386654.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9470..10459)
                     /locus_tag="ACGE4T_001370"
     CDS             complement(9470..10459)
                     /locus_tag="ACGE4T_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFF8386655.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10717..11259
                     /locus_tag="ACGE4T_001371"
     CDS             10717..11259
                     /locus_tag="ACGE4T_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8386656.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11276..12361
                     /locus_tag="ACGE4T_001372"
     CDS             11276..12361
                     /locus_tag="ACGE4T_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386657.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12373..13179
                     /locus_tag="ACGE4T_001373"
     CDS             12373..13179
                     /locus_tag="ACGE4T_001373"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386658.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13180..14007
                     /locus_tag="ACGE4T_001374"
     CDS             13180..14007
                     /locus_tag="ACGE4T_001374"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386659.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14004..15077
                     /locus_tag="ACGE4T_001375"
     CDS             14004..15077
                     /locus_tag="ACGE4T_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8386660.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4939 a         2777 c         3134 g         4516 t
ORIGIN      
        1 ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag agtagggagg
       61 actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac gcttgtcgtt
      121 tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg cgcacgatgc
      181 tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg acacaaacca
      241 tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact attactcata
      301 ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat aataaagtca
      361 gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta tggacatcgg
      421 aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct acaaaaggtg
      481 atcagttaaa acagatcact gatattttga atcatgcttt gacagatata aaggacggtg
      541 gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa agaaagaaaa
      601 tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg ctgaagaacg
      661 cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat atcgtggata
      721 cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt gctttaaaaa
      781 caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc aaaggaaaag
      841 atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa tatgtaacaa
      901 aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca gtagatacta
      961 caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg
     1021 cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact gtcatcactc
     1081 attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg agtagtgtaa
     1141 atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca aatatagatt
     1201 tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg caagcaaacg
     1261 gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca gaactgatga
     1321 ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata ccagacaatc
     1381 gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac aaagaagagt
     1441 tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat gtatcggtta
     1501 caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt gattttacga
     1561 ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag tcactattac
     1621 atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa gaagaaagct
     1681 tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa gtaaaacaaa
     1741 caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat aaaacgataa
     1801 ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt ttagagccta
     1861 aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat cagaaggaat
     1921 cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc accgatgagc
     1981 atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt ggaggtattc
     2041 cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta tacgctaatc
     2101 gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt atagtcaaat
     2161 aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag aaaaatgtta
     2221 gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca aaatgttcca
     2281 atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga tgaatctgct
     2341 tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc acttgagtat
     2401 tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag
     2461 acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc aagagagcaa
     2521 gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa agggctcgca
     2581 aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga cggaccaatc
     2641 tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa aatgggtttt
     2701 acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa agttatgcag
     2761 gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta ttgggataaa
     2821 aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg gataactaat
     2881 ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac tgccccttct
     2941 cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct tgatgaacgt
     3001 aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa caactgctcc
     3061 aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta agtaaccatt
     3121 ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga ttgtccgagt
     3181 gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac tattgatttg
     3241 ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg aaaattccac
     3301 aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg ctagtaataa
     3361 aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg aaaagccgac
     3421 acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg aaaaatagcc
     3481 ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata acaaaatgat
     3541 ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag ggatttcaaa
     3601 taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc agacctgatc
     3661 cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg tgattctacc
     3721 tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat aactccttca
     3781 tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga cttaaagaaa
     3841 aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa agggggatac
     3901 aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct atgagcgcat
     3961 gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa tctgccgaag
     4021 tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa gaatcttatg
     4081 atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg
     4141 ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac acatctaaat
     4201 catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga atcaaagact
     4261 cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa
     4321 tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat caaaacggta
     4381 tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca catcgtccgt
     4441 ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac gtccatgagc
     4501 gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat ggggaaacgg
     4561 tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa
     4621 tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg
     4681 atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt atcaccatga
     4741 ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat ccaactgtgc
     4801 agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca atcttggtcg
     4861 atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta tctgcagcaa
     4921 ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg
     4981 ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag acaatggaag
     5041 aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt gatggatgga
     5101 atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc atggaacatg
     5161 acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat tacacgatgg
     5221 gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag ccaattaggg
     5281 ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa
     5341 attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca gctcgatttg
     5401 cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga
     5461 gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg aaaaagctgc
     5521 cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt taacttggtt
     5581 ggacttatta ccgcagatgc aagcttttga acaccgttta ttcggaaaaa gagcgtggga
     5641 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacagcg gaagcagaag
     5701 aaattccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
     5761 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttgatc agcatgattt
     5821 tattcagtta tcttcaaaat gatttttccg atgtttttgt ttgcttccat atattcatgc
     5881 gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga ttggccgaat cttgccattt
     5941 tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga gctctgcttt atactgatca
     6001 cttctaggtg tcaataatgt accaagcagt tggacacgtt tcccaatcaa tacaccaagg
     6061 tctatctcag gcacgatggt cccacctagc ataccaatca agacccaacg tccatcaacg
     6121 gctatagaag ctaaattctt ttcccagtaa gaagcgccga tgaaatccaa aataacatcg
     6181 actccccgac cttcagtgat ttttcgtacc tcttcttgaa agtcttgttt tttatagttg
     6241 atcaattcat ctgcaccaag ttctttacaa aaggcgagtt tttcttctga tcctgcagta
     6301 acgatgattt tcgcgtgaga aagctgtcta gctaattgga tcgctgctgt tcccacacca
     6361 cttgctcctg catggatcaa gacagtttcg ccttcttgta attttccaag ccagtaaagt
     6421 gtttgataag cagtcaaaaa aacttctgag attccagcag cagaaacata gtctagcgta
     6481 tcagggagaa ggattgcacg atcggcaggc atcacagcat attcggcata accacctaga
     6541 ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg agatatcact tttattctca
     6601 actacctctc cagctacttc gactcccaaa atagggtagg gaggtttcaa agagactgat
     6661 tctcttttca taatatctgt tcgattgaca gctgctgtat ggactttgat cagtaattcg
     6721 ccttgttttg gtttaggaac aggcacttca atttgttcta atacatctac agaaccagga
     6781 tgcttgattg aaatggcacg catggatcta ttcctccttt gcccattctt ggaatgctgt
     6841 attcaactct ttgctacctt cagaaagtac tagagtcgta cgagaaatat ccgtttcttc
     6901 cactagagcg gcaagtacag cagcaactgt agagcgagtg atcgtttttg atgccttctc
     6961 attaggctta gttaattgaa tggaagtaac ttcttcatcg ttagtcaatg tgactggttg
     7021 aagaatgaca aaatctaagt tagttcgttt cagccattca tctgcataat gtttagtaat
     7081 ataataatct gtcatggact caggccattt tgctggttca tctgcataaa cagcactgac
     7141 catcagatag cgagagacat gcgcattttc agctgcgatc actgttttga ttgcgccatc
     7201 caagtctact tgcagaagat tctttccttg agagccggca gcaaaaatca atacatcgat
     7261 gcctttgaat gcttcggtca ttttttccac ggaccaagtt aaatcaaaag agacatacga
     7321 aacattctct ttttttacta atgtttggga agcaacatct ctcacccctg caaaaatttc
     7381 atgggaactt ggagctaaat cttttatcag gtgtcggcca atttgtccgt ttgcacctac
     7441 aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat ctttccattc aatagtaaca
     7501 ccaaaagaga aaaaaatcag agatcggcta cgatgactat cagttagtaa gagaagggtg
     7561 cttgaagtat cttaacaaga cattgcatac tgctgcagtc gcaagacctg cgacgattcc
     7621 ttcaggtata ccacttgtaa aaataaccgc tagaatagct gaataaacag catttaaatt
     7681 tgttccgatg actttggcat aatcttgctg gaataaaaag tagatcatat tcatcactaa
     7741 aatcgtattg gtcaatgaac cggaaagtcc agctaagaat aaagaaacgg aatctgcttt
     7801 ttgtttcatt agtttgcgca gacctttata aacgaaataa ggaaccaccc caatcaagat
     7861 gcgaggaata aacgcgatca gcaaggcttt ccagcttcca tgttctgttc cgatgactgg
     7921 gataaacgga gaaaagacaa aagacaaagg tgtttggata actgtgctac ggatcatgct
     7981 gatcagtcca aatgtaccgc caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc
     8041 aatgattaca ggaatatgca tggttgttgc attgattgga ccgataggga taaaacctaa
     8101 aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc gcagttaatg taaagttttt
     8161 tgtatttttc ataagtactc cttttttcta agcatttctt tcattaagag tcagcatgac
     8221 ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt ccaaaatctc cacaagctaa
     8281 aagagattct cttggttcaa tttcctgata gccgacagat ctcaatgttt ccagattacg
     8341 ctgagtgatc gggttttgat acatattcgt gttcatggca ggggcaatca attttggtgt
     8401 tcccactgga agcgccattg cgactgttga aagaagatcg tctgcaatgc cgtttgctag
     8461 cttgccgata atgtttgctg tagcaggcgc gatgagaaac aagtcagctt gttttgctag
     8521 ttcgatatga ttgattacct gcggatttga ttcttgcatg acgtctgtat ggactgcgcg
     8581 cttcgacaga gattgcaagg ttaatggcgt gataaaatgt gtgctgcttt tagacataat
     8641 cacatctacc tgggcaccta atttgacgaa ttgacttgta agatcagcgc ttttgtaagc
     8701 agaaatactt ccagtaacgc ctagtaagat ctttttcatt ttgattcctc cagtctaatt
     8761 ttctcagtaa tcaatgcagc aatctcagat ttactttgag cttcttccac tgttccatct
     8821 ttagataaga gatagccgtg gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa
     8881 ataaaatcag ttttgttatt tatcagacta ttttttgcga ctgccagaag agattccttt
     8941 ggtacatcta ctaatagttt gaaaccaatg atttttgctt ttggctgcca ttcacgtaga
     9001 taagaaatga tttttggtgt tttcttcaaa acgagcacaa gatgctcggt gttagaagag
     9061 atctttgaaa cagtggtatc tgtctgttca ttttccgcaa accaattgtc cagttcttct
     9121 tgcgttgaag aagtgggtag gttttttgcc agctgttctt ctgaaaaact aaaagcagga
     9181 gtaaaatcac tgactgccat actgtggata atcgcatcgt agtgttcttt tttcgttagt
     9241 tcttctaatt gtaaaaataa gtccagtgca gattcgatcg tataaatcgt tatattggag
     9301 tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat cgattgtggt atccggattg
     9361 gcagctaaat aatcagctaa tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca
     9421 cgtacttgat cgattttttc agtcgttcct ccagctgtga ttagaatctt catttatggt
     9481 cacctacatt tactagtgtt cttccttggt gtgttccagc aagtagttta gatgctgttt
     9541 caggcaattg atccagagta atctcttgga cgaccagttc atctgcaatc tgcaaatccg
     9601 ccatctgttg ccataaagaa agacgttgat ctattggtac attgacagaa tcaattccaa
     9661 tcaactggat gtttcgcaga ataaaaggca agacagtcgc gtttagtttg attcccccag
     9721 cattgccaca taaagctact gcaccgttat aagagatcaa tggaagaaga ctgcttagct
     9781 gctcgccacc tactgtgtcg atgacatagt cgatttgctg ttttcctaaa gcttttgttg
     9841 tctctggcag aaattcatca ggtcgtacga cttgcaaagc acctagactt tttagccatg
     9901 tgacagactc ttcttttctg gaaaaggcaa tgattgactg gaatcctagt ttatgtaaca
     9961 gggctatcgc tgtactgcct acaccgccag aagcaccagt aacgacaact tttttatcgg
    10021 aggtcatacc ttgacggatt aaagcgttca cacacaacat agcagtaaac cctgctgtac
    10081 caaggatcat cgcttgacgc agcgtcatat tttttggtag aggaactaac cagtctcctg
    10141 gtactttttg atattggcta taaccgcctg gatgggtgac gccaagccca aaaccagtag
    10201 caagtacaag atcttctttt gaaaaacgtg gattgttact ttggacgaca gttccggata
    10261 aatcaatccc tggagtcatc ggatatgttc gtatcacctg accagactcg ctcattgcca
    10321 atgcatcttt ataattgaca tcagaatagg caatttttac caagacttca ttttcagcca
    10381 actcagtaaa tggcagatca ctgattgctg tatggaaaac aggttccttt attacctgaa
    10441 aagatcgaaa agcgttcatt cgtgagacac tccttttaaa attttctttt atcataccat
    10501 aaagcctact cttttattct tgttcttccg taaatttaga caaaaattag gtgaaaaata
    10561 aagaatcaaa caactagaat ttattccaaa agataaagaa atattcagca aaaaaaattt
    10621 tacaaagggt tgcctttttc aataaatttc gtatactaat acgagtggta gtaaacagga
    10681 agtttactat tagaaaactt cgattcggag gaaattatgg aaattggtga gaagttaaga
    10741 aatttgcgca tccaaaagaa tttgacacaa gaagaactag gcgaacgcac agatttgtca
    10801 aaagggtata tttcccagtt ggaacgagat ttaagttcac catcaatgga aacattcttt
    10861 tctattcttg aagtcttagg tgtgactcca gaagaatttt ttcatcaaga agcagcgaat
    10921 ctgcaagtgg tttattcaaa agaagatcat acggtttatt tcgatgaaga aaacgggtat
    10981 gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa tggagcctgt cttgctgact
    11041 tttgaaggtg caggagaata taagacgttt gaaccttcat tatctgaaac gtttattttt
    11101 gtattagaag gggaattaga gttaagctta ggagagacgg tctatgtggc aaaagcagga
    11161 cagtcgatct attatcaagc gacaaaacaa catcaattaa ggaatcatct caaaaaacga
    11221 acaaaagtat tgattgttgc aacgcaatca tatttgtaat atagggggta aaaacgtggg
    11281 gaaaccaatc atttcatttg atcaagtagt gaaacgctat gatgatgaaa cagtattaaa
    11341 aaaagtaagt tttgagatcg aacaaggaaa attttataca ctgcttggtc cttccggctg
    11401 tggtaaaaca acgatcctac gtattatagc tggattcacc gaagctacag aaggggacat
    11461 ctattttgaa gggaaacggt tgaatgattt gccagctaat aagcgacaag taaatacagt
    11521 cttccaagat tatgcacttt ttcctcatat gaatgttttt gataacgttg cttttggatt
    11581 gaagatcaag aaaatgtcca aatcagatat cgaaaagaag gtaaaagatg ctttgcgtat
    11641 ggtccagctt cctggatacg aaaaacggga gattagtgaa atgtctggtg gtcaaagaca
    11701 gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa gtattgctat tagatgaacc
    11761 gttatcggcg cttgatttga aactacgtac agatatgcag tatgaactgc gggatctgca
    11821 acaacgatta ggaatcacgt ttatttttgt tactcatgac caagaagaag cgttggcaat
    11881 gagtgatgag atttttgtca tgaataaagg gaaaatcgtc caaagtggta cacccgtaga
    11941 tatttatgat gagccaatca atcatttcgt tgctgatttt gttggggaga gcaatatcgt
    12001 ggacggtgta atgatcgaag ataatctagt tgagtttgtt ggtaagcaat ttgaatgtgt
    12061 cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt ttacgcccag aggatttgac
    12121 gatcacgaca ccagataaag gaaaactagt cgtaacggtc gatactcagc tgtttcgtgg
    12181 tgtccattat gaaatcatct gttatgatga acagcaaaat gaatggatgg tccattcaac
    12241 gaaaaaagct aaagaaggaa gcaaagtagg attggccttt gagccagaag atatccatgt
    12301 catgcgtttc aacgagtctg aagaagaatt cgatgcacgt cttgacagct acgaagaata
    12361 ggggggacaa gaatgaaaac aatgcgcagg atctattccg tcccttatat tttgtggctc
    12421 ggtttattcg tgatcgcgcc tgtattaatg atcatttatc aatctttttt tgatatgaat
    12481 gggcaattca cgctgaataa ttatctatct tattttacgt cggggacatc gctttctatg
    12541 acgatcaatt ctgtatggta tgcatttttg atcacgtttt tcacgctgat catcagttac
    12601 ccgactgctt attttttaac aaaactgaag cacaagcaat tatggctgat gctgattatt
    12661 ttgcctacat gggtgaatct gctgcttaaa gcttacgcat ttattgggat tttcagtgtg
    12721 cacggcagcg tgaatcattt ctttgatttt atcgggatcg ggccgaaaca gatattattc
    12781 acagacttca gttttctgtt cgtggcaacg tatatcgaga ttccatttat gattatgcca
    12841 attttcaatg cattggaaga gatgaatcct tctttgatca gtgctagtag agatttaggc
    12901 gcgaatagct ttgagacctt ccgacgagtg atttttccgt tatcattaaa tggtgtgaaa
    12961 agtggtgtcc aagcagtatt tattccttct ctttctttat tcatgctgac tcgtctgatt
    13021 ggcggaaatc gggtgatcac tttaggaacg gctatcgaac agcatttctt ggttacacaa
    13081 aactggggga tgggttctac aatcggtgtg attttaatca ttgctatgtt catcgtgatg
    13141 ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa tgaaaaaatt taaatggtcg
    13201 tatctttatt tagggatcgt ctttttattc ttatatgcac cgatcttcta tctgattttt
    13261 tattccttca atgaaggagg aacgatgaac agctttacag gatttacttg ggaaaattat
    13321 gaagctgttt ttgaagatac acgtttgatc attatcgtat tgaatacgtt catgctggct
    13381 ttcttatctg cattgatcgc cacgatcatc gggacatttg gggctatggg aatctattac
    13441 acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga ataacatctt attagtctct
    13501 cctgatgtta ttatcggtgc tagtttcttg attttcttta cactactagg attcggatta
    13561 ggttttcaga gtgtattgct ttcccatatt gctttcagca ttccaatcgt cgtcttgatg
    13621 gtcttgccaa aattgcagga aatgaacgat tcaatggtcg atgcggctag agaccttggt
    13681 gctaataatt tccaagtagt caaaaatatc attttgcctt atctttctcc aggaatcata
    13741 gctggatatt ttatggcatt tacctattct cttgatgatt ttgcggttac atttttcgtc
    13801 acaggaaatg gctttacgac gctctcagta gagatctatt ctcgtgcaag acaaggaatc
    13861 agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt tctccatgat attggtcatt
    13921 ggatactact ttattagtaa agaaaaccgt ccaaaacggc tgaagaaaaa cagaagagta
    13981 cgcgaggggg tggcacggct gcgatgaaaa aattacaatc tctgttttta ggtattctca
    14041 tcattattgc ggtgctgctg cttggtgttc gtcaacttga aaaaacaagt ggcatgagtg
    14101 gagcaaaggt acttaccatc tataactggg gagattatat cgatcctagt ttgatcacga
    14161 aatttgaaaa agaatatgga tacaaagtga attatgaaac atttgattct aatgaagcga
    14221 tgttcacaaa aatccagcaa ggtgggacaa actacgacat cgctatacca agtgaatata
    14281 tgatccaaaa aatgatcaaa gaaaaactag tacttccatt agatcattcg aagatcaaag
    14341 gattaaataa tattgatcca cgatttttag acttggattt tgatccagat aatcagtatt
    14401 cgattccata tttttgggga actttaggga tcgtatataa cgataaattc tttgatgcaa
    14461 atcaaattaa gcattgggat gatttgtgga agccggaatt aaaagacagt ttgatgctga
    14521 ttgacggtgc acgagaagtt atggggcttt ctttgaatag tttaggctat tctttgaata
    14581 gtaagaatat ggcacagctg acagaagcag caaataaatt gaatcattta acaccaaatg
    14641 tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat caatgaagaa gcctccgtgg
    14701 cagtgacatt ttctggcgaa gctgccgata tgatgtggga aaatgaacat ttacattatg
    14761 tgatcccacc agaaggatcc aatctttggt ttgataatat cgttatgcca aaaaccgtga
    14821 aaaataaaga aggtgcctat gatttcatca actttatgct agaaccggaa aatgcggcac
    14881 aaaatgcaga gtatattggt tattctacac caaatgaaga agcaaaagcg ttattgccaa
    14941 aagacatttc gtcggacgaa caattctatc catctgatga cacgatctcc catcttgaag
    15001 tctacaaaga tctaggatcg aaatacctag gcatctataa tgacctattc ttagaattca
    15061 agatgtatag gagataatgg caaaactcag taagaatggg gacttatcaa cgttctaaag
    15121 agctaaaaat cgtccttttt ctatctgagt tttccaatct tctaccaaaa gcggatgaaa
    15181 cgccgctttt tttgtgttat tttatttttt ctaccacgtt tttctaccag agaaaagtgg
    15241 acaaaaccat tgtaaaatag ggcccgagac gggctagtaa atctaagatt gaaaaagtta
    15301 cagaaggata aagtaattgg aaactatgca aatatactta acccttctgt aatctattaa
    15361 taaaac
//
LOCUS       DAXWLG010000055        15076 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXWLG010000055 DAXWLG010000000
VERSION     DAXWLG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.055"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(20..1384)
                     /locus_tag="ACGE4T_001376"
     CDS             complement(20..1384)
                     /locus_tag="ACGE4T_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8386661.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1381..2940)
                     /locus_tag="ACGE4T_001377"
     CDS             complement(1381..2940)
                     /locus_tag="ACGE4T_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386662.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2996..5476)
                     /locus_tag="ACGE4T_001378"
     CDS             complement(2996..5476)
                     /locus_tag="ACGE4T_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386663.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5460..5795)
                     /locus_tag="ACGE4T_001379"
     CDS             complement(5460..5795)
                     /locus_tag="ACGE4T_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386664.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5809..6843)
                     /locus_tag="ACGE4T_001380"
     CDS             complement(5809..6843)
                     /locus_tag="ACGE4T_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFF8386665.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6933..7613)
                     /locus_tag="ACGE4T_001381"
     CDS             complement(6933..7613)
                     /locus_tag="ACGE4T_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8386666.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7897..9000)
                     /locus_tag="ACGE4T_001382"
     CDS             complement(7897..9000)
                     /locus_tag="ACGE4T_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFF8386667.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9629..10099)
                     /locus_tag="ACGE4T_001383"
     CDS             complement(9629..10099)
                     /locus_tag="ACGE4T_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFF8386668.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10429..12069
                     /locus_tag="ACGE4T_001384"
     CDS             10429..12069
                     /locus_tag="ACGE4T_001384"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFF8386669.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12223..13545
                     /locus_tag="ACGE4T_001385"
     CDS             12223..13545
                     /locus_tag="ACGE4T_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFF8386670.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13613..14047)
                     /locus_tag="ACGE4T_001386"
     CDS             complement(13613..14047)
                     /locus_tag="ACGE4T_001386"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386671.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14112..14948)
                     /locus_tag="ACGE4T_001387"
     CDS             complement(14112..14948)
                     /locus_tag="ACGE4T_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8386672.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         4397 a         3048 c         2714 g         4917 t
ORIGIN      
        1 taaaagaccg tgatatttct cacagtcttt tatctactct attgctattt gctctcttag
       61 ctagtacttt aaaagctaac tcattgaggt agtgaaaacg agaggtgtgt tcagacagaa
      121 atccaaaatc ctcaactgct agctgtggaa tcgtagtttt gtgtttcacg atatccgcaa
      181 gcaggttcaa atctgataat aagaattctt ggcttatcag ctgtccgcca attatttcta
      241 atgtatccgg atggtagatc agctttgcat ttactgtttt gttttcttca tcgtattgtg
      301 gaaatagatc aaattctcct gaacacgaac cggtattttg ttccaagaat ggcgcttcat
      361 cttctgtcag ccctacagtt cctaaaaagt agtctataat gactgctgct gttatctttt
      421 gtctgaggtc gtaagcggtt ggctgaccac tcaaagttag cgctactgct cttgccatat
      481 gtacagctcg agtaactaaa ggtaaaaaga gatgccgttt atttttgtca aaactgactg
      541 ggacgatatc tccaatcgca tagatgtccg gatcacttgt ctgcagatat tcattgacta
      601 tcacagtctc atcctcatac aaagccactt tttcttttaa caatgtcgta ttaggacgag
      661 tttgggcaga aaaaatgacc atatctgcct ttattgcttg agatagaagt tgtacggata
      721 ctactttctc ttctgatttt ttccaatcga tcaagaattc atttggataa aaattgattt
      781 gcttagtttg cagctgattt tgaaaactgt tgatcatatc cttatcgaaa tatcttgata
      841 aaacattcgg catataatca actagatgaa taatgtatcc tttatttctc aaagcatctg
      901 ctaattccaa tccaatataa ccagcaccga agatcactac ttcttttgct tcttttaaag
      961 cggcaagtac ttgcttagat tgcactagat ttttataggt gtagacattt tcagcttcaa
     1021 tttgtttata caaggtgaca ggagaggaac ctgttgctac gattaattta tcataggtta
     1081 tggtctcgtt ttttccttct ttggatgcga ggatgagctg atgtttgtct ggatcaagtt
     1141 cagttactct ccattcggtg atcagttttg ccccactttc ttgaagagtc cgcatgttat
     1201 gggcaacttc agctaaacta cgtatctttc cttttaaata aaggttgatt ccatttgaaa
     1261 catagcctag tccatcttta tgatcttcat cgatcagtaa aacttctgct ttcggttgaa
     1321 gttttcttag atttagggca gctgtgatgc ctgcatggga agcaccaatg atcacgattt
     1381 tcatgtttta ttcatcctta aattcaattt ttctaaataa cattctttta aaaattggtt
     1441 caaatagggg aaatcataat tatattcgtt gctagtgaat acaaaaactt tagcggactt
     1501 ctgtgacggg tactctttca attggctagt caaaatgata tcatactgct tttctttctc
     1561 gtattcgact aaatcatatt tgatcgtatt cagcaattgt tcctggatga tttctttcac
     1621 gatatccgcc attagccttt cataaggaag atcataagca atttgcagac gtaattctaa
     1681 ctgcttgatc agcgtccgca aaatagatgc atagcgagca aataattcaa agcgggcatt
     1741 ttcctcgtct acttcagtaa aatcaacaat agcaaatgtt tgtctgacca atctttcaca
     1801 taaaaccagg aattgtttat cttctacttc agttaaccgc tgttttaatc cttcttggcc
     1861 aaaaatattc atccatcctc taaaataggc aaaatgagcg tgattttgca tcactgtcaa
     1921 cgcaatcttt tgcgaaatct cttctggaaa aacataaaaa gagaaatatt cttctatcga
     1981 ttgtttaaac gttcgattca attgagctac tacgtcgtct ttgtattttt cgcttatcaa
     2041 aaactcctgg acatgcgagt tatgtgtatc taatgtaaag ttactgatca tgaaaagata
     2101 aaaaaccaac gtttcatttt gattccaagg aaaggcatat tgaaagaagt aacgggtcaa
     2161 aaatgtatgg aggtccaata aaaattgtgt cgtttgaaac aatccaacag gaagattgct
     2221 ataatctggt ttggattttt tcgtacagcg cattcgcttc ttcataatgg aaagccatat
     2281 ttcaaatttc aatcgtcctt cttctgtaat agtaaaacta aactcttttt gcagcagatc
     2341 taaaaaagtg tttcctgata aatcactgta ttcttttttg ttttcaagat aaggacgtcc
     2401 aaaccaaaat aattggaaat aaaaataaca aatttgactt tctggaccgg tcagccgccc
     2461 gctttttatt ttcagatgaa actcatttaa agaatcattt aattccttca ctctgcgaga
     2521 caaagtagcc gaactgatat taagttccat gacaagtttc tgccgatcaa atgagccatt
     2581 ttccaacaga taaagaagta tctgatattt tgtagaatag cgcaagtagc aatgataata
     2641 aacatcgact gctgctatat ttccatgtct gattaataaa atattccctt cttggataac
     2701 aatttccaat tgtttgatat tttccaagtt agcttgtaaa taaaacagat cttctttcaa
     2761 ggtagcccga tcaactgata agcgggtaag tacgttatta acggaaatct ctcctttttc
     2821 ctctaataaa agttggaaaa gtttgatttg ccgttgttca tttttttcaa gcaaactgaa
     2881 aagttccaag tccactcctc caagcatttc tgattttaac agcagaagtt tccttctcat
     2941 ccttgaaaaa gaggatctgt gacagttatc acagatccat acaaattcta atcacttagc
     3001 tggttggccc agctgtcagt tcccatgtga ccatgccttg atacgttgcg ccaggttgag
     3061 cattcgtact gctatttacc tctaagagta aaccatcggt atcgggaaag tcaacgagaa
     3121 cctctccttt agctgttcca tcactctcat aaatcaacgt gttttgacct tgctgaatca
     3181 attgttttga tccagaatga ttgaagtata attttgttcc gcttaattct tgaccttgat
     3241 cattcttaaa taaagaagtc aatgtccctg tcagttgcca aggagacgtg cttggaagcc
     3301 gagtatcccg cacatcaaaa gaaaaatcgc cttttgcttc ataataactt gtcttcaacg
     3361 gtttggggag atcgttacca aatgtaaaat catctggtac attaactaat gttaattctt
     3421 gtttggggac aagaattgtt ttagcagatg tttggacaac tgggttagga agcttagcac
     3481 catctgctaa tgtaatattg ctggaaagta cagctgcctt tgtcagtatg ctgacggatt
     3541 gtacagaagt atctacagta tttaattgat agttgtaagt aagatgaatg gtatctccta
     3601 ttttgctcaa tagatctgct gggacagaaa ctccttgttg aatgccggta tctgcgatag
     3661 ttgtaccatt taattttaag gaaccattga tataagtagt attttcagga agtgagatcg
     3721 tgattttttt gtcggttatc gcatccccag attttgttaa cgtgaatgtc acattttctt
     3781 gaatcgtatc tcctgataga gcagacaggg gatctcctgt ctcaggttgt ctgctaaggt
     3841 tttttagctg actatctgca ctcacagatg gaaaattagc cactgttatt tgaggaagag
     3901 tggtgattcc agcttgttca gactgccatt tgtacgcaaa agacggatac atgttagtaa
     3961 gtacatcgtc caccaagcga taatcaaaat aatattgccc tgaagccaat gcaggtagtg
     4021 tagaagtagc tgtatttaca cccccgttat ttgattggat cgtttggaaa agttgatctg
     4081 tccatcctgt cgctaatttt tgggttcctt tcgaataaag cctgtaatgg atcgaaccct
     4141 tttttagttc agttgctgac caattggccg tgacttggta cgatgcagaa gaaaggagct
     4201 ttccccactg actatcggct gttaagtttg ttggtaaaac cgttccgtct agcttgtact
     4261 gctccaatac ctttaaagca acaggagaaa gcaatccgcc ttgcgcatct ttcccactgt
     4321 acacgatatt ggctgtctga ttcaatccac ttgtgcttgc tggagtatga gtcagtattg
     4381 ctccttgtgt aacgtttttt cctgtaaaat agttgactac gcgatcaaag cgactaaatg
     4441 gcaactgatc aaaacgtcct tcagtcagcc ccatcgtcaa tgtccgacta ttggtagagc
     4501 ctggaaggtt ctggttattt ccatccacgt attctgtggt atcagcaaat gtattatatt
     4561 ttcctgttga atcacttgtt gagatgtaac tattccacgt atttatctct gcacttgcag
     4621 aaaatgcata tgctcctgga ttcaagtttc cgccagttaa tttaggataa aatctacctg
     4681 cagctggagc taaaatcgaa ttgaacatac gatcttggat aatattattc gaatcataaa
     4741 aatcaacaga aaaaacggcc tttttagaca tagaaggttg taatttaccc ttggtcaaaa
     4801 ttttgtttgg actcgtatca tatttatctc cgttttctgt catcttcgcg acattagctg
     4861 taacatcaaa ttgaagcggg atatcgtcca cgttgaattc gcctcgcgta ataatagcaa
     4921 taatatctgc caatagagta tttgtatccc acgaccccat cccaaaacgc aaggtatggc
     4981 ttcctgctga taataaacta ccgctagtaa ccttgatggg ggcaccggca atatacaagt
     5041 aggcgttgtt ccagtcaatt gcttttaata catcactgct tgatacatgt tccggcaaat
     5101 tgatgatcgc cacaaatgca aatccttgtg cagtgatatt caccaaatca agtacatttg
     5161 ttagtttcac atggctttcc caatgaaaac ggaccgtgcc acctttccaa ggatcatttg
     5221 aaccatcgtt caaataagta gcagtcaatt taggcaattg gttagcccca gttagttgga
     5281 caagcttccc gccattgact gcactacgtc caccatttgg aggtacgata ttttcatacg
     5341 tgtatggatc ataagaaatg gcaggaggcg ttgctccaac agcttctgcc gaaggtgaaa
     5401 agataaatat acttaaaaca aaaagcgaag taaaaaagaa ggagcgaaaa aatgaaaatt
     5461 tattttgtag caacatgttc atcctccttt tcctttcttc tccataacaa caaccacgca
     5521 agaagccata attctatacc aatgatcaaa taaagaaaac ggatcttttc ttcattcgtc
     5581 tccgggaata cgccatgttg attagattct ttataaatcg gtagctgtgt ctgctgtttt
     5641 gatgcgccat tgactgaacc acttggatgc ggaactggac ttagatcata aaatgtaatc
     5701 gtacctgaac ttttggtatc tgcttggact ggctcttgcc agctgaagaa cagcaaactt
     5761 gcaataatcc ctcccaataa gatcgatgtt ttcaacgaaa tcacctcatc atgttgtttc
     5821 tccttttttc tttttcttgt ggacccacaa aaacagcaaa atcaaccata acactagaac
     5881 tgcagcaacc aatcctgcat aaagccatgg gaaagattgt tcttgtactg caaaaacgga
     5941 attttcattt actttttgtg ctttttcttt tttgatcgta aagttttgtg taaatcgcca
     6001 ttcattactg tcgcctgatt ttgctatgat ttctgcgcgg taatcgccgg cttttgcttt
     6061 atcaccagct aaatcaagat tcaagtgaaa attcgaattt ggtgcaacta aaaattcatt
     6121 agacgtattt tcaatataaa gctgattttc ataaaagatc ttgctcgtaa atatcaaatt
     6181 gctgataatc ctaggctgag gattttggaa acgaagctga acagttggct ttgcgttgac
     6241 ctcgtccaaa tctcctgata aaagtttcag atccggttct atcgtctctt gactttcttg
     6301 taaaacgact gcgagattat aagcaatacg atttttgatt tgtgctcctt ctgtagcatt
     6361 ttctgtttca gcttcttctt taggtgaaac atgaataccc gctaatacac gtcccttaaa
     6421 gctttttgct gggattttca cttctatagg tatagttgtt tctgcatgtg ctgatacatt
     6481 gatttccttt ttcgcaatga gcaaaacgtc acggacatca aacggcaatg tctcatctaa
     6541 cgttgggttt ttttgactat aatcgatcgt tccaccatca ctcgttaccg ccggattgac
     6601 agagacctca aatgttcgtt cttcatctga agaattgctg ataattactt ctagtgtctg
     6661 attttgttcc ggtgccacta ataaatcaaa ataaccgatt gctttatcta tttggttctc
     6721 tggaaatacc ggtttgattc caaagtctcc tgctgaatcc tttgcttggc tgattgacgg
     6781 aaaaatgaaa aatagactta aaatagaaaa aaacgaaaca aacagtcgtt tactttgatt
     6841 cattgtccac cacatccttt caacaacaac tgcggtaaaa aatccttcac ctatagtatt
     6901 ttcaccgcag tttgttgaat ttatttcata ctctatgcta ctgggccagc cacgaggttc
     6961 caagtcaaat cagccgtgaa tgttccagct gctgctttcg gcactttcag tgtgatattt
     7021 gctggatcat aaacagaaag ccatgttccc agaccttgat cttttgctgc tgtaaatatt
     7081 ggtgtcgctg cacttgcttt actcaaccca gttacagttt gactagttgg tccagtggat
     7141 tcgttatctg acgtactttt aacagtggat gttccaaaat ctaattcagc gccttgcaaa
     7201 acttgtgaac cgtttacaaa atctgaaacc gaaactgtca cgttccaccc ttgtgcttgg
     7261 ccatctgcac ctacacctcg acgatctgat acttggagat aaggtgtatc gttttttgaa
     7321 gcgtctactt gatacgtttt tgttccgctt tcaatctcgt gtgtaccaaa tggcaattca
     7381 ggaactacat ccaatgttaa agaacctgtc tggccagttg ctggattttc cggatctgct
     7441 ggctctactg gttttgaagg atcagtaggg ttaagaggct caacggtatt tgttggttca
     7501 gtaaaggtaa tatctccttt tgaagtcgct tgaccctcag caaaagctgg tgttgccaag
     7561 ccgcctagaa gaagtgttga aagtaataat gtcccaatca tgttcttttt cataataaat
     7621 cactcctttt tcttagtgac ctaagtatag tagagacagt atgcaaaaaa tcctcatgaa
     7681 ttcatccgct tacttgaaat tatgaccctt tttgtataaa agagaaaaaa acaataaaat
     7741 cgtttacatg aatattcttc cttttttatt aagtatgaaa aaatatttca attttatttt
     7801 ttcgtttcaa attattctat tagtgacatc cttgaactat aaaaatagga gtacggcatc
     7861 atctgccgca ctcctatttt tttcgtcatt tatttcttaa ataaaacgat tctcacgcaa
     7921 ttttattttt ttgatatctt ctactgtttg tgttccagct aattgcatga ccatttccaa
     7981 ttctgtttta aagaaatcaa acacttgatg gactcctgta cttcctccta aagacaatcc
     8041 ataaataacg ggtctgccaa tagcaacaag atcggcacct gatgcgattg ctttgaaaac
     8101 atgttgtcca cgtcgtacac cgctgtcaaa cacgattggt acacgtttat ctactgcttc
     8161 tgccacatat tgcaacgaat caaacgcagc aggcccccca tctaattgac ggccaccatg
     8221 gttagatacc caaattccac cagcaccaga ttctaatgat cgatagacat cttcttctga
     8281 ttgaacacct ttcacgtaga ctggcaaatc agaatgagca gcgataaatt ctacatcttt
     8341 tggactaagt ttttgtttgg aagatttata tacagcatcc atcgtttggc ctactcctga
     8401 ttgataagcc tgaacgatcg gcattggcag cgggaaggta aacccgtttc tgcggtctgt
     8461 ttcacggttt ccgccaactg ttgcatccgc tgtcaatacg attgcttttg ctccattacg
     8521 cttggccact tcaagaatat ccaaattgat tccattatct ttgctcatat aaaattgaaa
     8581 ccattgcggt gctttctccc cgcctgcttc tcttatctct tctaaagtac aagaagcgta
     8641 agaacttgct gtatagatcg tcccaaaatc tgctacacct tttgcagaag ctttctccgc
     8701 cttcacatga gctaatccat gagcagcaac tggtgccatg atgattggtg cagtcagcat
     8761 ttcttcgtca aaatgtgtag ttgtatcggg taattctaca tctcttaata catgaggaat
     8821 aatcaaacga tgattgaatg cccgttcatt ttcttgataa gtaaataagt cgccagcacc
     8881 actgctgatg taaccataac caccagctgg aatcacttgt gaagccgcga tttctaaatc
     8941 ttccatattg ataaaatcta ctattccttc atttgtacct gcttgatatg ttttttccat
     9001 ctcaatgcct tctctctata aaatttgaat gatgaacaat atgatagcgt ttacactttt
     9061 tatattactc ttcgacattg atacaggcaa ataatctgtt tacatttcag ccattccaga
     9121 tgtcactact ctggaagaaa tgattgtaat aaaaatagat aaaaaaccga tgggactttg
     9181 acaaaagtag tgtcaaagtc cctttttttg aataaacggt gttcaaaagc cattttctcg
     9241 gtattaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttacta
     9301 ctcgaaacta agcgactttt tcacaacctc tcgataaaac ggtgttcaat cagctgaccc
     9361 tcggtattag ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa
     9421 tactcaggtc agaacgttga cttcacagtc tcttccttaa acggtgttca aaaacttgca
     9481 tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
     9541 ttattacttg atgcagggca ggtttttcac gacctctgat tcacggtgtt tctttttcat
     9601 caacattttt acgaaatact gtggaagact attgaaataa gtgtggattg tttctttgtg
     9661 tttcaatcgt ttcttgccat tcttcattta attttggatt accgttttct tccttgaact
     9721 cataaggtcc taattcaaaa atcccagaca gtatttgatt cacgatttca tatagttcag
     9781 tgtgatcaga cacatgattc ttgaagatca tctcattatc accacgtttg aacctcacat
     9841 tcgggctctc atgatgaacc tctatgcatt tttctccttt ttttaactga cggacttctg
     9901 aaaacatctc ataaatatct tctccagtaa aaccttcagc aagcgccaga ctgtccacaa
     9961 gaccgcctac tgtcagttca gagattattg cttgattgga atctaatttt aatgtatagc
    10021 ttgtcgcttc tactacgcca catgcattgc aaccgccttc tgatagttcg ctttttctaa
    10081 tcaattgaac ttctttcatt ttctcttcct cctcttaatt ttctacttat gtatttttat
    10141 cacctgacat aggtgactcc tatcaacgct atcatgtgct tttttcctca gaaatcctat
    10201 ttatccattc catctatccc ttattcttct acttttcttt ctctagcata taagagatac
    10261 gcatagtatt caagcaagga ttgtcgatat aaaaagaaca aatactgcta ttttgatttc
    10321 ttttggctat attacatata aataaaattt atcatacctg ctctttttga ctatatcatc
    10381 atctgacttc tatacttcgt ttgtattaaa acttaggagg aatcattcat gcttacaggg
    10441 ttaaacttat taaataatcc ttttctaaat aaaggaacag cttttacaaa agaagaacgg
    10501 gcaaaatatg gaatcactgg tatgcttcca agtactgtcc aaacactgga acaacaatct
    10561 gttcaagctt atggtcaata cttaagcaaa caaacagatt tggaaaaacg tatcttctta
    10621 atgaatctat tcaatacgaa tcggacattg ttttataaat tgatgggcga acatttagtt
    10681 gaattcatgc ctgtcgttta tgatcctgtc gttgcggatt caatcgaaca atacaacgaa
    10741 attttcttga aaccgcaaga tgctgcattt ctttcaatcg acgaaccaga aaacatcaaa
    10801 gcaagtttga agaacgcggc agatggacga gacattcgtt tgattgttgt gactgatgca
    10861 gaaggaatct taggtatggg cgactggggt gtgaacggtg ttgacatcgc tatcggcaaa
    10921 ttgatggtct atactgctgc tgctggtatt aatccagcac aagttttgcc tgtatcgatc
    10981 gacgctggta ctaataatga agaattattg aataatccct tatacttagg caatcgtcat
    11041 gctcgtgtag aaggcgaaac ttactatgaa ttcatcgatc aattcgttca atcagctacc
    11101 gaattattcc cagaattgtt attgcattgg gaagattttg gacgaggaaa tgctgcagcg
    11161 atcttagaaa aatacgaaga tcaaatcact actttcaacg acgatatcca aggtacagga
    11221 attgttgtcc tagcaggtgt attaggcgga ttgaatatat ccggtgaaca attgaaagat
    11281 caaataattt tgacatttgg tgctggtact gctggtgtgg gtatcgcaaa catcctttta
    11341 gatgagatga tccgtcaagg tgtacctgaa gaagaagcac gccaacattt ctatcaagta
    11401 gataaacaag gtttattgtt tgaagataca gaaggattga ctcctggtca aatcccattc
    11461 gctcgcaaac gctcagattt cactaatgct gatgaattga caaacttaga agcagttgta
    11521 aaagcgatcc atccgacaat catgattggt acttctaccc aacctggagc attcactgaa
    11581 gaaatcgtga aagaaatggc tgctcataca ccacgtccga tcatcatgcc tttgtctaat
    11641 ccaacaaaat tggcagaagc aaaagcaaaa gatttaatag aatggacgga cggaaaagca
    11701 ttagttggaa caggtatccc tgctgctgat gttgaatata atggtgtaac ttaccaaatc
    11761 ggtcaagcaa ataatgcttt gatgtaccca ggtttaggac ttggtttgat tgcttcaaca
    11821 gctacacgag taaatgctga aatcatttct caagctagtc gagcattagg cggaatcgtt
    11881 gatgtaacaa aaccaggagc agctatcttg cctccagtag caaaaatcac tgaattttct
    11941 caaacgattg ctgaaactgt cgcaaaatca gttgtcgcac aaaacttgaa tcgtgaagaa
    12001 atcacggata tcaaagaagc agttgaatct gctaaatggg ttcctgaata taaatcttta
    12061 gaagattaag atcgttatag taaaagaaca tacagaaact ttgtatgacg tctaaaaaga
    12121 aaaaagcgac tgcggcaaaa cagcggccag ccaactcctg ctcttttgtc gcttttcttt
    12181 ggcttttttc atatgataca gaataagaaa ggatctgtaa aaatggcaca aaaaacaaca
    12241 actgcaactg cagttgagga aaagaaaaaa gaaggttttt ggtccacaaa aatcaccggc
    12301 gtaagtttgc cactttattt gatcatggtc cttgtcctga tcattgcaat ggctttaggg
    12361 aaattaccga cgaatatgat tggaccaatc gcagttttgg ttattttcgg taatttattt
    12421 cattttatcg ggacaaaaat ccctatcgtc aaaagttatt tgggtggtgg ttccgttttt
    12481 gcgatcttcg cttctgctgc gattgctaca tttgggatct tacctgaata tgtagtgaaa
    12541 tccacagaaa actttgtcag caatatgggc ttttgggact tttatattgc tgccttgatt
    12601 actggtagca tccttggtat gaaccgtaat ctattgatga aagcttctgt tcgttttatt
    12661 cccgttgcac ttatttcaat ggtcgtctcc ttttttgccg taggactcgt gggcatgctt
    12721 atcggaaatg gttttgcaaa ttccgtttta tacatctcat tacctgcgat ggctggtggt
    12781 gttggagccg gagccgttcc tttatcaacg atttatgcaa atgttttagg tacggatgct
    12841 tcttctatca tttcccgcct gattcctgcg acagcaatga caaatgtctt agctatcatc
    12901 ggtgcagcat tagttgctcg tgctggacaa tcaatgccaa aattcaacgg caatggtaaa
    12961 ttaatggaaa aaggcgatct tggcgatgga aaacctcgaa ctgtgaaacc ttccattcca
    13021 cagttaggtg ttggtttgat ggtatcattg actttcttca tcttaggaca aattggtaac
    13081 tattttgttc ctaaagtcca tgcttacgca tttatgatta ttatcgttgt catctgtaaa
    13141 atcgccaacc tattaccaga gtattatgaa gatgcagcta tcatgttcaa taacttgatt
    13201 gtcaaaaact tgacagcagc tgttcttgcc ggtatcggta ttgcgttgct gaatctgaat
    13261 gtattagctt ctgctttgac atggcaattt gttgtcttat gtctgacaag tgttatcgcg
    13321 atttcagttg tttcaggatt tgtcggtcgt ttgtttggtt tatatccaat tgaatcaact
    13381 attactgctg gcttatgcaa caactcgatg ggcggaacag gaaatgtagc cgtcttatct
    13441 gcagctaatc ggatggaact tattgctttt gctcaaatgg gcaatcgact tggtggtgcc
    13501 atcgttttga tcatctctgg tttcttgatg caattacttt cttagtcaaa aaaacaaaaa
    13561 tacctgatca tcaagtatcg gcttggtgat cgggtatttt tttgataaag atttacagga
    13621 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgtgttca taaaattggt
    13681 gtgcttctac tctgtattct gcagaattaa gccttattgt cgttattcct cgttttttcg
    13741 cttcttcttc tacgccagtc atcaaagcct ttccgatgcc tcttttttca gcatatttag
    13801 acacagcaag tgccataata ttgaacattg gatcgctaaa aatcgattca taggactccg
    13861 cttgtacata gccagctgct tttttagaag taggatcttc ataaaccaag aaaaaatttt
    13921 tctctggctc cttcagcagt ttcttcattt gtctcaccgt aacggttaat ggtatatcat
    13981 accctaattg atccgcattg atttcacgta gtgcttctgc atcttctaaa cgcatttccc
    14041 gtaacatcca aatcaccagc caatttctga ttttttaaaa tgaatttttt tatcatagta
    14101 tactcttttt ttcatttaga acaatatgtt tcagtgattt tgacttgcgt tagtctagat
    14161 tctcctaatg tttgtaagat gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg
    14221 acaattactt gaaaaataaa ggtcactttt tttggagaca aaaccctgct aaatcttgct
    14281 ccccacaaac ctccaataac agcagcgggg ataacataaa acaacataga taaatcaaag
    14341 cgcataaagc tacttgttaa agcaatcgtt accaattttg ccagttgaga aaagaaaatc
    14401 gtagatagag aatatagtgt tgcttctttt attggcaatg caaacatcag catcaacaaa
    14461 gaaacattga ctggtccacc accaatacct aaaaaactag ctaaaaagcc cagaaccccc
    14521 ccacatataa ggtaccaagt ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg
    14581 tagaagaacg caaaaaccaa ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc
    14641 ttctcgtttt caaataactg taaaaaaact tcgaatacaa tgttgccgac tactcctcca
    14701 acaacagctc cactagatac ccaaccgaca atctgccaat tcagtgattt cccgcttgat
    14761 acttgtcgca cagtcgaaac aatggacatc gtgaataccg caactgttga gtaaaaagaa
    14821 atccctgcaa cagaatgggc gccgataaga tcaagaatcg gtttgatcaa cacgcctcca
    14881 cccatcccgg aaatagcgcc aactgtgttc gccagaacga tcaccacaaa ataaatcaga
    14941 ccaatcatct tgtatttcct ttctcttgtt tatatctttt agcgattgtt actcttttct
    15001 tttattatac agtaaaagaa acaaacgagg cttcgaaagc ttttctactt tttaaggaaa
    15061 tttagtgcta aacgaa
//
LOCUS       DAXWLG010000056        14730 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXWLG010000056 DAXWLG010000000
VERSION     DAXWLG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.056"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACGE4T_001388"
     CDS             complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACGE4T_001388"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFF8386673.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRGERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACGE4T_001389"
     CDS             complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACGE4T_001389"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8386674.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8110..9078
                     /locus_tag="ACGE4T_001390"
     CDS             8110..9078
                     /locus_tag="ACGE4T_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8386675.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9096..10001
                     /locus_tag="ACGE4T_001391"
     CDS             9096..10001
                     /locus_tag="ACGE4T_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386676.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9998..10804
                     /locus_tag="ACGE4T_001392"
     CDS             9998..10804
                     /locus_tag="ACGE4T_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386677.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10961..11929
                     /locus_tag="ACGE4T_001393"
     CDS             10961..11929
                     /locus_tag="ACGE4T_001393"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFF8386678.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12246..12770)
                     /locus_tag="ACGE4T_001394"
     CDS             complement(12246..12770)
                     /locus_tag="ACGE4T_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFF8386679.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13057..13689
                     /gene="scm"
                     /locus_tag="ACGE4T_001395"
                     /pseudo
     CDS             13057..13689
                     /gene="scm"
                     /locus_tag="ACGE4T_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
     gene            complement(13699..14658)
                     /locus_tag="ACGE4T_001396"
     CDS             complement(13699..14658)
                     /locus_tag="ACGE4T_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8386680.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         4154 a         3153 c         2886 g         4537 t
ORIGIN      
        1 aataatttgt cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct
       61 tctgcagcca tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg
      121 acttcttttg gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta
      181 cctataataa cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca
      241 tccgtcaaga cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct
      301 aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag
      361 actagcgtat tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag
      421 atatctgtat cacctggatc cattacacga actttacgca gcatttgacg aaccattact
      481 tcgatatgct tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga
      541 agcaagtagt tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt
      601 gatccttctg tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt
      661 gctgtataag gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg
      721 gtacgtgttg caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact
      781 gcttgccctt tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg
      841 atatcgtctc cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt
      901 tcaccgattg attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca
      961 gttgcaaggt tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag
     1021 acagaacgga ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg
     1081 gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta
     1141 cgggtgtaac gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg
     1201 atggcttgga tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct
     1261 tgggccacgt caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta
     1321 tcggtcatcc ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct
     1381 tcacggaagt tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa
     1441 ccacgcattc cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac
     1501 atcatgaaga ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct
     1561 tttgcagcat tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca
     1621 cgacggaatt gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt
     1681 ttttcattca gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt
     1741 ttgtacccta agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt
     1801 ttgaagactt ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct
     1861 tgctctttga tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt
     1921 tggacggtca agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc
     1981 tcgttgaaga tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta
     2041 aatggttttt cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat
     2101 ccgttgcgcc atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg
     2161 cgtccttctt cttccattgt caagtagtag ttacctagaa ccatatcttg agaaggagta
     2221 acaactggtt taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt
     2281 gcttctgctt gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa
     2341 tcggcattat aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa
     2401 actggttcga atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact
     2461 gggtgttcct tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt
     2521 ttacgtttcg catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg
     2581 aatggtttga ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag
     2641 aaaggtccaa caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt
     2701 tgacggaaac gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta
     2761 cctggtccag taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct
     2821 tgcagcatac gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt
     2881 ttcaaacggt tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca
     2941 aaacgtccac cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg
     3001 tccataacca tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa
     3061 cggcggattg cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg
     3121 gctgcttctg catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata
     3181 gcagcttgga attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac
     3241 aattgttttt tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa
     3301 atgatttctt ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc
     3361 cctttaaagt accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca
     3421 ccgcgaactt tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa
     3481 cggatacgtt tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc
     3541 tcacagaaca acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt
     3601 tttacttcac cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata
     3661 ctttcgaatt tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc
     3721 gcaagacggc tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct
     3781 tctgcggttt caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct
     3841 ttcttttcta attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg
     3901 atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc
     3961 aagacacgca tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc
     4021 ggtacacctg gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt
     4081 ccgactacgt catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa
     4141 gcttccagtg cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct
     4201 cccaatggtt gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca
     4261 tcgaccatgt gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat
     4321 ggttcacctg tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca
     4381 cgaactgttt cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg
     4441 atacctaact gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata
     4501 cgtgaaggca cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat
     4561 ggcatatctt cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc
     4621 attttatccc cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt
     4681 acgcctggtg acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata
     4741 ccgccgccgc catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca
     4801 aagatcgcat gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact
     4861 ttccctacaa gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg
     4921 tctaaatctt ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt
     4981 cctaattttg tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg
     5041 tcatctttta ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa
     5101 gtcatgaaac caactaaaac gttttggcct agagccattt cgccttgttc catagatggt
     5161 ccgtctgcca gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg
     5221 ttgtagcttg ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca
     5281 ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt
     5341 ttacataaca aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc
     5401 catggtgctt gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag
     5461 gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact
     5521 tgtttaggtg atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt
     5581 tgagcacgag ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt
     5641 gcttgggcta cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta
     5701 acacgacctg tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt
     5761 actttcgcat aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg
     5821 atcggacaca tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg
     5881 tcacgagtca atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca
     5941 agagggtttg tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata
     6001 cttgccacaa ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg
     6061 atcgacattc tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt
     6121 tgtaaaagtt caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca
     6181 ttgccgatac cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga
     6241 cgaaccgttt ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca
     6301 gggtctcttg gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg
     6361 tcttctgatg gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt
     6421 tccatgactt ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga
     6481 tcaactaatg tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg
     6541 tttactttgt aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca
     6601 ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa
     6661 acgtctttca aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg
     6721 actgtattgc gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca
     6781 aatcctaacg cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca
     6841 taagagatgt cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca
     6901 gtagaaccga atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt
     6961 gaacggacta actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt
     7021 tctgtcatta atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg
     7081 cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga
     7141 gcttctgcta ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga
     7201 ttgccattga agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct
     7261 aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca
     7321 ctgattcttg cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc
     7381 aagctcttca cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa
     7441 tatttcaaat attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa
     7501 aaccaaaaat agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt
     7561 atattaaagc cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg
     7621 caacttcata tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc
     7681 tttttcgcaa cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt
     7741 aattgacaga cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc
     7801 tatgcgaacc aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta
     7861 ctttagatag gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta
     7921 tgatggctaa agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct
     7981 gagcagtgaa aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga
     8041 cttttcaatc catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag
     8101 gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta
     8161 ggaggttgcg cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc
     8221 gtattgcaac ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga
     8281 ttagcagaag aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa
     8341 aacaatcaag acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg
     8401 ctacttggaa tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg
     8461 atgactgtca cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa
     8521 cctggcggca atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta
     8581 ctgttaaata tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca
     8641 aactctaaga tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc
     8701 aaagtactga cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa
     8761 gatgtagatg gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc
     8821 ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa
     8881 gacggcggtc tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc
     8941 atggcagcaa aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat
     9001 gatttggaac tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc
     9061 atcaaagaag ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct
     9121 tatcatctgc atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt
     9181 tccgtatctt ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga
     9241 tcgcggcagt aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt
     9301 tagggggtgc ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg
     9361 cccttcttgc agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg
     9421 gtgcgccgaa catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg
     9481 gcataacaaa agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat
     9541 gtttacttgt tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata
     9601 atctggcaat gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata
     9661 tgatttctaa tggatgcatt gctctttctg gctccttact tgcgcaaaac aacggctttg
     9721 ctgatttaaa ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag
     9781 aagttttgtt acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg
     9841 tcatttatcg ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga
     9901 aacttgcttc tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat
     9961 tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga
    10021 aaatctccat catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat
    10081 taatctttcc ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa
    10141 atctactttg ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt
    10201 gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt
    10261 attccaagat cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct
    10321 tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac
    10381 atttttcaaa gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga
    10441 aatcggtttg ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca
    10501 gcgtccagaa ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa
    10561 aacggtgatg gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt
    10621 cactcatgac atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg
    10681 aaggatcgtc gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt
    10741 agcgttgttc aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc
    10801 ataatcagaa agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc
    10861 ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc
    10921 cttcttcctg atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt
    10981 acttcaatta ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact
    11041 ggaagtttcc cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg
    11101 ttttgagcac gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga
    11161 aatccagcaa gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc
    11221 tgaatctacg atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt
    11281 atttgttgct gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga
    11341 acctggacga ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt
    11401 gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg
    11461 tcgttctgct tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg
    11521 gattttatct gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg
    11581 gatcgggctt aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc
    11641 catctctgtc tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat
    11701 ctggcaagaa ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga
    11761 aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg
    11821 tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat
    11881 gagtttgtct tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt
    11941 cacaaaagga cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc
    12001 tccttattct atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc
    12061 tataaagcat ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt
    12121 ttacacgatc aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg
    12181 gtaacgataa aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta
    12241 gtgccttaat ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata
    12301 gcagcatcat caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc
    12361 tcgtcaatta cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa
    12421 tcagcagctc ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt
    12481 gcttgttcca gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg
    12541 ctttcccagt cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa
    12601 tctaattctc gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta
    12661 tcttccactg gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac
    12721 cgatcgtatg cccgatccac actctcgcat agcatatcta aataatccat ttattttcct
    12781 cctttttatt tatcctactt actttttcct aattctagga tacctactta tatgttacta
    12841 cacaccctta ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga
    12901 aaaagatagt gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata
    12961 ttctttaaat aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat
    13021 aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta
    13081 gtcctagttg ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca
    13141 tacattccta aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta
    13201 ggaattgcta gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca
    13261 gacggaaata tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat
    13321 gaaggtttat tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct
    13381 tcaggcatac ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat
    13441 gtagcacttc aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca
    13501 gatgattttt ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct
    13561 aaattgaatg tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct
    13621 gcagattttc cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg
    13681 tttttattgt cttaattgtc aaattaagtt agacagatca tcaatactta cgtaactcaa
    13741 aaatacttcc aaaggtgttc gataatccag tgattttcta ggaatattat ttcgtttaga
    13801 tgcgacagat tgaataaaaa attcatctac tgaattgaaa tccatagatt tcaataaacc
    13861 atctctacgt aacaatccgt tagaattctc gtttaggcct ctttgagacg gcgttcctgg
    13921 atcagcgaaa taaatggcaa tatcattgac attactgatc tgtttccaat ttgaaaattc
    13981 ctttccacaa tcaaaagtga tggatttgaa taggtttttc ggtacagatt caaaccattg
    14041 atttaatttt ttttcaatat caatcgcttg tctaccacac ggtttcaatg tgatgataac
    14101 ttttgataat cgttcaacta aggtaattac agcactttta tgtttcagac ctacgatagt
    14161 gtcaccttca aggtgaccaa actcatttga gaattggcta taatcctttt cacgttcatg
    14221 aatagagcgg cggaaagttt gttttccacg tctttcttga tgtccattcg gtttacgctt
    14281 gcctttcatc ggtaagtcag aagaatcaaa tagccccttt ttgaacatac gataaatggt
    14341 acgagcagaa caactaatag gaaacgctgc acgaccaaca atcacatcgg gtgtccatcc
    14401 ttgaacaacc tttctttgaa tatattctga ttgttcctcg ggtaaaacaa gcggacgtct
    14461 accacagttt gatttgtttt tcttatactg ttgataatat tctaaggctg attttccttg
    14521 cttgaagaac agatatactt tatggatcgt ttgtcttgaa cgattcaagc aatgggcagt
    14581 tttagcaaca gattgattca ttttgaaata agactctatt attacaagtt catccgttgt
    14641 aagatgggta taggtcattt gtactcactc cttataattc tttggtcgga actattttga
    14701 gtgtagcaca aatgactttt ttagttgtct
//
LOCUS       DAXWLG010000057        14726 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXWLG010000057 DAXWLG010000000
VERSION     DAXWLG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.057"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGE4T_001397"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGE4T_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFF8386681.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACGE4T_001398"
     CDS             complement(481..675)
                     /locus_tag="ACGE4T_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386682.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(901..2103)
                     /locus_tag="ACGE4T_001399"
     CDS             complement(901..2103)
                     /locus_tag="ACGE4T_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8386683.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2106..2300)
                     /locus_tag="ACGE4T_001400"
     CDS             complement(2106..2300)
                     /locus_tag="ACGE4T_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFF8386684.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(2347..3195)
                     /locus_tag="ACGE4T_001401"
     CDS             complement(2347..3195)
                     /locus_tag="ACGE4T_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFF8386685.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(3400..3750)
                     /locus_tag="ACGE4T_001402"
     CDS             complement(3400..3750)
                     /locus_tag="ACGE4T_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386686.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3928..4791
                     /locus_tag="ACGE4T_001403"
     CDS             3928..4791
                     /locus_tag="ACGE4T_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8386687.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            5415..6407
                     /locus_tag="ACGE4T_001404"
     CDS             5415..6407
                     /locus_tag="ACGE4T_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386688.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            6423..7307
                     /locus_tag="ACGE4T_001405"
     CDS             6423..7307
                     /locus_tag="ACGE4T_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386689.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            7356..8963
                     /locus_tag="ACGE4T_001406"
     CDS             7356..8963
                     /locus_tag="ACGE4T_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8386690.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8964..10448
                     /locus_tag="ACGE4T_001407"
     CDS             8964..10448
                     /locus_tag="ACGE4T_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFF8386691.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            10625..11617
                     /locus_tag="ACGE4T_001408"
     CDS             10625..11617
                     /locus_tag="ACGE4T_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8386692.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            11669..12538
                     /locus_tag="ACGE4T_001409"
     CDS             11669..12538
                     /locus_tag="ACGE4T_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8386693.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12958..14571)
                     /gene="guaA"
                     /locus_tag="ACGE4T_001410"
     CDS             complement(12958..14571)
                     /gene="guaA"
                     /locus_tag="ACGE4T_001410"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFF8386694.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
     gene            complement(14578..>14726)
                     /locus_tag="ACGE4T_001411"
     CDS             complement(14578..>14726)
                     /locus_tag="ACGE4T_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8386695.1"
                     /translation="AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGL
                     IFEN"
BASE COUNT         4502 a         2531 c         3014 g         4679 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg
      781 cattccccat ccatattata tttacgtctt attcccactc aatatgagta gcctcgtaaa
      841 gaagctatta tatcagtgct ttttcagcct attcctcatt ttggtaggga aatggtaggg
      901 ttagattttt actgcttgat ttccattaaa gtttgcaact ttagattttg ttgcacaggt
      961 aaatttaatg tttcctgtca tttgttctgc aattccttca tcattacggc tccatagatg
     1021 agcgtagtgc tttaaagtga tttcagggct agaatggcct aaccgttgcg acacgaccag
     1081 aatgtctgcg ttaaactcat tgattaggta gctaacatga gaatgacgta atccttttcc
     1141 ttgtattagg ggtaccttag ctagtttggc ataacgctga ataattcgtt gaacagttga
     1201 acgatagagt gggaggtcag tgtagctaag aataaattgt tctacaccat gatttttttg
     1261 aataactttc cactttttta gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa
     1321 gccgctttca gtttttgtgt aaggtttccg aataaaatct ttttgatttg tcatttccaa
     1381 ggtgtgatga accctcaact ttttacgttt gaagtccacg tcattccaat taagtgctaa
     1441 tccttcgctt acacgaacac cagtcatgta atatagccag atcattaaaa aacacatatg
     1501 ttcgtagaaa tcatctttat aaatttttgc caaaacttct tcaaattctt cttttgtcca
     1561 gtaaggaacg attgattttc cttttggaat tgcttttgtt cttttggaaa tattctcatt
     1621 cagaaattgc agggtgaccg catagtctaa tgatttacga aacataccgt acataagtga
     1681 actgtatgct tgtgagtatc cgctatcgtt caataaccaa attcgataat tttcacaatc
     1741 tcgcacattg atttcacgaa gcttcttatc accgaaaaat tcagtaatct gaatcaatcc
     1801 acactcacga gaactccaag tacttttttg tacgctagct ttgtagtgtg gaacgtaaga
     1861 ctgctccata aattctttgt aagttaaatc ataattcgca aatccattta caaccatgta
     1921 ttcatttttt atacgcatga cttctttgtg tgcttcacga gctgatgaga atcgttttcc
     1981 tagagaatct tttgttcctt tcttctggat cctctttcca gtcacttgat ctgttcctaa
     2041 ctcaactaga taagaaaagc gaccattttt atcttgatac acacaagggt attttgtttt
     2101 catagttatt cctcctgtga aagaggaaca cctataattt cttcaattac ttgtcggggt
     2161 actaccccaa ctcttttatt attataatat ggatatccct gatttaccat tcttgttttt
     2221 gcttgtcgga ttattgtggt agcttgatgt gttttaaatc ctagagcctc taaatctttt
     2281 cgacaaattg tatttttcat tgtataaact cactttcaaa gaaaacacta tcattgggta
     2341 atagtgttat ttctttattt ttgtataatg aacggtactt tcttgaaaat ccatcacttg
     2401 ttcaggactt cccaactcat ttaaagctaa agcattgtaa aaaaatggac gtctaactat
     2461 atattcgctt gtatatagta tatcttgatt cgtggaatca tagggtttct ttaccatctt
     2521 tttggtgaca gttggtagtt tgagattccg ggacttgaaa aaagcttttt tcttatgctc
     2581 tttcaattcc agctctttat cgaaatattt gctgatatag cgcccgcgat tttcgatact
     2641 gtctacatcg atcttgttga tcttaacgaa accctgaccc caaatcgtag ttaaccggtt
     2701 aaatgagata aaggggaaat caaacaagat aatatggtag tggattgcac cgcgttgctg
     2761 cttttcccac gtggcgagat actttaaatt tgctttttta gttttataga ggtcataatt
     2821 cagtcgtttg ataaattttt taaattcatt atttgcatac tcaatgtctt gaatgttttc
     2881 tttgaaagtg agagttaaaa atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc
     2941 aatctcaaat cgcatacttt gataatgttt ctgtttgcgt tttaaactgt catattgtcc
     3001 ttgagcggat aattcatcat agttttttct tttttcaaga gtaatatcat tttcataaat
     3061 ccaagctaat ttttgttttc gttccagatt tacttcgttt tcgatcgttt tacgtttggg
     3121 agtaatgatt gcttcataat cataaatttc aatatacgtg ggagtttcaa tgattttctg
     3181 attataagct tgcaaaaatc atcactcctt gttgcgtatt cgattgtcaa agaaccagtc
     3241 attttctcct gatgttgatc tataaatcaa gttaaggggg aaggcctgcg tagcagccct
     3301 tctagcctat ggggtgttgg gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc
     3361 cgccgtttcg tcctaaccac ccaacactcc atagtatagt tagttttgtg tagtggtacc
     3421 ttgttttctg ggaatcaaag ttgccgtatc agcggttgcc gataacccat aactgacaaa
     3481 ttgtccattc gagcgtcccc aaggtacaat caccaaattg gtaaatgtaa ttatggctaa
     3541 accttgttgg gtaagttctt ccggcgtgat cacaggatgt tcagatttga ctttgacact
     3601 gattttttcg tagcgaagtt ttggtaggat acattcgtag ctccaaccta gaatttcgcc
     3661 ctccttttgc cagcgagtga cattgacgac ttggtattct ttttcgagtg tttcttttgg
     3721 taaactgaga tcggtaattt ttattggcat cacaattctc cttttttata cgaaagttta
     3781 tacgatttga ttaaaaaata tagatggcca tctacgactt gtattatata cgagaactta
     3841 tacgtagtca agagattttc taaaaaaata tatacgattt tttatacgag tgttataatg
     3901 aaaaaaatga taaggaggaa tttgaatatg tttggtttag tacaacctaa taaagtgaag
     3961 gtgggccaac gaatcaaaga aataaaagac ggaatgtctc tctcttttac agaattaggg
     4021 aatcgattag gtttaaaaaa gccgacgatc agttcctacg ttcaaggcta tgcgttggct
     4081 cctaagagtg tgatcaacca attatctagt atcagcggaa aacctgtcgg ctggttttat
     4141 tttgggcatg tggaagaata tatccgagag tatttgtttc tcattggtca gaaaaaaata
     4201 gtggacgatt atcctgaaat cattgagaaa ataaagcaag aattttatac gggcgagttc
     4261 aaaaatcctg cttgggagaa tgagctaggc tatcctttgg aggaatttat ccaagaatgt
     4321 tttgtagaat attcgagtga aatcatgaag gactatgtcg tacagattac gaaagaggta
     4381 cttgaagaat ccaaaacgct aaacaaacta tcatcaaaag caaaagaaga agctgtcacg
     4441 attctttcat ctgggatcat tgactatatc gagatgagtg gtgagatcaa gtatggagag
     4501 aaagatcgca ttgcgaaaat ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac
     4561 ctgacattta atgattccta tttgattgga aagttgatca atattcttgg tacagatgaa
     4621 gatacggagc tgtttctttc ttctttatca atgatattta ctgataagca tttttccact
     4681 cgatttggtg gggaagaatt agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa
     4741 ctctatcggg agaaaacacc agatgaattt tatgattggt ttgagaagta aaataagaaa
     4801 agtggcttag attacaagtt ggtgaaaaaa tttttttcag cataacatca gtaatctata
     4861 aaggggttgt tgattatact gtagaagtta agtactcaag agattttttg tacgtaaaca
     4921 aagcaaaaaa gggtgcagtt tcactagaaa atgtactctt ttttgtgatt tttcttagtt
     4981 atagagtttt tttgaaactt taaaaatgag aaaacatatt gattttaaag agtgtagaca
     5041 tagcttcttt tgctaaatag attaaactta ctggtgagtg gtcttgaaca tacttttaaa
     5101 tcacatttta ctgagtttta ttggatttta taattcattg gaaacctcgt acttaatctg
     5161 tttttatcta tcgactagaa tctgtatata ttccagctat caatgaggcc gcagaaaagg
     5221 gtgcttaaaa ataatctgtt gtttaaaata ttattatcag aatacttaga tacaaaattc
     5281 tggcacaaag taaaaaaaat attgacttaa aaaaatggat atggtaatct ataaacgaaa
     5341 atagtggtaa cgtttccaaa atgcatcttc gttaaatttt tattttttta aacgaaaatg
     5401 gaaacgtttc taaaatgtat tgggaggaga ttgaattgga aaagacattg ggaccaggtg
     5461 gtgagataag ggcggtacaa cgggataaaa aaaatatttc gaagtgggaa aaattaaaga
     5521 aacagttgcg gcgtgataag tggatgtact tgttgctctt accaggtatc atcatgacgt
     5581 ttatctttaa gtatgtgcca atgtacggtg ctgtgattgc atttaaagac tataatccta
     5641 tgctaggaat tttggaaagt ccttgggtag gactagatca ctttagggaa tttatcagtt
     5701 caccaaattt tacgcttttg ctgagcaaca cattaaaaat tagtatttat ggtttattgt
     5761 taggtttttt tccaccaatt attttagcac tgagcttgaa tcaaattatc aataaaaaaa
     5821 taaaacagaa aatccagttg attctatatg cgccgaattt tatctcagta gttattattg
     5881 tggggatgct gtttttgttc ctatccgcaa atggacctat taatgggtta gtagaaaagg
     5941 ttacggggca accaattatg ttcatgtcta atccagctta tttcagaacg atttatattt
     6001 tatcgggaat ttggcaagga atcggctggt catccattct ttacaccgca acgctggcgg
     6061 gagtcagtac cgatttatat gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga
     6121 tttggcatat cgatttgcct gcattgaggc ctgtgatggt gatcagcttc atcttggccg
     6181 ccggcggtat tatgaatgta ggatatgaaa aagcctactt gatgcaaact tcaatgaaca
     6241 tccctgcctc agaaatcatt tccacttatg tatacaaagt cggcttgcaa tcaggtgatt
     6301 atgcctactc tgctgctgtt ggtctgttca atatgattat taatgtcgtt ttattactga
     6361 ttgtcaacaa tataaccaag cggctaaacg atggtcaagg cttgtaatga aaggagaaaa
     6421 ttatgaatag tgctttatca aaaatcgacc gtcaaatctt atggttgaat cgagtgattg
     6481 taactttttt agtcgtcatc accattgttc cgctgattta tgtactggta gcttctttta
     6541 tggacccagc tactttgatc aataaaggta taagcttcaa tccgaaagat tggacaatac
     6601 agggatatca acgggtattt gcagatgatt ccattttacg cggttttgta aattcgttgt
     6661 tttactcttt tagcttcagc ttattgactg tatttattac gatgatcact gcgtatcctt
     6721 tgtctcgacc taacttggcg gggaaaaaga ttattatgac tttttttgtc attacaatgt
     6781 tcgtgggtgg tggcttgata cctacctatt tattggtaaa gaacttgggc atgttagata
     6841 gtgtatgggc gattatcgta ccaggtgcta taaatgtgtg gaatattatc ttagcccgta
     6901 cctattttag tgctttgcca aaagaacttt ctgaagctgc tactatcgat ggcgccaatg
     6961 acttgcaaat actgttcaaa gtcatgcttc cattagcgaa accaattatg ttcgtattgt
     7021 ttttatatgc ctttgttggg caatggaact catactttga tgccatgatt tacatcaaag
     7081 atcccgactt acagccgttg caactcgtat taagaaatat tttgatccaa aaccaacctt
     7141 cacaagacat ggtgggagcc aatactgcca tggcagaaat gaaacaaatt gctgagttga
     7201 tcaaatacgc aacgatcgtc atttctagtc tgccattaat cataatgtat cctttcttcc
     7261 aaaaatattt tgataaaggt gttttagttg gttctgttaa agggtaagta ttgttcacta
     7321 tttgtatgaa taaaaattat aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa
     7381 tcgtttttac ttccgtacta attttggggg cttgtggagg ctcaaaagaa aagagtaaat
     7441 caagccctga ttatgaacta gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa
     7501 aaatgatgtc tcaaagtgcc cctttggctc ccaatgatcc taatgacaaa ttgattttcc
     7561 aacgattaga agaagagacc aatctccata ttgattggac aaactacagt agtgattttg
     7621 ccgaaaaaag aaatttggat atcgctagtg gtgatttacc agatgccatc ttcaacgccg
     7681 ctgcaggtga ctatgattta ttaaactggg cggaaagtgg cgttattgta ccgattgaag
     7741 atttgatcga agattatatg ccaaatctga agaaaatttt tgatgaaaac cccgaatacc
     7801 gacaaatgtc tactgcacct gatggtcaca tttactctat gccttggatt gaagaattag
     7861 gcgaaggaaa agagtcgatt catacagtaa atgggatggc ttggatcaac aaggagtggt
     7921 tggataattt gggtcttgaa atgccgcaaa ccactgatca gttgatgact gtgttggagg
     7981 ctttcaagac gcaagatcca aatggtaatg gtgaagcaga tgaaattcct tttagcttta
     8041 ttaacaatgg aggaaacgaa gacttaaaat ttttaatggg ggcattcggt ctaggtgata
     8101 atgatgacca cttagtcgtt gataacgatg gtcaggtaca atttactgct aacaaagaag
     8161 ggtataaaga agccatcaaa tatttcaatg aaatgaataa taaaggtctt attgataaag
     8221 aggctttcga acaagattgg aatacgtatg ttgccaaagg gaaagaacat aagtatggcg
     8281 tgtactttac ttgggataaa gcaaatatca cgggtgcaga tgatagttat gacgtcttgc
     8341 cagtcttagc gggaccagat ggaacgaaac atgttactcg tacgaatggc atgggtttct
     8401 caagagatcg tttcgtaatt actagtgcga ataaaaactt ggaacttaca gctaggtgga
     8461 tagataaaat gtatgaaccg ttacagtctg tccaaaataa ctggggaacc tacggtgatg
     8521 aagcacaggc gaatattttt gaattaaagg atggtatgtt aaaacatttg cctttagagg
     8581 ggacagcacc gggggaacta agacaaaaaa ccgaagctgg aggaccatta gcagttcttg
     8641 attcttatta cggcacagtc actactatgc cagatgatgc gaagtggcgt ttagatttga
     8701 tgcatgacac gtatttgcca tatattacag aagacaatat ctatccacgt gtctttttag
     8761 aaaaagaaga aacagatcga ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc
     8821 gcaaaagagc agagtggatc accggcggta aaatcgatga agaatgggaa gaataccttg
     8881 ctgaattgga acgtgtaggt ttggaagaat ggattcaaat aaaacaaact ggctatgata
     8941 attttttgaa gggagcacaa taagtgttag tagaagaaac ggcgattttg gataaagcaa
     9001 atcgatttat tgaaatgatg cgaggaaaag ttaaccctca gtatcgacaa aactatcatt
     9061 tttccgcacc catcgggtgg ataaatgacc ccaatgggtt tatatattac aaagggcatt
     9121 accatctctt ctatcaatac tatccttacg atagtcgatg ggggcccatg cattgggggc
     9181 atgcgcgcag tagtgatttg gtccattggg aagatctacc agtagcgtta gccccagatc
     9241 aaccttatga cagcggcggg tgtttctcag gaagtgcaat tgaaagagac ggaaaactat
     9301 atttatttta tacaggctgt caagaaattg atggtattag ccatcagcat caatgtttgg
     9361 cggtctctga agatggtatt cattttagca aatacagtgg taatcctgtg gtagcagaaa
     9421 aacagctagg tgaaaatggg ctaatacaag aatttcgtga tcctaaagta attgttcaca
     9481 acgatggtta ctatatggta gtagctacta aatctacaca gtctactggt agaatagtga
     9541 tgtttcaatc gtcagatttg tatacttggc acttttactc tgtgctgcta gaagggactt
     9601 cagagcaagg aataatgtgg gaatgtcctg atttattcca tttagacgga cgagatgtct
     9661 taatcatgtc tccgatacaa atcaagaaga acggactgga atatcataat atcagttcta
     9721 ccatggcttg tattggtgag gtaaactggg aaacaggtca gatgatagtt gataatttcc
     9781 atgaaatgga ttttggaatg gatttttatg caccacaaac aacaagagat cctaatggtg
     9841 gacgggtgat gattgcttgg atgcagatgt gggatcgtac attgcctaca catgagttag
     9901 gccatctttg ggcaggctct atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat
     9961 tggtacaaaa acccatctca actgtatata gtcatttagt ttatcaacaa ggcagtgaaa
    10021 atattgcagt gactgacaca tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt
    10081 tacatctagt cgcagatttg gagaaagctg aagtttttac aattgaatta ctgcggtcta
    10141 agacggattg tctcaaacta gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc
    10201 agaaactagg aagagagctt acaggggcgg aagaagaccc tctgaatgta cgaaaagttg
    10261 aagtaccttt gattgataat cagctcgttc ttgagatttt tagagatacc tcatcaattg
    10321 aagtctttat taatgggtta gaagttatga cggctacttt ctatgagctg gaaaaagctc
    10381 gagatatctt gtttcattcg atcggagaaa gtcgtattca agcttttgaa acagcgagtg
    10441 tgaaataaat gtttgattgc ttaggcgatg aatcaattat ttaataggtt ttgaaggata
    10501 atgtttttag aaaagaacag atagctagat ttcccatgga tttgctatta gtattttgaa
    10561 aaaattagta atttatgaca aaattcatta tactaaactt gtgcagaaat gtgagggatc
    10621 aactatgaaa gcaacaatga aagatgtcgc aaaattagca ggtgttggag taggaactgt
    10681 ttcccgggta ttgaataacg gttctgttaa agcttctact agagaaaaag ttgaagcagc
    10741 aatgacagag ttaaactatc aaccagattt ttatgcccgg gggttaaaga tgaagcatac
    10801 gaacacggtt gctttgattc tacccacggt atggcatcca ttttttggcg aatttgccta
    10861 ccatgtagaa acagcattag aaagacaaaa ctacaagctt tatttgtgta actccagtgg
    10921 taatagtgaa aaagaacaag aatatatcca gatggtcagc caaaataaag tagacggcat
    10981 cattggaatt acttattcag atatcgatga atacatttcg agtaatctgc cttttgttag
    11041 tattgaccgt tatttcactg aagaggtgat ttatgtgacc gctgacaatg cggccggtgg
    11101 acgtttggct gcaaatgaac tttatcgccg aggatgtcag aatgtagcat atatcggtgg
    11161 gtaccaagaa acaccgaatg aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg
    11221 tcaactgggg atttcctata aagcgttaga tatgttagaa cctttagacc atctctcaga
    11281 acgtgtaaca aattttttgt tagagaatcc cgatatcgat gggattttta cgatcaacga
    11341 tccaatggca ttagctgtca ttgatatgct tgaaaaaata gggaaaaacg taccagagga
    11401 tgtccaagtt atcggatttg atggtcaaaa gatgacagaa ggagatcctt atctagtctc
    11461 aacgattgcc caacctacaa agcaaatggc ggaagtggca gtggcaaaat taattgctgt
    11521 cattaatgga gagacagtag aaaaacgtac cgtattgccg attgaatttg gcgaaggtga
    11581 cacaacgaaa cctttagtga gaaacgattt tgcttagtgc aaatacgttt ctttttttga
    11641 aaatgagtga atgaaaggtg tagataaaat gttatatggt ggaatagaag ccggtggaac
    11701 aaagtttgtc tgtgggattg gtaatgaaca attacaagtg atagaacgga aaagttttcc
    11761 taccacaaca ccagaagaaa cgatggagca agtgtttgat ttctttgata agtacaaagg
    11821 aaatatcgca tcaattggtg taggagcatt tgggcctatt gatattcaaa tcgaatcaag
    11881 aacttacgga tatatcacca acacaccgaa gttagcttgg caaaattatc attttgtagg
    11941 agctttggaa aacgccttgt cggttcccat ctattggact accgatgtaa atgctgcttg
    12001 ttatggagaa tatatggcag ggtatggaaa aggtaaaaat agtgttctgt attacaccgt
    12061 tggtacaggt gttggtggtg gtgctgtagt ggatgggaaa atagtcgcag gttttagtca
    12121 tcctgaaatg ggacatatgc tcttacaacg tcataatgaa gatcaatttg aaggaatctg
    12181 tccttttcat catgattgtt tggaagggtt agcatcagga cctgcgattg aaaaacgctt
    12241 tggaagaaaa gcccagacta tctcatttga tcatccttat tggacgattg aggcagatta
    12301 tcttgctcag tgtgtgtata acacaacgtt actatatgca ccggaagtaa taattttagg
    12361 cgggggtgta atgaaacagc cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa
    12421 aatgggttcc tacgtggata cgcctactct cgaagaatat ctactaactc caaggcttgc
    12481 tgacaatgcg ggcttgattg gctgcctagc tttagcaata aagaaactca atcattagaa
    12541 tagtatttat tagaactatt cataacatac cggaagttta aaagggtatt ctgaaactta
    12601 aatagtctca attcattctt ctttcttgag aatctgagta gagactttta ttctgtttca
    12661 aattaatata attacgaaat gatattgagt acaagaaagt tctttttacc agatctttta
    12721 aatcgaaaaa tctgatatat atatttatag tactaaaaat ctgattagat aaaaagctaa
    12781 ttcaatagga aaataaaggt agtgaataaa agcaaagccc ttgttagtag ttaatctaac
    12841 aagggctttg gtagggaaaa tggtaggaaa ccatcttatt ttttacgaaa atcaggaact
    12901 ttagtaattg aataatgttg atttataggt attcctcatc catattatgt tggctcttta
    12961 ttcccactcc actgttgcag gtggtttgct ggtaatatca tacacgatac ggttaacgtg
    13021 tgcgacttca tttacgatac ggacactgat tttttgcaag acttcccaag ggatacgggc
    13081 aaagtcagcg gtcatgccgt caatagaagt gacagcacgg atacctacag tgtagtcata
    13141 cgtacggcca tctcccataa ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg
    13201 ccagatgtcg cgatctaaac cggcattggc gatttcttcg cgcaagatag cgtctgattc
    13261 acggacgatt tctaattttt cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg
    13321 tccagggaat ggttggcgcc aaacgatgct gtcaggcatg cctaattggg tacctaattc
    13381 acgtacttcg tctttgaaca atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc
    13441 aggtaatcca cctacattgt gatgtgattt gatggtttgg gcagtttcag ttcctgattc
    13501 gatcacatcg gtataaaggg ttccttgtgc aaggaaagaa acgccttctt caccggcaag
    13561 tttggttgct tcatcatcga agacatatac aaattcattt ccaatgattt tacgtttttc
    13621 ttctggatca gacacgccag caagttttga taagaaacgt tctttggcat cgacttttat
    13681 aatgttcaag ccaaattttc cgccaagact ttccataact tgttctgctt cacctttacg
    13741 caacaatacg tgatcaacga agatacatgt taattgatcg ccgatcgctt tttgtaaaag
    13801 aacaccaaca acagaggaat ctactccgcc tgaaaggcct aataaaactt ttttgtcacc
    13861 gacttgttca cgaatttttg cgatctccat gtcgatgaag ttatccattg tccagtcacc
    13921 agtacattgg cagacatcta gtgtaaaatg gcgcaacaag tcatttccat attcggaatg
    13981 gcgaacttct gcatggaatt ggattccgta cattttgcgt tctgcatttt ggatcgatgc
    14041 gatggggcaa tctttgctgg tagcaacgac atcgaaatca gtcggtgctt gggtaactag
    14101 gtcaccatga ctcatccaaa cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc
    14161 tgcagaagtg atttccaatt ctgcttttcc gtattcacgg tttttggctg gtttaacttt
    14221 tccgcctaat ttataagtaa tcaactgcat accgtaacag attcctaaaa ctgggatccc
    14281 caaatcaaaa atcttttcat caataccaaa ggccttctca tcataaacac tgtttggtcc
    14341 gccagaaagg atgattcctt tagggttcat tgcctttact tcttctgcgg taatgcggtg
    14401 gctcatcaac tctgagaaaa caccgaactc acgaatacgg cgagtgatca gttggttata
    14461 ctgactaccg taatcaagta cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga
    14521 caaatttgaa taaaacttag tccgatatgc ggcgcgttct gtgcgccgca tattttttta
    14581 gttttcaaag atcagacctt gttgtaaata gatccgtgcc cgaaagttat aaaagttgcg
    14641 ataaccaaac gcaattcgtt taatcagctt gatcttgtta ttcagcccct cagtgacgcc
    14701 gtttgagtgg gacaccttaa aggcat
//
LOCUS       DAXWLG010000058        14541 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXWLG010000058 DAXWLG010000000
VERSION     DAXWLG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.058"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..606
                     /locus_tag="ACGE4T_001412"
     CDS             <1..606
                     /locus_tag="ACGE4T_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFF8386696.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
     gene            complement(772..1791)
                     /locus_tag="ACGE4T_001413"
     CDS             complement(772..1791)
                     /locus_tag="ACGE4T_001413"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8386697.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2424..3428)
                     /locus_tag="ACGE4T_001414"
     CDS             complement(2424..3428)
                     /locus_tag="ACGE4T_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8386698.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3441..4490)
                     /locus_tag="ACGE4T_001415"
     CDS             complement(3441..4490)
                     /locus_tag="ACGE4T_001415"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8386699.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4507..5328)
                     /locus_tag="ACGE4T_001416"
     CDS             complement(4507..5328)
                     /locus_tag="ACGE4T_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8386700.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5315..6094)
                     /locus_tag="ACGE4T_001417"
     CDS             complement(5315..6094)
                     /locus_tag="ACGE4T_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8386701.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6111..6581)
                     /locus_tag="ACGE4T_001418"
     CDS             complement(6111..6581)
                     /locus_tag="ACGE4T_001418"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8386702.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(6593..7012)
                     /locus_tag="ACGE4T_001419"
     CDS             complement(6593..7012)
                     /locus_tag="ACGE4T_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8386703.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7093..9870)
                     /locus_tag="ACGE4T_001420"
     CDS             complement(7093..9870)
                     /locus_tag="ACGE4T_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8386704.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            10061..10285
                     /locus_tag="ACGE4T_001421"
     CDS             10061..10285
                     /locus_tag="ACGE4T_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386705.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11064..12779
                     /locus_tag="ACGE4T_001422"
     CDS             11064..12779
                     /locus_tag="ACGE4T_001422"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386706.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12782..>14541
                     /locus_tag="ACGE4T_001423"
     CDS             12782..>14541
                     /locus_tag="ACGE4T_001423"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8386707.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTA"
BASE COUNT         4329 a         2970 c         2521 g         4721 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag
      601 aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga
      661 tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca
      721 tcttgactta tcaccattag actaaacgac gattaactat tgatcatcta tttaacgaag
      781 agaaaattca ttctccattt ttccgtcacg taacatgatt tcttttgctg cttttttgat
      841 atcttctcca ttatacaaaa cattatagat tgtttgagta attggcatct caacacctag
      901 ctgttgagct agctctactg ctgcttttgt cgttgccaca ccctcaacga ccattcccat
      961 gttttctaga acttcttcca gcttatgtcc ctgtcctaac aagttaccgg cacgccaatt
     1021 gcgagaatga acacttgtac aagtaacgat caagtccccc acaccactca aaccgataaa
     1081 tgtcagagga ttggcaccca tcgcgacacc aagacggctg atttctgcca atcctcgagt
     1141 catgattgct gcttttgcat catctccgaa tcctaatcca tggatagcgc cggcaccaat
     1201 tgcaatgata tttttcagtg ctgcaccagt ttctacgcca atcacatcat tatttgtata
     1261 aatcctgaaa taatcattca tgaacagttt ttgtacatag gctgctgctt tttgatcaat
     1321 acttgcagct gtaattgttg tgatatcatg tactgccact tcctcagcat ggctaggacc
     1381 agataataca acaattgcct gtcttttttc ttctggaatt tcttcagcca aaacttctga
     1441 gattcgttta tgcgtatctt gttctagtcc tttacttgca tgtataatga ttggcttagt
     1501 attcatttta ccaataagtt cctgtgcgac tgaacgaatc gcttttgttg gaacaacaaa
     1561 taaaatagca tctgcgttat ccactgcttc tgccaattct ttatatccga taatcgattc
     1621 tgggatcgtc aaatcaggta aaaaatgctg atttgtatga tacttattga tttcatcaat
     1681 ctgttcaggt acatttcccc aaatacggac atcatgcccg ttttctgcca atgtttgtgc
     1741 taaagcagtg ccccaagatc caggaccaag aactgcgatt ttttgtttca tggatattcc
     1801 tccaaataaa taattgataa tttccattat agtatacatt tcttaaagtt gggactaatt
     1861 tccattgttt ttaagtgttt gtttacttct tttatcgttt ttacttgaaa taatcaatga
     1921 tatgttacaa tagtctacat tttattttta cactcgttat gctttgcttg ttcctaagag
     1981 gaagcagttt tgacaatgtt actaccagca ttactattag gaggttagga aacttctcct
     2041 agtgagtatt gatttaaaat acataagaga cagattgaac atttctaaat ctgtcaggtc
     2101 ctgcaacgga cagattgtct ctgttgacgg ttgacatagg aacttttgtt ttagtcaaca
     2161 ttctggatac aaaacgctat gtaaactggc gcaactcgtt gtacttggac ttttactacg
     2221 gatccatgca acaactaaat agtactattg cataacaaaa aatggttggc acccacatgt
     2281 cccattaaac agattataag ttgaactgta gataatgcaa aaaatgtgtt ttataacgta
     2341 attgatgcca atattttaaa aaaataaagg gagcagttat cgctgctctc tttatttttt
     2401 atattctttt tatccagtct atcctaataa tcaaactgac gatagtagcg gcgtatctct
     2461 aatggatggc agttcaactg ctccacatga gcatcgatgc gttgtgtcac tacatgcatc
     2521 aataagaaag acaaaacacg tccgcgatat ttttcactga taccttctac tggatagtct
     2581 tttgtgtcta tcaatgtata attgctgcaa atttttggca aaagtttagc cacacgttca
     2641 gataacactc tctgttcatc ttcgcctaaa aataatgtga ctggggttgt ttcttctacg
     2701 atctctaacg tcccgtgaag aaactcggca gcatggatag atttcgatgg aagccagctt
     2761 tgttcttccc agtaacacat ggcatatgaa taaacagctc cccattgatt ccctgctcca
     2821 ataaaataat gcatactatc acgtcgatgt ttttctgcaa atgataagcc aaatgcatct
     2881 gcttttttct ctgcttccac taaccctgtt ggtaaataac gttcgagctg ttcatagtat
     2941 tctgaataag cttcgaattc tccattcaaa tacatcaatc gatctgctgc cataaaaaat
     3001 ttgatttgtt ctgttccagg agctggataa gtgaccacaa aatcgcatag ctgagacaac
     3061 ttactgttcg ctttatcgat aaatccaatc agtgtcgctc ctactttctt gatttgttct
     3121 actgctttca ctacttcttg agttgtaccg gtaacagagg acaaaataac gatagaatct
     3181 tttgttaatc ttttgtttcc agtagtgtaa tattctgcag catgctgtac aaagacaggc
     3241 aagttgcttt tcccattcat ataagtgaca gcttgcatcg aggaagcata cgttccaccg
     3301 attcctagat aatagacagc gtcaaatttt tccgcataaa gtttatcgat gatttcttct
     3361 acttgcggac gtaaagctag tgcgcctttt attgctttga tttgttcttc ttcattgaat
     3421 ttcagcatga tttccacctc ttataattta cttttcaaga ctgtatcaaa gtcttcaaaa
     3481 atctttttgt tcaagtcaat acctttagct actgcggttt tataagcgaa tagttgaaac
     3541 ggaacgacaa gcgctaatac agacagatat tcatcacatt ctattgctaa tccaagtgtt
     3601 ttctcgtttc ttgctttttt tgtggtaatg gtgagagtct ctccaacgta ccgactcata
     3661 tatctttgca acttttgcga acgttcctta tttacacttt ccccttcaat aaagaatagc
     3721 aaatgcgaag catctgcctc caggtatggg ccgtgcatat aagcttctaa ctcaaatcct
     3781 tgtgacggaa cgcggattgt ttcagtcaat ttcgtctctg cttctttagc tgttccccag
     3841 tttggaccat atccaatggt gacgaaacgc tgtgctaaac gaaataaact ttgatatcca
     3901 tcaaaaaatt cttccgtttt ttgaataatc gctggtaaat ggttgattat tttgcgcaac
     3961 tgttgcatgt agtcttgttc tatttttttg ctgattttat ttttacttat tccaattcct
     4021 atccctaaga gtagtaactg taaaacagtc gcagaatagc cttttgtaac gaagcctact
     4081 gattctaccc cggcacctaa atcaataatt tgtgcagctt ctaaagcaag tggactttgc
     4141 acgtcatttg tgatagcaac aacaggtaac ccttgttttt gtatcatttt tactgcttca
     4201 acagtagaag cacttttccc gctttgtgac acagcaatca cagtatcgat tgaaggagaa
     4261 agttttccat aattgaaaaa ataaaaaggt tcttgaatat cgacagtaag atcaccataa
     4321 gattccaatg ctggcttcgc tgctaaacaa gcgttataag atgaacccgt tgctaagatc
     4381 agtaaattca ctgttttttt catatgttct cttgtcgaaa aatcattctt tttcaagatc
     4441 gtacttagta cttccttttc ttcatagata taatcttgca tcgtaaacat tttctctgct
     4501 ccttttttaa aaaatcccta tccacgaacc aaaaattcca aagaaagcta gtccgatcaa
     4561 gatcgttaat ggtttcactt ctttcttcaa tagataaaac actatgccaa atgctccaag
     4621 acataagata tttggaacga tatcatcaaa aatactttga acagtcacag cattttctcc
     4681 tgaaccgatt tttaaaggaa tattgatggt gatcatcgtg gcagtcattg ctccaacgac
     4741 catcagtcca ataatagacg cgcctaatgt caaactttcc atcataccgt ttgcctggat
     4801 ctttttcaaa aaacccgttc ctaagcgata tccccaacct gttgctagat agcgaaacag
     4861 aatgtgcgga atattgaaaa tcaaaatgaa caagataggg cctaaaatat ttccttgcat
     4921 ggctaaagag gtacccacac ctgtggcaat caatcgcaaa gttccccaaa agaaagaatc
     4981 tcctaaccct gcaagaggtc ccatcaaaga tgttttgata ctatcgatcg cagtcacatc
     5041 aaattcagga tcatccttat tttcttcctc cattgcagcc gtgattccta aaatcagtgt
     5101 aacgatatgt ggggtcgtgt taaaaaactc tagatgccgt ttcaaagctg ctgccatttc
     5161 ttctttcgac tgatataact ttttcaaaat cggaatcatt gcataaacat aagccatatt
     5221 catctgacgt tcataattcc aagaaaactc catttgaaaa gaacgccaaa atacgtgatt
     5281 tagctctttt ttcgttattt tgctgttttt gatctcagaa ttcttcatct tcttctacct
     5341 cccctgcagt tgtttgaagt ggtagttgac ggacattcat aatattgact accacaactg
     5401 ctaaaatcgc accaaaaata gctactccgg tcacaggaat ctctaagtaa gaagctatag
     5461 caaaacctaa gaaaaaatat ggtgcaactt gtttgttgat cagcaaacga gcaagcatcg
     5521 caaaaccaag agctggaatc aaccctgtag ctactgacaa ccccttttga acgaagtcag
     5581 gaatcatatt taatagattt ccgatcgtat tgcttcccac catataagaa accattacca
     5641 caacaccaag catcaaggaa agaccgatac cggcaagcaa gtgcatatgc tcgactcctt
     5701 tataatttcc ttcttctgca taattgtctg ctttatggtt cataattgga atcaaaagcc
     5761 ctaagtaaat atttttcaat atcaatgtca acgtagcaat cggcaaacct agtaaaagcg
     5821 cggtttctgt accggctcca gctgtaatag caaaagccgt ccctaaaatc ccaccggtca
     5881 caacatcagg tggaatcgaa gcacctactg agaacgatcc gataaatgct aactctaaag
     5941 ttgcgcccat aatgatccca gtttttacat cacctaacac gattcctgta aacagccccg
     6001 tcacaatcgg acgagaaagc aacgatgtcc ctagtgcata ttctgattgt gcgacaaatg
     6061 caacaattcc taataagact gcttgtacaa tcattgaggt tcttcctttc ttacaaaact
     6121 tctcttacat ccactttttt atctgctggt acttgacgga tttctacttc tatttgtttg
     6181 tcaattaatc gtttcaagcg ttcctcctct tctggtaaaa tatttaccgc ttttccgatg
     6241 ctgcgagtac caggttttgc cttgatccct cctaagttga tggttttgat ttctggatac
     6301 gcttctgcca gcttatatgc atcctcgatc gattccacta cgatgaataa cttatattta
     6361 tctgtcaccc cgcttttcaa cgcggcaatc gagtcatcaa ttgatttgat cactagtttg
     6421 actccttgtg gcttggctaa tttgatcgtt gtctttcgta tctcattcgt aggtacatca
     6481 tcattagcaa tcaaaataca gtctgcgcct attgtttgtg tccatgaaaa agctacttgt
     6541 ccgtgtaata accgatggtc gactcttgtc aatacaatca tttttcttcc tcctaaaatt
     6601 cgtcttcttc tattttctga ttgagccgct cgttacaaaa ctggatcatt ttttttgaat
     6661 cagttagcac ctgctggatc aaaacagttg tctctacttc agcgtccaac cgagatacta
     6721 gctcaatcaa tagtggcaaa ttcaatccgg caacgagata gacatgatcc cgattgatat
     6781 attgtaagaa ttcgttgttg acacttccgc caaaaagatc cgtcagaaca atgatttctt
     6841 tatctttgta cctcataaaa agctgcctta ctgtttccgc taaatcaaaa ttctcatcag
     6901 tatagcaatc gaatacagta atagaatgat ttttcccata gatcagctct aatgaatcta
     6961 atatccctga agcaaatttt ccatgggaag ctaagataat ttctctatcc actttttcat
     7021 ctcctctttt cacttacaca tactaaaaaa gcaagttgca tgccaaaatt agtggcaaca
     7081 acttgcttat gttcaaaatt cttcttctat cgagagaaac tctgtatctt cactcaaaat
     7141 atcatagata taggcaatct cagaattggg gatcatgaca ctataatgct cttcaatgac
     7201 actaaacgca gatttaatag cagataaccc ttctttttga cactgcttcc atttctctaa
     7261 tccttgatag gtttcgattg gcatttgtcg gatcaatcgt tcaatgagac agctcacatg
     7321 gacatacaac gctaattttt tgggattgct gatgcttcga ttcaactgca cctctaattc
     7381 tctcataaaa tgatcgattt ctcccatcac cttctctgta tctagaatag tcacggaatc
     7441 cagtactttt tccaatgaaa aattacgaat gatgcgctga ttgaatattt ccttctctga
     7501 agcattcata gtagtgtcca accattcagc tagtacagac atcttttccc ccgagatgag
     7561 gtcttctaat gaaagatagt cgatattggt gatatcagga tctgtggtgc caatcattcc
     7621 tacgacttcg tatatggaaa ataatgggtc actgtttttc ttatctttta attgctgata
     7681 ttcatatggg atgattttca actgacaagc cgtaggtaag cttttttcta gcagttcact
     7741 taagtggacc gccgtaccga ttccagtgaa gcaagtagta atgagtgctt tgggtttgtt
     7801 ttcctctgga tagagcagtc gccattctgg ctggatctgt cgaacagtct cttctgctat
     7861 ctcttctaat gatacatttt tttgcaggca ttcccctact gtgattgcta aaggtgtcgt
     7921 cacattattc aaaatcaaaa gcggtacgga taactgtttt ttaaaaaact gatggatttc
     7981 tttcagagat cccatgtcta ctaatatgat caatccattt gaaacatcgc tgttttcgca
     8041 ataatcaagg atttctgaag caatctgctg tggtgtgaca tctagtggca tatcaaatga
     8101 ttcaaataga tcctcttgta ataaacgatt tactacattt gccatactgc tagcagttgc
     8161 ataaccgtga gcagcaatca tcgcttttgg cagtcgtttt tcttttgacc atttcgcttt
     8221 ttttagatag atcgtcagta aaatccgatc catacttgtg tcttcaatat ctaatttgat
     8281 cttacttaat tccagaattc gttggacata gtgataacta gatggataag actgagaaac
     8341 ttgttgttct aattctttga ttagttggat aatatcatgt tcttccggta accatttgat
     8401 agtgcttcgc tgatagagat agtaactcaa ggcatagaca ctgtttccat cgaatttgat
     8461 ttgataggca gtttccattt gtcgaaaggt ctctcggata gattgtgtcc aataaaccag
     8521 cagctcgtgt ttttcttgtc tactggtctc aaacagcaaa aaatcaaata attgaatcac
     8581 ttcttgcttt aattttcctt cgcaagaata tagcagacag ttatttttct tgaaatcaat
     8641 taggatttgc tcaaaacttt taacgattcg ctgctgttcg ggctgttgct tctccattaa
     8701 ttgctctaag gtctggtctt gcgtaatata gattccttgc tgtgctgtat ttttagtaat
     8761 aggctgcgca agtagttctt tttgcaaatg atggatcgtg acatggattt caggctgttc
     8821 acgctgttca gcatatgctt ttgctgctga aactttgacg atatttttca gctctcctat
     8881 gtttccggat aatttctgac ttgttaaaag tgacaaagct ggtccactga ctgacaaagg
     8941 gctccccatt tttctttgtt cttcgagaaa aaaagagtaa atcagttcta gccgttcatt
     9001 acgactacgc tgattaagtg caggtagttc gattcggata ggtattcttc ggatgaaagt
     9061 tgttaagaaa tgactagtaa tctcttcagt cgtagcaaaa aagagacgtg ttttgatccg
     9121 gcgtgacaaa ttaggttctc ccatacgata aatcacacct tgatcaagat aggtgaacag
     9181 tttttcctga ccttctggtg ttaagcgatg tacctcgtct aaaaacaaga tcccaccatc
     9241 tgctgcttca aaagcccctt ttttattttc ttctgcacca gtgtacgctc ccttaacatg
     9301 tccaaacaaa ttgctagtca aaagttcagg attatctgca tattgtgcac aattaaaagt
     9361 gatgaatggt gcagaatctt ctagcagatc atgaagcaga caatactgat agaccaaatt
     9421 aactaaataa cttttacctg tgccactttc gcctgtaatc aaaagtggta atccgccatc
     9481 tggatagtaa agagctgttt tgatttgttc gatactttct ttcaaactgg cgtcgtatcc
     9541 gatcataaaa gagaataagt cttttggttg cgcgaaaaaa ggctgttcct ctttgagatc
     9601 atcgatagaa aaataaaaat tcgtgcgtaa cttaaaaaat tgtttttcaa aagcttcttt
     9661 gtggaaataa atcactggtc gagaattgat tttgactagt agttgctcat catttaactg
     9721 gttcaaataa tgactgactg tgtttctttt taccccaaaa tgatcagcta acctttgtgc
     9781 tgtaaagacc tcgttcatat gttctaaatc gaaaaaggct gtctggttct ctaagtaaca
     9841 taaaatttcc ttcttcaaca acttattcac ccctcctcca tcatagaaaa cggattcatt
     9901 ttgtcaataa aatctttaat aaagaattga aaaatatatc ttattataga cagcagccta
     9961 cttatccctt ataattttta ttctaaaaaa cgtacagttc cttccagttt ttatactgga
    10021 atttcaacat atgaaagaat aaggaggcta tgactattca atgaaatcgg ataaatataa
    10081 aaacaggaaa catacttcct ggaaaacttt tcgagaaatc atcggatctt atctcgctgt
    10141 agttgtattt ttgcctttgc tttatatggc tatgacagaa caaactcgtt ttattggcgt
    10201 agatacgcaa tatatagccg aaacaggatt tttattcatc tttatcttga tttttgcgtt
    10261 ctataaatcc ttccatacaa aataagattc acagatttca actttcctgt ctgtttttag
    10321 ctattacaaa tccttatatg tggtataaca acaatttatt ggcataatga ggaaaaacga
    10381 gttatgatgc tttcagagag taaagaggta ctgttgtcac catccttttt attgattacg
    10441 tacaccactc ttagtttttt cccattgatt acgaaaacaa ggtagaaaca ttgatgttcc
    10501 taccttgttt tcttttttta ttttcagatc aaaatctcaa gtagcttttg acttgatgtc
    10561 gggaggttgt cttcgttcca tcaaacctga tagaatctgc gccaaaccat agttttctcc
    10621 actattattt attcctctaa gatagtaccc acaataaacg aataaatatt tttctcattt
    10681 tcatactcct tctttgaaaa tcaattagat ttctctcaac ccactttttt acaaaaatta
    10741 cgtagcaaaa aagattgcaa gaacaaatta aaaaagaagt aaatagagtt tcatctataa
    10801 acatttactt gatatatttt ttatgcagaa ttcatttata gaaaaattaa gtaaaataga
    10861 cgttcctatg aatgaagaga ctggcatgtt ttctcatttt ctttttttta aaaaaaatag
    10921 tctattcaat ttctaaagct tcttcataat aaaactttca tttatgaaaa ggcttgttct
    10981 cttttcattc taaatttcct aagctacaat gtggtagtac ttgttttttc aattgtggac
    11041 aaataaataa taggaggaat catatgattc agttattacg ttatgcaaaa gactatcgta
    11101 agcaaatcat tttgggcccg ttcttcaaat ttcttgaagc tgtcttcgaa cttgtcctgc
    11161 ctttaatgat ggcttccttg atcgataatg gactaaaaat gaatgaccga ggaaaaatca
    11221 ttgaaatggg cttatggatg gtcgctatgt ctgtgattgg tctgatttgt gctatcattt
    11281 gccaatacta tgcttcagta gcttcacaag gatttggtac agagttacgt aatcagctaa
    11341 tcaaaaaaat caatactttc tctcacaacg agttgaatca ttttggaaca gataccttga
    11401 tcacccgaat gacaaacgac atcaatcagc ttcagctagc tttagcaatg gtcattcgtt
    11461 tattgatcag agcaccgttt ttaagtatcg ggtcagtggt catggctttt gtgatcgact
    11521 gggaagtagg tctgtttttc ttagcattat tgcctatttt ctcaatcatt ttatttttta
    11581 tcatcaaaaa aaccgttcct ctctaccaaa aagtgcaaga aaaactagat cagctcaatg
    11641 aacacgttag tcagaatctt agcggtgttc gtgtcatccg cgcatttgcg cagacaaaaa
    11701 aagaaacaga gaagttcgat aaaagcacaa atgatctagc gaacaattat ttacgtgttt
    11761 ccaatatctc tgccctatta tcacctgcta caaccttgat catgaatgtc gggatcatct
    11821 gtttgctgac agttggcggg atcaaagtaa atattggtag cttgcaacaa ggacaagttc
    11881 ttgctttgat caattatatg aatcaaatgc ttttggcact gatcgttgtt tcaaacctcg
    11941 tcgtgatctt cacacgtgcc gaagcttcag gaaatcgggt gaaagaggtg ctagacacag
    12001 aaaattcgat tcttgaagct gaggtcagta ctactcccga tttttcttct gaagcaatta
    12061 tcgcttttga ccatgttgac ttccgttata ctcctgattc tggtctttct ctccaaaata
    12121 ttacatttca gctgaagcga aatacagtcc ttggaatcat tggtccaacc ggaagcggga
    12181 aaacgacttt gacacaattg attccgcgtt tctatgatgt cagttccggt acagttataa
    12241 tggacggaat ggatgttcgt tcttggcagc tggatcatct ccgtcagcaa attgctcttg
    12301 ttccccaaac ttctgtacta tttaccggga ctattcgtga aaaccttcaa tggggtaaga
    12361 aagacgccac agaaaaagaa tgttgggaag ctctgaaaat cgctcaggca gaagaatttg
    12421 tccgtcagct tccaaatggt ttagatacaa aagtcatgga aaatgggaaa aatttctctg
    12481 gcggacaaaa acagcgactg acgattgcaa gagctttgat tgccaagcca gcattactga
    12541 ttttagatga ctctttaagt gcgttggatt atcaaacaga cttggatttg cgtaaggcgc
    12601 tacagactta tcttggcagt acagtgatta ttatctccca acgtgtccgc tctattcaag
    12661 aagcacatca tatcttagtg atggatcaag gtaagattgc tgctcagggt acccatgaag
    12721 aattacttgc tcaatcagca gaatatcagg aaatcgtcgc atcacaggag gaagaatagt
    12781 tatgaaagta aaaaaattat ccttgcagac gctccgccga ttcattcctt accttactgc
    12841 ctatgcaaaa gacatttggc tagctgtgat actaggaatc atcaatggga ttgcaacagt
    12901 tgtgatgacg ttacaaattg gtcaaagtat cgatcaaatg attggagaag gacaggtccg
    12961 cttcaatcga ctgttccatt tacttattct attcggtggt atcgttttaa tcaacgtagt
    13021 cagtcaatgg ctcatccaag tgttaagcaa tcggttagct tacttatccg ttgcaaaatt
    13081 aagaaaagat gcatttgctc atctgaatcg attgccactt cgttattatg atcaaaactc
    13141 ccacggaaat atcgtcagtc gatttacaaa tgatatggac aatatttcca ttgcaatttc
    13201 tgcggccttc aatcaattat tttctggagt ggcaatcatc attctttcgt ttatttttat
    13261 gatgcgtcta agtccacttc tgacattggt cgttctttgt tctacaccaa ttattttcct
    13321 tgtgagttgg atcgttgctc gtgcttcaca aacaaatttc gatagacagc aaaggattat
    13381 tggggatatc tcgggctttg tgaccgaacg aatcggtaac cagaaaatcg tgaaagcttt
    13441 tcagcaagaa gaagctagtc aacagcaatt tgaactgcta aatgctgatc tatatgaaaa
    13501 aggacaacgg gcacaatttt cttcttcctt gacaaacccg tcttctcgtt ttgtagatca
    13561 ccttgcttat ttatcgattg gtttagttgg cggcctattg gcattgaaga gtggttcgtc
    13621 aatcactgtt ggtgtgattt ccagctttac gatctactca agtcagtttt ctaaaccatt
    13681 tatcgaactt tctggaatca cgacacaact gcaaaccgct ttttctggtc tagaacgagc
    13741 atttgatttg atcgatcagc cagaagaaaa gcctgatttg ccagatgcct atgccttgga
    13801 acccaaaaat atcaaaggaa aagtctcttt tgatcatgtt tctttttctt atgaccccaa
    13861 gcagcgtttg atcgaagatt tcaattttac tgcttatcca ggtcaaacca ttgctatagt
    13921 aggaaaaaca ggggccggaa aatccacact tgttaatttg ctgatgcgtt tttatgacac
    13981 aacaaaagga acaattacag tcgatgacca tgacatccgc tctatccaac gtgatacatt
    14041 acgtaaaagt tttggaatgg tgttacaaga tacttggcta tttgacagta cattgcgaga
    14101 aaatcttcgt tttgggaatc cggaagcaag tgacgcagaa atcaatgatg ccctaaaagc
    14161 ctcttatatg tatgaatttg tagaacgttt gccggaaaaa ttagatacta agattggcgg
    14221 tcaagggtta aagatatcag atggacaacg ccaactgttg acgattgctc gaacgatgat
    14281 cagtaatccc cctatgttga tcttagatga agctaccagc tcagtcgata cattgacaga
    14341 gaaaaaaatc caacaagcct tcttagcaat gatggaagga aaaaccagtt ttgtgattgc
    14401 ccatcgcctg tccacaatca aaaatgcgga ccagatatta gtgatggatc agggagctat
    14461 tgtcgaaatg ggcacccatg atgagctgct tcaaaaagat ggttattacc gacggctcta
    14521 tcaagcacaa ttcacagcac a
//
LOCUS       DAXWLG010000059        14513 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXWLG010000059 DAXWLG010000000
VERSION     DAXWLG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.059"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(296..1024)
                     /locus_tag="ACGE4T_001424"
     CDS             complement(296..1024)
                     /locus_tag="ACGE4T_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386708.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACGE4T_001425"
     CDS             complement(1002..2576)
                     /locus_tag="ACGE4T_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8386709.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACGE4T_001426"
     CDS             2805..3743
                     /locus_tag="ACGE4T_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8386710.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACGE4T_001427"
     CDS             3755..4666
                     /locus_tag="ACGE4T_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386711.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACGE4T_001428"
     CDS             4690..6162
                     /locus_tag="ACGE4T_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8386712.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACGE4T_001429"
     CDS             6213..6824
                     /locus_tag="ACGE4T_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8386713.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACGE4T_001430"
     CDS             6854..8767
                     /locus_tag="ACGE4T_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFF8386714.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACGE4T_001431"
     CDS             8895..11876
                     /locus_tag="ACGE4T_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFF8386715.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACGE4T_001432"
     CDS             11873..13534
                     /locus_tag="ACGE4T_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFF8386716.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACGE4T_001433"
     CDS             13574..14506
                     /locus_tag="ACGE4T_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFF8386717.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4704 a         2289 c         2917 g         4603 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat atcaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa att
//
LOCUS       DAXWLG010000060        14447 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXWLG010000060 DAXWLG010000000
VERSION     DAXWLG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.060"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            174..350
                     /locus_tag="ACGE4T_001434"
                     /pseudo
     CDS             174..350
                     /locus_tag="ACGE4T_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(526..1095)
                     /locus_tag="ACGE4T_001435"
     CDS             complement(526..1095)
                     /locus_tag="ACGE4T_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8386718.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1169..2458)
                     /locus_tag="ACGE4T_001436"
     CDS             complement(1169..2458)
                     /locus_tag="ACGE4T_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386719.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2785..3660
                     /locus_tag="ACGE4T_001437"
     CDS             2785..3660
                     /locus_tag="ACGE4T_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8386720.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            3674..3997
                     /locus_tag="ACGE4T_001438"
     CDS             3674..3997
                     /locus_tag="ACGE4T_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386721.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            3994..4746
                     /locus_tag="ACGE4T_001439"
     CDS             3994..4746
                     /locus_tag="ACGE4T_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFF8386722.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            4736..6907
                     /locus_tag="ACGE4T_001440"
                     /pseudo
     CDS             4736..6907
                     /locus_tag="ACGE4T_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7019..9190)
                     /gene="copB"
                     /locus_tag="ACGE4T_001441"
     CDS             complement(7019..9190)
                     /gene="copB"
                     /locus_tag="ACGE4T_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFF8386723.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9210..11396)
                     /locus_tag="ACGE4T_001442"
     CDS             complement(9210..11396)
                     /locus_tag="ACGE4T_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8386724.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11396..11605)
                     /gene="copZ"
                     /locus_tag="ACGE4T_001443"
     CDS             complement(11396..11605)
                     /gene="copZ"
                     /locus_tag="ACGE4T_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFF8386725.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11618..12058)
                     /locus_tag="ACGE4T_001444"
     CDS             complement(11618..12058)
                     /locus_tag="ACGE4T_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFF8386726.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12132..12630)
                     /locus_tag="ACGE4T_001445"
                     /pseudo
     CDS             complement(12132..12630)
                     /locus_tag="ACGE4T_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(12915..14381)
                     /locus_tag="ACGE4T_001446"
     CDS             complement(12915..14381)
                     /locus_tag="ACGE4T_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8386727.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4113 a         3031 c         2562 g         4741 t
ORIGIN      
        1 attgctgaat ctcttgccaa ttcaacgtat actttatagt tcttttcaaa agtttcttta
       61 tcatgtgtgt gttgtaactc gatttgcaca ggacttttgg ctcacgaggt tgtcctactc
      121 tacatcttgc tgatagatat gctgaaagga aaacaccaac attatgtttt gctttttcag
      181 ctagaacgag acaagcagga atacctttag aagatatcaa agatttttta ggtcataaag
      241 atgtttccac tacacaagtc tatgcccata tttctcccga agtgaaaaaa agatcaatga
      301 atcaactaga aaactatata gaagagcaaa taaaaaagca ctcaaattga gtgctttttt
      361 aagctcacta tcactaaaac tatcactaat tttatttata agcgaacatg tttctatcac
      421 tgatttttta aatctagcga aaaaccctta tcttaaaggc gttcgttcaa ttctttggca
      481 aggtcttcaa agcctggctt gcctagaagt gcgaacatgg ttttcttata gctcatgtag
      541 aaaaattttc ttgatgatga ttttatccat caattgagat tgactgtttt gctctcctat
      601 gatttcagct atgattgtcc gattttgcgg ttttcttctt aactggtagt tgattttccc
      661 agtttccatc aactcgctaa gcatatccac gactccgcca aatccggtct ttttcgtaat
      721 aaaacctagt gctaccattc gttcatctag attttttgct gtaatcgtca caccagtaat
      781 gagcgagtgc tgtaatgttt gggccagctc ttccatccct tcagcattgg atacgacgga
      841 agctgttttg ttaataggtt cttcgtttat ttcttttggt tctttcctct ccactttcac
      901 tgtttgacga aggacatcta gttcttgcaa gagcgttttg tattgttctt gggtcagcat
      961 cacgccagct actttttcac gattaaatat atatacgcca gttcccgcgt ctcgcgcttg
     1021 agcaaataca tccatcggcg atcgtttcac ttctgtgatc gaagatgtgg gaacttctaa
     1081 tttctttaat tccaatggat ttcctccttt actaatcgct ttttattata gcacagactg
     1141 acttgttttg atcttttttc caaaggtttt atcggttttt gcctaaccag taattgatat
     1201 cggtaagaag tctttcgata ccaaatgaag gatggtacag atacgttttt tgatcttcgt
     1261 tatggtctag tggaaaaaaa tgatcggtga tcactagatc tgcataccga ggcggcttat
     1321 ttcgagtaca tacatgtata gagatccgtt ttcgatgtct tagcgttttg ttcagtaccc
     1381 cagcaagtct tcttgcttct tggtgttcat agcgaaaaaa aaagacgatt ttcacttcag
     1441 gagggtggac atctaaatac cctcgttcct ggagtgtttt cagtaaaagc atcccccact
     1501 ctactgcggg gtgtgcaagt gcttgttgga tcgctagccg aaattgatat agtttatctt
     1561 tggaattggg aaatttttct aatgtttcct gataataacg aacttcttct atcagccggt
     1621 catggatacc aattacgtaa cgaagccatt cttggtaaag tacttttatc agctttgatt
     1681 tgctttcgct ctcaaattcg ctattcagcc aaaacagcga atcaagcttt tcagtcacaa
     1741 tcgtcaattt ccgatagtct tgtgtctctt ctatccgttt ccaccgcttt tcagtaatct
     1801 ggttacagct caagcgcaaa attgctagat aatttttttc ttttattggc aaaatctgct
     1861 cctctctaaa tagcaattca ggaaacaatt caaagaatag tgttcgactg tctcctaaaa
     1921 tctcgatttc tgccggatcc tttctttgct tcaataattg gatgaacatc tcagaaaagg
     1981 actgctctcc atcatctgca aaaaaatgcg gaaaatcttt caaaaagtaa aacagcagat
     2041 atcttacttt tctttctgaa ccaaagatct gtgctttttt ataagaaaaa gaaaggtggt
     2101 actcgccaaa ccactttctc aatcgctctt gcgtagaacg atcgtacttc tgctcgtaag
     2161 gttcatttaa tagcagattt ttcagtaaac aaaacggggg tgattggatc agaaaaacaa
     2221 acaagagata aggttcgcta tatccttgat cataaactaa ctgaacggtt ttcttatgaa
     2281 gaatcagctg aatgggacag tcgctttccg ccaaacgttt cagatgccga tccactgttt
     2341 taacatcgat tttcagttct tcagctaaat cagcttttcg ccagatcaaa tctttcatta
     2401 gattgattag tgaaaataaa ttctgtgcat tttttgataa tagtttttgc catctcatcg
     2461 atcatttctc acctgatttc taaacatatt gtttgatcaa aatgagttca ctcctaaaaa
     2521 gataaaaaac tccctttcta tttatttaaa tacgcaaatc ttcctcatta ttttttattt
     2581 tactcaattt aatcgtattc tcatttcaaa acgaatgtac tttaaaaaaa cataataaga
     2641 tcactttgct tgtaggatgt attcccttcc attacaatga aataaatagc gattttatga
     2701 gaaggtgatg tgaaaatgga agaaaaaata gaagaatcca aacaaaaaat cgatcagttg
     2761 atataagaat tagaaaaagc ggtcatcggc aaacgttcaa ctatccaatt gatgatcgtg
     2821 gccctattag caggtggtca tgtcttattc gaagatattc ccggtgtagg taaaacgtta
     2881 ctggtcaaag cactagcaaa agctgtacag ggaaccttca gcagagtcca atgtactcct
     2941 gatctgctgc ctagtgatat tcttggtttt tccgtataca attcacaatc aaaagagttt
     3001 gaatttcgtc cgggaccgat ctttaccacg atcttgttgg cagatgagat caatcgtacg
     3061 actcctcgta ctcagtcagc acttttagaa gcaatggccg agaatcatgc aacgatcgac
     3121 aacaatacat accctctaga caatcatttt tttgtcttag ctacgcaaaa tccaatcgag
     3181 tacgaaggaa catatccttt gccggaagca cagctcgatc gttttttatt ccgactacaa
     3241 atcggttatc cttcttttga tgacgaactt ttgctacttt tggataaatt cgaaaaagtg
     3301 ttggataact taaatgaagt attgaacagt tatgaaatag aagaaataaa aacaaatgtg
     3361 gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct tgcagttagt gtcagcaaca
     3421 agaagtcatg ctgccatcca gcttggcatc agtccacgag gaagcctttc ctttattcag
     3481 gcagcaaaag cttatgcttt aatccatggg agaaattacg tcacgcccaa agatttgcag
     3541 gatttagttc cttacgtgtt ttcccatcgc ctgatttttt acgatcgttc attgaaggaa
     3601 gacgaaaaga aacaaatcat tcatactctt gttgcacaaa ttcctattcc tgtgaggtag
     3661 attgaggtag acaatggaaa aaatcaaaga ttatttcatt aattttgtgt tatttctggt
     3721 atatttcatc attgtgcttt ttgctcttgt attcaacaat tctttaggct ggttcttgtt
     3781 ggcctttttt actctttatg tatcatggag cttgatttca ttaagcagct ttctaaagaa
     3841 actttcggtt acttgtacat tgcagccact cgtttatcgc aaaaaaagta tgcaggttat
     3901 tttttccata gcaaaaaaga gaactttttc tttacctctc cctcgtttac aaatcggctt
     3961 cccagcaatc tttgcagaaa aaaacaagag attttgattc ttacaaataa accgcaagaa
     4021 gtccaattgc tttggcagcc aaaagaaaga ggatgccatg aaacattaac tgtcattttt
     4081 acagcctatg atagtctagg acttttcaga aaaagtgcaa aaagagagat ctcttttcct
     4141 gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa aattacagag agttctggaa
     4201 aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt ttcgggaaca tcgcgcttat
     4261 cgtccaggtg attctttcaa ccgaatcgat tggaaattgt ctgctcatac tcaagagtgg
     4321 ttataccgtg aatacgatta tcaagaagaa gaatgttctc ctcttttttg cttttggggt
     4381 tcgcccagtc caaaatttga acctctgcta gacttgtatt tttctttatg gcatttgaga
     4441 aaagaaaagc agccggaact tctgatatta ggcaaccgcg cctttcatgg caaagacccc
     4501 ggtcatacct attttgcttc tttggaagct ggcgatgaga aagctttttt cgcacacttg
     4561 aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg agaattctcc tgaagtctca
     4621 gaagctattg aaacattgtc aaaagatcga acggcttatc ttgtttattt cgtagaaaaa
     4681 caactcatgg tccaagaaaa agacaagata tgttcattgc caggaggtga atggattgac
     4741 gattaaacgg gaaaaatggc tgcgtggcgt ttactccttt gtgatactaa gtgtaggtgc
     4801 tccgatattt gtagtagcta atcaattgca aaacagctac cttcttatcc taactattgg
     4861 tgtcctatgc cttattgtct gctgtattcc taaaaaatgg gtacgtttgc tgctttcttt
     4921 gccagtaatg ataggttgtt tgtttttata ttttccttcg aagacatttt ccatattatg
     4981 gatcttacag tttttttcgt cagttacaga ggaattcttc catctatccc aaggtaaact
     5041 ttattatttg ccagaaaaga cagctttttt tctcataatg ttgctgatct atttatttat
     5101 tacattgacc gtggattacg actattggta cgcacccttt ctttctttga tggcttactt
     5161 catgatgctt accgtttttc ggaaaaaaga tttactgtac gaaatgattg cggtagtaac
     5221 tgttttgttc gtgtatctgg cagcaaggca atttttctcc atccggttgt tgtcgggaaa
     5281 cactcgattc cagtcattga ctacagttct gcttatttgt ttcttaactt ttagcgctat
     5341 cttgccttta tatcttccga aagtacacca acctctcgag gaaacgagtg aaccgatccg
     5401 agaatatctt aatgatgaag ggctttatga cacactccat gagtatcaat taaccggaag
     5461 tgcaagaaca ggcttcagtg agaatgatga acagcttggt ggacctttat atgataatgg
     5521 cgaagttgtt tttacagcga accaaaaagg aaatggctat tggaagatcg aaaataaaaa
     5581 tcattacacc ggttctggat ggatagaaga tcaggacgca cccatccaac caatccaaga
     5641 attccctgtt gacatcacga gctaccaaac cgttatagaa gacaaagaaa acattacatt
     5701 gaccctaaaa aaacaagctt tcttacctta tccttatgga aatactacct tgactgattt
     5761 ttcattagct agtggacaaa cgctttacta tttgcctgaa agccagcgat tcattcctgt
     5821 cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa aggttagttt ttacgagaga
     5881 agaattaaaa agcagtcagt taaaagctga tgatttagaa aatcagcaga caaatcttca
     5941 attgccaaaa agtttgccta aacgtattga ggacttggcg gtcgaattga ccaaaaatga
     6001 agcaacttta attcgtaaag tcgaagctgt ctaacagtat ttaaaaacaa gcggaaattt
     6061 ccgctattcc aaaacggata ccgggcatac tccgaaagaa cacgattatg tggattattt
     6121 cctctttgat tcaaaagtcg gctattgtga caacttttca acttctatgg tcgtccttct
     6181 tcgaacactt ggtatcccag ctcgctggac aaaaggtttt acaaatgggc tcagaacagg
     6241 cacagataat caagggaatg aggaatacag cattctcaat agtcatgctc attcttggcc
     6301 agaggtatac tttcctggat ttggctggct tccatttgaa ccaacccctt catttgccaa
     6361 tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc gtccagcctg ctatcgaaca
     6421 accaaatgaa agcgaaaata tggcagaaac tatttccagt gagagatctg aggtcattcc
     6481 atcagaagaa acaacactca caccagctta taaaatcgtc ttattcagcc ttggagtgtg
     6541 tttatccgtc ttgttgttct ttctattgtt attcagaaaa tctctgcgct ggacaagtta
     6601 tcttatctat cttcattttt ttgcttctac taatacgatt gcatatcaaa aacttcttaa
     6661 ccctttggaa acattggtac cgcgcaaacc cgcagaatct ttgcaggaat atagcctgcg
     6721 aatagaacaa gaattcaagc tagatctgcg tttcattcga ttaactactc aatatgaagc
     6781 agaaatatat ggacgacaag cagatccggt aaaattcgat aaaaaaacct atttagaaat
     6841 aacgcgtata ataagaaaaa cggtatacaa aaaaattctg acaaaaggga gccgcttcaa
     6901 aaaataagat aaaatttctg gagaatctac ttctggaatg ccgagaacca gctgattgag
     6961 tatcgattac tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt
     7021 atttgatttt caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga
     7081 cagcaccaac tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg
     7141 gaattgcaag aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg
     7201 tttgcttggc tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga
     7261 catccgcaga atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac
     7321 gggctaaact tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt
     7381 caaggtattt ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt
     7441 cgatccctaa ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga
     7501 caggagtgat ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta
     7561 cgtcacctaa agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat
     7621 ttccttgttc ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa
     7681 cttctttttc attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc
     7741 ctgaaagatt ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat
     7801 tcataatgcc aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa
     7861 ggatttcatt tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc
     7921 cttctgttag tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca
     7981 gtgcattgcg gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gagcgagcaa
     8041 caactaatgg gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca
     8101 tacgttctaa tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac
     8161 ccacaattaa tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct
     8221 tggatttttc tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt
     8281 caccagttcc agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca
     8341 ctttgtcatt ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa
     8401 caattgtttc acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga
     8461 tcaaacgatc accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg
     8521 tcaagcgttt cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat
     8581 tcgacacagc attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca
     8641 attcccagaa gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat
     8701 agacactata aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac
     8761 ttttttgttt caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata
     8821 gtatagttgc taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa
     8881 attccattcc catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc
     8941 aaaatttttg tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat
     9001 ccatgtgcat cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca
     9061 tgtgcatgga atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag
     9121 attctgtact ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt
     9181 tcccattcat ttttactcca tcccttctct tactttttca tgtgtcgatt taagctcagg
     9241 gagttcaaca atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc
     9301 aaaaagccta gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga
     9361 ttttgtttga tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc
     9421 aaactgctat tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc
     9481 attgccatac cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc
     9541 ataccgactt ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga
     9601 agtacttcag caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct
     9661 gccagtttgt tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg
     9721 attgcttgtt tggcttctag tttgatttga tcagccacgg caatccaccc gatcacttgc
     9781 ttttcatcag ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct
     9841 tggtattcat caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag
     9901 cgtactccat taatcgtacc gctgatccca gcacctgggt gggctgtaaa atcaatcata
     9961 tcgtatgttg ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt
    10021 tcagaagctt gttctaaagt ataaaaaata gataggactt cttttgaacc gactagatct
    10081 gtaactttgg gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag
    10141 tgagcagctg cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca
    10201 cccgttccta ccataatagc tgttggggtc gctaaaccta atgcacatgg acacgcaatg
    10261 actaatacag agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta
    10321 gctatgagcg tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga
    10381 tctgcgattt tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt
    10441 tgcgctaata ctgtctgctt gccgacttgt gtgacttcca tgtgtagcag accattcgta
    10501 ttgattgtcc cgccaaaaag cgtgtcatcc ggattctttt ctacaggaag attttcacca
    10561 gtaagcatac tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc
    10621 tgctcaccag gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt
    10681 tcttctttcc cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg
    10741 attgcattac ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc
    10801 gtgatgatca tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg
    10861 ttgtaaatgc tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt
    10921 ggtgctttcg tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat
    10981 tggacaggag cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc
    11041 gcatggcttc ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta
    11101 ctcaatataa gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt
    11161 tgtttgtggg cttcatcgta caaaattgca ccataaccga tgttttctac gctttggatt
    11221 aatttttcag tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact
    11281 gtggcattga ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt
    11341 gcacaagtca tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa
    11401 tcacctctgc tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt
    11461 ttgtttcatc aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca
    11521 cgccgtcaag tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac
    11581 cttcgattga aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac
    11641 attgacctgg gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt
    11701 tcaaaatctc attgatatct tcttgagtca aagtagcttc ttctactaga tccgcaatcg
    11761 tctctccaat cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg
    11821 tttccatctc cgagacagct ggatgataaa tgaatttttt tcctttttgt tctgtccaga
    11881 tcacttcttt ttttaccaaa cgtcccagca aagttttgat cgttgctggt ttccaatcca
    11941 tcgaatcagc taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa
    12001 cacgcatgat ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt
    12061 atttcacacc tttcgtttac aaatgtaatc attagcttat gaatacagat tacgcttgta
    12121 aacaaaaact gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc
    12181 tctcgtcttg ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc
    12241 tgtatttttg gttcattgtt tccttcaaaa tgccagataa aagataaaaa ttctaagtat
    12301 ttcctgttta gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc
    12361 tttcttttta gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc
    12421 caaatgatct tatctgcttc tttaatcctt tcctctaacg tagaagtata atttccttca
    12481 acgatccatg acggattaga tactataaac gattgctgtt cttgcaaaaa ccactttttt
    12541 gcctgaatgc ggaaagccgg tgatttcttt gatcttcctt gcgagggacg attttcccga
    12601 cccactttgt ccaatcacga tgatcttcat tcttctccct cacttctctt tggaatttgg
    12661 ttcgatcata ccacttataa cgccgcaaaa aaacaaggga ctgtgacaaa agtcgtgtca
    12721 aagtcccttg tttcgaataa atggtgttca atcagctgac cttcagcatt agctcaaaaa
    12781 acggcaaaat atgaggagct atttttgttt ggctgttctt attacttgat gcaggacagc
    12841 tttttcacaa cctctgattc acggtgttcc agaagtagct tctgtcacaa cctcttgtaa
    12901 caagtatttc tattttaagg ctgaaccact gtttctggtt cagtaatttc tttgtttttg
    12961 tctcgacgag caattgctgg caagatcata cctaataaga tcaagacaat tggtgtgatg
    13021 atattcgtga ataattggaa ccaccatgct ttaggatcag cagtataatc tacttttggc
    13081 accattccta ggatacatgc aaacgcagtg aaggcaaagc accaagcacc aaagataaaa
    13141 ccgatttttg gatttttaac aaatttatat tcagaattga atttcttata agctttattc
    13201 aacatcatat atgccaagaa cacccatagg taacgcattg gcatgacaac tgagttcaag
    13261 ttcgtcaacc attttactaa ttcattcata tctttgatac caaagatcgg caacaaaata
    13321 atgattccta ctagtatacc tgtcagaaga taaccattca ccaatgtacc tttacgatta
    13381 cgtttacgta gccagtttgg tacaaattct ggatccgcat ctgctaacaa gatttttagt
    13441 ggtgcatcaa ttgaaaaggc taatgcggag atttgtccaa tcatgtttgt caatgcgtaa
    13501 atgatcatca atgagttacc gataccatag taattaccta aaatttgaaa agcactgtat
    13561 gccccatttg ccatcaagtc tttagggata ttatcgctag caaacagcat acccattgcg
    13621 actgagccta atacagcaca aatccctacc attgctgcta agaagatcat ccctttagga
    13681 aattcctttg caggatttct tgtagagttg acataagggg atattttttc cgcccctcca
    13741 actgcaaata ctagcatcga aacggttgtg aaataactga aatcaaatgt tggaatatat
    13801 gttttcactt gattcatatg tggtgtggca aattccatcg ttggattgat gaacggtgcc
    13861 gttacagcta aaagaataaa taagatcgac atgacgaaca tcgctgttcc ggccagtccg
    13921 ccaatcactt tcaaagtaga aagtcctttt gttgataaat ataaaaaggc aagaaatata
    13981 ataagtgaaa taactgctac catttgtact gacatcgtat ttacgatatt tccatttcct
    14041 tggactgccc aaccaccagc aatcagaatt gcttgaggct tttgtgccaa ataaggaata
    14101 tggacgaccc agtaagtcca agcggcatag tatgccagtc gtttattaga tgtcgtattt
    14161 tcgacccatg aactgacgcc tcctttacta tccttgaagg ctgatcctaa ttgtccaacg
    14221 atcaatgcgt atggaataaa ataaatggcc aaaatcaata gccaagaggt gacaactgat
    14281 actccttgct gagcatagtt attaactaca ttccccatgc cccaaaccat gttaaatgca
    14341 attaacgcaa cagtaaacca gcgtaattgt ttgttgttca ttcgttctct cctttatgat
    14401 aaaaatttgt tatccagatg taatcatatt caatttttaa caaactt
//
LOCUS       DAXWLG010000061        14432 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXWLG010000061 DAXWLG010000000
VERSION     DAXWLG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.061"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(54..1283)
                     /gene="rpsA"
                     /locus_tag="ACGE4T_001447"
     CDS             complement(54..1283)
                     /gene="rpsA"
                     /locus_tag="ACGE4T_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFF8386728.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(1418..2098)
                     /gene="cmk"
                     /locus_tag="ACGE4T_001448"
     CDS             complement(1418..2098)
                     /gene="cmk"
                     /locus_tag="ACGE4T_001448"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFF8386729.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(2147..2782)
                     /locus_tag="ACGE4T_001449"
     CDS             complement(2147..2782)
                     /locus_tag="ACGE4T_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFF8386730.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(2848..4251)
                     /locus_tag="ACGE4T_001450"
     CDS             complement(2848..4251)
                     /locus_tag="ACGE4T_001450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFF8386731.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(4238..5269)
                     /locus_tag="ACGE4T_001451"
     CDS             complement(4238..5269)
                     /locus_tag="ACGE4T_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386732.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5300..5521
                     /locus_tag="ACGE4T_001452"
     CDS             5300..5521
                     /locus_tag="ACGE4T_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFF8386733.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(5686..6423)
                     /locus_tag="ACGE4T_001453"
     CDS             complement(5686..6423)
                     /locus_tag="ACGE4T_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFF8386734.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(6576..7916)
                     /locus_tag="ACGE4T_001454"
     CDS             complement(6576..7916)
                     /locus_tag="ACGE4T_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8386735.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(8153..9676)
                     /locus_tag="ACGE4T_001455"
     CDS             complement(8153..9676)
                     /locus_tag="ACGE4T_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8386736.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(10054..10665)
                     /locus_tag="ACGE4T_001456"
     CDS             complement(10054..10665)
                     /locus_tag="ACGE4T_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8386737.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(10788..10913)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(10995..11711)
                     /locus_tag="ACGE4T_001457"
     CDS             complement(10995..11711)
                     /locus_tag="ACGE4T_001457"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8386738.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(11717..12286)
                     /gene="scpB"
                     /locus_tag="ACGE4T_001458"
     CDS             complement(11717..12286)
                     /gene="scpB"
                     /locus_tag="ACGE4T_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFF8386739.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(12270..13040)
                     /locus_tag="ACGE4T_001459"
     CDS             complement(12270..13040)
                     /locus_tag="ACGE4T_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFF8386740.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(13037..13408)
                     /locus_tag="ACGE4T_001460"
     CDS             complement(13037..13408)
                     /locus_tag="ACGE4T_001460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386741.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(13427..14314)
                     /gene="xerD"
                     /locus_tag="ACGE4T_001461"
     CDS             complement(13427..14314)
                     /gene="xerD"
                     /locus_tag="ACGE4T_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFF8386742.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4043 a         3120 c         2449 g         4820 t
ORIGIN      
        1 aaaaactgag acaaaagcag ctgctttcat ctcagtcaca gatacaaaat caattatttt
       61 tcagaatcat cggttgcttt ttcttctact tcttctttca acgcatcacc taaaatgtct
      121 cccatagtaa atccagtatt ttcttctggt aattcataag actcaacatt ttttggttct
      181 tcttctgaag ctggtttttc ttctaaagct ttgatactca atgcaatacg atgttcttct
      241 ggatggatct ctaatacttt cactttcact tcgtcacctt catgaagcac ttcatgtggt
      301 gtcgcaatat gtttatgtga aatttgagag atatggacta gcccttctac tccagggaac
      361 acttcaacaa aggcaccgaa gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca
      421 actggagctt ttgtatcgat ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag
      481 gaaacacgtt cttgttcagg attgatagac aacactttga catttacatc atcattgaca
      541 gccaatacat cacttggttt tgctacatga ctatgtgaaa tctctgagac atggactaga
      601 ccgtcaattc cgcctaggtc gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca
      661 ctgagaacat ctccctcatg gatagttgaa agaatttctt ttttcttttc ttctctttcg
      721 ttttccacta ccgctttatg cgataagatc aaacgatttt ctgatggttc gatttcgatg
      781 attttaaagc tcaatgtttt gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc
      841 accattgatg cagggacaaa accacgtaca ccgacgtcaa cgactaaacc gcctttgaca
      901 acatttgtaa caggtgcttc gatgaccttg ccctcttgga aatctctttc gatatcttcc
      961 caaacttttt tagcatctaa acggcgtttt gaaagtaggt agctgccgtt ttctttatct
     1021 ttgccgatcg tactgataac gactaaatct aatacatcac cgacttttac tacttcgctg
     1081 atatcttcta ccggcaaagt tgataattct tttgccggaa cgactccttc aactccagcc
     1141 ccttcgattc cgacaatcac ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca
     1201 tcgccaactt ttacttcttg tacgcttttc attgcgtctt ccattgactg gttgttctct
     1261 tgattttgat caaattctgt cataacactt gtcctcctaa ccaacattct gccgtaagta
     1321 cggtactgtt tttagtttaa ttgattattc aaaaaaaata aagcgatttc gttcgttttc
     1381 cttgaaaaaa caattgtttt tcattttttt tcttaattta aaaaagaaga taaccttttt
     1441 tcaaaacaac agcttttatt gctgcaacga cttctggaat cgatttgcca gtcgtatcga
     1501 tacgaacagc atcctctgct tgcttcagcg gagagacgtc ccttgttgaa tcataatggt
     1561 ctctttcctg tatctctttc gtcagtgttt caagatcagt cggaattcct ttttcttggt
     1621 tctctttgaa acgtctttct gcacgctcac ttacgcttgc tactaaaaag attttcactt
     1681 ctgcattcgg taatacagct gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt
     1741 cccctatttt tcgttggaca gcaactaatt cttctctgac ttttccatgt gcagagactt
     1801 cagaaacagc ttttgtgaca tctggttgac ggatttctaa agtcacatct tttgatgcta
     1861 tgaatacatg ctgtccatct tcttgttgcg aaaaagagat aggatacttt ttgatcagcg
     1921 aaactagccc ttcttcatct gaaaaagata cattgttcgt aatagctaaa taagttactg
     1981 cgcgatacat ggcaccggta tctgtataaa tataattcaa ttcttttgct aatatcttgg
     2041 caacggtact tttcccagat gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga
     2101 tataactcct ttttctcaga aaaaaagccg caaagcgcgg ctattattat ttaattctta
     2161 attcttgacc agggtaaagc ataaagttgt ttggatctaa gccattcaat tcaaacaatt
     2221 gatcagtggt gatccctgca cgctcagcca cttgttttgg accttcgcct gcttgaacag
     2281 tcgttgttga accggctgca gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg
     2341 aatcccctgc ttgataagtt gcctcacttg aggaagtcgg tgttgtctct gctgcaatac
     2401 tgctttctga cgttacacct gtatcttgag acacagtaga ttcttccgcg attgtactag
     2461 attctactgt tgaagtctca gttgactcct cttttgttgt acttgtagaa ctttctacta
     2521 ctgatgtcga agtttcagaa gaagttgctg ctgttgcatt gttgcttgat ccatttctga
     2581 tccagaaata agcacctgct ggaatagcga tacataagac cagtaaaatc aaaaatagcg
     2641 aaagaaataa tgtatttcct ttcttttgtt gtctctgaga acttcttgat gaaacttcat
     2701 catctgtatc gtagattggt tgttcccatg gttcttgtgt ttcgttattt tctgtagatt
     2761 gatgtctatc ttttctgctc actgttttac ctcctaaact ctatcgtgtg tattatacca
     2821 taggaaaaag tgtcttgtcc taaaaaatca atgttccatg ttttttttaa ataaatttag
     2881 taaaatcgtt tcccaatgaa gttgttcagt cttagaaaaa agcttcctct ttggcagaag
     2941 ttctgctttt gctccatcaa tatcacaaca attctgcggc ttttcggaga gtttttcgcc
     3001 aaaataagct aagatgaatt ttctgcggca gttttcttca ttaatatagc gaagcatctg
     3061 ctgcaggcgg aattcttttt ctttttcctg acgtttgagc cgttccaccc aattttctgg
     3121 tttttcagac tgctggatca gctccatcca ttttttttgc agctcatcaa agggagcttg
     3181 ctttgtggca tattccaaat aaagctcaaa actttgtcgc tgttctctag aaagttgatt
     3241 aaagaaataa tggatacgtt catccccact ttgataaaaa agaattgctg cactttcttc
     3301 ctgatctctg cctgctcgac cgatttcttg tacatagttt tctagactgt ctggcaaatc
     3361 ataatggacc acataacgga tatcagattt atcgatcccc ataccaaatg cattcgtagc
     3421 aatgagaaac tgcagctgat tttttacaaa ctgctgttga agttgtcttc tttgagctgc
     3481 atcaagacca ccgtgatagt atccaactgt aaaacgttcc ctgaataaat gatacaatcg
     3541 ttcgacttct ttttttgttg cacaataaat gattgccgct ccttgtgctc tttccatgaa
     3601 gtgttcaagt tcttgttctt tttgctgcgt tttccgaaca aaaagcgaga tattttgccg
     3661 attgactgat tgtctgatct cttttggtgg tcgatcaaat agtacatgac gtatatctct
     3721 tgctactaaa tctgttgctg tcgccgttag tgccagtgtg accgggtttc ctaaaagctt
     3781 tcggattttt cctaactgtt gatattctgg acggaaatca actccccatt gagacacgca
     3841 atgagcctca tctactacat ataacgcaat attttgtttt ttcagatgct ctaaaagaga
     3901 aggattagtc aacatttcgg gactcaaaaa caaaaattta tattgcgata gatgacggag
     3961 gacgtatctt ctttcagatt gcgaaagtaa actattgaaa gcagctactc gtttctcccc
     4021 tcgtttttgt aattgcgtca cttgatcttc cattagagaa agcagcggtg acacgatgat
     4081 gaccagacct tccattaaat accccgtcaa ttgatagcaa aggctttttc ccgttcctgt
     4141 aggcaaaata gcgagagtat cttctccatc aagcaacgcc tgaataattt cttcttggcc
     4201 gggacgaaat gaatcaaatc caaaatattt ttttaattca tttattaaca ttctgtatcg
     4261 cttcctttct caatagatag atttgataca aacgaaattc tccataatct aattgtccat
     4321 ctacattcca ttcgctaaag cgccactctc gcacggaatc cttatttggt tccaaacgtt
     4381 cgattgtttc tgcagagaga atccactcgt atggaaaatc atcaaaaaac aaggcccact
     4441 caataagatg atctgttact gttcctcttt ttaaatgacg gattgccatg atctggtcaa
     4501 ttgtctgacc attcataaac atctgcttgg tgatcatcat gctttgattg aaattttgct
     4561 gtaataatga atcgatcaaa gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta
     4621 acaaaaggtg gatacatctg ctttgttgca gataaacctc tgactcatca ttgttttttg
     4681 ccagctgata aggaagcagc cctgttcgct catttccaga taattggtta gctaaaaaat
     4741 ctgctgcttc ttctggtatc tccgaaaaca gctgcaccaa actctcatac gcagtatcaa
     4801 tcaaaatttc acgtggcaat tggctttcac taagccattt ttttagttgg aaagtataaa
     4861 aaggacttgt ctctgctgga agatagtctt gaatacccgt tgccagatta gaaattactt
     4921 gaacagcaaa tttaatgagc cgccaactag tttcccctgt tctgccatag cggtcaaaac
     4981 gcaaaccagt atgttctatt tgatgttcgg acaacatatc agcccctgca ggcgttagct
     5041 ttgcttcatt ttcatttatt tcgatccaac cttgctgcgc aattttttgc aaaatttgat
     5101 aaaacttatc ctgcttcaat tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa
     5161 atccataaat aagtactgaa gtcgttcgtt ttcccacaag gagttggtaa agagaactaa
     5221 tccgcagctt gtgtcctgat gaaaatagcg ataaaataaa aacaatcata ttattctcct
     5281 taagttagga tggttagaaa tgtcactact atgtaaactt gtacctgaac gctgtattgc
     5341 atgcggtctt tgtcaaatcg aagcgccaga tattttcgat tatgatgaag aaggaatcgt
     5401 tttatttgcc caagaaccag aggctcatca acagttcgtg atgccggaag aagaaacata
     5461 tgtaaaaaaa gcgtatcaaa aatgtccagt tcgagcgatt ttattagaaa ataaaatata
     5521 aatataaacg aaactttaaa aacgtacact aagttctcta aacttgaaaa taagccggga
     5581 tctgtttctc ttgttccgtg aaatcccgac ttattttgaa agattcattt gctatctata
     5641 gttaattgac tagttttttc acggacccta taggacccat ttatattatt tcaacttttt
     5701 cagccagttt tccaatgtat tgacgatcac agtttgctgg tctgcgttag aaatttctgc
     5761 ctctccatct cctttttgct caccatagct gccaaaacct ccatgatttc cgccattgat
     5821 tgatacatac tctgttcttt ccggtaaata ctgcttagct tcttgataag cttcctgatt
     5881 caatacttta tcttttgtcg ccgtgatcga taaaacagga acagctgatt ctgtcaatgt
     5941 tccttttttg tcgggataac ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg
     6001 attcttattg acataacgac ttgccattac gccacctaat gaatggccac cgatcacata
     6061 atcggaattt ggaaaatctt ctttgacctt atccgctttg tttccgccca tcactgctag
     6121 atcaagtggc attttgacaa tagcgactga aaagccgttt tttgctaaat cttgcgccca
     6181 aatactataa ctttctggct ctacgagagc accaggataa aaaataagta atggagactt
     6241 tccatgattc tttttctctc cttcaaaaaa taagtaatct tctttgtttt ttccttcttt
     6301 taccaacact tgcgcttctg acgatggttc gtacgtattt tgctgtatat agtaataacc
     6361 ggctccaccg agtaatccta aaatgatgac taagccaatt agtattcgtc taaatatttt
     6421 cactttctcc caccttttct atctgagtga cccctttttc ttaatcaagt atgattatac
     6481 cttaaaaaaa taagagagaa atgcaaactt tccagcatca aataaaaaaa gacggaatat
     6541 ccatttgaat atccgccctt tttgtatgtt tatttttatt tagcacataa acgatacaca
     6601 gcttctgcat aaatttccat tgctttatac atactactta attcccatcg ttcattcgcc
     6661 tggtgcataa agtcaggagt atcagggaac atcgcaccga atgccacaca ctggttcatc
     6721 gtacgagcaa aagtagcgcc gcctgataca aacggttcag tcatgtctcc agtaatatcg
     6781 cgatatgtcc ccaatagatt tttcactaac tcactgtcta gtggcacgta taatgaatca
     6841 aggaagtcaa attcttcata agtcaaccca tatttttctg ctgttttagt taatttagct
     6901 actaaatcat ctttcttgaa tgtaacaggg attctcatat ctacaccgat ttttgtttgt
     6961 tctggtgtga tttccaaact tgcaaagttc atcgttagct cgccggattg ttcatcttct
     7021 gttttgccca cgacagcttc accagttgca ttttcttgaa ctaattttcc taagaaattg
     7081 attgggccaa aatcaaatac ttctgaaaga gcaattgcca gacgagaaat agcattcact
     7141 ccttgtgctg catctttcgc gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt
     7201 tcttcgaaat cgaattcgtg ttttttcaat gcttctttta cttcagaaag cttttctcca
     7261 gagtaaggag ctgcatcagg aacaacattc aacgctcctc cagcttttac agaaaactcg
     7321 ttcgttcctg gtcctgtcaa gtaagcttgc aataagcctt tttctgcata aattaaagga
     7381 aattcagcat ctggcgcaaa tccttgcgta attccttctt ctttttcgtt atatttttct
     7441 aaacaacgcc ataaattttc ttcatctgta ccaaagataa aacgaatacg tgtcttgaat
     7501 tccacgccag catcaatcaa agctttgacg gcatacattg ccgcaataga aggacctttg
     7561 tcatcttggg atccgcgacc aactaaccag ccgtctttta tagttggatc gaatggtttt
     7621 gtttcccaat tattttcgtc ccctgctgga acaacgtcca tatgacacaa gataccgaat
     7681 aattcatctc cagagccaat ctctgaataa ccgtaatagc cttctggatc tttaaatgtt
     7741 ctaaatccta gcttttcact gatttccaat actttatcca aagcttcaat cactttttta
     7801 ccgaagggat gtccttgtcc tgaatctgct tcatctagta cggaaggaat tcggatcaat
     7861 tcgctcaatg attctaatgc ttgttcatga tgttgttctg tcacaaattt tttcatgata
     7921 ttatcctcct aatatgttaa aaagggtcat gtcaaattcc tccgacacaa cctcttcttc
     7981 tcaaataatg acgttttaag cctgctccat cagcaagcag aatgttctta acaaatgatg
     8041 aaagaaaaaa cactcactga atggagctat tgacttgtcc acgtgcttat cgatagccgt
     8101 ttatcggttt cttcattctt atgatagacc ttgtacctgt atttgtctag gtttagaaca
     8161 ttgctgctac acctaggatc acacagatgg ctacaaataa tatcgcaatt aatttaccgg
     8221 taaatttcca ccacactgaa acatcgaaac gagcaattgc tacagcaccc attacgatag
     8281 cagatgttgg tgtaattaag tttactaaac cagatgcaga ttgataagca gtgattacta
     8341 aatccttacc gacgccggca aattcaccca taggtcccat gattcccatt gttgcagcag
     8401 ctaagccgga agttgaagga atcaagaatg acatagggat atagaaaata aatgtaataa
     8461 tgatgaatac acttgaagat aatgaactta atccttgttc gccccaatgt aagatggtgt
     8521 ctgtgattaa tccattgttc ataaccactt ggataccacg agcaacagct actacgattg
     8581 ctacaccgat caaatcagaa gcaccagcta aaaattcaga gacaaattca gactctttca
     8641 tgccataagt gattgctact acgatggaca ttactaagaa taacatagta atttctggga
     8701 agtaccagtc acctaatgga agcatgcttt tacctagaac cgtacctagt accggaaggt
     8761 ttgttaacca gtttgtcaat gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag
     8821 gaatcaaact gatgatcatg ataccaaaag taataaagaa caatacatta acatgttttt
     8881 gttttttcgt gattacttct tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt
     8941 ctttacgttg gttgtatact aatgattttg ttggatcttt ttcgatcttt gatgcataac
     9001 gatacacata tacaatagaa atagctaaca aaataacgaa catcaagaat cgaagaccaa
     9061 taccttcacc catcgaaatc ccaacagctt gtgaagctac gcctgttgca aaaggattta
     9121 ccgttgaagc tagacagcct acttgagacc ctactaatac gattgctaca gctactactg
     9181 tatcaaagcc tacagccatc atgactggga tcagtaaagc atagaatgga atcgtttctt
     9241 ctgccattcc gtaagtagag ccgcctaatg caaataacag catcaatact ggaataagca
     9301 ttttttcttt tcctttgaat ttcttcacaa ctgaagcaat cccggcatct agtgcccctg
     9361 ttttgttgat aatacctaaa aatccaccaa caaccaaaat gaagaaagag attgggattg
     9421 cagcaggtgt ttctttagtt agctcccccg ctggtacacc tagcatccca ttgattggcg
     9481 ccatgaaaat atcccacagc ccttgaggat tttgttgtac tcgttcatat gatccagcga
     9541 cgatgttgcc tgcatcgtct acactatagt gcccagctgg tataaaccaa gtaagtacag
     9601 cgattactgc aataataata aataagaccg tatacgctga aggcatttga aattttttct
     9661 tttttgtttg ttccatcttc ttcttccttt cttttccgtt agagacaaca attttggctc
     9721 taatgttatt ttatcaaaaa gagtatcaga acaaaaataa taacacgcat tttgtaaaac
     9781 ttttgttcaa aaagttcgtt tttccctatt ccctcctctt ttttcagttt tttaaagctt
     9841 tcttcttttt cattgtagcg tttccttttt tttataaaca aacattttcg tcttttaaaa
     9901 ggagaagaat ttcacatttg caaagaattg attgattttt atgcataaat caaaaaaata
     9961 ctcaaaacaa acaaataaaa cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa
    10021 aaagaccaag aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc
    10081 gaactgatgt tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac
    10141 agcactcacg atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt
    10201 tgctaaagac atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg
    10261 cgtaataacg aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat
    10321 gacacctaaa aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat
    10381 cgggaatgta aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg
    10441 agaaaaacct gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc
    10501 cgctggaccg aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa
    10561 aaaactaaaa gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc
    10621 aaacatcgct actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt
    10681 taggactcct cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc
    10741 cagggaaaat agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt
    10801 atccagacta tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag
    10861 gtcgtggact ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat
    10921 aatttttgtt tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacaatta
    10981 taaaaagtaa gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt
    11041 tcacggtaat ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt
    11101 agtttttgca caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct
    11161 tcgtggatcg tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg
    11221 aatttagctg gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc
    11281 atcgattttg tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt
    11341 ggatgtgtca ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta
    11401 tcgtaatcca gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta
    11461 acaacttttc ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat
    11521 aaatagtaac cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt
    11581 ttcccaactt ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca
    11641 aggatgaatt cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc
    11701 aatctctcca ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca
    11761 cgtcttcttc aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca
    11821 aaccaaaata atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt
    11881 ctactctacc tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa
    11941 cctgaactcc tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga
    12001 ttgctaaggt ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct
    12061 taagtaaggg agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa
    12121 tatgcaaagc tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg
    12181 tgacttctgc ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct
    12241 cgccaacaat aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct
    12301 actgctggat aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct
    12361 tttttcatca attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac
    12421 tcaaaaaaag aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat
    12481 accattcgtt cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa
    12541 aatttcttac gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt
    12601 cctcgcttca acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg
    12661 taataaagac ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta
    12721 cggtattcta aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca
    12781 tcgtctgtgc cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta
    12841 gcagccatca ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata
    12901 taattcatat attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt
    12961 tgctgaatca gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg
    13021 atctccgtca actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag
    13081 gctctgtgat ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta
    13141 acatttgaaa gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga
    13201 tcagctgatc gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt
    13261 ctggcgtaaa gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca
    13321 acaaagaagt tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac
    13381 ggttttcttt ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa
    13441 aatgctgctt ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg
    13501 tagagatatc cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta
    13561 gcagatgtgt ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag
    13621 cctctcgtac taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat
    13681 gattattgac aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt
    13741 ctaagtagcg ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat
    13801 ctccttttcc aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc
    13861 caatcaattc actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc
    13921 ttatccctaa cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag
    13981 tcaatgtact tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat
    14041 gatcagtgat acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc
    14101 gagtaacagt tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta
    14161 aaaagcgatc gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact
    14221 gttccaaatc acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca
    14281 agtagtggag ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc
    14341 atatcagata ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg
    14401 tctagacact cagacggact ggtttctttt tt
//
LOCUS       DAXWLG010000062        14317 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXWLG010000062 DAXWLG010000000
VERSION     DAXWLG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.062"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            45..1385
                     /locus_tag="ACGE4T_001462"
     CDS             45..1385
                     /locus_tag="ACGE4T_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8386743.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1603..2046
                     /locus_tag="ACGE4T_001463"
     CDS             1603..2046
                     /locus_tag="ACGE4T_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFF8386744.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2550..3968)
                     /gene="nhaC"
                     /locus_tag="ACGE4T_001464"
     CDS             complement(2550..3968)
                     /gene="nhaC"
                     /locus_tag="ACGE4T_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8386745.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4371..5594)
                     /locus_tag="ACGE4T_001465"
     CDS             complement(4371..5594)
                     /locus_tag="ACGE4T_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8386746.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5625..6464)
                     /locus_tag="ACGE4T_001466"
     CDS             complement(5625..6464)
                     /locus_tag="ACGE4T_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8386747.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6481..7161)
                     /locus_tag="ACGE4T_001467"
     CDS             complement(6481..7161)
                     /locus_tag="ACGE4T_001467"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8386748.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7163..8236)
                     /locus_tag="ACGE4T_001468"
     CDS             complement(7163..8236)
                     /locus_tag="ACGE4T_001468"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8386749.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8292..8530)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8573..8806)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8883..9554)
                     /locus_tag="ACGE4T_001469"
     CDS             complement(8883..9554)
                     /locus_tag="ACGE4T_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8386750.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9641..12289)
                     /gene="mgtA"
                     /locus_tag="ACGE4T_001470"
     CDS             complement(9641..12289)
                     /gene="mgtA"
                     /locus_tag="ACGE4T_001470"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFF8386751.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12631..12805)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12978..13415
                     /gene="ndk"
                     /locus_tag="ACGE4T_001471"
     CDS             12978..13415
                     /gene="ndk"
                     /locus_tag="ACGE4T_001471"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFF8386752.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13437..14303)
                     /locus_tag="ACGE4T_001472"
     CDS             complement(13437..14303)
                     /locus_tag="ACGE4T_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8386753.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
BASE COUNT         4096 a         2997 c         2538 g         4686 t
ORIGIN      
        1 ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat
       61 tgaaaaaaca acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg
      121 caggtctatt taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg
      181 cttttttgat tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt
      241 taagcggtgg cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc
      301 attctgctga ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg
      361 aagctgttgc agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat
      421 ttgtttttat gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg
      481 cagaaacaga atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt
      541 ttgctactta tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc
      601 aattgactga ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc
      661 ttgtagttat ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag
      721 atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct
      781 tttacatcat tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca
      841 cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata
      901 tcgtcaattt tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat
      961 cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg
     1021 ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc
     1081 ttggtgtagc tctttcttat tttgtcggtg acaaattatt tggctatctg gcaggttccc
     1141 tttcgtatac agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac
     1201 taagaaaagg aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat
     1261 tgattttcct tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc
     1321 tttacctgtt catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg
     1381 aatgaaatcg atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa
     1441 ggagttgtga caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc
     1501 ttggtaaacg gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg
     1561 acatgataga agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag
     1621 tttttgttga tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc
     1681 tatgcgctcg atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt
     1741 gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata
     1801 aatgttatga tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga
     1861 agccaaacta ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc
     1921 gctatcctgg caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa
     1981 gaaatcattc aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg
     2041 gaatgaccat tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag
     2101 aaataagaag aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg
     2161 catcaagtaa taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc
     2221 ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt
     2281 ctgacctgag tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg
     2341 agctaatacc gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc
     2401 ttagtttcgc gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt
     2461 ggcttaatac cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa
     2521 aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact
     2581 gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc
     2641 gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata
     2701 ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta gttgattact
     2761 gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa
     2821 gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc aataaaaata
     2881 tttactccga tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt
     2941 cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat
     3001 gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta
     3061 agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca
     3121 gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg
     3181 atattcaaca aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa
     3241 aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact
     3301 tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag
     3361 aaagaaacaa caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa
     3421 tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct
     3481 ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt
     3541 gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca
     3601 gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc
     3661 aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc
     3721 aatgaaccga ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg
     3781 attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt
     3841 ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca
     3901 atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt
     3961 tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc
     4021 ggattactcc gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt
     4081 taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac
     4141 tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg
     4201 gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg
     4261 acttgtacga agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc
     4321 atttttctga aaattatttt tataactttc acacacaagt ttatttttta ctagaaagta
     4381 gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat
     4441 agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca
     4501 atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca
     4561 atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt
     4621 cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca
     4681 cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca
     4741 tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct
     4801 cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt
     4861 aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta
     4921 atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta
     4981 aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg
     5041 gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc
     5101 ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct
     5161 cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat
     5221 acacggattt ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg
     5281 gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat
     5341 tctgccaaaa aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct
     5401 gttcggtgtc cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc
     5461 aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga
     5521 gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag
     5581 attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat
     5641 gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct
     5701 tctgtttgat aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt
     5761 gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca
     5821 tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct
     5881 acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt
     5941 ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc
     6001 aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt
     6061 gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc
     6121 agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa
     6181 ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc
     6241 acatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc
     6301 aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg
     6361 atagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata
     6421 attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt
     6481 tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat
     6541 tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga
     6601 tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat
     6661 cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata
     6721 cacacgtaaa atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc
     6781 aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa
     6841 aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt
     6901 caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt
     6961 atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa
     7021 cgtcacacct aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata
     7081 tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata
     7141 tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta
     7201 aatcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt
     7261 ttttccgctg tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt
     7321 gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat
     7381 gagcgattag aggttcgttc gtctgatttc caatataact taattccacc agccatttat
     7441 tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat
     7501 gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga
     7561 tgctgctacc tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt
     7621 ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca
     7681 atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt
     7741 catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt
     7801 tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt
     7861 ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag
     7921 aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca
     7981 taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct
     8041 gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg
     8101 tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta
     8161 aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat
     8221 ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa
     8281 aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc
     8341 acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat
     8401 cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt
     8461 gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca
     8521 atgcaaataa tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga
     8581 cgacgtatcg cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa
     8641 aaagagagat tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac
     8701 aaattattct ccatggccat tttcagcttt tcttacttga ctcttctcac cagccagagc
     8761 ctctctgcaa agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg
     8821 gattaatcta catgaggaaa actgaaaagt caagtacgaa attcaaatta ttttttctat
     8881 atttattttt ctgttttttt atttaacatt tcaataacag ttcgaagatc ttttccttcc
     8941 gcaagaagtt tcaatttttc cttttcttcg acggtataat ttcggacaaa actggaatca
     9001 ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg ttgctcctac taaagaatct
     9061 tccactgcaa aggctgaact agcttctaat cctgttcgtt ccaaagctgt cagataagga
     9121 atcggtgaag gcttgttctc cgacacttcg tctccgaatg taaacgtgtc caagtaatca
     9181 gctaatttaa agtgatccaa gatatctgtt gctcttttcg taacagtggt cgtagcaatg
     9241 cctagtttca tatgacgttc ttttaagtct gccaatattt cttttgcata tggttttaag
     9301 tggatggctc cgcttgtcag ctgttgataa aaatattctt cacgcttcgc tcggacttcc
     9361 tgtacttttt cagggctacc tgctgctttc actaaaatat caaaactttg tttccagttt
     9421 tttccgcccc aagcgtcaac gatttctttt gggatatgga tatcaaattg ctgaaatccc
     9481 caaagccagc catcacgata cgttttttct gtgtcaacta acaaaccatc taaatcaaat
     9541 attgcacctt tcatctatac attaatcctt tccaaaacaa tacacatagt ttagcacgac
     9601 tggtccaaaa agaacagaag caaagaacca gccttctcgt ttataagagt tcttggaatc
     9661 gacggatata gattttcttc acagctgtca caagcgccag ataagcgatg actgtagcaa
     9721 ataaccataa ccagtaattg ccaggaagtg gcatcaagcc taattcagca ccaaaacttg
     9781 taaaaggaag gacagaaccg acaataattc ctaatgtagt gataacaaac atggcaaatg
     9841 ttgcgttgct ttggataaat ggtactttcg gtgtacgcag tgcatggagc actaaggttt
     9901 gtgtccatag tgattcgaca aaccatcctg catggaataa tgcgatgaaa atcactcgct
     9961 gtgaactatc caatgtatgg aaacttccgc ccactacagc aggacaaata acaaaataca
    10021 tcaacaagta agtagtaata tcaaatatcg aacttgttgg accaaagtaa accatgaatt
    10081 ccccaatact agatgcattc catttttttg gctcgtccaa atattcttta tccatccgat
    10141 cccatggcaa ggaaatgcag gaaacatcat agatcagatt caaaaacaaa agttgtaaag
    10201 gcagcattgg taaaaatggc aggaacgtac ttgcaatcag cacggaaaac atattgccaa
    10261 aattagaact tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt tctcgtccag
    10321 aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgcagatt
    10381 ctttcgcaat atccacagct gtatctacag aaataccgat atcagccact ttcattgcag
    10441 gagcgtcatt gataccatct cctaaaaatc cgacagtatg accattttta cgtaaagctg
    10501 tcgtcaaacg tgctttttgc tgaggattca attttacgaa tacttcatgg tcttcagcta
    10561 cttgtgctaa ttcatgatca ctcatttccg agattttctc accggtaacc aattcattga
    10621 tctctagacc tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa
    10681 cttttacagc tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg
    10741 gtggatcaag gaaagcaaga taaccaatca aaaccatgtc tgactcatct ttgactgaaa
    10801 attctccaac gacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccatctt
    10861 cattaagtga ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa
    10921 cagtaccgcc aagatcaata aatttagaaa tgttcagcat ttcttcaatc gcgccttttg
    10981 tgatcatttg tgttttacca gctggatcag caaccacaac actcatacga cgacgttcaa
    11041 aatcaaaagg aatttcatcc actttttgat aattgatctt atctgtatct aaagtttctg
    11101 ttgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctat
    11161 ttagatacgc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt
    11221 caagaatgat tttatcttgt gtcaatgtcc ccgttttatc cgtacataac acatcgattg
    11281 caccaaagtt ctggatggca ttcaaatttt tgatcactgt tccttttttg gccatcacac
    11341 ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggt gttagaccaa
    11401 ctgctacaga tagtccaaaa agaaaagctt ctagccaatc cccttttgta agcccattga
    11461 tgacgataac tgctggagcc atcaatgaca taaatttgat cagtagcatg gatgttttat
    11521 ggatgcctaa ttcaaagctt gattgaattg gtttttcaga tagcgtttct gctacatgac
    11581 caaatagtgt ttcatttcca acagaaacaa tgattccttc cgcacttcca ctgatgacat
    11641 tacttcccat aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata
    11701 ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata
    11761 aatcctttgc ggacaataaa cgcatatccg ctggtatcat atcccctgct gaaagccgaa
    11821 caagatctcc acagacgatt tcttccatag gaatctctgt gtactttcca ttacgtttta
    11881 cagctgccgt tactttcaca agtgatttca gtttttcagc cgcttgattg gaacgtacgg
    11941 actgaatcaa cgtcattgtc ccgctgacga taaccatagt gaagataatg gctacaccga
    12001 aaagatcttt atctccagga gctgctaata catattccgt gatgaatgaa ataattccta
    12061 atgcgatcaa cactaatgta aatggcgtga tgtatgcttt caatatttct acaatgaatg
    12121 gtgttttttt gccatatgaa atcgcatttt caccgtaatt ttctcttgaa cgttcgattt
    12181 gttcttctgt caatccattt gctgatgcat ggaatagttc aaataattct tgtttcgttt
    12241 ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc tcaatctctc
    12301 ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc tttagctatt catcggatcg
    12361 atagagaaaa aactgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta
    12421 gatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt
    12481 taattcatga cttccaatca tttttcgaat agttattttt ccaattagac cacctccttt
    12541 ttatttttca ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga
    12601 aaggtttgaa aaagcatatt aaaaaaagct tctcagatct catcgttgag ttttgacact
    12661 gtacaacgta aaaagcagat acctgctttt agctacctta tgaaaagcct tatctcgaca
    12721 attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata
    12781 ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga
    12841 ggatgtcaaa tcaattttcc atgtttttca tacttttttt aaaatacaca cgttatattc
    12901 ctgtctcacg cggttttatt tgtctcattt tccacctccc gctataatag aaatatagta
    12961 ggaaacgagg tgctgttatg gaaagaacac ttgttattat caagccagac ggcgtgagac
    13021 gccatttagt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa
    13081 tgaagtttga aactatgact cctgaattag caaaagaaca ttatcagcac ttgactgagc
    13141 gcagtttttt tgatgaactg atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc
    13201 tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca aaagctgccg
    13261 atgctgttcc aggtacgatc cgaggagact acgcgctgcc ggggactgaa aacatcattc
    13321 acgcttctga ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc ccgccattcg
    13381 atataaacaa accgtctgaa aaagcggcga agtgatacaa attctaaact gctggtctag
    13441 ttgctgggtt cagcaatggc cagcagtttt ttctctaagg acctccgttg attctttcct
    13501 aacatatatt cgagcactgt gaagaattct tggtaatctt tcgtcttttc ttcccctaaa
    13561 gcagcagaaa tcaatctttg ataataatct gccatcaatg tttcatagat cactgtactt
    13621 ctttctttat gtgctaagct ccgtttcata tcttcattca cactacgtgc ctttttcaac
    13681 cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag atactgctca
    13741 tgattgttca actgtgctaa tcgtcgcatt ctttttttga ttttcaaata attgttctca
    13801 atgaaccgat cactgaatat ccaaggaaaa tcagctagaa tagatagttc aaattgacca
    13861 tagtctggta tctgcatcaa gtattcttca atgattcggc attcataacg taaaaattca
    13921 aattctgtct cagatacttc tgttttttcg accatgtgaa gattcaaaac caaataatac
    13981 agatcttctg ttttcttaaa atgcctacgg ttattgctga tctctttata aggatcaaag
    14041 ctgtcttctt gtacccgatc tagcattcgt ctttttttct ttaactttat tggttcatcc
    14101 agctggtttc gcatccattc atattctgca agtgtcatgt tcattcgctc taaaaatgat
    14161 ctcaatgtta caaatgacat ttttttcact gaatttgtct caatcccgac tagagaagtc
    14221 ttaaaacaga ttccttccgc taattgggac tgtgtcaact tcttgttgac tcgaagtttc
    14281 ttgatcaatt caccatcata cataatagtt ctccttt
//
LOCUS       DAXWLG010000063        13661 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXWLG010000063 DAXWLG010000000
VERSION     DAXWLG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.063"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(3..1304)
                     /locus_tag="ACGE4T_001473"
     CDS             complement(3..1304)
                     /locus_tag="ACGE4T_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8386754.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(1534..3525)
                     /locus_tag="ACGE4T_001474"
     CDS             complement(1534..3525)
                     /locus_tag="ACGE4T_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFF8386755.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(3669..3935)
                     /locus_tag="ACGE4T_001475"
     CDS             complement(3669..3935)
                     /locus_tag="ACGE4T_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8386756.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE4T_001476"
     CDS             complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE4T_001476"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFF8386757.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE4T_001477"
     CDS             complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE4T_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFF8386758.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            7279..7842
                     /gene="def"
                     /locus_tag="ACGE4T_001478"
     CDS             7279..7842
                     /gene="def"
                     /locus_tag="ACGE4T_001478"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8386759.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(8095..8616)
                     /locus_tag="ACGE4T_001479"
     CDS             complement(8095..8616)
                     /locus_tag="ACGE4T_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFF8386760.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(8619..9461)
                     /locus_tag="ACGE4T_001480"
     CDS             complement(8619..9461)
                     /locus_tag="ACGE4T_001480"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFF8386761.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(9454..10179)
                     /locus_tag="ACGE4T_001481"
     CDS             complement(9454..10179)
                     /locus_tag="ACGE4T_001481"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFF8386762.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            10355..10651
                     /locus_tag="ACGE4T_001482"
     CDS             10355..10651
                     /locus_tag="ACGE4T_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386763.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      10907..11081
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            11219..12811
                     /locus_tag="ACGE4T_001483"
     CDS             11219..12811
                     /locus_tag="ACGE4T_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8386764.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            12824..13564
                     /locus_tag="ACGE4T_001484"
     CDS             12824..13564
                     /locus_tag="ACGE4T_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8386765.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         3923 a         2846 c         2604 g         4288 t
ORIGIN      
        1 ttttattcta tttttctttc cgtggactct actttgaaat aatcgatatg tgcatgctgg
       61 tattctaata ccgtgaaacg gaatgatgca atctcaatga ttgttcctgc ttttatttct
      121 ttatatcggc tagaagcaaa gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga
      181 atctctacat gaaaataacg ctggaaatca tctaatgtta gtttgccgga gatgatataa
      241 gagccatctg actgttccgc tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc
      301 ccgacgattt cttctaaaat atcttcaatc gtcactaagc cgcttgtccc tccgtattca
      361 tcttttaaga tagcaatatg ctttcccttt tttcgcatga tcgtcaataa tttttttata
      421 gggatcgttt cagttgttac gatcggctct tcaatcaact gttctactgt cagctggtca
      481 tctttataag attgcttgat caaatctggc aaggtgacat aacctaaaac attgtctttt
      541 gattctaaga taaccggata gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct
      601 ttgacggttt ctgaagcttc gaatacttgc atggacgtac ggtctacttg tatatctttt
      661 gccaaggtat catcaaattc aaacacgttt tccaaataat gatattcttc tttggtcaac
      721 tctccttttt cataagaatt caatgcgact aatgtcagct cttcttgaga caatgtctct
      781 tctccttcag atacgagacg cacacctaat agcttcccta gccctgcagc tgaatgattc
      841 aatacccaaa taaaaggaaa cattacttta taaaaataat gaagtggttt aactacgaac
      901 aagacgacct tctccgtatt gacgatacta tagctcttag gaagcagttc gccaataacc
      961 acatcgatat acgtaactaa aataaatccc agcgccacag agattgtact gctaatagcc
     1021 gaactaaaag gtagtttacc gatcactggt tctagtaaag ccgcgacaga tccttcaccg
     1081 acccacccga tgatcaaacc ggttagcgta attcctaatt gactagctga taaatagtta
     1141 tctaaatggt gtgtaacatg aagagctagc ttggcgttct ttacattttc cttttctagt
     1201 tgttccaatc ttgatcgtct gattttcact aaagcaaatt cacttgctac aaaaaatgaa
     1261 gtaattgcaa tcatcaaaaa taaaataatg aaattgataa ccatattctc ccgcctttct
     1321 ctttttctat tgtacccttt ttctacaaaa agattgaaaa taatgcgtat tcatatttag
     1381 gaataaaaaa ggagatgcgg catttttcag ccacatctcc ttatcacttc aaaatcattg
     1441 cgttacttgt tgctaaacag ctttttcatg acctctgata aacggtgttc aagtagctga
     1501 cattcgacat tagttcaggt caaagcgcta ctttcacaac cgctctttca accatgtttt
     1561 ccgagtattt ccaatgataa gcggaacagt ttcatgtttt acttcgctgt actctagccc
     1621 aaaccatgtt tctggcgagg tagcgatttt tttgattgcc agttttgcct gcatgatttg
     1681 acgttgccat tttcctagag ttgtcacatt ggaaactact tcatcaatca ggacaaattg
     1741 gaaatcgcca acttctcgcc ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg
     1801 gccttgtttc atcaaatcat ggatgacttg gcgaacatat acgttgattt cctgttggac
     1861 cctgaaacct aagaacagat tcacttggac aataaaatca gtgtccatca tatctacttg
     1921 atattctttc gtatatggct catccgtcac ttccacatga acaaaccaat agactcgtgc
     1981 tcttttaggc cgtttatcta aaatcgaata gatgaactgt cggccaactt tatcttcagc
     2041 catatcagga accaagaaga ccacatttgt ctgatagatt ggtaaagaag tatcatcacg
     2101 taattctcta agctgcggga tataatctct caaagacaca tcctcggttg acgattcgcg
     2161 aataatgttc ccttgttccc aaataaacat cacgactaag atgatcaaac caatcagaac
     2221 ggctacaaaa ccgcctcgga agaatttgac aacacttgag ataaagaaaa cgacttctat
     2281 cgcaccgaag aaaaaggtaa ctaaatatgc aagccaacga gaatgcccat tttggataag
     2341 ataaaagtaa agcagtgctg ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc
     2401 agcttccata tgatgagacg ttcggaatgt gacaaccact agagaacagg cgatccataa
     2461 aatcagattg actgctggga tatacatttg cccgatactg ttcccaggat acatgatctt
     2521 taaccgaggc agcagtttca acttgattgc ttctgaaact agcgtaaacg aacctgtaat
     2581 caatgcttgc gaagcaatga ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca
     2641 actttgaggc atcatttgga agaatggatt caggttcttg atttgctgtg cttcagggtt
     2701 ttgataaaca ttcaacaacc acgcagcctg cccaaaataa ttgataacca aacaaatttt
     2761 gacgtaaggc cagctcagac gaatatttcg tttgccgaca tgtccaagat cggaataaag
     2821 cgcctctgca cctgtcgtag ccaggaaaat atttccaaga atgaacaagc ctaatttgtt
     2881 atctgcacta aaaagcagct ggatagcata ataaggattc agtgcacgga taactgtcca
     2941 gtcttgactg aagttcatta atcccattac gcctaaaaaa gtaaaccaag cgaacatgat
     3001 tggtccgaat gcttttccga caatatctgt accaaagcgt tggaccatga ataaaacaaa
     3061 aataataact aaagtgataa tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa
     3121 ctgcggaatt cctcgcaagc cttcaatcgc agtcgttacc gtcacagcag gggtcaatac
     3181 cccatctgct agtaaggcag ctcctccgat cattgcagga atgatcaaat atttcccacc
     3241 ttttcggaca agtgtgtaga gtgagaaaat tccaccttca ccatggttat ctgcattcaa
     3301 tgcaatcacg acatatttga ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac
     3361 tgatccgata acaaaatcag gattgattcc tgacaatccg ccattatctt caacaatcga
     3421 tttcataacg taaagcggac ttgtcccgat gtctccgtag acgactccca tcgctacaag
     3481 caggccagcc attgatatct ttttggtatt gtgcgttgtt gccacgtttt tccctcattt
     3541 cttgttatat ggatatcctt taggtaagct tgaatgatag atgatattct tatccatgac
     3601 acccgctttc ctaaattgct tctaattatc caagtaacac actgaagtgt caacttattt
     3661 gaataaaatt attctttctt tggacgttct ctgatacgtc ttgccacaaa tgaataaaga
     3721 gcaaggcaaa aaattgcacc caataatgct ggtatcaaat aaaaaccgcc gagttccggt
     3781 cccagtggtc ctagtaaaaa ttcgccgagc caactaccta aaaaaccgat aatgatattt
     3841 ccagatacgc ctcccgggac ttcctttcct aaaattgcgc cagctatcaa acctaatata
     3901 ccgccgataa ataacgacca taaaaaattc agcatctcct ttccctcctt tatccatatt
     3961 tctcactttt attttacgct ttattcagaa ggactccaaa aatatgcgcc aagaaactcc
     4021 agaaaaaaag aagttgtaac aaaagtgttc agcttatttc cgtagaagct gcttctggaa
     4081 caccgtgaat cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg
     4141 aaaaatagtt cctcatattt tgccgttttt tgagctaata tcgagggaca gctgattgaa
     4201 catcgtttat tggagaggtt gtaaaaaagc tgttctgcat caagtaataa gaacagccaa
     4261 gtaaaaacag ctcctaatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt
     4321 tgaacaccgt ttattcgaaa aaaaaggact gtgacacggc ttttgtcaca gtcccctttt
     4381 ttcgaataat tattgtcccc ctgtcacctt cacgatcaca agtacattat tcctatgaat
     4441 taaattattt ttcttcattt ttcgttgaac catcttcgtt caacattgct tttcttgaca
     4501 gatttacgcg tccttgttta tcgatttcag ttactttcac aaggatctca tcacctagtt
     4561 taacaacatc ttcgacttta ttgacacgat cgtttgctaa ttgggaaata tggacaagtc
     4621 catcttttcc tttgatcaag ttgacgaatg caccgaattt ttcgatacgg acaactttcc
     4681 ctagataaac ttcaccaact ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt
     4741 ttttgatcat ttctgcatca gctgaagcaa tacttacttt tccatcttga tcgatatcga
     4801 ttttcacacc agtttcatcg atgattccgt tgatcgtatc gccgccttta ccgataacat
     4861 ctttgatttt agcaggttcg atctggatca tttcgatttt tggtgcatat tggcttaatt
     4921 cttcacgtgg agcagcaatc gttgaagtca attcttctaa gatttccata cgtgcttgtt
     4981 ttgcttgagt taatgcttca gttaggattt gttctgtgat tccttgaatc ttgatatcca
     5041 tctgtaaagc agtgatacca tcttttgtac cggcaacttt aaagtccata tcgccaagat
     5101 gatcttccag cccttggata tcagttagga tcgtatagtt ttcaccatca gaaacaagtc
     5161 ccatcgcaat accggcgact ggcgctttga ttggtacacc tgcatccatc aatgctaatg
     5221 tgccagcaca gatacttgct tgggaagaag aaccatttga ttccaatact tctgctacta
     5281 aacggatcgt ataaggaaat tcttcttctg aagggattac ttgtgctaac gcacgttcac
     5341 ctaatgcgcc atgtccgatt tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa
     5401 cagaaaattg agggaaattg tagtggtgga tgaatcgttt gctgacttcc acccctaaac
     5461 catcaatgat ttggtgctct cctagaggtg cgagtgtaca agctgataaa gcttgtgttt
     5521 gtcctcgtgt gaacaaacct gatccatgta cacgtggcaa taagctgacc tctgatgaca
     5581 atggacggat ttcatccaat ttacgcccgt caggacggat cttatcgatg gtgatcaatt
     5641 cacgtacgac atctttttca agatcttctg tgatttgttt aacttctttc aacaagtgat
     5701 cttcatcttc atgacctgca aatttctcag cataagcttc gcggatatcg atcttcacct
     5761 gctcgatatt atcttcgcga gcaagttttt cttcagtcat tactgctgct ttcattgtag
     5821 cgtaagaagc atcaaagatt tcttttttca atacttcatc aacttgcagt aaagtcactt
     5881 ccattttttc tttccctact gcttgaacga tttcttcttg gaaagcaact aactctttga
     5941 tagcgtcaaa accaaacagc aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc
     6001 cgctttcaac catattgatg gcttgtttcg ttccggcaac agtcaattcg atatctgttt
     6061 gttctgcttg ttctactgtt ggattcaaga cgtactcgcc atttacacga ccaacttcta
     6121 cgcctgcgat tgggccgtca aatgggatat ctgagattga tagtgctaaa gaagaaccta
     6181 gcattgctgc catagctggc gagcagtctg tttctacact catcacgatg ttcgttactt
     6241 ggacttcatt acggaagcct tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg
     6301 cagtcaatgt tgcttccgta cttggacggc cttctcgttt gatgaaacct ccagggattt
     6361 tccctgctgc atacattttt tcttcataat tgatcgttaa tgggaaaaag tctgtatctt
     6421 ttgcttcttt agaggccaca gctgcactta agactactgt atctccataa cggactaaaa
     6481 cggctccgtt tgcttgtttt gctagctgac cgacttcgat ttgtaaggga cggccgcccc
     6541 atgtcgtttt gaatacttgt ttttctgaca taacttctcc ttttccttct cgtgaaagca
     6601 agacgctact aaacaaatga aaactgccga aagccgtctt ttgagcagct ttcatttggt
     6661 tagtaggagt ccatctagct tgatcacgga attttactta attgatatac gagtaaaaaa
     6721 agtgagattc atgtgaatcc cactcttttt gactaaaatt aacgacgcaa gcctaaacgt
     6781 ttgatcaatt cgcggtaacg ttgaacgtca gttttacgta agtaagctaa caagttacgg
     6841 cggtgaccaa cttttttcat taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt
     6901 gcatgttcat tcaagtggtt gatttcttca gttaatacag cgatttgtac ttctggagaa
     6961 ccagtatccc cttcatggcg agcgtattcg ttgatgattt cattcttgcg ttcttttgaa
     7021 attgccattt ctttcacctc tttcgattat tcccctgcga ctgagtaaaa cgttggtgat
     7081 tcgcttaacc aagtggcagg tcatgtgcct agagcacaca gctatacttt acctgaaacc
     7141 gggttgaaaa tcaagtagtt aatcattaag aaaaggtagt tttcaaacaa tcttactact
     7201 acttttccat ttttgtggta aactataatg cgaaaataga cttgaaacac agtcttttaa
     7261 gaaaggggta catattccat gattacaatg gatgatatca ttcgtgaagg aaatccgacc
     7321 ttacgcgaag ttgcaaaaga agtttctctt ccattaagcg aagaagatat ctctttagga
     7381 aaagagatgc ttgagttctt aaaaaacagc caagatccaa tcaaagctga agaactacac
     7441 ttacgaggcg gtgttggctt agctgctcct caattagata tttctaaaag aatcattgct
     7501 gttcacgtac caagtcctga cccagaggca gatggacctt ctctaagtac tgttatgtat
     7561 aatcctaaaa ttttgagcca ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt
     7621 ttatctgttg atcgagaagt accaggatat gttgttcgcc atgccaaaat cactgtgtct
     7681 tattatgata tgaatggtga aaaacacaag attcgtttga agaattatga atcgatcgtc
     7741 gttcaacatg aaatcgacca tatcaacggt gtgatgttct atgaccacat caacgatcaa
     7801 aatccatttg cactaaaaga aggcgtattg gtgatcgagt agagaattgg caggttcctt
     7861 cgatactatg ctacaagaaa gctttataaa aaaggggagt gctcaaaagc tgcacttccc
     7921 ttttttatgt ttctccacta atcgaaattt agatagacat ctagaaatcg caattccaaa
     7981 aaattctgtg tattggatac gcatcttttc ttattttttc ttatttcttc catatgggtg
     8041 tttatctcag agatgataga atagtttcta ggataaaaag tagcagctac gatgttatcc
     8101 ttcgtagagg attcctgctt cagctagttt ttgtttgatg tttttcagca tatgcttttc
     8161 tgggtagcgg atcgtatgca gatggatgcc gcctgttaat tcggataaca aagtagcatt
     8221 acttttttga taacgtctca taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt
     8281 tcctgctaac tcaccgtaaa ggggatgttc cacaattaca tcaatgattt caccaccatt
     8341 ttcaacgatt agtctcagtt cttcttctgt ttgatctttt ccatgctgga cagcgatttt
     8401 actgatgtgc ccagtattgg tttcttctgt ttcgaggata taacctcttg ctgttgccac
     8461 gatactttta cctgcagctc gtagtaaagc aatatcgcct acaatgatct gacggctgac
     8521 tccaaaagtt ttcgcaaact tgctagcact cactggtttt tctgtatttg ccaaggtttg
     8581 catgatttgt tttcgccgtg tctctccttc gatcacactt attcctcctt cattttgttt
     8641 tggtattcgc gcgagtgttt catcaaatca acatacgtat ctgttattgt ttccttatat
     8701 cccttatttt tcagcagact aacaacttta tccaatactt gctgctctct ttttgcatca
     8761 taaactgctt ggttttcttc ttttttcact tgtcctattt tagttaccgt atccattctt
     8821 ttttctaata aagcaaccaa ctctcgatcg atcatatcga tcttatgacg taatgtttca
     8881 agagactctt ctgttagttc ttcctctact gccttgatga tacgagcagg atttcctgca
     8941 atcactacat tgtcgggaaa agatttggtc acaaccgaac ctgcgccgac tactacgttg
     9001 tcccctagag tgacacccgg cgtgacgata actcctccgc ctaaccaaac attattaccg
     9061 attttaatgg gttttgcgta ctccagaccg ctgttacgtt tcactggatg tagtggatgc
     9121 gtcgccgtgt aaagctggac attcggagcg aacatgcagt tatcaccgat ttcaatcgtg
     9181 ctgacatcta aaaatgtaca attgaaattt gcataaaaat tctccccaac aaatatatta
     9241 tatccgtaat caaaattgat aactggctcc atatagattt ttttccctgt acggccaaaa
     9301 gtctctttca acaattggga tcgcagttcc gctgattctg cttggttgat gatttggctt
     9361 tgacttcttg caaacatacg gtcggcaacc agctcaggat cattggcaaa ataaagctcg
     9421 cctgcaatca ttttttcttt ttcggttttg aattcagtca taatctgctc ctaattcttg
     9481 tatttttttg aaaacaaccg ctccgtcttc ttgaataaac gtataaggct ccgctaaata
     9541 aacaggcgtt tgattgatta ctaagatttt ggctgacggc tggcgaaact gtaccagatc
     9601 acaaaacgga tgaacttgaa aactcgttcc aacaatgacg atcagctctg cttggctgac
     9661 ggcataagcc gcctgctcca tgactccatc ttgaaaccct tcaccatata acacgatgtc
     9721 tgggcgtatt tgtcctccgc atgcttgatg tttgtcgctg tgaagatatt ctttccagtc
     9781 taccgtttgt tgacattttc gacaatgaca ttgatacaaa cttccatgaa aattgacgag
     9841 ttgcgggctt ttagcctttg cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt
     9901 tttttctttc gctagatgtg ccatttccaa atgaatgata ttgggttttg cttcaggatg
     9961 ataaatcttt ttgacaaatt gatagaattt atccggttca ttcaccattg cttgatgact
    10021 cagcaagtat tccggctgct ccatcccgtg gtagattcct gacaaagatc gatagtctgg
    10081 cactccagaa ggtgtggata cccctgcacc ggtcaaaaat gtaatttgct gataagctaa
    10141 tatctctttt gctgcttcgt ctactgttaa ttgtttcatg ttttgtcacc tcactttcca
    10201 ttatagccca tactcgaaca aatttattgt tgaaaagtca gaaaaatggg aataaaaaag
    10261 tgttatgggc atttgaacaa tttatcacaa tgttcactga caaaagaatg aaaacgtgtt
    10321 acaatactgc tgtataaaag tgaggtgaat cgatatgtta ccattcttta tttattgggg
    10381 cgttattctg gcttgtatcg cctggttagc gttaagcgtt tatttctctg ttttttatct
    10441 tgtacgcaaa gaaaatggaa atctttgggc ttttgcttta ttcaacgtga ttgccgcaat
    10501 tgttttagcc attacattag ccgtatatcg aacatggggc tggggaatca cccaatactc
    10561 aagtttgatt tatctaatat tagccattta tggcgtcgtt gttcttttgc aagcgatcct
    10621 aggacgtgag ccaaagaaag cggcagctta aatgatcgag atggaactgg acacaggaaa
    10681 ttttctgttc tagttctttt tttattgtat tacatacatt tttgtgtata attatactat
    10741 cgtttgcaat gaatagatga ttcacatttt gattgttttt tcggtaaaag taaagatttc
    10801 tatatttatc agtatttttt caaaaaacag cagttataga ttgacaaagc ctctcgagta
    10861 tcgtaaactt tttttgtata aataactgaa tacgagatgc ctcaatcaat gaggtagagg
    10921 tcgcgagaat cattaaccct gtggagtgaa gcaaacatgg agaagcagga atagggagga
    10981 tcgccgaaat gtgcactttt ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc
    11041 ggactgtctt agcagtgatg ctaagttgcg ctatgttttg tgagaggttt atcaccttat
    11101 ggaattttag cggccttttt atatctataa atcggtcgct ttttattttt tcagataaag
    11161 ctttaccgca tttcatctag accgctcgtg tatcatataa tcagaaaggg agaaaaaaat
    11221 gaaaaaaaat atggtttcag ttgccgtact atttgctttt gtcttctcac tattcagtgt
    11281 gggatctctg gcaaatgcag atgaaaaaac accgagcgat gagtttcgcg taggtatgga
    11341 ggctgggtat gcacccttca actggtctca acaaacagat gagaataatg ccttgccaat
    11401 tcaaggccaa aatagttacg ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga
    11461 tggtttaggg aaaaaattag tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct
    11521 tcaatcaggc aagatcgacg cgatcatcgc cggaatgagc cctactgctg aacgacgaaa
    11581 agaaattgat tttaccgatc cttattacga atcacaatta gtagtagtag ttcaaaaaga
    11641 tagcaaatat gcaaaagcaa aaagtttagc agatctttct ggagcaaaga ttactgctca
    11701 gttaaataca ttccactata ctgtgatcga tcagatacca gatgtgcaga aacaacaagc
    11761 aatggataat ttctcagcaa tgagaacagc tttagcttct ggaatgatcg atggttatgt
    11821 cagcgaacgt ccggaaggtg taacggcaac tagtgtgaac aaagacttgg aaatgcttga
    11881 attcagcggt gcagatggct tccagacaaa cccggaagat gttcagattt ctgttggtat
    11941 gcgtaaaggc gacccggata tccaaaaagt aaaccagatt ctttctggca tctctaacga
    12001 tgaacgaacc aaaattatgg accaagcagt caaagatcag cctgctgcaa ctgatagtga
    12061 agaaaaacct ggcatcattt ctgatttcac tacgatttgg aaacaatacg gcaatatgtt
    12121 cttacgaggt gctggactta ccttgttcat cgcattgatc gggacagttg tcggtactac
    12181 acttggatta ctgattggtg tcttccgttc ggtacctgaa tcaggaaatg cactgactcg
    12241 cttcttccaa aaaatcggaa atgcacttct ttctatctat gtcgaagtat tccgcggaac
    12301 accgatgatg gtacaagcaa tggtcatctt ctacggtctg gcactcgctt ttggcatttc
    12361 attgaaccga acgattgccg ctttgtttat tgtctctctc aatactggag cttacatgac
    12421 agagatcgtt cgtgggggga tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc
    12481 cattggtatg acccatggcc aaacgatgag aaaagttgtt attccacaag ttttacggaa
    12541 tatcttgcca gcaacaggaa atgagtttgt tatcaatatc aaagatacag cagttttaag
    12601 tgtaatcggc gtagctgatt tgttcttcca aggaaactct gcttccggtg cgaatttcca
    12661 attcttccaa acatttacga tcgtcgggat catctactta gtcatgacct ttacgatcac
    12721 cagaatcctg cgggcggtag aaaagaaaat cgatggtcct tcagcttata ccaagcttga
    12781 agaaatcgac aattcaaatt tgcaggaata aggaggaaga atcatgaatg aaaaaattat
    12841 tgaaatcaaa catctaagaa aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt
    12901 aacagtcaat aaaggtgaag ttgtgacgat catcggttct tccggttctg gtaaatctac
    12961 tttcttgcgc tgtatcaatt tactggaaaa accaacaggc ggcgagatca tttataatgg
    13021 agaaaatgta ttagctccta aatataattt acctaaatat cgaacgaatc taggtatggt
    13081 tttccaatcc ttcaacctgt tcaacaatat gaatgtcttg gaaaactgta tgtctggtca
    13141 aacaactgtc ctgaaacgag ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga
    13201 aaaagttggt atggatcgtt atatcgaagc caaaccagca caattatctg gtggacagaa
    13261 acagcgtgtc gcaattgctc gggcattatc aatgaatcca gatgtattgc tattcgacga
    13321 acctacttcg gcgttagacc ctgaaatggt tggcgaagtg cttaatacga tcaaagactt
    13381 agcacatact ggcttgacaa tgcttatcgt gacgcatgag atggaatttg caaaagatgt
    13441 atctgaccgt gtcattttta tggataaagg tgtgattgca gaagaaggta caccagaaga
    13501 tatctttgtc catccgaaag aagaacgtac aaaagaattc ctttcacgta ttttaaatgc
    13561 ttaggatcaa tagctgattg ggacaaagag ctgcggtgtt taccgcagtt cttttttatg
    13621 aattactcat aaaaggtaaa tttctgaaaa ttttggaatc t
//
LOCUS       DAXWLG010000064        13559 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXWLG010000064 DAXWLG010000000
VERSION     DAXWLG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.064"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(81..1028)
                     /locus_tag="ACGE4T_001485"
     CDS             complement(81..1028)
                     /locus_tag="ACGE4T_001485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFF8386766.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1218..1487
                     /gene="rpsN"
                     /locus_tag="ACGE4T_001486"
     CDS             1218..1487
                     /gene="rpsN"
                     /locus_tag="ACGE4T_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8386767.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1663..2025)
                     /locus_tag="ACGE4T_001487"
     CDS             complement(1663..2025)
                     /locus_tag="ACGE4T_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8386768.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2036..3157)
                     /gene="alr"
                     /locus_tag="ACGE4T_001488"
     CDS             complement(2036..3157)
                     /gene="alr"
                     /locus_tag="ACGE4T_001488"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFF8386769.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3173..3523)
                     /gene="acpS"
                     /locus_tag="ACGE4T_001489"
     CDS             complement(3173..3523)
                     /gene="acpS"
                     /locus_tag="ACGE4T_001489"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFF8386770.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3653..5164)
                     /locus_tag="ACGE4T_001490"
     CDS             complement(3653..5164)
                     /locus_tag="ACGE4T_001490"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8386771.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5486..6838)
                     /gene="murF"
                     /locus_tag="ACGE4T_001491"
     CDS             complement(5486..6838)
                     /gene="murF"
                     /locus_tag="ACGE4T_001491"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFF8386772.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6991..8067)
                     /locus_tag="ACGE4T_001492"
     CDS             complement(6991..8067)
                     /locus_tag="ACGE4T_001492"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFF8386773.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8263..10008
                     /locus_tag="ACGE4T_001493"
     CDS             8263..10008
                     /locus_tag="ACGE4T_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFF8386774.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10288..10725)
                     /locus_tag="ACGE4T_001494"
     CDS             complement(10288..10725)
                     /locus_tag="ACGE4T_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFF8386775.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10709..12082)
                     /locus_tag="ACGE4T_001495"
     CDS             complement(10709..12082)
                     /locus_tag="ACGE4T_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386776.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12404..12892)
                     /locus_tag="ACGE4T_001496"
     CDS             complement(12404..12892)
                     /locus_tag="ACGE4T_001496"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386777.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12914..13516)
                     /locus_tag="ACGE4T_001497"
     CDS             complement(12914..13516)
                     /locus_tag="ACGE4T_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8386778.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3882 a         2941 c         2458 g         4278 t
ORIGIN      
        1 acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc agcttttttc
       61 gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc agcaggagga
      121 tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga cagattcatt
      181 gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc aaaggcttct
      241 gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt tttttgccgc
      301 aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt catgatctgg
      361 atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc tgcctgatca
      421 gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc tcctaatttt
      481 tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc cttttcatgg
      541 agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat tacagtatct
      601 gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc tggcgtgacc
      661 attccggctg cttcttccca actcatttta tctggaattt ttgctacttt tgctgatggc
      721 aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg atcacctaca
      781 tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc attacctggt
      841 acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca
      901 taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc gacttctctg
      961 ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat
     1021 cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgtctt tattttgaca
     1081 aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg
     1141 taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta ataaaaaata
     1201 attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa aaacagcaag
     1261 aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa gattacgaag
     1321 gcttgcataa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg
     1381 atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat
     1441 tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc aagtctggct
     1501 gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa gatacgttcg
     1561 cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac gcacagctgg
     1621 tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag taacaggagt
     1681 cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca taatgggtcg
     1741 atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga tctgctccaa
     1801 caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg
     1861 aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg
     1921 gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac
     1981 gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat cctccttatt
     2041 cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag tggatcgtac
     2101 ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg ccgataagcg
     2161 tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga tccatacata
     2221 cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc tgcatctttc
     2281 gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct tcaggagtga
     2341 tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact tgaatcaatt
     2401 cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg tgcccagagg
     2461 gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt tcatcatgcc
     2521 ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga agttttttca
     2581 gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc tgatctgctg
     2641 tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg atgaatgcag
     2701 ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca gtgtctgctt
     2761 ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca gctgctgctt
     2821 gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct acagtcagtg
     2881 cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct tctctcaact
     2941 cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca actgctgcct
     3001 cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca aacaattctt
     3061 ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct tttttatgta
     3121 tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct ttctagtctt
     3181 ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc catacattgc
     3241 cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa atctcgacat
     3301 cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc tctttgcaag
     3361 cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg aatagtttca
     3421 tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc agccgagtga
     3481 tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt tctcttttca
     3541 ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa ataagtccac
     3601 aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat gtttaatcgt
     3661 tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg ccatcttttt
     3721 ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa ttaccgccgt
     3781 cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat ccttttttct
     3841 gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct ggatcttttg
     3901 ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct aataatttat
     3961 ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg atttgttcaa
     4021 ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca
     4081 tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt ggtgtaacga
     4141 aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg tgaacgtagc
     4201 tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa atatctaatc
     4261 cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta aatgaacgca
     4321 atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca cggtaaccgc
     4381 gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg aagacgattg
     4441 ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt tcgtattctt
     4501 ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct ttgatcttca
     4561 catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc ggcatcgttg
     4621 ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt tcgatatctt
     4681 ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt tgaacggttc
     4741 caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta cccacaacga
     4801 tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg ccgtaaacgg
     4861 cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt gtttggatag
     4921 ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct ggatcgattt
     4981 tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt gcttgtccaa
     5041 taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc gttgcttctt
     5101 caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt tctttaaatt
     5161 tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc taccccgtcg
     5221 atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat
     5281 tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc
     5341 aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa gttgtttcta
     5401 tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta acatagtttt
     5461 tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct accacttcaa
     5521 ataagcccat gctgttgctg ccttttagca taatactatc tgttgctgca agattttgtt
     5581 tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt agttgggggt
     5641 atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg aaaatcatgg
     5701 cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa gactctttcc
     5761 ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc agatttttaa
     5821 aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg ctcaaaatag
     5881 ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa acatgggcga
     5941 gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta tacgcaatca
     6001 aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct ccttctacta
     6061 caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg ccatttccaa
     6121 taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc aacggttcat
     6181 ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc attttggctt
     6241 tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg
     6301 ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg tgatccatgc
     6361 ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg taaggaagac
     6421 caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca gacaagacag
     6481 acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca acgacttctg
     6541 cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa ggatctgcta
     6601 caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa aatgaagcag
     6661 atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta ccagcaagag
     6721 gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg
     6781 tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt agattcattg
     6841 agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga
     6901 ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa cgcggttacg
     6961 caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa aagattgacg
     7021 ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac cgtatttcaa
     7081 gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg gcatagagtt
     7141 taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc
     7201 gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt gataaacttc
     7261 ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt ttgcttcata
     7321 atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt
     7381 tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga
     7441 atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt
     7501 aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa gcaaagaacc
     7561 ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa gtactggtac
     7621 ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga ttttatccat
     7681 ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg tctctaagaa
     7741 gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc
     7801 ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct ctgtctgtcc
     7861 acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg ttagtaatgg
     7921 acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt aataattata
     7981 ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct ctgcgctgcg
     8041 tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa atcgttcagt
     8101 gatttactct catattttag cacaaaacaa aaataaacga ataacttatt tataaaaaaa
     8161 cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat tatttgtccc
     8221 gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca aacaaatcca
     8281 cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt gccaaacttt
     8341 gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg ccccaccttc
     8401 atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc cttcactcaa
     8461 acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt caaaaagacg
     8521 atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc aaaatcgcct
     8581 tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta
     8641 gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct tcaactattt
     8701 gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga tctaaaaggg
     8761 gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa
     8821 aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag ccccaagatt
     8881 tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg
     8941 aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat tcaaaacaag
     9001 cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca ggtgtttgat
     9061 gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt actaacaaaa
     9121 gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc
     9181 tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac catcaactac
     9241 atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact cacacaagcc
     9301 gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct tcgactttgt
     9361 cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa
     9421 cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac acatgtcgtg
     9481 acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt gctgaaagca
     9541 tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct catggaagtc
     9601 gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga tacattgaaa
     9661 caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc tcaacagctc
     9721 caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga attgaaaaag
     9781 caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt
     9841 ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt agaaaacgca
     9901 gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt cattattttt
     9961 ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg
    10021 acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt tctcaaattt
    10081 ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag aataaaaaat
    10141 gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca tggaacacat
    10201 ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat accttcgtta
    10261 cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa
    10321 agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc cagcttttct
    10381 taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt cgtcaaattc
    10441 ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag ctaaaccata
    10501 atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat aatccgcact
    10561 atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt tggtcgtata
    10621 atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta attttatgac
    10681 atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt ttcgccttct
    10741 tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg atccattttg
    10801 ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc gtgatcaata
    10861 atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa
    10921 actggtggat cacactccct ttattttgag tagtatactt gacaacgatc cggcgatcca
    10981 ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct gttactacgt
    11041 gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag aggaccaata
    11101 aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa
    11161 tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa tacataaaaa
    11221 tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat aaaaaaagga
    11281 aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc aacttccatt
    11341 ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag aaaccataaa
    11401 tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa agaaacaata
    11461 agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt cttttgatgt
    11521 ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc atgacgtaaa
    11581 aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt
    11641 gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc atattgataa
    11701 aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc catgctgcac
    11761 tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg cctaatattc
    11821 caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg taactatcta
    11881 gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca taaaacagcg
    11941 taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat aaaagataag
    12001 gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa
    12061 atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa ctaatctgct
    12121 cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt cttgtaaaaa
    12181 aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga ttactcaagc
    12241 agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca ggtgataaag
    12301 atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa ccagagtcct
    12361 tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg agagcttttt
    12421 tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa
    12481 tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga ttcactcgca
    12541 aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga aaaactgaag
    12601 aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa taaatttttc
    12661 catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt
    12721 aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa gttttcagca
    12781 tgtaatccac ttgtttttga ccaatgatcg gcacaaaagc ttccggccat acttcttgta
    12841 tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc atacacccac
    12901 gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc atgatcatgc
    12961 tatacacacc caccgaccat ggtttcaaga aaatcacaat ctgcataaat tccctaaaag
    13021 ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag ggaagcagca
    13081 aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt
    13141 tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag taacggacaa
    13201 gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa agcccaaatc
    13261 cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa ataggaatga
    13321 tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca aattgctgga
    13381 taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca taaagaatca
    13441 acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt tgtaaaactg
    13501 atcctttttt agacatctat attccccaca tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DAXWLG010000065        13007 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXWLG010000065 DAXWLG010000000
VERSION     DAXWLG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.065"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..610
                     /locus_tag="ACGE4T_001498"
     CDS             <1..610
                     /locus_tag="ACGE4T_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8386779.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACGE4T_001499"
     CDS             723..1001
                     /locus_tag="ACGE4T_001499"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386780.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACGE4T_001500"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACGE4T_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFF8386781.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACGE4T_001501"
     CDS             1458..2213
                     /locus_tag="ACGE4T_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8386782.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE4T_001502"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE4T_001502"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFF8386783.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE4T_001503"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE4T_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFF8386784.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE4T_001504"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE4T_001504"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFF8386785.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE4T_001505"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE4T_001505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFF8386786.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE4T_001506"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE4T_001506"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFF8386787.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACGE4T_001507"
     CDS             6950..7492
                     /locus_tag="ACGE4T_001507"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFF8386788.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE4T_001508"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE4T_001508"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFF8386789.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACGE4T_001509"
     CDS             9085..9987
                     /locus_tag="ACGE4T_001509"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFF8386790.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE4T_001510"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE4T_001510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFF8386791.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACGE4T_001511"
     CDS             11430..11852
                     /locus_tag="ACGE4T_001511"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFF8386792.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(12223..>13007)
                     /locus_tag="ACGE4T_001512"
     CDS             complement(12223..>13007)
                     /locus_tag="ACGE4T_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8386793.1"
                     /translation="SVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLAT
                     REAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGN
                     SHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQ
                     GYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYS
                     NGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT         4292 a         2318 c         2851 g         3546 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaagagaaa tcttagaata
    12181 atttggacag aacagaaaga agaaaacctc tttctgttct gtttatttgg ttaaaaatgt
    12241 tttgccatga cgaatcataa ttcgtagttt gaaattgtaa aagttgcgaa agccgtacgc
    12301 attgcgtttt aatactttga tgtggttgtt taagcattcc aatggtccgt ttgaatagct
    12361 gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta aagtggttgt
    12421 ataggtttct ggcagaccgc taatgtctct agttaaacaa tcctcaaata gtgactgatc
    12481 tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc gtaagttggg
    12541 acaagcagct aacagtcgat cggcaatctc actttcagtc aaataatctt taaaactggc
    12601 acgccaaacc cgtttttcat agtttaactt tgcttcttct ttttgtagga gtttccaata
    12661 gcgcttcaat tttttcccta accctcgttg agagtgggaa tttcctttaa gtcgtaccgt
    12721 ggtttctttc actcgatgat tcaagaaagt tctgccaata tgttggacga tgtggaaacg
    12781 atcgatgatc aactgagcat tcgggaaaag ctttttcacc aacgaaacat aaggcgcata
    12841 catatcaata acaatcaatt gaacggcttc acgagtcgct aatgaaaacc gagaaaaata
    12901 gcgttctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat ggctttgtcc
    12961 gtccatcatg ataaaactca ttgcactggc aacacttcga accgatt
//
LOCUS       DAXWLG010000066        12912 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXWLG010000066 DAXWLG010000000
VERSION     DAXWLG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.066"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            128..1414
                     /gene="efmA"
                     /locus_tag="ACGE4T_001513"
     CDS             128..1414
                     /gene="efmA"
                     /locus_tag="ACGE4T_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFF8386794.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVSYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1552..2151)
                     /locus_tag="ACGE4T_001514"
     CDS             complement(1552..2151)
                     /locus_tag="ACGE4T_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8386795.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2555..3676
                     /gene="mnmA"
                     /locus_tag="ACGE4T_001515"
     CDS             2555..3676
                     /gene="mnmA"
                     /locus_tag="ACGE4T_001515"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFF8386796.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3922..5343
                     /gene="arcD"
                     /locus_tag="ACGE4T_001516"
     CDS             3922..5343
                     /gene="arcD"
                     /locus_tag="ACGE4T_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFF8386797.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5357..6475
                     /locus_tag="ACGE4T_001517"
     CDS             5357..6475
                     /locus_tag="ACGE4T_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFF8386798.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6535..7827)
                     /locus_tag="ACGE4T_001518"
     CDS             complement(6535..7827)
                     /locus_tag="ACGE4T_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8386799.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7977..8537
                     /locus_tag="ACGE4T_001519"
     CDS             7977..8537
                     /locus_tag="ACGE4T_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8386800.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8666..9043
                     /locus_tag="ACGE4T_001520"
     CDS             8666..9043
                     /locus_tag="ACGE4T_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFF8386801.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8976..11048
                     /locus_tag="ACGE4T_001521"
     CDS             8976..11048
                     /locus_tag="ACGE4T_001521"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8386802.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11259..12206)
                     /locus_tag="ACGE4T_001522"
     CDS             complement(11259..12206)
                     /locus_tag="ACGE4T_001522"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8386803.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12452..>12912
                     /locus_tag="ACGE4T_001523"
     CDS             12452..>12912
                     /locus_tag="ACGE4T_001523"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFF8386804.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQ"
BASE COUNT         4165 a         2340 c         2754 g         3653 t
ORIGIN      
        1 tctaagtaaa gaaatttaaa ttgaaacgat tgttatttag ggaaaaaggt attttttgaa
       61 aaaagaatcc cgtataatga ttgttgtgca aacgctatac aaaatgaaaa agaaaagagg
      121 gatgtttatg gaaaatgaac agtcggtcgt tttaacgaat tggaagagaa attatttgtt
      181 ctttttatcg ggacagtttt tgtcaggaat cactagtatg gtcgtccaat atgcgatcat
      241 ctggtatttg acaagagaaa ccggttcggc gacaattttg agttttgcta ctttactagg
      301 aatgattccc atggttttac taagcccatt tgtaggaccg ctagtggatc gctgggataa
      361 aaaagctctt ttgattgtca cggatattat tgtagcgatt ttcgcgctta ttttagcagt
      421 agtcggaacg atttcagaat ccttcccgat ttggctagtt tttgtgtctt tgtttatgcg
      481 ttcggtcgca caaacattcc aaatgccaac gatccagtcg attatgccga caatcgttcc
      541 atcttctcac ataacaagga cgaatggaca gttaggaatg gtccaatcag cgaatttcat
      601 cattgcgcca gctctaggtg cggctttgtt ttcagtcgtt cctgtcagtt accttatttt
      661 attagatgta ttgggggcag tttttggtgt cggcttattg atttttgtga agatcccaaa
      721 agtttctcct gaaatattgg aagttccgct tactattttt aaagatgcaa aatttgggct
      781 gcagcaattg atggataata aagggttatg gtacataacg atcaatggtg catttgtgat
      841 gctgttgttc atgccagcaa tcagtctgta tccattgatg acgctagatt actttggtgg
      901 ttccgttgga caggcgggag cagtggaagt agtctacgca gtcggaatgc tcttaggcgg
      961 tgcactgatc agttttatag gaacatggaa agaccgaatg aaacctatta ttatagccta
     1021 catcattatg ggcctgacga tcggtgcaag cggattggtt ccaaacgata gtcaagggtt
     1081 cttgtacttt cttatcttga atgcaggagc aggttgtgca acgccatatt tcaatacact
     1141 gctgatggca atgatccagc agagttatga atcgaatgtt ttaggtcgtg tcttagggaa
     1201 cttcaactct ttgatgaacc tggcaggtcc aattggatta ttatttgcag gaccattagc
     1261 tgatcgttta ggggtagaaa aaatgttctt gttttccgga atcgggattt tattatgcgg
     1321 aatcgtttta tttttaacgt cagctgcccg aaattatgat aaagaattac agaaaaaact
     1381 ggtaaaagaa caccatgagc aaaaagacga atagtatgga atggagttgt gacagaagtc
     1441 ttgttacaac tccatttctg atcctaatgt tcctgtggca ataagcctac tcaaatgaaa
     1501 aatatgagta attatcttta tttgtttaat acttgatata gggcagtttt ttcacgacct
     1561 atcgtacttc ctctttgctt catagatttc tttgatttgt tgctcagata acggagtgcc
     1621 aaatggtagt ttttccttta agagttctct ttccggtgcg tctacgccta cattgacatc
     1681 gttctcgata ggtacgctgt aatgatggat atgtccatga agattgcgga tagtgtcgtt
     1741 aggcccgagc aacatcggat aatgagtcaa gtaatattgt tgatggtcga atttaacgat
     1801 ggcacctaca tcataaaaat aaaatttggg acttccgtca gtaagcctag gatcattttt
     1861 ttctaaataa cggaaaaaag cgcgactgtc atggttccct ttgatcaagt ggattgttcc
     1921 ttgcagttga gtgaccagtt ccaaaatttc tgcatttgct ttttcatatt gcggatgcat
     1981 agcaaggtcg cctagatgat aaacatggtc ggtttctttt attactgcat tccagctagt
     2041 gatcaactgt tggttcattt catccacatt tgcaaagagc cgcggagcaa agtcgttttt
     2101 tcctaataag cgttcatgga aaaagtgcat atcactgata aagtaattca tgtatctttt
     2161 gcctcccttt tttcttagag taaacaaaat cacttatctg agcaactcat aacatctaaa
     2221 aagaaaaggt tgtaaatgag acgtgcagct ttaagcgata agaagcaatt ttgaaaaata
     2281 gtttttcaca atttataaaa ttattcctta ttgccgaaag gctggccctt tttatgagta
     2341 tgccaatttt ttatatcgaa tgctcaaaag atgagtgatt ttttggttga ctatcatgtt
     2401 tgtgtactga ataggtccag tagaacagta tttttacgac ttgataatta agggatgaaa
     2461 ttttcagaaa tatgacgtat aatgtaaaat actgaaaagt tgcgaccttc aactcgtaga
     2521 tgtttcagaa tctaattgaa atgatggtga tatcatgaca gacaacagca aaacacgcgt
     2581 tgtcgtaggg atgagcggcg gtgtcgactc atctgtaaca gcacttttgt taaaggaaca
     2641 aggatacgac gtgatcggaa tattcatgaa aaattgggat gacacggacg aaaacggtgt
     2701 atgcacggcc acggaagatt ataaggatgt agcgaaagta gctgctcaaa tcgggattcc
     2761 ttattactct gtgaattttg aaaaagaata ttgggatcgt gtatttgaat atttcctagc
     2821 cgaataccgc gcaggacgta cacctaaccc agacgtgatg tgcaacaaag aaatcaaatt
     2881 caaagcattc ttggattatg caatggatct tggcgccgat tatgtagcta ctggacatta
     2941 tgcgcaagtc actcgcgatg aaaacggcgt ggtccacatg cttcgtggag tagacaataa
     3001 taaagatcaa acttacttcc ttagccaatt atcacaagaa cagctttcaa aaacgatgtt
     3061 ccctctaggc ggcatggaaa aatcagaagt acgggcaatt gctgaacgcg caggccttgc
     3121 gactgctaag aaaaaagatt caacgggaat ctgctttatt ggtgaaaaga acttcaaaca
     3181 atttttgagc aactatctgc cagcaaaaaa aggaaatatg gtcactcttg atggtgaagt
     3241 caaaggacaa catgctggct tgatgtatta tacgattggt caacgtcaag gactagggat
     3301 cggtggcgga ggagattcgc aagaaccctg gtttgttgta ggaaaagatc tagcgacaaa
     3361 cacattatat gtggggcaag ggttccatca tcctgcattg tatgccacaa gcttggatgc
     3421 aagtgaaatc cactttacga cgaatgaacc aatgccaaaa gaattcaagt gtacagcgaa
     3481 attccgttat cgtcagcaag acgttcctgt gacagttcgc ttattggatg ataatcgtgc
     3541 agaagtcgta ttcgacgaac ctgtacgtgc aatcactcct ggacaagcag ttgtatttta
     3601 tgatggaatg gaatgtctgg gtggtggttt gatcgaccac gcatatcaag aaacaaaagt
     3661 tttacaatac gtttaagcga tattaaatga atcaaaacag ctatggctaa caaaagaaac
     3721 accttttctt ttggatagca tagctgtttt tttgcaataa aattaatttt tattcatttt
     3781 atacttgaaa tacttgtgta aaaagttcat aatatccaat aatatgaata aagttgcttt
     3841 ttgttaagca agagcagttg caaaatagaa gggaatcttt tgcaagtgat ttgttcaatt
     3901 ttataagtga gggtgatgga aatggaaaag aaatcgacgg gtattagcaa atggggactg
     3961 gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt ttgggatcac aagtgatctg
     4021 gctagttctg cagctccagg gccagcgatc ctttcatgga taatcgttgg gattggtatt
     4081 ttagctttgg tattatcgct gaatcattta ggagaaaagc gtccagatct agatggtggt
     4141 attttcggtt acgcggaagc ttcatttggt aaattaggtg gcttcatcag tggttggggt
     4201 tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa cgatgctgat gagcgccatg
     4261 ggagaatttt tcccgatatt caaaggtgga caaaatgttc cttctatttt atttgcaagt
     4321 atttttattt ggggtctgac tttattagta aacaatggag ttgaaggagc gagcgtcatc
     4381 aatacaatcg tgacgatctg taaattagtg ccattattcc tatttttaat atttgtattt
     4441 atcgcattta aagtgaatat attcactgct gatttttggg gtaacgtatc tgataatcta
     4501 gtgaatggaa cagggcagca aggaacgatc tggttgcaga ttaaaggttg tttgatggtt
     4561 atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt tagccaaccg cgcgaagaaa
     4621 cgttcagaag cccagcaagc atcgatcatt ggtctgcttt gtttattgtt tatctatatc
     4681 cttgcttcga tcttgccata cggcgtctta tctcaagaag agttagcaac tatttcacaa
     4741 cctgctatgg caaatatttt aaaaggaatc gtgggaaatt ggggagcagc gctgatcaat
     4801 atcggcttga ttatttcgat cattggttct tggttgtctt ggacgatgct cccagcagaa
     4861 acaacgatgt tgatggctcg agacggtctg ttgccgaaag gttggggaaa aacgaataaa
     4921 aaaaatgccc caacttattc gttagttgtt actgcaatct tgactaatct attcttattg
     4981 actttcttag ttactgatta tgcctatcaa tttgcctatt ctttatgtac agcggcaatt
     5041 ttgatttgtt atctacttgt cgggatctat caagtccagt tttctttaaa agaaggaatc
     5101 aaaaaacaag tagtgatcgg tgggattgct gtagtttttg aattgatggg catcatttta
     5161 gcaggatttt cttatgtcct tttatgtagt attgcctatc ttcctggttt ttgcttctac
     5221 tggatcgctt gtcgtgaaac caaacatcaa attacgggaa aagaaaaagt aatgattggg
     5281 ctgatttgtg tggccgcact gctttcaatc gggttgcttg ctgtaggctg gatcaagata
     5341 taagggggaa agaaacatga aaatcgcaaa tttcggtgta gaagaatggc taaataaatg
     5401 ggaaaagaaa gcggtatatg atattgcaca aagctcgatc gaagcattga cattagacga
     5461 actcgttggc ttagatggga ctagcgtaag taaatttttc gaaaaacgca aaaacgaacc
     5521 attggactat ggctggatcg aaggatcaga tgttttcaaa caagaagtcg cttctcttta
     5581 tcaaaccgtc gatccggaaa acatcttaca aacgaatgga gcgactggtg ctaatctttt
     5641 agctctatac gcgttagtgg aagcaggaga ccatgttgtt tcgatgcttc ctacttatca
     5701 gcaattatat gacatcccca aatctttagg tgccgctgtt gactttgttc atttgaagga
     5761 agaagaagat tggcagtttg atttagaaca gctagaagcc ttagtcaaac ctgaaacaaa
     5821 aatcatctgt ttgaatagtg caaacaatcc tacaggcaca ttgctagatc ggaaaacttt
     5881 agaaaaaatc gcagaaattg ctcgtacagt agatgcatat gtattgatag atgaagtata
     5941 tgcgccttta acggataaag gtgaattctt gtcgatcgta gatgtctatg aaaaaggtat
     6001 agcaaccaat tccctatcca aaacctactc cattccaggt attcgtattg gctggacagc
     6061 tactggacct gagttggcag aagtttttcg aaagtatcga gactacacga tgatttgcgg
     6121 cggagtcctg tcagatgatc ttgctgtaca tgcattgaag aataaagaaa aaatattggc
     6181 gcgaaacaaa aagatcatta cagagaacct tgtgatttta aaacaatggg tagccaatga
     6241 accgaaagtt gaacttgtcg ctcccaatta cgtctctact tctttcatta aattagccat
     6301 cgaagaagat gaccagacat tttgtatcaa tttattagaa gaaacaggag tactattagt
     6361 accaggaagt gcctttgatt taccgaagca tgcccgacta ggttactgtt gtaaaaaaga
     6421 aaccttggaa aagggattat tcctcttatc tgagttcttg aaaaaacagg cctaaacaaa
     6481 aacactgcaa ggacagtctg tgacaactgt tcaagcagtg tttttcattt acgcttattg
     6541 tctatgagaa ggagagtgcc ctgaagctat ggagactcct gaagcttttt cttctgcagt
     6601 ttcttgctga tggatctctt gtgtattgtg tcgtacaagc aagaagttga ccaaaacaag
     6661 aagagaagtc gaaagaaaga cgccacgata accaaatgca ctggaaacac tggagccgat
     6721 cataggccct actacgttac cagttgcttg gaaagattgg ttataactga agatccgtcc
     6781 agcagcttga tgaggcgaat attttgtgat caatgcttgg acagcaggga gcagacaagc
     6841 atcagagata ccaatcaaaa aacgtaaggc agctaattgc caaacattcg tgacgaatgc
     6901 cattgggata taaaccagaa tcgcaaaacc caaaccaatc gcaagtattc tttcactccc
     6961 aatcttgtca cctaagcgac caaatcttgg agcggctatc aaagtggcaa tcccaggtat
     7021 cgaagcaatg attccgctga tcaacgtaac attcccatgt ccatgcagca attggcgaat
     7081 gtacagactg atgattggac taatggaatt gttggatgct tggatgatca tcgttgtgac
     7141 gaacataccg atgacgacgt gtggatattt caagtctttg aaaatctgtt tggcagaaac
     7201 catgttttct tttttcactg ggacaaattt ttcatggacg aaaaacagac tcaacaagaa
     7261 gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg cgatagccaa acgctgaagc
     7321 agagatcccg ccaagaagcg gtccaagtaa tgttcctgta acagaaccag ttgctaaggt
     7381 tccaagaact tgaccacttt tttcttttgg cgtcccagtt gcgacaaggg cagtggcatt
     7441 actgatataa ccagaaaaaa tcccttgaag cagacgcaat gcgactaatt gatacacact
     7501 agtcacgcaa cccatcaaag aaatgacgat tgccattcct aatgaagcac gcaacagcat
     7561 caattttctt cctttttggt cggctaattt tccccaccaa ggagagataa gcgtagtcac
     7621 caagaaagta gatgaaaaag tcaatccgct ccaaaagttc aattgtgacg tactataatt
     7681 acctaacgta tctatgtaaa gtgacataaa aggcatgact aaactaaatc cgataccagc
     7741 cataaatgtt ccaaaccata agacaaacaa gttttgttcc caagtttctc ttttcctaaa
     7801 taatttttcc tttattcttg cagccaatta gactccttct ttctgaaaat tttcatctac
     7861 agctctttat tataaactac aatatgtagt ttatcaatga aagtctcttt tttctctttc
     7921 tttttcgtgg taaactgaaa gagtgaagga tttattttat gagaggaaac atggaaatga
     7981 aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt tcgagaacta gcagaagaga
     8041 tggatgttca gcaaataacc tttcagcatt tagccaaacg cttggatatc aaggctccct
     8101 cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac agcgctaaca gcgaaacttc
     8161 tgcaggaact taatgaccag ctgcgacggg ctcttgtggg aaaaagtggt caggaagcac
     8221 tgcagatcta tgcagaaact tatcgatcct ttgctttttc aaatcaagca gtgtatgagt
     8281 tactgatcag tgtaccgcat accaatgaag ctgttttatt agatggaatc catgaaacga
     8341 atcagatcat tttgcagata tttgaggctt ttcaattaac gaaagaagaa aaagttcatc
     8401 gaagcaggga acttcgtagt atgatccacg gttatctttc cttacgtttt ttaggatact
     8461 ttacaaaaga accaacaatc gatccagaag acagctattt ttggatgatc aataacttta
     8521 tcgctacttt gccttaaaca atcagattca tttgtaacaa ttttaagatg ttcattaaat
     8581 gaccctttta taagagaaaa ttcttgcaac tttcttgaaa agtgactaag atgagtctag
     8641 aaaagctgaa agaaggagca cgagtatgta tcaagtaatt accatgttcg gggacaatga
     8701 gccttggtgg ttttttgaag actgggaaga agatattgaa gaagaagaaa cattcgagtc
     8761 tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac gaaatccatc aaaattatga
     8821 atatatcaat gcaaaaagta actttctcag cgccttttgg aatgacggag atgagcgctg
     8881 gtgtgaagag tgtgacgatg atttacagca atacaaggga cttgcgttgt tgaaaaatca
     8941 tcagcccatc acagtagaaa gcagaaagga attttatgaa acaactaatt ctagcggaaa
     9001 aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc taatcaaaag cacaaaaatt
     9061 attatgaagg acccaaggtg atcgttactt gggcccttgg tcatttatta ggattgaaaa
     9121 tgccggaaga tctgaataag gaatggcaga gctggcaaat ggacacgttg ccaatgatcc
     9181 ctaaaaaact aggaatcaaa ccacttccta aaacaggaca tcaattaaaa gcaatcaagc
     9241 agctggctca gcgaaaagat gtctcagaag cagtcattgc gacagatgcc ggaagagaag
     9301 gggaacttgt tgctcgctgg atcttagagt atgtgcattt caataaacca gtgaagcggt
     9361 tatggatctc ttctcaaaca gataaagcaa tcaaaactgg attcaagcaa ctaagacctg
     9421 cgaaagaata tgatgcattg tatgaatctg ctcttgcccg ggcaaaagcg gactggctgg
     9481 taggattgaa tgtgaccaga gccttaactg tgaagtacgc ggataattta tcagcaggcc
     9541 gtgtgcagac accgacacta gcattagtga gagaccaaga aaagaaaatc gagcagttcc
     9601 gtccacaaac gtatttcacg atcacgttgc aagctggaaa agaacaagcc aaaatgcttc
     9661 aaaaaaatca atttgcttta aaaacacaag aagaagcaca aaaacttgtt caattgatca
     9721 gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt aaaaacggaa tctgcgccat
     9781 tgccttatga tttaacagag atccaaagag aagccaatca acgctatggc tattctgcaa
     9841 agaaaacatt aagtcttgta caaagtttgt atgagaccca taagatcgtt acttatccgc
     9901 gtacagacag caaatatctg acaaatgaca tgaaaagtac gatgaaagaa cgattgcaag
     9961 cagtcagcga ctttgctcct gaggtcaagc agtatttgaa aaacggagcc atcgtccaac
    10021 agacaaaagt attccaagac agtaaagtga ccgatcacca tgcacttttg ccaactgaaa
    10081 acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa aatctatcaa atgattgtct
    10141 ctcgtttttt aggactgttt gctcaacccc ataaagtcag ccaaacaaaa gttacggttg
    10201 aatttggtaa agaacgattc atttttcggc aaaatcgtgt cgtccaagca ggatggaaag
    10261 gcgaaactga aactgagacg gaaacagtca aatgggaaaa aggaatgaga atcaatcccg
    10321 atttcgccat caaaaaagaa ctatctgcac cgcctaaacc attgtcagaa gcaagcttgc
    10381 ttggtcaaat ggaaaaatac agtctgggaa ccccagctac gcgtgcagag attatcgaga
    10441 aactaatcaa atctgaactg atggaacgta cccctcaagg attgcaagta tctcctaaag
    10501 gtaagcaatt attagtattg gtaaatccct ctttagtaac accagatttg acagaaagct
    10561 gggaaaaaga ccttgaagct attgctgcag gaaagaaaca agcaggaacc ttcttgaatg
    10621 gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa atcaagcaaa caagaatatc
    10681 aagatttctc gattacccaa aagaaatgcc cagaatgtgg ggccaatctg cgcgagaaaa
    10741 acacacgtga cggcaaaatc tatgtctgca ccaattcaga ttgcaactac cgcagaagaa
    10801 aagatccaaa agtctctaat catcgatgcc cgcaatgtca ccggaaaatg gaaatcatcg
    10861 aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg aacgacagaa aaattgcttg
    10921 ataaaaaaga gcagaagaag aaaatgacca aacatgaaga acgacgcttg ctgaaaaaat
    10981 atgcacaagc agaagaacca gaagaaagcc cattagcagc agcactaaaa gcagcaatga
    11041 agaagtaata agatatgaaa gaggttgtga tagaagtggt cagcttcaag aaataagaag
    11101 gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgaagaagct
    11161 acttctggaa caccatttat tcggaaaaaa ggactgtgac aagacttttg tcacagtcct
    11221 ttttcattta aaaaaatgtt tacttcattt gacgtatatt attggtgagg cttcaattca
    11281 tttggcttca ctttttctgt ttctcttatg atataaacag gacgctgttt cgtttctaag
    11341 aagattttcc caatatattt cccgatgatt ccaagacata acaattggat tccaccaatg
    11401 aacaggaaga tcgagaccat ggaaggccat cctgatgtag gatcaccaaa caacaatgcc
    11461 cgcaaaataa tgaacaaaat tgcaatacca gaaccgacac aagacaaagc gccgataaat
    11521 gaagcaatcg ttaacggtgc atccgagaag ttcacgatac catcaatcga gtagttgaat
    11581 aatttccaga aggaccaaga tgtttcgcca gccacacgtt cgcggttttc ataagaaata
    11641 tattctgttt taaatccgac ccagctaaat aatccttttg aaaatcggtt gtattctgtt
    11701 aattctagga cggcatccac catttgacga gtcatcaagc ggaaatcacg agcgccatcg
    11761 accatttctg tatcgctgat tttattgatc aactggtaga attttttcgc aaagaaactg
    11821 cgtatcgcag gctcgccttc acgagtcgaa cgccgggtgc cgacacaatc gatatcagaa
    11881 gtagtgatcc ggttgatcat ctcaggaagc agttcgggcg gatcttgcag atcaacatcc
    11941 atcacggtca ctaaatctcc tgtagaagct tgcaatccag cgtacagagc cgcttctttc
    12001 ccaaaatttc tggaaaatga gacaaagcgg acataaggat aagctgaggc tagcttcttg
    12061 attgctgcta acgtaccgtc ttttgatccg tcgtcaataa acaaatattc gaactcatgg
    12121 gcacctttga atttttcaac ctcatcaaaa aaaagaggaa cagttttttc ttcattaaaa
    12181 catgggacaa caattgatac tttcatcgtt tctatccttt caatcgtatg tttatatatg
    12241 gtttacattc aaaatttaat aaaccttaaa taacaccttt aaaatttgat tttacgtaaa
    12301 agaacaggta ttctttttgt aaactaacgt atttagttta ccatgtttcc tcggtttatc
    12361 aaacttttaa gaacaatttt tattagttgg caagtagttt tggatagaga aaaaggagga
    12421 agttccagtc tattgactag gagcaaacaa aatgaaaata cacacaccta tcggcttttt
    12481 atggttagat gcagatgaat ccggattgac tgctgtatct tttcagccaa tttcagaaag
    12541 cgaatcagcg aatcagcgaa ttttaacgcg cacacaagaa cagtttgaag aatattttgc
    12601 gggaaaaaga aaagaatttg atgttccttt atctataaag agaggtacgc cttttcagca
    12661 aaatgtctgg aaagctttgt cgaccattcc ttatggagaa atccgaacgt ataaagaagt
    12721 agccgttgct gttgatagtc cgaaagctgt gcgagcgatt gggcaggcaa atcgaaccaa
    12781 tccattgcca atcgttattc cttgtcatcg agtgatcggg caaaatggaa aactgacagg
    12841 atatatggga aacgcaatag atggggttga aatcaaacgc caattgttgt tgttagaagg
    12901 atatctgcaa aa
//
LOCUS       DAXWLG010000067        12822 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXWLG010000067 DAXWLG010000000
VERSION     DAXWLG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.067"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(89..649)
                     /locus_tag="ACGE4T_001524"
     CDS             complement(89..649)
                     /locus_tag="ACGE4T_001524"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFF8386805.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(671..2779)
                     /gene="mutL"
                     /locus_tag="ACGE4T_001525"
     CDS             complement(671..2779)
                     /gene="mutL"
                     /locus_tag="ACGE4T_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFF8386806.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            complement(2797..5442)
                     /gene="mutS"
                     /locus_tag="ACGE4T_001526"
     CDS             complement(2797..5442)
                     /gene="mutS"
                     /locus_tag="ACGE4T_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8386807.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            complement(5426..5839)
                     /locus_tag="ACGE4T_001527"
     CDS             complement(5426..5839)
                     /locus_tag="ACGE4T_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8386808.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            complement(5817..6614)
                     /locus_tag="ACGE4T_001528"
     CDS             complement(5817..6614)
                     /locus_tag="ACGE4T_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFF8386809.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            complement(7022..7795)
                     /locus_tag="ACGE4T_001529"
     CDS             complement(7022..7795)
                     /locus_tag="ACGE4T_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8386810.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            complement(7815..8450)
                     /locus_tag="ACGE4T_001530"
     CDS             complement(7815..8450)
                     /locus_tag="ACGE4T_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFF8386811.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            complement(8478..9329)
                     /locus_tag="ACGE4T_001531"
     CDS             complement(8478..9329)
                     /locus_tag="ACGE4T_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFF8386812.1"
                     /translation="MRGHVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            9591..10202
                     /gene="rpsD"
                     /locus_tag="ACGE4T_001532"
     CDS             9591..10202
                     /gene="rpsD"
                     /locus_tag="ACGE4T_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFF8386813.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            10342..10881
                     /locus_tag="ACGE4T_001533"
     CDS             10342..10881
                     /locus_tag="ACGE4T_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8386814.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(10951..11460)
                     /locus_tag="ACGE4T_001534"
     CDS             complement(10951..11460)
                     /locus_tag="ACGE4T_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8386815.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(11841..12602)
                     /locus_tag="ACGE4T_001535"
     CDS             complement(11841..12602)
                     /locus_tag="ACGE4T_001535"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFF8386816.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            complement(12599..12820)
                     /locus_tag="ACGE4T_001536"
     CDS             complement(12599..12820)
                     /locus_tag="ACGE4T_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFF8386817.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
BASE COUNT         3475 a         2855 c         2281 g         4211 t
ORIGIN      
        1 ttagtcagag tagataagta atcttttcaa taaaaaaaac gggctatcat ggattttcaa
       61 tcgaaaatca gatagtccgc ttttttgttt aaatgaatgt tttcagtgcc tgattgacag
      121 caccaatcgg aaatcctacg atactgtaat aatctccaga aatttctttg acgaatacac
      181 tcgcagcatt ttggatgccg tatgcccctg ccttatccgc ataatccccc gtctccaagt
      241 agcgtttgat ctcttcgtct gtcaaaggat aaaaagtgac atgtgcttct gctagtatcc
      301 gttcttctcg ttcctttgtt tttaaaacaa cagctgtata tacagtatgt gtttccccgc
      361 ttagtttttt cagcatgaat tcagcctcag ccagatcttc tggtttaccc atgatctctt
      421 gattgagaac aacagtcgtg tcactggcaa tcactaaatg gtcttctttt tcatcttgtt
      481 cccagacaat tcgtgctttt tcttcagcca ttctacgaac ataatcgacg ggctgttcgt
      541 ctggaaatgg cgtttcatcg atatcagcag gttcaatctc gaattctgaa acgatatgag
      601 ccaataactc ttttcttctt ggtgattggg atgctaatac aatcttcatg ttttccctct
      661 tctctttgac ttaatgcggg tcttggattc gtttgaacat cctttccata tccgaattgg
      721 taaagtgaat caatactgga cggccatggg gacaattgaa aggattttcg catcgtgcca
      781 gatccttcag cagcacacgt gcttgtgctt cattcaaata atggttggct ttaatcgatc
      841 gtttgcaact catcatgatt gctgttgctt cacggaattt ttttacactc acacttccag
      901 ttgtcagcag catatcgatc atctcccgaa taatcgattc ttcttctcca ttgggatacc
      961 aagtaggatg ggcccgtaca ataaagctgt tttgaccaaa ttcttctaaa taaatccctg
     1021 catcttctag caagtgtttt tgctctttta atttcaaagc atcactgttt ggatagtcta
     1081 acacaaatgg aacaagcaat tcttgcagat catcagatac ttccccaatt ttttcccgga
     1141 aatattcata cttaatgcgc tcttgggcag catgctgatc aatgatgtac aacccatcgc
     1201 tactttgggc aaaaagatac gttccgtgca tctgtccaaa atattccaac tgaggaaaac
     1261 gttgtactgg tttttccgtt tctaatttct tgatggcttg ttctaatgtt ttgtcgtttc
     1321 cttgagtgaa atcaaattca ggatggagtg tcaattcttc ctcgtaaatc ttttcatcct
     1381 cttctacgga tttatctgct tgttcttcat ttgttttctc catgttatcc acaacgggaa
     1441 attctttctt ttcagatttt tccactgttt tttctacaaa gttatcaaca ttttcaccaa
     1501 acttatccac cgttttcgct gttttttctg gataagtggt gttttcttca gaaaaaccag
     1561 aatagttttc aacattttcc acagacttat caacaattct gtcaacaaat gtgttctttg
     1621 gttcttctac gaaaaaggta cctgtctctc gatcaaaatt caaactactt ggctttttag
     1681 gtttttcttc ttcaaaagcg taggacaaat cgatttcgat ttgttctgtt ttaggctgat
     1741 caggtacttt tttcttaaac cgtagattat ccgctgcact tggaatcaga ttttcttgag
     1801 acaatgcttt ttgaatggcg ttactaatta gctctgttag ttctttttcc ttcgacagcc
     1861 ggacttcctg cttcgtcgga tgaacattta catctactaa aagcgggtcc attttgattt
     1921 ccaatacagc aatagggaaa cgaccgacca ttaattttga cccataacct gcaacgattg
     1981 ctttattcaa ggaaaagttc ttgataaaac gtccattgat gatcgtcgat aagtaattcc
     2041 tactggcacg agtcacctca ggcaatgaaa catagccaaa taactcgaaa tctagatctt
     2101 ttccttcgat tttcagcatt ttttttgcgg tagataatcc gtagattccc gcaattgttt
     2161 gtttcagatc gccatttcca gcagttgcca gcattttgtt tccatcatgt actaaacgaa
     2221 aagcaatcga tggatgactc aatgccagtc gattgacgat gtccccgatg ttcgctaatt
     2281 ctgtgtgcag tgttttcacg tatttcaagc gggctggcgt attgaaaaac agattgctga
     2341 cggtaatctt cgttccttga cgcagtggtg ctggtcgatg ctcaataact tctccgcctt
     2401 tcagcgacag ataggtgcct tgtttttctt cctgattcgc cgtttccaga gtcatttcag
     2461 aaacagaggc aatacttggc aaagcttccc ctcgaaagcc cagcgttcgt atgcgaaaaa
     2521 gatcgtcgcg attatggatc ttacttgtcg catgccgctt gaaggcattc tctacgtcgt
     2581 ctgccaaaat gccttctccg ttatcaatca cttggatcgt ccgcagtccc gcttcttcaa
     2641 ctaagatatc aatctgagta ctgccggcgt caattgcatt ttccactaat tctttcacaa
     2701 cggaagctgg tcgctccact acttctcccg cagcgatctg attagctaaa cgttcagata
     2761 attcttggat tttggccatc gatatccgcc ttcttcctac agttgttttt gtaattcata
     2821 taatgtgttt agtgcatcca ttggcgtcat ttctagtaaa tttatttgct tcagtgattc
     2881 aatgacttct gtttcatttt cggataattc actgaataaa gaaagctgtt ggttttcttc
     2941 ttttacacgg tcttgtatat ggtcttgatt tactttttct gcaggtggtt ttatttgctc
     3001 ctgtttggct gttttagttt tctcttctac ttcagctgga acagtatggt gtccgttatc
     3061 gccattttct agcgtatgca ggatatctgc tgctcgttct agtagattgg aaggcaatcc
     3121 tgcgatcttt gccacatgta tgccgtagct tttatccgca ggtccatcca tcattttatg
     3181 caagaagaca acttctccgt ctttttcaac ggctccaaca tggacatttt taagctgcgg
     3241 aagttctttc tcaagtaccg tcagttcatg ataatgagta gaaaagagtg tttttgcttg
     3301 tacatgacga tggatatatt cgatgattgc ttgggccaac gccattccgt cataggttgc
     3361 tgttcctcgc cctagttcgt caaacaaaat caaactgttt ggcgtagcat aacgcagggc
     3421 ttgattggct tccatcatct ctaccataaa agtactttgt ccagcaatta agtcgtcact
     3481 tgcaccaatt cgggtaaaaa tccgatcaaa aataggtaaa actgcttgtt ttgccggaac
     3541 gaaacagccc atttgcgcca tcagaacagt taatgccaac tgtcgcatat acgtactttt
     3601 accagacata ttcggtcctg taatcagcaa gatcatttcg tcttctgcca tttctacgct
     3661 gttagggata tattcctgat gtcctaatac tttttctact acaggatgtc gtccgtcttt
     3721 gatcagaagc tgatgcttgt cactgacaag ttctggccga acatactgat agcgttcgct
     3781 gatcgttgca aaactttgta gcacatctac tgcactgatt gcttttgcaa gtacttgtag
     3841 cggctggata gcctttttga cttcttctct cacagctaaa aacaactgat attctaaatc
     3901 aacagatttt tcttctgctt cgaggatttg tgtctccaat tcttttagtt ctggtgtgat
     3961 gaaacgctct gcatttgcta aagtttgttt tcgttcatac ttctccagtt ctgcatttcc
     4021 aagatttgct tttgtgattt ctatataata accgaaaacg cggttaaatc cgatttttaa
     4081 gtttttgatt cctgtttctt gacgctcttt tgcttcaagt tctgccaacc atttctttcc
     4141 gtttctcata gcagaacggt acatatcgag ctgctcattg aatccatctt tgatgatgtt
     4201 tccttccgtt atttgtaacg gcggatcatc ttggatagct tggtcgatca attggacgag
     4261 atggtccatc ggctgcattt ctgaaagcag actttgccat tgaccttgat cgattccttc
     4321 aatcaaccct ctgatcattg gcacttgttc taaagaattt ttcagctgga tcaagtctcg
     4381 cccattgaca ttaccgaaag ctactcgacc tgctaggcgt tccagatcat agactttcgt
     4441 taatgctgac tgcaaatcga tacgttcaaa atagctgtct aacaaagaag cgaccatttc
     4501 ttgtcttgct ttaatttgtt ttgcctggat aagcggacgg tctagccatt gttttagtaa
     4561 acggccaccc attgctgttt ttgtttcatc taacaaccat agaagagtgc cgtgcttttt
     4621 cccagtccga atagattggc ttaattctag gttgaatttt gaataataat ccattttcaa
     4681 aaaatgatct ggttggtatt ctactgcttt ttggatatga gctaaactcc gcttttgtgt
     4741 caccgataaa taagtcaata gtttgccggt tgcttctttt ttcagcggat cgactagttc
     4801 gcttgtcaaa aagctaaatt ccgcgttttc ttccatctca ttttgttgag aaaagactag
     4861 ccctaaacgc ccgcttagct gttcacgaag ggtatcagtg atctcactgc ctaatacgag
     4921 ttcttttgtc tgcaatgcag aggcttcatt taaaacagct tcttcatcat gcaaccgagc
     4981 cgtcttcaat tctcccgtac ttaaatcggc ataagcaaag ccaaattcat ttccttgact
     5041 aactacagct gttaaaaaat tgttgtcttt tgccgacaat cctttgctgt ccatgactgt
     5101 accaggagtg actagctgga tcacttcgcg cttgaccatc cctttagtcg tctttggatc
     5161 ttctacctgc tcgcaaatcg ctactttata gcctttttca atcaaggcat cgatatatcc
     5221 ttgtgctgca tggtgaggta caccgcacat aggaataggt tcttctgcat ttcgatttct
     5281 gctcgtcaac gttaattcca aaagctgtga cgctttgatc gcatcttcgt aaaatagttc
     5341 ataaaagtct cctaaacgat agaacaaaaa agcatcttgg tattgggcct tgatgcttag
     5401 atattgttcc atcataggag tatttttagt tttttgaggc atgtttttcc tcctctattt
     5461 gtccattgat ttcttcttgt atcatagttg ttaaatgatg aagcagatca tctgcttcca
     5521 acagctgttt tctataagct acaacaagcg gatgctgatc aaattcttgc atgaattgat
     5581 ccgcttgttt gattgcttct tgttctgctg ctggtttgtc atagtgggca aaatgaacag
     5641 catctttttg cgcttgtttg attttttctc tcaactcagc taaatactgg ttttgctgga
     5701 tcttttcttc aagttctttg tagcgaatga tcgtttcatt ctctcttaaa agttttgtca
     5761 gtttccttac ttctttttga atttccggct cctgctgcaa aaaactgcct ctctttctac
     5821 tccatgaacg gtcgatcatc gttgatctga atcagctgga ttttttttgc atgacctgtc
     5881 gtatcatcca attccaagat acagccgctt aaaatcgatc ggccttgttc cacaacttca
     5941 aaacgttttg gtaatgcggt cagaaacttt tcgattacag gttctctgcg catacccaaa
     6001 ataccatcat agggacctgt catcccaacg tctgtcagat acgctgttcc tcttggcaaa
     6061 attcgcgcat catttgtctg aacgtgtgta tgtgtaccga ccactgctga aactttccca
     6121 tctaaaaacc agcccattgc ttgtttttca cttgttgttt ctccatgaaa atctacaaaa
     6181 atgatcggcg tacgttttct ggcttcttct accagctcat tcattttacg aaaaggatca
     6241 tccaagtcca ccataaaaga acgagcctgc atgttgatca ctgccagttc gacttggttc
     6301 actttgacaa agaccatccc ttgtccaggc gtcccttccg gaaaatttgc cggacgaacc
     6361 atctttttag cttcatttac aaactcaaag attccgcggt tgtcccacgt atgattcccc
     6421 atcgtcacga catctacgcc gtcttgcaaa aattttttat agattttttc tgtgattcca
     6481 cgtccagaag cggcattctc accatttaca atcgttactt gcggacggta cttcttcttc
     6541 aatctaggaa gatattcagt aatcatctct cgtcccattg aacctactac atcaccaata
     6601 aacaataccc gcacactaaa ttcctcttct cttttaacat cactctatat tatagttgtt
     6661 ttcaataaga aagaaaaggt caaacaaaag gaactctttc ctggtatttc ttagttttga
     6721 aaaataaaat aaaaaaatgg gactatgaca aacatcttgt cacagtccca tttcccgaat
     6781 aaacgttgtt tcatctactt cttcagaaat aagccgtaat tttacaaaaa tttgagaagc
     6841 aattttcgta aatcccttct tatttcttga aactgaccac ttctgtcacg acctcttcct
     6901 taaacggtgt tcaaaagctt gcatctgcgg taataagccc agaaaaacga aaaataggag
     6961 gagctatttt tgtttggctg ggcttattac ttgatgcaga acagcttttt cacaacctct
     7021 tttaaatcac gcttaactct ttcaatgcct tccaaattcc atcctgatcg tttgtatcag
     7081 tgaccatctt agcttctgct tgtacttctg gcacagcatt ccccatagct atcccaattc
     7141 ctgacatgcg caacatttca cggtcgtttt gtccatcgcc aaagctgatg atgtcttcat
     7201 gagggatgcc tacttgttct gctaagtgca gaattgtcgc tgctttagaa ccatttttag
     7261 gtacgacatc tacactgttc tcatgccagc ggacaaatcg aattttatca tatgaagaga
     7321 acttcccatc atatgtttga tcgaaaaatg ctaacgcttg gtatatttct tgttcctctg
     7381 caaaaaactg atctaattca ggcaagacag caccgaaaga atgcatcgct tcttccatca
     7441 ccgttacatc attggaagaa gaacgcttga tattgtctaa gctgacaaaa gctgtatcga
     7501 tctgctcatg atgcgcttct tgaacgaacg catccaactg gtcgcgatct agtaaattct
     7561 tatatacttg ttcatggtct aaaaaaccgg ccgctccatt acataaaatg tagttcgtaa
     7621 aatcaagatc gcgaatgatc tcttgcgcat gaaatcggct ccgccctgta gcaatcgtga
     7681 ccagatgacc ggctttgcgt aatttttcca atgcttctct cgtgctatct aaagcttgtt
     7741 tgtttgaatc taacaacgtt ccatcaatat caaaagcaaa taatttgcgt ctcatctctt
     7801 gtcgtctcgc tttcttataa atacttactt gcctctcgaa cagctaactt actcaaatct
     7861 gttgtcagct gtctttttac ccaatcggca tcggtcttac tgtaatcgcg taatgcccaa
     7921 ccaattgctt tttggataaa gaattccttt gtttgacgat cttcgtctat tgctcggatc
     7981 aatagtcctg tgtctgtttg ctgtttataa cctaattgca acgtgattgc tattcgacgt
     8041 agccaaaaat cttccttttt ttcaaacaat tgaaagactt cttcacgctg ctctgggtag
     8101 gctttacacc atgtgctgta tacttttctc caagagtcga tgctgtccca ccattctttt
     8161 ttctgaacta gtggcaacaa tacgactaag tcttcaaatg taagccgttt gatgtttttc
     8221 tggactaaat cgatcgctac atactgatac tctctttcga tttgttcata ataataataa
     8281 acaaaagcta aaacttcaga aatagataaa tttttgctct catccagtaa cggttgttgt
     8341 aaaagagcgc gttccggctt aggtactcca gcaaaggaaa aaagattttt catgtacttt
     8401 tccatttgta acgctttttc ctcatttttt ggaaaaacta aaacttccat ggcacttccc
     8461 ctttaatgaa cgacttttta gtccacatag gatgattttg actgattcag ccaagcatat
     8521 cccagtccca ttactaaacc gccaccaata taatttcctg ccaaagcaaa aaacaaatta
     8581 tgtaacacat tgctgactgt cattccatcc gtctgtcctt gagaagcaaa gtaagcaagc
     8641 gtaaaggcag gaaaattcgc tatcacgtgt tcatatccta aaaaagcaaa aatgaaaatg
     8701 atgaatacga ctgtgatcac tttcccggca tcctctttca tccgcatgct gatcagtaca
     8761 gcggtattga caacgatatt cgcaaacatc ccttccacca aaatcgttgc tgcaggtttt
     8821 gctagtttta ctgctattga ttctaacata aagttgtcca acgttaaatc ttgaaatggc
     8881 gtagtcatag aaataagaaa tccacataat ataccgccaa tcatattgaa aaaaatacag
     8941 gcaaatagaa tcttggctgc cttacttaaa gcaagtctct gccgaaaaac acccacggtc
     9001 atataaagca tattcgatgt tcctaattca gcgttcatgt atagaatcat gaccagaccc
     9061 cagctgaaca taaatgcgta taaaatcttt cctagaccat gcgcgatatc ttcccccttc
     9121 atcgcgattg caaatgccac agccgttcct aaagtcaaaa acaaacaggc gagaaccgct
     9181 cgaacagcgt aacgtgaaaa gctgttttca aaaagattcg ccttcttttc aattgaagca
     9241 tcgattttct caaataatgg tgataccgga ttcattgatt ttctccttaa acttccttcg
     9301 cttttttcca tttgcacatg ccctctcatt ttataaagac agcctaggtt tgtaaagcgt
     9361 ttttatgtac taattatctt gacattcgtg gatgatctat catatactgt agtttgtgta
     9421 aaataatgca gcagaaaaat aataactcat ccctagtttg ttgccccgtc tcgggttcga
     9481 ttgtgtaacc gcggctgcaa aggtgaagat tgaagaacaa actatatgat tatgaatttt
     9541 tcaacggatt attttactcc aaaaacttat attcattgga ggaaatttta atgtctcgtt
     9601 atacaggacc atcatggaaa atttctcgtc gcttaggcat ctcattgtca ggcacaggaa
     9661 aagaattagc acgtcgccct tacgcaccag gtcaacatgg acctaacagc cgtggcaaac
     9721 aatcagaata cggcatgcaa ttaactgaaa aacaaaaact tcgtcacatg tacggaatga
     9781 acgaacgtca attccgtaac ttgttcgtaa aagctagcaa aatcaaagaa ggtaaacatg
     9841 gtgttaactt catgatctta ctagaacaac gtttagacaa tgttgtttac cgtttaggac
     9901 ttgcaacgac tcgtcgtcaa gcacgtcaat tagttaacca tggtcacatt ttagttgatg
     9961 gcaaacgcgt tgatatccct tcataccacg tagaagtggg tcaagtgatc tcagttcgtg
    10021 aaaaatcaca aaacatcgta acagtaaaag aagctgtaga agcaactgtt ggacgcccag
    10081 cattcgtaag ctttgacgct gaaaaattgg aaggttcatt gactcgttta ccagaacgtg
    10141 acgaactata tccagaaatc gacgaagctc ttgtcgttga atactacaac caaagcttat
    10201 aatttcaaaa gctttcttga aaaagaccgc tatctattgg atagctggtc tttttttata
    10261 ttttctattt acaaagtaat cactttgttt tacaatttcg ttaaccaaat aaaaatatta
    10321 agttaacgaa aagagggcat tatgaaccga acattgaaag aattaatact tgctgcggaa
    10381 tttgccgcga ttatttctgt cctttctcag atgaccattc cttttggctt ggttcctttg
    10441 acggggcaaa catttgctat tggattaaca gcaacttttc tcggaagaaa actaggattg
    10501 atatctgttg tagtttatct tttacttggc ttgattggtc ttcctgtatt ttcagggatg
    10561 actggaggta tagctgtttt atttgggcca actggcggat atttgattgg atttctctta
    10621 caaacgtgga taactggttg gatgatcgaa agaacggatg ctcattactt atatgcaatc
    10681 tttgccaatc taatgggctc tcttgctgca ttgatctgcg gaacgatctg gctgaaaata
    10741 agtggtgatc ttacttttac aactgcattt gccagtggac tactgccgtt tttactgccg
    10801 gaagcaatca aggcaactgg agcagcttat atcggtatct tgatgaaaaa acggctaaat
    10861 cgatttcttc atacaaatta atcaaaaaag cagcatggaa gaagactcaa agtctcttcc
    10921 acgctgccat attttaaatt aaacagatct ttattttgac aaatccactt ttttaccgat
    10981 ccaataaata attatcccac cgacaatcat tgatgccaat tctcctaaag caatcaccca
    11041 ccaattcaag aaaaaaggta gttgatataa aatcgttagc tgaccagcta ctgtaaacat
    11101 ggaaaaagca aatacgcatg ctgtcacagc cattttgatt ttcatgtttt tgatgcgact
    11161 agtgatttta tagttgataa gcaatacaag cagcgtgctg acgcttccta ctacaacatc
    11221 tatcagtcca agaggagaag ccatattagc caaagctact cccgctgtta ccgcgacgat
    11281 gtatcttttg ttatacaagg acagatagtt gaacatctcc gagagacgaa tctgaatcac
    11341 tccaaagcta aagacagaca aaaccagcgt cactgccaca tataaaccag tgactagcgc
    11401 catccgagtc acatctgtcg ttgtccagcg tttggctgaa cgggtattat ttttttccat
    11461 gttctatttc tcctttgcaa gataatttta tcttggtaat gactgcgacc aaaggatggg
    11521 aagaaaattc caactgaagt gccgcagaag gtagtgaaaa agctgccctg caacaagtaa
    11581 taagaatagt caaataaaaa tagctcctta tatttttcat ttggctggac ttgttaccgc
    11641 agttgcaagc ttttgaacac cgtttatatc aaaaggtcgt gaaaagccgc ttagtttcga
    11701 atattaacga ttattaaaaa gaattttttg aactggctgt tcggcatcga atgtcgggaa
    11761 cagtcatcga ctttctttta tcatcaaccg ttgtttctct ctttagtttt atcatacaga
    11821 agcttgaaaa aaacacaagg ttattttaac aaccatccgc catcgattgg gatgatcgtt
    11881 ccttgtatgt agcttgcttg aggagaggca agaaataacg tcagctcagc tacttcttcc
    11941 ggctgtgccc atcgcttgac tggtgtttca tttgcgaccc actcagccat ctcacctttc
    12001 ccttcaaaat cagccgcatt cattggtgtt tggatggctc ctggagcaat tccttttact
    12061 cggataccct gtcctgcgta atccagagcg agttgtttgg taaatccgac gattgcatgt
    12121 ttcgcacttg tataagcgat cccacctccg ccagcaacca aacccgcaat cgaagccatg
    12181 ttgatgattg ttccgctttt tcggcggatc atatcaggaa ggatgatttt tgtcagctga
    12241 tacatgcttt tcacattcgt ctcataaata agatcccaca gcgattcgtc agtttccatc
    12301 aatggtttgt aggcatccaa tatccccgat gtattcagta aaatcgtaac ttcaccaaaa
    12361 cgatcctggc atttcgatac cgcttgttgg agttctttca tttgtcgaac atcacctagt
    12421 gcgtaggcaa aagtttcagg aaatcgcaaa gagagttctt ccattttacc gaacttttga
    12481 tctaatccaa atacaaaagc atcttgttca agaaacgctc gggcttgcgc agcaccaatt
    12541 ccagaagcag ctcctgtgat aaatacaacc tgtttagcta gatctggata ctggtttttc
    12601 attctacgat cgcccaatca tctgctaaaa tatcacaaac tgtaggagcg aagctggaaa
    12661 aaccttcgtc acttgttttg atcaataagt aaggtgtcac tggacagcca tcaaattcat
    12721 catcggatac gagaacaaca taaagttcaa atccactcca tcctttacga ataatctttg
    12781 cacctttttt caattctggt aaaatttctt caaatgtcat ct
//
LOCUS       DAXWLG010000068        12310 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXWLG010000068 DAXWLG010000000
VERSION     DAXWLG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.068"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            33..1661
                     /gene="treC"
                     /locus_tag="ACGE4T_001537"
     CDS             33..1661
                     /gene="treC"
                     /locus_tag="ACGE4T_001537"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFF8386818.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(1721..2902)
                     /locus_tag="ACGE4T_001538"
     CDS             complement(1721..2902)
                     /locus_tag="ACGE4T_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8386819.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            3154..4677
                     /locus_tag="ACGE4T_001539"
     CDS             3154..4677
                     /locus_tag="ACGE4T_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8386820.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(4746..5473)
                     /locus_tag="ACGE4T_001540"
                     /pseudo
     CDS             complement(4746..5473)
                     /locus_tag="ACGE4T_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
     gene            complement(5486..6766)
                     /locus_tag="ACGE4T_001541"
     CDS             complement(5486..6766)
                     /locus_tag="ACGE4T_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8386821.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(6907..7509)
                     /locus_tag="ACGE4T_001542"
     CDS             complement(6907..7509)
                     /locus_tag="ACGE4T_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8386822.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7754..7960
                     /locus_tag="ACGE4T_001543"
     CDS             7754..7960
                     /locus_tag="ACGE4T_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386823.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            8272..8991
                     /locus_tag="ACGE4T_001544"
     CDS             8272..8991
                     /locus_tag="ACGE4T_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8386824.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(9072..9755)
                     /locus_tag="ACGE4T_001545"
     CDS             complement(9072..9755)
                     /locus_tag="ACGE4T_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFF8386825.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            9973..12159
                     /locus_tag="ACGE4T_001546"
     CDS             9973..12159
                     /locus_tag="ACGE4T_001546"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFF8386826.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         4447 a         2109 c         2379 g         3375 t
ORIGIN      
        1 gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc
       61 aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa
      121 tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta
      181 tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc
      241 ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga
      301 tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa
      361 aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg
      421 attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt
      481 atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag
      541 aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg
      601 atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca
      661 aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa
      721 cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa
      781 attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc
      841 atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag
      901 aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg
      961 ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc
     1021 agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc
     1081 cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg
     1141 actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag
     1201 aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc
     1261 aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag
     1321 gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct
     1381 attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc
     1441 atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc
     1501 agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat
     1561 tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg
     1621 agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc
     1681 gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt
     1741 cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt
     1801 tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa
     1861 aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa
     1921 tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa
     1981 gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg
     2041 caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca
     2101 acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc
     2161 agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt
     2221 gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg
     2281 ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac
     2341 cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg
     2401 cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag
     2461 aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac
     2521 tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt
     2581 caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa
     2641 cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca
     2701 aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc
     2761 cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga
     2821 taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg
     2881 ttgtatctgt ttcttttttc actctttttc ctcctttatc tataacttca ttatagacaa
     2941 aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata
     3001 taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa
     3061 attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc
     3121 aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc
     3181 atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt
     3241 aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc
     3301 gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc ccacgattat
     3361 gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat
     3421 gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat
     3481 acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat
     3541 gaccatgaac acggggaaga aggccataca catgagctag acccacatgt atggctagct
     3601 ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca
     3661 gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc
     3721 catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat
     3781 gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt
     3841 tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa
     3901 catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta
     3961 gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa
     4021 caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa
     4081 aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc
     4141 gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc agattgggca
     4201 ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa
     4261 tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag
     4321 ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt
     4381 aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac
     4441 gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct
     4501 tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat
     4561 ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca
     4621 ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag
     4681 gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt
     4741 tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt
     4801 gccaacgtga cttctctggt tttttattaa tcgaccgcac attttgtgta ttgataacat
     4861 aggatttatg acaacggata aaattccgat gcattttctc gatttctttg atcttctcat
     4921 agaattgtaa ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag
     4981 tagaaaaata ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt
     5041 tgaagatatc tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat
     5101 gctcgacatt ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga
     5161 tgttgctttt taagattttc atcgacattt ccgtatggga agtcacatag attatttttc
     5221 ctaaaggatc taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt
     5281 ctagttcgat atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga
     5341 gaagttcttc cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga
     5401 ttctattttt gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat
     5461 aaatgggaat catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac
     5521 gctggttttc aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca
     5581 agataaacaa gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt
     5641 tcaactggtt taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat
     5701 gattcttctt gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa
     5761 tcgcattgtc caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca
     5821 gttcgattgg ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga
     5881 gttttaatga cagtaaacta cggatctcag gaactttcat attatccaac atacttagat
     5941 tggccggaaa aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc
     6001 gtaatccttc taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt
     6061 aatcatgttt gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct
     6121 gttgcatcct gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc
     6181 aataataaac caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca
     6241 aagctaagac atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg
     6301 taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag
     6361 tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa
     6421 tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat
     6481 cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt
     6541 aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag
     6601 caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa
     6661 tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac
     6721 aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc
     6781 attgattttt accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt
     6841 ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt
     6901 aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt
     6961 aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta
     7021 agaagctgag aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt
     7081 taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata
     7141 gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt
     7201 caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag
     7261 acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga
     7321 aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac
     7381 cgctagtttt ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac
     7441 catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt
     7501 atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc
     7561 ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt
     7621 cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg
     7681 acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga
     7741 tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa
     7801 atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt
     7861 gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg
     7921 gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga
     7981 gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa
     8041 cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt
     8101 ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga
     8161 gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt
     8221 atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata
     8281 attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt
     8341 gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg
     8401 ctattttatt ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa
     8461 cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc
     8521 tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa
     8581 gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca
     8641 caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga
     8701 atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac
     8761 tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa
     8821 atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc
     8881 gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc
     8941 cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac
     9001 taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg
     9061 attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc
     9121 tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac
     9181 agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga
     9241 tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc
     9301 gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt
     9361 ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg
     9421 ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga
     9481 acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt
     9541 agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat
     9601 tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa
     9661 gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt
     9721 cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat
     9781 ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta
     9841 aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa gaaaaaatag
     9901 acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa agatgggaag
     9961 gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg
    10021 gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat cattgctacc
    10081 tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca agtaaaagaa
    10141 agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa tcgcttcaat
    10201 caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat catagcagga
    10261 gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa
    10321 caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc aaatgaacta
    10381 aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg aacattccat
    10441 agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa
    10501 ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt tttgcaacag
    10561 ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac gattgcttct
    10621 cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca gttagttaaa
    10681 gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg actgtatgga
    10741 ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt caatgatgta
    10801 ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat cggccaaaaa
    10861 tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg
    10921 aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga tgatcagagt
    10981 atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa agaatatcct
    11041 accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt ccaaggagca
    11101 aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat tccagcacaa
    11161 aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga aagtcagtgg
    11221 ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga atcaatcata
    11281 ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa aacgaatatt
    11341 ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa gtctttccaa
    11401 catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa gaaaaatcag
    11461 gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa aagtgaatgc
    11521 gatcaatact tttctcctaa atatcaaaat atagccgttg cagattatat tttgtcgata
    11581 agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa
    11641 acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct gccacgtata
    11701 gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa gcagcatgct
    11761 actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa agaattaaaa
    11821 acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca gagcattcac
    11881 aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga aggaagtttg
    11941 ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa agcagaacca
    12001 gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag agcaagaaat
    12061 cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt atcgagattt
    12121 atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga
    12181 agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa atttttgtga
    12241 aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg aaaagggagg
    12301 tgtgacaaga
//
LOCUS       DAXWLG010000069        12297 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXWLG010000069 DAXWLG010000000
VERSION     DAXWLG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.069"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(42..2117)
                     /gene="glyS"
                     /locus_tag="ACGE4T_001547"
     CDS             complement(42..2117)
                     /gene="glyS"
                     /locus_tag="ACGE4T_001547"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFF8386827.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2119..3036)
                     /gene="glyQ"
                     /locus_tag="ACGE4T_001548"
     CDS             complement(2119..3036)
                     /gene="glyQ"
                     /locus_tag="ACGE4T_001548"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFF8386828.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3420..4232)
                     /gene="recO"
                     /locus_tag="ACGE4T_001549"
     CDS             complement(3420..4232)
                     /gene="recO"
                     /locus_tag="ACGE4T_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFF8386829.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4375..5274)
                     /gene="era"
                     /locus_tag="ACGE4T_001550"
     CDS             complement(4375..5274)
                     /gene="era"
                     /locus_tag="ACGE4T_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFF8386830.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5289..5684)
                     /locus_tag="ACGE4T_001551"
     CDS             complement(5289..5684)
                     /locus_tag="ACGE4T_001551"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8386831.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5668..6144)
                     /gene="ybeY"
                     /locus_tag="ACGE4T_001552"
     CDS             complement(5668..6144)
                     /gene="ybeY"
                     /locus_tag="ACGE4T_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFF8386832.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6161..8353)
                     /locus_tag="ACGE4T_001553"
     CDS             complement(6161..8353)
                     /locus_tag="ACGE4T_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFF8386833.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8377..9348)
                     /locus_tag="ACGE4T_001554"
     CDS             complement(8377..9348)
                     /locus_tag="ACGE4T_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFF8386834.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10054..11433
                     /gene="brnQ"
                     /locus_tag="ACGE4T_001555"
     CDS             10054..11433
                     /gene="brnQ"
                     /locus_tag="ACGE4T_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8386835.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11587..12033)
                     /locus_tag="ACGE4T_001556"
     CDS             complement(11587..12033)
                     /locus_tag="ACGE4T_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFF8386836.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12060..12236)
                     /gene="rpsU"
                     /locus_tag="ACGE4T_001557"
     CDS             complement(12060..12236)
                     /gene="rpsU"
                     /locus_tag="ACGE4T_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFF8386837.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
BASE COUNT         3292 a         2588 c         2243 g         4174 t
ORIGIN      
        1 ctattttgtg cctgttctct ggtttattta catattagaa attattttgt gatgatttga
       61 tctaaacttg ctagtgacaa agccatggca gcaatttgac ttaattgcgt taatcggttc
      121 ttacgtactg ctggttcttc tgccatgacc atcgtctgat caaaataatt ttcgatcagt
      181 gggcgaaggc ttacaagctg atcataattt tcgttgatcg tttggtcagc aaagcttgtt
      241 ttcattgcct ctactgcttt gtgcagttct ttctccgctt cgttttcgaa cagttcaggg
      301 tctacttctg attgtttttc aagctccgct tttttcgcta agttcacgac acgtgtcaaa
      361 gcttccatcg atggtttgaa gtcttcatct gcaagatgct gcttgaacaa ttgtgctgct
      421 gcaaatactt ttgtcagatc tttttgttct gcattcaaaa cagcttcgat gacatcatga
      481 cggatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacctgt
      541 tgttgtcccg ctgacaagcc aatgccaaaa cgatccgtat ctttactgat aacttcacta
      601 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gattcgaacg
      661 acaccatatg cttgacggcg caaagcatat ggatcgtttg agccacttgg gatcatccca
      721 actgcaaaga aagaaaagat actatctaat ttatcagcaa tcgctaaaac agaacctact
      781 gtagtctcag gtaaatctcc ttcactggat ataggcatgt aatgttcacg gatcgctgtt
      841 gctacggctg gtttctcgcc tttaagcaga gcatattttt cgcccatgat cccttgtagt
      901 tcagggaatt ctcctaccat gttggttact agatcaaatt tatagatttc agcagcgcgt
      961 tgcagatcca tcaattcttc ctcagatagt cctacttgtt cgcctatgat gcgagcgatg
     1021 acttgtaccc gttgcatttt ctcatagatg ctaccgattt tttcatggaa agtaacagtt
     1081 tttaattttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
     1141 gcatcttcta aacgagcagt caatactttt tgattgcctc gtaccacatt ttccaatttt
     1201 acccgatcgc cattacgagc agaaataaaa tgaggaagca acataccttg atcgttccgt
     1261 acttcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagat
     1321 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
     1381 tcttctagta aatcttcatc tagatcaatg atccagtgat ttttttctgc taattcgttt
     1441 gcttgtgtga tgatcatttc ttttcttttt tgcggtgaag caataacaaa ttgttcattc
     1501 aatttttctt cgtattgttt tgcatgcgta aatgtcacat cttcgcctaa gaaacgatga
     1561 cctcttgaag ctctgtctgt tttgacatcc aagatctcaa acgggattat ttcgtcatct
     1621 aacaatgcca cgatccagtg aatagggcga atatattcaa aatcgtattc tgcccagtgc
     1681 atcgttactg ggaaagtgag gcttttaatg acatctgcta aacctgtcaa tacttctttt
     1741 gcttctttgc cgtgagtgta tttcgttaca tagacatatt ccacgccttt taattctcta
     1801 aaagtgatag catctgtcgt cagaccttgt ccgcggacaa atccttcagc tgctttcgtc
     1861 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcgctg
     1921 tctgcttgac gttctggaat gtttgttact ttgattgcta agcggcgcgg tgtagaaaac
     1981 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
     2041 aattgtttca tgcttggtgt gactacatga gcaggtattt cttccagccc gatttcaagt
     2101 aataagtctt ttgccatctt attcagcctc cttcacgttt tctggatttg ctgtgttttt
     2161 attcaataaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
     2221 agccatgttg cggatacggg aaagataacc tgcacgctct gttacggata ctgcaccgcg
     2281 agcatctaat aaattgaatg tatggctaca tttcaaaata taatcatacg ctggatgaac
     2341 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaaat tgcttagtag
     2401 caggtcttgg tcgcttactt caaaagaata tttagaatgt tcatattctg gttgtttgaa
     2461 gatctcacca tatttcacgc catcagccca ttccagatca taaacacttt caacttcttg
     2521 gatataagaa gcaagacgtt ctagtccata agtgatctca gaagtaactg gtttacaagg
     2581 tagtcctccg acttgctgga agtatgtgaa ttgtgtgatc tccatcccat caagccatac
     2641 ttcccagccc acaccggcac aacccatcga tgggttctcc cagttgtctt caacaaagcg
     2701 aatatcgtgt tctaatggat caattcctaa aagacgcaag ctttctaaat aaagctcttg
     2761 gatattttca ggtgaaggtt tcattacgac ttgaaattga tgatgctgat acaaacggtt
     2821 tgggttttct ccataacgcc cgtcagctgg tcggcgggat ggctctacat aagcagcatt
     2881 ccatggttcc ggtccgattg cccgtaagaa tgtatatggg ctcatcgtac ctgctccttt
     2941 ttctgtatca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
     3001 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttta
     3061 agcgttgctg tcaataagaa tacaggaaag aaaacgacaa aaagtcccta tgtctgatta
     3121 cagacatagg gacgaataat ttcgcggttc caccctactt ccgaataaag attcggcagc
     3181 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc ttccactctc
     3241 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
     3301 agttttctgg tttgtataat agatcctctc agtgaacgat ctatttaatc tacggttaat
     3361 aatagacgga agtccctgta tttgtcaaat agttttgatt tttttgttgt atctttcatt
     3421 tattcttctt tttcaggtat ctctttttca ggcatatttt tctctggcgt ctttaatacg
     3481 tgtgcccaag acttcatttg atcaatgaac tttttgcttt ttagatgaat accgacatat
     3541 tcatcataca actggtcaat gaggtcacgg attgcctgct tcgtttcttc tttcaagtgg
     3601 attccactga tcttatcata agaaatagcc gaaaacattc gaacgaaata aacagcgcga
     3661 ggatctgcat gataacggtg ttcgtccatt ggccagtgtt tctcgcatat cacaccgcca
     3721 tatttggaag aatagtcaaa ttttcctttt gtttccccgc atacgacaca gtgcgtccaa
     3781 attggcgcga ttccgaagcg ttgcagtatt tgcacttcaa agatattcgt gatgatttct
     3841 gcatcatctc cttggtccat ccgctgcaga gcttgttgcg taaattggaa taaatgcggg
     3901 tcatattgat gatcttcgat tgctgcatct actagattta gcaaatacgt accataaccg
     3961 ctcaaaaaga tatcttcttg gatgttccga aacgcatgga cgtctttgac actgcttaaa
     4021 aacgacaaac cttcacttcg aaaattaccg atatagactg cttcggtaaa ggggagcaaa
     4081 gccggagcta atggctgatt ttgccgatgg attcctttga tataaaacat cattttgcca
     4141 gaagattccg taaagatttt gactaattta tctttttctt tgtaattacg gctagataaa
     4201 atcaagcctt tcgattctgc aagctggctc atattctccc tcctaaaaaa taaatctgga
     4261 aaagcagatt ctgccgatcc agattattta tgtttcgatc ttccgctgtt tttctcaacg
     4321 tacggatttt tatccagttt atttctatac ctaacctata agaagtccag aatatcagta
     4381 ctcgtctttt cggtagccga agtcttgcag atagactttt ttgtctcgcc agtctttttg
     4441 gactttgacc caaagttcta gaaatacttt gtctcctaaa agattttcga tatcctttct
     4501 tgctttcgtt cctacttgct tcagcatttt tccgcctttg ccgataatga ttcctttttg
     4561 gctatctctt tcgacgatga tcgtggcctg gatatggatt ttatcattct cgtttcgctt
     4621 catcgaatca acaacgactg cgacagaatg aggcacttcg tctcgtgtta aaaacaagac
     4681 tttctcacgg atcaattcag aaacgatgaa gtattcagga tgatcagtca cttgatcatc
     4741 tggaaaatat tgtgggccct ctggcatttc actgactaat acatccatta agcgttcgac
     4801 attgtttcct tctgtagcag agatcggaat cacttgtgca aaaggcatct gcgtcgaata
     4861 atcttcgatg attcccagca actcgtctgg atggatctta tcgattttat tgataatcag
     4921 ataaacagga gaatgcacgt tttttagacg ttcaataata aaatcgtctc cccggccacg
     4981 tttttgatca gcactgatca taaataagac tgcatccact tctttcaacg cactgtaagc
     5041 tgcttctacc ataaaatctc ctagacgatg ctttggttta tggattcctg gtgtatcgat
     5101 aaaaatcagc tgagcttctg gtgtagtata tactccttgg attttatttc tagttgtctg
     5161 tgccttgtca ctcataatgg caattttttg acccacgatt cggttcaata atgtggattt
     5221 tcctacattc gggcggccga cgattgcgac aaaccctgat ttatgttctg gcatatacgt
     5281 tctccttttt aattaaacaa caaatgatat attttaggga caaataaaat caacccaatg
     5341 atgacagcaa aacaagcggt cactaaaact gctccagctg ccatatcttt gatttttttc
     5401 ccaatcggat gaaaatgaaa atccgtaaac atgtccacta cattttcaaa aacagtattg
     5461 atgatctcta ctatccaaac gagaaaaaca gctaaaagca gccataacca ctctatccga
     5521 tcaagctgga aaatcacgcc cataaataaa gcaaggatac cgaacgcaac atgtttcctc
     5581 atattgcgtt cttcctcaaa aacggttttg actccttgga cagcaaattc cacagacatc
     5641 aggaaatgtt tgtttttctc tacgttttta tcttttgagt ccataggcat ccaagatctc
     5701 tttctgtaaa ccaaacattt ctttctcatc ttctggtgtc atatggtcat acccgttgat
     5761 atgcaagaat ccatgtaccg ctaaaaagcc aagttcccga tcatatgaat ggccgtaagc
     5821 ttctgcttgt tcagcagcac gttcaataga gatcatgatg tctcctagta cacgaggtaa
     5881 tggattctct ccatcttcaa aaatcactgg gatttcgtct tcgccttctt cttccagagc
     5941 aaaactgatg acgtctgtcg gtgcatcttt tccacggtaa tcacgattga ttacttggat
     6001 tgccgcattg tccatgaaag taacagacat ttctgtttcc tctggcagct ttaaataaga
     6061 tgctgcaaat tgcagcaagt catcgatttc tttcattttc tcttctgaga cttggtttgt
     6121 ttcatccata aaggtaatat ccattataca tcgcttcttt ctattcttct tgttcttcct
     6181 ttatcttctc aacttctgtt ttcattttag gatatttgat ccgagaatgg aaggtgctac
     6241 ttaaaccgct gattaatgat ttttccactt ttcggatttc tttcaaggtc agtccactat
     6301 cgtctaattg accatcggaa atacgctctt cgataagatt atggacaaaa ctttcgattt
     6361 tttcgctcgt cggatgatcc attgcccgta ctgcagcctc acaactgtca gcaatattga
     6421 cgatccctgc ttcacgactt tgcggttttg gtccaggata acggaattgc tcttctgtta
     6481 cttccggatt gcgttctttc gctttgatgt agaaataacg cattagcgtg gttccatgat
     6541 gctgtttgca aatatcgatc accatttgcg gcatattata atcttccaat attttggctc
     6601 catctgtcac atgtccaaag ataatatgtt tactgtcttc tggcaataag aaattgtgcg
     6661 ggttttccgc tcctgaaggc aagttttcaa cgaaaaagct agcatgtttg atcttgccga
     6721 tatcatgata atagcaagct actcgagtta gcaaagaacg tccgccgatt tctgctacag
     6781 cgttagcact tagattagct accatcatac tatgatgata agtacctggc gcttcttcca
     6841 gcaactgttt caataaagga tgatttggat tgctgagttc atttaataca attacactgt
     6901 catcattgac catcaattca atatatgggt gtagaccgat cgtcagcaag aatgagaaga
     6961 gtgcaccagt gaacccgcag acgagcatta accaagtcgt tgcatcacca aagctcattc
     7021 cttgatagac gatcaaaatg acatccatca tcacaggaaa aataatgacc cacatggcag
     7081 cagataatcc ttgttctgag atccgtttgc gtctgacaat cgttgccatg agcccagaga
     7141 aaacataaga catcaagata atcgttagaa agttcgtgcc aatcgaatca taaaaaatga
     7201 aaagtgcgga tactgcttgg aaaataccag ccatgatccc tgctcttcga ttcaagaaaa
     7261 agcttaaaac aagtggtgca aaagcagcgg gataaaacag cgggatataa gtagcttgtt
     7321 ccgtttggaa taattggaaa aatttcatca gcactatgct gactgacata gctgtcacat
     7381 agaaaagcac gtattttgtt ctttctcctg cattttggaa ttgaagtgaa ttataaatca
     7441 gtacgccgat ttgaagcaaa acagctaaaa ccatggcaat tagcggaaaa atcgaagttg
     7501 tttgattagt cagtcctagt aattccaatt ttttcatagc tgaagcatcg atttgattac
     7561 cttcacgaac aatgatttct ccttggaaga tcatcactgg ttgtacagct tctcgtgcgg
     7621 attgtttcaa ttcttctgtc tttttctcat tcaatacgtc attcactacg ataccttgat
     7681 ccactaagta gcgaatcatt tgttgttgtg ctggtgtgac attcaaatat tggatttgct
     7741 cttctgcttc ttgtttaaaa gcttccaatt cactttcacg aacttgtttg ctcatctgtt
     7801 cgtcaatgag cttcagactc tcatccctca cagtatgaat ctcagtttca gtcatttcaa
     7861 atatcgtcga ataaaaattg ttcggtagtt tttgataaaa cacaacattt tctgcattca
     7921 cattttcaaa atttttcttc agcgcggcga tccgctcttc tgtagtaggc tttgggatat
     7981 tttcatcttc ctttgcctta tcaatctttt cgtcataaga tttgttgatg gcatcattcg
     8041 tcttatcgat caattcaaat agttgcttga tccgattgtt ctggtcatct gcaagatctt
     8101 tttggtaagt gtattctgga gtgactgctt ctgctgccag ttttcttttt tgttcggttt
     8161 cttccgtgtt ctcgatcgtt ttgttcgcac ggatactttc ttctgctact tggccttctc
     8221 tgaaatcatt ggccttttgt ttgacactac taaatgtaac gccgcagata atgataaaaa
     8281 acaagaacag caggaaaggc aatgctgcct ttccaagttt ttcttgtagt ttctgcagtg
     8341 ttttattcaa catgtgctta tcctcctaag caaagcttat tcttgatgga gatctgcctt
     8401 ttcataagct ttgatgattt cggcaactac agggtgccga acaacgtcac ttgcttcaaa
     8461 attaacaaaa tcgattttct tgatttgctt taatgttcgt tctgcatgaa caagaccact
     8521 tgtcgtccct cgaggcaagt ctatctgact tgtatcccca ttgacaatca tttttgattg
     8581 ataaccgaga cgggtcaaga acattttcat ctgagcgact gtcgtgtttt gtgcttcgtc
     8641 caagataacg aatgcatcat ccaatgttcg cccccgcatg taagctaaag gtgcgatttc
     8701 gattacgcct cgttccatca atcggttcgt atgatccaat ccaaaaattt ggtataaagc
     8761 atcataaaca ggccgcagat aaggatccac tttttctttt agatccccgg gaagaaagcc
     8821 tagattttca cctgcttcca cagcaggccg cgtcaaaatg attttttgca cttcaccttt
     8881 tttcaaggca gcgattgcta atactactgc aagaaaagtt tttcctgtac cggctggtcc
     8941 tacgccaaat acgatatcat gtttggctac cgattcgaca tatttctttt ggccgctgtt
     9001 ttttacccgt ataggttttc cattacggtc tttgacgatc tcttcctcat acatctcaac
     9061 aaaataatct aatgtacctt tttgccccat tttcaatgcc gtcacaacat cgggcgtact
     9121 tacgtggatt cctcgtttga tcaattcctg taatgtccga atgactgagg cagccaagga
     9181 aatctgggat tgctcaccaa tgatctgaat gatttctcca cgtgtatgga tggttgtttc
     9241 cgttgtttcc tcaatcagtt ttatatgctt gtcgtgtgca cctaaaagca tgctgacatc
     9301 atcagcagcc gtcaatctaa tctctaaaga actagactct tccgtcaaaa atagacatcc
     9361 cctttttttc gattcattct ttcctatttt accatactca gaagaaacta tatagcaaat
     9421 aagaaaaggt tctaaaagag ttgcttaacc ataagtacca agaaacgtta aaaaactagc
     9481 cgcttccatt ctctaaaggt ctcgtttgaa taaaaaaagc tgatcatgta agctatgcat
     9541 aagatagagg acaagtattc cgtctaactt agcaagtatt ctctgggtat ttttcttgtt
     9601 tcaaaaaaag aaaacaaaat atttgaaata ttcttgcatt tcaaaaaaaa caggtgtatt
     9661 ataacgtcaa caaataaata tacagcaaag cgtcgaggaa aacagtaggc tattcagtga
     9721 tttattccca gtgagtcatg taagtgagaa atgacaataa atcgaataga cgaacatcat
     9781 ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc tccattatca ggctagcgga
     9841 taactttatc gaaaagcgtc cgtgaatgaa gaagcttttt taagcgaaaa aaggtggtac
     9901 cgttctagaa tctagagccc ttttcccaag tggaaaaggg ctttttttat attttaaggt
     9961 ggtggttttc atggaatctg atattagtgg tatttctagt ttctgcaacg tttattttgt
    10021 tactcaaaat gataattatt ggaggaaaaa tttatggaag aaacaaaaac ttattcatgg
    10081 aagcaattat tgatggtcag ttcacttatc ttcgggatgt tcttcgggtc aggtaatctg
    10141 atctttcctg tacacttagg tcaaatggct ggatcaaact ggatcagcgc tggtgtagga
    10201 tttgccatct caggcgctct tttcccatta ttagctatct tagctgtagt agtgacaaaa
    10261 agtgatggtc tgtatgactt agctaaacct gtcggaaaat ggtatgctgc cttgttcttg
    10321 atccttgttc atttaacgat cggaccattc tttggtacac cgcggacagc agcaactgct
    10381 tttgaaatgg ctgctaaacc ctttttgcct gaaaaatatg attcattagg tatgttttta
    10441 ttctctgctc ttttcttttt acttgcttat ttccttactg tacatccaac acgtttggtc
    10501 aaatatgtcg gaaagtattt aaactccttg tttcttgcat tattagctat catttttgtc
    10561 gtttctttat tccaacccat ggggaatcta aaccaatctg ctgcagattc ttatcagacg
    10621 aacgctttga caaatggcgt tttagaagga tacaacacgg ttgatgcagt agctttactt
    10681 gctttaagtg tcacttttgt ccatgcagtt cgtgatcttg gttttaaaga taaaaaagtc
    10741 acagaattaa cagcaaaagc tggtagcctg agcgttgcgt tagaagtcat gatttatttt
    10801 ggtctggtcc ttctcggtgc gatgagtttg aataagatgt ctttatcaga aaatggcgga
    10861 actgcacttt ctcagatcac taatttttat ttaggtaact tcggtgctag cttcttaggg
    10921 gtaatggtca cattaggtgt cttcactact gctatgggac tcgttgtttc ttttgcacaa
    10981 gatttccata aactgttccc taaagtcagt tatatgacat ggctgcggtt aacgactttt
    11041 gtctcttttg tcgttgcgaa tgccggattg gataatatca tccaatggtc tttaccagta
    11101 ttgatgcttt tgtatccatt gtcacttgcg ttaatcttac tttccttaac agcaaaattc
    11161 ttccaaaaga gtccatttgt ctatcaagta acgatgttct tttctgccgt tccagccgta
    11221 ttggatatgt tggcaaattc gcctgcatta gtgagtcagc aaactgtcgt agcaagctta
    11281 ctaggatttt atcatcatta tgttcctttt gctgcattag gtcttggctg ggtcgtccca
    11341 acgttgcttg gttacgctgg cagcttgctc ttttattatg tatatcgcgt atcaggatat
    11401 aaacaagaag caagtgagtt accagaagaa taaccaaatc aaacacaatt taacataaga
    11461 gatattaaac aggaaaaaaa cagctagaag aaagaataag attctttctt ctagctgttt
    11521 tttagttctt tttttacgaa cacaaaaaac tgcggataat cactgtccgc agtttaaatg
    11581 ataaaattaa gattgtaata gttctttcac aatcgcattc acttggtttc catctgcttt
    11641 tcctttgact ttaggcataa ttgcacccat aactttgcca aattctttca ttgatgaagc
    11701 accagtttga gtgattgctt cttgaacaag ttgtcgaatt tcttcgtcgg aaagttgttc
    11761 cggcatatat ttttcgacta ttacaatttc actttttact ttttcagcta aatcgtcacg
    11821 tcctgcttct tcaaactcat gcaaagaatc acgtcgctgt ttcatttcac gagacaacac
    11881 cgttagttct tcttcttctg tcaagtcgtg accttcttta atctgctcgt tttgtataga
    11941 tgacttgatc attcggatga cttgtaaaga ttccttatct ttcgctttca tcgctgcttt
    12001 catgtcatcg tttaatctac ttagtagtga cacactacca actccaattc atcacagaac
    12061 tagaattttt tgcgttttct agctgcttca gattttttct tgcgtcttac acttggtttt
    12121 tcgtagaatt cacgtttacg agattcttgt aaagtacccg cttttgacac ggaacgtttg
    12181 aagcgacgaa gagcatcgtc aagtgattcg tttttgcgaa cgactgtttt tgacatgtta
    12241 attccctccc tccgagctta aaaagtaacc gtaattgtta ttgaaatata tctcaaa
//
LOCUS       DAXWLG010000070        12052 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXWLG010000070 DAXWLG010000000
VERSION     DAXWLG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.070"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(349..930)
                     /locus_tag="ACGE4T_001558"
     CDS             complement(349..930)
                     /locus_tag="ACGE4T_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8386838.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1113..1739)
                     /locus_tag="ACGE4T_001559"
     CDS             complement(1113..1739)
                     /locus_tag="ACGE4T_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8386839.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1761..3092)
                     /locus_tag="ACGE4T_001560"
     CDS             complement(1761..3092)
                     /locus_tag="ACGE4T_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFF8386840.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3247..3648)
                     /locus_tag="ACGE4T_001561"
     CDS             complement(3247..3648)
                     /locus_tag="ACGE4T_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8386841.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3734..3940)
                     /locus_tag="ACGE4T_001562"
     CDS             complement(3734..3940)
                     /locus_tag="ACGE4T_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386842.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(4035..5537)
                     /locus_tag="ACGE4T_001563"
     CDS             complement(4035..5537)
                     /locus_tag="ACGE4T_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFF8386843.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5555..6010)
                     /gene="argR"
                     /locus_tag="ACGE4T_001564"
     CDS             complement(5555..6010)
                     /gene="argR"
                     /locus_tag="ACGE4T_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8386844.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(6108..6725)
                     /locus_tag="ACGE4T_001565"
     CDS             complement(6108..6725)
                     /locus_tag="ACGE4T_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8386845.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6946..8319
                     /locus_tag="ACGE4T_001566"
     CDS             6946..8319
                     /locus_tag="ACGE4T_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8386846.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8394..9506
                     /locus_tag="ACGE4T_001567"
     CDS             8394..9506
                     /locus_tag="ACGE4T_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8386847.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9578..11215)
                     /locus_tag="ACGE4T_001568"
     CDS             complement(9578..11215)
                     /locus_tag="ACGE4T_001568"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFF8386848.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11383..11991)
                     /locus_tag="ACGE4T_001569"
     CDS             complement(11383..11991)
                     /locus_tag="ACGE4T_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8386849.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3184 a         2547 c         2174 g         4147 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagca gctattttta tttggctgtt
       61 cttattactt gatgcagggc agctttttca caacctctcc actaaacgat gttcaatcag
      121 ctaaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt
      181 tctctaatac tcaggtcaaa gcgctgattt cacaacctct aatacacggt gttccagaag
      241 tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat
      301 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt aaagaacctt
      361 ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc
      421 ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg ctttggcttg
      481 acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat tttgtttcgt
      541 atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt catccactgt
      601 actcaactca ataatcaaat tacctacagg acagccatga taagtgatct gtgtctcatg
      661 atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt ttccagcatt
      721 taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga tcaccgctag
      781 acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg tatcagaagc
      841 tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa gatctttggt
      901 cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt tctcatagtc
      961 ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa gactgtgcca
     1021 aattatctga cacagccctc actataaaac tgttctcata tagctagatg ttcattgatt
     1081 caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa tacttcctct
     1141 gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc cattgcttct
     1201 tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact ctctaccaat
     1261 tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat tactgtgtcg
     1321 attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa gattttatga
     1381 ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat aaaatcggct
     1441 cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg ctgctcaagc
     1501 atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta aaaataatga
     1561 ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc aatcatattt
     1621 tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc gcctgctggt
     1681 ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt attgaacatg
     1741 gtggattctc cattcgttat ttattttaat acatcattta gagcttcact cattgtcgga
     1801 tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat agctagcgaa
     1861 atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag aatcaagtca
     1921 gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac ttgtgcttta
     1981 gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc tttggcttgt
     2041 ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta aggcacagct
     2101 ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat gattcgataa
     2161 tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat cgcccaaatg
     2221 ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc tgttagttta
     2281 acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt tgcagccaaa
     2341 acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac ggcagctgtt
     2401 gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat tcccgcattt
     2461 gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag aaattcagaa
     2521 tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc aaattccaag
     2581 ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg atccatcgct
     2641 tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa aatgattggt
     2701 tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc accattattc
     2761 gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt
     2821 gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa tttttctttc
     2881 cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa tgatttagaa
     2941 ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt ttcaacgact
     3001 aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa
     3061 ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt ttacctaccg
     3121 attggtacaa aaatatgata accgataatt gtctattctc caaatcatct gctttatttg
     3181 tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc gatttccttt
     3241 attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt tggagtaagg
     3301 gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga tgccatcgat
     3361 atgcacttct aatactacac cgatctgata gccgccatca tctaaattat aaagagagac
     3421 acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc
     3481 cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg ttccttctgc
     3541 atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct cgacttctcc
     3601 attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg tactgctcct
     3661 ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa ggcgctttct
     3721 acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt ccaaaaaaca
     3781 aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata gcaatctttt
     3841 ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag ttgaccccat
     3901 aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc ttttgctggt
     3961 ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc aactagcctt
     4021 tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg tccgaataat
     4081 tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc tatctgtaat
     4141 actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc gtatggctct
     4201 tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc tttttcttct
     4261 tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg
     4321 aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt gatttgttca
     4381 tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt tgcgatgaca
     4441 tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg
     4501 gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat tacatttcga
     4561 tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct ttgtagatga
     4621 gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa tacgattgta
     4681 cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact gacaactgca
     4741 gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc taaaacagcc
     4801 tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt aggaaatcgg
     4861 ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt caatactttt
     4921 tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca accatttagt
     4981 ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc ttgcgcataa
     5041 ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat tttttgctgc
     5101 tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt aaagaatttt
     5161 attccattat aataataggg aagatggcta gcagtcagca taaccgcagc atcgcacgag
     5221 aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc gaaatcgatg
     5281 acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc tggtccgctt
     5341 atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt tgatttcttc
     5401 tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag
     5461 tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc attttgcagc
     5521 gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt tccagctttt
     5581 ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta tctacaccgg
     5641 ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt accacatcgg
     5701 caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata cgcaaaacct
     5761 cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc tggttaaata
     5821 atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt tctcgaatgt
     5881 ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct
     5941 cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt
     6001 tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt aatatttctt
     6061 catattttgg tattttttac ttttattttt tgttttcatt caggttgcta acctttttct
     6121 attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg atcgctgatg
     6181 taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat gaatgcttct
     6241 aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat ttgggcacaa
     6301 agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg aagtttgcta
     6361 gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg tcggatcatc
     6421 aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt ttcttgcagt
     6481 ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat cagccaattt
     6541 tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac tcttcgttta
     6601 aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt tttaggaacc
     6661 tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca ttcaattttt
     6721 tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc tctttttgag
     6781 tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag tgaagtaaat
     6841 aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta tttttataat
     6901 agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa
     6961 cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc gggcgtattg
     7021 atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga tttaacgact
     7081 gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat tgttccgctg
     7141 tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc aaatctgctt
     7201 ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct attgtttggt
     7261 cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca tattatcttg
     7321 acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt gacaacggcc
     7381 attgctccag caattggccc cacttatgga ggcattatga cttcacattt tacatggaat
     7441 catatcttcc tgtttcttgt gcccgttttg attctttctt tgattatcgg ctggtttgct
     7501 attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt tttaggaatt
     7561 tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt ttccagctgg
     7621 attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag taaaaaagtg
     7681 gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt gtttttgatt
     7741 ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc gaatttcctt
     7801 cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc cggcgcagcg
     7861 gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg tccgaagaag
     7921 ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt cctactcata
     7981 ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat cggtatcgga
     8041 ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga actacaagga
     8101 gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc tacttcatta
     8161 gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga agtgtctact
     8221 acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt aagctttgct
     8281 cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt
     8341 gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa
     8401 aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac ttagatacat
     8461 caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa gctagcgctg
     8521 ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag
     8581 acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa acgatttacg
     8641 cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg gctgttcttg
     8701 ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt tcacctgaac
     8761 agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc agtggtggtg
     8821 gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca caaaaacaaa
     8881 tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg ctcatcaagg
     8941 gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg gcacttaagc
     9001 ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg cagctcttag
     9061 catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt gacggagaag
     9121 ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat gaaaacgatc
     9181 aaggctggga tttggctcct actagcgaag cttataaggg attgactaag agtacagaac
     9241 agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt gtaggtaccc
     9301 gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat ccaagcttac
     9361 tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc ggctatctat
     9421 tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag gacatctcca
     9481 cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga tcccgagcga
     9541 tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat
     9601 tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga gcttcaatga
     9661 atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta tcctcaaaag
     9721 aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca aacgacttga
     9781 attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca ggtttgattt
     9841 ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt ttcaaaaaca
     9901 actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga ttagcaaatg
     9961 gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg tctgatcgtt
    10021 ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc ttgataattg
    10081 gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg ctaagatgat
    10141 cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg tttcgtatat
    10201 ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca ttctgacaag
    10261 taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta tcaaagtaag
    10321 aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca
    10381 agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat tttccagaac
    10441 cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat tgtttcatcc
    10501 caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg
    10561 ctttttttaa ttctccaaag taacgtaatc ctagcacaac cgctgtatta tggagagcat
    10621 caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca caaaagatat
    10681 aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc ttcgaattcg
    10741 ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct ggaatcagca
    10801 aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta tggtaaaact
    10861 ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg ctttgttcct
    10921 tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg cttccatcat
    10981 tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt gagattgtta
    11041 aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc ttcctatcaa
    11101 aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga atactggata
    11161 acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta ctcatggctc
    11221 ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga gtttttattt
    11281 gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac cgagaacttg
    11341 aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc agccgtttgc
    11401 taaccagaat gttttagcag catcccatga accataacga gatgttacat attgttctgc
    11461 tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat ctaattgata
    11521 acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt tttgtgcgat
    11581 ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag caggagctac
    11641 ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt cagttgtaga
    11701 tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg aagttgggat
    11761 tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat
    11821 tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata atgaatcacc
    11881 tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa acattgcagt
    11941 agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca tattaaactc
    12001 ctttttttct ccgtttttta ttctttcgac gctattaact ttatcattta ga
//
LOCUS       DAXWLG010000071        12012 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXWLG010000071 DAXWLG010000000
VERSION     DAXWLG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.071"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            40..411
                     /locus_tag="ACGE4T_001570"
     CDS             40..411
                     /locus_tag="ACGE4T_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386850.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            496..696
                     /locus_tag="ACGE4T_001571"
     CDS             496..696
                     /locus_tag="ACGE4T_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386851.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            830..2056
                     /locus_tag="ACGE4T_001572"
     CDS             830..2056
                     /locus_tag="ACGE4T_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386852.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2653..3048
                     /locus_tag="ACGE4T_001573"
                     /pseudo
     CDS             2653..3048
                     /locus_tag="ACGE4T_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            3126..4165
                     /locus_tag="ACGE4T_001574"
     CDS             join(3126..3623,3626..4165)
                     /locus_tag="ACGE4T_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFF8386853.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            4178..5116
                     /locus_tag="ACGE4T_001575"
                     /pseudo
     CDS             4178..5116
                     /locus_tag="ACGE4T_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            5109..5456
                     /locus_tag="ACGE4T_001576"
     CDS             5109..5456
                     /locus_tag="ACGE4T_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386854.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            5453..5635
                     /locus_tag="ACGE4T_001577"
     CDS             5453..5635
                     /locus_tag="ACGE4T_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386855.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
     gene            5771..6628
                     /locus_tag="ACGE4T_001578"
     CDS             5771..6628
                     /locus_tag="ACGE4T_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8386856.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            6648..7208
                     /locus_tag="ACGE4T_001579"
     CDS             6648..7208
                     /locus_tag="ACGE4T_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFF8386857.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            8097..8864
                     /locus_tag="ACGE4T_001580"
     CDS             8097..8864
                     /locus_tag="ACGE4T_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFF8386858.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            8890..9435
                     /locus_tag="ACGE4T_001581"
     CDS             8890..9435
                     /locus_tag="ACGE4T_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386859.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYVHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            9436..10002
                     /locus_tag="ACGE4T_001582"
     CDS             9436..10002
                     /locus_tag="ACGE4T_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386860.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            10017..10265
                     /locus_tag="ACGE4T_001583"
     CDS             10017..10265
                     /locus_tag="ACGE4T_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386861.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANGFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            10285..10524
                     /locus_tag="ACGE4T_001584"
     CDS             10285..10524
                     /locus_tag="ACGE4T_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386862.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            10895..11176
                     /locus_tag="ACGE4T_001585"
     CDS             10895..11176
                     /locus_tag="ACGE4T_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFF8386863.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            11166..11507
                     /locus_tag="ACGE4T_001586"
     CDS             11166..11507
                     /locus_tag="ACGE4T_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8386864.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKTGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         4481 a         1675 c         2389 g         3467 t
ORIGIN      
        1 ataaatagga cacaaaattt ggaaagtagg taagtcacta tgagagaact aactttatct
       61 tctaaggaat tagaaaagaa aactgcttta ttaaacgaaa tatttagtgc tgattgtata
      121 atccaaagtt taaaagaaag ttcattttca aaactaacag atcaggaaaa aatttcttat
      181 atgctaagtt tagataatct aggaactaaa tatgatgaat cagcaagtag cgataagctt
      241 ttgattgaat tagtatcaag tcttagtagt tttggaaaaa tgaaaagatt taatgtgata
      301 ttagaaattt ttgcttggtt aatcccttca tttatttggt ttttttatat tttcatagtt
      361 gaatctgagt taatagccaa ggtctctctt ggactgttaa ttccgatata aataggttca
      421 gcaatttact ttaataactt gtatacacct attcaaataa aaaagcagtt attacatgaa
      481 ggattaagtg gtaatatgtt aaaaagaatt gcatgggata aagataataa aacagatatt
      541 gagcttaaag aatataaata caattatttc tcgagatgta tagatagaaa gttactgttt
      601 aaaaaagaag gattagaatt tagttttgat gaagatgaat ttgaaaaatt agtgatggac
      661 tggcgtaaga atttactgga tacggtacaa ttctaaaagc aattgaggtc gaaattccga
      721 tacttgaatc atgatctata ttatcgtgtt atgagtgaaa tttctaataa agataagata
      781 tttcatttgg acagtcatgt gaatatgagc ttaacaatta aacgtattta tgatctttta
      841 tatgtttata aacattaatg aggtgctaaa attgacaaca ataactatga caaaaaaaat
      901 agaagtatat gttgatggat cacaagaaga aagggattcc ttttttgatt ttttttggaa
      961 aaatagtaga gaatatgtaa aagcgttgaa tctggtatat aaacagctaa tgtttgaaga
     1021 gttgataaaa gatgcaatta aagaaatgga tcaaggttat gctgagagag aagaaagtat
     1081 tcaaaatcgg attgaaaagt tagcatatac tgatccaata gatcaaaaaa aacttgataa
     1141 agcaaaagct gaattatcta aattgagaaa tgaaaaaaat aaagaagcga gagaaatatt
     1201 aacgcaagcg atagggttga agagtcaaac gagaacacga gatgtagcaa aaaaattaga
     1261 ctttgacttt ggagattttg ttgattccgc aaatatgaaa gctagtaaag attttggcaa
     1321 tgattttatc ggaattgtga ccggacaaag aacgcctaga atatataaaa atagaaacaa
     1381 atatggatta ttacctttta gaggtcgaga tattaaaatc cataaagatg ataagtttta
     1441 tatttattta cctaaaggct ttaggtttaa agtgaattta ggtagcaagc caagaaaagc
     1501 aagagatgtt gcttcagttt tacaacacat tatggatggc gattacaaat taaatcaaag
     1561 taatcttatt tttgatggaa gaaaattatt ttttaatcta acaatttctt ttgaaaaaag
     1621 ctataatgaa atggaattca agaaaggtaa tgaattgatc gtaaactttg cagatttgtg
     1681 tagcggtgaa tgtttaatta atgataaatc tgtaatgtct tttggtgata gccaatatat
     1741 aagaaacttc aaaagtaaaa ttttagcaat gaaatcaaaa gaacaaagcc gaggagtctt
     1801 ttctaagggc ggtcatggtc gaaaaagaaa attagatcaa gatcgatggg aagcaataaa
     1861 gaaaagagaa agtaattttg ttaaaactta taataatcaa tgttctgcaa aaatcgttaa
     1921 aactgctatt cgcaataaag ctcaaattat tcgtatcatt gaaccagata aggcttatgg
     1981 cgaatgggct tactatcaat tgaaagaaca actagtaaac aaaggaaaaa gatatgggat
     2041 agaagtcaga agttagtaaa ttaaacaatc gttgatttgg caacgaaata gtgagggaaa
     2101 atccactcac tgaagggtaa actccgtcaa gttgtgtgac cagacggaag gacgaatgtt
     2161 gttgaggttt tagaaccata taaaacagca tgtttctcaa atttttagga taagggaaat
     2221 tgctagcagt ttgttttaga accatataaa acagcatgtt tctcaaatga gtactttaaa
     2281 ttaattgatg gcgttcctgc tttagaacca tataaaacaa catggattta aaacaagaag
     2341 taagtcagtt ctccttttac tttagtaagg aaaaataaag tattctcaat aaaacttaaa
     2401 ataagcaaac ctttgtttta aaatgattgt caagttaaaa aaatctggga tcatataaaa
     2461 tagtatagtt ctcaaatatg attagaaacc ctattaaaga gcaatattaa gccaacttac
     2521 ttaaatcgtt taattaagaa caataatctc ttttagtcag acttttagtc tgactttttt
     2581 gtatttccaa aatttattct ttacaaaaac gaacatacgt ttgtataatt acttgtgaat
     2641 gggggaataa aaatgtttga ttacagtaga gaaccggtga atgactattt tttaatcgac
     2701 atgaagtctt tctatgctag cgtggaatgt attgaacgaa atttagaccc aatgactaca
     2761 ccgttaattg tcatgagtag atcggacaac actggttcag gtctgactct agcttcttca
     2821 cctactgcaa agaaaaaata tcatattact aatgttagta gagctactga tttaccttat
     2881 ccattaccaa aagaacttca tattgttcca ccacgaatga acttatatat aaaaaaaaat
     2941 atggaagtta atcagatttt tagaaagtat gtcgcagatg aagatttatt gatttattcg
     3001 attgatgaat caattttaaa agtgacaaaa tccttaaatt tatttactat ataaattgag
     3061 ttaaattgta ttcgtttttc ctcctatagg cgtatactaa agaaaacact tgaggagtgg
     3121 ggaacatgga aacagaacgc caaatccgtg aattggaaaa agcgatgaat acgacagaca
     3181 accgccggtt gtacgagcgt ttcctggcag tcaagttggt tcttgaaggc cacacccgga
     3241 aagaagccgg gcacaccatc ggccgcgacg agcatactgt cagccactat gtgaaaaact
     3301 actgccataa tggaatccaa ggtttagtct ccaaaaaaca aagcggacga ccttcacggc
     3361 tgacagatga acaggaagcg gaactggtcc aaatcgtcgc ctaccacact ccggaggaag
     3421 taggtttcaa atcccgtgcc aattggacat tggcgattgt gtgtgacctg attctccggg
     3481 aatggagtta tggatatacg caaaaaggcg tggcggacct ccttcaccgg ctaggactaa
     3541 gctggacccg tccgacgtat accctcaaaa aagcggattc caagaaacaa caggtatttt
     3601 tggaagaaac cttcccgagc ctaaaaaaaa attgctgaat agcgatattg accacctcct
     3661 attcgaggac gaaaccatga tccgggatta ccaagccatt ggctgcacct ggtttctccg
     3721 cggccaccag cgaaaaatcc ccacttatgg acaacacaaa ggggtaaaat tgataggcac
     3781 cttggattat gaaaccggtc ggatcatttg tacagaagaa ggggcatacg acgcagcggc
     3841 cttcctccgt tttctggagc ggacgttgcg ggaatacccc accggaaaaa tcgtgatggt
     3901 attggataat gcccttatcc accacgccaa gctgatccag tcttttcttg aagaaaacgc
     3961 taggcgcctg gcactcatct tcctccctcc ctacagccct cagttgaacc taatggaagg
     4021 cgtttggaag tggctcaagg aatcggtcat caacaatgtt ttttttgacc acgtccagaa
     4081 aatcaaacag tcggtccgcg gtttcttagc cgatgtaaac gagcgtcccc ttgaggtgat
     4141 tgaccgactg tgcgtgcgga tgtaaagtat ttaattcaac ctatatagtt caggaacaag
     4201 aagcgagaga agaagagaat tagctaaaat aatcaaagat gagattcaga aagaaacagg
     4261 tctaatagca acagtcggaa taggagataa cccattatta gctaaacttg cactggacaa
     4321 tgaagcgaag agtagtaaag aatttatagc tgagtggact tatgagactg tacctgaaaa
     4381 aatatggaaa ataaacgaga tgactgactt ttggggcatt ggatcacgca tgaaaaaaag
     4441 attgaatcat atgggaatta tgagtataga agatttagca aattggaatc cctacacaat
     4501 taaaagtcag ttaggtgtaa tcggactcca gttatatttc cataccaatg gaattgatcg
     4561 tacggatatt gctattccac cagaaccaac caaagaaaag tcttatggga attcacaagt
     4621 tctaccaaaa gattatacga ggagagaaga agtagaacta gtagtcaagg agatggcaga
     4681 gcaagtagcc attcgaatta gacaacacaa ttgcaaaact ggttgtgtac gcttagcaat
     4741 tggtacgtca attttggaag aaaaatcagg tttctcgcac cagatgaaaa taccaattac
     4801 agataatacg aaagaattac aaaattattg tcttttttta ttcaataagt tttatgaggg
     4861 gcaggaagta cgacatatag gagtaactta tagcagatta gtatacacag attccctaca
     4921 attagattta tttagtgatc caagaaaaaa gattgctgaa gaaaatttgg ataggattat
     4981 tgataagatt agacaaaaat atggatttac agctattata catgcttcat ctatgttaga
     5041 aggtgcaaga agtattacga gaagcacttt aataggagga cacgcaggag gattaggagg
     5101 tttgcataat gattgaatat aacgatagag gacgtttaaa atggtcggga ttttatttgt
     5161 ctgatcatac tgaaagaatc aatgcagaca atgtacaacg tgctaatcat aatttggcaa
     5221 aagaacaaat gacgactgaa gagattgcga aaatactgag aactgctgtc ataaaaaata
     5281 gacccgtctg cattcaaaaa gaggaacgaa acgctgaagg ttactatcca gatgatgtag
     5341 taggaaaaat tattggatat gacgagcttg gtgtatttgt ggaagaagaa aaagttcact
     5401 atgatgaaat taggaatgtt tctttctcgg aattattaaa atggagtcag atatgaacat
     5461 tattaaaaaa tacgaattcg gttatattac gtatgaacag ctaatagagg aattatgggg
     5521 atatggtcaa caattaatta atgaagtggg gattgattgc ttttgtttct atattgaatc
     5581 cggagcagga taccataaga acagatacta tatatttcca tatccaccaa aatagtctct
     5641 tagatatttt taaattttct ccaaatactt tcaattattg tgaaatattt ccctttaatt
     5701 aatcaaattt tttataaaag taatatataa aataaattgc ttgaagtatt atatttagat
     5761 atcataattt atggaagaaa aattaattaa aaaggatgag attataatgg ggttactggt
     5821 tccgagagct aatacttata ctataccttt aacgaaacgc caattaattg ctaaaagata
     5881 tcaaaggatt actagagcca tcaatagaga gttttggaat tctgaaagtg atactgctca
     5941 cagtttatac gtaggttctt atgggagagg aacggcaatt agtactagtg atatagatat
     6001 aatagttgaa ttaccgatgg cagaatttga cagattcaaa aattatttat caaatggccc
     6061 gtctaagtta ttacaagtta taaaaaacgc atttcaggaa atactaccaa atagtgatat
     6121 tagagctgat ggacaagttg ttaaaataaa tttccatgat ggaatcaaat ttgaaatagt
     6181 tccagctttt aatgaaaaag attattgggg tgagtcaaaa gggtttattt atccagattc
     6241 aaacatggga gggaactgga aagctacaaa tcccaaaaaa gagcaagaag ccatgaaatt
     6301 aaagaataca aaaagtaata atttattata tgctacgtgt aaacattttc gacatgtacg
     6361 agatacagaa tttacgagtt atcatctttc aggaatagta attgacagtt tcgtatatga
     6421 ggctatggga aattggaaat tcgttgaaaa taacagtggg ggtcaaaaca ttagttctgt
     6481 atcttatgag acagctttat tagaatacta taactcacat aaagttatgg gcggtttaaa
     6541 tttatactct cctggaagca atcaatttgt taattctgat agtagtatca tatgtttaga
     6601 aaaagtatta aaaaaaatag ctttataaag ttttatggag gaacaacatg ggagacgaaa
     6661 aaaagcgctt ggaagttcaa ttacgtgaag catatggacg agtaacgtat acttacacca
     6721 ctcatttaaa gcaagccgat aatattattt ctgacaataa aattttaagg tgtcttcaaa
     6781 ttggtttgtc agcccttact actggagggg tacttagtac cattattact gataatagtg
     6841 agttaacctt tgttgctgga ttatgttcaa caatttctct tggattaaac ttatacttta
     6901 agaattttga tcttgctgaa gaagctaaaa atcatcaaat tgttgccgat aaattatggt
     6961 tgatcagaga agaatacgta agcttactaa cagatttaaa tcgtttaagt gttgaagata
     7021 ttgtgaaaaa aagagatcaa ttaatgaacg aaactcatga agcatataaa aatgccccta
     7081 aaacaaatag taaaagctat gaatcagctc agaaagcatt aaaatctgaa gaagagcaat
     7141 tttttacaga agaggaattg gataagatgt taccagagca tcttagaaaa aagagttcta
     7201 aatattgaac tcttttttta tgcttaaaat tctcttgtaa tttttctatt catctatatt
     7261 taagaagaaa tatgatgata gaaacatctc ctaaagccac cgcaagccgt tctaagcata
     7321 gcttagcccg acattgctag atatgctcat ctcaccatga acgtttccta aagccaccgc
     7381 aagccgtcct agccattgcc tagtccgaca tcgctagatt agtgcatctc gccataaaag
     7441 ttttctaaag ccaccgcaag ccgttctaag catagcttag cccgacattg ctagatatgc
     7501 tcatctcacc atgaacgttt cctaaagcca ccgcaaaccg tcctagccat cgcctagtcc
     7561 gattttgctt gatcggttcc atttctgttt atttagtggg tcaacaccgt aatcttaaat
     7621 agccatcgcc tatttaagat tactaggtct gctttgaagg taaaaagtct ttcattcttc
     7681 aagactttta ggctttcaag ctacgcctga aagcattgaa attcatcgct ctttatttgg
     7741 ttttgccatt tataattcct tacgcccatt atctttttcg cctgtcatac cttgtcagaa
     7801 gtgtttagag cttatctcat ctttggatta atacaagtgg cgtaaatttt tttctaaacg
     7861 caagcgttac gaaaataatt tacttaaaca cttgtattaa tccggcagat aagaacgcat
     7921 tctaatcact cccgacaagg caagacaggc gaaaaagata aataaatgaa cgaaaggaag
     7981 aaacataaaa tgcaaaacca aatagcgatg aagaaaaaac cacctccctc taaaacaatc
     8041 caaacaaaaa agaattttga aaaaccaaaa atcaaaaaca gaaagaagcg ttcaacatga
     8101 gagatagaat tttagaagtt aatcaagaaa tggtaaacaa gttaacaaat atcagtggga
     8161 actatgaaaa gtacgatggt actttacttg ataactatat ttttcacgat acacaaaata
     8221 tcagaattgg cagaagtaaa ccgagagcgt tcattctcat caaagagaaa tatttgaatg
     8281 aatggaatag cacctatgaa atggttatga ctgataactt tgaaaaggta ttggatttca
     8341 gaaatgcttt gttgggttct gatatgggaa atagcagtct tgaatctatt gaagttgaaa
     8401 acaagaccga gaatgaaggt gaaggattga ttaagaatgt cttgaaaatc gaacaaaaat
     8461 atatcagtga agatagaagt ttttatagag tcacaagctc ggttggtcta gcaaactttg
     8521 ctactaactt aattggaaac aatgctagtg aatctttgct agtgatgtgt ctgaatacaa
     8581 agaatgaggt cattgcttat agcgaagttt tcaaaggtgg aattaatcaa tcagtagcga
     8641 taccaagaga gatttttcaa ctagctttgc taaacaattc agcaaaaatc gcagtatcac
     8701 ataatcatcc gagcggatat gaatttccaa gtgaaaatga caaaagtttt actacgaatt
     8761 tgaaaaacgc aggaaaaaca attggtattg aattgttaga ccatatcatc gtaacaagta
     8821 aaaacacaag ctattactca tttcaagaaa atggttcttt ataaaaatta gagaaaagag
     8881 gaaaacaaga tgggcgaaag aacacaagcg ttaattacag tcaaatatgg taacagcaaa
     8941 acgaaagcaa gcgtgtcgat tcattatcag tgggggtatg gtcgatatat gctgatggac
     9001 gtattgaatc aaggaattta tttaatcaat aaagcatacg acttacgaga taaatcaaga
     9061 gaagaattag caaaaggaat ctttgataga agtacaggag ttatcaattt tgttacctat
     9121 gaatctggag tgagtgctta tgatgaaaca gggtttggtg ggcgctatga accttatcaa
     9181 gaaacgattg aagatgtgat gaagttttca gataacaatg atggctatgt tcatttagag
     9241 atggtgttcg atgaaaatgg atttagtcta gatgataaaa ccagattgcg attgtttgat
     9301 tttgagggtc aacagatagg actaaacaag ttttgtgaaa gcggaaaaga gtttgctacg
     9361 aaagatttta ttaagggata cagattattg ctagaaaatt atggaataaa cctagaagaa
     9421 gagagcgtga aataagtgtt tgaaaaaaga aaagaccagt tccctaattt agaagcgaaa
     9481 agtcaaaaag tgattgatgg tgaaatgatt gaattattcc aaaaaagtta tggaaccata
     9541 gaaactaccg caagttgcaa atactacttt caaggaaaga gaaacacgtt taaaggcttt
     9601 gccgattctg atgaagaatt ggcaaagaaa atcattgatt ggcttgtcag cagtgaacac
     9661 gtagcaaggg aagataccgt tagaacaacg attaatgggg aagtcctaac aggtactaga
     9721 tgtgtttgtt gtaatcatga atttgtagaa gatgaaggtt gctaccgaat caatgaatat
     9781 ggtgatttta tttgccagag ttgccacgag atagaatttc caaccgaaag aagttggttt
     9841 gaatacatgc tacaagatta tgagatggaa aaagaaaatg gtgaagtagt tttttgtacc
     9901 aaagaggaat tgagtaagag aagcgatgat gaaattgaag attggtgtgt gtggttagta
     9961 ggggcagatg attgcgttgc atattacaca acagcagaat aggagtgtga atcagcatgg
    10021 aaggaatgga ttttctagat catgaagatt tggttgattt tggttataca tggaaaggaa
    10081 tggtaggtat tagcagaagt cttgctaatg gcttttacga aagaaattat gcagtatatg
    10141 ttctttatga tgacgataca gaaagccttg ttgacgagga atataaattg gatttagaga
    10201 atgttttata tggaatagaa aaggaagatt tagcaaaata tattttttca tggttgggtc
    10261 aataagtgaa aggaagatag aaacatgaca aaatttaaaa gtaagggtat gcaatggtgt
    10321 tcttattatt atgttggaaa tgatgaagat ttattgagaa aaacccaacg aaaaggttat
    10381 ccagataaga tattgaaaca aataggttat tacatgccag aagagaggat agaaaaagaa
    10441 ttttgtgagg ttctttttga tagtgaacag ctattagttt tgttaaccca aaataaattg
    10501 atttgccaat ttgaaaataa ttaagatgag gtggtgaacc atgaatgaag ataagatggt
    10561 attaagtgaa tatgctagat tgagaactat taattcttta gcatctcatt taagttgttc
    10621 tttcctaaca aaggtgctaa aattaattta caaatgtgaa gattttgtac aagagaagca
    10681 caaagacaag cctactgcga ataggcttgt ctttttttca ttcattacga ttccgtatgt
    10741 gtgggttagc gacaatttgt tcaattaccg tatgatatag catcttaatt tgattagatt
    10801 acttgcaatg tagtttatta agagtataat ataatcataa atgtggacaa tttgataaat
    10861 aatgagtgtt ttatactcaa aaggagtgga tgaaatgaag gtcaaaaatc caactgaaaa
    10921 aagcagaatc caagtcggaa tagacaagaa tttaaaagag aacgcagaaa tgatattgga
    10981 agagctaggt ttaaatccta caacagcaat aacaattctt tacaaacaag tagtcgctag
    11041 aggtgagttt ccagtagaga taaagctaag tgaagaggag aagcaaggaa tcagattgca
    11101 acagctaact aaggacatgc cagtagatgt tctagataca gatgaaaagt tagaagagtg
    11161 gttcgatgaa gcctaatgag atagtaacgg tatatgttgc atttacagat aaaaatggtg
    11221 gtaaaagaag acctattttg gtggttagtg ataaagaaga tagagtagaa ttctttggaa
    11281 tcacaagtca atatgagaaa aaatcagacc aaataaagag ggtgtatttt cctattgcag
    11341 aatgggaaaa aacaggactt aaaaagcaat catggattga cgtagggtct ttaaaggcta
    11401 ttcccaaaag ccgagagaac atatcattca aaaaggttgg tatgttatca gctagtgata
    11461 cgaaaaagct aaataggttt atcgagaaat tacaaagaaa agtctaacaa ggcttttctt
    11521 ttttgtttat atgtatatta agatatacgt ttatacaaac gtatttatgt atatgaaaat
    11581 atacgtaaat atatacgtga ttataaacat taagaaaacg ttaagaattg ttaaataatg
    11641 atatttagta atatacgaat tataatatat attttatata aaatgtatgt aaataaataa
    11701 aatgtctgac ggatattgaa tagcaataaa taacaataat tcagacgaat gcggtgagtg
    11761 aagtagcttg ctacgaacga acaggcagaa tgaaacggca gtttcattag agcaagtcgc
    11821 ttgcgacgta gcgttaccgc tgatgtaact ggcaagttac atagagagag tttgcttgca
    11881 aacgaacgtt acctatttga atagaaataa aaaataacag attagtgaat tttgaatagg
    11941 agccacaact caaccaaccg cttgcggtgg gtgagtccca cggaggaaaa gacgaacgaa
    12001 cggtcgcgta gc
//
LOCUS       DAXWLG010000072        11935 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXWLG010000072 DAXWLG010000000
VERSION     DAXWLG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.072"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(160..525)
                     /locus_tag="ACGE4T_001587"
     CDS             complement(160..525)
                     /locus_tag="ACGE4T_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386865.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            733..1215
                     /locus_tag="ACGE4T_001588"
     CDS             733..1215
                     /locus_tag="ACGE4T_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386866.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1412..2302
                     /locus_tag="ACGE4T_001589"
     CDS             1412..2302
                     /locus_tag="ACGE4T_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFF8386867.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2628..5102
                     /locus_tag="ACGE4T_001590"
     CDS             2628..5102
                     /locus_tag="ACGE4T_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8386868.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5205..6941)
                     /locus_tag="ACGE4T_001591"
     CDS             complement(5205..6941)
                     /locus_tag="ACGE4T_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8386869.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6938..7642)
                     /locus_tag="ACGE4T_001592"
     CDS             complement(6938..7642)
                     /locus_tag="ACGE4T_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8386870.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7773..8276
                     /locus_tag="ACGE4T_001593"
     CDS             7773..8276
                     /locus_tag="ACGE4T_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFF8386871.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8236..8574
                     /locus_tag="ACGE4T_001594"
     CDS             8236..8574
                     /locus_tag="ACGE4T_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFF8386872.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8595..10013
                     /gene="ffh"
                     /locus_tag="ACGE4T_001595"
     CDS             8595..10013
                     /gene="ffh"
                     /locus_tag="ACGE4T_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFF8386873.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10125..10703
                     /locus_tag="ACGE4T_001596"
     CDS             10125..10703
                     /locus_tag="ACGE4T_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFF8386874.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10707..11228
                     /locus_tag="ACGE4T_001597"
     CDS             10707..11228
                     /locus_tag="ACGE4T_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFF8386875.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11307..11861)
                     /locus_tag="ACGE4T_001598"
     CDS             complement(11307..11861)
                     /locus_tag="ACGE4T_001598"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8386876.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3921 a         2150 c         2464 g         3400 t
ORIGIN      
        1 atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa gaaaagaagt
       61 aaaaaataga aaaactagtg atagttttgg tgatataaga aaaacatata tgtataaaaa
      121 gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc gagctttttt
      181 ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt aaatacctgc
      241 aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc attcagtaaa
      301 attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga ataaagatat
      361 aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata tcaatgtgat
      421 gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca cataaggaac
      481 aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc atcaccacct
      541 aacaagtgtt attattttgt tagttttatt gtaaattgaa tcctgcaata atcaattact
      601 ttttcattat ttctagatta aatgaataaa ctctcatgcc attatctcta aaaaaaaagt
      661 ttttccccct aattttttta gatagaaaat tgtatagtat gatgagagaa aaagtagttt
      721 gggggaacgt taatgagaag aaaagcagta atcgatcggc tttatttatt tttttggttt
      781 tttcctatcg ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg tgcggagata
      841 ttaggcctga tccaaggaag cggattagtg gtttctagga attttccagc tttgactgtt
      901 ttctatttag tgataagtgt aggtctctta ttaccaaaat ctctgatcaa tcactacaat
      961 tggcagctgc tttacttttc ttgcttatta gtatttacat ctatgttttc tttgctgcct
     1021 cgcttgatta tcggccctaa tggtttgagc gagagtggat tgaaggattc agcttattcc
     1081 tccatgtatt tcttgacgat caaaccatgg ttctatttag taattgcctc atttattctt
     1141 gcaacatttt tttacttctg gacagaaatc aggataaaaa gtgaacgatc tgtatctata
     1201 aaaaataact attgatggtg gatcatcagt aaaaaagcat atgagctgcg taaaatgcat
     1261 gtttctcata tgcttttttt attattatgt aaagaatgcg aaaaacgttt acatgatttt
     1321 tacataactt aactatattt ttacagcaac catacgaggg atttacagtc gactgataaa
     1381 ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa gtattgttta ctctaggtat
     1441 gatgggaatt cttttagccg gatgcggctc aggatcagga aatacagatg gctctgccac
     1501 acaaaataat tcaagtgact caaatgaaca agtaaaaatc gttgcggtag gttcgacagc
     1561 attacagccg ttagtcgatg cagcacaaga aagctttgta caagaaaatc caaattatca
     1621 aatctctgta caaggaggag gaagcggaac tggtttgagc caagtcgaag ctggagcagt
     1681 tactatcgga aactctgatg tatttgcaga agaaagagac ggagttgatg cttcaaaatt
     1741 agtggatcat aaagtagcag tggtcgggat ggcaccaatc gtcaataaag atacagatgt
     1801 caaagatatc accaaacagg aattgattga tatctttaca ggaaaaatca ctaactggaa
     1861 ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg gcagcggtac
     1921 ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt cccaagaaca
     1981 agattcatca ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa tcagctattt
     2041 agctttctca tatctcgatg attccacaca agcactaagt atcgacggtg tagaaccaaa
     2101 agaagagaat gtagcagata acagctgggg aatctggtca tatgaacata tgtacacgaa
     2161 cggaaaacct tcccctgaag tccaaaaatt cttagactac atgatgacag aagaaatcca
     2221 agaaggacct gtcaaagaat tgggctattt accaatcaca atgatggaag tagaacgtga
     2281 tcacgaagga aatatcaaat aactagcaga taaacatgcc acggtagtcg ttactattaa
     2341 ggaaaaatca actatgtttc cccacatata catttttcct ttaattgtga aaaatagttc
     2401 ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttatt
     2461 cgaaaaaaag agctacgaca tagaactgtc gtagcttttt ttttgctata tgacaggaat
     2521 gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta tactggatag
     2581 gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg aagaataaat
     2641 ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa gtcatccgtc
     2701 tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga atagaaaaag
     2761 gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc gtattggaaa
     2821 acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt tgtttagtag
     2881 aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga atcgccattt
     2941 ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat acagcaaaag
     3001 tcaatgtcat acgagacggg atagaacaaa caatagatat agaagacctt gtaatagatg
     3061 acattgttat tttagagact ggacatcaaa ttccagcaga tgcagaggtc gtggaaggga
     3121 aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc aaagaaatag
     3181 gcgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc cgattagaca
     3241 aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca atgggagaag
     3301 gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt gggatcatca
     3361 ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga aacacgatca
     3421 aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa ggactgtact
     3481 tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa agagttcttt
     3541 tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt gtagataaaa
     3601 caggaacgat cacagaaaat aaaatgtctg ttcaaaaagt gatcgtctct aaaaaacaag
     3661 aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca aaagcgatgg
     3721 caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc actgatcaat
     3781 cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta gcgttggcag
     3841 acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg ccggaatatg
     3901 cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt ggtgtgtacg
     3961 atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc tatattctga
     4021 ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa aaacagcaag
     4081 tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta gctgtccaag
     4141 cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa gaagaatatg
     4201 aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa aagaaaatct
     4261 tcgttcaatt gctaaagcgt cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     4321 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     4381 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     4441 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     4501 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     4561 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     4621 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     4681 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     4741 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     4801 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     4861 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     4921 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     4981 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     5041 agcaaaagcc gtttagtcgc gtagttaaaa aatttctatc gatatttaaa cgagaaaatt
     5101 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     5161 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     5221 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accatgaagg
     5281 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgccggctt
     5341 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     5401 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     5461 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     5521 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     5581 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     5641 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     5701 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     5761 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     5821 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     5881 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     5941 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     6001 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     6061 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     6121 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     6181 tcgttcagta acgtatataa ttgctcttct gtggtagtat acgcccgata catcttactc
     6241 atttgttcgc tcaacgcatt gatcgtctga tacaattctt cccattgatc gggtgtttgc
     6301 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     6361 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     6421 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaccg
     6481 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     6541 gcgacatata aaagatcccc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     6601 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     6661 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     6721 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     6781 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     6841 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     6901 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     6961 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     7021 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     7081 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     7141 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     7201 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     7261 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     7321 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     7381 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     7441 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     7501 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     7561 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
     7621 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
     7681 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
     7741 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
     7801 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
     7861 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
     7921 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
     7981 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
     8041 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
     8101 gaacccattc gatttatgac gggagttttt ccggaataaa aaaaaatggt gccggagtgg
     8161 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
     8221 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
     8281 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
     8341 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
     8401 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aacttcatct tttttctaat
     8461 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
     8521 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
     8581 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
     8641 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
     8701 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
     8761 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
     8821 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
     8881 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
     8941 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
     9001 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
     9061 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
     9121 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
     9181 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
     9241 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
     9301 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
     9361 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
     9421 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
     9481 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactat gacgagaaaa
     9541 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
     9601 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
     9661 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
     9721 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
     9781 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
     9841 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
     9901 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
     9961 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    10021 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    10081 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    10141 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    10201 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    10261 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatattaaaa
    10321 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    10381 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    10441 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    10501 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    10561 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    10621 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    10681 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    10741 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    10801 gtaagcaaac cacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    10861 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    10921 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    10981 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    11041 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    11101 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    11161 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    11221 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    11281 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    11341 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    11401 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    11461 aagacaagtg attgtcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    11521 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    11581 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    11641 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    11701 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    11761 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    11821 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    11881 actatctata acaatcttac aaaaaataag taactggagc aaataaaaac actta
//
LOCUS       DAXWLG010000073        11761 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXWLG010000073 DAXWLG010000000
VERSION     DAXWLG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.074"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            118..393
                     /gene="rpsP"
                     /locus_tag="ACGE4T_001599"
     CDS             118..393
                     /gene="rpsP"
                     /locus_tag="ACGE4T_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFF8386877.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            405..656
                     /locus_tag="ACGE4T_001600"
     CDS             405..656
                     /locus_tag="ACGE4T_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFF8386878.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            780..1571
                     /locus_tag="ACGE4T_001601"
     CDS             780..1571
                     /locus_tag="ACGE4T_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386879.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1741..2262
                     /gene="rimM"
                     /locus_tag="ACGE4T_001602"
     CDS             1741..2262
                     /gene="rimM"
                     /locus_tag="ACGE4T_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFF8386880.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2252..3013
                     /gene="trmD"
                     /locus_tag="ACGE4T_001603"
     CDS             2252..3013
                     /gene="trmD"
                     /locus_tag="ACGE4T_001603"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFF8386881.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3149..3496
                     /gene="rplS"
                     /locus_tag="ACGE4T_001604"
     CDS             3149..3496
                     /gene="rplS"
                     /locus_tag="ACGE4T_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFF8386882.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3806..4048
                     /locus_tag="ACGE4T_001605"
     CDS             3806..4048
                     /locus_tag="ACGE4T_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFF8386883.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(4136..4282)
                     /locus_tag="ACGE4T_001606"
                     /pseudo
     CDS             complement(4136..4282)
                     /locus_tag="ACGE4T_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            4339..4653
                     /locus_tag="ACGE4T_001607"
                     /pseudo
     CDS             4339..4653
                     /locus_tag="ACGE4T_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            4682..5941
                     /locus_tag="ACGE4T_001608"
     CDS             4682..5941
                     /locus_tag="ACGE4T_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8386884.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            5994..6890
                     /locus_tag="ACGE4T_001609"
     CDS             5994..6890
                     /locus_tag="ACGE4T_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386885.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPESAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            6901..7770
                     /locus_tag="ACGE4T_001610"
     CDS             6901..7770
                     /locus_tag="ACGE4T_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8386886.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            7780..8455
                     /locus_tag="ACGE4T_001611"
                     /pseudo
     CDS             7780..8455
                     /locus_tag="ACGE4T_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            8661..10325
                     /gene="alsS"
                     /locus_tag="ACGE4T_001612"
     CDS             8661..10325
                     /gene="alsS"
                     /locus_tag="ACGE4T_001612"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFF8386887.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            10338..11048
                     /gene="budA"
                     /locus_tag="ACGE4T_001613"
     CDS             10338..11048
                     /gene="budA"
                     /locus_tag="ACGE4T_001613"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFF8386888.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            11178..11675
                     /locus_tag="ACGE4T_001614"
     CDS             11178..11675
                     /locus_tag="ACGE4T_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFF8386889.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         3910 a         1925 c         2473 g         3453 t
ORIGIN      
        1 tctatttcct aaaaaaaatc gaatttaatc aagctgtaaa gaaaaaaagc tttacagggt
       61 aaaaaaaaac tgttacacta catcttgtga ataaaacaat ggaggtggaa ataaataatg
      121 gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga gtccgtttta ccgtatcgtc
      181 gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg aaactgtagg tacttacaat
      241 cctttgaaag accctgcaga agtagtttta aaagaagact tagttcttga ctggttatca
      301 aaaggtgcac aaccttctga cactgtacgt aacatccttt caaaagaagg cgttatgcaa
      361 aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc ggatatgaaa gatttaagcg
      421 acttagtctt aacaatcgtt cgtccgctag tcacttatcc tgatcaagtc aagttagacg
      481 tcgtagagtc gaaagacttt tacgagtaca atctaacagt ggctcctgaa gatattggcc
      541 gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg cacaatcgtt tacggtgttc
      601 ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga tgataaggaa aagtaaataa
      661 acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt ttttattaat tgaagaaatg
      721 attatttggc aaaacaagca agaaagcgta tccttatagg aggagggagt gaacagccaa
      781 tgagaaagaa aatgttattt attccatttg ctctattgtt aagcgcttgt agttcggtta
      841 ctcaagaatc agacaataca catctatcaa gcagtatatc tgaaactgtt accagtccag
      901 aaaaaagtag taccaaaacg gctaccacca ctgaacaaac aatggctacc tcaaataatg
      961 agaaaaagac tgctttagac cagttgaaag aacaacagcc aaatgttccc atgcctctag
     1021 atgtaccagt ctcttcaggc tatctcaata tcgcagcgac acatacaaag caaggttatt
     1081 ctattttata ttacagaaca gatcgtcccc tcgggttgaa tgctgacgaa ttgaatcaag
     1141 aaacgcctat tgcaacctat ctatatcaat atgggtttgc ttctagtcaa gaaacaatcc
     1201 aagttttgca gccctttgag atcgacacaa atggtcagca agtggactta ggttctcgaa
     1261 tcacagggta tcagcaaggt gcagcaggtt caagttttct tgaatggcaa gaaggaaact
     1321 ggcgtattcg aatccgtggg aataatatag aaggccaaga tcccttacta ttagctaaag
     1381 aaatcgtagc ttatttggaa gaaaacagtc taccagcacc tgaacaattt ggtaaaatta
     1441 ctgttgatat gggtgatacg acgaatcgag ccgtagaagt aagctggcaa gaaccgaaaa
     1501 atgcctatac gatcactcat caagatccta tgtctgcgtt gaagatggca gtttctatga
     1561 agaggttgtg aaatcagcgc gctgatctga gcccatcatt taactccaag tcacaggaag
     1621 taatcaaaaa tagcacgccg catttttgca taattttggc ttaatacctt aaaagaaaac
     1681 tagaattgcc tcttcacaca tgctaaaata aaacagacaa aaaagaaatg gggatataag
     1741 ttgactgaat acttgaatgt aggaaaaatc gtaaacacac aaggtatcaa aggcgaagtc
     1801 cgtgttatct caacaacgga tttcccagaa gaacgttaca aaaaaggaac ggtgttaaca
     1861 ctattccaag acggaaaagc acctgtggaa ttaacagtca aaagtcatcg caaacataaa
     1921 aattttgact tattgagttt tgaaggacat ccttctatca atgatgtgga aaaatatcgg
     1981 gatggcatat taaaagtgtc aaaagacaac tctgtagatc tagctgaaaa tgaattctat
     2041 taccatgaga tcatcggtct gcatgtatta gaagatggaa ctgagttagg taaagtaaaa
     2101 gaaatcttgt caccaggtgc caatgatgtt tgggtcgttc aacgtccgaa aaaagctgat
     2161 atattgcttc catatatcgc ttctgtcgtc aaagaagtcg atttagaagc tggagtggtc
     2221 cgtgtagaaa ttccagaagg actgattgac gatgaagatt gatgtattga ctttatttcc
     2281 aagaatgttt gaaggtccta tgggggaatc catcatcggt aaggctgtag ataaaggatt
     2341 attagatatc aatatctcta acttccgcga ttattcagat aacaaacatc agacagtaga
     2401 tgattatcca tacggcggtg gagcaggtat gctgctcaaa gtccagccca tctatgataa
     2461 catccaagcc attgaaaaag ctgcaccgga tgtcaaaaaa agagtaattc ttcttgaccc
     2521 agctggtaaa cgattcgatc agaaaatggc agaagaattt tcacaagaag aacacttagt
     2581 attcatttgt ggtcattatg agggatatga tgaacgtatc cgcacattag tgacagatga
     2641 agtttcctta ggagattatg tccttactgg gggagaatta ggtgcgatgg tcatgatcga
     2701 tgcaacagtt cggcttttgc cagatgtgtt agggaatcag acttcagctc aaacggattc
     2761 ttattcaacc ggtttattgg aacatcccca atatactcgc ccggcagaat ataaagggat
     2821 gaaagtacca gaagtattga caaatggaaa tcataaattg attgaagaat ggcaattaaa
     2881 agaatcacta agaaggacct atcaacgtcg gcctgatctt ttggaaacac ttgaatggac
     2941 acctcaaatg acgaaatttt tagaagaaat caagaaagaa gaacaagaaa aaacagcaga
     3001 tgaagccaag tgactttttc taaaatattc tttacagtag gttgataata tgttagtatt
     3061 ttatggtgag tgcaaaagca cttaactatt acgatattcc gctatgagaa aagctcataa
     3121 gaatatttgg aataaggaga agaaaaccat gaatccatta atcgaagaat taacaaaaga
     3181 acaattacgt tctgacatcc cagcgttccg ccctggtgac actgtgcgtg ttcacgcaaa
     3241 agttgttgaa ggtactcgtg aacgtatcca gttatttgaa ggtgttgtta tcaaacgccg
     3301 cggtgctgga atcagcgaaa cttatacagt acgtaaagtt tctaacggag tgggcgttga
     3361 acgtacattc ccattgcata caccacgtgt tgcaaaaatc gaagtagttc gctacggtaa
     3421 agtacgtcgt gcaaaattgt actacctacg tgcattacac ggaaaagctg ctcgtatcaa
     3481 agaaatccgt cgttaatcat tgttatcaga ctgagaaaga acccttggta ttcaagggtt
     3541 cttttttctt agcatgtaat ttgaacaccc tgatccagtt tttttcttta attattggta
     3601 ggggagaata atggattaga ataaatctac ctataagtag tagtaaaaaa aagagcacta
     3661 tgataatatc tttcttaaga gtaaccgctt taataaaaaa tccagaatat tctaacataa
     3721 atggagtatt cttagaatca aaaggagaaa gaagatggtt tatacttata cggagcaaga
     3781 aaaaatgaaa gtttgagaca cattcatgaa tgatggaaaa gtaaaacaag taccaagttc
     3841 aacaaaaaag aaaaacatcc tattgaaaga agtcttgaaa cgctttgacc atggagtgac
     3901 ttatacagaa acagaggtta attcgatact gctgaacgta ttttctagtg gcgattatgt
     3961 cgaacaaaga cgttatctaa tcacatttgg ctttttcaaa agaagttcag acggtcgggc
     4021 atatcagatg atgggaattg aaaattagaa gttttaattt atactcattg aaaaaagttc
     4081 aagtatgcga tattggttct acaccgcata cttgaacttt ttttgtcatc tgtatctata
     4141 atacaaagcc aagaacgtta ttcaaaatat ggatagctat gataaaagct aatgagcgat
     4201 tcgcagttaa ataagcaatc gtcagtaaaa tcgttccagg agcgtacatg aaaaaatcaa
     4261 ttggatgcgt tacatgaata aagataattc tcttttattt gtacgaataa ataccaaaac
     4321 gtacctccta aaattagatt caaagaagat agcaaagagg aaaaagatag ttctgcagca
     4381 gctatagcgg catgtggatt gctagaactt tccaaacaac tgcctgtttc agatcctcgt
     4441 catttcgatt acgaagagat agcaatcaaa atcttggaag aattacagaa aaactataca
     4501 acgaaacgag ttaacggatc aaacggtctg cttctccatg ccgtttatga taaaaacagc
     4561 ttaaaaggtg tagatgaatg tgtgatctgg ggcgattact tttatgtaga aggaatcact
     4621 cgtctagcta aaacatggta ctgttactgg tgatgtataa gagaagagga ggattcatat
     4681 aatgaagcga tggaaaaaaa tgatgattgg atcaatagtt ttaggaatgt cactcgtatt
     4741 tggtgcttgt ggtaatcaag aagacagtag tgaagaatca gcaaatgaaa ttaccgtttc
     4801 gacatggaat tatgaaacaa cacctgaatt caaagcgctc tttgaagcat tcgaaaagaa
     4861 aacgggaatc aaagtgaaag cagtagatat tgcttcagat gattatgata cgaaactcac
     4921 cacgatgctt tcttctggag acacgacaga tgtattgacg atgaaaaatc ttttgtcgta
     4981 ttcaaattat gctttacgag atcagttagt cgatcaaaca gaacgaatta gagaattaga
     5041 cactgatgct gctgaagaaa catatgaaat gtatgatatc gatggaaaaa catatgcatt
     5101 gccatatcga acggactttt gggtactata ttacaataag aaaatgtttg atgatgcagg
     5161 aatcgaatat ccaaaaaatc taacttggga cgaatatgaa gatttagcaa aaaaacttag
     5221 taaaaatgac ggacaagttt acggagcata tcaacatatc tggcgttcga caatccaagc
     5281 tattgcagct gctcaaaacg aggcgaatct tgttgaacca gattatcaat atatgactga
     5341 ttattatgat cgagcattga ggatgcaaaa agaaggcgcg cagatggatt ttggcacggc
     5401 taaatcaacg aaagtaacct atcagtctca atttgaagaa caaaaagcag caatgatgta
     5461 catgggaact tggtatatgg ctggcatact cgcaaacaaa gaagcaagta agacagaagt
     5521 agaatgggga attacagcta ttcctcagaa gaaaaaagga gaatcggtca cgacgtttgg
     5581 ctcaccaaca gcatttgctg tcaataaaaa ctcaaagaaa caaaaagcgg cgcaagaatt
     5641 tattgaattt gcagccagtg aagaaggagc aaaagtatta gcaggagtag gagtagttcc
     5701 ttcatatcgt acggatgaga ttgatcagct ttattttaat ctcgctggaa tgccaacgga
     5761 tgaaatttct aaaaaagctt tttctcctga tgaaatcaaa cttgaatttc cgattgatac
     5821 gtatgggcct gcaatcgata agattttaca agaagaatat gacttagttt tggttggaga
     5881 tgaaacgccg gaaaaaggaa cggtaaatat ggagaaacgt gtagctgcgg aaaaagaata
     5941 atcatgaaag gagagaagcc aagagataaa acactcttgg tttcgtcatc gaaatggaga
     6001 atagtataaa taagaaagct acaaatcatt tgcgtagaaa acgaacgtgg atcgcttatt
     6061 cgtttatcgc cccgaatttc attggttttt tcgtttttac attaatacca atcctgtttt
     6121 cgcttgtact agcatttatg aaatgggatt ctttttcaac gccagaatct gctggactag
     6181 ataactttcg taaaatggcc aacgatgaga cattttggat ttctttgaaa aatacttttt
     6241 tatataccgc tgctgtagtg cctggcacac ttgtttgttc gttagggcta gctttactac
     6301 taaatcagaa aattagaggg atgaagcttt ttcgtacgat atttttcttt ccgtatgtta
     6361 cttccttagt agcaattgct gttgtttgga atatgttgtt tcatccaaca gtgggaccta
     6421 ttaatatctt tttaagacga tttattgaac atcctccagg ttggacttca agttcaaatt
     6481 gggcgctttg ggcaattgta attgtcagta tttggcgagg gatgggttat tacatgattc
     6541 tataccttgc aggactacaa agtatccaaa aagaactata tgaagcgtct tcgctagatg
     6601 gagcaaaccg ttggaaacaa tttttgaatg tgacattacc ttcgttgaga ccaacaacct
     6661 tctttgtaac gatcatgctt gtcatcaatt gtttcaaggt gtttgacctt gtgcaagtga
     6721 tgacggatgg cggccctggt cgagcgacaa atgtgctggt ttaccaaatc tacaatgaag
     6781 cattcaaaaa atttgatttt ggttatgctt cagctatttc acttgttttg ttcgtaattg
     6841 ttttgggcgt tacggttgtt caattttctt ggaataagcg tcgtacttag gaggtgtgat
     6901 atggaagaga gtgttcgaaa tgaaagaaaa aaagcaaaaa ttcgttcaaa aaacatgccc
     6961 tttattcaat ggttgggtat gattctgctg attgtattag ctttattgac gatcattcct
     7021 tttttatgga tggtttctgc ttcattcaaa gcaaataatg aagtgttaac gattccgatt
     7081 caatggatcc ctaaaacttg gcatttagag aattatctgg ttatttggtc tcgtattcct
     7141 ttagtgacat tttttaagaa tacagctttt ttaagcgttg tgatcacgtt gattcagttg
     7201 tttacttcta gttttgctgt ttacgggttt tctgaactaa attttaaagg gcgcgatatt
     7261 ttatttttag catatatcgg cacgattgct gtcccttggc agtcttatat gatcccgcag
     7321 ttcattatga tgagaaaagt gggtttaaca gatacgttgt ggtcgttggt gctattacaa
     7381 gcgttcaatg cttttggtgt gttcttgttg aagcaatatt acagtagtat cccagattct
     7441 ttatgtgaga gtgcgcggat cgatggctta tcagagtggg aaatctatcg caagatcatt
     7501 ttacctctta caaaaccggc gattgccagt ttgacgatca tcacattcgt taatacatgg
     7561 aacgattata tggggccatt catttatttg tcttctacgg aaaataaaac aatccagtta
     7621 gggttgaaaa tgtttattgg gctatttgat gcagagtacg cattaatcat ggctgcctca
     7681 gttgtttctg ttcttcctgt tgcttttata tttttagcga tgcaaaaata ttttgtagaa
     7741 gggatcgctt catcaggaat gaaggaatag gaggtgatta tgaaagagca gcgttttgac
     7801 aaaaatgttt atatggaatt cttttattgg ttgtatcaat cattggcaag tagcctttgt
     7861 ttttggtgtg tcaatatctt gtatatagca gctgcgttcc ttttgtcgat agatggaagg
     7921 aatgtctttt cctttatttt ttctttactt tttattggtc caggttgcat cgtgatgata
     7981 ggtatgacag aagaattagc aaaagaaaaa agtttttcac cagtacgttt gttcataaag
     8041 acgtttcgcc aattttggct aaagggattt ttttattggc tatttgcttg gatagttagt
     8101 gtgataatga tttttgattg tttctttttt atacgttttt cttatggaaa atggttgatt
     8161 ccgttgtttg ttttacttgc ttgttttact tgcttgttta tcagtttcct ttagcatcaa
     8221 ctgctggtat tttcaggtga gaaatccagc gtcgaaacca aatcaagttc tacggatagc
     8281 gttctactat acgttgaaaa aatggtatgt gagtttacta gattttttac tattgacaag
     8341 tttgtttctc ttctttttcg taaagcccca atggtgtatt ctactaggtc cttctatcgt
     8401 atttggtttg atttacttca acaatcgtaa attgatgaga actatggatc tgtagacttt
     8461 ataaattgaa actttcaatt aagaatgaca gttttttatg taaaaactgt cattcttttt
     8521 cattttttga aacatatggc taaacaaatg aaataaaaca tgctatactt ttgaatgaaa
     8581 caggtttaga aactgtatca cttatttgtg attatttcta actaataatt aaaaataaag
     8641 cataaaaagg acgatgttca atggctgaaa aaatgaaaca aggttcgaaa tctgtagtag
     8701 aaagtttgat taatcatcat gttgattatg tattcggtat accaggtgca aaaatcgatg
     8761 gtgtatttaa tgaattagag gatcaaggtc ctgaattaat cgtgactcgt cacgaacaaa
     8821 atgctgcatt tatggcacaa gcaatcggac gtattacagg agagccagga gtagttatcg
     8881 caacaagtgg tcctggtgct agtaacttag ctacaggttt ggtaacagcg acagcagaag
     8941 gtgacccagt tttagcaatt gccggacaag taaaacgaag cgatctattg aagttgaccc
     9001 atcaaagcat ggataatgca gcactgttcc agccaatcac aaaatacagt gcagaaatcc
     9061 aagatccaga aacaatttca gaagtgattg ccaatgctta tcgtatggct aagagttcaa
     9121 aaaaaggcgc gagctttatc agtatcccgc aagatgtagt ggatgcacct gtccaaggta
     9181 atgtcatcaa gccattgagc gatcctaaat taggttctgc ttctgcagat gatatccgtt
     9241 acctagctga acgcattaga gaagcaaaac taccggtact attagtaggt atgcgaggat
     9301 caagtgaaaa agaaacgtta gccatcaggc aattagttga aaagacagca ttgcctgttg
     9361 tagagacatt ccaagcagct ggagtgatct cacgtgaatt agaagcgcat ttctttggtc
     9421 gtgtgggttt atttagaaac caaccaggag atatgctttt gaaacgaagt gatctagtta
     9481 ttgcaattgg ctatgatcca atcgaatatg aagcaagaaa ctggaatgcg gaaaaagatg
     9541 cgcggatcat tgtgatcgat gaagcgccag ctgaaatcga tccttttatg cagcctgaaa
     9601 gagaattgat cggtgatatc tcagccactc ttgatttatt gacaggttca ttagagcctc
     9661 aacaagtgtc agaagatgca aaagaatatt tagcatctct tcaagcaaaa ttaacggagc
     9721 gggacattgt tcaatcaaaa ggagaagcag gtatcttgca tcctttagaa gttattaaca
     9781 cgcttcagtc aaaagtaaca gatgatatga ctgttaccgt agatgtaggt agtcattata
     9841 tttggatggc acgccacttt agaagttatg aacctcgtca tttgttattc agtaatggta
     9901 tgcaaacgct tggtgttgca ttgccatggg cgatttctgc tgcattggtg cgtccaaata
     9961 cacagatcgt atcagtttcc ggcgatgggg gatttttatt ctctgcccag gatttagaaa
    10021 cggcggtacg taaaaaattg aatatcgttc atttgatttg gaatgatgga cactacaata
    10081 tggtagagtt ccaagaaaaa atgaaatatc agcgggcgtc aggtgtagac tttggtccag
    10141 ttgattttgt gaaatatgca gaagcttttg gcgctaaagg aatccgagct acaagtgtag
    10201 aggaattaga aaaagcttta gaagaaggat ttgcgacaga aggacccgta attattgata
    10261 ttccgattga ttaccgtgat aatgagaaat tgggagaaac aatcttacca gatcaattct
    10321 attaaggaag tgtcgacatg actgaaaaaa tcttatacca gcacgggaca ttaggagctt
    10381 tgatggccgg cttgatggat ggaacagaaa cgattgctca tatattagaa aaaggaactc
    10441 tgggattggg aacattgcat gggcttgatg gtgaagtcat ctttttagat ggcgtagctt
    10501 atcaaggaag atcagatggc tcagttgccc aattagatgg aagtgaactt accccctatg
    10561 cagcaattac agattttacg ccagatacta gtttttctgt ctcaaaaact gcagatggag
    10621 aacaattgaa aaaagacata ttagtaagag aagccgggga aaacctattt ttggcagtga
    10681 agataactgg attattcaaa aatatgcaca tccgtatcat gccgaagcaa gaaaaacctt
    10741 acagaagact agcaaaaatt tcagagtctc aaccagaatt ccaacaaagc aatgtccaag
    10801 gaacattagt cgggttcttt acgcctgaat tattccaagg agttgctgca gcgggatttc
    10861 acctgcattt tatcgatgat acacatacat ttggcggaca tgtaatggat tttgaagtgg
    10921 ctgaaggaaa agttgagatt agccgcatct cgtcccttgt acagcatttt ccagttgatg
    10981 atccgacttt tgttcacgaa acgattgatt atgctgattt agcagcagaa atccaacaag
    11041 cagaatagaa aaagacagcg agatgcgttc aaggtgatta acctgacgta tctcgttttt
    11101 ttgaggagaa aacctagcgt ttacaaaaaa actgagctat aatcttctcc atagatcaaa
    11161 aaggagcggt ttttatcatg tggacagaaa aacagaaaaa agatgcatac gaaattttat
    11221 tattacagca acaaggatta ttagaagcag aagataactg gttagcaaat ttagcaaatt
    11281 catctgcatt gttgaatgag actttgcctg agactgtctt tgcaggatat tatttattcg
    11341 atggtaatga attgatttta ggaccgttcc aagggcgagt ttcttgcaca cgtatcaaga
    11401 tgggaaaagg agtttgtgga gaatcagctg aaaaacaaga aacattgatc gttgatgatg
    11461 tcaaaaccca tgcaaattac atttcctgcg attcagccgc acaatcagaa atcgtagtgc
    11521 caatggtaaa agaaggtaat ttaatcggtg ttttagatat cgattgcgga gtaacgaaag
    11581 ggtatgatga aatcgatcaa gcatttctag aaaaatatgt agatctttta ttagcaaatt
    11641 ttaatataaa tgctttttca tttattaata actaaaatcg tctattgaaa caaaaaagaa
    11701 gagaaatcaa gaataaaaag agccggaaag acagtgggca tttctgtttt tcggtttttt
    11761 t
//
LOCUS       DAXWLG010000074        11680 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXWLG010000074 DAXWLG010000000
VERSION     DAXWLG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.075"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            224..724
                     /locus_tag="ACGE4T_001615"
     CDS             224..724
                     /locus_tag="ACGE4T_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386890.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(914..1258)
                     /locus_tag="ACGE4T_001616"
     CDS             complement(914..1258)
                     /locus_tag="ACGE4T_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFF8386891.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1412..2332)
                     /locus_tag="ACGE4T_001617"
     CDS             complement(1412..2332)
                     /locus_tag="ACGE4T_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8386892.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2519..3010
                     /locus_tag="ACGE4T_001618"
     CDS             2519..3010
                     /locus_tag="ACGE4T_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFF8386893.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3034..5409)
                     /locus_tag="ACGE4T_001619"
     CDS             complement(3034..5409)
                     /locus_tag="ACGE4T_001619"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFF8386894.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5735..6361
                     /locus_tag="ACGE4T_001620"
     CDS             5735..6361
                     /locus_tag="ACGE4T_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8386895.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6384..7088
                     /locus_tag="ACGE4T_001621"
     CDS             6384..7088
                     /locus_tag="ACGE4T_001621"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8386896.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7280..8113
                     /locus_tag="ACGE4T_001622"
     CDS             7280..8113
                     /locus_tag="ACGE4T_001622"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFF8386897.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            8310..10238
                     /locus_tag="ACGE4T_001623"
     CDS             8310..10238
                     /locus_tag="ACGE4T_001623"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8386898.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            10376..10831
                     /locus_tag="ACGE4T_001624"
     CDS             10376..10831
                     /locus_tag="ACGE4T_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFF8386899.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            10911..11039
                     /locus_tag="ACGE4T_001625"
     CDS             10911..11039
                     /locus_tag="ACGE4T_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386900.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            11301..11570
                     /locus_tag="ACGE4T_001626"
     CDS             11301..11570
                     /locus_tag="ACGE4T_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386901.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         3601 a         2167 c         2152 g         3760 t
ORIGIN      
        1 ctggaattct agacgctgaa gtaattcacg ttttctttct tctagagatc ataagtaacg
       61 aatgaacgaa cttcaaataa tttttttatt gtaattcata tttgtattgt tcgatgattc
      121 ttaatacgat atgacacaat aaataaaaat aggcatggct atttacaaca atagtgctat
      181 agtagaaaac aaatgaataa ggttacttaa agaggtgaat acgatgaaac tgatcaaact
      241 atgggaagca gatttaaaga aagcttatca gctacaaact agctttggac caaatgagag
      301 tggatttatc aacaatgctt atggctatac atttgctgaa tttttagatt atgtaaaaaa
      361 atgcgaaaaa acgtctttag gaatcgatct tcctgagagt tacgttcccg caaccgtctt
      421 tattttagaa agtgatgaac atgagtatgt aggaattttt aatttgcgcc ataaactgaa
      481 cgaaacatta agaaacggcg ctggccatat tggttacggc atctccccta attatcgtaa
      541 aaaaggatat gccacacaag gattagcatt agtactacaa gaggcaaaaa aaatcggcat
      601 tgatgaggca cttttgtctg taaataaaaa aaatcttggc agtcttaaag cacaacaaaa
      661 aaatggggct gcgatttatc gtgaagatga gaaagaatac tatacgaaaa tcaaactgat
      721 gtaaaaaaga aggattccaa aaggaaaggg atttgaatgt cctttatcaa tagaacacat
      781 ttatttaatt aacttataat atagcagaca aaattttata aacaaagaaa ccttttatct
      841 cttttcttct agaaaggata aaaggctttt ttgtttttcc tatttgcttc tcatagctag
      901 tcagccaaga tttctatcgt atccccaacg caatccgtgc ataacggctc atcttatctg
      961 tcgtccatgc tggataccag actagtttga cttctgcttt cgttacttct ggcacttctt
     1021 ttaatgcgtc atggatagat tctgttaaaa tatctgctaa tggacagccc atagttgtta
     1081 gagtcatctt aatgactgtt tctccatttt caggattgaa ttctatatca taaatcaagc
     1141 ctaaattgac gatgtcgatt cctaattcag gatcaatcac catttccaag gcagcaagaa
     1201 tacgttcttt gattagttct atttcttgtc cagaccgttc ttggtttgct tcgctcatcg
     1261 tacgacactc cttaacacga tttttattta tcatactctt tttttagctg cttgtaaatg
     1321 agaatttaga agtaagattc tcgtttaaat ttataaaaat gttcttatta gatgtaatag
     1381 attaaattta ttctaaaata aacctgattc ctcatttttt ttgtgaacga ttttttattt
     1441 ctggcaatac gccagttgtt cgatacattt tttgttcgtt ttcatttgca aaccggccaa
     1501 atactgaatg agttggtaaa atattgataa atgcatcgtc gtcgatttca tgaacgatct
     1561 gatcaagatc atacatttcg tagcgggtga tgaccatcat gatcatgttc ccttgttttt
     1621 ttgaatagcc accgacagac ggcagtaatg tcattccacg taccatccgt tttgctagtt
     1681 cttctgttac ctcttctggc ttgacggtaa cgatcattgc tgttactttt tgatgactcg
     1741 tgtggatggc atcaattact tgagtcattg cataaatgga aataatcgta tacaaggcac
     1801 tttcccagtt aaagaagaag cctgcgatga ctacaatgat accatttaga atcagcatgt
     1861 agtttccgac tgtttttcca gttgtttgtg aaagtacaag agagatgata tccatcccac
     1921 cagttgtaaa gcccattttt aaagataatc ctacgccaag acctaataaa acaccgccta
     1981 ctagggcatt catcaaaata ttatcagtga tctgtccgac tggaagcagc atcgtcatca
     2041 ccgatacaca tacaacattc acaaaactaa ggatcgttgc tcgttttcct actttgagaa
     2101 accctaatac aaacacaggg atattcaaaa gaaaaataaa tgttcctgtg tctatcttga
     2161 tatgaaacca ttcatataaa agcgaggaaa tgatttgagc gatcccattc atccctgcag
     2221 aaaagacatt ggctggaatc aaaaaataat ttaaagcgac cgcagcggtc aatccggtta
     2281 tcaaaataac cactattttc tttaatcttt catttttatg atacgcagtc ataattttac
     2341 tcacaagcac ctctccccta ctttggtatt ccaaatgtaa gaataacagt ttatctatta
     2401 aaataaaata cttcgaactc aaatgagaag aattttagca aacaggtaag aatctgccct
     2461 aatcctatga taagatagat gtatggtaaa tatttatatg tatatcagga gggttcctat
     2521 ggcaaaagat gctagcttcg acattgtatc agaagtcaat acagaagaag taaaaaatgc
     2581 gattcagcaa acaacaaaag aattgtccaa tcgctttgac ttcaaggatt ctacagtaga
     2641 aatcaaatta gagaacagca atctagttgt attgagcgat gatgatttca aaatcgaaca
     2701 gatcaaagat gtattatttg gtaagctgat caaaagaaat gtgccaatca aaaatatcca
     2761 tttttccgaa tcagaacacg ctctaggtgg caaagctcga caaactgccg atttggtcaa
     2821 cggaatcgat cgtgaaaacg cgaagaaaat cactacagct atcaaaaatg aaaaactgaa
     2881 agtaaaagca caaatccaag aagatcagat tagagtgaca ggtaagaacc gtgatgatct
     2941 acaagctgtg attgctttat tacgtaaact tgacttacca atcgagttgc aattcactaa
     3001 ctatcgttaa tcaaaaaacg aggctgacgt ctgtcattcc tcgttttttt gatttactgc
     3061 ttcttttgat aaccaaaaac gctgtattcc ttcttccaat tccccaacac ttcgtacttc
     3121 tcttgctgtt tgccaatgtt gtgggaataa acgtcgataa gaaaaactag aaaatcgttg
     3181 atctgccaat acaacgattc cttgatccgc catgccacga atcactcgac caccagcttg
     3241 caagactttg ttcattccag gaagctgata agcgtaagca aatccaaggt gttccttctc
     3301 atcataatat gatttgatca gttcctgctc atgattcatc tgcggcaaac ctacacctac
     3361 gatcatactg ccgatcaatc ggtcccctct caaatcgatt ccttctgaaa aaatcccgcc
     3421 aagtacgcaa aatccaatca atgtttcagc gggatttgtt tgaaattttg ttaaaaatcg
     3481 ttcgcgttct tcctcattca tgtccgtttc ttggattagc acattgctgt cgggatattg
     3541 ctgtctgaat gcttccaccg caagatctaa atattgataa gaaggaaaga aaacgaaata
     3601 attcccgacc tttccatcag caaaagcatg aatcgtctct actaattttg ccagactgtg
     3661 ttcccttttt cgataagtcg tctctaggta atttgcgatg aaaacttgct ggtttccttc
     3721 tgggaaagga ctcggcagtt tgtaagccag acttttttgt ccccccagtg tttcctgata
     3781 ataagagagt ggtgaaaaac tagcagaaaa aagaatactg ctttttcctt tatccaacga
     3841 ctgctcaaga aacaagcttg gatcaataca aaactctttt actatcagat cgcgatacgt
     3901 tttttcgact gttgtttcgt agtgatcatc ataaaattca ctgaccttca aaaaatgaag
     3961 caaatcaaaa taataaggaa gcaattgttc ttgtgatggg tgctcaggaa attctgccag
     4021 ccattcttta attttttcgc ttaattgata acctgccttt actaaagatt cagccggtgc
     4081 tttttgatga tgataatccc aatgatcttc ttttgctatt tctcggatcg aatcaaattc
     4141 ttttaatagt ttgttgaaac ggcgatgaag ttttttgaat tcctttggta tagcttcctt
     4201 acttcgtttc gttttttcat aagatagttc tgccgaatac atctctcggg aacgattcac
     4261 caaattatgt gcttcgtcaa tcaaaaacag atagtcctcg tttttttcat cttcaaaaaa
     4321 acgtcttaaa taaacagttg gatcaaataa ataattgtaa tccccaatga ttacgtcaca
     4381 ccagatactg acatctaatg atagttcgaa aggacaaagt gtatgctttc ttgcataagt
     4441 ttcgatgact tcacgagtga tctgattctc atgattcaaa agatcccata aaccttcatt
     4501 aatccgattg taataaccat tggcatatgg gcactgatct ggattacagg tcgtctcatc
     4561 taaaaagcag atcttgtctt tcgccgtcaa agtgacactt tttacttcag ctcctgtatc
     4621 ttttaatgct gtcatagcat cttcagctac ttgtctagtt attgttttag ccgttagata
     4681 aaagatcttt tctccctctt cttcccctac agctttcaaa gcggggaata acgtagagat
     4741 tgtttttcct gttccggtag gtgcttctac aaacaatttc tgtttcgtac ggatcgtttt
     4801 ataagcagct accgctagtt cacgctgacc tttcctatac tcatcatggg ggaatttcaa
     4861 agccatcaaa gaggcattgc gtacagtgcg ccagttttct tgaaaaacaa gccattcttt
     4921 ataatcttca gtcagctttt taaaaaaagt ttctaactgc tctcttgtct gatgttcaac
     4981 ctttctcgtg atcacttctt ctgccgtctg gaagtaagtc aattgcagat ttatttcttg
     5041 taaatcatgt tcttgactat agatataggc atacaccctt gcttgatgaa agaaaagttc
     5101 cacttgttct ggttctaaat cttcaaatcg tggttcggag gttttgattt catctatata
     5161 ataaataccg tctcttttga aaataccgtc tgcacggcct tcaacgatca ggtaatattc
     5221 atctaattga actgttgttt ttaaaaagac ttctttttca tagttttctc ccgcatcttt
     5281 ttggagcttg cggtggattc tcgctccttc taatgcagta tggttacttt ttttacgatt
     5341 gtcgatactt ccacgtcgca aaataaattc tactaagtgc cgcacagata tttttgactg
     5401 tttgatcatt tccaccacct ctttctctct aagattacct gaaaaaacct agtttagcaa
     5461 aaagagctct ctttccaaac ataaaaaaac accctgctga ttaggcaggg atataggata
     5521 tagaatttgg atttttggat gaatctaatg taacaaggac atattaaaat gctatgaaaa
     5581 cttactttcc ttctctttag aatatccaaa tacttttcat atagtctgat aattcagata
     5641 agtaaagaga aataagtacc tgtttttgtt tcctagcaat gaatgcgtta tactaatgga
     5701 tgaaagaagt tttggctatc aaggaggaat gcacatgcat atcgaaagat taaaaacagg
     5761 aatgattgaa gaaaactgtt atttggtcta caacgaagaa gcacttttga tcattgaccc
     5821 tggagaagat gcggaacgaa tcaaacaaga aatcaaaaaa acaaaccaac agccggtagc
     5881 gattttgctt acccatactc actacgatca tatcggtgca gtggaacctt tacgcgcgta
     5941 ttatcaaatc ccagtttatg tcagcccact agagcaaaaa tggctaggtg atccgatttt
     6001 gaatctttct ggactcggta gacatgatga tattgctgat gttattgtct cgcctgcaga
     6061 atatgaattt gaaatgaaaa cttatacttt aggaaacatg tcttttcgcg tcgtccctac
     6121 cccagggcat tctataggaa gcgtcagctt tatttttgat gattttgtcg tttctggcga
     6181 cgccctgttt agaggaagta tcggtagaac cgatctgtat acagggaatt tagaacaatt
     6241 acttcatagt attcggaccc agctgtttgt attacctgat gaatttgcag tctatccagg
     6301 acatggcgat gcaacgacga ttgaacagga aaaaaggaca aatccttttt tcaatcgtta
     6361 aaacgactta ggaggagaat aaaatgacag aaactacact ttatatcgta cgacacggaa
     6421 aaacgatgtt caatacaatt gaacgagtac aaggctggtg tgatacaccc ctcacaaagc
     6481 aaggacaaga aggcattcat tatttaggtc gcgggttaaa aaatgtcgac ttttcatttg
     6541 cttattctag tgacagtggt cgtgcaatcg aaactgcccg tattattcta ggagaacatt
     6601 ctaagggaaa agaaattcct tattttattg aaaaaaggat tagagaatgg tgtttcgggt
     6661 ctcttgaagg tggatatgac atggaaatgt ggggtgtgat ccctcgtgta ttgaatttta
     6721 aaacatatga tgaaatgttt accacagaag tttcttttga acaaattgct aatgctgtat
     6781 acaacttaga taccgctggg tgggcggaaa cttatgaaac attgcgagac cgtgtatgga
     6841 caggatttga agatatcgcc catcactgcg aaaaaaatgg tggtggaaat gttttagtcg
     6901 tatctcatgg tttgacgatc gcctttttgc ttaatctgat cgatcctaaa cagcctgttc
     6961 gtgctgggct tttaaatggc agcgtaacaa aggtcactta tgcaaacggt cgattttcta
     7021 tccacggcat caatgatacg tcttatatcg ataacgggaa aacacatagt cgcgactcgt
     7081 ttctataatg gggatatttt aatttttacc agagaatcat aaaaggcaaa gtctgaacga
     7141 gggctttgtc ttcttttttc tcttcgtgta cttatctgct ttatttctca agtttttctt
     7201 aatattaaaa aacagattgg gtttctcaaa aaataacggt atactataag atgtatctta
     7261 attgggaagg aaggaattta tgttttttgc aaatattatc aaagcagtga ttctcggtat
     7321 tattgaagga ataacggaat ggctgcccat cagtagtact ggtcatttga tcctagcaga
     7381 tgaattcatc aaactggatg ccagttcaca atttatgtcg atgttcaatg tcgttatcca
     7441 attaggggcg atcttggctg tcgtcgttct ttattttcac aagttaaacc cattttctcc
     7501 aagtaaatca acggtagaaa aaaaagatac gtggacactt tggtttaaag tgatcgttgc
     7561 ttgcatacca gccatcatca ttggtttgcc tttggatgat tggttagaag caaatttcca
     7621 caagttctta ccagttgcca ttgttttgat cgtttacggt attgccttta ttattgtcga
     7681 aaaacgcaac aaaaacaaaa cacctcgttg gacttctctg aatgacttta cgtaccaagc
     7741 agctcttctt gttgggttat tccaagtctt ggcacttgtt cccggaacat cccgttctgg
     7801 cgcaacgatc ctaggatcta tcttgattgg tgcttcgcgt tttgtcgcta cagagttttc
     7861 atttttctta gggattcctg tcatgtttgg cgcaagcggc ttgaagatcg tcaaatattt
     7921 agctgaaggg aattcattca aaatggaaga aactgttgtt cttcttgtag gaacattcgt
     7981 atcttttgtg gtttccgtct tagctatcaa attcttacta agctatttga agaaaaacga
     8041 ctttaccgct tttggctggt atcgtattat ccttggtatc attttgattg gttattggtt
     8101 tattgcaatg tagaaaaaac cagaacactt ctcttatttg agaaatgttc tggtttttta
     8161 ttttctttaa taataatcat tataaataat aaaaacaaca tcaagcttaa tcaccaaatg
     8221 ataaccatta tcgttctcat ttagctgtta ttattatcaa ttacattttt attatgctat
     8281 ccttgttttg acaaggaggt ctaataacga tgactaatca gaaaaaagct tttgcagcaa
     8341 caatcggctg ccttttattt atgatcttcg gttttatctt cgaccgattc tctcttacca
     8401 tcaccccact tttttatatc agtgccatta tcatcggtgg atataaacaa acatcggaag
     8461 gcttgaaaga attgtggaat gatcggacac tgaatgtgga tttattgatg gctttagctg
     8521 ctattggtgc ctgtttgatc ggtaattact ttgaaggagc aatgttgaca ttcatctttt
     8581 gtttaagcgg tgcattagag gaatacacaa ctaataaaag tcaaaaagaa attactgctt
     8641 tgatgaatct tcaaccacaa aaagcccaat tattaaaaga aaatggacaa atgcaagaag
     8701 tagatgtttc tcaactaaag gtggataact tggtatttgt cgctaaagga gcagctgttc
     8761 cgatagatgg ctttttagaa agcacacagg caacgatcga tgagtctgca ttaagcggtg
     8821 aatctgtgcc tgtagaaaaa tatttaggcg atgagatgta tgcaggaacg ctgaatcaag
     8881 gaaatcccat cacgattcga gtaacaaaaa cgagttccga aactgtcttt gctaagatta
     8941 tccaattagt agaagaagcg cagaatacgc caactcaaac agcttcattt atcgaacgaa
     9001 tcgaaaataa ttatgtaaaa ttgattttgc ttgctgttcc tctaatgatc cttctccccc
     9061 actttttcct aggttggagt tgggatgaaa gcttttatcg aggaatggta ttattagttg
     9121 tggcttctcc ttgtgcgctt gtcgcatctg ctacaccagc ttctctagct gctctttcaa
     9181 attctgcaaa aaatgggatt ttaatcaaag gcggtatcca tttagaaaag ctttctgaat
     9241 taaaagcgat tgcctttgat aaaacaggaa cgctcactaa aggaaaacca gttgtaactg
     9301 acagcttttt ctttgatgaa aaggatttgg cacaacaact tcttgttgct atggaacaaa
     9361 aaacgacgca tccacttgct catgcgattt tgcaatattt cgattgcaca atacctgaag
     9421 aaatccaata cttaccaata gaagaaatta ctggttttgg attacaaact acctatttag
     9481 gtgcgcaatg gaaagttggc aagcacgcat atgatcctga gacgatgata atcagcaaag
     9541 agattgcgga aatgatcgaa cgtttggaaa atcaaggcaa aaccgtgatt tatctttcta
     9601 aagatcagca gttaattgct gttttaggtc tgctagatat tccaaaagca aatacccaac
     9661 aagtaatttc ctactttaaa tcccaaaaca tccatacaag tatgataacc ggcgaccatt
     9721 ctggcacagc aaaggccatt gctgaacaag tagggattga acattactat gcaagctgta
     9781 ctccggaaga aaaaacacaa ctagtcaaaa aagagaatga actttaccaa gtaaatgcga
     9841 tggttggtga tggagtcaat gatgcgcctg ctttagcagc cgcttcttta ggtgtcgcta
     9901 tggggcaagg gacagatgcc gctatggata tcgcagacat cgtcttaatg aaaagcgact
     9961 taaataaatt tgtctacagt catcggctat ctttaaaaat gcggcggatc atcaaacaaa
    10021 atattatttt ttcgattctt gttattctct tgttgattgc ttctaacttc ctgcaatttt
    10081 taaatctacc tcttggagtt gtaggacacg aaggaagcac aatattggtt attctgaatg
    10141 gcttgcgttt actaagacct ttacctgatc tttctgattc ttcaaaaaaa tgtaagtctt
    10201 gtccactata caaagcgcgg ctgtcttctg tcaaataagc aaagtgtttt tttatgattt
    10261 tgtttatttt atgaaaaaaa cagcttatga ataggtcttg aaggatgaat tcctactttt
    10321 gtaactttct aaaaacatgt tatgattgtt tcgaagaaag tgaggaatat atttcatgga
    10381 aagttggctt tttttaggaa ttattttact tgtagcaatt ttagctaaaa atcaaagtct
    10441 gatgatagcg gcagcttttg ttttgattat gaagctcata ccagcctccc gttccttttt
    10501 cccttggctt caaggcaaag gaatcaatat tggggtgacg atcatcacag cggctattct
    10561 aatccctatc gcaacaggag aaatcggtct acgtgatcta ttagaagcct ttaaatcccc
    10621 tattggctgg gtagctgttt tttgcggtgg gctagtggct gtcttatctt ccaagggtgt
    10681 aggattgatt gcttctgatc cacaaatcac tgttgcactt gttttcggca cgattttagg
    10741 tgttgtcttc ttaagaggaa tcgccgccgg accggtcatt gcttctggta tcacttactg
    10801 tatcttacaa gtatttgctt taggaaaata gaacaaattt tgtctatttt aggagattct
    10861 taacaccccc aatttactga tccgtgatat actgacaacg aggtgataca atgttcgaaa
    10921 aattaaagta tttcatagga acaatcggct ggtttcttct agcggtactt ttgattctgg
    10981 gcttagctta tctattattt cctgatttga tacaaaatgt gtttgatact gctttttgat
    11041 tgacctatta aaacgatata ggggatgctc ctgcgatgca ggggcttttt ttaatcaatg
    11101 ttttgtaata atttaggccg tgccaatgag acttcctata gaattcgatg ctcttttttc
    11161 tttttggctc ttctgctctt ttttgttata ctagattaca gtggacaaaa gaaagaatga
    11221 tcgcatccac ttcaacgaat atcaaaatgg aagaattagt gcctgttttc aggttacttt
    11281 gaaaagaaag gaagacgctt gtggaaactt ttgaaggtct tttaattgat acgtacgttt
    11341 tacaagaaca gccaatgatg ctgactggga tcaacttgca gcttgaggac cgtgtgatca
    11401 atggcctgct taaagatcgc tatttggcaa aatatatctt acgtttgccc ctaaataaat
    11461 atgtgctgga agtaagtggc aaatggaacc gtcggaacca gttggtcata aaaaaaatgt
    11521 tgataaaaaa tatcgatgaa tacattaaaa taatcggtac accagaatag cctttactga
    11581 tcaaaggata ctggttataa aataactgca gtttcttaat agaaaaacaa agaacaacag
    11641 ggaatcgtat cccttttcaa aaaaaacttt gcaaacggtt
//
LOCUS       DAXWLG010000075        11611 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXWLG010000075 DAXWLG010000000
VERSION     DAXWLG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.076"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(44..841)
                     /locus_tag="ACGE4T_001627"
     CDS             complement(44..841)
                     /locus_tag="ACGE4T_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFF8386902.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(838..2022)
                     /locus_tag="ACGE4T_001628"
     CDS             complement(838..2022)
                     /locus_tag="ACGE4T_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFF8386903.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2047..3294)
                     /locus_tag="ACGE4T_001629"
     CDS             complement(2047..3294)
                     /locus_tag="ACGE4T_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8386904.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE4T_001630"
     CDS             3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE4T_001630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFF8386905.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5274..5588
                     /locus_tag="ACGE4T_001631"
     CDS             5274..5588
                     /locus_tag="ACGE4T_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFF8386906.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5613..6209
                     /gene="recR"
                     /locus_tag="ACGE4T_001632"
     CDS             5613..6209
                     /gene="recR"
                     /locus_tag="ACGE4T_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFF8386907.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE4T_001633"
     CDS             6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE4T_001633"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFF8386908.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6932..7261
                     /locus_tag="ACGE4T_001634"
     CDS             6932..7261
                     /locus_tag="ACGE4T_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFF8386909.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7275..8213
                     /gene="holB"
                     /locus_tag="ACGE4T_001635"
     CDS             7275..8213
                     /gene="holB"
                     /locus_tag="ACGE4T_001635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFF8386910.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8249..9073
                     /locus_tag="ACGE4T_001636"
     CDS             8249..9073
                     /locus_tag="ACGE4T_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFF8386911.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9066..9413
                     /locus_tag="ACGE4T_001637"
     CDS             9066..9413
                     /locus_tag="ACGE4T_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFF8386912.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE4T_001638"
     CDS             9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE4T_001638"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFF8386913.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE4T_001639"
     CDS             10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE4T_001639"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFF8386914.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
BASE COUNT         3716 a         2174 c         2512 g         3209 t
ORIGIN      
        1 ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc
       61 caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag
      121 gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt
      181 tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca
      241 attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa
      301 cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt
      361 cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca
      421 ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta
      481 agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt
      541 agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg
      601 cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta
      661 cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt
      721 ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag
      781 tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaat tttttcatca
      841 tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt
      901 cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt
      961 cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac
     1021 cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt
     1081 caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc
     1141 agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc
     1201 ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc
     1261 gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag
     1321 cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg
     1381 ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc
     1441 ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt
     1501 gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc
     1561 gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac
     1621 gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc
     1681 tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc
     1741 cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc
     1801 ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct
     1861 ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc
     1921 tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa
     1981 cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta
     2041 aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg
     2101 gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg
     2161 ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc
     2221 tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg
     2281 tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga
     2341 accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt
     2401 ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct
     2461 aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt
     2521 gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga
     2581 atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga
     2641 aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc
     2701 agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc
     2761 gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa
     2821 gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt
     2881 gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc
     2941 gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat
     3001 ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat
     3061 ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc
     3121 tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt
     3181 gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca
     3241 ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa
     3301 ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata ccataggaag
     3361 atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata
     3421 cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata
     3481 gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg
     3541 ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc
     3601 tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa
     3661 gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga
     3721 gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat
     3781 cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc
     3841 aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct
     3901 ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat
     3961 attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca
     4021 gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt
     4081 gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga
     4141 aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac
     4201 aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag
     4261 ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat
     4321 tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga
     4381 cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag
     4441 tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat
     4501 tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt
     4561 cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt
     4621 tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat
     4681 caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac
     4741 aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct
     4801 gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat
     4861 gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc
     4921 tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga
     4981 agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga
     5041 tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc
     5101 acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt
     5161 gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt
     5221 tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc
     5281 gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg
     5341 cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg
     5401 tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg
     5461 ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga
     5521 tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag
     5581 gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac
     5641 tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct
     5701 tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg
     5761 taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg
     5821 aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag
     5881 atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg
     5941 tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc
     6001 ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag
     6061 aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc
     6121 tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga
     6181 aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat
     6241 ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg
     6301 ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag
     6361 tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga
     6421 tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc
     6481 tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag
     6541 ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc
     6601 gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc
     6661 cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa
     6721 acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac
     6781 gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa
     6841 gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc
     6901 aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga
     6961 caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact
     7021 atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga
     7081 tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt
     7141 tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga
     7201 agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta
     7261 ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc
     7321 agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg
     7381 agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc
     7441 aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag
     7501 aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca
     7561 gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga
     7621 tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag
     7681 agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta
     7741 ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc
     7801 atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa
     7861 acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact
     7921 tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc
     7981 aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct
     8041 tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag
     8101 tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca
     8161 atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg
     8221 caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga
     8281 tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt
     8341 attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat
     8401 tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga
     8461 attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga
     8521 aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg
     8581 aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa
     8641 agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga
     8701 agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt
     8761 aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc
     8821 aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga
     8881 atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg
     8941 taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg
     9001 acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga
     9061 gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa
     9121 atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata
     9181 caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg
     9241 cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa
     9301 aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg
     9361 acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa
     9421 caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca
     9481 gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc
     9541 gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc
     9601 cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat
     9661 ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat
     9721 tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat
     9781 cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc
     9841 tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc
     9901 atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa
     9961 gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac
    10021 attgacgaat ttcttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac
    10081 aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga
    10141 acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt
    10201 gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc
    10261 aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca
    10321 agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa
    10381 gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt
    10441 gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc
    10501 aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca
    10561 tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg
    10621 tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag
    10681 tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa
    10741 atacagcgat atttccaaac aagtacggga agtattctat cgttatacag atattgtgga
    10801 accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc
    10861 agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg
    10921 ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc
    10981 tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat
    11041 tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta
    11101 tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat
    11161 gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag
    11221 agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc
    11281 agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca
    11341 aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa
    11401 aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct
    11461 tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac
    11521 gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat
    11581 ataaaaacag ctttgagagt tgaaaccgga a
//
LOCUS       DAXWLG010000076        11610 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXWLG010000076 DAXWLG010000000
VERSION     DAXWLG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.077"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE4T_001640"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE4T_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8386915.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACGE4T_001641"
     CDS             complement(322..891)
                     /locus_tag="ACGE4T_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFF8386916.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACGE4T_001642"
     CDS             1077..1979
                     /locus_tag="ACGE4T_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8386917.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE4T_001643"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE4T_001643"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFF8386918.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE4T_001644"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE4T_001644"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFF8386919.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE4T_001645"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE4T_001645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFF8386920.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACGE4T_001646"
     CDS             8547..8732
                     /locus_tag="ACGE4T_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFF8386921.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACGE4T_001647"
     CDS             complement(8794..9990)
                     /locus_tag="ACGE4T_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8386922.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACGE4T_001648"
     CDS             complement(10160..11596)
                     /locus_tag="ACGE4T_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8386923.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3422 a         2480 c         2039 g         3669 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc
//
LOCUS       DAXWLG010000077        11446 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXWLG010000077 DAXWLG010000000
VERSION     DAXWLG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.078"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            67..840
                     /gene="sufC"
                     /locus_tag="ACGE4T_001649"
     CDS             67..840
                     /gene="sufC"
                     /locus_tag="ACGE4T_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFF8386924.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            853..2139
                     /gene="sufD"
                     /locus_tag="ACGE4T_001650"
     CDS             853..2139
                     /gene="sufD"
                     /locus_tag="ACGE4T_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFF8386925.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2136..3371
                     /locus_tag="ACGE4T_001651"
     CDS             2136..3371
                     /locus_tag="ACGE4T_001651"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFF8386926.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3358..3828
                     /gene="sufU"
                     /locus_tag="ACGE4T_001652"
     CDS             3358..3828
                     /gene="sufU"
                     /locus_tag="ACGE4T_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFF8386927.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3833..5224
                     /gene="sufB"
                     /locus_tag="ACGE4T_001653"
     CDS             3833..5224
                     /gene="sufB"
                     /locus_tag="ACGE4T_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFF8386928.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5319..6461)
                     /locus_tag="ACGE4T_001654"
     CDS             complement(5319..6461)
                     /locus_tag="ACGE4T_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8386929.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(6500..7234)
                     /locus_tag="ACGE4T_001655"
     CDS             complement(6500..7234)
                     /locus_tag="ACGE4T_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8386930.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            7466..7741
                     /locus_tag="ACGE4T_001656"
     CDS             7466..7741
                     /locus_tag="ACGE4T_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386931.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7839..9836
                     /locus_tag="ACGE4T_001657"
     CDS             7839..9836
                     /locus_tag="ACGE4T_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386932.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9912..10247)
                     /locus_tag="ACGE4T_001658"
     CDS             complement(9912..10247)
                     /locus_tag="ACGE4T_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8386933.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            10374..10589
                     /locus_tag="ACGE4T_001659"
     CDS             10374..10589
                     /locus_tag="ACGE4T_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8386934.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            10626..10988
                     /locus_tag="ACGE4T_001660"
     CDS             10626..10988
                     /locus_tag="ACGE4T_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFF8386935.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            11034..11360
                     /locus_tag="ACGE4T_001661"
     CDS             11034..11360
                     /locus_tag="ACGE4T_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386936.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         3789 a         1951 c         2326 g         3380 t
ORIGIN      
        1 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
       61 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
      121 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
      181 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
      241 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
      301 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
      361 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
      421 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
      481 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
      541 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
      601 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
      661 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
      721 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
      781 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
      841 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
      901 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
      961 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     1021 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     1081 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     1141 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     1201 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     1261 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     1321 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     1381 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     1441 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     1501 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     1561 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     1621 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     1681 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     1741 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     1801 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     1861 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     1921 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     1981 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     2041 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     2101 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     2161 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     2221 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     2281 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     2341 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     2401 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     2461 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     2521 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     2581 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
     2641 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
     2701 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
     2761 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
     2821 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
     2881 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
     2941 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
     3001 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
     3061 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
     3121 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
     3181 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
     3241 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
     3301 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
     3361 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
     3421 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
     3481 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
     3541 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
     3601 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
     3661 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
     3721 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
     3781 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
     3841 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
     3901 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
     3961 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
     4021 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
     4081 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
     4141 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
     4201 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
     4261 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
     4321 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
     4381 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
     4441 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
     4501 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
     4561 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
     4621 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
     4681 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
     4741 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
     4801 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
     4861 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
     4921 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
     4981 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
     5041 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
     5101 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
     5161 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
     5221 gtaaacctgt tataggagct agaacccttg atatataagg gttctagctt ttttgttttg
     5281 cctgtatact agaaatgacc agattttgac cagctctttt actgatgagc catcatatca
     5341 gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat
     5401 gtcactttac tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct
     5461 tctaataata aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta
     5521 tattttttca aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt
     5581 ttaactgttg taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac
     5641 tcttcttttt gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag
     5701 ataactcgtc tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct
     5761 acagcaagag ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt
     5821 aaagcgagta cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat
     5881 tgtttcatgt taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag
     5941 taaaatttac gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga
     6001 ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc
     6061 atataatcgt aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg
     6121 acagagatat tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat
     6181 cttactgaga ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca
     6241 ttatacaggt cttcaaatgt catatcaggt gctttctttt taacaccgta taatatttga
     6301 tgtctgtact cgtcataatc tttttgagct tcttgtttgc tactaaaacc tcttctcgta
     6361 gtatttattt tttcaccagt tcgtggatcg gtggcaatgt gtcctttata catccacgcc
     6421 acttgaccgt ttttcttttt gtactttgat atttttgcca ttaaatcatc tcctcttagt
     6481 ttacaaggag tacttaccac tagatggtat cacctccctt catttttttc ttagtgtatt
     6541 ttttcccaat tgatgaaatc gtttgttcta gcttgtttat attatcagta atttctctca
     6601 cagcttcttc gggataagac atgtctttca attgaatagg tagccattca tctaccattc
     6661 tttctagtag ttcttctata ctgctagtga ggaaaagaat gttattgtca aattcttcta
     6721 caatagcatt gatgaataat tcttctattt ttaagatatt gaaaagacta attttttcat
     6781 ttttaacttt attacaagtc ttgttagcaa taaattcttg catctctggt tctaatttgg
     6841 aatatctatc cagccatgta tctaaattag agtgtactgg tgggaactgt tgacgaagat
     6901 gctggaacag gtttttatct ggaaatgtta taccatctag tacaatgcta tctctataaa
     6961 gaataattcc caaaatatat tcttctacat tgccacaaac taactcctct atactcatat
     7021 ttcccaaatc agcaattcgg gataatcgct ctggtattgg taaataggtt cccttctccc
     7081 attcgtagat agtttttttg tcaattaatt gaccgttgtt aatcataaga ccaaatgctg
     7141 gttgtgttaa ttttttactc tttcttatag cttttatttt tgttccgagt atcttttttc
     7201 gttctaaata atttattggt ggacgtttta tcatattgac cacacctcct aaaattagta
     7261 tacctcaaaa aaggggtgtt gtaaataaat tttacaaagc tgttgacacc ccttttttat
     7321 cgaggtatta tgtgtatgtc gatgaactgg taatcatttt ttgtttgatt aatcggttaa
     7381 taacaaaata cttttatttt gagcaaaaaa tacgaagata attcttagaa aatttgttca
     7441 agcaatcacg aaaggagggg gaaatatgtc tctacaaatc attgatagca ttgtttctat
     7501 acaagttgaa caacaagtga aagagataat tacagtgcta aagcctatgc tagaagacat
     7561 tatttctgga gtcactctag agtatatgga ctatcagcag gcaagtgaat atctaggtgt
     7621 atcagtagga acgattagaa aatatgtttc tcaatacggg ttacctgtta taaaaattga
     7681 tacggtcatt agatttaaaa aatctgatat tgatagtttc atggaacggt ataaaggttg
     7741 agtaaacaaa taagactaaa acatatagta ataaagagtt tgtttttatt atctgtatgt
     7801 tttagtcttt aacagaattt tataaggagg aaacacaaat ggaaacatca atgattaata
     7861 ttccaattta tgtagatcaa gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa
     7921 ctgatgtaag agataggtgt ttagataact atagagaagt tacaagagat gagtttgtag
     7981 agatactaaa ttcatcacaa tcatttattc caagcaagac aaagaataaa ggcaatgaca
     8041 aggagagttt tgtggaaacc agagtaatta ttctggatgt cgataacact gttaaagatg
     8101 agaaggggaa ggtagtagat ttgagtaaag atgatagtag atacttaagc atagaaaagg
     8161 ttttatctat caatttagtt cataattctg cgtttgcgat acaaaaaagt attcggtact
     8221 ctgaaaatct agaaaaatac aagattgtct ttctattaaa agaagcaatt actgactatg
     8281 gtaaaatggt tgctgtttac gaatacttga aaaagaacat ccccgggtgt gatgacaatg
     8341 taaatgcttc gaatagaatg ttttttggag gatataagtc agatgcagtg ataataatta
     8401 atgaagacaa catgttagat attactgagc ttccgattga ttttaccatg acatgttcta
     8461 aaaattgtca aagtgattca gggagagagt atgaactagt caatgaaacc gattttgtta
     8521 agttgataaa aaatgacgac aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg
     8581 attcctccga catgtctttt aatgagattt atgaaagatt gttaaccatt gatatgaata
     8641 aattacttaa gtcaagtaaa aacttaagat gtctgtttca caacgatcat aatccctctg
     8701 ctagtatttt taccagtaaa actgggcatt ccatttttta ttgtcattcc agtaattgtg
     8761 atatcagtga aaattttatt ggagttgtga tgctaatttt aaagaaagac agtcgggtag
     8821 aaacatttaa ttggctatta gagaacctag acttgtatcc tacacatttt cgaaaattga
     8881 aagaggaatc aaaagtactt tttgacactc ttgaatcaat gaaagacaaa cttttcagga
     8941 caataaggaa ttatctagag gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta
     9001 atttttttga tgatagtaag gaaagtgtga cttgtatttt gtcgggggaa caattagcta
     9061 aaaaaatgag ttcttactat ggtaaaactt atgacataga taaatgtaat aagttactat
     9121 cacttatgac ttttatagga ttgataaaaa aattagatga tgaagatgtt cctttgaaga
     9181 tgctcgaata cctaaatagg ttaaaggaag agaaagcgtt aggaaacttt aatcataaga
     9241 gaagcaatgt ctatcgatta gacgttttta atgcaacttc tgtgattcaa aaattgaatg
     9301 aagatatcct tccacttctg gaaaaaattc attttacata tcaatatttt tcttatgaat
     9361 gggttaagat atgttttaac gaaaaagagg ctaaaagagt atttcctcaa aattcccgat
     9421 cagaactcag caaggaaaaa caattgattt tagatgaagc acatgaagtt ttacagggct
     9481 tatcgtggca agggacatat ctaatgacag agaaagaact tattaacact gtttgtgaac
     9541 gcctacgtaa tgttggggat aacagcgtga aaaatgcttt aataaaaaac agaggctact
     9601 ttgttaaaca aggattcact cttgaacgag cttctaagga tattaagaaa ctatacaatg
     9661 tgaaagcaag tagtagtttt ttaatatatt tagcgaaagg ggcagggata gatgaaacaa
     9721 taaaagaatt gatattatca ggagctggga tagttcctct gtatcaattt acaagtgtaa
     9781 caagagatga tggacggaga gtaaggaaga gaaaagaccc aattgtatta aactagacag
     9841 gttatccata gctcttaatc ttatttcatc tcaatacacc caccgttaaa gttcggtggg
     9901 ttatttattt ttcaatcttt caatagctga ttgcgttcat tttcttgcct ttttaatagc
     9961 ttcatatagt caattgttcg aactgcatct tcagtgctaa gagaacgata gagagccaaa
    10021 aactcttcct cataatctac gacaacattg tctagaaaat ggcttacagg ggttcctagg
    10081 gctctagaaa gagctgttaa gctatcgagg ttaggctctg ttacagaatt ttcccatctt
    10141 gctactgcct gttgtgtaga gaagattttt tcagctaatt ctttttgtgt ccacccgaga
    10201 gcttttcgct tttctctaat gacctttgca tattgtttct gtttcattaa tacaccccca
    10261 tctcagtata gtacaacaag ttgttgtatt tgtaatggta tttgaagttt tttatatttt
    10321 atgagttgaa atacatcata ttgttgtata taataaaaca ggaggggaga atcatgaaaa
    10381 aagtaaataa tctcagccga tacagaaaag aaaaaggact ttctcaaacg gagttagcaa
    10441 aaaaaatgaa tgttacacag caatgtgtaa gttcgtggca aacagggaga acgattccta
    10501 aaccctatca aatgaagatg ctttctgaga tattaagtgt accaatgaat gagctgtttt
    10561 ctgatgtatt taataaagta aacagttaac taataagcaa ttgaatgatt ttaggaggag
    10621 aagacatgta ttgtagaaaa tgtggcaaag aaataacaga tgaaagtaag tattgccagt
    10681 tttgtggagc actacaagaa acagatgaga caaccggaac agacacagta aagaaaagta
    10741 ctgagcagac aattaatgaa atgaaggaac aaatcgtaca tggacaagag acagggaagc
    10801 tgtatttgat tattggttgg atttctatgg tagtatcgtt agcatttatt cctgttgtat
    10861 ttggtgctgt tgctgttatt atgggctact tatatcgaga aaaagatgag aaaatgggta
    10921 cgattttgat gatagcaggg gtagctggag caatctttgg tgtactttta gggatgtcaa
    10981 tgtactgata agagggataa gaacaaatta ttttggagat aggagataaa aagttgaata
    11041 aagtaaatat gaacaagaag tttgtcatta taggtattgt tgtcttggct attataggta
    11101 ttattttttc aaaaatgaca ggtacatctg gattagagaa gtcatcaaaa ccattggtaa
    11161 cacagatttt acaagaacag tacaacttaa atcgatcatg tgatgatgtg acgattactc
    11221 atgaaaatgg agataacaca taccgggcta aagctatatt agacaatgga agtgctatca
    11281 acataaatat tgagtactat ccaaagaaag accgtatata tgtggagata ccatatgctg
    11341 aggtgttaat gttaaattga tttaaatgta ttatgcatac cgtccaaaca ggaactccca
    11401 aaggtgtaaa ttcactaggg agtttttata gtttcctata aattaa
//
LOCUS       DAXWLG010000078        11371 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXWLG010000078 DAXWLG010000000
VERSION     DAXWLG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.079"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            35..334
                     /locus_tag="ACGE4T_001662"
     CDS             35..334
                     /locus_tag="ACGE4T_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386937.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(431..865)
                     /locus_tag="ACGE4T_001663"
     CDS             complement(431..865)
                     /locus_tag="ACGE4T_001663"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8386938.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1097..1807)
                     /locus_tag="ACGE4T_001664"
     CDS             complement(1097..1807)
                     /locus_tag="ACGE4T_001664"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8386939.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1797..2657)
                     /locus_tag="ACGE4T_001665"
     CDS             complement(1797..2657)
                     /locus_tag="ACGE4T_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFF8386940.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2662..3300)
                     /locus_tag="ACGE4T_001666"
     CDS             complement(2662..3300)
                     /locus_tag="ACGE4T_001666"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFF8386941.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3315..3614)
                     /locus_tag="ACGE4T_001667"
     CDS             complement(3315..3614)
                     /locus_tag="ACGE4T_001667"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8386942.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3657..5141)
                     /locus_tag="ACGE4T_001668"
     CDS             complement(3657..5141)
                     /locus_tag="ACGE4T_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8386943.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5161..5622)
                     /locus_tag="ACGE4T_001669"
     CDS             complement(5161..5622)
                     /locus_tag="ACGE4T_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8386944.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5640..6707)
                     /gene="ulaG"
                     /locus_tag="ACGE4T_001670"
     CDS             complement(5640..6707)
                     /gene="ulaG"
                     /locus_tag="ACGE4T_001670"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFF8386945.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6925..7695
                     /locus_tag="ACGE4T_001671"
     CDS             6925..7695
                     /locus_tag="ACGE4T_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8386946.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7968..8795)
                     /locus_tag="ACGE4T_001672"
     CDS             complement(7968..8795)
                     /locus_tag="ACGE4T_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8386947.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9363..10598
                     /locus_tag="ACGE4T_001673"
     CDS             9363..10598
                     /locus_tag="ACGE4T_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8386948.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11130..>11371)
                     /locus_tag="ACGE4T_001674"
     CDS             complement(11130..>11371)
                     /locus_tag="ACGE4T_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386949.1"
                     /translation="WQKEGGKNGNFISNGFRISRSTTKVIKSNIDLGAYNHIVGAAVW
                     CMNGNMYDVSVYSNHETCIEIIVLGIAISHGERDF"
BASE COUNT         3510 a         2361 c         1793 g         3707 t
ORIGIN      
        1 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
       61 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      121 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      181 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      241 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      301 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      361 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      421 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
      481 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
      541 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
      601 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
      661 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
      721 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
      781 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
      841 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
      901 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
      961 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1021 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1081 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1141 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1201 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1261 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1321 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1381 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1441 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     1501 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     1561 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     1621 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     1681 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     1741 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     1801 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     1861 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     1921 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     1981 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2041 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2101 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2161 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2221 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2281 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2341 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2401 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     2461 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     2521 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     2581 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     2641 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     2701 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     2761 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     2821 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     2881 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     2941 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3001 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3061 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3121 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3181 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3241 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3301 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3361 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3421 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     3481 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     3541 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     3601 actaaaattc tcatcttaat ccctccatta tgagtgttgt cgaatcagat tttcttttat
     3661 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     3721 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     3781 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     3841 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     3901 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     3961 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4021 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4081 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4141 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4201 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4261 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4321 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4381 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4441 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     4501 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     4561 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     4621 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     4681 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     4741 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     4801 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     4861 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     4921 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     4981 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5041 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5101 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5161 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5221 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5281 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5341 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5401 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     5461 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     5521 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     5581 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     5641 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     5701 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     5761 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     5821 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     5881 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     5941 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6001 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6061 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6121 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6181 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6241 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6301 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6361 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6421 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     6481 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     6541 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     6601 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     6661 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     6721 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     6781 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     6841 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     6901 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     6961 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7021 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7081 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7141 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7201 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7261 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7321 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7381 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7441 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     7501 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     7561 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     7621 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     7681 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     7741 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     7801 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     7861 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     7921 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     7981 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8041 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8101 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8161 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8221 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8281 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8341 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8401 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     8461 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     8521 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     8581 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     8641 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     8701 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     8761 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     8821 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     8881 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     8941 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9001 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9061 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9121 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9181 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9241 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9301 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9361 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9421 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
     9481 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
     9541 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
     9601 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
     9661 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
     9721 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
     9781 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
     9841 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
     9901 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
     9961 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10021 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10081 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10141 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10201 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10261 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10321 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10381 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10441 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    10501 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    10561 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    10621 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    10681 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    10741 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    10801 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    10861 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    10921 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    10981 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11041 tccgatgtat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11101 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11161 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11221 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11281 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11341 tttccatttt ttcctccttc tttctgccaa a
//
LOCUS       DAXWLG010000079        11297 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXWLG010000079 DAXWLG010000000
VERSION     DAXWLG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.080"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..2069)
                     /locus_tag="ACGE4T_001675"
     CDS             complement(<1..2069)
                     /locus_tag="ACGE4T_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8386950.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHV"
     gene            complement(2211..2333)
                     /locus_tag="ACGE4T_001676"
     CDS             complement(2211..2333)
                     /locus_tag="ACGE4T_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386951.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            complement(2593..2952)
                     /locus_tag="ACGE4T_001677"
     CDS             complement(2593..2952)
                     /locus_tag="ACGE4T_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFF8386952.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(2982..3323)
                     /locus_tag="ACGE4T_001678"
     CDS             complement(2982..3323)
                     /locus_tag="ACGE4T_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386953.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3320..3994)
                     /locus_tag="ACGE4T_001679"
     CDS             complement(3320..3994)
                     /locus_tag="ACGE4T_001679"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8386954.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(4928..6226)
                     /gene="asnS"
                     /locus_tag="ACGE4T_001680"
     CDS             complement(4928..6226)
                     /gene="asnS"
                     /locus_tag="ACGE4T_001680"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFF8386955.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6266..7456)
                     /locus_tag="ACGE4T_001681"
     CDS             complement(6266..7456)
                     /locus_tag="ACGE4T_001681"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8386956.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7477..8004)
                     /locus_tag="ACGE4T_001682"
     CDS             complement(7477..8004)
                     /locus_tag="ACGE4T_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFF8386957.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8051..10840)
                     /locus_tag="ACGE4T_001683"
     CDS             complement(8051..10840)
                     /locus_tag="ACGE4T_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFF8386958.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(10987..11187)
                     /locus_tag="ACGE4T_001684"
     CDS             complement(10987..11187)
                     /locus_tag="ACGE4T_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8386959.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT         3093 a         2469 c         1990 g         3745 t
ORIGIN      
        1 tttacatgca agcttcgcat ggcattcaaa acagctaata ctgtcacccc aacatctgca
       61 aatactgcat ctcccattga tgcaaaacca aaagcaccta aggaaagtac caaaattttt
      121 atcccaatag caaagacaat gttttgtttg acgattttca atgttttctt tgctaaatgg
      181 attgcttctg caatcttcga tggttgatca ttcatgatga ctacatctgc tgcttcaatt
      241 gcagcatcgc ttcccaatcc acccattgcg attcctacat ctgctcttgc tagcaccggt
      301 gcatcgttga ttccatctcc gacaaatgcg gtctttttat tgttactttg taagatttcc
      361 tctaaccgct gcactttatc ttctggaagc aattcgctat atatcttgtc cacgcctact
      421 tgcttcccga tatgatcagc aatttttttc gagtcgcccg tcaacatgac tgtgtttttc
      481 acaccagcct gttttaattg aaccaatgcg tctacggcat cttctttcaa ggtatctgca
      541 ataacaagat aaccactgta actttgatcg attgctagat aaagcaatgt accgatttct
      601 tgacttgaag gaaaagagat ttcaaatctt tccatcaatt tgtgattacc tactaatact
      661 tgtttgccat caacttcagc taagatccct tcacctgcaa attcctggat attagctgtt
      721 ggtaaaagcg gacctgcgta gccttctaaa acagattgag caatcggatg tgtagaaaac
      781 tgttctgcac tcgcaatata ttgcatgaat atctttggat cgatttttgt atcgatcgtc
      841 tggatcgaaa agtcgccttt cgttaatgtg ccggttttat cgaataccac tgtctcggtt
      901 tgcgcaagca actccaagta atttgaacct tttactaata caccgatttt acttgctcct
      961 ccgataccgc caaagaaact aagtggcacg gaaatcacta atgcacatgg acatgagatc
     1021 actaaaaatg tcaatgctcg atagatccat tcagaaaacg ccgctccaga aacaacaagt
     1081 ggaggtaaaa cagctaataa aacggccaat cctacaacga ctggtgtgta atagcgcgcg
     1141 aatttagtaa tgaaattttc agctggtgct tttttactgc tggcattttc tactaattct
     1201 aataatttgc tgatagtaga attttcataa gatttttcta cttgaactaa aaatggttta
     1261 tttttattga taaatccgct taatactgtt tcaccaacat atacagattt agggactgat
     1321 tctcctgtaa gtgctgacgt atcgactaat gattctcctt cgatgatcgt tccatctaac
     1381 ggtacacgtt caccaggctt cacaagtaca aactgaccga tcagcacgtc ttcagggttc
     1441 acggtcatca atccttccgc cgtctgcacg tgagctgttt ctggacggat cgccatcaat
     1501 tcgcgaatcg attttcttga atgactgact gccaatcctt ggaaatactc gccgatttga
     1561 taaaatagca tcactgctac ggcttctgga tattcaccga tcaacatagc gccaatcgta
     1621 gcaacagaca taagaaaatt ctcatcaaag atttttcctt tgacgatatt catcactgcc
     1681 ttcttcacta catcatatcc tatcagcaga taaatcaata aataccctat caagcgaatt
     1741 agttcattaa aatcaaataa tagcaataaa gcgatacctg caagggaaaa taaaatccgg
     1801 aacagcgtat ttctctcttt ttcatttcca tgttcagata ctttttcctt atctatttct
     1861 gttactttga catctggttc taattccttc actacttttt ttgtttcttg ttcaatcgta
     1921 gccaaatgat cttcctccgc gtcaatcgtt agttttccag aagtgaagtt taccgttgct
     1981 tccttgactc cattgatttt ctgaactcct ttttcgattt tcattgcaca gttcgcacaa
     2041 tctaaacctt ctaatctgta acttttcatt ttaatgacct cctcgcctca acatatgaat
     2101 atctcttcat aactaattat aaatgaatgg ttattcatat gtcaaggtat ccgaaagaaa
     2161 aaatgaaaaa catctgcatc ggaaaatttc ttccatacag atgttttggc ttattctttc
     2221 tttttctctt cgatcattgc cactgctctt atccaacccg cacttgcctt cttgatcttg
     2281 acaggatagc aactaagttt gaagcctgtt gctggcaatt tgtctaaatt cattaatttt
     2341 tcgataggac aatatctttt ttcaatccct gcaaaatgtc cttcccagat atacgaagct
     2401 ccactacgct ttcaaaaata tagaaagagt tctttaatga aaattatttc aactttccat
     2461 aaacagtaac cccatataaa taaaagacgt aaaagtcaca tggaagaact tatacaggaa
     2521 aacgcataga gtcaggtgaa atacatgtat cccacctgac tctatgcttt atctaacctt
     2581 tattattctc ttttatacaa tttctgcacc gaatggcatc aacgccaatc gagcaagttt
     2641 gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga tgccgctgac
     2701 gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat taacgatcaa
     2761 tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag aaaggaaggc
     2821 gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc aaagtatccc
     2881 cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga tgacatttcc
     2941 taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg ggcaaaaatt
     3001 gattttattc ttcttttttt agcttccagc atcaaatatt ctgcaaattt tttttggatt
     3061 tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga tggtcgctga
     3121 cccacccgtt cttctgactg ttgcacgtcc gcttgcgcaa aaccgataat ccgtttttct
     3181 tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc cgtttttgct
     3241 tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtag atcttctttt
     3301 actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta tctccactaa
     3361 aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca tgtccgccaa
     3421 tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataagca atccctaaag
     3481 cattgaatat gccatctaaa ggaaccagca atgcgccata ttccttagca agtttacgaa
     3541 caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg gttataggaa
     3601 gtacaaacgg ctccatcaag atgatgttgc agattcccgc tttttgaagt tcatttagta
     3661 attttcgata aacggattca aattgctgag cagatttctc agttccgaat atagcctgat
     3721 cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag tcaggttgta
     3781 aagctaaaca atcttctcgc cagcgtactt gcagatctgc aattttgtct cctcctatcc
     3841 cacggttcat gcattcaaat tccatcgttg gatattggga gattagtttt ccggcaatca
     3901 aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct gcgtctgtaa
     3961 tgctgtcacc gataaataat atacgttttt tcatttgtca aaaactcctt tttgaataaa
     4021 acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag agaaaatata
     4081 tgtataaaaa atgacaccag cctagtccct tcaaacaatt gaatgaacgc tcatcaagtg
     4141 tctagttttt tatgctattt ttttcttaac gtgctggatt ccccttgatt ccttcattgt
     4201 ttggtccaga gagatcgatc acacctaagc ttccgtcaat cagaccactt gatgacgcca
     4261 tcagaacata tatttatagg ctcatctaag acttccagta aatgttcctt tactaatatc
     4321 tcttctagat tgcttgggat agattggcca taactttatc tggtccgatg atttcaaggg
     4381 tgaattgaac ttacatatgg atcattgcat gctgaaaata cggacctact ccatcttcga
     4441 atgttactat tagtttgttt tcaggtgcta gatgctgctt tatttcaaca gcagaagcat
     4501 catcaaatca gatttccatt tgaataacct tctttgtata tgtttgattg attgcgattt
     4561 cgttatagaa ccttgtgaat caaatagcaa tttcttttgc tttcttctct tatctgctgg
     4621 aaacttcctt tatcccgtat ttaaaaaaaa agaactgcga gagttcgcag ttcttttttt
     4681 taaataaacg ttgctctccg gccaactcct tggtcacaag tcacaatagt catcaaacat
     4741 gaaaaaatgt ttttttgaaa ttactccttg tgactcggag ctgaacggca gacgcacaac
     4801 ctcttgatat aaacggtgtt caatcagctg accctcgata ttagctcaaa aaacagtaaa
     4861 atatgagaag ctatttttcc gttttgtttc tctaatactc aggtcaaggc actgatttca
     4921 caacctctta tggataaatt cggttcaaca aacgtgggaa tggaatagct tcacgcacat
     4981 ggtcgatacc agcaatccaa gtgactgttc gttcaaggcc tagaccaaaa ccagagtgcg
     5041 gaacagaacc gtatttacgt agatcaagat accatgaata ttctttttca tccaaaccgt
     5101 gattacggat ttgttctagc aagaaatcat agtctgttgc ccgttcgctt ccaccaataa
     5161 tttcaccgta accttctggt gcgatcatat ctgcacagat caccacgtct tctcttgttg
     5221 gatgtggttt catgtagaat ggcttgatgg cttttggata gtttaggata aagacaggct
     5281 gttcgaatga attcgcaatg aacgtttcat gaggtgaacc aaagtcttcg ccccattcga
     5341 tgtcatcaaa tccatttttc ttcagtaatt cgatcgcatc atcataagta atacgaggga
     5401 atggcaattt tgtataagtt tttaagactt ctttgtctcg tcctagtaca tctaatgcgt
     5461 attcgcaatg atctaaaaca ttttgtacta agaatgctac atattgttcc tgtacttcta
     5521 aactttcttc ttgatgagtg aaagccattt ctggttcgat catccagaat tcgatcaaat
     5581 gacgtcgtgt ttttgatttt tcagcacgga aagtcggacc gaatgaaaag acttttccga
     5641 aagccatagc agcagcttcc atgtataatt ggccactttg agataaataa gctttttgat
     5701 caaagtaatt tgtttcgaat agatccgtag tgccttctgg tgcagaacca gtcaagatcg
     5761 gcggatcgat tttgacaaaa tgatgttgat taaagaattc atacgttgca cgaatgattt
     5821 catttctgat ctgcataatc gcatgttgtc ttgaagaacg cagccataaa tgacgatgat
     5881 ccatcaagaa gtctgttccg tgttcttttg gtgtgatcgg ataatcgtgg ctctcgctta
     5941 ccacttccac atttgtaatt ccgatttcat aaccaaattt tgaacgactg tcttcacgga
     6001 tttctccagt tactaacacg gatgtttctt ggtgtagatt tttagctgtt tggaaaactt
     6061 cttccggcac ttcgcttttt acaactacac cttggaaaaa ggctgttccg tctcttaatt
     6121 gcaaaaatgc gatttttccg cttgatcgct tatttgcgac ccaagcgccg attgttactg
     6181 tttctcccac atggtttttc gaatcgatga tttgaatttg ttccacgtat gactctcctt
     6241 ttatattcct tgaatttctt caggattaat tttgactttt actctcaata aattgcttga
     6301 ttctgcgtac cgcttcttct aacgtagcgc gatcagtggc ataactcatg cggacatttt
     6361 ctggtgcccc aaagccttcg ccagtcacaa gcgctacccc tgtttcttct aaaagatctt
     6421 ctacccattg tgtgacattt tcatatttac acatgcgtag cgtttctttt acattaggaa
     6481 aaagataaaa agctccttgt ggcttttcaa gagaaacacc cggcagttct gccacaagcg
     6541 gatataacgt attcaggcgt tcttcaaagg cgttccgcat ttcttccact gtatcttgct
     6601 cgcctttcaa tgcttcaaca gctgcatatt gactgactgc tgtcggatta ctggttgact
     6661 gggaagcaac agcaatcatt ccattgatga tctcagaatt ccccaccgca taaccaatgc
     6721 gccagcctgt catcgcatat gttttagaca cgccattgat gatgatggtc tgatttttga
     6781 ttgcttctga aatcgttgca attggagtaa actcattgcc attataaact aaacggccat
     6841 aaatatcatc agcaactatc aaaatatcat tttttactgc ccattcaccg attgcctgca
     6901 gttcattctt gctataaatc attccagtcg gattcgatgg agaattgatg attacggctt
     6961 ttgttttttc tgaacgtgct tgttctagct gctcaacagt aactttccag ctattggatt
     7021 cttccccttt cacaaagaca ggaagtcctt ctgccagttt tacttgctca ccgtaactga
     7081 cccagtaagg cacaggaatg atgacttcat cttgcgggtc aaggatcgtt tggaaaaggg
     7141 tatacaaagc aaatttcgct ccgtctgtta cgactgtttg tgaaggttca tagacaagtc
     7201 catagttttt cttcagataa tcaacgattg cctgtcgaag ctctgggatt cctgctgttg
     7261 gtgtataata gctagctttc ccattgcgga ttgcttcgat tgctgcttct tggatgtttt
     7321 ctggtgtcgc aaaatccggt tctcccacag tcaaactcag cacatctctg ccttttgctt
     7381 ttaatgcctt ggcctttgcc gcagctgcca aagtgacaga cggttctaat cgttttgttc
     7441 tttttgataa ctccatccca ttcattcctt taccttttaa attcccttga ttgtcttgac
     7501 ttcatctcca gattcaaagc caatcaggta ataattcagt ttcccatttg tttcttttcc
     7561 tactacttcc cacacaggct gatcatcata catccctaaa gcagcttttt cgatttggat
     7621 ttcaggatgg gccgcagcaa ttgtctgttt tgcttcgttt tcagtcagtc cctttgtttg
     7681 atccaatact tttacttttt caccagattt aggaatgata acggcgattt cttttccttt
     7741 atcattcgta cccgtcaatg aaaagtacgt tttctttcga gtaaaccagt aaaaatgatc
     7801 ggcttcctcg agatttgcat atttctttgc gatttctact gcttcttttt tcgcctggac
     7861 cgccggacga ctggaacgca agtaaaaaat cgagctgatc acgatgaccg ctaataaaaa
     7921 tacgatcaag ccaagcaaca cgcgattaat cgttttttcc tttgtctgtt ttttcttcaa
     7981 gggtcgtcgc tcctttatct tcattccttt tcattatagc agattttaat gatgaaaaaa
     8041 gacggcacct ttaacttcct tcttcatttt tctttaagaa ttcatggata tcctgataga
     8101 tttcatctat agctagctct tttatcggta cttttttcgg taatgcatgg atgattcgtg
     8161 caccatattt tgcagtgact aatctccgat ccaaaataag taatacgcca cgatctgtct
     8221 ccgtccttat caaacgacct aatgcttggc gtaacttcaa agctgcttta ggcacggatt
     8281 cctgataaaa aggattgatc ccctctgatt ccagataagc attcctagct ttgaccatcg
     8341 gacgtaacgg gttgtcgaag ggtaaacgtg tgactacaac aatctgcaaa gcatctccag
     8401 gtaaatctac gccttcccaa aagctgtcag caccaaataa gacgcttttt tctgacaaaa
     8461 tgaagcgttt caaaagtttt tcccgactcc cgccaaatcc ttgagcaaga atttcacgtc
     8521 ctttttctag catagacaaa tgcattttct gatatacctt ttgcaaaaca tcatgagaag
     8581 tgaataaaac aaggatcggc cgatcttctt gttcaatcaa tcctgtgagt gctgcttcta
     8641 aataatcgat atatgcttga ttgctgactt gatggactgc aggtgcttct ttcggcacat
     8701 agattcttgc ctgtttctca taatcataag gagaagcaat cactttaaga ggtgctgctt
     8761 caatgccaag tcgtttagag agatactgtc gattaggtcc gatcttcagt gttcctccaa
     8821 tataaagaat atggtcatat cgaggatacc acacagttga cggcaacata gctgcttgaa
     8881 aatcaaggag atgaaaaatc atctgctgtg ctgtattccc gggaacgaac caatggatca
     8941 aatgatctcg ccagtcttct agccattggt ccatcagcaa gtattgcttg tctaattgat
     9001 caaataagct atataaactt gctaaatcga tccgcatctg gttggtccaa ctttcttgga
     9061 taatctcaaa agtcaagcgc atctgtgatt gtagttgttg gatctcttga taaaaaagtt
     9121 gcagatgatc cattgctgtt ccagattcca gtggcagctg atccaacatt tcttttgtca
     9181 tcatgacttc ttctggatat tgtccgttag gttgcttgct gcttactaag ttcattaatc
     9241 cctctgccac gcaaatctgc gcttcgcata gtgcagagag ttcttctcga taaatcgaca
     9301 accagcgcaa ggcttcatgt tccttcggca aataactttg tatgcggtca aataactgat
     9361 tcggctcttc cagttgctga atttgtttat ggaagtagtt aatacttagt cgtttgtcat
     9421 ttactttttc agcaatttcc ggcaaatgat gcacttcatc aatcaacaaa taaggactag
     9481 ctggaagcaa tggttctttt cgctgtgttt cttgtactaa aaatgcatgg ttgactatca
     9541 aaaaattgct ttgtttgatt ttcttttgca agaaacgcac aaagtcttgt ttgtaaaatg
     9601 actgattcct tgctaaaaac ccagtgcctc tgtgcgcgac atctgcaaag aatgcatggt
     9661 tcagattggt taaatgtaat tcatccaaat caccggtctt cgtttgtgtt agccaaacta
     9721 agacagccat ttggtacaaa gcatattgtt tttgtcccgg cggttccaca agtgtacctt
     9781 taaaacgctg taaatcaata tagtggcgat agcttttgat aatcacaggt tgtatcggct
     9841 gatctagcaa ttgattcagt aatggaatgt ctttttccat gatctgctgc tgcaaaacaa
     9901 gcgaaacggt gctgatgaca gctggttttt caggtgtagc caaataactc aaaggcaaca
     9961 gataaccaat cgttttgccc attcctgtag ctgcttcgat catcaaatct tttgattcat
    10021 cttttgtgaa atggtcgtag acagcattca tcagcctgtt ctgttctttt cgatacatca
    10081 gtgttttacc aaacattttt tcttttgctt gtttttttct tggatatgtc cgctcgccat
    10141 aatggacgga tgtgaatagc tccacttctt ttttctgcaa agctagccca tcgatgattt
    10201 ctaatgagag atcaaggggc tccgggtttt ctttcatttc ttcaagcacg tggtaaatga
    10261 aacgactcgt atccattcct gtatggctac ttaatcgtgc aatctgctct aatgtgatga
    10321 tcggcaattt cctcattcgt tcttcgatca gtaaaagaag ctgacctgtc acttcggcat
    10381 cactatccgc ctgatgagga ttctcatgaa taaagccaag gctttctgac aaatcagcta
    10441 agcgaaaact tggttccgtc ggcaagaaaa tttgtgcaag ttctacggta tcgatgcccg
    10501 gtatcttcaa actaggaagt ccgcatcgca tgagttcatg gttcaaaaaa ttataatcaa
    10561 aataaatatt atgcgcgaca aatatggtgt ctgccaaaag attgtaaatc gtttgggcga
    10621 catcttcaaa ataaggcgct ttttggacgc gttgattcgt gatccctgtt aaatgctgga
    10681 tttgttttga aatcttccgc cctggatgaa tatcgatcga aaaacgtgaa gtgatccgac
    10741 cagcttcaat catgacgcag ccaaattgga tgatccgatc ttcttttgga ttcgtcccag
    10801 tcgtctcgat atcaacgact gcataagttc ttggtttcaa aatatcttcc tcttctcctg
    10861 cctctaatgt tcttttgaaa ttatactaca aagtgcagaa aaatactctc atcgcttttt
    10921 aaggaaaaaa agacaaaaaa gcccagacac ataaatgtct ggacttttat ttttaatata
    10981 ggtaaattaa gctttagtaa cgtttgttgc ttgaggtccg cgttgacctt cttcaacgtc
    11041 gaaagttact gcttggcctt cttctaaagt tttgaatccg tcgccttgga tagctgagaa
    11101 atgaacgaat acatcgtttc cgttttctgc agtgatgaat ccaaatcctt tttctgagtt
    11161 aaaccatttt actgtacctg tttccataat aaaaatcctc cttgtgtatc aacacatatt
    11221 ttgcaattac ttgaatggtg tatatctgga agatgtttat ttgaaacaag ctaccacaga
    11281 ttaccgaaca aaaacta
//
LOCUS       DAXWLG010000080        11237 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXWLG010000080 DAXWLG010000000
VERSION     DAXWLG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.081"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            199..1458
                     /gene="lysA"
                     /locus_tag="ACGE4T_001685"
     CDS             199..1458
                     /gene="lysA"
                     /locus_tag="ACGE4T_001685"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFF8386960.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1445..2245
                     /gene="dapF"
                     /locus_tag="ACGE4T_001686"
     CDS             1445..2245
                     /gene="dapF"
                     /locus_tag="ACGE4T_001686"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFF8386961.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2248..3129
                     /gene="dapA"
                     /locus_tag="ACGE4T_001687"
     CDS             2248..3129
                     /gene="dapA"
                     /locus_tag="ACGE4T_001687"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFF8386962.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3126..3842
                     /gene="dapB"
                     /locus_tag="ACGE4T_001688"
     CDS             3126..3842
                     /gene="dapB"
                     /locus_tag="ACGE4T_001688"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFF8386963.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3848..4543
                     /gene="dapD"
                     /locus_tag="ACGE4T_001689"
     CDS             3848..4543
                     /gene="dapD"
                     /locus_tag="ACGE4T_001689"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFF8386964.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4559..5560
                     /locus_tag="ACGE4T_001690"
     CDS             4559..5560
                     /locus_tag="ACGE4T_001690"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFF8386965.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5557..6918
                     /locus_tag="ACGE4T_001691"
     CDS             5557..6918
                     /locus_tag="ACGE4T_001691"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFF8386966.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6878..8029
                     /locus_tag="ACGE4T_001692"
     CDS             6878..8029
                     /locus_tag="ACGE4T_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8386967.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8004..9173
                     /locus_tag="ACGE4T_001693"
     CDS             8004..9173
                     /locus_tag="ACGE4T_001693"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8386968.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9348..10727
                     /locus_tag="ACGE4T_001694"
     CDS             9348..10727
                     /locus_tag="ACGE4T_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8386969.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11008..11237
                     /locus_tag="ACGE4T_001695"
                     /pseudo
     CDS             11008..11237
                     /locus_tag="ACGE4T_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3741 a         1954 c         2504 g         3038 t
ORIGIN      
        1 gtttttatgt ataaatagct tctaatagtt cgggtatttt gtttttataa ccatttttat
       61 tcttattttt aaaaaaatat ttcgaaatat actagacaat tcagaaaatt ttgtttaata
      121 ttattccaat aaaattcaaa tctttatcaa atgaaattcc aataaaaacg gaaaaaaagt
      181 gaaaagagga atgaaaaaat gacagaattg ataattggcg gagtcacagc aagcacattg
      241 gcagaaaagt ataagacacc tctgtatgtg tatgatgaag aaaagatgag acaaacgatg
      301 ctgtcattca agcgaggctt tgtatcgaaa cgttttgata caaaagtgct ttacgcctct
      361 aaagcattcc aaacgattga gatgctgcat ttagttcgtg aatatggatt cgggctagat
      421 gttgtcagtg gtggggaaat ctataccgct ttgcaagctg attttcctag agatactatc
      481 tacttccatg gtaacaacaa gacgccagag gaactgatct atgcacttga aaatgatttg
      541 ttacatattg ttgccgataa tttgatggaa gttgaattat tagcagaatt aagccaacgc
      601 tatttaaaaa aaatttgtat catgcttcgg ttgaatgtcg gagtagaagc acatactcat
      661 gaatatatcg tcactgcgca tattgattca aaattcggca tgagctatga aagtaaagag
      721 tgccaacaag cccttcagtt gataaaagaa agcagctatc tagagttaga aggatttcat
      781 gctcatatcg ggtcacaaat ttttgatgtt actgcttggt tagctgaaat cgacaaattg
      841 acgaattact taaccgattt tgaccaacct ctttccttga atcttggcgg agggtttggg
      901 attcgttata cacaaaatga tcagccgatc ccagtcgaag aggcactaaa aaaactgatt
      961 gaatatacgg aagaagcttt ggaaaaacga aaactgacca ttcgtcaatt actgatcgaa
     1021 ccgggtagaa gtattgttgg agaagctggt acaacactat acacgattgg tttcatcaaa
     1081 aagacaccac ataaaaaata ttactttgta gatggcggga tgacagataa tatccgtcca
     1141 gctctgtatc aggcagaata cgattgtgat ttagcagcaa aactgtcgga agaaaaaaca
     1201 gagaaagtga cgattgcagg aaaaatgtgt gaatcaggcg atgtggtaat taaagaagct
     1261 tatttgccga aagcagaatc gggtgatctt ttagctgttt attccacggg agcttatgga
     1321 tactcgatga gcagcaacta caatcgagcg acacgaccag cagttgtttt tgtcaataat
     1381 ggccaatcaa gactgattgt tcgcagacaa gcattttctg atctactaag gggggaagtt
     1441 tcctatgatc cttcataaat ataatggctg cggaaatgat tttatcttat tagattacgc
     1501 agaagacaca gattatagtc aattagccgc ttctttatgc acaaaagaaa aatatgatat
     1561 agacggattg atcgccgtaa aaatcgatcc gttggaaatg atctattaca acaaagatgg
     1621 cagccgcgcg ccgatgtgcg gaaatggcat tcgctgtttt gctcgatacg taaatgatca
     1681 gaacatgatg aatgaacagg aattcaacgt agagacatta gcaggcacaa gaaaaattac
     1741 ggtgttggat ccctcacctt tttattgcag cgtagatatg ggagtgccag atgattctcc
     1801 ggaaaaaatc aaagctaatc aaacttatcc aataaaaaac caggaaatag agatcaatgg
     1861 tgaaaaagta acgatcacta gtttattttt aggaacgatt cataccgttg tttttgttga
     1921 aaatgcgcgt gaggaagtct ggagaaaaag aggtgaactg atctgtcatc atcctttgtt
     1981 caaagaaaaa accaatgtca actttgtaga agtagtcaat gaacaagaat tgatcgtccg
     2041 tacctatgaa agaggagtag gttggacact tgcttgcggt acaggttgct gtgcctctta
     2101 tgtggttgct aaggaaactg ggaaaatttc agcagatcag gtagttgttc acttggaaca
     2161 aggagacttg atgatctctg gtgaggatgc gatacaaatg gctggcccag cagttcatga
     2221 atggacaaga atattggagg aatgaagatg ataaaaggtt cgatcgtggc gctgatcacg
     2281 ccgatgaatg aagagggatc agtagattat gctggtttgg aaaaattgat ccaattccat
     2341 ttagatgaac aaacagatgg attattagtt ttaggtacaa caggagaatc ttctacgctc
     2401 acacaatctg aggaggagca gatccttcaa ctgacagtaa agaaagtggc tggccgtgtg
     2461 ccggtgattg caggagctgg aacgaataat acgaaagaaa cgattgaaaa agcgaagcat
     2521 tttgcttctt tgggggcaga cgcacttttg gtcatcaccc cttactacaa caaaacaagt
     2581 gatgcaggac tagctgctca ttttacagca attgcagaag cctcaccgat tccgcttatt
     2641 ttgtataatg ttccttctag aacaggcatg tcgattccaa tccatgtatt agtaaattta
     2701 gcagaacatc ctaatatcat tggtttgaaa gaagcctcag gagatatggc ttatgtgatg
     2761 gatgccgctc ggttgattgg agaagaattc ttcctttaca gtggaaatga tgatttgatc
     2821 ctaccagtaa tgagtgtagg aggaagcggt gtaataagtg tatgggcgaa tatccaacct
     2881 aaaatagtcc atgaattagt aaaagatacg caggacggaa gatggcagca agcaaaagaa
     2941 aagcaattaa atgcacttga actgatccat gccttgttca gtgagaccaa tcctattcct
     3001 gtcaaagcag ccatgtcatt attagactta ccttccggac ctctacgcct gcctttagtt
     3061 tctctttcaa aagagaaaaa gaaacaatta gctcagcttt tgttaaaaga aaggacggtt
     3121 gaaaaatgaa aatcggactg attggcagtg gacagatggg aaatagagtc gaagaagttg
     3181 cacaagaaag aggagatacg gtccttcttt tacggacggc acctaaagaa gcaactacgc
     3241 ttgcttttac ggaactgcca gaactgctta ttgatttttc tcatccagat aatttagaga
     3301 aaatcctttc gtatagctta tcatatcaac ttccgttagt cattggcacg acaggttata
     3361 cacaatcaca gttccaagaa ataaaagaac atgccaaaca tgtacctgtg atgtatagtg
     3421 ctaatttttc gttcggcatc atggtgatga atcaactaat caagcaagca gctgcaatgt
     3481 tgcaaggctg gcagatcgaa ctgattgaaa aacatcatag tcgtaaaaaa gatgcaccat
     3541 ctggtactgc aaatatgctg cttcaaacaa tccaagaagt gcaaaagctg cagcccgtat
     3601 acaactggca agggaaaaaa agagaagaaa accaaattgg tgtacacagt attcgtgcag
     3661 gttctttacc tggtgaacat gatgtattgt ttgctgctac tgatgaaata tttacgatca
     3721 aacatgaatc tttttctaat cgaatctttg ctgcaggcgc agtagaagca gcggactggt
     3781 tgaaagatca gtcaccaggt tattacaaat tagaagattt attaatggac aaaggggtgt
     3841 aaaaaacgtg gaagctcaag aaatcatccg ctacattcaa acggcatcta aaaagacgcc
     3901 tgtgaaagtc tatctgaatg taaccgagcc aatcgaattt aaagaaagta aagtttttgg
     3961 tgaaggaagc agcttcgttg tttttggtga ctatgagacg attgctccgg ttttagaagc
     4021 tgaatcagaa aaaatcattg aaatcgaaat cgaaactgct gcaagataca gcgcagttcc
     4081 tttgctagat attaaaaaaa ttaatgctcg tatcgaacca ggtgctatta tcagagatca
     4141 agtgtcaatc ggcaacaatg ccgtgatcat gatgggagcg attatcaata ttggtgcagt
     4201 aatcggtgaa aacacgatga ttgatatggg tgctgtctta ggtggacgag cgacagtggg
     4261 caaaaactgc catattggtg caggagctgt cttagcaggt gtgatcgaac cagcatcggc
     4321 caaaccggtt atcgtagaag acggcgtact ggtaggtgcg aatgcagtga ttgttgaagg
     4381 tgtccatatc ggaaaagatg cagtcgtggc agcaggagca gtggttttag aagatgtggc
     4441 agcagaaact gttgtcggtg gaatacctgc acgtgtatta aaaataagtg atgatcagac
     4501 aaaagaaaat acagcattga ttgctgcgtt acgcgaatta tagaggagat ggagaaaaat
     4561 gcgcgaaata gatacctgta ttgtgggggc aaccggatta gtcggccaaa cgatgctcaa
     4621 agtgttagaa gaatatgatt ttccagtagg acgattgaaa ttattagctt caaaacgttc
     4681 agccggtaaa gtttgtgtat tcaagaaaaa gaaacacact gtacaggaat tgacagaaac
     4741 ttcatttgaa ggatatgatt tagcgctatt ttctgcaggt gcttctgtat ccaaagagtt
     4801 tgcgccaatc gcacgaagtc agggactatt tgtcatagat aattcctcag catggcggga
     4861 agattcggat attccattaa tcgttccaga ggtcaatctg gaagactata cattgaatcg
     4921 gctgattgca aatccaaatt gttcaaccat tcaagcggta cttccgctta aagctttaca
     4981 agaagccttt ggtatccagc gagtcaatta ttcgacctat caagcagttt ctggttccgg
     5041 gcaaaaaggc attgaagatc ttcatcgtac gcaaaatgac gaggaaccag ttttgtatcc
     5101 ttataatatt agccaaacgg tgattccgga aatcgatcag cctttggaaa atgggtatac
     5161 aaaagaagaa cagaaaatga taaatgagac ccgtaagatt ctgcatcagc ctgctttgcc
     5221 agtatcagca acctgcgtac gtgtgccaat cgaaaatgga catggagtct ctgtggcagt
     5281 tcaactgcaa aaagagttta cgacggaaga aattagagag tgtctcagcc agtttccagg
     5341 gattcaatta gaagatgatc tagcagcaca taaatatccg acgagcattc ttgctagagg
     5401 aacagatatg gtttatgttg ggaggatcag aaaagacact tctttagaga acggattgtt
     5461 gttctatacg actgccgata acatacgcaa aggagccgct gcgaatgccg tacagattgc
     5521 tgcagcttta atgcagaaaa tagcggaggt ggcaagatga gtaatacgct gaaagtagcg
     5581 aaattcggcg gaacatcgat gtctcatgaa gagcaattca aaaaagtccg tacaattgtt
     5641 ctttcagatc ccaatcgcca agtgatcgtt gtttctgctt taggcaaacg tacgtcagat
     5701 gatgacaaag taacagactt actttatttg atttatgctt accttcagca tcatgttgat
     5761 tggacacccc tttggaaaag gatttgtgat cgtttcattc aagtaagaga tgttcttgcc
     5821 ctgcagttac ctattgaaga tgaactagca cgcatacaga aacaactgga agacgaaaag
     5881 ataactgaag attatttagt aagtagaggt gaatatctga ctgctcaact gatggctgag
     5941 tatcttggtt accagtttat tgacgcaaaa gacctgattt tctttgacta tgatgggaaa
     6001 atcgatcagg aaaaaacagc acaggaaatc acaaaagcat ttaaaagata tccgaaaatg
     6061 gttgttccag ggttttacgg ggcgaaccct gcaggaaaag aaaaattatt gggccgtggg
     6121 ggaagcgaca ttaccggcgc gatactggcg aagatgttgc aagcggaaag ttatgagaat
     6181 tggacggatg tttcaggaat catgatggct gatccgcgaa tcatcgatca tcctaaaatc
     6241 atcgaggaaa tctcttttag agaactgaga gagatggctt atatgggggc taacgttctt
     6301 catgaagcag ctgttttccc agttcaagag gctgatatcc ccatccaaat aaaaaatacc
     6361 aatgatcctt tcgctcaagg gacaaaaata tcgaatcacg aaatagaaaa ggaaaatgga
     6421 ctgacaggaa tagctggacg caaggatttt ctttccatca ctttattcaa acgtcatatg
     6481 tccgatgaaa tcgggtttat ctggaaagca atgagcattt ttgctaaaca tgagatatca
     6541 atcgaacata ttccttcagg gatcgacaat atcggagtga tcgtctcagc agaagcgatt
     6601 gccgataagt tatttctcat cacgaaagaa ttaaaagaaa cacttggcgt ggaagaaatc
     6661 gtcgtgatcg aagacttagc tttgatctct gtcgttgggg gtccgcataa agaattgatt
     6721 ggattatctg ggaaagtgtt atcgatattg aacaaattag aaattcgcac atcgatctta
     6781 tcacaaggag cacaggagtt gaatctgatc atcggtgtac cgaataatca atatgaaaca
     6841 gtagtgaagg gtatctatga agggatggtg aatacggatg ctgaaagaat atcgaatggc
     6901 actccatcaa attcctgaaa tcgggtttca tgaattcaag acaaaaaaat atttatatga
     6961 acaagtaaaa aacagcgagg ccgttatcca tgaaatcggc gaaacaggac tactccttta
     7021 ttttgataat cagcaagaag aaacgattgc ttttcgaaca gatatcgatg cactgccagt
     7081 caaagaagaa acgaatctct cttttgcttc taagcatgaa ggttatatgc atgcatgtgg
     7141 tcatgacgga catatggcga tgcttttagg gttgactgac tttataacta aacacattga
     7201 ggatcttatt tataatgtag tactgatcta tcagccatca gaagaaattt ccggtggcgc
     7261 acaaagcgtt attcagtcag gattgttgga taaatatgaa gtacaagcaa tatttggctt
     7321 tcatctatgg cctggactag ctaagggaga agttttctct cgaccaggac ctttgatggc
     7381 acaaagcagt gaaacagaca ttatcattaa aggccagtct gcacatattg cttctagtga
     7441 aaaaggaatc gatagtctgg aggcagctgt acgatttttg aagaatgttt atgattttga
     7501 ccggcaatta ccggcagagt ggaatcatct aatgaaattt ggccaaatcc aaggtggaac
     7561 gatccgaaat gttttagctg gagaagttat tgtatccgga agtatccgtt cttacagttc
     7621 agatatgcaa aattatttga agaggggatt agaccattta gcagaacagt ttcactctac
     7681 agctactatt tcttttcgct ataatgacgg ctatccagct gtaatcaacc atagaggctt
     7741 atatgcagca ctgttagatt caaacaaact acatgaatta gatgctccag ttcttcaagc
     7801 cgaagatttt ggcatatata cgcaacaata tccatgtgta tttttctttt taggtgtcgg
     7861 tgatacccct gctttacatg atccgacgtt cgattttgat atggatgttt tagaaaatgg
     7921 tgtagaatgg tacaaaacga ttctttttac agaaaatctt cttgtaaaaa tcgatagcag
     7981 ttcaaaagag gagcgagtaa ataatgatga atttatccaa tcgatttagt cctaggcttt
     8041 ctagaataga agtatcaaaa atacgacaat tcgatcagca aatatcgtcg ataccagatg
     8101 tgatcaaact caccctggga gaaccggatt ttccaactcc tgaacatgtc aagcaagcag
     8161 gaatagcagc gattgaagaa gacttttctc actataccgg aatgagagga ttagaggaac
     8221 tgcgggaagc ggcttgtata tttcagcaac agcgttatgg attaacttat gatccgcaaa
     8281 cggaagtttt gacgacagtt ggtgcaacgg aagcgattgc ctctgcgttg ttatctgttt
     8341 tggaagaagg agacaaggtt cttattccag cgcctgctta ttcaggatat cagccattag
     8401 tggaactagc tggtgctgaa ttgatcccga tagatacttc tgacacaggg tttgtctgtc
     8461 agccggaaca atttgaacga gcatttgaac aatatggatc agctgtcaaa gcagtcattt
     8521 taaactatcc taacaatcct actgggacga cattgtctgc aaaacaattg aaagcaatcg
     8581 ctgaggtttt aaaaaaatat ccggtatttg ttataagtga tgaagtatat gctgaattga
     8641 cttatagtgg aacgcatatg tccatagcta cttatcttcc agaacaaaca atcgtgataa
     8701 gcgggttatc caagtcacat gcgatgactg gttggcgagt gggctttatt tttgctcaaa
     8761 aagcccttat tgacgaacta attaaagtcc atcaatatct ggtgactgcc gctacaacga
     8821 tgtcgcaaaa agctgcggct gaagcgctaa taaatggggt gaaagatagc gagaatatga
     8881 aagagcaata tcaaacacga agagactatc tggtccagca attggcgcct ttaggatttg
     8941 aagtgaccca gccaaacggt gcattttact tgttttgcaa actcccagct gccattcaaa
     9001 ctgactcttg gcagttttgt gtagaattag cggaacaggc aaaagttgcg tgtatcccag
     9061 gaattgcatt tggcccagaa ggagaaggtt atatccggat ttcttatgct agcagcatgg
     9121 aaaaattaca tgaggcctgc aaacggatca aagacttttt aacagaacag tgattcatag
     9181 attttatcaa aaaaacaatc gttaattttg cggtatattc tgcaaggaga cgattgtttt
     9241 ttaaatttag ttcaaatgaa cgcgtttctt ttgattcata actaatgtaa tgttaagttt
     9301 aaattgttta gaatgatttt aaaagaatat tcaaggaggt ttcatcaatg gaaaaaatca
     9361 aagttgtcat tgtaggtgca tctcatggag ggcatcaatc gatcttggaa ctattgacac
     9421 gatacggtga aaatgtagat atcacgctat ttgaagcagg agactatatt tccttcatgt
     9481 catgtggtat ggagctgtat ttagaagaca aggtaacaga tgtaaatgat gtacgtaatt
     9541 tcagaccaga aaactttccg caatccaatg ttcatatttt aaataatcac gaagtaaaaa
     9601 cgatcgatgc cgataaaaaa acagtaacgg ttactagcgc atcagataac gaaacaaaag
     9661 aatatcctta tgacaaattg attttgagtt ctggtgtcaa accaaacaat ctgccagtac
     9721 caggagcaga tctagaaaat gtctacttga tgcgtggata caattgggca accaaaatca
     9781 aagaaaaatt aaatgatccg gctattaaaa aaatcgctgt cgtaggtgcg ggttacattg
     9841 ggatcgaagc agcagaagca agccgaaaag ctgggaaaga agtcgtattg cttgatgtga
     9901 tcgatagacc tttaggcact tatttagatg ctgagatgac agatatttta gaacaacacc
     9961 taaatgaaaa cggtattgaa gttttgacaa atgcaaaaat cgaagctttt actggtaacg
    10021 gaaaagttga ggcaatcaaa acaagtgaaa aagaaatccc agcagatcta gtcattcaag
    10081 ctgccggtgt gaaggcaaat acagaatggt taaaagggat cgtcgactta gatgaacgtg
    10141 gctggatcca aacaaatgaa tacttgcaaa cgaatcttcc agatgtgtat gcagtaggag
    10201 atgctacact tgcttattct atcccagcac gcaaaaaaat gccgatcgct ttagcgactg
    10261 ttgcacgtag agaagcacgt tatgtagtaa aacatctttt tgaagaagta ccaaacaaac
    10321 cgttcggcgg agtcgtaggt tcctctgcat taagtgtctt tgactatcat tttgcagcaa
    10381 gcggattgaa tagctttaca gcagcaaaag caggagtaga tgtacgagtg tcttattatg
    10441 aagatacgtt acgaccaaaa tatgtaccag aagaacaagg aaatccaaaa gtgtccatcc
    10501 aattattctc ggatccaatg acccatcaat tgctaggagg agcagtatta tctacctatg
    10561 atgttacagg tcaaggaaat gtattggcac tagcgatcca aaacaaactg actttagaag
    10621 aactagcaga agctgacttc ttcttccagc caggctttga ccgacaatgg agcatgttga
    10681 atctagccgc tcaacaagcg ttaggagaag aaccatttgt agaataagaa aacgaaatat
    10741 ttgaaaagag gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa
    10801 cagcttctta tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac
    10861 cgtttatttt caaaaaaggg actgtgacac gatttgtgta ccgaccccca aaagttagac
    10921 caaaaaatct aacttttgga ggtcggtatt ttttatggtt aaatatactt ttgaacttaa
    10981 attaaagatc gttcatgatt atttggatgg caaaggcgat tggaccgtaa aaatccagat
    11041 caagagattg aagcggaaat gcttaaaatt cgggagaagc acaaggatta tggctgttta
    11101 cggatgacca atgaactaag aaaccgcggt ttctctatca ataaaaagaa agttcagcgc
    11161 ttgatcaaga agctaggaat aaaagtaacg gcatacacgc gtaaatcacg tcgctacaat
    11221 tcttatcgag gaaaagt
//
LOCUS       DAXWLG010000081        11195 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXWLG010000081 DAXWLG010000000
VERSION     DAXWLG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.082"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            30..1112
                     /locus_tag="ACGE4T_001696"
     CDS             30..1112
                     /locus_tag="ACGE4T_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8386970.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            1446..2384
                     /gene="yidC"
                     /locus_tag="ACGE4T_001697"
     CDS             1446..2384
                     /gene="yidC"
                     /locus_tag="ACGE4T_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFF8386971.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(2565..2969)
                     /locus_tag="ACGE4T_001698"
     CDS             complement(2565..2969)
                     /locus_tag="ACGE4T_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8386972.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(2985..3794)
                     /locus_tag="ACGE4T_001699"
     CDS             complement(2985..3794)
                     /locus_tag="ACGE4T_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8386973.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(3781..4671)
                     /locus_tag="ACGE4T_001700"
     CDS             complement(3781..4671)
                     /locus_tag="ACGE4T_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8386974.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(4684..5163)
                     /locus_tag="ACGE4T_001701"
     CDS             complement(4684..5163)
                     /locus_tag="ACGE4T_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8386975.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(5180..6946)
                     /locus_tag="ACGE4T_001702"
     CDS             complement(5180..6946)
                     /locus_tag="ACGE4T_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFF8386976.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFATGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(6992..7933)
                     /locus_tag="ACGE4T_001703"
     CDS             complement(6992..7933)
                     /locus_tag="ACGE4T_001703"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFF8386977.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(7930..8925)
                     /gene="lacD"
                     /locus_tag="ACGE4T_001704"
     CDS             complement(7930..8925)
                     /gene="lacD"
                     /locus_tag="ACGE4T_001704"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8386978.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(8918..10111)
                     /locus_tag="ACGE4T_001705"
     CDS             complement(8918..10111)
                     /locus_tag="ACGE4T_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8386979.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(10129..10857)
                     /locus_tag="ACGE4T_001706"
     CDS             complement(10129..10857)
                     /locus_tag="ACGE4T_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8386980.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         3216 a         2430 c         1982 g         3567 t
ORIGIN      
        1 ttaaaaaaat aatggaagaa gttttttata tgacaaaaaa tttaattgtt cgcggtgccc
       61 ctcaagagta tgaaataaaa gaaaacgcat gggattcact ccctgaacac ctttacaaaa
      121 ggcatattga caacgttttg attctccatg gaaaaacatc ttggaaagca gtaaaagatg
      181 tttttccaga tttatcaata acaaaaattt ttgaatatta tggtggagaa tgtaccgatc
      241 cacttgctga cagttttgtt tctttatgca aaaagaagga tttcaatggt attattgcag
      301 tcggcggcgg gaaagtagca gatctaggaa aattgattgc ctataagaca aacttaccaa
      361 tcatcatctt acctacctta gctgcgacgt gcgcagctta tactccactt agtgtggtct
      421 ataatgaaga aggagtgatg cttcgctatg atatgttccc taaagcaaat gatttagtgt
      481 tgatcgatcc aaagatcatt ttgcactccc ctcgcgagat gatgattgcc ggtattggtg
      541 acaccttagc aaaatggtat gaatctcacg caatcatctc tcagttaact atcaagccta
      601 tcgaagtcca agtagcggaa tttgcagcgt tacgatgccg cgaaatcctt ttagagaaaa
      661 gtgcagaggc aatggaagcg atggaccagc aaaaaatcaa tcaagctttt atcgatgtcg
      721 tggaaacaaa cattttattg gctggtatgg ttggtgggtt tggcgatgac tacggccgaa
      781 cagctggagc tcattccatt cacgatgcga tgaccgtact ccctgatagc catcagcaac
      841 ttcatggaaa caaagttgca tacggtatcc ttgtccagct gatgatcgaa aataaattag
      901 cagaaatcga acacctactg ccgttttatc atcacttgaa cttaccaacg agtttagctg
      961 atatgggttt acatttaaca gaaagtgact atcaagccgt agctgaacgt gcatgtttac
     1021 ctgatgaaat gatccacttc atgaaagaaa cgatcacacc agcactggtt gtccagaaaa
     1081 tgaaagagtt agaacaagtg acacagggat agttagcccc ttataccatg gcagtcagct
     1141 tttttatgga tcggctcaat ggtaaaaaaa aagaagtgga aaaaattgac attccacttc
     1201 ttttttttgc gctaaaatta tgatttcaag gaaatatccg ttgctcatct tattttagag
     1261 aacggaggtg ctaagtatgc tttatactga agtattttta gctggattct tctttttaac
     1321 attaagcgtc ttttttgatc ctccaatcgg ttatttctat gaaatttcta tgaagttgat
     1381 tgaattatga gcgctttttt agtatagtta agcttgtgta aaaattataa aagaggaagt
     1441 attttatgaa taaatggaaa aattggttat tgggctctgg gcttgtcacg atcatgcttt
     1501 tcttgtccgg ttgtgtccgc atggattcaa atggtaaccc tgacacttca ggaatcattt
     1561 accgtatatt ggttcaccca atgggacaag cgattacata tttagtggaa aactttaatt
     1621 ggtcctacgg ctgggctgtt atcttgatga cagtcatcgt tcgtatcatc attttgccat
     1681 taggtattag tcaatcgaaa aaaacaatga tccaatctga aaagatgcaa gcattgaaac
     1741 cacaagttga agctacgcaa caaaaattga aagctgcgac aactcgagaa gaacaaatgg
     1801 ctgcacaagc tgaaatgcaa caagtttatc gtgaaaatgg cttaagtatg actggtggta
     1861 tcggttgttt accgcttctg atccaaatgc cgatcttttc tgcgctatat ttcactgctc
     1921 gctatacaga aggcatccgt gaatcaacct tttacgggat cgacctaggt aaccctagct
     1981 tagttttagt agcgattgcc ggtattgctt atttacttca aggctatatc tcaactatcg
     2041 gtatccctga agaacaaaag aaaacgatgc gtacgatgtt gatcgtatca cccgctatga
     2101 tcgtcttcat gtcgatcagt gccccagctg gtgtcactct ttactgggta gtcggtggga
     2161 tctttagttg tctacaaaca ttcattacga atgtcatcat gaaaccaaga ttaaaagcgc
     2221 aagttgcgga agaattaaaa caaaatccgc caaaacaagt ggtgacgcct cgtaaagatg
     2281 tcacacctgc tgaacctgcg aagcaaacaa aaaaacaagt acaagcacca aaaaacaatg
     2341 cgaagaatgg tcggaatgct ggtaaacagc aaaagcgtaa atagttattt gtaactagct
     2401 tcttcgatcg acccagatca tttctgattt tatgacgata aaagccaacg tactgtgaaa
     2461 tatagaaaac tatatttcgc ggtacgttgg cttttttata cacggcctct ttatacatcc
     2521 gtatttatat ccagttgtat caccagtcaa gaagttgtgc ttgattattc ttcttcatcg
     2581 tctcccatgc cagcaatcac ttggttcact gaaacgatac tctcacgacc cgaaccaata
     2641 aaatctggtt ctgctccaat catttgcgaa ttgatgaatt cgatgaccat cggcaaattc
     2701 atgccagcat atagttcgat cgtttcacct tccatgatta gcttggaaac ggtatttgca
     2761 ggtgtcccac ccaacagatc gcaaagcact acgtaatctt tcaatggtgc tgtaacggag
     2821 aggaatcttt ctttatacgt gtccgcgcca tcttcaggca ataaagggat cgtatagata
     2881 ttttcttgtg gtcccatgat catttctgta ctgtttttta gttcttcaca aaatttacca
     2941 tgactaatca agactagttg tctactcatc tttccaattc tcccttattt cgaaatcaca
     3001 cctaatgcag ataatgctac acatacgatc aatacgatga atattgctcg agtaggtgtc
     3061 atatttttct tacctaacat ccaataaacg ccaccaacga ttccagctgg taaaagttta
     3121 ggtaagatca tatcgatata gttttgcata ttcaacgtta catccccaat cgttggtgcc
     3181 catgagaatt taacattgat catggttgct actaacgctc caaccatgaa aactcctagt
     3241 aaagtagccg catctgtcaa tgcagtcaag cgtgattgca tcgtggtaac tagagaaatc
     3301 ccttctttgt aggcaaattc tagttgtttc caacgaaaga ccatgacagc gacctgagca
     3361 aggatccaga taaggatacc tactggatta ccatctgttg ccatatttgc ggctaaagct
     3421 ccaaatatgg tcgggatcaa tgccgcaaat acagaatcac caattgcagc gaaggaaccc
     3481 atcaatcctg ctttcatccc agaaacagtt tcctttgaag agactccttc tttttcttcg
     3541 atagcaagat cgatcccagt aatgatcgtg ttaaagaaat tagacgtatt aaaaaattgc
     3601 gtatgtgttt tcatcatttc ttttaattct ggtgtatcat caccatagat ttttctaagt
     3661 tgtggcaaga tagtatacag ataacctgaa ccttgcattc tttcatagtt ccaacctaat
     3721 tggaaagtaa acaaacttct acgattgatc tgcttaaaat cttctttggt caatttataa
     3781 ttagatttcg tcatcttcga tttccccctc atcagattgt gattgctgac ttactactgg
     3841 atgttggtca ttcttttgat cattcttata actgatcgtt gccagtgcta gaccaatcaa
     3901 tgccacagca agcatcggta atgaattgaa tgttcctggg aaatctttga ccacgctagc
     3961 cacacttgtt cctaacaatt ggacattcga gaacactgtt gctaataacg ctgtgatcgt
     4021 aaatcctaaa atcagataag aaatgtgttt ctttactggt aggtagcgta gtaaaattgc
     4081 aaacccaact gctggtaaga ctgcccctgc aacagataag ccatctccta gccattttag
     4141 gtcgccgttt aaaacagcca caacattttg gactaagcca ccaccgaatg ctagggccaa
     4201 gaatacagga accattctgg aaagtgccca aggtaatgcc cccatcaaga agttccgttc
     4261 aattgctttg taattcattg tttccacatg ttgatcgatc cgatgtgcaa aatacgtatt
     4321 cgcaaaacgt gccaaaatat ctaattggat catcaaactt gctacaggaa ccgcgatggc
     4381 cgcaatcgct tgttctgggt tcatccctag agaaatggag aaagctgttg ctaagatcgt
     4441 tccagagttg gcatcgattt ttgatgcacc accaaatgtc cctaccccta aaacagtcaa
     4501 ctgcatactt ccaccaatga ttaaacctgc ttttaaatca cccattacta acccagcata
     4561 taatcctgca aaaactgggg cgctcattga agaataaatc tgtaattcat ctaaaatttg
     4621 ataccctgca tataatgtta ataataatat ctgccaccat tgaatatcca tgaatcattc
     4681 ctcctatttg attaaaagac tcataaagtc ttctgctttg tcatttggca ccatttgagc
     4741 gatcaaatga acgccaagct gatctaattt cttgaagacc tctacatccg catctacgac
     4801 attgattgat tttgtgatgg aacgtgtttc atctgtttgt gacatattgc cgacatttaa
     4861 ttctttgatc ggtacacctt tttctactag cgctaagata cgatctggtt ttttcgctac
     4921 gatcagcact cgttgagaat catatttacc tgctaaaata tttgcggctg ctttttcaat
     4981 aggtaaaata cttaatttga ctccagctgg cgttgccaac tttaacccac ttttttcgat
     5041 cgcatttgtt gctacctcat cgtctacgac catgatccga ccaatattca actttgtcgt
     5101 ccaaagattt gccacttgtc catgtatcaa acgtccatca attcttactc caataattgc
     5161 catcatccat tctccttttc taactttttt ctattggttc tttgacaaaa tctataactt
     5221 cactgaatct accttctgtt tcaaagataa cgatttcatt ttgtcctttt ctaaataaac
     5281 cctcgggaac aaacagtgaa agaattggtc ctacttccca aaaacgacca atattcgtgc
     5341 cattcaccca aacgatccct ttcccaaact cactaaggtc caaataagta tcaccgattt
     5401 ctgacagttc cgcttcaaag cggtaaaatg ctggttgacc tgcttcccat ttttttgaaa
     5461 aatcgatgtg ttctgtagtc tctaaaggta aacaatactg tgtccattct gtgataaaat
     5521 gaagatcgct catcactcct gtacgtattc cttttctttg cgtatcagct agcagcttgt
     5581 gtccatagtt gacccgtccc atattttcaa ataatagatc aatttgatgg tcttctttct
     5641 caagtgtcaa tggaatggtc tcaccgatct ctgtttggta ttgaatcgct tgcttttctt
     5701 catcgagaaa aacatggacc cgatcacgtc cgtcgatgat ccgtaatttt tcattgaccg
     5761 catctttttt gatcgttgtt cgatacaata ggtacccagt attctgaccc aattcttcca
     5821 ttgtttgagg ataggtcgag tggatcggct tgctgatggt atccagtgtt gcgaaaaagc
     5881 tgactttttc tttcaagttg attccttttt tgacgattgt ttcttttatc catggttcca
     5941 tttgctgcat ttctggaaaa ttttctgcca tcattcgttt cagagcaaaa tatttttccg
     6001 ttggatttcc ctgctcatcc aatggtgcat cgtaatcata ggaagtgact tgcggcaaat
     6061 caatcgcgcc tctggctgaa cagccattca taaacccaaa attggttccg ccatggaaca
     6121 tatagagatt gatactgccg atctgtaatg cttctttgac tgcatccgct aattcttccg
     6181 gatctctttt aatgatcggc tctttccaac gattaaacca gccatcccaa aattccatac
     6241 acatcaacgg ccatttttta ccatgtgctt caaaaaactg ggccatatct gcaaaatttt
     6301 cttttgcttt tgagccaaaa ttccctgtcg caaaaacatc atcgtcaatc aaaatgcctg
     6361 ctcttaatgt ggcttgccaa ggtccatcag aagtgaatag cggtacatcg attccacctt
     6421 cctccaagta tttttttact tggcgcaggt atgctttctc ttcaccatac gaaccgtact
     6481 cattttctac ctgcaccatt aagacgttgc caccatgagt gactagatga ggaacgattt
     6541 taggtagtag cgtttggtaa taccgtttga catacttcat gaagcgagga tccgctgaac
     6601 gaatacggca ctcctttgtc aatagccaag caggcaagcc accaaattcc cattctgcac
     6661 aaatataagg ggctggacgt acgattgcgt ataatccaag ggattgcgct aactctagaa
     6721 aatgggaaag atccaatatt ttcgaaaaat cataggttcc ttcttctttt tcatggagat
     6781 tccaaggcac ataggtttcc acagtattaa atccaagcgc tttcaaattg tgcaatgacc
     6841 gctcccaatt cgtcggatgc actcggaaat aatggatggc accagataaa attttgaaag
     6901 gttgaccgtc taacaaaaac tcttctttta tttcaaatct agtcaagcaa atttcctcct
     6961 cgtttttaga aaccgctttc taattttata atcaacgaat ctttctgacg ttgatttccc
     7021 ccctaaaata atcgactttc acaggatcga tccatcctgt tttttcttgt tgtgcattcg
     7081 ccatcccaca ggccatcgca aacgcaagtg tttcttcaat cgtatagctc tttgatagag
     7141 catgtgctaa gcctgcaata gtcgaatctc ctgaccccac aggattcacc accgcgattt
     7201 taggaatcgt aacctgatag aagtgtctcc ctactttagc gattgctcct tttgcgccta
     7261 gcgaaagaac gatccaattg attccttcaa aaagcggatg atccaataat gattggattt
     7321 catctaacga agtagccgaa gaaattgttt ggtggaccaa ctctcctaat tcttccaaat
     7381 taggtttgat caaaaatggt ttttccttgc cttccaagac ttcaagcaag gtttgaccag
     7441 aagtatctaa caatactcgt gttccttttt tattcgttag ggcaagtaac tctgcataat
     7501 aacttttaga aaatccttga ggcagactac ccgaaatcgt aaagatatca gttgtttcca
     7561 ttagtcgtgc aaaatgctcg gtaaatttat gttgctcttc ttccgtcaca acaggaccgc
     7621 tttccaatag ttctgtttga ttcactccat gtaaaatagc aatcgaatct ctcgtttccc
     7681 ctttgatagg taaaaattga tgcgttatcc ctgattgatc gagttgttct ttgatatatg
     7741 ctccatgctg cccgccgact aatccagtag ctaaaacatc atctgctaat aaatgaagga
     7801 cacgagcaac attcaagcct tttcctccag ccgttttgtt gaccgttttg acccgattga
     7861 cttggtcgat ccgtaattca tccaacgtgt aagcgatgtc aattgaagga ttcatggtaa
     7921 ctgtcgtgat cattttgctg tcaccgaatc ttcttttact ttgtcaaacc aaggggtggc
     7981 gttttcattt atcactttat tcaacgattc gatattttgt cgcccttgtg ttctcaacca
     8041 ctcaatcgca ccttctctac cattttcaac atatgctttg actccatctt tccaagtcgc
     8101 acgtccgcat aacacaccgt tgaatgttga accagattca tgagcaaaga tcaatgtttg
     8161 ttggaacaat gcagtcgaaa cccctgcact caagaagata aatggtaacg gtgttgcatc
     8221 actttgttcc ttgaagaatg ccgctgcttc ttttttacta tacgcaattt caccatcaga
     8281 ataaccttct acatagttca tattgactgg cacttctact ttcaatacat ccactttata
     8341 tttaggatca gaaaattctt tcatcatctc gatcacttta tgcggtttga tcttcgcata
     8401 ttctaatgaa gtgctgtcat tggttttggc atcatacgaa accaattcta gataaaacgg
     8461 acgcttttca cctgcacatt cactgcctaa acgttcgata aatacatgtt tcaaatgatt
     8521 gatctctggg tcttcatcta catcataata aagcaaaaac ttgatcgcat cggctgtttg
     8581 ttcctttaat ctaagcgtag accaatccgc taataaatca ggcagacgtc ctggagttgt
     8641 cgcatcataa cccgtttttt cataggctaa aagtagtcct gcttcctgat gtctggcttt
     8701 agcagctggc aatccatact ctggatctag taaaatagca gaagcataag gcgttaattc
     8761 tgctgatact aattctttga attcagtgat ctgttgcgcc gttggttctt ttcctaacgc
     8821 tttgatcatc tttttcaaag caccgcgttg atcaattgcc aatgcactga tgattccttc
     8881 ttttgttgat aaactcttca tagctgcaat tttttcttca ttcatccttc gtttcccctt
     8941 tgttcacata ttcatggata atgacacctt tgaccactcg attgactgta cctgtaggcg
     9001 atggtgtatc tggtttattc gctactttta tcgacgtatc gagcgcaatg atttgggctg
     9061 tcatgatcat aggtaaggca agatacgcat caggtaggct gatcgaagct tctaacaaaa
     9121 gacttgtacc tgtgaaatcg atgtttcctg gttgggcgat cgcgatgaca ctcgtggtaa
     9181 tttggtcatc ttttatttct tctagaatat ctaagtcata atttcttgta tacggttggt
     9241 tattaacaaa accaataaca agtgtttcgc tatttaaaaa cgattttggt ccatggcgga
     9301 agcccataga agaatcaaag atcgttgcca ctttaccggc tgtcaattcc aatattttca
     9361 attgtgcttc tctcgttgca ccagccaaac ttcctgaacc aagataggcg atacgttcaa
     9421 atgacttagg caataatttt tggatttctt gatctcgtga aattagatct tctccagttc
     9481 tgatcaattg ttgaagataa ctttcttttt tctcaacggc agtttggtcg aatacccata
     9541 acgcactcaa caacatgcac gtaaaacttc ctgtcatggc aaatcctgca tcatttgatt
     9601 tttctggcat cagtaaaaga taatttcgtt catctgtcgc tgcattcttt gctaactgac
     9661 cttctttagc acaagtaatc gtcaaatgaa acaatgtctt gatttgttca ttggcaatct
     9721 tgactgttgc cacactttct ggactatttc cactacgtgc aaatgacacc agtaatgtcg
     9781 gttcatccgg aaatagaaat tcttttggtg aagacacgat atctgtcgta ccaaagctat
     9841 gaaaaataaa gtgatcgtta tcaccgtatt catttaaata aggtactgct gtatccccga
     9901 catattgtga tgtgcctgca ccagtaaaaa tcactcgaac tcttttgcca tttgctgctt
     9961 tttttatctg gtcaaaaaac aatgctaatt tttcttgatt ctgtgctaat atctcagctg
    10021 tttctttcca taattgcggt tgctgtttga tttctcgcgt agttatttcc gcacccaact
    10081 cttttaattt atccttcgat agttcaaaca tgttgttttc cctctcaatc attttcggac
    10141 gtggcggatc ttataatgaa actggtctgc tctcgctaca ctatccgtat attcaataat
    10201 ttcatttttc acattatagg tcgttcggct tagatataat accggggcac catcagcgat
    10261 acgaagcgga tcagcatctt tcttttgaac gatacttgca taaaattctt catctgctaa
    10321 ttggacttgc tgttcataat cctgagaaaa cacatcatac aatggcttac tttcaagtaa
    10381 tggcagatca agaccaataa atagttttgc aggtaaataa gaacgttcca ccatcaatgg
    10441 ttcgtcatcc gctaaacgaa gtcttttcaa tttgaaaact gtttcgccta aattgatgtt
    10501 tagattcttg gcaacctttt tgtttgcttc catttgcttg aattccaaga tgactgtatt
    10561 aggcacccgt cccaattccc tcatctcttc tgtaaaacta tacgctcccg ccagatcaga
    10621 cgacttatct gatagatcag acacatatgt tccttttccg tgtcggcgat aaatataccc
    10681 ttgcgtctct aattcttgca aagcaaggcg aaccgttgtc cgactcactc cataatgctg
    10741 acaaagttct ctttcagata acaatttgtc atgaggtgtc atctgtgttt cgatacgttc
    10801 tttcaatacg tcaactagtt gataatataa tgattttcct ttacttgagc tctccataaa
    10861 aatacctctt ctttactggt tataaccaca acacaagaat acccacatat tcctcatttg
    10921 tcaaagggtt tttggcgtaa aaaagctatt ataacaaggt ttcaaggtaa tttagaactg
    10981 gtatcaaaag atacctcaaa aataccagtt gacaaataaa aaacaaaaaa aagctgaatc
    11041 ttggcaaaga ttcagctaaa tggtctagag aagtaaagaa aaaatattct ttatacatct
    11101 gtggctattt ctactatcga caagcaaatg ggcctaatag actactatgt aaataaaaat
    11161 ggctttctct aaataaagta ggatttttta ttttt
//
LOCUS       DAXWLG010000082        11187 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXWLG010000082 DAXWLG010000000
VERSION     DAXWLG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.083"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            6..305
                     /locus_tag="ACGE4T_001707"
     CDS             6..305
                     /locus_tag="ACGE4T_001707"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8386981.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACGE4T_001708"
     CDS             378..1739
                     /locus_tag="ACGE4T_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8386982.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACGE4T_001709"
     CDS             1761..2021
                     /locus_tag="ACGE4T_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386983.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACGE4T_001710"
     CDS             2035..2787
                     /locus_tag="ACGE4T_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFF8386984.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACGE4T_001711"
     CDS             complement(2881..3486)
                     /locus_tag="ACGE4T_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8386985.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACGE4T_001712"
     CDS             complement(3539..4513)
                     /locus_tag="ACGE4T_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFF8386986.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACGE4T_001713"
     CDS             complement(4891..5610)
                     /locus_tag="ACGE4T_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8386987.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACGE4T_001714"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACGE4T_001714"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFF8386988.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACGE4T_001715"
     CDS             7161..8558
                     /locus_tag="ACGE4T_001715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8386989.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACGE4T_001716"
     CDS             8639..9079
                     /locus_tag="ACGE4T_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8386990.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACGE4T_001717"
     CDS             9227..9553
                     /locus_tag="ACGE4T_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8386991.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACGE4T_001718"
     CDS             9554..10303
                     /locus_tag="ACGE4T_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFF8386992.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACGE4T_001719"
     CDS             complement(10383..10838)
                     /locus_tag="ACGE4T_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8386993.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3551 a         1958 c         2296 g         3382 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttg
//
LOCUS       DAXWLG010000083        11123 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXWLG010000083 DAXWLG010000000
VERSION     DAXWLG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.084"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            90..959
                     /locus_tag="ACGE4T_001720"
     CDS             90..959
                     /locus_tag="ACGE4T_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8386994.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            979..1326
                     /locus_tag="ACGE4T_001721"
     CDS             979..1326
                     /locus_tag="ACGE4T_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386995.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1339..2601
                     /locus_tag="ACGE4T_001722"
     CDS             1339..2601
                     /locus_tag="ACGE4T_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386996.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2681..4057
                     /locus_tag="ACGE4T_001723"
     CDS             2681..4057
                     /locus_tag="ACGE4T_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8386997.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            4054..4683
                     /locus_tag="ACGE4T_001724"
     CDS             4054..4683
                     /locus_tag="ACGE4T_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386998.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4753..5181
                     /locus_tag="ACGE4T_001725"
     CDS             4753..5181
                     /locus_tag="ACGE4T_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8386999.1"
                     /translation="MGETIEYKDKNYIIIGIYNTRINRIGDPRITSDLVCQSVNYSPD
                     LTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNNEEKIFYQIYGIEKFEYE
                     HVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5740..6339
                     /locus_tag="ACGE4T_001726"
     CDS             5740..6339
                     /locus_tag="ACGE4T_001726"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8387000.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6355..6831
                     /locus_tag="ACGE4T_001727"
     CDS             6355..6831
                     /locus_tag="ACGE4T_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387001.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            6848..7750
                     /locus_tag="ACGE4T_001728"
     CDS             6848..7750
                     /locus_tag="ACGE4T_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387002.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7823..8014
                     /locus_tag="ACGE4T_001729"
     CDS             7823..8014
                     /locus_tag="ACGE4T_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387003.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            8178..8765
                     /locus_tag="ACGE4T_001730"
     CDS             8178..8765
                     /locus_tag="ACGE4T_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387004.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            8765..9664
                     /locus_tag="ACGE4T_001731"
     CDS             8765..9664
                     /locus_tag="ACGE4T_001731"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFF8387005.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            9664..10578
                     /locus_tag="ACGE4T_001732"
     CDS             9664..10578
                     /locus_tag="ACGE4T_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387006.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            10630..10845
                     /locus_tag="ACGE4T_001733"
     CDS             10630..10845
                     /locus_tag="ACGE4T_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387007.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         4275 a         1501 c         2256 g         3091 t
ORIGIN      
        1 gaataaataa aaatacattt gacaaaattt acaatatagt ttattatata tttatcttat
       61 tatgataaca caataaaagg aatggtggta tgaaaaagaa gaagtttagc cgatcgttgt
      121 taggaatttt ggttttagtt gcggttggtc ttagtggcaa gggttacttt cagaataaca
      181 gtactcagga acatgatttt cagatcaaaa gtagtgttca ggtaggtaat aaagcagaca
      241 gtgaatcaaa agaattttta gggatacgtt atgatccaaa caaatatcca aataatatcg
      301 taaacattaa tgatgggaaa tatacactga caaaagaaga ccatgaagca ttatcagaaa
      361 aaggcagtca aaccttttgg gtaaattatg cagatttaga tcaacttgga agagcaggag
      421 atgttgaagc gttgatcacc tatgatgctg taaattctca ctcatctaaa aatgtggaac
      481 gtccatcttt caaaagttct gtccatattg caggagaata caaggatgga gtatttgatc
      541 aagagagaga tacttgggtc ggcaaagatt caaacaatga aatcactcag ctagaaggct
      601 ataaaggtta tctctataac aaaagtcatt cattagcatg gtcgctagga ggagatatgg
      661 aggtacacaa tttaacactt ggaacgagag cgcaaaatgt tgggaaaaat gatcagaatg
      721 gagggatggc ttactcagaa acgattgtac gtgatgcaat ctataaaaat aagaatgaaa
      781 aagtattgta taaagttgat ccaatttata aagggaatga attagttcct cgtggttctc
      841 atgtcgaagc tttctcagtt aacgataatg gaaaaacggt aaaccttaat gtttatgtat
      901 ttaacaatca aaaaggaatc aacatcaact atcaaaatgg tacttgggta agagattaga
      961 aattgagaag gtgaagaatt ggaaacaata agcatcaatg gattagataa atcagttgca
     1021 aatcgaataa aaaaaggaaa tggctactat cttctgaaga agggtaaata ttcagttgct
     1081 agagagttag aaacaatgag tatcgtgtat gtagctaaga atcattttga cgtagaacag
     1141 gctatttttg atccagattc tgaaataggg aaatgtgttg aattatcaga gaaaactgtt
     1201 agtacaaaag gggcgatgat cattgagaga gaaatgttag acgaaatcag agtaatcatt
     1261 cctaaagata cgcagatgtg tgaggacagc ataagtgacg atgaaccaaa atcagaaaga
     1321 aaataaggag atattcagat gagcgaaaaa tccattgaat taaaggaata taagcgaagc
     1381 gatggagaaa aatattatcc tgtagccagt ttttctaaat cagtatatct ggttggacgt
     1441 gcattggtag ttggtgcaat tggcatatat attttgcatt gggtaacaaa ggttgtacaa
     1501 ctgcttgtag ataccttagt aagtattctt gtctcgttaa atgatgttaa cggaacagat
     1561 gatttaggat taaaagagat gtttaaagag ttactcaatg cgttttttcc caattattta
     1621 agttattcat ataatgtaca aagctttttt gcaaagctac acttggtaga catctctatt
     1681 ttagtattag aagcaggaac ggtattcttt gttggatatt tggttacttt gatgttcagg
     1741 catcactatg gagaacaaag accatttttt gatgatatgg aaagtagaag gttgaaaaga
     1801 gaagctctag aagctatgga tgtgaagtat gatccaaaca agattatagg agatgaaacg
     1861 aacaacaaaa aaagaaaacg aaacaaaacg caattagaat ccgatgtgct atcaacgctt
     1921 aaaaatatca aaatttctgt tcatacaaga cgtgatctaa gtgtcaatga agatattcga
     1981 gaatatatcg taaaaatccc gcaaccaaaa gggaagaaag ttagagaaac agttttacga
     2041 caaattcaag atttagggtc gatcctgaca agagcaacaa gcggacgagc caaatttggt
     2101 gaaaaagaag atgaagtttc aggcgattat ttcattttcc gagcatcagt aaatattgaa
     2161 agagaacgta aggaaagagt aagtaccaaa aacaaagaaa cggaaagagc aacatcttca
     2221 atcgcagttt caaattcaca attttctttt ccaatcaatc tctttgccga taattccaaa
     2281 aatatccaaa accaaaccga aaaagctaga aaatatgccg aaactcaaaa gcaatcttta
     2341 gaactttatc tatcttctgc tgacgtacct gcaagtctaa cagatatgca cgttgggaat
     2401 acatctattg aatatttgtt tactttatct cgtaccagaa aaattcaact aagctcaatg
     2461 aaagaagaaa ttgaaactta cttgcatata tcaggtgtaa tcgtgcggat caacgcaggg
     2521 cgtattcaaa ttactttacc attaggagaa aatgatcgta ttccaattga tgtaccatca
     2581 atgatcaata gcgtgtttta gaagacatca caaatagaaa cagaaaatac aaactaccag
     2641 ttcacaaaca gaaaagtacc gagaaaagtg aggaatagaa atggctatga aattattaga
     2701 gaaagtcaaa aagagagccg aaaaagctga atctattttg ctagataaag aacctaatat
     2761 aaagcttaat ggaaaattca gtgtcaatat gttgaacaca aaggctaaag acccaacaga
     2821 tgctattttt ggaattaagc ctgatggcac aattgctcat ttttgcatca gcgaaatgcc
     2881 acacatgttg gttgcaggaa cgacaggatc agggaaatca gtattactga atgaaattct
     2941 tgttacagta atgtgtcact caacgcctga tgaattaaaa ataggaattg tagacccaaa
     3001 aagggttgaa tttggaagat acaaaaaatt gccgttcatg ttggctgatc caattacaga
     3061 tatggatgaa gcttatgatt tttttgaata tcttgtgatc ttgatgcatg aacgttacaa
     3121 actgatggaa aaagcagggt tacaaaacat cacgctatat aacgaatatg ccgaaaaaaa
     3181 tggattagat agatttccct atgtgatctt attagttgat gaatattcac agttggttgg
     3241 aacacataaa gaagtagaag gtttgattgt tcaattagga caaatggcaa gggcagcagg
     3301 tattcatgtt atcttagcaa cacaatctcc tcgatcaaca gttgtcactg gtattatcaa
     3361 agccaatttt ccgtcacgtg tttgccttat ggtagcaagt gatttagaaa gtaggattgt
     3421 attagatgaa ggcggtggag aaagtctaag tccaaaggga gatatgctta ttaagcttgt
     3481 tgatggaagt atggttagag cgcagggagc ttacatcagt aacaaagaaa ttgaaacaat
     3541 cttcaatcac cttagaaata ctatgccaga accagaatat gttgattacc gagcaatcgt
     3601 agctgaaact cgtggtgagg gcggtgaaga cgaggatttt ggtggaattg gtaaagctcc
     3661 tgtaagaaaa tctgctaaaa gaaatttgaa cacaccttta gcaaatccat catctaaaga
     3721 gccatcaatt gaaaagaaga gtgcgtttgc tattaagctt gaaaagaaca aagaagaaaa
     3781 agtcgagcag ggagctaaaa cactagaaaa actgcaagca gaaaaactag aaaaagtttt
     3841 aagggcatta ggaaaagctg atcctgaacc gcaaacctca aacaacacga ataataaaca
     3901 gccgattgca tctaaaacca aacctaaagc taatgatcca aaaggctcag aaaacgtagt
     3961 gatcacaact gataaatctg aaaagaaaca aataaatgga agtacttcac ctatcatgca
     4021 aaaaatgtta aaagcaaaaa aacaggtgca taaatgatca tcgtcagttc ttttttaaga
     4081 tggcttactc catataaata tacagatgaa gtagttgtga aacgagcgaa gaaaagagat
     4141 aaaaaagcgg tactaactcc taaacagatg cttgatcatg gcattttagg ggtgaaggtt
     4201 ttagaagtga ataaaccgtt tgaatcaaat agtgcaagag aaaagttgct tggagctgaa
     4261 gccgatgtcg catattttta ccatgacaaa agcaagcatc aaatagctat taaattcaat
     4321 ccaaaatcgg catggcggta tcaagcaaga gatgacttga aacgtaggaa acttagacca
     4381 atgctctatc cagaaggaaa agaagcattt gatagaaccc acatcgttcc cattggtttt
     4441 catggtacag aatcagataa ccgcctatta gtaggttgga acagcaatca aaatagagtt
     4501 cagatcaata aatttgagaa gaaagtaagg aaattcaacg atcgaaaaac gatcctatgg
     4561 tttgcagata tttgtaaaca gccggatgga acagcaaagt gggaagcggt gatatttgac
     4621 gaaaatggta aaaaagtaat gtctgaaaca tggcatgata aagctaaatt tgtttggcag
     4681 tagcagtaga ggtgaaaaag attgctagaa ctttttgaag caaatagaac aggctttaat
     4741 caaaagttag aagtgggaga aactatagaa tataaagata aaaactatat tattatcgga
     4801 atctataaca cgagaattaa cagaataggt gatccacgta taaccagtga cttggtttgt
     4861 caatctgtta actattcacc tgatctgaca agcaggtata agaaatacgt gttattagaa
     4921 tatagacaca agattactga tgaaattggt gtaaacaaca cacgaaacat atttaagatc
     4981 ggaacagtga ttccttatag taacaacgaa gaaaaaatat tttatcagat ttacggaata
     5041 gaaaagtttg aatatgaaca tgtcgatctg ctagtgacgt accaaatgcg tttaatagaa
     5101 ccttggtcac aggcagaaat tgataaagca gtaaaattga accgattatc aaaatttaac
     5161 gtattaggaa atgcctttta aaaatcgcaa tacacaaaca gaaaaatacc aaaaaattag
     5221 caatgaacgt aggtaagaac atgacaataa ataataataa ccctaaaaaa tataatgctc
     5281 ttgtccaatg ctatatcgaa aacaaaatta caatgcaaga agtcgctgat gaattaggca
     5341 aatcaaagag cctagtatca cacgatttta agcacaatgt ttttccgaac ccgattgagc
     5401 tgaggaaaaa atttcaaaag agagtttatc aagaaatagg taagtggatt gatgaaggga
     5461 atgcaattga ccctttaatt gaaaaatatc cttttttaaa aagcaaaaat aaaattcaaa
     5521 ggttaattcg tcaaggcgat attcaagcta aagaacctgt tttgacaacc aatgatttgg
     5581 ctaaaataaa tcttcataaa caaattattg aagactttga aaaaaatctt tcaacaactg
     5641 aaattttaaa aaaatacaat attccgagag gaaagctaac atcgactctt tttagagaat
     5701 atggagaatc ggcagttact aaagcaaagg aagtagcgaa tgagagaaaa aaacaaaaat
     5761 agtagtcgat acctagtagc gactgtagtt tttgtttgtg tattgatttt cctgtttgat
     5821 tttttgcaga ttggaaaaag cataattgga aatacattga gtgcaaagga aatggttgct
     5881 ttaggtggtt tgtatgaagg acacttgttc ccgaatatat tcatttctat cgtttctcat
     5941 attaacttgt accatttaat ttctaattta ttgttcttag tagttcttga agaagcactg
     6001 aatgatcagt atgagtggtt aacattattg atcgttttaa tcatatcagg atttctagga
     6061 agcttagttc cttttctgct acacagcaac atcgtaagta tggggcttag tggaggtatt
     6121 tttggatgga ttgctttatt tgcgattgca tacatccgtt ttgatagcaa gaaacattta
     6181 ttactaagtg tttttgtatt attgataatc ggcattttat tggtatctac ttttgaatca
     6241 ggcgaggcaa atgtcttgtc acatgtaatc ggatttgtct gtggaatgat cttgggattt
     6301 attctaaaga acgtttcatt gatcaaacaa aagaattaaa agggagtgct aaaaatgcca
     6361 aaaagaaaag aaaagaaaat tccagtgttt ttgtctgtct cagaaatgtt atcacttcaa
     6421 atactgttaa gaaaaaagat tgaaacaata gacgaagaag ttagaaataa aagggctatg
     6481 tacgaacaac gtgaagcgct aagagaacga atccactatt ttgatgatat acacactaaa
     6541 atctatgaag cacgtacaaa tcacgaagac gaaagtaaat taggattagc tcaacaacgg
     6601 atacttgaat ggcttaatac gaatactgat caagaaccgt taagagtgat tagtaatctt
     6661 tttgaatttg gaacgaagca ttggtttaat gctgtagaga aggatgctga cttacaagaa
     6721 gcgtttgata cgctaagtaa ggatagcaaa tccatgcgag atttattgat tacttatcta
     6781 aatgagcatg agtttgaaca aacgaaaacc aaaaagaaaa ataaagaata gaaagttgtg
     6841 atgaatcatg ctattaaccc tgttaggtta tttatgttta attcttgctt tttttggggc
     6901 aatcgctata attattggtc aatttcaaac aaataatccg attatgcgta acataggcat
     6961 tggaattgtg attgtaacct ttattgtgtt taatgccact aagatttttg gaagtaacga
     7021 taaacaattt ggtatatcgg gagttaataa tagttatgct ccagattctt tgggaagggt
     7081 agtaatagat ggaacatttg aaaatgtaac gagttataaa aatgtttcgc tgacagtgaa
     7141 ggaagatggc aagaaagcat cagacgacgt tctcgctact attaagccgt ctcaatcaca
     7201 atttgaagtg ttttatacag ttaaagagaa tagccctgta cacaagctga cattggtttt
     7261 taacgttgat ggagaagaat actataaaga tgtaaaaata aataaaacat caacgaaaaa
     7321 gattatcgaa tcagcgaaaa aagaagtagc tacagaagaa agtacaactg aatcaagttc
     7381 tactgtcgaa gcggaactac cagataaaat tgattttgat cctgaaaaag tcaaatcgtt
     7441 tgaatctgtt tctggttcta tggctgatcg gtttaacact gaagtcttca aagctatcaa
     7501 aactgttaaa ttggatactg aaaagcaaat gattaatgtt accttattca attcagttga
     7561 atctatggat caggataaaa aagttacggt tgcaagagaa ctaggaaaaa gagtaattgg
     7621 tttgtatcgt ggtactgcta aaccagattc agacggtgca gatttgccag ttcatgtagt
     7681 ttatgaagac ggtaaagttt ttggagattc tacagcaaac gaagcattaa cgattaaatt
     7741 ggctaaatag ccagaaagtt ataataaaca aatggtgtca caccaataat atataggtgt
     7801 gacaccttgg agatgatagc tgatggtagt aaataaagaa aaagttagaa agtatgtgac
     7861 tttaaaaata gcttctgtta aatatttaga aaagcgagct aaagaagaag aaagagcaag
     7921 aggtaagaaa gtaacagttt cagagttgtt agaagaattg atcgagaatg atcaaacaat
     7981 aagacgtgca tttactaata ggtctatcag ataacaatat aattaataaa aaagaaagag
     8041 tgatttggtc ttagactaaa tcactctttt tatataaagt ttctttacac tcattccgaa
     8101 taatgttaaa attctttata tgatatatag ttccttgtca gaggaagaga taaccagcaa
     8161 tattggaggg ataataagtg gaaaacaata atgatattag acattttagc attggggaaa
     8221 gagttacaac agtaagtgaa ttatcaaagg attatagaga taaagaggga acgattattt
     8281 ctcttaataa gaatcgaaga ggcgtttggc aatatgttgt tgatttaggt gatggcgtta
     8341 cagttgagtg taaagatagt caactgatca gaaataatgg cagtcattcc attgaaaaag
     8401 caattcttgt atacccaaaa gtatcaagta agatcatgaa tgactggttg cttggaaaat
     8461 ttggtgagta caataaaaaa gaaatcgcag aagtgatcgg caaatcttat ccaacagtat
     8521 tagggtatct aaaaaagata acgagtaaag aagcaaaaat tgaggatgtt ttaacagtaa
     8581 gagagcttca atcattaatc gagtatcgga ataaacaaat aacaaatcct gagtacctta
     8641 gaaaagaaga tggtctattg aaacaattgg aacaagtaaa taaaatccgg aagatgcaaa
     8701 aggtagctcc tttaagcctt gataccttta aaaaaatcta tttgaaagag atcaaaaaag
     8761 actaatgtgg actgtaactt taccgttcca acctgtatct gcaccaagac caagtgcaag
     8821 aaggaatgct gctgatgaaa ctaatgacgt agcagagaaa aggatttcaa cctactatga
     8881 taaaaagtat cttgattatt tgaaaaaaat aaagagcttt atcgaagatc aagatttatt
     8941 agatgatgaa ttttactcga ttgtaaatga tcctatgggc gcaattatgg aagtggactt
     9001 ttactaccag ataccaaaaa gctataaaaa agtctataac attatgaaga caacagcgcc
     9061 agatttagat aatttagtaa aaagtgctat ggatggtatt tttaacatct cagctatacg
     9121 agatagccat atcacaggct taattgcttt taaatataat gtggcgaacg aaggaaaaac
     9181 agttgtgagg atcaaacgat attcagaatt tgagtgggat acttctgaag gattgaacgg
     9241 agatatatgg gaagctacat ttgattttaa accagtagca acgccaagac caaagtctaa
     9301 atttagagga aatgggatta tcacttacta tccaaaagag tacaacgact atttggaaaa
     9361 tatcaagaat acgcttaaag agaaagattt ggtaaatgat caactgaaaa aggtaatgaa
     9421 tgctcctatg ggggttatag ctcagataga ttatttttac caagtgagaa aagataagaa
     9481 aaagcttgat tcactgatgc gtacttcaca gccggatatt gataatttag tcaaaggaac
     9541 attggacgga atttttaaca aggaaatagg catcaaagat agcagggttg tagggttaat
     9601 tgcttttaaa tacaatactt ttgaaaagtc gaaaacaaac gtgagactac aggagatggg
     9661 ctgatgaata ataatgaaaa aattcttcat gtacttgata gctttgaaat aattcaagaa
     9721 gaactaaaaa aatatcggga tgttttggaa caaagatatg attttgtcaa tcagcaaaag
     9781 agtaatcaca tggatttcat tctcaatatg aatgacttga agaagaaatt agttgaacga
     9841 aaagaacaag aaaagttgat caaagcttac tttgaactcg gtgaaaaaga agttaaaaat
     9901 gcaatggaac tgaatgaaga gcgcagggtt ttagatcagc tgttagaaca attattggtg
     9961 atgtttcaaa aaggacgaat agatgaggat ttaattgaag aaggattgag aaaatatcct
    10021 tcaaactcag gtatcggaat agtgctgaaa gcgattgatg aagaagaaat tgaagctttc
    10081 ataccgccag aggattttga atcggcaatg gaatatataa aatattatag ccaagggatc
    10141 actgctttta gagagtttga tcctgaagat gttattcatg acttgaacaa cctaaaagaa
    10201 tggtgtgaaa gttatggagt cgatgattcc ggattggact acttgatcag tattatggaa
    10261 atcgaagaag agatgccgga taagcctgat ccaacagata ttttagaatt gatccatgaa
    10321 gctagaaatc caattgccta tatttctaga ggatatactg tattggaata ctataagcct
    10381 tatatatcag cgatgaacca tttaagacgt gtattaaggg aaaaacgtga ataccgctca
    10441 gtgttgaatg caacgaatag attagaaaaa gctgtttcag agctagatgc ttattatcgt
    10501 gaacattacc tgcaagcagg tggtatgcca agaaatacga aagcaaatat cagtaggtat
    10561 ataaaaaaag ctgagtaggc ggagttagcc cataaaatgg caaaaggtaa cctgaaaggg
    10621 gcattttgaa tgaaaaggga tgtttttgaa ttgtacgtat tagaacgtct agaagacggc
    10681 atgatggcac aagagataat tgatgaaatt gatgatatga tcagcagatt tggtcaattg
    10741 gagttgaatg atggaaacaa aaattcggta gtgctaaaac aattttggtt gaacaatttt
    10801 tcgtataagg attttatcaa agaaaaggca aatacattgt actgactaaa ttcacgagga
    10861 gcagtgatga gagcttattg ctaagagcag ttaggctctt ttttgtttgt gtaaaattat
    10921 ttgttttata atacacattt atacacaaaa aaatattgac agatagtaca caaatgtggt
    10981 aggatatatt tgtaagcaag ttagattaac aagttaaagg aagagcagtt atgaaaaaag
    11041 aagaactaat agatatattt caaattgttg aacgagcaaa taatatggga attatgtttt
    11101 ttgatcgtat ttctttaaag atg
//
LOCUS       DAXWLG010000084        10995 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXWLG010000084 DAXWLG010000000
VERSION     DAXWLG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.085"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(357..3068)
                     /locus_tag="ACGE4T_001734"
     CDS             complement(357..3068)
                     /locus_tag="ACGE4T_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFF8387008.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(3078..3779)
                     /locus_tag="ACGE4T_001735"
     CDS             complement(3078..3779)
                     /locus_tag="ACGE4T_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387009.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3965..4564
                     /locus_tag="ACGE4T_001736"
     CDS             3965..4564
                     /locus_tag="ACGE4T_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8387010.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4645..5658
                     /locus_tag="ACGE4T_001737"
     CDS             4645..5658
                     /locus_tag="ACGE4T_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFF8387011.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            5766..6683
                     /gene="rbsK"
                     /locus_tag="ACGE4T_001738"
     CDS             5766..6683
                     /gene="rbsK"
                     /locus_tag="ACGE4T_001738"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFF8387012.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            6711..7106
                     /gene="rbsD"
                     /locus_tag="ACGE4T_001739"
     CDS             6711..7106
                     /gene="rbsD"
                     /locus_tag="ACGE4T_001739"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFF8387013.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            7106..8479
                     /locus_tag="ACGE4T_001740"
     CDS             7106..8479
                     /locus_tag="ACGE4T_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8387014.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            8836..10980
                     /locus_tag="ACGE4T_001741"
     CDS             8836..10980
                     /locus_tag="ACGE4T_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8387015.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         3404 a         2193 c         2093 g         3305 t
ORIGIN      
        1 aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag agatcagaca
       61 tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc tataaatggt
      121 gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg aggagctaat
      181 tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct ctgattcatg
      241 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aaaaactatt
      301 ttgccgtttg tttctctaat actcaggtca aaccgctgat ttcacaacct ctgtttttat
      361 tcattcgatt tcaacgcatc aatcatatcg atatgcttta atttgaaatg ggtaacaatc
      421 atcacaataa ccgtaaatac gatagttaat cctgctgata agacataagc ccaccatgtg
      481 atcactaatg gaaagatgac attctccata gacgcttgtt gcaagatgaa gtcagtcaat
      541 atatatccta caccaaatcc tccgatgatt cctaaaagag taaagatgat attttctcga
      601 acaatgtaca tcgtgacttc tttatcaaaa aatccaagga cttttattgt agataattcg
      661 cgtacacgct cggaaatatt gatattagtt agattataca agacgataaa agccagcagt
      721 cctgataata cgacaaagat taagacaacc gagtctaagt tcgccattga ttcttcttgc
      781 gtttcgattt gtttggacat gaaagacgta tttgtcacag cgtctgtggc aagcagtttc
      841 tcggaaagca tttcttcttc ttttgttgtc atcttatctg tacgcagcaa aaatgcatta
      901 tccatcatcg attgatcagt aactgaagca taatattcag gggacatata aataaagcta
      961 cctagatagt ttttcacgat ttcttttact tcgactttcc attctttttg atcttcatca
     1021 taaagcgaaa tatgatctcc tgtagataag tcaaatagtt ctgctacttt ttgcgtaata
     1081 gcgattcctg ttttaggcaa ggagcgtggg ttctcttcaa gatcagttaa atggataaaa
     1141 cgatccaaat cagctgtttt acttggtacg atcactgtca gactttgcga agcatgtcct
     1201 ttttttctca attctacggt ttgagtatga atagctagga tctcatttac tttttcttgc
     1261 ttctgcagca tatccgctgc tttttgacga ctatcttccg tggatgaatc caatgtaacg
     1321 attgcttgat agtcaatgat cggtccaaat tgtttggctg aaacagaact tagagaatct
     1381 ttcaatccca cgcctgcaac catcagcccg gcacatccag caattccgat aatcgccata
     1441 agcattcttg acttatatcg gaagagattt cggtaactga ttttttgatt aaagctaagg
     1501 cgtgaccaca gcggagtgat gtattcaaga aaaatgcgtt tccctggctt cggtgctttc
     1561 ggttgaagca atgcagcagg ccgttctctt aaatctttga ataaaacgat cattgcagcg
     1621 ccaaatgcgg caatgaagaa tgcggcagct gcttgtaaaa tcggcggcca gtcataaaaa
     1681 ataagcgcac tcccaatatc ataccgctcg ttagacagct caaaaatgat ccttggcaaa
     1741 agattcgttc ctaatatcgt tcctaaaaca atacctatcc ctgaggctag acttgcatag
     1801 attgcatatt tttttgcgat ttcaactttc gtataaccta acgcctttaa tgtcccgatt
     1861 tccctgcggt tttcttctac catacgtgtc attgtcgtaa aggtgatcaa tgcggcaata
     1921 aagaaaaaga aaacaggaaa aacattggca atcgcagcga tacgatcaga cagatcacca
     1981 aattcttgaa aaccgacatt ttctgaacgt tcattgtata ggtatgatgg tttagttaaa
     2041 ttttcaattt tttctgcatt tcgattcaat tcttcctctg cagaggccag ttcacgttct
     2101 ttctcaacta attgcctttc ttgttcagca agttgagctt cttgttgaga taatgtttga
     2161 acagcgattg ttcgttgcgc ttcaggaagc tgctggatag ttgctgactg ttgcgctaac
     2221 tgttttttag catcttctaa ccgtgttctt gcttgcgttg tttgtgcttt gccatccgct
     2281 attttttgct gtgccggttc taattcttcg ttggccgatt ttttcagctc ttctaagcgt
     2341 tcttcgggtc gatcttgtaa atacttttct gccttttctt gattttgctc cattttatct
     2401 ttgtaagctt tgctgtacgt attcaaattc tttacgtttg taaaatcaag atataagaca
     2461 gaataaatat ctgttttaaa aacggattca ggtaaataga caaaataatc gacgctacca
     2521 tttccgacgt tcgcgtatcc tctctccaaa ttgttgatga acaatggaga acggacaaag
     2581 cccgtgatgg tatactcttt ttctttcaat tcatctgaat caatctgata aatatcaccg
     2641 attttatacc cttcatcttc cgctttttca tccaatacaa gctggtttgg tttttccggg
     2701 agttttccat cagttaattg tagcttgttc tgcttgttat ttttgttata agaaaatatt
     2761 tgaatcactt cgtttttgcc agtattggaa taatagatct gttttcctgt ttctactttt
     2821 gcatctggga tcttttctgc ttgagcaata tctttttgtg tcaagccacc agtagaaacg
     2881 atttgcaaat cagaaagtcc tgctgtacca acatagtgat ccatcgtttt ttgcatgaca
     2941 ggtccagcag ttttgacacc tacaaataaa agtgttccta gcatgacaat aaggatgatt
     3001 gcaatgaatc gtcctttaga ttgccgaatt tcgcgaaaac ctgctttaag atatgttctt
     3061 gttttcataa aatcccccta ccatacaatc tcatcaatgg atgaaggatg atcatttaat
     3121 tcaacagacc ttactttagc atcattaatg tgaatgactc tatcagcgat gggcgctaaa
     3181 gcagaattat gtgtaatgat caaaacggta ttgccgtgtt gtctgctagc gttttgcaat
     3241 aatttcaata cttgcttgcc cgtttcaaaa tccagcgcgc ctgtcggttc atcacataaa
     3301 agaagtttgg gatttttagc tagtgcacga gcaattgaaa ccctctgctg ttcgcctcct
     3361 gataattggg acggaaagtt attcaatcga tgagctaggc ccacttgcct caatacctct
     3421 acaggatcaa gcgcatcagg agaaacttcc gttgccaatt ccacattttc ttttgctgtt
     3481 agattaggaa cgagattgta gaattggaaa acgaacccta catctgttcg tcggtaagca
     3541 gtaagctgtt tatctgaaaa ttgcgcaata tctgtgtcat caataatgat ctgaccttca
     3601 tcaggtgtat ccatcccacc taaaatatta agaatcgttg actttcctgc tccacttgga
     3661 ccaagtataa tcactaattc gcctttttca attgaaaaag aaatatcgtc atttgctgta
     3721 atggtcgttt ctcccatttt gtatcgtttg tattcatttt ttacttcgac atacttcact
     3781 ctgattcctc cattttcacg atatttctcg ctatatctat gcatcgaatg cgttttctca
     3841 cacttattta ttttaccact gaatttctct agactaaaga aaggaatcct ttaatttagt
     3901 tgacaaataa acgatttatg atttacttac ataaaaaaca ttgttatttg aaagaaggaa
     3961 catcatgaat gatttaaagg aaaatatgat ttttgattta tcaaaaactg cttttgtcgc
     4021 gatcgacatg caaaaagaga tcgtaaataa cggtacactc tctccacaca ctgcagcatc
     4081 tattctgaca aacaatgatt tgttagtaag aacattgaaa cgtaccgctg ccttgaaagt
     4141 tttagtgaac gtagacatct ccactttccc ttatttgagt caacaaactg atatgggtgg
     4201 aatgactgct tctgtacctc cagaattcac tgatttactt ttgaaggatt ctttgaaaga
     4261 tacagataat atgttgacta tcacaaaata caatccaagt gctttttttg gtacttctct
     4321 tgatcttcaa cttcgccggc gtggaatcga aactatcatt ttaagcggtg tagcgacaac
     4381 aaacggtgta tatgctactg cattagatgc ctttcaacac gggtaccata tcgtgcttgc
     4441 tgaagatgct tgcagtgatc gtgacaaaga atcccatcaa ttgttcataa aaaagatatt
     4501 tccgaaaact gcacgagtac gctcaacgaa acaaataatc gaagctatcc aacaaagcaa
     4561 ataagtactg gcacacgtga ttttcattta cttctttttg atttcatggg aaaataacca
     4621 caggatttag ataggagtat gaacatggtg aaaaaaaaga tcacaatcaa agaagtcgct
     4681 aaactttccg gcgtttcaat cacaactgtc tccttgatat taaatggaaa tacggagaaa
     4741 ttcagtccga aaactgttca gaaagtttta gctgccaaag aagaattagg ctatcagcct
     4801 gactattttg ctagacaaat gattaccaag gaaacgaaaa caattggtgt actcgttcct
     4861 gatatcacga atccattctt cagtacactg atgcgaggaa ttgaggatat cttgtataag
     4921 cagaatttcg tcacgatatt atgtaatgct gattctgatc atcaaaaaga aatcgaatat
     4981 ctagccgagc tcacacgtcg aggtgtggat ggattcatta tcgcgacttc tgctgtctct
     5041 acagatgcaa tcaacgaaaa tttgaaaaaa caaggccggc ccttcattgt tttggatcag
     5101 aaaaaatctg aaggatttag tgatgccgta cgaacagacg attttcgcgg tggttaccta
     5161 gcgggcatgc acttgcttag tctaggacat caaacgatcg cccttgtcta tcctgaaaat
     5221 ccaccggaaa atgtccacgc tcggattgag gggtttaaat ctgctcttga tgtttaccaa
     5281 atccctcacg accaactgat cctcttacct acacagttct caaaacaagg tggctaccag
     5341 atcacagcag aacttcttga ttctgctgca actggtgtgt ttgctttaaa tgatgaatta
     5401 gcttttggat tatatcgcgg attagaggaa gctggaaaaa gcatccctga agactacagc
     5461 attattggct atgataatat agatatgtgc gagtatatca agccgaaact gacaacgatc
     5521 gcacaaccaa tcttcgaatt gggacaaaca tctgccaagt tattgttaga tcggatccaa
     5581 ttcccagaaa aagaatggga agaaaaaaga ttacctgtac gattagaaaa aagattttca
     5641 actgcaccct tgaaatagct ttcaaggtgt gctatatttt tatcgaacta ttaaaacgtt
     5701 ttactaaaac gttttaaaaa ataattttcc catcaaaata aatgagatag ttggaggttt
     5761 gtaaaatgaa cacggttaca gtaattggaa gtattaatat ggacactacc cttcgtttaa
     5821 ccaacatgcc caagccaggc gaaacgatgc atgcgcatga gatttttcac gctggcggag
     5881 gtaaaggtgc caaccaagca gtggcagcca aacgttcagg cgcacggaca tcttttattg
     5941 gaggcgttgg ggctgacagc gaaggacaac agttattaga tctactgaca aaagaaaaca
     6001 tcgatacatc tgggatagct gaaatccaag gcgccacaac cggacaagca atgattatgg
     6061 tagacgcagc aggagaaaat agtatcttga tccatgcagg ggcaaataat gctttccatg
     6121 aacaagaagt cctaaaaaac aagcaactga ttacaaacag tgactttatt attgctcagt
     6181 ttgaaagtag cttagatgcg acgatcctag ctttttctat tgcaaaagac gcaggaaaaa
     6241 caacgatcct gaatcctgca ccagctcgtg aaacgattcc tactgaacta cttgaaaaga
     6301 cagatatcat tataccaaat gaaacagaaa cagaaatcat tacaggaatc agagtgacag
     6361 atcataacag tttggtcgca gcagccgaaa aactccatga attaggtatt ggaacagtaa
     6421 tcattacact aggcagtgcc ggcgctttct atcatacaga aaaagaacac ggtatcgtcc
     6481 ctgcttttaa agtggatgcc gttgacacaa cagccgcagg tgatacattt attggcgcat
     6541 taagcagcac acttcagcca gaccttagta acttgaaaga agcgatcctt tatggaaatc
     6601 tagcctcttc tgttgctgtg cagtcttatg gtgcgcaacc ttctatccct taccgcgaag
     6661 ctttagaaca atcagcagtc taaaattaac aatgagagga agaaacaaac atgaaaaaag
     6721 gaaaagtaat caattcagat atttcacgag taatcgcaca aatggggcac tttgataaat
     6781 taagtatcgg ggatgcaggc atgcctgtcc cctttggtac tgaaaaaata gatttggctg
     6841 tctcaaacgg tcttccaagt tttttatctg ttttagaaaa cgttttagaa gaacttgagg
     6901 tacaatcgat tgaattggcg gaagaaataa agacaatgaa cccggaaata ttggaaaaaa
     6961 tcaaattatt gttaccagat acaccgatta gctttatccc acatgaagaa atgaaaaaag
     7021 agttgaataa ctgtcatgca tttattcgta caggcgaaat gacaccttac gccaatatta
     7081 tattagtgag tggagtggtc ttctaatgga aacgacaatg aatcctgaac agatggtact
     7141 gtccgagcag attcatatgc tgattgaaca gttgggagga aaagacaata ttgattctgt
     7201 ccttccatgt gcgactcggc ttcgtatcaa attgaaaaaa cagccagtag ccaatgcaga
     7261 aaaattaaaa cagcaaatcc cttccattaa gtatttcatt ttactcgata gatatgcaga
     7321 aataatcatg gatcagggat tggctcctgc ttacaaggaa ttgacgcgtc aaatcgatca
     7381 ttcagctccg aaagaaaagc taaatccgat cgaaaaacta tttggtttga tatcggaaat
     7441 ctttacgccg atcattccgc tgtttgccgg ttctggtata ttaaaaggtc tggttgtttt
     7501 agcgactacg attggctggc ttagtgaaac aagcggaact tatcatattt tgtctgctgc
     7561 gtcgaatgcc gtcttttatt ttctgccaat ctttcttgct ttttctgctg ccaaaacgtt
     7621 taaagtgaat ggatatattg cggctgtcat tgctgcagca ctgattgaac cgaatctgac
     7681 ctctttactt tcagacagtg gcaaagcagc tactgatttt ctggggattc ctgtagtact
     7741 gatgcagtat acgtctactg tcatgccggc gattttaggt attttctttt attcttttgt
     7801 tgaaaaattc ctcaaaaaat atattcgcga aaatatgcaa ttagtatttg tacctttact
     7861 ttcattagtt atcacggttc ctttgacgtt aatggtattc gggcctgcgg gtgtttacct
     7921 tggcgaaggt ttagctgccg caattacttg gatgatggat attaacggac cgatcagtgg
     7981 cgcaatcatc gctggtggtt ggaatatttt ggtaatattt ggtatccaat gggctgtaaa
     8041 ccctgtcatg atcagtaata tctctgctta tgggttcgac cgaatcgttc cgttgacagg
     8101 agcagccaat tttggaatgg ctggtgcagc tttaggtgta tttctgcgaa gtaaacgttc
     8161 aaaaacccgt tcgatctccg gttctgcttt tgcttcgatt ttacttgcgg gagtgaccga
     8221 accaactgtt tacggaattg cgatcccatt gaaaaaacca tttgttgctg cttgcattgg
     8281 tgcagctgct ggtggagctg taatgggatt tgcccaagtg aaagcgatcg cttttgtatt
     8341 tggtagtctg accacattgc ctgcattcat ttccggtact tttttctggt acttagcagg
     8401 actggctgtt agtctagtcg tagcaatgat cactactctc gttagcgggt ttgatgaaga
     8461 cctaatgtct tatgaatagt taatgaaaaa gactgaagta tgagatgttt atcacatatt
     8521 tcagtctttt tctttttagg atcattattt ttataaattt ttgtgatact ttccaaactt
     8581 tggtataatt tacatatgca taaccatttt tatccccact tgtttatccc caaacaaagg
     8641 tggtctatgc aataaaatga acgtcaaaga aaaacgaatg aatgctcgtt gcttatttac
     8701 aaattgtttt atttagaata cacagacaga tttatgaaaa gaaaaagctt ttcgacacga
     8761 ttatctgttc caaaaaatgt ctaatgaatg ctgaataaga cagaaaatca atgaaaggaa
     8821 aaggactagt ttattatgaa aaataaaaaa acgatacgtt tgctagctgc tacttggctg
     8881 attgctccgt ctgttttgtc gaccaatacc gtatttgccg tagataatca aatagctgat
     8941 caaacagtca caacagatac gactacatcc agctcagcta ctagtgaaac aacggattct
     9001 tttacagaag acccaactgg aacaactaac tcaacaacag gatcgaccga gccaactgaa
     9061 attgatccgg ctgatccatc agaaaatgaa aagccagccg agacatattc ccttacaatt
     9121 gctccttctt tgattggtaa gatccaactg actgcttcga cagcggattc ccctgaacag
     9181 caacctttgg atattacgga aagtggaacg gtagaaggtt tattagcgga aacgaaggta
     9241 acttacacta ttacgccagc tgaaggtgaa caaattgctt cagtcgctgt taatggtgcc
     9301 aaagaagtaa attatgatgc aaagaccttt acagtaggta cgaacaattt atctattact
     9361 gcagaattta aagctggacc ggatacttca acagagaaac caactgatcc tagtgaagaa
     9421 gtccctagta caccagatcc ggaaacgcca aacaaaccag atgtgggtaa taacaacaat
     9481 aataatggtt caaataattc aaacaataat aacaataatt cgaacagcgg gaattcttcg
     9541 aatcaaacaa acccatctgg atcgaataac aatggaaata ctcagagaga accaaaccat
     9601 ccaaacaatg gttcatcgaa tggcggaacg aacaatgctt catctcgtcc aaatacagca
     9661 aatcaaagcg gtatagagaa tccatcttct gcttcttctg attttgttgt aaaatcacca
     9721 attgatgctg tattgcctac aaatacaacg aatgtccaac aagcaatcgt tcgagaagca
     9781 ttcaagcatc ttggaaaacc atatgtatgg ggagcaaaag gtccaaatac ttttgactgt
     9841 tctggtttga cttattacgt atatatgaaa gcaaccggtc attatatcgg tggctggaca
     9901 ggcgaacagc aatatgcagg tacgcagatt ccagtaagtc aggcccaacc tggcgacctc
     9961 gtcttctggg gaccttcttc cggtgttact caccatgtag gtatctatat tgggaacgga
    10021 caatttatcc atgcgcctca accaggagat aaagttcgag taacttctat ttcagatttc
    10081 acgcctgatt ttgctgtccg agtaaactta gcaggtttac ctgctgcttc aggttcgctg
    10141 acaggcggct catctatttt agatggatta gatggctcgt tccatttcag tcaaaaccaa
    10201 acaactgatc agttcttgaa aaagattgca gatgatgcac aggaaatcgg gcaaaaagaa
    10261 ggaatctacg catcagtcat gatggctcaa gctatccttg aaagtggatc tggaaacagc
    10321 ttattatcaa gcgaaccaaa tcataacctt tttggtatca aaggatcata taaaggaagc
    10381 agcgtgacct tcaatacact tgaacaagat agttccggac aaagctacca aatccgcgca
    10441 caattccgta aatatccttc ttacaaggaa agtttggaag actatgccga cttgatcaaa
    10501 aatggactga ctggcaatcc tgatttctac aaaccaactt ggaagtcgga aacaaaagac
    10561 tacaaagaag caaccaaata tttggaagga cgttatgcta cagatcgcca atattcacaa
    10621 aaattgaacg cgatcatcga agcgtacgac ctgacaaaat atgatgaacc gaaaaaagaa
    10681 gcagcaacaa ctgaagaagc aaacgctgaa atcctcaata tgtctaagcg attcgatgtg
    10741 ccaatcaaat ggacaacaga tggcgtgcca atcacaggat tagaattttc aacgataaaa
    10801 cgcgtcaaca acgcttcttc catcttccgt gtcgcttcaa tctgggattt gtggaacaat
    10861 tttacggcaa gacagatacc agaaacaact actcgtacag ttaaagctgc aactgttcct
    10921 gtactaacct tgttaagttt agacaaatca ttctggcttg atctgccttt aaagaaatag
    10981 aataaattaa aaagg
//
LOCUS       DAXWLG010000085        10977 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXWLG010000085 DAXWLG010000000
VERSION     DAXWLG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.086"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(896..1408)
                     /locus_tag="ACGE4T_001742"
     CDS             complement(896..1408)
                     /locus_tag="ACGE4T_001742"
                     /inference="COORDINATES: protein motif:HMM:NF042930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFF8387016.1"
                     /translation="MKNYYFFFLSCFCLFGLTSCGWIFSEEKTEVSLISKEKMIQAES
                     SSKESKRNQEDKTECNISEEIQYLLKNFGRKWVNYTDIYERNQSVKNYLTDRCIKENG
                     IDNDPHVQYKTIGRIHTITQDVIQSDQYLLFGEETTNEMTRFVLLQIKVDQEVQKIDK
                     IKIYYVRSAY"
     gene            complement(1459..2517)
                     /locus_tag="ACGE4T_001743"
     CDS             complement(1459..2517)
                     /locus_tag="ACGE4T_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8387017.1"
                     /translation="MMSCLFLLFAIISMSSGESKHMDSAVGVTHTGEYIETLPIFSEI
                     KGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRNDTNFFGITWFPG
                     CPYPQGSARGIGGSEGGFYMKFPNLNACYDYYGFMVASQSNFNQCVGNKDPGSCLLIL
                     GRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDAFAIKHWNSLPTKKTSLIAGKSKGN
                     AKVLDRIIGRPVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGEDYNWATYGWQVI
                     MNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSNSGQSFSTVEQNAEQGRIMAR
                     YQRSYNQAKIRSLVRKVK"
     gene            complement(2550..3338)
                     /locus_tag="ACGE4T_001744"
     CDS             complement(2550..3338)
                     /locus_tag="ACGE4T_001744"
                     /inference="COORDINATES: protein motif:HMM:NF024056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8387018.1"
                     /translation="MVFSVCFGVYNNLTSQDTHTIHETKVIDKQLKDMTGIESYVKNF
                     AFLYFTVSLESDQQINRSKAVESYLADGLQMDNEIDKDIETNVKVKNVSIWAIEEHES
                     KKHTFVVIFHVLLEIEKEFKERVYKVEVYRNELSYAIVKLPIVASNVKKATIKVDTRF
                     ETTSISRKEQEELEKFLYTFFRIYPKANAEELKYYGTTLLPIQQPLAFEELSNLQVAK
                     KEGRYEIHCNILYQDQETHLWFTMYYEMSMKQLENGNFRIEAIH"
     gene            complement(3485..5506)
                     /locus_tag="ACGE4T_001745"
     CDS             complement(3485..5506)
                     /locus_tag="ACGE4T_001745"
                     /inference="COORDINATES: protein motif:HMM:NF046089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8387019.1"
                     /translation="MKQWYSKVSFSITRRKLVFISLVIMMGVFFFHPIFVEAAGWIKE
                     TLGGKNAVYDFETYPLDNYNLDFFVDTSWNWLPWKWGEGISNTVIYGIFAVTNILWML
                     NVYLCYFLGFLVQEAFELDFLSELIGSLSHSIQNIAGMNRAGTLSHGLVPFFGGLLIA
                     LAGCSVVYQGIVKQHPTQAIQQAVTFLFTFICCGIFFMNTHTYLEKANEVQKEINSEM
                     LTIAKDLLPEQKGKPDAIKAIRENLFAIQIKQPWLVLQFGDSDIKTVGEERVINLLKE
                     SPYDEGEQRNKLVEEEVTKQKNGNLSVQKVYLRFGMVFLTLIVNIVISMGVLALVVIL
                     IASQVLFLLFIGFLPVAMIFSLFPNSGSLLGNALQKVFQLLFTKMGILLILTITFSIS
                     HELNHLSKNKGMIWTSFLQVTLWFSVSTRVNELLGWMKIGGAQTRAGDRTGRFLRGMM
                     LGTMGQKMIRGIGENFVGGAAGGVADQMISKSMSGQRSITTTPLIERVGKKVSAMTEL
                     PATIESRAEQWKRTIQYVPTNAQYKAQKMKYDYFSGRMNESERQTRARNLANERQKNF
                     YEARKDALQLEQQERKQQAMRSMAHQTGKTQVTSGKLQTRKSAMIGRTEQFPTIEKRS
                     NISNVAKNKKLSKQEHPIHERSTNRKIETPVRWTSTSCQNARKGRTKKE"
     gene            complement(5507..8044)
                     /locus_tag="ACGE4T_001746"
     CDS             complement(5507..8044)
                     /locus_tag="ACGE4T_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8387020.1"
                     /translation="MRLPKLVPKIPFYYLEDNLVFGEEDRVYAYYEFPMYSYSFVGED
                     QAYQLQQAIMRLIRQSYSSHVRFFFCTNEEKIEKTIKASKELVRSEGELKTIAYQHLE
                     GVQHILEELHGKYIQNVCWYVGIELVLEESAPKEAHWMKEFRIATKDFFHRSNARLFG
                     DFETIPNEKIQRYKRLENLLYNRISKYFRLRKTTPEDMVFLVEHLEGKKEQSLDEVMY
                     RPNSFKEETNTYTYHYDVLNLASAGITEKKNYLILEQGTEKEYITYLSLSRMTRDLVF
                     PFQSEIYYFLQSELDFPIDSWLDVNLLDNRLALAKVRGKKSDLSDIYESGMSVGKDVG
                     NNVREAYQSANELEQKLEDTREDLYVLSLNVRVSGKTYDEMLKRVSDVRNFFDVYSMY
                     LEVPCNVQLFLHFGSYPAGRRVIEDYRQYVGAEFIASLGIGATQQLGDGCGIPIGFNA
                     ETGIPVFIQPWLAAQGIKGTVTNTLAKALIGSLGGGKSLTENLLLFWSVLFGALAFCI
                     DPKSERSNWSEDLPYFAPYLKTVNITSEKENIGLLDPFQLMRRKKDQESLALDVLTYI
                     TGVSIRDEDRFPLLQRAVKTVSDFEGTKGLLLVIQELYAQEAAIAQRLAKHIESFKEL
                     SIAGLLFGDGTNQQGLNLDAKCNLALVQDLTLPDSETAPEDYNSSEILSIAIMMSLAT
                     YALDFIKQNRDIYKAVGLDESWAWLNVAQGKILGNKLVREGRSRNAGIDFSTQNTDDL
                     GDEKMRSKIGLKFIFRSNDREEITKALNFCGISVTEENIQRVKNLENGECLFCDIHGN
                     IGILYVYYWFEDLFHAFDTRPPMEKNYEEEEYKDEILEKLVEEIEVN"
     gene            complement(8066..8425)
                     /locus_tag="ACGE4T_001747"
     CDS             complement(8066..8425)
                     /locus_tag="ACGE4T_001747"
                     /inference="COORDINATES: protein motif:HMM:NF024062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFF8387021.1"
                     /translation="MNKIRNWTKIWYIERVFYKLWDGVALPRPVTQTFAIWFGIGFFG
                     GIFRIFPFVMEHTITRQFFCPWLFAFLMTRKQFQGRKPYNYLASFLLFQCRSKESFHG
                     QWVKPIEKKKSNEEFVV"
     gene            complement(8427..8645)
                     /locus_tag="ACGE4T_001748"
     CDS             complement(8427..8645)
                     /locus_tag="ACGE4T_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387022.1"
                     /translation="MNFFQNVFTDFVGDFKYLIMLAMLGGAAYIAFERKVSKAIPLIL
                     VLAVAVWLVGDTSGVFNWILDRMRSWGR"
     gene            complement(9167..10147)
                     /locus_tag="ACGE4T_001749"
     CDS             complement(9167..10147)
                     /locus_tag="ACGE4T_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8387023.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFDRASRFAEEMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVNKAKENNLGKLIY"
     gene            complement(10160..>10977)
                     /locus_tag="ACGE4T_001750"
     CDS             complement(10160..>10977)
                     /locus_tag="ACGE4T_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8387024.1"
                     /translation="LRDLFKNKQNFKEEPGDMRLLPFTSFIIFFLSTFGVSDFAIGTV
                     LYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAINVGIKTLAVCIICQIIGAYLGP
                     RFVVKLPEKTIKVFVGIGLVIAALLIFAGQMNWIPSNGTASELYGGKLILAGFLLFVY
                     GALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACTFSVPIGSVQFIKFNEYSRKIT
                     LFTSTFGVLGVLVAVFLVKSLDTYVLKWIVILVLLYSAYTMLGSQLKKVSAAN"
BASE COUNT         3300 a         2344 c         1651 g         3682 t
ORIGIN      
        1 aaacaatgtt aacaagaagg agctactaag tcaaataaaa tgaacgttca atttgttttt
       61 ttaactcgag cattttttca tgaaaaatca aacaatcaat tataaaagac acagcactat
      121 ctttcttatt caaaactagt aaacattgta gcttctatat ttgtcaagaa aggatcataa
      181 tttgttcgga aatgaaaaat actctattta gaattttttt cgataaaaaa taaaagtata
      241 aattattttt ctgaaagttt taatggtaat gaacgttaat tttataaggt aaaagctatg
      301 agtgtaagag caaatttgtt gagataacgt gtaggtattc ttataataga cttgggaata
      361 ttcctatatc actctctagg ggtagctcag gggaaggagt tgccccttta aatgtatatt
      421 agaataatca agataaccag gtgctttgaa attctgaatc tatttatcca attcaactgt
      481 gtttcctata gagtttatac aggaacccat cgatcataat caattgttct gaaattacat
      541 ctaactgatt ataaatttgc tctacaagct catccgcttt tgtatgtagc gatagaaatt
      601 tgcgtccgtg tatctactta ggatcatttt ttcacaaatt catcccatga ttcactaaat
      661 catggttcaa aaagacattt ttcaactcta gtacttgttt taaccaaagt acttctactt
      721 tttccgtttc tatctcattc ctcctcctat tttttgtaca ttattttctt atctctttcc
      781 ttttaattga tttattccca aaattcttca tattctttaa acatcttaaa tagttcagct
      841 tcttcctttt cgttaaacta taatttctag taaataaaac aacgtcattt ttcttttaat
      901 atgcacttct tacataataa atcttgattt tatcaatctt ctgcacttct tgatccacct
      961 tgatttgaag taacacaaac cgagtcatct catttgttgt ttcttctcca aataataaat
     1021 attgatccga ttggattaca tcttgtgtga tagtatgaat acggccaatt gttttatatt
     1081 gtacatgtgg atcgttgtca atcccatttt cttttataca acgatcggtt aaatagtttt
     1141 ttacagattg atttcgttcg tatatatccg tgtaattcac ccattttctt ccaaaattct
     1201 ttaacaaata ttggatttct tcagaaatat tgcattctgt tttgtcttcc tgatttcttt
     1261 tgctttcttt acttgaactt tctgcttgta tcatcttttc ttttgatatt aaggaaactt
     1321 ctgttttttc ttcagaaaaa atccacccac aactagtaag tccaaacaaa caaaaacaac
     1381 ttagaaagaa aaaataataa tttttcacaa tcgatccttc tcccttttat actaaaaatt
     1441 ttcatcgaaa gaattctttt atttcacttt ccgaaccaga cttcgaattt tcgcttggtt
     1501 atagctgcgt tgataccgtg ccataattcg cccctgctcg gcgttctgtt ctacggtgga
     1561 gaaactttgt ccactatttg agacatctgt tatgataccc gtgtggccat agattccagg
     1621 agacaatgca ccacctgctt gccaattgac cacatctcct gcttgtaagt cactgggctt
     1681 tggattcatg atcacttgcc aaccataggt tgcccagtta taatcttccc ctattaacat
     1741 cgcaaacatg tgtccagaac ccattaactg tgggccacct agttgagata cataccatgc
     1801 cgtaccacca taacattccc cattatttac gggtcgtcca atgatccgat ccaaaacctt
     1861 cgcattgcct ttactttttc ctgcaataag cgaagttttt ttggttggta ggctattcca
     1921 atgcttgatc gcaaaggcat catattccgt taagttatag gactttatga tactcattgc
     1981 gccttgataa tacggactgc cttcggtgat acctgccgcc gcataacctc cacgacctag
     2041 aatcaataaa caacttccag gatctttatt gcccacacat tgattaaagt tgctttgact
     2101 cgccaccata aatccataat agtcataaca agcattcagg ttcggaaact tcatatagaa
     2161 accaccttca gagccaccaa tcccacgtgc agaaccttgt ggatagggac aaccaggaaa
     2221 ccacgtaatc ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgcc cataagctga
     2281 ttcatatcct aattgagaaa gaatcacact cggtaataac tgatacttta cagctgcacc
     2341 aactgctgct tgggcatgtt catctgttat cggaccacgc cctttgatct ctgaaaaaat
     2401 aggtaaggtt tcgatatatt ctcccgtatg agtaacacca actgctgaat ccatgtgctt
     2461 cgattcacca ctagacatgc taattatcgc aaataataaa aacaggcaac tcatcataca
     2521 gatgccacta aataaaaaaa tctttttcac tagtgaatcg cctcaattcg aaagttccca
     2581 ttctctaatt gtttcataga catttcatag tacatggtaa accaaaggtg cgtttcttga
     2641 tcttgataca aaatattaca atgaatctca tatctaccct ctttttttgc gacttgcaaa
     2701 ttcgatagtt cttcaaaggc taaaggctgt tgaattggta aaagagttgt cccataatat
     2761 tttaattctt ctgcgtttgc tttaggataa atacgaaaga aggtatataa gaatttctct
     2821 aattcttcct gttcttttcg agaaatgctc gttgtttcga accgagtgtc tactttaatc
     2881 gttgcttttt taacattact cgctacaata ggcaatttca ctatggcata tgataattcg
     2941 tttcgatata cctccacctt atacacacgc tctttgaatt ctttttctat ctctaataag
     3001 acatgaaata taaccacaaa tgtatgtttt tttgattcat gttcctctat tgcccaaatc
     3061 gagacatttt taacttttac attcgtttct atgtctttat ctatttcatt atccatttgc
     3121 agcccatcag ctaaataact ttctactgct tttgatcgat ttatttgttg gtcgctttca
     3181 agactcactg taaaatacaa aaatgcaaaa tttttcacat aactttctat tccagtcata
     3241 tcttttaatt gtttatcaat gacttttgtt tcatggattg tatgcgtatc ttgagaagtt
     3301 aaattattat aaactccaaa acaaacacta aaaaccaaaa agctccagcc tacaaaaaaa
     3361 aggaagcgcc tttttcgcgc tcccttatag ctttcttttt tcttgtcttt ctttcctttt
     3421 agtcttgttt cttgttcaga gagaatagac agttttttct tccgaggaaa catagccctc
     3481 ctccttattc ttttttagtt ctaccttttc tcgcgttttg acaagaagtc gaagtccaac
     3541 gaaccggagt ttcaatcttt cgattcgtac ttcgttcatg aatcggatgt tcctgcttcg
     3601 acaatttctt atttttagct acatttgaaa tgttacttct tttttctatc gtaggaaatt
     3661 gttctgttcg accaatcatt gcacttttcc gtgtttgtaa tttaccagaa gtcacttgtg
     3721 tttttcctgt ttgatgcgcc attgaacgca tcgcttgttg ttttctctct tgttgctcta
     3781 attgcaatgc atcttttcgt gcttcataaa aattcttctg tcgctcattc gcaaggttac
     3841 gcgctcgtgt ttgtcgttcc gactcattca ttcgcccaga gaaataatcg tatttcattt
     3901 tttgtgcttt gtattgggca ttggttggga catactgaat tgtccgtttc cattgttctg
     3961 ccctactttc aatcgttgcc ggtaattctg tcatagctga aacttttttt cctactcgct
     4021 ctataagagg tgtagtagtt atacttcgtt gaccactcat agatttggaa atcatttggt
     4081 cagctacacc tccggcagca ccaccaacga aattttcacc aattcctcgt atcatttttt
     4141 gacccattgt acctaacatc atgccacgta aaaaacgtcc cgttcgatcc ccagctcgtg
     4201 tttgtgcgcc accaattttc atccaaccta ataattcatt gacacgtgtc gagactgaaa
     4261 accataacgt cacctgtaag aaactggtcc aaatcattcc tttgttcttg gataagtggt
     4321 ttaactcgtg actaatagaa aaagtaatgg ttaaaatgag taaaatgccc atttttgtaa
     4381 acaataattg aaagactttc tgtaaggcat tacctaataa cgatcctgaa tttggaaaca
     4441 acgagaaaat catcgctaca ggtaaaaatc ctataaataa caagaacaat acttgcgacg
     4501 caattaagat gacgactaat gccaatacac ccatactaat gacaatgttg acaatgagcg
     4561 ttaaaaacac cataccaaaa cgcaaataca ctttttgtac cgaaagattg ccattttttt
     4621 gtttggttac ttcttcttca acaagcttat ttcgctgctc tccttcatca taaggagatt
     4681 cttttaataa attgattaca cgttcttcac caactgtttt tatatcgcta tccccaaatt
     4741 gcaatactaa ccagggttgc ttgatttgaa tggcaaatag attttcacga atggctttaa
     4801 ttgcatctgg ttttcctttt tgttcaggta acagatcttt ggcaatggtt aacatttcac
     4861 tattgatttc tttttgtacc tcattggctt tctctaaata tgtgtgcgta ttcataaaga
     4921 aaatgccgca acagataaac gtaaataaaa atgtcactgc ttgttgaatc gcttgcgtag
     4981 ggtgttgttt cactatccct tgataaacta ctgaacaacc tgctaaagca attaacaatc
     5041 caccaaagaa agggactaat ccatgactca atgtccctgc acggttcatt ccagcaatat
     5101 tctgaattga atgacttaac gaaccaatca actctgaaag aaaatctaat tcaaaggctt
     5161 cttggacaag aaaccctaaa aagtaacaca gatagacatt taacatccat aaaatattgg
     5221 taacggcaaa tatcccataa ataactgtat tagaaattcc ttcaccccat ttccacggca
     5281 accaattcca cgacgtatcc acaaaaaaat ctaaattata attatctaaa ggatatgttt
     5341 caaaatcata tacggcattt ttgccgccta gggtttcttt tatccaacca gctgcttcta
     5401 cgaatattgg gtgaaagaaa aaaacaccca tcattatgac tagacttatg aaaaccagtt
     5461 ttcggcgagt aattgaaaat gaaacctttg aataccactg tttcatctaa ttcacctcaa
     5521 tttcttcaac cagtttttct aatatctcgt ctttatattc ttcctcttcg tagttttttt
     5581 ccattggcgg tcgtgtatca aatgcatgga ataaatcttc aaaccaataa tacacataca
     5641 aaatgccaat attaccatgg atgtcacaaa acagacattc tccattttct agatttttta
     5701 cacgctggat attttcttct gtgacagaga ttccacaaaa gtttaatgct ttggtaattt
     5761 cttcgcgatc attcgaacga aaaatgaatt tcaacccaat tttacttctc atcttttcat
     5821 cgcctaaatc atctgtgttt tgtgttgaaa aatcaatccc tgcattgcga gatcttcctt
     5881 cacgaaccaa tttatttcct aaaatttttc cttgcgcaac attcaaccac gcccaacttt
     5941 catctaagcc aaccgcttta taaatatctc gattttgttt aataaaatct agcgcatatg
     6001 tagctaaact catcataatt gctatactta aaatttcaga agagttatag tcctctggtg
     6061 cagtttcact atctggtaac gttaaatcct gaaccagtgc gagattacat ttggcatcca
     6121 aatttaaccc ttgttgattc gtgccatctc caaataatag gcctgcaata gatagttctt
     6181 tgaagctttc aatatgcttt gccaggcgtt gtgcgatcgc tgcttcttgt gcatataatt
     6241 cctgaatcac tagaagtaat ccttttgttc cctcaaaatc agaaacagtt tttactgccc
     6301 gttgtaacaa aggaaaccgg tcttcatccc gaatacttac acccgtgata taggtgagca
     6361 catccaaggc taaactttct tgatcttttt ttcttctcat caattgaaat ggatcaagta
     6421 aaccaatatt ttctttctca ctggtaatat taactgtttt taagtagggg gcaaaatatg
     6481 gcaaatcttc ggaccaattt gaacgttccg attttggatc gatacaaaaa gccaaggcgc
     6541 caaacaacac cgaccaaaat aacaagagat tttcagttaa ggatttccca ccacctagcg
     6601 aaccaattaa tgctttggca agagtattgg taaccgttcc ttttattcct tgtgccgcta
     6661 accatggttg aataaacact gggattcctg tttctgcatt aaatccaatc ggaataccac
     6721 agccatctcc tagttgttgt gtcgcaccaa tgccaagact agcaataaat tctgcgccta
     6781 catattgacg ataatcctca atcactcgcc ttcctgctgg ataacttcca aaatgtaaaa
     6841 acagctgtac attgcacggt acttctaaat acatagaata gacatcaaaa aaattgcgaa
     6901 catcactcac acgttttaac atttcatcat aggtcttccc tgacacgcga acgtttagtg
     6961 ataatacata caaatcttct cgcgtatctt ctaatttttg ttccaactca ttggctgatt
     7021 ggtacgcttc gcgtacattg tttcctacat cttttccaac cgacatacca ctttcataaa
     7081 tatcagataa atcactcttt ttgccacgta ctttcgccaa ggccaaacga ttgtctaaaa
     7141 gattcacatc taaccaagaa tcgattggaa aatccaattc cgattgtaaa aaataataaa
     7201 tctctgattg aaatggaaat accaaatcac gggtcatacg tgaaagagat aaatacgtga
     7261 tatattcttt ttctgtccct tgttctaaaa ttagataatt tttcttttct gtgattcctg
     7321 cactggccaa attcaataca tcatagtgat acgtatacgt attcgtttct tctttaaagc
     7381 tattgggtcg atacatcact tcatctaagg attgttcttt ttttccttct aaatgttcca
     7441 ctaaaaacac catgtcttct ggtgttgttt ttcgtaaccg aaagtattta ctaatccggt
     7501 tatacaataa attctccaat cgcttatatc gttgaatttt ttcatttggg atggtttcaa
     7561 agtctccaaa caaacgggca ttggatcggt gaaaaaaatc ttttgtcgca atacgaaatt
     7621 ctttcatcca atgagcttct ttgggagcac tttcttctaa taccagctca atcccaacat
     7681 accaacaaac attttgaata tattttccat gtagctcttc taaaatgtgc tgaacacctt
     7741 ctaaatgttg gtaggcaatc gtttttaatt ccccttcaga acgcaccaat tcttttgagg
     7801 ctttaatcgt tttttcaatt ttttcttcgt ttgtacaaaa aaagaaccga acatgcgaag
     7861 aataactttg acgaatcaga cgcatgattg cttgttggag ttgatacgcc tgatcttctc
     7921 ccacgaagct atatgaatac attgggaatt cataataagc gtacacacga tcttcttctc
     7981 caaaaactaa attgtcttct aaataataaa aaggaatctt aggcactagt tttggtagtc
     8041 gcatcctttt cacctccata acgtactaca caacgaattc ctcgtttgat ttcttttttt
     8101 ctattggctt tacccattgg ccatgaaagg attcttttga acgacactga aataaaagaa
     8161 aagaagccaa ataattataa ggcttccttc cttgaaattg ttttcgtgtc attaaaaaag
     8221 caaataacca cggacaaaaa aattgtcgag taatcgtatg ttccataaca aatggaaaaa
     8281 ttcgaaaaat ccccccaaaa aagccaatcc caaaccatat cgcaaatgtt tgtgttacgg
     8341 gacgaggtaa cgcgacccca tcccacaatt tataaaacac tcgctctata taccaaatct
     8401 ttgtccaatt gcgtattttg ttcattttat cgcccccatg accgcatacg atccaaaatc
     8461 caattgaata cgccagacgt atctccaact aaccaaacag ctactgctag cacaagaatc
     8521 aatggaatgg ctttgcttac tttccgttca aaggcaatat atgccgcacc accaagcatc
     8581 gccaacataa tcaaatactt aaaatcccca acaaaatcag taaacacatt ttgaaaaaaa
     8641 ttcatttgct ttttcctccc tgttaatact cattgaacta atgacgatac acaataccac
     8701 agagcaaaac tgataaaaat ttcacttgat attcctcatt atgcattttt tccgttcctt
     8761 gattgctgtt ttttacgtaa gaagtataaa taatttctca cacgacgata cattactttt
     8821 acaggaacat gcgtacaata aatccggtgc tgcgtacttt tctttgtttt aggatcaagt
     8881 ttcatagaat agaaatcata aaaatactta ccactttttt tatcgtatgt tccttcaatc
     8941 accagtgaaa agctagggtg gtaaatctaa taaatacaat tgatacttcc ctctactaga
     9001 taaagggata aaaccaagta cctttaaatg atctgataca gtcataaaca cgatctatct
     9061 cctcccaaat aaaacaaaaa aaggctgtaa tcgcattttg attacagtct tttttgttct
     9121 ctttttataa atgtactaat taaaggttta cttccggtct ttttatttaa taaatcaatt
     9181 ttcccaaatt attttcttta gctttattaa cgatttctgt cgcaactacc acatctaata
     9241 cagccgaacc aactgttttg aagattgtga tttgttctcc acttgtccga ccgatcaatt
     9301 cttcattgat cagttggcct agttccccat gataactgtc tctttgaata tacccatctt
     9361 gcagcggtga aatgaagtcc ccagcttctt ttagaacacc gtccatggta tcaaaaataa
     9421 caacatctgc ggctttgatt atttctcgtg gaatttcaca catttccggt gtgtaagcac
     9481 cgactccgtt gatgtgcgca ccttttttga cccactcagc agagaatgtc gcacgtttgg
     9541 aagtggtcac acttgtaatg atatctgctc cttcaacgca ttcttgactg gtttttgttg
     9601 ggcgcatcgt tacagaaaac tgttgcatca tttcttcagc aaatcggctg gcacggtcaa
     9661 aatcaatatc aaaaatccga acttcttcta atttacgaac ggtcaacatc gcttctaatt
     9721 gcgactgtgc ttgtccgccg gttccgatca gtgcgccgat tttagcatct tctcttgcaa
     9781 gaagttctgt tgctgcacct tgaacagcgc cggttcttaa ttgtgtcaaa taggtaccat
     9841 ccaaacaagc ggataccata cccgtttctg gatcaaggac gatcatcgtt gctggtacac
     9901 ttggtaagtt tttcttgatg ttttcaggat acacggaaac gattttgaca cctaacgcac
     9961 cttcgccacc ttctacataa gctggcatat ataagctttg tccgttgctt tttggcacat
    10021 cgatattggt gcgaagaggc acacttgcct ttcctgctga atacaatgac aatgcttttt
    10081 tgtctgcatc gatggcttct ctcattgaga aacttttgat aatatcttct tttttaagaa
    10141 ataacatttg aatcgctcct taattagctg ctgaaacttt ttttaattga ctgcccaaca
    10201 ttgtatatgc actgtatagt aagaccaaaa ttacgatcca tttcaagaca taggtatcta
    10261 aagatttgac taggaataca gcgaccaata cccccaatac accgaaggtt gaagtgaaaa
    10321 gtgtgatttt tctgctgtat tcattaaatt taatgaattg aacactaccg atcggtactg
    10381 agaatgtaca agcacccatc atgataggaa aggctgccac tggattcaag cctagcaagt
    10441 agacagttac catcgttaac gcataagaac caatgccgat attgttcaat gcgccataga
    10501 caaagagtaa aaatcctgcc aagattaatt ttccgccata caattcactg gctgttccgt
    10561 ttgatggaat ccagttcatt tgtcctgcaa aaatcaataa tgcagcaatg accaatccta
    10621 tgccgacaaa cactttgatc gttttctctg gtaacttcac gacaaatctt ggtcctaaat
    10681 atgcaccgat aatttgacag ataatacaaa cagctagtgt cttgatccct acgttgatgg
    10741 cagtgatata agacaatgcc atgacggcta ccggtacgac acattgggta ttcaacgttc
    10801 cgggtagttt tttcatgctg acccatttca actttggata aagaacggtt ccgatagcaa
    10861 agtcggaaac gccaaatgtt gaaagaaaga agatgataaa tgacgtgaag ggcaacagac
    10921 gcatatcccc aggttcttct ttgaaatttt gtttgttttt gaaaaggtct cttagaa
//
LOCUS       DAXWLG010000086        10950 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXWLG010000086 DAXWLG010000000
VERSION     DAXWLG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.087"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            4..2376
                     /locus_tag="ACGE4T_001751"
     CDS             4..2376
                     /locus_tag="ACGE4T_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFF8387025.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACGE4T_001752"
     CDS             2437..3897
                     /locus_tag="ACGE4T_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFF8387026.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACGE4T_001753"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACGE4T_001753"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8387027.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACGE4T_001754"
     CDS             6128..6277
                     /locus_tag="ACGE4T_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387028.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACGE4T_001755"
     CDS             6352..6945
                     /locus_tag="ACGE4T_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFF8387029.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACGE4T_001756"
     CDS             6958..7857
                     /locus_tag="ACGE4T_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFF8387030.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACGE4T_001757"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACGE4T_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8387031.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACGE4T_001758"
     CDS             8723..8983
                     /locus_tag="ACGE4T_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387032.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACGE4T_001759"
     CDS             9428..9634
                     /locus_tag="ACGE4T_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFF8387033.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACGE4T_001760"
     CDS             9655..9885
                     /locus_tag="ACGE4T_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387034.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACGE4T_001761"
     CDS             9901..10338
                     /locus_tag="ACGE4T_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387035.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..>10950
                     /locus_tag="ACGE4T_001762"
     CDS             10331..>10950
                     /locus_tag="ACGE4T_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387036.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDT"
BASE COUNT         4125 a         1825 c         2300 g         2700 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggga cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta
//
LOCUS       DAXWLG010000087        10874 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXWLG010000087 DAXWLG010000000
VERSION     DAXWLG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.088"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            54..1010
                     /locus_tag="ACGE4T_001763"
     CDS             54..1010
                     /locus_tag="ACGE4T_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8387037.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1083..1199
                     /locus_tag="ACGE4T_001764"
     CDS             1083..1199
                     /locus_tag="ACGE4T_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFF8387038.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1254..2936)
                     /gene="recN"
                     /locus_tag="ACGE4T_001765"
     CDS             complement(1254..2936)
                     /gene="recN"
                     /locus_tag="ACGE4T_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFF8387039.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2954..3403)
                     /locus_tag="ACGE4T_001766"
     CDS             complement(2954..3403)
                     /locus_tag="ACGE4T_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8387040.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3510..4328)
                     /locus_tag="ACGE4T_001767"
     CDS             complement(3510..4328)
                     /locus_tag="ACGE4T_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFF8387041.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVASNAHEQFDHK"
     gene            complement(4337..5227)
                     /locus_tag="ACGE4T_001768"
     CDS             complement(4337..5227)
                     /locus_tag="ACGE4T_001768"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8387042.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5229..5453)
                     /locus_tag="ACGE4T_001769"
     CDS             complement(5229..5453)
                     /locus_tag="ACGE4T_001769"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFF8387043.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5458..6795)
                     /gene="xseA"
                     /locus_tag="ACGE4T_001770"
     CDS             complement(5458..6795)
                     /gene="xseA"
                     /locus_tag="ACGE4T_001770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFF8387044.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6796..7650)
                     /locus_tag="ACGE4T_001771"
     CDS             complement(6796..7650)
                     /locus_tag="ACGE4T_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFF8387045.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7747..8196)
                     /gene="nusB"
                     /locus_tag="ACGE4T_001772"
     CDS             complement(7747..8196)
                     /gene="nusB"
                     /locus_tag="ACGE4T_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFF8387046.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8189..8614)
                     /locus_tag="ACGE4T_001773"
     CDS             complement(8189..8614)
                     /locus_tag="ACGE4T_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8387047.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8860..9924)
                     /locus_tag="ACGE4T_001774"
     CDS             complement(8860..9924)
                     /locus_tag="ACGE4T_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFF8387048.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10096..10341)
                     /locus_tag="ACGE4T_001775"
     CDS             complement(10096..10341)
                     /locus_tag="ACGE4T_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8387049.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10480..10854)
                     /locus_tag="ACGE4T_001776"
     CDS             complement(10480..10854)
                     /locus_tag="ACGE4T_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFF8387050.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
BASE COUNT         3063 a         2422 c         1779 g         3610 t
ORIGIN      
        1 attccaattc cctatccgtt ataataaaag aaacaggcaa caggaggaaa atcatgatca
       61 aatattttac attagataat gagaaactgc gtaataccgg aaaggaaact gaccaaacga
      121 tctggtattg tgtagaacgt cctaatcaag aagagattca tcagctgaca catcaatttg
      181 aaattccgtc agattacatc acttccatat tggacgatgc agaaaattcc cgtacagaag
      241 aattcaatca atcaaagttg acaaagccag cgttattatt gttacaatat ccttttgcta
      301 aaacaagtcc tagcggttat tttcaagtgg atacataccc gctctctatt attttgacgc
      361 ccaaaaagaa gcttatcaca attacgaatc atgaaccact gtttttaaag aaagtcttta
      421 gccagacctt tccaaaaagt gatatttcgc ctaatctcaa tatcttcttt caattcattt
      481 ggcaggtcac actctcttac aacagttatt tagctacgct tcaatctcaa tgcgatactt
      541 tggaagccaa acttcaagta tccactgaaa acaaacaact ttatcaaatc atggatatcc
      601 aaaaaagcct cgtttatttc agagaagcga cttctgctaa tttagatgca ttgaaagaac
      661 tagcgcaaaa taaacttttc caagaaaatc atgctttgaa aaatcattta cgtgatgtat
      721 tggtggaaac agagcaggca atgactactt ctcgcattca attaaaacta gtagaacaaa
      781 tgaatcagac tttttcagca atcgtatcga ataatctgaa taatattatg aaaattttaa
      841 cttctttaac gattatttta actataccga caatcgtcgg cagtatctat ggaatgaaca
      901 ttaagttacc tattgctgaa agggatgatg cctttatatg gctagttcta ttaacattat
      961 ttatttgtat cgtaactacc tattatttaa aaaaagggaa gtttttataa aaaagccctc
     1021 ttgtggaaat ggaaaaaaga ttgtacactt gaatcaacta attaatgacg aggttgaaaa
     1081 caatgcgtga aaaaaaacaa acaagtactt ttgctaaagt aacaaaattt gtcatttgga
     1141 caatgctgat tttaacgatt ggatctgttg tattgactgc tgtaatgagt gtcatgtaaa
     1201 aataaaatat agctatcagc tgctgaaaag gacctgaacc actgcgaggt ggctcaggtc
     1261 ctttttgcga gtttcaatag ttcttttgca tgttcgagtg tgagttttgt aatctcactt
     1321 ccagccagca tacgtgcaat ttcttcttct ctttcttttg ttttcaactc tgaaacagaa
     1381 gtttgcgtac gtccatcaat cactgctttc acaatataat attgatagtc ggcgactgca
     1441 gcaacttgag gcagatgagt gatgcacaaa acttgagagt atttcgatat ttgagaaatt
     1501 ttatctgcga tggcctgtgc aactcttccg ctcacacctg tatccacttc atcaaaaata
     1561 atacttgtca caccttgctc agaagaaaaa atagttttca aagcaagcaa catccgagac
     1621 agttcgccac cagaagcgac tttcaccaaa ggcttcagtg gctctcctgg attggtcgta
     1681 atgtaaaatt cgatttggtc aaatccatca tgattcaatt gtctattttc ttcttttaag
     1741 aaacgtacct caaactcagt gttttccata tacagacttt tcaattcatg cagaatggat
     1801 ttttccaatt cttttgcgat ttttttacgc gcttcatgaa gatctgcggc ttgttgctga
     1861 agtagaagct ctttttgctc tagttcctct ttcattttgt ccaattgacc ttctgaaaaa
     1921 tcagaagaag ctagctcttc ggtgatttcg tcatagtaag ccaatattga ctcaatggat
     1981 tcgccgtatt tacgtttcaa ctgtcggatt aattctaaac gttgcgtaac ttcttctaaa
     2041 cgactctcat ctaactccaa taaatcaatc tgacgactca tatcgccgat tgcatcttgc
     2101 agcaaatagt aagcactttg gatattttca taaattgctt catactccac atctagatgg
     2161 gcgatccctt gcagttctga aacggccaag ccaacaccat ctacactgtt ttgttcatta
     2221 ttcgtcagtg ctccgtaacc tgcagctaat ccatcagcaa ttttttgata attgctaagt
     2281 ttttctcttt cttcttttaa tttctcttct tctccgactt gcaaatcagc agcagctatt
     2341 tcttcttgct ggaaatggag catatctatt ctctgaacat aggatttttc attttgctgc
     2401 acttttcgta cccttttttc cagctcacga taagagatat attcttgctg ataactcttt
     2461 ttcttctgtt ggaaagctac atctccaaaa cgatccaata aagcaagatg tgattcaggc
     2521 tggagcagtt cctgatgttc gttttgtcct tggatatcta ctaaataact gccgattctt
     2581 ctcagattag ctaatgtgat aatatgacca ttcactcgac aaacattttt tccagttaag
     2641 gacatatccc gtcgaacaat cagattgtcc tcatctgttt cgatccctaa ttcaaccatc
     2701 agttcagaga atccttcttg ttttggtagt tcaaacaacc cttcaagcac acatttttct
     2761 gctccttgac gtatataatc gcttgaacct cttcctcctg ctaacagacc taaagcatcg
     2821 ataataatgg atttacccgc accagtctct ccggtcagcg cggtcattcc ttcgtgaaaa
     2881 gaaagccgca attccggaat aatcgcaaag ttagtaatac tgatttcttg tagcatcaca
     2941 ttcgctcctc aacttaaagg taatgcagga agtctttctt caataaaact gcctggtctt
     3001 cagttcgtgt aatcatcaaa acattatcgt catcattgat aatcgaaaat aattctttct
     3061 tgtaacgttt atcaatcaaa tttgctgctg ccgaagcatt tcccggcaat gttttcaaaa
     3121 taacaaattt ttccatttgg tcaactgcca cgaaagcgtc ttttaatagt ttttccaatt
     3181 tagtatttac atcttcgttt gtttcagctg gtatgctata acggtagccg ccatctgaag
     3241 ccggtacctt gatcagtttc agttccttta tatctctgga aatcgtagct tgagtaacag
     3301 atattccttt gcttttcagg atttcaacaa attcttcctg ctttcggata tcttgctcac
     3361 tcaacaatcg agtaatcaaa cgatgccgat ctttttttct cattacgtct tcgctcctta
     3421 gcatattctt cttaatcaat gatagcttat ttccttggta taataaagaa gaatccgcat
     3481 acttctcact tgattttcac agttacaatt tatttatggt caaactgttc gtgtgcattt
     3541 gaagccactt ctgccatatt aatagctgaa ttgattgttc cgctttcggg tactttcctc
     3601 aagtgagcca agaactcgat atttccttct ccccccgtga tcggagaata acttaattcc
     3661 agcacatcat atccaccttg tgccgcaaat gacaaaatgt cttctaaaac tttttgatgg
     3721 gtctgagggt cacgaacaat tccgtttttc cctacagctt ctctgcctgc ttcgaattgg
     3781 ggtttgatta aagcaatcac ttctccctct tcttttaaaa tagcatgtaa tggaggcagc
     3841 atcagtttta gtgaaataaa agaaacatcg ataacagcaa cttctggaat tcctaaggta
     3901 aagtcttcgg gttttgcata acgaaaattc actctttcca ttacgacgac tctaggatct
     3961 tgtcttattt tccaagctag ctgattgtag ccaacgtcta aagcataact catttttgcg
     4021 ccattttgca aagctgcatc agtaaatccg ccagtggatg caccaatatc taacaatgtt
     4081 tttccttcaa cagatagatc aaatgctttg agtgcttttt ctaattttaa tccccctcga
     4141 gaaacataag gaagtttttt acctttgact cttaattcac tactcactgg aattttttct
     4201 cccggtttat caaaccgctc gtttttttcg ttgtaaatca aaccagccat cactgaacgt
     4261 ttggcttgtt ctcttgtttc aaataatcct tgtttgaccg ctaatacatc tacgcgttct
     4321 ttttccattc tttccctcat ttctccacat caaattgctt gatcatctgg cgcatcagcc
     4381 cgccatcaaa agaaaaaacg tcctcttcta atttgtctat gatcttgttg gcggataaca
     4441 gctcaatctc caacgcctca cgtgtctttt ctaggcctaa taaacgcggg taagtatttt
     4501 tttccatacg ttcatcttta ccagctgttt tccctaatgt tttcgttgta ccaatcacat
     4561 cgagtagatc atctcttatc tgaaaagcta aacctaaatg ttcggctaat cgttgaagta
     4621 gcaataagat ttcttcaggt tgcttcgcta atatgccacc agccagcaag gcataacgaa
     4681 tcaaatgccc tgtctttctt tcatgaataa atgctaattc ttctaaagaa agctttttcg
     4741 attcgccctc gatatctgca gcttgcccag caaccatacc ttgtgatccc gcacaaacag
     4801 ctaattgctg aagcagcaga agcttaggag aattacctaa gtgtgccata ctgattagct
     4861 ggaatgctcc tgtaagcaaa ccatctccgg ccaatatagc taatgcttcg ccgaatactt
     4921 tatgattggt aggttttcct ctacgaagat catcattgtc catcgcaggc aaatcatcgt
     4981 ggatcaagga atacgtatgc accatttcta aagcagctgc tacttgatag acgggaacat
     5041 caataggctc atcaaaggaa gcaacggccg ttaaaagtaa taagggacgt attcttttcc
     5101 cgcctgcttt cactgaataa ctcatcgcat catagagtcc tctatccgtt gtatgctcat
     5161 ctaaaaaaga caagatttct ttttctacag caggtagatg aatagctgaa aagtcagtta
     5221 atctcatctc agctttcctc gtttccgtca aaatcaactt cttcatttga ttctgtcatc
     5281 attttcgtca aggtctcttc tgctttagct aatgtatctt ggcaatgttt gcttaattcc
     5341 attcctcgtt taaacgaatc tagagctgat tctaatggaa cgtctccttg ttctaactgc
     5401 atgacaattt tttctaattc ttgcagtgac tcttcaaaag ttggattttc catattttta
     5461 ttctcctttg tctatctctt gtacttgtcc tttaacgatt ccatcttgat aatggatcgt
     5521 cagcaaatcc ccttgatgaa tctcggtgac actttttact acttgatcat ttaacgtcgt
     5581 atagctgtag cctcttccca tgattttcaa tggactcaac aaatcaagag attgaacagc
     5641 ttgagcaaaa cgctgctgct gttgtttcat atattgttgg attttctctt ctaagcgttt
     5701 ttctagataa gcagtttctt ggcgagctgc tttaactcga taaagcggag ccacttgttc
     5761 caattggtgg cttaatgcga tcgtttgttt ttctttcgca tgataaattt gctgtgtagc
     5821 ttgatataat cgctgtttca actggtctaa tttgatggtc tgaccttcat acaatctttc
     5881 aggctgccga aatacatagg aacgtcgagc acgctcgaac cgttcttgtt tgcgctggat
     5941 ctggcgtaca aacgcctgct gcaatctagc ttgccgttct tgaatgcgca tcaactcttc
     6001 tgacaaaaca ggaacagcta attctgcagc tgctgttgga gtggcagccc gaacatctgc
     6061 tactaaatca gcgatcgtgg tatctgtttc atgtcctaca gatgaaatca ccggcgtatt
     6121 agcttgataa atcgcccgag caacccgctc ttcattaaac ggccataaat cctcaatcga
     6181 accaccccca cgaccgatga tcattgtatc gaaattgccc atctcttcta ctctctggat
     6241 attcctgaca acattgtccg ctgcttggtt tccttgtaca actgttggga acaaaaccaa
     6301 ttggacaatt ggatatcttc ttttcaccgt cgtgatgata tcgcggataa ccgctccgct
     6361 tggactggta aggaccgcga ttcgctttgg gaaacgtggc agcatttttt taggcccttg
     6421 aaaaaggcct tccttcatca gcttttcttt tcgttcttcc aattcttgat ataaagcacc
     6481 tattccatca ggctccatgt gctctacata gatttgataa gaacctcccg cttcatataa
     6541 tgaaatacgc ccaacgacaa ggactttcat tccttctttt ggctggaatc gtaatttttg
     6601 gaatgctcct ttgaacataa tagcagatat ttttgcatgg tcatctttta agctgaaata
     6661 ttgatgggca ttggcacgta aacggaaatt cgatatttcg cctgttaaat atacgcgttc
     6721 taaataagga tcggcatcaa attttctttt caaatacttc gttaatgtag tgacagttaa
     6781 atattcttgt gccatctatt cacctgctcc aaaagcttcg gcagatttta cggtttgata
     6841 catcagcatc gtgatagtca ttggccctac accctttggc acaggagtga tccagcctgc
     6901 aataggctcc acttcatcaa atttcacatc accgatcagc ttaccgttag catcacgatt
     6961 catccctaca tctatcacta cagcacctgg ttttacgaat tcttttgtga caaaatgtcc
     7021 acgtcctatt gctacgacta aaatatctgc ttcttgtgcc agttcagcca gattttcggt
     7081 tttagaatga gcaacagtaa cagttgcatc tgccatcaag agtagctgag ccatcggttt
     7141 accgacgata ttgcttcgtc cgataactac tgctcgttta cccgccggat cgatagaata
     7201 agcttcaaac attttcatga tgccgtatgg agtacaagga atcttgtccg gtttacccgc
     7261 aaggagtctg cctaaattca tcggatgaaa accgtctacg tctttttcag ggcggatagc
     7321 taacaggatc ttttcttcat ctatatgctt tggcaatggt aattggacca atataccatg
     7381 aaaactagga tcttgattgt atttttcaat ttctgcaagt agttcgtctt cagaaatcgt
     7441 ctcaggataa cgctctacca atgaatggat tccgatttct tttgctacac gttctttatt
     7501 tcttacatag atttggctgg ctggattttc ccctaccaat aaaacgacta atccgggatg
     7561 gataccatta tctttcaatt tttggatttt ttcagcaatc tctgcctgca ttttttcagc
     7621 taattctttt ccgttaatca attctgccat ctaaccactc ccagatattt tttgtgtttt
     7681 cattctacca tagaattttc ccgatttata acaggtttat gatgattcat agaaaaaagc
     7741 taggacttac gccccagctt ctaattcttt caatacattg gataaaacac cgttgacgaa
     7801 tttacgtgaa cggtcatcac taaatgtttt tgccaactcg attgcttcat ttaaagcaac
     7861 agccgctggt acatctgtca cataagtcat ttcaaaaatc gcgatacgca agatcaccaa
     7921 atccattttt gagatccgat tgatgtgcca attgtttttt aaatgttttt caatcacttt
     7981 atccaattca tctttttttg cacagacacc gccaactagc gtatcaagat aaacaggaac
     8041 aaactctgat tcatcttcgt tgatcatgtc gcggtggtct aattcgattg cgttgaaaat
     8101 cgcatcttct tttgttaggt ctgcattgaa gtctaatgga aataaagcct gcaatgccat
     8161 ttcccgaatc tcgtgtcttg ataattcttt attcattctc ttcctcttcg tcttcaaaaa
     8221 gttcttcgat tgttggttgt tctaattttt caggaacaac agctactaca tggatattga
     8281 cttcagataa atcgatatcc gtcatgaaaa ggacttgctg gctgacccgt tcctgcattt
     8341 ccattgctac tttaggaact gaaacaccat attctagata acagtaaaga tctactttca
     8401 agccatcttc ctcgtttgct aagtaaacac cttttccgtg agctgcacgt cctaataatt
     8461 cagtgacatt gctggcaaaa gttccgcgca ttccgtatac gccttctact ttagaagctg
     8521 caatcccgat aattacttcg atgacttcag gagcgatcac gatctctcct aaatcttggt
     8581 ttgcgtttaa aatcaaattt ttttcatctg ccatttattt attcctccct ttgcctaagc
     8641 acgagataca tccgaattct ctatgatgcg gcatagtgat cacgtggtaa gaaaactgcc
     8701 gccgatcagc tgcaaactgc tatgaattgg agaaaacacc caactttatt atctcttaca
     8761 gcttaaaagt tcttctatag tgtatcatta aatcgagtga ttttctataa caaagtctgt
     8821 gaacactgat ttttacgctt acttcttcta ttgatgaaac tataagatga tcaattcttt
     8881 tggtgaacga gtcaacacac gatttccatc ttctgtgatc aacaagtcat cctcaatacg
     8941 tactccaccg attcctggta agtagatccc tggttcatca gtgatcacat ttcctggtac
     9001 aaattgcttt tctgcacgag aagaaacgtt tggtccttcg tggatctcta aaccgatccc
     9061 atgtcctgta gaatgtccaa atgcttcccc gtaaccatgt tttgaaatat aatctctagc
     9121 aactgcatct aattggacac cagtcattcc tggttttgca gcatcaataa ctgctaattg
     9181 agcatctaag actatttgat aaatttcttt taatttttcc ccaggatcac caatagcgaa
     9241 tgtacgtgtc atatcggaaa cgtacccttc gtaatagcaa ccaaagtcca atgtgatcaa
     9301 gtcaccttgt tcgatgattt ttttgcttgc tacaccatgc ggcatagccg aacgcaatcc
     9361 gctagcaacg atcgtatcaa aagaaacacc tgaagcgcca agagaacgca tataaaagtc
     9421 tagttgattt gcgacttcaa tctcagtcat gcccggttgg atcattttca ggatgtgatc
     9481 atatgccata tccgcaatat gacatgcttt ttcgataata gcgatttctt cttcatcttt
     9541 cacttctctg agttcttcga tcatctgtga aacaggtact aattccgcat cgatgatttc
     9601 ttctaaaacc gaatattcta aaaatgagac tgttgtttcc tcaaaagcta atgagtgcag
     9661 ttcttctttg cgtactaagt ttgctacctc atcaaaaata ggtccgacat ttttaataat
     9721 ttcaaaccct tgagcctgcg ctgccgcctg ctcggtataa cgaaaatcag taataaaaaa
     9781 tgctttttcc aaagtaataa cagctaatcc tgtcgtacct gtaaagttcg ttaagtaacg
     9841 aagattgtac ggactggtaa ccaaaaatga atccaaattt tcttcttgca tttttttgcg
     9901 caatttttct acacgtagca tcataatgaa actccctctt tctttgcacg cttttttcca
     9961 ttatagcaaa cttttcttcg tacacgacag ttaaataaaa atttccggaa aacttctttt
    10021 tattttaaat aaataaagcc aaaaaggagt cacagtctcg ctgcaactcc ttttagttta
    10081 ctattttgta aaaacttatg atcgttttcc aaagaaaaat gagacaactg ctaccaaaat
    10141 cactgcgcct aaaatagatg gaataagtgc catacctgca gcttttggtc cccattcgcc
    10201 gaaaatcgct tgtccaatac ttgaaccgac tagtccagca atgatattcg ttacacagcc
    10261 catcgaattt cctttactgg tgatcgcacc tgcaattgct ccgatcacgg ctccaacaat
    10321 caatgtccat aaccaactca taatataacc tccttctttg ctaatcaatt ttagcatatg
    10381 ggatttaaag gggacaaata attggttttg ctatttcata ttttcttctg caaagtaaaa
    10441 aaataaagaa aacctcctct tagaagtttt ctttattttt tacttgcgtt taaacatact
    10501 acgaatacca cgtttcacta catcaaagaa actcaatgat tgtaccattc tatcttcatc
    10561 tacatattct ctgatcgcac gaagcacttt tttaggttct ttggaagaaa atgtgtatgt
    10621 accatttttt ttcgtttgaa tggcatatcg aggaatccat tttcccttta gcatgactga
    10681 tgctagtaca taatccactt cttcccatgg aatttggata tatttgcgtc tatctcgcgc
    10741 atggtaaaac tcaaaaccat tatcacctat catgatttgc ccgtaatctg tcagacccgt
    10801 aaacgaagta gcatcaatta ccaagtctac ttttgtattg attgattgaa ccattttctc
    10861 cctccagata tttg
//
LOCUS       DAXWLG010000088        10690 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXWLG010000088 DAXWLG010000000
VERSION     DAXWLG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.089"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(79..2199)
                     /locus_tag="ACGE4T_001777"
     CDS             complement(79..2199)
                     /locus_tag="ACGE4T_001777"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8387051.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACGE4T_001778"
     CDS             2429..3607
                     /locus_tag="ACGE4T_001778"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFF8387052.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACGE4T_001779"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACGE4T_001779"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFF8387053.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACGE4T_001780"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACGE4T_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFF8387054.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACGE4T_001781"
     CDS             complement(4960..6213)
                     /locus_tag="ACGE4T_001781"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8387055.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACGE4T_001782"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACGE4T_001782"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFF8387056.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACGE4T_001783"
     CDS             7790..9136
                     /locus_tag="ACGE4T_001783"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFF8387057.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACGE4T_001784"
     CDS             complement(9444..9830)
                     /locus_tag="ACGE4T_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8387058.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACGE4T_001785"
     CDS             complement(10098..10247)
                     /locus_tag="ACGE4T_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387059.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3457 a         1914 c         2127 g         3192 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata
//
LOCUS       DAXWLG010000089        10655 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXWLG010000089 DAXWLG010000000
VERSION     DAXWLG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.090"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..570
                     /locus_tag="ACGE4T_001786"
     CDS             <1..570
                     /locus_tag="ACGE4T_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFF8387060.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            574..1215
                     /locus_tag="ACGE4T_001787"
     CDS             574..1215
                     /locus_tag="ACGE4T_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8387061.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1212..2129
                     /locus_tag="ACGE4T_001788"
     CDS             1212..2129
                     /locus_tag="ACGE4T_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8387062.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2126..2797
                     /locus_tag="ACGE4T_001789"
     CDS             2126..2797
                     /locus_tag="ACGE4T_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8387063.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3338..3826)
                     /locus_tag="ACGE4T_001790"
     CDS             complement(3338..3826)
                     /locus_tag="ACGE4T_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFF8387064.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4027..4293)
                     /locus_tag="ACGE4T_001791"
     CDS             complement(4027..4293)
                     /locus_tag="ACGE4T_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8387065.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4293..4556)
                     /locus_tag="ACGE4T_001792"
     CDS             complement(4293..4556)
                     /locus_tag="ACGE4T_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8387066.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5077..5217
                     /locus_tag="ACGE4T_001793"
                     /pseudo
     CDS             5077..5217
                     /locus_tag="ACGE4T_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5359..7182)
                     /locus_tag="ACGE4T_001794"
     CDS             complement(5359..7182)
                     /locus_tag="ACGE4T_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8387067.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7780..10485)
                     /locus_tag="ACGE4T_001795"
     CDS             complement(7780..10485)
                     /locus_tag="ACGE4T_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFF8387068.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3054 a         2218 c         2021 g         3362 t
ORIGIN      
        1 gcaagccgta tcgccatcat gagtgaaggc cgtgtgatcc aatttgatac accagaaaat
       61 attttacgtc atcctgcaaa cgatttcgta gaagagttga ttggagaaga tcgcctacta
      121 caagccaagc caaatgcgac aagagtcggc gaagttatgc taaatacagc aatcacgatt
      181 acaccggaaa aatcattaca agaagcaatc aaactgatga gggaaaaacg tgtagatacg
      241 ttgttagtta ccgataattc gaatgtatta aaaggattta tcgatgtaga aacgttgaat
      301 aaaaaacgag gaaaagtatc cagtgtcggc gacattttga ataaacatgt tttttacgta
      361 aaagaatccg cctatcttcg tgataccttg caacgcattt taaaacgcgg gctgaaatac
      421 gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg
      481 gatatcgtct acgatgtgat ttggggagaa gatcccgcaa ctactgatgt tgcagagtca
      541 gctactcctg aagaatctaa ggaggtctga cctatgcagc agttttttca tcagcacggt
      601 tcagaaattc tctcaaaagg ttttgaacat ctttatattt caggacttgc tttgatcatt
      661 ggtatcatca ttgctgtacc gcttggtgtc ttactgacac gttttccaaa agctgccacg
      721 attgtgattg gcttaaccag tgccttgcag acaatccctt ctcttgcttt attggcattg
      781 atgatcccgc tatttggcgt aggtaaatta cctgcgatta tcgcactctt tatttattca
      841 ttattgccga ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg gaattatcgg
      901 gatgtggcaa aaggcatggg gatgactgag ttccaatcca ttttttccgt tgaattgccc
      961 attgctatgc caacgatcat ggctggtatt cgtttagctg ccgtttacgt gattgcatgg
     1021 gctacacttg cctcttatat cggcgctggt ggtttaggcg atttgatttt tagtggactg
     1081 aataattacc aaccagatct cattttggca ggtacgatcc ctgtaacaat tttagcacta
     1141 ttggctgatt ggcttctagg tctcttggaa caccggctga caccgatcgc acttagagag
     1201 gagaatgaag aatgaaacgt atactaaaaa tgaccatggt tcttttctct cttcttatac
     1261 ttgcgagttg ttccttccct ggtcttgcca gcaatacaga tgaagacacc atttcgatca
     1321 caggtggaat cacttcagaa gcacagatct tagcaagtct agttgccgga atggttgaac
     1381 attatacaga taaaaatacc gcaatcatca acaatctggc tactacaact atcaaccatc
     1441 aagcgatgat gaacggagat gcttcaattt cagcggctcg ctacacagga acagatttaa
     1501 cgactacatt gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag
     1561 atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc ctatggtttt gaaaatacgt
     1621 atgtctttct tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa gtcagcgatc
     1681 tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac ttcttggatc aatcgtaaag
     1741 gagacggtta tgacggcttc caagaaactt atggcttctc cttcaattcg atattaccta
     1801 tgcagattgg acttgtttat gacgcagtcg aagcaggaaa aatggatatt gttcttggct
     1861 actctacgga tggacggatc gccagttatg atctagtcat gttggaagat gatcgtcaat
     1921 tcttcccacc atatgatgct gctccagttg tagacaacaa gatattagct gatacgcctc
     1981 atttaaaaga agccttaaat aagttaggtc ataccatcag tacggaaaaa atgcaggaat
     2041 tgaactacga agcagataat aatctagtgg aaccatcagt agtagcacaa cgtttcttac
     2101 aagaaaacca ctattttgaa gggaagtgaa gcaattggat aaaatgaatg tctttcaaca
     2161 atttttctat tatttccagg aaaatggctc ctatatcttt gcacaattta tccgtcactt
     2221 cctgatttct atctatggtg tcctctttgc agcagttgtc gggattccgg ttgggatcat
     2281 gatttctagg agacgtaaat tagcaaactg ggtgattcgt ttggcgaata tcatccagac
     2341 gatcccttct ctagccatga tctccatttt aatcattgga ttaggcctgg gagtcaatgt
     2401 cgtgattgtt actgtatttt tgtattccct cttaccgatc atcaagaata cgtataccgg
     2461 gatgatccaa gtagacaaaa acatccttga tgtcggcaaa ggcatgggga tgactgctag
     2521 acaacgacta ttcatggtag agttaccact ctctgtttct gttatcatgg caggcataag
     2581 aaacgcgtta gttgttgcca tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg
     2641 tcttggagat atcattatcc gaggaaccaa tgcaacagag ggcacatcga ttattttagc
     2701 aggtgcattg ccaactgcat taatggcgat catcacggac tggcttttag gtattttgga
     2761 aagacggcta gatccagcca gccgtacatc acgctgatgt tattgtaaaa aacaaaagct
     2821 tctgcgacca tttgttggtc gcaaaggctt ttttagttct gtcattttta aggcaataat
     2881 ccactctgat tcttccctcc ttatgggcaa agagttgctt ttcttagcta tttatccatt
     2941 ccgtatactc caacttcata ctgccaacgg gttcatatct gcatccaccg actaagtttt
     3001 tttcttatcc gctaaggcat tttgccaatc cattttccca tcactcgtca tggaattcat
     3061 accacatgat ctctaaatac tcgtctgctt gttctttgaa ttgggcatca attcttacat
     3121 cctttggcgt ggctttcatt taagcctttt ccatcagtcc tcgattgttc gatgccttca
     3181 gctgcatcat caaaactggt gactgccgta tcggtaactt cgatcatctt ttatacaatc
     3241 gggaacttgc catcctcatc cttggctaaa aacatatcta gccattccag cttctcgcca
     3301 tgggaggttt gcggttccta aaaccggtac acgattctta tggactgcat ccgtcgcaac
     3361 tagacttggc gatacgatga tccttcacca gaagagcacc cccgtaaacc aattgatcga
     3421 tatattgcca ataagaaaag acattggcgt aaaagatatc gatccctcgt gatgcattgc
     3481 cgttgatact gctattcatg gccgaaagcg ccacgacttt aacttcttcc gtttgagtga
     3541 cattcatgtt cggcaatgtc gctttggata cccgctctgc taaaggaacg acactggtat
     3601 tgtatttcgt atctgtatct tttccaaaag accatgccaa cgaatcctct gggaaccaat
     3661 aagacgactc cggttgattg tccattgctt ttttcaacat acttttggct gtttctcgat
     3721 aaacgacatt tccaattgtc ttcgcctcac tttggcgata atcgatggtc gttgccactg
     3781 ccaacactaa caacgatgct ccaattttac tgattttcct tgtcacactt cttcctcctt
     3841 gttttacata aacccgtcaa cattccattc ctaaactcaa acaatgaccc ttcaaaggtg
     3901 gagatatagg aacaaattaa agaaatcatg caataaatcc aagaaggttg gttttagaac
     3961 aaaaaaagag tctccttttt ggggagactc tttgttaact atctaaaaag gttggctctt
     4021 ctcacttcac tcataatgtg tccaggcaga ataaatttct acaactttat tttcctcatc
     4081 aactttgtaa accactcgat gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt
     4141 aggtctcagc ttttcaaaag actgcgtggg catatatgga tcttccttca atgtctgaat
     4201 cacttcttca aattgtttct ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc
     4261 agagtttttg attgcgactg tatagttact catcaaagag cttcccaatc aatatcatcg
     4321 atattcgtaa agctactgtc gtctttttcg cggtctcgta ctttatctaa agtacctgtt
     4381 tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt ccagcccaat caatacagca
     4441 ttgtttccac tacgatcact aataatctcg atttcagtat gattttcatt tacctctttc
     4501 aataactgat aaaaattttt acgagcagtg ctaggattca tcgttgtatt agccattgtc
     4561 tctcctcctt tttctgatat aattatagcg tacttgatta agtacgtcaa aaaaatacaa
     4621 taaagcactt aaattcagca aacttttcct cttttttaca tagagaaaaa gacccctttt
     4681 tagggaggat ctttgtccac gctcacttct tactccttta cgactagcgg tagccaaacg
     4741 taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc
     4801 tatgcaacaa caccaataca cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg
     4861 aataacctga ggatacgcac gaaagatttg taacggttat tcaaaactga ttttggccgt
     4921 caagtcttct actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt catgttcact
     4981 atcgatttga tacttgctta gaccgttggg tttgtgaatc accaattaaa aatggcactg
     5041 agttatggta acaaaaaatg gtcacttgac gaggctgaga ttcctactta tgaagacaca
     5101 tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata taggaggctg tacattattt
     5161 ggcaaaggat tcggaaatta tgatcaagac atcaaaatca aaatgtttta cagctaaata
     5221 ggaagaataa taacatttca tacgctgttc ccatctttaa tggcttatag agggcacctt
     5281 tagctgagaa taagacaaaa cagaccctct agcttcaatt aagagctaga gggtctgttt
     5341 ttgattttaa aacttggttt atttcttcaa atggtagcta taggtgctga caacgatatt
     5401 ttcacgccaa gacaatcgca atcttaaccg caaacttgtt tggttatgga gaatattctg
     5461 ccagcgtctg tttggtacaa attgaggaat caacacagta gttgtatagt tttgtttcgc
     5521 tgcattttta cttacgatat ctacataacg gataattgga ttttcaatcg agcgataaga
     5581 agagtggacg attgaaaaac ggacatctgg gaaatgcttt ttaaattctg cttcgatttc
     5641 tttttctttt tcgacatctt cgtctagcga aacatgcatc gcaacgacat aatcaccgat
     5701 cgaacgtgcg taattcaacg caccgatatt tacccgtgtc acattcccga ctaatacaag
     5761 aacggtattc ccgtcataat catgtagttt tgcatcattt tctaaacgaa gctgttctgc
     5821 tactttttta taatgatcat ggatcttata aaaaatgaac ataacgattg gcataatgat
     5881 gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa atcgcaatga ttgcatatga
     5941 gatgaacgca cccgtaatat tggcaatcga tttgcttaac catctttttc cttctttttt
     6001 ccatttgacg accatccctg tttgagataa agcaaatgga atgaacacgc cgatagagta
     6061 taacggaatc aagcgttctg ttgagccatg gaaaataaac agcaatacga tcgaacctag
     6121 cgctagtgtg ataatcccat tggaatagcc tagtcgatcc ccacgatctt gatacatgtg
     6181 aggcatgaat ttatcttttg ccagattgta tgcaagaact ggaaaagcag aaaaaccggt
     6241 gttagcagca actgctaaaa tcagcgctgt agcaaattga aggacgtaat acaaaatacc
     6301 atgtccaaag acagctttac caatttgaga caaaacagtg acttcttttt caggaacgat
     6361 cccataccaa tagttgataa atgtgatccc cacgaagaag aaacctaaga tcaatgccat
     6421 catcgtcaat gtagcagcgg cattttttgc tcttggcttc ttaaagaatg gtacagcatt
     6481 actaatcgcc tccaccccag ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa
     6541 agcgatagaa acacccggca cagccgctcc tggaagtgca gtagcttgca atggcacagc
     6601 acctgtcacg atattatata aaccaacaac gatcagtaac gagatcacaa ggataaatgt
     6661 gtacaccgga aataatagga acgatgcaga ttccctcagc ccacgaagat tcagcatcat
     6721 cagtagcaaa acgattgtca cagaaatagc gacttgatga ccgtagagtg ctggcaccgc
     6781 tgatgtgatc gcttcagcac ctgctgagac agatactgct actgtcaaca tgtagtcgat
     6841 caataaagag ccaccggaaa tcaagccagc attctttcca agattctcac tgctgacgac
     6901 atatgctccc ccgccatgag gataagcatg gatgatttga cgatatgata gcgttaatga
     6961 aatcaataaa ataataacaa aagcggcaat gggtagcgaa taccagatgg ctgcagcaga
     7021 caaagcgact aaaacgacaa cgatttgttc cgtaccatat gcaatagatg ataacgcatc
     7081 agacgacaat aatgccaaag cagcaaatcg ggttaatttg tgctcatcat tttcagcaga
     7141 ttttaaaggc ttacctacta acaacctttt taagtaatcc aatttctaaa ctcctttaca
     7201 cacaatttta cttcaagttt tatagtcgaa aatttatagt aaacaagatg ttatagtacg
     7261 ctcactgacc ttaaaagtca ataaacttta agaaaataat tggaaaaaaa cagaaaaata
     7321 tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt atgggctttt
     7381 tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt ctatcctttt
     7441 tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca ttcttactac
     7501 tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag cttgcatctg
     7561 cggtaataag ctcaaccaaa ttaaaaacat aagaagctgt ttttatttgg tcgttcttat
     7621 tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa tcagctgacc
     7681 ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat ttttttctct
     7741 aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa tatggctctc
     7801 atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga ttgccgttcc
     7861 tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg tagcatttgg
     7921 ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa atggcaagaa
     7981 cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc cgcctccgcc
     8041 agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat cgaataatgc
     8101 agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa atgacacgct
     8161 gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg taacgatcaa
     8221 gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt
     8281 cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa cacttgaaag
     8341 aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag ctgaaccgtt
     8401 atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa agaagtcact
     8461 ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga cttcttgttc
     8521 gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg gccagtcatt
     8581 gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt tttcatttac
     8641 tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt ggataaaatc
     8701 agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata ggtctgcccc
     8761 ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat tggcatcatg
     8821 gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg ccactgcatt
     8881 cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt ccgctttgct
     8941 cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag aagtgacagt
     9001 ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat taacaacggc
     9061 atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa cagctgtgtt
     9121 caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat ttagctgatc
     9181 aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca ggaactcgac
     9241 cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt tcaatacatt
     9301 gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg ttcttacagt
     9361 atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg taacatctac
     9421 actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt ttgtttggtt
     9481 tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat cttgtacttg
     9541 ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg tctcttggat
     9601 cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga tctctgggat
     9661 tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca ttttgtcatt
     9721 caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca cgaattcatt
     9781 tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga ggacttgttt
     9841 tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat ctttaacttt
     9901 attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata ctttcaataa
     9961 agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg aacttgctcc
    10021 tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat attcgatttt
    10081 tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat cctttggcaa
    10141 gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt cttttgaatc
    10201 aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt gctccccaat
    10261 ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag caattggcag
    10321 ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg catatagcga
    10381 agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga aaatccgttt
    10441 ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc tcccccttct
    10501 tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc ggcttttttc
    10561 aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct cttacatatt
    10621 gagaaataat taaggattac agcctttact ttatc
//
LOCUS       DAXWLG010000090        10576 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXWLG010000090 DAXWLG010000000
VERSION     DAXWLG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.091"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            12..84
                     /locus_tag="ACGE4T_001796"
     tRNA            12..84
                     /locus_tag="ACGE4T_001796"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACGE4T_001797"
     tRNA            88..160
                     /locus_tag="ACGE4T_001797"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACGE4T_001798"
     tRNA            168..249
                     /locus_tag="ACGE4T_001798"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACGE4T_001799"
     tRNA            267..339
                     /locus_tag="ACGE4T_001799"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACGE4T_001800"
     tRNA            351..422
                     /locus_tag="ACGE4T_001800"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACGE4T_001801"
     tRNA            439..524
                     /locus_tag="ACGE4T_001801"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACGE4T_001802"
     tRNA            535..608
                     /locus_tag="ACGE4T_001802"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACGE4T_001803"
     tRNA            618..691
                     /locus_tag="ACGE4T_001803"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACGE4T_001804"
     tRNA            711..783
                     /locus_tag="ACGE4T_001804"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACGE4T_001805"
     tRNA            811..884
                     /locus_tag="ACGE4T_001805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACGE4T_001806"
     tRNA            898..971
                     /locus_tag="ACGE4T_001806"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACGE4T_001807"
     tRNA            995..1084
                     /locus_tag="ACGE4T_001807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACGE4T_001808"
     tRNA            1097..1170
                     /locus_tag="ACGE4T_001808"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACGE4T_001809"
     tRNA            1189..1264
                     /locus_tag="ACGE4T_001809"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACGE4T_001810"
     tRNA            1266..1336
                     /locus_tag="ACGE4T_001810"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACGE4T_001811"
     tRNA            1350..1423
                     /locus_tag="ACGE4T_001811"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACGE4T_001812"
     tRNA            1448..1535
                     /locus_tag="ACGE4T_001812"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACGE4T_001813"
     tRNA            1557..1628
                     /locus_tag="ACGE4T_001813"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACGE4T_001814"
     tRNA            1651..1740
                     /locus_tag="ACGE4T_001814"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACGE4T_001815"
     tRNA            1753..1826
                     /locus_tag="ACGE4T_001815"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACGE4T_001816"
     tRNA            1845..1917
                     /locus_tag="ACGE4T_001816"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACGE4T_001817"
     tRNA            1921..1993
                     /locus_tag="ACGE4T_001817"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACGE4T_001818"
     tRNA            1998..2068
                     /locus_tag="ACGE4T_001818"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACGE4T_001819"
     tRNA            2081..2154
                     /locus_tag="ACGE4T_001819"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACGE4T_001820"
     tRNA            2166..2237
                     /locus_tag="ACGE4T_001820"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACGE4T_001821"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACGE4T_001821"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFF8387069.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACGE4T_001822"
     CDS             3826..5298
                     /locus_tag="ACGE4T_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8387070.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACGE4T_001823"
     CDS             5381..6169
                     /locus_tag="ACGE4T_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8387071.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACGE4T_001824"
     CDS             complement(6236..6904)
                     /locus_tag="ACGE4T_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFF8387072.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACGE4T_001825"
     CDS             7019..8731
                     /locus_tag="ACGE4T_001825"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387073.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACGE4T_001826"
     CDS             8734..10506
                     /locus_tag="ACGE4T_001826"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387074.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3034 a         1926 c         2454 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcaga
//
LOCUS       DAXWLG010000091        10290 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXWLG010000091 DAXWLG010000000
VERSION     DAXWLG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.092"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            151..300
                     /locus_tag="ACGE4T_001827"
     CDS             151..300
                     /locus_tag="ACGE4T_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFF8387075.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            311..1825
                     /gene="dltA"
                     /locus_tag="ACGE4T_001828"
     CDS             311..1825
                     /gene="dltA"
                     /locus_tag="ACGE4T_001828"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFF8387076.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE4T_001829"
     CDS             1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE4T_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFF8387077.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKLFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE4T_001830"
     CDS             3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE4T_001830"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFF8387078.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE4T_001831"
     CDS             3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE4T_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFF8387079.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4717..5799
                     /locus_tag="ACGE4T_001832"
     CDS             4717..5799
                     /locus_tag="ACGE4T_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFF8387080.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5799..6602
                     /locus_tag="ACGE4T_001833"
     CDS             5799..6602
                     /locus_tag="ACGE4T_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8387081.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6672..7412
                     /locus_tag="ACGE4T_001834"
     CDS             6672..7412
                     /locus_tag="ACGE4T_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8387082.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7423..8427
                     /locus_tag="ACGE4T_001835"
     CDS             7423..8427
                     /locus_tag="ACGE4T_001835"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFF8387083.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8472..>10290)
                     /locus_tag="ACGE4T_001836"
     CDS             complement(8472..>10290)
                     /locus_tag="ACGE4T_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387084.1"
                     /translation="KKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEI
                     KDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWIT
                     VYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYL
                     GTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALA
                     LGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQ
                     LLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWI
                     VLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDF
                     LKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEA
                     KNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHAL
                     EHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERF
                     DLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3307 a         1701 c         2083 g         3199 t
ORIGIN      
        1 aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa gctatgtgac aaaactgtta
       61 gtaaaaaaac ggtttctgta agctagacac atgtttcttt ttgtcaaaat tagttacact
      121 ttgtgtgagg ttatatgagg agggaatcca atgaacaaat ggcgttctaa tccaaaattg
      181 aattattggg gaaaattcat cattcgaaca attttttata gtgcagtact gctgttcctg
      241 atttatctgt atcactataa aaatattcaa ggaggaactt ttatctataa cgaattttag
      301 gaggaagagt atggaaatca aaacgattat tgaagcaatc gatgattggg gaataaatga
      361 acctgatcga gttgcttatc aggcggatga gacgcatact tttggtgaat tgaaacaagc
      421 atcagacgca cttgcttatt atttagaaaa aaaggtagaa ggcgatggac caattgtcgt
      481 ttttggcaat ttggagtttg aaatgattgt ctcatttcta ggggttgtca aatctggcca
      541 tgcctatttg ccgattgaag agcatacacc caaggagcgt atcctatcga tctttcgggt
      601 ggctaaacca tctatggtca tcagtatcgg ggactggata gaagatattc cttcggttcc
      661 tgtcataaca aaaaaagaat ttcaagaaat caagaagata ccagtaggat tttatcccag
      721 aaatagcgta aaaggtacag aaaattttta tattattttt acttctggta ctactggaga
      781 accaaaaggc gtacaaatca gtcatgataa tctagtcagc tttgttcaat ggacgatgga
      841 agactttggg atcaagcctg gtatgcattt cttagcccaa gcgccatttt cttttgattt
      901 atcggtattt tctatttatc ctgcattagt ttctggcgga atgctgaagc cgttaaataa
      961 agcagttgtt caagactttc gtcaattatt cgcgacattg ccagccttga agttgaatgt
     1021 gtgggtttct actccatcat ttatggatat ttgcttgatg gaacctacat tcaatggaga
     1081 aaatgtgccg gaattggaca tgtttttatt ttgcggagaa gaattgacga aaaaaactgc
     1141 agaaagtcta cttgatcgat tcccaaatgc acggatatat aatacttacg gtccaacaga
     1201 agcaactgta gctatttcaa gtattcagat tgaccagcat gtactagata gttatgaccg
     1261 actaccgatt ggctacgtaa aaaaagacac gaaagtttct attgtccaag aaggtaaacc
     1321 tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga ccaagcgtat caaaaggtta
     1381 cctgaacaat cccgaaaaga cagctgctgc gttcttttgt caaaatgact tagcaagtta
     1441 ccatacaggg gatgcaggta gactagatga atatgggctt ttattttatg aaggacgtat
     1501 ggatcagcag gtcaaattac atggataccg gattgaactc ggggatatcg aacattatct
     1561 gctccaagac aaccgaatca aacaagctgt cgtcgtacca aaataccaag gaacaaaagt
     1621 acagcagctg gttgcttttg tcgtattaga aaacaaaacg aaacagccgg attttcagtt
     1681 gactaaatct atcaaagaac aattactaga agttgtaatg gattatatga ttcctcagaa
     1741 gttccattat gtcgatctgc tgcctcagac agttaatgga aaaatcgatc gaaaaaaatt
     1801 aacagctgag gtgaatcctg aatgatgtta cctcatatga tcccttatgc agagccgttt
     1861 tatttttttc ttttagccat tgcgcttgta ccgatcgttc tatctttatt gatatgggaa
     1921 aaaagattac cagtttatca atcagcagtg acacttttct ttttatttgt cagctttgga
     1981 ggtaattatt ggcaacaggg agcggcgctg attgggtatg ttttgtggca gacgatactt
     2041 gtatggggct attttgttta tcggcaaagg aaaaatcaga caggatggtt ttttgccgcc
     2101 gttattttag cgataattcc actgttttta acgaaagtga ctcctttttt tgaacatgga
     2161 aaagcatcgt tacttggttt tttaggaatc tcttatttaa cctttaaatc tgtacaaatg
     2221 atcatggaaa taagagatgg catgatcaaa acatataagc catttcgtta tatccagttt
     2281 ctgctgttct ttccaacaat ctcttctgga ccaatcgatc gctataggag atttgaaaaa
     2341 gatcagctcc atccgcctgt tcgtgataaa tatgttgatc ttttgggaaa aggcgtccaa
     2401 aatatttttg tcggtttttt atataaattc attatcggtt attattttgg tcaagtactg
     2461 cttcctgtag tgggacgcat tgccttacag catggtggta tttcatgggc attagtaggt
     2521 tatatgtacg tgtacagtat gtatttgttt tttgattttg ctggctatag cttatttgca
     2581 gtcggtacaa gctacatgat gggatatcag acaccaatca atttcaataa acttttttta
     2641 tcatggaata tcaaagaatt ctggaatcgt tggcatatga cgctgtcctt ttggttccgt
     2701 gattatgtat atatgcgatt gatgttcttc atgatgaaga aaaaattttt taaaagcaag
     2761 atcgtaatgt caaatatcgg ttattttgct ttgtttcttc taatgggtat atggcatgga
     2821 ttgacgtggt actacatcgt atatggactg tatcatgctt gtctgatctg tctgacagat
     2881 gcatggatcc gatttaagaa gaagcataaa gagcagttgc cttctaacaa atttacgcat
     2941 ggcttagccg tatttttgac atttcaagca gtatgtttta gcttcctgat tttttcagga
     3001 tttttagata aacttttttt tagctaacga atagtgacat attaatttat ataacaaagg
     3061 agaaaaggaa aatggaagaa caaattttat caatcttaga agaaataaca ggaacagatg
     3121 aagtaagaaa aaatcgtgat attgatctat ttgatgaagg gttgcttgat tctttagcat
     3181 ctgttcaatt attagtcgag ctagacggag agctggatat ccaagtccct gtatctgaat
     3241 tcgagcgaga ggattggagc acaccgaata aaatcattca acaagcaact gcattgaagg
     3301 gttgagtatg aaaaagaaac tttttgctat tttcggcccg gttgttttag ctgttggctt
     3361 actgtttgtg ttttttacct cctcctatca agtagatatc tctaatccag aaacgattag
     3421 acgtgcctct tcttccatgt cagtgaacgt gttgaaagga gatgcgataa aaaattatgc
     3481 attatctgaa aaacaatata ttcctttttt tggctcaagt gaattaagca gaatcagtcc
     3541 atttcatccg tctgttctgg cagaaaaata ccagcgaaac tatcgtccat ttttgctggg
     3601 agcacccggg acacaatcgt taagccaata tatgatgatg cgttctgcag gagatgctat
     3661 gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg ttcgttaaaa atggtgtaaa
     3721 aactgattac tttaatacgt attattctga acttcaaaca tacgactggt tgttttcaat
     3781 gaagaaagta acgcctgctg atcgctattt agctagaaga ttattaactt tctcaaaagt
     3841 aaaagagaat gatacgttga cggcgattct gcaaacgatc aaaaaaggga agctaccttt
     3901 acctgaatct ttgaatcagc tgcgcagcca atggaacatg ctgaagagag aggatgaagt
     3961 attcagcaat attgggttga aagatcgaca gcaaaaaatc gatcatgagt caaaacgatt
     4021 accaaaacaa taccaagaaa cagaattatc cattttagca aatcaaattg gagaacgaga
     4081 aacgacgaat aatccattcg ggttgaaaaa cgatttttat acgcatcgta tccgagccca
     4141 cgagccagag ctcaaacagt cacaaaaaaa ttgggattat cgtttttcgc cagaattttc
     4201 tgattttcag ctggtcttgg atcaattagc aaaaaaccat aacgaagtat tgttcatcat
     4261 cccaccggtt aacgagaaat ggagcgatta taccggacta tctcaagaaa tgttacaagg
     4321 atttgccaaa aagatcaaat ttcaattaaa tagccaagga ttcaatcgaa tagctgattt
     4381 tgtcaatcaa gcgggtacca actattttat ggaagacacg atccatctag gctggaaagg
     4441 ctggttggct gcagatcaac agatcagacc gtttttagaa gaaaatcata ttactgctag
     4501 taagtatcat cttgacgatg ctttttttag caaatcttgg caacatcaaa taccagataa
     4561 actacaatta aaataaagaa aacaggcgag tgttgctcgc ttgttttctt tatttttgaa
     4621 tattctgaat aaaagataaa aataccaagt ttgcctgaac aaaaatcata agcaatggta
     4681 taatcacaat atgaatatag aaatggggga aaacatttgg atacaaaaga agaacagcta
     4741 cttattgatt taacttctgc aaaaggagta cctggaaatg aagaagaggt aagagaagta
     4801 ttcagagaat atgccaaacc attcgctgat gatatttttt atgacggttt aggcagtgtg
     4861 attgccaaac atggtgctgg cggaggcccc aaggtcttta tttctggaca tatggacgaa
     4921 gttggtttta tggtaacaaa aataaccgaa aaaggattct tggaattcca aactcttgga
     4981 ggttggtggg ggcaagtcat gcttgctcag caagttgaaa tcaaaacacg tgaagggaaa
     5041 atcgtccacg gtgtgattgg ttccaaacca ccacatgttt taacacctca agtaagaaat
     5101 aaaccttatg aaataaagga tatgttcatt gatattggcg catcaagcca agaagaagcc
     5161 aaagagtggg gcatacgtcc tggagatatg gtcactccat atattgaata caaacgaatg
     5221 aatggcagta aatatttact ggcaaaagca tgggataatc gtatcgggac agctgtttct
     5281 ctgcgagtat tagagaatct atctaaagaa gcacacccaa atgttttatt cgcaggaagc
     5341 gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa gtacacacct tgtaaatccc
     5401 gatattgctt ttgctttaga tactggtaca gcaggagaca ctccaggaat gacaccaaaa
     5461 gaagcagatt cgattttagg aaaaggccca cagattttga tttttgatgc atctatgatc
     5521 ccgcataaaa aattattgaa ttttgttatt gatgtagcag aggaaatgga tattccattc
     5581 caatatacag tgatttctgg cggaggaact gatgcaggac aaatgcattt gacaagagat
     5641 ggtgttccgt ctgtagccat taccgttcct gttcgttatc tgcattccca tacatcagtg
     5701 attcatgaag atgattattt gaatacagtg aaactagtca cagaattagt gaaacggctt
     5761 gatagtgaaa cagttgaaaa aattcggagt tatgaataat gaataataga atacctattt
     5821 ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc aatacttggc
     5881 ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac ggtttcagtc
     5941 cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat aatttacttt
     6001 tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg ggtggcagac
     6061 caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg atacttggca
     6121 tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt ctgcccatgt
     6181 ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat gcggtaccta
     6241 cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg atagggatga
     6301 tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt gctgcatgga
     6361 caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc tggtttttat
     6421 tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc tggtatttgc
     6481 taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg aatgtgtcag
     6541 ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc tattttactt
     6601 agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa atctttgagg
     6661 gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct tataaaaatc
     6721 aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc gatgcaggta
     6781 gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat gctgtgatcc
     6841 ttagccatta tcatcatgat catattgctg accttggtgt attacagtat tactggcaac
     6901 ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca aaagattcca
     6961 ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat tttgaagctg
     7021 aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat ccagtagtat
     7081 gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt acaggtgatt
     7141 caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt ctagcagata
     7201 cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa gaagctggag
     7261 aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct cagtttggtg
     7321 atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt gatatagaac
     7381 ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt tgtaccaaat
     7441 gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca agaaatgcct
     7501 ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat cggcagaaac
     7561 caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat tcatgttgtc
     7621 cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa ctttagtttt
     7681 atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca gccaatcatc
     7741 caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa tgatctagtc
     7801 attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg gatgtttgct
     7861 catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt aaaagtaaaa
     7921 aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac gaatatcaaa
     7981 ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca agaaatcttg
     8041 ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt gactgatgaa
     8101 gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga ttggaattac
     8161 gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg ttcgattgaa
     8221 gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg cgatttcttc
     8281 ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata tgataaagca
     8341 tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc gatagaacca
     8401 gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac atatatggat
     8461 tccgctattt tttatacagg attcgggtaa tttcgagaga gttgaacagt gatccgatca
     8521 atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac gatccgctac gtctttcatc
     8581 atcattaagt caaaacgttc ctgatcatct aattcttctg tgatgcctcg actaaatagg
     8641 tttgtatcgt aatgtgcact atgttcgcaa caaatgacga aatgttcctc ttgttcgtgt
     8701 atcgacaaat aaatttttcg atacagtgga ttttcgatgg catgagtttc tctaaaggaa
     8761 tgctctaaag cgtggatcaa aagttgagcc agatcgtcat cgttgatagg aagttcttcg
     8821 ggaagatctg cttggaaggt tatctgaata ttccgtttgg cagctaactt ctggtaacgc
     8881 gctaagataa gatttgtcac ctggtggttg caaaaagaac acagcatttc gaaatttttt
     8941 gcttcttcta atatcttttc cacatagttt ttagcttttg tttggtccgt atgcattaaa
     9001 tcttgcagat tctccatctc ttggacaaat tccaggcgaa gtgtatttac ttcttggata
     9061 tgctgtcgaa gctgctcata atgttgctcc aacaaatcat ttttgatttt taagaaatct
     9121 acttgtctcc gatctttgtc ggtatttaag atgtgatcga ttagattgta gctgataata
     9181 acgattagaa tgataatata taaaatcgta gaccaattga tcgttgtttg atagatacct
     9241 aatgccgttt ggatgtaaat caagcaatgg ataatggcta ccaagacgat ccaaggtgta
     9301 caagctacat aaaaacggtt attcttatat gcttcgccaa tgcatgcaag tgaagtgact
     9361 aaagtgctta taacactagc aatattagca atcggcatta attcaggaag ttctgcagga
     9421 gtaaaagttg cataaagcag aacgccgata aaaatcaaaa gttggaacgt acaaaaaatt
     9481 ccgtaccatt ttttatagta aaacattcgg ctccaatagt agcagatgat gaaagctgac
     9541 gttaagataa taaataaatg agataaaaaa gaatgaacga atggttcaaa aagaatacca
     9601 cttaaaatat cttctgatac agcttcaaat cctaatgcca aagcaaagca acttaagaga
     9661 ataagtgagt attcaagacg tttttgttta tacatcaagt agatcgtata gaaaaaggtc
     9721 cctatagcga taaaaacagc gattatcatc gtcaatactt gcggcactga ttgtgaataa
     9781 ataaaactaa tcaatggtcc agttgttcct agataaactt tcggtggcag tccagcatag
     9841 ttctgataag gagatgagac tgtaagggtc aatttttttc cgatataatt tgctggtaaa
     9901 tctatcacag ccaatgagag tccgggattg ctttcttcct gataatcttt ccgttggtat
     9961 aaaatctttt cgcctagata tacagtaatc cattgatggt ttcctttgat tagtaaattt
    10021 gcttcgtcca acggtttttc aagtgtcgtt tccataatga atgtttcgtc tttatccacg
    10081 gtaggaatcg tacgagtgta cttgctgtca aacttaaaat caggatcatc ttttgtatag
    10141 aaaacccacg aatcttttat ttctttcatt tgattgctgt ccgcaggaga aaaaataaaa
    10201 taaaagaaat aagcaattcc tgttaaaaat aacaatgata atataatgat tatgctccac
    10261 tttatacgta aataaaaaga ttctttttta
//
LOCUS       DAXWLG010000092        10255 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXWLG010000092 DAXWLG010000000
VERSION     DAXWLG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.093"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            2..2698
                     /locus_tag="ACGE4T_001837"
     CDS             2..2698
                     /locus_tag="ACGE4T_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8387085.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2775..3263
                     /locus_tag="ACGE4T_001838"
     CDS             2775..3263
                     /locus_tag="ACGE4T_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8387086.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3275..3769
                     /locus_tag="ACGE4T_001839"
     CDS             3275..3769
                     /locus_tag="ACGE4T_001839"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8387087.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3790..4599
                     /locus_tag="ACGE4T_001840"
     CDS             3790..4599
                     /locus_tag="ACGE4T_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8387088.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4596..5417
                     /locus_tag="ACGE4T_001841"
     CDS             4596..5417
                     /locus_tag="ACGE4T_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8387089.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5468..7567
                     /locus_tag="ACGE4T_001842"
     CDS             5468..7567
                     /locus_tag="ACGE4T_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFF8387090.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7564..8682
                     /locus_tag="ACGE4T_001843"
     CDS             7564..8682
                     /locus_tag="ACGE4T_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFF8387091.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8721..9599
                     /locus_tag="ACGE4T_001844"
     CDS             8721..9599
                     /locus_tag="ACGE4T_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8387092.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9745..9942
                     /locus_tag="ACGE4T_001845"
     CDS             9745..9942
                     /locus_tag="ACGE4T_001845"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8387093.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            9926..10156
                     /locus_tag="ACGE4T_001846"
     CDS             9926..10156
                     /locus_tag="ACGE4T_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387094.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
BASE COUNT         3147 a         1680 c         2286 g         3142 t
ORIGIN      
        1 attggaatac aaatatagaa tagacatatt gcagccgata gtttattatg cttttagaca
       61 cattaaactg gaggaaaaaa tgaaaaagag cgctaaagat cgagtttttg atgagctgat
      121 acgactaaca gggaatatgt cttctgtcac tgccgagaaa atttcgaaga atctaggtat
      181 ctctcgtcag aatgccagcc attatttgac gagattagtg gaagataaaa aggtcgagaa
      241 attaccggga aagcctgttt tgtggaagcc tttagatgaa tatgcggttg tagataatac
      301 ggagcaaata aacgaagcct ttcattctgt agttggtcat gacggaagtc tgagagaggt
      361 cattcaaaaa tgtatagccg ctgtcaaata tccgcctaac ggactcagtg tattaataaa
      421 tggagccacg ggtgttggaa aaagtttttt agccacaaaa atttttgaat acgctatcca
      481 tgagcaaatt attgaaaaag atgcaccatt tgctatctta aactgtgccg attatgcgga
      541 caatcccgaa ctgctttcgg caacactgtt tggatacaag aagggggcct ttacgggggc
      601 tgaaaaggat accgatggtt tattggcaac ggccaacaac ggctatcttt ttctcgatga
      661 ggtccatcgc ctatcgaaag aaaatcaaga aaagctcttt ctatttattg atactggaaa
      721 ctatcgtcca gtcggtgaaa atgtaaattg gcattctgct aaggttcgtt ttattttcgc
      781 cacgacggaa aaaggtgaaa attatttatt agacaccttt gatcggcgta ttcaaattag
      841 tgttatgttg ccaacttttg aagatcgccc cattcgagaa cgattagagc tgattcaatt
      901 attttttcaa aatgaagcgg atgttttaca aaaagatatt attgtttcaa gagaagcctt
      961 gtctttaatg ttatctcatc cctttagtgg gaatattggt aagctgaaga atatcattaa
     1021 aatcagttgt gcggatgtgt atagtcgaac acaagaagaa ccattaacga ttgggaaaaa
     1081 tgagatcttg attcagttaa atatgctgga atccgaagta gaaagcttgc cgattgctag
     1141 tttattgata aagaaaaatc aaaagataaa aacggatcga atcaaaccgt ttgattatga
     1201 ttcgattttg gaaaaaatta ctcagctgat aaatgaaggt aatttcaatg aaaatttttc
     1261 ttcgataaag aatgaagtac agcatttggt gcatcacatt gataaggatt cttttttatc
     1321 aatgagtaga aaattgatga ttcagatgtt tagaaaggtc tgggtatccg ttttaggaaa
     1381 aacgtacgga ttgacgacga gtggacctgt tgcagaaatt attagcagat cctatgtcat
     1441 ttccccaagc tataagacag acttaaccgg gataaatcgt acattagctg caaatctacc
     1501 tcggacgact tacttatgta atcagttctt ggagcattta ccaccgctct cagaaaagga
     1561 cgagaacttt ctaagactgc ttattgtcgt gactttatct gattatgtag atgaatccat
     1621 cgagctgaag ggcctgttgc ttgctcacgg agatagtacg gctagcagca ttcaagcagt
     1681 agtgaatcaa ttatgcggca attatgtttt tgaagcgttg gatatgccag tagagagtaa
     1741 tgtgtcagag attgtcgaat cgacaaggaa tttcgtgaaa cggcaagtga atacagatag
     1801 tctagtgctg atcgtagata tgggctcttt aaatcaaatt tacacacaga tcaagaacga
     1861 tttaaaaggg gagttacttc ttctgaataa tttgacgact tccattgcac ttgatattgg
     1921 cttgaagatg acgatgaatg cgccatttaa acagattgcg gaggatgcag cagacaagta
     1981 ttcaatcaat gttcaatact ttgaaggctt ttctcaaagt agcaacatta tcatttcctg
     2041 tatgtcgggt ttggggattt cggacaaatt gaaagatgtt tttcgtcatc atttatcgga
     2101 tgaatccatc gaagttttca cgaaggacta cagagaacta cgtgaattaa tcgaccaaaa
     2161 tgatgaaact tattttgaca agacaaaatt ggtcatcacg acttcggatc ttccacgttc
     2221 cttttcaatt cctaatatta atgtttatga catcttagac ggtgatggag cagccaattt
     2281 ggagcatatt ttgagtcctg aaatgaagcc ggcagaattt aaccgcttga ttcaggagct
     2341 agtaaaattc ttttcgttgg aaggaattgc ggaccgtttg aactttctga acccaacgat
     2401 cgtgatgagt gaagtggaaa cagtcatttc taaatatgaa aattactatc atttcacttt
     2461 aaatggccgt gtaaaactaa atctctatat gcatacggca ttgatgatgg aacgtttatt
     2521 cttggctcgg acagaaaaat ctgaagaacc gaattatgat ttgaaaccag atgaagatga
     2581 attttattat gtttctagaa gtatttttca atcaatggag atgaaatata attttaaagt
     2641 caatgcttat gagctttcgc tgctctatga attattagga ccgtatattc aaaaataaag
     2701 tgtctaaaaa agtgtctaat ctgttttttt taggttagac acttttttta atgtgaatta
     2761 acgtgattaa accaatgttt ttaaaagttg gcacaggaat tgcttatata aaagtgaaca
     2821 gaaagaaagg aattaaccat atgaagaaaa ttctaatagc gactcacggt cacttagctt
     2881 caggctttct tagcagtatt caattactga cggggaaggt tcaagagatt acggtgatta
     2941 atgcttatgt ggatgagtgt gattttgaac aggagttgaa tgtatttgta aaccaggtga
     3001 acgagcagga tcagattttt gtttttaccg atttatttgg cggaagtgtc aatcagaaga
     3061 ttaccaaaac ctttttggag aaagagattg ctgtaactat tttgtctggc tttaatctgc
     3121 caattctgct cgaaatagta ttggcaacca aggaattaac aaaggaggat attcaggaac
     3181 tggtaaataa atgccaacag gaattaaaga tgaccattct tgatgaggta catgtagtaa
     3241 atgaagagga agattttttt taggaggaag aaaaatgatt aaagcagtgc gaattgatga
     3301 acgattaatt catggacaag tagcgatgac ttggacaaag agtctaaatt taaccgggtt
     3361 agtagtagca agcgatgagg cggctaccaa cgatattcaa aagatgacat tgaagatggc
     3421 tgcaccatcg aatgtcaaag tcattattaa aacagttgat gacgcgattc atttgttaaa
     3481 agatccgcgg gcgaaggaca tgcgcttgat ggttttagtc cctaatgtca aagatgcggt
     3541 aaaagttgcg aaagctttcc caacggaaat cgaattaatg aacgtgggga atgctgggaa
     3601 aatgacaccg ccatcggaag ataagaaaac cttatcaaag gaagtgatgt taacgacgtc
     3661 agaaattcaa gacttaaaag aattagttga actttatcca gataccgttt ttcagcctac
     3721 accggcgatg gaaaaacgtt cagctatctc tattttgagt gatttttaga aaaaggaagg
     3781 agaaaaatta tgtatgatgc atttgtagtc gctttggccg tttttattgg agttgctgga
     3841 catgaaatgt tcgggatgtc catgctgagt cgtccgattg ttgttgctcc gttagcagga
     3901 gcgttgatgg gggatttgca gacggggttg attgtcgggg cgtcactaga agccatcttt
     3961 atgggtgtcg tcaatatcgg aatctctagt acggcggagc cttctttggc tgctgggctg
     4021 gctacagcgt tcgcaatcaa aatgggagga aacgtggatg cgattattcc cattgtcttt
     4081 ccactagcag tattaggatt acagttaatg aatatgattt tttcttttgt atgtggaccg
     4141 atggctgcta aatttttcaa atttgcaaaa tctggtgatc aaaaaggaat catcaatctg
     4201 cacttcttta tgtggtttgt ccactatgcg ctttatgctc ttattccatt ctttgccgtt
     4261 ttatttggag cagatgttgt caaaagcgtc ttggataata ttccagaggt catcatgaac
     4321 ggattaacag tcgctggaaa tctgctgccg gcagttggga tggcgatgct gttacggatg
     4381 ctttggagta aaaatattgc tgtgtggttt ttctttgggg ccgttgttat ggcgtatctc
     4441 gagttaccat tgattgcaat tgccgcagtt ggaacgatta ttgcaattgt tatagcccaa
     4501 agagatctac aactaaaaaa tatatctgtt catgcagttc aaagcgatgc tgctgtaaat
     4561 gcaacgcttg accaagaaga ggaggatttc tttcaatgaa attagctgat ggaatttcta
     4621 aagatgagaa aaaattggtt cggaagatgt tttggcgctc gatgactatg tatgcttcgg
     4681 tgaatccggt gacgatgggc ggcggtggtt tcgcctattc tatgatgccc ttcattaatc
     4741 atttctacaa aagtgaagaa gatcgagctg cggcgctgga acgtcataca cagtattttt
     4801 cgacgaccat tccttgcgcg tcctttgtca tggggattgc ggcatcgatg gagaaggaaa
     4861 attctgaaaa agctggtttt gacgtggaaa gtatcaacgc gatcaaaacg agtttgatgg
     4921 gaccattggc tggaattggc gattcattgt tctggggagt ttggcgtgtc gttaccgcgg
     4981 gactagcgat caacttagcc aatgccggaa atattttggc accaattatc tttttagtta
     5041 tgttcaatat acctaattac ttgatgcgat actatggcgg cgttttaggc tattcattag
     5101 gctctaaata tattgaaaag ctgtatgcga acgggttaat tgaaattctg acaaaagctg
     5161 ctagtatctt gggtctgatc atggtaggtg caatgacgat cagtatggtc aaattcaata
     5221 ccgttttgag cttttcaatg aagggcgaag aactaatgaa tgtccaagaa atcctcgata
     5281 gtatttttaa aggaattgta ccgttattgg taacattgag ctgtttcaaa ttactagata
     5341 aaaaagtcgg cgtcatcaaa attatttttg gaatggtcgt cttgggaatt ttattgtcat
     5401 tacttggaat cgcatagtag gaagctgcag ctactaggct gcagtttttt tagcaaggag
     5461 ggaaaagatg actattaatg aagtaattta ttcacccaaa cacccatttt ttaatgctga
     5521 gttaaacgac ggaaatgtga ctacttcaaa cgaatacagc tactatcgga atattcctga
     5581 tgtaaaggga ttgaccttcg atgtaagtaa tcaaaagaca ttagcaaacc ttgatgtttg
     5641 cgggacgatt caaacgttga ccttttatcg agaaaattta ctaactgagg aaaaaccagg
     5701 tgtttgggtc aataaacagc tctctcaaac caattcgtta tccttaaagg ttgaagtaaa
     5761 cgggattgtc taccatttgt cagaatcgga tcatcgtatt gaagtcgatg tgattcaaga
     5821 ctgtttgcct agatatattc atcattattc gacgtttaag gtcattgtag tatcctttgc
     5881 tcccatactg gctaataagc gtttaagtat gcttgttcaa caggtttaca ttgtgaatga
     5941 aacacacgaa agccttcaag tttcagtgaa agatgtgcct tcgtatcaag agaagtattc
     6001 ggatcagcaa aatgtgttga tttctcaaaa aggaaataag gacacgatcg cacaacagga
     6061 agtggcaagc ttcgccattg cttttgtgga cccaaatgct tttgaagaag taatgacttt
     6121 tcaggaatct gatatcgaaa aatggctgaa ggaaacattg aactattttg ctcagctatt
     6181 tgggcagctg tcaatgccaa acgatagagt tgtccatctc tatcgtcgtg cactttatca
     6241 aagcttttct agtttcggta tgaatcgaca gggacaggtc gtcggcagca attggggcag
     6301 ctatcctgct actaatcgaa tttggaataa ggatatgtat tacgcatcat tgccattcac
     6361 catgtttgaa ccggagttat gtcaaaaaac ggttctatgg tttgatcaat atggcgttaa
     6421 gtttcctggt tcaaaattta caggaggtat caatcattct ctgagtaatt cgttatccag
     6481 tgcgttactg gctagtcttt attttgaaca tactggggat acaaaattct ttgagcggtg
     6541 tccgcaggtt ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc
     6601 aggggaacca atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta
     6661 tcataccggc agcaatcttt gtctttggaa ggcctgtatt ggacttgcag aaatttttga
     6721 agccctagaa caactgagtc ttagcaagcg atacaaaaac attgcctgta aactaaagga
     6781 atcaatttgt aaacaaatga ctatcgatgg tccttttggt gagcaatttt tggagggaat
     6841 cggtgatgag gagaaaacaa cctattcagt gaagcactat caaaaaccga ttcttgagca
     6901 agggttaatc tttttaagtg atgtgattgt ggatggaaaa attaatttat tgatgcatga
     6961 tggagaagaa tcagacacta cgttgatgcc tttttatcaa tttttagata ataaagatca
     7021 tttatatcag aacaccatgg ccttttctgc tagttctttc aatccaacct atagcgagga
     7081 aatcaaaggc atcacttggg gattagaatc cggcgccact tttcctggct ttatcactgt
     7141 tttaatgagc gagctgcaca accctcaagc tttcgtaacc cgattagaag aactaatttg
     7201 tttggcagat ttagacggtt cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg
     7261 agaagtcgtg cgagactttg gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag
     7321 cctaatgatc agtcaatatt tcggattgaa attcaaaaaa ggagtattgc aaattgatcc
     7381 tgttgataat ttaacgtttt cttggaagaa tttgaagttc ggtcaagcct tcatttcgat
     7441 tgaatgtacg caatccgagt tgagtatttc aaatttgtca gaaaagccat tgaaaataag
     7501 tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg attcacattc aaaggagaaa
     7561 aagatgagta tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg agcaatcact
     7621 cccgaaaatc caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca
     7681 agccgcaaag gtcgtggcgc aattccgctg acttctggtg aagaaactgt gattgcggat
     7741 attactggaa cgggaataat tcgtcatatg tggatgacga ttcgtgatcg aacagaattt
     7801 ggcagttttg ttttacgtga tttaatctta agaatttatt gggatgggtc ggatagccct
     7861 gctgttgaaa gtcctttagg ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg
     7921 aactcgttgc cgatagtggt taatcctaca gggggcttta attcctattt cgaaatgcct
     7981 ttccaaacat ccgctaaaat tacgatcgag aatttacatc cgaaggatat gtcctcattt
     8041 ttttatacga taaattacgc tttggtggac gaattggaag aaaatagcct atattttcac
     8101 gcaaattggc atcgggaacg cattaccaaa ttacaggagg attataagtt aattgatcga
     8161 ttaaagggtc aaggctatta cgtgggtact taccttgcgc ttacctgcct agaacgttat
     8221 tggtggggcg aaggcgagtt taaattctac attgatgggg atagtgagta tccgaccgta
     8281 acctcaacag gttcagaaga ttattttggc ggggcgtggg cctttcatga gtacgatgag
     8341 ttcggacgtc cacatgcgaa aaactattca acagcctttt tagggtatcc ttaccaatca
     8401 aatagagatg ccacaagaga tcgttttgaa acgggaaaat tgaatgccgt tcatgccttt
     8461 ggtgacgacg caatgccacg acatggtctt tatcgttggc atcttcaaga ccccatctgt
     8521 tttaccgaag atttatcggt cactttacaa caaatcggca acgatgatgt tcgcttattt
     8581 gagcggcaag acgatgttgc tagtgtcgct tactggtatt caacagaagc ttcaggaaat
     8641 actaaacgtc atttcactga tcgagaacgc ttgccgcgtt aatgaagtgt ttttcacata
     8701 ttcgaaggga ggaacctatg atgatatatg gggcaattga agctggaggg actaaatttg
     8761 tctgtgcagt cggtactgag gaattaacga ttttggaaag agtcagtttt cccacaacaa
     8821 ctccagttga aacaatgaag caggtcattg atttttttaa accttttaag gaacaattgg
     8881 caggcatcgg ggtcggctgc tttggtccaa ttgatattca atgcaaatca acgagtttcg
     8941 gtcatatcac atcaacacca aaacttgctt ggcaaaattt caattttgta ggaacgctaa
     9001 agcaatattt caatattccg attgaatgga caacagatgt caatgcggct tgctatggtg
     9061 aatatgttgc tggttctggc aaagggttag caggtgttgc atacttcact gtgggaacag
     9121 gtatcggagg tggtgccttg ttcaatggga ctttcgtgga agggttcagt catccggaaa
     9181 tggggcatat gttgatcaag cgacatccaa aggacaattt ttcaggaaac tgtcccttcc
     9241 accaagattg tttagagggg atggcagcag gaccagcgat tgaaaaacgg ttgggtgtaa
     9301 aaggccagaa tttattggct gacgattcgt tttggcagat tgaagccttt taccttgccc
     9361 aatgtgcata caatacaaca ctgatgtttt cgccagatag aattattttt ggcggtggtg
     9421 taatgaaaca agaacacatg aagaaaaaag ttcaggataa atttgttgag cttataaatg
     9481 gatatgttga aataccacca attgatagct acattattac gcccgaatta ggtgataatg
     9541 ccggaataat cggcggatta gcattggcca gaaaagcggt aagaaataag caaccataat
     9601 taatgaatgt ttcattgata ctcatgtcaa aagtatttaa ccgagtaatc aaatgatcaa
     9661 atgaagttgg ataaagcgaa aatgaattca aataaaaagg ctctaagtca aatcgtgttg
     9721 ttttcattaa atcactgaaa ggaaatgctt ctgcgtttcc ttttttcttt ttgttgtgcg
     9781 ctgtatacct tgaggatatt cactggtggc gtacacttaa atagtaatat attgtgttac
     9841 tttatgtctg tggtaatagt tatagttgct taccaatgta tgttcatttt tcgaaatgtt
     9901 gacttcataa tccttggctt aatgtttgaa tttttttggt gatagatttt ttcagccgta
     9961 taaaataaat cgtaattcta ttatagagag ttgtgattat aaaaagatag caagaaaaaa
    10021 aaagaaagtt tctatacatt ttaaatattt tattcctatt tctattgcta aaaaagtaga
    10081 aattctatat ttacctttaa ctctcgcttc tcttgcaaat gtagttagca ttttaatcga
    10141 taataaactc aggtagttat attaccttga aatgattgat aattttgtca cttctatttt
    10201 ttttgcaata aattttttat tttagtgaaa aataagaaac tatttctttg aaggc
//
LOCUS       DAXWLG010000093        10069 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXWLG010000093 DAXWLG010000000
VERSION     DAXWLG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.094"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..223)
                     /locus_tag="ACGE4T_001847"
     CDS             complement(<1..223)
                     /locus_tag="ACGE4T_001847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387095.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISV"
     gene            complement(216..845)
                     /locus_tag="ACGE4T_001848"
     CDS             complement(216..845)
                     /locus_tag="ACGE4T_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8387096.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(838..1956)
                     /locus_tag="ACGE4T_001849"
     CDS             complement(838..1956)
                     /locus_tag="ACGE4T_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFF8387097.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1958..2590)
                     /locus_tag="ACGE4T_001850"
     CDS             complement(1958..2590)
                     /locus_tag="ACGE4T_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFF8387098.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2601..4418)
                     /locus_tag="ACGE4T_001851"
     CDS             complement(2601..4418)
                     /locus_tag="ACGE4T_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFF8387099.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4431..5195)
                     /locus_tag="ACGE4T_001852"
     CDS             complement(4431..5195)
                     /locus_tag="ACGE4T_001852"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFF8387100.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5223..5921)
                     /locus_tag="ACGE4T_001853"
     CDS             complement(5223..5921)
                     /locus_tag="ACGE4T_001853"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFF8387101.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5933..6712)
                     /locus_tag="ACGE4T_001854"
     CDS             complement(5933..6712)
                     /locus_tag="ACGE4T_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFF8387102.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6728..7672)
                     /locus_tag="ACGE4T_001855"
     CDS             complement(6728..7672)
                     /locus_tag="ACGE4T_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8387103.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7718..8335)
                     /locus_tag="ACGE4T_001856"
                     /pseudo
     CDS             complement(7718..8335)
                     /locus_tag="ACGE4T_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8462..9763
                     /locus_tag="ACGE4T_001857"
     CDS             8462..9763
                     /locus_tag="ACGE4T_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8387104.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(9813..>10069)
                     /locus_tag="ACGE4T_001858"
     CDS             complement(9813..>10069)
                     /locus_tag="ACGE4T_001858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387105.1"
                     /translation="KAVIFMYVNYILIYLSSILRSESMEKPFVKSLTAVAVGAGAIAI
                     CLFGYHINNQRQHQQRINYAESAITNQKDTLTSLSRLFVK"
BASE COUNT         2788 a         2080 c         1734 g         3467 t
ORIGIN      
        1 ttacggatat atttttttta tatccgatcg tttttatcac atgacgattt ttaatcatat
       61 gcctttcttt actggataga aattgttttc ttatatgtga gtaatcagat acaacattaa
      121 aatattcgat actgttttcc gtcagaattt cctctaatat attcgttcga ctgatataat
      181 caaaaggatg aataagcagc cacttttttt tattctcact catctatctt cgcaccctca
      241 aatttctcag tagtcccact tgctactgat gttattccat cacggacaat aactttcttt
      301 agcgttaaaa aaaatatttt tatgtctttc atcattgaaa tgttctccac atattcaata
      361 tctttttcaa atttttcttt ccaactaatt gcattacgac cactgacttg agctaatccg
      421 gttaaacctg ggcgcacaaa atgtcttttt cgttgttcat tactgtatag tgaaagatac
      481 tcaactaaca gaggacgcgg tccaataata ctcatatctc cttttaaaat attccataat
      541 tctggtagtt catcaagact agtcgaacgc aatattttac caaattgagt caatctgata
      601 tcgtctgata ataaattacc ttcttcatct ctttgatctg tcattgttcg aaatttatac
      661 atcagaaaaa tcttttcatg ttttccaggt cgttgttgtt taaagatcac tggcgatcca
      721 agctttattc gaactaggat tgcaacaaac gtcatcagtg gccataacat tattaaacct
      781 aaaattgaaa aaagaatatc tatcaaacgt ttaaagaact tttcgtagat acctctatta
      841 gacattttgc atcactcgct taattgtatt agttactcga tacaaatcat catcggtcat
      901 tttagaatct gaaggtaaac agataccttt ttcaaacaat gtctgagcaa catctgtacc
      961 aatatactca tacttatcga aaactggctg taaatgcatt ggtttccata tcgggcgggc
     1021 ttctatatta tttttatcta actcatcaat aagatgattc gtatttattc cctttactgt
     1081 tatgacactt aaccaataat ttggttcatt ccaattattt tctggcatga attgtatatt
     1141 tgacaattta gacaattctt ttttataaaa actatgaata tatcgttttt tctcaattct
     1201 ttcatttaaa actttcaatt gtcctcgacc gatacctgca acgatattgc tcattcgata
     1261 attatagcca atttcattgt gttgataatg gcgtgccttc tcacgagatt gcgtagccca
     1321 aaacctcact ttttccacct tttctttgtc attagacaca agcattccgc cacctgaagt
     1381 cgtgattatc ttatttccat taaaagaata aattccatag tcgccgaatg taccagtata
     1441 ttttccttta tatgttgttc ctaatgattc tgcagcatct tcaattaaaa caacattatg
     1501 ttctttgcat attttagtaa tttcatctag ttcagctgat aaaccgtaca aatgcactac
     1561 taatacagct tttgcgttag gatatttttc tagcgctgtt cgcaaatgtt cagggttcat
     1621 attccaagtt tcgtaatcac tatcaacaaa tactggaata gctccttgat agatgattgg
     1681 attggcagta gctgagaacg tcaaagactg acaaatcact atatcatctt taccgacccc
     1741 agccgctttt aaagctagat gtatagctgc tgttccagaa gatagtgcag ctgcatgact
     1801 agcaccgaca tattctgcta attcctcttc aaaaccattg acattcatac ccaaaggagc
     1861 cacccaattt gtatcaaatg cttcttttac atactcttgt tcatatcctt catcactcat
     1921 atgaggagat gctaataata ttcgtttatt ttccaactta atcccatctt tctatgactt
     1981 tggtaggtac gccaacattt ttagtcttat tattaatatt ttttattact acacttcccg
     2041 cacctataat gttcatattt ccaattctta gattttgtat tattttagta cctactccaa
     2101 tttcattttt atctcctaat agaacatgtc ctgaaatatt tacacttgga aaaattgaat
     2161 tataattacc gatttgagta tcatgtccga tagtagagcc gatattgatc atattgaaat
     2221 ttcctaaaac tacgtcagca gtgtaagtcg tatagggcat caaaatattg ccaattccta
     2281 actgaacatt tatgcccact agtgcacttg atgcaactaa attagggaaa atcaatttat
     2341 tatttttatt tatcttttga tatattttct ctttgatatc tggcgaagcg attcccagaa
     2401 aaatagcagt ttcttcttct aattctaata acgagtctat tgttcccaat acagtttttc
     2461 ccactaattc ttgattttgc atttttaacg aatcatcgac aaaaccaatc aattcatatc
     2521 tagagaaact ttctagtaga aaagcaatct ctttgccaaa tccaccagct cctacaataa
     2581 ttgctttcac tttactcccc ctacttattc gaagcattcg caaaagtgac aatgtcttta
     2641 gccaattgtt catcgtcttg cggcaggctc ttgacaaatt ccatcactgt ttgcatggaa
     2701 taacctttga tgtttcccac aaagatcttc tcgtaaactg cttcatcgtt tctttcctta
     2761 tctaacaata gttcttcata tagtttttcc cctggacgga tacctgtttc aatgatttcg
     2821 atctcgtctt cgctatatcc gctcaatcgg atcatgtttt ttgctagatc tacgattttc
     2881 actggttcac tcatatctaa aataaagatt tcgccgcctt ttgctaacgc cccagattga
     2941 ataactaaac gacttgcttc aggaatcgtc atgaaatatc gggtcatacg aaagtctgtt
     3001 acagtgactg gccctccttg agcaatttgt tcccggaata ctggaatcac actaccgcga
     3061 gaaccaagga cattcccaaa tctgacagct gagaatttcg tacatccttc ttcattcaac
     3121 ccagtcacga tcatttcagc gattcgtttt gttgaaccca tgacgtttgg cggattgttg
     3181 gctttatctg tcgagaccat gacgaagttt tttactttcg ctgctttggc tgcttctgct
     3241 acattttttg taccgaaaat attgtttttg actgcttctt ttgggttata ttccatcaat
     3301 ggtacatgct tatgagcggc tgcatggtag acgatatctg ggtggtattg ttccatgatc
     3361 tcattgattt tcttgcgatc ttggatatcc gcaatgatcg gcacgatttc tgtaggacac
     3421 ttttgatgat gcgtgcgtaa ttcacggtca atcaagtaaa tcgagttctc cccatgacct
     3481 aaaagtaata gttttgcagg gtcaaattgg atgatttgcc ggcagatttc tgaaccgatt
     3541 gatcctcctg caccggtgac aagaatgact ttgtttgtga tctggtcttt gatggattcg
     3601 atatccaact ctacctcatc tcttcccagt aattccacta cgtcgatggt ttttaatttt
     3661 gatacgctga ttttccctga ggcaagttct tccatagaag gcatcgtatt gactttcaca
     3721 tgagcagatt cgactaactc gaagattctt cgtaattttt ttcttgataa agaagggata
     3781 gcgatcgtta ccatttggat gtcatatttt tcgatcaatt ccggtatatc ttttaatgac
     3841 cccagtactt tttttcctga aagataggta tttcttttat tcggatcatc atccacgaat
     3901 ccaatgacat ggatatcttg agccgtttta gaaccaagaa agctgttgta taaaatacgt
     3961 ccaccttcgc cggccccaac gatcaaggta tttttagcgg aatctgttga tagatacctc
     4021 atattttttg tttcgatata gatacgccag atcaatcggc tgccaatgat cagtaatagc
     4081 gaaagtaggt atgcaaagat cactaaacgt aaactgtatt gtttatttat gaaaagcaat
     4141 atgatgatac ttgaggccgc acttgcagat aacgaaccaa aaatcgcaat gatctctctt
     4201 aaatttgtat aacgattgat tcgagtaaaa actttgaata aactaccata gaataaataa
     4261 aaaccaatcg acaaaccgat agagattaaa ataaggtcca ttggtatagc tacaaacggc
     4321 ttcatgaatt tagctgctgc aatattcgca gcaatcaaca acaagctgtc cacaaatacc
     4381 aatgtggcaa tttttcttct tcttgttaat acaaacaccc tacattcccc ctaaaataaa
     4441 ccaaacttct tcttttttac ctcttcatag actggataac tgatctcatc cccgttgatc
     4501 aggtcctttg cgacttgttt catctgtgct actttttcct taccaaagtc cttggcgatt
     4561 tgctcataac attcttttaa gtaaaaggta cgtttagaga ccccatgggc atcggaagca
     4621 gccatttgca cgaggttatg tttgaccatc attttggctg ttttctggat gtcttttcca
     4681 aatacaccga cataacttgg tgcggtgagt tgagccaagc atcccatatc taggaatggc
     4741 agcaaatgat ttgggtcttc tcgaaaatgc gcgttgcgtt ctggatggac gatcactggt
     4801 gtctttccta gttgtaacaa ctcaaaaaag agccattctg catataacgg cacttccagt
     4861 gtcggaaatt cgattagaag atatgtatca tctaaatctg taaataaaat ttcatctcgg
     4921 tggatgtctt caatcagtgt tccggtaata cgtacttctt gtccttccaa taacgtcaaa
     4981 ggcaattgtc ttttctctaa ttctgcttgt aacgaagcga ctagagaaat gacttgtgat
     5041 ttttcattgc tgtattttcc attattgtga tggggtgtgc ataaaatatg tgtgatccct
     5101 tgttgaatcg ctttttcagc catggcgata ctggcatcta tgttttctgc tccgtcatcg
     5161 attcctggca ggatatggca atgtagatcg atcatttctc ttgtctcctc tctttttatt
     5221 tcttaattcc cgtagtaata gccagcatct ctgctgtgtt ctgctccatt gtatacaaca
     5281 ccaaggactc gcgcttgtac catatctaac agttcttttg ctttagtcaa tgattctttt
     5341 cgtgacgtat tttcccgtac gaccaataag gtgccatccg tttttgaaga catgatctgt
     5401 gcatctgtta cggcaacgac tggtggcatg tcgaaaatca ctacatcaaa tagttgtctt
     5461 gcttctgcta aaatctgatc cattcttggc gaacttagta gttctgatgg atttggtggt
     5521 ttaggtccac ttggcagaat cgacaaattg tcgactggtg ttcgttggat ctcatctgct
     5581 acacttcctg atgaactaag aacagtactt aatccgcttg cgttgttcaa cttgaacgtt
     5641 ttatagatga cgggcttacg taagtctgca tcgactagca gtacccgttg accagatttg
     5701 gcaaaaacaa cggcaatgtt tgctgcagtg gtggattttc cttctccagg acctgatgaa
     5761 gtcacgacaa tcgttttgat ttgctgacca gctgcggagg caaactggat attcgtccgg
     5821 atcgtacgat attgttcggc gattggtgaa gatgggtttg ttaatgtgat caaactgact
     5881 gcatgagttt gagttgcttt ttgtttctgt gttcgtgcca tttttcttcc tcctagactc
     5941 ttgagcggct tctgcgtgat ggggctgttt tatcgtttgt agcttttggt cgtgcaggag
     6001 ctgatccttt tttacggata ccagcgttca attcttttgc tgacatttct ggaactgttc
     6061 ctaagattgg gaatcctaat gtatccgtca catatttttc atctttcact gtccgatcaa
     6121 gtaattcgaa taagaatgcc agacctacgc ctaccatcat tcctaacact aagccaatca
     6181 ctagattcaa tttattattt ggcgaaacag gtgtcgtatt ggcaactgca cctgaaataa
     6241 tcgagatttt atcgacattc atcacgtctt tggcgttttc ttggaaaact tgtgaagttg
     6301 tattagcgat ctgttgagca gtcactgcat tcgtgcttat tgcctggatc gagaacattt
     6361 gtgaattttg tgactgattg acggagatcg catctttgat ttgtccagca gtcattttca
     6421 cattgtcttc tgtttctaaa cgatctttga cttcatttag tactaaatca ctgacgatca
     6481 tatctttata cgtattgatc atttgcaggt tcgtattgac atcattgaca tttgctgtgt
     6541 ctgattgtgg cagtgtcacg atcaattgtg cttgtgaact gtattttggt gtcaagatga
     6601 aaaatgttac gattcctgag atggccaagc cagcaaacat tgagatgatg attgttacca
     6661 agtgtttgcg taatgtttca aaaatttctt tcaagctgat tgtctcttcc attttttctc
     6721 cactttccta tttattctct agattcattt gatcttttaa ttcttttttc acacgatcaa
     6781 gttcttgatc gctgactttt tgataggaga tattatcttg catgaagcct tctccttgca
     6841 gctgatcttg cttgatcgta ccaaatgcct cccgataatc taaagcgatt tttttcattt
     6901 catcaaagct catgtctgtt ttcatattcg actctagagc attcaaaacg gattgatagt
     6961 tgctgacacc gtttaaactc aatacttttt ttgctacacc ttctacgatc tttctttgac
     7021 gctcttgtct accatagtca ccatttggat cttcataacg catacgagaa taagataatg
     7081 cctgatcccc gttcaatgta attttaccga ttggaaagtc atagccatct tgtgagaaag
     7141 tgaggtcatt attgacttcg ataccaccaa ctgcatcaac gagttccttt agccctttca
     7201 tattgataga gacatagtga tcgattggga tgtctacata tttttgtagg gtatccatcg
     7261 ccatggcagc tccgccgaat gcgtaagcgt ggttgatctt gtcttttgtg tcacgaccta
     7321 cgatgtcgac atacgtatct cgaggaatgc tgacgatcgt tgtctgtttg tcttcaggac
     7381 tgactgtggc aaccatcatc gtatctgatc ttccttgatc gacccgacct aaatctccag
     7441 tatcaatgcc catcaataga acagagaaag attcttgttt gtccagatca actggtttcg
     7501 ttcgttctcc ttgggttcgt tccactgatt cgtaagtttt ctcaatagaa tgtgagagat
     7561 ccagatataa ttttgctcct actcctacaa ttgctaatac taatacgata agaactgcta
     7621 aaataatcag aaatattttt ttccctcttg attttcttct tcgtcttgcc atagtttcct
     7681 ccgtttgcta accttgcttg aatcaacgtt ttaactgtca ataacttata ttgctatagc
     7741 ccatttgctg agcaacagtt tgtgcttttg ttgcgtatgc tgcgcttatt ttttcattcc
     7801 ggatctgctc tatttgactg agcgccgtca aatattgatc gtaagtagcg gtggcagtca
     7861 cttgaccatc cttcaacatc gtatcgaata actgatctag ttgcgtgact tgcgcgatct
     7921 gagtatcagc gaatcctaga tattgcataa caaccgtttt ccaaggacta tctttaaaaa
     7981 agctcatatt ttcacgtaca cgagcgacat ctgcacttgt caccttttct ttgatgacga
     8041 catcgtctac agcttgctgc cagtttgata catttttagt aaataaatcg ttgacagctg
     8101 tctggatctt cagttttgat tctgcactat ctaactgtgt cacgattgct ttcttctcat
     8161 cttggattgc aacagcttct ttaggcaatt ctgattcttt gatgtcgtaa tcatctgctg
     8221 aaagtttgac agaggatagc ttatttgaca aacttgtgat tgtttcttct gttatttcag
     8281 ggtttagaaa atttttttct tttgtactat acagcttgtc tacttctttt gataaaggct
     8341 ctataatttc taggactgat agaaaatcat tctatcggtc ccttttgagc atgaaaaaac
     8401 aaaacataca gaagctccgt tagaattgaa tcaccacaac acaatcaaag gagcgttctg
     8461 tatgcctgat cttcatcata tcaaactact acttggaatc aaagataaaa acattttcat
     8521 cacaaatgtg gaatcgaaga gcattaagaa tatcaagagt cttgttgttt ctgcgacgtt
     8581 aagtaaagaa attcgtcgct gtcctttgtg taaacagatg aatcatgaag gcatgatcgt
     8641 taagaacgga aagaaaaaat cgctcattca acttaataaa tgtgcgaacc aactgactta
     8701 tcttgcttta gcgaaacaac gctaccactg tcgtggctgc cacacctatt ttacggcgaa
     8761 tacctacatt gtcgatagaa actgctttat cgctaaacaa gtgcgctata aaattttaga
     8821 agagctaact gaaaaacaag caatgacgac aattgctaag cattgcggcg tttcttggtc
     8881 gaccgtttct cggacactcg cttctctgat ccccatgaca aaagtaaaaa gaaattggtt
     8941 accgcgttgt ttattaatgg atgaatttcg ttcactgaaa aatcaagtcg gtccctactc
     9001 cttttcttgt atggatggcg atactggaaa attactggat attcttcctt cacggaagaa
     9061 aaaggctctc gtttcttatt ttatgcaatt tgaacgacgc gcacgattga aggtaaaggt
     9121 catcgtgacg gacatgaatg tttcttatgc ctctctgata aaggaatgtt ttccaaaggc
     9181 aaaactcgtt gtggatcgtt ttcacattgt caaacatctc attcgtaaat ttgaggatat
     9241 tcgcgtacga atcatgaaaa gctttgatcg aaacgatcca acacaagcca aatattatcg
     9301 acaattaaag gcgttgtctc ggcttctgat caaacgacag gacaagcttg tttatgacaa
     9361 atggaccaaa tggcgcaatt ttggttgggc gtatctcacc gaaagtgaag tggtggatcg
     9421 tctgctttcg atctctgacg aattacgaat cgcctatttt tactatcaag aaattcttca
     9481 agcgtttcac gacaaagagg cggacacttt ctttaaactt gtaagaacga tgcccaattc
     9541 cgttccaaaa gaactgcatt ctataaaaaa agcttttata aactatgaat ccggcattcg
     9601 tttagccctc gaattgcctt attcaaatgc caaaatagaa aatctgcata cccacatcaa
     9661 agcattgaaa cgagtcgctt atggctttcg aagctttcgg aaaatgaaga ccagaatttt
     9721 cttactaaat aatctgatta cctatgaatc aaaaaacatc taaagtagaa aaggaattct
     9781 accttagatg ttcttatctc actctatcag tcctatttga caaagagcct tgataaagaa
     9841 gtcaaggtgt ctttttgatt ggtgatggca gattctgcat aattgatccg ttgctgatgt
     9901 tgccgctgat tattgatatg gtaaccaaac aaacagatcg caatagcacc tgcacctacc
     9961 gccacagcag tcaatgactt gacgaatggc ttttccatag attcactcct aagtattgat
    10021 gatagataaa ttaagatata attcacatac ataaaaatga cagcttttt
//
LOCUS       DAXWLG010000094         9823 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXWLG010000094 DAXWLG010000000
VERSION     DAXWLG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.095"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(35..241)
                     /locus_tag="ACGE4T_001859"
     CDS             complement(35..241)
                     /locus_tag="ACGE4T_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387106.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(257..871)
                     /locus_tag="ACGE4T_001860"
     CDS             complement(257..871)
                     /locus_tag="ACGE4T_001860"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFF8387107.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1018..1701)
                     /locus_tag="ACGE4T_001861"
     CDS             complement(1018..1701)
                     /locus_tag="ACGE4T_001861"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8387108.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1810..2268)
                     /locus_tag="ACGE4T_001862"
     CDS             complement(1810..2268)
                     /locus_tag="ACGE4T_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFF8387109.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2352..4115)
                     /locus_tag="ACGE4T_001863"
     CDS             complement(2352..4115)
                     /locus_tag="ACGE4T_001863"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFF8387110.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4336..5796)
                     /locus_tag="ACGE4T_001864"
     CDS             complement(4336..5796)
                     /locus_tag="ACGE4T_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387111.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6004..6930)
                     /gene="glsA"
                     /locus_tag="ACGE4T_001865"
     CDS             complement(6004..6930)
                     /gene="glsA"
                     /locus_tag="ACGE4T_001865"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFF8387112.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6953..8380)
                     /locus_tag="ACGE4T_001866"
     CDS             complement(6953..8380)
                     /locus_tag="ACGE4T_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8387113.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8411..9625)
                     /locus_tag="ACGE4T_001867"
     CDS             complement(8411..9625)
                     /locus_tag="ACGE4T_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFF8387114.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2912 a         2041 c         1627 g         3243 t
ORIGIN      
        1 ttttatatgt tttttcactt ttcggaataa atatttattt gttaaagtaa ttaaacaatc
       61 cgctaatcgt ttgatctggc atttctattt ccttaccatc taaagtaaca gtagtaacga
      121 caaaatggtc ttgcaatggc ggaacttcta agtactgccc ttgcagaaaa tcattcactg
      181 tttcatattc ttttttcatt tcttttgaat ctgtcgatgg tttatagctt atttttaaca
      241 taactagtgc tcctttttat tcgggacgtg caatcgtgaa aatttctcct attgctgcat
      301 aatcatttcc tgccaaacgg cttacagcat tcaattttct tggatcgatc cttcctgtct
      361 tatcatcata gactgtttca tttaaatgat atttctgtac ttctagcaaa aataaatcag
      421 ctgttacttt atttttattc cgtatctcga catgttcata taaggatgtt tcaaaacgaa
      481 cttttgcttc tgtcaatgct ggtactgaaa tgattactga atcagttaaa gtcaagtcag
      541 tcaggtacag ttcgctttcc tcattaggca gattggctgc tgttttattt acttcttcta
      601 cgttgtcctc atcaaggata tggataacag cttctcttgt ctcaagaata ttctttgctg
      661 tgtcttttac tgcatcttgt tttctctgga tcgccaagga taagatgggt cgattcgaac
      721 tgacgatatt gaagtaactg aagggtgcga tattcactat atcttttgct gatttagtcg
      781 ttactactgc tactggtcta gggatgactg aaccaatcag caacttgtaa ttttcacgtt
      841 ctgtcaattc tgaaggactc cgttcaaaca tggtttcgcc ttctttctat aaaattcgtt
      901 aaaaagcaga tcttgaagac aaaatgaaaa gtaatgcttt tttcatcttc ttagaatctg
      961 ctttaaacta tctatcaatt tttctttttt gtcaagaatc tgtgccgtgc gtgtttttta
     1021 gttatttgct ttacttgcgg cttcgacata tcttttagtg atcaattgcg gacgggtgat
     1081 aatagatcca actacgactg aaaaagcacc taattcatat gctttctcta cttgttcagg
     1141 agagtcagta tgtccttcca taattactgg gatatcgact tgttctatga tttgattcaa
     1201 taaatcaaaa tcaggtgtct gcgtctttcg tgaatattct gtatagcctg ctaaagtcgt
     1261 agacaaaaga tcaaaaccat cttcttgcgc ttgaagcgct tcctcaaaag tcgaaatatc
     1321 tgccatagct aaccgaccgt gttcgtgaat catttggaca agttcagcag ttgttttttc
     1381 tgtcggacgt ttacgagaag ttgcatccag tgcaatgatt tctgcttgcg tctccaaaag
     1441 ttcttccact tctttttctg tcgcagtaat aaacacttct gacccatcgt aatctctttt
     1501 gataatccct attaccggta aatcaacgat tcgcttgatt tcattgatat cttctgtcgt
     1561 atttgctcga ataccaactg ctccgccttc ttttgcagcc aaagccattc tccccataat
     1621 aaaagaactg tgtagcggct cgttttccaa agcttgacaa gaaacgacga ctccgccttt
     1681 gatttgttcc agtgtatcca caatatctcc tccagtaatg ttatataaaa atacagcttc
     1741 atataaaaat gtaacccttt tctattcagg agacaataat gagctatttc tttcagcaga
     1801 cctttcttac taaaagaaaa ttacttcaaa ttcttcgtat acgaccagct tctcttctgt
     1861 aaattctatg ttcaacgctt ctttataagc atgtttaaaa aactcagcat ggactttccg
     1921 ccaataagct aatgtccgat ccccttctcc ttctttccaa gcatgctctt tcgtgacttc
     1981 actaaaaggc aagatggtca catttatatt tttgatgata caaactggtt cattttttcc
     2041 attcaggacc acgtctaatt gaccaacttt tggtaaaggc tcattctctt gtttatacag
     2101 atcgtaacca cttgatgtac cagtctttat cttttttaca actagttcgc tcaactcgtc
     2161 tgccattttt tctgtatccc caaatgaaaa gaaacctaat ttttctgatt gataactagg
     2221 atatttttct tggaactttt tgacatattc ttctacttgc tgatacataa catccacccc
     2281 tttgcttttt ataatgaaaa aaccatccag atactagtat aaacaattat ctggatggtg
     2341 agcataatga attattgaat attcaagaca cgcagactat cttcaacgat ttgttttggt
     2401 gttaaatgat aacgttcata aagctgccct acaggaacgc tatccgtgaa ttctttctct
     2461 gcaccaaaat ttaatacgtt cattgaagaa tcaccataat aacgactgat attttcgcca
     2521 aatcctcctg acaaactacc atcttctaat gtgatgacta attcgtgtgt ttcttttaat
     2581 ttatctaatg tttctacgtc attttctgtg atcgttcctg gattgatcaa cgttgcttcg
     2641 attccttttt ttctaaaagc ttttactgtt tcttttccta attcaagaaa accgcctaaa
     2701 gctaaaattg ctacttttcc tccattattt actgtttgat aggctggaat actataatca
     2761 tttgcgatag ctgtaccatg ttctacacca tgtgtaggaa ttcggattgc tacaggctgc
     2821 gctgtttgtt cgaacgccca atctagcata gagatcatat cctcttttgt tgctggagca
     2881 agatatttga tgttcgggat acttgataag taaggaatgt caaatgttcc ttggtgtgtt
     2941 cggtcactag ctgagatcgt tccgccaccg actaaaataa tcactggatt ctcgttgatc
     3001 gctaaatcat gtgataattg atcatatgcc cgttgcaaga aagtacttga ttggaagacg
     3061 actggacgta ctccctgaga agccaaagca gaagcgaaag tgatagagtg ctgttcagcg
     3121 atccccacgt caaaataatg atctggatgt ttttcttgga atgttttcag tccaaaggcg
     3181 cctggtatcg ctgcattgac tgcggcaata ggagctgtac ttgtttgcat tttcttatcc
     3241 aaataatcca tgatgatcat attaaatgtt tctccagaag ttacttgctt tgcctccccg
     3301 gtggacagat caaatggtac atgccaatga tatttcattt tattatcgat tgccggctga
     3361 taaccgcgac ctttttccgt atggacgtga agtacaatcg gatgatcaat atcttttact
     3421 tcttcaaata aacggatcac cgtttccaga tcgtttccat tatccacgta acgataatca
     3481 aatcccattg ctttaaatag gttattgctg cttttaccgt tggtttgacg aagctcagct
     3541 aagtttttat ataaactgcc atgattctca tcaatagaca tctcattatc gtttactaat
     3601 acgattagat tggattgtaa aacaccacca ttgttcaatc cttcaaaagc taagccacca
     3661 cttagggagc cgtcaccgat aaatgctacc acatttcctg attctttctt cagatctctc
     3721 gctttagcta aacctgttgc taagctgata gatgtagatg tgtgccccac tgtaaaatag
     3781 tcatgtttac tttcatgttg gttggaatat ccgctcactt cacggtaatg tcctttttca
     3841 aaaccatttt ttcgaccagt taaaagctta tgaccataag actgatggga aacatcccaa
     3901 acgattttat cctttggtga atcaaataca taatgaaacg caatcgttaa ttctactaca
     3961 cctagattcg gaccaacgtg cccacctaca tcgctgtcct tttgcaagat tttttggcgc
     4021 atctcttctg ccaactgttt catttcctca acagataatt ttttgacatc tgcggggcta
     4081 ttgattgcat ccaatacatg taatactgta ctcataatac attctctcct ccgtactatc
     4141 ttcttttaat gaaaatagat ttgttacact ttatctattc tactacttag agttgactct
     4201 aagtcaatag cattttaaaa aaatttcaga gaaagcttgt tatataagta gatcttcgca
     4261 cgaatcagga ctcaaataaa aaccttctac agacaattaa aaagctttgt agaaggtctc
     4321 ctaattaagg tctcatcaag aaatatgttt ttttaatagg tgaagttcct gagcaatatg
     4381 atacaaatgg atgtttgcac cttttaatga gattggatag atctttgtct tttcagtttc
     4441 taatccattt tgatagctag cagcatcgca caaaaccact ctttttttcg cacatagaca
     4501 ttcatcaggc aattcttcta atgaacgaaa taaaatagga tttatttcag cgatgccttc
     4561 ttccgcatac aaatgaaatt gggttcgata aaaaaattgt ttgactgaac tttctgaaac
     4621 aacaaaaatt tctatttcct gtctccccgc ttgttcactg ttgatcaaag aaagactttt
     4681 gacgaatttt cgaatgaaat tttcattcca tcttagttgg atttgatttt tctttttcca
     4741 ctcttccaat atttctacaa tttttccgta cagaagttgt tctttttctg atagaagata
     4801 gatcctttcc ggtaaaaata cttgtccatc tgcccacaac gtacggacaa attgtaaaaa
     4861 ggattgcttg aaaataaaat tttctgcatc gtcgatttca gcgatactaa taatctgtaa
     4921 aatcaattca ttcagtgata ggtcattgag aacaaaattt ttataaacaa cttcgaaatc
     4981 ttttcttaac agctccatgt tctcattggt ataatttcta ctgttgaaca aagaaaaaat
     5041 ataatagaca tcttcttctt gtaagtctag aattcctttt tgctcttcca atccttgaca
     5101 gactaattga aataacggct tcgtttctgc gcttttctta tcttcttctt tcagtccaag
     5161 gggaaatgcc tttcttccga ttgcgatctc tattcctcgt atgataaagg acttttcatc
     5221 ttccgaatat cttcttttaa aaaaatgagt ctctacataa tcaatcaact cgttacaagc
     5281 ttcttctaca tacggatttt tattttcata gccttcccaa ttgacataac gtactaaagc
     5341 tagcaagatg ttccgcgagt caaattctgg gataatgatt tctttatttt tcaaataatc
     5401 attttcttta aaaaaatcca atacgatttt tttgattgta tatactttgc tcaaagaaat
     5461 aaattcaaga tcagccagtt ctgtactcgt gaatatacca ttaaaataat aacttaaaaa
     5521 ctttaaaaaa taagattgac cgtacagaaa ctgtgtgagc tcagaaagag caataccatg
     5581 tttgaatacg agtttatact tatgcttctc tcttacttgt tcttttatac aatattctag
     5641 acactcggct agactttcta gatcgtgaag gatcgttggt gtagtcaccc ttagagaaat
     5701 agctgtttgt tcgatgtcta tttcttggaa ttcaaataat tgttcacaca gatacacttt
     5761 tctaaatata tttcgctcga tgtaattttc cagcattcct gatacacgcc tccagttttt
     5821 cttatgattt tatttaatca tatctgtaaa tcgtattttt gtttcctttt tatttagaaa
     5881 agccctttca aaaaccaata aaaaaagaaa ctgggacaaa agtcaaacaa gctcacaatt
     5941 tcagcctcat tgtaggtagc tttgagccgt ttggactttt accccagcct ctttcaagaa
     6001 tggttattca aagatgtcag cgtctagttc tttgactaca tctttcaaca attggatacc
     6061 agctgcgctg ttaccaaatg gatctagtgc cgggctgaag acacctatgc cgtaacgatt
     6121 tggcacagct gccatcaacc cgccgcctac accgcttttt gcaggtacac caacatggac
     6181 ggaaaactct cctgattcat catacaaacc agcagttgtc ataattgatt tgacgattgt
     6241 agcactttct tcagtaatga tttgtttgcc attccaagga gcaattcctt tatttgccag
     6301 cacagcagct aattttgcta tgcctgtagc ggtgacattg agggagcatt gtctgaaata
     6361 agcatcgagt atctccggta cgtctccttc gatcatttga ttacctttca tatagtaagc
     6421 aagagagcga ttgatatcac ctgttcttga ttcagaatga tagatttctt cattcaatgt
     6481 gatattagga tcatcacaga tctctttcat aaatgacaag attcgctcaa atttttcatc
     6541 tgcatcttta ccggcaatca acgaggttgt tgcaatcgct cctgcattaa caaaaggatt
     6601 cattggatgt ttgcgatgat tgatttccat attcatgata gaattgaagg caaaaccggt
     6661 tggttccgtg ttgattgttt gaaagacttt ttctattccg ttgtctttga ttgctaataa
     6721 caatacggga acttttgaca tgctttcgat ggcaaagcgc acatcggcgt ctcctccctc
     6781 aatcagttgt tttgtatcca aatcataaat cgccatacct aattgtttgg gatccacttc
     6841 agctaatgct ggaatgtaat ccgccacttg tcctgtttga taatagtgga aattttttcc
     6901 aatcaattgt tctaattctt ttacttccat ttgaataacc agctttcatt ttttattttt
     6961 ctgcatgctg gaatttatga cggcatgcgt agatcgtaaa tggaataact aagacgataa
     7021 gataagaaac aatcagaatc gtttcataaa tcccatgctg tccagctgcg atactagcag
     7081 gtggaacaaa tgagatacaa aaagcaaaaa gtgaagtcaa aagacctaaa caagcaacga
     7141 ttacttttac aacttttccg cctggaacct gataggctct ttcaagactg tcatctttaa
     7201 aaaccagctt caaatagccc gcgaaaaaca taaagtacaa catcaggtag attacaacag
     7261 tcaatgacat cgctgcaaga aatgaaagat tattcccccc gccgcttaat gtaaagacgg
     7321 ctgcccagat tgttgcaacg atcccatttg cgatcatcaa atgagcagga acaccgtgtg
     7381 tgttttcttt tgcaaaatat tcaggaagta atccttcttc tgctgctacg tgaagtgctt
     7441 ttgaaggaga ggttacccaa gaagcaattt ctccgatgac acctaaacaa acaagaatcg
     7501 caagaatttt cgcaatccat gcaagatttg gatcaatgtg tcttaatagg aattctaacg
     7561 attgaatcac ccctgtatta agagacaaac catgttgagg tactgtagta gcgacggtca
     7621 atccgccaaa agtatctaaa atgattgcca gaataaccaa tacaaacatt gctaatggat
     7681 aatccttttt agggttttcc atttcatttg catgactggc agatgtttct acgcccatat
     7741 atgctaaaat aaaggaaaca aaaacaacca gcgtaggcaa tttcgaaaaa tcaggaatca
     7801 atgttttcat agacagtatc gtttgtagtg gagcgcctga agcgacataa tggatcccta
     7861 agccaaacaa gactaatgct ggcaacaaga taccagctac aaaaccgatc cgagcaatca
     7921 atgaagtgta tttggttcct tttaactgtg agagtaaaat agcccagaaa ataatcaata
     7981 ggattgccaa tttcagatat ttattgtttt gtatagcacc aatacccgtt gcataggata
     8041 atgcaccagt taagaaatac agcatggtca agtagccaac tgttatctca aaccattgga
     8101 aaaagattgc ggcaaaaccg aatcgttttc ccaatgtgcg actgacccaa gtatagacac
     8161 cgcctttttc ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa
     8221 accatagcaa accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg
     8281 tgggatattc atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg
     8341 ttaatttttt tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt
     8401 tgaatttgtt tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt
     8461 cttcttttgt agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca
     8521 gcacataggc atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc
     8581 agctgcttgg attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac
     8641 ctgtgaatcc gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct
     8701 ttttacgaaa atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg
     8761 cactttttgc ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc
     8821 ctgttagtac gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag
     8881 taatcatcgc aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat
     8941 ctgctcgaag tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac
     9001 cgatagcagc tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt
     9061 gaccaagtgt gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc
     9121 caccagcgaa atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc
     9181 aaacagggaa ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat
     9241 tcaatctccc tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt
     9301 ggtaaagaaa gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg
     9361 taacatgtct catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc
     9421 caaaggccaa tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa
     9481 aagattgaga catgatcgtt agtatattat tcttccgtgc taatccgccg taaaaaaaag
     9541 ctagaccagg agtcatcagg caaaccattg ctgtacataa aatcatgaat gctgtatctc
     9601 ctgtattgat aggtaatgta ttcatttttt tcgcctcctt tctacgatta cgagtataaa
     9661 agaaggtgta gttgtttttt tcttctgttt ttttgaatta atacttgcat ttataaaaga
     9721 cagacaatta ataaaagctt gatgtgttgt gctctttttc gtttttcaca caaataaaga
     9781 caaaaaataa acggtcttct ttttatagaa aaccgtttat ttt
//
LOCUS       DAXWLG010000095         9775 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXWLG010000095 DAXWLG010000000
VERSION     DAXWLG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.096"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            92..372
                     /locus_tag="ACGE4T_001868"
                     /pseudo
     CDS             92..372
                     /locus_tag="ACGE4T_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(450..983)
                     /locus_tag="ACGE4T_001869"
     CDS             complement(450..983)
                     /locus_tag="ACGE4T_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFF8387115.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1077..2255)
                     /gene="mutY"
                     /locus_tag="ACGE4T_001870"
     CDS             complement(1077..2255)
                     /gene="mutY"
                     /locus_tag="ACGE4T_001870"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFF8387116.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2248..3045)
                     /gene="recX"
                     /locus_tag="ACGE4T_001871"
     CDS             complement(2248..3045)
                     /gene="recX"
                     /locus_tag="ACGE4T_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFF8387117.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3152..4534
                     /gene="rlmD"
                     /locus_tag="ACGE4T_001872"
     CDS             3152..4534
                     /gene="rlmD"
                     /locus_tag="ACGE4T_001872"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8387118.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4708..5286)
                     /locus_tag="ACGE4T_001873"
     CDS             complement(4708..5286)
                     /locus_tag="ACGE4T_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFF8387119.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5608..6078
                     /locus_tag="ACGE4T_001874"
     CDS             5608..6078
                     /locus_tag="ACGE4T_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFF8387120.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6186..7412)
                     /locus_tag="ACGE4T_001875"
     CDS             complement(6186..7412)
                     /locus_tag="ACGE4T_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8387121.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8166..8366)
                     /locus_tag="ACGE4T_001876"
     CDS             complement(8166..8366)
                     /locus_tag="ACGE4T_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8387122.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8500..9108)
                     /locus_tag="ACGE4T_001877"
     CDS             complement(8500..9108)
                     /locus_tag="ACGE4T_001877"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFF8387123.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9193..9663)
                     /locus_tag="ACGE4T_001878"
     CDS             complement(9193..9663)
                     /locus_tag="ACGE4T_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFF8387124.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         2899 a         1957 c         1608 g         3311 t
ORIGIN      
        1 tcttttattc tttttttatt tttgaaaact tgccatactt tttatttgtt ttattttcca
       61 gtatactata cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat
      121 gagcaatatt tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg
      181 tatgcctttg tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta
      241 ctcagataaa aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat
      301 gaaattacag cggatggcga tccttttgaa gatgctggca tgcctcatat ttatatgaaa
      361 aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca gcaagctttt gagaaaaatc
      421 tctcactggc ttactgttct tttttgtgtt tacttacgcg acaattgctt gtaacggtta
      481 taccaaatat caatatagcc ttccgagaac gggccatctc cattattgat ccagtcaacc
      541 agaatcttca cattttcttt taggatgaag tcaatatcgt tcggataatt catcattttg
      601 ctgtgcatct catattcatc gacatccaat agtcgtttct cgccgtcagg aaataccttg
      661 atatccaagt catagtcgat atatttcaag gcctcatcgt ccagcaaaaa aggtgaagct
      721 agattacaat aataggaaac ccccttttct ctgatcattg ctattacatt gaaccagtat
      781 tttttatgaa aataaacaat cgctggttcg cgcgttaccc atcggcgtcc atcagattca
      841 gtgactaatg tgtgatcatt cacgccgatc atagagtgtt cgcttgtttt taataccatg
      901 gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt aactttggat cgtcacaaac
      961 tctccttctt ttggaactcc catagcaacc cttccttaag cagcattttg tgctttgtgt
     1021 ttgggtgttc attacaccga catcataatt attatatcaa taaaaatgag ctttgcctag
     1081 aaaggattcc tattgtttcg atttttctct aactgatcta ccagtttcat ttggacctta
     1141 ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact tcgaagtttt gttctcggta
     1201 aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa ggagaacatg ccatttcaga
     1261 tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct gccaaacaaa gagttcctgc
     1321 ttttcaaatg ggagattttg ctcttcttct gctactaggt catcaaacag atcgatttct
     1381 ggctttgttt cccattcttt tttcaatcgc tcatactcct cttgtgtcac ttccatcatt
     1441 ggaaatgtcc acatattcgc cagcaatttt tggctatctc tttcttcaaa ataatacgct
     1501 ccagaatgat tttgaagagc cgcactgata taataaacgt cttttggctt tgcttttttt
     1561 gtttttacag gaaaactcgt ttggattcct cgtttgttag ctaaacaaaa ggcttggatc
     1621 ggacaagtct cacactttgg agaagttggt gtacagatag ctgaacctag atccatcatt
     1681 gcttggttga attcacctgg atgcttttca tcaatgattt tccgcatcgc ttcatcaaag
     1741 attttcctgc tagaagcttt tgcgatgtct gcttcaatgc aaaacaatct gctgactact
     1801 cgcatcacgt tgccgtcaac cgcgggttct ggaagtccaa atgcgatact tgcaatcgcg
     1861 cctgtcgtat atggtcctat tcccttcaat gagctaattt cttcaggcgt ttgcggcatt
     1921 tccccatcaa attctgacat gatctgttta gcagcagctt ggatgttccg ggctcttgag
     1981 taataaccaa ggccctccca tgcttttaaa agcttttctt ctggagcatt tgctagctct
     2041 tcaattgtag gaaaccattc catgaaacga taaaaataat cgatcactgt gtctactctg
     2101 gtttgctgaa gcatgatctc agagatccag atacgataag gatcacgatt atagcgccaa
     2161 ggcaggtttc ttttttcttg ttcataccat tgaatgaact gatcttgaaa ttcttttgtt
     2221 tctttatctg tccatttttc ccattcttta ttcactttcc tcttcctttt ccgtaatgaa
     2281 tcgctggatc atatccaaat caaatccttt ttgatataag ctttgcttta cttttagatt
     2341 tcttttttta ggttcaaacc ttaaatttct tcgccatagt ttatctcctt gtttgactaa
     2401 ataatcatat tctatttctt gatctgcttc ttgcggaaga tcagccatcg cttgttggat
     2461 cacttcttga gaaaaacctt tcgtcatcaa tgtttgttgg atttttctca ataattcttt
     2521 ttggcttttt ccgcgatttt ttccaaacgc tttttcggct gtctgatgtg cattttgaac
     2581 ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg atttcaggtt tcaaaccttt
     2641 ttgctgcatc tgctgcgcta atttctttgg tcctttgtca cttaagcgca tattcgtgcg
     2701 cacataactt tccccataga ccaaatcatc gacaaggtcc aattctttta agcgggctac
     2761 gattttattt cgatcttctt gaaggatttc cttttctttt aagtatgttc ggatttcttt
     2821 ttccgatcgc aactgataac ttaagtaatt cattgctagg tgaaaaccga aatcagctcc
     2881 gctactcttc ttgatctctt ggatagtttc ttcgtctaat tcccgtcctt ttaataatcg
     2941 atagcgtact aatacatctt cagatacttg taaagcttct ccttcagata aatctacgcg
     3001 atagaaaggg cctttgcctt tactgattct cgtaacggtt aacataaaca tcctcctcga
     3061 acatatattc tctatcatac cacagaaaat ccccgtaatc aggtattata tgttagaata
     3121 aaccacagaa aaaagaaaaa gggagatact tatggcagaa acaattgtca aaactggaca
     3181 aacggttcct ttgaaaatca agcgtttagg cataaatggt gaaggaatcg gttatttcaa
     3241 acggttgatc atatttgttc catatgcact accaaaagaa gaagtactag tgaaaatcac
     3301 gaaagcaact cctcgctatg cagaaggaga attaataaaa gtcaaaaaaa caagcaaaga
     3361 tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt ggctgtcaat tacaacactt
     3421 agcttatcaa gctcaattgg attttaaaaa ggatctgctt ttgcaagcat tggaaaaatt
     3481 caaaccagca ggctttcgat cttatcaact tttgccgacg atcgggatgg atgagccttg
     3541 gcattataga aacaaagcac aatttcagtt acgaaaaaat aagcagactc aaaaaattga
     3601 agctggttta tacgaagcaa actctcatca gttagtccct ataacagact gcttggttca
     3661 agaacctcga acacaaaaag taatgaatac tgttgttcaa ctattgaata aatttgatgc
     3721 acccatctat gatgaacgga caaacagtgg aatcttccgc acaatcatgg ttagaatcgg
     3781 tgttaaaact ggggagttac aagttgtttt tattactcgc agtaaaaaat tcccgcaaaa
     3841 aaacgcaatg atctgtgaga tcaatgaacg actgcccgaa gttgtttcaa tcatgcaaaa
     3901 tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa acgatccact tatggggaaa
     3961 agaagccatt gaagaacaaa tcaatgaagt gactttcgat ctttctcctc gtgccttctt
     4021 tcaactcaat cctcagcaga ccgaattgct ttatggcgaa ggaatcaaag cattagatgt
     4081 ccagcctaat gaaacgatcg tggatgctta ttgcggagtt gggacgatcg gcttaagtgt
     4141 agcaaaacaa gccaaagaag tacggggaat ggataccgtt cctcaagcaa tcgctgatgc
     4201 aaaaaaaaat gcggaaagat taggatatca caacactcga tatgaagtcg ggacagcaga
     4261 gcaagttctg cctaaatggt tgaatgaagg ttttcagcct gatggtatca tcgtagatcc
     4321 accacgtacg ggtttggata acgcattgat ccaatcatta ttgaaatacc ctccaaaaaa
     4381 acttgtttat atctcctgta atgtttctac acttgccaga gatctggtta atttaagtaa
     4441 aaagttcaag attgaatatt tacagtctgt cgacatgttt cctcagactg cacgttgtga
     4501 agttgtcgtg aagatgacga gactcgctga ctaaatgaaa gaggttgtga aaaagtctgc
     4561 cctgtatcaa gtaataagaa cagccaaaca aaaatagctc ttcatgtttt atgaataccg
     4621 tacttgtgac cgaagagtta acaggaggac actatttatt taaaaaaagg gttgtgacat
     4681 agtttttgtc acagcccttt ttgattatta ttggatattc acgtctatca cttgataaaa
     4741 tgcatttgtc gtatcgctga tattccatac tgctaaaatc acattatatc cttttcgatc
     4801 ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga acagttgctt tgtcgtctat
     4861 ttttgtgatc aattcaaaat cactaaattt caaaggttga ttaggattcc aagttggttt
     4921 ggtaatgtag taatcccaag tacttgtccg atgttgtgct gttaaattcc aagtaatatt
     4981 taatgttcct ggattaacca tgtttttatg ccatctgtta gatgtctgtt catctaaagg
     5041 ctcaaagccg ctgactcctg cactagcaat ttttccatca atgaatgtat ttttggtagc
     5101 ttcgatactt tgcggttccc attgtgctct tccgacattt tgatttaagt ttcctgctgc
     5161 tgatgttcct aggtatgcac ggcttcctgg agaagtaata taaccatgcg caaaacctgg
     5221 aatgctaaaa ccgaacaacc ctaaactaac taacccaata aacaataacg tactaatctt
     5281 tttcatgtag aaacctcctt catttgataa ccacattgta aacgctttat tgtattaaca
     5341 caaacattat gcttttataa ttttatgttg aagtaaaaaa ggtgaaaaaa gtaagatgca
     5401 gattataaca gaagtagacg agaaaagtac aaatagaaaa catgagaacc aaatcatatg
     5461 atctttttca aaaacatcat tcatataaac aaatgttcaa ctttttaata acaacaatca
     5521 tcaatttttt tcttacttca caaactaaat acaggtcttg ctgtgagcct tcatggtaaa
     5581 ctactattat agaaaggagt tagtaaaatg aagatgataa aagatattcg tttttgggtt
     5641 tgtgttatag gaattgtcat attaggcttt ttatcagggt tgcttagcgg aaatcccgga
     5701 gaatattact attctttgca acttccacca tttgcaccac ctagctggat atttgggccg
     5761 atgtggacct tactgtatat attgatgggt atttctcttt atttgttact taatcacaac
     5821 aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga ttcagttcat ttttaatttt
     5881 atatggtctg ctctattttt taatttaaga aatattttca ttgcagcaat cgatattaca
     5941 cttttagtta ttttcttgtc agtgcttatg tatcagttgt ggctacatca tcgcctggca
     6001 atgtggctca tgattcctta ttatttatgg gtactattcg caactctatt gaattattca
     6061 atttattttt taaactaagc gctaaaaaga gacgtcctgt aatttaacac atagaaaaag
     6121 tgccacctaa gttagatttt ttgtctaatt taggtggcac tctttattaa ccaaactatt
     6181 tttttctagt acccacttgt tgttccatac tcattattct ggtcataacc ataaccattt
     6241 tgttcctgtc cagcgtaagg atcataactt tgagtatctt gacttggtgt atagtactga
     6301 cttgtatctt ctgtttgctg ataataggta ttcggatctt ggtactgatc atatgtgttt
     6361 tctgaatagg aagaataatc ggtgtaccct tcactattcg atccatcacc gtttgtcgaa
     6421 tcgccatctt ccgaatcatc aaagaacatc attgaagcat tctcattttt caaattaggt
     6481 aatgtatcgc ttgtttttat atttaattgt ttcttcagtt cattttggat cgaaagaaga
     6541 tcattttttc ctaaaatttg atagtagata tcattgatca tttgtccttc gccttgcaac
     6601 tggtcttgtt tgattgtttc aaacgcaggg gtgtagttcg tcgctacatc aagcatgtca
     6661 tcccaatcta agtccgtcgt cacattgttc tcaactgctt ttaaaatttt tctataattg
     6721 cttacgccat ctaaacttaa aactttattg acgattttcg tcactacttc ccgctgacga
     6781 cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca ttcgagaata ggctaaacct
     6841 ttttctccat tcaatgtttg ttcacctact ggtacatgga caccatccaa agtaaagtca
     6901 attttattat tcactttgat gccaccgacc gcattgatca aatcttccat gccatccaaa
     6961 ttgatcgtta cataatgatc aataggaata tcgagcagtt gctcaattga atccatggcc
     7021 atttcgacac caccaaatgc ataggcatga tttaatttat ctaccgttcc ataacctacg
     7081 atattcgtat aaatatctcg gtccaaactg atgatcgttg attttttttg tttcggatta
     7141 actgttacta ccatcatcgt atcagaacgt ccttgttccg ttcttcctaa tccaccggta
     7201 tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat caatgcttac tctctcagaa
     7261 cgtttggagg atattcgatt agagttttta gaaattttgt ttactgtttg gctcgcttct
     7321 cccataaatc tgattccgta tgctgataat ccggcaatca gcaaaacaaa tacccctaac
     7381 aaggttaata gaactttttg ccatcgcttc ataatacacc tctgtttcac attttctaaa
     7441 atattgtctc atataactgt tcaatcagca aattcacttt ttaatcataa gaaaacttta
     7501 aaaaacattt ttgtgtaacg attactattt tttaaaattt ctcaaaattc ttaactatca
     7561 taccataccg tgaaaaagat cgaaagaccg aatacacgca gttcgcaatc gttttatctt
     7621 tttttacgta ttttttccag aaaaagcgaa caacaaggta ttttccaaat aatcgtgcag
     7681 taaaatgatc gcacatacct ttcccatctt tttaacagtc tttgcctacc gttcagatta
     7741 tcattcgatt tcacgaaaat ttcttcctat gactggtcct tttgttaaac ccatcttcta
     7801 ttcgttttat ttccagctca ctgcatggct cttaaaatga ctcgaactag tgatttcatt
     7861 accatcatct tttaatggat agatatgctc ctcttcaaag taaaacgatt ttcttttaaa
     7921 gtaatagaat tgacatggta atgagtatgt atactagtgg tttcacaaat ggtcttttat
     7981 tcaacatcca aaaactgaaa tttctttaaa atccattact aacgaagaaa atgttccttg
     8041 cttgataatt agttaggaca tgtcaagtga acaaatgcat ttacttagcc aaaaaatcta
     8101 aaaacttcat aaatagacaa aaagacacga ctctctaaga agtcgtgtct ttattcaaca
     8161 aattattata atttagtcac ttcagcagct tgaggtccgc gtgcaccttc aacaatcgtg
     8221 aattctactt cttgaccttc ttctaggctc ttgaagccgt ctccttgaat tgctgagaaa
     8281 tgaacaaata cgtcatctcc gccgtctact gtaataaacc caaatccttt ttcattgttg
     8341 aaccatttaa ccgttccgtg ttccataaat gtatagcctc caaaaaagtt cttgcaagta
     8401 accttgcaag aacattatac ccaatttaga aaacgctagc aagctttcgc tcttttattt
     8461 tccaaattat ttttaagact gatatgacgg aggaaatggt tattttgctg cttcgaaacg
     8521 tttgtttact tcatcccaat taataacatt ccagaatgct gcaatataat ctggtcggac
     8581 atttttatat ttcaaataat aagcatgttc ccaaacgtcc aaacctagaa ttggtgtctt
     8641 tccttccatc aatggagaat cttgatttgc agtagaggta atagctaatt ttccattttc
     8701 cattacaagc caagcccatc cagaaccaaa tcgtccagcg gctgcttttt tgaattcttc
     8761 tttaaaagaa gaaaaatcac caaaagcttc attaatcgct tcttttattt ctcctgtagg
     8821 ttcgccacca gcatttggtg ccatgatttc ccagaaaaat gaatggttag catgtccgcc
     8881 accattgtta cgtacagctg tcttgatatc tgttggaata gcgtccatat cagataataa
     8941 ttcttctatt gttttttcgc ctaattctgg gtatttctca attgctgcat ttaaattcgt
     9001 cacataagta ttgtgatgct tatcatgatg cagatgcatc gtttcttcgt caatataagg
     9061 ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg taagtcataa aaaaattcct
     9121 cctcaatgtt ttctttataa tgtctacatt acaaagagta acaaagagaa ggaaattctt
     9181 caattgttat gcctagtcgt ctttttcact ttgacctact tcttcttcaa aaacctcagt
     9241 ttctttaaaa ataaacaact tgctgaaaat atagttcgct aggataacaa taatttgagt
     9301 gatgattttc gccacatact cattgctgtt cataacattg atcaacaata ccattgcccc
     9361 catgtccaat ccgtaagaaa ggatacggta gaagaagaac ttaccgcatt caataagtgc
     9421 atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt ttgttcgtta caaaagcaaa
     9481 caatacagaa caaaaccacg aaatagaatt ggataaggcc atacttaaat caaaatattg
     9541 gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc aaaccaccaa aaaagagata
     9601 gactagtatc tcccaatatc cttttgcttc taaatatcct tttaactgtc gatactgctt
     9661 catgagccaa cctaccttat taaaaattct tatttatcat actctatatt tcaataactt
     9721 tcatctttat ttaaaaaaat taattttttt tgatcctttg cctttgtctc tgctt
//
LOCUS       DAXWLG010000096         9699 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXWLG010000096 DAXWLG010000000
VERSION     DAXWLG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.097"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            471..1673
                     /locus_tag="ACGE4T_001879"
     CDS             471..1673
                     /locus_tag="ACGE4T_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8387125.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(1850..3118)
                     /locus_tag="ACGE4T_001880"
     CDS             complement(1850..3118)
                     /locus_tag="ACGE4T_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFF8387126.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     regulatory      complement(3169..3236)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(3279..3926)
                     /locus_tag="ACGE4T_001881"
     CDS             complement(3279..3926)
                     /locus_tag="ACGE4T_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFF8387127.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(4191..4694)
                     /locus_tag="ACGE4T_001882"
     CDS             complement(4191..4694)
                     /locus_tag="ACGE4T_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8387128.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4818..5582)
                     /locus_tag="ACGE4T_001883"
     CDS             complement(4818..5582)
                     /locus_tag="ACGE4T_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFF8387129.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5690..5965
                     /locus_tag="ACGE4T_001884"
     CDS             5690..5965
                     /locus_tag="ACGE4T_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFF8387130.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(6313..7464)
                     /locus_tag="ACGE4T_001885"
     CDS             complement(6313..7464)
                     /locus_tag="ACGE4T_001885"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8387131.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(7484..8206)
                     /locus_tag="ACGE4T_001886"
     CDS             complement(7484..8206)
                     /locus_tag="ACGE4T_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8387132.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(8199..9650)
                     /locus_tag="ACGE4T_001887"
     CDS             complement(8199..9650)
                     /locus_tag="ACGE4T_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8387133.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
BASE COUNT         2834 a         2067 c         1673 g         3125 t
ORIGIN      
        1 gtgacagtga atgtatagaa aagcgagtgg ataaaaactg atttttcatt ctggcctact
       61 tgttccacgt tttactatgt atacttcttg tctatcttgt tttttccatc aaatcatccg
      121 gttcattcct tccacagatt ttgttgccgt taacatttta ttatgcaact ggtttattgc
      181 ttgaccgata ttttccaata aaaaaaaata tcgttttcgc attcattttc aatgttacag
      241 ttatttgtat ctacaaaagt cttcatcatg atcgaggtct aaaaaatctg ttaatttgaa
      301 gatttatgtt ttcgataaaa gcttttttca acataattcg ttataataga aacgtcgtga
      361 aaaaactgat tgactattta gtaactaact tttctatatt cgatatattt actactaaat
      421 gatgctatct tttttaagaa aaaatttaca aatcgaaagg agttcattca atgaatctta
      481 ctagagacct gatattttta gttatctata ttttaggaat gtcaggcatc ctctattggc
      541 gcaagcgcca aagcgatccc gctctattct tttattgtgg gacgtggact tttatgatga
      601 tggtcactta cttcgtcgtt ttagaagttt tttatcatac attttatttt ttgattccat
      661 tgctattttt cagtattttt gcatttttct attttaagga aaaacgtagg ttaatcaatg
      721 gcgtactttt taatacgttt ttgattgtct ttggtggtta tctagggtgg ctactaatag
      781 agacgcaaaa tatctttatc ggtgggttat tggctttgat cgggatacca atattaattc
      841 ttttggcaat cggtatctac attttgatca tttttttatt ttggaatggt gttgtagtca
      901 tgaaaaaaga atcccgttct ttggccaacc ttttgactct cttgttagga gtcgctttga
      961 ctatcttttt gatattggac tatacattag tccattggct cccacaatgg gcaaatgctc
     1021 tatttgctgt cattccgttt atttttgctt atctactcat cgttttcttt aattttatga
     1081 ctgtttcaat tttgtatcag ttcaaccgtc caaggtataa ccaagattat atcgttgttc
     1141 tcggcgctgg cttgattgat ggtgaacgcg tatctcccct acttgctaaa aggatcaata
     1201 aggcgatcgc attttattgg gcacaaagta aagcaacttt gaatccgcca gtcttgctca
     1261 tgtcaggagg aaaaggaacc gatgaaaaag tagctgaagc cattgcaatg aaacaatatg
     1321 ctttagaaaa agggattccc gagcgtgata ttttagtaga aacgaattcc acaacgacgc
     1381 tagaaaatat gctgttttca aaagaaataa tggatcagca aatgaatggg ccttatcgcg
     1441 cgatttttac ttcaaataat taccatatct ttagagctgg actatatgcg cgacaagcga
     1501 aactcaatgc aaacggaatc ggtgcaaaaa ctgcactata ctacttaccg aatgctatct
     1561 taagagaatt tattgccatc cttgtcttac acaaaaaacg ccatcttatc atctgtggct
     1621 taatgattgg ctttgcagta ttttctgctc ttattctcaa atttgctcaa taaaaaagag
     1681 cgtgggacaa aactaaaaat cagttttgcc acagactcta actccgaata aacggtggga
     1741 gcagaagcaa ctccttcgga aataagcgaa attcacaaaa atttaaaaag caattttcgt
     1801 gaatttcttc ttatttctcg gagttaaaca cttctgtccc aacctctttt tatttctctt
     1861 tttgtcgtga gattcttcca aggatcggta ggaacaagat tccttttttc acttcaactt
     1921 tttgcgcaga atagattcca ctatttcctg aacacataaa gctaatcaaa cagatcataa
     1981 agaagtagct ggctgcttga cctccaaaaa gttcgatccc cataacaaag caagcaatcg
     2041 gtgtattcgt tgctccagag aacaccccaa taaaccctaa tcctgctaaa aaaggtactg
     2101 atacatgaag aatgggtgct aatgtcgcac ctaaagttgc cccaatctca aacaaagggg
     2161 tcacttcgcc tccttggtat cccgcgccaa gtgatagaac ggtaaagcca agtttcccaa
     2221 gaaaatcata aggatgagca cttccattaa atgcatcttg taataaaggt aagcttaatc
     2281 cgagatatct ttgcgtctgt aaaatcacta ctacgagtac cacgactgct cctccaataa
     2341 agttgcgtaa aactggatgg gggatccgct ttgcatacca agcttttaac cagacgatcg
     2401 aacggctaaa gagccagcca atgataccaa atgcaatgcc cgcaagcaac aatttgaaga
     2461 aaagtgaagg gtcccatgca ggaatcgccc ccatttgata atgggtatgc ttgacattga
     2521 aactttccgt tacaaagttc gcaaaaagtg ctgcaaaaaa gcttggaaac agtgcttcgg
     2581 ttctcacttt tccgattgcc aatacttcta atccaaacaa tgtcccagcc aagggtgtcc
     2641 caaatactga gctaaaacct gcgctgatcc cactgatgac aagaatctca cgctcgattt
     2701 tgttcaattt tagccaacga gcaaaattct cagcaagtgc cccgcccatt tggaccgctg
     2761 taccctcccg accaacagaa ccaccaaaaa gatgcgtggt aatggtgcca aataaggtca
     2821 gtgggatcag ccgtaatgga atcttttcat ttcctccatt cccttgatcg atcactaaat
     2881 tgtttccccg ggaagaattg cccccatacg tcgtatatag ataagcaaaa agtcccccac
     2941 ttatcggcaa aagaaaaagt aaccaaggat gctccagccg aatagctgtc acatcagtca
     3001 aactagttag aaagaatgcg gataaacttc ccataaaaat acctaaaatt gctgtgatca
     3061 ataaccattt gatcatatag atgatcacct tcaatggatc aggtaatcga ttttccatct
     3121 catttcctcc tcaaaaaaac gcctactaaa aaaaggggta gccccgtttc tttagtaggc
     3181 gtcattagtt atcttacgat caacgtttaa aggcgaacac catcacctat tcacttcacc
     3241 aaataagata gcatattttt cagaaaaata caatgaaatc accgctcacg tttcactcgt
     3301 tctgtgaatt cgtaaaaagg agctaattgt accaactgct gattacattg gtcactatca
     3361 tgacaaatat aatttccttt tttcgtgtat gttccatctg aaccagtttt tgtggtagaa
     3421 agaaagagtg aaacatttga agttctttga caaatcgaac aaattccttt gttgattgta
     3481 ggagataatg ttccactgat cccttttagt ttttcttctt cataatagat cataaatttt
     3541 ttttggctac ctggatcatt ccaaccaata taagtatgtt cccttaaatc cagttgattc
     3601 caatctggtt gttttaactt tttcactttt cggaataatt tatcgatctg cggttttgtc
     3661 ggttgtatga atggaagaac gatcactttc aattcctcta atagtcgttc tgctttttct
     3721 ttttttagag tgacgtctag taccttttcc ataaattcat ttacagctgt attaggatga
     3781 ccaagtacct cgtggatctt ttccgcagct aatgcttgga ctgtttggac tgtctttgga
     3841 tcattcactg aactataggc gcttaacagc tgctcgactt ctcgcttgat aaaataatat
     3901 tgatatggtt tgattgtttt ttccatgact ttgagtcctt tcttctttca aaatcggcta
     3961 ctttgaagaa aaaagtgatc tttctatcaa agtgccttta gataaagccc tcaaactctt
     4021 ttaaataaga tacacatcgc atcttatcat cacccccttt atttttatgt tggttatgta
     4081 ctttttcatc tcaacaaagt aaacactaaa aagaccaatt agtcaatgtc taaacaaaaa
     4141 acccgaggat ttttttcaaa atactcgggt caaatactta gttaactacc ttaaatcgat
     4201 tgttctttac cgaaatatcg ttgccatttt tgtttcattg aagctgtcat tggttgagct
     4261 gcttctttga ctgcacaata tttatccaca aaagtaacga tataaccttc tttatattta
     4321 ggaggggcaa tcgttgctcc ccacatatgt ttcaaaataa tatcacgctc tagatctgaa
     4381 agctccgtga ttttttcagc atttttcaca gcgatccttg ggtgaacata cgcatgtgta
     4441 ccttcatcga acttcgtcgt acgccaatcg tagtaaaata aatcatgtaa aaggcctgct
     4501 cgagcagtcg ctcttgcatc accgccccat tttttcgcta atttgtagct atagtaagac
     4561 acactgattg aatgctctaa tcgtgtcgag tggacatgtt gcgtgtaatt tgctaatttc
     4621 tttactgcat cagtttctaa tagatcattt atataagaca tatattcttc gtcttcacgc
     4681 catttttctg tcataattat cgatctgcca cctttttgaa ttccatttat ctttgaaatt
     4741 gaacttagta taccactagt gttcgtaata ttctagtgag gttttcgcaa aatgcagaaa
     4801 gcttaatagt tccttaacta tacggagaag tgataaagca tgatgcccgc tgcaactcct
     4861 acatttaaag actccgcatt tccttgaatc ggtatataca catttttatc cgttagcgcc
     4921 agcacttctg gacgtacgcc ttgaccttca ttccccatca cgatcgcata attttctgtt
     4981 gtctggatat cattcaggga cactgcttct tcattcagtt ctgttccata ggttataaat
     5041 ccttgctctt taaggatagg caatagtttt gttagatcct cttggatgat cggtaaatga
     5101 tagttactcc cctgcatgct tcttaatact ttggtactgt agacatccgc cgttccctta
     5161 cctagtacaa cacaagatag accaaaagca tcggctgtgc ggatcattgt ccctacattg
     5221 ccgggatctt gtacgttgtc gagcaacaac caaccacctt caaaattcgg tatctctctt
     5281 ttttcagctt ccaccactgc catgatccct tgcggagttg gtagatctga aacagttgcc
     5341 atcacttctt ctgatactag tgtaacagaa agcgcctgat tttcttcaag ccaactagcc
     5401 cactcagtca gaccgcgttg cgtaataaaa agttcgttga ccttcgcatt tgaggcaaca
     5461 gcctcctcga tcaggtgaaa cccttcaagc agatattccc ctttttcttc acgatatttt
     5521 ttcttatgta atttttttaa ttctttgatt cttggatttt ttgctgattg gatctctttc
     5581 acatttttca cctcaactga ttataccatg gattagatca ttgcacttgt tcagtagcaa
     5641 aaaagtcggt atcattaatt taaaaataaa agaaagaacg gtgaataaaa tgaacaaaat
     5701 caagatgaac gtgcaaggac gtgtacaaag cgttggtttt cgctatatga caaaaatcgt
     5761 agcggatcaa ttagggatca ccggaacagt gaaaaatgaa gatgacggct ctgtcacgat
     5821 cgaagctgcg ggcgacgatg aggcaatcaa aaaattcgtg gaagaagtca aacgatcacc
     5881 aagccccagc ggacgtgtcc aatatgtaga ccttcaggaa aatccgatga tggaggacag
     5941 aaaaaagttt gttgtaatag gctgaccttc tagaaatgaa taggaaatgt cagaaaaatc
     6001 ggactgtctg tatgtagatc gaaaaagttt gcccctctct ccatcctaga tcgacaaaaa
     6061 aacattctta ttagcgagtt tagtaaccag tgataggcat acgtaaaagc gggaggactt
     6121 caaaaatttt ggttcaataa aatggagtct ataatatttt atggtagtgg aaaatcgact
     6181 ttgattttcc actaccactt tttcattgtc aaatcctgac gctgcccagt caacaagcca
     6241 actctcgaaa ggatcatttt ccctctttgt tctaatggat acccatagct gttttcaaat
     6301 atacagtttt atctaaaaat ttagttttgc taaacgatca aaggcttcac ctaagatttc
     6361 caatggctga gtcgctgcta gtcgaatata gttgtccccg atcgtttccc caaacgcttt
     6421 tccagggaca aaaagtacgc cgcttttttc taaaacctct tccacaaacg ttactgaatc
     6481 gagcccactc ttactgatat taataaaagc atacatgctt ccagaaacag aagataaaga
     6541 taagtaaggg atttctgcca ctcttttctc aatataagct aatcgttctt tgaatacttc
     6601 aacaactggc ggtacaagct gttcatagtg ctttaaggcg tagattgcac ctctttgaga
     6661 agcagtcggt gcggaataag tgatgttttc attgatcaac ccagcaattt gattgatgta
     6721 tgctggtgcg atcatgtacc ctaagcgcca gccagtcatc gcaaacccct tcgatacacc
     6781 actgacagtg atcgtattat cgggcgcaaa tcgcgccatc ggtacgaact tctctttaaa
     6841 ggcaaagtct tcataaatct catcggaaat gatatagagt tcattttcga ttgcgacttc
     6901 agcgattttt ttcaacgtgc tttcagaaaa aacagctccc gtaggattat tcggcgaatt
     6961 gatgacgatc acctttgtac gatccgtaat cgctgctttt aaatcttcca catccaactg
     7021 gaagcccttt tcttcatagg taggaacaaa aacaggcttg cctccagata aaatgacttg
     7081 ttcccgatat ggtgaaaaat aaggttcatg gatgatgacc tcatcctctg gatctagtaa
     7141 tgcctgtaaa actagataca tcccgtgcat tgctccgact gtagctcgaa cttggttgca
     7201 ttcaacgctc aatccatatt tcttttgata aaaatctacg accgttgaca agaattcttt
     7261 gctgccatca ggcgcagtat aattagtagc accatttttt acatctgtat aagctgcttc
     7321 aataatcgtc ggatcagtga tcaagtcagg atcaccaatc gataaatcga taatatcctc
     7381 tttttccttt gccaaaatag caatattcat caacagattt tctgctggtt gctgaaaccg
     7441 gcgtgcaatc ttacttttgt ccatcttact tcctccatct ttgttacaac actttgttca
     7501 agaaatcctg tgttcgacta ttttgcggat tttcaaaaat ctctttcggt gttccctctt
     7561 cttgaatgat gccgtcagcc ataaaaatca cacgatcggc aacttcttta gcaaaaccca
     7621 tttcatgagt gaccacgacc atcgtcatcc cttttttagc taaatcctgc atgactgcca
     7681 acacttcacc gaccatttca ggatcaagtg cagatgtagg ttcatcgaaa agcatcacat
     7741 caggattcat tgctaatgct cgagcaattg ctacccgttg ttgctgtccc ccagataaac
     7801 tggtaggata actgtccact ttttccttta gaccaacttg gttcaataat gatagagcat
     7861 gttcttttgc ttttttaggg tcttcttttt tgatttttac aggtgtgatg gtcaaatttt
     7921 cgagtacatt catatgggga aacaaattaa aactttgaaa taccattccc attttttgac
     7981 gaatttgatt gatatcgctg tcaggggctg taatgttttg tcccttaaaa aagatttcac
     8041 cagaagttgg ttcttccagc agattcatac aacgtagaat tgtacttttc ccacttcctg
     8101 aagggccaat gatcaccact acttctcctt ctttcacaga aagatcaatt gattttaaga
     8161 cttcattctc gccaaaactt tttgacagtt tttttacatt aagcattcag acccattctc
     8221 ctttcaacta aagctaatat acgggaaata ctgaaggtca acacgaaata aatcaaagag
     8281 accaacacat atggtaggaa tggtttgaag ctagcacctt gtaccacgcc cgtttgaaag
     8341 atcaattccg ataccccaat gaccgaaaca actgaggact ctttgatgat cgtgacaaac
     8401 tcatttccta gagctggaag aatatttttg attgcttgtg ggaagatgat gaaacgcatg
     8461 gcttctttct ggtccattcc taaagaacgt gcagcttcca tctgtccttt gttgaccgca
     8521 ttgatcccgg cacggacaat ttcggctaca taagcagcac tattcaacga gacagcaata
     8581 caacctgcta ccagtttcga tagatccaat cccagtactc ctgttccgaa gtaaacgata
     8641 aagatttgaa ctaagagagg tgttccccgt acgtattcga tataaataat ggctaatgcc
     8701 tttaaaaatt tattcttacc caatttcatc agtgcaagca aactaccaaa gaccattcca
     8761 aacaatacac ccacaaaagc taataaaata gtgtattttg ttccaatcaa ataatacgga
     8821 ccgtattttt gaagaaagga ctgatcatct tggaacatca attgattcgc ttgtttttga
     8881 taatctttca gcaagccgtc gtcattgatc gttttgattg acgcattgac atgatttttc
     8941 agttctggtg tattttttga catcgcaata gctgtggttt tttcgccttg cgcaaatttt
     9001 actggagtca aagctacttt cttattctgt tgggcatatg cttctgcaac tggttgttcc
     9061 agcacaacac cttcaacttt ttcattcagc aaattattga tcagatcagg aactttttgc
     9121 agggaggtca actctgcacc agtcagttca tttttcacta actcctcttg tgtggtttgc
     9181 ttttgcgccc caacggctaa gccagaaaag tcggctgtcg tcttaaattt atttttgtcc
     9241 tctttacgaa cgagtacttt ttgctgtacc acgaaatatg ggtcagaaaa gtcaacttct
     9301 tgtaaacgtt cttcagtcac cgccatacca gatataacta aatcgatctt gccagttttc
     9361 aacgcaccta ataatgcatc aaaaccaagt tcttctactt ttagtttcac acccatatcc
     9421 tttgcgatct tttcggcaat ggatacatca aaaccaacga ttttatcctt accatccaca
     9481 ttcgcatgga attcataggg cgcatagtct gcagataggc caacgactaa ttctccactt
     9541 tgttttatcc gctcgtaaat ttcatctttt tctgcacctt gtacttttac tggtaaaaaa
     9601 accagtagaa acatcagcgc cactaataat atgtttattc cttttttcat ccatttggcc
     9661 acctttgaat aataatgtaa tcagaaaagc tataaaaga
//
LOCUS       DAXWLG010000097         9679 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXWLG010000097 DAXWLG010000000
VERSION     DAXWLG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.098"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            32..3310
                     /locus_tag="ACGE4T_001888"
     CDS             32..3310
                     /locus_tag="ACGE4T_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFF8387134.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3851..5758)
                     /locus_tag="ACGE4T_001889"
     CDS             complement(3851..5758)
                     /locus_tag="ACGE4T_001889"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFF8387135.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5888..6763
                     /locus_tag="ACGE4T_001890"
     CDS             5888..6763
                     /locus_tag="ACGE4T_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFF8387136.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6843..7817
                     /locus_tag="ACGE4T_001891"
     CDS             6843..7817
                     /locus_tag="ACGE4T_001891"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8387137.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7997..9142
                     /locus_tag="ACGE4T_001892"
     CDS             7997..9142
                     /locus_tag="ACGE4T_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8387138.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9279..9623
                     /locus_tag="ACGE4T_001893"
     CDS             9279..9623
                     /locus_tag="ACGE4T_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFF8387139.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3151 a         1795 c         2050 g         2683 t
ORIGIN      
        1 cgctttctaa tttgttacgg gaggaataca cttgttaaaa aaacgtataa aatacagtgc
       61 gctaatgatg aatctcctta tgctcagtac cccggtttta atttctgtcg ttgaagttgc
      121 tgctaacgaa caacagtctg tgaacgaaga aaatgcacac gtgaacattg aagggatgtc
      181 aacaatggag gaaataccaa gaactagtga agatatggat acggaagaag gaaatgatcc
      241 tatgatgaaa gaatcagaaa atgaaaagac aaaaaagcaa tctgaagaag caaatgaaga
      301 gtggaaaggg gttgttttcg gagaaagcac aagtgctagt aggtatgcca ttgaaccatt
      361 agaagacggc gtgcggctct cttccacgaa taacggcgga aaattccagt caagcggatc
      421 agatggaatg acctattatt atcaagcaat ccccaaagac atcaacttta ggatgcgggc
      481 gacgatcgaa atagaatctt ggacctatac gaatgcccaa gaaggatttg ctttaatggt
      541 aagagacgca gtaccaaaaa atcatttgtt tgggcaggca ttttattcaa attcatttgc
      601 catactaggc agtcggatcg aatatgtctg ggactcggat agacaagaag tggtcaacac
      661 gagcggcagc aaatatacga tgcgacttgg attggggaca cgggcaatca caggaatatc
      721 atctgaagat ccgcaagctg cgccagctcc tgggagtgtc tcagtggaaa caacaccttt
      781 agagacttca gcagggttct tggaaaaaga aacaggttct tataatatat tcggaaacgg
      841 tcatgggaat ctgtttccgg ccacgaactc gattactaaa ctggacgtag agatgaaaaa
      901 aacgaatact ggtttagagg catattatta cgatcctgaa acaggagaag aaatggcttc
      961 tgacatcatg tatgactggg aaaaattata cgcttcagat gaatcggtta tttatgcagg
     1021 atttgctgca gcaaggaata tgacgatcaa ggtagaagga atcgaagttg cttactcaga
     1081 tcctgcgaca gatcctcctg ggcaagaaag acctactcgc ttgattgatc cgatttacac
     1141 tgtgacctct agtaatcatt caggaaacca agatcatact atgtccttta gagcaaatgc
     1201 tgacggatta ttgacaatta aaaataaagc aacaggagag gtgcttaaga acgcaaaaaa
     1261 cttggctatc actgcgaatc gggaatttga ttatcagaca aaactgatgg aaggaacgaa
     1321 cgaatttact ttcagtttta cgccagataa aaactatccg ccagaagaat acgtagagat
     1381 gactagttat gaaacaaaag agattcttca tacagtggac taccgtaaac ctaaatattc
     1441 gacagtttat gtaacaccag aagggtcaga tgcaggaaac ggaagcagac aaaatccatt
     1501 atctctgaca caagcattga gatacgcata tgctgggcag acaatcgtca tggcgcctgg
     1561 aacttattcc ttcgaaagag gattgacgat tccaaaaggc gttaacggga caagagaaaa
     1621 cccgattcag ttgattgcag agaaaaatcc agaaaacaag cgtcctgtac ttgattttaa
     1681 aggaactgga aatgggatga cactgttcag tcactactgg ggtttacgag ggtttgacat
     1741 tacaaatagt gcagctatgc aaaaaggatt acagatttct ggaaatcata atttgatcga
     1801 agagattcat gcttatcgga atggaaatac aggaatccaa atcagcggta gctcaaatga
     1861 tcctttcgaa gcatggcccg cgcataactt agtcaagaac tgtacatctt ttgaaaatgc
     1921 cgatccagga tttgaagatg ccgatggatt tgcggccaaa ctgacaatag gagaaggaaa
     1981 tgtatttgac ggttgtatcg cgtatcataa tgcagatgac ggttgggacc tttttgctaa
     2041 aaatgaaaca ggttcgatcg gaaaagtaat catcaaaaac tcagttgctt atcgaaacgg
     2101 ctgggtacca ggaattgagg gagaaggtaa cggtaatgga ttcaaaatgg gcggttcttc
     2161 tttaacgggc cctcatgaat tgatcaatag tttgtctttc gaaaatcttg cgaaaggaat
     2221 cgattccaat agcggaacag atattatcgt caatagcagt acaagtttta ataacggttc
     2281 atataatgtg gctctttata cctcttcagc aggaaacaca gactaccatg cttctggtat
     2341 cttatctttc cgaacagaga atcttgagat gagagagcag atccgcccat tgaatcaaaa
     2401 cgaagatcaa atctaccact cgttgaacta ctattggaat gaacgagaga aacaatcaga
     2461 aaatactctt ggacatacca tatcaaaaga ttggatcgag tctctaaata catgttcgaa
     2521 agaaggtcag cagcctgtta cgcgctatga aaatggaagt atccatgttc atggactgat
     2581 gcagattaaa ccggggaatc gtcaaacaga aaaggaaaga gtggatggtc ttccattatc
     2641 tgtaggtgct atttttgacg gagaaacctc accgtcttcg gaatatccag agtatgcaat
     2701 cgtacaagaa ccaaatcaga caaaaccaaa tgaacaaaat aatccgatta ttatacaaga
     2761 agcaaatggt gtggcggaga atgcagaagg aaatatcatt gaactgaagc cttttacagt
     2821 ttttgtgtca actttcacac caaatcaagg aacgcctgtc tttgaagata tgcgttattt
     2881 cccagaaaag atccaaatcg ttgaaacaca gtcaagtgct caattttcta atcataccgc
     2941 tattttttca gcaccttcaa aaggagaata tacgctagaa gtcacttatc gttcggaaat
     3001 atttgatggg gaaaaatggt ctgctcatac agaatctgtt acccagcaaa aagcgatcac
     3061 tgtgcgagaa actgccgatg tgcccccggg aaatgacaaa ccagaaagag aaaatccagg
     3121 aaatgaatca ccaaatggaa aaccaaatgg aaaaccttct catccggata ggacagcaaa
     3181 agaaagtgaa aaaaatggca ctcaaggact cccaaaaacg ggagaattta ataacccgtt
     3241 gctagcatta gctggaggaa ttttgttaat cggtgtagtt gtttatgtca tgaaacagcg
     3301 aaaaaaataa gtggaatttc tccttttatg atgtaaaaca attgactgag gaaagtaaaa
     3361 atagaaggaa atgagaccaa tagatgtctc acttccttct atctttttat agatgaggtt
     3421 ctccttgctg cttttgattg gctgttctta ttacttgatg caggacagct ttttcacaat
     3481 ctctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaca aacggcaaaa
     3541 tatgaggaac tatttttccg tttttttctc taatactcag gtcaggacgc tgatttcaca
     3601 acctctcgat aaacggtgtt caaaagccag tttctcggta gtaagtcaaa tttatgcaaa
     3661 aatgtggaaa gccattttca caaaattttt cttactactt gaaactaagc agctttttca
     3721 caacctctaa tacatggtat tccagaagta acttcttcga aaaataagcc gaaatgtcac
     3781 aaaaatttga gaagcgattt tcccaaattc cttcttattt cttgaagctg accacttctg
     3841 gcacaacctc ttaccagatt cgcactcttt tttctggcgc tagatacata gcgtcttgtt
     3901 ccgtgatacc aaatgtttca tagaattcgt caaggttttt tggttgcacg tttgctcgga
     3961 gtttagcagg ggcatggaca tcgatttgca gtaaaagttg ttggtattct ttttttgctt
     4021 ttgtacgcca aatcgttgcc caattgacaa agaattcctc gatagaaaga tctgattccg
     4081 tttttgctgc ttccaatgca cagcttagac cgcctgcatc agcaatattt tccgaaacgg
     4141 ttaattttcc gtttactttt ccgtcagcaa aaggcagacc atcgaattct tcgatcattg
     4201 cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt ccaccagttg ttcaagttgc
     4261 catattcgtc aaataaagcg ccgttattat caaatgcatg ggaaatctca tgagcgataa
     4321 cagctccgat tccaccataa ttagcagagc ttgattgttc tagactgtaa aatggtgctt
     4381 gaaggatcgc tgccggaaag acgatcacat tccgaaatgg atgataataa gcattgaccg
     4441 tgtcagcact catttcccat tctgaacgat ccactggttt gttccatttg ctgaaacgat
     4501 cttttaatgt gagtttgctg aaaacaagag tatttgaaag caaagtacca ccctgatctg
     4561 caggtactgt tttgaactgg tcgaataatg ctgggatctt atcggggtag cctacttgga
     4621 tacctaagtt atttaatttt atgacggctt tttcttttgt tgcatcccca agccagtcat
     4681 tttcttctag acgtttttta taaacggcga tcattttttt caccatttgt tctacgtctt
     4741 ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc gccaactact tgatcgaatt
     4801 gtccagaggc aaggtaatac gctgcttttt tctgaggcat tgcttcgtct gttccagaca
     4861 atacgcgaga atagattccg ctgacttggc ggaattcttc tgatagataa ccactaagag
     4921 aacgtactgt tttaatgatc atccagcttt tgaataacgg aaaatgagct tctgtcagga
     4981 tgtcattcaa atgatcgaag tacactggat cagttacgat cactttatct ggagttgtcc
     5041 caatcaaacc cgtgacaagt tttttcaagt ccagtttatc tgtgtattta ataaatgtat
     5101 caaaagattg cggattatac attttgctgt aatctgcgtt ttcttctgca ctttttacat
     5161 gaggagcaat caatttatca aattgcactg cttgttccgt gattgcttct gcctgacttt
     5221 tttctttgcc ggcaagctct aataactgaa cagccatatc aaagaaaacg cctattagtt
     5281 gtgcaccgtt gggatggtct gatgcataat acgtcttatc tggaaggaat aaagaaggcg
     5341 gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc tgcatctaca tccagattaa
     5401 atggcaatgg taatgtatcc aacagccatt ctggaaattg cttattcaat tcgtcgaaag
     5461 actgcaatga ttcaactttt tcgattatag gttgaagcgg ttgagcatgc aactcatttc
     5521 ttcggtcata atcgtttgcc agttgataat atgcaaggaa atgtttcatt ttttctgaag
     5581 acactttttc tggatgggca agcagatcgt ctgtttctcc catcaaaagt tggtcgatcc
     5641 cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc tgcagggatt tttgctgttt
     5701 tttgccattc ttcattgact gcttcataga aatcttgttt caatcgttct ttgttcattc
     5761 atcgatcgct ccttttttgt ttcgtgtgcc cattatctta caaaatgaag gaattaacaa
     5821 atagaacata aaaaattgaa aattcgaaca atcaaactga tttgtttttt tcaatccttc
     5881 acttataatg aaaaaaagga ggcgatccca actgaatcaa gtagtggata aacctttata
     5941 tttcaaagta gataaaaaaa ttaaaaaatt agccagcact cagcagctgc agtcaagaaa
     6001 aagtaaattg ctttttttag ctctggtttt tgaagatcaa agttatgtga ttatcgatca
     6061 atcaggacat ccggtagaat attctccagc aaaatatact tatcaagagg gtctttcttg
     6121 taaaaaatgg aagctgatca atgaagagcc aatcgagtta tctagatgga tcaacagaaa
     6181 agaagatatt cccgttttga tcgaagaaaa aaggtcagga aaggaactcg caaattgctg
     6241 ggtgggattg ccagaagaac gttttttgag gtataagaaa tgggctaccc cctcaggcta
     6301 tctttgtggt acttacgcgg cagcagttct ccttgcctat tatcaagact atcgtaaggg
     6361 gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct gattcgttgg ttttgacgaa
     6421 ggcattgcgt agccagatcc aaccactagg acttcctacg attccttttc aggttagtac
     6481 agggatttct agttttttaa agaagaatgg caatcatgag cgtgcacgag caactcttct
     6541 aggttcatgg cagcgagcta caaaacgtat acgtgaaggc aaacctgtga tgattggtat
     6601 tttgaaagta cttggcagta catatggcaa tcattgggtg acagcgtacg cttattttga
     6661 aactgaaaca ggagaacgtt attacaaagt acacgacaac tggggcgatt atcataaagt
     6721 cattcctgcc agttggagta acggaacagt ttcacttcct taagaatttg ctgaaattaa
     6781 cagaaaatct tggaaaaata tgttaaacta tcgaagaata gagaagtttt ggggaggact
     6841 acatgagtaa taagtatcag gatgacacgt tgaagatttt tagtttgaat ggcaaccgtc
     6901 cattagctga aaaaatcgcg aaagtgttcg gaacagaatt aggtaaaagc gtcgtgaagc
     6961 aatttagtga tggcgagatt tccatcaata tcgaagaaag tattcgaggg gatcatgtgt
     7021 atatcgttca atcaacgaat gcacctgtca atgattacta tatggaacta ctgatcatga
     7081 tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt cgtattgcct tactatgggt
     7141 atgctaggca agaccgcacg gcgaaacctc atgaaccgat cactgcgaaa ttgatcgcta
     7201 acttgatcga agaagcaggg gcaactcgag tactgacttt agatctgcac acggttcaag
     7261 tacaaggatt ctttgatatt cctgtagata atttatttac gatgccgttg tttgctcatt
     7321 attaccgtca attaggtctg acgggagatg acatcgtagt tgtttcgcca aaaaacagcg
     7381 gtgttcagcg tgcacgtagt ctttcagaat atttgaacag cacattagcc atcgtagatc
     7441 atgcagatga tgcttcaact gagggtggtt atgtgatcgg tgatgttcaa ggaaagacat
     7501 gtatcatggt agatgatatc ttgaacacag gagcaacgct tgcacgagca gcaaatgtat
     7561 taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc acatggatta ttgtccgatc
     7621 atgcgaaaag tattttagac aatgcaccaa tcaaagatat ttgtatcact gattctgttt
     7681 tcacagaaga aaatcgtcat ccagaaaact taacaatcgt tacatgttca gaattaatgg
     7741 gtgaagcggt caaacgcatc catgaaaata caccgatgag tccgttattc cgtctggaag
     7801 aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt tgtgacagaa gtgagcagct
     7861 tcaaggagct gcgacaagac ttttgtcaca gctccttttt tctatgccca tgaaacgtcc
     7921 tctagacaaa aatgagctct tctttggttt gttcgctatt ttatgctaga atgtgcctag
     7981 caataatttg gaagggatga atgaagtgaa aactatttat ctggatcatg cggcaactac
     8041 ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc atgcaagaca cgtttggaaa
     8101 tccttcaagc atacatggat ttggtcgcca cgcgcatgaa aaattggaaa cagcaagaca
     8161 aatcattgca gacagtctgc aagcaaaacc tcacgagatc atctttaaca gtgggggaac
     8221 agaaggggat aatacagcta tcttagaaac agcgttctcg cgtcaaaaag aaggcagaca
     8281 tctaatcact acagctatcg aacatcctgc tgttcttaat accatgaaat atttagagac
     8341 aaaggggttt gacgttacat atctgcctgt agatgaaaaa gggaatctgt cgatcgaaga
     8401 ggtcaaaaaa gcgttacgag aagatacgat cttggtctct gtgatgtacg ggaacaatga
     8461 aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt ttagcggatc atccagctta
     8521 tttccataca gatgcggtac aagcgtacgg aaaccaaacg atccatccag aagaattagg
     8581 aatcgattta ttaagtatct ctgctcataa aatcaatggg ccaaaaggcg tcggtttctt
     8641 atataaacga gacggagtaa atatccctgc attgctttta ggcggggagc aggaagaaaa
     8701 gcggcgtgca ggtacagaaa atttagcagg gatctggggt atggcaaaag cagttgaact
     8761 gctgtcacct gaagaacgtg aagcacgtaa caacaaatat aaaggattcc agcaattgat
     8821 tcttgataca ttagaagcaa acgaaatccc ttatcaagta aatggtgatt taacgaataa
     8881 actccctcat gtgttgaatc ttcgcttaat tggcgaagca aatgatttgc tactgatgaa
     8941 gcttgatctt aatggggtcg ctatttctac tggttcagct tgtacagcag gaaatatcga
     9001 gccttcacac gtattagaag caatgtatgg agaagataca ccgatactaa aagaatccat
     9061 ccgtatcagc tttggtttgg gaaataccga agacgacatc cagtatttcc ttgatcaatt
     9121 agttcgtgct gtcaaaaaat aactagtgaa gtcattctta tatgaacaga tgtctgagag
     9181 aacgccttca ttttttaggg aacttaactg aaaaacctag taaaaatcag gataaacttt
     9241 tataataaag agaaactaat tttatcgagg tgaaaatcat ggcatttgaa acaacagcaa
     9301 cggtactggg cgcaccaatt agctatcgtt tgcatcctga tgcaaaaaga tatacattgc
     9361 gcgataatgg atttacagaa acaaacggag gcaattatca attgatccga ccattagatg
     9421 ccacgccgca aagcaaagaa ggattcaaat taaaaatcac tgtatcaaaa gatattcaaa
     9481 cattgaaaat gtcgatcact acagcaaatg gattgaaagc agtcaatatt ttcaaagatc
     9541 caaaacagaa aatgagccaa gataaatttt attttctgat ggatgggatg attagccgtg
     9601 ggttattcga aaaagtcgaa tgatctgtgg atataaaaag acaactagtt ttgaaacggc
     9661 ctattgttag atgactaac
//
LOCUS       DAXWLG010000098         9649 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXWLG010000098 DAXWLG010000000
VERSION     DAXWLG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.099"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(30..350)
                     /locus_tag="ACGE4T_001894"
     CDS             complement(30..350)
                     /locus_tag="ACGE4T_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8387140.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(498..2732)
                     /locus_tag="ACGE4T_001895"
     CDS             complement(498..2732)
                     /locus_tag="ACGE4T_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8387141.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3211..3477
                     /locus_tag="ACGE4T_001896"
     CDS             3211..3477
                     /locus_tag="ACGE4T_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFF8387142.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3477..5204
                     /gene="ptsP"
                     /locus_tag="ACGE4T_001897"
     CDS             3477..5204
                     /gene="ptsP"
                     /locus_tag="ACGE4T_001897"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFF8387143.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5368..6414
                     /locus_tag="ACGE4T_001898"
     CDS             5368..6414
                     /locus_tag="ACGE4T_001898"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8387144.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6431..7654
                     /locus_tag="ACGE4T_001899"
     CDS             6431..7654
                     /locus_tag="ACGE4T_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8387145.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7743..8630
                     /locus_tag="ACGE4T_001900"
     CDS             7743..8630
                     /locus_tag="ACGE4T_001900"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8387146.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8716..8946
                     /locus_tag="ACGE4T_001901"
     CDS             8716..8946
                     /locus_tag="ACGE4T_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFF8387147.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9003..>9649)
                     /locus_tag="ACGE4T_001902"
     CDS             complement(9003..>9649)
                     /locus_tag="ACGE4T_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HFF8387148.1"
                     /translation="FVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLA
                     LRAQLPDLQEMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDI
                     ELVTYFIRRIQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPD
                     FYYEKKEAHLSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
BASE COUNT         3073 a         1711 c         1968 g         2897 t
ORIGIN      
        1 gctctttaat tattatgttc aaaatttgtt tacacggcta atgtagcgcc taattcatgt
       61 tttggaatcg agatttcaac aaggcctgcc aattcgatta ctttgatttc atcctcgtgg
      121 taaacggcgt ctttgatttt atctaagaaa taatttttag catcttcgat ttctttcggc
      181 tggattttac gtttgtcatt gatcagcaga atttcattat gttcaggggc ttcagtgtag
      241 acaatagtgg tatttccggc tgtgtagacc ttgactagat gggcatcagt gttttcaagc
      301 tggtttaata caagacggga atggctgttt gttacattta ccaatttcat ggcgctcacc
      361 tcttttatgt atttttaatt gcttgtttct cttattataa tttctttttt ataaaatgaa
      421 cagtattttg acttttctga aaaaagaatg agacggcatt tctcaaagaa ataccgtctc
      481 acgctataaa actggattta ttcagccgtt tcttcggctg attctttagc caaacgttct
      541 ggtttagaag taacgatgat ttttccatct ttatccagtt ttgctttcaa ctcatggata
      601 gaagggtgat caagataaaa ttcagcgata ccatcttcga tttgttcttg gatcgtgcgt
      661 cgaagaggac gagcacccat agatggatca tagcctaagt caactaattt ttctttgaca
      721 ttcgtaggaa cttcgatgtg aagttgctgt gctgccagca tttggttgac atcgtctagc
      781 attagtgtga cgattgtcat caagttttct tttgataatg cactgaattc gatgatcccg
      841 tcaaaacggt tcagaaattc tggtgtaaag tagttgttta attggttcaa tacagaacga
      901 gtaacacctt cgcgtgccgc accaaaacca acgtttgctt ctactttacc agttccggca
      961 ttacttgtca taatgatgat cgtatcttta aagctgaccg ttcttccttg tgcatctgtc
     1021 aatcgaccat catctaagat ttgcaagaac atatgcaaaa catctggatg agctttttct
     1081 acttcatcta acaagacaag actgtaagga tttctccgta ctttttctgt taattgacca
     1141 gcttcttcgt atccgacata acctggagga gaaccaatca atttagagac actatgtttt
     1201 tccatatatt cagacatatc gaaacgaatc attgaatctt gtgaaccgaa tagttcgtag
     1261 gcaagttgtt tcgcaagctc tgttttacct acaccagttg gtccaacaaa taagaaggag
     1321 ccgattggac gattctgttt gtttaatcca acacggtttc ggcgaatcgc tttcgctact
     1381 cgatctacag cgttatcttg tccgatgaca tgagctttca agtcatctgc taagtttttc
     1441 agttgtgtct gttctttttc ttttaattca cccactggaa tacctgtccg ttgttcaacg
     1501 attttttcca tatctttttc ggtaatcaca ggtgtttcct cttcagtgag ctgacgttct
     1561 ttcatttttt gaagtttatt gatctgatcg cgataataag ctgctttttc gaaatcttct
     1621 tctcgagaag ctaaaacttt ttgttcttct gcttcttgca gttttttctc gattgtttta
     1681 ggatcaacga tttggatcgt caagtttttc ttagaacctg tttcatctag caaatcgatg
     1741 gctttatccg gcaagaagcg atcttggacg taacgattgg ataaagttgc ggctgcttcg
     1801 atggcctcat cagtatattt tacatgatga tagtcttcat aacgtttttg caaacctttc
     1861 aaaatactga tcgtttcttc gacagttggt tcatctaccc gtacaggttg catacgacgt
     1921 tctaaagcag cgtctttttc gatgatgcgg tactcattta aggtagtagc accaaccatt
     1981 tgcaattctc ctcgagctaa agctggtttt aaaatattac cagcatccat atttccatca
     2041 ccagcagaac cagcaccgac gatttcgtga acttcatcta tgaataggat cacattttct
     2101 gcttgacgga tctcttcaat caatttttgc atacgttctt caaattgtcc tcgtatacca
     2161 gtaccttgga ctaaagatac gacatctaaa cgaatgactt ctttgtccat taatttttgc
     2221 ggtacatcac catctacgat tttttgtgct aaaccttcaa caactgctgt tttaccaaca
     2281 cctggttctc caatcaagac aggattgttc ttggttcggc gatttaaaat ttcaatgact
     2341 cgtttgattt cgtcatcgcg tccgatcaca ggatcgatat caccttgtcg tgcttgttct
     2401 gtgatattga ttccgtattc tcccaataaa ccttctgtat tgccttgagg tggctgacca
     2461 ccattgaagt tgttgtttcc gccaaattgt gttggtggtg tttgctcata atttccttgc
     2521 tgttgcattt ggcgagacat ggaacggaaa agatcgtcca aactgccaaa tccaaatgga
     2581 tcattttggg ccatattagt cagacctcca ttttgttgat ttttgatttt ttgataacag
     2641 ctctgacagt aatcgagttg tgtccgctga ccattgacag tggcatacaa gtgaatcgtt
     2701 gcctcattct tgccgcagtt ttgacaaagc atgaatcatt cacgtccttt cacaaaaatg
     2761 ggtacatcca tattttacat ctcgtttttc atgaggtaaa agaataaccc ctagtttttt
     2821 aagagattta tgaggtagaa aaggtgaaat ctctattgac cattcctgac ctttgactct
     2881 tattatacta aaaataaaaa gggaagcaag taattcgttt catatgaaaa caaagagggt
     2941 caaaggaaag aatgataaac gtcaggagaa gagaaagcga caagaaaaag aaaataaaca
     3001 cgtttgttat aaaatgtgaa gaaaaggatg aaaaagaatc cggaataatt tgaatacttt
     3061 tatctgaaat tgaatgaaaa ctgttgtatc taacgaaaaa aaatgttaat ctttacagat
     3121 gaaagggtgg catttgttag gattctctga attactaaaa aagaaaaccc tcacataaaa
     3181 acaacactca cttaaaggag accgattaat atggaaaaga aagaatttca cgtagtagca
     3241 gaaactggga tccacgctcg tccagctaca ctattagtac aaacagcaag caaatttaac
     3301 tctgatgtaa acttagagta taaaggtaaa tcagtaaact tgaaatcaat catgggcgtt
     3361 atgtctttag gtgtaggtca aggttctgat gttactatta ctgcagaagg tgctgacgaa
     3421 gctgatgcaa tggcagcaat cgttgaaaca atgaagaaag aaggcttatc tgaataatgg
     3481 ttgaaatgct aaaagggatt gccgctagtg acggtgtagc cgttgcaaaa gcttacctgc
     3541 tagttcaacc ggatttaaca ttcagtaagg caacagtaga agatactgcg gctgaagaag
     3601 ctcgtttgga tgctgcatta gctaaatcta ctgaagaatt gcagcaaatt cgtgaaaaag
     3661 cagcgcaaag cttaggtgaa gcagaagcgc aagtatttga tgcccatttg atggttcttt
     3721 cagatccaga aatggtaggt caaatcaagc aaaatattaa agacaacagc gtaaatgctg
     3781 aatcagcgct aaaagaagta acggatatgt atatcggtat gttcgaagca atggaagata
     3841 atgcttatat gcaagagcgt gctgcagata ttcgtgatgt tgctaaacgt attttagcgc
     3901 acttattagg cgttacttta ccaaatccat caatgatcaa cgaagaagtg gtcgttgttg
     3961 cacatgactt gactccaagt gatacagcac aattagatcg caattttgta aaagcgtttg
     4021 taacagacat tggtggacgt acttcacatt ctgctatcat ggcacgttct ttagaaattc
     4081 cagcaatcgt tggtacaaaa gaaattactg ctaaagtcaa agaaggcgat attttggctg
     4141 tcaacgggat cgaaggcgat gtaatcatcg atcctacaga tgaacaaaaa gctgaatttg
     4201 aaaaagcagg tgcagattac gcagcacaaa aagcagaatg ggaaaaatta aaaaatgcag
     4261 aaactgtcac agctgacggc aagcattttg aattagctgc aaacatcgga acaccaaaag
     4321 atctagttgg tgtccataat aatggtggag aagcagttgg tctgtaccgt actgaattcc
     4381 tgtatatgga ttctccagac ttcccaacag aagatgacca atatgaagct tacaaagctg
     4441 tattagaagg tatggaagga aaacctgtag ttgttcgtac aatggacatc ggtggagata
     4501 aagaactacc atatctgcaa ttacctcatg aaatgaatcc attcttagga tatcgtgcat
     4561 tgcgtatcag tttatctgaa caaggcgacg aaatgttccg tacacaaatg cgtgcattgc
     4621 ttcgtgcatc tgttcatggt aacttacgta tcatgttccc tatggtagct acactcaaag
     4681 aattccgcgc agcaaaagcg atctttgaag aagaaaaaca aaaattgatc agtgaaggca
     4741 aggaagtatc tgatacgatc caagtcggca tcatgatcga gattccagca gcagcagtct
     4801 tagcagacaa gtttgcaaaa gaagtagatt tcttctctgt aggtacgaac gatttgattc
     4861 aatacacaat ggcagcagac cgtatgaacg aacgcgtttc ttacttgtac caaccatata
     4921 acccatcaat tttacgtttg atcaaaaatg taattgatgc agcacacgct gaaggaaaat
     4981 gggcaggtat gtgtggagaa atggctggag atcaaacagc tgttccattg ctagtaggta
     5041 tgggtctaga tgagttctca atgagtgcga catctattct taaaacgcgt agtttaatga
     5101 aacgcttgga cacagcgaaa atggctgaac ttgctgatcg tgcattgaaa gaatgcgata
     5161 cgatggaaga agtcgttgaa cttgttcatg aatatgtaaa ataaacaacg tatcaaaagc
     5221 tggtgtggct tgtaaaagag ctacgccagt ttttttgttc ttttttaaga aaacgttttt
     5281 gtcagtaaga atagataaaa atagggctta agtttgagtg acagtttgta ggctatgtta
     5341 tactatataa gtgaaggggg acgtaaggtg aaggtattat tatattttga aagtgaaaag
     5401 atgcttgcaa aatctggaat cgggcgtgcg cttgatcatc aaaaacgtgc tctgacggaa
     5461 gtcggtatct cttatacact agatgaaaaa gaagattatg acatcctgca catcaataca
     5521 tacggaatca atagtcataa tatggtcaat aaagccagaa gagaaggaaa aaaagttgtc
     5581 tatcatgcgc attctacaga ggaagatttc cgaaattcct ttatcggatc gaaccagctt
     5641 tctcctatcg taaaaaaata tctggtagga ttgtaccaaa aagcggacta tctgatcacg
     5701 ccaacacctt attcaaaaca gttgttggaa agctatagta tccgtgtgcc gattcaagct
     5761 atttccaatg ggatcgactt agagaaatat cgccctgatc caatgaaaga acaaaagttc
     5821 agagaatatt tcaaactgtc tccggatcaa aaagtaatca tttgtgtcgg tttgtttttc
     5881 gaacgtaaag gaatcattga tttcgtagaa attgctaaga agatgcccga atacacattt
     5941 atttggtttg gtcatgttcc catgtactcg attcctcgga acatccggaa aattgtaaaa
     6001 gaagatcatc ctgaaaatgt cctgtttcct ggttacattc gtggagagat catagaaggt
     6061 gcgtattcag gtgcagatct attctttttc ccttcttatg aagagacaga agggatcgtt
     6121 gttttagaag ctttagcaag caaacaaaac gtacttgttc gagatattcc agtgtataat
     6181 ggctggttag aagatagcaa gaactgctat atggggaaga caaacgaaga atttatccga
     6241 ctgatccaac agattaccaa tcaagaacta ccgagtacga cacaagccgg ttatcaaaca
     6301 gcaaaagagc gaagtatcaa taaaatcggt gaagaactta aaaatgtata cgaaagtgta
     6361 ctgaatgaaa aagtctctag caaattaaaa aaagtaaatc aggtcaagga ctagtttgga
     6421 ggcgaaaaac atgaaaatcg gcttttttac cgatacctac tttccgcaag tcagcggagt
     6481 tgctacatcg atcaaaacat taaaacaaga attagaaaga catgggcatg aagtctatat
     6541 ttttacaacg accgatccta atgcggataa gttggaaaaa gatgtgattc gaatgccaag
     6601 tgtaccattt atttctttca aggatcgaag agttgttgtt agaggcatgt ggtatgctta
     6661 tttggttgcc aaagaactag aactagatct tattcatacc catacagaat tcggagcagg
     6721 gatacttggg aagatggtag ctaagaagtt gaaaatacct ctgatccata cttaccatac
     6781 gatgtatgaa gactaccttc attatatcgg taaaggaaaa gtgatcaggc aatcccatgt
     6841 aaaatacttg tctagattat ttgcaaatca tacgacaggt gtagtttgtc caagtgaacg
     6901 tgttattgat acattacgta gctatggtgt aatagcaccg cttcgcgtga ttcctactgg
     6961 tatcgacatt gaaaaattca aacgttctga tatcactcag caggatatca gcaaattaag
     7021 ggagtcacta ggtcttcagg aaaaccattt gatgattctt tctttaagca ggatttctta
     7081 tgaaaaaaat atccaagcct tgattaatgg gtttcctcag gtcatctccg cttatcctaa
     7141 cgccagatta gttatcgtag gaaaaggacc ttatgtagaa gagttagagg aattgatctc
     7201 agaactgcag ttagaagaat ttgtgcagtt cacaggagaa gtggacaaca atgatgtagc
     7261 actttattat aaagcggcag attattttgt cagtgcttct acttcagaaa cacaagggct
     7321 tacttataca gaagcgatgg cagccggaac accttgtgtc gtggaaggaa atgcttattt
     7381 gaaccgattg ttcgatcatc cttcattagg gaaaactttt gaaaccgacg aagactttgc
     7441 accagcactt attgattata tttccagtga agttcctaaa gatccaaaag ttttagaagc
     7501 taaactttat gatatttcat ctacacattt cggcgaagct atgatcgaat tttatcaaga
     7561 catggttgtt tatcatgaag aagagatgcg aagaaaagaa gaagaagact cgattgaacg
     7621 aatcaaaata aaattaaatt catttaaaag ataaaattca aacatttttt atgaaaaaat
     7681 ccttgtttct agcgtatact agcatagaag caaggatttt tgatttttag gaggtcgaaa
     7741 aaatgaaact tggttttatc ggaacaggcg tcatgggaag tgctgtcgca cgccatctac
     7801 tagaagcagg acacgaagta gctgtctata atcggacaaa agcaaaagct gatcctttag
     7861 taacagaagg agctatctgg gcagatacac caaaagcggt agcagaacaa agtaatattc
     7921 tctttacaat ggtaggttac cccaaagatg tagaagaaat ctactatgga cagtcgggga
     7981 ttttttctgc agatatctca ggacatattt tagttgactt gactacaagt acaccaagcc
     8041 tggcagaaaa aattgcaaaa acagcaaaag aaaaaggcgc agatgcgtta gatgcaccgg
     8101 tatccggtgg tgacttagga gcaaagaatg gtacactgac gatcatggtc ggtggagaag
     8161 aagccgttta tgatcaagta ctgcctttat tcaaagaatt tggtacgaca tttacattgc
     8221 atggttctgc tgggaaaggt cagcatacaa aaatggctaa tcagatcatg attgcaggta
     8281 cgatgacagg aatgacggaa atgcttgtct atgcacaaaa aaacggactt gatttaaaga
     8341 aagtgattga gacgctttca ggtggaagtg cagctaactg gtctttaagc aattattctc
     8401 ctcgtatttt aaaagaagat tacacacctg gtttttttgt caaacatttt atcaaagatt
     8461 taaagattgc attagaagaa gcagaaaaaa tggaccttgt attgccagca actacccaag
     8521 cattgaaact atatgaagag ttggcagata aaggttttga gaatgacgga acccaagctt
     8581 tgatcaaact ttggtggcct gaaggaaaaa taccagaaaa aaaatcataa aaaacgagtt
     8641 tttttagaga aaatcaatgc aagtaggcta tgcttttgat acaatataac agaaataaga
     8701 aaaggaggac ctccgatgac acattcacaa ttagtcgcaa tcataaaacg actggaggct
     8761 atggtcgagt cagcagataa tgaactgcaa gttcgccgtt ttgaaaaaga aggcgtagag
     8821 aagtgtacag taacttatga taaagcgacc gatacatttg aattaactga aacagatact
     8881 catcagcaat acgaatttga taatatcgat atcgtagcga tggaaatcta tgacttaatc
     8941 caataaattt tagaataatt gacatttgaa gttgaagatg cctaaaagaa gccgagaagc
     9001 aactaattta gttgcttttc agcttctttt ttgtttaaca gttcaatttc agactgttct
     9061 ttttctaatt ttttgcggta actgatcaag atttgtacag gatcgatttc ttcttttgac
     9121 aattgacgtt tcaaagataa atgagcttct ttcttttcat aatagaagtc aggagaaacg
     9181 tcagcagagg caatcattgt ttctgcaagt tgaccataac aaacgaggta gtgctttttc
     9241 tgactctcat ctgctgctaa tggcatgctg aaggtgagcg atggtgttct taaaaaatgc
     9301 ttttgttcct ggatccgtct aataaaataa gtaactaatt cgatatcttt ttgaagcata
     9361 ccggcttttt cgaaattcaa ctgatagatg aaatgttgtt gtcttttttt caagtaagtt
     9421 agtagctttt tattttggcc ttgaaacagc ccttggagtt cctttttttt ctgttcaaaa
     9481 gtcatttcct gtaggtcagg aagctgggct cgcaaagcta acaaactaat ttcagacaac
     9541 caaggcattt gataagtttc ttccattata cgcaaaatag acggcatttt tttatagaga
     9601 cgaaaaggcc cgtatgttcg agccgtagga gtatctgtta atacaaatc
//
LOCUS       DAXWLG010000099         9446 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXWLG010000099 DAXWLG010000000
VERSION     DAXWLG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.100"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            48..917
                     /locus_tag="ACGE4T_001903"
     CDS             48..917
                     /locus_tag="ACGE4T_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8387149.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1126..2151
                     /locus_tag="ACGE4T_001904"
     CDS             1126..2151
                     /locus_tag="ACGE4T_001904"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8387150.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2190..3140
                     /gene="manA"
                     /locus_tag="ACGE4T_001905"
     CDS             2190..3140
                     /gene="manA"
                     /locus_tag="ACGE4T_001905"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFF8387151.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3234..4937
                     /locus_tag="ACGE4T_001906"
     CDS             3234..4937
                     /locus_tag="ACGE4T_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8387152.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            4939..6972
                     /locus_tag="ACGE4T_001907"
     CDS             4939..6972
                     /locus_tag="ACGE4T_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFF8387153.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            6983..7423
                     /locus_tag="ACGE4T_001908"
     CDS             6983..7423
                     /locus_tag="ACGE4T_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8387154.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7623..8750)
                     /locus_tag="ACGE4T_001909"
     CDS             complement(7623..8750)
                     /locus_tag="ACGE4T_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8387155.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8753..9430)
                     /locus_tag="ACGE4T_001910"
     CDS             complement(8753..9430)
                     /locus_tag="ACGE4T_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8387156.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
BASE COUNT         3198 a         1642 c         1943 g         2663 t
ORIGIN      
        1 agaatccatc gttaaaataa agcttgacgt agaggggagc aagaaatatg aaagattttc
       61 aattgggggc tgccacctat ttaaaaagtg tagccattcg ggtgaaagac cgagataaaa
      121 tgattgattt ttacaagaac gttgttggct ttgatttgaa aagagaagaa aacgaattag
      181 ccattttagg aacagaagaa gctggaagtg aaatgttgct gctggaagaa agtcctagag
      241 cgaatgatca catgggggtg atcaaaaagc tgaaccgttt ttcattgatt atccctagca
      301 cagaagaaat ggcagatatc ctttgtcgta tccgtaaaat gaattatcca atcaaagaag
      361 ctttagaaga tcatggacgt atggggattt tgctcgtaga cccagaagag aatgaattgg
      421 aaatttacca tgaagagaat aagaacgacc aaagagaaat tccagaaaca atggatcaag
      481 aagcattatt aagaaaatct gaagaaaaat tcagtaaact atcaacaggt tcttattttg
      541 aaaaaatcca tttaaatgtt actaacttaa caaaagaacg ccaatttctt aatcatgtac
      601 ttggtttgaa ggtacataat gaagaagagg gcatccatgt attgaatgaa ggagatttcc
      661 atgttggttt gacagaagct agcggaggcg caattgattt gcctacagat gaagtgattg
      721 gacttgattt tctgaaattc atgatcgata ctgaaagcat ggaaggactg attgctcact
      781 tgcagcaact atcagttgat ttcttcttag acaaaaagca cacagtactc acaatctatg
      841 atgctatagg aatcgaatgg tggttcgtca gtgagccaaa agaaaagaat aaaactgaca
      901 ttcgatgcga cgcttaactt tttctttctt aacgattgtc gcatgtctcg tcattttatg
      961 aacggtcata ctatcgctta ttctagagat agaaagagaa aatagtatac cgggggaaac
     1021 tgcgcggttt ttcgcgcagt ttttttgtgt aaaaaaggtt gattcctcat agattaagct
     1081 ttaagcttgg actgtaagaa tctgatagaa aagcaggaga aaaagatgta tccacacacg
     1141 aaacagctta ttttgaaaaa gatggaagaa ggattttatc caggtgtagc agtcgccttc
     1201 ataaaagaaa atcaagtaga aacatttaca gccggctttg cccaagtcgt tcctgaaaag
     1261 cagctgatga cagaagatct gttgtttgat gcagcctcac tgacgaaagt cgttgcaacc
     1321 acttcattag ttttacgtct attagaagat ggaaagatag aactcgatca accgcttcat
     1381 aaatatcttc ctgaatttga aaatcagaca atcactttgc gtcatttact gacacatact
     1441 tctgatatca aaacatggat accaaacaga gataagctat ctcaggaaga attgatcgcc
     1501 gcttatttga agctagaacc aggtgagcaa ctaggaaaag aagtaaaata tacagatgcc
     1561 ggtacgattc ttcttgggtt gatgctagag gaaatttacc aagaaactgc agtggaaatc
     1621 tttcaagaac aagtattgga accattagga atgatgaaca gcttattcct tccttttcct
     1681 tccaaaaaaa tcgttcccac agaacaactg ccttcgggtg aggttttgcg tggacaaact
     1741 catgatccga aagcaagagt tttggctgaa catgcaggta acgcgggtct ctttattgat
     1801 ttgaaagaca gcattacttt tgtccaaatg tatttgaaca agggaaaaat ccgtaatggt
     1861 cgttttttag aagaagagac gattgattct ttactgcaag atcaaacacc gaaaaaaaat
     1921 ggctatcgtt ctttaggatg ggacttaaaa aaggatgtag aagacggtca tgcgattctt
     1981 tttcatactg gttatacagg tacgtttttc ttgattgata taaaaacaca agaagcgttg
     2041 atatttttgt caaatcgcgt acatcctgtc gatcatcgtg cggagtatct catatgtcga
     2101 gatgaaattg ttgaaaactt tttgaaagag aaagcggtat taccgaaata atgctataat
     2161 agctatgaaa aatgagagga gtttgaatta tgcaggaacc tatgttttta aaaccagttt
     2221 ttcaagaaaa aatttgggga ggaagtcgtt tacgttcagt ctttggtttt gatatcccta
     2281 acgataaaat cggagaagat tgggcgatca gtgcgcatcc tcatggtgtc agtgtagtag
     2341 aaaatggcga attcaaaggg aaaagattag atgaactatg gtcagaacac aaggaattgt
     2401 ttggccatcc ttcagaacca gtgtttccat tattgatcaa gatcttagac gcagaagatg
     2461 atctatctgt acaagtacat ccagatgatg cctatgggat gaaacatgaa ggtgaattag
     2521 gaaaaactga atgctggtat atcatcgatg ctgaacctgg tgcagaaatc atctatggtc
     2581 atcatgcaaa gacaagagaa gaactagctg agatgatcaa agatgggcgc tgggatgacc
     2641 ttttgaagaa agtaccagtt aaaaaaggtg atttctttta tgtaccaagc ggaacgatcc
     2701 atgctattgg taaaggaatc atgattttag aaacacagca aagttctgac acgacttacc
     2761 gcgtgtatga ctacgacaga aaagatgcgc aaggacaaac aagagagctg catatccaac
     2821 aatcgatcga tgtaacaact gtccctgcga aaacaccaga attacaaatc aaagaagtcc
     2881 gaaaagggaa ttcatcgatc gtcacttatt tagaaactga gtttttcaat gtatatgagt
     2941 gggatattaa aggaatcaca tctttcaaaa aacaagcccc ttatacattg atgacggtaa
     3001 tcgaaggagc aggagacctt gtcgttgacg gaaaaacata tccattagaa aaaggaacaa
     3061 gctgtattat tccaaatggc gtttctgagt gggcggtcca aggtgaacta gcaatcattg
     3121 cgtctgaacc aggcaaatag ttcccagata gctgaaagca gcagacggtt tttcgtttgc
     3181 tgctttttct acataaaatc cgtccatcaa taaatagaag tctggtgaaa aagatggaat
     3241 acaaatggaa agcaaacaac agcagtcgcg tgtctatgaa acaactgaga aaagctggac
     3301 atcacttaaa tgaaatgatc atgccaaatc taagtctgtt tatcgcttgg ggaatcttgt
     3361 cattagcaac aaaaggtgtg tctggcgatt tgcacaccac attagaagag gtatgccatt
     3421 ggatgatcca gctgctttta cccatcttga tcagttattc aggcgggaaa tcggtagggg
     3481 gacaaaaagg ggcagtcgcc ggtgcaattg cttctttagg gttgatcgct cgttccaatg
     3541 ctccacaaat cgtaggagcg atgattatcg ggccgcttgc cggcatcgta tatgaacaat
     3601 ttgtttctag attccatgtg aaattcaagg ctggatatga aatgcttgtc agcaacctac
     3661 tagtaggact gactggtagc gttatttgta taattggaat cgtttttatt gaaccagttt
     3721 taaatagtgt tcacttgttg ctcgcctccg ttgtgtcttg gctagtcaca atgaatctgt
     3781 taccacttgt cagtctgatc ttagaacctc taaaagtact atttttcaat aatgcaatca
     3841 atcatggtgt attgacacct ttaggaatcg aattctcaca aacagtcggg tactcttcct
     3901 tatttttgat ggaggcaaat cctggacctg gtttagggat cttactttct atcctttgtt
     3961 ttgcagaaaa acaggaaaaa gtaaatgcga gtggagcatt gatgatccat gcgattggcg
     4021 ggattcacga aatctatttt ccatttgtct tgctgcgacc gtatctattt ttagctgtaa
     4081 tggctggtgg ggcaagcggc acgttgatat ttcaatggtt agatacggga ttagctgcac
     4141 ctgtatcgcc tgggtcacta ctcgtgattt ttgccaatgc gccttatcca caactagctg
     4201 gggtaagtat agggatagca atcagtacat ttgtgacctt catttgtgca gcgatactat
     4261 tgaaattacc aaataaagaa aaagagaaca aaacggtcaa agaaagggga gaaatcttgg
     4321 aaccatctat cattcattcc gtcatttttg cttgcgactc aggtatggga tcaagtgcta
     4381 tgggagccgc cctgctgaaa aagaaattag cagcagaaaa aatcgatctt tctgtcggat
     4441 acagctctat ctatgaattg aataaccaag taaatcagct ggttatcgtg caacatgaac
     4501 tagcgggtct tgcaagagaa aaagtacctc aaagtcaaat ctatgtactg gatcactttt
     4561 tggaaacaga tatcttgctg tcacgaataa aagaagcgat ccaatttcca aagcgggaag
     4621 aaaaaaaaga gatagcagat atcacagaac aaaaaacgtc gcctgctaat attgtatttt
     4681 tgtataaaaa caacagacgc ggctcacaaa ctatgggcat gacgatttta agtcaattag
     4741 caaaaaaagc gaaaaaacag tttgttatct caaaagaatc aatagaaaaa atcaaaatcc
     4801 aagaagaaac gatttatatc gcacaaagag aactagctgt aactgaacag ctagaagaaa
     4861 agatccctcg gctagttcta gttgaacatt gggtagctac gaatgaatac gaaaaactcg
     4921 tgaagggaga aatataagat gtatttatcg cccagagaaa aacaattatt agcagaattt
     4981 ctcagtagcc cgtcacctgt atctatccaa aaaatgatga atttattgaa ggtcagtaaa
     5041 cggactattt accgcgaatt agataatctt gaagtctcat tgaaatcggt aaatgcttct
     5101 ttgaaaaaag cagctagagg ctcatttgtc attgaggcat cagaagaagt attagagcaa
     5161 ttaaaagaaa atacagatgg gaaaagtaat gacttatcca ctgctaaacg acaacacgcg
     5221 attttagccg agttgttact gacgacagag ccgttgagtc ttgctcattt catggaatca
     5281 tatcgcatca gtaataccac tttttatgca gatatcaaac agttggaaga aagtattcgc
     5341 caactgcctt tggaaatcat tcggaaccag ggatacgaga tcgaaggacc ggaaaaatac
     5401 cgccggcttc tgacagcgaa tgtattagaa ttagaaatca atgaatatga actttttcat
     5461 agtatttctt tcgatgcatc actgaattat ttttttcgat tcgttgatcc gcaacatcta
     5521 tctcttgcac gcaaagtaat cggggaagaa ttgatccaaa aaaagaccaa tctaagtgat
     5581 agaaagcttg aacatttggt tttgatgctg acattgacga tggagcgtgt gacgaaaaat
     5641 catttactga caaatgagac atataatagt ctggcaaaca aagaattact acagacagct
     5701 aaacgattgt tttcacggat cgcttctgag acaaaacaac tttatccagt aaatgagatc
     5761 gtcttttttg caggattatt aaatgacttc acgaactcat ttgatcagaa tttctttgaa
     5821 gagacgttcg atacacagct tgcttattta gtaaaacaac tgattcaaga agtaagtgaa
     5881 gagacgaatg tccattttta tgaagatgaa actttgtaca aaatgctgtt gactcattta
     5941 tctggtgtgt tttcaagagc cgttttacaa gaagagacac tgaccaatcc gattttagaa
     6001 aaaatcatgg ttcaatacga ggaagttgcg aatgcaatcc gtaaagcagt agagcggatt
     6061 tttcaagaaa aaaaattatc cgaagaagag atcgcttata tggtccttca ttttgcgaat
     6121 tcattggaac gaagtccgaa agaaagcaat atcagtattg caggattttc gccaagtgga
     6181 ttagcttcta ctagcatgtt ggaactacga ttgaaaaggt attttccctt catccaacaa
     6241 attcatttct atcggattgc tgatttaaaa aaaatcgatt tacaagaaga atatgatgtt
     6301 gttatttcta catctatttt accaggatac ggtggaaaat atattttagt ctccccgcta
     6361 ctgctagaag atgaagtcaa gcagctaaaa gaagcttttt cgcatattga gaagaaaaaa
     6421 aggcaaaaaa ttacgtataa cagcaataac gagtggatga tagaagatac ttatgaggaa
     6481 acaatgcatt tcatggatca aatcaatcaa ttactcggtc gtttttttat ccaagaactg
     6541 gataatcctg agacgatttc cgaaacgatt cgcagcttat ttccttattt tcctgaagga
     6601 ctagtaacgg atgaagaggt ggttcatcgc cggctgctta agcgatatga acaagcacct
     6661 atcggtattc ctcatacaaa tatggggtta ttccatacat ccagctcatt gatttctaaa
     6721 ccgatctttt gtattttcaa tttgaaacat ccgttgacca ttgaagggat ggatagacag
     6781 ccagtcgatt tgtcccgtat gttagttatg ctggcgcctt cgccaatcca agaaaaagac
     6841 gggattcttt taggaaagat cagtggagca atcatcatga atgacttgaa caccgagatc
     6901 ttccgctcag gaaataaaga aatcgtttat cagttgttag cgaaaatcct gattgaagaa
     6961 ataaagaaat gaaggtgaaa agatgataca gctaccagca gaaaaaatat tgctcgatca
     7021 atcctatcaa aattggaaag aagcgttgac cgagatcggc cagtggatga cccaacaagg
     7081 gggaatcaaa ccggcatata tccaatcaat gatcgaaaga caagaaaaag cgagtgtcta
     7141 tatcgggaat ttcgtagcct tgccacatgg gactgacaaa gatgaagaag tagtagaaga
     7201 aggaatctat ttagtccaag tgcctgatgg tgtggacttt ggaacaaaag aggaaccaaa
     7261 aatcgcgaca ctcttatttg tagtggcttt aaaaaaagaa catcagttag cggtgctcca
     7321 agatttagcc tttttctgtt cagacatcga ccaagtaatg gcgctcagtg atgcggaaac
     7381 cattgaagaa gtccaaatga tttttgaaca tggacatata taaccaatgt tcacagtaaa
     7441 aaaagctgcc ttgcatcaag taataacttc aaccaagata aaaataagag gagctatttt
     7501 tgttgggagg ttcttattac tgcggatgca agcttttgaa aactatttat tctagaaaac
     7561 cgtttatttg aaatagggga cggtgacaag gtttttgtca ccgcccctat tatttttgtg
     7621 ttttaatagc tcttttttat atagatagtg cccataccta tgaataataa aacgatactc
     7681 catataccaa gtgtcaagat accattcatg aaagtaacct gttctgaagc tagtggcgag
     7741 aaaatatctc ctcctatgat cacattttga aaatcaacat aagaagttgg caagtaatga
     7801 ctgattgatt ctatccatcc aatcgaagaa aacaagcgat actctgctaa gaaaagacaa
     7861 agaagtgtac ctaaaagatt gactagagaa tgttcaaata aagcagatag aagaaaactg
     7921 attgtactta aaaagagcac ccacaacaac aggaacagaa agctctggaa aagatactca
     7981 tacatcggca ggattcgtat ctcctgacca atgatcgttc ctacaggata acggaagtct
     8041 cccaaaccat ttttgattat tactagtata gagacgatca aaaatggcat cacgagactt
     8101 agcatagctg caccataata aatggtaaaa cggatgctat gtttctttac catactatcc
     8161 ggtaatagat tgactagaga gattacctgc tttctttttt cataagagat gatggaagaa
     8221 agaaacaagg cggttataca taaaatcaaa aaactagaaa tcattcctga aaaaatattt
     8281 tcgtagtaat ttgctaaaga gagatgcgct ggcaagtcaa aaatagaata tctttggatc
     8341 tgatgttctt tcatataacg gagcaactcg acacgttttt gttgttcaat gattggaatt
     8401 ccttgtcttt gaccagaaat cagtcgatcc agtatatctt tttcataatg ataagttgct
     8461 tcgaccattt gctggacatt ccctgtgttt tttgcatgta agattgcttc gatcagtttg
     8521 ttgcttttct ctaaactttc cacaaattct ttctcattct catcttcttt caacgtttca
     8581 atcaaacgtt gcatctcttc atagttatga ttcagttcat cgaattctgt ataagcaagt
     8641 ttatgatcaa aaaaaagtgg gaatgcaaag agcacgacca acaatagagg aaataaaaag
     8701 tttactttgt tttttagtaa gagattgcgt tcaaatgtaa gtaaagacat agttacacct
     8761 catataattt acgataaata gctaaaatct cttccatatt tgaggattct gtgacgattt
     8821 ctccattttt tagaaaacat gctttagaac aaatcgattg gatattcgcg atagcgtggg
     8881 aagaaagaag aatcgtcttc ccctctttct gccattgtct gatgaggcta ttcaagatat
     8941 caatacttcc tgggtctaat ccgttcaacg gttcatcgaa taacatgaca gatgaatcac
     9001 tgatcaagta agcagcaagc aagacatgtt gtttcattcc tagagacatt tttccaatcg
     9061 gcaaagtaag gtactcagtc atcttcaatc gttggaaaac agtttggcat tctactttgc
     9121 tctgccaata gtatttgatc aaagtcaaat aatcaacagc cgatagatag tcggataact
     9181 gattcgttga ttctagaaaa aagaaagacc ggcgataatc tgtataagca gtaggagaga
     9241 tgttgtgaag ggacaattga tcataagttg ttggcagcag ttgggcaatg ccattcagca
     9301 aagttgtttt gccaaatccg ttcggggcaa tcaaagcagc tacttctcct gattctacta
     9361 aaagattcaa ttgtcgaatc acacgttttt taccacgttt catgttcatg tttttgatca
     9421 gtaatgtcat agataggcct ccaata
//
LOCUS       DAXWLG010000100         9354 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXWLG010000100 DAXWLG010000000
VERSION     DAXWLG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.101"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(157..1206)
                     /gene="recA"
                     /locus_tag="ACGE4T_001911"
     CDS             complement(157..1206)
                     /gene="recA"
                     /locus_tag="ACGE4T_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFF8387157.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1315..2556)
                     /locus_tag="ACGE4T_001912"
     CDS             complement(1315..2556)
                     /locus_tag="ACGE4T_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFF8387158.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRNELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2796..3374)
                     /gene="pgsA"
                     /locus_tag="ACGE4T_001913"
     CDS             complement(2796..3374)
                     /gene="pgsA"
                     /locus_tag="ACGE4T_001913"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFF8387159.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3583..4506)
                     /locus_tag="ACGE4T_001914"
     CDS             complement(3583..4506)
                     /locus_tag="ACGE4T_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFF8387160.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5245..6531)
                     /locus_tag="ACGE4T_001915"
     CDS             complement(5245..6531)
                     /locus_tag="ACGE4T_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8387161.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6524..7792)
                     /locus_tag="ACGE4T_001916"
     CDS             complement(6524..7792)
                     /locus_tag="ACGE4T_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8387162.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8044..8505
                     /gene="mscL"
                     /locus_tag="ACGE4T_001917"
     CDS             8044..8505
                     /gene="mscL"
                     /locus_tag="ACGE4T_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFF8387163.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8564..9010)
                     /locus_tag="ACGE4T_001918"
     CDS             complement(8564..9010)
                     /locus_tag="ACGE4T_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8387164.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9103..9348)
                     /locus_tag="ACGE4T_001919"
     CDS             complement(9103..9348)
                     /locus_tag="ACGE4T_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFF8387165.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         2579 a         2018 c         1664 g         3093 t
ORIGIN      
        1 ttctattaaa ttattctttg tagatgaaca gatattttta ttttatgtac ccactcgtat
       61 aattaaataa ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag gagagtacct
      121 actcttcaga ctagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc
      181 ttcttgtcct ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct
      241 aactagagca gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt
      301 ttcccgtcct tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac
      361 aatgtctttt tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata
      421 catgacatct acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat
      481 ctttgtacgg ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc
      541 caaacggatc gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattccc
      601 aaacatcacg cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt
      661 gttgatcgaa ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac
      721 atgtgagtca cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc
      781 gactactacg atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg
      841 ttctcctgtg tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc
      901 atattgcgga tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg
      961 tacttctgca atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac
     1021 ttcgatgata cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga
     1081 gccacttgga attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc
     1141 tttaccatag ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc
     1201 tgccaatacg ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc
     1261 atatcttttt tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt
     1321 tcttaaaagc tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc
     1381 agcacgatct ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc
     1441 gatccatact gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa
     1501 agctacactg taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca
     1561 ttctttgctg actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc
     1621 ttttgtttgg agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat
     1681 gtcgcctaat gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg
     1741 cttgttttct ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc
     1801 ataaaaatat tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt
     1861 ccctgcctga acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc
     1921 atatggtgcg atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag
     1981 tcgcgattca ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg
     2041 aaaacgttcc tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg
     2101 cggaccggga agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag
     2161 ccctgtacga tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt
     2221 tttggtcatt tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt
     2281 attttgtatg agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc
     2341 tgtcggtcct aatccgccac ataagagtat gagttcattt cttgtttcag ctagagcaat
     2401 caactcttct aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc
     2461 tagatcagct agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa
     2521 aagttctgtt ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta
     2581 tacgctgtat ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct
     2641 tgtcacagct ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc
     2701 acaatataca taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta
     2761 gacggcaggc tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg
     2821 ctgtttttga tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc
     2881 atgatttgat ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag
     2941 ataatagcaa ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact
     3001 tctccatgtt cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg
     3061 atcgctacta cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata
     3121 actagcattt tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc
     3181 gcacgggcaa tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca
     3241 cctactagct gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa
     3301 ggtaaaccta caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta
     3361 tttggtaaat tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt
     3421 ttccaccgat attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa
     3481 ttgatctttt tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt
     3541 ttatacctac aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag
     3601 agtaaggttc acattttttg gactctgatt gttttcccct ggattaactg gtacggattc
     3661 tccattcgct ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc
     3721 tgtttccggt aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata
     3781 ggcattgtct actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac
     3841 aggcttggta gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc
     3901 tattgccatt ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt
     3961 ggtctgcgtg ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga
     4021 agaagctgtg tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac
     4081 gacaacgaca atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa
     4141 gcgacttgga tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg
     4201 ctgcggacgt tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa
     4261 atggtctcca tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa
     4321 tttaccaggc aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat
     4381 ttttgtaatc tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa
     4441 tttttctccg atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg
     4501 aagcatctgc tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca
     4561 cagctccttt tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa
     4621 tatccaataa atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg
     4681 gcaggctcac gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca
     4741 aaaaccggca aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa
     4801 cgctgatttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
     4861 tccaaccaag ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg
     4921 cagggcagct ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa
     4981 taagccgaaa tatcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
     5041 aagctgacca cttctgtcac aacctctttg attacttcgt ttgattttat tttaatataa
     5101 gttgattaaa agagagcatg atttgtttta ttttctcact aatttttcca acacaactaa
     5161 gaataccata aaagctgatt ttggaggagg ttcctactac aagtctaaaa cgaaatttaa
     5221 gacttttcct cataaaatac tggtttattt tggtctcatg taaaactcgc tgaaggcctc
     5281 ttcacttata aatgcttccc cagctgccag tacgtctttc atttggatcg aatcgatgat
     5341 ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt tgcgtaaatt gattggcaat
     5401 atattcgatc gaatttaatg attggaaata ttgtccaagc attttcttct tcagcaaatc
     5461 gagatttgtt tctgacactt ccggatcatc agcgaatcca agaatgattt ctttgacttt
     5521 ttctgctgct ttctctggtt cgtccgtatc tccgctgaag tccgcaaagt gaaattcacg
     5581 atccaagctg aattcaaaac caaagctgtc atcgataatt ccttgattgt acatcgctag
     5641 atagtttcga gaagtattgc ctaaaatcat ttggaacaac agattgattg ctgttttata
     5701 gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat cctttaatac caagtacaaa
     5761 tttatctcgt gtaatggctg cttccaatgc actttgtttg atgatttctt ttgtgttttc
     5821 agggaaataa cgtacaattt cttgctttgg agggaaattt tttgcttctt ggttttcccg
     5881 aataagtttc atcaatttct caggctccat cttaccaact acaaataaaa ccatattgct
     5941 tggctgataa aacgtccgat agcacgtata caagtcttga gctgtgattt tgtcgatact
     6001 ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta ggatacaagt tatttagtat
     6061 gccaaaaaac atccgccagt tagggtcgtc ttcatacatt tggatttcct gaccgatgat
     6121 tcctttttcc ttgtttacag tttcttctgt gaagtaaggt gcttgtacaa aatcgatcag
     6181 agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac agatagctgg ttttcgtaaa
     6241 acttgtgaaa gcattggcgg aagctccttg tttcccaaat aattgaaaga catcaccatc
     6301 ttctttttca aacaacttat gttctaaaaa atgggcaatt ccatctggaa ctttcacctt
     6361 ttctttttca ccatagggaa taaattcgtt atcgatcgaa ccataattcg tactgaacaa
     6421 accatatgtc ttgtgatagt catttttagg caacaaatac acggtcaaac cgtttggcag
     6481 tacttcatga taaagtgttt cattgatttg ctcatattct gttttattca tttgcatttc
     6541 ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc agctacttgt tgtatctctt
     6601 tgatcgttac gctctccata cgtttcaacc attcttcatc agataatttt gtttccggca
     6661 gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg agagtctaga gaaagcagat
     6721 attggtttct cagcattgct tttgtctgag acagttccaa ctctgttatt tcaccatttc
     6781 gcaaactttc taattgctcg tgaatcagat gaagtacttg atttcggttc ttttcatcga
     6841 taccggtttg gacactcatg aatcctcgga acgtatccac gctactagag gcataataag
     6901 ctagactttc tttctctctg acattcatga aaagtttaga gtgaggaaaa ccgccaaata
     6961 aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc gtaatacaca tttgtctgat
     7021 aagctaggtt cagctttgct tgaacgattg gctcacgtac ttgtcgttcc ttcacgatat
     7081 tcacttgagg ttgggtataa aacagatctg gataaactgt ttcccgcgac tcaaatggta
     7141 attgcccaac tgcttcagct gctttgtccg gatcaacatc gccgacaaca aaaatgtcga
     7201 tttgatcatt tgccatcatt tcttgatagt aggacgctag cgaactagca gtgatcttgt
     7261 ccaaatcttc gactacgccc aaccctggga tcttttgttc cgggctgttt tcaaaataaa
     7321 gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc cttcaaactt tccaaatata
     7381 gacgcagatt gttcttttcc aagtcaaacg tcaactgatc aaactgatga tttttaatat
     7441 ttggataaaa taaaacctct tttaaaaaat ctaccgcttg agaaaaaagg tttggatcat
     7501 tgacatattc accattcaca aaactgatcc ctgcgtttag ccaatgtaaa tttccttttt
     7561 ttctaactga taatccaaag cttgcgccat aaagctctgc aagttgcgca cttaactttg
     7621 tctgatccgg ataattcaaa ctatttgttt ccaacatgct gctgagaagt gaacgtttcg
     7681 tgatgacttc ttgttgcaga cgcgcagtaa aacggataaa aatccggatc gttttgtatt
     7741 gttttgttgg taagacatga agattcacgc cttctgccaa ttgatacacc atatttatat
     7801 gctccttttc acagttgatt gcccgcttac tcttttaatg ggtcaacctg ccttttttac
     7861 ttattcctct gcttgaaatt tataagttca ggcaattcgc cgttttttct aaatgatgtt
     7921 acactttata gtatagcata gatgtttttt atcaaaagaa acagacggct aaaagcgtat
     7981 attgtttttt tattttttat ttttcgatat actaaagaca aataaaagaa gggagggtca
     8041 ttcatgatca aagaattcaa agagttcatc atgcgtggag atgtcttgga tcttgctgtc
     8101 ggcgttgtta ttggtagtgc gtttacaagc atcgtaaacc aagtggtgca aggcttgatc
     8161 acaccactaa taggttggtt tgtcgcaatg atcacaggta cttctgattt agaaggagca
     8221 ctttcaattc ttgactggtc gccgacaaaa ggcgtcacat ttgcgtttgg taacgttatt
     8281 agtgctatca ttacatttct gatcactggt tttgtcttat tcttagttgt caaagcggca
     8341 aaccgtgcga agaatttacg ctccaaagaa gagcaagtgg aagtggaaga aactcctact
     8401 gcagaagatt atcttagtga catacgcgat cttcttgcca aacaagtggc agaagacaat
     8461 acacatccag tagagcaaac aaatctttcc gatacttcta aataataaag aatctaaaaa
     8521 aaagaaagcc gattttcagt gtcggctttc tttttttaga ttcttaactt gctacttgtt
     8581 cctttttcat gtcgatatgt ggaatatcat cttccaaata cacatctgag attgccttga
     8641 atccaaacga ctcataaaaa gcttgtaggt attcttgtgc gccgatcacg attggttttt
     8701 ctggaaaatt tttctggatc atgttaatcg tttcttgaac aatctttctt cctaaaccat
     8761 ctttacggtt gtcctgatga acaaggacac gtccaaaatg gatactctcc gcttcttcat
     8821 atactcgcgt gtaggcaagg atcttcccat cagtatcttg aaaaaacgta tgccaagctt
     8881 gtccatcaat gtcatcaact tcttgataag gacactgttg ttccacaaca aaaactgcga
     8941 ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa ttcttcaaat ttctttacct
     9001 gatattccac taagcaaacg ccccctgctt ttttattctt agcataccga ttcgcacaca
     9061 aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct ttttaaactg cttcagttaa
     9121 aaattcgatc tcaactgtcc gagtaagtac atctgaatag ctgtaagata ctcgttcaaa
     9181 agaattttct tcaggatcca aatcaacaac gaatactgaa ggataggttt ctgttaaaac
     9241 gccgcggcgt tctgtttgac gctttcttcc tgtctgagca accaacatga ttgggctgcc
     9301 gatacgacct tctaaatctt tcttgattga agctaaagtt gttggcatac agtt
//
LOCUS       DAXWLG010000101         9343 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXWLG010000101 DAXWLG010000000
VERSION     DAXWLG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.102"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..135
                     /locus_tag="ACGE4T_001920"
     CDS             <1..135
                     /locus_tag="ACGE4T_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8387166.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            185..808
                     /locus_tag="ACGE4T_001921"
     CDS             185..808
                     /locus_tag="ACGE4T_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387167.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1258..2307
                     /gene="trpX"
                     /locus_tag="ACGE4T_001922"
     CDS             1258..2307
                     /gene="trpX"
                     /locus_tag="ACGE4T_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8387168.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2304..3185
                     /locus_tag="ACGE4T_001923"
     CDS             2304..3185
                     /locus_tag="ACGE4T_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8387169.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3188..3958
                     /locus_tag="ACGE4T_001924"
     CDS             3188..3958
                     /locus_tag="ACGE4T_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387170.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4237..5091
                     /gene="aroE"
                     /locus_tag="ACGE4T_001925"
     CDS             4237..5091
                     /gene="aroE"
                     /locus_tag="ACGE4T_001925"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFF8387171.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5269..6432
                     /locus_tag="ACGE4T_001926"
     CDS             5269..6432
                     /locus_tag="ACGE4T_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8387172.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6549..6959
                     /locus_tag="ACGE4T_001927"
     CDS             6549..6959
                     /locus_tag="ACGE4T_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8387173.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            7133..>9343
                     /gene="topB"
                     /locus_tag="ACGE4T_001928"
     CDS             7133..>9343
                     /gene="topB"
                     /locus_tag="ACGE4T_001928"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8387174.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSNLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNN"
BASE COUNT         3240 a         1602 c         2007 g         2494 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat ataataaaaa ataggagaaa aagtagaaaa
      181 gaaggtgttt gttatttggg aagtagttaa atcgagagta caagaaatga atgaacattt
      241 ctcagtgtca ttcgcagaaa atccaagtaa gtattttcga cgtcttgttt ttctgctcat
      301 cttatcattg ctgttaacag ttgcaatgtt tttattgccg gaaaaaatcc gagaagagca
      361 agtaaatagt ctcgtttatt cgtccatacc aaccagcaaa ttgaaacctt ttgattacag
      421 caaagctgat cagaaaatca aacaaagccg ggcgatatcg gtgatgttca gcaaaccgaa
      481 cgggaaaacc tatcaggaca tgcttgatgt ttttaatgat cctgagctta tggaagaatt
      541 gaatcgaccg atcttttatt atccgatcgt ttatgatgta catgaactgg aacaaaaata
      601 taacatacga acggatgaag tgacatttat tttctttgat ggaggaaaag aagcaaatcg
      661 gataactgcg ggtaagggag gaataactga ttttgataag gagttgatcc ctgaactgaa
      721 tcgacttccg ttagccaata tcaaacaatt agaagaagaa ttggatagaa cgggagtaac
      781 ttcccaaaat tccgagattg ctaattgaga cattggcgcg atgaaacggt gggtctatat
      841 agcgctattt ttaattatca gaacaagtga tgtttgatct tcaaaataac aaataaaaaa
      901 ttaaaaaata tagttgacga aattcaaaaa ttcgattaga cttaaatcaa ttcttaagga
      961 aagggtgacc tgtaatgaac ttatcaaaac gtatttcagc agaaaacaaa aaaaattggc
     1021 gcagctggcg tagataagtt gtattaatgg tgagactttc ccatagatac aactttttag
     1081 agtgcatttc taaagcttgt atctatgcta gacatgaaca ttgactgccg catagattag
     1141 aatctatgcg gttttttgtg taaagacaat gaccgctttg gccattgtct ttttttgtag
     1201 actggatact tgcctgatta taataaataa aaagattgac tttgcaggag gaaaaaaatg
     1261 aaaaacaaac gattaataac agttgtagta attattttag tttatttagc aggtgcattt
     1321 atttatggaa aagcctcagg cgaaaaagaa agtacttcga cgaaccagac aaaaaaaaca
     1381 acagttgttg gggtattgca atacgtgagc catccggcat tagatgagat ttatcgagga
     1441 atcaaagatg gtctgaaaga agaaggatta aaagaaggag aaaatcttga gatcaaattt
     1501 caaaatggac aagccgatca gagcaagcta gccacaatga gtcaacagct agtacaatct
     1561 gatccagatg ccttgattgg aatcgcaaca cctgccgctc aatcattagc aaacacgact
     1621 tcatcgatcc cagttgtgtt aggtgctgta acggatcctg tgggggctgg attagtcgct
     1681 tctttagaga aacctggtgg taatgtaaca ggtgtttctg atcagccgcc tgttacttca
     1741 caaatcaagc ttgcttctga tttgttgcca aatgcaaaaa aagtcggaat cttgtatgct
     1801 tcttcagagg ataattcaaa ataccaagta gcacaagcag aagaagctgc aaaaaaagaa
     1861 gggttagaat cagtaaaata tgcggtgcct tccactaatg agattgcaca aacggttcaa
     1921 gtgatgagcc gacaagtcga ttttatctat gttccattag ataataccat tgccaatgcg
     1981 atgcaaacag tggtaaaaga agcaaacaaa gcaaatatcc ccgtcattcc atctgtggat
     2041 acaatggtgg aacaaggtgg actagcaaca atcgggatca atcaatatca attagggctt
     2101 caaagcggta agatggcggc agaacttgcg agtggaaaag aaaaaccgga aacgacgcct
     2161 gtttatacat ttgatcaagg ggatacagtg atcaatcaat ctcaagctga tcgcttagga
     2221 atcattgttc cccaatcgat gaaggaaaaa gccaaaatca ttacagatga ctcgcagcaa
     2281 gaaacatcta aggaggatga caaatgatcg tatcagctat ttcacaaggc atgctttggg
     2341 caatattagg attagggatt ttcatgactt atcggatctt agactttcct gatatgacga
     2401 cagaagggtc cttcccatta ggtgctgctg tctgtgtgac agctattact catggggtag
     2461 cgccgattgt agctacatta ttaggcgtag gtgctggcat gatagcagga ctcgttacgg
     2521 ggttacttta tacaaaagga aagataccgg ttattttagc agggatttta gtgatgtcag
     2581 gattgaactc agttattttg tttgtgatgc gaacaccaaa tctttcttta ttgaatcatc
     2641 cgattctaca agacagcttc catcgctttg ggttacctga ttactttgac acagtccttc
     2701 ttggcttcat ctctttgggc atcttgattg ctgtactgct attcttcttc aatacagatt
     2761 tgggtcaagg ctatattgcg acaggagaca acgaaacgat ggctcggtcg ttagggataa
     2821 aaaccgatcg tatgaaaatt ttaggtttaa ctttgtcgaa cggtgtcatt gcattatccg
     2881 gtgcgctgat tgcccagaac gacggatatg cagatgtaaa taaagggatt ggcgtaatcg
     2941 tcatcggtct tgcttccatc atcatcggtg aagtgatctt ccaagaattg acgctagcag
     3001 aaagattgat tgctattgtt gtcggcagta tcatttatca gctattgatt ttagtagtaa
     3061 tcaaactagg gtttgacacg acttatctga agttgttctc cgctatcatc ttagctattt
     3121 gtctgatgat cccgcaattg aagaaagcac tgaacttaaa aacgggtttt gagaaggagg
     3181 catagtaatg actgatatat tgacaatcaa aaatggcaga aaagtcgtaa acaatggaat
     3241 gaatgaagag aaagtactat tggatgaaat taaccttaca atcagagaag gggacttcat
     3301 cactgtcctt ggcgggaatg gtgctggaaa aagcacactg ttcaatacga tcgctggtac
     3361 gcttcaactc agtcaaggat cgatctcttt taaagatcag ctgatcacaa aagattcaga
     3421 agaaaaaaga gcacgattcc tttctcgagt tttccaagac cccaagatgg gaacggctcc
     3481 tcggatgaca gtagcagaaa atttgctact tgcacaaaaa agaggtcaaa aacggaaatt
     3541 ccgtttacgt aacttgaaaa atcaaaaaga agattttttc agactatgtc aagaagtcgg
     3601 taatggccta gagcaacact tggatactcc agcgggtgaa ttatctggtg gtcaacgcca
     3661 agcattaagc ttgctgatgg cgacgatcac agctcctgag ttactattat tagatgaaca
     3721 tactgcagct ttagatccta aaacagctaa agcattaatg cgattaacaa atcagcggat
     3781 caatgaacaa aaattgactt gtctcatgat cacgcatcga atggaagacg ccttgaaata
     3841 cggaaatcgt ctgatcgtac tccaaaaagg gaaaatcgtt caagatattt ctgaaaaaga
     3901 aaagcaacat ttgacaatgg gcgacctttt gcagttcttt gaagaagtag tggattgatt
     3961 tcgacagaaa aattcctttc agtagaagga cttgaaaact cattgacatc ttcctttgat
     4021 tcatcctata cttaaaacaa taaacaatta aacatatttg ggtgttttgt aggatcacgt
     4081 agtcaatgat tttaacaatt aaatagaata ttctttgctc tgacaagtaa acgatgagaa
     4141 aagaatttgc tactttgcca cctagacgtt tcgtactttt tcgtatcaaa cgctaagtgg
     4201 ctttttttat tgaagtaaga acaaaggagg aataagatga tttctggaca aaccaaatta
     4261 gctgggctct ttgctgatcc ggcttctcac agtttatccc cattgatgca caatactgct
     4321 tttgaagcaa acaacatcga tgcggtgtat ttagctttcc aagtaaatcc aatgaatttg
     4381 aagcaagcag tcgaaagtat tcgaactttt gatatgttag gtgtaaatct ttcgatgccg
     4441 aataaaacag cggttattcc ttatctggat gaggtaagcc aggaagcgca attgatcgga
     4501 gcagtgaata cgatcgtgca cgaaaagggt cgtttgattg gctataatac cgatggtgcg
     4561 ggcttcatgc aatccgtgca agaagctggg atttcgatca aaaagaaaaa aatgactcta
     4621 ctgggagctg gaggtgcagc aaaggcagtc actgttcaag ctgcgttaga cggcgctgag
     4681 gaaatcgtag tttataaacg aaacaaccaa acattcgctc aagtaaaaga aacattcgaa
     4741 caactcagct atcgaacttc ttgtaagata aaagttcgtg actttgccga tacgcatcaa
     4801 ttaagaaaag acttgaaaga aagctgtctt ctagtcaatg caacagatat cgggatggga
     4861 aagaaaatca atcagtcacc gctcttagat ataagcatga tgcatgagaa tctagcggtt
     4921 tttgatttga tttatgcacc tagagaaaca cgtttattaa aagaagcaaa aaaagtagga
     4981 gcaaaaggat acaatggact tggcatgttg atctaccaag gagcaatcgc gtttgaatta
     5041 tggacaaagg aaaagatgcc gatccatgtg attgaaaagc tgtttgatta aaaaaggata
     5101 atagataaag atcttttatt ctcgcataaa cggtctcaag aaaagtaatc ctttatgtat
     5161 cgaagcattt atttcgttat tttttattaa attatcaaaa aatgataggc ttttgtcgat
     5221 tggttatcgt atcctttatt tatcagtaag aaacgaggag gaaccacaat gagtcggatg
     5281 gatcgctata aggacatcca taagaaggcg aaacctctga agcgggatac ggggagtatt
     5341 tttgctagaa gagaacgaaa acgaacgcgt atggaaaatg aggaagatgc tacccgtatt
     5401 tatgaaagag agcagcattc atttgaacat ttgaaccaat acgagcaaca gatatacaga
     5461 gatgcaccaa gagcagaaga acaacaaaaa aataaaaaat ttttcaatcg gcaaaagaag
     5521 aacaaaccgt taaaacctcg aagaaagcga tcgtggaaaa aaatcattct atcgattttg
     5581 cttgttttga tcctttattc aggtatctca ttttttattg gtaaatatgt agctgaacat
     5641 gacacaagct tagaggcagc gacttcagaa acctttcatg ggcaaaaagc gacgaatggt
     5701 gcgcataata ttttgatcct tggaagtgat acacgcggag aagatgccgg gcgagccgat
     5761 acgatcatgg tcttacagtt ggatggtcct tcccataagc cgaaactgat ttcttttatg
     5821 cgtgacagct tcgttacgat cccaggggta ggacaaaata agatcaattc agcttatgca
     5881 tacggaggag ccgatctagt gcgtcaaact ttagtagaaa attttggaat cgattgtcaa
     5941 tactatgcga aagtcgattt caaatcgttt gaaaaagtga tcgatgcctt attcatgaac
     6001 ggagtgaaga tcgatgcaga aaaagatctg aatctggatg gtgtagatat taaaaaaggg
     6061 gtccagaaga tggatggaca tgttcttttg cagtacgcac gtttccgaat ggatgaacaa
     6121 ggagactttg gccgtgtcag acgccaacag caagtaatga acgctatttt cagccaattg
     6181 aagaatccat tgaatctgat ccgtgcacct tatgcagctg gtaaagcttt aggatataca
     6241 tccacagatg tcactgcttg gttcttgacg aaaaacctct tttcgattgc acgaggagct
     6301 ggtggcattg atcgaataag tgtgccagta gacggttcgt ggaattatgg gcaaagtgat
     6361 tatgctggca gtattcttga aattgataat caaatgaatc aatctgctat tacagatttt
     6421 ttgaataaat gatcataaaa aagatcaaga agaagctgcg caaggctttt gtcatgaatc
     6481 aatcaaacga ttcactcgta aaaaacgctt tcttatggta gatttaacca agagaggagc
     6541 tgtttacatt ggatatctat ctgaacttca aaaatgagtc gcgtgaagcc atcgcttttt
     6601 atgaaaaagt ttttcagact gaagcatcag gtatcaccac atacggtgaa atgcctggaa
     6661 tagaacaaat gaacttgcca gaagaagcaa aaaatctaat cacgaatgca cggctgaaca
     6721 tttatgatac attagttatg tttgcagata cgccagaagg aattggtcct gaattagttg
     6781 tgggaaataa tgtttcttta gtcatccaga caagaaatga agaagaaacg aagcgcctat
     6841 ttgaaagctt agctgagggt ggtaagattg agatgccact ggatgctgtt ccgtgggctc
     6901 agttgtatgg gatggtaact gatcgatttg gcatcaactg gcagattaac caagagtgac
     6961 agctaaagat gaatattttc tggaaacatc aagcagcaaa cggatcatcg tttgtttgct
     7021 tgatgttttt gtgtctattt cgactatacg gtatcttttt tttattgcca gatgcgaaaa
     7081 tatgttccat aatagagaag tccgcaaaaa attaagtaac ggaggtcaga agatgaagac
     7141 ggtgatacta gctgaaaaac cttcacaagc aaaagcctat gccgaaacct tccaacagca
     7201 aaagcggcat gaagggtatt atgaaatcag cgatccattg tttgctggtg aagtaaccat
     7261 cacttatggt tttggacatt tggtagatat ggttgctcca ggagcttatg aagagcgttg
     7321 ggcaaaatgg tctttagaaa atttgcctat tttcccagaa accttccgtt acgaagttcc
     7381 aatggacaaa cgtgcacaat tttcgatcgt aaaacgagaa ttgcagtcag ccgataccat
     7441 cattattgca acagatggtg atcgagaagg tgaagccatc gcgtggtcga ttattcgaca
     7501 agcaaacgca ttttctaagg aaaagaacta tttacgttta tggatcaatt ctcttgaaaa
     7561 agaagcaatc tatgaaggtt ttaagaatct ccaacctgga aagaactatt ttccaaaata
     7621 taaagaagcg caagcaaggc agaacgctga ttggctgatt ggaatgaatg gaagtccatt
     7681 atatagcttg ctacttcaac gaaaagggat tgatggcagt ttttctcttg gtcgtgtaca
     7741 aacaccgaca ctctatatga tttatcagct gcaagaagaa atcaaacact tcaaaaaaga
     7801 aacgtacttt gaaggagaag gtttaattac gacaaacaaa gggcagtttt ctggaaaaat
     7861 cgtaccaaaa aaagcattta aaacccaaga agagttaacg aaggaaatag aaaaacttgg
     7921 agcgcattta ggtaagcaaa acggacggat tcttgaagtt acgaaaaagg aaaagaggtt
     7981 gcatagtcca cgtttatttt cgctatctaa tttgcagaca aagatgaatc agctaatgaa
     8041 agcaagtgcc aaagctacac ttgatgctgc tcaaggattg tacgaaagca agtttctaag
     8101 ctatccaaga acggattcct tttacattac agaaaatgag catcagtatc tggttaaaca
     8161 tctgcaaaat tataaaaaat ttttaggaat agaagaagta cagacaactg agatcaaagc
     8221 aaagaaacga tatgtcgatg cgaaaaaagt tcaagaacat catgcaatta ttccgacaaa
     8281 gacgattccg actcctgcac gctttgcgaa gctttccaaa ttacagcaag caatctattt
     8341 gcaagttttg cgcactactg tggctatgtt tgctgcagat tatatctacg aagagacgac
     8401 cgtaatgacg ggcgtacagc aattaaagct aaaaagcatc ggaaagatcc cattacagca
     8461 aggttggcag gaaatcttac gcatcaagtc aaaaaagaaa gaagctcaga ctcttcctcc
     8521 tatcgtagaa aatgaagaag cagaagtaga actgaaaagt atggagaaag aaacacaacc
     8581 tccaaaaccc tataccgaag gaacgttgat cacagcaatg aaaactgcgg gaaaaacgct
     8641 ggataatgat gaagcgcaag aaattctcaa agaagtagaa ggaataggga cagaagctac
     8701 acgagccaat atcattgaaa atcttaagca gaagcattac atcgaagtca ataaaaacga
     8761 aataactgtt acgccaaaag gaatcactct ttgtaaggca gtagccaatg aaccattact
     8821 gacgagcgca gaaatgactg cgcgctggga agggtacttg agaaaaattg gtaaacaaga
     8881 ggggacacca tctgtttttt tagagaatat caaaaaattc atcctacatt tattggcaga
     8941 agttcctaat caaatcgaca aggtagactt ttcagatgag attagcggga cacgtcagat
     9001 aaaagcgatg gagaagaaaa acgatcaatt aggaagatgt ccaaaatgta aaaagggaat
     9061 agtcatgctt tatcctaaaa tcgctacatg cttgaatccg gaatgcgact tcaaattgtg
     9121 gccaacagta gctaaaaaga agctgactaa aacgaatctc agagatttgc tttcaaaagg
     9181 aaagacaagt aaagttgtca aaggttttac tggtaaaaaa gggaaattcg atgcagtttt
     9241 agtattaaaa gaagatatga caatcggttt ttcttttcca tggatcgaaa acagtggaac
     9301 aaaagcagtt gatggaaaca aagaaacatc aagaaaaaat aat
//
LOCUS       DAXWLG010000102         9057 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXWLG010000102 DAXWLG010000000
VERSION     DAXWLG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.103"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(13..837)
                     /locus_tag="ACGE4T_001929"
     CDS             complement(13..837)
                     /locus_tag="ACGE4T_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFF8387175.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(810..1490)
                     /locus_tag="ACGE4T_001930"
     CDS             complement(810..1490)
                     /locus_tag="ACGE4T_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8387176.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1513..2451)
                     /locus_tag="ACGE4T_001931"
     CDS             complement(1513..2451)
                     /locus_tag="ACGE4T_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8387177.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2696..3547)
                     /gene="ispE"
                     /locus_tag="ACGE4T_001932"
     CDS             complement(2696..3547)
                     /gene="ispE"
                     /locus_tag="ACGE4T_001932"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFF8387178.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3701..6274)
                     /gene="mprF"
                     /locus_tag="ACGE4T_001933"
     CDS             complement(3701..6274)
                     /gene="mprF"
                     /locus_tag="ACGE4T_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8387179.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6415..7221
                     /gene="proC"
                     /locus_tag="ACGE4T_001934"
     CDS             6415..7221
                     /gene="proC"
                     /locus_tag="ACGE4T_001934"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFF8387180.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7300..8856)
                     /gene="rny"
                     /locus_tag="ACGE4T_001935"
     CDS             complement(7300..8856)
                     /gene="rny"
                     /locus_tag="ACGE4T_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFF8387181.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2664 a         2010 c         1562 g         2821 t
ORIGIN      
        1 tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt
       61 tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag
      121 ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc
      181 aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat
      241 tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat
      301 cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc
      361 atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac
      421 gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag
      481 ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag
      541 cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt
      601 tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg
      661 attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc
      721 agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc
      781 aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga
      841 gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga
      901 tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga
      961 taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag
     1021 tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat
     1081 tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca
     1141 tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa
     1201 cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga
     1261 atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct
     1321 tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt
     1381 tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca
     1441 ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct
     1501 ccattctaaa acttaattga tactttcttt taaagctttc agattttttt ccatgacaga
     1561 aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac
     1621 ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc
     1681 ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg
     1741 acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta
     1801 ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc
     1861 ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc
     1921 attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt
     1981 tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga
     2041 atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc
     2101 gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga
     2161 atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc
     2221 tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag
     2281 tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt
     2341 cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc
     2401 aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct
     2461 cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg tatcaaaata
     2521 aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta
     2581 aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat aaaaaactga
     2641 agaataccat tttaggattc ctcagttttt catcacctat tttacttctt atattttatt
     2701 gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta tatactctct
     2761 gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca ctcatcaatg
     2821 cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact ggatgccgct
     2881 ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga taatcttgct
     2941 ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc gtatctactt
     3001 ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca acccagcact
     3061 gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat gccgtacttc
     3121 cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc agttcttcta
     3181 acgaacagcc cagattccat aattggttga tccctcttag agcagctgca cagtctgtac
     3241 ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc ttgacaccgg
     3301 ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg ttccgttcat
     3361 ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt atttcttcaa
     3421 aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca tgatacccat
     3481 cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct ttttcaatca
     3541 attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca tagggaaaaa
     3601 taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa cagctttcac
     3661 agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct gtgagcactt
     3721 cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt agctgcatgg
     3781 tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc caatgatcga
     3841 catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta ccataattat
     3901 atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca acattagcta
     3961 atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca ataaacagat
     4021 tggctaacag atagtcagct aattcctcag attctttagg taagacccgc aacaaatcat
     4081 atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact ttttgatctt
     4141 tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt cccacagaga
     4201 agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca gcttgatgta
     4261 attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc aaacgttgat
     4321 attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca atccctgctt
     4381 cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag ctttctgata
     4441 tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac tgattcgttg
     4501 ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat ttgtttcctt
     4561 tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa aaataactgt
     4621 aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc aataaacggt
     4681 caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct aaatattgat
     4741 aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag aaccacacat
     4801 catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa aagcgtccgc
     4861 tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta gcatcaaaga
     4921 ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg gataaccaca
     4981 cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta cgtgtgattg
     5041 tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt ttgactttta
     5101 ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc actgtctgat
     5161 ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt gcagacagat
     5221 tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca cttacgagca
     5281 cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat cgattgattt
     5341 tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga acataataaa
     5401 acaaccgata atataatagc caaactactg ctacatcttg ccgaacagac aattgactca
     5461 ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc gtcagcattc
     5521 ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata tctattctca
     5581 tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca aaggatgcca
     5641 gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt ctctttagat
     5701 aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag atccaatagc
     5761 ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga aacgaccata
     5821 cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa accatttttc
     5881 tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca ccaccaaaac
     5941 cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct tttctttcca
     6001 tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac aacaacatcg
     6061 gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg ctttgttgtc
     6121 ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg acttgatttg
     6181 ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta tgttccttta
     6241 tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa cttgtatact
     6301 tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta gacctgttct
     6361 ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga taacatgaaa
     6421 gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt attaaaaaaa
     6481 ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa tgcacaaaaa
     6541 tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc atcacaatca
     6601 gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga agaactaaga
     6661 gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt aacagattta
     6721 gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa tgtcaatgtc
     6781 agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga ggaacagctt
     6841 cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc agaatcagat
     6901 ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat gctgatcgat
     6961 gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac tgagattgct
     7021 gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa tccatgggaa
     7081 cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt tgccttagaa
     7141 gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga gaaagatctt
     7201 gagatgttga ataaaaaata atgatagcaa tgttttttcg tgccttacaa aaaaagaaga
     7261 gctgtggctt ttgccgcggc tcttcttttg tattttttat tatttagcat aatcagttgc
     7321 acgagtttcc cgaataacga ttactttgat gtgtccagga taatccagtt cttcttcgat
     7381 tcgtttacga atatcacgta ctaaacgaac agcgtctaaa tccgagattt cttctggctt
     7441 gaccattacc cgaacttcgc gtccagcttg aactgcgtaa cttgattcta caccggcgaa
     7501 gctgttcgaa atattttcca gacttcttaa acgacggatg tagttttcta atgattcact
     7561 tcgcgcacca ggtcttgctg ctgagagcgc atctgctgca gctacaagga cagagataac
     7621 agaagtagct tccacatctc catgatgaga agcgatcgcg ttgattacta ctgggttttc
     7681 tttgtacttg gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa tctcatggtc
     7741 aagtgctttc ccaatatcat ggagcaaccc ggcacgtttt gctaattgga tatcttcgcc
     7801 aagctctgct gctaagatac cagctaattt tgcaacctcg accgaatgct tcaagacgtt
     7861 ttgtccataa ctagttctga atcgcagacg tccgatgatc ttgattagat ctggatgaag
     7921 tgtatgagca ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac gttcatccat
     7981 ttctttacga gatttctcga ccatttcttc gatacgcgct ggatggattc gtccatcttg
     8041 tatcagtttt tccaaggtca tccgtgcgat ttctcttcgg attggatcga atccggaaag
     8101 aacaactgct tccggtgtat catcgatgat taaatcgata ccagtcagcg tctcaagcgt
     8161 tcgaatatta cgaccttctc gtccaatgat acgacctttc atttcatcat tcggaagggt
     8221 aacgactgaa acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa tcgctaatga
     8281 taataagttt ttcgctttgc gatctgcttc ttctttggca cgctgttcag attcttttac
     8341 cattagagtt aactcatgat tcagctcttc ttcagtcgat ttcatgatga catctcgtgc
     8401 gtcctctttt gataaaccag caattttttc tagttctttt tgttgatctt caatcatttt
     8461 ttcaacgtct ttttctcgct cttcaattaa ttgctgtttt gaactaagtg cttcttcttt
     8521 actttcaagt gagttctcgc gtttttctaa cgagtcatct ttacgattca gcgtttgttc
     8581 gcgttgaatc aaacgattct cttgagattt taattctaac ttactttctc tcagctcact
     8641 ttcaacttca gaacggtatt tctgattttc ttccttagct tctaacaatg cttctttttt
     8701 tagagtttct gcttcttttt ttgcactatc gatgattcca gcagctgatg attgtgcacc
     8761 agctatttct ttctcgtgac gtgactttgc gaccataaac cctaaaccaa gaccgacaat
     8821 taaaccgatg atagcgagga gaatgttgaa taccataaaa tccacctcca tactatcttt
     8881 ttcattttta tcatctatac agcttctatg ataaattatt ctaaactcgt caatatgcat
     8941 cttcgcatat atgtgtacaa aaaacatgca caactttatt ctaatgtttg taccccgtag
     9001 tgtcaacttt aaataaacaa agaaacggct ttctacttat tcattaaacg atggttt
//
LOCUS       DAXWLG010000103         8945 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXWLG010000103 DAXWLG010000000
VERSION     DAXWLG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.104"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(13..1152)
                     /locus_tag="ACGE4T_001936"
     CDS             complement(13..1152)
                     /locus_tag="ACGE4T_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFF8387182.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACGE4T_001937"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACGE4T_001937"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFF8387183.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACGE4T_001938"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACGE4T_001938"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFF8387184.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACGE4T_001939"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACGE4T_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFF8387185.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACGE4T_001940"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACGE4T_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8387186.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACGE4T_001941"
     CDS             complement(6166..7257)
                     /locus_tag="ACGE4T_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFF8387187.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACGE4T_001942"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACGE4T_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFF8387188.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACGE4T_001943"
     tRNA            8800..8871
                     /locus_tag="ACGE4T_001943"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2321 a         1897 c         1648 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     8941 agaaa
//
LOCUS       DAXWLG010000104         8890 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXWLG010000104 DAXWLG010000000
VERSION     DAXWLG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.105"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(19..1248)
                     /gene="hflX"
                     /locus_tag="ACGE4T_001944"
     CDS             complement(19..1248)
                     /gene="hflX"
                     /locus_tag="ACGE4T_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFF8387189.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1250..2167)
                     /gene="miaA"
                     /locus_tag="ACGE4T_001945"
     CDS             complement(1250..2167)
                     /gene="miaA"
                     /locus_tag="ACGE4T_001945"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFF8387190.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2171..2908)
                     /locus_tag="ACGE4T_001946"
     CDS             complement(2171..2908)
                     /locus_tag="ACGE4T_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8387191.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2999..3526)
                     /locus_tag="ACGE4T_001947"
     CDS             complement(2999..3526)
                     /locus_tag="ACGE4T_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387192.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3706..4299
                     /gene="clpP"
                     /locus_tag="ACGE4T_001948"
     CDS             3706..4299
                     /gene="clpP"
                     /locus_tag="ACGE4T_001948"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFF8387193.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4403..4888)
                     /locus_tag="ACGE4T_001949"
     CDS             complement(4403..4888)
                     /locus_tag="ACGE4T_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFF8387194.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5041..5238)
                     /locus_tag="ACGE4T_001950"
     CDS             complement(5041..5238)
                     /locus_tag="ACGE4T_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387195.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5333..7093)
                     /gene="efrB"
                     /locus_tag="ACGE4T_001951"
     CDS             complement(5333..7093)
                     /gene="efrB"
                     /locus_tag="ACGE4T_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFF8387196.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7090..8820)
                     /gene="efrA"
                     /locus_tag="ACGE4T_001952"
     CDS             complement(7090..8820)
                     /gene="efrA"
                     /locus_tag="ACGE4T_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFF8387197.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2507 a         1976 c         1490 g         2917 t
ORIGIN      
        1 tgttaaaaaa ataaaatttt attggttttt tcttaaccat tttgaattgg tttttgcaaa
       61 ccctttaact tcatattcgt ttgtttcttc catgtacgct tcgtccaaaa ccattgtttg
      121 tcttctcaac tcgcttaatt tctgtccttc gttgcttggc acagaaagtg tataaggggt
      181 cattagttcc atcagctgac gtttgattgc ttcgatcaac gccatcttac cttctttcgt
      241 ctgagtagaa atcaatacat ttgggaatag cgtaggcgtg aacatcgctg ggtcgatctg
      301 gtctgcctta ttgtaaacag tcagtatcgg catttctttc atattcagtt catccatcaa
      361 ttttaagaca gtctgctcct gtaaaatacg atctggagaa ctagcatcga ctacatgtag
      421 caaaagatcc atattttgac tttcttctaa tgtagaatgg aaagcatcga tcagttgcgt
      481 aggcaggtct tggatgaacc cgacggtatc cgttacagta atctcaaatc cttcagcaaa
      541 acgccatctc ttagtcaatg gatctaacgt tgcaaacaat tgatctttcg aataagtatc
      601 cgcttgtgtc aatagattca agatagttga cttcccggca ttcgtgtagc caataagtcc
      661 aatctgaaaa atctcgctat tttttctttt ttggcgattg cgctctcgat gagcttctac
      721 ttctttcaat tctttcttaa cagcaaaaat tttattccgt atatgtcggc ggtctgtttc
      781 taactttgtt tcacctggcc ctcttgttcc gatcccaccg ccaagacgag ataatgtttt
      841 cccttgccct actaaacgag gcagcaggta ttctaactga gcaagttcaa cctgcagctt
      901 cccttctttc gaacgagcac gcatcgcaaa aatatctaag ataagctgca cgcgatcaat
      961 taccggaaga ccaacagcat cggtaatcag ttggctctgt ctcggagtta gctcatgatt
     1021 aaagatgatc aaatccgctt catgcgcatc cgcttgttga actaattctt gtaatttccc
     1081 tttaccgata atcgtttgcc gatccacttg tggtctcttt tgtgtaattg tgaatacgac
     1141 ttctcctgat gcggtattcg tcagctgaac tagctcttcc attgactctg aaaagtaacg
     1201 ctggtttgct tcggtttcta cccctacgat gatcactttt tcttccattt tattccgcct
     1261 ccagccattt tttgattgtt tgctcaatct gacttttttg atttggctca cttataagat
     1321 caaaccactg tgcatccaaa cggttacgaa accacgtcaa ctgacgtttc gcgtatcttc
     1381 tcgaatgtag tttgacttgt tctgttgctt cttccaatgt ttctttccct tcaaaataag
     1441 ggaaaaattc tttatatcca ataccttgac tggcctggac ttctccaagc tcatacacct
     1501 tacgcgcttc tgctactaaa ccttctgcta gcatcaaatc gactctttga ttgattcgtt
     1561 cataaagaat actacgcttc gtatctaatc caatcatgag gtaatcatac aattgctttg
     1621 gctcttcttt aggtgccgtg atactatgtc ctgtcgtctc taaaacttcc agagctctta
     1681 tcactttgcg ttgattgttc cagtggattt tctcagcagc tgccgggtct ttttctttca
     1741 actgattcca taatgcttgg gaaccaattg tttcagctaa cgtttcatac ttttttctaa
     1801 tctcaggatt cgcttcttct gtcgctccta actgataatc atatagaagg gattggatat
     1861 agagacccgt accaccagct atgataggta ggtgccctcg gttcgcaatc tcggtaatca
     1921 attgtcgacc tgaatgctga aaatcagcgg ctgagtaacg ttgagtaggt tcgatcacat
     1981 caatcaaatg atgcggaaca ttattcatct cttcttcttt gatttttgct gtaccgatat
     2041 ccagattttt atagatttgc aatgaatcac cactgatgat ctctcctcca aatagttgtg
     2101 cgagttccac acttagcgct gttttaccga ctgctgtcgg accgacgatc actaatactt
     2161 tttccatatt ttacctactt ttcgttgatt gtttccaccg aagagctttc tccggaaaat
     2221 cagttattat tccatctatt ttgagttctt gacatttggc catttcttct tcatcgttaa
     2281 tcgtccatgc acgaattgct tttggaaaat cagctgcctg gactggatgt tcgaacaacc
     2341 aagtattttt tggatgtact ccttctatat aaggggtttc tgctgctagt tggatctttt
     2401 tatcagaagt actcatcaat aagtccagct gtatctccgg ttccagtaat gcgatttttt
     2461 ccaaagaatc caaatgaaag ctgcaatatg catgttgaaa aggccaatct ctacttttta
     2521 atgtttcggc taatttttct tcgataccgg gatattcata ctgatcagtt ttaatttcaa
     2581 tcattagcat ccctcggtaa tttttttgga ggagcagtcc taacacttct ctcaattcag
     2641 gaactttagc tgctggaaat tctttgctga atttgccccc tgcattcaat tttttgagtt
     2701 cttccaatgt tttttctcga atcagccctt taccgttagt cgtccggtcc acttcttcgt
     2761 cgtgcataac gatcaaataa ccatctttgc ttagatggac atctagttcg ataccatctg
     2821 cacctactcg tacagcttct gcaaatgcag gaagcgtatt ttctgggtgg gtgccgctgc
     2881 ttccgcgatg ggcatagatt tgtaacatct ttcttcactc cgttcagttc tattgtatca
     2941 ttaagaaaaa aagaaagcat taccacgctc tcatgtgcaa cggcaatgct tcaaaatatc
     3001 agctccaaac atcatctttc gagaagaaac caatcggctg gcgcgtcata gtcttcaact
     3061 gagctttttt ttcattgatc atttcagtca gactatccgt aaaattctct aaaatcacgc
     3121 gtccgccttt ataagcatat ccaccaatct tcattgtttc atctggagat aaaatcactt
     3181 ttcgatggga gatattctta aatagctcgt ctaaaatata atttggcaga taaaaactat
     3241 tttgcgctga aaatgattgg agatacgcaa aatcgtcaat cgtgatataa caatcatcaa
     3301 atgcatcaaa gctgacatat tcttccatca gcgttgtccg tttttgcaag atctcgttga
     3361 tctttttatt taattgattc agcgtctcga tttctttgac tttcaattcc aattcacgtc
     3421 tacgctgttc tttattttcc aagtgacgca tttgcagtat catgccgata tagacaacta
     3481 cagcacctgt cagcactccg ataaggccag taataccaac tatcaatctg ttgttcctcc
     3541 ttgttttgtt tctctgctta tcataccata aagcgggttt atgcgactaa tttgcctaag
     3601 ttttttattt gacctttttt gaccaaaaat gtatactaca ttttaggtat tagaaactaa
     3661 attatggtac actatttcta aacgaaatca aggaggaact ctattatgaa tttaattcct
     3721 acagtcattg aacaatcttc acgaggtgaa agagcttacg atatctactc acgtttattg
     3781 aaagaccgta tcatcatgtt aagcggccaa gtaacagatg atcttgctaa ttcgatcatc
     3841 gcccaattgc ttttcttaga cgctcaagat tctgaaaaag atatctatct ttatatcaat
     3901 tctcctggtg gttcagttac agcaggtatg gctatttacg acacaatgaa ctttgtcaaa
     3961 gcagatgtcc aaacaattgt tatgggtatg gctgcttcaa tgggaagctt cttgctgaca
     4021 gctggtacaa aaggtaaacg atttgcttta ccaaatgctg agatcatgat ccatcaacct
     4081 cttggcggcg ctcaagggca agcaactgaa atcgaaattg cggcacgcca tattttgcaa
     4141 acacgcgaac gcttaaacaa aattttagct gaacgcactg gacaaccgct tgaagtgatt
     4201 gaaaaagata cagatcgtga caactatatg acagctgaac aagcaaaagc atatggattg
     4261 atcgatgaag tcatggaaaa cagttcttca ttaaattaat ctgtttcaaa ggaagctttt
     4321 ttaagctctc ttatctaaac caaataaaaa aggatggtca actcaaactg accatccttt
     4381 tttattttat ggatgctact gcctatcttt gctccttgaa ttttccttgc gcatgcatcc
     4441 gacctatctc gacataatgc tggatgtttt ctttgttttt ttgtatttct tcttttgtca
     4501 aaggacggat cactttagct gggcgaccaa aagccaagac attaggagga ataacttttc
     4561 cttctgttac taatgatcca gcaccaatca aactattctc accaataacc gcatgattca
     4621 atatcgtggt ccccatcccg atcagcgccc ctttttcgat tgtacagcca tgtagcatgc
     4681 actgatgtcc taccgtcaca ttctccgcaa tatctacagg tgcatcatga tcgacatgga
     4741 tgattgtacc atcttggata ttcgttcttt gtcctatttt tatccaatta ctgtccccgc
     4801 gtaaaacagc ttgataccag atcgtcacat cttctgacaa tgtcacatca ccaatcactg
     4861 tggcatttga tgctataaac cgttccatat ttatttttct cctttcattt tttgtagaaa
     4921 ctaatcctat tgaataaagg tatcatagca tatatttatt tcataagaaa aaaaacaggt
     4981 aaaacctgct caggccagat gtctatttac agtatctggt aactgacgtt ttacctgttt
     5041 ttattgtaca tctatatttt cttctattac tttttttaaa attaattcgt acacttctat
     5101 ttgtgctgcc aagcgttcga tttgaaactc attttcaaaa aaatcagctg aatcaaaagg
     5161 cttcgtctga cagacgtgcg ttaaacgatc aacttctctt ttggcttcag taattttttc
     5221 atttaaaaac tgttccatct ccgtgtcctc acttttcttg ataactttac tttaccatta
     5281 tcaagaatag agaacaaaaa ataagtttta aactgtttta ttcttatatg acttattctg
     5341 cttcctcagc aaattggctg ttatacagtt tttcataaaa tccaccttgt gccaataaac
     5401 tctcatgcgt tcctttttca atgatctgac cttgatccat cactagaatc agatctgcat
     5461 cacggattgt cgataaacgg tgagcgatga cgaaacttgt ccgtccctcc atcacgcgat
     5521 ccattgcttt ttgaatcaac gcttctaaac gggtgtctac tgaacttgtt gcttcatcta
     5581 aaatcaagat tttaggatcg gagaccactg ctcgggcaat cgtcagcaac tgtttttgtc
     5641 ctaaagacac attgtctccc tcagaattga tggccatatc atatccatct ggcatcgttc
     5701 ggataaaatg atctacgttt gctgttttcg cagcatcaac tacttcataa tctgtcgcat
     5761 caagctttcc gaagcggata ttatctgcaa tcgttccttg ataaagccat gcatcttgca
     5821 agaccatacc aaatagcgaa cgcacatcac tgcgactcat tgatttggtg tcgattccat
     5881 caatccttat tgctcctttg tctacgtcat aaaaacgcat caatagattg atcaatgtcg
     5941 tcttgccggc accggtcgga ccgaccacag ctacggtttg accagctttc acttcaaaat
     6001 tcaaatcttg gattaatggt ttcttcggat catatgcaaa atcgacatga tcgaatgtca
     6061 catctcctaa aatcgtttct ggcagtgaag catctacttt gttttctttt tcttctggct
     6121 catctaaaat ctcgaataca cgtttcgttg ccgctgatgc actttgcaaa actgaagaca
     6181 attgtgtcac gttccccatt ggctgactga tttgccaaat atattggatg aatgcttgta
     6241 gttgaccgac aacgatcgcc ccagtaataa cataaaagct tcctagaaca gccataccaa
     6301 tataagtagc ataggcagtt aattgaacta gaggcatcat caagccagaa atgaatgctg
     6361 cacgaaaccc aaagtatttc aagctgtgat taactttttt gaatccttcc aatgtttctt
     6421 tttcgcgtcc atatactttc aatacactaa aacctgtcat attttcttgg acgtagccgt
     6481 ttagctcacc aagagaattc tgcatgcttt gaaaatattt ctgcgaagca ttgatcactc
     6541 gttttgagat ccagatcgat gttggaatca tgatcataga gatcagcgcc atcatcggct
     6601 ggatataaaa catcattgct gcagccaacg taattcccaa tatcgcattt acgataccaa
     6661 taaaagcttg ttgtaaagca ccgctgactg catctacatc attcgtcaca cgagacaaaa
     6721 tatttccttg ctggttttta tcgaaatatg aaaccggaag acgattgatt ttttcttcta
     6781 tgtctcttct caaatcatgc atggcacttt gaacgacatt tgtcatcaaa acaccagaaa
     6841 gtaattgcga aatacaatac cctattccaa caactgtgat ccaaatgaga catttgaata
     6901 tataagaaaa attgattgat tcgccattgg caatattacg gctgatttct gtagtaggaa
     6961 gtcctgtaac atatggcaaa gccacgttga aagccactgt cagaactgta aataggatca
     7021 ctaaaagaaa agtctcacga tacggtctga tatatcgagc taaacgtttt aaggaagaaa
     7081 aggttttttt catgctaatt cctcctttga taattgagaa gcggcaatat cataataaat
     7141 cgggcatttt tccaacaact cccgatgcgt ccccataccg acgacttctc cttcatttaa
     7201 tacgatgatc ttatctgcat gcatgatcgt tcccacacgt tgtgctacga tcaatacagt
     7261 agattctgtc gtttcttttt tcagacgagc acgtaacttc gcatccgttt gataatctaa
     7321 tgcagaaaag ctgtcatcaa aaatatagat ttccggacgg cgtatgatgg ctctggctat
     7381 tgccaatcgt tgtttttgtc cacctgaaaa attggaacca ccttctgata aatgttcatt
     7441 aaatccatct ggttttcgag aaatgaattc tgaagcttga gcaatctcag ctgcacgttc
     7501 catttcttct tgcgtagcat cttctttccc gtatcgcaaa ttatctgcaa tcgttccagt
     7561 aaatagcagc gctttttgcg gaataaaccc aatcttatct cgcaacttgc ttagccgata
     7621 atctctcacg tctactccat cgatcaagat ctttccttca ctcacatcat aaaagcgagg
     7681 gatcaattgg atcaacgtag attttccgct accggtactt ccaataaaag caacggtctc
     7741 tcctggagag gctgtaaaac tgacatttcg tatcactggc tcttgcgaat ggcctggata
     7801 agcaaatgtt acattttgaa attcgatata ccctttagtt tttgtatcag atataccatt
     7861 tggatcttct acaatcgaag gtttggcatc tagggcttct tggatacgtc cagctgaaac
     7921 agctgctctt ggatacatca tgaacacact agcaaataac atgaatgaga acaaggcatg
     7981 gaatatatac tcgataaacg caatcaaatc tcctacacga agctcgcctg catcgatttg
     8041 cactgccccg ctccagatga tcaataccat tacgatatta aataagaaat aaaaacccgg
     8101 ttgtgcaaca gccatcaaac ggaataagct ttttgaacta gaagcataat tctcattgac
     8161 tacagcaaaa cgtttttctt caaacttttc attgacgaat gcacgaatca cacgcaatcc
     8221 tgaaagattt tcccgcaaga tactgttgat tttatctaag tttttttgtt gctttttaga
     8281 taacggttca gacaccttag caatcgctac gactgccaat aatagtaaag gtaatgcacc
     8341 tactacatac aaactgagtg tcacacttgt acgcatgacc atgtacaaac tgacaatgaa
     8401 catcattggt gccataaaac cgatacgtaa aatattttgc aggaacaaca tgatctgata
     8461 agcatcgttt gttgtacgag tgatcaatga agatacgcct agattttcgt attcctgatg
     8521 cgaataactt tggatctttt cgaacaaatc atctcgaata tctgctacga tattagtcgt
     8581 aatcctcgaa ccaaaataac ctaacaagat attcaaaata ataccgatta cagtaataac
     8641 gatcataaat atcccttgtt gcttcacata atcaaaatca tcattgcgga ttcctacatc
     8701 gatcatacgt gctaaaatcg taggaagtcc taattcaatc aaaataaaac caaatacgca
     8761 tatgaaattg aaaaacaaga atttcttata acgcatggta taatgccaca ttagtttcac
     8821 tttctcatct ccttttaatc ttttgttaca ttttcattac taaaaatgat agcacaacga
     8881 gcctattaat
//
LOCUS       DAXWLG010000105         8778 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXWLG010000105 DAXWLG010000000
VERSION     DAXWLG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.106"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            107..754
                     /locus_tag="ACGE4T_001953"
     CDS             107..754
                     /locus_tag="ACGE4T_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387198.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(847..3126)
                     /gene="helD"
                     /locus_tag="ACGE4T_001954"
     CDS             complement(847..3126)
                     /gene="helD"
                     /locus_tag="ACGE4T_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFF8387199.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKNLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3404..5227
                     /locus_tag="ACGE4T_001955"
     CDS             3404..5227
                     /locus_tag="ACGE4T_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8387200.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5274..5453
                     /locus_tag="ACGE4T_001956"
     CDS             5274..5453
                     /locus_tag="ACGE4T_001956"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFF8387201.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5790..7730
                     /gene="thrS"
                     /locus_tag="ACGE4T_001957"
     CDS             5790..7730
                     /gene="thrS"
                     /locus_tag="ACGE4T_001957"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFF8387202.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7954..>8778
                     /locus_tag="ACGE4T_001958"
     CDS             7954..>8778
                     /locus_tag="ACGE4T_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8387203.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQ"
BASE COUNT         2760 a         1567 c         1874 g         2577 t
ORIGIN      
        1 ttttttgtgt gatttatttt cttttctaat tcaacctgtg gtatgatgag agcataagta
       61 tcatgtgaaa tgatatgaag tatgtggaag atggacgggg aagaatatgg aagcaaaaat
      121 cactttagaa ccttttgaac gtatcttaag cggttaccgc aaagtagaag agttagctgt
      181 gaacgtaact gattgttcaa aattagcgca aaaatacgca agattcggcg ttgaaggcta
      241 tcgattagga aactatgtcg gtacagggta tctgaaccgt tatttagagt gtatggtgga
      301 ccgcgcacca atgctgattt atagacaaaa ttatctgatt ccgctgcttt ttcgtcgttc
      361 tgattcagcc tttcggttat ttgaagaaga gtatcggatg gaggctttct ttctactatt
      421 agagtggagc ttgaaacatc gtcctgaaaa aattcttatc gaaagaaatg agaagataga
      481 cacgaaaaaa aataaagtga tcgatagtgc atacctagca tttcgtgtat cagagatttt
      541 ggattgtggc ggctatccaa tcagcaattt ccaatcaatt gaccaattca ttgaatggaa
      601 tcgaatttac cgattgatcg acaatggcgg gattggccgt catagcaagg tatttgaccc
      661 agaatatcca gaaaacatgg aagaactgaa aatgattatt tcgcttgtca aattaaaata
      721 tcctgaaaca gatttggatt tatatataga ataaatgacc ttaaatgatt gaattacttt
      781 ttctgcaatt attattgtaa ataaaaagac aactgctaac attaatgata gcagttgtct
      841 ttttacttac agtacttttt tctcataaga tgaaatatcc atattgttta acaaaggact
      901 ttcattattg gaggtaaaaa gggtcaattc atgcatcgca cgtgtacaca tggtgtaaag
      961 gatcaatttg tcttgctgag aagaaaaatg ttctgcatct acatcccaca agaaaactcg
     1021 gtcaaactcc agaccttttg ctaaaaaggc tggaagtaaa ataatggaac gcttcataaa
     1081 gtcactctca tttaaaatca gctggacttg ttgtttcaac tcgtctggca gttgagcata
     1141 aagagcttcg cattcttctt gtgttttagc tatgattgct gtacgccaat accgggcttt
     1201 tccctgagtc gttcgaatgg tatctgctaa ccaattcaat ttgatatcat ctgttgttcc
     1261 ttggatgagc aaaggaaggt ctccttttct tgctgttgtt tctacttgat gttctttcac
     1321 aataaaatga ttagcaaaat cagtgatttc ttttgtggag cgatagcttg tcgttaattg
     1381 gaaacgagtg acttcacggt ccacgaaaag gtcttccaat gatccaacga tcgtttcatt
     1441 tccaaagaca ttttgattta gatctccaca gaaagtcaaa tttgcttttg ggaacaaggc
     1501 ttgcagcaat gccacttgtg atggtgcgaa atcctgcatt tcatcaataa agatatagcg
     1561 agctttgtgt gtaaccgtca ctggatatgt tcggcgcatc agtaaaaaga atagtacagc
     1621 atcctcttga cgcaattctc tttgacgcaa gtgtgtacga acagttgtca aatgttcctg
     1681 ccacctggtc tcgctgattt tatgtcgtgt tagaagagaa agtggcacag tttgtagaaa
     1741 gtggagatat tgtttcgtta tattgacaaa ttgataattc atgatccctc gatggattcg
     1801 acggaatctt ttacgtacga tttggttggc caggttttta cgtaatgaac gctccttttg
     1861 ttctgtatat tcaagatttg gatctgttgc gtatagttct tggagctgtt cttccgccag
     1921 atctttgacc cattgctgac gtgtttcgtc tttttgcaga ccgcctaatt ttttcaggag
     1981 cttcgtttgt aatagagaaa gtctatgatg taaaggtaat tgttcatttg tttctttata
     2041 ccattggcga atcgattcct ttgataggat cgtacggcca tttattttca tatcacggaa
     2101 caagggaccg tagtttgtga tgccttggat atagcgatca atctgatcga ctaaggtcaa
     2161 tccgcttttc atcacttgta tcggatcttt ttcccctagc aaaaaaccac tttcttgttg
     2221 ctgttcatct agtaaagaaa actctggcag cagctgcacc aaataatttt tgaatgtctg
     2281 tgtaggcacg ccgctttcac ctaatgaagg caatacagtc gagatgtaat cagaaaacaa
     2341 atggttgggt gaaaatagaa gaacgttttc agcctcgagc cacttgcgat tatgatacaa
     2401 cagaaaagca atacgttgaa gcaaagcgga tgtctttcca cttccggcga ttccttcaat
     2461 cagcattact ttacttgttg tgtcccgaat gattgcattt tgtgcttttt gtatcgtaga
     2521 gacgatgttt ttcatatggt tgcttgatga ttcatccaat atctctagca aaaagtcatc
     2581 attgattact tccgacgtat ctaccataga taaaatacgt ccttcttgga ttttaaattg
     2641 tcgtttcaac tgcagatcga catctattct atctgtttca gtttcataaa atgcgggacc
     2701 aatctcacct tcataataaa gattggcaat tggtgcgcgc cagtcaataa cgattgtttc
     2761 ttctgccaga tctcttaaag aagcgatacc tagatataat gtttctgttt cacggtcttc
     2821 tttaaagtcg atacgggcaa aataaggact atctttcatg acagacaacg tttgcaggcg
     2881 tttttgctgt gcttcaagag tatggtattt caaaagcaat tcctgctcat gttggcgata
     2941 ttcaacagca gattcataaa aagcttcatt ggaaccagct cgaatcttgt gattagctgt
     3001 gtcttttgtc gaatgattca tttcctgagt caattcttgt tgttttgtat ccagcatatt
     3061 ttgttcgatc tggattagtt cgatcgtttc atccacgtgc gtttgttcta gttgtttctc
     3121 attcatcgca tgtttgctcc ttccgccgta aataacgaat taaaagtata gcatagtttt
     3181 tcgaaaaaag gtacttttta actgatgaat cgaaaaagta atccttgctg aatatttttg
     3241 tttgtagaac tgatgaattt tggaaacgct gatagtgata gttgttttca attaaggcct
     3301 attgttttaa aaagtgctag tcaaggacta tgcttaagat gaaagaggca gctggaaatt
     3361 tattactaac ctctctaagt cacataaaag aggagaaaaa acaatgacac atttcaaaaa
     3421 atttttactt actttgctta ttggtgggat agctttatta agtgaatttg tttttcacca
     3481 gccggccctt gcatttgcga ttgtcgcatt tactggaggg attctcgcct tcttgatgtt
     3541 tttggaaatg atcaagacgt tacgttcagg aagatatggc gtagatgtat tagcgattac
     3601 cgcaatcctt gcaacattag ccgtcaaaga atactgggct agtttgatga tattgatcat
     3661 gcttacgggc ggagatagtc tagaggatta cgcgaataaa cgagcgggta aagaactgca
     3721 gtctttgtta gataacaccc caagaattgc ccatcgattc aaagaaggaa aattgcagga
     3781 tatagtaatc gatgaaataa atattgatga ccagttacta gtcaagcctg gcgaacttgt
     3841 acccgcagat ggtcgagtga ccaaaggaga atcttcggtc gatgaatctt ctttgacagg
     3901 tgagtcccgc ccggaaacca agaaacctgg agatcagatt atgtcagggt ctttaaatgg
     3961 agacggatcg atccaaatga tcgctgaaaa acgtgccgca gacagccagt atcaaatgat
     4021 tattaaacta gtcgaagaat caaaagaaaa gcctgcccga tttgtccgtt tagctgatcg
     4081 ttatgcagtc cctttcacgt tgattgctta tttgatcggt ggtatttctt ggtggttctc
     4141 aaaagaccct ttacgatttg ctgaggtact ggtagtggca tcaccttgtc cactgattct
     4201 agctgcgccc attgctttag ttgcaggaat gagccgttca agtaaaaacg ggatcgttgt
     4261 aaaaactgga acaacgattg aaaagctcgc acgtacaaaa acgattgctt ttgataaaac
     4321 aggaacgctt accaaaggga tattggaagt agaggcgatt cagccagtca atggaataaa
     4381 aaaagaagag ctacttctgt atgcagctag tgcagaacag gaatctagcc atatcttagc
     4441 tcgttcgtta gtgaacaacg tgaaaaacag ccaactgtat cctgttagtc aactgaaaga
     4501 agtgaccggt caaggaatcc aagcagtagt caataatcat gaagtaaaga ttggacgtgc
     4561 ctcttttgtt tcagctgaag aaatagaaac gtccaataca gcaatctatg taacaattga
     4621 tcgatctttt gcaggaacga tcctattttc ggatcagatt cgcccagaag cagcaaagac
     4681 aatcaaggag ctaagaaaac ttgatgtgaa aaatttgatg atgatcaccg gggatggatc
     4741 tgccattgct caaacgattg cagaaaaagt ccaattagat tctgtacatg cccgtagctt
     4801 gcctcaagat aaactgacca ttttagaaag tatccctaaa gatcaaagac ctgttgcgat
     4861 ggtaggagac ggcgtgaatg acgcaccagc acttgctgct gcagatatcg gtattgcaat
     4921 gggggctcat ggttctacag ctgctagtga aagtgcagat gcggtcatct tgaaagatga
     4981 tctcttgcgt gtagcaaaag cagtagtgat ttcaagagat acgatgagaa tagcgaaaca
     5041 gtctgtactg attggcatct ttatttgtat ttttttgatg ttaattgcaa gtacgggact
     5101 aatccccgca ttattcggag ctgttttgca agaagtcgtg gatacggtct ctattttgag
     5161 tgccttgaga gcaaaaaaag atccagccat ttttcctgat tcagtaccca agacaaacat
     5221 agtatgatag aatcgagttg aaaaaagaac gatcagaaag aggagagagc attatgccat
     5281 tcgtacatgt cgaattagta gaaggtcgca gtccggaaca attagaaaat atgatgaaag
     5341 atatcacgga agctgttcat aaaaacacgc aggcaccaaa agaacacatc catgtcatca
     5401 tcaatgagat gaaaaaagga acatacggtg taaatgggga atggaaaaaa taagtttagt
     5461 ttcccacttg atttttctat ttattagtgg ttaaataaac acataattga ttgatgagaa
     5521 agccaactga atttgagagt cttcgataag agaggaaaag ctaggctgaa acttttccag
     5581 gagaaaacca aaggatgacc actttcgaaa cccttgttga agcaagggcg gtcggaccgt
     5641 tacaacgctt gaggaacgca tttgcgtttg aacattaggt ggaaccacga acttttcgtc
     5701 ctagtatctc ttttcaagag atgctaggat ttttttgttt ttcagcttga tgatctgatc
     5761 aatcaaaaca tttcaaggag gaaactatta tgataaaaat tacttttcca gatggtgcag
     5821 tcaaagaatt tgagtctgga acaacaacat tggcaattgc tgaaagcatt tcaaaaagct
     5881 tagcaaaaaa agctttagct ggtaaagtga acggaaaact cgttgatttg actcgtccaa
     5941 tcgaggaaga tgcaagtatt gagatcatca caccagacca tgaagatgca cttggcttag
     6001 tccgtcattc agcagctcat ttgatggccc aagccatgcg tcgcctctat ccaaatatcc
     6061 actttggcgt tggaccagct atcgattcag ggttttacta cgatacagat aatggacaaa
     6121 accaagtaac tgcagaagat ttgccggcaa tcgaagcaga aatgatgaaa atcgtcaaag
     6181 aaaatctgcc gatcgaacgc cgtgttcttt caaaacagga agcattagaa atttttgcaa
     6241 gtgatccgta caaagtagaa ctgattagtg aattgccaga agaagaagtg attactgctt
     6301 atcaacaagg cgaattcatc gatttgtgtc gtggaccaca tgttccatcc actggccgta
     6361 tacaagtatt taaattatta tcagtggcag gtgcttattg gcgtggaaac tcaaacaatc
     6421 aaatgatgca acgtgtatac gggacagcct tttttgacaa gaaagctttg aaagaatata
     6481 tccgtctccg tgaagaagca aaagaaagag accatcgtaa actaggaaaa gaacttgact
     6541 tgttcatggt ttccccagaa gtaggttctg gtttgccatt ctggcttcca aaaggggcaa
     6601 caatccgacg cacgatcgaa cgttatatcg tagacaaaga agttagtcta ggttaccaac
     6661 atgtatatac accaataatg ggagatgttg agttgtacaa aacatctgga cactgggatc
     6721 attatcaaga agacatgttc ccgccaatgg atatgggaga tggcgaaatg cttgttcttc
     6781 gcccaatgaa ctgccctcat cacatgatgg tctacaaaaa cacgatccat tcttatcgtg
     6841 aattaccaat tcgtatcgct gaattaggaa tgatgcatcg ctatgaaaaa tcaggtgctt
     6901 tgtcaggttt acaacgtgta cgagaaatga cgttgaatga tggtcataca tttgttcgtc
     6961 ctgatcaaat caaagatgaa tttaaacgta cgcttgaatt gatggtggca gtatatgctg
     7021 acttcaatat tacagattat cgtttccgct tgagctatcg cgatccaaac aatacagata
     7081 aatattttga tgatgacgca atgtgggaaa aagcccaagc aatgctgaaa gccgctatgg
     7141 atgaattaga actagattac tttgaagcag aaggcgaggc agccttttac ggaccaaaac
     7201 tagatgttca agtcaaaaca gctcttggta cagaagaaac attatcaact atacaattag
     7261 acttcttatt gccagagcgc tttgatttga catatgttgg agaagacggg gaaaatacgc
     7321 atcgtccagt agttatccac cgcggaattg tttctacaat ggaacgtttt gtcgcttatt
     7381 taacagaagt atacaaaggc gcttttccaa catggttggc acctatacaa gcaacaatca
     7441 tccctgtttc tgttgatgca catggcgatt atgcttatga aatcaaagaa cgtttgcaaa
     7501 tgaaggggtt gcgtgtagaa gttgatgatc gtaatgaaaa aatgggctat aagatacgtg
     7561 cctctcaaac acaaaagatc ccttaccaat tagttgttgg agacaaggaa ttggaagatg
     7621 caacagtgaa cgtacgtcgt tatggaagta aagaaacagc tgtagaagat ttgaatatct
     7681 ttattgatgc tatggaagca gaagtcaaaa attacagcag agaaaactaa taggtaaatt
     7741 tcctgtaaaa aagtagtaga tagaaagcaa tccgaacaat agataacagg tattcctcaa
     7801 agacacagat gctcaataga atctgtggga ttgatgggat aagatattct gttgggcgga
     7861 ttgtttttat gttttttatc cagtatacga ccgtgaataa gggtctgata tggtatactt
     7921 acatagacta tcattaaaag ggtggaggaa cttatgagcg taattagtcg tattgaaagt
     7981 atgatggaag attattcttc agcagaaaaa aagttggcca actacattat cggtaatatt
     8041 gagaaggtcc ctacaatgac agctaatgaa ttagcagaag cttccgggtt gagtgcgccg
     8101 acagttgtac gtttttccaa aaaaatcggt tttcaaagtc tgacggactt caaaatcacg
     8161 atttcaacag aactacagac tggaatcgat gaaggtttta gtgatataga agcaaatgag
     8221 tccttttact ctatcaaaaa caaacttggt aacaatgcac aagtagcgat aaaagaaaca
     8281 gtagatattc tagaagaaga aacgatccaa aaagtcgtag agtgtttgga atcggcagag
     8341 actgtttttt tatatggggt tggcgcctct tctttagtag tagaagatat tctgcaaaaa
     8401 tggtctcgtg tcggtaagcc aatcattttt gaaaaagata tccacgtctt gctcccgcaa
     8461 cttgtcagca atgagaaaaa gaaagtgctt tggcttgttt caaactcagg tagaagtgca
     8521 gatgtagtcg cattagccga gctggcgaaa agcatgaata tcgaaatcat tgcgttgacg
     8581 caatttggaa ataacccgct tagcaagata gccgatgtac tcgtacaaac atctagacca
     8641 aaagaaatca cgaatcgaag tgcggcaact aattcacttt tggcccaatt tgctacgatc
     8701 gatatcattt tttatttcta tatggctaaa aacgaaaagc ttagcgaaaa agtgaaaaaa
     8761 acacgagaag caatccaa
//
LOCUS       DAXWLG010000106         8603 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXWLG010000106 DAXWLG010000000
VERSION     DAXWLG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.107"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACGE4T_001959"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACGE4T_001959"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFF8387204.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="ACGE4T_001960"
     CDS             complement(1098..2201)
                     /locus_tag="ACGE4T_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFF8387205.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACGE4T_001961"
     CDS             complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACGE4T_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFF8387206.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="ACGE4T_001962"
     CDS             3528..5462
                     /locus_tag="ACGE4T_001962"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8387207.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="ACGE4T_001963"
     CDS             5919..6335
                     /locus_tag="ACGE4T_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFF8387208.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="ACGE4T_001964"
     CDS             6349..6915
                     /locus_tag="ACGE4T_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFF8387209.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="ACGE4T_001965"
     CDS             6938..7924
                     /locus_tag="ACGE4T_001965"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8387210.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="ACGE4T_001966"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="ACGE4T_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8387211.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1658 c         1750 g         2668 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DAXWLG010000107         8559 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXWLG010000107 DAXWLG010000000
VERSION     DAXWLG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.108"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(105..560)
                     /locus_tag="ACGE4T_001967"
     CDS             complement(105..560)
                     /locus_tag="ACGE4T_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387212.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="ACGE4T_001968"
     CDS             complement(714..1922)
                     /locus_tag="ACGE4T_001968"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFF8387213.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="ACGE4T_001969"
     CDS             complement(2034..2366)
                     /locus_tag="ACGE4T_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8387214.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="ACGE4T_001970"
     CDS             2514..3389
                     /locus_tag="ACGE4T_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8387215.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="ACGE4T_001971"
     CDS             complement(3471..4052)
                     /locus_tag="ACGE4T_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFF8387216.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="ACGE4T_001972"
     CDS             complement(4058..5317)
                     /locus_tag="ACGE4T_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8387217.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="ACGE4T_001973"
     CDS             complement(5319..6365)
                     /locus_tag="ACGE4T_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387218.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="ACGE4T_001974"
     CDS             complement(6600..6875)
                     /locus_tag="ACGE4T_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFF8387219.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /gene="der"
                     /locus_tag="ACGE4T_001975"
     CDS             complement(7118..8428)
                     /gene="der"
                     /locus_tag="ACGE4T_001975"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFF8387220.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT         2403 a         1793 c         1450 g         2913 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaa
//
LOCUS       DAXWLG010000108         8521 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXWLG010000108 DAXWLG010000000
VERSION     DAXWLG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.109"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            54..341
                     /locus_tag="ACGE4T_001976"
     CDS             54..341
                     /locus_tag="ACGE4T_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387221.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            757..2295
                     /locus_tag="ACGE4T_001977"
     CDS             757..2295
                     /locus_tag="ACGE4T_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFF8387222.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2292..3257
                     /locus_tag="ACGE4T_001978"
     CDS             2292..3257
                     /locus_tag="ACGE4T_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387223.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3244..4566
                     /locus_tag="ACGE4T_001979"
     CDS             3244..4566
                     /locus_tag="ACGE4T_001979"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8387224.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4629..4733
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4807..5550
                     /locus_tag="ACGE4T_001980"
     CDS             4807..5550
                     /locus_tag="ACGE4T_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8387225.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5565..6557
                     /locus_tag="ACGE4T_001981"
     CDS             5565..6557
                     /locus_tag="ACGE4T_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8387226.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6569..7306
                     /locus_tag="ACGE4T_001982"
     CDS             6569..7306
                     /locus_tag="ACGE4T_001982"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387227.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7296..8018
                     /locus_tag="ACGE4T_001983"
     CDS             7296..8018
                     /locus_tag="ACGE4T_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8387228.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8102..>8521)
                     /locus_tag="ACGE4T_001984"
     CDS             complement(8102..>8521)
                     /locus_tag="ACGE4T_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFF8387229.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2752 a         1463 c         1835 g         2471 t
ORIGIN      
        1 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
       61 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      121 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
      181 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
      241 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
      301 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
      361 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
      421 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
      481 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
      541 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
      601 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
      661 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
      721 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
      781 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
      841 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
      901 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
      961 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1021 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1081 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1141 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     1201 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     1261 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     1321 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     1381 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     1441 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     1501 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     1561 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     1621 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     1681 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     1741 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     1801 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     1861 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     1921 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     1981 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2041 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2101 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     2161 aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca agtcaatgtc
     2221 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     2281 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     2341 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     2401 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     2461 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     2521 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     2581 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     2641 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     2701 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     2761 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     2821 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     2881 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     2941 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3001 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3061 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3121 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     3181 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     3241 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     3301 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     3361 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     3421 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     3481 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     3541 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     3601 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     3661 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     3721 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     3781 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     3841 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     3901 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     3961 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4021 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4081 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4141 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     4201 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     4261 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     4321 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     4381 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     4441 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     4501 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     4561 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     4621 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     4681 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     4741 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     4801 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     4861 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     4921 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     4981 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5041 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5101 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     5161 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     5221 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     5281 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     5341 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     5401 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     5461 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     5521 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     5581 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     5641 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     5701 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     5761 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     5821 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     5881 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     5941 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6001 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6061 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6121 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     6181 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     6241 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     6301 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     6361 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     6421 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     6481 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     6541 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     6601 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     6661 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     6721 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     6781 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     6841 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     6901 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     6961 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7021 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7081 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7141 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     7201 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     7261 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     7321 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     7381 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     7441 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     7501 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     7561 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     7621 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     7681 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     7741 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     7801 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     7861 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     7921 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     7981 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8041 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8101 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     8161 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     8221 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     8281 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     8341 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     8401 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     8461 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     8521 a
//
LOCUS       DAXWLG010000109         8515 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXWLG010000109 DAXWLG010000000
VERSION     DAXWLG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.110"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            476..1339
                     /locus_tag="ACGE4T_001985"
     CDS             476..1339
                     /locus_tag="ACGE4T_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8387230.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACGE4T_001986"
     CDS             complement(1468..3090)
                     /locus_tag="ACGE4T_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8387231.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACGE4T_001987"
     CDS             3339..3860
                     /locus_tag="ACGE4T_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8387232.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACGE4T_001988"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACGE4T_001988"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFF8387233.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACGE4T_001989"
     CDS             5941..6759
                     /locus_tag="ACGE4T_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFF8387234.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACGE4T_001990"
     CDS             complement(6862..7059)
                     /locus_tag="ACGE4T_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387235.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACGE4T_001991"
     CDS             7228..7806
                     /locus_tag="ACGE4T_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8387236.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACGE4T_001992"
     CDS             7901..8038
                     /locus_tag="ACGE4T_001992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387237.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2580 a         1521 c         1671 g         2743 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaa
//
LOCUS       DAXWLG010000110         8508 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXWLG010000110 DAXWLG010000000
VERSION     DAXWLG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.111"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(109..1077)
                     /locus_tag="ACGE4T_001993"
     CDS             complement(109..1077)
                     /locus_tag="ACGE4T_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFF8387238.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1309..2142
                     /locus_tag="ACGE4T_001994"
     CDS             1309..2142
                     /locus_tag="ACGE4T_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFF8387239.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2176..3015
                     /locus_tag="ACGE4T_001995"
     CDS             2176..3015
                     /locus_tag="ACGE4T_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8387240.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3041..3601
                     /locus_tag="ACGE4T_001996"
     CDS             3041..3601
                     /locus_tag="ACGE4T_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8387241.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3800..5521
                     /locus_tag="ACGE4T_001997"
     CDS             3800..5521
                     /locus_tag="ACGE4T_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFF8387242.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5686..6828
                     /locus_tag="ACGE4T_001998"
     CDS             5686..6828
                     /locus_tag="ACGE4T_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8387243.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6844..8055
                     /gene="thiI"
                     /locus_tag="ACGE4T_001999"
     CDS             6844..8055
                     /gene="thiI"
                     /locus_tag="ACGE4T_001999"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFF8387244.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2919 a         1474 c         1760 g         2355 t
ORIGIN      
        1 atttacgaaa attgtttctc aaatttttgt aaattttcga cttagttttc gaagaagcta
       61 tttttgtcac agcctctctt tttaagttta gctattagat aatgatgttt agttttggat
      121 aaacgaaatc aattcttcac ccgacaaacc atagtttaca gcaatcaata gttttaaacg
      181 tgctttttga ctattaatac tgttacagaa aataatcccc attttttcta gttcttttcc
      241 cccgccttca taatcataaa caggctctgc gattccattg aaacaacgag aaacaagtac
      301 tacagggatt ttttggacaa gaagcttttc caaagcagct aaggcttgtg gagggagatt
      361 ccccgcacct aaagcttcga ttaccaaacc gtctactttc gtatgggcga tggcttccaa
      421 taagtcccct tgcatcccag catatgcttt aacgatcggg atagtcccat ctacagcgga
      481 aatgtccagt cgctttgttt ctaaaagttc ctgcaagaat aaaattcgat tcttagttac
      541 taagccgatg ggacccaatg taggtgttcg gaaagtagca acatttgtgg tatgggtctt
      601 tgtcacgtag cgtgcagaat ggatctcatc gttcatgacc accaaaaccc ccttatctag
      661 cgcttcttca caacttgcga cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc
      721 aagttcatta cttgaacgca ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt
      781 tgtatcaaga aaaaaagcag tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc
      841 ggatatacct gatgcataag cggacaagat acgttctttt aataccaaca tgtgagaagg
      901 catgatgtgt ggtgagggaa gattgaagat atcttcaact aataattcta catgcttggg
      961 gattgtgatt tctgcatcta ataatgggtt tgccacatca ggactaacac cgcccgtgag
     1021 catgttttct ttcatggcga tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt
     1081 acagacttcc ttacgaataa ttttgcatag ccgttaaatc ttattgtata ctatataaaa
     1141 gagttttgct atcagtgaag aataacgaat agcctaactt atcataaaaa agctgacctt
     1201 attaaaatgg aaaagactca accttgacct cctaatacaa tggagtatct ggttgagttt
     1261 cttttacaag aaagcagcca aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc
     1321 ggattaacat cgtttaaaga acatgacaaa ttaactggta aaaagaataa cactctttat
     1381 gaatatgcag ggtttctacc cttagtggaa ttagatactg cctattatgg aattccccga
     1441 aagtcctcag ttgagaattg gataagtgaa gtacctgaat catttcgctt tgtagtcaaa
     1501 gtctatcaag gaatcagcgg tcaaggagac tggaagcctt attatcagac cgaagaagag
     1561 atgattgcgc attttttaga tgttatgtcg ccaatggtag atgctggaaa gatgttttgc
     1621 tttttagtcc aattcccctc atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc
     1681 aaacttagaa agtggtttga gggttatcct gtttctatag aattgcggga ttattcttgg
     1741 tacagcgatg aatttcttga acgtacaaga aactttatgt ctagagccgc tttttcttta
     1801 gccatcatcg atgagccaca gttgctgaat acaacgattc cttttgatcc atttgtcacc
     1861 aatccgaact ttgctctttt tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat
     1921 gataaagagt ggcgtaaaaa aagaacgtta tatcgttatt cagaaaaaga actgcaagaa
     1981 ctagcacatg aagtcaaagt agttgattct aagacaaaag aggtaggtgt gatttttaat
     2041 aataattctg gtggtgatgc ggctgaaaat gcattagcac tgcaaaaaat attaaatatc
     2101 aagccagatg gactcaatcc aacccaaatg gatctgtttt aagggcttat tttattcaag
     2161 aaaggggcga agtctatgat caaagctatt ttttttgata ttgacggaac attggtcacc
     2221 aatcgttcaa aagcgttgga atcaacaaaa caagcaattg aatatgctcg gaaaaacggc
     2281 attttatgcg gtgtagcaac ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa
     2341 ttggaattag atatgtatgt cgtgtataat ggtcaactgg tttttactgc agatcgcacg
     2401 attatcgacc acccttttga acaaaaagta ttggaacata ttgttgaatt tgcagatgag
     2461 aaccatcgac aaatcgtttt tggtgcgaga aatcggttag atggatcaac gacgatgcta
     2521 ttaggacaat cgatttttat caagcgacta gtcagctttt taccgcgtaa attcccggta
     2581 cgtctgatga aaaaaatcct ccaggttttt agccctcacc gacaaaagga tcgttatgaa
     2641 acgttagaga tattaaaaga accaatttat caatgtatat tgctcagtcc ggagtcagaa
     2701 cagaaaaagt tagaacatcg ctttcccgag tgtacgtttc agcgttcaaa tacgtatact
     2761 gtagatatca tcccaaaagg tggttcaaaa ttactaggca tccaagcttt cgcaaatgct
     2821 gtcgaaattg aaatggaaga aattatggca ttcggcgatc attacaatga tatcgagatg
     2881 ctgaaaggtg taggaattgg agtggccatg gggaatgctc agatcgaagt aaaacatgct
     2941 gcagactttg tgactcaatc aaatgaagaa gatgggatat acgaaggact gaaacacttc
     3001 ggtctgattt attagtcaat gaaagaagga aaaaatcagt atggaagcag agacaccttt
     3061 tcaaaaagta gaaacattcc acaaaacttt tgatccaaga agaccagaga tacccacggc
     3121 ttttacagaa aatgaagctc atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt
     3181 tgttgcagct acttcaccta ataaagaaca actgcatgaa gctgttatgc tgctgcacga
     3241 agcattagat aaagcagaag aaaaaatact tggtaaaaca cagtggggaa gtgatccgct
     3301 ggttgaacaa gtagatgcac tgacagatat cctttacttt acttatggtt ctttttcatt
     3361 actaggaata gtgcctgatc gaatattcga aatcgtccat caggcaaata tggggaaatt
     3421 atttccagat ggaaaacctc attatgaccc tgtaacaaat aaagtgttga aacctgaaaa
     3481 ctggcagatg gaatatgctc cagaacctaa aatcgcggca gaaatccaac gtcagattca
     3541 agaagcgatt gctaagaaga aatccgctga tataggcatg tgcacaaatg aaaaagagtg
     3601 atcagagttt cactattttt gttatcttta tacgatggaa aaacaaaaga ttttcgttta
     3661 agttagtgaa tgcatgaaaa tagtatgcta taatctagaa tagatgtctt ttgcacgaga
     3721 gacgtattat ttggatttat gccaagcaga acgccatctg ttgcataaaa gatgcatact
     3781 gaaaacggag gaaccataaa tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat
     3841 tatagtcgcc attgtcgtct ttttggttag ttacttgatg aagaaaaaaa accaagatcg
     3901 attggatgcc ttagaaaaac gcaaggaaga attatttgac ttgccagtga ttgaagaagt
     3961 cgatgatgtt aaaaaaatgc atctggttgg gcaaagccaa aatacctttc gtgaatggag
     4021 tcaaaaatgg aatgacatat ctacttcctc gttcgctgaa ctagaaagtc aaattttcga
     4081 agtggaaaac ctcaatgaaa gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc
     4141 agaagctaca atggaccaaa tggagaaaca agtgacggaa atccgtgaag gtctaaagga
     4201 attgcgggaa acggaagaac gcaactcact agctgttcag caggcacttg acgtctatga
     4261 agaactgaaa gaaaaagtca gagaagacga gcatgaatac ggaccagctc tttttgaatt
     4321 gcaaaaacag atcaaagtga tcgaaaaaga atttacgcag tttgtgactt taaacacttc
     4381 tggagaccct gttgaagccc gcgaggtttt ggataaagca gaaaaacata cgtatgaagt
     4441 ggaagctctg atgaaaaaaa ttccaccgct gtatgaggag ctccatacca ctttccctga
     4501 acaacttgaa gaaatcagtg atacttacga gaaattgatg gaagaacaat atgtattccc
     4561 agaagaagat cttgctgaag atatcgcaaa agtaagtcga agaattgaaa attcattagc
     4621 caatcttgaa aaaacagaag ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt
     4681 gattgattcg ttatatgata tcttagaacg ggaaatggaa gctcagagat atgtaaaaac
     4741 taaccaatca acgattgcag agtatatcaa acatacaaca aagaataacc gtcaattatt
     4801 gattgagctg gatcacacat ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt
     4861 acgaggtttc caaaccgaag tggaagaaat ggaacgtcaa aatgaacaga tgattccaca
     4921 gatccgtcaa cacgaaattc cttattcaga agtgcgcacg ttctataaaa cagtcttcaa
     4981 agtcttagaa gatatcgaaa agcaacaagt agagatcgac gactcattac atgaattgag
     5041 aaaaggcgaa aaagaagcgc aagaaaagat cgatacattc gaattcaaat tgcgcagttt
     5101 gaaacgtttt gtagaaaaac aacgtttgcc tggcttgccg aatgattacc tagaattctt
     5161 ttttgttgct actgaccgta ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa
     5221 catggaagag gtcaatcgtt tagttgctct atgtgaagaa gatctagaat tattagataa
     5281 gaagacacat gatttagtag atgcagcagc attaacggag caaatgctcc aatatgcaaa
     5341 tcgttatcgt catacacacg aagaagtgag agaagcaatt gataaaagct actatttatt
     5401 caataaagaa tatcactatc aagaagcatt agacgaaatc ggtacagcat tagagcgagt
     5461 agaacctggt gctttcaaac gcatcgaaga tttttatttt aatcatccag acttagtata
     5521 aagcaaaaaa gttagtaaac cagcaagagc aagacggatt attgtcttgt tcttgtcttt
     5581 ttttgtatat ttgaaaattg aaacaagtga aaatttcacc tgctcttgaa agcaccttgg
     5641 acaaccgtta taatagaaaa gaatttcaaa aaagaggggt ttcttatgat atattttgat
     5701 aacagtgcaa caaccccaat ctttccgcaa agcttagatg catatgtaaa aaccagtcag
     5761 cggattattg ggaatccatc aagcttgcat gatttaggca atcaagctaa ccggttgttg
     5821 cagcaagcaa gaaagcagat cgctgatttg atccatgtag aaccagaaga aatctatttt
     5881 acttcaggcg gaacagaagg agataactgg gtattaaaag gaacgatgat cgaaaaaaga
     5941 gaatatggga atcatatgat catttcagga gtcgaacatc cggccgtcag cgagacagca
     6001 gaacaattaa aagaattagg aatcgaagta agtatcgccc cagtggataa acgcggattt
     6061 gtacgtgtcg acgaattgaa agaattaata agaaaagaga cagtattagt ctctgttatg
     6121 gctgtgaata atgaagtagg atcgattcag cccatccagg aaatcagtga tcttttggat
     6181 gattttccta aaattcattt ccatgtagat gctgttcagg cgattggaaa agtaccattc
     6241 tcgcaatggt tgacagaaag agttgatttt gcaacatttt cagctcataa attccatggc
     6301 ccacggggaa ctggtttcat ttattggaaa aagggtcgtc gtttagcacc attactgaca
     6361 ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa atgttccagg tattgtggct
     6421 atggttaaag ctttacgttt atcattagag ataaaggaac aaaaacctga gcagacacaa
     6481 cgcttaaaag tagctctaat caatgcgttg aagacgtatg agaaagtgac gattttttca
     6541 gaaggaactg actatgcacc gcatatttta tgttttgctc taaaaggaat tcgcggcgaa
     6601 gttctcgtcc atgccttaga agaaaaacaa atatttgttt ctactactag tgcctgctcc
     6661 tcacgtaaac atatggcaag cagtacattg cacgcaatgc atgtaccaaa tgatctggct
     6721 acatctgctg ttcgaatcag cttagatgaa aacaattcgc ttgcagaagt cgagcaattt
     6781 atgattattt tcaagcagct ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt
     6841 cttgtgaaat acagtgaaat tatggtccgt tatggggaac tttcgacaaa aggaaagaat
     6901 cgaaaaagct ttatcatgca actagcacaa aatgtcagac aagctttgaa agactttcca
     6961 gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt tattgaatgg cgcagacggg
     7021 cagctagtta ttccaaaact agcaaaaata tttgggatcc aaaatttctc tccaagtatt
     7081 cgtgtagaaa aagatgtcga agttttgaaa caagctgttc aagacatcat gaaagaaatt
     7141 tatactggga aagaaacatt taaaattatc gctaaaagaa gcgatcacca atttgaattg
     7201 acaagtaatg aattgaatca aacattaggt aatgctgttt ttgatatttt cccgcatatc
     7261 caagttcaaa tgcgtcaacc agatattcca ttacgtgtag agattcggcg tgatggcgct
     7321 tatctatcgt atgaaacgat caagggagct ggaggattgc ccgtaggaac aagcggaaaa
     7381 ggaatgctta tgctttctgg ggggattgat tctcctgtag cagggtactt tgccatgaag
     7441 cggggagtgg aaattgaagc tgttcatttt gctagtccgc cgtatacaag tgaacaagca
     7501 ctgcaaaaag caaaagatct aacagccaaa ctagcacctt atgtgggaac gatccatttt
     7561 atcgaagtgc catttaccga gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat
     7621 tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt
     7681 catggtttag tcattttgaa tggtgaatca ttaggtcaag tggcttcaca aactttgcaa
     7741 agtatggtgg ctatcaatga agtaacgaat acgccaatca ttcggcctgt agcgacaatg
     7801 gataaacttg aaatcattga agtagcagaa cagatcgata catttaatct ttctatccag
     7861 ccttttgaag attgctgtac gatttttgct cctccacagc caaaaacacg ccctcaactg
     7921 gaaaaggtcc atcaatatga agagcgttta gcaatagatg aaatgatcca acgagcacta
     7981 gctggcttga agatggaaga gatacaagca gaatcgtcta atgaaaaaac tgaagaattt
     8041 tcagaattgc tataagaggt tatgaaaata gtgttttgac cagagtatta gagaaacaaa
     8101 acgaaaaaat agctccatgt attttaccgt ttttgaacta atgtcgaaga ttagctgatt
     8161 gaataccatt tatcaagaag ttatggaaaa gccggttctt ttttactaac tgaaaaataa
     8221 aattttattt agagatcaag ctacagttga taaatgatat aaaaaagaga atttcccatg
     8281 aaatagcgag gttgtgacag aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa
     8341 ttgcttttca aatttttgtg aaatttcggc ttatttttcg aagaagttac ttctggaaca
     8401 ccgtgtatca gaggttgcga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     8461 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatg
//
LOCUS       DAXWLG010000111         8468 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXWLG010000111 DAXWLG010000000
VERSION     DAXWLG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.112"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     regulatory      <1..148
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            296..853
                     /locus_tag="ACGE4T_002000"
                     /pseudo
     CDS             296..853
                     /locus_tag="ACGE4T_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            911..2950
                     /locus_tag="ACGE4T_002001"
     CDS             911..2950
                     /locus_tag="ACGE4T_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8387245.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLYAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3059..4210)
                     /locus_tag="ACGE4T_002002"
     CDS             complement(3059..4210)
                     /locus_tag="ACGE4T_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8387246.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4364..4894)
                     /locus_tag="ACGE4T_002003"
     CDS             complement(4364..4894)
                     /locus_tag="ACGE4T_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8387247.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4993..5343)
                     /locus_tag="ACGE4T_002004"
     CDS             complement(4993..5343)
                     /locus_tag="ACGE4T_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFF8387248.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5500..7029)
                     /gene="ahpF"
                     /locus_tag="ACGE4T_002005"
     CDS             complement(5500..7029)
                     /gene="ahpF"
                     /locus_tag="ACGE4T_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFF8387249.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7039..7602)
                     /gene="ahpC"
                     /locus_tag="ACGE4T_002006"
     CDS             complement(7039..7602)
                     /gene="ahpC"
                     /locus_tag="ACGE4T_002006"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFF8387250.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7746..8408)
                     /locus_tag="ACGE4T_002007"
     CDS             complement(7746..8408)
                     /locus_tag="ACGE4T_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8387251.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
BASE COUNT         2851 a         1633 c         1553 g         2431 t
ORIGIN      
        1 ggctcctata caaacatagg ctgttgccca gaaacgtcga aagtcgccaa tgggtaaaac
       61 agaaactgtt ggattaaggc tttttccaat agctaagaat gttttcttta cgttgtatag
      121 tgctaaaact cggacgatga gatgatgcta ggtcagaatt cttgttcgaa aaggtttctg
      181 accttttctt tatatttttg tgactgactt atttgatgag acaaactcgc tcctcacctg
      241 acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa agatcgattg gtggaggtcg
      301 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      361 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      421 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      481 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      541 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      601 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      661 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      721 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      781 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      841 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      901 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      961 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
     1021 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
     1081 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1141 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1201 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1261 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1321 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1381 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1441 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1501 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1561 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1621 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1681 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1741 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1801 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1861 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1921 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa tagcttttga
     1981 cagtctagga ggaaaagctg catcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     2041 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     2101 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2161 ttatgctcct ggatctacgt ttaaaatgat tacagcagca atcggtctcg acaacggcac
     2221 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2281 gggatctttt aaaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2341 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2401 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2461 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2521 tggatatgga cagggcgaac ttctaattac tcctatccag caagcagcaa tgtattctgt
     2581 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2641 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2701 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2761 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2821 cttgtatgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2881 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2941 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     3001 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     3061 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3121 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3181 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3241 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3301 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3361 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3421 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3481 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3541 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3601 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3661 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3721 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3781 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3841 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3901 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3961 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     4021 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     4081 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4141 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4201 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4261 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4321 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4381 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4441 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4501 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4561 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4621 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4681 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4741 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4801 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4861 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4921 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4981 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     5041 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     5101 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5161 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5221 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5281 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5341 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5401 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5461 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5521 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5581 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5641 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5701 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5761 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5821 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5881 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5941 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     6001 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     6061 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6121 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6181 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6241 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6301 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6361 agctgcacta ctagctgctg gtccaccgcc aatcactaat acatcataag gttctttttc
     6421 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6481 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6541 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6601 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6661 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6721 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6781 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6841 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6901 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6961 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     7021 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     7081 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7141 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7201 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7261 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7321 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7381 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7441 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7501 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7561 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7621 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7681 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7741 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7801 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7861 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7921 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7981 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     8041 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     8101 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8161 tctcggacaa agcgagcatt ttctgcagaa acaagattaa tttttgaaac atacgtacca
     8221 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8281 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8341 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgaga ttgcaagctg
     8401 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8461 aaaaacaa
//
LOCUS       DAXWLG010000112         8401 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXWLG010000112 DAXWLG010000000
VERSION     DAXWLG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.113"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            254..733
                     /locus_tag="ACGE4T_002008"
     CDS             254..733
                     /locus_tag="ACGE4T_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFF8387252.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACGE4T_002009"
     CDS             766..2994
                     /locus_tag="ACGE4T_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8387253.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACGE4T_002010"
     CDS             3123..4766
                     /locus_tag="ACGE4T_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8387254.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACGE4T_002011"
     CDS             complement(4839..5384)
                     /locus_tag="ACGE4T_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFF8387255.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACGE4T_002012"
     CDS             complement(5407..5892)
                     /locus_tag="ACGE4T_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8387256.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACGE4T_002013"
     CDS             complement(5882..6694)
                     /locus_tag="ACGE4T_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFF8387257.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACGE4T_002014"
     CDS             complement(6833..8365)
                     /locus_tag="ACGE4T_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFF8387258.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
BASE COUNT         2840 a         1598 c         1653 g         2310 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 a
//
LOCUS       DAXWLG010000113         8372 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXWLG010000113 DAXWLG010000000
VERSION     DAXWLG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.114"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            208..2250
                     /locus_tag="ACGE4T_002015"
     CDS             208..2250
                     /locus_tag="ACGE4T_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8387259.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="ACGE4T_002016"
     CDS             2261..2881
                     /locus_tag="ACGE4T_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFF8387260.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="ACGE4T_002017"
     CDS             2893..3351
                     /locus_tag="ACGE4T_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8387261.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="ACGE4T_002018"
     CDS             3370..3648
                     /locus_tag="ACGE4T_002018"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8387262.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="ACGE4T_002019"
     CDS             3673..5040
                     /locus_tag="ACGE4T_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8387263.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE4T_002020"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE4T_002020"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFF8387264.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="ACGE4T_002021"
     CDS             6227..6718
                     /locus_tag="ACGE4T_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387265.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="ACGE4T_002022"
     CDS             7161..8360
                     /locus_tag="ACGE4T_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8387266.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT         2571 a         1384 c         1804 g         2613 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata ct
//
LOCUS       DAXWLG010000114         8002 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXWLG010000114 DAXWLG010000000
VERSION     DAXWLG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.115"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            382..570
                     /locus_tag="ACGE4T_002023"
     CDS             382..570
                     /locus_tag="ACGE4T_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFF8387267.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACGE4T_002024"
     CDS             648..1661
                     /locus_tag="ACGE4T_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8387268.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="ACGE4T_002025"
     CDS             complement(1728..2762)
                     /locus_tag="ACGE4T_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8387269.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="ACGE4T_002026"
     CDS             2877..5102
                     /locus_tag="ACGE4T_002026"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8387270.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="ACGE4T_002027"
     CDS             5120..7003
                     /locus_tag="ACGE4T_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFF8387271.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="ACGE4T_002028"
     CDS             6984..7958
                     /locus_tag="ACGE4T_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFF8387272.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2583 a         1461 c         1696 g         2262 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aa
//
LOCUS       DAXWLG010000115         7969 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXWLG010000115 DAXWLG010000000
VERSION     DAXWLG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.116"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            119..1210
                     /gene="phnW"
                     /locus_tag="ACGE4T_002029"
                     /pseudo
     CDS             119..1210
                     /gene="phnW"
                     /locus_tag="ACGE4T_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            1203..1988
                     /gene="phnX"
                     /locus_tag="ACGE4T_002030"
     CDS             1203..1988
                     /gene="phnX"
                     /locus_tag="ACGE4T_002030"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFF8387273.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(2334..4025)
                     /gene="argS"
                     /locus_tag="ACGE4T_002031"
     CDS             complement(2334..4025)
                     /gene="argS"
                     /locus_tag="ACGE4T_002031"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFF8387274.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4447..5394)
                     /gene="arcC"
                     /locus_tag="ACGE4T_002032"
     CDS             complement(4447..5394)
                     /gene="arcC"
                     /locus_tag="ACGE4T_002032"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFF8387275.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5509..6528)
                     /gene="argF"
                     /locus_tag="ACGE4T_002033"
     CDS             complement(5509..6528)
                     /gene="argF"
                     /locus_tag="ACGE4T_002033"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFF8387276.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6619..7848)
                     /gene="arcA"
                     /locus_tag="ACGE4T_002034"
     CDS             complement(6619..7848)
                     /gene="arcA"
                     /locus_tag="ACGE4T_002034"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFF8387277.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2275 a         1630 c         1459 g         2605 t
ORIGIN      
        1 tttattcaat tttttcaaca ccttgattga atcacaataa tcatcattta tattggatgt
       61 taacaagagc agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat
      121 gtataaatta ttaactcctg gaccattgac gacttctgaa acagtgaaaa atgctatgct
      181 gatcgatcat tgtacttggg atcaagaata caaagaaatt acacaatgga taagaaaaaa
      241 attattggat cttgctcaag tatcggaagg aatatacaca acggtcttaa tgcaaggtag
      301 cggctccttt ggggtagaat cggtattaag ttctgtgatc aggccagaag atactgtact
      361 gatttgttca aacggcgcat atggacaacg aatgatcaac atgtgcgaat gtctgaaact
      421 gaactatacc gtttatgagg tatcgtaaca tcaaatcctc caagctcatg tgattacaca
      481 gctgattgaa gagaatccct ctatctccgc actagcaatg gtccatagcg agactacttc
      541 cggtattttg aataatttgg aagagattgc tgctgtcaca caagaaaaag atcttttgtt
      601 tatagtggac gcgatgtcaa gctttggtgg gataccgatt cgtgtagccg aactagggat
      661 tgattttttg atttccagct caaacaaatg tattcaaggt gttccaggct tttcttttgt
      721 acttgctaat aaagcggaac tagaaaagcg aaaaaatgtg agtcgaagtt tgtcacttga
      781 tctatatgac cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc
      841 tacccatacg gttttggctt ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat
      901 cgaaactcga tattcacgtt atctcgccaa taatcatgta ctacgacaaa aaatgacaga
      961 gatggggttc cagcctttca ttcaagaata tcaggggcca ttcatcacct cttttcttta
     1021 ccctaaggac tggcgatttg attttaatca tttttatcag tatttaaagc aaaacggatt
     1081 tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa atattggcga
     1141 gatctatccg gaagatattt tggaactgac agcaattatc aaagactata aggagaacgt
     1201 taatgattaa aactgtaatt tttgattggg caggaacaac tgtggacttt ggctgtatgg
     1261 ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg aatccaactg actgacaaag
     1321 aaatccgcga accgatggga aaattaaaat gggatcatat ccaacagctt ctttccctcc
     1381 cttctgtcaa aaaacaatgg atagctattt atggacaact acctcaggaa aaagaggtgg
     1441 aggaattgta tcaacaattt gaacattatc tgtttaaaga tttggtcaag cattctacct
     1501 tgaaacccga cacattggaa acgatcgaac ggttgagaaa cgctgggatc aatattggtt
     1561 ccacgactgg atttactgct cctatgatga caatcgtagc agaaaccgca aaaaaagaag
     1621 ggtattcacc agacttcatg gcaacttctg aagacgtcga agggtatggt cgaccgtttc
     1681 cttatatgat ccataaaaac atgcagtact tcaaaaacaa gtcggttgat gaagtaatca
     1741 aagtcggaga tacggtctca gatatccagg aagggaaaaa tgccggagtg ttgacagtag
     1801 gtgtaattga aggtagttcg ctaatgggga tttcttatga tgagtaccaa aaattagaga
     1861 tggggaaaaa agatcgttta aaacagtctg ttgctcttag attccaacaa gctggcgctg
     1921 atatttgtgt aaacaattta aaagagttaa cagaatacat tttgtacgct gacaagaatc
     1981 cagaatgact tttatacaaa atcagaggtc gtgaaaaagc tgtactgcat caagtaataa
     2041 gaacagccaa acaaaaatag ctcctcatat ttttcgtttg gttaggctta ttaccgcaga
     2101 tgcaagcttt tgaacaccgt ttattcgaaa aaaaaaggga gctgtgacac gacttttgtc
     2161 acagtcccct tatttacaaa caaacagtgt ttaagagaca acctctccac taaacggtgt
     2221 tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa gctattttct
     2281 cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt ttcttacatc
     2341 ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac agtcactgca
     2401 taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt cgtatgagca
     2461 tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac tgacggttcg
     2521 tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt aaccacttcc
     2581 cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa tcctgctttt
     2641 tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt ttctccttca
     2701 aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg
     2761 tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt ttccaaatca
     2821 gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa tacttcttct
     2881 aataagacga tctttccttt acgagttgat aattttttac caccttgcgt gatcaaacca
     2941 aatggaatat ggcgcatatc gtcagaccag tcaaagccca tttccttcaa gacagctttc
     3001 aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg tttgaagtcg
     3061 taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg
     3121 tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac gatctctgca
     3181 cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc atccatcttg
     3241 tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag atcatagatt
     3301 ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat agcctcttca
     3361 tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc tggttctgtt
     3421 tcagctactt catggaattg gacatataaa cgaagcaatt cattgattgg ttctgcttta
     3481 acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc
     3541 cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat aatgaaaccg
     3601 atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt ggcaatatta
     3661 ggagaagaca tgtcgattgg tactgttcct tgattcccga tattgctatc cccataatgt
     3721 tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt attcatgaaa
     3781 aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc gattttttct
     3841 gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct
     3901 ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg ttcgacttgt
     3961 tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag atctttatta
     4021 ttcatcttta ttcctcctaa agtgctccct tgattgttaa acaaaaaaaa tctctagcga
     4081 cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac tccaattgtc
     4141 ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa aacagaattc
     4201 tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga attcgctagc
     4261 caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa ctttcttgaa
     4321 ttatatcaga aaaaaaaacg aaaggctagt cgtttctgat aaaaaaacaa tccagatggc
     4381 ctaaaggaga agaaatacca cctggatagc tataaatcat ttcgtaaata cgaaagactt
     4441 aacaaattaa tcagctacga taatcgttcc ttcttcagaa gctaaaagat tctcgatatt
     4501 ttctaaagaa gtgatgattg ccttagcgtt tggttttgct tcaacaaatg caatagctgc
     4561 ctctactttc ggaagcatgc ttcctggagc aaattgcttt tcatcaatgt attgtttcat
     4621 ttctgaaaca gtaacagttt ctaatttctt ttgatctggt ttttggtaat tgacatacac
     4681 attgtctact cctgtcaata cgatcagcaa atccgcatcg atgatttcgg ctaatttttc
     4741 tgaagcaaaa tctttatcga ttacggcttc tcgtccttct aatccagtag ctgtttctac
     4801 aacaggaatc ccaccgccac caacagagac tgtgataact ccttgatcga ccagcgtttc
     4861 gatgacacgt gcttctttaa tcgagatggg tttaggggaa gcgactactt ttctccatcc
     4921 acgtccagca tcttctttga atgtcacagt ggagtcagca ttcatttgtt ctttggcttc
     4981 ttcttccgtg tagaatggac cgactggttt acttggattt ttgaatgaag gatcattttc
     5041 atccacaata acttgagtga ctaaagaaac cacatcttta tcgatccctt tttccttcag
     5101 tacttcgccc atggcatttt gtaaccagta accgatagat ccttctgtca ttgcaacaca
     5161 tgtatctaaa ggcattgccg gtgttttctc agaatcagca gcttgctgct gaatcaatag
     5221 gttaccgact tgcggaccat ttccgtggga gatgatcaat tcatctcctt gttcgataaa
     5281 tttcactaaa tactttgctg tttccattaa cgcttcttgt tgggcttttg cacttgcatc
     5341 cgtagacaag attgcattac cccctaaagc gacaactact tttcgtttgg ccatttcatt
     5401 cgtcctttct tcagttctac cgaattttct agataacaaa acgagccagc gttttgtgat
     5461 tttccctcaa tgctgactcg ttttttcgtg agatgaatgt ggttatgctt aaacgcgagg
     5521 gataaacaag ttacctaatg ttgctgccat gatcgctttg attgagtgca tacgattttc
     5581 tgcttggtca aattgacgag catatttgct gcggaagact tcatctgtta cttccatttc
     5641 agtgatgcca aagcgttctt tcatttgttc accgtaaaca gtgtttgtat catggaaagc
     5701 tggcaagcag tgcaagaaga tcaaacgatc tgtgttgtgt gttttttcta ccatttccat
     5761 attgatttgg taaggtttca acaatttgat acgttcttcg aatttatctt cttcacccat
     5821 tgatacccat acatcagaat aaaggacgtc tgctccgtct acacctttat caacatcgtc
     5881 ggtaatcatc aattgtgcac cggatttttc agcaaagcct tctgccattt tgacgatttc
     5941 ttcttcaggt tgtaattctt ttggtgcaac gatacgcata ttcgctccta aaattgcacc
     6001 tgtgactaat aaagagttag ccatattgtt tcgaccatct ccgcagtaag caacagtgat
     6061 accttctact gttccaaagt tttcttggat agttaaaaag tcagcgatca tttgtgttgg
     6121 atgccattca tcagtcaagc cattccaaac tggaacacca gagaattctg ctagttcctc
     6181 taccattttt tgactgaatc cacggaattc gattccatca aacatacgac ctagtacttt
     6241 agctgtatct tctgtggatt cttttttacc taattggata tcatttgcac ctagatattc
     6301 aggatgtgca cctagatcga tcgctgcagt agtaaatgca gaacgagtac gtgtagatgt
     6361 tttttcaaat aaaagcgcaa tattttttcc ttctagatag tgatgtggga taccacgttt
     6421 tttcaagtct ttcaagtgtt ctgaaaaatc gatcaaatat tgtaattctt cttttgtaaa
     6481 gtctttttct gctaataagc ttcttccttg gaatactgat tctttcatat taaacattct
     6541 cctttagatt gtttttattt aagattaaag aggaaatccc gacggcagct gacagtaaga
     6601 ccgccgtcag gaaacaaatt acttcaagtc ttcacggaca agtggttggc tcatgcagcg
     6661 tggaccacca cggcctcgtg acaattcact tgaattgatt tctaacactt tgatgccata
     6721 gctacgaagc aattcgttcg atacataatt tcggttataa gtgactacaa cacctggagc
     6781 gattgccaat gtgtttgatc catcattcca ttgttctcga ggtgcaacga tctcatctcc
     6841 attaccagtt gggatcagta caagatcatc taatccaaga gcagctttca tcgtttcgtg
     6901 taagttatct gaatgagtaa tcttgatatc atcaccatct ggttcgattg taaagacatc
     6961 catcttaccg tctttacttt gaattgctgg atggatagtg aatttatcgt gatctaccat
     7021 tgtaaatacg gtatccaaat gcatcattgc acggttgttt ggaattttga ttgctaacac
     7081 tttttcaaat cctgaatttt ttgcgaatag gtggcgtgct aatttttcta aagctttcgc
     7141 attagttcgt tgagaaatcc caacagctac tactttatcg cttaagatca attcgtctcc
     7201 gccttcgata tgatctggat tttcacgatc caaccatact tcgattcctt tatttgcaaa
     7261 tcttggatga tgtttcaaaa tatattctgt aaacatcgat tcacgacgac gtgcttcaaa
     7321 agtcatctta ttgactgtca tcccatttcc cattgatgcg gaagggtcgc gtgtaaagta
     7381 caagtttggc attgggtcca tgtaaaatgg atagtcatcg tctgctgaca gatcgtatag
     7441 actgctagaa cgtacttcaa tatcttttgt acgaacccca gccatgattt tatctaccat
     7501 ttcttgcgtt tccattgata gaaggaactc atgaagaccc tctcttacag cattcgatgc
     7561 aatatgagat tcatccaaca ttttattcaa gaattgttct ttgacattgc ctgcgtcaat
     7621 cgcttctgcc gctaattttt ccaagtaaag agtctcgacg ccttcatttt gcaatgtctt
     7681 tgcaaagttg tcatgttctt cttgagcaat cggaagatat ggaatatcat cgaacaacag
     7741 acgatccata atatctggtg tcaaattttc cacttcttgt ccaggccttt tcagtaaaac
     7801 tgtctttaat ttccctattt cagagaaaac gtgaataggt ttatccatgt tgtgttgcct
     7861 ccttcttttt gattacatgt ataggatatc ctccttttgt aatcactttc ataattttgt
     7921 ctcattcaaa atgagacatt cctcacatta atattcgata attaaataa
//
LOCUS       DAXWLG010000116         7900 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXWLG010000116 DAXWLG010000000
VERSION     DAXWLG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.117"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..875
                     /locus_tag="ACGE4T_002035"
     CDS             <1..875
                     /locus_tag="ACGE4T_002035"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFF8387278.1"
                     /translation="DIINKKGGENNMPRKPLSEEDKKARSIIANNLKKITHGYTRAQL
                     SDMTGIPVTTLSGYFTEKSTISMENAELIAKAFNVDKSDIDPRYSREYNYYDLKKEVL
                     DELQENNKSFVADKFVELAEKANAAGMTLNRTKTDRGNIMTVLSSDIQEYNFFDTSVA
                     AGIPSSVEAFDQDHIEQIAIPDYAMGKYAGYQDVFFTKINGDSMNNVIPDGSLIAVKK
                     IESCDELKDGDIVVFSNDNEFSVKRYINDQQNNRIIFRPDSSNISFTDIVVNYDDTKY
                     VQIYGKVVVYIVQL"
     gene            896..1096
                     /locus_tag="ACGE4T_002036"
     CDS             896..1096
                     /locus_tag="ACGE4T_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387279.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            1183..1488
                     /locus_tag="ACGE4T_002037"
     CDS             1183..1488
                     /locus_tag="ACGE4T_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387280.1"
                     /translation="MKNNVGQNIVICKNCGSNKVNVTTPGANGCAAFGVGFLLICIGV
                     WIPVIGWFVIVPIGALIALSSFIFPLFQKKYGVTCKDCEHKFYISKEQYKQYIKAVK"
     gene            1593..2732
                     /locus_tag="ACGE4T_002038"
     CDS             1593..2732
                     /locus_tag="ACGE4T_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8387281.1"
                     /translation="MWVEQTKDGRFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSFLKVPMMMKHVSKHISDD
                     TIVRNIDEKLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYENGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLTLHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(2821..4446)
                     /gene="groL"
                     /locus_tag="ACGE4T_002039"
     CDS             complement(2821..4446)
                     /gene="groL"
                     /locus_tag="ACGE4T_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFF8387282.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(4497..4781)
                     /gene="groES"
                     /locus_tag="ACGE4T_002040"
     CDS             complement(4497..4781)
                     /gene="groES"
                     /locus_tag="ACGE4T_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFF8387283.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            5006..5668
                     /locus_tag="ACGE4T_002041"
     CDS             5006..5668
                     /locus_tag="ACGE4T_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8387284.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(5744..7036)
                     /locus_tag="ACGE4T_002042"
     CDS             complement(5744..7036)
                     /locus_tag="ACGE4T_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8387285.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            7206..7835
                     /locus_tag="ACGE4T_002043"
     CDS             7206..7835
                     /locus_tag="ACGE4T_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8387286.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
BASE COUNT         2565 a         1521 c         1404 g         2410 t
ORIGIN      
        1 tcgatattat taataaaaaa ggaggagaaa ataacatgcc aagaaaacca ttatcagaag
       61 aagataaaaa agctcgctcc attatcgcta acaatttaaa aaaaattacg catggctaca
      121 caagagcaca attgtcagat atgacaggta ttcctgttac gacgctatcg ggatatttta
      181 ctgaaaaatc aactatcagt atggaaaacg ctgaattaat agctaaagct tttaatgttg
      241 ataaatctga tatagatcca agatattcca gagaatacaa ttattatgat ttaaagaaag
      301 aagttttaga tgaattacag gaaaacaata aaagtttcgt tgctgataaa tttgtagagt
      361 tagcagaaaa agcaaatgca gcagggatga ctttgaacag aactaaaact gatagaggaa
      421 acattatgac agttctatca agtgatattc aagaatataa cttttttgac acttctgttg
      481 ctgccggcat accttcttca gtcgaagctt ttgatcaaga ccatattgag caaattgcta
      541 ttcctgatta tgccatggga aaatatgcag gatatcaaga tgtatttttt actaaaataa
      601 atggagattc catgaataac gttattcctg atggatcatt aattgctgta aagaaaatag
      661 agagctgtga tgagttaaaa gatggagata tagtagtctt tagcaacgat aacgaatttt
      721 ctgtaaaaag atatataaat gatcaacaaa ataatcgcat catttttaga cctgattctt
      781 ctaacatttc tttcactgat attgtggtta attatgatga tactaaatac gttcaaattt
      841 atgggaaagt cgttgtatat attgtccaac tataattaat ttgagagggt ataaaatgaa
      901 aattggaatg agaaaaccga gtataaaaaa atccattagc gctagaacta ctggaaaggc
      961 aaaaagaaaa gtaaaaaaag cagttattcc tggttatggg aaaaaaggta gcggctggat
     1021 taaagaccca aaaaaggctg cttacaataa agtatacaag aaaactacat tcagtttttg
     1081 ggatttattt aaataaaaaa agcccgtgca gcaacacgga ctcgaaacct tatttctaag
     1141 attccccaat aaaaatcata tcacagaagt gaggaattaa aaatgaaaaa taatgtaggg
     1201 caaaatattg ttatatgtaa aaattgtggc agcaataaag tgaatgtcac aactccagga
     1261 gcaaatggtt gtgcggcctt tggcgtaggg tttttactta tttgtattgg tgtttggatt
     1321 ccagtaattg gttggtttgt cattgttcca ataggggctt tgatagcact tagttctttc
     1381 atatttccac tcttccaaaa aaaatacggg gtaacatgta aggactgtga acataaattt
     1441 tatatttcaa aagaacaata taagcagtac atcaaagctg taaaataaaa acacgcccca
     1501 ccgaccaaag caagcgtgtt taagtccaag aactacggta aagtaggtct atttgttatg
     1561 cctattatag caaatgatag gagatgttaa aagtgtgggt tgaacaaacc aaagatgggc
     1621 gattcaagtt tattgaaaga tatgtagatc cttatacaga aaaaacacga aaaaaatcta
     1681 caacacttac aagtaattca ccacaagcgt ggaaaaaagc tcagaaaatt ctagataaaa
     1741 aaattaaaga agcactcgaa aattacaata aatcagatat cacttttggt gagctgtata
     1801 aagaatggta tgaatattat aagcagcatg tcaaacgtac tagctttttg aaggttccaa
     1861 tgatgatgaa acatgtttct aagcacatca gcgatgatac aatcgttaga aacattgatg
     1921 agaaactcat taataagata attgaagata tgtatacgtt tggtgacctc tcactgaact
     1981 acacaaaaca aacaaaaaca actctatccg ttatgctaaa ctatgcaatc gatagaaaat
     2041 acattcaaag aaaccctgcg ctagcagtta aaatccaccc taaaaaagtg gaagaagaaa
     2101 aaagaaggct ttctatggat aaaaaatatc tggagaaaga agaaattgat cagatactga
     2161 aacagttata ttccaatcct cgcagaaaac tacacggcat aatagctgaa tttctatatt
     2221 tgactggttt gagatatggg gaattgctag ctttacaaat gaaggactat gagaatggga
     2281 aaatttctat taatggaaca ttggattaca catctgtgaa aatggataat gctataaaaa
     2341 caactccaaa aaatacttat tcgcaacgtg aagtgcaatt acccaatcgt gcaaaagaat
     2401 tgattgaaag tgtgatagct gacaatattc ttgcaggtag acccacagat cccgatcaat
     2461 atatatttat atctacaagt ggcactccgc ttacgctgca ctcatttaac gctatactcc
     2521 ataaagtaga ggaagagttg gaattagaaa aaagtctatc ctcacatata ttcagacata
     2581 gccacgtttc actattatct gaattgggtg tacctcttaa ggctatcatg gaacgtgtag
     2641 gacattctga tgcaaataca actctgtcta tttataacca tgtaaccaaa agagccaaac
     2701 aacaagtaat tgataaacta aatagccttt gatatttttg ccccttcttt gcccctttcg
     2761 cttaaaataa aacaaaaaaa gaagaaagaa ccctgatata tcaaggttct ctcttaaata
     2821 ctacatcata ccgcccatca ttgatggatc cattgcagga gctgctggcg ctgctggttc
     2881 tggtttatct gcaacgactg cttctgtcgt taataacaag gcagaaacgg aagcggcatt
     2941 ttgcaaggca gaacgtgtta ctttagttgg gtctacgata cctgcttcaa ccatgtttac
     3001 ccattctcca gtagcagcat tgaaaccagt gcctagttcc acgttcttca atttatcaac
     3061 aatcactgat ccttcataac cagcgttttc agcgatttga cgaattggtt cttctaaagc
     3121 acgaacaacg atcttgatac ctgttgccac gtctccttct gcttctacag cagaaacttt
     3181 gctgataacg ttcactagag ctgtaccacc acctgagacc attccttctt ctactgcggc
     3241 acgtgtagca ttcaatgcat cttcaatgcg taattttaat tctttcaatt ctgtttcagt
     3301 agctgcacca actttaacaa cagctacacc acctgctaat ttagctaagc gttcttgtaa
     3361 tttttcgcgg tcaaaatcag aagttgtttc agcaatttgg tttttgatca attgcacacg
     3421 agcttcgatt gcttcttttt ctccagaacc ttctacgatc gttgtattgt ctttatctac
     3481 aacaacttta gaagcatttc ctaagttttc gattgttaca tctttcaatt ctaaacctag
     3541 atcgtcagta attactgtac cacctgtcaa aatagcgata tcttcaagca ttgctttacg
     3601 acgatcgccg aaaccaggag cttttacagc tactacgttg aatgttccac ggattttgtt
     3661 caatacaaga gttggcaatg cttcaccatc tacatcgtca gcaataatca acaatggacg
     3721 tgattgttgc aagatttgtt ctaacaatgg cagaatatct tggatgttag agattttttt
     3781 gtctgtgatc aagatatatg ggttttctag aacagcttcc attttgtcgt tatctgttac
     3841 catatattgt gataagtaac cacgatcaaa ctgcatacct tctacaacat ctagttctgt
     3901 ttcgatccct tttgattctt cgattgtgat gacaccgtcg tttccaactt tttccattgc
     3961 atcagcaatc aaatgtccaa ctttatcaga acccgaagaa acagctgcta cttgtgcaat
     4021 ggcttctttt gaatcaacaa cagttgagat attatgcaat tcttcgactg ctgcttttgt
     4081 tgccaattcg atcccgcgac gaatgcctag aggattagca cctgcagtta cgttttttaa
     4141 accttcgcga acgattgctt gtgtcagaac agttgctgtc gttgtaccgt ctcctgcaat
     4201 atcatttgtt tttgatgcta cttctgatac aagttttgca cccatgtttt caaaatgatc
     4261 ttctaattcg atttcttttg cgatcgtcac accatcattt gtgatcaatg gagagccgta
     4321 agatttttct agtacgacat ttcggccttt aggtcctaat gttactttca ctgtatctgc
     4381 taatttatct accccgcgta acatagctgc gcgtgcgtct tctgcaaatt tcaattcttt
     4441 tgccatgatt gattcacctc attgattatt tttgattatt tttttatctt ccaaatttac
     4501 tcaacaatcg ccatgatatc ttttcctgcg acgatcagat attcttttcc ttcgtatttt
     4561 acttctgtac cggcgtattt ttcgaacata acttggtcgc ctgcttttac agcggcagga
     4621 actttttcac cgttttctaa caaacggcct tcaccgactg caacaactgt cccagtttgg
     4681 ggtttctctt tagcagcaga agctaaaacg attcctccga cagttttttc ttcttctttt
     4741 gcgacttcga taatgacgcg atcacctaat ggttttaaca cgataaatcc ctccgataac
     4801 tcttttttta atctgttagc actcttaacc ttcgagtgct aacctacatt tttaataata
     4861 ctcattctcc ctcttttttg caagctttta aatcaaagaa aagaaatttt attctgacag
     4921 ctgcttattg aaattatgat ataattgaaa tcaatcgata taagcttgcc ttgcatgctt
     4981 gttatccctt aaaaggagga attacatgtc tttaaaaaaa tatagcttcc tcagcatttt
     5041 ttgctatggt ctaattttta tctcaccctt gatcctagct tcgataggac ttgtaaaatc
     5101 tacgtctgaa ttgatcacag caactgcagt catgtatata ctaggcgccg ttgtcctagc
     5161 tattttttat tttaagcaac gtgaaccctt agcgatcgaa tcagcggtaa aaaaagcttc
     5221 tgctccaaag attgttattt atggattggt tggaatcttt gtcgcattga ttctgcaaag
     5281 catcgctgtc atgttagaat ctgtattgtt tggcaaagca accccttccg agaatacaca
     5341 aaatatcatc cagatgatca tggaagcacc cgcttttatt ttggcgacaa ctgttgctgg
     5401 acccatcatg gaagagttcg tattccgccg cagtatactg gggatcatca gtcgatattc
     5461 aaatttttgg gtcggagcag tgatcagctc tttgctgttt gcgtttgctc ataatgatgg
     5521 tcatctgcta atctactttt tcctaggttt cttcttcagc ctagagtaca aagcaactgg
     5581 tcggatctgg acatcgatga tcacacacgt aggaatgaac acattagttg ttcttgttca
     5641 attagctgtc caaaaagggc tagtataaac atgactataa atactcctgt catcctagat
     5701 tggtgatttt cccaatctaa agcgacagga gtatttttgt tttttagtcg ttcatctttt
     5761 ctgctgctcc atcatttttt gtctgtcttt cctttttgca gtttgcagaa tctatttttt
     5821 tcaaaaatgg gaaataaatg aaaaaactaa ttgacaacac gataccttgt aaaatcgctg
     5881 caggcactcc gcctactaag aaacccgaga ggatcggcgg tgttgtccaa gggacttgga
     5941 ctgctgtaaa taaaggaacg aatccaaaat ataaagcgga ataggtaatc aatcctgaaa
     6001 cgaccggtgc gaaaataaaa ggaacagcca tcatcgggtt catcacgatc ggtgtggaaa
     6061 aaataattga ttcattgatg ttgaaaaagg ctggtaaaag agcaagattt cctaattctc
     6121 tgtatttctt cgatttcgca aaaaccgtca taaaaaatac agcccctaac gtcaatccag
     6181 cacccgtaac cgtcatgaac tgatcgagaa attgctgagt aacgatatgt ccgccatttg
     6241 ccaccgtcaa ctcttgtcct gaggctaaga tttctgcatt cgctaatgaa ttcgcttgca
     6301 agacgggact aaccaaccca ttgaccatcg cagatccatg aagaccaaat aaccataaaa
     6361 acggaacaaa aaaacctaat aaaagcacac caaacaacgt gtctatgaac gattgaatgg
     6421 gaagctggac atatgtccat actccttgaa caacagtcat tctgaacaac tgttgaaaaa
     6481 ccgctgaacc gataagcgta aaagtgacta taacaatgat tggaatcaat gaagtaaaag
     6541 actgccaaac attttcaggt atcttctctc tttgcttcca ccctttcgca agtcttacta
     6601 aacgtgcata gccccatccg atgatgagcc cagttgtaac tgctcctagt atcccttcag
     6661 cacctgtcca gacagtatct atctgagtat ttagtggctg aataagcagt agataagcag
     6721 acaaggctgt tatgccgact ggtaagcgat cgatgtcttc ttcttttgca taagcgtatg
     6781 caatggcaaa aacgatccaa aaggctatca catcaaatgt agcctggtat acttgtgtaa
     6841 aatacataga aaatcctgtt ttgtctatcc actgagcgat gtaatccaca ggaaaatgag
     6901 caaataagaa aaacagcgag ccaacaataa ttattgggat cgtgtaagtc attccattac
     6961 gcaaagcagt taagggtcta agattcacaa cagatgttat cattggtaaa aaggcacgat
     7021 ctaaccactc tttcatttct tatctccttc aacttctatt tttatcaatc agttttctta
     7081 atttaactat acccaatgat cacgaaccat gaaaggaatg cgcactgagc ttttcaaaaa
     7141 tcggattctt tgacgaatat tcagcaactg cttacaatgg acaggaataa aaaaggaggt
     7201 tttttatgaa aattgcactc attggagcta ctggccatgc aggttcattt attttagacg
     7261 aagcattgtc cagagattta gacgtgacgg caatcgtgcg tcatccagaa aaattgccat
     7321 cagatgttcc gtttatacaa aaggatttat ttgagttgac aactactgat cttgcatctt
     7381 ttgatgttgt cgttgatgct ttcaacgctc ccaaaggaaa agaagaaatg catcaaactt
     7441 ctttgtccca tttgattgag atgcttgaag gaaccgatac acgcttaatt gtcgttgggg
     7501 gtgcctcttc gttattctta gatacagaaa agacgactcg tatgattgac caagtaccta
     7561 aaaatgcacc cttctatcca acagctttca atatgtacga aggattgtta aaattgaaac
     7621 aatctaaaaa tctacattgg acttatatca gtccagcttc tttatttgat ccacatggca
     7681 aacgtacagg tacttatttg ttaagtgatg attatttaca aagaaacgct gctggcgaca
     7741 gtacgatcag catggcagat tacgcgattg cattagtaga tgagatactg gatgagaaac
     7801 atgaaaagca gcacatcagt gtagtcagtc gataatacgg ttattttttt aatatgctaa
     7861 aaaaataaaa atagaagata aaaccaaccc tgattgattt
//
LOCUS       DAXWLG010000117         7889 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXWLG010000117 DAXWLG010000000
VERSION     DAXWLG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.118"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(244..756)
                     /locus_tag="ACGE4T_002044"
     CDS             complement(244..756)
                     /locus_tag="ACGE4T_002044"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFF8387287.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(770..3067)
                     /gene="recJ"
                     /locus_tag="ACGE4T_002045"
     CDS             complement(770..3067)
                     /gene="recJ"
                     /locus_tag="ACGE4T_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFF8387288.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3209..3652)
                     /locus_tag="ACGE4T_002046"
     CDS             complement(3209..3652)
                     /locus_tag="ACGE4T_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFF8387289.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3653..4441)
                     /locus_tag="ACGE4T_002047"
     CDS             complement(3653..4441)
                     /locus_tag="ACGE4T_002047"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8387290.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4438..5379)
                     /gene="rnz"
                     /locus_tag="ACGE4T_002048"
     CDS             complement(4438..5379)
                     /gene="rnz"
                     /locus_tag="ACGE4T_002048"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFF8387291.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5556..6143)
                     /locus_tag="ACGE4T_002049"
     CDS             complement(5556..6143)
                     /locus_tag="ACGE4T_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFF8387292.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6364..7677)
                     /gene="obgE"
                     /locus_tag="ACGE4T_002050"
     CDS             complement(6364..7677)
                     /gene="obgE"
                     /locus_tag="ACGE4T_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFF8387293.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         2127 a         1835 c         1349 g         2578 t
ORIGIN      
        1 tacgggggtg tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc
       61 cagtttttcg gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct
      121 ttgcttgttt ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact
      181 tttgagggtc gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc
      241 tgcttaatat tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag
      301 atccatcaat tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag
      361 ttcgatcgtc gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg
      421 ttgacctggt ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc
      481 ataagtcact tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc
      541 gactcctaac tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac
      601 taccatatca attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata
      661 agcgtcacca tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata
      721 gtcgggaata ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta
      781 accatttctt tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat
      841 tgagccgtcg ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt
      901 tttgtaaaac accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa
      961 aaattaataa tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta
     1021 tatcaattcg ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc
     1081 ctaccccatt caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg
     1141 aataagaccg aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta
     1201 gagagtttgg ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa
     1261 tcgcagcttc ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat
     1321 aaaggctacc ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata
     1381 cttgcagacc acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc
     1441 attcattgat cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt
     1501 gagtaccaaa accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt
     1561 tcagatgctg ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata
     1621 aaaactgagg caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc
     1681 gctctactgt tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca
     1741 tcaagtcaat ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat
     1801 tttcacttgg catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat
     1861 ctcgcatctg gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt
     1921 tagctgatcc atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta
     1981 ttttcccagc aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga
     2041 tatgattctc cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt
     2101 ttctttcttc attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa
     2161 acgtcgcaag taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc
     2221 tgggagcaat cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt
     2281 cttcaaatag cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg
     2341 aaacaaggat tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta
     2401 ctaaatccaa aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta
     2461 ctccgactcc tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa
     2521 tcgcataagc gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta
     2581 caccagcttg ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga
     2641 tgatcaactg gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc
     2701 catgctcaaa tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt
     2761 ctttcattac cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt
     2821 tttctccact gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca
     2881 gatacggatc atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct
     2941 catggtaacc tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga
     3001 tcatttggag aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt
     3061 gtctcacttt ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca
     3121 gaaagaaaga ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt
     3181 ttgtacgacc atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg
     3241 attgagtttt ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta
     3301 actccgcctg ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt
     3361 cttcttttgc ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag
     3421 tcagctgttt gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa
     3481 tcagtgcacc aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag
     3541 tgaagccaaa acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca
     3601 aaactaaaac aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt
     3661 tgttaaaaat tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg
     3721 atgcaacttc taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga
     3781 cgatcatttt tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc
     3841 ttccgcttgg atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg
     3901 taacagctac gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg
     3961 caaatttcgt ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg
     4021 cgatattaat gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca
     4081 aaaccatcat tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg
     4141 taaggaagtc ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga
     4201 cttcttccat caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct
     4261 tgaaagcata agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa
     4321 atgccattct tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga
     4381 tttgtgcgcc cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca
     4441 tgcctcttcc tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt
     4501 gttttcaaac acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct
     4561 cgcactgata tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc
     4621 ttgatgagtc gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt
     4681 gctttcatgt accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct
     4741 cgtatcgcct aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc
     4801 attgatcgtc cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag
     4861 tggtccagct ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc
     4921 atgttcaacc acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac
     4981 agaaaattgc tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg
     5041 ataacttaaa cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt
     5101 tggcccgtaa atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc
     5161 gggaagtcca aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct
     5221 cggacgaata gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca
     5281 aacttcattg cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc
     5341 aggaacacct gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa
     5401 gttcctcttt aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt
     5461 caaattaaaa ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag
     5521 cagcgaaccc gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca
     5581 ttgtcgttgg aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca
     5641 tgattttcag cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga
     5701 gtaatttctc cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca
     5761 tcagcaatca agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac
     5821 acggtctggg catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca
     5881 aaccatccta ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat
     5941 ccacccgtag aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga
     6001 tgtgtcccat taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca
     6061 gcatgtaact ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt
     6121 aaaatgatta ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt
     6181 tacctttaat tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg
     6241 tgacaaaagt cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac
     6301 ttctgtcaca acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct
     6361 tgcctattcg acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc
     6421 accgcgtgcc cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat
     6481 cactgtttca tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga
     6541 taagatccac gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa
     6601 tccatagtgt acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac
     6661 ttctaacaaa tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga
     6721 aattggaaat actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc
     6781 tttgaatttc actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat
     6841 ttgcggacgc tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata
     6901 gtcttcataa ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg
     6961 tgttctttcg atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc
     7021 aatcaagcct ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc
     7081 tagattaggt acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat
     7141 aacggatagt aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa
     7201 tactttcaat tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc
     7261 cggtgcagga ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt
     7321 tgccacaact agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc
     7381 atctcggaca gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg
     7441 catcccttta ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa
     7501 atccatcaat gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc
     7561 gtctccgcca gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc
     7621 gtctccgccc ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat
     7681 taagtcctcc tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag
     7741 tcttacttat tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt
     7801 accttcttct ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata
     7861 attttttgac aaaatattct ttgcgtagc
//
LOCUS       DAXWLG010000118         7785 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXWLG010000118 DAXWLG010000000
VERSION     DAXWLG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.119"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(65..493)
                     /locus_tag="ACGE4T_002051"
     CDS             complement(65..493)
                     /locus_tag="ACGE4T_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8387294.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            585..1445
                     /locus_tag="ACGE4T_002052"
     CDS             585..1445
                     /locus_tag="ACGE4T_002052"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFF8387295.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(1537..1887)
                     /locus_tag="ACGE4T_002053"
     CDS             complement(1537..1887)
                     /locus_tag="ACGE4T_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFF8387296.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(1902..3629)
                     /locus_tag="ACGE4T_002054"
     CDS             complement(1902..3629)
                     /locus_tag="ACGE4T_002054"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFF8387297.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(3843..4769)
                     /gene="trxB"
                     /locus_tag="ACGE4T_002055"
     CDS             complement(3843..4769)
                     /gene="trxB"
                     /locus_tag="ACGE4T_002055"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFF8387298.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(4914..6347)
                     /locus_tag="ACGE4T_002056"
     CDS             complement(4914..6347)
                     /locus_tag="ACGE4T_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8387299.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(6347..7750)
                     /locus_tag="ACGE4T_002057"
     CDS             complement(6347..7750)
                     /locus_tag="ACGE4T_002057"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8387300.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         2128 a         1754 c         1336 g         2567 t
ORIGIN      
        1 gttttgaccg tcaaggaaat cttgcttttt tttaaaatta tcaaatgtta ttttatttaa
       61 atccttattg acaattttca catcttccga aaatctcgaa tctatgccct tcgatcgtgc
      121 atccacctag ctgttcttcg aaataattca tcggacacat atggatttct ttcgttgttc
      181 cgcacactgt acagataaaa tgatggtgat ggccgatttt cccctgacag caatgaaaac
      241 gaaacttcat ttctccattt aaatcggttt cttccagtaa atcgatctct gcaaaatcat
      301 ggagattccg gtaaatcgta tcataactta cccctttgaa ttcctgattc atatagtcaa
      361 aaacttcttt agcagatacg tagcggttac gcttcactaa ataagttaac aatgcttctc
      421 tttttttagt atatttaaaa ccgttatttt tcaatgtttc taatgattgt tcgatcaatg
      481 tctgtttttt catgacttat tcctccaaat caaaatgatt acgaagtacg cattctagag
      541 tatggtataa taaacataaa aaaaatgcaa gtgaggtttt atccatgtct tccgattcta
      601 cagaaatcgt tacttttttt gtcatctccg actctgctgg agaaactgcg acaaaactag
      661 cgcaagctac tatggctcag tatcctaccg ttgagttcaa cctattccgc agaacgttcg
      721 tgacagatga aaaaacatta aggcaggcat tgcaagatgc attagaagaa aaagcacttg
      781 tcctgcatac attaatcaat caagaactga tcgagataac acgttctttt tgtgaagaac
      841 atcaattatt tagttttgat gtgatgacgc caccgatatc agaaattgaa cgtttaactg
      901 gtatcaagcc aattcggcaa cctggtgccc tccatttatt gaatgagaac tatttcaaac
      961 ggatcaaagc aatggaattt gctgtaaaat acgatgatgg gaaagatcct cgtggttttt
     1021 tagaagcaga tgtcgttttg ctaggcgtat cccgaacttc caaaacgcct ttgagcttgt
     1081 ttttagctaa caaaaattta aaagttgcca atttaccttt gatcccacaa gcacatattc
     1141 ccaaacagct ttgggagatc gatcctaaaa aaatcgtggg actaacgaat aacccagata
     1201 ttttaaataa tatccgtaaa gaacggatgc gctcttacgg attaaaccct gatactgctt
     1261 attccgatat cgaaaaaatt agagctgaac ttgattttgc gaatgatctt tatgaaaaac
     1321 taggatgtgt agtgatcaat gtggcatctt tatctattga agaaacggct tcgcttattt
     1381 tgaatgcttt agacctagaa gatcatagtt attatggaac tgaaacaaca gaaaaaagcg
     1441 attaatcatt tttgtctttt tcttaaaaaa cactggagtg tgacaacgaa gaaatttcgt
     1501 tgtcacactc cagtgtttat ctatttgcac ctatcgttat tgatgttcca catgtttgag
     1561 tgtctggttc aaaatatcca gtacatgaga atcatcaaga ctatacaaaa tcgcttttcc
     1621 ctcgcgatga gacttgacga catgattttc tcttaataaa cgtagttgat gcgatacagc
     1681 agactgctcc atttccactg cttgggtgat tgctgtcaca ttttgttccc cgtctttcag
     1741 atatagcaga attttaagac gagtggggtc actcaacatt ttgaataatt gactgacttt
     1801 ctctactttt gatttttgcg tgtctggcaa tccattcacc tccaacttga aaaaagactg
     1861 acttctaaag tttaccactt tttccatcag tctcaagttt tttattcttc tgtcatcttt
     1921 ttgataatcg cttcataaga ggctactttt tcggctgcgt cttctgtaga gcttcctttt
     1981 acaccaatat aaaacttgat tttcggctca gttcctgaag ggcggatagc gatccagcca
     2041 ttttcttcca atgtatactt caatacgtcg gatggcggtg ttgttagttt ttctacgttt
     2101 ccttcacggt ctgttctcgt caattcttta aagtcttctg tttgaataac ttttgctccg
     2161 gcaaattctg tcggtgcttc tttacggaat ttttccatca aagcagagat tttcgcagaa
     2221 ccttcttgtc cgctcattgt cacagaaatc gttttttctg cgaaatagcc ataggtttca
     2281 aaaatcgttt gcaagccgtc atacaacgtt ttgccttgtt ttttatagta agctgctact
     2341 tctgctaata aaagcaatgc ttggatcgcg tctttatcgc gaacaaaagg tttaactaaa
     2401 taaccataac tttcttcgaa tccaaacata aaagtatgtg aatggtcttc ttcaaattgt
     2461 tggatttttt cagcaatgaa tttaaaccct gtcaacacat tgaacatagt agcaccgtag
     2521 tgttccgcaa ttgctgtagg caattcgctg gaaacgattg atttcaatac cgctgcgttt
     2581 tctggtaaag tgcctcttga ttgatgtgct tccaaaatat attgagcaaa caatgctccg
     2641 atttggttcc cagtcaatac ttcataagaa ccatctggca aacgaaccgc tgcgcctaaa
     2701 cggtctgcat caggatctgt tgcaatcagc aagtcagctc cctctttttc tccaagtcgt
     2761 atagcatatt caaaagctga atgttcttct ggatttggcg atttgaccgt agtgaaatct
     2821 ggatctgcca cagcttgttc gggaactagt acgaattgtt cgaatccagc ttgtttcaac
     2881 gcacgttcgc ccaacatttt ccctgttcca tgaagtggcg tgtacacaag tttcaaatct
     2941 ttccccattg tttcaaccaa ttcacgatca atcgtcactg ctttgatttc ttctagataa
     3001 gcagcgtcca cttcttctcc gatgatcgtg atcaacccgc tatgtttagc ttcttcgtca
     3061 gataatactt cgacttttag cggattgctg acttcacgga catatttcgt caatgcatct
     3121 gcgtctgcag gaggcatttg tccgccatct tctccgtaga ctttatatcc attgtaagcc
     3181 gctgggttgt gagatgcggt taccatgatt cccgcaaacg tcttcaaatg acgaactgca
     3241 aaagaaagct ctggtgttgg acgcagactt tcgaatacat aagacgggat attgtgatgt
     3301 gccagtgttt tggctgcttc cattgcaaat tctggtgact gatggcgtga gtcataagcg
     3361 atagcgacac ctcggcgttt tgtttcttca ccttgtgtat ccatgaaacg tgctagtcct
     3421 tctgttgctt gtcttaccgt atagatgttc attcgattga tcccaggtcc taagattccc
     3481 cgcatgcctg cagtaccgaa ttccaacggt gcataaaaag catcttcaag tttttcagga
     3541 tcttcaccca gatcatttaa ttgttttttt aagttttctt ctaaattttc ttcatgtacc
     3601 cattgttcat acacttgttc ccaagacatg aaaataacac ctctttctga aatatggttc
     3661 ttaataagta tagcattcac ttattagata tgaaagtatg aggaccaaaa aacgcttaaa
     3721 tcaagaaagg attcttttat caagcatgat tgcaagtatt cacataaatc ggattcagtg
     3781 gatttcacgc gaaaaaaaag agtaaaagaa caattgaaac cgttctttca ctctttttaa
     3841 acttatgctg ttgtttcttc taaagactcc aagtatttat acacttgttg tccagcaata
     3901 ccgccgtcac caacagctgt agtgatttgt cgcaaatctt tttcacgaac atctccgatg
     3961 gcaaaaacgc ctggcatatt cgttttcatt tcgttgtctg ttggaatcca gccagcttcg
     4021 ttcgtcaaac ctgcttctct aaaaggtgca gttaatggat ccagtccaac atagatgaat
     4081 actccgtctg ctgctaaatc aaattcattg ccggttttga cattacgtgc tttcacacct
     4141 gtcactacca tttcgtttcc aacgatttcg tcaacgactg tatcccacat aaaggaaatt
     4201 ttttcatttg caaatgcacg gtcttggatg atcttttgtg cacgtaattc atctctacgg
     4261 tggacgatca ctacttcaga agcaaattgt gtcaaataga tggcttcttc tactgctgag
     4321 tccccacccc cgatgactac tagacgtttg ttacggaaaa acgcaccatc acagactgca
     4381 cagtaagaaa cgcctcgtcc agcaaattct tcctcgccag gtacgcctaa tttacggtgg
     4441 atacagcctg ttgcgataat gactgttttt ccttcgtaac ttttatcttc acagatgact
     4501 tctttatggc tgccgtgatc cttgatatct tgaacgattc cataagcatt ttctgtccca
     4561 aatcttgaga cactttcata catcttataa gctagatcgg ggcccatgat cgaatcgaat
     4621 ccaggataat tttcgatttc tgccgtattg ttcatttggc cgccaggggc accttgttcg
     4681 atcatcaata cggataagtt tgaacgtgaa gcatacaaag cagcagtcat cccagcagga
     4741 cctgcgccaa taacgattac atcatacata tgtagattct cctttactta atcaagtaag
     4801 catactttac agccaatgcg aaaaaaagtc tattgttctg cttgcgtttc tcgaatagct
     4861 ttattatact cgaaaaatcc gctgaaaaac aatagacttt tataaatcat tgatcaattg
     4921 ttcgaaagat tttctccatt tgaatcaatt acttttttat accagttgaa tgattttttc
     4981 tttgaacggg ctaatgtgcc atttccttgg ttatcacgat cgacataaat gaatccgtac
     5041 cttttcttca tctcaccggt accagctgat actaaatcga tacatcccca agtcgtatag
     5101 cctaatagat cgactccgtc tagttctact gcatccttca tcgcctggat atgcgctgcc
     5161 aggtaatcga ttcggtaatc atcttccaca taaccatttt catctggcgt atcgactgca
     5221 cctaagccgt tttctacaat aaataatggt ttttgataac gatcataaat atcattcatc
     5281 gttattctca agcctaatgg atcaatttgc cacccccatt cacttgcttg cagatatgga
     5341 tttttgacag aagcaaagat atttcctgca gtctgttcca agagttcgtt gtcggtagtc
     5401 gaaactcgtg aagaataata agaaaaagag ataaaatcaa cagtatgttc tttcagcaat
     5461 gcggcatccc ctggttccat ttcgatctga atgccatttc gctccatttc tttcaatgca
     5521 taagcgggat attctcctcg tgactggaca tcaatgaaga aataattttc tcgatctgat
     5581 tttctagaag cccaaacatc ttcaggattg caagtgtatg gataatatgc tcctgctgcc
     5641 agcatacagc ctactttgtt ctccggatct acttcatgag caatcttcgt tgcgatggca
     5701 cttgcaacca actcatgatg tgccgcttga tatttgattt gttcttcatt ttctccttct
     5761 tcaaaataca gccctgcgcc cataaatggt gcatgaagga tcatattgat ctcattgaat
     5821 gtcagccaat atttgacaag tcctttatag cgattgaaga tgactcgaca cagattttca
     5881 taaaaaccaa cgagttctct ggaacgccac gcaccatatt ctttgatcaa atgcattgga
     5941 caatcaaaat gcgtaatcgt cacaagtggc tcaattccgt gcttttggca ttctttaaag
     6001 acatcctcat aaaatttcaa gccagcttca ttcggctctt tctcatcccc cattggaaaa
     6061 atacgactcc aggcgataga cattcgataa gtcttgaacc ccatttctgc aaacaaagca
     6121 atatcttctt tataacgatg atacatatcg attgcaattt ttgctggata aaaatgatcc
     6181 tcatcaaatt caaacatttt tttctttccg gtgataacag gaaaacggtc ttttccgatt
     6241 ggcacaacat ctacgtttgc tagtccgcgt ccaccttcat cataagcgcc ctcacattga
     6301 ttggctgctg tagcaccgcc ccataaaaaa tcttttctga accccattat acttcctcca
     6361 ttatatgtgt attacctgat aattctcttg ccagttcatc ttgaacttcg attccttctt
     6421 cttttaatcg gctgattgtt tccttgaatt gcggtaaata ctgctggtgt gcaataaaca
     6481 actcatccat gatccgtttt gctgtctttc ctgatcgtat aagtggattg atcgtaaacg
     6541 cctgtaaagc tgtcccataa tcaccagtca cagctgcttc gatcgtcagc aattccatat
     6601 tcttcattac ttgcaaccag cctctttcag cagcaggaag agaaccaaac gctacatttc
     6661 tcgctccttg tgcgccaaca taggcagtga cttctacaac acaatctgcc gacaggtcag
     6721 gaacagctcc atcattcttt gtagatacaa cgatctgtgt ttctttattt ccatagattg
     6781 aagcaatcgt ttcacaggca gcgtctgaat aataagcgcc acctcgtttt tgcaattgtt
     6841 caggtttatg gtctaattct ggatctttgt ataattcaaa tagctctgct tctgtttctt
     6901 tcacttgctg cgctcttgta ccaatcgttt gatattcttc taaagcatgt tccagcattt
     6961 cttcttcacg atagtaatag cggtggtagc cacaagggat catgttcatt tgttttaatt
     7021 gttctttgaa aaacgggata tcaaagatat ttttcggtaa tccattatct tcatcgtata
     7081 aacgatcaat caattctgcc gtgcggtcat ttcctttatt atctgctact ttgtgccaat
     7141 ggaaatgatt tagaccggca aacttgtaga tcagctcttc ttttttcttg ccaagcattg
     7201 ctggttctgt catcatcgcc atcactggca cattgcataa tccaatcact ttatcccatt
     7261 tgccatatct gataactgct tctgtcacca tcccacttgg gttcgcgaaa ttgatcagcc
     7321 aagcatttgg acataaacgc ttcatgtcct caacgattgc caaaacgact ggaacggtac
     7381 gaaaagcttt gaatatcccg ccagcaccat ttgtttcttg cccaatcatc ccataataac
     7441 tagggatccg ctcgtctttg attcgtgcat ctaattgacc gacacgaaac tgagtcgtaa
     7501 caaaatccgc atctttcaat gcttcttccc gatccagagt tgcatagatc ttgacttcat
     7561 atggagaagc atcccacata cgctgagaca tctgactaac gatctcaagc ttttcttttc
     7621 cttcttctac atctacgagc cagatttctc gtatcggtaa ttcctcatat cttttgataa
     7681 atccttccat caactcaggg gtgtaactgc tgcctccacc aatcgtagcg attttgattc
     7741 cttttgccat gttctcactc ctttactttt ttacactttc attat
//
LOCUS       DAXWLG010000119         7767 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXWLG010000119 DAXWLG010000000
VERSION     DAXWLG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.120"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(78..150)
                     /locus_tag="ACGE4T_002058"
     tRNA            complement(78..150)
                     /locus_tag="ACGE4T_002058"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="ACGE4T_002059"
     CDS             531..1100
                     /locus_tag="ACGE4T_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFF8387301.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="ACGE4T_002060"
     CDS             1087..1947
                     /locus_tag="ACGE4T_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8387302.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE4T_002061"
     CDS             2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE4T_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFF8387303.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="ACGE4T_002062"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="ACGE4T_002062"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFF8387304.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE4T_002063"
     CDS             4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE4T_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFF8387305.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="ACGE4T_002064"
     CDS             5995..6864
                     /locus_tag="ACGE4T_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFF8387306.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="ACGE4T_002065"
     CDS             6919..7728
                     /locus_tag="ACGE4T_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8387307.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
BASE COUNT         2652 a         1316 c         1696 g         2103 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaa
//
LOCUS       DAXWLG010000120         7750 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXWLG010000120 DAXWLG010000000
VERSION     DAXWLG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.121"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            48..749
                     /locus_tag="ACGE4T_002066"
     CDS             48..749
                     /locus_tag="ACGE4T_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8387308.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1321..2337)
                     /gene="tsaD"
                     /locus_tag="ACGE4T_002067"
     CDS             complement(1321..2337)
                     /gene="tsaD"
                     /locus_tag="ACGE4T_002067"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFF8387309.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2334..2798)
                     /gene="rimI"
                     /locus_tag="ACGE4T_002068"
     CDS             complement(2334..2798)
                     /gene="rimI"
                     /locus_tag="ACGE4T_002068"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8387310.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2805..3347)
                     /gene="rimI"
                     /locus_tag="ACGE4T_002069"
     CDS             complement(2805..3347)
                     /gene="rimI"
                     /locus_tag="ACGE4T_002069"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8387311.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3331..4053)
                     /gene="tsaB"
                     /locus_tag="ACGE4T_002070"
     CDS             complement(3331..4053)
                     /gene="tsaB"
                     /locus_tag="ACGE4T_002070"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFF8387312.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4244..5224)
                     /locus_tag="ACGE4T_002071"
     CDS             complement(4244..5224)
                     /locus_tag="ACGE4T_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8387313.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5248..6732)
                     /gene="galT"
                     /locus_tag="ACGE4T_002072"
     CDS             complement(5248..6732)
                     /gene="galT"
                     /locus_tag="ACGE4T_002072"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFF8387314.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6744..7733)
                     /gene="galE"
                     /locus_tag="ACGE4T_002073"
     CDS             complement(6744..7733)
                     /gene="galE"
                     /locus_tag="ACGE4T_002073"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFF8387315.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2158 a         1686 c         1376 g         2530 t
ORIGIN      
        1 aaaaaagtga caataaatat agtgaacaat aaggagagga ctttttaatg cgtgttaata
       61 aaatcgattt tcacttttca aaactcagat atcaacctga ttttgaacat ttctcggatg
      121 aagagttcca acagatcaag gaacatactg ttcttcgttc ttataaaaaa ggacagatcc
      181 ttttcgacga tggtgatccc agacaacggt tgtatatctt gaccaaagga ctcgttcgtt
      241 tagaacgtta tgatgaaagt gcaacttatt actactatga ctatgtgaat acaaataacc
      301 tttttcctat gtcaggaatg tttggagaag agacctacac ttatacagct caagcgatga
      361 ctgatattga aacattctac cttcctgttt cctactatga aaaattagca cgaaaaaatc
      421 ccgaacagtt gatctatatg atacaaaaaa tttctaaagt agttgaaata catgaattgc
      481 gtatccaaat cggattgacc tctagcgcct ttgaccgggt aaaacaaaat ttattcattt
      541 tgaaagaaga attgggcgta ccaacaaaag atggtcatat caccattcct tatccgatca
      601 ccttaaaaga attagcagtg aatagcggca cgacacgtga aactgctgga caagtaatca
      661 aacaactacg tgattccgga ctgttagatt atgacaaaaa gcgttttact ttctgtaaga
      721 cagctactac tccagctatg agagaataac gcgaacaaaa ataagcagtg accctgatat
      781 ttatcagggt cactgcttat tttcctccct aaaacttctg aaagatccgg cagttttaga
      841 tcactgggca agactcaaag attttctatg tgtgtttcag ttatgtaata ttcactgttt
      901 gcaaaaaata ctgatagatt aactagagga agcgtcttgt cctcatccga cctagattat
      961 tcgatattcc tcgacaataa acgattaatc agtgagggca atcagaggaa cggataatga
     1021 tgaataagct gtaaaaaata gggctttgac aaaagcgtgt cacagccctt atttcgatta
     1081 aacggtgtgt acctgtcaac tcttcggtca caagtcataa tgttcataaa catgaggagc
     1141 tgttttttga aattgttcct tgtgaatcag agttaaacga caggcttacg aactcttgat
     1201 taaacggcgt tcaaaagctt gcatacgcgg taataagaac agccaggtca aaaacatgag
     1261 aagccgtttt tcctgggctg ttcttattac ttgatgcagg gcagattttt cacaacctct
     1321 ttattcagat aaaaccaagc ttggatcagc attcaactga tagctagcaa aatttctttt
     1381 ttgcatttcc acatgtgctg ctgctccaat cattgcagca ttatctccac aaagtcgaag
     1441 agggggaatc actagttcta cctcagggag tttagttgcc aaagcatcct gcaatccttc
     1501 acgcaaccct tgattggccg ctacacctcc agctacaatc agttgcttga ctggataatt
     1561 ttgacaagct cgaagtgttt tagtaatcaa tacatcgatc acacttgctt gaaagctagc
     1621 tgctaaatca tttttgtcta gatcttctcc acgctgttgt gcattatgca ccaaattgat
     1681 aaatgcactt tttaatccgc taaaactgaa atcataatta tcttcatgga tcatcgctcg
     1741 tggaaaatgg taattatctt ttccctgatg agctagctga tcgatttctt ttccgcttgg
     1801 gtaagaaaga cctaatactc ttccaacttt gtcataagct tctcctgctg cgtcatctct
     1861 tgtctcccca atgatttcat atgatccatc ttcttgcata tagactaatt ctgtatgtcc
     1921 tccgctaaca agaagtgcca tcaatggaaa ctggaatggt ttaaccagac gggctgcata
     1981 gatatgccct gccatatggt ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc
     2041 cttagctgca gaaataccaa tcagcaacga gccgacaagt cctggtccat aagtgacagc
     2101 gactgccgat aaatcttctg ccgacacacc agcttctacc agtgcatctt ccaagcataa
     2161 ggtgatttgt tctacgtgat ggcggctagc tacttcagga actacgccac caaaacgttt
     2221 atgactgttt atttgagaag caacgatgtt agataagatc tctgttccat ttctaacgac
     2281 cgctacactc gtttcatcac aacttgattc aatcgccaga atcaattctt cattcatttc
     2341 atcgtctccg tttttaactt tctgctcata atgatcgcat cttccgtcgg attgcgataa
     2401 tatgatttcc gtttacctaa tatacgaaac ttttctgatt cataaagttt tcttgctgct
     2461 tcattagata gacgtacttc taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc
     2521 agtaattgta acaaatgccg tgcatgccct tttccttgtt ctgaaactgc aacagcaata
     2581 tttgtgatct cgacttcatc tattactttc gagtatccaa taaagccaca gatctggtta
     2641 tcttcaacta aaagcagata atcagagtga ggctgttgga tgtctgccaa aaactgcatt
     2701 ttcgtccaag gtgaaccatg tggataagca ttttcgctaa tcatccatac gcattctgcc
     2761 aagtacgctt catcaaaatc tttttttctg caaatcaact aatctcactc attttctaaa
     2821 agttttatca tatccaacgc atcttctttt gtttcagtgt aatatctttt tttgatcgtt
     2881 gttgcttgaa aacctaactt ccgataaagt ctttgtgcat cttgattact catacgcact
     2941 tctaatgaca tcgtctcaca gcgattcatt atagcaaagt tctcgatttc gtcaataagc
     3001 agcgaaccga ttcgtttacg ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa
     3061 tctaaaccga acgctcttga accgataaaa ccaatcattc ttccattatc aaagatacct
     3121 agatatagat gaggaagcgg tgacttcaat tctgatataa aggcactttt tgtccatggc
     3181 aattcgccat tatagacttc tcgttcgaca tccagcagct ctttgatatc ttctgtagtc
     3241 aattctctta gtacgtaacg acgatcgatt tgttcgattg ttttttctgt ataaatcatt
     3301 ttatcttgaa aaaagcttct ggcatacgct ctaaattttt tcaacataac tttcgtctcc
     3361 aggtgtgtgc gtctccaacc atttttcttc tgcttctacc cgttttaaat agcgaggaag
     3421 aaaagcttgt ttctctgcaa ctggttcagc taaagcacct aattgagcca cagttacacc
     3481 acttggaata tcccatgctg gaactgtatt aatcatcgca ctaggcaata tttcctttat
     3541 ttcatagaag aatttgtgac aatcactccc gacgaaataa atagcttcat tcagcttttg
     3601 tagttgtgtg aacaaatcag aagcagaaat atgtacatct tcaagtacaa tttctaatct
     3661 attttctttc cagcggtatg ccccagcgta tacatttttt cttcgagcat caaacatggg
     3721 cacaataatt ttatccacac cgatgcaatt agccgcgatg gttttcagac tagatactcc
     3781 tactagctct ttatctaatg tataagcaat cgtttttgct gtcgttacac ctatacgaag
     3841 ccctgtataa gaaccgggac catcagaaac cgcgattcgg tcaatgtctt ttggacttat
     3901 ttgaagatct gcaaacaatt ggtcgattgc aggcatcagt gttacactgt ggttgcgttt
     3961 gatatttgtt tgtatttgtc caagaatttg ttcgtcttct accacaccga tcgccaaagt
     4021 ttggtttgca gtatctattc ctaatgtaat catatatttg atcctttctg ctatgacatt
     4081 ctcgccttcc attgtagcat acttttttat tctgaggatg aaaataccaa aggcaaaact
     4141 agtacaatga tacatgatta tctgctggcg ggcaaatttt gccccttcct gaaacacaaa
     4201 aaagctgcgg tatcacttca tttccccaca gcttttccat cttttatttc gttgattctc
     4261 gaataatcaa ctctgtttcc acaataatct ttcttgaaac cggtgctttg gaattactta
     4321 attgaagaat cgtctccact gctagctctc ccatccattc ggtataaact tttacagtag
     4381 aaagagaggg cgttacatat ttcgctacac tgacatcatt gaaacccatc acagaaatat
     4441 cttctggtac cactaaacct ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat
     4501 cattagctgc aaaaagcgca tcaggaacat gcggattcgt ttttaaatac tgcttcactg
     4561 cttgatatcc tgactcgaca gaaaaatctg cttcgataat tggaccttcg tcgtagcctt
     4621 tttctgccaa aacttgttga aatgccataa ttctgggatc ttttagccgc tgatggtttt
     4681 tcttcgtata ttctgttcca gcaagcatag cgatatgttg cgctttatgg gagacaaaat
     4741 gttcaatgac tttttgaaca cttgacttaa agtcgacaac tagagagtcg attccttgag
     4801 aagtgccgtc agaatctacc aaaagcaagg cattttggga tcctttcatt tctaataact
     4861 gagcttcgtc gaattttcct agtgcgatcg ttccatctgt cggaagatca ctaatggaat
     4921 cccacgtttc tttcaataaa gtaacgtttg cttcttctgc ctttttttcg attcctagac
     4981 gaatagataa ataataaaga tcttcaagct cttccgtatc gtcgtaccac tggatcaggc
     5041 gaagcgtttg cttagtattt gctgcatttt tacgatgttt cgtataattt aagcgttctg
     5101 ctgcttcaaa aattttttgc tttgtggcta agccaacgga aagatcagga tcatagttga
     5161 gaactcttga aacagtagca ggagaaactc ctgccaactg cgcaatgtct ttaattgttg
     5221 ccatttgtct ctcctccttt tttacgttta taaaattgca gtaaatcgtt caaacgctgc
     5281 ctgtcctttt tcatctcgct tgaatactcc agcgtcttcc agtacacgtg caaaaacctg
     5341 acctacagat ttttgtacga catcggtgac cgtatctttc gtcaaatcag gatgttcttt
     5401 ttttaattgt tctgcccacg gacgatgata ttcagccatc tggttttctt ctcctaataa
     5461 gaaatgctct acttctacta gttctggttt caaacgaggt ggtaaaacag caagccccat
     5521 tacttcgatc aacccaatgt tctccttttt gatatgttgg acatctttat gtggatgaaa
     5581 aatcccatca ggatgttctt ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa
     5641 gtaattgtcc cttcttcgtg caattggcgt aatcgtatgg tgtggtgtac catctgctga
     5701 aaaagctctg atagatactg tttcatccga atatgctttc cacttttcca atactgagtt
     5761 agctgcttca atcaacaatt ttttctctgt tgattgcaaa cggatcacag acatcggcca
     5821 tttcacgact cctgcgatga cttgcggata atctgctagt ttgataagtt tttctatcgg
     5881 tgcttttgcc ataggaaatt catgacgtcc tgcctgataa tgatcatgag aaagaatcga
     5941 tcctccaaca atcggcaagt cagcattcga accaacaaag taatgaggca agacttcagt
     6001 aatccgaagc agtctagcaa atgtttcctt tgtgattttc atcgggcggt gttgttctga
     6061 taaaatgatc gaatgttcat tgtaataagc ataaggagaa tattggaatc cccagctttc
     6121 accgtctaga ttcattcgaa taatccggtg attggtgcgg gctggatagt tcaagcgacc
     6181 tctatatcct tcgttttcca tgcaaagcat gcaggtagga tagtctactt ttaccgcctc
     6241 gcgttctgct gcgatttgtt ttggatcttt ttctggctta gataaattga ttgtgatttc
     6301 cagttcacca tactccgact ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt
     6361 gatgtagtca tttttcttac ttaaattaaa gaaatactca gttgcttcct ctgggctttt
     6421 cgaatagtgc tgtgcaaaga atgcattgac gactgaaggt ggcggtgtga gaaaatccat
     6481 taattgcgct tcaaaaatct cttgttctgc tggcaattct gaaagaacac cattttctct
     6541 tgccttagcg acaagctgat ccaataagtc tacagattct tccttaactg gtcttacttc
     6601 aatgctttct agagcatctt ctcctaccat ccctaaaaga cgattctgta aatataagcg
     6661 gtccatttcc atccatccgc cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg
     6721 actgattgac atatctcatt cccctattct tcataaccat ttggatggct gacatgccaa
     6781 tcccatgctg ttttgataat tgctttgata tctgtgtatt caggtttcca acccagaacc
     6841 tctctcgctt tttcacttga agcgactaat cggctaggat ctccagctct tcttggtgct
     6901 atttctgctg gaatttcttt tcctgtcact tctctagctg cttctagcat ttccttgact
     6961 gagtatcctt tgctgctgcc taaattgaag aagttgcttt catttccttc tttcaagtat
     7021 tccaaagcta aaatatgtgc agcaatcaaa tcttctactt gaacataatc tctgatacat
     7081 gttccatctg gtgtatcata atcgtctcca taaacagcca atgcttttct ttgaccaaga
     7141 gctacttgta aaatgattgg gacaagatgc gtttctggtg tatgatcttc tccgatagat
     7201 gcatctgctt ttgctccggc tacattgaag tagcgcaaag cgacatagcg catgccatat
     7261 gcttggtcac accacttcat cattttttcc atcatcaatt tactttcacc atacggattt
     7321 ttaggattag ctggtgtatc ttctgtgatt ggagactctt ttggttctcc gtaagttgct
     7381 gcagtagaag agaacacgat tttattcaca ttgaactcgt gcatgacttc aagtaagatt
     7441 tgcataccgt atacgttgtt gttgaaatac atcaatggtt tttcgacaga ttctcctact
     7501 aaagagcttg cagcaaaatg gattactcct tcaatcggtt ccttttcaaa tacggaacgt
     7561 agaaattctt tatcccggat gtctccttca taaaaatgag catctggatg tactgcttgt
     7621 ttgtgacctg tgagcaaatt atctaccaca gctacttgat accccttttg aactagctgg
     7681 tctacagcgt gcgaaccgat ataaccggct ccgcctagta ctaaaattgt cataaaaaac
     7741 actcctttga
//
LOCUS       DAXWLG010000121         7594 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXWLG010000121 DAXWLG010000000
VERSION     DAXWLG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.122"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACGE4T_002074"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACGE4T_002074"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFF8387316.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACGE4T_002075"
     CDS             complement(2032..3285)
                     /locus_tag="ACGE4T_002075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFF8387317.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACGE4T_002076"
     CDS             complement(3291..4214)
                     /locus_tag="ACGE4T_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFF8387318.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACGE4T_002077"
     CDS             complement(4435..6927)
                     /locus_tag="ACGE4T_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFF8387319.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACGE4T_002078"
     CDS             complement(6934..7401)
                     /locus_tag="ACGE4T_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFF8387320.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2092 a         1658 c         1219 g         2625 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tcca
//
LOCUS       DAXWLG010000122         7586 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXWLG010000122 DAXWLG010000000
VERSION     DAXWLG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.123"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(124..414)
                     /locus_tag="ACGE4T_002079"
     CDS             complement(124..414)
                     /locus_tag="ACGE4T_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387321.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(532..792)
                     /locus_tag="ACGE4T_002080"
     CDS             complement(532..792)
                     /locus_tag="ACGE4T_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387322.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(929..1342)
                     /locus_tag="ACGE4T_002081"
     CDS             complement(929..1342)
                     /locus_tag="ACGE4T_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFF8387323.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1798..2283)
                     /locus_tag="ACGE4T_002082"
     CDS             complement(1798..2283)
                     /locus_tag="ACGE4T_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387324.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2422..2637)
                     /locus_tag="ACGE4T_002083"
     CDS             complement(2422..2637)
                     /locus_tag="ACGE4T_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFF8387325.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2861..3661
                     /locus_tag="ACGE4T_002084"
     CDS             2861..3661
                     /locus_tag="ACGE4T_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8387326.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3680..5548
                     /locus_tag="ACGE4T_002085"
     CDS             3680..5548
                     /locus_tag="ACGE4T_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8387327.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5541..6032
                     /locus_tag="ACGE4T_002086"
     CDS             5541..6032
                     /locus_tag="ACGE4T_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFF8387328.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6020..6574
                     /locus_tag="ACGE4T_002087"
     CDS             6020..6574
                     /locus_tag="ACGE4T_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFF8387329.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6592..>7586
                     /locus_tag="ACGE4T_002088"
     CDS             6592..>7586
                     /locus_tag="ACGE4T_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFF8387330.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2703 a         1212 c         1441 g         2230 t
ORIGIN      
        1 taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc aaaaaagata
       61 acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat acattttaat
      121 acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag agatcataag
      181 ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc atatttcttc
      241 aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc agcaccattt
      301 ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa gtaaaaacaa
      361 agctttactc ataactaaca aaataccagc tccttttatt tttttctccc ccacattcaa
      421 agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat gaataacagc
      481 tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag tttatttttt
      541 tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc cgatttcgta
      601 gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat ggataaaata
      661 gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata agtaaaagaa
      721 agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga caatcaaatg
      781 atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa aaacaaaata
      841 tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat ataaaaatcg
      901 cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt gtttcttttg
      961 gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag gttccagtga
     1021 ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc gaatgtcttt
     1081 ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga ttggcggaga
     1141 tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag ccaggcatat
     1201 cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga taacttgaaa
     1261 cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg aacatgtcgc
     1321 gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt ttatttatcc
     1381 ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag ctttgtgaat
     1441 atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat cattaaaata
     1501 ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa tatagaaaaa
     1561 ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg aaaatgaagt
     1621 agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat aagagaaata
     1681 gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata actttctcag
     1741 aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat catctaatca
     1801 aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca ctcgataaaa
     1861 accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg tgaacaaata
     1921 atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta attcggaacg
     1981 gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag aaggtacagt
     2041 gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg attggctatg
     2101 ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag tacttttagt
     2161 tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat tctttgtttt
     2221 atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa tccctttatg
     2281 catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa acaaagaaaa
     2341 gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga agataataac
     2401 ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa tcattgggaa
     2461 aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct tcgcaaaaat
     2521 agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc ctaccaacag
     2581 ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaaag ttttcattag
     2641 aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca ttgcttatat
     2701 aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg ctcttccatt
     2761 agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt agtgagcttt
     2821 tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt ggttggatat
     2881 tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa gtatcgtcga
     2941 aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat gccgtataca
     3001 gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca tagaagaagg
     3061 gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata atgcaggaat
     3121 aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg aattatcaga
     3181 acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga tgagccaagc
     3241 agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt cttctgaatc
     3301 aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag cgatgaacag
     3361 ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg taggtgttgc
     3421 accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag ctctttctta
     3481 tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa gtacgatccc
     3541 tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt atctttcaga
     3601 aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa caagaggata
     3661 aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat tgaaattact
     3721 agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa ccagtaatca
     3781 aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg caaaaatcat
     3841 ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg atgagatcat
     3901 gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa tcgcttattt
     3961 actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt ctgaagaagt
     4021 cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga tgatcaatga
     4081 ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag gagatgagct
     4141 agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga cgaaaacaga
     4201 acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga atgctagtaa
     4261 acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa tctctagtaa
     4321 ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata tccaagaatt
     4381 caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc aatatgaaaa
     4441 agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga taaggaaagc
     4501 taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac gattagatct
     4561 cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct ctttattgat
     4621 aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga tagaaaaaaa
     4681 gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag ctatcttgca
     4741 acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt tagcatttcg
     4801 atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga caggaaaagg
     4861 aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata tacaaataac
     4921 acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg ctgtttttac
     4981 aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga ctaatctttt
     5041 tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat tagccgggtt
     5101 tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc aagaatattt
     5161 aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat ttgaaaaaag
     5221 gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg cttttcctca
     5281 cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa ctgatcaagg
     5341 tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg aagaaatgac
     5401 ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac tcataaaaat
     5461 gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa aactaatcaa
     5521 aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc gcagcttcag
     5581 cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat caaacatata
     5641 gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa atccgttcag
     5701 gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc agaaaaatgc
     5761 aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt gaagatatcc
     5821 attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca caagcagctt
     5881 tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa agtgaacaac
     5941 cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt acgatcaaag
     6001 aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat ttatgaggct
     6061 gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac cagttgtctt
     6121 gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc gagtaacgaa
     6181 agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac cgttcttatc
     6241 tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc cagaatacta
     6301 caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg gtgtattccc
     6361 ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga tcgaaacctt
     6421 agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc tgatgaactt
     6481 tattggtggt tggataactg attttactac tggatttgta tgcagacagc aaggaatcgt
     6541 tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta aatgtatggt
     6601 gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat taggccgaca
     6661 aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat gatcgtagat
     6721 aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc aatacctgat
     6781 agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg aatttatcct
     6841 aaaaaaggga taccaaccat caatatcgtt tccacaggaa aaagtggccc attagcacaa
     6901 tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca gccactaatg
     6961 gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa gtacaaatat
     7021 gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat agcaaaaatt
     7081 ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga agcagtagat
     7141 acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat tggtgtgata
     7201 caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc aggaaatgca
     7261 gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc tttgcttggt
     7321 ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat tgggaaagga
     7381 aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca aaatgcatgt
     7441 gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt agaagtaggt
     7501 gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat cattgcttgg
     7561 gtagcaagtt ttggattata tagctg
//
LOCUS       DAXWLG010000123         7347 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXWLG010000123 DAXWLG010000000
VERSION     DAXWLG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.124"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            130..2739
                     /gene="clpB"
                     /locus_tag="ACGE4T_002089"
     CDS             130..2739
                     /gene="clpB"
                     /locus_tag="ACGE4T_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFF8387331.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2890..3576)
                     /locus_tag="ACGE4T_002090"
     CDS             complement(2890..3576)
                     /locus_tag="ACGE4T_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387332.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3554..4048)
                     /locus_tag="ACGE4T_002091"
     CDS             complement(3554..4048)
                     /locus_tag="ACGE4T_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8387333.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4068..5288)
                     /locus_tag="ACGE4T_002092"
     CDS             complement(4068..5288)
                     /locus_tag="ACGE4T_002092"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8387334.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5435..7270
                     /gene="lepA"
                     /locus_tag="ACGE4T_002093"
     CDS             5435..7270
                     /gene="lepA"
                     /locus_tag="ACGE4T_002093"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFF8387335.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2423 a         1400 c         1507 g         2017 t
ORIGIN      
        1 tataagtcga gaaaaaaagt caaaaaacta tgtgtatatt ttgaccaaaa ttgaccaaag
       61 cgatataata gtttttgtaa agatcaaaga aataagtgat cttaccttta attaggaggg
      121 tatacctgta tgaatattga gaaaatgact acgacgcttc aagaagcgat tgctgaagct
      181 caacagattg cggtcactcg aaaacatcaa gatatcgaca ttgcccatgt atggaaaatc
      241 tttttacaat ctaatcattt tggtcgaaat ttctatacgg atgccggatt ggatgtcgaa
      301 tcttttgaac atgaaattga tcgtctactg gatgagtatc cagttgtttc tggtggaaac
      361 gtccaatatg gacaaaacct cagccagaat ttctttcgat tattgaatga agcagaccaa
      421 atacgtgaaa gcttcggcga cgagtttctt tcaacggaag tcgttatttt agcgttgatg
      481 aaattaaaaa attatccgtt aactgtttat ttgaataaaa acggattatc tgaaaaagaa
      541 ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag tgacctcaca aaaccaagaa
      601 gaacaataca aagcattaga aaaatacggc gttgatttag tacagcaagt caaaagcgga
      661 aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg atgtcatccg tattctttca
      721 cgaaaaacaa aaaataatcc tgttctgata ggagaacccg gtgtagggaa aacggcaatc
      781 gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc ctgaaaattt aaaagataaa
      841 acgatttttt cattagatat gggtgcattg atcgctggag caaaattccg tggtgaattt
      901 gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa gtgatggacg tatcctcttg
      961 ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa cagaaggaag tatggatgcc
     1021 ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac atttgatcgg tgccacaact
     1081 ttagatgagt accgtcaata tatggaaaaa gacaaagcgt tggaacgtcg ctttcaaaag
     1141 gtattagtca aagaaccaac agtggaagat acgatcagta ttctgcgtgg attaaaagaa
     1201 cgttttgaga tccaccatgg tgttaacatc catgacaacg cattagtagc agcagctact
     1261 ttatctgacc gttatattac tgatcggttc ttaccggaca aagcaatcga tttgatcgat
     1321 gaagccagtg caacgattcg ggtagaaatg aactctatgc caacggaatt agatcaagtt
     1381 actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac tgaaaaaaga atcagatgat
     1441 gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag ctgaacttcg tgaagaagca
     1501 aatgcgatga agatgcagtg ggaaacagaa aaagaagaag tcaattctgt ttcagcaaaa
     1561 cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg cagaaaataa ctatgattta
     1621 gaacgcgctg ctgtattgcg tcatggtacg atacctcaat tagaaaaaga attgaaggaa
     1681 ttagaagcta aagcaaaaga cagcgagatt aagatggttc aagagtccgt taccgaaaat
     1741 gagattgctc aagtcgttgg cagattaacg ggtatccccg taacgaaact tgtagaaggt
     1801 gaacgagaaa aactaattaa attgaatgaa acacttcata aacgagtgat tggtcaagat
     1861 gaagcagttg atgcggtcag tgacgcggta attcgttcac gggctggatt gcaagaccca
     1921 aatcgtcctc taggttcttt ccttttccta ggaccaactg gtgtcggaaa aaccgaatta
     1981 gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc atatggtacg gatcgatatg
     2041 agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg gagctcctcc aggctatgta
     2101 ggttacgaag aaggtggtca attaacggaa gctgtcagac gaaatcctta tacaatcgtc
     2161 ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca atatcttgtt acaagtccta
     2221 gatgacggac gcttgactga ttcaaaaggg cgagtagtag atttcaaaaa tacggttctg
     2281 atcatgacca gcaatatcgg ttcacagtta ctgctggaag gcgtgactgc tgatggaacg
     2341 attccagaag cagtggctga acaagtcaac acgctattac gtggaaactt caaaccagaa
     2401 ttcttgaacc gaatcgacga cacgatttta tttacaccat tgagcctaga caacgtgaaa
     2461 ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc tggaacatca agaaatcctg
     2521 ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa atgcttatga accagcatat
     2581 ggtgcaagac cattgaaacg atttatcact aaggaagtag aaacaccact agctaaagaa
     2641 atcgttgcag gacatgttat gcctaaatca aaagtaacga ttactttact tgatggacaa
     2701 cttcatttca aaacagaaga gctagaagaa atcgtctaat cttccattga gactataaaa
     2761 gaaaaaaggg gtctgacaca actagtgtca gacctccttt tttcaaataa acagtgttcc
     2821 agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa gctgcctgct tctgtcacaa
     2881 cctcttttat taggtatttt tcactttttc taatttataa ctttcaaagt aacgctcatc
     2941 ttttttactt tgagaccatg caaacaatcc cattaaggca gcacctatcg tatttgtcag
     3001 caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca aacagtgtgc catcagaagc
     3061 agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat aagaattccc agaactccca
     3121 aaacacgccg caaagtccag caaacgcaaa gccgaataag aggaacaacc aaggactggt
     3181 ttttgatatc tcagcatctt tcatcaagta accaatcaat ccatagccaa gagctgtcag
     3241 gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa gagatgatac tgatcaaatg
     3301 catccctgtg cctaaaaaga cagagatgaa caagaaaaac cagtaaaaca aaacgattgc
     3361 tgctggtatt ttaagtttga aaactttcaa aatgacttgt ggcatgaaaa tcaatgcaat
     3421 ccctaataaa gattccacga taaatgtgat tccttgagaa gtcgaatatt tttgtgtgat
     3481 aaattcataa aaattatagc agacaacgaa tacaccaaaa gcaagtaaag ctatttgcac
     3541 ctttttgatt tctttaatca ctgaaagtcc ctccatctaa aaatcttttt acttctgaaa
     3601 gcaactcaat ttctgaagaa accacttttc cgttcaattt gatcaaacct attgtatata
     3661 agttcaggta gtgaaattga ttttcagctg tttcttgtag tgcagcgatt tttttcgggt
     3721 gggttccgcc ttgctgtcga gtatcagtat ataagccgat cactgggatg cctttcgcat
     3781 aagccacacc gatttccgat gctaccccgg catcgatcgt cagcccatct agtaaagcaa
     3841 tcattaaatc actagccaac actttttctg tgtcagcaag agcaatcatt cgactgtctg
     3901 cataagcagt tttgtcattg attgcttcat tttcttgagg aagatagatc gaaagctcag
     3961 aagagagctc tctgatttct ttaactaact gttggttata taacaaatca cttttagcaa
     4021 acattggtgc ggcaaaataa attttcatct aaacatcatc ctttttatca aaaataatcg
     4081 ctaaagtaag cttccatgtt tggaatgata cgctctagtg acttcagtgt ttcttcatct
     4141 gtttctaacc gttcgattct tgccaaataa gaagcacggc gataattcat catcaagcaa
     4201 gtcaatgttt ggatatccag tcgaacggat tttcctatag gttcatttaa gacacggact
     4261 tgtccctgtt catcccatgt taaagcgaag acaccattat tccaaggagc gacaggatct
     4321 tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg gaaaccgttg aaggaattct
     4381 tttacatcaa cgatccgagc cataaaatat ggttcgatct gctctttgat ctgactatct
     4441 tcaatcaaaa atgctaaagg ctcattttta taaatatctc catgtaccca gtagatcatg
     4501 gaaaaatgtg cggaaataaa gttccataag ccatttcgag cttcttgatc aagatagaac
     4561 atttctttta cgtgaaatat ttcttcagct acccagtaga acagaacacc tcttggttca
     4621 tggttgctat cgtagtagac agcggctgtg cgctcatctt cattttcgaa acgccaatac
     4681 tcttcccaat gaaaatcact gcggaataaa gcaccatggt tttgttgggc aaatttgtca
     4741 taaacggaaa atacgtctgg atgatcaact ggtaatcgct caaccatacc acttactggg
     4801 atttgtttag gtagctgtgt atctctgatt ttaaaggaaa gtttatcaga catgatttcc
     4861 catcctttac gccgatagta ggggatactg taaggataca aataagagat ccattgctta
     4921 ttcttgcgca tattatccaa tgcaacgata attagatctt gcatcaatcc atggttagca
     4981 tattctggat aagttccaac tccagtcaca ccacccattt tataacgtgc accgtgaata
     5041 ttgacttcac aaggatagat agcgatttga gagatcagtt tattttcgtg gaaccatccg
     5101 aacactttcg atacttccaa aatcggtttt ttggaacgga taaaagctcg tttattctca
     5161 aacccgcttt cctcgatgtc tgcttcagtc acttggaaca cataggaaag caattcattg
     5221 aattgatcga tatgttcttg agtgaccggt tttaattcaa gattttctct aaaatctttt
     5281 tgatccattt tttcatctcc atttattagc ttgataacag tatagcaaaa ttctacaaaa
     5341 aaaacttcta tagctgacta attaaaaact ctttaaatta ctgcttttcg ttggtataat
     5401 ggaaagtgct gattatatga aaagaggaga cgctatggat ataaagaaaa tgaaagaacg
     5461 ccaagagcag attcgtaatt tttcgatcat cgctcatatc gaccatggga aatctacatt
     5521 ggccgatcgt attttagaaa tgacgcacac tgtgacgtct cgcgaaatgc aagatcagct
     5581 gttagactcg atggatttgg aacgtgaacg tggaatcacg attaaattaa atgctgtaga
     5641 actcaattac acagcgaaaa atggagaaac atatatcttt cacttgattg acaccccagg
     5701 gcacgtcgac ttcacctatg aagtctctcg aagccttgct gcctgtgagg gtgccgtttt
     5761 agtggtcgat gcagcacaag gaatcgaagc acaaacattg gccaatgttt atttagcgtt
     5821 agacaatgat ttggagatcc tgccagtcat caataaaatc gacctacccg cagcagatcc
     5881 cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc gatgctagtg aagctgtatt
     5941 agccagtgcc aaatcaggga ttgggattga agatattctc gagcagatcg tggaatatgt
     6001 tcctgcacca agtggagact tagatgcacc gctaaaagct ttgatctttg actctattta
     6061 tgatagttac cgcggtgttg ttctaaatgt ccggatcacg gacggtatgg tcaaacccgg
     6121 agacaaaatc aaactgatga gtaatggcaa aacatttgat gttacagagg tcggggtctt
     6181 ctcgccaaaa gcagttgcta gagacttttt gatggtcggt gatgtcggtt atatcacagc
     6241 aagtatcaaa actgtccaag atacacgagt aggggataca gtaacgttag cagacaatcc
     6301 tgccgctgaa gctttaccag gttaccgaaa aatgaatccg atggtttatt gtggtcttta
     6361 tccaattgat acttctcgtt acaacgattt gcgggaagct ttagaaaaac tgcaattgaa
     6421 tgatgctgct ttacaattcg aaccagaaac ctctcaagca ttaggttttg ggttccgctg
     6481 tggattcctc ggacttcttc atatggatgt cgtccaagaa cgcttggaac gggaattcaa
     6541 tctagaattg atcacgactg caccatccgt tatttaccat gttaataaaa cggatggtac
     6601 gactgctaca gtagataacc cggcagattt tcctgaacct gtaacgattc aggatgtaga
     6661 agaaccattc gtcaaagcac agatcatggt cccaaatgat tttgtcggtg cagtaatgga
     6721 actttctcaa cgaaaacggg gagaattcat tacgatggat tatttagatg attaccgagt
     6781 aaatgtcgtc tataatattc cgctttctga aattgtcttt gactttttcg acaaattgaa
     6841 atcaagcacg aaaggctatg cctcattgga ttatgaaatg tctggttatc aaaaaagcaa
     6901 gttagtgaag atggacatct tgctgaatgg agaaaaagtc gatgcattaa gctttatcgt
     6961 tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt gaaaaactga aaaaactaat
     7021 cccacgccaa caatttgagg tgccaatcca agcagctatt ggacaaaaaa tcgttgcccg
     7081 ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa tgttatggtg gagatgtttc
     7141 tcgtaaacgt aaactcttag agaaacaaaa agaagggaag aagcggatga aacaaatcgg
     7201 ctccgtcgaa gttccgcaag aagccttcat ggccgttctg aaaatggacg aggacgaacc
     7261 gaagaaatag caagggtttg tttgaaaaca gtgtaaaaaa taaaccatgt ttacatggat
     7321 ttgccaccgg tttgccaccg gtggttt
//
LOCUS       DAXWLG010000124         7253 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXWLG010000124 DAXWLG010000000
VERSION     DAXWLG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.125"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            99..746
                     /locus_tag="ACGE4T_002094"
     CDS             99..746
                     /locus_tag="ACGE4T_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8387336.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACGE4T_002095"
     CDS             1139..3784
                     /locus_tag="ACGE4T_002095"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFF8387337.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACGE4T_002096"
     CDS             4094..5407
                     /locus_tag="ACGE4T_002096"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFF8387338.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACGE4T_002097"
     CDS             5397..6050
                     /locus_tag="ACGE4T_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8387339.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACGE4T_002098"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACGE4T_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFF8387340.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACGE4T_002099"
     CDS             6947..7147
                     /locus_tag="ACGE4T_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8387341.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2396 a         1229 c         1593 g         2035 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt att
//
LOCUS       DAXWLG010000125         7231 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXWLG010000125 DAXWLG010000000
VERSION     DAXWLG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.126"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(306..731)
                     /locus_tag="ACGE4T_002100"
     CDS             complement(306..731)
                     /locus_tag="ACGE4T_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387342.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1105..2076
                     /locus_tag="ACGE4T_002101"
     CDS             1105..2076
                     /locus_tag="ACGE4T_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8387343.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2108..3505
                     /locus_tag="ACGE4T_002102"
     CDS             2108..3505
                     /locus_tag="ACGE4T_002102"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8387344.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3664..4287
                     /locus_tag="ACGE4T_002103"
     CDS             3664..4287
                     /locus_tag="ACGE4T_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387345.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            5036..7129
                     /locus_tag="ACGE4T_002104"
     CDS             5036..7129
                     /locus_tag="ACGE4T_002104"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8387346.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         2456 a         1214 c         1468 g         2093 t
ORIGIN      
        1 ttgaagttca taaaaatcag ccatagtgag ttttgcttgg atttgtttat cttcttctac
       61 tttttacata attttgaaca ggataagatt caaatgtttt aaatgaatct ttgactagtt
      121 tgtgacagtt gtcgcatatg ctcaagcttc cttggtcatt aaattctgta tggatatttt
      181 tgttgttatt acatgtacaa taatcaggac tatcgatcat ccatttcgtc tcctttacaa
      241 tcatcttgct tctttactat agcagaaaag aaggggagaa acaaaagaag atccaacaga
      301 cggcactata gactgtagat gttgccatcc tcatccttca aacggtaatt accgattttt
      361 gtaaatatga ctgttgtttc ttttttattt tcttttcctg aaataatata ttcgtcatct
      421 ttggtagtga tcttgatatc tgtcagcact gttttttctt cagatgattc agaagaatca
      481 gttccttgtg tctgatacgt gcgtgtccat tccatttttt tatctgtaaa tgtcaactgt
      541 cccgcggata aatccatatc tttacgtact tttagcgtat atacactgtt ggtggtatct
      601 gctttttccg cttttaagac agaagaatcg gttgtttcgt ccgaggattg gtcagagccg
      661 ccaaacaaat cacagccagt caatgtcaaa agtcccgcag acaaaataaa tagtacgatt
      721 aatttcttca aaatacttcc cccatttgat aggataaaga aattatacca ttggaatgta
      781 aaacttcaaa cggatggaag agaaaaacgc acaaaacacc cacaaataga gtaaatttta
      841 gaataaaatt ttaaaaaggc aaaaaatccg ccactttcag aaagcgtttt aaaaaagtgt
      901 tgcaatcttt taaaaaaatg ctatattaaa aatgaaccgg ttcaaatact tgtttaagta
      961 taattaatag tgagaggaag ttgctctact gaagacttta gttatatgga tattgatgac
     1021 aagcaacact atgcaaagaa tgcggtcatc ataatcgaac aacaagaaag agaaatcaaa
     1081 tgtaactggc aggagtgaga atgtatggca tcgatccgtg atgtggctaa gatggctggt
     1141 gtttcagtgg gaacagtttc ccgttacttg aatggtcagc aactgaagga aaagaacatg
     1201 aaaaaaatag cagaagcgat ttcagcgttg gactataaag aaaacatcat tgctaaaggg
     1261 ttgaagaata atcgaagctt ctctattgga cttttgataa acgggatttc cagccgtttt
     1321 gggtcggaag ttgtctcagg gatcgaaaga gtagctgaag aaaatgggta tagcttatta
     1381 ttaagcgggt ttgccgatca gccagaaaaa attgaacaaa aaattgaata cctgatgaag
     1441 catgtgatcg atgggctgat tgtcttctta tccgaagaag agtggtctgg atttgaaatg
     1501 ctcacaaaaa tgagtattcc agttatcgca ttgaattgtc cagaaggacc agcaggtatt
     1561 gatacaatat tagtgaatga tcgagaaagt gtagcaaaag tgatcgctca tcatggtgaa
     1621 caaggacata aaaaaatcgg atttattgct gccactcaaa cagactacgt agcaagagaa
     1681 cgtttagcag gagccaaaga agcggtccaa aatgatccat cgatccaatt agaggtcttt
     1741 acaggagact attctagaaa aagcggctat tacggggcaa aagcattatt agaaaaaggg
     1801 gcaactgcta tttttgtcag caattacaat atgtcgattg gtgccatcga atgtttcaat
     1861 caagaaggtg tcagaatcgg gaaggatatt gcgttcagtc actatgatta tttagataaa
     1921 aacgatctga gtatccttcc gaaaatcacg atcacgccac caacagataa aatcggggaa
     1981 cgagcagcag aacagctgtt aaaacgaatc aaaaaagaag aggaagaaaa tgaaaaagtg
     2041 attgtgatgg aaaatatcat acatggaatc aactaaaacg aactaaaaaa gaaaaagagg
     2101 tgctttgatg aaaaaatatc aatttccagc aaatttttgg tggggttctg cagcaagtgg
     2161 cccacaaaca gaaggaagga tttcaggcga tggaaaagga gagaacattt ttgattattg
     2221 gtacaaaaaa gaaccgcaaa agttctttga tcaagtagga cctgaaaaaa cctcgcaagt
     2281 ttatacgaag tatcaagagg atgtacagtt gatgaaacag actgggcata attcatttcg
     2341 aacatctatc caatggagcc gtttgatacc tgatgaatct ggaacaatca acgaagaagc
     2401 cgttcgtttc tatcatcgtt attttgatga gttgattgaa aacggtatcg aaccctttgt
     2461 gaatctgtat catttcgata tgccgatgta tctgcaagaa aaaggcggat ggttgaaccg
     2521 agaaacggtt gaagcctatg aaaaatatgc caaaagttgt tttgaattgt ttggttcgaa
     2581 agtaaaaaaa tggtttactc ataatgaacc aatcgttcca gtcgaagcag gttatttata
     2641 tcgatggcat tatccagaag agtcagatat gaaaaaagcg atccaagttg gctatcatga
     2701 agcgttggct tcagcgttag ccatcaaatc ttaccatgaa atggagcaag gtggtgttat
     2761 tggtatcatc ttgaatctga caccaagcta tccaagagac gaatcaaacc ctgaagatgt
     2821 taaagcagcg caaatagcag atgcgttctt caatcgttca ttccttgatc cagctgtaaa
     2881 aggcgagttt ccaagtgaat tgattgagat agtaaaagaa cttgatctca tgcctgtgat
     2941 ccatgcagag gatttgacgg ttatcaaaga aaatacagta gatcttttag gtatcaatta
     3001 ttaccagcca cgccgggtaa aagcaaaaga aacaccaatc gatactaaaa acggtccaat
     3061 gcctgaggat tatttcgaca attatgaaat gcctggtcgt aaaatgaatc cttatcgcgg
     3121 atgggagatc tatgaacaag gaatctatga tattttgatc aatgtacgag atcattatgg
     3181 aaatatcgac tgctttattt ctgaaaacgg tatgggtgta gaaaacgaag cacgatttat
     3241 caaagaagat ggcatgatcc acgatgatta tcggatcgaa tttgtccaat ctcatctgaa
     3301 atatgtccat caagccattc aagaaggtgc caattgttta ggctatcata tgtggacatg
     3361 tatggataac tggtcgtgga ccaatgcata taagaatcga tatggcttta tttcagtaga
     3421 cttagcacaa gatggaaaaa gaacaatcaa aaaatcgggt cattggttca aagacgtatc
     3481 agataaaaac ggatttgagg cttaagaaag aatagttgag agcaaatgcg tgaaattctc
     3541 cattcttacc attttgtgaa aaagaagttc aattatttac gatctttttc aacttttttg
     3601 tctttctatt tcattttttg tatataatag aatagtcaaa attaaatagg agtggaggaa
     3661 agcatgagga aaaaaagacc gtttcttctc ttttggttat tcggtttttt cttactgctt
     3721 attttatccg cagcattttc aggaaaaatg acagcagatc ttgctcagaa agaaacagaa
     3781 aaaatcgtag caagcaaaga atcagtagat aatttcaaaa aagaattctc aacttattgg
     3841 tgggcggagt ataaaataac tgccaattac gaaaaaggcg aattggacat ttatttgcca
     3901 aaaggaaaag aagaaaatca atttattaat atcgatgacg tacttagtgc gatttctctc
     3961 agacaatatc aatttaaaga caagcatttg accgttttgc ttttgactca tgatggcgac
     4021 aaagtagccc gtttgaaaaa tggaaaagtg atcaccgctt attctgaaaa agtagacaaa
     4081 aaagtggtcg ccgatcgttt agcggaatgg aaaaagacaa atgaacaagt caagaaaaca
     4141 actgtctctt ctcaaaaaaa cgagtcgaca ggaacaagtc cttcagattc tggcagtgga
     4201 caaacgataa cgggaagtga agaaaatcaa gcctcaatta ttacgaatga gtcatcacaa
     4261 ggacaatatc aatcctatgg acaatgaaaa acagaggttg tgaaatcagc gttttgacct
     4321 gagtattaga gaaacaaacg gcaaaatagt tcctcatatt ttgccgtttt ttgagctaat
     4381 accgaaggtc agctgattga acaccgtgta tcaagaggtc ttgagcctgc cgtttaactc
     4441 tgagccacaa ggagcaattt caaaaaatag ctccttatat ttttcgtttg gttgggctta
     4501 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttgtaaaat tagcgtcctg
     4561 acataatcta ataccgaggg tcagctgatt gaacaccgtg tatcaagagg tcttgagcct
     4621 gccgtttaac tctgagccac aaggaacaat ttcaaaaaac agcttctcat gttttataaa
     4681 tattgtgact tgtggccgag gagttggcag gagaacaccg tttaaggaag aagttgtgaa
     4741 aaagctgtcc tgcatcaaga aataaggagc tgcgacaaga attttgtcac agcctctttt
     4801 tttgtacgaa taccgagaaa ctggcttttg aataccgtgg agtgaagcgg tcaccacaag
     4861 acttttatct cttcattagt ttaatcgctt acatcgcagc ggttttatgc tgtgaaaagt
     4921 ggacggaata atctttagaa agaataaaaa aatcttaaga taacaacttt tttaggaaat
     4981 tttcattttt caggagcaaa ataagaaaaa atgtgaatag agagaagtga tatacgtgac
     5041 acaacttaaa aaattgtgcc aaaatcgttt agcaatcgtt ttgacagctg ttttcttttt
     5101 ctggctgaag acgatcattg cctactatgc agatttttca ttaggtgtgg aaggtacgat
     5161 ccagtacttt atcttgtgga tcaacccaat tgcgactacc ttattgtttt ttggcctttc
     5221 tttgtatatt aaaaagccaa agccagcgtt agctattctt ttgatcattg atattttaaa
     5281 tacgcttttg ttgtacttga atattatttt ttatcgggaa ttcacagatt tcattacagt
     5341 caaatctgta ttaggctttt cgaaagtgtc gcaaggattg tcaggaagct ccttttcttt
     5401 gatgaagccc catgatgtca tctactggct ggatatcgct gtgttcattg ggctactggt
     5461 ttggttgaag gtcaagaaaa tacctatcaa aagcaaccct gtcggcaaac cgatggctat
     5521 cgctgttacc tgtctttcag ggcttatttt ttcaggaaat ctagcgctga gcgaagccaa
     5581 ccgtccacaa ttgctgcagc gaacatttga ccgttcatat attgtcaaat atctaggaat
     5641 cgatgcgtat actatttatg acggaatcaa aacaggaatg actagcagtg ttcgcgcgca
     5701 tgcaagcagt aatgggattg atgaagtttt agattataca aagaaacatt atgcagagcc
     5761 aaatcccgaa acatttggga tcgctaaagg gaaaaatgtg atcgttctcc atttagaaag
     5821 tttccagcag tttttgatta atatgaaggt agacggacaa gaagtcacac cgtttttaaa
     5881 tagtattttc cagaatcaag caacgatcag ctttgacaat ttctttcatg aagtaggaca
     5941 gggaaagaca agtgatgctg aaaatatgtt ggaaactggc acatttggtt tacctcaagg
     6001 ctcattattc acagaacttg ggtcagacaa tgtctttcaa gccgcaccag cgatccttgg
     6061 acaaaagcaa ggctatacaa gcgctgtttt ccatgggaat gtagctagtt tttggaatag
     6121 agatcatgta tataaaaact taggttatga caatttcttt gatcgatctt attttgatga
     6181 atccgacgaa acgttaggtt atgggatatt agataaagat ttattcagag aatctgccca
     6241 atatcttgag catctacaac agccgtttta cacgaaattt ctttccgtaa ccaaccacac
     6301 gccttattat acggatgaca agaatttcga ctttccatct ttgaatactg gcaatagcac
     6361 agtagataat tatgttcgaa cggcacatta tctggatcaa tcattggaac aattttttac
     6421 gtatttgaaa aaatctggta tttaccaaaa ttcaatcttt gtgatttacg gggatcattt
     6481 tgggatctcg aatacagata ataaagattt agcaagcgct cttggtaaag atccagatac
     6541 atgggatgaa tttgacaacg cacagatgca acgagtgcca ttgatgatcc atatgcctgg
     6601 ttatactaaa gggacagtca atcatgaata cggtggagaa atcgatgttt tgccgacatt
     6661 actacatcta ttgggaatcg atgataaaga ttatcttcat tttggtacag acttattatc
     6721 ttctcagcat gatcaagtag ttgcttttcg aaatgggaac tttgtgacac ccaaatacac
     6781 cgtcctagga ggaaaagctt acaataatca gacaggagaa atcatcgata cgaaagcagc
     6841 aggaatcgat gaggaaatct ccaaagatca ggaaaaagtc aagactgcgt taagtctttc
     6901 agataagctg aatcaggaaa atctcttgcg gttttatgtg ccaaacggat ttgagacgat
     6961 tgatccgaaa aaatatgact ataaaaatga agcggaacgt aatgaagcaa tcgaaaagca
     7021 aaaaggcgaa cattcgacaa gtgtcttttc aaaaaatggt aataaaacaa caaccaactt
     7081 atatccagta acaagaacag acgttgaaca gcaaaaaaat aatcaataaa caatcaaacc
     7141 aaccttcagt agttagacaa cattctaatg cttgaaggtt ggtatttttt atgaaaaaaa
     7201 cattttttta tttttttcgt gtatttttcc t
//
LOCUS       DAXWLG010000126         7226 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXWLG010000126 DAXWLG010000000
VERSION     DAXWLG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.127"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            70..153
                     /locus_tag="ACGE4T_002105"
     CDS             70..153
                     /locus_tag="ACGE4T_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFF8387347.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..1015
                     /gene="erm(B)"
                     /locus_tag="ACGE4T_002106"
     CDS             278..1015
                     /gene="erm(B)"
                     /locus_tag="ACGE4T_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8387348.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1185..1445
                     /locus_tag="ACGE4T_002107"
                     /pseudo
     CDS             1185..1445
                     /locus_tag="ACGE4T_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1608..2273
                     /locus_tag="ACGE4T_002108"
                     /pseudo
     CDS             1608..2273
                     /locus_tag="ACGE4T_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2309..2554
                     /locus_tag="ACGE4T_002109"
     CDS             2309..2554
                     /locus_tag="ACGE4T_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8387349.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2657..3526
                     /locus_tag="ACGE4T_002110"
     CDS             2657..3526
                     /locus_tag="ACGE4T_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8387350.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3507..4241
                     /locus_tag="ACGE4T_002111"
     CDS             3507..4241
                     /locus_tag="ACGE4T_002111"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8387351.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4274..5182
                     /locus_tag="ACGE4T_002112"
     CDS             4274..5182
                     /locus_tag="ACGE4T_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8387352.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5179..5721
                     /gene="sat4"
                     /locus_tag="ACGE4T_002113"
     CDS             5179..5721
                     /gene="sat4"
                     /locus_tag="ACGE4T_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8387353.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5814..6608
                     /locus_tag="ACGE4T_002114"
                     /note="aph(3')-IIIa"
     CDS             5814..6608
                     /locus_tag="ACGE4T_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8387354.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6884..>7226
                     /locus_tag="ACGE4T_002115"
     CDS             6884..>7226
                     /locus_tag="ACGE4T_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8387355.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEK"
BASE COUNT         2526 a         1087 c         1514 g         2099 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      481 agaaaaatta aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca
      541 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      601 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      661 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      721 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      781 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      841 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      901 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      961 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     1021 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
     1081 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
     1141 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
     1201 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1261 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1321 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1381 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
     1441 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
     1501 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
     1561 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
     1621 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
     1681 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
     1741 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1801 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1861 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1921 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1981 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     2041 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     2101 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     2161 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     2221 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     2281 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     2341 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     2401 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     2461 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     2521 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     2581 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     2641 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     2701 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     2761 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2821 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2881 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2941 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     3001 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     3061 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     3121 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     3181 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     3241 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     3301 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     3361 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cactttttga
     3421 atgctacgac atgaccgaga agctttataa tgaagtgaat gaaattgtat cggagataaa
     3481 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
     3541 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     3601 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     3661 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     3721 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     3781 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     3841 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     3901 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     3961 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     4021 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
     4081 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
     4141 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
     4201 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
     4261 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
     4321 acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc
     4381 taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat
     4441 atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga
     4501 tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta
     4561 caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga
     4621 taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac
     4681 tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga
     4741 attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc
     4801 tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt
     4861 cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta
     4921 tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa
     4981 catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc
     5041 ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga
     5101 catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat
     5161 agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat
     5221 aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat
     5281 tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag
     5341 gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga taagataata
     5401 tatctttact accaagacga taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac
     5461 cggtacgctt atatagaaga tatcgccgta tgtaaggatt tcagggggca aggcataggc
     5521 agcgcgctta tcaatatatc tatagaatgg gcaaagcata aaaacttgca tggactaatg
     5581 cttgaaaccc aggacaataa ccttatagct tgtaaattct atcataattg tggtttcaaa
     5641 atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt
     5701 ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa
     5761 ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta
     5821 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     5881 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     5941 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
     6001 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     6061 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     6121 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     6181 actccatcga catatcggat tgtccctata cgaatagctt agacagccga ttagccgaat
     6241 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca
     6301 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     6361 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     6421 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     6481 tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     6541 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     6601 tgttttagta cctagattta gatgtctaaa aagctttaac tacaagcttt ttagacatct
     6661 aatcttttct gaagtacatc cgcaactgtc catactctga tgttttatat cttttctaaa
     6721 agttcgctag atagagttct atattagaga tgtaaagagt tttggtgaag aggtttgtga
     6781 gtatggcttt tataaccagg gtgcaatgag aagcactaac agcagctagc ttaagaacgc
     6841 ataggatata ctttttatgg aagtcacaga ggaggaataa aatttgaaca ggattaacag
     6901 agtaacctct attcttattc agctgcaatc aaaaaaaata attcctgcca aagaaattgc
     6961 acagcgattt aatataagct taaggacagt ttacagagat atccggacac ttgaagaagc
     7021 cggaatacca attggatctg aggccggaaa aggatatttt cttgtagagg gcttcctact
     7081 tccgccggta atgtttacag cggcggaagt gggtgcattg attacagcag ggaaattttt
     7141 aaattgccat ggagatgaat catttataaa ggattttgat tcagctatgt ataaaatcaa
     7201 atctattttg aagcatggtg aaaaaa
//
LOCUS       DAXWLG010000127         7202 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXWLG010000127 DAXWLG010000000
VERSION     DAXWLG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.128"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            33..1043
                     /locus_tag="ACGE4T_002116"
     CDS             33..1043
                     /locus_tag="ACGE4T_002116"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8387356.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1112..2056)
                     /locus_tag="ACGE4T_002117"
     CDS             complement(1112..2056)
                     /locus_tag="ACGE4T_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8387357.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2040..4760)
                     /locus_tag="ACGE4T_002118"
     CDS             complement(2040..4760)
                     /locus_tag="ACGE4T_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8387358.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4757..5977)
                     /locus_tag="ACGE4T_002119"
     CDS             complement(4757..5977)
                     /locus_tag="ACGE4T_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8387359.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6258..7103)
                     /locus_tag="ACGE4T_002120"
     CDS             complement(6258..7103)
                     /locus_tag="ACGE4T_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8387360.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         2130 a         1372 c         1146 g         2554 t
ORIGIN      
        1 aataataact aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag
       61 ctactagtgc gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata
      121 tcgcaacgat gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg
      181 aatcatcgaa ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat
      241 atggcgataa agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat
      301 taggtgatac ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag
      361 aatatattcg taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag
      421 atgacgattt gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca
      481 aattatcaag tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt
      541 tctctaaatt agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag
      601 ttaaattcga ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat
      661 tgaaagacgg tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag
      721 aatactacgt tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca
      781 aagatcaact aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta
      841 aagtaatcgg tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa
      901 atgtgttgag tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag
      961 cttcaacaaa gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag
     1021 aaacaactga ttcatcaaaa taaaagaaaa caaaaaaagc gaagacttat gtctcgcttt
     1081 ttttgttttg aatgaaaaaa ataaattttt gctataaatc ttttggtaca tagaaacttc
     1141 ggttgtccat accgaaaatc cgatcggtgt attttcctgg ttctgtttga cgaatcgaag
     1201 tcaacatcat ctgcatcgaa gcatcgatat tgtcgatttg atgtaatatt tctgcttcca
     1261 ttattcttgg acgtacaggt gagccatatt ctaataagcc atggtgtgct aaaaccatat
     1321 gacgtaatac gagaacatct tcgtcagctt cgctgatttt caacgcaaga caggctttag
     1381 ttatttcttc gtcaatcaat accagatgac cgatcaaatt tcctgcaagc gtatattctg
     1441 tcgacatagg accggaaagt tcaatcacct ttcctaaatc atgcaaaatg ataccagcat
     1501 acaacaatgc tcgattcagc tcaggatatt catcggcgat tgatttccct aagtgaagca
     1561 ttgtcaaagt atgataagct aatccactgg caaatgcatg atggtttctt tttgctgccg
     1621 gaaactcaaa aaattgtttt tgatatttat taagtagata gcgaacaata cgattccaat
     1681 gcgcattggt gatctcaaac aacaattgat tgatttcttc ttccatttct tctttcttca
     1741 aaggggctct ttccatgtat aaactaggct ctcctggctc atctaccctt gttaagcgca
     1801 tatgaagaat ctttacttga gggtttcctt gataaacctc tcttttaccg tttagaaaaa
     1861 cgactgtgcc ggctgtaaat ttattgattt cctcctccga agcatcccag tatttaccat
     1921 cgatagtacc tgaagtatct tggaaagtaa aagcaataaa tttttttccg tttttagcga
     1981 ttcttacatc tgcacttttg atcaggacaa attgttcaaa ctgctcatcg attactaaat
     2041 cacgaatttt tttcattatt tgctccttct aattgtttta cgatttggtt catctcatgg
     2101 taatagctta ccatttcttt atcagaagaa aaacaaatta tttgatactt ttctccaaat
     2161 ttagctaaaa gttcagctaa ttgatacttt cgctgatgat cataatgaag ccagccatca
     2221 tcaatgatga tcgggcatat cgaacggtta gcttctaatg agaggtaagc aaatctcatc
     2281 gccataatca gctgatcttt cgtacctgta gataactcat agatgggaaa ttctgtatat
     2341 ccgtctgtta aagtcagtgt atcatttttc agtaatatct tctgataatt tccttttgtt
     2401 aaaataccta aataatcacc tgctgctctt aacaactggg gtaattgtct ttcactcatc
     2461 tctgttgaaa gatcacctaa gaatgtcccg gcgatttcaa gtgctcccca ttcctccagt
     2521 aattcctgta attttgcttt ttgccggcta gcttgttgat aaagttcgtc caatgtgccg
     2581 tctctttgca actcttctac ttgaagttgg agtcgttgcc gattttcact tgcttctttt
     2641 tcttcttgct ggagttggga ttgcttttct ttcagttggc gcagtcttac agataatttc
     2701 tcttttgtca gctcttcagg aaatagtgga gtaagcattt ccgataattc attgtatctt
     2761 ttcatctgct gttcggcttg tttggctttt tgtaaaaaga caggtacttc cgaaggataa
     2821 gataagcctg cttgagtcaa ttgttcgctg tattcttgta tccatacttt ttgtttcttt
     2881 ttcaattgat tgatttcttg cttcaataaa gtattttgct gatagcttct ggcaaatttc
     2941 atctgttcca tctcttgtat aaaacgggaa agcaatacca ttttttctga aattgtctta
     3001 tcttgtatcg gtaaccattc tttaacaaac tcaaattttt ggtcgatttg tcgtagtttt
     3061 tcttgtattt cctgttctct tttatgtttc cgctcaaata cgccatataa tttctgataa
     3121 tctatgactt cctgtccata ttctttcatg agtttgagcg tatccatctt tcctaagtgc
     3181 cgatgatccg caaaacgata tacttcttgc tgaagaatct gtttttcttc tccagttctc
     3241 ttttgttttt cttctgcttc agctaattga gctaaggcta gatcaagttg tcctagtttt
     3301 tcttgccaca ttccttttat ctcagagaaa tcatttttag ttttaatttt tggttgcctt
     3361 ttgtagaaga aatagccaat catgcctgca ccaattagta aaaggatcac tttcaagggc
     3421 attggtacaa atatacctgc aatgattatt gctccgccag ccatcagcat atacggttta
     3481 gatgaagaga ctggtgctat ctctgactga ttttgtaata atgctggata ggtctcttcc
     3541 aagtcattca cttctgcttc aatcatctct tgctgttctt tagctatttg gagacgcaca
     3601 gaaatctggc tttctagatc agcaatcctt ctttcttcag ccagaagatg cttccattct
     3661 tcttcatata aaaattcttc cggtaacgga tttttctgcc aatgccacct ttgttccaaa
     3721 atcttcaatt ctgaatttgt ttctctagtc tcaatttgta atctttgcca ttcttcgttt
     3781 aattgttccg ctgtaaattg aagttgctgg agatctttta gttcctgctc ctgctctaaa
     3841 taaaaataat atctggctga ctgctgatcc atcccgctat gcttttccag tgttgcttct
     3901 aattcttcca aacgctcatt tgtttgctga taccgtttgg agaaagactc cagatctaaa
     3961 agaagttgct catcaggaag acctgtagtg ctgttttttt tgagagattg ccattcttca
     4021 tataaagaaa aattaagttc ttgctgtctg gctaatgaaa attgtttgtt cgcctctttt
     4081 aattgtgcca ataactgctg ctgttgcgac tcgtattcct gttcttgttg gatcaacttg
     4141 ccaaaataag cttcctgttc ttgtttttga tgaatttttt cttgcagctc tttccattca
     4201 tttaattttt gattgattag taatttttgg ccttttttct tgaataattg ttgtgctttc
     4261 tgataatatt cttgccgctt ttggaatact tgagaactgc ccgtgatgcc taaagaaatc
     4321 agcgcatcat gtagctcttt ttctctcaaa tgatctagtt ccatcaactg ctcttgctgg
     4381 aaagtaaata cctgctggaa taactctttc gtcaaaggtg cgatcagttt ttctagtaac
     4441 tcatcacttc ccacttgatc ccccagccac accttcgatt ttccgcgatt ttgctgcttg
     4501 tagcgctcga cagcaaattc accatataca ggatgtttaa gccatatttt gccgccatag
     4561 gctgagccgt cttttggtgt ataatccttt tttttacggc cttttgaagg gaaaccaaat
     4621 aatattgctt gaataaattg ataaatcgtt gattttcctg cttcgtttcc cccatagatc
     4681 agctgattgc ctgaataaaa gtcaaaactt ttttgacgcc attttccaaa agcagcaatc
     4741 tcgatcctta aaatcttcat tcctgatcct cctcaaataa aaaatcttga ttaagcagct
     4801 gcttggcttt ttcaagtgtt tcttcttgat attccggcag ttcgctcaat attctagccg
     4861 cttcttgatg actatatact tcatttaaaa tttgttggaa atcttgaggt gtctgatagt
     4921 attgaaataa ctgttccatc aatttagcag aagcagcaag tggggtcttc gtataaaaag
     4981 tattctctat gagagaaata cgccaaataa agaaatcatt tatcctatct gagaattcct
     5041 ctaacaaata gtctaatagc tctcctgatt gtatccgttc caaaacatct gtattgagat
     5101 ggtcataatc ttttaatttt atctcaatta aactaaatcc atcctctact tttgaaagct
     5161 gctgacgaat gtgaacaatg acctcttttg tcgttcttgc tttcctcaat gatatttcct
     5221 ttgtcttcca atagacctct gctactttaa tcggatgtat ttggcaactt cctttactta
     5281 attcaactag tagaatagat gtagaagctt gttccttttt agtatgacct tgaggcgcac
     5341 ctggataaac aattgtttgc ttttcattta agaccgttgg cacatggata tgccctaaag
     5401 cccaataatc gtatcccttt tcttgcatct ttgagggttg gaaaggagca tagtttcctt
     5461 tttgagagac gccaggttcc ccgtggtaca acccaatatg gtaatctgtg agttctctag
     5521 aaggaaattc catcactttg tctttttgaa tccacggttg acgataacta aatccactga
     5581 gagagacagt ttctccactt tttatcgtta ttttttttgt ctctaccgtt tcggatgtaa
     5641 ataaatggac atttttagga aaggagaacc aataccgctc tttttgatag aaatcatgat
     5701 ttccgaatat tataaataca ggtatatggt tcttttctaa ccgtttcatc tgttctgaaa
     5761 aatgtttctg tatttttaat gaaggacgat tttgatgaaa attatctcct gcaagtaaaa
     5821 caaaatcaac gttatttatt attgcctgtt caatgatatt tgataaaacg gtcagattcg
     5881 tcttcaatag tttttcctgt acagttttat caagagttgt cagtccttca aatgaccgat
     5941 ccatatgtaa atctgccgca tggataaatt tcaccattaa aaatttcctc ctttttttct
     6001 tttatcatac cattttcaat gttttttttc atcaataacc gaaaatttgt tcgtattatg
     6061 caaagaaatt acgataaatg gccgacttca aaaaataagt ttattcgaaa aaaagattgt
     6121 gacatgattt ttgtcacaat cctaaatatc tatgttatat tcgcttcgtt tgacactgaa
     6181 gaagtactat ttgtactgga atttatgctc catttttccg tatttagaat tctatgttct
     6241 atactacctt aattagttta tatggtaaga attctttcaa atgcttgaga tactccgtct
     6301 ttatcatttg aaacagtgac ataatcagct tttttcatga tttcttgatg actatttccc
     6361 attgcaaaac taatatctgc actttcaaac atagataagt catttaaact atctccgatc
     6421 gcgattctca aaggtttctt taaattgtag attgattcca aacgatcaaa caccacacct
     6481 ttagatacat tttgaggtac gatttcaata tttacaggtg ccgattcagt aacagacaca
     6541 tttagcatct ttttcaaatg gttaaatata ttagctttca attgacgatt aggaataaaa
     6601 ataaaaaatt tgtaaatgct gatagcatcc ttttctataa tttcctcaat attattataa
     6661 gggattgaac aaatagattt ttcatactca agctcatagt ttagctcttt gatagaaaat
     6721 tcactcccta cctcgtgaaa aatttttttc attttatttt tataattctg agattcataa
     6781 cttccgagat ttgtatatat tttggttgga aatttcgata ggacagaatc aatttttttt
     6841 acatcatttt tatcgaagga agactcaaat atcttttttt ctccatcaaa tccaacagaa
     6901 ccatttaacc ctactgctgg tatccttaaa ccagattttt tgagaatatt attaatgtct
     6961 tctacttctc ttccagaaca aatgaatgga aaacagtctt ccggtaaatg tttcattgtt
     7021 cttgcatttt tttgactaat gctctggtca gaattcaaaa gagtaccgtc taaatctata
     7081 ccaattagtt tcatttcatc catgtctcct atacgttgat tataaattca ctgcttattg
     7141 taagtctaaa tttctcacaa aacatatact aattatacct aatttaagta agtacaaaca
     7201 tc
//
LOCUS       DAXWLG010000128         7023 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXWLG010000128 DAXWLG010000000
VERSION     DAXWLG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.129"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            280..1137
                     /locus_tag="ACGE4T_002121"
     CDS             280..1137
                     /locus_tag="ACGE4T_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8387361.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1130..2032
                     /locus_tag="ACGE4T_002122"
     CDS             1130..2032
                     /locus_tag="ACGE4T_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8387362.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            2065..2415
                     /locus_tag="ACGE4T_002123"
     CDS             2065..2415
                     /locus_tag="ACGE4T_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8387363.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2464..3387
                     /locus_tag="ACGE4T_002124"
     CDS             2464..3387
                     /locus_tag="ACGE4T_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8387364.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3465..4358
                     /locus_tag="ACGE4T_002125"
     CDS             3465..4358
                     /locus_tag="ACGE4T_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8387365.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4396..4959
                     /gene="amaP"
                     /locus_tag="ACGE4T_002126"
     CDS             4396..4959
                     /gene="amaP"
                     /locus_tag="ACGE4T_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8387366.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4973..5161
                     /locus_tag="ACGE4T_002127"
     CDS             4973..5161
                     /locus_tag="ACGE4T_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8387367.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5174..6076
                     /locus_tag="ACGE4T_002128"
     CDS             5174..6076
                     /locus_tag="ACGE4T_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8387368.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6108..6350
                     /locus_tag="ACGE4T_002129"
     CDS             6108..6350
                     /locus_tag="ACGE4T_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8387369.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6606..6977)
                     /locus_tag="ACGE4T_002130"
     CDS             complement(6606..6977)
                     /locus_tag="ACGE4T_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387370.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2565 a         1192 c         1397 g         1869 t
ORIGIN      
        1 atgagaattt aaaaaaatga aactaacttg ctttgtcatt atgaattttt tcaaaaaaca
       61 ttcttaagaa aaaggtggct atatatagta gtttttggtc aatacctttg aataaaaagc
      121 catgaaaaac gaatagttag cgcttaccga tgcgttttgt gataaaaaaa ttttaaaata
      181 ttcaatgaaa acaaagcata tattttgctt taaaaccaaa tgaggaatat tctagatgta
      241 gaacaggtta gaaaaagatt agaaaaagga gtgattttga tggagaagag atacggcgta
      301 gttcctgtgg taactgcttc agacgaaaat tatgctcctt acttgagcgt gatgatcgca
      361 actgcattag aaaattgcaa taaaacaaga cgcataaaat tttatgtgat tgacgatggt
      421 ctatcagaat atagtaaaga aggattggaa gaaacggtaa ataaatattc aagtaatgca
      481 agtatccaat tcctgacagt ggaaaaagat atctacgaag acttcctagt cagtgaccat
      541 atcacaacta ccgcttactt aagaatttct ttaccaaact tattagctaa agaggattat
      601 aagaaagtcc tttacttaga ttcagatgtg ttagtactag acgatatcgt aaaactatat
      661 gatgagccgt taaatggaaa aacaatcggc gctattatcg atccaggcca agtgaaagct
      721 ttggaaagat taggaatcga ttccgatgac ttgtacttca attcaggagt gatggtgatt
      781 gacattgatc aatggaataa aaaagaaatt acagaaaaaa ctatccatta cctcagtgaa
      841 aatggcgatc gaatcatcta tcacgaccaa gatgctttga atgctgtact ttatgaagat
      901 tgggaacaac ttcatccaaa atggaacatg cagacttcgc tgatttttga acgtcatcca
      961 gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa atgaaaaacc ttcgatcgtt
     1021 cactttacag gacatgataa accttggaat acgttgaaag atcatccgta taccaattta
     1081 tacttaaaaa aattagcaca cagtactcta acgaaagtag gcgagatcaa tgaataaaaa
     1141 agaaatagca gtagtcgcaa gttgtaacac aaaattcgta ccacatttag ctgcattatt
     1201 tgtctcagta ttagataatt gcaacccgtc taaatttgtt cggttctatg tgattgacga
     1261 tgatatcgat tttgaaagta aacagctttt acgcttctca gtgaaaaacg cacgaatgaa
     1321 ctcagatgtg gaattcttga aaatcaataa agaattcttt acaaatgtgg taatcagcga
     1381 tcgtattcca gaaacagctt attatcgtat tgcgattccg gaattgttcc gcggcacaga
     1441 agttgaacgt atcttatata tggattgcga catgatcgct ttacaagata tctctaaatt
     1501 atggcgtctt gacttcggtg attcaatcgt agcagctgtt gaagacgctg ggttccatca
     1561 acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt tatttcaact caggtttgat
     1621 gctgatcaat gtgaaaaaat ggttagatga aaatattacg caaaaagtat tagatttcat
     1681 cgaacataat ccagaaaaat tacgattcca tgaccaagat gcattaaatg ctatcttaca
     1741 tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa ggctatatca tggcaaaagc
     1801 aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa gaaactcgca acaatcctta
     1861 cattatccat ttttcaggac atgtgaaacc gtggagcaag gattttgaag ggccaacgaa
     1921 gaaatattac gaaaaatatg cagggatgac ggctttccgt tgtgtagcta aattccctaa
     1981 atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta gttgttaaat aatagaagac
     2041 cacaactgaa gagggggagt aggaatggaa attacaatgt ctaaaaatgc agtagagacg
     2101 ttaattgaaa aggttgggga aaatacaaaa atcgcactcg cgcttatcaa tgattccgat
     2161 ccatttctaa gggacaaagg tgcttttgca aaaggcagct tttttcaaat cattcctttt
     2221 gtttcggaat ttggagaata tgcgactaaa atcgagcatc cattattgga catctataca
     2281 tctaaattag agcaaaatta tttcggtaaa cgattgaaca tggacttcaa caaacagctt
     2341 gacagctttt cattagaaaa tgaaatcgca gtactcgatt ataatatcaa attaaagaac
     2401 tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga ggtatacatt gagatactac
     2461 tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa aaaaatataa ttggctcgta
     2521 cttgatagtg caaagcccga agagattgag caagcaatcg aagaattcga gcttcctgat
     2581 gatattttcg taggaaccga atatccggaa gaagtttctc ggatggagcg cctttatgag
     2641 acgaaactag agcatccatt ttctttggta gtgattaatc tagatcagtc agataatcgt
     2701 atcgaaaaaa aattaacgcc aatatctttt gtactttccg ataatctctt gattacttgt
     2761 atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa agcatggaaa tcatttaaac
     2821 tcgtttgaaa agatcatcac gtatacatta ttagatattt acacccatta cgtgaaagaa
     2881 ctaagagaga tgaaaaagcg aattgacgct ttggataaag cagctcgtaa aacaacggaa
     2941 aatgaagaac tgtataaaca ggcagatctt gaaagagaca tcgtatatat cgatcatacc
     3001 ttaagagatc aacgggaaac aatggatcaa ttatggaatt ctgaaacttt catcaaacgt
     3061 ttagacgatg aacagctact ctatgatgtc catttgagac agcgacaaac agaaaaaatg
     3121 atcgagatct atcgagattt attagagagt attggtgatc tattcaacgg gatgatggac
     3181 aataatctaa atcatatcat gaaatatcta gattcagctg cattaatcat ttccgttccg
     3241 gcgttgttcg cagggatttg gggaatgaat acaggaggat taccgggtaa aagttctgct
     3301 ctaggcttta tactcgtagt ctttggctct ttgatagcag cagtagtttt aggttaccat
     3361 ttataccgga aagattatac aaattaaaga atctatttaa aacaggtatc aaacaaagaa
     3421 atttgttttg gcaatacaat ttaaaaacta ggaggaatac agcaatgaca gaaccaaaat
     3481 taatcgaccc gcgaaacttg taccatacgg agaaatttcc taaacaagat caggatacac
     3541 cagctttaca gcagaatatg attcctgttc ctgattgtgg agaagaatcc tatgaaggca
     3601 ataatcaact agaaaaccgc cgtgtactaa tcactggtgg agattcaggg atcggtcgtg
     3661 cagcagcgat tgcatttgct agagaagggg cagatatcgc tcttcaattc ttcccaggag
     3721 aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaaga tgcaggacgt aagattgtct
     3781 tactaccttt tgatcttcgt gacgaacaag cacctaaaga aatcatagat aaagccgtga
     3841 gtgaacttgg cgggctagat acgatggtac taaacgcagg acagcaaatt tcttgtcagt
     3901 ctattgctga gttaccactt gagcaagtca aagacacatt tatgataaac atcatcgcta
     3961 tgtttgcttt agtgaaagaa gctgttccgc atatcccagc aggaggagca atcgtaacaa
     4021 caacttctgt tcaagcattc aatccaagcg aacatttatt agattacgca gcaacaaaag
     4081 cttcaatcgc taactttacg atcggtttag caaaacagct tgcacctaaa ggaatccgtg
     4141 taaatggtgt agcacctggt ccaatctgga caccattaca actagacgaa ggacaacctt
     4201 ctgattcgat ccctgaattt ggtcagcaaa gtttactaga acgtgcaggt cagccaggag
     4261 aattagcacc agtttatgtt ttcttagcat cgaataaagc aagttatgtt acagcccaaa
     4321 tttacggagt aactggtgga gaagcgatca acttataaga agttgatcgt ttcaccccta
     4381 tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg gagtgatcgg aacattactt
     4441 ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa cctatccatt gccttttcag
     4501 ctagagaagt ttcgattctt ttccatcact aattcatacg tgcaacaata tgtattttgg
     4561 cttgccgttg catttgctat tttgacaatt atcacgttac ttgttttcct gttttatcca
     4621 aaaacaatcg gaaactttct actgaaacat ggtgatgggg agttgacgtt ggataaaaaa
     4681 gcaattgaag gaatggttcg ttctcacttg catgaagatg aattcatcca ttcaccaaaa
     4741 gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg taaaagggga gttgaaacga
     4801 acttcagcac tcattggtaa aacaggaaca ttgatgaaag aaatcgaaaa tgaagtacaa
     4861 cacatattgg gaacaaaaga gccagtgaaa gtatcggtga aatacacagg ctttgaagaa
     4921 ccagctaaac gaagcaatga ccgcaaacga gtagaatagg gggggaccaa aaatgaaaga
     4981 actattttcg acatacaagc tccctatcat ctttggggta cttggattga tattagccgt
     5041 tttatggatc accgtgggct ttttcaagac cttattgatc ttgatcatgg cagcgatcgg
     5101 cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta gatcactatt ttaaatctta
     5161 ggaggaaaca tccatgaata acaataacaa aaataataac aataaaatga acaaccaaaa
     5221 accagtgtct gcacatccag acccaacgac accaaaccaa tcaaatggtg tgaacgatga
     5281 aaagaacaaa acagcaccag tatctgcaca tccagatcca gtagtcccta acgttcaaca
     5341 aggagtagaa gagaagaaac atgatgcaga aaagcatgca cctcatcatc caggaactcc
     5401 agaatctaaa gttccagaac aacatgatgc aatcgatcct tctaaacctg tggaagagca
     5461 aaaacatcct tctgaccatg cgaaagcaca agaaaaactt agttctgacc acacaaacgg
     5521 acaaggaaaa cttggttctg atcatacaaa taaccaagga aagcatgatt ctgaacagga
     5581 agattcaatg cgcggtgagc tgaattatga agataaagtt gtgcaaaaaa tcatcggaat
     5641 cgcaattgaa caagtagacg gtttattatc tgcaaacggt ggtttcttct ctaatgtggc
     5701 aggaaaactt gtgaatacag ataatgtaac ggctgggatc gaaacagaag tcggtaaaaa
     5761 acaagtagca gttgatatgg atgtcattgt ggaatatggc aaagatatcg aaaaaatctt
     5821 tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat atgacacatt tagaagtgat
     5881 cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa gaatttgaaa aagatcaaga
     5941 aacggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc ggtgaatttg cttcgaatca
     6001 aacagataaa gtaaaatcag cagctggaag cggcgcacaa aaagtaaaag aaaatacaga
     6061 accacgggtt cagtaataag gaagtaaaac attaggagga acgaaacatg ggatttattt
     6121 ggtcacttat cgtcggagga attatcggag caattgcagg agcaatcacg aataaaggtt
     6181 cttcgatggg gattattgca aatgtcgtag caggtttagt tggatcagca cttggtcaag
     6241 cattattagg ttcttgggga ccaagtattg caggaatggc aatcgtgcca tcattgattg
     6301 gtgccatcat tttagtacta atcgtatcat tctttgctcg aaaactgtaa aaagactttt
     6361 aaaaagaggt cgtgaaaaag ctgttcttca tcaagtaata agaacagcca aacaaaaata
     6421 gcttctcata tttttcattt ggttgggcat attatcgaag atgcaagctt ttgaacactg
     6481 tttatacgga ataagaggct gtgacaagac ttttgtcaca gcctcttatt tatgcgggca
     6541 aattcccaat tagattttta aaagtttttc cttaaatgtg tctttattga tacagagaag
     6601 cttctttaat aaaacaatgc ctccctaaat tttcttgaga taggaaaggt gaacatacaa
     6661 aataatacga ccactttgaa aacccagatg atccacaaat ggatcgtatg atactgatga
     6721 agtaaaaagc aatggatcag aaactcaaag agattgaaaa agacaaggaa gaaataatat
     6781 ctctttttta ctcttgtctg ttttagccaa taatagctcg taaagtaatc tagacaaaca
     6841 aatagaagta aacaaagttt cgtaagagca gcaatcgtat catgttgctc tgctagacga
     6901 ttgacgagca ggaaaaatgt tggtaatgga atatagatca cgagtaatga aagaaaaaag
     6961 taaacaataa atggcatact attcctccta attgtgtgag ttcgccctca ttttaaaata
     7021 acg
//
LOCUS       DAXWLG010000129         6933 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXWLG010000129 DAXWLG010000000
VERSION     DAXWLG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.130"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..564
                     /locus_tag="ACGE4T_002131"
     CDS             <1..564
                     /locus_tag="ACGE4T_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8387371.1"
                     /translation="YSPSEKNTPSDKSTFDGDKSFPLPGEKFLQELNHSSKDFKDFKE
                     SQNADFIDSLSVENKKLDEILIESLIEKEWLYQVWGDNIVNCLKLVGNNDYSQFYDWY
                     KKFKYAIKSAEKEQKIQLKIIYEICNNKYFVDYVREQLSMTLRDLIRRAKTDHRIKQK
                     DDYIFASLKNKALELIELQISEGIDKQ"
     gene            640..858
                     /locus_tag="ACGE4T_002132"
     CDS             640..858
                     /locus_tag="ACGE4T_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387372.1"
                     /translation="MIENRLIDQVWCLDQNTDNLELIETICFNTIVLDLRSENDNCVT
                     HIIIDRKTAQKLSESLRKWAEGGNNENS"
     gene            845..3538
                     /locus_tag="ACGE4T_002133"
     CDS             845..3538
                     /locus_tag="ACGE4T_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFF8387373.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            3552..3749
                     /locus_tag="ACGE4T_002134"
     CDS             3552..3749
                     /locus_tag="ACGE4T_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387374.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            3753..4364
                     /locus_tag="ACGE4T_002135"
     CDS             3753..4364
                     /locus_tag="ACGE4T_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFF8387375.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4349..5740
                     /locus_tag="ACGE4T_002136"
     CDS             4349..5740
                     /locus_tag="ACGE4T_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFF8387376.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5956..6270
                     /locus_tag="ACGE4T_002137"
     CDS             5956..6270
                     /locus_tag="ACGE4T_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387377.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6263..6757
                     /locus_tag="ACGE4T_002138"
     CDS             6263..6757
                     /locus_tag="ACGE4T_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387378.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
BASE COUNT         2632 a          938 c         1358 g         2005 t
ORIGIN      
        1 tacagtccaa gtgaaaaaaa tacaccttcc gacaaatcaa cattcgatgg tgataagtca
       61 ttcccgcttc caggtgaaaa atttttacaa gaacttaatc attcttctaa agattttaaa
      121 gattttaaag aatctcaaaa tgctgacttt attgattcac tttcagttga aaataaaaaa
      181 ttagatgaaa tattaattga atcattaatt gaaaaagaat ggctttatca ggtatggggg
      241 gataatattg taaactgtct gaaattagtg ggtaataacg attacagtca attttatgac
      301 tggtataaaa aattcaaata tgcgattaaa tctgctgaaa aagaacaaaa aattcaactg
      361 aaaattatct atgaaatctg taataacaag tattttgtag attatgttag agaacagcta
      421 tcaatgactt tacgtgactt gatacgaaga gcaaaaacag atcaccgtat caaacaaaaa
      481 gacgattata tctttgcttc tctaaaaaat aaagcgttag aactaattga attgcaaatc
      541 agtgaaggaa tagataagca ataagaatag aattgagtca aatgaagtgg aggggtatct
      601 ccgcttcatt tgagaacttt gaatatagga ggggttattt tgattgaaaa tcgtttaata
      661 gatcaagttt ggtgtctaga tcaaaataca gataatctcg aattaatcga gactatttgc
      721 ttcaacacta tagttctcga tttaagatct gaaaatgaca attgtgtaac tcatatcata
      781 attgacagaa aaacagctca gaagctctct gagagtctca gaaaatgggc tgagggaggt
      841 aataatgaaa atagttaaag aaaagattgc cagtagaaaa atacactggt tagctttgag
      901 catacttgtt ttaatgttta tcgttgtttt caccggtaat gttgtccaag caagcatttt
      961 caattggttt gatgacacgg aaagtcaaac tgaatttttg aataatcctg tctatcaatc
     1021 gtatttaaaa aaatataacg gaaattttta cagtcagttt ggattgtgga tagggtggtc
     1081 tgttataaaa tcgatgtttg caattgtaga tggtattcaa aatatgattc ccgaagtgtt
     1141 agatttgttt agctttattg aaagtacggg aatgaataaa gtttatcaat cggtcttaag
     1201 tacagttgtt attggtttaa tgatactttc actgatgttt gttggttaca aaatgattac
     1261 tggaaaagga acagtggatt taaaatcggt tggaatgaac gtagtgatgt ctgtagcatt
     1321 aattctttta atgccgactt tgattggatc aacagaaaaa ctttcaggaa taatgtttgc
     1381 aaagactctt tataatgacg caactagtat caataatcaa gatggcggtg tagcatggtc
     1441 attgattgag cagggagtga cagatttagc ttatattaat aagacagatc aatattcgac
     1501 aattgataaa acaaatgacc gaaataatct aacaaaagaa aatttctgga aaacggatct
     1561 tacgcaagta ttgacagata atgtaattga taaaatggaa aaagagaacc caacagctga
     1621 taatctgcgt tatgaattag ttgaaaattc tgataatgaa tttgttgcta ctaaattttc
     1681 agataatttt ttatcaacct ttgttgatgg tatcaaatca ggatattatc gctatcaagc
     1741 gaacctgtgg ggaatttctt ttggattgat tgctttagcg gtagcgtatg tttttagtgc
     1801 tttcattata attacagcaa tactcgagtt agcatttaaa cgtgtactcg gagttttagt
     1861 atttgctact gacttagaaa caggacaacg atcaaaacaa gttttgtcag atattttaca
     1921 gtgctatttg acagtcggat ttcaagggtt cggtttatct atgtttgcaa tgttcataaa
     1981 ttacctaaat actggcgaag gtatctcagc aaatattttt ataaaaacaa ttgcttatat
     2041 ctgtgctgtg tttgttttaa tcaaaggcag tggaacagtc atgagatact ttggtgtgga
     2101 tattggactc aaagaaggct atgggcaact tgcttctgct tttggaatgg gtgcaatgct
     2161 attccgtaaa ggaacaggtg gtatgaaccg gtctaaaggt gggaaaaata atggtggaaa
     2221 cgatcaagct gaaggggaag aacgaaaacc tgaacgcaac tttggacaat cactttctaa
     2281 aaaagctaat aaaacgggga aagcgcttgg gtatgctcat gaacgtggat tatcaggttt
     2341 agcttcagat ggatctacaa tggctagtga aagagcaaca aaaccattta agagtatgcg
     2401 tgatatagct aatgatacca agagtaagtt taaagaaggt ttagatgatg gtactgtgtc
     2461 cgctattaat aaaaatagca aacctatgtt agctaaaaat aaagaagatg aaactggaaa
     2521 atatgctgat tccatgccaa cacgtctttc ggatagaaaa gatggtgcta aggttgaaaa
     2581 tgccgatagt attatgtcta gttctgaacg tatgcgtgaa gcaatgaaaa ataatgctga
     2641 atcgaataat aatcctgtaa gtgcaattca gcaaaaagtc caacaagata ttgaagaacg
     2701 taaaaatgct atgcacggtc aagctaagtc tgctgaagaa ttaattaatc agaagcgtca
     2761 tgaagcgaaa tacacgcctg aagcaatgaa tcgcgaggaa ataatgttaa ggaaacgtgt
     2821 agaagggcgg acaggcgcta atattgacgg aaaagaagcg ttgaccaaag aaagaattca
     2881 agaagctaaa aattcaaata caggattaga aaaattagtc aaagaaaatc cacaaaattc
     2941 tacaagtagt catggtggcc gatcagtaga tgtccgagaa aatattcaag gttcactgaa
     3001 tggtcatgtt ggacggaatg ttgatgtaag agagaattta caaagttctt caaatgctca
     3061 aggtactaag acaattgatg ttcgagaaaa tctgcaaagt agcagtacaa gtcaaccaaa
     3121 aacagtagat gttcgagaaa atgtacaaaa ttctacaagt agtcaagggt ttaaatcagt
     3181 ggatgtccga gagaatgtgc aaggtagcag tgcaagtcaa ccaaaaacag tagatgttcg
     3241 agaaaatgta caaagttcta caagtagtca aggattcaaa acagtagatg ttcgggagaa
     3301 cgttcaaaaa gatagtggag aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga
     3361 cagaaagtca gcgaaaaaat ttgattcaaa tcatgaaaca gtgattattg atagtgaaat
     3421 ccgtgaaaat gataagggat caaaaccaag acgaaggttt acctatgaag acaacgagtt
     3481 attcagagat acacttaatg atcctaatcc acttttcgat agtttactta aaaaataaag
     3541 aggtgattgt catgcaatta attctacaga ttagtacaca tgatttctta gaacttgacg
     3601 aattatgtga atcagtaaag aaagcaacaa atgaaaaaat cacggtcact aattatattg
     3661 aactgatgat tcaagaagaa ttaaagatga aaaatgaaat agagagcgat ttcaaatgtt
     3721 gctctctaga aaaaaatgtg aggggataat caatggatga taaacaaaaa atgtacgaaa
     3781 aaaatcgcaa acatagtgag gccttaaatt ttcgtgtcga taaaaagact tatcttgaac
     3841 tgacattgat tgcaaaacaa aggggaattt ctagagcaga cgtttgccgt gagcggttaa
     3901 agggactcaa atatgaaccg ccgttgttag ctgaagatga tagggtagtt ttaaccaaac
     3961 agttaaatca aattggtaac aatttgaatc aagcggtaaa ggcattaaat atcattgcta
     4021 aatattttag agaagaatct gaagataaaa aagaaaagag cctacatttt agttttgatg
     4081 ctagccgtta caacattttt aaagatgaac ttgcgaatag catagcaaaa aagttgatga
     4141 ttagttatcc agatgacatt tttttggagt ccaatcggag tgccccctct ttttttaaat
     4201 tatcagaagt agaaaaagaa tatttcgaat attggataga aaagcaaact agaccgggta
     4261 cgaaagaaga gtattcccaa aaagttgatc ggaatttaat tcactcaatt cagattattg
     4321 caagtttaaa agatgaggtg aaacaacttt gggagctact ataacatcga aaggacgcaa
     4381 gaaaaattta tcctcgctat tacaatatgc agctaatcct tcaaaaaaag aaaaatgtgt
     4441 tgcagtctca ggaataaatt gtagtgatga tagtgaggaa gcactgcaag aaatgaattt
     4501 agtacgtacc aagatgaata aatctaaaga tatagcacaa gcgtttcatg ttattcattc
     4561 gtttgataag agtacttcta aagagttaag tattgataaa atgcacgaaa tagctgtcga
     4621 atttgcagag aaagcttttc caaattcgca aatcattgtt gcaagtcaca atgataaaga
     4681 tcattttcat agtcatcttg tcattaataa tattaacatg gaaacatgta aaaggttgcg
     4741 tattgaccct aaagatttag aactaatgcg aaaaattaac gatcaagtcc tcaatgaaca
     4801 tggtcttgaa tcagtgaata atgagtacta tgaaaactta gaaaagagaa ccaatcttta
     4861 ttcaaacgat ccttctaaga tgaaatatgg aaaaattaat tatcaagagt ttgtgcaaaa
     4921 tgcgatcgaa gcaacgttgg aaaaatgcga agtgaattct tttgaagatt tttcggatca
     4981 tttggcaaaa gaatataaca ttgaagttta tcaattttca aaaacaaata acaaacttgg
     5041 ctatgttctt tacaaacaag gtgtaaattt ctatgagcgt agaggtgagt taattacttt
     5101 aggaagagat aaacaatcta gaaaagaaaa aagtcagtat gtcgaacgta cttttagtgc
     5161 aagaaagtta gggaaaagat attcatttga atctgtttct gaacaacttg aaaagaattt
     5221 agaagaatca catataaaaa atttttttaa tatggaaaga gagcaagtta tgtcgctaat
     5281 tagtggagta aattacaaaa ctggtgagtt aaatgtacct tttgtggaat tagattccat
     5341 cgcacaaaag caatttagcg accgtgaagg cgatgaaaaa ttcattaaat atgatgactt
     5401 cttgcgagag gtagacaagc attttgtaaa cacaatgtct ttagaagatg atcaagaata
     5461 cacgattaaa cttcatacgg atgtgaatcc tcaaacttta cgtagtgcga aaagtaaaac
     5521 aatttctatt aaaaagactg atcagaaaga gttatttcta ccggcaaagg gttttgaaaa
     5581 ttgttctcat acaccttctg ctaaacagat tcactatcaa atcagtcgca taatgaatga
     5641 cgatgtaaaa atgtatcgtg ctaaaaagga ttatgaattc acacaacaaa tgattgaaaa
     5701 tcagcaaaaa aggcaacaaa attttgaaat ggaattctga atgggcttgt tagtggactt
     5761 tccagttttt tgcctatgaa gagttgcttg gaaagaagca gtttttagga catacgccag
     5821 ctacccatct acttgccagt caatccgccc cgctcacgct aggctacttt ctggcaagtc
     5881 agatggtggg taaatctctg agggagcaag ctccctacag actccctccc aaaaaaagaa
     5941 aggtggaaga aaaaaatgaa ctatccatat tttaaagtct cggcttcaga agaaactaaa
     6001 gaaatattca acaattttta caaccaaaac aaaggagttt ttggttccaa ggcaaatatg
     6061 tttcgtgtta tggtttcaaa tttaccagtg ttagctagtc catcaaataa taaatttaat
     6121 gattctgaaa gcattaaatt tgaacaaaaa atttcagaac tagaatcaat gataagtaat
     6181 gaagtaattg aaaagcttga tgacattgat caaaagttat cgtattctct aaaaaacaaa
     6241 tacaaaacgg aggagaaaaa agatgtttaa agtttacttc ccgtttcagt ggctcgttaa
     6301 aggaattctt ttagtgctac taggattgat tctttttgga agttatctaa caattaaagc
     6361 cagacatttt ccagaagaaa agccaataaa aattgtaaca atagatttaa aaataggtga
     6421 aacttatcag gcagatgggg ttgaactaac atttaaaaat attgaagtga ccgaaaaaag
     6481 aaatgaattt gcacaacatg atcgtgtatt acaagtgacc tatacactta aaaatgatac
     6541 agaaaaacca atacgtccac aacaatggtc gattgaaaat aaggatggtg aagagcttta
     6601 tgagtatgct atgatattag ataatccaga gtatattgaa agtgacaaag aagaaacagt
     6661 aaattactat tatgaattac cgattgaaag gcaggactac caaatcgtag tacgtagagc
     6721 gaatggttat tttgttggat taccctttac tttgtaagtc ttattttgtt agtggctttt
     6781 aagttaatat catttttgtg atttttgaaa atgaaagtga ggaaattgga agaatggctg
     6841 aagaaaataa agttgttccg tttgaaacat tagtgataaa taaggaagag tttgagcaac
     6901 tttttcttga tttttttatt caatatgaca gga
//
LOCUS       DAXWLG010000130         6813 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXWLG010000130 DAXWLG010000000
VERSION     DAXWLG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.131"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(139..597)
                     /locus_tag="ACGE4T_002139"
     CDS             complement(139..597)
                     /locus_tag="ACGE4T_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8387379.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(610..1941)
                     /locus_tag="ACGE4T_002140"
     CDS             complement(610..1941)
                     /locus_tag="ACGE4T_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFF8387380.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1952..3394)
                     /locus_tag="ACGE4T_002141"
     CDS             complement(1952..3394)
                     /locus_tag="ACGE4T_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8387381.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3381..4145)
                     /locus_tag="ACGE4T_002142"
     CDS             complement(3381..4145)
                     /locus_tag="ACGE4T_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFF8387382.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4249..5124)
                     /locus_tag="ACGE4T_002143"
     CDS             complement(4249..5124)
                     /locus_tag="ACGE4T_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8387383.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5379..5463)
                     /locus_tag="ACGE4T_002144"
     tRNA            complement(5379..5463)
                     /locus_tag="ACGE4T_002144"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5427..5429),aa:Leu,seq:caa)
     gene            complement(5481..5552)
                     /locus_tag="ACGE4T_002145"
     tRNA            complement(5481..5552)
                     /locus_tag="ACGE4T_002145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5518..5520),aa:Gln,seq:ttg)
     gene            complement(5562..5634)
                     /locus_tag="ACGE4T_002146"
     tRNA            complement(5562..5634)
                     /locus_tag="ACGE4T_002146"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5599..5601),aa:His,seq:gtg)
     gene            complement(5696..5780)
                     /locus_tag="ACGE4T_002147"
     tRNA            complement(5696..5780)
                     /locus_tag="ACGE4T_002147"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5744..5746),aa:Leu,seq:caa)
     gene            complement(5801..5871)
                     /locus_tag="ACGE4T_002148"
     tRNA            complement(5801..5871)
                     /locus_tag="ACGE4T_002148"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5837..5839),aa:Cys,seq:gca)
     gene            complement(5917..5988)
                     /locus_tag="ACGE4T_002149"
     tRNA            complement(5917..5988)
                     /locus_tag="ACGE4T_002149"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5954..5956),aa:Gln,seq:ttg)
     gene            complement(6000..6072)
                     /locus_tag="ACGE4T_002150"
     tRNA            complement(6000..6072)
                     /locus_tag="ACGE4T_002150"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6037..6039),aa:His,seq:gtg)
     gene            complement(6075..6148)
                     /locus_tag="ACGE4T_002151"
     tRNA            complement(6075..6148)
                     /locus_tag="ACGE4T_002151"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6114..6116),aa:Trp,seq:cca)
     gene            complement(6155..6235)
                     /locus_tag="ACGE4T_002152"
     tRNA            complement(6155..6235)
                     /locus_tag="ACGE4T_002152"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6199..6201),aa:Tyr,seq:gta)
     gene            complement(6237..6312)
                     /locus_tag="ACGE4T_002153"
     tRNA            complement(6237..6312)
                     /locus_tag="ACGE4T_002153"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6277..6279),aa:Phe,seq:gaa)
     gene            complement(6348..6420)
                     /locus_tag="ACGE4T_002154"
     tRNA            complement(6348..6420)
                     /locus_tag="ACGE4T_002154"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6385..6387),aa:Asp,seq:gtc)
     gene            complement(6430..6502)
                     /locus_tag="ACGE4T_002155"
     tRNA            complement(6430..6502)
                     /locus_tag="ACGE4T_002155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6467..6469),aa:Val,seq:tac)
     gene            complement(6518..6589)
                     /locus_tag="ACGE4T_002156"
     tRNA            complement(6518..6589)
                     /locus_tag="ACGE4T_002156"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6554..6556),aa:Glu,seq:ttc)
     gene            complement(6604..6693)
                     /locus_tag="ACGE4T_002157"
     tRNA            complement(6604..6693)
                     /locus_tag="ACGE4T_002157"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6657..6659),aa:Ser,seq:gga)
     gene            complement(6733..6806)
                     /locus_tag="ACGE4T_002158"
     tRNA            complement(6733..6806)
                     /locus_tag="ACGE4T_002158"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6770..6772),aa:Asn,seq:gtt)
BASE COUNT         2065 a         1502 c         1108 g         2138 t
ORIGIN      
        1 aatcatagga gcaacaaaaa aattcgataa ttaacaaaaa tcaaaatgat taaatctagt
       61 gattgaaaca tatcatttca aaaaaatttt gtaaattcac ccataaatat caccctattt
      121 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
      181 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      241 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      301 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      361 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      421 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      481 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      541 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      601 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      661 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      721 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      781 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      841 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      901 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      961 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
     1021 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
     1081 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1141 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1201 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1261 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1321 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1381 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1441 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1501 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1561 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1621 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1681 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1741 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1801 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1861 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1921 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1981 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     2041 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     2101 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2161 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2221 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2281 tgtttctctt attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc
     2341 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2401 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2461 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2521 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2581 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2641 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2701 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2761 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2821 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2881 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2941 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     3001 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     3061 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3121 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3181 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3241 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3301 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3361 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3421 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3481 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3541 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3601 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3661 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3721 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3781 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3841 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3901 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3961 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     4021 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     4081 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4141 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca
     4201 tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct
     4261 tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg
     4321 ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc
     4381 agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag
     4441 gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga
     4501 ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt
     4561 taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc
     4621 atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat
     4681 atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct
     4741 atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt
     4801 tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc
     4861 cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga
     4921 atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga
     4981 aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc
     5041 attatgattc agctttttca tcacatacac cgttaattca cctcctctat atgataaaac
     5101 aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa
     5161 aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca
     5221 taatttacaa aaatagttta acacataata aaagcgttat caatatcttt tttactcgtt
     5281 ttatgataat acttgtatcg aattgatttg gaggtaatta cataaaaaca aaaaaaatat
     5341 agcaactctc tataagaaag ttgctatatt gataattata ccggcggccg gggtcgaacc
     5401 ggcacgtcct caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     5461 ggcaaattta aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
     5521 agtcccttgc cttaccgctt ggctataccc caagaataaa gggcgaatga tgggaatcga
     5581 acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat
     5641 aaaagatgtt atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg
     5701 gcggccgggg tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct
     5761 gccaattccg ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa
     5821 ccggtgatga aggttttgca gacctctgcc ttaccacttg gctataccgc cattatacta
     5881 ttatcaatct ataaaataaa gaataaatta tagaaactgg ggtagctgga ttcgaaccaa
     5941 cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaaaagg
     6001 gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt
     6061 cgccacattc gccatggcag gggcagcagg aattgaaccc acactaacgg ttttggagac
     6121 cgctgttcta cctttaaact atgcccctat aaaatggagg agagtggatt cgaaccactg
     6181 aaccctaagg aacggattta cagtccgtcg cgtttagcca cttcgctact cctccatggt
     6241 ggcgcgggac agaatcgaac tgccgacaca cggagcttca atccgttgct ctaccaactg
     6301 agctaccgcg ccatatttta aaattattaa ttgaataaca agaacgacgg ttccgacggg
     6361 aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt aacccctaca ccacggaacc
     6421 agttcattat ggaggttaac gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct
     6481 ctcccagctg agctaaacct ccaacattca attaaaatga cccgtacggg attcgaaccc
     6541 gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caacgggcca ggtaaatttg
     6601 ctacggagag taagggattc gaacccttga gacagtttgg caccatctac atgatttcca
     6661 atcatgctcc ttcggcctct cggacaactc tccagagtga aagaagctca atactctttc
     6721 tcaaaatgag aactccggca gtaggactcg aacctacgac atcatgatta acagtcatgc
     6781 gctactacca actgagctat gccggaataa cat
//
LOCUS       DAXWLG010000131         6767 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXWLG010000131 DAXWLG010000000
VERSION     DAXWLG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.132"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..890
                     /locus_tag="ACGE4T_002159"
     CDS             <1..890
                     /locus_tag="ACGE4T_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8387384.1"
                     /translation="QLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGVS
                     SAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIGH
                     YTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTED
                     TLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYPD
                     NLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCTL
                     FGKGFGNYDQDIKLKVYYS"
     gene            complement(963..1787)
                     /gene="nadE"
                     /locus_tag="ACGE4T_002160"
     CDS             complement(963..1787)
                     /gene="nadE"
                     /locus_tag="ACGE4T_002160"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFF8387385.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1799..3271)
                     /locus_tag="ACGE4T_002161"
     CDS             complement(1799..3271)
                     /locus_tag="ACGE4T_002161"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFF8387386.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3908..4270)
                     /locus_tag="ACGE4T_002162"
     CDS             complement(3908..4270)
                     /locus_tag="ACGE4T_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8387387.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4493..5245
                     /locus_tag="ACGE4T_002163"
     CDS             4493..5245
                     /locus_tag="ACGE4T_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8387388.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5385..6308)
                     /locus_tag="ACGE4T_002164"
     CDS             complement(5385..6308)
                     /locus_tag="ACGE4T_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8387389.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6323..6595)
                     /locus_tag="ACGE4T_002165"
     CDS             complement(6323..6595)
                     /locus_tag="ACGE4T_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387390.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2064 a         1349 c         1157 g         2197 t
ORIGIN      
        1 tgcaactaca aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa
       61 ggatcaagat catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag
      121 aacttggagt gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca
      181 tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg
      241 atatcagttt tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta
      301 ttggtcacta tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt
      361 ttatcgggaa agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca
      421 ttctctggtt taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg
      481 atacattgga gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg
      541 acaattggcc ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga
      601 gccctggaga ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc
      661 ttaatcaata cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg
      721 gagaaccctt gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt
      781 tgcgaatcga agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag
      841 gattcggtaa ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt
      901 ttctatttta taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat
      961 tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg
     1021 ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa
     1081 tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca
     1141 tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga
     1201 agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca
     1261 tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg
     1321 gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata
     1381 tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca
     1441 tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca
     1501 gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga
     1561 tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct
     1621 gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat
     1681 tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt
     1741 gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct
     1801 atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat
     1861 ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat
     1921 actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag
     1981 gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa
     2041 agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt
     2101 cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat
     2161 ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg
     2221 ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata
     2281 ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat
     2341 caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag
     2401 catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata
     2461 tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt
     2521 ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta
     2581 agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat
     2641 aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag
     2701 cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg
     2761 ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga
     2821 cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca
     2881 gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatt aatggttcat
     2941 ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt
     3001 gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg
     3061 aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg
     3121 cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag
     3181 ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg
     3241 tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca
     3301 tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa
     3361 aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc
     3421 tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag
     3481 acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag attttcctac
     3541 tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac
     3601 ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc
     3661 ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca
     3721 agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac
     3781 aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct
     3841 cgccttgttt tttattgtat acattagata tggagcaggt taccataaag atgattatta
     3901 gtcgttatta aatagaatcc ttcgtatttt tttgttctat cactacaata tctttatttg
     3961 gacgtttgaa ttgtattccg gcacgttcta gctcctgttt cactcctaca tggactttga
     4021 catcttctcg tgtcattttt aaaacgtgat gaatcgcata gtcgatttct tgccttgtga
     4081 tttttttctt cgtattgatc atcacgactt gctttttatc atatgtgtct accaatagca
     4141 gttgtgttcg atctaacgaa aataatttgt aataattaat agccgtattc ccaaaaaaga
     4201 attccactac tttaggataa aatgtcttaa tatttaaatt actattcaca ggtgtttcga
     4261 ttagcttcat ctcattcctc ttttctatat agtcgattta gatccaatct taagttatgt
     4321 taccatcttt cctagcatta aggaatcggt tacaattgaa cctttaaaga caaaaggatt
     4381 taccttttat cacttaatag tataacataa tttcgtgaga aaaaaataca attttttctt
     4441 gaccatctcg ttacgtaaag gattatgctt acatttgtca ggaggaaaaa taatggagta
     4501 cacaataaag aaaatgtcag agatatcagg agtcagtccg agaaccttgc gcttttatga
     4561 tgagattgga ctgctaaaac cagctaggat caattcttca ggttatcgga tttatggcaa
     4621 aaaagaagtc gatcgtctgc aacacatctt attttatcga acaatggcat tcaagttgga
     4681 tgatatccaa gaggtcttag acaatccatc ttttgaccac cagaaagctt taatcaagca
     4741 tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa ctgtccaaca
     4801 gacacttgat atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat ttgaagggtt
     4861 taaacaacaa aagttgaaag aaaatgaaga atcctatgga caagagattc gacaaaaata
     4921 cggaaaaaaa aaggttacag catccaatga aaaatggatg aatatggata aagaaacgta
     4981 tgaagaaatg caaactgttg aaaagcagat gttatttgat ctcactcttt acttaaaaga
     5041 accagaagat gaaagcttag cagaccgtat cttccaagct cataagaagt ggttctctta
     5101 cagctggcct gaatatcagc caaaagcgca taaaggacta ggaatgatgt acgaagccga
     5161 tgaacggttc actgcatatt atgacgagcg aagtggtcat ggtgcagcaa aagctttaaa
     5221 tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag tacacgtaaa
     5281 atgacctaat aacaaaacag cagacaccca agttagattt ttatgctaac ttttggtgtc
     5341 tgctccattc tttcacaagt tttttcatcc tactttaaat atattcatcg ttgaataaac
     5401 gtataaggta ttttcacttt atcctttttt tctgtaagaa acaattgaaa tgccatttct
     5461 ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct gatcagcaaa
     5521 ttatcgcgtc caatcaccac tggtgtctta tcttgcagat agctttgcaa gatgcctgct
     5581 gcaaccatat ccgaatttgt aaagacacca tcaactccaa gctctttaaa aaagaagcct
     5641 gcttgttttc ctttctctgc atctatacaa tcccaaaaaa caagtgattc atcaaaagat
     5701 ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt tataacttag cctgccactt
     5761 cgccccaacg tgatgcccaa ttgctttata ccttgctgct tcaaataagt aagaccttct
     5821 gtaattgatt gttctcgatc aataaagaca cagcttgcct gttttccctt gatttcttca
     5881 caaaaaacta gaggtccata tttttgatat gccaataaat catctagtgt attggcacga
     5941 gacgtaatga tcaatccatc gaaagcttta gctgcaaatt cttcaagata ccttcgctct
     6001 ttttctttat gatagcccgt cggtaaaagt gtcactttgt aatcataatc aaacgctgct
     6061 tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat tggtataagg cagaatcact
     6121 ccaatgcttt tcgtttttcc ataacttaac tgtctggcat ttgcattagg gatataatcg
     6181 acttctttca tgaccgcaag tatttctttc cttttttcct catctacata tggatgtcca
     6241 ttgattacac gtgaaatagt agaaactgaa taacctgtga gctttgcgat atctcgaatg
     6301 tttgccactg ctgcttcgcc acctatacaa aaagattttc ttgtaaatag tctaccacag
     6361 ctggaccgca ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg atccgctgcc
     6421 cgccccaata tatatctttt gtcaaatttt cgatttcatt cattggtatt tcttttacta
     6481 tatttctatt caacccttga aaactgtaca acgttaatac ggatcccttg aactcgacaa
     6541 acgcataaaa ataattgttc gttgtgccat ctgttatctc tacataagta ttcattgcaa
     6601 gccctccttt tgatactttg agtatagtgt atgaaacgcg tctcatagca aggagtttgt
     6661 tttgccaaag aaaagtttta ttcctctttc attattacca aagtctctta ttttaaattg
     6721 aatacattat aatgatactg taaatatatc tttattatta gtgaatg
//
LOCUS       DAXWLG010000132         6732 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXWLG010000132 DAXWLG010000000
VERSION     DAXWLG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.133"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            483..1643
                     /locus_tag="ACGE4T_002166"
     CDS             483..1643
                     /locus_tag="ACGE4T_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8387391.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1659..2102
                     /locus_tag="ACGE4T_002167"
     CDS             1659..2102
                     /locus_tag="ACGE4T_002167"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8387392.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(2585..3115)
                     /locus_tag="ACGE4T_002168"
     CDS             complement(2585..3115)
                     /locus_tag="ACGE4T_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFF8387393.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            3220..3771
                     /locus_tag="ACGE4T_002169"
     CDS             3220..3771
                     /locus_tag="ACGE4T_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8387394.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(3924..4397)
                     /locus_tag="ACGE4T_002170"
     CDS             complement(3924..4397)
                     /locus_tag="ACGE4T_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8387395.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            4535..6007
                     /locus_tag="ACGE4T_002171"
     CDS             4535..6007
                     /locus_tag="ACGE4T_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8387396.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            6004..6714
                     /locus_tag="ACGE4T_002172"
     CDS             6004..6714
                     /locus_tag="ACGE4T_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFF8387397.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         2182 a         1189 c         1299 g         2062 t
ORIGIN      
        1 gaaattagaa aattcatgaa aatatttatg aattttacaa gaaaaacata agtgaaagtt
       61 ctaaggagag aggatgcgac ataagttttg ttgcatctcc ttgttttgaa acagaatttt
      121 tcttaatact cgaaacaaag tagctttttc atgacctctc ggtaaacggt gttcaatcag
      181 ctgcttctcg gtaataagct cactagccta gaaaatatga gaagctattt tctgactagc
      241 gctcttatta ctcaaagcat aacgcagttt tcacaacctc ttccttaaac ggtgttcaaa
      301 agcttgcatc tgcggtaata agcccaaccc aacaaaaaat atgagtagct atttttttgg
      361 gctgtactta ttacttgatg caggacagct ttttcacaac ctcatcggct ttcattttct
      421 atttttttcc tgaacccata taataaaaga aagaacaaaa aaacttgcaa gggaggacac
      481 atgtgaagca gagtaagcaa aaacagtctc gtcgactttt gtttcgtttg tttttattaa
      541 tatgtatcgt cgtagggtta ctgatcgctt tatatccatt ttatgtggat tctttaaata
      601 gttttattga tcaaaaacgt atcgaacaag tacaaaaacg gaccgcagct gaaattgaag
      661 tgcagcgaaa gaaaatggag gagaaaaacc aacgattaac tgaccaagga ttcaatcctg
      721 gtactgatcc tttcaatgaa aagaacagaa atgaaagtac aacatccagc caattagagg
      781 aatggctgat cggaagtatc aatataccta agatccaatt aaatatttca ctttatgatc
      841 ggctcaatgg taggatcctt gaaaatggag caggcgtctt gcaaggaacc tctttcccgc
      901 ttggtggaaa ttcgacgcat tcagtgatct ctgctcacag cggtttacct aatcgacggt
      961 tatttaccga attagatcgt ttagaaaacg gagacacgtt tattttgact gttttggggg
     1021 agaaattggc ttatcaagtt aaaaatatac aagtggtact gccagatgac acgtctgttc
     1081 taacgatttc agaaggagaa gacttagtta ccttgcttac atgtacgccg tatatgatca
     1141 acacgcatcg tttgctagtg acaggacatc ggattcctta taacgaatcg gtaaaaaaag
     1201 aagaaggaaa aggaaatcag gaaagaacca tgcggcaatg gtttattctt gcaggaacga
     1261 taatagctgt agtcatactg ttagtgttta tcggacgact aatctatcag tatagattaa
     1321 gtaaaaaagt gcttgatttt tcatttatta ttagtgattc gtcagggaaa ccggtaaatg
     1381 gtagttcatt cattttgaaa cataagaaaa aaacacttac tagaaatggt gtgccgtttt
     1441 cagtacaaag cgaccatcat gggaaagtca agctggatca attgcctggt ggaacttatc
     1501 gaatcgtttc agctgatgat cctaaagttg ctgtttcttt tggtattaga aaagtaaaac
     1561 aagagaagat atactttttt gaggggcgaa aacttgtaaa agagcttcaa aaaaatggtt
     1621 tttattttaa aatcaatgaa taagtaagga gagaaaaaat gatccgcaca acaacaaaat
     1681 tgtctgccaa agaactggaa tctgttctat cgatttggct gcatgctaac ttggattctc
     1741 atgaattcat tccatcaact tattgggaga atcaagtaga atctgttagg aaagctttac
     1801 cagatgcagt gatttactta gcagaaatag atcaagaaat tattggtttt gctggggtga
     1861 ttgatcagtt tatcgcagga atctttgtga aaagggaata ccggcaacaa agaatcggta
     1921 cgcaactaat gaaaaaaata aaagaaacca ataaaacact gactttgaca gtctatcaaa
     1981 aaaatcaatc agctattcat ttctatcttt cacaaggttt tacaattcaa acacagcaaa
     2041 aagatgaagc agtaaatgag gaggaacttg tgatgaaatg gaatcaaaac gctgattttt
     2101 gatgaacaga ataacgaaat aatgctccca gcctaatttt ttgctgggag cattatttca
     2161 ttttgaagtc actaatttaa ctgtgtgaat atgcagttgt tacaatcttt gataaacggt
     2221 gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
     2281 tccatttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tgataaacgg
     2341 tgttcaaacg gctgcttctc ggtaataagc ccactagcct agaaaatatg aaaagctatt
     2401 ttctaactag cgttcttatt actcaaagca taacgctgat ttcacaacct cttgataaac
     2461 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaacat gaggaactat
     2521 ttttccgttt ctttctctaa tactcaggtc agaacgctga tttcacaacc tctctttcac
     2581 aaccttattt tactcgttta taattttcat cgaagtattt tccgctacgg atatcatctg
     2641 gcattccatc gtatggttct tcgctaataa catcttcatt gtctatacct gcatcaccca
     2701 tataagtgat cgtagcaaat tcaggaacaa gtaattttgg ataagtttca tatttttcac
     2761 gagctgcttc catttctgcg atatgttcgt tttggaaagt aacagtgatt tctggatgtt
     2821 cttcaaccca tttagggaaa aagatcattc cgtgaagttt cttttcattt ggcataaagt
     2881 ccaatgccac gtctgtccct gttggctctg tccaagtgat tttgaatacg ccgtccgtca
     2941 atttaacgat atcagcttct tggtctttta cccaacggcc tttgaccatt ccgccatgga
     3001 tacggtaatc aacagtatgg tcatttttgg cataccattc atattcccaa ccattgtcat
     3061 acgtatagat aaagtgagtt ccaataaaat cttccaattc tttaaattct ttcatgttta
     3121 agcatctcct tataaattaa aacatgtaat aaactacatg ttttcgatta ctcatttatg
     3181 atagaatgga tttaaagatt tgtcaaggag ggaaaactga tggcaagaaa aaatacactt
     3241 cgatatattt tgttagggtt attaagtaag aagaagatga caggatatga attgaatcaa
     3301 tcgttcaaaa atgaaatagg agaattttgg caagcaaaac atagtcagat ttatcctgaa
     3361 cttgcaaaaa tggaagagca aggaatcatt cagcatcaag tagagatcac aggtgaaaaa
     3421 ttggaaaaga aagtttatga attaacagaa gaagggaagg aacaattggc agagtggatt
     3481 catacaccca ccaatgaact accggttaat cgagacgaat ttgttctgaa attgtatttc
     3541 gtcaaagacg tgaatgatcc ggcattagta gaaattattc agcagcagcg tgaccttcat
     3601 gaagaaaaac gacttcatct attagagcga aaaaaaacga tctttcctac tgaaaaagaa
     3661 aaacatgaaa attacggaca ctacctgatt ttagcgcatg cgatcaatcg ggaaacagaa
     3721 tatgcaaatt ggctaacaga tgtattagaa gaaagtaacc gaaaaaaata aaaaaatatc
     3781 cgaacagtga tggagaattc ctttctatat gaaagaaacg cagatcaccg ttcggatatt
     3841 cttatgttca aacaggcctt ttttcaagta tttatgtagc atctacttat taaaaagctt
     3901 ggtacttagg agattagtgg cgtttagagt tcttttaaca actgttcgta gtcagctttt
     3961 tcatcgacag gaaggagctt gtaagttaat tcgtctgtct cgttgtcatg aatgatgtac
     4021 atcttagcat tctcttcttt gaagttaccg atttgtaaaa tcgtatagcg ttcgtcgatc
     4081 aattcgatat ctttgattgc ttttgaaaaa actttacgga catggtgcat tttcttttgg
     4141 cggatttctg ccttttctgc agcttttgac aaagtaaatc cgtcatctaa atattttttg
     4201 atcagttcta cttttatgac tgtaggtaaa cgatattttc taggacaatt cgcatcagct
     4261 tcgatcgaac gaatgaatcc cttttgttcc caatacctta attgacgtgg agacacacct
     4321 acgatatcac ttaattcact gatgccaaca accaattgat cattttccaa caaacgttta
     4381 agggaagccc caaccatttt tgtcactcct tttgtcctct tgcttccctt aattatatcc
     4441 gtattaactt atgtgtcaag taggttgaca aatagcgaat ttaggactaa agtattctag
     4501 tagtttcaga aaaaattcag aaagaaggag aaatatgagt aacaaccaac ccgtagatat
     4561 ttacggaaaa ccatataatc ggacactttt agttgttgtc ttattaattg gtacattttg
     4621 taccgttttg aatcagacct tattgacgac agcttttcct acattgatga aagctttcga
     4681 tatttcagcg tctgatgtgc aatggttaac gactggtttc ttgctagtca acgggatcat
     4741 gatcccaata acagcatggc tgatcaataa attcagttca agaaatttat atctttctgc
     4801 catgacgatc tttttgatcg gaacgattac ttgctttaca gcacctaatt ttagtacatt
     4861 actaatcggt cgtttgatcc aagcttgcgg tgtgggagta tcgatgccat tattgcaaaa
     4921 tatcatgcta tctattttcc cgccagaaaa aagaggatca gcaatgggga tgtcagggat
     4981 cgttattgga cttgctcctg ctttaggccc tacattatct ggttatatca ttgataacta
     5041 ccattggcgt gacttgtttg gtatggtact gccaatcgtt gttcttgtct tggttttagc
     5101 attcttccta atgaaaagtg ttattccatt gtcaaatcct agcattgata ttttatccgc
     5161 tattttatca acgattggat tcggtagttt actttacgga ttttctagtg taggagatga
     5221 cggctgggga agcttgaaag taattggttt cttagttgtt ggtgtgattt tcatcgcctt
     5281 gttctcatgg cgtcaattac atttggaaca tccattcttg gaacttcgtg tattcaaatc
     5341 atctacattc acgattgcag ctgttttagc tggtgtaaca aatatggcga tggtaggggc
     5401 agaaatggtc gtaccattgt atattcaaaa tattcgtgga gaatcagcat tccattctgg
     5461 cttgatgttg ttgccgggtg cattgatcat gggggctatg atgcctgtga cgggtgcgat
     5521 tttcgataaa tacggcgcga aacgtttagc catcacaggg atgttcattt tgacagcggc
     5581 aactgcacca tttgctttct tgacaaaaac gactccagtt ttatatatcg tgatccttta
     5641 tgcgatccgt atgtttggga tctccatggt catgatgccg gtaacgactt ctggtatgaa
     5701 tgcattgcca aataacttga tcagtcatgg gacagcggta aataatacat tccgtcaaat
     5761 cgcaagctcg attggtacag ctattttgat cagtgtgttg acgaatgtga caaaagcaaa
     5821 tatcccaagc aaatctgtat tggataatac gccattagct tataaagatc aggcaatcaa
     5881 tgccacactg tcaggttacc atgccgcgtt tatcgtagcg atcatctttg ggattcttgg
     5941 ctttattatt gcctttttat tgaagaaaaa agaacctgtt ctaatgaaag cgggggataa
     6001 tgcatgatta ttggagtttt gattgtcagc ttgcttgctt ttgctttaac gaacgtgttc
     6061 gctaaaaaaa catggcaaac ctttttatct ttgattttcg gactaatctt cgtcgcttca
     6121 ctaagtttga tcgtcgctaa tttgtccaat cactttggaa tggaaaaagt aacggaaaca
     6181 aaaacagaaa aaattgtttc cagtgctgat tcccaaggag cagatatgct tttgtataaa
     6241 gctttaggga atggaaaaga aaaagtgtat ctttaccgaa caaatgaaaa acaagaaaaa
     6301 ccaaaagcaa ctggtacgga taacgaaaca aataaagtag aaaagacaga cggcgatgct
     6361 gaaaaagtga cgaaaactac ttattgggaa tacaaaaacg acatgtataa attctggttc
     6421 aatattgctg ataacaatca cgaatatgac agccgagtga atacattcaa aatcccagag
     6481 acatgggtag aattaagtac ggaccaagca gccaagttag ctgaattagt gaagaaacag
     6541 caaagtacga tggaaagtga agcaaaagct tatgttcaag acggtatggt aaaagcaatg
     6601 actgaaaatc caaaaatgag caaagcagag caagaacaac gtacgaaagc cttagctgct
     6661 gaattccaac aacaagcatt cgcaaaatta gtgaaagaag caaaaggcga ataatatttt
     6721 gaacaccatg ta
//
LOCUS       DAXWLG010000133         6730 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXWLG010000133 DAXWLG010000000
VERSION     DAXWLG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.134"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..507
                     /locus_tag="ACGE4T_002173"
     CDS             <1..507
                     /locus_tag="ACGE4T_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387398.1"
                     /translation="GFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLLLNK
                     FTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSI
                     SLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNN
                     FYFYRNKL"
     gene            complement(633..1310)
                     /gene="nth"
                     /locus_tag="ACGE4T_002174"
     CDS             complement(633..1310)
                     /gene="nth"
                     /locus_tag="ACGE4T_002174"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFF8387399.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1317..2015)
                     /locus_tag="ACGE4T_002175"
     CDS             complement(1317..2015)
                     /locus_tag="ACGE4T_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8387400.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2283..3467
                     /locus_tag="ACGE4T_002176"
     CDS             2283..3467
                     /locus_tag="ACGE4T_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8387401.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3473..4039)
                     /locus_tag="ACGE4T_002177"
     CDS             complement(3473..4039)
                     /locus_tag="ACGE4T_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8387402.1"
                     /translation="MTSFVNPFFSWFQIGFSYKIFLSLLNRKQKNNIMKEKEGVEMDK
                     RQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHIGKIRDPNVTKLANASYM
                     TRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRNRHEQLHQKWVQRDAT
                     VFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4142..4429)
                     /locus_tag="ACGE4T_002178"
     CDS             complement(4142..4429)
                     /locus_tag="ACGE4T_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8387403.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4600..5952)
                     /locus_tag="ACGE4T_002179"
     CDS             complement(4600..5952)
                     /locus_tag="ACGE4T_002179"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFF8387404.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6225..6671)
                     /locus_tag="ACGE4T_002180"
     CDS             complement(6225..6671)
                     /locus_tag="ACGE4T_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8387405.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1822 a         1328 c         1099 g         2481 t
ORIGIN      
        1 ggcttcattt tgagttatct gatagtatgc ttaattatca taatttgtct gcaccatctt
       61 tttttatcga aaacaatacc tcgttattcc aagaataaaa aagctgaaaa attttgttta
      121 ttattaaata aatttacttt aattgctcct atccttgcat ttattatttt ttctgtactt
      181 ttaagcacta ctcttaaagg aaaatttatg gaaagaagct ctcatgcaat gattctcact
      241 tttttatggt tgttgttcac cagaatctat atttttctga tgagcctgaa accgcccaaa
      301 tccattagtt tatgtttagt tataaatggt atttttttat tatcacttat catttttatt
      361 acacctttag atagatacgt cacttattta tataatcctt tggaatactg gacttatttt
      421 ataggtatat tggaaggcat tattttctac atcgggtatt ttcccaataa aaataacaac
      481 ttttattttt atcgcaataa attgtaataa gttaccatcc tagctactga gtatgtacat
      541 ttactttttc atgcaaaaaa gataaacgaa acagcttttc tccataataa caagataatt
      601 catacattta tttgcaatta aagaaaccac gtctacaggc gtggtttctt ttttagcgcc
      661 ttttctttta gacgcatccg atttttccca tcttgacata ttgatagcaa aggacatacc
      721 tcgcacttgg gatttcttgc cgtgcaatga tatctaccaa aaaaaatcag tgtatgatgt
      781 gtctttaccc ataattcttg tggtactttc cgcatcagtg tttcttctac ttccatgacc
      841 gtagcatcta gtttgcagat cctcaggcgt tttgagacac gttccacgtg ggtatctact
      901 gcgattgctg gtatcccgaa tgcatccccc aatacgacat ttgctgtctt tctgcctaca
      961 ccaggaagag acatcaattc ttctcttgac gttggcactt gcccatcgaa acgttcaatc
     1021 agctgctgtg cacacgcttt gatgtttttt gctttatttc gatacaaacc aatcgttttt
     1081 attttaagaa tgatttcatc gattgaagct tctgctaacg catcgggtgt cggaaaagag
     1141 gcaaacaaat ctggcgttgc tttattcact gatacatctg tcgcctgggc acttaagatc
     1201 actgcaatca ataactcaaa tggattattg tgcttgagtt ctccatgtgc ttcgggaaac
     1261 attccataca tcgtttctag agcttccatt gttttttgtt tgcttaacat attccgctac
     1321 tcctccaacc aatttttcag tgagactttc ggcaattctt cttgttgagc tgactgttct
     1381 acttctcttt gaagaagctg ctgttttctt cgtttttggt cttcttccac ttgttgtttt
     1441 gtacgtagat tttttctttc ccacgatagc aaaatgcggt caacgtaatt aaaactataa
     1501 gcctgattta atacggcttc ccgcagtgct agcttcaaaa tttccggctg ataatggtct
     1561 tcttctagcc attgtccgat ccgctggaac tcgatcgaag aaagcgggcg tccaaattct
     1621 tgttcaaata attgatacac tgactggatt tctttttctt gtgttttttc ttcgtatttt
     1681 ttttcttgtg tttttaaata ctcacctaat agatcataga tggggtaaag attatatcga
     1741 tctgcttttt taccgttgac aatcgtcgtt tctatcttga taaatccatt tgttacgaga
     1801 cgatccagta tttgaaaaat ctgatcttgt ttgattccca tatccatagc aatcatctgc
     1861 aaatcaggga aagtatctcc ttcaagctgt gccatatgca actgtagaac aaataaaaat
     1921 tcttcagatt gtagaccgat cctatgataa tttttaagta aaagattaga tatgactgtt
     1981 tgtccagcgc ttaagtaatt ttctaaattc aacatgattt ctcctccgct caatcacagt
     2041 atacgtaaaa aaaacatggg aagcaagaac aggtttcttt tctatttact tcactgatcg
     2101 gtcagattta caatgtcgtc tacttgattt ttgtttgttc atgtttttga tgcgtatctt
     2161 cacttttcgg aataaataaa ttaagtagat ttcgctgttc cttagaatgt tgacaaggaa
     2221 acaaaattac tatacactat ttttgtttcc aagttaacat tattgcataa aggagttttt
     2281 acatgtacag tttcacaaat aaacattcta aagtttttgg atacgtctct gtcttattca
     2341 ctggattagg atttactatc gtcagtcctg tacttccgtt tttaacgctg ccttattctc
     2401 actccataca tcagcaagct ttctatatca ctttgctcat gtctgtttat gctctagctg
     2461 ccttcttatc tgcccccatt ctcggtagct taagtgatca ttttgggcgt catccgatcc
     2521 ttattatcag tttattagga tcaagtatcg gatatttgct ttttggttta ggaaattcga
     2581 tatggatgtt gttccttggt cgtatcattg aaggattgac ggcaggagaa atctccacct
     2641 tatatgctta ctttgccgac atcacggaac ctaatgaaag aaccaaagtt tttggatgga
     2701 tgggtgcttt agtcggtata ggaacaacgc tgggtcctat tatagggggt ctattagctg
     2761 agttggggaa ttctgttcct atatttattg gagcactctt tactttctta aatgccgttt
     2821 atggctacac atttatgcta gaaagccttc caataaaaaa acgttcagta gatttaagtt
     2881 tctcccatgt aagacctttt caccaattac aacaattatt caagatttca tccgttattc
     2941 ctttattgac agcaggtttt gctgtttggt tggcagctgg ctctcttcaa tctatctttt
     3001 ctcaattttc gattgacgcc tttcagtgga aggctgggtt ggttggttta agcttctctt
     3061 tgattggaat cttggatact gtctcgcaat tgttcattat gcctcgattg ctcaaaaaat
     3121 tttcagaaca gcagatcaca agaataggga tgttcagtga aatcctcgcc tatctattca
     3181 tcacattatc tggaattctt ctactgccga ttttgttttt atttgggatt atctgttatg
     3241 gtttcggtga ttctattttt actcctgtat tcaatggcca actatccaat tctgtctcag
     3301 ataaccagca aggtcttgta atgggcggta cccaaagtat acaatctcta tcgcgcgtca
     3361 tcggcccgct aatcgcagga cagctttatg ctgcagcacc atttcttccg acggtattcg
     3421 gatttttgct tatgattttt gcctattatc actacaataa aaaatagtag cttcatttgc
     3481 tacttcgtat aaattctctg tatcgaccga taaaacgaaa tactgtgtca aattcttctt
     3541 ttgacatatt ttcaaataca gtagcatctc gctgtaccca tttttgatgg agttgctcgt
     3601 gtctattcct tattttttgt ccgattggcg tcaattgaaa atagatttct tttttattct
     3661 cttcgctttg ataagtgttt atgatccctc tagctatcag tttttttgtt agcttactga
     3721 tggctcctct agtcatgtaa ctagcattcg ctaattttgt cacgttagga tcacggattt
     3781 tcccgatatg ttcaatcact tctagttcat ttagagtaaa tcctgcaaat tctttttcca
     3841 tttctggttt attacgatga ataatctcat tagctaaatc cagatattct tctattagca
     3901 gttgtctttt atccatttct acaccttctt tttctttcat tatattgttt ttttgtttcc
     3961 tattcaacaa agataaaaat attttgtagg aaaacccaat ctgaaaccaa gaaaaaaagg
     4021 ggttgacaaa gcttgtcata accccttttt ctgattaaaa gatagtaaag agacagtcat
     4081 cggtaattgt gatgattttt tgtcatcact taaggatgag cggccttttc aaaaactgtc
     4141 tttaaagatc atcatcagtt tctagcacat atggctgacc ttcatatgtg tactctgtat
     4201 tcaatgtgcc gtcattttca tctgagtaga tgcgtctact gaaaggaaaa cgatgagaaa
     4261 cattatcttt ggcgtcattt gctggcatcg tgtaatattc ctcttgcgtt tcattcaact
     4321 gagagagtac ttgatctgcc aactcatcaa tattgatcgt taatttcttc gaagcacttt
     4381 tataatcttc taaaaatgca tcttgttgga caaaatattc tttattcatc ttctttctca
     4441 cctttctttt caaaatcaaa taaagctgct tgttctttta tgtaacagct ttatctttgc
     4501 tgcttattgt gacaaaaaat tcgaagaaag acaactaatt gtacttttca caaaaaaaga
     4561 cagagggtcg tgacaaaatt gtgtcacaac cctttttctt tattgtgcag tttcgatttc
     4621 gcctagcgct gcttgtgcaa gtaggttcaa gtaattccat gggcggtcaa aatgtggttg
     4681 gaagaagaaa tctgaaagtg cgagatcttc tacagtcatt ttattttgaa cagctaaaga
     4741 catggtattt gctgattgtg taatgtcgta tttggacatg aactgagcac ctacgatacg
     4801 attcgttcct ttttcataga ctagctccat cattactttt tcagtagttg gcatgaactc
     4861 tggacgataa ttatcttcaa atacagtcgc ttggacttct agatcgttca aagttgcgct
     4921 ttctttggtc acacctgtcg aaccgatcgt ccagccaaat aggtaaagtc ctgaagttcc
     4981 ttgagtacca cgatacgcca ttttatgatc tgtcaagtta tttcccacaa gcaaaccttg
     5041 acgaacggca tttgttgcca aaggaatata gttttgcgtg ccacttggat tgtagttgac
     5101 tactgcagag tctccagctg caaaaatgtc tggattactt gtctgcatgt attcattcac
     5161 tttgattgcc ccgtttggca gtgtatctac ttttccttcg atcagttttg tatttggacg
     5221 gaagcccaca cataaaatga ccatatctgt atcaaatgta tctgttggtg tagctacttg
     5281 tttcactttt ccttcttcat ctgtgataaa ttcagtaaca ttttcgccta gcgcaagagt
     5341 aacaccgcgg tctgtcaatt ctttttctaa gatatctgtg aatggtttat ctagatattt
     5401 attcaagatg cgatctaatc catcaatcaa tgtcacttgt tttccagatt cgacaaaagc
     5461 ttctactaat tcaataccga tatatccacc accaacaatt actacacgtt ttgcttcttt
     5521 cgcttgtgca ataatttcat ttgcttgatt gtagtttttg cataggagta catttttcgc
     5581 atcgattcca ctaatcggtg ggataatagg ccatgaacca gttgtcatca ctaatttatc
     5641 ataagaaacc gtttctgttt ctcctgtttt cagatctttt gccgttactg ttttattttc
     5701 tgtatcgacg tctgttacat cgtgttccat tttaactgtt gctcctaatg aagataattc
     5761 ttcaggattt gagtagaaca agcctgctgg gtcctttact actccgccga cataaagtgc
     5821 gataccgcaa gataagaaag aaatattgtc atttctttca tatactgtca cttctgcttc
     5881 tggatgattt tttaaaatac tttttacagc tgcagtacct gcatgagtac agccaattac
     5941 tactactttc atttatggtc cctccgatta acattttgtc cttcgacaaa actactataa
     6001 cagaaataaa acggatacaa cagtttaatg cttgtgaatt cacaaaggtt tgtttgtagt
     6061 ttcattcctt ttcaagcata agaacccaat atctacaaga ttcttatttt aataatattc
     6121 acttttatat ctaagattgt gaataaaaga caaaaaaata aaaaagctag cgtttttttt
     6181 acacagctag ctttttttag gtataacact tgtagaaact gtttttacat ttgcgccttt
     6241 tttgctgact ctttacaatc cggacagagt ccatacaatt ccaaccgatg ttcattaatc
     6301 tcaaaatcag tcagtctgct tgcagccatt tctatatctt caagccctgg gtaatgaaaa
     6361 tcaacaatct taccgcagtt ttcacaaatt acatgataat gacgctttga actgaaatca
     6421 aatcggctag aagcatcgcc gtacttcatt tcttgaacaa atccaatcga agtaaataag
     6481 cgaaggttat tgtaaacagt cgcaacactc atatttggaa accgatgttc aagtgcttta
     6541 taaatctcat cagccgttgg atgggaatga tgttcaatca aatattctaa aaccgcgtac
     6601 cgttgaggtg tgattcgaat gttctctttt tttaactttt cgataacctc ttcgactaac
     6661 ttattttcca tgtaaacacc ccacgttgtt attctcgtct tatcatacta tttttacatt
     6721 ttaaaatcca
//
LOCUS       DAXWLG010000134         6568 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXWLG010000134 DAXWLG010000000
VERSION     DAXWLG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.136"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            102..311
                     /locus_tag="ACGE4T_002181"
     CDS             102..311
                     /locus_tag="ACGE4T_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFF8387406.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(709..1221)
                     /locus_tag="ACGE4T_002182"
                     /pseudo
     CDS             complement(709..1221)
                     /locus_tag="ACGE4T_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(1347..2048)
                     /locus_tag="ACGE4T_002183"
     CDS             complement(1347..2048)
                     /locus_tag="ACGE4T_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8387407.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(2156..3599)
                     /locus_tag="ACGE4T_002184"
                     /pseudo
     CDS             complement(2156..3599)
                     /locus_tag="ACGE4T_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(3613..5478)
                     /locus_tag="ACGE4T_002185"
     CDS             complement(3613..5478)
                     /locus_tag="ACGE4T_002185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8387408.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(5599..6450)
                     /gene="licT"
                     /locus_tag="ACGE4T_002186"
     CDS             complement(5599..6450)
                     /gene="licT"
                     /locus_tag="ACGE4T_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8387409.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         1878 a         1472 c         1099 g         2119 t
ORIGIN      
        1 agccatttta tagataaaac aaaaataaat aacgtgtttt catcgatatg aaatgactta
       61 cttatagaag atactgggat ttcgtcctat atgccaataa cgtgtattat tttaaacaac
      121 aaatacttgt ttcttttatt aacgaaaaaa tcattttgtt ctcgtgttat gtttcactga
      181 taaatgatgt ttctctaaat aaaaaagccg acgaactaaa cgatcaaaat ttgatcattt
      241 ttgttcatca gcttctccta aaaatctttt tgttaacgat aaatagctgg atttgtaaaa
      301 atatcacttg aatcgtcact gctggaagaa aattcagtga taaccgcacc tgttgcgcct
      361 gcctggaact agtgtttgat attgggctcg aacgtgtact gatctctagc atgaagaata
      421 atttcttttt ttactgtata ataactttct ttcctttttg gaatagtaca ggaaagtttt
      481 tttgtttcct ttccttcgac ataaggtata ccagaccctt tcgacaacga aaggttctca
      541 tttttccttc atcgtcattt caaggagggt gccgatgctc tggacagttt cgatcgctcc
      601 taaaccaaaa tctgtatatt cgattctttc taactctgat tctttcatac cattcctgtc
      661 tcaaggtaca tctattgtac ttttttcttg taatccattc tgcctctcct aactaattgt
      721 caaatcatcc caagatatgt cttcttcctc tttttcttct tggatgattt ctgcattcgg
      781 atctactggt tttttcaata ataaaatggt tattcctgtg acaaaagaac cagctaacaa
      841 tgcaccaatg aatgcccatg gtctggtcat cgtagcgact gcaaacattc ccgatgccgg
      901 aatacggcta tctgcgcccc aaaacatgct taatccgcca cctactgtcc caccgactcc
      961 cgttgctatg acattgcgtg ccaagtcgtt caatacgata gggaaacatc cctctgtgat
     1021 ctgacagatc cccataggaa aagcggtctt caatgtatcg atttcttgac gattgagtat
     1081 atttttacga atgattttcc ctaagaaata agcgatcgtc aagccgaatg gggttaccat
     1141 cgaagctaaa atcaatacag tgattggttc atagattcct tctgaaaatc gtgccacacc
     1201 cccttatcca gaataaatgg taatccagaa acagccgtgt atctgcggta ataagcctaa
     1261 ccaaacgaaa aatatgagaa gctatttttg tttggctgtt cttattactt gatgcagggc
     1321 agctttttca caacctcttt ttttatttat gtttcataat caatatggta aggatgcagc
     1381 tccgtatggt agaccgcctc cgaatattca atcatttccc caatttgcgt agacgatttc
     1441 cgtatccgtt tgatgccgat ctgtgcgtct gttttcagaa ttccttgttc ttcttctgtt
     1501 aaaaatactg ccgagaaatc atcagaaaat gagtcaatag tatatccttt tttcgtcagc
     1561 aaacgataca aagattcttt gccaaattct tctctgctga attgcatcat ttcttgtgga
     1621 agataatgaa caaaataaat ataaggctga ttgttcaaat aatataagcg cttaaaacaa
     1681 acagcttgct caccgaaaaa ggaaaacaaa ggatcttcct tctttatatg gaccaattgc
     1741 aaatccagcg tttctttccg gacttcatat cgggagttct ccaagatttg cgtaaagctt
     1801 gccgctttgg ataatcgatt gtaaggacga ttgcttagaa ccgtcgtccc acgaccactt
     1861 ttctttgcga catattcatc tgatgccagc agttcgattg cctttcggac agtgattttg
     1921 ctgacttgaa acagttcttc taattcactt tctgtcggca acatcgaacc gactggatat
     1981 tttcctgaga aaatatcttc tttaatttta tctgcgactt ccaagtatag tacacgtgtc
     2041 cttttcataa cgtcaccctt tgctttacta ttactaaaag aatagcacat aaaaaataaa
     2101 aaaagaacac aaatcacatg tgagaagtgt gttctttttc attcagctgc tttaattaca
     2161 agtttacgcc atttgaggca attacttctt tataccaatc aaaagatttt ttctttgatc
     2221 gattcagtgt cccatttcct tcatcgtcta gatccacata gataaagccg taacgtttac
     2281 tcatttctcc tgttgatgca ctgacaagat cgatacatcc ccaaggcgta tagcccatca
     2341 aatctacacc gtctgctact gcattagcca tcgcttcgat atgacgacgt aaataatcga
     2401 tccgataatc atcaataatc gtgccatcct cttccatatg atcaatggct ccaagaccgt
     2461 tttcaacaat gaataatggt ttctggtaac gattgtataa ttcattcaat gcgattctca
     2521 gcccttctgg atcgatctgc cagccccatt cacttgcctc aagaaacgga ttttttactc
     2581 ctcccagcaa gttcccttct gaagttgctg cttgtggatc agtctcattg attgccgtag
     2641 acatataata actaaatccg atgtagtcca ctggatattc ttttagcaac tggaagtctt
     2701 tttccgtttg ctctaatttt tccggatcga ctccatattt tttatgggta cgcgctgtgt
     2761 atgcagggta ttctccacgt acttggacat ccgtgcagaa aaaattgaat tcttggtttt
     2821 ggatcattgt tgctgcctga ttgactggat tggcatcgat gctgtaagtt gttgcataga
     2881 taatcatgca gccgatttgg atatccgaac gcaattcgtg gccgattttt acggctttgc
     2941 tacttgcaac gaattgatta tgccatgctt ggaaaatatt ttggtactct cctgctccat
     3001 tgctttttac taatccttga cttaaagctg gaaagtgaaa ggccgaattg atttcattga
     3061 aagtcatcca atatttgact ttactgctgt atctctctaa tactttctga gcaaatcgtt
     3121 catagaagtc gatcagtttt cgatttttcc agccaccgta ttcttttgcc aaatgaagag
     3181 gcatttcgta atgagaaatc gtgatcacag gttcaatatc atattttaag cactcatcaa
     3241 tcagctgatc ataaaattct aatcccgctt cgttaggagt accttcgtct ccatttggaa
     3301 aaattcgtgt ccaggcaatc gaaaaacgat aacatttgaa tcccatttcc gcaaacaagg
     3361 caagatcttc tttgaaacga tcgtaatgat cgattcctcg atgattcggg taaatatatt
     3421 tttcttgatc aatcgtccag tcaaattctt cactgccgat aatcgcaaag cgctgcttac
     3481 ctccaggcat agcatctgca acggatagtc cttttccgtc tcgatcataa gccccttcta
     3541 attgatttgg cagcagttgc tccaccccat aaaaattttt ctgaaaaacg tgattgcatc
     3601 ctgatttcct ccttatttta caattgccag aacatcttcg tttgcaataa tctctttttg
     3661 gtctaattca agtatatcgg caaatgcatc agtattcgta atgacaactg gagtgattac
     3721 gctatagcca gctgctctaa ttgcttcgat gtcaaaacga atcaataaat ctcctgcttt
     3781 gacgagatcc ccttgtttga ctaagatctc aaagccattc ccatttaatt ctactgtatc
     3841 catcccgata tgaatcaaaa tctccgctcc gtcagtcgtt gtgatcccta cagcatgtcc
     3901 agttggaaaa atagtcgtga tctctccgtc tgcaggtgca actagttctc ctacttctgg
     3961 atcaatagcg ataccttttc ccatcacgcc agaagaaaaa acttggtctg gtacttcact
     4021 caatggcagt actttacccg ttaaaggact ttttaaggta tgccgtgctg aagtaatcgg
     4081 ttctcctgca ttcgcatgtt tgttttctaa ttgattgtct tgtgtttgtt cgtcaaatcc
     4141 taaaatcaat gtacctacga atgcaagaac aaaagcaatt cctgtagcta ttactgctac
     4201 tgtcacattt gactctacac catccaccgt attaatgaaa gtcggaatcg tcaataagct
     4261 gaccaaactg ctggagaaag cttttactcc ggaaaatccg ataatagctc caccaatacc
     4321 tccagaaata caagcggcaa tgaatggttt cttcaaaggt aatgtcacac catacacagt
     4381 tggttcagtg attccaaata acgaagtcac tgtagaagaa atccctaatg ctcgaagttt
     4441 cgtatctttt gttcgcaatg ctacagctaa agcagcccca ccttgtgcca agatggctgg
     4501 aagaagcatt ggcatcaata catcaaaccc atattgttcg atattcaaaa acatgatcgg
     4561 tacgaatccc caatgcatgc cgaacatcac gaatacttgc cataaacttc ccatgattag
     4621 acctgctacg attggactaa atccataaat cgagttgaag agaccgccta aagcattacc
     4681 ggcaaccgtc ccgattggtc ctaaagcaag aaatgttaat ggaaccatca ctactaaaac
     4741 gacaagcgga actaaaatca tttgcagaaa ttgcggaatc acttttttta cgaatggctc
     4801 taatttactt tgaacccata cggctagaat gattggtatg acagaagaag tataaccaga
     4861 tggtgaaaga atcactggga tcccaaagaa actgatagct tctcccgcac cagcaactgc
     4921 tgtgatagct ggatacacaa gtgccatagc aattgctact gctaaaaact ggttggcttt
     4981 gaacttttta gctgctgtaa aagcaagcat gacaggtaag aaggtaaaga caccatctgc
     5041 tgcagcaaac aatatttgat aagtaccgct atcagctgtc agccagccca tcaccgttgc
     5101 caatgataag aatcctttca ataccccggc ggctgccatc gcccctagaa aaggtgtaaa
     5161 tatactagag ataatatcga tcagacgatt caaaatattt ttggactctt cttttccatc
     5221 ctccgaaact gcaagacctt cctcatccac gatcgattga taaacatctg ctacattact
     5281 gccaatcact acttgatact gaccgccact ttgtacgact tggatcacgt ccgggtcttc
     5341 ttttaacttt tgcgtatctg ctaaagattc atcttttaat ctgaatcgca accgcgtagc
     5401 acaatgtact aaacttttga cattcttttg tcctcctacc gcttcccata cacgacgacc
     5461 aactgcttga ttatccattg ttttttcctt ctttcttttt tatttaaaaa ataaaaaccc
     5521 agattgaaaa aaacagtcac aaacattcgt gaacctattt tttcaatcca ggttttgcct
     5581 gccatacagt aacaatcctt atgattggat gctttttttc tcacttacta ctctttgaat
     5641 atgaatcgtt aaataaatct gctctgcgat ccccatttcc tgtttttgcg tttgtcgaag
     5701 atactcattg atccgttcga cacaatgaaa cgcttttgga tactttcctt gaactaatgc
     5761 gtagagaaat tcatcttgct cgtcagatcc ttcccctttg agataacgct gggcaaaaaa
     5821 ctgcagatga gtaatgatac gctgataatt caaagattct tcatcgaaca cgatgccgaa
     5881 gaatttactg atgatactca gtatatctct taccattgtg gtagcggtca tcgtcacttg
     5941 catgtcttcg tttccttgtt ggctattcac aaaatgaaag gcaataaaac cggcttcatc
     6001 ctcagaaagt tccacacccg tcttttgttt cacgaacaaa agagcctctt tcgctgcttg
     6061 atattctttc ggataaaatt tttttgtttc aaataataat ggattaggca gttggatatt
     6121 ttcttttgct cgtgtgatcg cataatgaag atgatcggtc aatgtcagat aaatattagg
     6181 cgaaaattca acttttagtt tatcttcagc cagttcgact acttgtttga cccattcgat
     6241 ctcctctgcc ggaatatcag ccatcagctg ctggaattgg tttttagcaa aagaatctct
     6301 taaaacaaat tctttttgga ccgcttctgg cgaaatctcg tccccaatct ttttttgaaa
     6361 agctagtcct tttcccatac aaacgacttc ttccccgaat ccatttttcg aaatgacgac
     6421 attgttattc aaaattttat caattttcat tttgcttcgc cctctcaaga aaaataaaaa
     6481 aacctagaat tcgcctactg tttcttagct gaattctggt tttgcctgcc gtacagtaac
     6541 aatccatttc ttattcaaaa tcatcata
//
LOCUS       DAXWLG010000135         6515 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXWLG010000135 DAXWLG010000000
VERSION     DAXWLG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.137"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            236..2002
                     /locus_tag="ACGE4T_002187"
     CDS             236..2002
                     /locus_tag="ACGE4T_002187"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFF8387410.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2104..3363
                     /locus_tag="ACGE4T_002188"
     CDS             2104..3363
                     /locus_tag="ACGE4T_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8387411.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3510..4811
                     /locus_tag="ACGE4T_002189"
     CDS             3510..4811
                     /locus_tag="ACGE4T_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8387412.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4814..5665
                     /locus_tag="ACGE4T_002190"
     CDS             4814..5665
                     /locus_tag="ACGE4T_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8387413.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5678..6493
                     /locus_tag="ACGE4T_002191"
     CDS             5678..6493
                     /locus_tag="ACGE4T_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFF8387414.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         2071 a         1140 c         1327 g         1977 t
ORIGIN      
        1 aacttttact ttagaaaatt ggagaaattg atcgcttaag ataaattcag gacactaaat
       61 agtacaggaa gtagattaaa acttttaaaa gttttaagtc tatctatttg gcaagtttaa
      121 aaaataaaaa ttcggtatca aaaaactgtt accgataaca tttttgaaag cgttgtcttt
      181 tgtttctcta tcacttatag tgatgacgtc atattaaaac acaaggagga aatcaatgga
      241 tacagctgca atttatcatc gaccggaaag tgaatatgca tttctatata cgaatacaag
      301 atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg tgctttgtgg
      361 agatccatat accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg
      421 tctaagtacc aatgttcatg attattggga aattgaagtg acgagcgaaa caaggcgctt
      481 acagtatgcg ttccatgttg ttggggaaga taacacggac tgtttttacg gtgatcaagg
      541 aatttttcct tatcgagaaa atgtgattac tgaaccaaat ttttattttc gaatacctta
      601 ttttcatcaa atcgatcgat tcactattcc agaatgggta aaagaaacag tatggtatca
      661 aatttttccc gaaagatttg caaacgggga caaagcaaat gaccccgaac acactttacc
      721 ttggggaagt aaagatccag accgagaaga cttcttcggg ggagatttgc aaggtgtgag
      781 ggatcatttg gattacttga cggaccttgg cgtcaatggc atctattttt gtcccatatt
      841 taaagccaca tccaatcata aatacgatac gattgattat tatgaggtgg atcccgcttt
      901 tggcgataaa aaacttttaa aaaacttgat tgatgaagca cataaaagag ggatacgtat
      961 catgttcgat gctgtattca atcatatggg tgtccattca ccacagtggc aagacgttct
     1021 caaaaacgga gaaaaatcga tctataaaga ctggttccac atccgttttt tcccagtaga
     1081 ttcttatcag atgactgact taccagaaac tgctgaaaat atcccatatg atacttttgc
     1141 ttttactcca ttcatgccga aactaaatac agcaaatcca gaagtgcaaa aatatttgct
     1201 ggatatcgca acttattgga taaaagaatt cgatattgat ggatggcgtc ttgatgttgc
     1261 taatgaagtc gatcatcatt tttggaaaaa attcagagaa gcagttacag aaatcaaacc
     1321 agacatctat atccttggag agatttggca ttcctctcaa gcttggctgc aaggggatga
     1381 attccatgca gtgatgaatt atgcattcac agattcgatt aaagattatt ttgcaaaaaa
     1441 gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc tttacagaga
     1501 tcaggtgaac gaaggaacat tcaacttatt agattctcat gatactgcca ggatcttgac
     1561 cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa
     1621 agggtctcct tgtatctatt acgggacaga aatagggatg actggtgaag atgacccgga
     1681 ttgccgcaaa tgtatgatat gggagaagga ggaacaagat ctagagctat tcggttttat
     1741 aaaagaactt gtttctctaa ggaaacagtt gtctaagatt atttctgaag gttctactca
     1801 gtggctaata gtatatgatc gtgaagataa gctctatttc acacgggaac tagagggaca
     1861 gatcatctat gtttacttta atcagtcaaa agaaccttgg gtagtagaac aggaaaatga
     1921 ggtgattctt agtcaaaatt gtcagttgtt agaagacggg aaagcagaga tacgacaata
     1981 cggatttttg atcatgtgtt gaaaactgtt accggtaaca acaagaaagc gtttgcaaat
     2041 gaagatttgt gcccattata ataagggtgt aacagcacgt tgaatacaca ggaggaaatg
     2101 aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt taatctcttt aggaatctta
     2161 ggcggattag cggcttgtgg caatggtaac tcttcagcag aaggggaagc cgatgaagga
     2221 aaaacgttaa cagtttccgt tgattctgga tataaagatt atataaacga aataaaaaac
     2281 actttcgaaa aagaaaacga tgtgaaaatc aagctagtag aaaaggacat gttcgatcag
     2341 ttggaatcat tagcgttaga tgggcctgct ggaaagggac ctgacgttat gatggcagca
     2401 tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa actgggaaat
     2461 gaagcagctt acgatgaaac agacaaagcc caagtgactt atgatggaaa aatctacgga
     2521 gaacctgcag taatcgagac ccttgttttg tactataaca aagatttagt cgatacagca
     2581 ccatcaacgt ttaaagattt ggagaatcta tccaaagaca gtcgttttgc ttttgagtca
     2641 gaagcaggaa aaaatacagg atttttagct aaatggactg acttttatta ttcctatggt
     2701 ttaattgcag gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc
     2761 ggtttgaata atgcaggtgc tgtagaagga atctcttatg caacagattg gttcaaaaat
     2821 gtttggccaa agggaatgca agacataaaa agtgcaggag acttcgccag ccagcaattc
     2881 atgtccaata aaactgcagc aatcattgac ggtccatggc aagcgcaaac atacaaagaa
     2941 aacaatatca attatggtgt agccaaaatt cctactctaa ataatggaca gccttatcaa
     3001 ccgtttggcg gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa agacctctct
     3061 caaaaatggc ttgattatgt aacgaatcag gaaaatcaag aaaaattctt tgaaatggta
     3121 aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa cgatgaatta
     3181 acaacagctg tgattgaaca atatgaaaca gctcaagcga tgccaaacat cccagaaatg
     3241 ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca
     3301 ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg aagccataga acaaaaatac
     3361 tgattaaaat gataaccgtc catttttata aaaaatggaa caggtgcttc agataaaagt
     3421 ggcaagagcg agatggaaaa ttatcttgct cttgttgcca ttatcttaaa agcagtttgc
     3481 ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga taaaacaagc aatatcaacg
     3541 aaaaaaaaat cacgctttgt tcacttattc cagggatggg gcagtttgta aatggccaag
     3601 cttttaaagg tatcgttttt ttcgccatat tcgtcctatt cattggtcag atgcttctgg
     3661 gcggatttca tgcattggta ggattggtta ctttgggaag tataccaatg gaagatcatt
     3721 cattatttat tttgattgaa ggcactttgc aattcatcat tacaatcatt ttcttgattt
     3781 tttacggttt gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa
     3841 aaatcaatcg gacgatgaag gaagtcatac ataatatagg tgatgaaggt tttccttatc
     3901 tattgacact tcccgcttat cttctcatga tttttacgat cattttccca gtcctagtta
     3961 cattgttcac tgcatttact aattatgact tcaatcatat tccaccagcc agtttaattg
     4021 attgggtagg ggctaaaaac ttttttagta tcttcttttt aagctcatat cgtaatactt
     4081 tttttgcagt cttttcgtgg accgtgattt ggactttatg tgcaacaact ttgcaaatca
     4141 cccttggtat tctcacagcg gttgttgctc accagaaatt tattaaattc aaacgtattt
     4201 tcggtatcat tttcttatta ccatgggcgg tcccagcttt tattactatc atgagttttt
     4261 cgaatatatt caacgatagc gtaggtgcca tcaatactca agtgattcct ttcataaacc
     4321 agcttccttt tgtagatatc agtgcaatct cttggaaaac ggacccgaac tggacgaaag
     4381 tagcaattat cttagtgcag ggctggttag gatttcccta tatctatgtg atggttacag
     4441 gaatcttaca atcgatttca gaagacttat atgaagcagc aaaaatcgac ggagcaaatg
     4501 catggcagcg cttttcaaac atcacgcttc ctgctgtctt cctagtggca gcgccaacat
     4561 ttattacaca gtatacgggg aatttcaata atttctccat gatctatctg tttaacaatg
     4621 gtggtcccgg aagcgtaggc ggaggagctg gttctactga tattcttatt tcctggatct
     4681 ataaacttac gactggaact tctccgcagt attctatggc ttcagcaatc acgttgatta
     4741 tctccgtcat cgttatctgt gtgtcattgc tggtatttaa aaagacaaat gcgttcaata
     4801 tgggggatta acaatgaaaa aatttcattc gagtgctcga ttccaaaaac gactttctca
     4861 cttttttact tacttttttc ttttaatact gtcagtcatt atcatctatc cgcttttgat
     4921 cacagcaagt actgccttca agtcaggaaa tattgcagct tttagtttag atctttcaag
     4981 tgactggact ttaggaaact ttactcggct attcagtgaa acactttacg ggacttggta
     5041 taaaaataca ttgatcattt ctgtcctaac aatgatcgta caagtgactg tagtaacttt
     5101 ggcgggctat acttatagtc gctatcgttt tatcggccgc aaatcaagtt tgatgttctt
     5161 tttagtgatt cagatggtac cgactatggc agcattgact gccttttatg tcatggcttt
     5221 cttattagaa gcgctcgata agtactggtt cttgacaatg atctatatag gcggtggaat
     5281 tccaatgaat acttggctga tgaaaggata ctttgacact gtaccaatag atttagatga
     5341 atctgctaaa cttgatgggg cagggcattt caggattttt ttccagattg tattaccgct
     5401 tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt atgggacctt ttggggattt
     5461 catgttagct aagttcctgc ttcgaacacc tgaaaatcag actgttgctg tcggcttaca
     5521 aacatttatt tctgatgtga caaaccaaag agtctctctt tttgctgctg gggcaatttt
     5581 gattgcacta ccgatttgtc tattattctt ctttttacaa aagaactttg ttaccggcct
     5641 gacatctggc ggaacaaaag gataggagca gagtatcatg caaacatttc cgttaaacta
     5701 tttttccgca ttacgtacgc caactcaagt gtttgcggga cgcaaactat tatcatggcc
     5761 taaatttttc ttgatatttg tatttttagt ttccttgatg gtcatgcctg tgactctttt
     5821 ttatgccaat cagatccaag cgattccatt agaacaattt ctatccgttc attcgctgat
     5881 cgatgaacaa ggaacccaaa aattttctga acttgaatta tctgaaactg ggttacaagc
     5941 atcgcaacaa acgatagctg tgacccctga aattttggta ggtgtgtctt tatctgagaa
     6001 acaacaatcc gatcatggaa cttttattga ttttgagaag gaacaatggg tgatccaaca
     6061 aaaggataaa tctggtatac gccgctatac gatgaattat agcccatctt tccaaccaga
     6121 ttctgtacgt acgcccgaag atttccaacg atttttagaa agagaatttt atgccagtaa
     6181 tcgtccaaca atcattttat cttatagttt aagtcttggt cttgtcctat ttgtcatgac
     6241 gagtctcatt ttattcgggg cttccttttt cttgtggatg acaagaaaaa gccaactttc
     6301 atccattcat acattcaaag agagtgcaaa tttgatgctg aatgttatgg gaataggttc
     6361 catggcagca gcagttgtag ggtttatcca ctttgacttt atcttgatgc taagtgtcca
     6421 aacaacgata actgtcttgc tgcttttatg ggtttttgtc aaaacaagat tcaaagataa
     6481 gcgtgtcgaa tgacaagttt aagcgattta tgcga
//
LOCUS       DAXWLG010000136         6403 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXWLG010000136 DAXWLG010000000
VERSION     DAXWLG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.138"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE4T_002192"
     CDS             complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE4T_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFF8387415.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE4T_002193"
     CDS             complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE4T_002193"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFF8387416.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            3463..4230
                     /locus_tag="ACGE4T_002194"
     CDS             3463..4230
                     /locus_tag="ACGE4T_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387417.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            4220..6262
                     /locus_tag="ACGE4T_002195"
     CDS             4220..6262
                     /locus_tag="ACGE4T_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8387418.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         1904 a         1131 c         1227 g         2141 t
ORIGIN      
        1 ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc
       61 ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga
      121 tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg
      181 tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt
      241 atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt
      301 tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa
      361 aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat
      421 gatttgttct tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt
      481 gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc
      541 ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt
      601 tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca
      661 aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta
      721 aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc
      781 catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt
      841 tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt
      901 tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt
      961 ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt
     1021 cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat
     1081 tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag
     1141 caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat
     1201 acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat
     1261 ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg
     1321 tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg
     1381 atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga
     1441 gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt
     1501 aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca
     1561 ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga
     1621 cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca
     1681 tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag
     1741 agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga
     1801 tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt
     1861 cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag
     1921 cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca
     1981 ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct
     2041 tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc
     2101 cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt
     2161 tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa
     2221 gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg
     2281 gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct
     2341 tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg
     2401 gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct
     2461 gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg
     2521 ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa
     2581 tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac
     2641 attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa
     2701 acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt
     2761 tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa
     2821 ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg
     2881 ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc
     2941 aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc
     3001 ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca
     3061 tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct
     3121 tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca
     3181 aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt
     3241 ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct
     3301 ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt
     3361 attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta
     3421 tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa
     3481 gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat
     3541 atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga
     3601 aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg
     3661 atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa
     3721 aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat
     3781 atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga
     3841 catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct
     3901 gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt
     3961 tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac
     4021 ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat
     4081 acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt
     4141 gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt
     4201 ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc
     4261 acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt
     4321 cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga
     4381 tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag
     4441 tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg
     4501 tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt
     4561 cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt
     4621 ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc catcagtagt
     4681 agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa
     4741 aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac
     4801 aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag
     4861 cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta
     4921 tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac
     4981 ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta aatggaagat
     5041 ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag
     5101 ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg
     5161 cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta
     5221 tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa
     5281 gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct
     5341 cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag
     5401 aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct
     5461 tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc
     5521 agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat
     5581 ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt
     5641 ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg
     5701 actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt
     5761 aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga
     5821 agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg
     5881 tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt
     5941 gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa
     6001 agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat
     6061 cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt
     6121 atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct
     6181 tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata
     6241 ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata gaactaaatt
     6301 ttgaatacca tttatttgga ataagaggtt gtgacacgat ttttgtcaca acctctttat
     6361 tttttatatt ttttaaaagt acgagaaatt ataaagaaaa aag
//
LOCUS       DAXWLG010000137         6260 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXWLG010000137 DAXWLG010000000
VERSION     DAXWLG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.139"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            352..1041
                     /locus_tag="ACGE4T_002196"
     CDS             352..1041
                     /locus_tag="ACGE4T_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8387419.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACGE4T_002197"
     CDS             1028..2236
                     /locus_tag="ACGE4T_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8387420.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACGE4T_002198"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACGE4T_002198"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFF8387421.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACGE4T_002199"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACGE4T_002199"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFF8387422.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1923 a         1176 c         1216 g         1945 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggta aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat
//
LOCUS       DAXWLG010000138         6206 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXWLG010000138 DAXWLG010000000
VERSION     DAXWLG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.140"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            305..554
                     /locus_tag="ACGE4T_002200"
                     /pseudo
     CDS             305..554
                     /locus_tag="ACGE4T_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(798..1628)
                     /locus_tag="ACGE4T_002201"
     CDS             complement(798..1628)
                     /locus_tag="ACGE4T_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFF8387423.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            1836..3170
                     /locus_tag="ACGE4T_002202"
     CDS             1836..3170
                     /locus_tag="ACGE4T_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8387424.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGISDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3211..4122
                     /locus_tag="ACGE4T_002203"
     CDS             3211..4122
                     /locus_tag="ACGE4T_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8387425.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLTPKWVYYSGGDGK"
     gene            4119..4973
                     /locus_tag="ACGE4T_002204"
     CDS             4119..4973
                     /locus_tag="ACGE4T_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8387426.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            4970..5587
                     /locus_tag="ACGE4T_002205"
     CDS             4970..5587
                     /locus_tag="ACGE4T_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8387427.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            5589..6107
                     /locus_tag="ACGE4T_002206"
     CDS             5589..6107
                     /locus_tag="ACGE4T_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8387428.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1849 a         1105 c         1241 g         2011 t
ORIGIN      
        1 gggatttctg ttgaatatct aataaataaa gatttcatag gttcttgttt ttatacggct
       61 ttatttcttg gacttcttga agcattttcc atttataaaa aatcaaaaaa ataaaataga
      121 ttgccgtaag agatcttttt catttcattt ttttctgaaa acccgttgtt tctgtcagtg
      181 aagcagcggg tttttagatt tattgtcttt agacctaatt gagcgcgccg agcgattgaa
      241 atacaaaacc acttgctatg cgggtggagc aataaaggga aatagtttag cccgtacaaa
      301 gtagatgtgt aaataccaca ttgtattcac accaaagtat agaagaaaaa taatttataa
      361 tcaatatcga gccaatattc aggagatcat caagagattg tgcaaatcta aaatataaat
      421 ttgggtatcg acacttttgg gtggaaggat actatgtgac aaaagtagga caaaatgaag
      481 cgaccatcaa aaagtacatt caagaacaag aaaaacatga tagtgcgtta gataagctga
      541 gtgtacgaga gtaagacgat ccttttaagg gctaaggagt agtacataaa ccttttaaga
      601 ggtgggcaaa actggcaaag gcaatatggc ttgaacataa aatatgaaag ccagcgtctt
      661 taggcgctgg ccggtgattt gagcttatag ctctagagaa aaccaccctt ttgaagggtg
      721 gttatgatta atgactttag tcagttttgc tcacaaagcg tgtggaacaa aaaacgggtg
      781 tttctacggg tagatattta tttcttgaat ggaggtgtcc catacatttt tggtgttact
      841 ggttctaatt ttggtgcagc gccttcgatc ttaggttctt tcaaccattg atattcgcct
      901 tcgccatcca gtgctttgcc actagcccaa gaaccttcac tactttggtc tccctctgag
      961 aagttataga agtcataagc aatgtctttt cgttctttgt cacgcgggaa agtacttggg
     1021 actaagattg aaccttcttt ttcttctaat tctttcactg cacattccca tagattttga
     1081 tgatagctat ctcttgcaag gagaacagac agcatatctt ttacgccacg atcatcgatc
     1141 atttcgtaaa tacgtgctac ttgaagtctt ccttgtgatt cggcatttaa attagctcta
     1201 aagtctgcta acaagttgcc actcgcaatg atataggcac cgctccatgg attgcccacg
     1261 ctgtcagcag gacgaggtcc taagccagct acgatcgcgt gctgtggatt cattccagag
     1321 ataatggcac cgattgttgg atctttttgc atcgctgctt cttgatcttc aacaggcgca
     1381 ccctctaata aacgagcaac cattgtcgcc aacatttcca cgtggccgat ttcttctgtt
     1441 gcaatgtcca tcagcatatc tttatatttt tcttctcctc ggcagctcca gccttggaac
     1501 aggtactgca tagctacact catctcgcca tattggccgc cgatcagctc ctgcagatat
     1561 ttcgcgatta atggattcgg acgatctggt tttgcttcaa actgcaattc tttctggtgt
     1621 ctaaacatca aaattcctcc taatctattt ttaatacatt ctcattctgc tacgaaagat
     1681 taaaaaattc aaagaaagcg ctcgcgaatt atataaagta ttggtatcat taataatttt
     1741 tttagaatca taccttataa attgttattt tcagaacaaa atgatatcgt tatattaaaa
     1801 ccggcatcgg ttacattaaa atttggaggt aaaatatgaa aaaattattg tttacagggt
     1861 tgctggcaag ttcagcattg tttttattag gaggatgcgg atcaagtaat gcaagcgatg
     1921 ccagttcaag tgcagcaaat tcttctgaaa aaacaagtga tggaagaaaa aatattgatt
     1981 tctggtcctt ttggggatcc ggtgcacgaa aagaagctat cgaagaaatc attgaggatt
     2041 tcaatcaatc acaaaacgag atccatgtaa attataagta tcagccatgg ggtgacatct
     2101 ggactaaatc tttatctgca ataacagctg gaaatgcacc cgatgttatt gtacaggaca
     2161 taaattccgt tgcccaaaga gcagaagcac agcaagcaac taatctctcc aaatatgtag
     2221 aaaaagacac ttctaaggat ttttatccgc agctttggga tacagtagaa tatgaaggtg
     2281 atccttatgc gatcccattc aatacagata cacaagtcat tttttataat aaaaagttat
     2341 ttaaagaagc tggaatcagt gaacaagatc taccgacaac ttgggatgaa ttagaagaaa
     2401 cagcacgtaa actagataaa aaagaaggaa aagaatttac acgcatcggt ttttatccat
     2461 tatggaattt aggggctgat gtatgggcgc taaacgcaga taacgggact agttggtttg
     2521 acaaagatga aaaagtcaag attgatacta aaaataaagt ggaagccttg gagtggatcc
     2581 aagactggca agattattat gggaaaaata cgatcaataa attagaagcc gaatttgggt
     2641 ctggtatatc tgatccattt atttccggat tagtagccat gcgtgcccaa aatatcaatt
     2701 attactctag tttgatggaa aatgcaccgg atgattttga ttttggcgtg atgcagctcc
     2761 cagaaaaaga agaaggttca ggccattggt cgtggggtgg cggattcgtc ttggaagtac
     2821 catacggagc aaaagatcct gaggcctctt atgaattcat caaatatctt tcttctccag
     2881 aagtacaaga aaaattcggc gaaaaaagtt ttgatatcat ggcgagcaag acagccaatg
     2941 agaatttagt gaataatgac aacctaacgg ataaaggaaa aatgatctat caaatggcgg
     3001 acgaaaactt taaaaatacg gtgatcacac cagttccttt agatgctccg gattacacga
     3061 cattagtgaa tgaacagatt gaccagattc ttttaggaac aaaatcacca aaagaaggat
     3121 tggcagatgc acaaaaagca gtagaaaact tagtcaagca aaatcaataa tccatttttg
     3181 actgtctagg tacagaggag gcaagaaaat ttgaagcgtt tatctatccg aaagaaaaaa
     3241 gaagcaagat gggcctattt atttatctcg ccttttatta tcgggttctt ggtatttatg
     3301 ctagggccta tgcttttttc agttattggt agtttcaccg attataattt gacatccaag
     3361 atgaatttca ttggtctagc aaatttcaaa agaatgttcg ttcacgatga tttattttgg
     3421 aaatcgttat acaacaccgt ttatttcgtc atcttcaatg taccgctgac gacgattttc
     3481 agtgtgtttt tagccacagt actcaatcaa aaaatcaaag gaatcagtgt attccgtaca
     3541 gctttctatt taccggcagt cttatcagga gtagcagtct acatcttatg gatgcagctg
     3601 ctttctccat ctgcaggatt gatcaacaca tttctgggct ggttccatat ccctggacca
     3661 gcttggctgt ttgatccaca atggacgaaa cctgcgttga ttttgatgaa aatatggagt
     3721 tctggaggag caatgctact ttacttagct acattacaaa atgttcctag atctttatat
     3781 gaatcagcag aaatcgatgg agcaggcgtt tggggaaagt tcaaaagtat tactttgcca
     3841 ttaatcactc ctattatttt ttacgatgtg atcacctcta cgatcggttc tttccaaatt
     3901 tttcaagaag cttatgtcat gacgaaaaat ggaacaggcg gacctgcaaa ttctttgctt
     3961 ttttataatc tgcatatgtg gaacaaggcg ttcgtagctt ttgatatggg ttatgcgatg
     4021 gctatgtcaa tgatcctgtt tctgatcgtg ttggttctga cgtttgtcaa tttgaagctt
     4081 acacctaagt gggtctatta ttctggaggt gacggcaaat gatggattat ttcaaaaaga
     4141 ccagtgtgaa tcggcaaaca aaaaaaatac taagttatgt cctcatgaca gtaataggaa
     4201 tcattttgat cattccattg ctttggatgg tgtttacttc attgaaacca atggaagaaa
     4261 tcgttcgtta tcctccgacc tttttcccag aaaaaatcgt atgggaaaac tatctggata
     4321 cgattgccgc ctttcctttt tggcgctatg caagaaatac attattcatc actgtgctag
     4381 tagtgatcgg taatgtcctt tcaaattctt ttattgctta tggatttgcg aagcttgatt
     4441 ttcctgggaa aaagctgatg tttgctttgg ttctttctac gatgatgatc ccggggtttg
     4501 taacgatgat ccctcaatat gtattattct ccaagatcgg ctgggtaggt acttatttgc
     4561 ccctgatcgt tccatcgttc ttcggcaatg cgttcaatat atttctgatg cgtcaatttt
     4621 acttatcgat caataatgaa ttgatcgaag ctgctgaaat cgacggagcg aatcatttgt
     4681 atatttggag tcatttgatg cttccattga cgaaacctgc cttgattacc attgcgatca
     4741 attccttcaa cgcagcttgg aatgatttct taggtcctct gttatatatc caagatcaag
     4801 aaaaatacac gttacagatt ggcttgcaag tttttcagaa ccaagcaacg acacaatgga
     4861 attatctgat ggcaggagca acacttgtct tgatcccgac gattttgctg ttcttttttg
     4921 cacaacgtta tttcattgaa gggatggatt tgacaggagg ttcaaaagga tgatgaatcg
     4981 atcgattggt aaaaaagcca atgattattt cttagaaatc attgaatgga ttccacgtct
     5041 tatccattta cagctactat ggttcctgtg cgtcttgcct gttttcacat taggaactgc
     5101 aagtcgaaca gttctgtata tgatttatca ttacaaaaaa aatgaagaga aaaagctcgg
     5161 ttcactattc tggaaaaagt ttcgtgagaa cttctctctt tatggaaagc aagatagttt
     5221 agcaagtgtt tattttcttc ttctattgat cgattatcag attttccgct attggggtgg
     5281 gggaattggc tatacattga tgtatactac gttgtttttg cttctattaa gcgcgcttct
     5341 ctttagttac aaaatcttgc ttcaactgga acaaaaagaa gcttcttgga ttactgcatt
     5401 tttcttgttt tttaatgatt tcaaaagcgc gcttttctat ttggcaggca cgttgatcct
     5461 tcttgtcgca ttctggtttg caggaccaat ctattttgct ttattaggtt tcagtttctt
     5521 gttttatttt caagtcctgc tttttatcca tcgtacgcaa gaaaaggttc ttaaaaagga
     5581 ggaataacat gttgcgtttg attgctaaaa tcgatgatag tattatcaac aagttcgcca
     5641 agcaacggaa aacggatagt cgaaaaatca attttttgaa atttatcagt aatatctcta
     5701 atggtggttt tatttggttg ctccttgctg ctgttttagt cttgatacct gatactagag
     5761 aatatggtct agccattctt atcggcagtt tgatctattt ttttgttggt ttgcttttta
     5821 tcaaaaaaac gacaagacgg aaaagacctt atgaaaaaaa tcgtcggctt gtcctttaca
     5881 caagtaaacc gaagacttct tcttttcctt caggacatac agcgctagcc tttttatcag
     5941 tagctgcgtt gttcgaatta agtccagttg caggaatatt agccttttta tttgcttgtt
     6001 tgatcgctta ttcgaggatt tttttacttt tacattatcc tactgacatt ttaggaggag
     6061 ctattttagg aagtgttatt ggcatcctat gcgctgccgt tatctgaatt aattgtacga
     6121 ataaagcatg agtgcgtaaa tgcgcatttg tgctttattt ttttgtttcg aggaaagcgt
     6181 agttgtttat tcatttgtct taataa
//
LOCUS       DAXWLG010000139         6194 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXWLG010000139 DAXWLG010000000
VERSION     DAXWLG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.141"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..704)
                     /locus_tag="ACGE4T_002207"
     CDS             complement(<1..704)
                     /locus_tag="ACGE4T_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387429.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIKNWSDYQSSDKPMTSDGQSSDKPMTTNKNDKNDKNDKNNNLRNSRKTREYADD
                     DPNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSNDF
                     WSGVILSPTSLRKHFD"
     gene            complement(722..1537)
                     /locus_tag="ACGE4T_002208"
     CDS             complement(722..1537)
                     /locus_tag="ACGE4T_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFF8387430.1"
                     /translation="MSQNLKTMSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEVFMNIYQGEKETIVTGELFGTTWKGKIDCLNVDDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLFQQYGKSFMPIIAAVSKQTPSEARLITI
                     DQDNMDYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            complement(1500..2522)
                     /locus_tag="ACGE4T_002209"
     CDS             complement(1500..2522)
                     /locus_tag="ACGE4T_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFF8387431.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTSGNLLQMAASNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLANGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKALINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKIAEPIQTEAEPIV
                     IPDDVQEGTTRVADVPGHPEIEQAHFIDKEDIEPVQEELIDIPDFGREEGVDDVSEFE
                     DDEYPF"
     gene            2576..2926
                     /locus_tag="ACGE4T_002210"
     CDS             2576..2926
                     /locus_tag="ACGE4T_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387432.1"
                     /translation="MYKLKKDNYNELSGICRSVMQNIKDLEEGSKMDLFDPRISDEEY
                     HLYSSVLKSYNSLGNQLLSNWETLKFYDIDAFFTKLSTYVEYLDIFIKEPTIENQLRL
                     TSIYDSLDKLNQRI"
     gene            complement(2910..3509)
                     /locus_tag="ACGE4T_002211"
     CDS             complement(2910..3509)
                     /locus_tag="ACGE4T_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387433.1"
                     /translation="MDKSIRIDSDIVNHIKRLNLDGFSAGRSLVGNPATIADEKTIDL
                     FKEIKKVVKENGASYDQTRKALIMVDESFYQNLLKKENPFKEKQAEPINEPKKEDINV
                     TREDLESVELTIDPDLPEKLEKELRELSKEFFNKILAAKKELLPSMKEEELVFLHKKL
                     NEFRRETPSDGFDANFHIGQILTDVILIVFAGDDLKYAD"
     gene            complement(3512..3706)
                     /locus_tag="ACGE4T_002212"
     CDS             complement(3512..3706)
                     /locus_tag="ACGE4T_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387434.1"
                     /translation="MNDFDSLGARQQLPEEFKPIGIDWQGNPLYPGDTCYLTEEGYVP
                     VDDILEYAQQHYPKIELGGI"
     gene            complement(3693..4049)
                     /locus_tag="ACGE4T_002213"
     CDS             complement(3693..4049)
                     /locus_tag="ACGE4T_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387435.1"
                     /translation="MSVKDVAKKQILKMEHIIDQINEAEDLLISLKSPALNNLNELMT
                     DISIGVPTQFLGRDFFQEEQSRWHKVQLERDLGITEIQEEIRNLVSKSVQKRIETLES
                     ELKEMIYYRGDQDERF"
     gene            complement(4060..4284)
                     /locus_tag="ACGE4T_002214"
     CDS             complement(4060..4284)
                     /locus_tag="ACGE4T_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387436.1"
                     /translation="MISVKGLGDEIFEAMMHKAQQDVQDKILTAAKYGQTSCTVRSKG
                     LTPSFLAALESEGISNIQREEGSVKLFWEF"
     gene            4436..4915
                     /locus_tag="ACGE4T_002215"
     CDS             4436..4915
                     /locus_tag="ACGE4T_002215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387437.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNTKVNVENTDRKNDESY"
     gene            complement(4974..5144)
                     /locus_tag="ACGE4T_002216"
     CDS             complement(4974..5144)
                     /locus_tag="ACGE4T_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387438.1"
                     /translation="MGKFNRALVFSTPFIIYALGLWGSRQALIGTIVYMLWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(5144..5443)
                     /locus_tag="ACGE4T_002217"
     CDS             complement(5144..5443)
                     /locus_tag="ACGE4T_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387439.1"
                     /translation="MKKPTLSELIEAAEKAVNPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKGLSPSDVLKEAS"
     gene            complement(5403..6158)
                     /locus_tag="ACGE4T_002218"
     CDS             complement(5403..6158)
                     /locus_tag="ACGE4T_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFF8387440.1"
                     /translation="MKELIKVTTNEENEQLVNGRELHEFLEVATEYKKWFSRMAEYGF
                     VENIDFVRVTQKCPTPGGIQNITDHAMKLDMAKEISMIQRSEKGKLARQYFLQVEKAW
                     NSEEMILMRSRQILERKVETLQLENEEMKPKALFADSVSASHTSILVGELAKLIKQNG
                     VDIGSKRLFSWLREKEYLIKRKGTDWNMPTQKAMDLGLFEIKETTISHSDGHISINKT
                     PKVTGKGQVYFVNKFLGGVISEKTNAFGIDRSC"
BASE COUNT         1674 a         1241 c          962 g         2317 t
ORIGIN      
        1 ttatcgaaat gctttcttaa gctagtcggt gataaaatca ctccagacca gaaatcatta
       61 ctagtggccc acacaatcat gtcttgaact tctctaccag ttcgtttgtc agattcaatt
      121 gttaaacgaa tcgtattcgc ccatttgtcc aaatcaggtt ctttgatgtt ttgatttttt
      181 cgaatgagtt ttaataaaag aatggccaat tttttatttg gatcgtcatc tgcatactct
      241 cgtgtttttc gagagttgcg aagattatta ttcttatcat tcttatcatt cttatcattc
      301 ttgtttgttg tcattggctt gtcacttgat tgtccgtcgc ttgtcattgg cttgtcactc
      361 gattgatagt cagaccaatt ttttattgtt ataacgctgt atctagcgtt cgattggatt
      421 gtcaataatt cttcgttttc aaatttctta agccatctcc atagcgttcg ccatgcaatt
      481 gctttgtcac tcggtactcc ttcgttgtac tcttttgcta ttgcatgggc tcctgtgacg
      541 aattgtccgc ttgtcaaacg tacttcttga ccgttaaata aaaattttct atcttcatga
      601 ctagccttca ttaaacacaa tatccatagc ttgaacatat cggaattggt ccaaacgaat
      661 gaactagtca ctttacggta taacttgatg tatcctagat tcattcgcaa agcacctccg
      721 tttaaagatc gtccatgcta gtgaatccag taattggaag attacctcta cagtactcgc
      781 acaaaccgca atggttcggc ttctcttcgc cgttcttcac tctgacaatt ctttcaattt
      841 tttcttttaa cataaccaac tcataatcca tattgtcctg atcaatcgtg atcagtcttg
      901 cttcgcttgg tgtctgcttg gacactgcgg cgatgatcgg cataaaactt tttccatatt
      961 gttggaaaag taattcctga taaatcgcca tttgaagcac gtaaccaaaa ttgacaatga
     1021 acgtttctct tgatccatag ttttcgttcc atttccgttc gtgcatgtct tttgttgttt
     1081 tgatgtcaac aaaatactca tcgtcaacat tcaagcaatc aatcttgcct ttccatgtcg
     1141 ttccaaatag ttccccagtc acaatcgttt ctttctcacc ttgatagatg ttcatgaaca
     1201 cttcttcttg tttaagtcgt tcaatcattt gttcagcaat ttggaaatct ttcaacaatc
     1261 catatggctt cctactagaa aacattttgg atttattttc ttctttgaac gcttcatgta
     1321 cttcttttga ttcaaaataa gaatgtacgt agtttcctac gagtaaggct ttgggatccg
     1381 acgtcggtgc ccaatcgcct ttcaactttg ctaaggctgc ggcttcacat tttaaaaagg
     1441 atttatattg tgatgttgac atgtactgcc agtcagcttc gttggagtaa taattttcat
     1501 cagaaagggt actcatcgtc ttcaaattct gagacatcat ctacaccttc ctcacgtcca
     1561 aaatctggaa tatcgatcag ttcttcttgc actggctcga tatcttcttt atcaataaaa
     1621 tgagcttgtt caatttctgg atgaccagga acatcagcaa cacgcgttgt tccttcttgt
     1681 acatcatcag gaatcacgat tggttcagct tctgtttgaa ttggttcagc aatctttttg
     1741 tttgaaaaga atttttgttc gagagtagcc actttttctg tttgtggcgt tacatctttc
     1801 cgatcattgt caaattcgtt ttctaacgta tcttttgcag cttgaactaa caaatcgtta
     1861 tcgttgcttg tgttgattaa tgctttagct gctcgattaa tcactgtccg tttagccatt
     1921 tcttctggaa attccttttg tggtccagaa cccttcattt ttgatttcgc ccaactttga
     1981 tcgatttgct ttttcgtcat gactgttgtg gtttctttac catttgctaa cttgataact
     2041 acataggctg caatgatttc gttgtctaaa ttctcaaatt ttgtttcatg ttttttgaca
     2101 accatgtttg gtccatccat tgcaacttca aattcatcgc cttgtcgaac gactacaggc
     2161 gtaatatctg cccctccagt gacacgatca aggaccgcca ttgttccgaa atatgaacgc
     2221 atgagctgaa ctttgtttcc gtatttaatg aaatagcatt gtttttttgc tggagataat
     2281 ccttgaataa ccatgtctaa aagtgcatta gaaatggatg tcttcatttc ctgattgctt
     2341 gcagccatct gcagcaaatt tccacttgta ttgttagtga gttcgaaaaa agcactcttc
     2401 aacgcgtttt gcggactata tcctggtggc atttctaagc cttgttcttg taaccgtcca
     2461 agattactga tcacctgttc atctaatgaa cgttgtgttg tttgtgttaa atcatttgcc
     2521 atattttatt ctcctttatt tttgctatac ttattttgaa agtgaggcga taaaaatgta
     2581 taaattgaaa aaagacaact ataatgagct ttctggaata tgtcgctcag taatgcagaa
     2641 cattaaagat ttagaggaag gtagtaaaat ggacttattc gatcctcgta ttagtgatga
     2701 agaatatcat ttatactcat ctgttcttaa aagctataac tctttaggaa atcagttgct
     2761 cagtaattgg gaaaccttaa aattttatga tatagacgct tttttcacta aactgagtac
     2821 gtatgtcgaa tatttggata tattcatcaa agaaccaact atagaaaatc agttgagatt
     2881 aacgtctatt tacgacagtc tcgataagct taatcagcgt atttaagatc atcacctgca
     2941 aaaacaatca gaatcacatc tgtcagaatt tgtccgatat gaaaatttgc atcgaatcca
     3001 tcggatggag tttctcttct gaattcattt aattttttgt gtaaaaaaac cagttcttct
     3061 tctttcattg aggggagaag ttcttttttt gctgccagaa ttttattgaa aaactctttg
     3121 cttagttccc gcaattcttt ttctagtttt tcaggtaaat caggatcaat tgttaactcg
     3181 acactttcta aatcttccct tgttacgttg atatcttcct ttttaggttc attaattggt
     3241 tcagcttgtt tttctttaaa tggattctct tttttcaata agttttgata aaaactttca
     3301 tctaccataa tgagggcctt tctcgtttgg tcataacttg ccccattttc ttttacaacc
     3361 ttttttattt ccttgaatag atctattgtt ttttcatcag cgatagtagc tggattccca
     3421 acaagagaac ggccagcgct aaagccatct aagtttaacc gtttaatatg attcacaata
     3481 tctgaatcaa tacgtatact tttatccatc cctaaattcc tcctaattca atttttggat
     3541 aatgttgttg cgcatactct agaatgtcgt ctactggcac atagccttct tctgttagat
     3601 agcaagtatc acctggatac aacggatttc cctgccaatc aattccaatt ggtttaaact
     3661 cttctggcag ttgttgtcta gcacctaatg aatcaaaatc gttcatcttg atcaccacga
     3721 taataaatca tttctttcag ctcactttca agtgtctcaa tgcgtttttg aactgatttg
     3781 gaaacaagat tacgaatttc ttcttgaatc tctgtgatac caagatcacg ttcaagttgg
     3841 accttatgcc acctgctttg ttcttcttga aaaaaatctc tccctaaaaa ttgagtaggc
     3901 acaccgatgg aaatatcagt cataagctca ttaagattat tcagtgctgg tgatttcaaa
     3961 ctgataagta aatcttccgc ctcgttgatt tgatcaatga tatgttccat ttttaaaatt
     4021 tgctttttag ctacatcctt tacactcatc atttgcctcc taaaattccc aaaataattt
     4081 gacacttcct tcttcacgtt ggatgtttga aatgccttcg ctttctaatg ctgctagaaa
     4141 tgatggtgtc agaccttttg aacgaacagt gcaacttgtt tgtccatact ttgctgcagt
     4201 taagattttg tcttgtacat cttgttgtgc tttgtgcatc attgcttcga atatttcatc
     4261 gcctagccct ttaactgaga tcatttattt cctcctgtgt tataatggtt taaaagattt
     4321 ttcttttgga cacgatttgc ttgccggcac tcgtgtcttt ttttattttt caaaattact
     4381 aattcttgca ttcttatttc tctccttttt gatataattc tggtggggtg aaaacatgag
     4441 cagaaaaatt gataatataa tattgttaac aactattttt tatttagtaa tcggagcaac
     4501 tctttcctta ctgtcatttt tgtccaacag tagtttgatt gtgttaaatc aaaaaagcat
     4561 attaacagaa tttgcccaca tagattactt ctcttttttt aaaactggac taatatgttt
     4621 atttctatct tcatttatcc tctatatagg aactacattt tgtctaatgg tgcttaattt
     4681 aaaaatgttt ataattgaaa aatggatagt tattatcgca aagattttct catcgatatc
     4741 aattatcatt ccaatgacat tgacgttttc taaagagcaa tttaacgtag ctactacatt
     4801 cgtatctttt atagctctat tttcgtttgc tgttcctaaa acttttcaaa gcaaatctag
     4861 caacacaaag gttaatgtag aaaacactga tagaaaaaat gatgaaagtt attaaaaaaa
     4921 ataattcttt catttattgt caaacctttc ttatagacat tagacttttt tagtcagtcc
     4981 ctcccgactg gcttttttgt tttgtactca gcttcatcaa gcccaatgaa aatccaaagc
     5041 atataaacga tcgtgccgat taatgcttgt ctactacccc atagacctaa agcgtagatg
     5101 atgaatggtg tgctgaatac taatgctctg ttgaatttac ccattagctt gcctccttca
     5161 aaacatctga aggcgataaa ccttttacac gatatttgtt tgcgtctttc cactttaaaa
     5221 accaaataaa agtatggtaa tgaataaatg tggttgagtg acctggacga agtattcctt
     5281 ctgcaaattc aggaattgtt tccatttcct tgcagtattc aactaaagtg gtttttgaca
     5341 taccgtggaa cttctccaag atcaaacttt gacgatacca atcgtctgga tttactgcct
     5401 tctcagcagc ttctatcaat tccgaaagcg ttggtttttt cacttattac ccctcctaaa
     5461 aatttattaa caaaatatac ttgtcctttg cctgtgactt ttggagtttt attaatagaa
     5521 atatgaccat cagaatgaga aattgttgtc tcttttattt caaataaacc tagatccata
     5581 gctttttgag ttggcatatt ccaatcagtt cctttacgtt tgattaaata ttccttttca
     5641 cgtaaccaac taaatagtcg tttagaacca atatcaacac cgttttgctt tatgagtttt
     5701 gctaattcac caactaaaat actcgtgtga ctcgcactaa ctgaatcagc aaataatgct
     5761 ttgggcttca tttcttcatt ttcaagttgt aacgtctcta cctttctctc gaggatttgc
     5821 cgacttcgca ttaagatcat ttcttcgcta ttccatgctt tttccacttg aagaaaatac
     5881 tgcctagcta acttgccttt ctctgatcgt tggatcattg agatttcttt tgccatgtcc
     5941 aatttcattg catggtcggt aatattctga attcctccag gggtcggaca tttttgggtc
     6001 acccttacga aatctatatt ttcaacaaaa ccatattccg ccatacgact aaaccatttt
     6061 ttgtattctg ttgccacctc caaaaactca tgcaattctc tgccgtttac taactgctca
     6121 ttctcctcat ttgttgttac tttgatcagt tctttcatgg attacactcc ttaaaaatga
     6181 ataaatatgt tttt
//
LOCUS       DAXWLG010000140         6090 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXWLG010000140 DAXWLG010000000
VERSION     DAXWLG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.142"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            61..2658
                     /gene="adhE"
                     /locus_tag="ACGE4T_002219"
                     /gene_synonym="adhC"
     CDS             61..2658
                     /gene="adhE"
                     /locus_tag="ACGE4T_002219"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFF8387441.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2887..3993
                     /locus_tag="ACGE4T_002220"
     CDS             2887..3993
                     /locus_tag="ACGE4T_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8387442.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4010..5034
                     /locus_tag="ACGE4T_002221"
                     /pseudo
     CDS             4010..5034
                     /locus_tag="ACGE4T_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5040..5519
                     /locus_tag="ACGE4T_002222"
     CDS             5040..5519
                     /locus_tag="ACGE4T_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFF8387443.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5535..>6090
                     /locus_tag="ACGE4T_002223"
     CDS             5535..>6090
                     /locus_tag="ACGE4T_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFF8387444.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         1958 a         1076 c         1372 g         1684 t
ORIGIN      
        1 attgtatgat atacatgtga attatttaac aaataaccat gacccattag gaggataatg
       61 atggctaaag taatagaaaa agaaaaaacg aagacaattg acgtacaagc aatgattgat
      121 gatttggcag aaaaagcaaa tgcagcattg aaagaaatgg aaagctttga tcaagaaaaa
      181 gtagaccata tcgtgcatga aatggcaatg gcagctttgg atcaacatat gccattagcg
      241 aagatggctg tagaggaaac aggccgcggt gtttacgaag ataaagcaat caaaaatatg
      301 tatgcgtccg aatatatttg gcataatatc aaacatgata aaacagttgg tgtgatcaat
      361 gaagatgtcc aaaaagggtt gattgaaatt gctgaaccag tcggtgtggt ttgcggagta
      421 actccaacaa cgaatccgac ttccacaacg attttcaaat caatgatcgc gttgaaaaca
      481 agaaatccaa tcgtttttgc tttccatcca agtgcccaaa aatcttctgc agaagcagca
      541 cgagtggtac gtgatgctgc tatcgctgcc ggagctcctg aaaattgtat ccaatggatc
      601 gaacatcctt cgatcgaagc gacttctatg ctaatgaacc atccaggtat cgcgatcgtc
      661 ttagcgacag gtggagctgg aatggtcaag tctgcttact ctacaggaaa accagcttta
      721 ggtgtaggtc caggtaacgt accagcttat attgaaaaaa cagcaaaaat taaacgtgca
      781 gtgaacgatt tgatcgtttc gaaaacattt gataatggaa tgatttgtgc ttctgaacaa
      841 gctgtgatcg tggataaaga aatctatgca gcagtaaaag cagaattcca agcacaccaa
      901 gtttatatcg taaaaccaga tgaattgcaa aaattagaag atgcagtgat gaacgaagga
      961 aaatatgcgg tcaatccatc aatcgttgga cattctgcga tggaaatcgc aaaattggca
     1021 ggaatcagtg taccaaaagg aacgaaaatg ctgattgctg aattggaagg cgtaggccca
     1081 gattatccgc tatcaagaga aaaactttct ccagttcttg ccatgatcaa agctaacaac
     1141 acggaccatg cgtttgattt gtgtgaaggt atgttgaatc ttggaggatt aggacataca
     1201 gcagttatcc attcagaaaa cgaagagttg catgtcaaat tcggtcttcg aatgaaagct
     1261 tgtcgtattt tagtgaatac accatctgca gaaggcggca tcggtgatat ctataacgaa
     1321 atgattccgt cattgacttt gggatgcggt tcttatggta agaactctgt ttctcgtaac
     1381 gtctctgctg taaacttaat caatgtcaaa actgtagcga aacggagaaa taacatgcaa
     1441 tggttcaaat taccgccaaa aatcttcttt gaaaagaact ctctattgta cttagagaaa
     1501 atggaaaacg ttgaacgtgt catgatcgtt tgtgaccctg gtatggtcca attcggttac
     1561 tgcgatacag ttcgtgaagt attggcacga cgtaaaaatg atgtgaagat cgaagtcttc
     1621 tcagatgttg aaccgaaccc atcgacaaat accgtatacg caggaacgaa actgatggca
     1681 gacttcaagc cagatacagt aatcgctctt ggaggcggtt ctgcaatgga cgcagctaag
     1741 ggaatgtgga tgttctatga acacccagac acatctttct ttggtgcaaa acaaaaattc
     1801 ttggatatcc gtaaacggac atataagatc gacaaaccag aaaaaacaca attcgtatgt
     1861 atcccgacaa catcagggac tggttctgaa gtaacgccat ttgcggtcat cactgacagt
     1921 gaaacacatg tgaaatatcc gttagcagat tatgcattga cgccagatgt cgcaatcgtc
     1981 gatcctcaat tcgtcatgtc agttccagca tctgtcacag cagataccgg gatggatgtg
     2041 ttaacacatg caatcgaatc ctatgtttca gtcatggctt cagattatac acgtggattg
     2101 agcctacaag caatcaagtt agtctttgac tatttagaaa aatcagtaaa aacacctgat
     2161 atggaatcac gtgaaaaaat gcacaacgct tctacaatgg ctggtatggc gtttgctaac
     2221 gcattcctag gtatttgtca ctcagtcgca cataaaatcg gcggggaata cgggatccct
     2281 catggacgga caaatgcgat tcttttaccg catattatcc gctataatgc aaaagatcca
     2341 caaaaacatg cgatgttccc taaatatgac tatttccgtg cagatacaga ttacgcagat
     2401 atcgcaaaat tcttaggtct gaaaggcgaa acaacagagg aattagtcga agctttagca
     2461 acggctgttt atgaattagg aaaatctgtc ggcatcaaca tgagtctaaa agctcaagga
     2521 gtcacacaag aaacactaga tacaaccgta gaccgtatgg ctgaattagc ttacgaagac
     2581 caatgtacga cagctaatcc gaaagaacca ttgatctctg aattgaaaca gatcatcatt
     2641 gatgcttaca acggataagc aaaagaaagc gtagaaaaaa gataagaaaa tagatgaaaa
     2701 agaacttgga aaacgttgtt tctccaagtt ctttttttaa tttccaagta actgaaagtt
     2761 acttttgtta tttaacattt aggtcaattt tagacagttt ttgttcaaaa agcgatattt
     2821 atagtggacg gtattactgc tatattaagg atataaaatg aaagcgttta aaaaggtggc
     2881 gtaaagatga ttattaaaga agagattttt gtacctaaag aattagtgaa agaaaaaatt
     2941 gacctgctcg ttgaaaacct gacaacgatc aaagacgata atggtgaatt tttgttggac
     3001 tttgatggac tgaaagtaga tgacaaaagc tggacagtgt ggaattggcc acaaggtgtc
     3061 ggtctatatg gtatttataa aaattaccag atgacaaaaa atcctcgcgc ttatcaagtg
     3121 gtcaatgaat ggtttgagga tcgtatggaa gaaggcgctc cgcccaaaaa cgtcaataca
     3181 atggcaccgc tattgactat ggcttacttg tatgaagaca cgaaagattc acgctatttg
     3241 ccttatttag aacaatgggc agaatgggtc atgaatgaca tgcctcggac aaatgaagac
     3301 gggttacagc atgccacata tggaccggaa aataagaatc agttgtggga cgatacattg
     3361 atgatgacag tgcttccttt ggcaaaaatc gggaaactgt tgaatcgccc agaatattta
     3421 gaagaagctc gttaccagtt tatgatccat atcaaatact tgatggataa acgaacagga
     3481 ttgtggtatc acggctggac gtttgaagga aatcataact atgccgaagc attttgggct
     3541 cgtggaaact gctggttgac gattgctatt ccagagatca ttgaaatctt agaattatcg
     3601 aaagaagatg cattgcgcaa attattgatc gaaacacttg aagcacaagt gcgtgcatta
     3661 aaaacttatc agtctgaaag cggtttatgg catacgttgt tggatgatcc ttcttcttac
     3721 gtagaatctt ctgcaactgc aggatttgct tacggtattt tgaaagcggt ccataaacgt
     3781 tatttaccac aagaatataa agaagtcgct tatcgtgcta tccaaggatt gcttgaacaa
     3841 atcgatgaaa aaggagaagt tcaaaacgtt tcaatcggga caggaatggg ggacagttta
     3901 gatttttatc ggaatatcgg aatcacagca atgccttatg gacagtcgtt aacggtgctt
     3961 tgtttgacag aattgctcgt ctcttattgt taaaagaaag gaaacccata tgaaaaaaac
     4021 atcactgctg tttgcagcaa gttcgcttag cctaggtctg ctaggtctaa gcggctgcgg
     4081 aaataattct agtgtggcaa ctgtcgcaga tgcagacgta aatgagatag ttaccttgcg
     4141 ctttgcttat gcaagcaaca gccagtaatt gatgcaatga atgaatttgg gcgtttagta
     4201 aaagaaaaaa cagatggttc cgtacagatc cagtatttcc cagatggaca actgggtgga
     4261 gaacgtgaac tgatcgaatt gacacagtca ggagcagtag atttcacaaa agtaagtgcc
     4321 tcagcgttgg aaagtttctc taaagattat tcgatttttt ccgttcctta tctattcaat
     4381 gatgaagaac atttctttaa ggtgatggat aatgaagaaa taatggagcc aatctaccaa
     4441 tcgaccaaaa atttaggatt taccggattg acctattatg attctggtca gcgcagcttt
     4501 tatatggtag acggtccgat ccatacgccg gaagatttga agggaaagaa gatccgtgtg
     4561 atgcaaagcg aaaccgctat caaaatggta gagcttttag gcggttcgcc cgtacctatg
     4621 ggaagtgatg aagtgtacac gtctcttcaa tccaatttga tcaacggatc agaaaacaat
     4681 gaatttgtct tgtatacagc tggacatggc ggggtagcta aatactattc ctatgatgag
     4741 catacacgtg tgccagatat catcatcatg aatgatgcca tcaaagaaca gctaactgat
     4801 gaacaggaaa aagccattga agaagcagcg aaagaatcaa cggtttttga aattgaagct
     4861 tttgcaaaag caattgaaga agaaaaagct ttagcacaag aagaacatgg agtccaattc
     4921 aatgatgtag ataatacacc gtttcgagag gcagttgccc cacttcatga tatattcaaa
     4981 aacgatccta cctacaaaga cctttatcaa aagatccaag agcaaggagc gtagctgtaa
     5041 tgaaaatgat tttaaacaag gtattagaga tcctgggagc tgttttgctt gttgggatgg
     5101 tcgctgttgt tttatggcaa gtattttcca gaacggtttt aggaaatcca aatacggtca
     5161 cggaagagct tgttcgattc ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg
     5221 tgggacaaaa agcacgttta gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa
     5281 gtaaaatttt agaagttgtt gttcaaagtt tatttctggt atttgctgcg atgatcatgg
     5341 tgtatggcgg atggaatgct gtcacattga caatggggca gatctcgcct tctttaagta
     5401 ttcctatggg atatgtttat ctgtcagtac ctgtatcggg ggtactcatc attatctata
     5461 gcctgatcaa tctaatcgat acattaagca acaagccagc aattcaagaa aaaatataaa
     5521 aatggaggaa taaaatggca atagaagccg gattagtgtt attttttgta tttgttgtgt
     5581 tgcttgtatt agggatgccg attgctataa gtattgcatt ctcatcgatg atgactttat
     5641 tgttagttat accgtttgat gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta
     5701 tcaatagttt ttcgctgatc gctattccgt ttttcgttct ctcgggcatt atcatgaata
     5761 atggagggat agctaagaaa ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg
     5821 gggctttggc tcatacgaat gtgttaggga atgctttatt tggttccatc tctagctcgg
     5881 ccattgccgc ttctaccgcg attggtggag tgttgatccc tcagcaagtg gaagaaggat
     5941 atgaccggaa gtttgcgaca gcggtcaata tcgcgtcagc tccgacaggt atggtcattc
     6001 ctccaagtac tgcttttatc atgtattcac ttgttgctgg tggtgcttcg atctctgctt
     6061 tgtttatggg aggttatctc gtaggcgctt
//
LOCUS       DAXWLG010000141         6041 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXWLG010000141 DAXWLG010000000
VERSION     DAXWLG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.143"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..1290)
                     /locus_tag="ACGE4T_002224"
     CDS             complement(<1..1290)
                     /locus_tag="ACGE4T_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387445.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDP"
     gene            complement(1301..1969)
                     /locus_tag="ACGE4T_002225"
     CDS             complement(1301..1969)
                     /locus_tag="ACGE4T_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8387446.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2099..2683)
                     /locus_tag="ACGE4T_002226"
     CDS             complement(2099..2683)
                     /locus_tag="ACGE4T_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8387447.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3014..3247)
                     /locus_tag="ACGE4T_002227"
     CDS             complement(3014..3247)
                     /locus_tag="ACGE4T_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8387448.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3262..4212)
                     /locus_tag="ACGE4T_002228"
     CDS             complement(3262..4212)
                     /locus_tag="ACGE4T_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFF8387449.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4209..5051)
                     /locus_tag="ACGE4T_002229"
     CDS             complement(4209..5051)
                     /locus_tag="ACGE4T_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFF8387450.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5053..5772)
                     /locus_tag="ACGE4T_002230"
     CDS             complement(5053..5772)
                     /locus_tag="ACGE4T_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8387451.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         2043 a         1081 c          752 g         2165 t
ORIGIN      
        1 cggatcatga gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc
       61 aatcctttcc atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt
      121 agtttttgac aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc
      181 aaacggtaat tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg
      241 atctttacta actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa
      301 ttggtaatcc atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat
      361 tatctttcct ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc
      421 cacaccgttt ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc
      481 ttttagaata ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc
      541 gatataggga acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt
      601 ttcagttttt aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac
      661 ttgatcaatt aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc
      721 tttatttttt aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt
      781 tgaagacggt tcatccaata taattaattt agagtttagc gataaactac atccaataga
      841 aattttctgc cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag
      901 atctaataat tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa
      961 gttttccatc gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg
     1021 atcttgactt acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga
     1081 gttattttca aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc
     1141 attcattaac tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag
     1201 actgcctttt tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata
     1261 tgaaaaagaa acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga
     1321 aaaaaatagt aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt
     1381 aagaacttct tttcacagga gattctatcc cctttgttat tgcattagca gtcatttctt
     1441 cagcaatcat agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag
     1501 gctgaaaaaa agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt
     1561 cagaacgata agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg
     1621 gtaaatgtat tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca
     1681 accatgagat cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa
     1741 atatagtaaa aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg
     1801 aataaatcaa gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg
     1861 tagcaataat gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg
     1921 gaaaaaaaat atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc
     1981 caaaaaataa gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac
     2041 agtgcgatct tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt
     2101 aactcagtaa ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca
     2161 tagtcttttc ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt
     2221 aaatgtagaa gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag
     2281 tctttaactt atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa
     2341 caatatcttg atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat
     2401 tctcaaaaat ataattaata aatggatcta attcattgaa gttctctctc caacttttct
     2461 ctatatctat attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa
     2521 taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat
     2581 gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt
     2641 gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta
     2701 taatataatc tctggtttct atgggtactt cctcctaata agatgaatta actttattat
     2761 cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc
     2821 taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag
     2881 actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat
     2941 ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta
     3001 aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat
     3061 cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac
     3121 ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt
     3181 ttctttttga taattcctga attgctagta aataaattag tatagtagtc gttttaattt
     3241 gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca
     3301 tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa
     3361 aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc
     3421 cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg
     3481 ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg
     3541 cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt
     3601 atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc
     3661 aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa
     3721 ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg
     3781 atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc
     3841 tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc
     3901 atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt
     3961 ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact
     4021 gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt
     4081 aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga
     4141 acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca
     4201 ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga
     4261 aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta
     4321 aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata
     4381 aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac
     4441 attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat
     4501 agaaccatca atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat
     4561 ggatcaaaag aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa
     4621 accgaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat
     4681 aaaatatgaa ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct
     4741 aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt
     4801 ataaaagtaa taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta
     4861 atacctaaaa tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc
     4921 ataagagaca ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata
     4981 actacagatg taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa
     5041 ttttgaagca tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa
     5101 attttcttca ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag
     5161 aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt
     5221 ttttccctga tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc
     5281 tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc
     5341 taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt
     5401 tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat ttttaattct
     5461 ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta cttttattgg
     5521 aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt
     5581 gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt
     5641 aagcaatgtg gattttccag caccatttgg accaataata cctgttaaag caccttcttg
     5701 tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt
     5761 cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac
     5821 aaaaattata ttttagtcaa ccctaaattt tataatttag atttttttct gttgctgaag
     5881 attagaatga caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa
     5941 aaaaggagcg ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa
     6001 attatacttg aagtggatag cgggttgaat aagttgataa g
//
LOCUS       DAXWLG010000142         6037 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXWLG010000142 DAXWLG010000000
VERSION     DAXWLG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.144"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(312..602)
                     /locus_tag="ACGE4T_002231"
     CDS             complement(312..602)
                     /locus_tag="ACGE4T_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFF8387452.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(738..917)
                     /locus_tag="ACGE4T_002232"
     CDS             complement(738..917)
                     /locus_tag="ACGE4T_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387453.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(914..1264)
                     /locus_tag="ACGE4T_002233"
     CDS             complement(914..1264)
                     /locus_tag="ACGE4T_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387454.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1257..2582)
                     /locus_tag="ACGE4T_002234"
     CDS             complement(1257..2582)
                     /locus_tag="ACGE4T_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8387455.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            3032..3646
                     /locus_tag="ACGE4T_002235"
     CDS             3032..3646
                     /locus_tag="ACGE4T_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8387456.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3811..4053)
                     /locus_tag="ACGE4T_002236"
                     /pseudo
     CDS             complement(3811..4053)
                     /locus_tag="ACGE4T_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4257..5576)
                     /locus_tag="ACGE4T_002237"
     CDS             complement(4257..5576)
                     /locus_tag="ACGE4T_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFF8387457.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(5731..6037)
                     /locus_tag="ACGE4T_002238"
                     /pseudo
     CDS             complement(5731..6037)
                     /locus_tag="ACGE4T_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1860 a         1112 c          993 g         2072 t
ORIGIN      
        1 attgcaatat cgtatatgca ttatattgta atattgtgtg attattttgt tttaaagata
       61 tatatcggtt cggatataaa gattattcat atgttattcg aaattaaaat aatcactttt
      121 tattacttta aaatttttta ttaatgataa gaaatatgtt ttgcgagcag caactagaat
      181 aatttaaaca ttactaaact gtctatattg ttactaataa ttcccttctt atagtgcatt
      241 ttcttagaaa aaaacaatat tacgatatca tgatattaag atatcgtaat attatacaat
      301 tgtgattgat attatatatg tttttttatt tttttatcat gtttaatctt gaaggaatca
      361 tattcttttt ctaaagacag gagcttttca taaacatcag acatgtttaa actagaggcc
      421 aggcttaaat cataaacgaa tgaagcaggt aatgatttta ttctacgttg tcgggttatc
      481 cagcttgcaa ccgttccttg tgggtaggca tataactcac aaaattgttc tacagtgatt
      541 cctaaattct tgataatgta aacattgatc gggtgcgagt aaacaaatgt attgatagac
      601 atggtgcgta agcctacttt cttttattat tgcgtttgca ataatagttt aacacacgca
      661 ttattctagt atctactcct atttgtaaaa acaaaaaagg attcatagag ataactctat
      721 agaatccgta aaatacctta tatatataac gggaagatat aaaacctaaa attatgataa
      781 cctgctgcag cttctacata gaaagagaag cgctcaacac caacctcatt tatcaaacgt
      841 tggccataac cccaaatctc ttctgatagc tgatcaaagg ttatatactt tagctcatat
      901 tgctggataa tgttcattct aatcgactcc atttattatc ctggtaaaat tcgatatgac
      961 gtatttcgtc gtagtgaatt ttgtcttttc caacgtagat ccccagctca tcactaccta
     1021 agatcttgcc aatgatatct tctggatagt atccttcagc gtttcgttct tctttttgaa
     1081 tagatactga tctatttttt atgactgcag cgttcaatac ttccgcaatc tcttcagtcg
     1141 tcatttgttc ttttgctaaa ttttgactgg ttcgttgttc attgtcggca tcaatttctt
     1201 cagtatgatc agataaataa aaccctgccc actttaaacg tccacgatca ttgtaatcaa
     1261 tcatttttaa ttcccccatt tccgcccgca tgaccaccaa caagtgtgct tctagtaatg
     1321 cttctagcgc cttctaacat tgaagaagca tgtacaatcg acgtaaaacc atatttttgc
     1381 cgaatcttgt caataatttt atctaaattt tcttcgtcaa tttgcttttg tggatcacta
     1441 aataaatcca gctgaagcga atcggtgtac acaagcttgc tataggttat tccaacatgc
     1501 cttacttctt gtccttcata atacttatca aacaagaaaa gacaataatt ctgcaattcc
     1561 tttgtattat ctgtgatagg gatcttcatc tggtgtgaaa accctggtct cgtttctaag
     1621 atcgacgtgc caatgtgtaa atggacacaa cctgttttac agttatgctg cctaatgcgt
     1681 atcgctactt gttccgccat ttcttttacc accagctcga tttcatttct tctagtataa
     1741 tctcgtggta acacttgtga atttccatat gatttttcct ttgtcggttc tggtgggatt
     1801 gctatatctg ttcgatcgat cccattagca tggaagtaca gctgtaagcc aatcacacct
     1861 agtctattct taatcgtata aggattccag ttagcaagat ctctaatgct cataatcccc
     1921 atctgattga gtcttttttt cattcgagag ccaattcccc aaaagtccgt catttcagga
     1981 atattccata ctttttcagg aacgttttcg tacgtccatt ccgcaacaaa tccttcatta
     2041 tgctttgctt cgttgtctaa ggctagttta gccaaaaggg gattatctcc gacacctact
     2101 gtagcaatca atcctagctc ttctttaata ttctcctgga tcatttgagc cagcttcttt
     2161 ctccgttgac ttcgtgttcc ttcagtcgta aaaagattca gtgatcgggt cacttttaag
     2221 attgattcat cgatcgagta aatcagtaga tcttcatcag ccacatatct tctgaaaata
     2281 ttatttacct gcatatttcg cttgatatat aggttcatac gtggtggaac aacgtgtagt
     2341 gttttaggaa atggttgtgg taagtcacgt ggtctactca catttgtaat accataccgc
     2401 ttttttgctt caggagaaga agctaatatc aatcctgaac cagtattatc agctcgactc
     2461 ataacaacaa gttctgttgt taatggatct aaatttcttt ctatacattc gacacttgcg
     2521 taaaagcttt tcatatcgat taaaaagtaa tcatttactg gctcttttga ataatccatc
     2581 ataaataaca ctccattctt tcacattata caaacatatg ttcgatattg taaagaagaa
     2641 atagctaaat acataaaaaa agaagcatag gtatgctatc accctgcttc ttcttttgat
     2701 ttgtatgatc aatttattct atcactttac taattaattt gaaaatctaa taaaaactct
     2761 cactctaaaa gagtgagaga ctgcaaatta gagtacggac ttttttggag ttccgttctt
     2821 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
     2881 tacaaaaaat tctcatacta tttcctccat cattagtttt tgtctatata aggtttaccc
     2941 taaatagaca ctggtataac attgaaaaat atggcttcct cgtgtatgat tagggtgtat
     3001 tttaaatagt tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc
     3061 gatcgatcaa aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat
     3121 tttcatcgag aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt
     3181 agattacgtc cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa
     3241 ttatgacgag atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat
     3301 tactagttta ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa
     3361 ggatctgatc atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg
     3421 tcgccaggca caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa
     3481 attatacagt gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga
     3541 agatcttaaa aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca
     3601 aaccgtgtat agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct
     3661 tttattatct tctcatcttt aatttcctac atataattac tatctataaa attaagtctt
     3721 actcagaaca gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga
     3781 gtctgttaat ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt
     3841 atctatattt tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta
     3901 tgtcgacaca ttggttatac taattcctat agctagttct tgagactcta cttttctctc
     3961 tagctccttt gacaaagtga tttgagattt atttggttcc gttggagtca attctataga
     4021 ttcttcataa aaatctctat acataaagct atccctgaat aattcatagc tttaattctt
     4081 tggcacattt tattgaaata tgtatcacta atatccctat caactgattt tgactaaaaa
     4141 gttacttcaa acattcactg ttcaaaaata ttgaggaata aattttgggt gtaaggcatt
     4201 ttgagcatac ttctccctgg ataacttccc agtaaaaaaa tcgttagatt gttggattag
     4261 atccattgac tggctcgcct caggcgagca aagaccctgt agaattcatt ctgatacaca
     4321 tgataactag ataatttgag atagactcgt ctacccgttt ggactaattt cccagcaact
     4381 ttcagaaact tcaatcgaat agaatgaatg gtcattccct tttggacttc ttcaaagcca
     4441 atcgttctta agaagttgac taagttgtaa gctatcaagc tgagcatcat tcggacatga
     4501 ttctccaaaa agcgaggact gtctgttttg tcaaagtaaa agccagcttt cgcttcttta
     4561 atgaagttct ccattgtgcc acgtttggca tagatagaaa agatggtatc aggagaaaca
     4621 ttttctgata gattcgtcac gataaattca tgtcgaaaga gtagttcgcc cgcttcacgt
     4681 gttgagcgta tacacacgcg acgactttgt gaccaggatt gtgcttgata agtggtcgag
     4741 aagtactgaa cttctcgttc ttcccacttt tgattatcgc cataaagtac tgatttctca
     4801 gctatttgac ctagtctacg attattcttc agtcgaataa cataatgact cttttttgat
     4861 tcacacgaat catacacacc aggtgtagcg aacccgctgt ctccacgaac caagatgtca
     4921 gtgtttggta gagagtgatt atagtgctct aataaaggtg tgagaaactc ctttacgcct
     4981 tttgatgtat attgatttcc tgaacgtagt tcagctttta agaagtcacc agtcaatccg
     5041 tcaaaagcta ccaatggatg gtaaccatag gtttggtagt gtgtattata attcgtttgt
     5101 tcttggtgac caaatgtatc tgaatgggtc gaatctaaat caatgattaa ttccgtatca
     5161 ttacggatga ggcgtgcttt atcgataagt tcttggttca aagcttgaag ttcatggata
     5221 ttctcctcag atagtcgatc taaaaatcgg gaaagtgaag attgagaagc gagttctttc
     5281 ctatctaaaa cagctttaaa cacaggatct tgtctcagga gattagctgc agaatcagct
     5341 gagtaaccag caattaattg cataatgaac tgctcaagta tggataagtt gtcatgagta
     5401 aaatacgctc gttcgtcttc aatgtgaatg tgttgcttag ccagttcaga aaaaccaaag
     5461 atgttcatca gctctttcac taggacgaga cccgaatcac tcgataattg accacctgta
     5521 tgagaaatag tgatatttga attgaatttt acttggtttt tgtgtaagct agtcatgaaa
     5581 agaactcctt tcttttggtt ggtttagtca ctttaaccat agcagaaagg ggttcttttt
     5641 tcatcactta acgggtgaag atgaaaaagt aattgtaagc tgccgtgagg cagtttcaca
     5701 tggttttaat taaaagtatg aattattcag gctatcatta attttataaa aaaaattaaa
     5761 tgtttttagc actgccatat aacattcaaa acattcttct atagttggtt tacttccttg
     5821 aggagaatga accaatttat tacgtttttc tatgaaattt ttaatttcct ttcttatttc
     5881 tttcggaaac tcaatattaa agtacccttt aaacatgctc cctaataatt tgtctacagg
     5941 gggagattgt aaattctcaa atattagagt taaatcaggt ttctcttgtt gataaaattc
     6001 ctttacccct atttcacagg ctgtaattat cgaaata
//
LOCUS       DAXWLG010000143         5970 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXWLG010000143 DAXWLG010000000
VERSION     DAXWLG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.145"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            196..921
                     /locus_tag="ACGE4T_002239"
     CDS             196..921
                     /locus_tag="ACGE4T_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387458.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACGE4T_002240"
     CDS             911..1420
                     /locus_tag="ACGE4T_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387459.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACGE4T_002241"
     CDS             complement(1490..2938)
                     /locus_tag="ACGE4T_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8387460.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACGE4T_002242"
     CDS             complement(2938..3654)
                     /locus_tag="ACGE4T_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFF8387461.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACGE4T_002243"
     CDS             complement(3635..3985)
                     /locus_tag="ACGE4T_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFF8387462.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACGE4T_002244"
     CDS             4129..4902
                     /locus_tag="ACGE4T_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8387463.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACGE4T_002245"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACGE4T_002245"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5682..>5970
                     /locus_tag="ACGE4T_002246"
     CDS             5682..>5970
                     /locus_tag="ACGE4T_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8387464.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDVVTDVPDARSDHRRYDYIKYTFVDPE"
BASE COUNT         1729 a         1170 c         1038 g         2033 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccattgt gagatttcgc cagatttcgc
     5461 tagatgctta aatccttatt ttataagggt ttgagcattt tttgttttcg ctgattttcg
     5521 ttaaatgaat gaaaatggca ttcaaaatgg cattcaaaat ggcattcaaa atggcattca
     5581 aaaaaacatc ttttaatata tatgttccat ccatattgcc aacgctattc ctgctttaca
     5641 aaaagaacat tagttcgtat actctttttg aggtgaacat tatgttgatg gaaggaaaga
     5701 cacaattatg gtttaaattt gatccttcga atagatttat caaagatttt tataaagtat
     5761 gggattcaga agttttcttt ttagcaatcg aagatagctt attaatcaat ctctactatt
     5821 ctaataagaa ctatttcaaa attcctgctg cgaaaactag aatgaagaaa gacgtatatt
     5881 ttttgtttga tgtcgtgact gacgtgccag acgcacgaag cgatcatcgg cgttatgact
     5941 atataaagta tactttcgtt gatccagaaa
//
LOCUS       DAXWLG010000144         5964 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXWLG010000144 DAXWLG010000000
VERSION     DAXWLG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.146"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            21..719
                     /locus_tag="ACGE4T_002247"
     CDS             21..719
                     /locus_tag="ACGE4T_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8387465.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(887..3526)
                     /locus_tag="ACGE4T_002248"
     CDS             complement(887..3526)
                     /locus_tag="ACGE4T_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8387466.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4145..5176)
                     /gene="queA"
                     /locus_tag="ACGE4T_002249"
     CDS             complement(4145..5176)
                     /gene="queA"
                     /locus_tag="ACGE4T_002249"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFF8387467.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            5369..>5964
                     /locus_tag="ACGE4T_002250"
     CDS             5369..>5964
                     /locus_tag="ACGE4T_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387468.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         1705 a         1298 c         1134 g         1827 t
ORIGIN      
        1 aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga
       61 gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta
      121 ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg
      181 gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg
      241 gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg
      301 atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca
      361 aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat
      421 tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct
      481 attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga
      541 tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt
      601 catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca
      661 gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa
      721 agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta
      781 aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct
      841 ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt
      901 ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata
      961 aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg
     1021 taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca
     1081 aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca
     1141 catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac
     1201 ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg
     1261 ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga
     1321 tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc
     1381 ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg
     1441 taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat
     1501 tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac
     1561 tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt
     1621 gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg
     1681 gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt
     1741 tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac
     1801 gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg
     1861 gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag
     1921 tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg
     1981 gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt
     2041 cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca
     2101 gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa
     2161 agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt
     2221 ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat
     2281 atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta
     2341 cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg
     2401 tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat
     2461 tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt tgtgtcagcg
     2521 ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact
     2581 gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa
     2641 caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca
     2701 tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta
     2761 aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt
     2821 gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg
     2881 cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa
     2941 gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt
     3001 cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg
     3061 catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact accagttgct
     3121 ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat
     3181 cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt
     3241 tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa
     3301 atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca
     3361 agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc
     3421 cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt
     3481 ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt
     3541 aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc
     3601 aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg
     3661 caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga
     3721 cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca
     3781 cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc
     3841 acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg
     3901 aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt
     3961 tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat
     4021 ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt
     4081 tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat
     4141 aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg
     4201 catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta
     4261 aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc
     4321 caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg
     4381 ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat
     4441 ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat
     4501 gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat
     4561 gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg
     4621 taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt
     4681 gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca
     4741 atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt
     4801 gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc
     4861 caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg
     4921 ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag
     4981 gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt
     5041 catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat
     5101 gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat
     5161 cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt
     5221 ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa
     5281 aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt
     5341 cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta
     5401 taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat
     5461 ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat
     5521 gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc
     5581 agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat
     5641 gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg
     5701 gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg
     5761 ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc
     5821 agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg
     5881 tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt
     5941 tgtaacccat gacatggatg aagc
//
LOCUS       DAXWLG010000145         5958 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXWLG010000145 DAXWLG010000000
VERSION     DAXWLG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.147"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            3..848
                     /locus_tag="ACGE4T_002251"
     CDS             3..848
                     /locus_tag="ACGE4T_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8387469.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1039..2226
                     /locus_tag="ACGE4T_002252"
     CDS             1039..2226
                     /locus_tag="ACGE4T_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8387470.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2232..2588
                     /locus_tag="ACGE4T_002253"
     CDS             2232..2588
                     /locus_tag="ACGE4T_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFF8387471.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2590..2928
                     /locus_tag="ACGE4T_002254"
     CDS             2590..2928
                     /locus_tag="ACGE4T_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFF8387472.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    3004..3260
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3323..4357
                     /locus_tag="ACGE4T_002255"
     CDS             3323..4357
                     /locus_tag="ACGE4T_002255"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8387473.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4350..5036
                     /locus_tag="ACGE4T_002256"
     CDS             4350..5036
                     /locus_tag="ACGE4T_002256"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8387474.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5060..5908
                     /locus_tag="ACGE4T_002257"
     CDS             5060..5908
                     /locus_tag="ACGE4T_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8387475.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         1920 a         1090 c         1339 g         1609 t
ORIGIN      
        1 ccatgtttaa gttattaacg acttttcgag cagtatatga aacaagaaat ttttccagag
       61 cagcagaagt actgttttta tctcaaccag cagtctcgaa tcaaatcaaa cagttggagc
      121 gggaattgaa tatcgatctt tttcaacgta acggaagaaa agagatgctt ccaactaagc
      181 aagccgactt attatatgaa cggacactgg ttttgttaga agaatgggaa gaaactcagc
      241 aaaaattgct caatgaacaa aatgaagaag aagtatgtcg gatcgttgct tcccacacct
      301 ttgctgtcta tcttttgcct cagttgatga aacatctgat caaaaggttt cctaaagtgc
      361 attttgagat cgaaatcacg aattctcatg aatcattaga acaagtagcg aaacatgaat
      421 ccgattttgg ttttatcgaa aagccattag agacttccgg cgtacaaaga tactctttga
      481 tggaagacca gctagtcatc gctggggacc caaaaagcaa attgtggctt gtaagagaag
      541 caaactctgg tgtttttcat tacacgcagc gttattttga agagatgaat atccaagata
      601 agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt gctgaaggaa ggaatcggtc
      661 gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc aatgactccg ttgaatttcc
      721 atcgatattt ttatttcatc caacgaacca atctggtttc tgatcagctg ctagagattg
      781 ccgaagagat ccttcgatac aatgaaagca cagaaacttc tcacagcgag gagaatctca
      841 gctggtagat aattgcatgt atcaaaaaaa gttctatgat acactaaaga taagcagtag
      901 gcttttgtac tgcatttgct caagcaactg cacaaacgga aagttaattt ttcccaaatg
      961 tctatggaaa gactttccat tatctaactg gaccgctata ataatcagca gtatggtttt
     1021 taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa tcgtattgat
     1081 tatggcgtga ttttaccagt ctttctgtta tgtttgattg gtctgctctc attatatgta
     1141 gcacttagcc atgatccgaa caatccagca gtcttgaaag gcgtagggat gcaagttgtc
     1201 tggtatttag ttggagcggc agcaatcgtt gtcatcatgc atttgaattc caaatggatc
     1261 tggaaactga ctccttacct ttatggtttg gggttagtgg taatgggact cttgctcaaa
     1321 ttctatgatc agaatttagc tgcttcaaca gggtctaaaa actggtttcg attcggtgcg
     1381 tttactttcc agccagcaga gttgatgaag attgcgtaca tcttgatgat ggcactgatc
     1441 gtaactaaac ataatacgca agtaaaagaa cggacgatga aatcggactt ttggctgatt
     1501 gggaagctct tgatcgtgac aataccggtt ttagcattga tcatggccca agatgacttt
     1561 ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt tcctgatgtc tggaatctct
     1621 tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg aacgatcttc
     1681 ttggttacga ctgaaggcgg aagagacttg ctttataaag ttggattcaa atcttatcag
     1741 tttgcacgta ttgattcatg gctggaccct ttccatgata cgtcaggaat gagttatcag
     1801 ccggcccaag gacttttggc aattggtacc ggtggcttgt ttggtaaagg attcaacgta
     1861 agcaatatct atgtgcctgt tcgtgagtcc gatatgattt ttaccgttat cggtgaaaac
     1921 ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt ttattttaat ctatcgtatg
     1981 attcgggtgt gtttcgatac gaataatgaa ttttacgcct atatcgcaag cggtttgatc
     2041 atgatgctgc tgttccatgt ctttgagaat attggagcaa atatcggatt acttccttta
     2101 acagggatcc cattgccttt cattagtcag ggaggatcct cgattttagg gaacatgatc
     2161 ggtatcgggt tgatattatc gatgagatac caaaatgaag cacctgcaag atcttcaagg
     2221 aggtaagaaa attgtacacc ttttactggt acccgaaatg ttcaacatgc aaaaaagcca
     2281 aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt tgatatgatc aaagaagtac
     2341 cgaaggcctc aacgattgag acttggctag aaacgagtca agtaccaatc cgtcgcctgt
     2401 tcaacacaag cgggatgaaa tatcgggaac ttggactgaa agatcaagta ggcgagttta
     2461 cggcttcaaa agcgagccaa gtcttatcga cagatggtat gctgatcaag cgaccactga
     2521 tcttacgaga ggaccagctt gttgcaattg gattcaatga aaaaatttat gaaggagaac
     2581 tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca acctttggat cttatttaat
     2641 ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag aagaacttgg agacatcacg
     2701 tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct gatcgaagtt
     2761 gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt ttcttctgtt
     2821 aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat gaatgcgtgg
     2881 atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg aattataaaa tcagaaaatc
     2941 acttgaaaaa agttgacact gattcttcca gttgctataa tcataagcaa tcctatatga
     3001 aacgagaaag ctttgaaaag aagagtacat gtcacggacg tttgaagaga gccctgtttg
     3061 ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta gagcagagta agcgagcgta
     3121 agcaaactta ctacgggagt gcccgttaca gcgcgacagt atacggaaag aagctaagtc
     3181 caagtactgg aagaggctgt tattgcgaag taacggtaaa tcaaggtggt aacacggaag
     3241 ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag gcttttttgt ttgaaaaatc
     3301 ataagagaaa gatgtgaatg gcatgtcatt aatccaacta acgaatgtca aaaagacatt
     3361 caatgggaaa aacggaaaca tccaagccgt caatgacgtt tcactaaacg ttgaaaaagg
     3421 ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa agtacattag tccgtctcct
     3481 gaatggactt gagcttccca cgagcggcga agtgatcgta aacgatcaag atattacaaa
     3541 attaaaaaac aaagaattac gtacattccg aaagaaaatc ggtatgatct tccagcattt
     3601 caacctgtta tggtcaagaa ctgtacttga aaatatccag ctgccgctgg aattagctgg
     3661 cgtaccaaag agcaaacgta aagaacgggc agaagaactg ttgcgtttag tcgggttaga
     3721 aggacgcgga caagcatatc cttcacaact ttccggcgga cagaaacaac gcgtcggtat
     3781 cgcacgagca ttggcaaatg atcctgagat cctgctttgt gatgaggcta ctagcgcatt
     3841 ggacccgcaa acaacagaag aagtattaga cttattgtta gcgatcaata agaaattgaa
     3901 tttgaccatc gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct gcaacaaagt
     3961 cgctgtgatg gagttgggga aagtcgtaga agaaggagac gtattgactg tcttcagaca
     4021 accgaaaaaa gaagtaacca aacgctttgt acaacaagac atcgaaccag aagctgattc
     4081 gacagaacta ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg caactttgac
     4141 ctttagcgaa agaaatgcca acgagccagt catttcccaa gcgattcgac gctataacat
     4201 cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct ttggttcatt
     4261 gactgtcttg atgactggtg aaaccaaaga attagatcaa gcagaagtgt tcatcaaaga
     4321 acaaggagta ggaattgagg tgatccatcg tggataaaag cttttcagaa acatatctag
     4381 attttaaaca aatcaacccg gaaacgatga atcaggctat ccttgataca ctatatatga
     4441 cattaatctc aatggtctta gtgacgatca tcggattagt tttgggattg atcctttact
     4501 ctattggacg caaaaacaaa ccgtatgctc gaatcattta tggaatcgta tcgatcatca
     4561 gtaatatttt ccggtcaacg ccatttatga tcctgatggt tttgatcatt ccatttacta
     4621 aagcaatcgt aggcactatg ctaggtgcgc aagcagccat tccagctctt gttttatccg
     4681 cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc cctggtgtac
     4741 tggaagcagc agatgcaatg ggagcaagtt actgggaaat catctggaag gtcttgatac
     4801 ctgaaagtat tcccgcattg atttctggat taacagttac aacgatctcg atgatcggat
     4861 ttactgcgat ggccggcgcg atcggcgcag gtggacttgg aggtcttgct tggcaggaag
     4921 gctaccagcg tggtaatcta acagtgacat tcgttgcgac tttaattatt ttactaatcg
     4981 tatttatcgt tcaaggaatc ggtgatttct taactaagaa aacagacaaa agataatagg
     5041 atataggggg atgtaggata tgaaaaagaa aatttttgga tttgcagcag cgcttttgct
     5101 gactgtagga ttagcagcat gcggtaacaa cagcgacagc aaagattcag caaataaagc
     5161 agacgacacg acattgaaag tcggcgcttc accgacaccg cacgcagaga ttttggaaca
     5221 tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat tcgatgacta
     5281 tgtattaccg aaccaatctt tggaagaagg cgacattgat gccaattact tccagcatat
     5341 tccttatttg aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg ccggtgctgt
     5401 ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg aattaaaaga
     5461 tggtgcaaca atcattactt caaattctga atcagactgg ggacgcatca tcacaattct
     5521 acaagatgct ggcttagtca aagtcaaaga tggagtagat ctggaaacag caacattcga
     5581 tgatattgaa gaaaacccta agaacttgaa gttcaaccat acgatcgacc catcactttt
     5641 agcttctacg taccaaaatg atgaaggtga cctagttgca atcaacgcaa acttcgctta
     5701 cggcgcaggg ttgaatccat tagaagatgc cgtattgctt gaaaaggaca actctcctta
     5761 cgtgaatatt attgcgactc gtaaaggtga tgaagacagt gacaaaatca aaaaactgat
     5821 tgaagtgctt caccgcaaag aagtccaaga atggatcaca gacaaatggg gcggctctgt
     5881 taaaccagta aaggctgatg caaaataagg tatggaacaa aaaagatgag aaataagtct
     5941 gtaatggctt atttctca
//
LOCUS       DAXWLG010000146         5822 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXWLG010000146 DAXWLG010000000
VERSION     DAXWLG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.148"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..460)
                     /locus_tag="ACGE4T_002258"
     CDS             complement(<1..460)
                     /locus_tag="ACGE4T_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8387476.1"
                     /translation="MRCQQHLTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQM
                     TTVISVFGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLY
                     GIGDCIFTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="ACGE4T_002259"
     CDS             complement(500..1552)
                     /locus_tag="ACGE4T_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8387477.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="ACGE4T_002260"
     CDS             complement(1565..2503)
                     /locus_tag="ACGE4T_002260"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8387478.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2954..3604)
                     /locus_tag="ACGE4T_002261"
     CDS             complement(2954..3604)
                     /locus_tag="ACGE4T_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387479.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3704..4213)
                     /locus_tag="ACGE4T_002262"
     CDS             complement(3704..4213)
                     /locus_tag="ACGE4T_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFF8387480.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4576..4959)
                     /locus_tag="ACGE4T_002263"
     CDS             complement(4576..4959)
                     /locus_tag="ACGE4T_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8387481.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            complement(5200..5814)
                     /locus_tag="ACGE4T_002264"
     CDS             complement(5200..5814)
                     /locus_tag="ACGE4T_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8387482.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         1829 a         1195 c          849 g         1949 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2881 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     2941 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     3001 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     3061 tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     3121 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta ttgagttgat
     3181 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     3241 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcacttca cctaatgaat
     3301 caattatttc ttgcccttta gctctaatat attgatctct attacctatc tcactcttat
     3361 ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc
     3421 tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac tctgatttat
     3481 tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca aaattgagcc
     3541 tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta tattcatcat
     3601 tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac aaaaactagc
     3661 tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact tttctccgat
     3721 tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa ggcttaaaat
     3781 ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga tagagttctg
     3841 cctcagcaaa aacttctaaa atatcatcag tggtcataga caaggctatt tttaattttt
     3901 ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg tctactatcg
     3961 gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat gcgtcaaaaa
     4021 cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg ttcttatcct
     4081 gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg cctagctcaa
     4141 aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc aataacctat
     4201 cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg tactttttgt
     4261 cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgatac ggagctgggt
     4321 atcatgagtg aatgatattt ataaatcact ggatattgaa gtatattctg ttttctcaac
     4381 ttatttagtc agatttttta atgaagattt tttgagtaaa atttctataa ttcctactta
     4441 ttgaaaagta gtttgtagtg ctgcttctac ctctctcatc tgttctacaa attccaaata
     4501 acttgacgca gcttgcggaa tgaaaaaatg atatgtgaat atttctcctc ctagcccgct
     4561 taatatgcct attttttata cccatgattt gcaggaaggg gatcggaagt aggttgcaga
     4621 aagccgcctt tcataatctg tcggaaaccg gacatcaaaa cggattattt tacttgtcac
     4681 atttgtattt tcttgtgagg aagtgccaaa tacattattt acttgagtga cattctcatt
     4741 gtcctgtctg atattgcttc gtataaaagt accagatcca cgtctcgtat ataacaaacc
     4801 ttcatcaata aggagttgta tagctttgcg gatagtaaca cggcttgttt taaactctct
     4861 agccagactt tcttgctcag gtaatttaga accttcctga tattttccct caacgatcct
     4921 ctcttttaga gccaaggcta tttctttata ttttggcata ttttcccctc atttcattga
     4981 tcctacaaaa aaattagtat atatacaaat ttttgtaaag tatatatact aataaataca
     5041 aaaatatttt tattgttgga tacagaaatc tatttttaac tggggatatc tttgccagct
     5101 aatatctttg agactacacc tatcttcatc taaatggatc aattactcgc tttttccatt
     5161 gaccttcgtg gtacttctgc tttctgatag taaaaaacat tactgaatga tcttactata
     5221 taacatacag cggagatcta cttctttatt aaggatcttc atgacgttat ctttttcttt
     5281 tttccgttcg aaaccgcgtc gttcataaaa ttgatacgta cagccgctat ccgtgaacag
     5341 aaatacttct tttccttgct ctcggcgttc aaattctttt aataatttac taccgatccc
     5401 cttgatcttt aaatcagggt tggcagctaa aaagacaatt tctccatctg gggaatgttt
     5461 ttttaaatag tctgaaagca attcgtcatt ggtgtcgtca tacccgcctt tgctgtcttt
     5521 cacaaatgca ttttgtaaga aatcaaatag tttaacatat aacttttgac taaatgaatg
     5581 atgctttttt gattttcctt tgatgtctgc taataaaaca cctgccaact catcatccgc
     5641 atataaagca atgatttgtg tcgcgcgatt caattctaga taccaaaagt atcttccata
     5701 aagattcaaa aggagtttgt tcttggtata ccaatcaaag tgcatacctt taatcgcgaa
     5761 ttgaatggct tttttgtagt ctttcttttc cagttgctta aaggctaatt ccatcactta
     5821 tt
//
LOCUS       DAXWLG010000147         5810 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXWLG010000147 DAXWLG010000000
VERSION     DAXWLG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.149"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            77..638
                     /locus_tag="ACGE4T_002265"
                     /pseudo
     CDS             77..638
                     /locus_tag="ACGE4T_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(749..1723)
                     /gene="lacD"
                     /locus_tag="ACGE4T_002266"
     CDS             complement(749..1723)
                     /gene="lacD"
                     /locus_tag="ACGE4T_002266"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8387483.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(1742..2197)
                     /locus_tag="ACGE4T_002267"
     CDS             complement(1742..2197)
                     /locus_tag="ACGE4T_002267"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFF8387484.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(2225..3670)
                     /locus_tag="ACGE4T_002268"
     CDS             complement(2225..3670)
                     /locus_tag="ACGE4T_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8387485.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(3667..4602)
                     /gene="pfkB"
                     /locus_tag="ACGE4T_002269"
     CDS             complement(3667..4602)
                     /gene="pfkB"
                     /locus_tag="ACGE4T_002269"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8387486.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            4770..5528
                     /locus_tag="ACGE4T_002270"
     CDS             4770..5528
                     /locus_tag="ACGE4T_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8387487.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
BASE COUNT         1824 a         1242 c          955 g         1789 t
ORIGIN      
        1 atctatgttc agatagcggt gaaattgtcg gataaccgac acaattcctt gattatgttg
       61 attaggaggc tgcaaaatga aaaaagagga tcgccgcgtc aaaaaaacag aaaaagcgtt
      121 agcagaagca ttatcaaaat tattagtaga taaaaaaatt caaaacatca ccatccgtga
      181 actgaccgaa actgctgatc ttcaccgttc gacattttat acacattata cggatatcta
      241 tgatctttat gaaaaactgg aaaatagctt tttccaagac ttggatgaaa tcatttccta
      301 cgatacgaca catagctacg aggatctata cgctcaattg atcgactatg tctatacgaa
      361 tacgttcgta tacaagttat tcgtcatcaa ccacggagat atccaatttc aaaagagatt
      421 aaataatctg atcgaagaaa attatttaaa aatctggcta caagaagaca aaaaaaccgt
      481 gatcacagaa gaaatgcgtt acttcaccat ctaccatatc caagggtgcc tctccgtttt
      541 aaatcattgg gtagaaaagc aattttgcat actctaagaa aaaaatgctg accttcttgc
      601 gcttattgaa cgacaacata gaacctatca tgccttaaaa acaaacgaaa aaggaagcgt
      661 cgggacgctt ccaattcttt atttaaaagc tcaaattgat tattaaaaat cattaggtta
      721 caaacttaag gttatagtta aagctttctt acaatttaat agctgttgct gtttctttca
      781 aaacattatt cagctcttcg atgttcttgc gtccttgtgt ctgcatccat tctttggcag
      841 catattcacc tttagtaacg aatggttcta caccattcgc ccaagttgcg cgcccacaga
      901 gtaccccatt gaatgtagag ccagcctctt ttgcaaattt caaggtttct tggaacaatt
      961 cagcagttac tcctgcgctc aagaaaataa atggcaggtt ggtagtctcg gattgttctt
     1021 tgaaataagc agcagcttct tctttggtat aagcaacttc atttttagtg taaccatcca
     1081 catagttcat attaacagga acttccactt tcaaaacgtc aactccatag cgaggcttag
     1141 aaaattcctt catcatttca tttactttat gcggtttttt caaggcatat tccttcgaag
     1201 aagtatcagg atcgttggca tcataagaaa ccaattctaa gaaaaatggc aaatcttcag
     1261 caacacattc agaaccaatc cgctccataa acgccttctt ttggtcattg atgtcatcag
     1321 attcatctac atcgtagtat aacaagaact tacaagcatc ggccccttgc tcctttagac
     1381 gttttacaga ccaaatagat aaaatatccg gtaaacgtcc tggagttgaa gcatcgtaac
     1441 cagttttttc ataggccaac aacagaccag catttttatc gcgaacttcg gctgccggca
     1501 acccatattc tggatctaat aagattgctg aagcatatgg agtcaattca gaagaaatga
     1561 tttttttgaa attgatgatt tgttcatcgg ttgctgattt attttgaaat ttaccgatca
     1621 tttttttcaa ggcaccacgt tgatcgattg ctaacgcact gatgatgcca ctttcattac
     1681 ttagttgacg aatataagtt ttcttttttt ctgttaaagg catgatttca ctacttcctt
     1741 tttagttttc ttctaatttt tggtttaaaa gttcagctat ttcttcagca gactgggctt
     1801 gtttcagacc ttctgtcaca tctttgtgca tcaaaagtct ggctacctga gataaaaatt
     1861 ttagatgggt tgttccagcc tctgaatctg gaataaacag agcgattaca aaatccgttc
     1921 cttctccatc taaacttttc caatcgacct taccgtttaa ccgaacaatc acaatatcgg
     1981 catccttgat actagcatct ttggcatgag ggatggcaaa accatccatc ataccagtcg
     2041 tgctttcttc ttctctttcc atcaattttt cgtaaactgc atctcggtct tcactaatgc
     2101 tcaagacaat tgactgatct gcaaggaagt tcattacctc atcttgcgtc gtaaaatttt
     2161 tattcataaa aactttattt gttttgatca aagtcatttg aaatccttct ttctagaata
     2221 acctttagtt gttggttgct tctttcattt caacagcagc tgcttctgct tcacgttttt
     2281 ctacactacg tttttggatc aaccctaaaa ttactccgcc aacgattgaa ccaagtaaaa
     2341 tcgaccctac ccatgcaacc ttaccagtaa caacaggtaa tactagaaaa cctccatgag
     2401 gtgcgggaac ttggacacca aacatatatg tcagtacagc agcaattgat gaacctagca
     2461 tcatagttgg cagaacacgc agcggatctt tagccgcaaa tggaattgct ccttcggtaa
     2521 tgtgcgttga gcccaagata aagttgacta aaccagcgtt gcgatcattc gtgtcgaaat
     2581 attttccaaa aaacagtgcg gcaaatccag taatcaatgg gggcgcgata caagctgccg
     2641 aaacccctgc cataaatgta tggttacctt gtgccaacaa cgcagtccct gtaacataag
     2701 ccgctttatt gactggacct cccatatcaa atgctgacat acagccaacg ataatcccta
     2761 aaattaatgg attagaattt tggaatcctg ccaagaaatt catcattcct tggttgactg
     2821 cttccattgg atttgctaat aaagacatga ctacaccaac aaacaagaca ccgatcaccg
     2881 gatataagaa gatcgccttc agaccatcca attgtttagg caagcctttc atgacctttt
     2941 gcaatagtaa gataaagtag cctgctaaga aacctgcaaa aattccgcct aagaaaccgg
     3001 ctccgccaga attggctaac agtccgccaa caaacccaac aaccaaacca ggacgtttag
     3061 cgattccttc agcgatataa gctgctaaaa taggaaccat cattcccatt gccgcatcac
     3121 caacaccctt taacatagcg gcagtcggat tgtaagtagg atcattggga tcaaaagagt
     3181 acacacccca gatggcaaac gaaattgcaa taattacccc acctgccact acaaacggca
     3241 gcatatgaga aacaccgttc atgagatttt tataaatact gtgaccaatg cctgctgcaa
     3301 cccccatctc cgctttctca ccagcagcgt caactgtttt gttttctgca gtgattgaac
     3361 ctttaccagc caacgcatct tcgatcaatt ggtcagcatc tttaatcccc ttgctgacag
     3421 gcacatcgat tacacgctta cctgcgaaac gttctgcttg aacatctttg tcagctgcaa
     3481 tgattacagc gtcagcgttt tttatttctt ctggtgttaa ttcattctct atccctactt
     3541 gaccatgggt ttcaactttg atagataccc ctttgcgctt agctgcctgc tccaatgctt
     3601 cttgagccat ataagtgtgg gcaattcctg ttggacatcc tgttgcggca acaattttgt
     3661 aagttgtcat tttttactcg ctccctcttt gatttcgatt tgtttttcca actctgcaac
     3721 atccgaaaaa tctgttaatc ctttacgaaa agcggttgaa cttccagctg ctaaactttt
     3781 cttcaaatta atatctacct gttcaccttt taaatatcct gcaagaaatg ttcccaacaa
     3841 agtgtcacca gcacacgctg tattgactac tttaccttta ggagaattcc cagaataaac
     3901 gatatcctta tttaaaaata ttcctccctc tcctcctaga gaaaggagaa catttttcgc
     3961 tccatcggca atcaagcgtt cagcataatg aatatatcct tcacgatcac taagctcgac
     4021 attataccaa gattgcaatt cctcctcgtt tggttttaat aagaacggct gatacggcag
     4081 acaatcaatt atttccggat agcttgaatc aataatcaga tggaccttat tttcaaaaca
     4141 aatacgactg atatcaataa gaatttcggg tgagacccct cgcggcaaac tgccagaaac
     4201 acacaaataa tcgtcagctt gcagcttttg tatttgattt agaaaagttt tgatttgttc
     4261 ctgaggaatc tcaggtcctt tattaaccaa tttgaattct tcattggttt tattaacttg
     4321 tgtaaaaacg ttgattctag tcattcccgg tacgctaata aaatcagtgc taatatcttt
     4381 ttgatgcaaa taatcctcaa tataattccc ggtaaaacct gctttaaatc ctaaagctgt
     4441 actatcgata cctaattgct tcatgatcaa tgaaacattc acaccttttc cattagcttg
     4501 tatatcatca tcgtttgttc gattgacttg ggatggtttc atttcatctg tttcgataaa
     4561 aaggtcgatt gccaagttta aagtacatgt ataaactgac atttacgctc ctcctttctt
     4621 acatattcat tatgtatcaa gatcgaatgt aaatgttttc ataaatgact aaattcattg
     4681 aaaactttta caaaatatat taaataaact tgaagtaaag gaaactcccg ctttacaata
     4741 gaattatcct aactaatcgg aggcaaaata tggatacaaa attaagtgaa tcggagaaat
     4801 atctctggga ttttattcaa agtcacatac tcgaaatccc ctcctattcc attgtaaaac
     4861 taagtgaata tgccaacgtt tcaacagcaa ccgtcgttcg tactatgaag aaaaaaggat
     4921 atgaggggtt tacgtctttt aaacattcgt taaaagaaaa agaaaataca aatatcaatt
     4981 ttgccgctct tgataaagtt gatgaaggaa taaagactgc tattcttaaa aatgagcaag
     5041 aggtaatccg caccatcaat atggtagaaa gcgggaatat tgaagatgct attcaacgaa
     5101 tcaaggctgc tagaagaatc atgatttttg cccgaggttt ttcagaattg attgctcagg
     5161 aaatgatggt caaatttcaa cttaccggta aatattgtga gatgcatact gatcccaata
     5221 tcataaaaaa cattagtaga aaattatcca aagatgacat cgtgatcttt gtttcactaa
     5281 acggtgaaac aaaggaattg actaccgcag ctgaaaactg ttataaagct gagatcggga
     5341 caattttgct tactgctaat cgtcattcaa gtttgatgga acatatcgag attccttttg
     5401 ttggctttaa atcagaaggc tccttcttcc cagattatga ggtacgctcc agacttccac
     5461 tttctatact ggctagaatc ttacttgact cctatgcact gcgaacatca ggattcacac
     5521 agcactaact ctatatctgt aagactgtac ctaaaatata tctgtgtgaa ttaactaagg
     5581 ttgtctagaa aaatatttaa tcgctatttg tattctcgtg ctttctatca ttgctccttc
     5641 tataacttct tagtttcaag atagaataat gcattgagac taccaggact acttacgcaa
     5701 atatataata ataagaatgt gataagcagt tcgaagttcc tgtctaatat ttcttgttaa
     5761 ttattatcaa tttccaacaa ttctagaatt ataatacgca aaaaaaggag
//
LOCUS       DAXWLG010000148         5767 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXWLG010000148 DAXWLG010000000
VERSION     DAXWLG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.150"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            92..988
                     /locus_tag="ACGE4T_002271"
     CDS             92..988
                     /locus_tag="ACGE4T_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFF8387488.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1138..2079)
                     /locus_tag="ACGE4T_002272"
     CDS             complement(1138..2079)
                     /locus_tag="ACGE4T_002272"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8387489.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2205..3326
                     /locus_tag="ACGE4T_002273"
     CDS             2205..3326
                     /locus_tag="ACGE4T_002273"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8387490.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3584..5086
                     /gene="eat(A)"
                     /locus_tag="ACGE4T_002274"
     CDS             3584..5086
                     /gene="eat(A)"
                     /locus_tag="ACGE4T_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8387491.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5153..5680
                     /locus_tag="ACGE4T_002275"
     CDS             5153..5680
                     /locus_tag="ACGE4T_002275"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8387492.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1883 a         1023 c         1219 g         1642 t
ORIGIN      
        1 ttcatacaaa tataggaaac gttttactgg ctttttacta tttaaacagg ctatcatagg
       61 aaaaagtgac agagaaagtg aggcggaaaa gatgaacaaa aatgacgtac tgctagttgc
      121 aaaaaataaa tcactttatt ttttgccttc catccgctct ttagatgttt ggtccggaaa
      181 agatttacta cagtcggaat tcattaaatc agctagacaa cagttggcaa attatcagct
      241 gatcgttttt ttagattatg gatttgatat ggagatggct cgacgcgtgc gcccatttac
      301 caatgcaaaa attgtgttat ttttttggaa tcacttcaaa gcagaacacg aggcgatgct
      361 gaagcaggca caagatgaac cggcaatcga tgagatttat cattttgatt ttttggaagc
      421 aaaagaactt gggctgaaac ataatagttc attttattcg aaacatatga atttttcttc
      481 tagcgaacca aagacggatc tattttttgg tgcaacgaat aatgggagaa aagaacgagc
      541 agaaagctat aagcaggaat ttataaaaag agatgttacg accaactatt tcattcttcc
      601 acatcgaggg aatgaacaac ctggatatct tacttatgaa gagtacctga aaaaaacagg
      661 ggaatcgcgc gggattctgg aactgttgcg ggagggtcag caaggagtga ctttgcggac
      721 atttgaatcc atgtattttc aaaagaaact agtgacggat aatcaagcga ttgctcatta
      781 tcatttttat cgtcctgaaa atattttttt gcttcgagaa cgtgatttag atgaattacc
      841 ggaatttctg catacaccat ttaagccaat cctatcagag ctattggaat tttatgatgc
      901 agaaaattgg gcaaaacgat ttatcactaa cgattccact atttatgagc aatacgaata
      961 tgacctctcc tctgttgaaa acaaataaaa aacctacatt ttgaagagat tatgtactga
     1021 aacaaacaag cacaattctt atggcatcat gagagttgtg ttttcttttt gatcttttat
     1081 tgggcttaaa aaaagagaac cagctgctga ttcagctgat tctcatcaga gagcctttta
     1141 ttctgcgcca aggattcctt cttttgcatg gactaattgg gtcaaaaagg cacaatatgg
     1201 tgtagcaata tcatattttt gtccttttcg ccaaatggca ccattgatgt agtcgatttc
     1261 agtcaaacga tggtttttga tcaggtcttg gtacatagat gggtaatgca acccgatgtt
     1321 gtcaggatca taagtagaag caatatgttg gtacacttct tcttgatcta gtatgattcc
     1381 ttcttttgct gcgatagaag caaattcaga tacgattgtt cgaaccatgc tctctgccgc
     1441 tttttgcgcg cctagttcag caatattaca atccaaaatc gtgcataaac cattcaatgt
     1501 tccatttaca caagcttttc gccaaatgga ataacgaacg ttatggctat agatcggatt
     1561 taaatttgct tcttgaaaga cttctacaac tttttttgta aatgcttcgc cttctggttc
     1621 caagttctct agttcgattt ctccatcacc taacaatgtg acttttccgg gaccagctag
     1681 accagctgtc cacatcgtga tcccaaataa gatatttttc tttggcacat atttttccag
     1741 tacatcttca tgacctaatc catttagtaa acacaaaaca taagtattgt cagtaataat
     1801 ggattggatc gaacaaaaca tatcatctaa ttgattggct tttgttaatg ctacgatcaa
     1861 gtccacgtgt tcgttgcttt cgatgatttc ttctggtgaa tagattggca gttttgcgac
     1921 cacttcttta ccgttaaagt cagcaatcaa cccatttgta cgaatggctt caatgtgagc
     1981 aggccaacga tcgatcaaga ggacctcatt tccgctttga tgtagcatca atccgattcg
     2041 gctgcccatt gctccggccc cagctattgc gattttcatg ttttttctct cctttattca
     2101 atgagttccc ttttagtata tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa
     2161 aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat acagatggat attttggcgg
     2221 caattgattc atttaaagga agtgcttctt catttgaact aaatcaggca gttctaaatg
     2281 gctgggaaga tcgtaaagga agaaaaatca atgtgccgat tgcagatggc ggagaaaata
     2341 ctgcagaagc aatctattac gcgctcaggg gaacatggcg taccattgat gggatggatc
     2401 tcttattccg tcagcgaaaa atcagctatt tattgacaac ttggcaagga aaaaacaccg
     2461 cagtcatcga atctgcacaa gcattggggt tagatctact tgataaacca acggatcaaa
     2521 ctatccgact tgcttccagt tatgccttag gagaacttat taaagatgca ctgcttcaag
     2581 aagtagaaca aattattgtc actttaggag gaagcgggtg tagcgatggt ggtctaggat
     2641 tactccaatc cttgggtgct tctttagaag ggtacacaga aggaaatcct ttattatcta
     2701 ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg
     2761 ctgcagatgt taccaatcca tatacaggtc aacaaggagc tgcttggcaa tttggaagac
     2821 aaaaaggagg aatagatacg acgctagctt tcttagataa acaagcagga aagattgctc
     2881 agcaaataaa agatttctgg gaggtggatt tagatgatat tccaggaagt ggagcagcag
     2941 gcggtattgg tggagccctt tttttgttgg gagcgaagat ggtttctggt ttcgatttag
     3001 tcagccaaat cactggattg gaaaaaaaga tcgaacaagc agatgtcatc attacaggag
     3061 aagggcggat cgatcagcaa acgatccacg gaaaagttcc atacggtgta gcagtgcttg
     3121 caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccaa gagctagaag
     3181 gactttctga ttttctccca attgtcttaa gtattcattt gggtccaata ggtttagaag
     3241 aagcaatgga acacaggcaa acattagaaa aaacaaaaat cttaggacag acacttagca
     3301 gatttttcac catcctcccg aagtagttga caaaatcatt ttctttatgc tatcgtaatt
     3361 tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc
     3421 tgcattcaga ctgtaccttg tggagacaag gaagcagtgt gcagattttc gggattggct
     3481 tggttttttc gatgtatggg tctttttgtg tgtatccaac ccttgcatcc tctttagcca
     3541 gttctgaaaa taggactggt ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat
     3601 aaaaaatctg acattcggct acgacagcca aggcacatta ttatttgaac aagcaaatct
     3661 aaattttgac acacaatgga aactaggact tatcggacga aacggtcgag gaaagacaac
     3721 tttactgaat attctacaaa acaaactacc ttatcaaggg caagtaatcc atcagcaaga
     3781 atttgcctat ttcccgcaac agacaaaaga taaagaacgt ttaacctatt acgtgttaaa
     3841 tgatattacg gattttgaga tatgggaaat cgaaagagag ctccaattga tgcaaacaga
     3901 tcctgaaatc ttatggagag aattcagcac actatcgggg ggagagaaga caaaagtcct
     3961 actggcactt ttatttgtgg atgacactca tttcccgtta atcgatgaac caacgaatca
     4021 tttggatatc tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt
     4081 catcgtggtc agccatgacc ggggatttat cgatgaagta gtggaccatg ttttagcaat
     4141 cgaaaaaagt caactggaac tttatcaagg gaatttctct atctatgaag aacagaaaaa
     4201 acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct
     4261 aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa
     4321 gaaacaagtc ggattcatcg atactgaatc tagacgagtg aataaaggag cagtgggtgc
     4381 tgatgctgca cggacgatga aacgatccaa agcaatcgtg aatcggatgg agacccagat
     4441 cagcgagaaa gaaaaactat taaaagatat cgaatatatc gattctttga cgatgaatag
     4501 ccaagcgtct caccataagc gacttttaag cgtagaagat cttcaattag ggtatgaaaa
     4561 tctgttattc gagccaattc attttacaat cgagcctcat cagcgggtgg cgatttcagg
     4621 tcctaacggt gcaggaaagt catccattat ccattatctt ctgggggcat tcaacggcaa
     4681 ggttatagga gaaaaatacc agccaaaaca tctgagcatt agttatgcaa gccaaaatta
     4741 tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag actaccaagc
     4801 atttttgaac aacctccgaa agcttgggat ggaaagagat gtttttcata acaagatcga
     4861 gcagatgagt atgggccaac ggaaaaaagt ggaattggct aaatctttat cacagccagc
     4921 tgaactatat acatgggatg aaccattgaa ttatttggat gtcttcaatc aagaacaatt
     4981 agaacaactg atcttgaacg tgaaacctgc catgttacta gtggaacatg atcaaacctt
     5041 cctggataaa gtatctactg agattatttc tcttgagaga atctaaccct tgtaatctca
     5101 agaacaacaa ggtatggtta aggggaactt tgccaacgaa ggagagaatc acatgcttta
     5161 tgtaacgaga cacggagaaa caacttggaa cgcacaagga ttagtttgcg gacgggcaga
     5221 tgtgccgctg actgaaaaag gacagatgca ggcacagaaa ctagcagaaa aggtagtaga
     5281 tttgcctgtt ccaatcacga aaatcatcca ttcccctctt caacgagcaa gagatacagc
     5341 acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat
     5401 ggattttggg gattatgatg gactgccaag caaagatgag aactttcaaa aagcacgttt
     5461 agcgttcgct gtacgattcc caaatgggga gtcagtctta gatgtgtatg cacggatcgt
     5521 tcctttattg aaagaatgca tcgaagacga agaaaatgtc tatctgttag tttgccataa
     5581 tgccttgata agagtgatca atgcttattt tcatccgatg cctaatgagg ggttctttac
     5641 attcatggta gataatacag aattggtttc ttatgagtaa ttatgttttt gtgttattta
     5701 aataaaatat ttcaaggcac tgcttttgct tagcagtgcc ttttttaggt tatgacaatt
     5761 cttttga
//
LOCUS       DAXWLG010000149         5744 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXWLG010000149 DAXWLG010000000
VERSION     DAXWLG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.151"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(233..1015)
                     /locus_tag="ACGE4T_002276"
                     /pseudo
     CDS             complement(233..1015)
                     /locus_tag="ACGE4T_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1080..1916)
                     /locus_tag="ACGE4T_002277"
     CDS             complement(1080..1916)
                     /locus_tag="ACGE4T_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8387493.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1934..3343)
                     /locus_tag="ACGE4T_002278"
     CDS             complement(1934..3343)
                     /locus_tag="ACGE4T_002278"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8387494.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3357..5180)
                     /locus_tag="ACGE4T_002279"
     CDS             complement(3357..5180)
                     /locus_tag="ACGE4T_002279"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8387495.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5460..5687)
                     /locus_tag="ACGE4T_002280"
     CDS             complement(5460..5687)
                     /locus_tag="ACGE4T_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387496.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1758 a         1253 c          941 g         1792 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatcttttta cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
      481 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
      541 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
      601 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
      661 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
      721 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
      781 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
      841 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
      901 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
      961 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
     1021 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
     1081 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
     1141 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
     1201 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
     1261 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
     1321 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
     1381 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
     1441 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
     1501 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
     1561 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
     1621 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
     1681 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
     1741 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
     1801 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
     1861 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
     1921 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
     1981 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
     2041 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
     2101 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
     2161 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
     2221 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
     2281 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
     2341 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
     2401 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
     2461 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
     2521 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
     2581 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
     2641 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
     2701 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
     2761 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
     2821 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
     2881 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
     2941 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
     3001 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
     3061 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
     3121 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
     3181 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
     3241 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
     3301 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
     3361 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
     3421 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
     3481 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
     3541 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
     3601 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
     3661 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
     3721 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
     3781 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
     3841 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
     3901 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
     3961 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
     4021 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
     4081 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
     4141 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
     4201 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
     4261 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
     4321 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
     4381 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
     4441 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
     4501 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
     4561 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
     4621 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
     4681 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
     4741 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
     4801 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
     4861 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
     4921 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
     4981 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
     5041 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
     5101 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
     5161 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
     5221 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
     5281 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
     5341 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
     5401 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
     5461 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
     5521 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
     5581 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
     5641 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
     5701 ggtatcaata gaatgatttg ggctagtatt cttaaaaatt aact
//
LOCUS       DAXWLG010000150         5743 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXWLG010000150 DAXWLG010000000
VERSION     DAXWLG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.152"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            198..3773
                     /locus_tag="ACGE4T_002281"
     CDS             198..3773
                     /locus_tag="ACGE4T_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8387497.1"
                     /translation="MISNKKRWLKVALLVAFCQIAFLASMTTQAEEIVQETTVSQVSP
                     TSNEVEVQSTLPKEAVKEETPSEVVPETEAPSTNEKTVLDETIGKSQQPGDSPVVAEE
                     TPVEAHEAQAIPEKDKSLEESGSTEINEGTEPVQLEDSKEVTTPIESTKEEMDSNTSL
                     SSEKEEKTVLLTEPREPELKKVEEFVDTKKEAVSPTSSKEDTLIVQQTARSARVRRAT
                     NTAVLGGRKIDWGENIGVISSANNPNFTLNYMVRFEINGQVAFCIEPGVAAAEGQVYT
                     ATALENYLKDLNIRKKTSLISYLGYVTSKDKSDEQYIAAQFMIWEVLGTKIPIKNIRI
                     DYDRRKAEIQRLLNNFDVRATFHNQTHTVKIGETLKVKSGNDFLQRVKDIQKPKGVNV
                     SIHDNEVWITITKESPEKAKIHFNQIEALGVPLAYRRPGAQTIGVLNPFVPGDSVVNL
                     TILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQANGKKIKEITLKNINGKVQAEI
                     KDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNTIAKDQVIKGTIDLVKVANKD
                     LVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTVVFDTYILSETK
                     GKEGYKLFEPFEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKVIALAGTQFKIW
                     DKYANNGKGGYVSMALPNDTEVSDIFTTNEKGYFVTTDTLKYGKNRYELREIKAPEKY
                     VLNKTPYVFSVTDDTNTIQLIYFANRLAKANVEVFKYDTSTNFDQKTAIAGVEFDLFK
                     KDETGTTFVGTFKTDKKGKINVKDLLVGDYFFKEVKPLAGYLALDKDVPFTVTVDKDG
                     TVIALEVENKRIPPKIGTTAIGKYTNQQKVTPLKKVTITDTVKYSNLIIGKTYTVNGV
                     LMDKEESITAGKDVPLLVNGKQITASKVFVAKNPNGMISLDFVFDASALAGKEIVVFE
                     NVYRDGRLVASHTDIHDKGQTVRIVNPMIHTKATGQNGEKQLQALPNQKVKELVQLND
                     LIVGQTYKVLVQGYITPDGVKLDGTSAEKIFKADKTTLEFLFEFLVDGRKLAGKGLSF
                     AEWLEAQSEPKDENDYEVRFEQIANHNVDLKSEDQTVYFVEVPKVPEPKIPEKSQPFK
                     EVKQASVVPMLPKTGSEDTVLWMIIGVLFVLAAGLTFYELRKVKD"
     gene            3873..4811
                     /locus_tag="ACGE4T_002282"
     CDS             3873..4811
                     /locus_tag="ACGE4T_002282"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8387498.1"
                     /translation="MKKKERKKRMKKLFFACLFSSVVLAGGINVFADEIGITPVTPPV
                     IEETTPVVPIPDISKIDESVPDQPTPVDPDISSEVTEPTTPVVPDITENSENETPVVS
                     PSTGETTNDKDTTPTTSESYSGTSSVDNDSKIDDFSSSSTTIDTPKETEKKNDLGAPK
                     NLTPENHIKTVSEKEMTVSVSSDGKIVQGTGTEKSIPIVTSNLEEINHVPTSETPLQV
                     ATGQAIVGVQDGVPLVQDNQGNLVEDLSIPVKKLPSGNIEIKTIDGKTKVLPKTGEEL
                     QISLTILGSLLTAVTGGIFYGKKQWRHLRHMFKKTI"
     gene            4830..5180
                     /locus_tag="ACGE4T_002283"
     CDS             4830..5180
                     /locus_tag="ACGE4T_002283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387499.1"
                     /translation="MGLSYVAISQEEVLGKCFNQKHVGSTGERDRRGNMKKVEWEIFQ
                     MNSEKMGEIEVRVAVGGAPKAMRYNAEVLLANPRISRRADVRNIGNDGETNKIARNRF
                     YLLVDRVEVKGGNN"
     gene            5180..5611
                     /locus_tag="ACGE4T_002284"
     CDS             5180..5611
                     /locus_tag="ACGE4T_002284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HFF8387500.1"
                     /translation="MTLEFDQGIVPGLVKETLGEIYFVKAQRPRMLYEDNENTGEIKE
                     RPVEFSSATQETMFVVNFPPKTNLDGLSFGDVVELEEVEFRFWISIDRESMSNRAEND
                     LKVSALRYKKTGKNVFQTSSSTRATYVKEKQDSNKESAKKE"
BASE COUNT         2143 a          899 c         1150 g         1551 t
ORIGIN      
        1 tttgaattaa attttagaaa caaataattt atcagcatat aaatactgat aaaagtttta
       61 tagacaaaga tgttcatagc gaacatcttt tttatttatt ttgagaagaa acagagcatg
      121 aaatgatgtc ttatttccat ccatatagtc tatttcaaac agaagagaac aattaaaaag
      181 aaaggagatt tcttgttatg ataagtaaca aaaaacgttg gttaaaagtt gctttactgg
      241 tagctttttg tcaaattgct ttcttggctt caatgacaac acaagcggaa gaaatagttc
      301 aagaaacaac tgtttctcaa gtttctccta cttcaaatga agtagaagtg caatctactt
      361 taccaaaaga ggccgttaaa gaagagacac catcagaagt tgttccagag accgaagcac
      421 cttcaacgaa cgaaaaaact gtactcgatg aaactatcgg gaaaagccaa caaccaggag
      481 atagtccagt tgtggcagaa gaaaccccag tagaagcaca tgaagctcaa gcaattcctg
      541 aaaaggacaa atcattagaa gaaagtggtt ctactgaaat aaacgaagga acagaaccgg
      601 ttcaattaga agattcaaag gaggtaacga ctccgattga atcaacgaaa gaagaaatgg
      661 attctaatac ttctctttct tctgagaaag aagagaaaac agttcttttg acagaacctc
      721 gtgaacctga attgaaaaaa gtcgaggaat ttgttgatac aaaaaaagaa gctgtttctc
      781 ctacctcttc aaaagaagat acattgattg tacaacaaac cgctcgaagt gcacgggtgc
      841 gacgtgcaac caatacagca gttttgggtg gtcggaaaat tgattggggc gaaaatattg
      901 gtgttatttc tagtgccaat aatcccaatt tcactttaaa ttacatggtt cgttttgaga
      961 ttaatggaca agtagcattt tgtattgaac ctggtgtcgc agccgcagaa ggtcaagttt
     1021 atacagctac tgctttagaa aactatttga aagatttaaa tatacgaaaa aaaacaagct
     1081 tgatctctta tttaggatat gtcacaagca aagacaaatc cgatgaacaa tacattgcgg
     1141 cacaatttat gatttgggaa gttttgggca caaaaattcc aattaaaaat atccgtattg
     1201 actatgatcg tcgaaaagca gaaattcaac gtttgcttaa taattttgat gtaagagcta
     1261 cttttcataa tcaaacacat acggtaaaaa ttggcgaaac gttaaaagta aaaagtggca
     1321 atgactttct acaacgtgta aaagacattc aaaagccaaa aggtgttaat gtatctattc
     1381 atgataatga agtatggatt acaatcacaa aagagtcacc agaaaaggca aaaattcatt
     1441 tcaatcaaat tgaagcatta ggcgtgccat tagcgtatcg tcgtccaggt gctcaaacaa
     1501 ttggggtgct gaatcctttt gtacctggtg actctgttgt caatttgacc attttgaaac
     1561 aaggaaacat tcgtgtcttt aaagaagatg cagaaaccgg tgtgaaagca caaggtgcag
     1621 cttctttaga aaatgcagtg tatggcttgt atcaagcaaa tgggaagaaa atcaaagaaa
     1681 ttacattgaa aaatatcaat ggcaaagtac aggcagaaat caaagatctt gatcctggta
     1741 aatatgtttt aaaagagatc aaagcaccaa aaggttatgt actttctgat gaagcaattg
     1801 aaatcgtcgt tgaacctgga aaaactgtca atacgatcgc aaaagatcaa gtgatcaaag
     1861 gaaccattga tttagtcaaa gtcgcaaata aagatttagt cgattcaacc aatccagaca
     1921 acaaacccaa attagctggt attgaaatct ctttgacgtc aaaaacaact ggaaaagtgg
     1981 tgaaaacagt tgtgacagat aatgatggtt acgctacttt tggtaaaaat accgttgtct
     2041 ttgatacgta cattctttct gaaaccaaag gaaaagaagg atataaacta tttgaaccat
     2101 tcgaagtcac aatttctgaa aatggacaaa ccttccatta tgttttagaa gacaaagtaa
     2161 tcgaacaacg tcttaaagtg gtgaaagtgg atcaagaaac agggaaagtc attgctctag
     2221 ctggtaccca attcaaaatt tgggataaat acgcaaacaa tggtaaaggt ggctatgtat
     2281 cgatggcatt accgaatgat acagaggttt cagacatatt tacaacaaac gagaaagggt
     2341 actttgtcac aacggataca ttgaaatacg gaaaaaatcg ttacgagttg cgagaaatca
     2401 aagcacctga aaaatatgtt ctaaataaaa cgccttatgt cttttcagta acagacgaca
     2461 ccaatacgat tcaattaatc tacttcgcta accgtttagc gaaagccaac gtagaggttt
     2521 tcaaatatga tactagtacg aattttgatc aaaaaacggc gattgctggt gttgaatttg
     2581 acctattcaa aaaagatgaa acaggcacaa cctttgttgg tacattcaaa acagacaaaa
     2641 aaggtaaaat caatgtgaaa gacttattgg ttggcgatta cttctttaaa gaagtgaaac
     2701 cattagcagg gtatctggcc ttagataaag acgtaccatt tactgtaacg gttgataaag
     2761 acggtacagt gattgcacta gaagtggaaa acaaacggat tccacctaaa attggtacta
     2821 cagctatcgg taagtataca aaccaacaaa aagtgacccc attgaaaaaa gtaacgatta
     2881 cagacactgt aaaatacagt aatctaatca tcggaaaaac ttataccgtc aatggtgttt
     2941 tgatggataa ggaagaatct atcacagcag gaaaagacgt accgttgtta gtaaatggta
     3001 aacaaattac cgcaagtaaa gtgtttgtcg caaaaaatcc taacggaatg atttcccttg
     3061 actttgtctt tgatgctagt gcattagctg gaaaagagat agtcgtattt gaaaatgtct
     3121 atcgtgacgg acgcttagtt gcgtctcata cagatattca cgataaagga caaacggttc
     3181 gtattgtgaa ccctatgatt catacaaaag caacaggtca aaacggcgaa aaacaattac
     3241 aagctttacc aaatcaaaaa gtgaaagaat tggtacagtt gaacgactta attgttggtc
     3301 aaacgtacaa agtgctcgta caaggttata ttacacctga tggtgtgaaa ttagacggta
     3361 caagtgctga aaaaattttc aaagcagata agacaacttt agagttctta tttgaatttt
     3421 tggtagatgg acgtaagcta gcaggcaaag gattatcttt tgcagaatgg ttagaagccc
     3481 aaagtgaacc aaaagatgaa aacgattatg aagttcgatt tgaacaaatc gcaaaccaca
     3541 acgtagactt aaaatcagaa gatcaaacgg tttattttgt agaagttcca aaagtaccgg
     3601 aaccaaaaat tcctgaaaaa tcacaacctt ttaaagaggt gaaacaagct tcagtagtac
     3661 caatgttgcc aaaaacagga tcagaagata ctgtattatg gatgatcatt ggtgtgctat
     3721 ttgttttagc agcaggtctt acattttacg agttacgcaa agtcaaagac taagtaggac
     3781 aaacaagcgg ttagatgcct aatcgcttgt ttggttttaa aaaagttatt gcataagtaa
     3841 cctttgaaaa gataagaaat gatctttgtt ttatgaagaa aaaagaaagg aaaaagagaa
     3901 tgaagaagct atttttcgca tgtttattta gttccgtagt tcttgctggt ggaataaacg
     3961 tttttgcaga tgaaataggt attacaccag ttacgccacc tgtgatagaa gaaacaacac
     4021 ctgttgttcc aattccagat atttctaaaa tagatgagtc tgttccggat caaccaactc
     4081 ctgttgatcc ggatatatct tcagaagtaa ctgaaccaac aacacctgtt gttcctgata
     4141 taacggaaaa ttcagaaaat gaaacaccag ttgtatcgcc ttctactgga gaaacaacga
     4201 atgataaaga tactacacca actaccagtg agtcttattc gggaactagt tcggtagata
     4261 atgattcaaa aatagacgat tttagttctt catctactac aatagataca ccaaaagaaa
     4321 cagaaaagaa aaatgattta ggagctccca aaaatttaac acctgaaaat catataaaga
     4381 ctgtatcgga aaaggaaatg actgtttcag tcagttcaga tggaaaaatt gtacaaggaa
     4441 cgggtaccga aaaatcaatt cctattgtga caagtaattt agaagaaatt aatcatgtcc
     4501 caacatctga aactccttta caagtagcga ctggtcaagc gattgttggg gtacaagatg
     4561 gtgtaccact tgtacaagat aatcaaggga atttggtaga agatttatcc attcctgtaa
     4621 aaaaactacc tagcggcaat attgaaatca aaacaatcga tgggaaaaca aaagtattgc
     4681 caaaaacagg tgaagaactt caaattagtt taactatcct tggttctctt ttaacagcag
     4741 taaccggggg cattttttat ggtaaaaaac aatggcgtca tttgcgtcat atgtttaaga
     4801 aaactattta gtaaggagag agaataaaaa tgggtttatc ttatgttgca attagtcagg
     4861 aagaagtatt gggaaagtgt tttaatcaaa aacacgttgg ctcgacaggt gaacgagatc
     4921 gtcgaggaaa tatgaagaaa gtagagtggg agatttttca gatgaactct gaaaagatgg
     4981 gagaaattga agtgcgtgta gctgtcggtg gtgcgcctaa agccatgcga tacaatgcag
     5041 aagtgttact ggcaaatcct agaatttctc gtcgagcaga tgtacgaaat attggaaatg
     5101 acggtgaaac aaacaaaata gcacgtaata ggttttattt gttagtcgat cgtgtagaag
     5161 taaaaggagg aaataactaa tgacactaga atttgatcaa ggaattgtcc cagggttggt
     5221 aaaagaaaca ctaggagaaa tctattttgt caaggcacaa cgtccacgta tgctgtatga
     5281 agataatgaa aacacaggag aaattaaaga gcgtccagtt gaattttcaa gtgctaccca
     5341 agaaacaatg ttcgtggtga attttccacc aaaaacaaat ttggatggat taagttttgg
     5401 cgatgtggtg gaattagaag aggttgaatt tcgattttgg atttctattg atcgcgaatc
     5461 aatgagtaac cgagcagaga atgatctgaa agtatctgcg cttcgttata aaaaaacggg
     5521 aaaaaacgtt tttcaaacgt cttcctctac tcgtgcaaca tatgtaaaag aaaaacaaga
     5581 ctcaaataaa gaaagtgcaa agaaagagta atggtttcca ttgctctttc ttattttaaa
     5641 gctagaagaa gtagctgatg tggagaaaat tgaattggct ggctatatta ttgggataaa
     5701 tttttgaatt tttaagaatt tagatagtgg ttagatacga ttg
//
LOCUS       DAXWLG010000151         5723 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXWLG010000151 DAXWLG010000000
VERSION     DAXWLG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.153"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..268)
                     /locus_tag="ACGE4T_002285"
     CDS             complement(<1..268)
                     /locus_tag="ACGE4T_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFF8387501.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVID"
     gene            complement(523..843)
                     /locus_tag="ACGE4T_002286"
     CDS             complement(523..843)
                     /locus_tag="ACGE4T_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387502.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(876..1964)
                     /locus_tag="ACGE4T_002287"
     CDS             complement(876..1964)
                     /locus_tag="ACGE4T_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387503.1"
                     /translation="MQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYRQNKQTGIHFL
                     GYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSRIMRNYPLPSN
                     TYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYANPKSQSMFAD
                     YGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKT
                     DPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAGVFRDRMVRYE
                     QKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKG
                     FENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(2023..2817)
                     /locus_tag="ACGE4T_002288"
     CDS             complement(2023..2817)
                     /locus_tag="ACGE4T_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387504.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(2827..3702)
                     /locus_tag="ACGE4T_002289"
     CDS             complement(2827..3702)
                     /locus_tag="ACGE4T_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387505.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(3919..4176)
                     /locus_tag="ACGE4T_002290"
     CDS             complement(3919..4176)
                     /locus_tag="ACGE4T_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387506.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(4179..4403)
                     /locus_tag="ACGE4T_002291"
     CDS             complement(4179..4403)
                     /locus_tag="ACGE4T_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387507.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(4576..5619)
                     /locus_tag="ACGE4T_002292"
     CDS             complement(4576..5619)
                     /locus_tag="ACGE4T_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8387508.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         1580 a         1214 c          737 g         2192 t
ORIGIN      
        1 catcaataac tgtagcaata tattgagaga tagaaatatt atcttcactt ctatgtcgct
       61 ctataagatt atatatatca gggctgacat gaaacgtact tctctttttt gtttgccttt
      121 ttctaactgt tacgaccact tttggaactt cactatctcc attgatctct ttgtattttt
      181 caataaataa ttttctagct tgttctcgac tttttgccgg aatttcatct acaattttat
      241 cttctaaata aatatagtgt gttctcatac attcgtcctc tatcttcttt actctcaatc
      301 ttcatgatct tagttcttac cttgtttact cttaaacatt gcaaaaaaat ctagtcaaaa
      361 tcaaacttga aatcatcgtc tatcacaaca cccttaactt catctttaga tttcccaaag
      421 gaacccttat tttcaaatcc tttatttttg aatgatgatg gttatggaaa acttcctttg
      481 aaattctgac aagcataact agtcagaact gttttactta gattaagctt tcaaaagata
      541 ctgatgaaga actaagttct cagtaccttc ccattcttca ataaaaaata tttctccttc
      601 ttttacccac tctatagcta atttcttata atcaaataaa ccatatgtgt ttaagccttt
      661 gtctttcatt aaactcaaaa actgatcccc ttttaatctt tctgtatcat ccatcagaaa
      721 ttcaactaaa tctttatcca tgcgtgcaac tttttcgtct tttagtgcca ttgatttagc
      781 atagtcagtt gaatctttta gaataataac acctacattt ccttccacaa ttttcttctt
      841 catatttacc tcctaccatc ttttccaaag agtatttagt caaaatcaaa ctcgaaatca
      901 tcgtctatca caacaccctt aacttcatct ttagatttcc caaaggagcc cttattttca
      961 aatcctttgt ttttaaattc taaagctttt tcgacttctt ctttggatat tgtatgaact
     1021 tttttcttaa ctactccaag tttatacagc tcttcaaact ctggtacttc ggatctgaaa
     1081 tacaatccat cagtacgaat ttttaatagc ccatatgcgc tatgaacgta tttctgttca
     1141 tatctaacca tacgatctct aaatacgcct gctaccatgg aggaatacgc tcgattatac
     1201 atactgtatt tatcgaaggt gcttgtttct ccttctctta atgatttttg tccgatagca
     1261 ccaaatactt tcgttctaag atttttataa agcttcaacg tatcaggatc agtttttaat
     1321 ggctcgattc tttcaaaata ttctttgagt aatccttttt tcgtcaaaaa tgtgtggcta
     1381 tctaggactt ccatctcttt aatatcataa taatcaagga taagtgttag ctcaatctcc
     1441 gataaccaaa taggctcatt agctgttttg ggattttcgc cataatccgc aaacatcgac
     1501 tgtgattttg gattagcata tgttccttct ttggctgttc cttcaaaatt aaccaaaaag
     1561 aacatatacg tttttaaatt ttcatacggt cttacttttg gtctatttat cgctctcaaa
     1621 atagcttgat ctgtattata ttttgctttc aaataagtgt ttgacggcaa aggataattt
     1681 ctcatgatcc tcgaataggc ttcgttgaaa tcatattcga tcatctttgc tccttcctca
     1741 aacaaatcca agttatacat gctaccggag gtactggctc tataggctga acttggaaca
     1801 aagaaaggtg catcttccgg cttatcatag cccaaaaaat gtattcctgt ttgcttgttc
     1861 tgacggtaaa tgtccatcaa aagatatttg atcgtactaa aagaatctaa ttttcctctt
     1921 gtcatccacc ctaatccgac aagtctttta attttattct gcatttcagg tgtgtatatg
     1981 ctattgtttc ccatttatct gctcatctcc ggctttttta ttctagaccc tatatttgtt
     2041 cattacacca cttatctcgt ctacaaaggc tcccagatcg tgtgtaaagg catcatagta
     2101 atcttcaatc tcactcgatt cagttctccg gtttcttccg tttttatttc tgaatagata
     2161 catctgcgtt tctcttaaat cccttgcttt tttcgcaagt gctaataaat ccgtaaaatt
     2221 atcaataacc ataatgtcat tgatcataaa accttcttca tgaccatact tttcagccat
     2281 ctcgataaaa tcattaacat cgaacaactc caataattct gctttatatt tctctaacaa
     2341 tgtatttaaa tagcgatttc tcaattccca tgaactttca ccagttccgt attcaacctt
     2401 gatccctaaa ttcttagtgt agccttttgc tgtgtatcgt ccaatgaagt ctcccttatt
     2461 ccgattgcta tcttcttctt tgaaaaactc acgccaaatc ccctttttct tcttaggaat
     2521 tagacctctg ctcttgaaat catcatattt ttttgaagct tctcttcgtg tatctaagta
     2581 cctttgcttc agctctgtag ctgattcatt cgttccatat tcaacagtga tccttaagcg
     2641 cctgctataa ccttttgctg tatatcgtcc aacaaagtca ccgccacttc gatcactact
     2701 gacttcctta acaaattctc tccatatacc ttttttgtcc tttggtatta atcctcgtct
     2761 tttcatatct tgatattttt cagaagcttc ttttagagta ttcttttttg ctaccatata
     2821 ttccactcac tctttgttat ctagtatttt aataactggt ttcatttttg taatatcttc
     2881 tttttcagct atatacaact ccgttacggt aacgatctta gtatgattta gattaattac
     2941 tttcacttga tcgccaattt tgatttttcc tcgcaaccgt ttgggaataa accagttgta
     3001 taatcgtgat ttattattat ctacataagc ttttattgca ttttcaggaa acaggttatt
     3061 tagtctttca atttttttac ttttaaaaaa ttcattcgca aacttttcat caacaactaa
     3121 attaaataaa gtttcagaat ttacagcttc catatctatt cctttgtaga taggagaaag
     3181 caatttttta tattttgcca gtaatgactt tccagaatta ctaatcttaa tttggttact
     3241 taaatcttct tgataaagaa ccccatatcg aagtaacgcc ttaatataat gttttaatat
     3301 gccgttgata ccagaataaa tatccatgcc gttctcaata tagtagaaag tgtcgtctct
     3361 ccaaaacaat ttgaagctat tattcaaata aagtaacaag atcaatatat gtttgtcaga
     3421 aattctgaca aatctattac tttttagata ctcaccaatg gcttcactta actctttgaa
     3481 gccattgtat tttcccgata aaaatgtcgt aacattatct tctctctttt tcctatggtg
     3541 tgaacttacc ctgaaataat agagtctatt ctcatttaat attgaaaaat aaatactttc
     3601 agttgtttgt gagcattgta tatagttgaa atgatatcca tctggcatga cttttttaac
     3661 tgtattttgt actagctctg accacctttt gttagtagtc atatattttt ttccattaaa
     3721 ccaaacataa atatcttttt taaaaatttt gttccccccc tattgcgttt tctttgacat
     3781 taaagccctt tgttttcatc acattcgtat agccctagcc actcataacc tgatgctttt
     3841 gcgaagttta tttaaaagtg ttttcctttg taatctacct ttcccctcgt cctggtactt
     3901 ttcttggcga ctattaaact attcaacttt tttagaatca ttgtcagctt gaaatccttc
     3961 acgtttcatt atgtcttctg cttttccaat gctgattcct ttccaatcac gccatcctac
     4021 gcttccctta ttttgacgat aggctttaat agctgattcg gatatttgcc atcttgtagc
     4081 caactctttg gctgtgtact tatttgaaaa aagtagcttc tctatcagtg aaatatcaat
     4141 cattttattc tcactcctga aatcttcatt aatcatctct agctagctat atcttcaatc
     4201 aattctaata tgtcggataa accatcaaca gcttcttcaa gttgatcaac ggattcttct
     4261 gctttttcat atctttctga tccctgtaga tttttaggta tattttcttt gtactcttct
     4321 tcttgaatta cttctaactc atcctttagc tcggaaagtc gctcgcttat ttcttctaac
     4381 ctttttcttc ttctttggtt catcaaaatc acctcttcgc ataatctcta cgattttaat
     4441 ataccacttt tgtgtactac attcaatatt ttttgttata gattcgtatt acaaaaattt
     4501 cctctaaaaa aataagagcc tatttaccat ttcagtaatt aagctcttat ttcatttagt
     4561 tgataattct atcaattaac gtacagcaac cgccattcca gtcacattaa atgctctctc
     4621 atacgtgctt gcattgatcc attttgcgcc aaatacaggg tcaacaacat ggtatgcacc
     4681 atttctgaag ccatccacga gaactacatg tccattccat actgcacgac cccaaggcat
     4741 ttgtttgaac tctggcgtag catagttata agtaatccaa gttacaaccg gattaccatt
     4801 gcgaatttca ttcttgattc cattcgttcc tgaaccagtg atattgacgg cattaccgcc
     4861 aacgtttttt gcccatttaa ctactgggtc agccatcatg ccttgatacg ttccattaac
     4921 gttatgacgc cactctcctg agtaaccatg ataaggatta ttgtcattag cttttggcat
     4981 atagtttaca aaactggtta agctttgatt tgtggctaca cctttgtagt gtaaaccttc
     5041 taataaagaa gctccttcgc ataacattgt atttccttgc catacaacag gaacgttcaa
     5101 cactttttca gtgcttggag caggtgtagg atttggtacg ctgtttggca ttgcatacca
     5161 gcctgtacct tcaatcttcc aaccttgttt actaattacg ttcttttcat tttgatctaa
     5221 cgtgtagtga tgccaattca cgtatttgtt atataaacgt gttacaggaa ctctatttgc
     5281 tccgttgtag gaatagaacg caacaccttc gtacgtccat cctgtgtgtg aaatcttatc
     5341 tctttctccg gcatcaactg tgtaaaaatg ttcaccgtta taccgattat acaaacgata
     5401 aacaggagta ccacttgttg gagcttgcca tgcgacacct tcgtaatgcc atccataacg
     5461 tttcagattg tctctttctc caacgcttct agtgtataga tgctctccgc tattgggatt
     5521 atacatacga tacatggaaa tagagctttc tgctgaaact gaaggtgata ataatactag
     5581 ccccattgtg aatagaattc ctactaaaaa ttttttcatt tgattttacc ccttttcatt
     5641 ttttattgtc attattgcat gaaaattatt cgtaagattg ccaattacat aaacgattgt
     5701 tttaagatca taatcgttgt tgt
//
LOCUS       DAXWLG010000152         5684 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXWLG010000152 DAXWLG010000000
VERSION     DAXWLG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.154"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(123..482)
                     /gene="comGG"
                     /locus_tag="ACGE4T_002293"
     CDS             complement(123..482)
                     /gene="comGG"
                     /locus_tag="ACGE4T_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFF8387509.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(454..879)
                     /gene="comGF"
                     /locus_tag="ACGE4T_002294"
     CDS             complement(454..879)
                     /gene="comGF"
                     /locus_tag="ACGE4T_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFF8387510.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(842..1189)
                     /locus_tag="ACGE4T_002295"
     CDS             complement(842..1189)
                     /locus_tag="ACGE4T_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387511.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(1161..1631)
                     /gene="comGD"
                     /locus_tag="ACGE4T_002296"
     CDS             complement(1161..1631)
                     /gene="comGD"
                     /locus_tag="ACGE4T_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFF8387512.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(1606..1884)
                     /gene="comGC"
                     /locus_tag="ACGE4T_002297"
     CDS             complement(1606..1884)
                     /gene="comGC"
                     /locus_tag="ACGE4T_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFF8387513.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(1881..2930)
                     /gene="comGB"
                     /locus_tag="ACGE4T_002298"
     CDS             complement(1881..2930)
                     /gene="comGB"
                     /locus_tag="ACGE4T_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFF8387514.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(2887..3816)
                     /gene="comGA"
                     /locus_tag="ACGE4T_002299"
     CDS             complement(2887..3816)
                     /gene="comGA"
                     /locus_tag="ACGE4T_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFF8387515.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            3948..>5684
                     /gene="ade"
                     /locus_tag="ACGE4T_002300"
     CDS             3948..>5684
                     /gene="ade"
                     /locus_tag="ACGE4T_002300"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFF8387516.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTD"
BASE COUNT         1781 a         1161 c          997 g         1745 t
ORIGIN      
        1 ttctatcgaa caattgacaa aatttcaatc gttttcactg ttttttattt gtgaaacggc
       61 aaacattttt ttattaaaaa cagtgttata acagaaaaga gggcgttttc acgaacattc
      121 catcattctg tactagtatc tgtatcattt tttacttctt ctgtgaaccg aaagataaac
      181 ggtgagactt ccacctctaa gaatagggac tgttgtaccc actgatatgt tacagatcca
      241 gttgtgtagt aaaaaactcc ttccgaactt tcggcaagcg tagactgttc cgataaagcc
      301 atctgcttca ttatttttgc tatataataa tttttgattc gtatattaag atccattgac
      361 aaacggtagc tttctaatgc tgccatcaat aaaaacgtac ataaaagaaa taaaagcaag
      421 acagaaaata aaatagagcc cttataacat tttctactga tcgttttttg tccatatggc
      481 atatccatct tctccatttt caaagtgtac ataaaaagaa attgtgtgtt ctttttccaa
      541 taattgaagc tcagtgatac ttgttaacat aggttgatgt ccgccggaag ttcttctttt
      601 acgtatcatt gcctgatatc tttcgatgat atactgttta tcctcgatcg tgtagtgcag
      661 ttgctgattt ttcacagaga cattgtgact gctcttgagt tcttcttcta attgtgctaa
      721 aaatattagc cactcttttt gatctttacg ctgcaaaaat tcacttactt ggatggtttg
      781 ctgaatcaaa agattgaatg tgagcaatag actgctcaat atcatcagtc caattagaca
      841 ttcaatcagc gtaaatgctg gaattacttg ttttttcaat actgatcgct tctttctcca
      901 tattgacaaa cactttttcg attactccat tattatcgac aatttggata tcgtatggga
      961 tcatttcttc ccttcgctct ggtcttttgt gatacatccg ctgcgtcttg atatcttcat
     1021 aaatcattcg aataaatagc agttcctccc cacttttctt ttctgcctga agcaaaaaca
     1081 gctggccaaa gctcactaag tagacacaga cagaagcgat cataagcgct gtaagggact
     1141 ccaacaaaac aaatcctgtt ttattcaatt ctttttgtat agcgaccact ccctatctgg
     1201 aattgatacg taatcgtccg ctctttttct tcccaataaa attgataagc ttttagtgaa
     1261 ctttcattcc cggttctagc agcaaaagta atagaagcat ggcgtttcaa ggtaatctca
     1321 tcaggaatat ctaatacttc ccaatttatt ttatctggat tcggaacatg aaaaatgatt
     1381 tgattctctt cactattcca ataaaagcct gtttggatct gattcacaat agcgattttt
     1441 tgcgtagaat agattcgttt ttcaaattgg ccgaaaaatt gatagactgc caattcattc
     1501 ttccatgcag aaaatgcgag aactggaaaa gaaaggatcg ttgtaagtaa taaaatcagt
     1561 aagagtgatt caaacaacgt atatcctgat gctaaaacct taccttcaag gccgctgata
     1621 gatttctgca tgttctcgtt tgatatagcc ttcattcaac aattcctcca cagtaggaac
     1681 tcttccatta ttttgaagcg catagagctc tttttgtgtg tctaccaact ggatgatcgc
     1741 ttcacgactt tcttggtcta catgattcct ataacgcgac aaattaggga cgaacaacaa
     1801 cactaataca ctgatgatca gtaagacgat tagcatttcg atcaatgtaa aaccgctata
     1861 ataagatatt tttctctttt tcatggcaaa acctcctcta tgcttccgta aacaggtaat
     1921 aaaacagcgg cataaactaa taaaatcaaa gccgctacgc ccaaaaaaat gattggctga
     1981 ataaaatgca tccagtgatg aagacgtttg aaaagctgtt ttcttgtcca ttcgctgtaa
     2041 aaaatcagtt ctttacctaa acttcccttt gcctctccct gtaaaacaat tttactgaat
     2101 tcttctgata gaaaacgata attctggaac tgctcagcca atgacatacc tgcctccaat
     2161 ccttgaatca acttagaggc aagatgctga atcagacttt cttgtttcgt ttcctttaag
     2221 cagactatga tttgccttaa ttcgaaacct tcatgaaaaa gctttccaat ctctagtgac
     2281 aaataagccg attgatacaa ggaatatatc ggtccaatca ataattgtct gcttatccat
     2341 tcactctgat caacaggaga caacttcatc agacgatacc ttactagtaa agccaataga
     2401 gtaaccgcta ggatgaatag tagaacatac caatgaaaga cttgtatcag ctgaataccc
     2461 caatgctctt gcatgaccat tcctgaacca ataagctgag gcaaaacgaa caaccgcata
     2521 ctaaacaaga tacctagcaa aaaggtgagc aatagcatcg gatatgcaag cagctttttc
     2581 aaatcattgc gaaatccttc aacaagccga aactgtttgg caatttccaa caaagttgtc
     2641 ggcagattcc catgacgttc agctaactcg atctgtacca gttgctctgc tccatatcct
     2701 atttgcgaaa gtacctcact aaacgcttgc ccttccttta gctgatgttg gatatttgcc
     2761 aaaataacag gtggaaacga acggatcgtg agtaataaat aaagggattc ttgcagacta
     2821 aaaccagata aaagcagttc tcccatggtt tgtgcaaact ggttttgctg gcttcttgtt
     2881 aatcctttag aatagttctg actgccaaat attttcctga gcttcttcat aactttcctc
     2941 ccatgatttt tttactgatt cttcctccat aaattgataa ctccataaaa cagaaggatg
     3001 gtgattggca tcttccaaaa gttcttgata aatgacacca gctaaacatt cagatagctc
     3061 ttcttttttc tttgtcagtt catggattct gaccaatgta ccttcaagat tgcgtgcatg
     3121 tacggttgca aaaacccggt gccctgttag tgctgctctt accgcagcat gtgcagttga
     3181 ttcatctctt atctcgccaa tgattagcaa gtctggacga tgccgtaatg cgagtctcaa
     3241 aagtgtatca taagtctgtg ctattttcgg attgatctgt agctgcagaa aaaacggttc
     3301 ttctatctct acaggatctt cgatagtaat cacttgcaat ttttcttcca aagccaaccg
     3361 atacattaga gaagtcttgc ctgatccgac tggtccgctg aaaaggtata atccacgtct
     3421 gacggttttc tgtaaaatag ttgcaaaatc tcgagaacta aagcaacgat ggggtgtttc
     3481 aaaagtaccg tataatagcc gaatcactaa actctctcgt tgaaggtaat ctcctacaga
     3541 agacaaccgc agacgaactt cctgtttttc taaagaataa gtaaatgcac ctaactgagc
     3601 ttttcttttt tctccgatat ccatctgtcc caaatactta aatcttgaga tcagttgctg
     3661 tccatctgcc gaagagactt tcttccagaa tgttcgtttt tttccatagc gaaaatagat
     3721 gtaaaatact tctgaagctg gcaatatata tacgtcttgg gcatgtttgg caaaactttc
     3781 acttatcatt tgatccgata atcgttttat ttccattatt ctcccctcca caaaaaacat
     3841 acgtcttttt gatctttttg tttcaaataa aatgaattta caaaaaaata ttggcaaaaa
     3901 ccgaatttat gttatactta taaaaattta tcttgggagg cactccattg gaaacaacat
     3961 taaggaaata tatcgaagta gcaagtaaaa aaaagcctgc tgacctagtg atcaagcatg
     4021 ctaagatcgt caacgtcttt acgaaagaaa tcatggaaaa agatgtagca atctgcgaag
     4081 gaatgatcgt aggaatcggt gaatacgaag gaaaaagcgt acataatgct aatgggcaat
     4141 atctagtgcc aggattcatc gatggccatg tgcatatcga aagctcactt ttatcaccaa
     4201 gcgaattttc aaaagtatcc ttgcttcatg gagttactac tgtagtgaca gatccccatg
     4261 aaataggaaa cattgctggg agtacaggtc ttgagtttat gatagaagat gcaagacaaa
     4321 cactcatgaa tatctttttt atgctacctt cttgtgtacc tgtaacccct tttgagacca
     4381 atggtgcaaa tttaaatgca aaaaaattag cccctttttt acaaaaaccc gaagttttag
     4441 gcttagctga agtcatgaac tatcaagcag tagcaaataa tgaaacagac atcctagaaa
     4501 agatccaatt gatgcaccag cataagaaaa aaattgacgg gcatgcagct ggtatcggca
     4561 tggaagagtt aaatgtttat cctgcagcag gtattcggac ggatcatgaa gctacaactg
     4621 caaaagaagc taaagaacgt ttggatcttg gtatgtattt aatggttcga gaagggaccg
     4681 ttgcaaaaga tctggcatcg ctactaccgg cgatcacacc tgaaaattca agaagatgct
     4741 tctttgtcac cgatgataaa ctgatcaatg atttagtaaa agaaggatca atcgatcata
     4801 tcgtccgtca agctattgca cttggaatcg atccattaca agccattcag atggcaacat
     4861 tgaatgcagc agaatgtttt gatttgaaaa cactaggtgc gattgcccct gggtatcaag
     4921 ctgatttctt cttaacagac gatcttacca agctgccaat tcatgaagtt ttctataagg
     4981 gaacattagt tgttcaatct gggcaactcc aagaccattt atttgctgat cattcaaatt
     5041 catatacgtg ttgtctgcca aaactaaacg ctcaaccatt aaatccacat tcgttagagc
     5101 tgcttctttc ttcttcacaa gcccatgtga tcgaaattat cccgaatagt ctagtgacta
     5161 aagatttggt agaaaaagtg gaccgacaag gcgaacgatt cattccttca atcgaaaaag
     5221 accaattaaa gattgcggtg atcgagcgtc accatcaaac gggaaacatt ggattaggca
     5281 tagtgaaagg atttcagctg aaacaaggag cgatagcgac aacgattgcc catgactcgc
     5341 ataatctagt tgtcgttggg acaaacgacg aggatatgct gtatgctgct aatcttttga
     5401 ttcaaaaagg tggcgggatg accgttgtca acaaacagca ggaaatagct tgtgtttctc
     5461 ttcctatagg cggaatcatg acacaggaac ctttttcgat cgtctatgaa cagttgaatg
     5521 aattaataac taaagcacat caactaggag cagctaaaac ctttgatcct tttttgacac
     5581 tttctttctt aacattatct gtcatacccg aattgaagat caccgacaaa ggattatttt
     5641 cattttctcg cttccaattg attccacctt gtggaaaaac cgat
//
LOCUS       DAXWLG010000153         5569 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXWLG010000153 DAXWLG010000000
VERSION     DAXWLG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.155"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..784)
                     /locus_tag="ACGE4T_002301"
     CDS             complement(<1..784)
                     /locus_tag="ACGE4T_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8387517.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEI"
     gene            930..1733
                     /locus_tag="ACGE4T_002302"
     CDS             930..1733
                     /locus_tag="ACGE4T_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFF8387518.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1911..2858
                     /locus_tag="ACGE4T_002303"
     CDS             1911..2858
                     /locus_tag="ACGE4T_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFF8387519.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2926..3219)
                     /locus_tag="ACGE4T_002304"
     CDS             complement(2926..3219)
                     /locus_tag="ACGE4T_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFF8387520.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3247..3912)
                     /locus_tag="ACGE4T_002305"
     CDS             complement(3247..3912)
                     /locus_tag="ACGE4T_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFF8387521.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4052..4684)
                     /locus_tag="ACGE4T_002306"
     CDS             complement(4052..4684)
                     /locus_tag="ACGE4T_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8387522.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4691..>5569)
                     /gene="liaS"
                     /locus_tag="ACGE4T_002307"
     CDS             complement(4691..>5569)
                     /gene="liaS"
                     /locus_tag="ACGE4T_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFF8387523.1"
                     /translation="GLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQPIPSAS
                     DDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLARELHD
                     SVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLHLRPVN
                     LEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNTLRHAK
                     ANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVKIISFK
                     GQGTSVETKVPLMKEA"
BASE COUNT         1648 a         1158 c          878 g         1885 t
ORIGIN      
        1 caatctcgct tagtagctga tcttcaaaat caatatgtcc gtcgaaaata attatctttt
       61 cgctatactg ccaaatatgg acatgatgaa cattctttac cccatcgatt gcttcaattt
      121 cttgttccat tttttcatag tcaatagcag gtgcactttg tatcaaaata gcggcagctt
      181 ttttgatcac cattatcgct tctatcaata tatatatcga aatgatcagt gtaataactg
      241 gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc tccgataatc acacttaccg
      301 aagacagtgt atcacttaac aaatgcaaat aactagattt tatattcaaa ctttcatctg
      361 cttcttgtcg taataataat actgagaaca aattcgcgag caatccaata actgctactg
      421 ttagcatcaa atgactattt atcttttctg gtgaagtgaa ccgcttgaat gcttcaacgg
      481 ttaaaaacaa ggataatatg atcaatatga atgagttgat gaaagcactc aatatttcta
      541 aacgctggta gccatacgta cgttttttat ttgcttcttt tcgggccatt ttttgtgccc
      601 cgtaagaaaa aataattgcc aatgtatcac taaggttatg gaaagcatct gaaacaagag
      661 acaaactccc tgacataatc cctccaataa attctgataa agtgatcacc gcattcaaaa
      721 ccactactcc aaatagtttt tttgatgatc tctgtggctg tgtaggcact attccttcct
      781 ccattttatt tatattggat agtctttaga caagaataac atttttttga tatttcacca
      841 atcattcaga tgtttcccta aagcacttgt atttcacttg atacttcgct agaattgcaa
      901 gtgatgacac tactagatga ggagtactta tgacaacaaa acaattaatt atcagttcct
      961 ttatacgatt caaagaccta aaggaggcga gaaatgtccc attttggaaa gccattgtct
     1021 atcttttagt tttgagtata attatggctt tgccaatctc ttttcaagtt tttcaagtct
     1081 taaataacat tcaattggat gggcaaaaga ttgctactcg cattcctgat tttacaatcc
     1141 aaaacgggca gcttcaaacc gaagaaaaag aaggattcat ttaccaaaca aactccatta
     1201 tcttcacatt tgatccagag ggaaaacgaa cagaacagga catctctact gatttgatgg
     1261 ggaattttgt tagtgtcggg atgttaaaaa acaaacttat catagctctt cctaatacag
     1321 gcacgacttc tgctttgttg aacaataacc aattagaact tccctacacc aatgaatctt
     1381 taaaaaactt aacaggtaag cagctccgtt cttttttaag tgaagcatct ataccacttt
     1441 ggataaaagt cctgacattt atattttcta tttatcctag ttttctgaat ctgctgatca
     1501 ctttgctatt taccaacatt gctgcttttc tctatgcacg ctttcgacta acaaaagcta
     1561 cttttcttga ttgtttaaaa acattgatct attcaatttc attgcctact ataattgcaa
     1621 caatcttgat ggtctttctt ccttcttttg acactagcgc cttcattgct tttgcaggaa
     1681 tctttatttt tgctcaagct gtcaaaggat gggcaaaaat atcgatcaat taagaaataa
     1741 agacaactcg ttatttctac ttaaatagac ccttttagag gttgtgacat ttttttgtca
     1801 cagccccttt tttatttgaa aaatatttct ttctcaaaaa acaagcaagg cgcttcaaag
     1861 ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg gaaattaact atgtcagaaa
     1921 gaatcattta tgcagatcgt ccatttcgtg atgaatttat cgaagaaatc catactatag
     1981 cgcctggtta tatctttaaa acagaattac aaccagagga tctacaaaat gtagagatca
     2041 gtttgggctg gaactcagac taccaagaaa aactattagc ctcttctacc ttgaaatggg
     2101 tccaatctat ctcagctggt gttgatcagc taccccttga gactttttca aagaaacata
     2161 ttttactttc aaatggtagt ggcatccaca gtgaatcgat tacggagcat gtcttaggaa
     2221 tcatccttgg gtattctcgt ggtctttttc aagcacaaaa ggcacagagt gacatgaaat
     2281 ggttaggttc atcggtccat taccaatcta tccaagggaa aaaaatacta atcattggaa
     2341 ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc attagacatg aactgctatg
     2401 gaatcaatac atccggtcat ccagcagaag gaatggaaaa gactttttct ttaaatcaac
     2461 taaaggaagt tcttcctgaa gtagatttcg tagtcaatat cttacctcta acggaggaaa
     2521 caactgggct ctttgatcag catatgtttg aacactttga tccaaaatct gtattcatca
     2581 atgttggccg tggtgcttct gttaagacac aagatctggt gcaggcttta aataaccagc
     2641 aactttcctt tgcagcatta gacgtttttg aggaagagcc tcttccacaa gatcatcctc
     2701 tttggaaaat ggacaatgta ctgattactt cccacattgc aggtttgaca cctgattttc
     2761 aaaagaaatt aatggctatt tttttaacca atctgaaaag ctactttgcc acccatgagc
     2821 ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata gctgaaaaca atattttcaa
     2881 aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt gttttttaaa
     2941 gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat atcctttaaa
     3001 gaaccattca tctgcttcct cgataaaaaa aagtgtccca tccatttcca ctttctttac
     3061 aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt
     3121 gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa tttctgagtc
     3181 aaaccatttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct actttctctt
     3241 ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct ctccaataac
     3301 cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga taaccgtttg
     3361 atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc
     3421 taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag aatatttatc
     3481 tgacagctct aagtagtcta gaatattctt tgatactaag ttatgtgcaa tccgtatccc
     3541 catgtctcgc tcagggtgta cgacacgatc tgctccgatc ttctctagta cacgggcatg
     3601 gtactcattt tgggcttttg ctaaaatatt cggtacgccc atttccttta ccatcagagt
     3661 aactaagatg ctggcttgga tatcttctcc aatagcgaca attacatgat cgaaatttct
     3721 tatacctaat gaacgaagtg tcatctcatc ctgtgcattt gccacaacag catgggtggc
     3781 tatattcata tattcattga ctcgatcttc attactgtca atagccagta cttcttgtcc
     3841 agcttcgatc agtgtacggc agatgcttcc gccaaatcgt ccaaggccta caattgcaaa
     3901 attttgtttc accattttcc tcctttagta tcttcttttc tcagtataac aaagcctttc
     3961 caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata gttattttct
     4021 cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt ttaaacgcat
     4081 agatagctgc ttgagttcgg tcttctacct ctaacttagc taatatattc gacacatgcg
     4141 ttttgactgt tttcagtgtg ataaacaatt catccgcgat ttcctgatta cttttccctt
     4201 cagaaatcag catcaaaatt tcattttctc gattcgttaa ttcttcatgc aatactggat
     4261 catttcgccg actcattttt tccatcattt ttgttgtcac ttcaggttct agtactcgct
     4321 cgccgcgttg cgtagccctt atagcatcag ctatttcatg tgctgtagac gtcttcaaca
     4381 aatatcctgc tgctcctgct tcaattgcag gataaacttt ttcatcatca ataaaacttg
     4441 tgacgataat gattttggct tttggccaat cttttaagat tgcttttgtt gattcgatgc
     4501 catccatttc ttccatgact agatccatta aaatgacatc cggtcgaagt gccatcgcct
     4561 tttcatagcc ttgacgtcca ttttcagctt ctccaatcac ttcaatgtct tcttggatag
     4621 ataaataaga agagacgcct aagcgtacca tttcatggtc atctactaat aaaactttta
     4681 tcatctttat ctatgcctcc ttcatcaaag ggaccttggt ctccacgctc gtaccttgcc
     4741 ctttaaaact aatgattttt actgttccac caataccagc tactcgttct ttgatattgt
     4801 tcaatccata acttcccgtt ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga
     4861 tacgtaataa aagattgtta tctattttat gcaaatagac ttctaattca ttagccttag
     4921 catgtcttaa tgtgtttgat aacaattctt gaacgattcg gaacaagtga tcctcaatgg
     4981 aactagttaa ttttacatct tctacctccc attttagcga aatctggatt ttattctgca
     5041 attccttcaa taactgctca atgccttgtt ttaaactttt ttcttctaaa ttgactggac
     5101 gcagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc tctgccacca
     5161 ttttcaactg cttacggaac atttctggtg tctcactttt ttctgcttgc tccgtcaatg
     5221 ccgacatcat catcatcgct gcaaataatt gctgtgaaac tgaatcatga agctcacgag
     5281 ctaatcgatg cctttctaat tttaaaatct cttctttggt ttgtccatct acatattgag
     5341 gacggctagt cataatctgt aattcactgg atacttcgat cattttttct tttattttag
     5401 taatttccat atcaatatct ttgatgtaca gatcatccga cgcgcttggg atcggctgat
     5461 gcaaaagttt ggattcataa ttgccagaag aaagtgcacg aagcttttct tcgattttcc
     5521 cgtattttgc cttttgaata agtgataaca atagaaatac gatcaatcc
//
LOCUS       DAXWLG010000154         5566 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXWLG010000154 DAXWLG010000000
VERSION     DAXWLG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.156"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(57..1196)
                     /locus_tag="ACGE4T_002308"
     CDS             complement(57..1196)
                     /locus_tag="ACGE4T_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8387524.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1505..2218)
                     /locus_tag="ACGE4T_002309"
     CDS             complement(1505..2218)
                     /locus_tag="ACGE4T_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFF8387525.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2472..3173)
                     /gene="nagB"
                     /locus_tag="ACGE4T_002310"
     CDS             complement(2472..3173)
                     /gene="nagB"
                     /locus_tag="ACGE4T_002310"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFF8387526.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3407..4939
                     /locus_tag="ACGE4T_002311"
                     /pseudo
     CDS             3407..4939
                     /locus_tag="ACGE4T_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            complement(5130..5309)
                     /locus_tag="ACGE4T_002312"
                     /pseudo
     CDS             complement(5130..5309)
                     /locus_tag="ACGE4T_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5329..>5566)
                     /locus_tag="ACGE4T_002313"
     CDS             complement(5329..>5566)
                     /locus_tag="ACGE4T_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8387527.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1819 a         1128 c          934 g         1685 t
ORIGIN      
        1 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
       61 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
      121 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
      181 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
      241 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
      301 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
      361 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
      421 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
      481 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
      541 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
      601 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
      661 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
      721 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
      781 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
      841 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
      901 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
      961 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
     1021 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
     1081 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
     1141 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
     1201 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
     1261 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
     1321 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
     1381 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
     1441 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
     1501 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
     1561 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
     1621 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
     1681 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
     1741 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
     1801 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
     1861 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
     1921 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
     1981 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
     2041 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
     2101 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
     2161 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
     2221 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
     2281 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
     2341 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
     2401 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
     2461 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
     2521 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
     2581 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
     2641 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
     2701 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
     2761 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
     2821 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
     2881 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
     2941 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
     3001 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
     3061 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
     3121 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
     3181 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
     3241 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
     3301 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
     3361 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
     3421 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
     3481 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
     3541 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
     3601 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
     3661 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
     3721 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
     3781 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
     3841 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
     3901 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
     3961 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
     4021 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
     4081 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
     4141 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
     4201 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
     4261 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
     4321 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
     4381 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
     4441 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
     4501 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
     4561 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
     4621 aagcagttaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
     4681 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
     4741 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
     4801 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
     4861 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
     4921 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
     4981 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
     5041 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
     5101 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
     5161 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
     5221 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
     5281 ccaataaagt acataaacat aaatacagac ccgtggtgct agttaatttt aatatcctaa
     5341 agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa cactgtaagc
     5401 cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt tgaaattttg
     5461 acaacctaat ttttctaagg aagaaaaaac agtctcaact gtttttcttt gttttccaag
     5521 taagctgtgg tctagttttt ctgatttatc catgttttta cgtggc
//
LOCUS       DAXWLG010000155         5436 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXWLG010000155 DAXWLG010000000
VERSION     DAXWLG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.157"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            224..1327
                     /locus_tag="ACGE4T_002314"
     CDS             224..1327
                     /locus_tag="ACGE4T_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8387528.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(1324..1530)
                     /locus_tag="ACGE4T_002315"
     CDS             complement(1324..1530)
                     /locus_tag="ACGE4T_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387529.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(1520..2587)
                     /gene="rlmN"
                     /locus_tag="ACGE4T_002316"
     CDS             complement(1520..2587)
                     /gene="rlmN"
                     /locus_tag="ACGE4T_002316"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFF8387530.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(2817..5264)
                     /locus_tag="ACGE4T_002317"
     CDS             complement(2817..5264)
                     /locus_tag="ACGE4T_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFF8387531.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         1500 a         1181 c          958 g         1797 t
ORIGIN      
        1 atcggtagaa atactaagag gaacaaaaat tcattattgt cgttttcaat gtttttgtac
       61 ttgttcgttt ttaggatact tattgatgat ttcaaggtgt tcatgccacc ttattcatca
      121 cttatcctgt cttaggttca cctctactcc atctacagcc gcagtcagat cgatcactct
      181 ttaaaatttt aacccgtttc aacgaagaaa gaaggaaatg aatatgagtc aaagtgtttt
      241 cttaccaaat tataccattg gaacagatgc gtatgaaaaa gcaaatggaa tcgtcaaaga
      301 atatgggaca aaagtagtgc tgattggagg gaagactgca ctttcaaagg cagaaaacaa
      361 aataaaagta gctttgacac aggaaataga ggttgtcgcc agtctctggt acgggggtaa
      421 ttcaacttat ggaaatattg aacagttgat ccagcatgaa agtgtccaaa aagcagacgt
      481 cctgtttgca gtcggtggtg gccgtgcttg cgatactgta aaagtcattg gtgaaaaact
      541 agctaaacca gtcttcacct ttcccactat tggttcgaac tgttcgcccg tcactgcggt
      601 atgtgtcatc tataacgaag aaggcgtaat ggacggactc ttctttcctt caaaaccacc
      661 tgtacacacc ttcattgata cagcaattat tgccgaagca cctgataaat acttatgggc
      721 aggtattggc gatgctttat ccaaagaagt cgaatctact ttttctgctc gtggcgatga
      781 tctaggctac acggatagat taggaatcca aatcggaaaa atgtgcattg atcgtctctt
      841 gacttatggt acgcaagcat tgaaagatag tgccgacgat cgtccctcat atgcaattga
      901 acaagtcgtt tcggatatca ttgttactac cggattggtt tctgttttag ttaacaataa
      961 ctataattct gctatggcac actcattcta ttacggtacg actgttctag aaaaaatgga
     1021 gggatacctt cacggagcat ctgtcagtta tggtgtactg accttattga tgatggatca
     1081 gcagttcgat ttgtttgata aaatctatgt gtttatgaaa aagaaccaac ttccagtatc
     1141 gttaaccgac atgaatctca atgtcacgac cgatttagaa gcaatcttag ataaagccat
     1201 ggcaacaaat gatattaaac atgtgcctta tgaaatcacc cgagaaaaat ttaaacaggc
     1261 cattcttgac gtagaaactt atcatgctac acaagaaagc cagcaaggat ataagaaaat
     1321 cagttaaata attgctaaaa cccccatcaa gcaggctgtc atcaaattga ggacatttct
     1381 ttgcttgatg agggtttttc ttgtcaaaaa aagctttata gctttcgcta tagaatcttt
     1441 tctcttattt aagttgaaca gttccttaat taagaaaact gctcctttta tggtaattga
     1501 aaaaacgagt aggatacgat tatttggcaa ctgttttttt catttgcttg cttcgcaatt
     1561 gtccgcaagc tgcatcaata tctgtaccat gttctttacg tatcacacag tttacgccgt
     1621 ttttcttcaa tacatcataa aatctcaata catcagcttt tggactgcga gaatattgat
     1681 catgttcact gactggatta tatggaatca agttgacata tgctaatttt ttcttatctt
     1741 ttaatagatc tgctaattgt tgcgcatgct ctggtctatc atttacttga ttcaacatga
     1801 tatattcaaa cgtgattcga cggtttgttt tctctaaata ctcatccaca gcttccatca
     1861 atttctcaat tgggaaactt cggttgatcc gcatgatcga tgttcgcacc tcattattcg
     1921 gcgcgtgcaa ggaaatagcc agattgactt gcaagccgtt attagcgaat tccttgattt
     1981 tgtgtgctaa tccactagtg gaaactgtaa tgtgccgagc accaattgcc aatcctttgg
     2041 catcattgat gatatgcaaa aagtccatta cattgtcgta attatcaaat ggctcaccga
     2101 tccccattac aacgacatgt gaaacgcgtt cgccaagatt gcgctcatca aaataatgtt
     2161 ggaccatcat gatctgtgca acaatttctc cagcagtcag atcacggttc tttttgagca
     2221 gcccgcttgc acaaaacgta cagccgatat tacatcctac ttgtgtcgtc acacaaactg
     2281 acaagccata ttcttgtctc atcagtacag tctcaatcat gtttttatca gggagttcaa
     2341 acaaatattt tactgtacca tccgaagctt cttggacgac catctgtttt agaggattga
     2401 tgataaaatg ctccgataat ttttcgatca attgtttcga tagattcgtc atttctgtga
     2461 aattacttac acgtttttga tacaaccatt cccaaacttg ggcagctcgg aattttttct
     2521 cgccgttttc tataaaccaa ttgattaatt cttcattcgt caagccataa atggactgtt
     2581 tttccaaagt attttccctc tttttctcaa agtttcgcta catactatac atgattcatt
     2641 tccttaatgc aatacttcac aaataaatcg aatagaactt taatctcaca aaaactaaaa
     2701 agacaagaca tcataccgta ttagaaaaga tggaaaaaaa ccattttttc ttttggtatc
     2761 atcatgcctt gtctttttat tggttattcg tttttcaacc cttcatcaca caagttttac
     2821 atgtcattat cgtccggcaa cgtttctgat gtttcttcct gctcaatgaa tactttccga
     2881 ggcttgcttc cttctgacgg gccaacaact ccatgagctt ccagttcatc tactaaacgt
     2941 gctgctcggt tgtaaccgat ccgaaatctt ctttggagaa gggaaatact tgccgtttgc
     3001 atttcaacga caagtgcttt tgcttcttca aacagctcat cttgtggctg ttctggagca
     3061 cctgtcgttt caacttcatc tgtcggcatc attttctctt cataatgagc ttcttgctga
     3121 tctgtaacaa attgaacgac ccgttctact tcgtggtcag aaatgaatgc tccttgcaca
     3181 cggatcggtt tattctcacc cattggcaag aatagcatat ctcctcgccc taagagcttt
     3241 tctgccccat tagaatcaat gatcgtccga gaatctgtac cgcttgatac cgcaaatgcc
     3301 attctggaag gtacatttgc tttgatgatt cctgtgataa catccacact tggacgttga
     3361 gtagctagga tcatatgaat ccccgctgcc cgagccattt gcgctaaacg aataatcgca
     3421 tcttcaactt cattgctggc taccatcatc aaatcagcta attcatctac gatcactacg
     3481 atgaatggta aggttggatg tttcgttccg ttttccaagt ttttctgttg aacaaattca
     3541 ttgtaaccag aaatatttct tactccggtt gccgcgaatt tttcatatcg ttcttccatt
     3601 tcttgtacta ctttttgtaa ggcttgcgct gcttttctag ggttcgtcac gaccggcgtc
     3661 aacagatgag gtatgccgtt gtacatattc aattcaacca ttttcggatc gatcatcatc
     3721 agttttactt catggggctt agcacgcatt aatatacttg tgataatccc attaattgct
     3781 actgacttcc cactaccagt cgaaccagcc acgagtaagt gaggcatctt cgaaagatca
     3841 gccgtttgta cccgtccaga aacatcacgt cctaaaggta cttctagtaa tttatctggg
     3901 tggtttggtt gtgcttcgat gatttctcta aaggaaacca tactgatctt tccatttggt
     3961 acttcaatac caatcagtga tttacctgga atcggtgctt ccatccggac atctttcgcc
     4021 gctaaagcaa gcgcgatatc atctgttaag ttcacaattt tacttacttt tacccctaca
     4081 gccggctgga cttcgaattt tgtgacggat ggtcctaagc tggctttgac gaccttggca
     4141 tcgacaccga aactttggaa agtttgttct aatacgccga tatttttttc aattttttta
     4201 tactcatcac tttgatccac agttggtata gaatcaagca aatcaactgt cggtaattca
     4261 tagtcttgat tttcctgttc tgcttcaatc tcaaattcta atgaggtacc gtcatcctct
     4321 ggcaactcat ctgaaaaacc ttctgctgta ttggtctgtt ttggtgtatc tggtactggt
     4381 ttctctagat tcgcaggatg gatattttct tgaaaactgt cgattggaac aaaacttagc
     4441 tgctccggct ctgtattttc tggttcctgc cattcatcag cagtattttc cctttgctgg
     4501 ctcaccgctt ttttcttctc atattctgca gcttcttttt ctgcagcttc ttttgcagct
     4561 aagcgccttg cttctttcgc ttctgcgcgc tgtttcattc tctcttcctt tgccgcttgc
     4621 tttcgagctt gcttggcagg atcaccttcc aataattcct gtactcgttc tccaattgat
     4681 tggataccat ttaacagctg atagccttcc agattactga acaggaatgc tcctcctgcc
     4741 agtaatagca ctgctgctaa ataacttcca ggctgtgcaa tcaagaagta agtaaaatga
     4801 taaaggattg ctccaatcat cccaccacca acgttattgt ctacttgact ttgttttata
     4861 tcactggcta acaaatccca tgtcgtcccc atgatatttg gttccccagt atggagtttg
     4921 ccaaataaat gggcatgtaa caataataag ataccgaaat aaaagagtat gccgccaaac
     4981 cagcgtcggt ttttaccaat tgaaaactct gtattcttaa tgacaatcca aaatcctaat
     5041 acggctaata aaagacagag gatttggtaa gtatttcctg caatgatccg aaagccgttt
     5101 gcaatcaata ttcctaaaaa acctaatttt aaaaagccga agacagcaaa taaaatcaag
     5161 gcaaacccaa caccaatcgt cagcatttgt tcctgctgct gtttttgctt tttcgttttt
     5221 ttcgtctttt tccttttttt agtaggccgt ttcttttgtg ccatttatta tccctcattt
     5281 catcaagttt cattatacaa aaaaaagtaa gggaactaaa gttttgtttg aaagaactga
     5341 cgctttcttt catttttcag cttattttac agtttttctt aatttctaaa tgattttatc
     5401 aagaaaaggc acggccatat ccagccgtac cttctt
//
LOCUS       DAXWLG010000156         5436 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXWLG010000156 DAXWLG010000000
VERSION     DAXWLG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.158"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..684)
                     /locus_tag="ACGE4T_002318"
     CDS             complement(<1..684)
                     /locus_tag="ACGE4T_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HFF8387532.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTT"
     gene            810..1220
                     /gene="tagD"
                     /locus_tag="ACGE4T_002319"
     CDS             810..1220
                     /gene="tagD"
                     /locus_tag="ACGE4T_002319"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFF8387533.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            1594..1809
                     /locus_tag="ACGE4T_002320"
     CDS             1594..1809
                     /locus_tag="ACGE4T_002320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387534.1"
                     /translation="MIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYLVAPSCFIS
                     KQVHYTILEELTERQALKTIAKRCDFQ"
     gene            1966..2223
                     /locus_tag="ACGE4T_002321"
     CDS             1966..2223
                     /locus_tag="ACGE4T_002321"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8387535.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            2310..3548
                     /gene="istA"
                     /locus_tag="ACGE4T_002322"
     CDS             2310..3548
                     /gene="istA"
                     /locus_tag="ACGE4T_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFF8387536.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            3538..4278
                     /gene="istB"
                     /locus_tag="ACGE4T_002323"
     CDS             3538..4278
                     /gene="istB"
                     /locus_tag="ACGE4T_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFF8387537.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            4697..4852
                     /locus_tag="ACGE4T_002324"
                     /pseudo
     CDS             4697..4852
                     /locus_tag="ACGE4T_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4919..>5436)
                     /locus_tag="ACGE4T_002325"
     CDS             complement(4919..>5436)
                     /locus_tag="ACGE4T_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFF8387538.1"
                     /translation="TKEFVPWFFGENFKPMTINMLISCLIIVVSPIGGVFANQFAIAI
                     EKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLVFFFRVFFVRKEIKI
                     SYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMIFIIYLPFVYFLFPD
                     IRNEVRKIIRK"
BASE COUNT         1904 a         1011 c          940 g         1581 t
ORIGIN      
        1 tgtggtgcgt gaacccgata aagcaataaa cgagaattga ataaaaatat taattacaaa
       61 aaatatttta aatagaagat ttaaatgctt gtaagtgacc aaataatata tagcaataat
      121 gatgacgatt aatgatataa ccgcaccaaa atttggatct gtatatacac caaataaacg
      181 gttgcctaac caccctaaac gaactgggta atacttatat tttattggaa tttttatgcc
      241 atattgaatg acaaaccagt ataatgatac taaattagct aaaaaccata ccaatgaaag
      301 tatccaggta aatactttga aaaaaaagtt actactatag tctttgccaa actgataaat
      361 cataaagaaa aagattaatt gccacatgat taacttaata ttttcgctat atccatattt
      421 taaattcatt aaactgctta ctagcatcgc gccaataaaa gcaattaata gccaatcata
      481 tttttttaga tttttttctc taaatgcaat aattgcatct ataatgatca agacaactcc
      541 cgctattcca gcaggccaaa aaagtaaagt atcatatcta gtaggtaata gtaaatacgg
      601 gcctattgct cgtaacatta aaataaaacc aaaataaata aaataccaac ctttcacata
      661 ttttaaaaat ctttcattca acatttttcc tccatccacc tatttctcat aaatatttct
      721 tatccttttt aggataaact ggtcctttca attctaacaa aaaaatgcta aactaacaat
      781 gtatattaat gaatttcgag aggtgaaaga tgaaaagagt aattacttac ggtacctttg
      841 atttgcttca ttacgggcat atcaatttac tgaaacgagc aaaacaatac ggagactatc
      901 ttattgttgc cctttctaca gatgaattca attggaatgc gaaacaaaaa aaatgttatt
      961 ttagttatga aaaaagaaaa cagttacttg aagctattcg ctacgttgat ttagttattc
     1021 ctgaagaaaa ttgggaacaa aaagtaaaag atataaaaga ataccatgca gattatttcg
     1081 tgatgggcga tgattgggct ggacattttg atgaacttga acaactaaca gatgcacaag
     1141 tgatttattt accaagaaca cctgagatct ctacaactca aataaagaac gaacttaatt
     1201 ctttaaatag tgataaataa tattttttaa acaataaaag aacaatatat ctggataaga
     1261 cgtaaaaaaa tggctctata atttctagga ctgatagaat tttctgtcgg tcctagattt
     1321 tttgtaaaaa aaaacagaca taaaaaaatt ccgatagaat agagtcgtct aaaacatcaa
     1381 tctgaaagga agtttttgat atccatttct cattctatca aagagctctt cactattaaa
     1441 gataaaaata ttgaaattaa ttcttttcaa gaaacttttt ataaggacaa gaaagccatc
     1501 gtaatcgaag gtccattagt tcgcactttt agacattgtc tattttgtaa agaatctgca
     1561 aagcagatcg agaaaaacgg caagaagatt tcaatgatta tcttaaatcg cagtgggaat
     1621 aaacaaacct atttgcgtct tagaaaacaa cattaccact gccaatcatg cgggaaatat
     1681 tttactgctc atacgtatct tgtcgctcct tcatgtttta tttcgaaaca agttcattac
     1741 acgatattag aagagttgac cgagcgtcaa gcattaaaga caattgcgaa acgttgtgat
     1801 tttcagtaac gacagttcaa cggacattat ctgctctatc ggcaacgctg cgacagaagc
     1861 tagactattt acctcattgt ttactattcg acgagtttcg ctctctttca attttccatg
     1921 ggaagttctc cttttcttgt atgaattgag agacagggaa gctctttgac attttgtcca
     1981 gccggagaaa aaaaagattt gattgcttat ttcatgcgtt ttacaagaaa agctcatatg
     2041 ggggttcgat acattgtcac agatatgaat gccccttatt tttcattagt caaagaaccc
     2101 ttcccaaatg cacaaatcat cattgattgc tttcacattg ggcaacactt gaacaagttt
     2161 ttcgatcgtg ttcgaaaacg tgtgatgaag cagttgaatc aaaaatgtca acggcacctt
     2221 taaaatgtag gaaaacggca atttaaaatt gttggtttac ctacattttt gtatgctctt
     2281 cttatcccaa atgaaatgga ggctcaggga tgagaaaaga catacgcgaa ggagtgatga
     2341 tctacgtgat caatgaaatc aaacccaatt atgctgcact agcaaaacaa tacgactgcg
     2401 actaccgaac agttaagcat gcttacgaag aagcccaagt taaagaatca aaacctccag
     2461 aacgtaagaa gcgtccaagt aagttagatc cctacagaga aatcatccaa gacaaaatca
     2521 aagaccaatg cagtgcttac agcattttca agtttattga gcacaaagga tttggaggaa
     2581 gctacagttt agtaaagacc tattgtcgcc agattaagca ggcgaaagta cacaaggcaa
     2641 ccgtccgagt cgaacatagc cctgggcttt ctgctcaggt tgactggaag gaagatatgc
     2701 gactaatcag taatcagggc gaaatttttg agttcaatat ctttttgtac gtcttgccat
     2761 attccaagaa aaaatatgtc acactaactt tcgaccgaaa acaagacaca ctctttgagt
     2821 gcttagatga tgcgttttat caaaccggtg gcgttcccaa agaaatctgg ttcgataata
     2881 tgaagacagt cgttgaccat tctcgtagtc agttttccaa accgcatttc aacaaacgtt
     2941 tttatgcttt cagtaaggat gccggcttca caccaattgc ctgtcggcca tttcgtccgc
     3001 aaacaaaggg ctgtgtcgaa gctttagcca gaactgttga ccgcctacgg gtctataatc
     3061 atgaatttta tgatgccgtt gagctgtgtc atttagtgaa taacctttgt gttgagttaa
     3121 ataacgaggt ttctcaatca actgatcaaa caccagatga acgttggcag acgaacgaaa
     3181 aagagcacct gcatccatac caaaaaaacc ttttgaatcc ctattttgaa gactttattc
     3241 gccgtaaagt caccaaggaa gaaatggtca ttttccgtaa atgcaggtac tctgtggcgc
     3301 ctcgctatat cggtaaagaa gtcgaaatcg aactctccga taaagaagac caagtacaaa
     3361 tctattataa cggagaactg attcggtcgc atccattaac gacaaaacag ctgaactacc
     3421 acgaggaaga ctatttccaa atcttgaaat ccgatgtcat gagtcacaaa gaagacgatg
     3481 gaatcagagc gtatactcgt gaaagtctca agcaatatga tctggtggag gttgccgatg
     3541 aagaatagcc attaccaaga actgatgacc aacttagaga ctcttggttt gaatcatatg
     3601 cgggaatatg ttcctaacta catcgaagtg gccaatcgag aggagctttc tttaacggaa
     3661 gctcttctcg agctgacacg cagagaagtt gatcatcaaa ataagcaaaa aatgaatcgt
     3721 gtggtggcac gcgcccgctt ttccaagaga accagcctgg aagagtttga ttttgccttc
     3781 cagccaagta tcaataagcg cgaagtgcta gacttaaggc acatgggctt tgtggaaaaa
     3841 catgagaact tggtttttat cggcagtcct ggtgtaggca agacacatct gacgattgca
     3901 ttgggaatcg aagcctgtcg tcaaggctat cggacactat tcatcacctg tcatgagctg
     3961 cttttgcgtc tgcgaacagc ctatgaaaaa ggtaccctag agcgtgttct caaacgatac
     4021 gctcgctatg acgtcttgat tattgatgag attggctatc ttccgattca aaaacaggaa
     4081 gctgatttat tctttcagct tcttgccatg cgctatgaac accgctctac gatcattacg
     4141 acgaacattg tcttatctcg ttggggggag ttgttccaaa gttctgagat cgcagctgcc
     4201 atccttgacc gtttagtaca tcacgtcaag gtgttcaaga tcacaggcaa gtcttatcgt
     4261 atgagagaca agatctaaag tgtcgattgt cgacaattac tattgccgaa aacctacatt
     4321 tttatcctgc ccaaaaccta caattttaag ctgcccttga catcaaaaag acacttcaca
     4381 agctaagtgc tatcgacaat tgaaaccttt gtataaatta cttctataat cagatgacga
     4441 gttggattat acaacattca agaaatggca gaattttcaa tggcgctatt taacagaaag
     4501 tgaagtagtt gattgtctac cctcaatctc ttatgaactt aaaatggctt agcagtatta
     4561 tcaaaagctg cttaacaatt atcacaagaa acagctagct acttcgagaa atgaccaaag
     4621 aagttccctc gaaacttcac tatcttaaaa agcctttttc atatctgaaa aaggctttta
     4681 actagcattt tcgaaaaagt attttaatac aaaattagag aatcttcgca cacatatcaa
     4741 aacattgaaa cgatttgctt atggattttg cagttttacc aatatgcgct tgtggatttt
     4801 tctaatcaac gggttaataa gctatgcatt aaaagctagt aaatcttact aactttgttg
     4861 atcataaatg accttatcag cccgatttga caaagaacta cttttttaca acattatttt
     4921 attttcgaat aatctttctt acttcatttc gtatatctgg aaaaagaaaa taaacaaacg
     4981 gaagataaat aatgaaaatc attattgatt taattgccat atctaaaaat gaagaagaga
     5041 ataattgtgg aagaaagtaa taggccgaag tagtcaaagt aagattgact ataaaaaata
     5101 agacaacgtt cttcccaaag aaataggata tttttatttc ttttcgtaca aaaaatactc
     5161 taaagaaaaa gactaataat tctactacca cgagtacgat tgtagccgct acagcgccat
     5221 aagtaggcac aaaagctaca tttccaataa tacttacaat cgcaccaatg atcattggat
     5281 aagcatactc cttatctttt tctatagcaa ttgcaaattg gttagcaaaa actccaccaa
     5341 ttggactaac aactataatt agacaagaaa taagcatgtt tattgtcatc ggtttgaaat
     5401 tctccccaaa aaaccatgga acaaattctt ttgtat
//
LOCUS       DAXWLG010000157         5433 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXWLG010000157 DAXWLG010000000
VERSION     DAXWLG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.159"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            11..736
                     /locus_tag="ACGE4T_002326"
     CDS             11..736
                     /locus_tag="ACGE4T_002326"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFF8387539.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            726..1400
                     /locus_tag="ACGE4T_002327"
     CDS             726..1400
                     /locus_tag="ACGE4T_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFF8387540.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(1688..1972)
                     /locus_tag="ACGE4T_002328"
     CDS             complement(1688..1972)
                     /locus_tag="ACGE4T_002328"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFF8387541.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2266..3573)
                     /locus_tag="ACGE4T_002329"
     CDS             complement(2266..3573)
                     /locus_tag="ACGE4T_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFF8387542.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4062..5168
                     /locus_tag="ACGE4T_002330"
     CDS             4062..5168
                     /locus_tag="ACGE4T_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387543.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         1756 a          944 c         1040 g         1693 t
ORIGIN      
        1 gagatagtat atgaacaaaa aaagatttaa acgtattgaa agaaaatttg cattaactta
       61 tgaccaagct aagtatttga aaacactatt tccaaaacag gtagttcggg ataaatatag
      121 tgatcatttt atttcttcct tgtattttga tacaaacgat catcactttt taaaagaaat
      181 cactacagaa aagtcctatc gagaaaaatt tagaatcaga aaatatggag atcctcagga
      241 catatcgcct atctacttag agataaaaag aaagtttcaa ggaatgacag ataaacgtag
      301 aataaaatta gcgcatcatg agctcgatga tatgttggaa aatatcgaat tggcagaaac
      361 cacattcagt aatttagaag aagagtatat ttttaatgaa atacagtggt tgttcagaag
      421 gttggcattg gagcccaaag tatatatcta ttatttaaga gaagcatttg taaatagaga
      481 ttcagaagat ttgcgaatca cttttgatca aaatatccat taccgtctaa tgaacttaac
      541 gacctgggaa cactgtacag aagacaatct ggtagcgcca gaaatcgatg tgctgatgga
      601 agtaaaaacg acaggagaaa tacctgaatg gttacaggta ggattggata ggatgggtaa
      661 aaaagagata aaattttcta aatataaaca agtctataaa agatatatcc aagaggaccc
      721 gctctatgtt caatagtgtc ttaaaaaatg agccttctca actcacacta gatgatttct
      781 ttttatgtat ggtggtgtcg attttgctag gcattctgat tgcttacacc catatgtatc
      841 aaaacaactg tacaaaaaac ttcatgtttg ccttgattgg gttacctact ttagtacata
      901 ccgtcatttt gttgatcaac ggtaacttag gcacggggat cgctgttcta ggcgcattta
      961 acttagtgcg atttcggtct gctcaaggga attcaagaga tattttcagt attttttggg
     1021 caacgtcgat tggtttagcc acagggacgg gatatattgt ctatgcacta ttcgtgtcta
     1081 gtatagttgc tgctatccaa gtcatcatct tcacgcttcc aatcaaaaat aaacatacag
     1141 tttttaatac tgaaaaaacg atcaagctaa aagtttctaa atccttgttt ctttcgagta
     1201 ttattgaaga aaaaattgca gaatatacag aaaactgcaa tttaaaaaaa gtaaaaatca
     1261 ataaagaaac ttgttcctta cactatatag taaaagtaaa aacgagtatg cttgaaaaag
     1321 aattggttcg aacgatcatg gatcatccag aagtcatttc gatcactgta ggtatggata
     1381 aatcgaataa attattgtga taaccaagaa agtacttaac aatgaagtaa gaccccagtt
     1441 ccaaagttta gcggggacac ttcaataaag tactttcttt tttgtcttca gcaactactg
     1501 gtaaaactga gagcaagctt tcgtgagagt acagcgataa agatcaacct ccctttttat
     1561 ctcgagcaat ttccttgcaa ggctgtttcg ttgatcttaa acgagttagc aacagtcaaa
     1621 gatgcgatgg ccataaaaaa attgcattaa gttggctggt atctgatagc tgggaatcta
     1681 aggaagacta tccattctgt ttcttaaatt ccgctagttg atagatatca gccaattgtt
     1741 ccagatgatc aacatcaaag ataaccgaaa gatgtttcct tttattccca tttgcttgaa
     1801 gagtttttat cttctctttg gttccttcac tcaatgagag tgtgactaat ttctccctcg
     1861 ttttttctgg gaaatgaaag gatactttga aaaatccgtc ccaaatagaa agccagaaga
     1921 tcggttttac tttctgagag tgggacaaaa gtaagaaaca cttttgtctc attctttaaa
     1981 aacgaataaa cggtgggagc agaagcaact ccttcggaaa taagccaaaa ttcacaaaaa
     2041 tttgaaaagc aattttcgtg aatttcttct tatttctcgg agttaaacac ttctgtccca
     2101 acctcttgta ttcacctttt gatagccaag cttttccatc attataaaac cgcaaatttc
     2161 aaaaaaaaac gaacaataca aaaagcgtat tgctcgtttt tctcttcagt ttctcacacc
     2221 taaacgactt ttgtctcgac ctctttttgg tgactattca tgatgttaaa ataaattact
     2281 gaaaaaatct gtgacatggc gccaaccaat gacaaaaata ttagcttttt caacagtaga
     2341 agtggtaatg atgtctgtcg aaggctcttg gtttgcttcc aaatagccta atttgtcatc
     2401 agctaattgg atcgttgctg agccaactgt agtgcctttg ttgataggag cggtcacttg
     2461 gtcatccgtt actttctttt tatcaattgt tggagtaatc gttaattttc cagtatccat
     2521 tccattttcg acccagactt ttacagggct ttttagcgca acattgacgg aggtttcttt
     2581 tccatctttc actttgatgt cttttaggct tgggatgctt gcatttgctt ttccaagttc
     2641 tttttctgac cagttgtcat aacaataatc catcaatcgt gatgtttcat tgaatcgggc
     2701 acttggattt tcttcatggt tatctgcatt taaaacgact gtgatgattc gtcggccatc
     2761 tttgttgatc gttccgacaa agcatgcacc cgcaaaatca gttgtgccag tttttaatcc
     2821 atcgacacct tcgtgatagt ttacaaaacc aggtagcatc cagttccaat tgaccatttc
     2881 aaccggtgac tgtgtgtttt cgccgaatgt ttgatttgtg gtactcgtga tttttaagac
     2941 ttcaggataa tctaagatga ggtgacgagc aacaatagcg acatctttcg cagacagttg
     3001 attttcatct gtttcaccag tattctcagg tcgattctca cctaggtaag aattgtttaa
     3061 acctgatgca ttgacgatcg ttgcatcttt gataccccaa tcttttagtt gtttcttcat
     3121 tagctcaaga aatttacttt cactgcctgc aactttttca gctaaagcaa ccatggaagc
     3181 attggctgat tggatgaaag ctgagtcaac aagttctttc actgtataat cattttcttt
     3241 atgcaaagga acattcgata gatccggcac gacacttaat ttttctgcat aatccgaaat
     3301 ggctacctta tcttcccaag ataatttacc ctctttgatt tgttcaagta cgatatatag
     3361 accaatgatc tttgtgatgg aagcaattcc catcgttttt tcgccatcct gatcataaag
     3421 gatttttcca ctttctgcat ctacggcaaa ggcagctttg gcatttattt taaatgcgtc
     3481 gtctgcttga acagttgcgc taggcaagaa agaagtgata cataagataa gacttgctaa
     3541 tagtacaata aaacgggtat tttttttgaa catctttgtt ctcctcattt ttaaattgat
     3601 tcttacaact tagaatatta tcagagagaa caaaaggttt caatggttct gaaaaaaacc
     3661 ttataaagaa caagtcttat taatgaagaa tcatagaaag ttatcttttg ggttcattga
     3721 cacgaacttt gactggctga acttcgggtt cggttaggcg tgatgctgca aaaagggcgc
     3781 ctgctgagag tgcgaccaaa ctaaaaatca tgaataattg cataagatca cctcgttctt
     3841 ttttgttttt ttatagtata gcaagagagt ttgaagctac tgttaaggaa atgagcatat
     3901 aattgatgaa agtatgaaaa aaatatgaag taggacaaca caactaaaaa tcgatcaggt
     3961 atttctctgg tagggattcg ttttattgta ttgataaaat gggatgtggt agactttatt
     4021 tacagataca aagaaatgat ttttttagga ggggtacgtt tatggcaaaa aaagaaaaag
     4081 aattattatc acaattgact tcagaaggta ccaaaggacc gtttgtcacg attatgttaa
     4141 acacccatgt tgcgcatcaa gacgtagaaa aagatcaaat caaattcaag aattttgcaa
     4201 aggaagcgaa aaagcgcttt gaaaagaaat actctgagca tgattggtct ccattccaag
     4261 aaaaaattac tagtttgttg gacgatccgt ctttctggcg tagtgccaca acgagtgtag
     4321 ctgtcatagt atcaccagaa caagtgttta tccaccgttt gggtattcct gtagatgacc
     4381 agtattatgt gagtgatcta ccttatttat tagcgatcat caaaaatgga catttcaact
     4441 attcatacta tttattagga ttgaatcgtg attcgatgaa attgtacgaa gtaacgaata
     4501 aacaagtcaa agaagtggct ttgcctgaag atgcaccaag agatgtcgtc actgctttag
     4561 gatctgaact aacaggaggc gggttgaact acgcatcaaa tggtagtgaa ggtacgacgt
     4621 tccatggagt caatcctaaa gatgaagaag tcgaaattga ttgggtcaat tattatcaag
     4681 cagtcgatga ctatttgaaa gatacatttg aaaatccaga gaaatggcct atcttcttat
     4741 ttgcattacc agaaaatcag acgatgttca aaaaagtggc gaagaaccct tattatcgtt
     4801 ccgatgttgc gatctcagct tcacctgcac aactttcatt aaaagaaatt gaagcaggcg
     4861 caaaaaaact atcgcaagaa ttagctgtca aagaagcaag tgactacaat aagttgcttg
     4921 ataaaaagtt cattgatcaa ctcgttgata ttataccggc agcaaaagaa gggaaaatat
     4981 cccatttatt cattgctaca tctaatttag cagatggttt tggcgaagat ccagaaacag
     5041 aatatgaccg ccgtcaggtg ctgaatgaac tagcagataa tgtgatcacg aacggtggtc
     5101 aggtctttat ccttgaacaa aaagatgcac cagacgaaaa gagtttagtt gcgatcttac
     5161 gttattgagt gaaaactacc aaagtagatg aatggtcagg cgttgataga agtgtatagc
     5221 tccgagagaa ggagtttcct ctgtttcagc cgttggattc atcttgagcg agagacaaaa
     5281 gctagctttt gtctctcgct cattattttt gtgctttagt cccattggct ttgcgagggg
     5341 aagatacttt tctcaaaaaa acgaaccttg cagttgagag ggggatagat gcgcatacta
     5401 atttttatca gttaaaaaat gatgtggatc att
//
LOCUS       DAXWLG010000158         5414 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXWLG010000158 DAXWLG010000000
VERSION     DAXWLG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.160"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            27..845
                     /gene="purR"
                     /locus_tag="ACGE4T_002331"
     CDS             27..845
                     /gene="purR"
                     /locus_tag="ACGE4T_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFF8387544.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1348..2721
                     /gene="glmU"
                     /locus_tag="ACGE4T_002332"
     CDS             1348..2721
                     /gene="glmU"
                     /locus_tag="ACGE4T_002332"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFF8387545.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2815..3786
                     /locus_tag="ACGE4T_002333"
     CDS             2815..3786
                     /locus_tag="ACGE4T_002333"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8387546.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3792..3894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3960..4529
                     /gene="thiT"
                     /locus_tag="ACGE4T_002334"
     CDS             3960..4529
                     /gene="thiT"
                     /locus_tag="ACGE4T_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFF8387547.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4610..5383)
                     /locus_tag="ACGE4T_002335"
     CDS             complement(4610..5383)
                     /locus_tag="ACGE4T_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387548.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1769 a          962 c         1155 g         1528 t
ORIGIN      
        1 aaatttctta aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca
       61 tgacacagta tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac
      121 gttatgaatc tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta
      181 aagaacgagg gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca
      241 tccctgaaat ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat
      301 cagaacaaga ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac
      361 ctgaactgct gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg
      421 atgctgtaat gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc
      481 tgaatgcgcc attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa
      541 gtgtcaacta tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa
      601 gtttgaaacg tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag
      661 tcaacggaat gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat
      721 ttgccgaatc aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg
      781 tcaaagatgt agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag
      841 aataagaggt tgtgaaaaag ccgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
      901 gctttccaca ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt
      961 ttatctagag gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa
     1021 ttgctcctca tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg
     1081 tttaatgaag aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa
     1141 aacagctttt catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca
     1201 ccggttacca aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg
     1261 ggataaaaga aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc
     1321 agcaaactat atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca
     1381 gggaaaggta cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa
     1441 cctatggtag aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg
     1501 atcgttggac acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg
     1561 ttacaagcag aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa
     1621 ggaaaaaaag gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg
     1681 ctgaacaatc tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca
     1741 caagcagaaa acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa
     1801 aaaatcgtgg aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca
     1861 ggaacttact gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac
     1921 aatgctcaag gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat
     1981 acagttgctg cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc
     2041 gcgttagcta aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat
     2101 ggcgtaagct ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca
     2161 gacacattga tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt
     2221 gtcatcgggt cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa
     2281 aactcagtca ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat
     2341 ttacgaccaa aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa
     2401 aatgctcaaa tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg
     2461 ctgggagaag aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac
     2521 aaacatcata cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga
     2581 cctgtcgagg tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt
     2641 ccagaatatg cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa
     2701 aaactccctt atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga
     2761 ctactattta ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca
     2821 aaacattact ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca
     2881 gagaaaattg cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt
     2941 gatggagaaa ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt
     3001 caatcaacga gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca
     3061 ctgaaacgag caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt
     3121 caagaccgta aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt
     3181 caaaaagctg gtgcaacaag aatgctgact ttggaccttc atgcagtcca aatccaaggt
     3241 ttctttgata ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta
     3301 gaaaaaggaa tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca
     3361 cgggcacgta aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt
     3421 ccaaaagcaa atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt
     3481 gtactgatcg atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg
     3541 aaagaagctg gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct
     3601 gctttacaaa gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat
     3661 cttccagaag atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga
     3721 gatgcaatca aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat
     3781 aaataataaa taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa
     3841 acccttcgta cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac
     3901 tattttcgaa agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta
     3961 tgggaaaaaa tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt
     4021 cgttgattcc gatccagatc ggcagcagtt tttctatttc attagggcag attccattga
     4081 ctctttttgc gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca
     4141 tcctacattt tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt
     4201 acccgattgc ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag
     4261 gctatgtaga cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg
     4321 gcgcactttc cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg
     4381 cactatgggg gatgaattct tggctctttt ctttcgtaat gaatggtgcc agcgggttga
     4441 gtacagctat tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt
     4501 ttgtgccaga taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa
     4561 tcatgtccta gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg
     4621 gcagataaga aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga
     4681 ttaaagtagc gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat
     4741 cttcaatcac atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag
     4801 aaaaaatgat cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt
     4861 tttcaacagg tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga
     4921 tcgaacttaa tttactagca aaaatcctat aaaattcatc taaatagcga taataaatcc
     4981 gattcagctg atcattaaga tcgaagtata caaaattatt ctgtagttta taaaaaaacg
     5041 gcgaatgaag atgtgttttc atatgaccaa aatataataa ttctgaaatt tctaaaggtg
     5101 tcaattcttt cagcatcgtt aaatcagtaa agtcgatcca cttcaattca tcgaatccac
     5161 gcctgttacg agaagaggaa aagaaacgct gaatattttc tgtaccacgg attactttca
     5221 gccctgtatg ggattcatat tctccagcag aagcagatgg atcaagcaag agcaagtttt
     5281 gaggaaaatg cacgatactg tttgcaaagt cagtagcagt cagccctttt gttaaaacag
     5341 cattacttgt ggtatcaata tgtacataaa ctgactcagt catcatgttc ctcctcttat
     5401 ttcctcttaa tctc
//
LOCUS       DAXWLG010000159         5275 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXWLG010000159 DAXWLG010000000
VERSION     DAXWLG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.161"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..206
                     /locus_tag="ACGE4T_002336"
     CDS             <1..206
                     /locus_tag="ACGE4T_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8387549.1"
                     /translation="IKIFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKS
                     RQDGKVVYYSLANEDILSAIRVF"
     gene            complement(493..978)
                     /gene="vanZ-A"
                     /locus_tag="ACGE4T_002337"
     CDS             complement(493..978)
                     /gene="vanZ-A"
                     /locus_tag="ACGE4T_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8387550.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1131..2042)
                     /gene="vanY"
                     /locus_tag="ACGE4T_002338"
     CDS             complement(1131..2042)
                     /gene="vanY"
                     /locus_tag="ACGE4T_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8387551.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(2470..3078)
                     /locus_tag="ACGE4T_002339"
     CDS             complement(2470..3078)
                     /locus_tag="ACGE4T_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8387552.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(3084..4115)
                     /gene="vanA"
                     /locus_tag="ACGE4T_002340"
     CDS             complement(3084..4115)
                     /gene="vanA"
                     /locus_tag="ACGE4T_002340"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8387553.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(4108..5076)
                     /locus_tag="ACGE4T_002341"
     CDS             complement(4108..5076)
                     /locus_tag="ACGE4T_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8387554.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         1524 a         1167 c          906 g         1678 t
ORIGIN      
        1 gaattaaaat tttctatgct ttagaaacct ataaggaaat gtgcgtctgt gatttagcag
       61 aaatacttac tgctagtgtc gctacaacgt cacaccattt acgtttttta aaaaaacatg
      121 gaatggcaaa atcacgtcaa gatggaaaag tagtttatta ctctttagcg aatgaagaca
      181 tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat ctatctatga aatcttagtt
      241 tgggggtacc gtcaggaaaa tgcggattta caacgttaag gaaatttatt aaagagacca
      301 attcctcagc actaaaattg ataattgggc tttctttgtt actatagtgt taggtaagct
      361 ttttacttta tcaccggttt tcccctcact tcacacctac gggcgagttt ccccacatcc
      421 cgttttccat ctaatccaat tcattcaata tctttatgtg tatgaaagat cagattgctt
      481 gatttagaca tcttacacgt aatttattct taaatgggta cggtaaacga gcaataatac
      541 gagcaaaagt atacctacaa aaataataac tctgtctaat tttttttgat tcatatgctt
      601 attgcttaaa ccatataatt tcagtccaag aaagcctcca acagtatttg taattacatc
      661 tgttatgtct gtcgctccaa tagcgaagat aaattgaatt atttcaaaag taagacttaa
      721 aaccagtaca aaagcaaact taggtaaaaa tccgatttct ttaaaattga cattcaaaag
      781 caagccaaat ggaataaaga ttataacatt atctatcatc tctctgaaat tcccagtagc
      841 agtaaatgga gtcaagttaa gacttctttg atgataatta aatactgata aaatattgta
      901 ttgtaatttg aataacacta accagattag tgtcactaaa tataaagcta gcaatcctct
      961 agataatatt tttcccaata taatttcctc taattctttt gtttaagttg tatcattagt
     1021 atagcaatgt cctagttaac gtgggtttta aattaaaaag ctaacgttac cccaaaagtg
     1081 ctagtacaat aatgctgttc attaatttcg ttggtttcat tccgccatcc ttacctcctt
     1141 gaattagtat gtgttgatcc gggaaacact gtcacaatta caccgtctat attgtttcct
     1201 gatatctcat aacgaagatt agtcggcaca tgaatggtgg tatttttagt aacaggataa
     1261 taaaagatct catatttttc cccatttaca ctaacagaaa tggttttttc ttcttttagg
     1321 taatccatat attcctcgag aacgaaattc ttttctttca taatcgcact atgtggtaaa
     1381 ccaacatagc gaatatgcca tagttcatat tgaattcctg ttaactctgt tttgtcctct
     1441 ggataacgta aaatgaaccc gtatttccaa gcattttctt ctatccactt tccttcaggg
     1501 gctcgttcca ttttcgtcaa gcttgatcct acatctagtg ataaacctga attatgctca
     1561 ctataacctg ctggtaaggc atactcagcc cccatttctt ggtaaagcac actttgctca
     1621 tcaaagtctc gatagccact attaataata aaatgactaa cgccaccctt tacagcatca
     1681 ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca tataaatatt actatcaagc
     1741 aacccgtatc catttattaa ttcgtcatgt ttagataaat tcacgatatc tgacttcaca
     1801 ctttcttggc gaacaggata tttactattg attaatagca gatttccttg ataaacctgt
     1861 tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc cactattttc taaatgttct
     1921 ttgggatttt gatcataatt ttgaaattcg actttttcct gagaaaacag tgcttcatta
     1981 acgtagtcat aacctaagta tattaagaat aacaataaca ataaaaaaaa caacttcttc
     2041 attttcagtc tcctccttaa ctatatagtt ggaagtatga aatatttcta gaatcttccc
     2101 cttaaacggc acaatctcgt ttgagattgt gccgttttgc cccgtaaatt acagtcaatt
     2161 atttcgccct actggattta ctattatcac caatgtagat gaattttcat aagtgatacc
     2221 catgctgaat ttaacgaggc tgaactgcct gttgacaatg ggcggtgtga aataccaccc
     2281 ctctatgcga tcagtcttat tatcaggcta tcttgccgca gcgcacgcag ataatgaccg
     2341 caatatcgct ctgccttaca tcttcaatac tatctactat atataaattc cctaagaacc
     2401 agttacatac gtcgggtttc ctgccgaaag agttagctta tatagtttgt ccgtgcaacg
     2461 gttaaaagtt tatttaacgg ggaaatcaaa atagctattg gggtatggtt cgtctcttaa
     2521 tacatagtgc caccattcga ggctatatgc ttcaaaccca ctgttttcca tgatggagcg
     2581 caaacgtctg cgattttgcg cttcattgca tgatattcca tttgccgcat gatgagagcg
     2641 ttcatccata aaatcaaatc ggctccccat tggtacaagc tcacccgtgt ctaatcgata
     2701 aagcgtaaga tcaatggcac tgccgcggct atggcttgat tttgaagcca cgtatccttt
     2761 tgaaatcatc tcagttcggt caatattggg ataataactt tcctttgtca ggttattttc
     2821 cggctgtgca gcccattgca taaaacagtt tacagcacga ttaggacggt aaccgtccca
     2881 tagaagcaat ccgtaccctt gggtagcagc cagttctttt gccttcaaaa gcgattcagc
     2941 caactcgtat gtccctacaa tgcgatttac ttcataaccg tcaaccggtt ttccggtgaa
     3001 attatcccaa gtggcatatt tagcgtccca acgaacaccg tgtactattt catctaaaaa
     3061 agtaaatcct atttccatgc ttatcacccc tttaacgcta atacgatcaa gcggtcaatc
     3121 agttcgggaa gtgcaatacc tgcagcggcc atcatacggg gataacgact gtatgacgtg
     3181 aaaccgggca gagtattgac ttcgttcagt acaatgcggc cgttatcttg taaaaacata
     3241 tccacacggg ctagacctct acagccgagc gctttatata ttttttttgc cgtttcctgt
     3301 atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg ttataactgc gttttcagag
     3361 cctttttccg gctcgacttc ctgatgaata cgaaagattc cgtactgcag cctgatttgg
     3421 tccacctcgc caacagctaa cgcggcactg tttcccaata ccgcacaacc gacctcacag
     3481 cccgaaacag cctgctcaat taagattttg ctgtcatatt gtcttgccga ttcaattgcg
     3541 tagtccaatt cgtccgcgct attgactttt ttcacaccga aggatgagcc tgaacgcgcc
     3601 ggcttaacaa aaacaggata ggtaaacgta gctgccaccg gcctatcatc tttattaata
     3661 acccaaaagg cgggagtagc tatcccagca tttttcgcaa cgatgtatgt caacgatttg
     3721 tccatacaaa ttgctgagct ttgaatatcg cagcctacaa aagggatacc ggacaattca
     3781 aacagacctt gtatggatcc atcttcacct gacttgccat gcaaagctga aaatgctaca
     3841 tcaacatggt tgatttcata ttcatggttc tttttaacaa gtaatccgtg cattttttta
     3901 tccggcgaga gtacagctga atagcaattg tcgttttccc attccgcgca aggtttttcg
     3961 cacattttcc atacaccaga tttcgtaatt ccaatgtata acggctcgta tttttcttta
     4021 ttaatgttag cggctatctc tattgcagat tttaccgata cgtcatgctc ctctgagcaa
     4081 cccccaaaca gtattgcaac ttttattcta ttcatgctcc tgtctccttt caaaatccaa
     4141 acagttttta atggtttttt caacggtatc acgcaacgct tgctcggtat aataggccgt
     4201 atgcggtgtg attatcacgt taggcattct ttgaagttta agtaaaaatt gattatcaat
     4261 tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct ccttccaata catccaatgc
     4321 ggcaccgccc agtttcccgt tttctaatgc tttaaccaac tcataggtat ctacaagtgg
     4381 accgcgccca gtattgataa gaaatgctcc ttgcttcatt ctctgtattt gttcgtggct
     4441 gataatatag tgcgtatccg tattgagcgg cacatgaagc gtaacgatat cgctattttg
     4501 cagcaactca tcaaacggta catagtttac ctctatactt cggctgcgac tataagccaa
     4561 cactttacat ccaaatcctc gcagccgctc aataaccgct ttgcctatct ggcccgttcc
     4621 caccacacca actgtcatgt cgctgagtac cttgccacgg tcgctgtcca acctgaaatc
     4681 atgtttttcc acagagcgca caatcgattt tacgttgcgt actgccataa gaattagcat
     4741 catagtataa tcggcaacgc tatccggcga gtacgccaca ttgtcgacag tgatgcccat
     4801 tctcttagca gcagttgtat ctatatgatt gcagccgatg cttcgggtag aaatatattt
     4861 cacaccggct ctcttcagcg caagaagaat agaggcggaa atctctgatt tatgtcccac
     4921 actgatacat tgattgaaag gcgcggattt ggcgttggat tccgacacgt tggcgttaat
     4981 tatcgttgcc ataacgccaa agcgaggcga aagagcatgg aatgcatctg cctcatcctg
     5041 ctcacatcca taaacagtaa tgccgatgtt attcataatt aagaccaacc ctttctgtga
     5101 aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt caaatctttc tctgaagaac
     5161 gaaaacggct cgttcttacg gtattaagtc taccgtgtaa gaacgagccg tttcttaata
     5221 ttaaattgtt aatttcctaa gaaaaagtaa agataacctt gagattttcc taaaa
//
LOCUS       DAXWLG010000160         5273 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXWLG010000160 DAXWLG010000000
VERSION     DAXWLG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.162"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..246
                     /locus_tag="ACGE4T_002342"
     CDS             <1..246
                     /locus_tag="ACGE4T_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosX/FosE/FosI family fosfomycin resistance
                     thiol transferase"
                     /protein_id="HFF8387555.1"
                     /translation="VMQNSSNKLPKTYNHIAFKIDEQDIDSFVSKIQMLGLTVEPGRS
                     RVKGEASSIYFYDYDNHLFELHTGTLQERLKSYNSTT"
     gene            complement(518..808)
                     /locus_tag="ACGE4T_002343"
     CDS             complement(518..808)
                     /locus_tag="ACGE4T_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387556.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1210..1446
                     /locus_tag="ACGE4T_002344"
     CDS             1210..1446
                     /locus_tag="ACGE4T_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387557.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1453..3405
                     /locus_tag="ACGE4T_002345"
     CDS             1453..3405
                     /locus_tag="ACGE4T_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFF8387558.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3477..3974)
                     /gene="dfrG"
                     /locus_tag="ACGE4T_002346"
     CDS             complement(3477..3974)
                     /gene="dfrG"
                     /locus_tag="ACGE4T_002346"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8387559.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4301..4993)
                     /locus_tag="ACGE4T_002347"
     CDS             complement(4301..4993)
                     /locus_tag="ACGE4T_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8387560.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1711 a          915 c          865 g         1782 t
ORIGIN      
        1 gtaatgcaga attcgtcgaa taaactgcca aagacataca atcatattgc ttttaaaatt
       61 gacgaacaag atatagattc gtttgtctca aaaatccaaa tgttaggttt aactgtcgaa
      121 ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt acttttacga ctatgataat
      181 cacctcttcg agttacatac cggtactctc caggaacgtt taaaatccta caattccact
      241 acctgagact agtaatgact agtctctttt tatggcctct gatcccccca ttgagcgaat
      301 actaataggg cttcgaattg atctgttcaa tctttaagca atatccctca aatgagtttg
      361 gaaagtttag tgattcatca caaatactta aatacatgta gttatatata gattgaggtt
      421 cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc tgcatccatc tttccaaaac
      481 aagcggctag gacatttttg tctcagccgc ttgttttcta gtttattctt tcatcttgtc
      541 ggaccttttt tcttgggtat agacttttct catattctac cacaattttg ggtttcccta
      601 acacatgata ggatctcaca acaaattgct ttctattatt ataataacct gtaacgacta
      661 acttcatccc ttcgtctact tcagctagga agttcaaact atgggaagca atcaaacaat
      721 tgaccccatt taaactgaaa cggatcaacg gtctggcact aacttgcaaa gtacggatct
      781 tatctactgt tccatatttt ttctccataa ccttcacctc aataccatta tacgaacata
      841 cattcccttt gtaaagaaga aactctgttt tatctttttg actttttgtc tattaccttt
      901 cttaaaatca tattggcact tcctgcgaag atgaaggaca tgtactaaaa aaaagaggca
      961 gcagtataga tttaagtagt tcggttttgg agtacaaaaa gtgaaaatgt aaacctaaat
     1021 gagtatcaaa ttgcaactga atttgatact cgttttttat tgttaaatca acgtttgttg
     1081 ttaatttaca ccacaaaatg taagttaaaa tgtatataaa aatgcgaacg tgaaataatt
     1141 attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat ttactaagca aaaaggggtg
     1201 cattttccta tgcaatttct tgagccaaaa aataagaacg ctaagtctgt ggattgggaa
     1261 atatcagagc aagtaagggt gatagttaag caatacgctg aatatgcaga gcgtactgaa
     1321 agcgaagcag tggatgaatt cttattgaac attttagacg ataagaagtt tattgaatgg
     1381 attgccaaca agcgaagtaa taagcggatt gttgaaaaaa tgggtatcaa agatagagtg
     1441 ggttgataga caatgaccaa gcttaaaaga ttagccacga aggaagatga aattgtagat
     1501 gttaaaattc cgatttcaaa cgaagaatta aaagataggg ctaaacaata tgatttatta
     1561 actccaaaac gatttgcaac aaggtataac aaaatgttgt tcttgccaac gtcttttaaa
     1621 tggaacggtt ctgaatatcc cattcaatat aattactgca tcaatccgtt ttgttgtaat
     1681 tttgggaaag aacagcacaa attcaaagat gtaaaaggga aaccaagtcg atataaaatg
     1741 actggtagtt ccaaagataa aggtcataaa ggtatgtact gcaatgacaa tcctattggc
     1801 agaggtgtat cgcaaaactg tacagtaacc cctttatcta attggtctgt tgttgaagaa
     1861 attaaacgcc ttatagaaat caatagcatc caagatgttg agccagatta tcaatttcat
     1921 aaagaaggct gctcggaaga agaatcaacc ccattcaatg agccgaagca attttacaaa
     1981 agggggaaaa gtaggggcaa atctcaacgc tatcaatgta aggcgtgtaa gaagttcact
     2041 aatgttctac caaaaagaga agaaaccact acttatcacc aacaaaagaa caccattctt
     2101 ccaatgttcg ctaagatggt tgttggaagg gtttcagtca gtcgtacttg tgatatttta
     2161 ggaattggag ttggaaccta ctatcataag ctggagtggc tgtataggcg ttgtttggag
     2221 tttttagaac gatatgagac acaacccctt caaacaaaga aatttaacga gatgtggctt
     2281 aatacggaca agatgcacta ctacctaaat aacgtccgta agaaaggaca aggttcaaag
     2341 aaatatacgg ggtttgaaga ccttaatatg caaacgtata tagttgtatc tgctgaggtc
     2401 ttatcaagat atgtgttccg ttctgatgtt gcctatgatt ggaatatttc aatggatgaa
     2461 ttaaatgaag atacaagaaa attcaaagaa gaccatctca atacttttag tcgtaaaaat
     2521 gataggttag attggtctta ttatccacaa gaaccatccg ctaacgatag cgaaaatagg
     2581 aatgcttatc ttcacgaatt agggaaaatt actaatagga gtagatttgt cgatggttta
     2641 aatgttgatg ctccttatac tacaactgct cactattggt taattaaaca aatggttaat
     2701 gccgacgaat ggcgaatgat tagtgatgat gacttctcta ttagaaacgc cttttataga
     2761 gtattcacga aagaattaag gttgagcgat gcacatcatt ttatttgcca agttaacaag
     2821 acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag caaaagcaga actgctcgat
     2881 tggggagata tcagaggatt caaaacaaag tttctacgga ctttggcatc tcattatcta
     2941 actgaattac taacaagtca tcaattccac gaagaggcta ttagtaaaga tggagaaaga
     3001 tatcgcaaat atgccgataa ccctatcaaa catccattag ccactaagga taaaggattt
     3061 tattcagttg attgtagaac ggatttatct gctctggaac caaacgaaat cgcaaagatg
     3121 ctattaaatg ttaatgacca ttcgaccaat agctttattc aacagataag acgttatata
     3181 tcatctcttg aaagaccatt aacaacagct cgtggtgata agaagagcta tatttacgct
     3241 aacttcaatc cgaagtatgc tcaatttgcg attacaattc taagaactta ctacaacttt
     3301 tgtcgccctt ttaaatcagc ggataaaaag gttttgacac ctgctcaaag attagggatt
     3361 acagataaac aatttgattg gaaagatata atttatttca aatgacaaaa agagcttgga
     3421 gtaatcccaa gctcttgtgc tttatcttta ctgttcttct tcaattaacg cacccgttaa
     3481 ctcaataagt tttttctttc atatacatga aaatagtagt tatacggatt tttatcattc
     3541 tttatccctt tttgggcaaa tacctcattc cattcctcat aattcacttc tggaaaaaaa
     3601 gtatctcctt cgaattcatg atgtattttt gtgatgtaca ttttctcaac ataagggaaa
     3661 aacaaattat aaatctgttc tcctccgaaa ataaaaattt cttcttcgtt tttacataac
     3721 tcaaaaacat cttctattga atgaacaatt tcacaaccat taaaggtaaa ccccttatct
     3781 ctcgtcagaa taatatttct tctgtcaggt aaggctcttc cgattgattc aaggttcttc
     3841 ctacctaata ttatcggatg tccctttgta gtatttttaa catattccca gtccttggga
     3901 atcctccaag gaatgtcatt ctctttgcca atcactctat tcttatccat cgcagcaatc
     3961 aaagaaactt tcatttataa cacagccttt ttaactaatt taccatctaa tttcttattt
     4021 ctatcaaaaa accttttctc attatcctgc ccgttagttg aataagcaat tttttctata
     4081 agttatatta tcataatacc acaaaatcac aaattttcat tcaattatca ttttcaattg
     4141 cattttataa atcattttgt atgtaatttg gtatgtaagt taaaatgaaa aaagtagtcc
     4201 attttgaagt acattttgtc attttgtact ccaaaactga actacttagt atagattact
     4261 gttgcttctt tactaattta ggtttacttg ttaagaaaga ttatttacgc aaatgttctt
     4321 caacaatttg ctgagattta tcagctgtat cttccacttc atcttttgct ttttctaact
     4381 tttgaatata ttgtttcaag cttgagtttt cagcagttgc ttcgtctaat ttcttttgtg
     4441 tatctgttaa ctgagtttct acatttgttt tttcttgtgt cagtgttgcg atttgttgat
     4501 tcaaaccatt tacagtattt tgtaattctg aaagttttgc ttgtgcttct tgtagtcttg
     4561 ctgtccaatc tgcattcaga tgattgattt catcattttt ttggttgatc tcatctagtt
     4621 tgacattgat ttcattttgt ttgctttgta gttgtgcatc cttgctgact aattgacttt
     4681 ctaagttttg tttttctgct gccgattgat ttttttggtt ttctaattca tttacttttg
     4741 tttggtattc ttggacatct gcctttgaat cttgcaatgt ctgttctttt tgtgctagtc
     4801 gagcggtaag atctgtaatc gcttgctctt tatctgtcaa tgtatgatca agctgtgtca
     4861 gattctcatt gattgtatca atatctgtct ctccacccca caaaataatt tcatttgcgt
     4921 accgcgtgcc tgtgtagcca attagacata gaaatgcaac gacaatccca ccagtaataa
     4981 atttcttttt catttgcata gctccttaat ccaatacttt tacatgctaa aagtaagtga
     5041 tctcaaacac aatgtcaagc gtttttaaat tacatatgta attttaatat aaatactatc
     5101 aaacactcat ttaatcctcc ttttttatct atttaaaaaa tcttttttat agagaaaaat
     5161 gcaggttaat actttcttaa ataaatattt atggtgaaca aaaacaaaaa agcaagtttt
     5221 attaaacgta acccttttca tttcatagat tactgtattt attacaaaaa acg
//
LOCUS       DAXWLG010000161         5121 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXWLG010000161 DAXWLG010000000
VERSION     DAXWLG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.165"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     misc_feature    64..140
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            160..468
                     /gene="rplU"
                     /locus_tag="ACGE4T_002348"
     CDS             160..468
                     /gene="rplU"
                     /locus_tag="ACGE4T_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFF8387561.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            495..839
                     /locus_tag="ACGE4T_002349"
     CDS             495..839
                     /locus_tag="ACGE4T_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFF8387562.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            852..1145
                     /gene="rpmA"
                     /locus_tag="ACGE4T_002350"
     CDS             852..1145
                     /gene="rpmA"
                     /locus_tag="ACGE4T_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFF8387563.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            1579..4386
                     /locus_tag="ACGE4T_002351"
     CDS             1579..4386
                     /locus_tag="ACGE4T_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8387564.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            4577..5068
                     /locus_tag="ACGE4T_002352"
     CDS             4577..5068
                     /locus_tag="ACGE4T_002352"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8387565.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
BASE COUNT         1722 a          855 c         1106 g         1438 t
ORIGIN      
        1 ttttaaattt agttgactaa cggttgctgt tctggtaagc tagtactgtt gtaattgtgg
       61 actccacagc tacaaccgct cagaacaagt aataagcaca gcatatgctg tcacttcgga
      121 tggcgagtct aagtatatca gaaggaggtg ctttatagca tgtacgcaat tattaaaact
      181 ggtggtaaac aagtaaaagt tgaagtggga caagcaatct atgttgaaaa acttgacgtt
      241 gaagcaggag aaaaagttgt ttttgacgaa gttatcttag taggcggaga atctacaaaa
      301 gtaggcgctc caactgttgc aggtgcaact gttgaaggta ctgttgaaaa acacggcaaa
      361 caaaagaaag tcgttacttt caaatacaaa cctaaaaaac attcacaccg taaacaaggt
      421 catcgtcaac catatacaaa agtggttatc gacgcgatca acgcttaata gcgtttgaga
      481 aagaaggcct atatatgatc aaaggaacat ttaagcgaaa tgacgcaggt cagatcgttt
      541 cattcacttt gaccgggcat gctgatgctg gtccttatgg aagtgatatc gtttgtgcgg
      601 gtgtttctgc gttagcgatc agcactgtca acgggattgc ctcattagcc ggttttgagc
      661 cgattgttga aatgaatgaa gaagaaggcg gttatcttta taccgaagtc acttcaggaa
      721 tgacacaaga acaaaacaat attgctgaga ttctattaga aaatctttta ttaggattac
      781 aatcgataga agctgaaaac tcagaatata tccaaattaa aacaattaat gataaatagg
      841 aggtgcagac catgttgtta tctatgaatc tacaattatt tgcccacaaa aaaggtggcg
      901 gttctacatc taacggccgt gactcagaat caaaacgtct aggtgctaaa cgtgctgatg
      961 gacaaacagt tacaggtgga tcaattcttt accgtcaacg cggtacaaaa atctatccag
     1021 gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc aaaagttgat ggcgtagtac
     1081 gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt ttatccagta gctcaagaag
     1141 cttaattgaa tcttatgatt aaagaggttg tgacaagact tttgtcacag ccttttttgt
     1201 ttataaaaat aaaagttgta gtggaggcta cgaagtttac tttgtctttt tacacttaat
     1261 atttttttgc caatttggaa tcaggatgtt ttgataacta tcgttttcaa ttgatggaag
     1321 ctatattgtt tgctatcagt gaaataatta tatggtaaac agtgttcttt ttcttgtttc
     1381 cattataaaa atcgtatcgg gatttttcgt tcttttaaat tgaacatttc tatctatccg
     1441 cgtgttatca acaaaacaaa atcaggtaaa tagttgacga aacagaaata aatacatagt
     1501 attatgtttt gaagcctaag tacaatagtc tatagaaaag gagcgatacc attggcaaaa
     1561 cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg ctcatttaac aaaggcagaa
     1621 tatgatcaag gagtaacaac acaggaaatt gctgaagtgt tagggattca gcgaacaaat
     1681 agcagtaaag atctgaatca gctagtgcgc gaaggaaaac tgataaagac agacggacga
     1741 ccagtacgtt acatatatca aacactcgtt acgcacgtaa agccagttac aaaacatgta
     1801 gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata cactccaaat agatactaag
     1861 gatatctttg caaaaatcat tggagctaac ggcagcatga aaaattcagt ggaacaggca
     1921 aaagctgcga ttttgtatcc gccaagagga ttaaattgtt tgattacagg acctaccgga
     1981 tcaggaaaga cttattttgc acacgcgatg tatcattttg caaaagcgca tcatgtaata
     2041 gatgaggaaa cggaactgat tgttttcaat tgtgctgatt atgccaataa ccctgagctt
     2101 ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta caggcgcaga tgaagaaaaa
     2161 atggggatta tcgatcaagc ggatggcggt atgctgtttt tggatgagat tcaccggttg
     2221 ccgcctgaag gtcaagaaat gatcttttac tttatggatc atggaactta cagtcgtctt
     2281 ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg taggtgcgac cacagaagac
     2341 ccaggttctt ctctattaga aacttttgtt cggcgtatcc caatcaatat caaacttccg
     2401 gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag taaaaatcat gattgcccac
     2461 gaagcaaacc gcacgcagcg aaagatttct ttgacagaag atgtagcaaa agcattgatc
     2521 ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa atatccagtt agtatgtgca
     2581 cgaggatttc taaatcatat gcaatcaccc gaaatctcga tcacaatcga tgatttgaca
     2641 gaagggattc gtagcggatt gattcaatta gcaagcaacc gcagtgcaat ggcagagctt
     2701 tctaaaatat tggagccaaa gataactgta gcaccaaatg atacgatcat gaaaatccaa
     2761 tcggactcct atgagttacc ttataatcta tatgatatca tcggagataa agcagcttta
     2821 ctgaagtcgg atggtttgga tcaagaagca atcaatcatt tcatttctac agatatcaac
     2881 atccatctga agtctttcta taaagaccat ggtttttctt ttaatgcaga cagcaaatta
     2941 gcagaatttg tagatccgaa aatcatagaa gtgaccaatc agatttatgt aatggtcaaa
     3001 agatctttac catatgagtt tcagcaaaac tttatttatg ctatgagctt gcatatcagt
     3061 tcttttttga aaaaaatcaa cttaggagaa gaacggcata cgaatgataa catccgtgag
     3121 atggcttttg attttccaga tgaatatgct gttgcgaaag aagtgcgtag ttatatcgaa
     3181 acgtattttc aagtggagat tccagaaagt gaagactatt acttgaccgt tctactcgtc
     3241 tcgttacggg caaatcaagc aagcggcaga atcggtgtag tcgttgcagc tcacggaaac
     3301 agcacagcaa gcagtatggt ccaagttgtc caacagttgt tagatgcaga gaacgtacga
     3361 gcagtagaca tgcctttgga tatggatccg aaaacagcat tggaccgaat tgaacgcaat
     3421 gtacaagaag tagatgaagg aagcggggtc atactcttag tagatatggg gtcccttgcg
     3481 tcgtttaatt cgcaaattca aagagacact ggaattccag tacgtactgt tgatatggta
     3541 acaacttctc tagttttgga aactgtacga aaggtctcag tattagggac agatctagat
     3601 atgttgcatg aatcattgaa aaattttcga ggttacgcag aaatatcttc tgtagaatct
     3661 gttcaggaag caaaacctat tcataagaaa aaagctgttt tggctatctg cgcatctggc
     3721 gaaggtacag cacaaagaat caaagaattg atcgaacgag cggtaaacaa gcgtcaagaa
     3781 acagaattag aagttctagc tttgtcgatt gtggaagtga aagaagaact gccagttatc
     3841 caaaaagaat atcagatcat agctactact gggattactg atttgaatat ccaagcccca
     3901 tttattcctt tagaacgttt tattgatcaa aatatcgaag tcattttgga tcagttgctg
     3961 atggaatcag aattggagga aactgaattc atctccctag atgaagagtc agctaaaaat
     4021 acgtgtgttg agtttatctc tgataacttc atctttatta atggtagtaa gttaatcgat
     4081 ccaatgtggc aattttctac acaaatctct cagacgacgg gaatcggcga tgaagaatat
     4141 ggattcaaaa tcaacctagt gatgcacaca gcaggaatga tcgaacgtat tattcggaat
     4201 gaaccattga ctgtggaaga aaacgaattg acaaatacaa cgaatgatcc tctttatagc
     4261 caattggcag cctcagttgt tctgttggaa gatcagataa aagtaaaagt gccgatagaa
     4321 gaaatgtatt atctgctacg tctagtccat aatcagttag ataaaaaaga atacactgtg
     4381 ccatgaatac acaaaaacga tacactttta tagtgtatcg tttttgtgta ttggttaaga
     4441 atcctttaaa ttcaattagt gtatcgtaaa aaagttggca cgctttttgc ttatagtagt
     4501 gtgtgctagg aggtggttgt tcacacagaa atgatagggg tttctgtgaa gtagtgagga
     4561 agaaggagtt ttgcagatgg atattcgatt ggcacgtata gatgatcgtt taatccacgg
     4621 gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa cgtatcattg tgatcagcga
     4681 tgtagtcgct caagataaat tgagaaagtt tctactaaaa gaagctgcac cgccggggat
     4741 cacagcaaat gtaataaccg tgcagaaaat gattcaaatc tatcatagtg gattattatc
     4801 acgagcaaaa gtgatgcttt tattcaccaa tccccaagat gttgaagcgg ttgtccgctg
     4861 cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt tttactgaag gcaaatcaat
     4921 gattaccaat tttgtttcag taaatcaaca agatattgat tcctttcgtt acttgaacag
     4981 tcaagaagtg gagttagaga ttcgaaaagt acctgctgac aggaaaatcc ttttgatgga
     5041 actactagcg aaaatcaaaa aggtgtagca agaatggttg ttggaaaagg tcaaataatt
     5101 ctgttgtaat ggttagcaat t
//
LOCUS       DAXWLG010000162         5038 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXWLG010000162 DAXWLG010000000
VERSION     DAXWLG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.166"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(173..658)
                     /locus_tag="ACGE4T_002353"
     CDS             complement(173..658)
                     /locus_tag="ACGE4T_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFF8387566.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    748..1093
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1138..1779
                     /locus_tag="ACGE4T_002354"
     CDS             1138..1779
                     /locus_tag="ACGE4T_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8387567.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1798..2433
                     /locus_tag="ACGE4T_002355"
     CDS             1798..2433
                     /locus_tag="ACGE4T_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8387568.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2451..3266
                     /locus_tag="ACGE4T_002356"
     CDS             2451..3266
                     /locus_tag="ACGE4T_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8387569.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3585..5021
                     /locus_tag="ACGE4T_002357"
     CDS             3585..5021
                     /locus_tag="ACGE4T_002357"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8387570.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         1659 a          837 c         1100 g         1442 t
ORIGIN      
        1 tcgtagaatc aataaatatg gatttggcta actatggaag aatactcgta ttagttttca
       61 gcgttcatac tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg
      121 ctaaataaag aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat
      181 tttcataaac tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca
      241 taatcaacgt agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag
      301 cagcatcgat cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca
      361 ctgtacctgc tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc
      421 gcttttcttt taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga
      481 tgacaagata gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac
      541 caggtaggga aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa
      601 aagagatacc cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg
      661 cgcatcaccc tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa
      721 tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg
      781 tgtttttaga gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt
      841 ggagtcgatt ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc
      901 gttgctagat gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa
      961 ccacgtgatt aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg
     1021 caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata
     1081 atacgtcctc ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg
     1141 gagtatttga ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta
     1201 tttttattta ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct
     1261 ttgcatttta agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca
     1321 ccgctgttgt tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt
     1381 gatcgtttcg aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag
     1441 atctttcgtg gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg
     1501 caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt
     1561 ttaccttctg tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata
     1621 ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta
     1681 ctgcctttgc taggcgtggc aatcatctat cttacattga ctggtattct aacaatcgta
     1741 atgaaacaag ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg
     1801 attgaattga agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat
     1861 ctaatcattc cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc
     1921 acgcttttac gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac
     1981 ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc
     2041 caagacttcc agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa
     2101 atggtattga aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca
     2161 ttagggatta gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag
     2221 cgggtagcga ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca
     2281 acaagtgctt tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa
     2341 gaagatgggg taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt
     2401 gatcaattac taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa
     2461 ccagttttct attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa
     2521 aaacttcagc agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag
     2581 acgatacatt tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata
     2641 ttgatttagc caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg
     2701 actggtcaat gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct
     2761 attccaaaac gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg
     2821 atcaagtgct gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata
     2881 agagattagg tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag
     2941 tattaaaaga gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat
     3001 tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact
     3061 attatttgaa acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga
     3121 gtgaggattt tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg
     3181 aagccctaga tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg
     3241 gcgaagatgt tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag
     3301 tccctttttc cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg
     3361 caggctcaca acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa
     3421 tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc
     3481 gactttttca caacctcctt tacatatttt tattttgttg tggaaggggt ttcgtgttat
     3541 tctaaaagag gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac
     3601 ttacctcatt tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag
     3661 gagcttggca agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg
     3721 gaaagacatt taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag
     3781 aagatattgc tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga
     3841 cacggatttt tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga
     3901 gattgatcga cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg
     3961 atcttccaca agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag
     4021 atttcactca ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga
     4081 ttagtttgaa tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc
     4141 caggtgtgac agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg
     4201 catctgtcat caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg
     4261 cgtatacgcc aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg
     4321 cagaagacct gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg
     4381 ctgccatgag gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag
     4441 aattgctaga atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga
     4501 atgcattcaa tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag
     4561 gttcatcaga agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg
     4621 aacgaacaaa ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta
     4681 tcacaagccg ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca
     4741 agttgacaga agataaacaa atccatgacg actatcgaat caattactta caaagccacg
     4801 tcaaagccat caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt
     4861 cttatacaga tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg
     4921 ttgatcaaga cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact
     4981 ggtaccaaaa actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaaca
//
LOCUS       DAXWLG010000163         4989 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXWLG010000163 DAXWLG010000000
VERSION     DAXWLG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.167"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACGE4T_002358"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACGE4T_002358"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFF8387571.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACGE4T_002359"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACGE4T_002359"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFF8387572.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACGE4T_002360"
     CDS             2594..3379
                     /locus_tag="ACGE4T_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8387573.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACGE4T_002361"
     CDS             3392..4243
                     /locus_tag="ACGE4T_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8387574.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACGE4T_002362"
     CDS             complement(4367..4819)
                     /locus_tag="ACGE4T_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387575.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1688 a          815 c         1039 g         1447 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaa
//
LOCUS       DAXWLG010000164         4988 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXWLG010000164 DAXWLG010000000
VERSION     DAXWLG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.168"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            25..696
                     /locus_tag="ACGE4T_002363"
     CDS             25..696
                     /locus_tag="ACGE4T_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8387576.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACGE4T_002364"
     CDS             755..1195
                     /locus_tag="ACGE4T_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387577.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACGE4T_002365"
     CDS             1207..1875
                     /locus_tag="ACGE4T_002365"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8387578.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACGE4T_002366"
     CDS             1939..2562
                     /locus_tag="ACGE4T_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFF8387579.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACGE4T_002367"
     CDS             2618..3004
                     /locus_tag="ACGE4T_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFF8387580.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACGE4T_002368"
     CDS             3217..4608
                     /locus_tag="ACGE4T_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFF8387581.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1655 a          829 c         1063 g         1441 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggtt
//
LOCUS       DAXWLG010000165         4960 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXWLG010000165 DAXWLG010000000
VERSION     DAXWLG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.169"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            96..809
                     /gene="treR"
                     /locus_tag="ACGE4T_002369"
     CDS             96..809
                     /gene="treR"
                     /locus_tag="ACGE4T_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFF8387582.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            886..1332
                     /gene="msrB"
                     /locus_tag="ACGE4T_002370"
     CDS             886..1332
                     /gene="msrB"
                     /locus_tag="ACGE4T_002370"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFF8387583.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE4T_002371"
     CDS             1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE4T_002371"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFF8387584.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE4T_002372"
     CDS             2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE4T_002372"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFF8387585.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            3147..3725
                     /locus_tag="ACGE4T_002373"
     CDS             3147..3725
                     /locus_tag="ACGE4T_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8387586.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            3777..4259
                     /locus_tag="ACGE4T_002374"
     CDS             3777..4259
                     /locus_tag="ACGE4T_002374"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFF8387587.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            4376..4750
                     /locus_tag="ACGE4T_002375"
     CDS             4376..4750
                     /locus_tag="ACGE4T_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387588.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         1702 a          850 c         1082 g         1326 t
ORIGIN      
        1 ttaaaataag cttttcttaa aaataggaac attgaaatac aaaaagagct gtagtatagt
       61 gaagaaatga tggaagaacg agtgagggaa aaaacatgaa taagttcaat gaaatttttt
      121 tagatttgga acagaaaatt ttagcgaaag aatatcctcc gcattcgctt ttacccagtg
      181 agaaccaatt gatcaaaatt tatggtgttt cacgagaaac aattagaaaa gcgctgaatc
      241 ttttgactac ggaaggttat attcaaaaaa aacaaggaaa aggttcgatc gtactggatc
      301 ggaatcgctt cgactttcct atttctggat tgactagcta taaagagcta caggagaccc
      361 aacgtattcc aagtgaaacg atcatccata cgttagaaga aacagaagtg acaaaagcat
      421 tgaacgagat cactggctgg gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa
      481 aaatcaatgg cgaagtagtt atattagaca ccgattatct attaaaggaa atcgttccta
      541 ttttaactcc agcacaagca caaggatcga tctatgaata ttttgaaaat gagctttctt
      601 tgacgattgc ttacgcgcaa aaggagatca cagtagaaga agtaacggaa atcatgaaaa
      661 cagcgatgga tcttcacgat gacacttatg cagtagtagt cagaagcctc gttcatctgg
      721 aagatacccg ctgtttcgag tattctgagt cggttcaccg attagataaa ttccgtttcg
      781 ttgagtttgc ccgacgccga aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc
      841 tgtccacttt ggcgctatga taggatcaca aagggggcat ttttaatgga taaaccaata
      901 agaccagaaa aagaagaact gaaagaaaag ttatcaccca tggcttatgc agtcacacaa
      961 gaaaatgcaa cagaacgacc atttagcggg aaatatgatg atttttacga aaaggggatc
     1021 tatgtagata tcgtcagcgg tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt
     1081 tgtggctggc catcattcag caaaccaata acgaaaagac aagtgcgcga aaaagccgat
     1141 ttttctcatg gcatgcatcg aatagaagtt cgaagcaaag aagcagattc tcatctcgga
     1201 catgtgttta ccgatggacc agtagatcaa ggtggtctac gttactgcat caattctgcc
     1261 tcactgaaat tcattccata tgaaaagatg gatgaattag gttatgggga attcaaatca
     1321 cttgtagaat aatggaaata cacaaagagc ggtacaatac ggggatttac tcgatcgtcc
     1381 gctctttttt tattcaagcg attgctattt gaaagaaacg ctccccagac tccttttttc
     1441 acaaggtgaa atgtgctaca ataaagggaa cgtatgtgtg caaaagaaag aggactagaa
     1501 tatgtatgaa tatctgacag gtgtggttac ctttatcaac ccctattaca tagtagtaga
     1561 aaccaatggt atcggctacc agatcgcact aggaaatcct taccgctaca gtagcaaact
     1621 aaatcaaaaa ataaaaattt atgtccatca ggtaatacgt gaagatgcgc atcttctgta
     1681 tggatttgat tcgctagaag aaaagcaact gtttttgcga ttagtaagtg tttcaggaat
     1741 cggtccgaaa agtgcccttg caatcatggc aagcgaagac cactccggtt tgattcaagc
     1801 agtagaatcc agtgatgtca cttatttgac gaaatttcca ggagtaggga aaaagacagc
     1861 acaacaaatg atcttggatc taaaaggaaa attcggtgag ctgagtatcg atacaccttt
     1921 tagtctattt gatgaagcaa acacaaaaga cgctacagct ctatctgaag caatggaagc
     1981 tctctcggct cttggttaca gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga
     2041 agtagataat cagacaacgg atgagtatct acgccaagca ctcaagctaa tgatgaaaaa
     2101 ataaggagga gaccaaatga cagatgaata cttgttatca ccagaatctg gcgaaaatga
     2161 actgagtttg gaaaaatctc ttcgaccaca gctgctttct caatatatcg gacagagaaa
     2221 agtcaaacaa gaacttcaga tttacatcga agctgccaga aatcgggaag aacctttaga
     2281 tcatgttctg ttgtacgggc ctcctggatt aggaaaaacg acgatggcca tggtcattgc
     2341 taatgagatg accgtaaata ttcgtacgac gagtggccct gctatcgaac gtccgggtga
     2401 cttagtagca atcttgaatg aactggaagc aggggatgtc ctgtttatcg atgagatcca
     2461 tcgattacct agagtggtag aagagatgct ttattcagct atggaagatt tctatgtaga
     2521 tatcatggta ggacaagggc caaccgcaca tccggttcat ttccctcttc ctccctttac
     2581 tttgattggc gcgacgacac gggcaggtat gctttctgca cctttacgag atcgatttgg
     2641 catcatttct catatggaat attatgacga acaggatctg caggagatcg ttttacgttc
     2701 ttcggatatt tttcaaacag agatcattga agaaggggca ttggaaattg ctcgccgatc
     2761 acgaggaacg cctagaatcg ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt
     2821 ccaaggagac gggaaaatcg atcgtcctat agcggatcag gcacttactt tgctacaggt
     2881 cgatcaggct ggattggatt atgttgacca gaaattgttg cgaacaatga tcgagttata
     2941 tggcggcgga ccagtagggt taagtacgct ttcagtaaac ataggagaag agcgtgagac
     3001 ggtagaagat atgtatgaac cgtatctgat ccaaaaagga tttttaaagc gtacacctag
     3061 agggcgaatt gctactgctt atgcctatga acattttggt tacgactatc atatatgatt
     3121 tttgctttta gattggagga aaatgaatga gtgatacaac acgtacgaaa caattgatca
     3181 aaaacgtatt gattgatttg tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa
     3241 tcgcaaatga agcaaaaatc aatcgacaaa cattttatta tcattttaca gataaatacg
     3301 atttgcttcg ttgggtctat tgtgaggatg cattgcatta tttagatcca caatgtcttt
     3361 cacttgataa ttgggaagag caagcattga aagtgctgaa agcaattgct gagaagaaag
     3421 aattttatgc aaatactgtg ctttctgacc cagaaatttt acaaaaagag ttttctcaac
     3481 tgatccaaaa atcatttatc aagatttttg aacaaatgga tgaagaaaga caactttctg
     3541 caagagacaa actattttat gctcgttttt tttcttacgg ttgcagtggt gtattagtca
     3601 attggatcac gaatgaatat gaagaaacac cgatggaaat cgctatgcag cttttccgtt
     3661 tagccaagga tacggaatta tattcttatc gtctttatgc aaaagaagag ttggaagaac
     3721 agtaatagaa aaattttttg ttggaaaagc aataatcgct aaaaggagat ggggaaatga
     3781 caaaaatttt atttgtttgt ttgggaaata tttgccgttc accgatggca gaaggcttgc
     3841 tgagaaaacg aatagcaata gaaggaaggg aagcagaatt ttttgtggat tctgctgcta
     3901 caagcacgta tgaagttgga aagacaccgc accctggaac gaaaaaaata ttaaaccaag
     3961 aaaaagtaga tatgacgaat atggttgctc ggcaaatcac accccacgat tttgaaactt
     4021 ttgattggat cattggcatg gatcaagaaa atgtagaaga actgaaacga agagcaccac
     4081 gctcagcgca ggggaaaatc catttgtttt taagtagcat tcctggaaaa gaaaaagaaa
     4141 atgttcccga cccatactat accaataact ttgatgagac atatcacttg atcacagaag
     4201 gcctgaatca ttggtggaat atttggattg acgaaaataa aataacaaac aatttgtaaa
     4261 gttgacgctt tctttttaag gcgatgttag agcatttcct tgtttttttg taaaatgtta
     4321 ggtaaaataa aattaattaa gtaacacttt cttagctaaa ggaggagtgc attcgatgaa
     4381 cgaagagaca gtatcgccaa tgaagaagct tacgaagtta gttactcata cttcgtatca
     4441 actttttgat tcttttgtaa atgacactga agaacatgct aagaatgtac gattgcaagg
     4501 aaagtttatt cttgagctgc aaaaagctgc tactgaacat agtctagtta tcttacaagt
     4561 aagagcgaat cgtttgagta gcaagtatga aacgatctca ggctgggtgg cgacaaaaac
     4621 ggtcgaaaaa gaccgagtga tgatccgcct acaaaacgaa gagcagcaga tccggatggt
     4681 tcctgtagaa caaattgaaa aaataacaat cctttcacca gcaggggaac aggaacgatt
     4741 atcaaaataa tttataccag atagaaaagc acgtatgagc ttgtaccatc aatcattact
     4801 aatcaaaaat aaccgaatgc tttaagctga tatcagtaaa acggaacgtt ttaggatgag
     4861 tatcacagca tgacagcatt gggttgatta gttgaatcga ttggatggta caagtttttt
     4921 tgataaaaaa aggtcctgct agccggggag ctagcaggat
//
LOCUS       DAXWLG010000166         4947 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXWLG010000166 DAXWLG010000000
VERSION     DAXWLG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.170"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(409..3075)
                     /locus_tag="ACGE4T_002376"
     CDS             complement(409..3075)
                     /locus_tag="ACGE4T_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8387589.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3416..4867
                     /gene="cls"
                     /locus_tag="ACGE4T_002377"
     CDS             3416..4867
                     /gene="cls"
                     /locus_tag="ACGE4T_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8387590.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1444 a         1054 c          875 g         1574 t
ORIGIN      
        1 ttcataatat tcttcagcaa attcatccca ttctttttca ttcatttgat ttcctctaat
       61 tttttgttat atgttttcac ttttcggaat aaatcattct cctcttttat ctattctatc
      121 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
      181 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      241 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
      301 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
      361 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
      421 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
      481 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
      541 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
      601 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
      661 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
      721 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
      781 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
      841 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
      901 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
      961 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
     1021 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
     1081 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1141 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1201 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1261 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     1321 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     1381 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     1441 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     1501 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     1561 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     1621 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     1681 gcgttggaag acgatatctg gtccgccttt aacaaacaag taatttttgt tatcaattgt
     1741 gtgtccagta gacattaatt ttcgatctga gtcaaaagga agttctgctt gtcttgggta
     1801 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     1861 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     1921 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     1981 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     2041 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     2101 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2161 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2221 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     2281 gatacctaaa atcaataaag acaatgccaa aatagccaaa cttagttttt tactgaattt
     2341 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     2401 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     2461 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     2521 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     2581 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     2641 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     2701 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     2761 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     2821 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     2881 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     2941 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     3001 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     3061 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3121 aaaaagagac ctaagacaaa tacgcatttg tcttaggtct caccatttca tacaatacca
     3181 gaaaaccatt tggttaactt actgttgata ttatcgcaac aaacgttgcc ggttactccc
     3241 ctaaggagat attcaactgt actaatatcc taccttatta acctacaaaa agcaactact
     3301 ttagcccgat ttacctacat tttcatgaaa ggagtgaaaa gtatattcac cttggtaggc
     3361 ttctttcttc tatcatgcta tactaatgtt attgtatgta aggaggaaca tttttgtggt
     3421 atctagtatt ataaccgccc tttatctatt aaatgcactt attgctttgg tggctatttt
     3481 gatcaaaccc cgagatgtag cagccatttg ggcatggctc ttagtattgt ttgcccttcc
     3541 tggggtgggc tttgtattat atttattttt cggacgtgga ttaacggata agaaaaaatt
     3601 ctatctccga caaagtgact tgaaagaatt agaaaacttt cagtctttta gaggagatac
     3661 cattgaacat tacgatcctg acatgggcga taaagacaaa cagcaatttg ttgacttctt
     3721 ctcttcatta aatcgtatgc cgctgacaag aatgaattct gtcactcttc tcacagacgg
     3781 acaagagaaa ttggattcac tgcttcaaga tctaaaaaaa gccaaacatt cgatccatat
     3841 cgaatattac gcatttgtga cagataatat cggccagcaa gtcttacatg ttttagaaga
     3901 aaaagccgca gaaggcgtgg aagttcgaat attatatgat gcatttggct ctcatggcac
     3961 aaaagcaaaa gatttcaatc gtctaatcaa aaatggtgga catgtccata catttgttac
     4021 ctcacaaagg gcattacttc gtttccgatt gaattaccat gatcaccgaa aaatcgttgt
     4081 gatcgatgga aagattagtt ataccggtgg tttcaatatt gccaatcaat atgtaaatac
     4141 aacaaaaaaa ttcggctatt ggcgcgatac gcatatacgg attttcggtg ccgcttcttc
     4201 tttgctccag cttcgcttct taacagactg gaacgtctcg gtacctgaag aaaaaaaggt
     4261 cggctatcat ttgaattatt tctttaaaaa agcagatcga gatgaatcta agcttgctga
     4321 tacatccatc cagcttgttt caagcggacc gaataacgaa agggaacaaa tcaagctttc
     4381 atttatcaaa ttgattactt ctgctaaaaa acgtgtttgg atacagacac cttaccttgt
     4441 tcctgatgaa agtgtcattg ctgctttaaa aatcgcaact gcctctggtg tagatgtgaa
     4501 aatcatgatt cccaacaaac cggaccatcc ttttatttat cgagcaacac aatattatgc
     4561 tcggcagctg atcaaggaaa atgtacaaat ccttgtctat gagaacggct tcctccatgc
     4621 aaaaacattg ataatggatg atgaaatctg catggtaggt tcagcaaatc aagatattcg
     4681 tagctaccga ttgaattttg aaacaagtgc tgtcatttac gatcctgagt ttttagaaga
     4741 acttgctact cagttcaaag aagatgagac acattgttca tccatgacaa ctgaaacagt
     4801 caaggaaatg tctaactggc tattattcaa gcaacaaatt tctcgattat tttctccaat
     4861 cctataattt ttttattcaa gtagagaaaa atatactaag attaaaggga ctgtgacaaa
     4921 aatcttgtca cagtcccttc ttccgaa
//
LOCUS       DAXWLG010000167         4946 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXWLG010000167 DAXWLG010000000
VERSION     DAXWLG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.171"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..641
                     /locus_tag="ACGE4T_002378"
     CDS             <1..641
                     /locus_tag="ACGE4T_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8387591.1"
                     /translation="NLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKIW
                     YFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIPV
                     SLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNYT
                     LPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            657..1781
                     /locus_tag="ACGE4T_002379"
     CDS             657..1781
                     /locus_tag="ACGE4T_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387592.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            1759..2562
                     /locus_tag="ACGE4T_002380"
     CDS             1759..2562
                     /locus_tag="ACGE4T_002380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8387593.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            3022..3285
                     /locus_tag="ACGE4T_002381"
     CDS             3022..3285
                     /locus_tag="ACGE4T_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFF8387594.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            3275..3604
                     /locus_tag="ACGE4T_002382"
     CDS             3275..3604
                     /locus_tag="ACGE4T_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8387595.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(3783..>4946)
                     /locus_tag="ACGE4T_002383"
     CDS             complement(3783..>4946)
                     /locus_tag="ACGE4T_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8387596.1"
                     /translation="CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVTRLKKQ
                     RYTCKNCRTHWTAQSYFVRLSHSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTR
                     TLKEFKSYLPKPSKKILPRVLMVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLP
                     RLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELR
                     IREMNELRKAGQKSQAEKLKKNWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMI
                     DRLLEFSAPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYE
                     EGITSAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
BASE COUNT         1561 a          829 c          986 g         1570 t
ORIGIN      
        1 aaaatcttcc aattatcaaa attacgagaa atactttttt tattgctaca attataacgg
       61 ttgttaagtt aggcgtagct ttttttgcgg catatagttt tacttattgc cgaatcaaag
      121 ggaagaaaat ctggtacttt atcatcattg gtacgatgtt cattcctttt actgttgcga
      181 tgattcctaa ttatttgttg attgcaaaac ttaatttaat tgatactact tggggtgtga
      241 taataccaca atttgctgat gcgatgggca tttttttatt gacgcaaaca atgcgggaaa
      301 ttccagtgag ccttctagat gtggcggcat tggataacat caaacaaaca accgtcatga
      361 gtaaaattgt ttttcctctt actagacatg cgattacttc taccggtatt tggtttttta
      421 ttacctcttg gaatgagtat gtttggccgg tattaatttt acggacagta gataattata
      481 ctttgccgtt ggcgatgcag acctatatta gttctgaagg tgggacaaat tttaccgtag
      541 caatggcgat tagtttaatt acaatgtttg taccgttagt cttgtatgca atttttcaaa
      601 aatatattat cgggactttt gtttctgcag gtattaaata aattggagga caaatcatgg
      661 gagaaacgct tgaattaaga aatatcggaa aaaaatatgg agaacatacc attttaaaag
      721 aaattgattt agctgtggcc gctggagaac gaattgtgtt actgggacca tctggttcag
      781 gaaaatctac tttgctgaga atgattgctg gtttggaaaa aattacgagt ggagagcttt
      841 ttttaggtac acaactagca aataagctac cttgtggaga acgagagatc gcaatggttt
      901 ttcaaaatta tgctttatat cctcacatga ctgtaaagga gaatattatt tttgcattaa
      961 gggcgaataa aattaagaca gctgacattc agcaccgttt ggaagaagct ttgaagatgt
     1021 taggtttaac gccataccaa gatcgacttc ctaaagattt gtcaggaggg caacggcagc
     1081 gtgtagcatt ggctcgtgcg gtggttaaac ggagtaatta ttttttatta gatgaaccgt
     1141 tatcaaattt ggatgtgcgt ttacgattgg atgcacgaaa agaattggta aaaattcatc
     1201 aacgttataa acaaactttt gtctacgtca ctcatgatca aattgaagcg atgacgttag
     1261 ctgaccggat tgttttattg aatgaaggta gaattcagat gattgatacg ccgaaaaatg
     1321 tttatacgaa acctaaaaat acttttactg caaattttat tggttcgcca ggcatgaata
     1381 ttatgccagt tacttatgat caaggaacat taaaggtagc cagtcaattt ttaacattga
     1441 atccagcttg gcaaaaacaa cttcttcagg ttagtggctc attgtattta ggcataagac
     1501 cagaacacgt aaccttttca aaagaaacaa gcgatttaca cggtcaagtg aagtatcgtg
     1561 agctattagg tcaaagttat gcgtacaccg ttaaagtcga tggtatagaa atgattgtcc
     1621 ttaatgaaaa acctacttgg cagattggtg atgcagtcaa tctccgcata gaacctgaaa
     1681 gaatgcattt ttttgattat cagacgcaag aaaatttagg atatcctgat tattttattg
     1741 aagaaagagg gaatagccat gcgctttttg catctctctg atatccattt tagagaaaac
     1801 tatcaaacta acttgtttac tgctgaacta tttccaacaa ttaaaaatcc acagttggca
     1861 ttggaaaaat tgttacaaga actctcgtta acagtgtacg attttattct attaacaggt
     1921 gatttagtac atgaaggggg gatttctgat tatcaagtct tacggaaaat gttggatcaa
     1981 tatttcccaa ccacacctta ctatttttgt cgtgggaatc atgatcgtcg cagtgttttc
     2041 tttgaaggaa tgagtgttga aagcaatgaa acgggagagt atatccattg tacgaatcaa
     2101 aatgggttac gaattatttc cttggattct gctcaagata cccagcacga aggaaaaata
     2161 tcgataggac agatgattca attacaagag tggttgggta cacctgctga aaagggaacg
     2221 attttattgg ttcatcatcc attgtcgtgg gaagatccat ctattgtaac tgaagttccg
     2281 gaaggttttg ctgaattgat tgccgaaagt gatattttag gtatttttgt aggacatatc
     2341 catcaaggta gcagtgctga atttgctggt aaaaaacagt ttatggctga atctttatca
     2401 tttggtgtag atgaataccc agactattct ctttttacca atagaacagg attcactagt
     2461 ggcagtttaa ttgacggaaa aatttttact tatcagcatt atttaactcc acatcaaata
     2521 gaaatagggc gcatgaaaaa accaattact ggtaatcatt aaaaattatt gattaaaaat
     2581 aaagacttca ttcacttttt agccgtggtg ctcaatgtct ggttccactt gaagaacttg
     2641 gaaaatcgct atcaataaac cactatccct ccaattacaa aaagggtgga gaaagttttc
     2701 caagaaccat atcggtcttc gtggttgtgc cattcgtttt ttaccagagc ataccacatc
     2761 tttgtggagt tttcagacac gtcctagtta atttggttct tttaggagtc ttttcctgtt
     2821 ctgtgtgtcg ttgagtgttc gcttcaatta tcgctgtggc ggattttgaa acttttctat
     2881 ctatcaaggc catttagcac tatatccttt ccttatattt agataaatac actgcatttc
     2941 aaggagttca aaagtagttg tattaattcc atttgaactc tttccgtgat ataatgtgtt
     3001 tgtcagaaag aggtggagaa aatggctaag ttacaaattt ctatggacga aaaattgaaa
     3061 aatgaagcag aaagcattat tgatgaacta ggcatgagcc ccaaaacagc cgtcaccgtt
     3121 ttttataagc aaattgttga gcaaggaaaa atcccatttt ctattgaatt gtcggaaaaa
     3181 agtaaacgaa tccataatat tcaaagatta gcggaaaaat tgcctgtcca aaaactagac
     3241 actgacgaaa aaattgaaga atggttcaat gaagatgaat actaatgata tagcaaccgt
     3301 ctacgttgct ttcacagctg cagagggcgg taaacgccgg cctgtctata ttctaaaaaa
     3361 tggactggac acaatttcgt ttctgtcaat tacttcaaaa tatcatacta aatccgataa
     3421 aataaagctg aaatacgttg aaataaggga ttggaaacag gcaggactaa aaaaagaatc
     3481 ttggattgac gtaggaactc taaacgaatt acccaaaaac aacaaaaagg tcaaatggaa
     3541 aaaaattggg gaattaagtc caagcgattt acagcgactg gaagaacgca tggaagaatt
     3601 ataataatac taaaaagaag ggctctttgt caataaggac tgatgggttg tacaaaatca
     3661 aatctgggtc agaatgaacc tcattctggc tcagattttt ggctctttgt caataaggac
     3721 tgatgagttg tgcaaaatca aatccgggtc agaatgaacc acattctggt tcagattttt
     3781 tgttatctta cattgattaa ttgattgatc aaaaagattc taattctcat gttctcgaat
     3841 gatttaaatc cgtaggatac tcgtttcatt gtctttatgt gggtattctt cgcttcaatt
     3901 tttccattgg aataaggata gatcattgcg ctggtgatac cttcttcata ggtcagaagg
     3961 ttttgaagct tctcccgaaa gccgtcatct aacgtttcgg gaagttctgc caataaggag
     4021 aaaaataagt cagggtcttt gtctcgaaag gcttcgacta attcatgaaa aaagggatac
     4081 gcctccttta gaggcgcaga aaactcaagc aatcgatcaa tcatcattgc ttcagtgaga
     4141 aatgggtatt ttggtgctcg gaaacttttc catgttttgt actcataatg gttgatgctt
     4201 gcacgatttt ttagcagaaa gcgccagttc tttttcagtt tttctgcctg acttttctgt
     4261 cctgctttac gaagctcatt catttcacgg atacgcaact cattgagtgc ctgattcatg
     4321 tgtttgacaa tatgaaaccg atcaatcact actttcgcat ttggcagaac acgtttggta
     4381 agctggaagt aagcggcgtt catgtctgtg actaagaatt ctacttcttc tggattggtg
     4441 cagcctaaga aatagcttgt taatcgaggt aatttacgcg taggcaagac agcaattaag
     4501 tttcctgttt caccatctgc gcaaataaag ctcattttat cttctatgga agcgtgcgaa
     4561 cgaaattcat caaccatcaa tactctgggg agaatcttct tagaaggctt tggtaaatag
     4621 cttttaaact ctttcaatgt acgagtaacg gtagtcaaag atacctgaca acttttcgca
     4681 ataaaagaca aagatacttt ttcagtcagt aaagaagcaa ttttatatcg aacatgatct
     4741 gcgattgaat ggctgagtcg gacaaaataa ctttgagccg tccaatgggt acgacagttt
     4801 ttacaggtat agcgttgctt ttttaggcgc gtaaccaaag gcatatgatt gtattgttca
     4861 aaacggacaa tcgtttcctt cttcccattt ttcactataa ttgctttccc gtttccatct
     4921 accacagtag aaccacaact tctaca
//
LOCUS       DAXWLG010000168         4897 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXWLG010000168 DAXWLG010000000
VERSION     DAXWLG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.172"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..1886)
                     /locus_tag="ACGE4T_002384"
     CDS             complement(<1..1886)
                     /locus_tag="ACGE4T_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFF8387597.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPD"
     gene            complement(1899..3098)
                     /locus_tag="ACGE4T_002385"
     CDS             complement(1899..3098)
                     /locus_tag="ACGE4T_002385"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8387598.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(3088..4683)
                     /locus_tag="ACGE4T_002386"
     CDS             complement(3088..4683)
                     /locus_tag="ACGE4T_002386"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8387599.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1348 a         1019 c          814 g         1716 t
ORIGIN      
        1 aaatcaggat cctccatcgt tcgtccttca cgaatgagct gattgggaaa ctcttttgtt
       61 aaccaatcag gctctttcgt catctctttg atggcccgat aatacttttt ggccatgtca
      121 atggaactcg tggttaagat tgcagatttc gttggcctgc catttcgaaa atcaaatttt
      181 gtataggcat tatctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
      241 tcaaaaacag ctgggtccaa ataactttct tttttgacag gttccatttg atcaatcata
      301 cgattgatct gttctgatga atactctgca tatgtttcta cttcttttaa tttccgatag
      361 attttattct ctagtgaagt cggctccacg gtgttttcat gctctacttg aaatcctaag
      421 acagagccat cttctagcgc attcttaatg gtataggtat gcaagacctc tccatactga
      481 tcatgggttg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg aaaaatcggt
      541 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
      601 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg gcccattaat
      661 ttttggaagc gattggttcc ttttttctct tcttgtttct ttgcatattt caacgccgca
      721 tctagttttt gacgggttgt aatgattaca acatttgcat tggcatctgc caacagggtt
      781 tcacttaatt ctttggcact tcccgttcca acgatcaagc tattcgcttt ggcgttcccg
      841 gaagaaatcc cagtattaaa ttccgaagca aattttgtaa actctgtggt ggtttgatta
      901 tctaaatctt tgcggtccac taacatgatc gttcgatcga tccccgattt tcgtgctaaa
      961 agcttagtag atacaaaact agtcaacgtc tttcctgaac cagtcgcatg ccacacatac
     1021 cctgattgat gtttatttgc ggcaataaac aaggcttgaa tcgcatgtac ttgataagga
     1081 tgtaacacca tgagggtttt attatcttga tcttcactga cgattgtata atccgcaatc
     1141 aaacgatgag catccgggat atttaacact tgtttacaaa actcatataa gttttctact
     1201 ttttcattgt ctttcgttcg ccaactaaat aaaaacttag ggtgcaagtc tttcggtaag
     1261 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     1321 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaatgcctga
     1381 taaacaccat cttttgcagt tacttgtttc agctcgattt ggacaatcgg taatccattg
     1441 attaataagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     1501 atttgatgga ccacttcata agtagagatt cctccaccaa tgtcttgatt ggaataaagg
     1561 ataagtgaga cagaacccaa ttgcgaatct tcccgttcaa tcgtgatccg agccatccca
     1621 ttttcacctt ttaaccattt cgcagcatca aatggggttt tcgttcgcag cagtaattcc
     1681 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     1741 tttgagataa ttttttggcg taaattgacc caaaggtctt cttctgattt tagatccgga
     1801 cgatacgtcc actgattatg cccttctcca agtacacgaa tcaactgttc ttcgacttct
     1861 gcttcctcac ggtgactaat tttcattctt cttccctcct agacaaacat cttttgtaag
     1921 aagctttttt tcgtttcttt tagtaaatct aacttacgtt gatgaagagc gatcgtatca
     1981 tctagctgtt tgaagaaagt accgattttt tgttgttcat cctttacagg aaccttaaca
     2041 ctgatattct caatatcaga actgttataa gaaggcaatg cccctacttg ggagtatctt
     2101 ggcaaattta tagtttcaaa caatatttta ccaaaataga tatcccaagt attattaaat
     2161 aaataagcca tggtattgtt atctattaag aaagggcttg tgaccaaacg ttttctatta
     2221 agcattattg ctgcgccaac tttagcaaat ataatcgctg gcacatcttt tattggcttc
     2281 cattttttac gctcaatttg ctcattagat acataatgat ttgcatttct catttcatac
     2341 tcattaccaa tgttattcat gtcggaaact ttaaaaaatg gagtaccttc agaaccacct
     2401 tgctcggcat caggaaatcc aattcccgat tgagttttac ctaattctcc caacttacgc
     2461 tcttcccaat cttccgtaaa accaggaaac cggatttctg gcacttttgc tccgttttca
     2521 gggaacattt tttgtaagaa tccttttttc gtttccttca gcaaatctaa cttacgttga
     2581 tgaagagcga tagtgtcgtc gagttttttg aagaatatgc cgattttttg ttgttctttt
     2641 aaatcattgg gaatatagaa tactgtatct gaaacttttt tccagtctga acgaggcatt
     2701 tttgttccag atgtatcatt tgcagccttt tgatatctat cagcttgaat aagagaataa
     2761 ataaagtccg agtcgctctt cttagctcta aaaacccaga aatccccaat cgctacgcct
     2821 gtaaaagttg ccttgagcca attttttaaa tatggacgta actttccata tagaatatca
     2881 tttggtaaaa attcaatccc ttttctatta tcaaatttct ggctcacatc tttattcagt
     2941 ctaccttctc ctgccacgat atcttcaaat tcaacttttg gaagtctact ggagtttgta
     3001 cttttattaa gtctaacaac taaatcaatt aacttacgct cttcccaatc ttccgtaaat
     3061 cctgggaaac ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
     3121 ttgtaaccac atttcgtttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     3181 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     3241 atcaattggt tcttcttctt cgaaagtatc tacatagcgt gggatattta agttatagtc
     3301 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
     3361 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat agtttatttt gatttttccc
     3421 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
     3481 caacacaatc accgtcgttg gaatcgatgt cccaaagaac aagttcgcag gcataccgat
     3541 cacagcataa atactgccat cttctagtaa tttttgacgg atgacccctt ctgcagcgcc
     3601 acggaataaa actccgtgtg gtaagacgat ggccatggta cctgtttcct tcaaatgata
     3661 gaacccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccataacg
     3721 attaaaccga gaatcatcta agaaagtcgt atctgctgac caattagccg aataaggtgg
     3781 gttcattact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
     3841 atccccattg cggagattca tttcttcggc gtcgacacca tgtaaaatca agttcatttt
     3901 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctgggtg
     3961 tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa cccatcgttg gatcaaaaac
     4021 actaaagaat cgtcgttcct tttgatcgag cgttacgatt tgcgccatca tatcagaaac
     4081 catatgaggt gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     4141 cagaaactca tacgcatccc cgatcacgtc cccatcgtgt tctaaaacat ctacatcatt
     4201 caatttcttg atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     4261 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     4321 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
     4381 taagacgttg aaaagatact ctggtgcaat tgcatatccc aaaatatcaa cgattgtggc
     4441 aatcagatct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
     4501 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     4561 tcgatcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     4621 atccatttta ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     4681 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     4741 ccccctttta taaaaagcaa aaaaaaaaga aagaaactta ttcttgctaa taaactacgc
     4801 gaaaggagtc taacgttgtt tgatataact tagactgatc gttaacttat tttttatatt
     4861 tctacatagt tatctttaga accatttcag atacata
//
LOCUS       DAXWLG010000169         4790 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXWLG010000169 DAXWLG010000000
VERSION     DAXWLG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.173"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(201..1163)
                     /locus_tag="ACGE4T_002387"
     CDS             complement(201..1163)
                     /locus_tag="ACGE4T_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8387600.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1165..2604)
                     /locus_tag="ACGE4T_002388"
     CDS             complement(1165..2604)
                     /locus_tag="ACGE4T_002388"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFF8387601.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2789..4735
                     /locus_tag="ACGE4T_002389"
     CDS             2789..4735
                     /locus_tag="ACGE4T_002389"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFF8387602.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1527 a          818 c          790 g         1655 t
ORIGIN      
        1 atttcgtaaa acaaccgtaa cagttgtgta aaaatatcat tttagagcat gaaaaaagga
       61 gtggatttct ccactccaaa aaagtctaac ttttgggggt cactacaagt tgacaactcc
      121 tttttgttta caaatcaatt ggatatctac aaattgtatt ttctaacgaa ttttatagac
      181 attaaaaaat taatttaacg ttaaatactt cctccagaaa gtattgagat tggtaaaata
      241 taatccttat ttgtcttttc gcctttaatt tttttcagaa gaatatcaac aataagactt
      301 gcaatttcat caattggttg tcttattgtt gtcaattgtg ggaaaaattt ctcaataaaa
      361 gaggtaccat cgtatccaat aatttttaaa tcttctggga tattaagttt aagttgatga
      421 gcaattttca tagtgagaat ggctgttaaa tcatcagaaa caaaaatacc atctggtttg
      481 tgatttataa tagttgactt tatttccatt tcacgacgga tagtagacag attattagga
      541 attttgaaaa tttttccatt tggattttga aaagaaaaac ctagtgcacg taattctgtg
      601 ggagaatccg tgttatcatt tcctgtaatc ataatggtat tttgacaacc atttttttgt
      661 aaagtcttag ctgcaagttt tcccccttca aagttatctg aagaaacaat ggaaacatta
      721 ggagctaaat ttctatcaaa agcaacaata ggtgctctca cacgctcata atcagcaatt
      781 ccaagattat ggcttgatga gattatgcca tcaacttggt tggcttcaag catttcaatg
      841 tattctctct ctttagcagg atcattttga ctattgcaaa taatcgtttt atatccatgt
      901 ttaaataact ctatttcgag atactcaatg agttcagagt aaaagatatt actaatatta
      961 ggaaaaataa gaccaataag ctgaggtgac tttccttgaa gtccacgtgc taaattattt
     1021 ggtttataac caagagtttt catagcttca tgaacttttt gttttgtttt ttctgataaa
     1081 tatcccttat tattaatgac gcgtgaaacg gttgttgggc taactccagc taattcagcg
     1141 acatcagtta attttgctac cattttaata ttttaattca aagtattttc cagtaggttc
     1201 gcctgatttg atttgaatac cattttggtg attttcaggg aatacacgac tagtaaacac
     1261 tttttctcct ttattaataa aaatctcaat gattgagtta tctacaaaaa tgttagcagt
     1321 tgtttcttta gtatcaattg agcattcacg aatcgtacca aagtcagtgg catattgaat
     1381 gcctgctttg ctgcgatcta aaataatttt tccttctttg gtatctacaa tcaagctaag
     1441 tccatttcct ttactatcag agaaaagaag taactccgat ttctgatttg gaggaaaagt
     1501 tagttcaagc tcataagtat tatttgtttc tgcttttgct gtaaattgtt cagaactagc
     1561 acgtaatgat gtaatagctt ctactggata ttggtaaagt ttaccatctt ttattgaaag
     1621 ttcttttaca agactcattg ctccctgata atcatactta tcagttggat aatcgatatc
     1681 aggtagtcca atccaactga caatcagagc acgaccatca ggagtattga atccttgagt
     1741 cgcatacgct tcaaatccat aatcaagatt ttgaatttct gaagcgccca caagttttac
     1801 tttttctatg tcaagcgatt gacagatctt aaaagtattc ggatagatat tcccataatt
     1861 tagctcagat ttatccaacc cttgaggaca ataaatgaga acaggttttt tgtctacaaa
     1921 tactagattt ggacactcaa tcatgtattc tgaaccagtg ccgccaaaat caaggttgcc
     1981 aacttcctcc catgtttcca cgttattgtc atccgctttg taaagtttga taaagcctga
     2041 tttttctaaa ttttgtgctc caacaatcgc atacaattgt ccttcataat taaacatttg
     2101 tggatcacga aaatgatcag ttacatcagc aggtttatga ataagaacat tctcaccttt
     2161 cgtaatgttg tagtctttat ccatccatgc tccgatttga agtgtatcac gttcccaatt
     2221 ttcatttcga acgtttcctg tatagagtaa gaatagattc ccctctattt catatgcaga
     2281 accggaataa gcgccgtggc tatctcgttt tgtgtctgga agaagttctg ctcctgttac
     2341 atgaaaatga actaaatcat ctgattcatt gtgtacccat tctttcaacc catgagcagc
     2401 tccaaatggc caattttgat aaaaaagcgt atattttccg ttaaaaaaag aaaaaccatt
     2461 aggatcgttt aacaatcctg ttttaggttc gatatgatag ctagcacgcc aaggtgatcg
     2521 ttttacatta tccttaattt tatccatctc atctttagtc caatcagaat aagcacgata
     2581 tcgtatttct cgaggtaagt tcatatttta ctccttcaat ttatcttttt acaactagta
     2641 tggtaaacgt tttccgttga aaaatcaatc atttcattta aaaaaataaa ctatatgaca
     2701 aacgtttgac atatttttaa gatatgctaa aataaatttg catttcagtg aaccgttttc
     2761 acgtaaaaat aaaaaaggag tgattcgaat gaattacagt aaagtagctt ccgacgtaat
     2821 acaagcggtc ggaaaagata accttattgc agctgcacac tgcgctacac gtcttcgtct
     2881 cgtactaaaa gatgacacga aagtgaatca aaaagcttta gacgaaaatc cagatgtgaa
     2941 aggcacattt aaaattgatg gacaatatca agtgattatc ggtgcaggtg atgttaattt
     3001 cgtttatgac gaacttatca aaaaaactgg actttcggaa atttcaacag atgatttaaa
     3061 acaaattgct agtaaagata aaaaattcaa ccccattatg gcattaatta aattattatc
     3121 agatattttt gttccaatta tacctgccct tgttgcaggt ggtttattaa tggcattaca
     3181 aaacttttta acagctgctg gactttttgg accaaaatca attgaagaaa tgtatcctgc
     3241 gattgaaggt atttcctcaa tgatccaact gatgtccgct gcaccattca tgttcttacc
     3301 aattttagta ggtatttcag ctgctaaacg ttttggagca aaccaatttc ttggtgctgc
     3361 aattggtatg atcatgacaa caccagatct tggtggctca actgaatatt ggaatatttt
     3421 tggttatcat gtttcacaaa caaactatgc ttatcaagtt attccagttt tagcagctgt
     3481 ttggatatta tctatattag aaaaattctt tcataaaaaa ttgccatcat ctattgactt
     3541 tacattcacg ccattattat cagtaatgat tactggattt ttgactttca cagttattgg
     3601 ccctgtaatg ttattgttgt ctaatggaat tacagacatt attgtttggc tatataacac
     3661 tactggattt attggaatgg gtatttttgg aggaacatat tcacttatag tcatgactgg
     3721 tttacatcaa tcatttccag ctattgaaac tcaattactt tcagcgtgga caaatggtat
     3781 cggacatgga gactttatct ttgtagttgc atctatggca aacgttgctc aaggtgctgc
     3841 aacatttgcc atttggttct taactaaaaa ttctaagaca aaaagtcttg cttcctctgc
     3901 gggtgtctca gcacttcttg gaattacaga gcctgccttg ttcggagtta acttaaaata
     3961 ccgctttcca ttcttttgtg ctttaattgg ttcaggaatt gctacagcag tagcaggatt
     4021 acttaaagta gttgctgttt cacttggttc agcaggtttc ttgggatttc tttcaattaa
     4081 tgcaacatct attccatttt acgtattgtg tgaattgatt agctttgtag ttgcttttgc
     4141 aattacttac ttctacggta aaacaaaagc tgctggtatc tttaaagcag aagaaattgc
     4201 tgaacaaaca ccagttaatt cttcaaaagt agcaacaaca gatccaatat ctactgatgt
     4261 tgaatcaaca caaacaaaag tttcatcaga aacaattgtt agtcccattt ctggtaaagt
     4321 gattagtctt tcttcagtaa atgatccagt cttttcatca gaagctatgg gaaaagggat
     4381 tgccattaaa ccaaatggaa acacggtcca ttctccagta gatggtacta ttcaactcac
     4441 gtttgaaact ggacatgctt atggtattaa atcaaataat ggtgctgaag ttctaattca
     4501 tattggtatt gatactgtat caatgggagg aaaaggattt acacaaaaag ttactactaa
     4561 tcaaaaagtt agaaaaggcg atattcttgg agcatttgac cgttcaatga ttgagaaaga
     4621 aggtcttgat gatacaacaa tgattattgt gaccaatact tcggattatt cagaagtaaa
     4681 tatagtagct gaaaatacag tcactgaggg tactgattta cttgaaatca aataaatata
     4741 tttccctgag taaagatgga attttctttc caaaatcaaa taatatagat
//
LOCUS       DAXWLG010000170         4786 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXWLG010000170 DAXWLG010000000
VERSION     DAXWLG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.174"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..813
                     /locus_tag="ACGE4T_002390"
     CDS             <1..813
                     /locus_tag="ACGE4T_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFF8387603.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACGE4T_002391"
     CDS             786..1229
                     /locus_tag="ACGE4T_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387604.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1598..1726
                     /locus_tag="ACGE4T_002392"
     CDS             1598..1726
                     /locus_tag="ACGE4T_002392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387605.1"
                     /translation="MNTFSSERIRHDKAETVYFSFVMACADFFKSLGTRANIFIKL"
     gene            2401..3369
                     /locus_tag="ACGE4T_002393"
     CDS             2401..3369
                     /locus_tag="ACGE4T_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8387606.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            3379..3849
                     /locus_tag="ACGE4T_002394"
     CDS             3379..3849
                     /locus_tag="ACGE4T_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8387607.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            3865..4035
                     /locus_tag="ACGE4T_002395"
     CDS             3865..4035
                     /locus_tag="ACGE4T_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387608.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            4499..>4786
                     /locus_tag="ACGE4T_002396"
     CDS             4499..>4786
                     /locus_tag="ACGE4T_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFF8387609.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTV"
BASE COUNT         1824 a          772 c          792 g         1398 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcgatta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agttttgtag agagtgacct tataggcact ctcacattac gaacgatacg
     1501 tccctcggac gtactgaaag ggtgggactt atgagcgaac taatgcaaaa tttggctagc
     1561 aaaaaagcga aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aaacagttta tttcagcttt gtcatggcgt gtgccgactt
     1681 ttttaaaagt ctaggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaagg
     1741 cgtttcaaat gtctaagtat ataaatatat aagaatagag ttaagagtgc ttaaatcgaa
     1801 ttttagaggg aattcagaca attttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acagttttag caaaggagcc ggaggcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcaactata gggagtaaga
     2041 ttcccagttt agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctgta tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacaga
     2341 cattttgtat ttcataacgt ctgtatctct tgtgcgagta agaaaggagc tattttaaat
     2401 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
     2461 acaccagtcg aaatgaattt gttcttctct atcgtatcaa gaatgcgtga tgtaggtgat
     2521 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
     2581 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
     2641 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
     2701 atcgacggaa aagctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
     2761 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
     2821 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
     2881 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
     2941 acgatcgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
     3001 ttaacaatca aaaagaaata cggaaaaggc cgtgggaagc ctgtcattgg ctaccaattc
     3061 acatttaaac cagaaatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     3121 gaacaaaatc gggctttaga actagctaat cgacaaactc aacacgaaaa agaaactact
     3181 tcattacaag aaagaattaa ggaacaaaag cgaaaattag aacttttaca acagttagaa
     3241 gagttagaaa aacaacaaaa aaatagagaa aacgaaacgg ataaacaaaa gttagacaac
     3301 aattccgata ttcctctaga actaagaaaa gacttacttg ataatcttca aaacttattt
     3361 aaagactaag gagtattcat gacagataaa tcaataaaag aatttgcaga agaactaggt
     3421 gtcagcaaac aagccattag aaagtatttt gataaactgc caaccaagtt gacacctaag
     3481 ttgataggtg gaaaatacgt aatatcagca aaagctcaag agtttataaa aaatagtatc
     3541 aaccaaataa atgaagttga tactgaagtt gacagtttag ttgatagtat tttaattgca
     3601 gaattaaaag aaaaaaacaa acaaattgat catttacaag gggtcataga aatacaacaa
     3661 aaattactcg accagcaaca acaactcacc ttgcaggcaa atcaacaaat cgacaaacta
     3721 caaaaacggt tacaattagt ttatacggaa gaaacttcag aaaatgaatc aactactctt
     3781 tctgaagaaa cagaatcgat agaaaaacaa acaaagaaga agcaaaaatg gtggaaacta
     3841 tggaaataac tggaggaaac attcatgaca agaaaaaagc tcatatttgg ttctatttta
     3901 ctaggaattc taggcttcag tccgatgtta ttgtcagtga tcaataaagg aaataaacat
     3961 ggtgaattga aattgaatcc aaattattac aacagtccta acgaagagtt atttgtaaaa
     4021 gaaacggata aataataaaa aaagactgct ctaacgagca gcccaaaatg agattatcct
     4081 agtttagcaa cataaaccat ttattagatg aaaatgagat acaaacaaag cgtatcaaat
     4141 cctctcaaaa ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaattagata
     4201 tagataaccg cgaaaaatca agctttagaa agttgctttt agctagacca attaaaaatc
     4261 acaaagtgag cgtctctatc cattggtaga ggggctactt ggcatagtgt acattatgtt
     4321 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
     4381 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
     4441 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
     4501 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
     4561 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
     4621 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
     4681 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
     4741 agtattttca gacgctgata atacgattaa atatatttat accgtc
//
LOCUS       DAXWLG010000171         4753 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXWLG010000171 DAXWLG010000000
VERSION     DAXWLG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.175"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACGE4T_002397"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACGE4T_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFF8387610.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACGE4T_002398"
     CDS             complement(2064..3062)
                     /locus_tag="ACGE4T_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFF8387611.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACGE4T_002399"
     CDS             complement(3288..3941)
                     /locus_tag="ACGE4T_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFF8387612.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACGE4T_002400"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACGE4T_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFF8387613.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1393 a         1001 c          743 g         1616 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat aca
//
LOCUS       DAXWLG010000172         4728 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXWLG010000172 DAXWLG010000000
VERSION     DAXWLG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.176"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(54..716)
                     /gene="trmB"
                     /locus_tag="ACGE4T_002401"
     CDS             complement(54..716)
                     /gene="trmB"
                     /locus_tag="ACGE4T_002401"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFF8387614.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(786..1568)
                     /locus_tag="ACGE4T_002402"
     CDS             complement(786..1568)
                     /locus_tag="ACGE4T_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFF8387615.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(1772..2983)
                     /locus_tag="ACGE4T_002403"
     CDS             complement(1772..2983)
                     /locus_tag="ACGE4T_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8387616.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(2976..3713)
                     /locus_tag="ACGE4T_002404"
     CDS             complement(2976..3713)
                     /locus_tag="ACGE4T_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387617.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            3851..4285
                     /locus_tag="ACGE4T_002405"
     CDS             3851..4285
                     /locus_tag="ACGE4T_002405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFF8387618.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            4278..4622
                     /locus_tag="ACGE4T_002406"
     CDS             4278..4622
                     /locus_tag="ACGE4T_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8387619.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         1489 a          992 c          778 g         1469 t
ORIGIN      
        1 ttctgggatt tatttatgga tattaatgtt tttatgaaag cagaaagtta gtttcacaga
       61 cttttctttt tggtcttatc ttgaaattga gcttctactc gataaatacg ttgacctttt
      121 gaagaaaatt tttcttcata ctccgtcata atattgcctt caaattgact ttgatgtaaa
      181 tctaaccata cctgtttgag gatcatgcca tattgagaaa aacttgccag agaatactca
      241 aacaatcctt gattatctgt tttgaaatgg atttctccat ttggacgaag aatctgttca
      301 tctacagcaa gaaaagtctt atatgtcaat cgccgttttt catgacgttt ttttggccat
      361 ggatcggaaa aattcagata gatttgatct acttcgctgt cagcgaaata ctctgtcaac
      421 gctgatccat ccacatgtag aagtttcagg ttaggaagcg gttgttcgat caacttgtct
      481 agtgcaatcg aaacaacact gacctgcatt tctactccaa tataattgat ttcaggatgg
      541 gcttttgcca ttccagtgat gaactggcct ttccccatac caatctcaat gtggattgga
      601 tgatcgtttt caaacaattc attccatttt cccttccaca atgttggatc actaattaca
      661 aaattcgggt gagccgctaa catttccgct gctcccggac gatttcggac acgcattatt
      721 tatctccatt cctttcctct aattaaaaag gtctggccaa atctccgcag agtttgggcc
      781 aaacctcagc cactgaaaat tcgttttaat tgtaaaattt cttcgttcat ccgtcgatga
      841 tcttcacgtt tgcaatatct ggtgatttct tggagcaaat tcatggccgc ataccaatga
      901 aatttttcaa gcatttcgtc agtcgggcgt atgccataac tgatcagcca ctgattccag
      961 ctagacagtg gcacataatt tcctaaaatc gttgcaatat ccaccgcagg gtctgcaaac
     1021 atgattgaat cccaatcgac tagatataaa taatttttac atactaacca attacgatgg
     1081 tttacatctc cgtgtactac tgataagttt tccttatcta ttgacgggat ctgctcacga
     1141 agaaaacgat ggacaatttg cagaaattga tttttctgga gttcttccgg taattctttt
     1201 tcatattttt gcagcatctt ctccggcgtc atgacacgac caccgatacg ggtcagcatg
     1261 gttttaagag aatttgaatg atgtaaatgg tacaaaacat caatcacgtc gtttctttga
     1321 ccaatctcat taggagtcag caagcgacct tccagccact cttgtgctgt caaagtatcg
     1381 ccattccccg ttcgttttgt ccaaacaagt tttggtgtaa ttccttccct tgaaagagca
     1441 gccaacatcg gagtcgtgtt tcgtttaata aagactttgt cctcatcacg catgcctatg
     1501 tatgtttttc ctgtgtcgcc tttgatggga cgtaaccgcc actcttggtc taactgaaac
     1561 tccatcatgc attctcccgt cctcttactc tttgttttgc agtacttatt attctagtcg
     1621 cctgaacact acacgtcaag cacatatacg ttctttttca tttaattgcc attcaaaaaa
     1681 ccagcttttc tcaaaaaaaa tagaacgaac agcattctta ctgtcaacgc tctatttttc
     1741 attctcattt ttaccctaca cagctttgtt ttcagtctga catcttcttc aaacgagttg
     1801 gtacatatag tttaacaaag agcatagtaa caatcaaata agccgctagc ggaacccatc
     1861 gctgctgccc atctaatacg actgcagcaa tcaaaccaaa aataaccgac gcgataatta
     1921 atacccatgt gagtagatac tgaatcgcct gcttcttctg attcttatgg attggataaa
     1981 gctgaacgag aaccatatag cggaactggt tcgctaaagg taacagttgg aaaccgatca
     2041 aatataaaaa taagaaaccg ataatcagac taaaataaaa gtcgactact ccggcaatca
     2101 ataacccacc tataattact aatcggaaat aaagaccact aaaatcagaa cctcttgcca
     2161 atcttcttgc aaacaagtaa agataagtgt tttttgattc tcttttaata tgagtcaata
     2221 taggatccaa atagcgtctt cttttgatct ttgccgtaat ttcaggaaca tctgtgaata
     2281 aattgataaa ttgatagatc tgatgcaacc tatgttcttc tacatgcacc attttctccc
     2341 aatccaatcc tttttcttgt tgcttccaca aaaaccagta atatagactt gcctgtacta
     2401 atgcacctgc tattcctgcc catattagcc aataaaatga tagaaacaaa aagatcagag
     2461 aagaaaaaaa ccagcctaat ttccaataac gaataacttg ttgatctagt cgaaacaatt
     2521 ctgctctctt gatttgcaga tgactgaatt tcaaactcca taatatgatc agatagacga
     2581 aaaacatcga aaaggattgc tccgtactaa ccaccactaa tggcatggcc aatccgcatg
     2641 ccaatatcaa aaagatcaac ggaaaaatgc aagaatagcg aaaagctcgt tccaaatagg
     2701 tttgcatttc tttttcttta ggaagtaaaa aaacaatatc tgctgcctct gcaaaagtag
     2761 ccatcttacc cacgaataaa cagagcatcc aaaatacaga gattatgagc cctccaattg
     2821 caaacggtgg ctgcagggtt ttcaaccaat tagaataata taatcccaca ccgccaatca
     2881 agaaaagaca aactaaaaca aaatgatcgt tcaatacata acgcatatat ttcatcattt
     2941 tcttttgatg tcttgctaaa cgtttttgaa aaaaactact catgtgcctg ctcctttgtt
     3001 aaagcaatat agatttcgtc caaagaagaa tccggcagct gaaattcttt tctcaacatc
     3061 tcaagagaac cgtctgctcg tatctctccg ttatgtaaga ccacaaaacg atcacaatat
     3121 ttttctgcag tagctaaaat atgcgtagac atcaaaatac cggctccttc ttttttcatt
     3181 tctaccatca attcaagcaa tgcattgata gctagtggat ccaagcctaa aaatggttcg
     3241 tcgatgatat ataaagaagg ctcgatcaaa aaggcgcata agatcatcac tttctgtttc
     3301 attccctttg aaaaattagc tgggaaccag tcaagacgat tagataaccg aaaagtctcc
     3361 aacaactttt ctgctctttt cattgctata tccataggta tatcgtaggc catcgcagtg
     3421 acttcgatat gttctctcaa tgtcaattct tcatataaag aaggagtctc tggaatatac
     3481 ccaatttgtt tgcgatattc ttccgtattt ttctttaagg atagatgatt gatttctatc
     3541 gttccttttg aagcttccag cagaccgata atgtgcttga tggtcgtact tttccctgct
     3601 ccgttcagtc caatcaaccc tactatctct ccaggctgta catcgaaact gatatctttt
     3661 aacacaggga gatgtccgta cccccctgtg acatgatcga taattaaact cattcttttt
     3721 cctccgccat ttatttttat ttattatacc acgaagaaaa tgacggtatt ttgttttttt
     3781 ccttacaata gctttaactt atggtaaagt taagctgaag tatttattct ttatagaaag
     3841 gatgttttat atggaaaact gtattttttg caaaatcatc aatcaagaaa tcccaagcta
     3901 taaaatatat gaagacgaca aggtctatgc ttttttagac atctctcaag caacgaaagg
     3961 acacacatta gttgtgccta aacgtcatgt agcagatatt tttgaatatg atcccgaact
     4021 tgcaggagag gttttttcgc gtgtgcctaa gattgctcaa gcattggaaa aagcttttcc
     4081 agaaatgaaa ggtcttaata tcttgaataa caaccgtgaa ctagcctatc aatctgtttt
     4141 tcattcacat atccatttag tccctcgcta cagcaaagag gatgattttt ctattcactt
     4201 tgtcaatcat caagatagtt atggatcgga agaactaaaa gctattcaag aaacaatcgt
     4261 aaagcaggtg tcctgcgatg attaaaaact ttttcaaagg actgttattt ggagctgctg
     4321 ctggcactgt cggaggtctg ttattggcgc ccaggagcgg gaatgaaaca agaaagaaac
     4381 tgatcgaaga attggaagaa gcaacaactc tgacaaatga tttaaataac agcctgaatc
     4441 attttaaaag cgcattgatc gaaacaaaag aaacagctga atctgtgatt cctgctttcc
     4501 aagaagcaat caaaaaagat atcgaagatt ttaaattcca agccgaacca cgtatcacac
     4561 aaatcaatga acaagtagaa aaactaaaag ctgatctgcc agagtatcct gaacaaatat
     4621 aataatgatt attttctcaa cagatttaac tatataagtg agaaaagttt gcgtatttcc
     4681 taggaatgtg atgaaatttt gaattttgtt tgaaactacc acgacaac
//
LOCUS       DAXWLG010000173         4700 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXWLG010000173 DAXWLG010000000
VERSION     DAXWLG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.177"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(28..1176)
                     /gene="nagA"
                     /locus_tag="ACGE4T_002407"
     CDS             complement(28..1176)
                     /gene="nagA"
                     /locus_tag="ACGE4T_002407"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFF8387620.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1252..2088)
                     /locus_tag="ACGE4T_002408"
     CDS             complement(1252..2088)
                     /locus_tag="ACGE4T_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387621.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2272..3489)
                     /locus_tag="ACGE4T_002409"
     CDS             complement(2272..3489)
                     /locus_tag="ACGE4T_002409"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8387622.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3638..3799)
                     /locus_tag="ACGE4T_002410"
     CDS             complement(3638..3799)
                     /locus_tag="ACGE4T_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387623.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3872..3955
                     /locus_tag="ACGE4T_002411"
     tRNA            3872..3955
                     /locus_tag="ACGE4T_002411"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3906..3908,aa:Leu,seq:gag)
     gene            complement(4195..>4700)
                     /locus_tag="ACGE4T_002412"
     CDS             complement(4195..>4700)
                     /locus_tag="ACGE4T_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFF8387624.1"
                     /translation="RILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNL
                     FTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRL
                     IMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTF
                     IITVINR"
BASE COUNT         1409 a          988 c          763 g         1540 t
ORIGIN      
        1 ttagttttaa ggttaactta aattatatta tgcgtggaaa cgttccacgc catctagata
       61 agttgcttcc aatgtcatat cagggttcaa tacgataaag tctgcatcgc gtccttgagc
      121 gatttttcca catgtatcat cgattttaca gctgattgct ggaatcagac tagccatcat
      181 gactgcttgt tctggtgttg ctaatcccca gtctacaaca tttttaattg cttctttcaa
      241 tttcaatata cttccagcta agttgccaga ttctagacgt gctgttcctt cttttactac
      301 tactggaaat tcgcctaaat tgtaatttcc atcaggcatc ccaccggcca tcatacagtc
      361 tgtaattaat gcgacatgat catgtcctgc tttttccatt aggattcccg cagcatttgg
      421 atgtacatga tggccatcac aaatcaattc agaaaagaca tgttctaaag ataataacgc
      481 acccaccata cctggttcgc gatgattcaa tccacgcata ccattgaaag catggacaaa
      541 tacacttgcg cctgcttcaa cagctttttg cgcttcttct agtgtagcat tactgtgtcc
      601 tagagaaaca actactcctt cttcagtcac ttgtttgaca aactcttcta caccttgacg
      661 ttccggtgct aaagcaattt ttttgattaa accacctgaa gcttcttgcc attcatggaa
      721 agtatccaaa tcaggatccc cgaaataact tgggttttgt gcccctttgt gttcttctgt
      781 gaagaaagga ccttcaaaat agattccttt gattttagct cctggtgctt cctgatacat
      841 tttgccgatt gtctcagcta catcttttaa acgttctttg cttgacgtca atgttgttgg
      901 taaaaatgaa gtcactccac aagataacag accttcagac atcactttga tgccttctgc
      961 atcattatcc attacatcat gattcatata gccgtgaata tgtgtatcta ccaaaccagg
     1021 agcaatccat ttgccgcttt ggtcaatgat gttgacttct ccctcaggta gttctttggt
     1081 gtattcacca aaaagtccat cggtaatttc aagatagcct gcatgtttga cacctgacgc
     1141 taaaaagaat ttgtcagcat agataaaagt tctcattcta ttctctccct ttgtttttct
     1201 acctctaaag tactcttatt tagagaagaa gtcaagaatg gtctacacca tttacaaatt
     1261 ttttgaaatt tgtttcacag cttgtgctaa gccttcatag aatgctgctc tttctggttc
     1321 ttctttacgc ttgtatatta agcttttaag cacaaataaa tcattgaggt aatagtagct
     1381 attcttctct tgagcccaat agattccttg attcacctgt tctaacgctt cgtcgatttc
     1441 attttgatgg aataagaaat ttccataatt ataaaaaatt tgcgaaaatt cactttttga
     1501 tcgttcatca gctgattgat gcagcagttg gatgcttcga ctcaaatagt agcgtgcttc
     1561 agccgttttt cctgccacac catatatttt tccgatacaa ctaatcagtt gtatttctgt
     1621 gtcagaaaca tggattcttt ctttttggct agagtagctt aatgcttctt tcaaataata
     1681 aagggactga gagatatttt gacgcaacgt gaattcgcaa acaccgagat aataataata
     1741 gcgttgaaag tcagttgctt gatacagatt ttcagaaata tcagaagatt ttagtacttg
     1801 ttctaacttc cgataatcac gtttttggaa acaactatcc aacagttcaa ataatttatc
     1861 gtttgtccga gtagtttttc gagagagatt catcacatga tcaatcgtta cccctagccg
     1921 ttgacaaatc tgcatcatca ccaatacatt cggcagttcc tcattatttt cgatccgact
     1981 taatacactt tgtgaacaaa tatcatgcga aagcattttt tgcgtcattt tttgttcttt
     2041 tcgaatctct ttaatgatcg ttccaaagga ctcgattaac gtttccataa gtatcctccc
     2101 caaaatatgc gtattcgcat tctattcgta ttttacttta ggaagtcgca aacttcaaga
     2161 aaagacttgc aactaaataa tatactttag ttatttaaga aaactccact agaaagagag
     2221 cctcagtgtg tttgtctgtg gccctcctac tgaaaaattt ggtttttaaa atcatttttg
     2281 gcgataggtt gctaggaatt ctctcatttt cttagctgtt tgtttcaaat catccatttt
     2341 aggaagataa acgattcgaa aatgatctgg ctggttccaa ttaaatccac caccatgtac
     2401 aagtagaata tgatgttcat gtaaaaagtc caaaacgaat ttttcatcat ccaaaatatt
     2461 gaatcgcttt gtatcaattt ttgggaatat ataaaaagca gcttttggtt ttacggctga
     2521 aagaccagga atatcattga ttgcattata gatatattct ctttgttcat agatccttcc
     2581 gcctggcagg agcagctcat cgacactttg ataaccgcct agcgcggttt ggataatttg
     2641 ctgagacaat acattggaac acaaacgcat ggaggacaac atattcagcc cttctatata
     2701 gcctttgacg tgtgacttat ctccacttag gaccatccag cctactcgaa atcctgctac
     2761 acgatgagat ttggatagac cgttcagcgt aacaacgaac aaatcaggcg ctaatgtagc
     2821 aatcggaata tgttctagac catccatcac taagcgatca tagatttcgt cggaataaat
     2881 aatcaggtta ttttgtcgag ctacttcaac gatttcttct aataattctt ttggataaag
     2941 tgccccagta ggattattag gattaatgat aacaatagct tttgtccgac tcgttatttt
     3001 tgatttgata tcatcaatat caggatacca ttctgcctct tcatcgcaaa tataatgaac
     3061 aggtgtgcct ccagctaatg caatagaagc cgtccacaac ggatagtccg gcattggaac
     3121 cagtacttca tctccattgt ccagcaaccc ctgcatacac atcgtgatca attcgctcac
     3181 tccgttgccg gtgtagatat cattgatcgt cacattcgga aaatgtttca gttggcaata
     3241 ttgctcaatt gcttttctag cagaaaagat ccccttggaa tctgagtagc cttctgagtc
     3301 acgggcattc atgatcaaat cacgaatgac ttcgtttggt gcttcaaatc caaaaggtgc
     3361 gggatttccc gtgttcaatt ttaaaatact aatgccctct tcttgcatcc gctccgcttc
     3421 ttctagaacg ggaccgcgaa catcgtaact gacaccttct aatttgttgg atttttcaaa
     3481 ttctctcatc acttagccaa tccctcattt caaaaagtta gaactttcca aattttttgt
     3541 tatttactat ttcaatatca tactccatcc tagaaaattt tgccacttat ttaggaaaaa
     3601 cgtaagtatt aaaaaaacac aaacaagcgt tgagaactta attgtcatga tagtcattcc
     3661 atagattcgc tgcccaatcc attgcaattc cacaaatgat tcctagcata aaaccaagca
     3721 aaaaacgtcc gaatgcaagt ccaatcaata cacctaagat cattcccagt cctgaaaatt
     3781 ctccgtcata tttctccatc ttaataagtc ctcccatttt tgagcttgac aactatgtaa
     3841 aacctcgcta taattagtgt gttattatta cgcagatgtg ttggaattgg cagacaagca
     3901 tgattgaggg tcatgtgagc gttagctcgt gtgggttcga gtcccatcat ctgcactaca
     3961 actcgtgaag ttaattttgc ttcacgagtt tttttgtttg aacgtttctt tatgccatta
     4021 tatacatatc actgtatcct ttacaaaaag aacgtacaat catgtaaaca gcctgaggtg
     4081 agtatatgaa ggtcggaaac aagctactcg tagaaacttg caagtgttaa aacaattaat
     4141 cgaaaaacga gtaaaatccc tcacctaata ctaaagcgag ggattttttg tatattatct
     4201 gtttatcacc gttattataa aggtacaaat accaagcacg gttaatccaa attgcataaa
     4261 ttcatttttt tgtacactca tgcttttcat tagctcttta actgccatta gtactaagat
     4321 ggcgataaca acataaagta tatagtacgt ccgcattccg gaagcccata ctgtgggaga
     4381 aaatcccata attaaccttg aacaaaatcc cattatcatc aaaataacta aaaaaataga
     4441 agttttctta ttgtcaaaac ttaaataaat tccaacaatt aaacatagta ataatgccaa
     4501 gactactaaa gtagctatcc aagtacctgg ataaaaaagt gacagtttcg ttcctaactc
     4561 tgtaaagaga ttattcaggt tactactact ccaaatcatt gcccttttat ttccattgac
     4621 gtaagtaaag ctttgaccca ttgtattacc aaaataaata atcaggttta agaaaaacgg
     4681 taatgcagtc aatatacgca
//
LOCUS       DAXWLG010000174         4639 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXWLG010000174 DAXWLG010000000
VERSION     DAXWLG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.178"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..2655
                     /locus_tag="ACGE4T_002413"
     CDS             <1..2655
                     /locus_tag="ACGE4T_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFF8387625.1"
                     /translation="KSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSILASDA
                     FSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFPNQMMP
                     DALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMWLDAF
                     VYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLTNWSE
                     YKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLIMKNM
                     IGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFYLVPL
                     NLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAALFAVA
                     WGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSAEALV
                     NTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSANDSIN
                     YGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNISKNE
                     STFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLINTLS
                     GLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQAYLSL
                     FPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTKEVSF
                     MEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDKGWKA
                     KIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILYVWYL
                     DRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2746..3360)
                     /locus_tag="ACGE4T_002414"
     CDS             complement(2746..3360)
                     /locus_tag="ACGE4T_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8387626.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3478..3879
                     /locus_tag="ACGE4T_002415"
     CDS             3478..3879
                     /locus_tag="ACGE4T_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387627.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            4014..4352
                     /locus_tag="ACGE4T_002416"
     CDS             4014..4352
                     /locus_tag="ACGE4T_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFF8387628.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1430 a          803 c          866 g         1540 t
ORIGIN      
        1 aagtcattct ttagagaaaa cggacgctac gcagcagcta gtttcctgat tccgtttttg
       61 attttagcac ttgtttatct aagtatcggg atctatcctg gaagcagcag aagtattttg
      121 gcaagtgatg ccttttcaca gttttctaat tttcatgcca gcttccgaaa tgttcttctt
      181 ggaaagcaaa gtatttttta tacatggaat gcttctctag gattgaatta tttatcttta
      241 atttcttact atctaggcgg gatcttcaca ccactggtgt tcttttttcc aaaccagatg
      301 atgccagatg cactgtatgt tctgactttg cttaaggtcg gctgtgccgg attggctttt
      361 tggtttcttg cacgtacgtt caagattcct cgctgggggc atgttgcact tagcgtcagt
      421 tacgcattga tctcatttat cactgcacat tcggagatca tcatgtggct ggatgcattt
      481 gtttatcttc cattagtgat tttggggatt catcgtgtta tggatcttaa acggccgaaa
      541 cttttattta tcagttattt actcttgttt ttatccagtt tttatatggg ttttatgatt
      601 ggcgtatttt ctttcttata ttttatcgcc cgtctattga caaactggtc agaatataaa
      661 aaaacgattg ttccttatgg tatcacttct ttactggcag gcggcgcttc aatgatcatt
      721 gtattgccag cagtgctgga tcttcggtca aatggcgagg cactgagtca gatcactacg
      781 ttgaaaacag aagcgacttc ttatctggat ctaatcatga aaaatatgat tggtgtttat
      841 gacacgacaa aatacggatc gattccgttc gtttacgcag gtctgctacc attagtcctc
      901 tgtctgtttt attttgccag tcggaaagta ccgttgaaaa ataaattttt gtacggcagt
      961 cttttcgcca tacttatcgc aagtttctat cttgttccat tgaatttgtt ctggcatggt
     1021 atgcatgcac cgaatatgtt tttattccgt tatgcctatc tgttctcttt cttagtgctc
     1081 ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg atcgtgggct gttgttagga
     1141 atcattttgg tcgtagctgc tctctttgcg gtggcatggg gcatcaaacc tgaaggaagc
     1201 tacacctatg tgacgatgac ttcctttgta ttgacagtgg ttttcttagg gatttatgct
     1261 tttgctgtgg gattttatca gcataaaaaa ctttcattca atcatctgag tatattgtta
     1321 ttattgatca tgtctgctga agcactggtc aatacgaaca gtatgatcca tggcattctt
     1381 gatgattgga attatgcttc ccgcagttta tatactgatc cttatcctga cttgaaagct
     1441 cttgtaaatg aaacaaaaaa agattccgac tcgttttacc gcttggaaaa tctggatcca
     1501 gtatctgcaa acgattcgat caactatggt tacagtggta tcagtttgtt ttcttctata
     1561 agaaatcgta attcttcctc ttacctggat aaattaggtt tccgttcaag agggacaaat
     1621 ttgaatatcc gttataacaa taatactttg ctaatggatg ggtttacagg aatcaaatat
     1681 aacatttcta aaaacgaaag cacgttcagt aaatatggct tccgccaagc tggtcaaagt
     1741 ggcgactata cactgtatca gaatgtcaat gcattgccac tagcatttac tgcaccgttg
     1801 tccgttaatg atgttgagca gccagtgaca gataatttga ccagtcagac aaacttgatc
     1861 aatacattgt ctggtttaaa tgaacagtat tttacattct atacacctac tttgaagaat
     1921 cagttgaata cgacgatcac aaagacaacg actggcgtca cttactcaga aacagcagat
     1981 aaccagccaa aagagctcac ttgggaagtc catgtgcctg caaatacaca agcttactta
     2041 agtttgttcc caaccaattt tgcacaatta gaaagttcta gtgcgacgat cactgtcaat
     2101 ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat actatgatct tggctattat
     2161 cctcaagaga caacagtgac atttactgca agtttttatg gaacgaaaga agtaagtttc
     2221 atggagccta aagtcgttgg tctagatgtt gtagcctatc aagccgcaat gaatcagatc
     2281 aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag cttctgggac tgtaaatacc
     2341 gctgaagatt ccatgttggt gacaactatt ccttatgaca aaggatggaa agctaaaatt
     2401 gatgggaaac cagtcaaggt cagcaatttc aaagacgcat tcttgatgat aaaagttcca
     2461 gcaggtaaac atactgtcac tttcagttac ttgcctgaag gatttacgat aggtgcaatc
     2521 ctttttgtcc tatgtattgc attgtttatt ctttatgtct ggtaccttga ccgcaatcgc
     2581 cccttttatg gcacgattga tccagcaata gaagaaaaac cgcgaaatac acacagaaga
     2641 agacgaagaa aataaagaaa cgatggaatc tgtgtcactc ttctatttaa atggcaaaaa
     2701 tttcaatctt taaatgacaa aaaccacctg atttttggtg gttttttata tacctatgac
     2761 taaactatct cttgctgctt cgacaacttc tacagtgatc ttatccattt gattgattct
     2821 taaaatccga tcaatctgcg caaataaacg atgaataagc ctaaaatttc cttttgtgat
     2881 cttaatgata gttgtgatag cctcataatc agaaaaatct tctagcttga tatcaattcc
     2941 taaatctgcc catttgtatt ccaaaatatg atgtgtttcg tccttactaa ggttatcaaa
     3001 ttcatgtgca aatcctatgc gagagtaaag ctgcggataa cgagacaatc ttttttcaat
     3061 accaggcata ccgataagaa ccattgccag atcatttcta tcgtaaatat ctcttagctg
     3121 ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca ataattaagt caattgcttc
     3181 ataggtttta ggcacaggag gatcttcttg attgtatttt agtaccatat gcattgcttt
     3241 agtcagacct attctactag ttattgtgtt aatatccgaa ctgacacgag acatcttttc
     3301 agctggtaca gtataaaaaa ctttatttac acttaatatt tcatctgttg catttatcaa
     3361 gaacaagaaa aagtctgaat attcaaaaaa taacggtgta catttttaaa aggagatgta
     3421 taatcgagat ttagaaagcg ttttcctaaa aatttaatga attaggaagt gattactatg
     3481 aaaaaattat ttagcgcact ttttattctg ttatttggct ttattggtat tgggacttct
     3541 ggaaatatag ttaaagctag cacgatggtt gaacaaacaa gtgaaatggc aaaaactttt
     3601 agtcaattaa caccagaaga acaaagttac ttcaaatcca aaggatttag tgataaagat
     3661 actttttaca ctcaaaagat agagcctcaa gatccgaaaa cacgtttttc tgcaaatgta
     3721 ttgcggttaa ccgcatctac taaaaaaatt actaatacgc aggggcggac agaatatatt
     3781 ataacaagtc ccaattcggc gatgtatcgt atttctacac gcttgagttt aggtagtgca
     3841 aaatcatata ctcaaacagt aaatctagct aattcttaag gttatggtgg aggaatgtat
     3901 tttacattta ctgggaaaag acaatattac agtgtaaaat tagctatgca agtttataat
     3961 acatttggtg tagggtcagt ctcttgcact gcgggaggat tgacaatagg aaaatgataa
     4021 tttcttacta tgtcttaaat agaagtgatg atgaaggact cttcgttttt caatcattac
     4081 atgaggatac gatgctaaaa gagttttata atcggattaa gaataaacag atagaaacaa
     4141 atttatattt tttaagtgac tctatcttgc cttttattag caaccctaaa attcttaact
     4201 tgttgcagac aaaagatttg aaaagtttac cgattattgt cattgatgag gaaattgtaa
     4261 aatatggcgc ttatctaagc gtagaagaat tagaaaaatt aactaacatt ggaatgagta
     4321 ttcaggagga taatggggta catggaacat agaatgaata tgcacagttg agaacggaat
     4381 ttgacattta tttagaccaa cagggtatac cctgttggtc tttttgtgcg ttcatgatat
     4441 acttggtgtc atttttatat ttaaatggca caaatatttc taattatttg tcatttaacg
     4501 ctgaaaattt ttgctattta tctaaaaaag tgacaggata ctcatttggc cttcatagaa
     4561 ttttctaaat atgaatcatc aatgaattta attaaaaaaa taagtagggc aaaatataaa
     4621 tgtttttgcc ttacttgtt
//
LOCUS       DAXWLG010000175         4637 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXWLG010000175 DAXWLG010000000
VERSION     DAXWLG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.179"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            2..193
                     /locus_tag="ACGE4T_002417"
     CDS             2..193
                     /locus_tag="ACGE4T_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387629.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(567..1379)
                     /locus_tag="ACGE4T_002418"
     CDS             complement(567..1379)
                     /locus_tag="ACGE4T_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387630.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1542..1649
                     /locus_tag="ACGE4T_002419"
     CDS             1542..1649
                     /locus_tag="ACGE4T_002419"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387631.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1707..2774
                     /locus_tag="ACGE4T_002420"
     CDS             1707..2774
                     /locus_tag="ACGE4T_002420"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8387632.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            2916..3119
                     /locus_tag="ACGE4T_002421"
     CDS             2916..3119
                     /locus_tag="ACGE4T_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFF8387633.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3179..4381
                     /locus_tag="ACGE4T_002422"
     CDS             3179..4381
                     /locus_tag="ACGE4T_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8387634.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1549 a          740 c          969 g         1379 t
ORIGIN      
        1 tttgaaacaa aaatttttca ttgcaataat tgcaacaaag aaatttcatc gggggaagag
       61 gtttggacaa aaatggaaat ttccgccaaa agtaactgct cctcaactga aatcaattaa
      121 agaactagaa tttgagaatg cactggtaat ttgtatgaca tgtactgatg aagtatttcg
      181 aaaacatttt taaaggttga gatgtatata gatttaacca atcatttatc tctctcggaa
      241 aaggaatatt aactaaccag cgggttatca aaaataggta ggaaaactgt acttaatggc
      301 ttatttcgcg ctgattatga gaatatcagt ctgtttgatt aatttttagg gttcaaaata
      361 cagagacaaa ctccaggtcg ggtttcgcca caagcgaatt acccgatctg gagttttgtc
      421 tattgcagga aggcgtagcc ggactgcagg aataaagccc ccggttctaa cagggggcta
      481 acaaagaaca ggaaactgtg acaactaccc attggggagt gtcctagcag tattttcttt
      541 gtcacgtgct tatcaatttt gtcacctcat tttgatgatg gatacgctgc atttaacagg
      601 cgttctttct cgacttccat cacgaatggt agataaagat attgctgttc tagtagatat
      661 tgattgtcct ctgtgtactg ataagaagcg gtttcttctg aatagaccaa cggggcaatt
      721 cgcacatcgc tggtcaattg attaattgag tatagaagga tggctttctc acctagttcc
      781 ttgggtaaat gtttcccaaa ataggaatag gatttttgat ttgctaaata gacgagtatg
      841 cgatccacac taaagttgga gaaggtaaag ggcatcatgt gcagaatatg attttgatta
      901 tcatgttgca aatagagctg ggcttcctta ccaaaattag ttgaaccaag ataaagctgc
      961 tttttttcat cgatacattg gaaagtaaac tggtcttgtt cagttggtgg aatgagattt
     1021 gtaataaaaa attcaatcat tcgatccatt tctttttcac taacaaggtc agcgtgtttg
     1081 atttcgtcta ctatgagacc ttcaatctta ggatcaagct tacctgcctt tttttggagc
     1141 atttctaaaa tgtattgatt ctcttgatca ccatacaaaa gatattcatt ggtgacatga
     1201 cctatctctg cgatttggtg aagggtttcg gtttttggaa ggttgatccc acgttcccag
     1261 ttattgactg tacttcgcgg taaatcacca attagtttgc caaatttctc catactcaac
     1321 tttaattctt ggcgaatctg ccgaattcga ttgccgactg cttgcttgtc tatctccatg
     1381 aaaactcctc ctcaggataa gttcttaaat ttagtatacg tgtgtactta aaaaaagtca
     1441 aaacatattg acttaaaata aatacaggcg tacaataaaa atgtacttaa aataagttca
     1501 ataggttctt tgacaagtaa atagcagaag gaggtttgtc gatgaaccaa gttaatcaac
     1561 atgatctcgg tgaagccatt cgagtttctc gagaagaaag ggggtggaca caacgatatt
     1621 tagcagaaaa agtggggatt tcgagatagt tattatcgaa agtagaaaaa ggaacgagac
     1681 aattaagtga agaaaaattg aatcttgtgt tggatagttt gcaagaagag gtcgttccag
     1741 ttaaccgtgt tttgattgat tatctaacca ttcacttttt ctcgaatcaa catttaaagc
     1801 taattgaaga gattattgaa atgcccattg aacgttttga ggaactggat tatgcgccaa
     1861 agggctatat tggccaatac gtgtggaatc aagtaatcac gattcggtat tccattgacg
     1921 atgcggttaa aggaactgtg atggagtttt caggtcaagg ctgtaaacac ttagcaagat
     1981 ggctaaaaac acgaaaaaca aattggcaac agttttttcg taaggtatta gattatcagg
     2041 ggaactttac acggattgat tttactttag atgattttgt ggggagtctc agcattccag
     2101 aacttaagca taaggtaata ctaggccatg tgtggaccac ttttcaagtg agtgaatctc
     2161 atggcggtac ggatatcata aacaatgaat caaatggtga gaccttgtat ttaggctcaa
     2221 agaagagtca gtgtcgtttt tgcttttatc agaaggacca tgaacaacgg aagcgacgag
     2281 gaattccttt agaagaggca aaagtaaaga atcgctttga actacggtat cgaaaagaga
     2341 aagcgcaaag cttggcaaag attatttcaa aaacccatga tttaaccaaa ctgttctttg
     2401 agttacttaa tggagcaatt tgtttttatg accgtgatcc aaatgatcta ggtgcaaaag
     2461 ttgatgccaa atgggcagcc tttattagca attatggcgc aatcaccatt tctttagata
     2521 caattcctca aagttttgag aaaactatga gttggttaat tcatggtgtt tcaccatcat
     2581 tagtttttat tgcacaggtc gatcaaacgt ttcattcttc cttgttgcct ttgattattg
     2641 aacaaggaga aatgaatcca agacagatca aacttctcca aaatatgaag gcagacccgg
     2701 agtattatca ggaggaggtt gagtattatg aacgaaaatt acaaagtata tcaaatgaga
     2761 aaacaagcta ctaaaaaagc aaagtactgc ttacacaaca agttgtgcaa cgtactccgc
     2821 ctcgacaaga cgagtataac acaaccatcg aatgagaaac aatagattat aaagaggatt
     2881 attttaaaag gagtaaagaa tcaggaggtg agagaatgga aacaaaatgt gatttcatgg
     2941 taaatcgtgc aattttgata gagatgggct ttaaacctag tcaagctgca cgaatgatta
     3001 aagagagcaa ggcatatctt gcaaggattg aaggtattga tttttataat aatcgccaag
     3061 ttggtgtggt gccatcccga gtaattgaac atctttttca cattcaagtt gcggaataaa
     3121 cagtacaagc gcggtactat taaagtagct tttctcaagt acagggagga atggaacgat
     3181 gccaaaaatt aaaaatattt accaagataa aaagacaaaa aaatggttct tccgagcgta
     3241 tctaggtata gatgaaaatg gaagaaaggt tcagaagact aaacgaggtt atgcttcaca
     3301 aaaagatgcc aaaattgctt atgacaagta catggaaaca catgatttta acaagacaat
     3361 tcctaatcaa ctgtgttcta ccaatcaaat gactttcgaa gagttttaca aggataggtt
     3421 tgtaaaatgg tacgagagac aggtcaaaag tcaaacatat gagaatgcgc aattcatttt
     3481 cgaaaagcgt atgcagtttt tctatcattt tcgtgttcga gatattacaa gtcacgatat
     3541 tgaggattgg ttatttgaac taagccagac agagtcacgt aattcacgga aaaaagttaa
     3601 tggcgaaacg ttatcaaaat cttatatcaa tcggattcga gggcacctaa aaattgtatt
     3661 ggatcgggct gttaaagagg gggtgattgc taaaaatccc gttgaagatg tttcttcgct
     3721 tccttctgag aataagaaag ttgagttttg ggaacacagt gagtttcaaa aagtgatgaa
     3781 tgaattgaag gatgataaga ttcaaaccca ccatcggaag attgtgtatg aaatgctctt
     3841 ttatactggc attcggattg gagaacttga agctttgtct tggtcgaatg tagattttga
     3901 gaaaaacacg ataacgattg agaagacatt gatttataat acaaaagatg attggtactt
     3961 ttcgactccc aaaactaaat gtgcttatcg aacgattggg ttggggaaga agctttcctt
     4021 aaaactcaga cagtggtatg acttgcaaaa acgtattgga aattttgaat atgttgctca
     4081 gttggacgga acatttacgc caccttattc ttttgcgaac tggttaaaag aagctgctaa
     4141 aaaagcgggt gtcaaaccga ttaagcttca cgcgctacga cattctcatg ttgcatttct
     4201 aattgagcag aatattcaac cgctatctat tcaagaacga ttgggccatg cgaatattca
     4261 aatcacttta ggaacttacg gtcacttata tgcaaagtca gatcaacaag tggtaagtgc
     4321 gattgataat gttcaagagg gcgctattct ttcaaaaaat gacaatttat taccagtctg
     4381 agttttccac ggttctacca taagacaaat aaatgctttt gcaccagtgt tttcagtcga
     4441 attgctcctg ttcaagatgt atcggaaata gcgagaaaat aagcaaattt gcataactat
     4501 aataaaaagg acttagtttt gatgggaaat ttttcaaaat taagtccttt tttatgtggt
     4561 tgaaacgggt atgatcattt taaggaagtg atctaagatg aaaggacgac tgaacaatta
     4621 tcatattaga aaagctt
//
LOCUS       DAXWLG010000176         4620 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXWLG010000176 DAXWLG010000000
VERSION     DAXWLG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.180"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(52..522)
                     /locus_tag="ACGE4T_002423"
     CDS             complement(52..522)
                     /locus_tag="ACGE4T_002423"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFF8387635.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACGE4T_002424"
     CDS             complement(681..1139)
                     /locus_tag="ACGE4T_002424"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFF8387636.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACGE4T_002425"
     CDS             complement(1310..2455)
                     /locus_tag="ACGE4T_002425"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFF8387637.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACGE4T_002426"
     CDS             complement(2468..3385)
                     /locus_tag="ACGE4T_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFF8387638.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACGE4T_002427"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACGE4T_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFF8387639.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1446 a          844 c          873 g         1457 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
//
LOCUS       DAXWLG010000177         4607 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXWLG010000177 DAXWLG010000000
VERSION     DAXWLG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.181"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(105..629)
                     /locus_tag="ACGE4T_002428"
     CDS             complement(105..629)
                     /locus_tag="ACGE4T_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFF8387640.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACGE4T_002429"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACGE4T_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFF8387641.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACGE4T_002430"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACGE4T_002430"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFF8387642.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACGE4T_002431"
     CDS             complement(2351..3031)
                     /locus_tag="ACGE4T_002431"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8387643.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACGE4T_002432"
     CDS             3157..4026
                     /locus_tag="ACGE4T_002432"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8387644.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACGE4T_002433"
     CDS             4052..4567
                     /locus_tag="ACGE4T_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFF8387645.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
BASE COUNT         1308 a          984 c          851 g         1464 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtac
//
LOCUS       DAXWLG010000178         4592 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXWLG010000178 DAXWLG010000000
VERSION     DAXWLG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.182"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            33..1094
                     /locus_tag="ACGE4T_002434"
     CDS             33..1094
                     /locus_tag="ACGE4T_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFF8387646.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="ACGE4T_002435"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="ACGE4T_002435"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFF8387647.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="ACGE4T_002436"
     CDS             2007..3989
                     /locus_tag="ACGE4T_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFF8387648.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACGE4T_002437"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACGE4T_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFF8387649.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1484 a          818 c         1041 g         1249 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg ta
//
LOCUS       DAXWLG010000179         4537 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXWLG010000179 DAXWLG010000000
VERSION     DAXWLG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.184"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(112..342)
                     /locus_tag="ACGE4T_002438"
     CDS             complement(112..342)
                     /locus_tag="ACGE4T_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFF8387650.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(572..1390)
                     /locus_tag="ACGE4T_002439"
     CDS             complement(572..1390)
                     /locus_tag="ACGE4T_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8387651.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1551..2240
                     /locus_tag="ACGE4T_002440"
     CDS             1551..2240
                     /locus_tag="ACGE4T_002440"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8387652.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2254..3756
                     /locus_tag="ACGE4T_002441"
     CDS             2254..3756
                     /locus_tag="ACGE4T_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8387653.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3769..4245
                     /locus_tag="ACGE4T_002442"
     CDS             3769..4245
                     /locus_tag="ACGE4T_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8387654.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4404..>4537)
                     /locus_tag="ACGE4T_002443"
     CDS             complement(4404..>4537)
                     /locus_tag="ACGE4T_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8387655.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         1493 a          655 c          806 g         1583 t
ORIGIN      
        1 aaaaggatca taaactttta acaagtgatg aaatgggtgg aaattgattc ttatagacag
       61 tttgtctcat aacattgaag tattttgcaa ttttagcatt gaaacacttt tttaatcttc
      121 aacgttgttc ttataaacaa gatgattggg aatcttttga attaacactg tatagcttag
      181 aacacccttt atagaaaaca ttcgcttagc aatcttaata attccttgtg cccgatgtcg
      241 tttagattca gtaggttcct gctcatcgat catggaaaaa atttgaagga tcagatcctt
      301 aataaattta tttaagagag gatgatccat tgcttcatcc atactcgata aactagtgat
      361 aatgagctac acgtttttct ttcaagttga tcaatagctt ccacgataaa aatatcttta
      421 gataaaatca agaaccgtag gaactacagg gatatttggt caataagagc caccgctatt
      481 agtaaaaaat aagctatcaa gtatgctctc atcccaagaa ttgcctactt caatcgttaa
      541 aaaccgttag gtttagctaa gtgggggtag ttcactcaat acttttatca ataatagagc
      601 gcaaaacttt ttctcttaat tcaactgaat caatattatt gttttgttca taaccatgaa
      661 ctaatagatc acaaatataa agttgtgata tttttccagc taaagatcca ccattaagaa
      721 attcttcaat ggctgtttgt aaaactaagt ctgcagactt tcctataggt gaatgaatat
      781 agttagttat agcaataatt tttgcgccat tttttttagc tattttcaat gaatcataag
      841 tatctttcgt tttcccagat aaagaaaaaa tgattactaa atcattaact gttaataaag
      901 aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt tgcttgaact cctacgcgaa
      961 gaaacatatt ttctaaatca agactagtat ttccactaga tcccactcca aatatatata
     1021 tgttttttga ttgagtgatc attttaatgg cttcattcaa tttttcttcg tctaaaagcc
     1081 taatagtgga atagagagct tctgttaaac tattagcaat ttcatcatag tactttacac
     1141 tagtcggatg ttcttttttt tgactaaagt cttcctttgc aatttcaatt tttaaatcag
     1201 aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat agtagcatcg ccaacttttg
     1261 tgtttttctt aatatcactc atcgtactat aaataatagt tttcccagaa tttaaaataa
     1321 aatcagcgac ttttttttca gacttcgtta aaaatggata attttcttta attaaataga
     1381 tataactcat acaaactcct ctacaatttc aacttttctc cattatactc caaaataata
     1441 aaaaaaactc ctaaatactc catttttatc tttactttta tgaaagcgga tgctatattg
     1501 tttttggagt tgttatccaa aaataataaa aaattggagt gtttaggata atggataaaa
     1561 agaggttttt agatcttatt tcaaaaggtt tgattgtttc ctgtcaagca ttacctggtg
     1621 aaccatttta caaagaggaa gaaagtttaa tgccttattt tgctttggct gctcaaaggg
     1681 ctggagcagt tggtatacgt gcaaattctg ttcgagatat tattgaaatt agaaagtgtg
     1741 tatcattacc aatgattgga attattaaaa gagattatcc accagaatct ccattcatta
     1801 ctgccactat gagagaagta gatgaattag tagaaaccgg tgttgaggta atcgctcttg
     1861 attgcactct tagaaatcga catgatggac tatctataaa ttcttttatt actaaaataa
     1921 aagaaaaata tcctcatcag ttatttatgg cagatatttc tacatttgaa gaaggcaaaa
     1981 atgcatataa agcaggaata gattttgtag gaactacttt aagtggatat actgaggaaa
     2041 gtaaaaaaca agatgggcca gatatagatt tgattaaaaa attggtagaa gaagggattt
     2101 gtgtaattgc tgagggaagg attcatactc cagaacaagc aaaagagata aaaaaaatag
     2161 gagttgcagg tattgttgtg ggtggtgcta ttactcgacc acaggaaatt gcatcaagat
     2221 ttgttaaagc attaagttag ggggagaaat aaaatgttta aaaaattttc acaattagga
     2281 cgcgctttca tgttaccaat tgctatatta ccagtagccg gattactatt gggactggga
     2341 ggagcgttaa caaatgagag tgctataaat gcttatccat ttttagatca accgtggttg
     2401 catacaattt taagtattat gagttatgct ggaaatgcag tttttgcaaa cctagcacta
     2461 atttttgcaa ttggtattgc tgtaggactt gcaaatggag ataaaggaac agctggttta
     2521 gctggtggag tagcttattt agtatatact gcaactatta gtgggtttct tgaacttttt
     2581 tcagcaaaag atgcaacact agatacaggt gtagtgggat caattgttgt tggtggtact
     2641 gttgcatttt tacacaatcg ttatagaaaa attgaattac cacaattctt aggttttttt
     2701 ggaggatcaa gatttattcc gattgtttct agtttagctg caatcattat tggttctttc
     2761 ttttatttaa tttggccacc aattcaaaat ggtttagtag ttgctggtga acaaattgcc
     2821 caaatgggta gtttaggaac ttttttatat ggttttttat tgcgtttaac aggtgctgtt
     2881 ggactacacc acaccattta ccctttgttc tggtatacat ctttaggtgg taccgaaact
     2941 gtagctggat caacaattgc tggagcccaa aatattttct ttgcccaact agctgatcca
     3001 aaccacactg gtctgtttac ttatggtact agattttttg caggaagatt tgcaacaatg
     3061 atgtttgggt taccagcagc atgttatgca atgtatcgtg caattcctaa gaaaaatcgt
     3121 aaaaagaatg gtgggcttta ttttagcggg gcccttacat catttttaac tggaattact
     3181 gaaccagttg aatatatgtt tttatttgtt gctccttggc tttatgtgat tcatgcattc
     3241 ttagatggat tatcttttta ttttgctgat atcttaaata ttcgtgtagg gaattccttt
     3301 tcaggtggtt tgatcgactt tttattattc ggtgtacttc aagggaatga caaaactaat
     3361 tggttaaaat taattccttt tggtattgca tgggcattta tatatttctt tgtttttaca
     3421 ttctttatca aaaaatttaa ggtagctatt cctggaatgg aagaagaatc agaagttcat
     3481 tctgaaaaag taataaacaa aaatacatca ttacatgaag agtctcttat cattattgaa
     3541 gctcttggtg gaaaagaaaa tattgagtct gttgaagctt gtgcaactcg tttgcgtgtt
     3601 gctgttacga atggggagat tattcaaaaa caaacaataa aagacactgg tgcaactgct
     3661 gtcttcgaag tgaagggtgg catccaagct gtttttggag ggaaagctga cttattaagt
     3721 caagaaatca atcaaatatt aggaaaagat aattaggagg tttttaatat gtttgaaatt
     3781 ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt ccggaaaaat catttccatc
     3841 acagaagtaa atgatcctgt cttttcacaa aaattactag gtgatggttt tgccgtcata
     3901 cctgatggtt caaaaattta tgctccattg gctggtcgag taacaaatat atttcctact
     3961 aaacatgcta ttaatataaa aagcaactca ggcattgatt atttgattca tattggaatt
     4021 gatacagttg aattacaagg agagcctttt tcgatttgtg ttcaagaaaa tgatgaggtt
     4081 actcctgaaa cctcgttggt tgatgtggat tttgacaaaa taataaaatc tggtaaagat
     4141 acttctgtta tagttgtttt tccggagaaa aatcagttca atgatttaat aatagataat
     4201 ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt tttaaaaaag tgtatataaa
     4261 aagctataaa ataagctaaa taggatattt gtcaaatgaa gttggtgaac agaaagaact
     4321 gttttctttc tgttcatttt tttattttat tagtaatgat tccccatgcc aaatcatgat
     4381 ccgtaattta aagttatgat ttgtcaaatt aagctagaat aaaatgcttc ttgtacatag
     4441 ctcaaaaata tttcgattgg tgttctgtaa ttcaatgatt ttcttggaat atgattacgt
     4501 tgattgctga cactggaaat aaatgtctga ttcactt
//
LOCUS       DAXWLG010000180         4507 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXWLG010000180 DAXWLG010000000
VERSION     DAXWLG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.185"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(197..700)
                     /locus_tag="ACGE4T_002444"
     CDS             complement(197..700)
                     /locus_tag="ACGE4T_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8387656.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1059..2084
                     /locus_tag="ACGE4T_002445"
     CDS             1059..2084
                     /locus_tag="ACGE4T_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8387657.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2407..3286
                     /locus_tag="ACGE4T_002446"
                     /pseudo
     CDS             2407..3286
                     /locus_tag="ACGE4T_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            3762..3878
                     /locus_tag="ACGE4T_002447"
     CDS             3762..3878
                     /locus_tag="ACGE4T_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8387658.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1482 a          729 c          817 g         1479 t
ORIGIN      
        1 ctttaagcta aaaaaaaacc ctttttgtta aataagggga atatagcctg tcagtttcaa
       61 actgcaagtc gtcctgctgc aataatatag tagattagag atgaaaatag atttttttaa
      121 accaatgact gaataaaaag accgtcatag atacacattg gaatgtgtct attacggtct
      181 ttttttacga acgattttat tgaagacgga cagttcgggt caaaaataaa aaagctacga
      241 tcagaaataa gaataaaatg tatcccaaaa taatggctag acggtctaac ttatttgtcg
      301 atagatgttt gtttagaagt tgataggccc ataaacctat cagtcctcct gttgtattag
      361 ttatcaaatc agtgatgtcc gctacgccaa taccgaatag gaattgaatc aattctgcca
      421 agaagcttac aaggaaaata atccctaatt ttttagaaaa agaaagtctc ttgaaattga
      481 cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata
      541 ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag attgacattg ctgtaagaat
      601 aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa aatcaaccat aatagaaaga
      661 ataaataaat ccctaataaa aattttgata cataccgcat agacatctcc tccatttaaa
      721 aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg
      781 tagttatcgt acgaaaatta gaagcttttt tttgttggga atccggtttc tttaacaaag
      841 ctaagatagc gagttgcttg gaaaagttgc tatctttttt ctgtcaaaac gactcggaat
      901 gttcgttttt tgttctaatt tctccaagtt gtttactttt tgatactaag tttatgaaaa
      961 atcaagaaat agttgttata ctaggtaatg ctgacaaacg ggattggcat ttcatcttgg
     1021 ttcagctttt tttccaatta ctaggaagga gggatagatt gttcaagact tattttcgtt
     1081 tcaataaaat acaagataat ccttttcgta tcatgaatct gatgctactt atctttattg
     1141 tattatggtt aggttacata aatcatgatt ttttgatttc aagtttttca tcattaggga
     1201 tctttacttt tttctattat caaaacatac cgatgaaata tttgctgaaa cgtatgtttc
     1261 tgatcggttg cggactgttg gttgctttcg ttttggggat cttatcgact tatgttttat
     1321 ggcttgaacc atttgccgta gctttggttg ctttttccag cagattcgtg ttgcgattat
     1381 tccatatttc taaaccaggt ggcttgtttt ttgcgatgct ttctgccatg ggaacgagca
     1441 tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta tttttttatg ggtgtcgttt
     1501 ttgccctgat tgcggcagtg attacaaaat tactggattc acggcctgag cagacaattg
     1561 aaaaagcaac tttaaaagaa agatttcacg aagagccttt agtgatcatt gattctgttt
     1621 tctacagcgc tgccttattt ctttccgttt atgtcagtca cggcttgaat ctgcataatc
     1681 cttattggtt gaccttgtca tgtgcatcga ttttgctagc agaaaacttg gatgcgatga
     1741 agcatcgaca agtccaatat ctgattggat cgatgggtgg gttatgtgtg tcggcattct
     1801 tgtcatttgt gccgtttacg cagttgcaga ccatttttct gatcactttt ctatatggaa
     1861 tcgcacaatt tctagttgct agaaattatg cagttgctaa tatttttctt aatccgatgg
     1921 cgttgatgct atctacactt attcgtggag catacttgat ttctttgatc gaatatcgtt
     1981 ttttagggat cgtcatagga agtttcatcg gtttaggtgt tgcgtgggta atgacggttg
     2041 gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga atagaaggac aaatatatgt
     2101 gccaaaagag gttatgacaa gatttttgtc acaacctcta attcaaggtg ttcaaaagcc
     2161 agattctcag cattaagtca aattcacaca aaaatgtgga aaggaacttt caaaaaaatt
     2221 ttatatttat tctatcaaaa aattttgttt atgatggtaa ctgaataaac tttcctgaga
     2281 tggtcaatgc tttccttgaa agtcttacag atattctttc ttcatagaaa gtttaagttt
     2341 tatagttagt gtacaataaa agtatgttta taccatatgg tgtccggata atcagtcatt
     2401 taaacaatga ggttcaaaga tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa
     2461 atccaaattc tggcagaaac tttaactctt cccaaagtcg ttatagagca aatgattgta
     2521 caagcaaaaa acatccttga tgaggtgttt gagcaatata gtcatgaact aatggatcct
     2581 aataaagcca aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc
     2641 agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt
     2701 gaagattcca agaactatct atttggcaac aatgaatgct tttactagat ttattcatga
     2761 acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac
     2821 tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg
     2881 gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc
     2941 agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact
     3001 ttattttcct gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa
     3061 tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt
     3121 attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc
     3181 ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct
     3241 tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt agtacttcga
     3301 aatagtttaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     3361 gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
     3421 gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc
     3481 tcttttttag tgttctatcg aacacacagt acgttcaata ggtaaactag ctataacaat
     3541 agatattaaa gttaacggac aagagggcct tttttaggta gctaaaacgt aataatgaaa
     3601 atttatttcc agagatagga gtcaatcagc tagtctgatc gactgtttta acaagttgac
     3661 tttcatttta acttttgata ccatttaaaa cacaaatcgt aattgtttcg atttgtgttt
     3721 tttagcatga ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa acagatatct
     3781 caagtgcata ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt gcgctgaaga
     3841 tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac ttaattaaaa
     3901 aaatagccga aaaacgtttt tcggctattt tcaataatcg gtttttgtgt aaggtttccg
     3961 aaagaactgc tgttgatttt ccatttttaa catatgataa attggtagaa tctttctttt
     4021 caaattgata ccattcccta attccagtta tatagttaac tacagaacca caaagcacgt
     4081 acgtttataa aagtattatc tgtagttatt tttcaattcc ctttcaacct cttattttga
     4141 ccagtagtat tgatcacttt accttttaac acctttgttc gtttttgaaa catttttagt
     4201 caagaatgga aacgtaacta cataatcaaa ggttttgcga aatatttcat gaaatatttc
     4261 atgtagaagc gagcaatacg gttgcaaata accattatcg tttaataagc agttacgaaa
     4321 gctcccaata tctctaacgt tcagctcact ccatttcttt tcttcaaaaa aatcagtgat
     4381 tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg aaaaaatatg
     4441 caaaaaaata aaaaagcagt tactattgct agaataacat caaggaataa taagttttgg
     4501 ttttcag
//
LOCUS       DAXWLG010000181         4461 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXWLG010000181 DAXWLG010000000
VERSION     DAXWLG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.186"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACGE4T_002448"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACGE4T_002448"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFF8387659.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACGE4T_002449"
     CDS             2298..3221
                     /locus_tag="ACGE4T_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8387660.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACGE4T_002450"
     CDS             3237..3776
                     /locus_tag="ACGE4T_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFF8387661.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1663 a          668 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg attgctacag
      421 gcgctacttg gcatagtgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAXWLG010000182         4427 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXWLG010000182 DAXWLG010000000
VERSION     DAXWLG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.187"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..1120
                     /locus_tag="ACGE4T_002451"
     CDS             <1..1120
                     /locus_tag="ACGE4T_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFF8387662.1"
                     /translation="DINNRLARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVD
                     RNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIY
                     PSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDY
                     NDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYD
                     IDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNED
                     IFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYE
                     GYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(3055..3720)
                     /locus_tag="ACGE4T_002452"
     CDS             complement(3055..3720)
                     /locus_tag="ACGE4T_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387663.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            3900..>4427
                     /locus_tag="ACGE4T_002453"
     CDS             3900..>4427
                     /locus_tag="ACGE4T_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFF8387664.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDV"
BASE COUNT         1490 a          682 c          825 g         1430 t
ORIGIN      
        1 agacatcaat aatcgtttgg cacgaaaaag agcggaattc aaacaatttg ggaaacaaat
       61 cgatttagcg attgtagtgg atcgtctatt aacaggtttt gacgccccta cgattcaaac
      121 cctatttgtg gatcgtaatc tagagtatgc aggattgatc caagcttttt cacgtacgaa
      181 tcgtacgtat cctgataaga caaaaggatt gattgtgacg tttcgtaagc cagcgacaat
      241 ggagaagaat gtcgaggatg cgacaaaact gtattcaaaa gcaaaagaag aatcaggttt
      301 gatttatcca agttaccaag aatccaaaaa aagattcaaa caagcatatg gtaagttaaa
      361 agaggtttca tccatggaat ccattgatga acatacgcca cttgaaatcc gtatcgagta
      421 cgtcaaagca tttcaagaat tgaatcgtgc ttatgaatcg ttagtgactt atgacgatta
      481 caatgatgac atggtgaaat ctccaacttt gaacaatcaa attcaacttt tggaagaaca
      541 aattggcgtg tacgagaccg tgaagggttc attaatagag gaagaaccag aaaaagaagg
      601 agaagatttt tcaacgatcg aattttatag tgaaaatacc actaagttgt atgatattga
      661 ttcaacttat attgatcaat tactaggaac ttatgctgcg aatagttcag atgcacgaga
      721 agaaattgaa aaagctttgg ctaaattgaa taaaacagaa ggagtcaaag aagtttaccg
      781 acaaatttta aatgcatttg atgtggggac tttagatagt aatgaagata tctttgtcat
      841 taaacgtcgc tattttacac aagcgagtga tgatttgatt catgtctttt ccaatgagtg
      901 gtttgtttct gagaaagaat tacatgcttc taatctgcaa tatctaccag gtgaagatcc
      961 aattcctaat atgaaagcaa ttatcaacag taaggattac gaaggatata aagccaaaca
     1021 tccagaagcg aagccattta aatacccaca agaaatgaag cgagcatggc gtaaaatgtt
     1081 agatgatgag ttgattccac ttgagaatga attgaggtaa acgagaagag ttaagaaaat
     1141 tgtacgattc aaagatggta gtcaagtgga agtgtagaaa atagggatag atgtctttta
     1201 tttgttcaca ttttataacg aagtatttaa gacagctatt gaataatgtt tcctccacct
     1261 ttttcattca tttaccccac cccgttgtta tatgaccgtg ttttgagagg aaccaataaa
     1321 aagcataggc taactagggg ttcctttttc ttttgttttt ttggaatccg cttaagtgaa
     1381 atgtatctga aatgaaacgg agataaagtg attttttgct ataatgacaa ggttttagct
     1441 gtttcagata ccatgtaacc caaaatagtt tattgagaaa ataggagagt atttgatact
     1501 aactagtagg aaaaaatgag ataaaaatta aatggaatca tttcaggtaa tctcaaataa
     1561 aaaaatatac agtcctgtgg cactttacct atgggactgt atatttttta ctctcagcgg
     1621 gcttgttttg actccgtgtc tgactccgtc gtattgattt taaatgatat tcgatgacca
     1681 tttaacaggt catttttgaa atttttgtct ctatatgact cagtttgata attattgata
     1741 gatcaatttg ggtctatatc gaacaaggga ataatccaat aaaaaatgat ttagaatatc
     1801 aaaataacaa aaaagaggac tacctgtatt cgtcctcttt tttgaccaga ttttgaccag
     1861 tctaatttac attctaatat attcatatga gctaattttt tgattaaatg gtaagttttt
     1921 ccttgtttgt tttagtttaa actagtgtgt aatttaaaaa tctatatgga agggtctgtg
     1981 gggtaatact taaataaagg ctaggaacct gataacatca aggttcctag cctttttata
     2041 ataaaatgaa gcgaaaataa tttcttttaa agagccgaga ttatcagcta tataagagtg
     2101 aatttgatgc aaaaaacaga gccgaaacag tcttggtgtt cgtagctctg cttatatgtt
     2161 tcgaatttaa gacaaagagt tggcaaaaga tgttgataag gaagataaga taactgtgtt
     2221 atataaacta ggtaactaac tgtaacaagc taattttaag agattcttat tagaagacgt
     2281 ttaggaatat aactaagaaa atgattatac aggtaattgt catttcaata atgttttcta
     2341 attttcgcgt ctttgtttta agaaaaaggc cagaatcagt tggaaaaaag ttcgtacgat
     2401 agtgaagagg ggcaaaattt cctactaata ttcccactaa caagagataa aacggccagg
     2461 ttggtgtgat tcctctataa aaaaatgccc atgctaccga aatcccatag attaacaact
     2521 gaacaatttt tacaatgaaa aaattgttct gccattactc aaagtctctt cctccttgta
     2581 tcgtttattt gtttcatttt atcattatga taggcgcttg aacaatccat tttgtatttt
     2641 attgagtact cttggataaa ctaccagcga ttatcatatt tatttatgtt actgtaagcc
     2701 atcatataaa agtaaatgag cagatcatag aaatataaaa aagaactaaa attgatctaa
     2761 attatgtgca gcttgactac gtaccaatct atattactta ctagattggt cgttaatcta
     2821 ttcggatcga atgagcaaaa tatttttgtg aatgtccatc cttgattatg attccaaaaa
     2881 gaatcattgg gaggctgtgg cgtaaatatt atactataag ccagaaatac ctatcattct
     2941 gaactttttt atttaggaac tttcatctca aggctttttc aattaatttg tacccctgtt
     3001 ttttggataa gatcatgtgc attgattttt tctatctcta cgttcatctc ctacttaatg
     3061 cctatctggc tcctgagggc ttctttttaa ggcgtcgtca tagtcttggc tgaggatttc
     3121 ttggtaatct gagggaatgg cttcccaagt gagcaggcga acgttcatga cgtgttttcc
     3181 tttgtgtttg attacagcat ggtttttctc tttttctacc tctccagcgt gaaaagacag
     3241 ccatgtacca tggccaaaga tatcattaca tacccaacgt tcgatggaat tgtccacgta
     3301 tcctacaggg agtgtggtat agccgatttg tggtttcaaa aaaggattga atcgatcgat
     3361 cgtttcattt ttgtattgat aattgataag aaaaagatag accaaacaaa agacgagcag
     3421 aaagaaagat agtgtctttt tgagtgagaa gggttgtttt gatgaataag atttcttgtt
     3481 aacaagaggt tcatctggca atgttggttc tattgattcg gtagctgatt caatagaagg
     3541 aaaatcaatc ggcagaccta aagtatgatg taaaagtgac agcatttcaa agtctggcat
     3601 tgtacgagaa ttttcccaac tagaaatcgt tttatcggaa acaccaattt tttcgcctaa
     3661 ttctttttgc gtaagcttac aatttctgcg gtaagttcgt attttttctc cggtgttcat
     3721 cttcttctcc tttcgattct acagtatata gatttattct acatgaccta tacacttgat
     3781 agatacgttt ttttgatgaa aaacggtatg attttatcaa agaatcattt aagtaaggga
     3841 gagtggtgat aatcaaaaaa aagttcacag ttttactcac aatcatagtg accggtatca
     3901 tgagtggttt tgccttggtt agtataaata aacaggtact ctatcggaca aatgaatttt
     3961 tccatcaaac agatacttat actccgattc gattgccaca acattttatt gggcaagaag
     4021 aatatgagca gctaatcatg gtatttgaaa atgtctcatc agaaactgag ctttcttata
     4081 tgaaaaaaac agtaaatgaa tccaatgaag tagatacatc ttggaacttc aagatacagc
     4141 caaagcttga aatgacattg tattatcaag gggatgtcaa atcttctcac gagaaggtac
     4201 cctattctaa tacctccttt cgcgtaacgt ccattgaaga atcgatcaaa ataaaagatt
     4261 ttgaaggtga atttttcttg atgacgagag aaaaagaagc ctatgaacgg gtcatttcac
     4321 tattcactga tcgattcaat caagcatttc acactgatta tgccactaat atttttgaag
     4381 actttgaaaa tagctattcg tattcggtta tttcaaatac agacgta
//
LOCUS       DAXWLG010000183         4418 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXWLG010000183 DAXWLG010000000
VERSION     DAXWLG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.188"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            59..913
                     /locus_tag="ACGE4T_002454"
     CDS             59..913
                     /locus_tag="ACGE4T_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8387665.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACGE4T_002455"
     CDS             1010..3259
                     /locus_tag="ACGE4T_002455"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8387666.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4418
                     /locus_tag="ACGE4T_002456"
     CDS             3262..>4418
                     /locus_tag="ACGE4T_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8387667.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          625 c          807 g         1430 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagt
//
LOCUS       DAXWLG010000184         4398 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXWLG010000184 DAXWLG010000000
VERSION     DAXWLG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.189"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            276..545
                     /gene="rpsN"
                     /locus_tag="ACGE4T_002457"
     CDS             276..545
                     /gene="rpsN"
                     /locus_tag="ACGE4T_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8387668.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            560..739
                     /gene="rpmF"
                     /locus_tag="ACGE4T_002458"
     CDS             560..739
                     /gene="rpmF"
                     /locus_tag="ACGE4T_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8387669.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            771..1148
                     /locus_tag="ACGE4T_002459"
     CDS             771..1148
                     /locus_tag="ACGE4T_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387670.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1169..1318
                     /gene="rpmG"
                     /locus_tag="ACGE4T_002460"
     CDS             1169..1318
                     /gene="rpmG"
                     /locus_tag="ACGE4T_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8387671.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1342..2325
                     /locus_tag="ACGE4T_002461"
     CDS             1342..2325
                     /locus_tag="ACGE4T_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFF8387672.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHREERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKHYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2355..2477
                     /locus_tag="ACGE4T_002462"
     CDS             2355..2477
                     /locus_tag="ACGE4T_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8387673.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2495..4018
                     /locus_tag="ACGE4T_002463"
     CDS             2495..4018
                     /locus_tag="ACGE4T_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8387674.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4042..4281
                     /locus_tag="ACGE4T_002464"
     CDS             4042..4281
                     /locus_tag="ACGE4T_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387675.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSV
                     DTVHRALKKDPDCCFIKVNTGRSRRLAKRYNILIN"
BASE COUNT         1638 a          631 c          876 g         1253 t
ORIGIN      
        1 tagaggaaaa gctacttaaa acggtatttg tgtttaattg gactaataaa agtgaattct
       61 tatttttcag agcttaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga
      121 taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt
      181 gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag
      241 atgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta
      301 aagcaaagaa acaaatggcg atgattgaaa agtacgcaga taaacgtcag gaattaaaag
      361 ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga
      421 ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc
      481 gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt
      541 ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg
      601 gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg
      661 tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat
      721 taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta
      781 tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc
      841 tagataaatg cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt
      901 taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct
      961 tatcatttga tgatttttta agaatctatc caatttttga agagtatgac gaggagttat
     1021 ttcaatgtat tgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac
     1081 aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag
     1141 atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga
     1201 aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga
     1261 attaaaaaag tattcgccaa aattgcgcag acgggcaatt ttcaaagaag tgaaatagct
     1321 aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg
     1381 gccggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa
     1441 attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat
     1501 agagaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat
     1561 ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca
     1621 caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca aacactttta
     1681 acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt
     1741 gacagttttg aacttattga aagagaacca caagccctta agcaacttgt catggcagat
     1801 cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga
     1861 gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacgcatag tgaaacgatt
     1921 caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat
     1981 tcatcagaaa atcactataa tggcaaacac tatcatcaac accatcaagt acatgagttt
     2041 aaatcaattc tcttaacttc agatcaagat ataaaaaaag attttttact ccaatggatt
     2101 gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta
     2161 caagagcagg attttatagt tgccatgcaa ggggtaaacg aacaggttaa ttttcaatac
     2221 accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa
     2281 aggatcaaac agagttttga taaattactg gcggaaagca aataatgata agtattgatt
     2341 gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc
     2401 cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa
     2461 tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat
     2521 tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc
     2581 gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac
     2641 caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc
     2701 tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt
     2761 atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc
     2821 tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga
     2881 agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc
     2941 acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc
     3001 gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt
     3061 ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacacaaca
     3121 tgcagccttt ggatatttag ctttggatta tggattgata caagttccta ttgctggact
     3181 aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa
     3241 aaataagatc aattatattt actttgaaaa gaatgctaat gataaaatcg caaaaacttt
     3301 ggccaatgaa gctgggataa aattggaagt attgaatcct cttgaaagtc ttacaaaaga
     3361 acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga
     3421 gaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa
     3481 gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta
     3541 tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt
     3601 cgattacaaa gcaaaactca ataaagacat gacagctgcc gagtacaaag attattatac
     3661 tactggctat aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt
     3721 agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta
     3781 tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg
     3841 ttttaaatat attcaattta gcgatcatgg aattgcacca agtaaggcag agcatttcca
     3901 tattttcttt ggtggagaaa gtcaagaaaa actttataat gaaatgcata attggcctac
     3961 tttctatcca gcttcattat ctgagcatga gattgctcaa gaaatgatgg cccattaaaa
     4021 tccaatcttt ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat
     4081 attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta
     4141 gctgatattg ctgcaacttt acatgttagt gttgacacag ttcatagagc attaaaaaaa
     4201 gatccagatt gttgttttat caaggttaat actggaagaa gtagacgttt agcaaaacga
     4261 tataatatct tgataaacta attgtaacac taaagcagtt caattcttcc ttaatggaat
     4321 ttgatctgct ttttattatg catagaaaga ggtttcctat gaaaaatatg tcacaattct
     4381 actagttatt ttgattta
//
LOCUS       DAXWLG010000185         4279 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXWLG010000185 DAXWLG010000000
VERSION     DAXWLG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.190"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..695
                     /locus_tag="ACGE4T_002465"
     CDS             <1..695
                     /locus_tag="ACGE4T_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFF8387676.1"
                     /translation="ELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNFGQ
                     MFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKN
                     TVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKES
                     RAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQL
                     NRQQRLFGDDNE"
     gene            873..1214
                     /locus_tag="ACGE4T_002466"
     CDS             873..1214
                     /locus_tag="ACGE4T_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387677.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1207..1878
                     /locus_tag="ACGE4T_002467"
     CDS             1207..1878
                     /locus_tag="ACGE4T_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFF8387678.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1884..2570
                     /locus_tag="ACGE4T_002468"
     CDS             1884..2570
                     /locus_tag="ACGE4T_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFF8387679.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            2573..3322
                     /locus_tag="ACGE4T_002469"
     CDS             2573..3322
                     /locus_tag="ACGE4T_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387680.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            3334..3603
                     /locus_tag="ACGE4T_002470"
     CDS             3334..3603
                     /locus_tag="ACGE4T_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387681.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            3608..3772
                     /locus_tag="ACGE4T_002471"
     CDS             3608..3772
                     /locus_tag="ACGE4T_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387682.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            3765..4067
                     /locus_tag="ACGE4T_002472"
     CDS             3765..4067
                     /locus_tag="ACGE4T_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387683.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            4064..4225
                     /locus_tag="ACGE4T_002473"
     CDS             4064..4225
                     /locus_tag="ACGE4T_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8387684.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
BASE COUNT         1617 a          679 c          925 g         1058 t
ORIGIN      
        1 aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa gtggcagaaa
       61 gcttcgagaa aaaacatcaa catgttttaa gagatattga tgcactaaaa aaagatgtgt
      121 ccaattttgg acagatgttt gtagaaggta atgaaccaga ttcatatggc agaaatcgac
      181 gagttttctt cattagtaga gatggatttt tcttgctggc tatggggttt acaggaaaga
      241 aagctatcta cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttattc
      301 gcaagaatac tgttcctcaa acaattgaag atatgatgat ctatcaatta gaagaaatga
      361 aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag
      421 agtttgaaat taagcaaaaa ggaaatatga aagttatggg agttctaggg ggaaaagaaa
      481 gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac
      541 gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt
      601 catttattga aatgtggttg ccagaaactg cgatccgtat ggaaattgat caactgaaca
      661 gacaacagag actttttggt gatgacaatg aatagagctg aagcgctaag aatagggacg
      721 gcaattgcta attgctggtg gaaatactat aaaccaatca tcctaagcca acaacatatt
      781 gacaagcaaa aagcatggca acaaataaaa aagtgactcc gccggcaagc aaagagtcac
      841 aaagaaaaca catcataagg agattttagc atatggaaaa agaactttcc actctagatc
      901 aatatttgat tgatcctgat tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa
      961 tcagacgaaa tcttttgacg aatgaagaac tagcttggga tcaagatgat ttaggcaacc
     1021 atgtaactat ttgggatcat gtttatctta tccatctatc gaagcattcg aataaacctg
     1081 aatatattta cgtcatcgaa gatggcttga ttgatgcgct agaagagtac gacagagata
     1141 acttgattga tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag
     1201 aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag tcgtaagtta
     1261 aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca cgcgtgggag
     1321 attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtacaa acactacagg
     1381 gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt tcttatggat
     1441 gaaactggga cttatgtatc tgtctcagta acggttaaag gacacacgga aaccgaatta
     1501 tttcctgttt tagattatcg aaaccaacca gttgttaaac caaatgcaat gcaaatcaat
     1561 aactcattga agcgatgctt tgtgaaagca ttggctctac acggactggg attatatgta
     1621 tttcaagggg aagatattcc aacaccacct agaatcgata caaagaaatt aaacatgcta
     1681 gagacgattc tagaagcttt caatgagcag atgggtaaag atatgaccaa aaccttaatc
     1741 gaatatgtta atgagcagac agataaatta gggctcttag ctgataacgt tgaaactatt
     1801 gaacagttaa gctatgagca atgtgccttg atggagcgag caatagcagc taagagaaaa
     1861 gaattagata agaagtgata tgagtgttta aaccattaat cgattcatat tcagcggttc
     1921 tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa tgaggaagtg aacattgatc
     1981 gactaaagac gatgtatgac ggctacgatg gtgatcgagt cattgaaatt cgttttattg
     2041 atcctagacg tttcaccgta cagcaacgaa acttcatcta tgcgctgata ggcgatattt
     2101 ttatcgatac aggcatgcca acggacttct ggaaggaatt cttctacttt cgttttgaag
     2161 gtgtcacagg gcgcaaaata agcctgaaag acgaatcgaa tacaactgtg agtgatgcca
     2221 atgtcttagc aaatatcatc ttagatttca tctttgaaca tcatattcct ttcaaagaag
     2281 gctatgagat tttacctgcg aatcaagaat attacttcta caaatgcatc acaaaaagag
     2341 tctgctgcat ctgtggcaaa acaggagctg acatcgatca ctttgacaaa gcgctaggaa
     2401 gacgaaagcg caaagaagtt gatcatgcag agtacacatt tgcagcactc tgcagaatcc
     2461 atcacacaga gaagcacaaa ataggtgtga ttaatttcaa aaataaatat caaatcaaag
     2521 ggatcaaatt aaaccagaaa acaatcaaaa agttaaatat tggagggtaa aaatgacaga
     2581 acatcgaagt tattatgcga ttataccagc caacgtaagg tacgacaaaa gacttaaacc
     2641 aaatactaag ttgttatacg gagagataac ggccttgtgt aatgaaagag gcttttgttg
     2701 ggcaggcaat gagtactttg cagatttata tggtgtgaat aaagagacca tatcgcgatg
     2761 ggtaagtgat ttgattaagt ttggatactt gaatcgggaa atcatttaca aagagggtac
     2821 caatcaaata atcaataggt acctacgaat taatcaatac cctattgacg aaaaacgcaa
     2881 tacccctatt gacgaaaaag tcaaagataa taatacatct attaataata catttaataa
     2941 tacaaaagaa tatataagag agttaccgcc ttcgaaaaaa tcgaaggcta agcccgtccg
     3001 tcataaatac ggagagtata aaaatgttct tttgtcagat gagcagatgg agaaactcaa
     3061 aacagaattc cctaatgatt accaagagcg aatcgaacga ctgtcagagt attgtgaatc
     3121 atctggtaag acttataaaa actatttggc aactattcga agttgggcaa ggaaagaaaa
     3181 aagtgaacct aagaacgcaa gcagtggata caagcgcaca ggaagacgag agaagcttcc
     3241 tgaatgggca atcgaccaag aagcctatct caagaaaaaa gcgctagaac gagctaatag
     3301 acaatcaaaa gcaccattct aagaggtgga aaaatgaaga tcgattatct agaactaatt
     3361 aatgaaatag cgaattataa aaagggcgag gaattagacg tcctgagaga cgtatatgat
     3421 caactcgaag aagctggaat tgaaggaatt aagaatgatc gttcgagttg gagtaagctc
     3481 agatactatt tcgcacttta tatcgataca acacaattaa gaaatttagc atataccaaa
     3541 ttactatttg ttgattgtgt taaaggcttg caaaaacatc ttaatgaact tgagcaggtg
     3601 taatcagatg gacctaaaga catttacagc acagattgaa ctaatgcatc aagaagcttt
     3661 aagacaaagt gtatcgtacg aagacaagtg gctcaacacg ttccatggcg gacgtgagag
     3721 cgcacttgat caagtactca aattactgaa aggagaatgt cgggatggat aagaaagcgg
     3781 caatgcagcg aattatcgaa ttgacttatt cagaagattg gcaaaatgac aaagaagctg
     3841 cttcagaagt gatgaggctt ggaagagcga tgtgggcaga caagagcaac aagccaagac
     3901 cacgaaaaat cgcaatttgg cacggtgaca aacttctagt gatagggaca gctgaacagt
     3961 tagcaagtct cacaggcttg cacgagaaaa tcgtgaggaa aagagcaagg tgtggctaca
     4021 cagacgctaa gaagagaacg tttagatacg tggaggaatc atcatgacaa cagaagaagt
     4081 gattcaaatg cgtattcgaa gccttcagcg tgagattgac gatctggaac gaacaaaggc
     4141 agtgatggtc aatgaaacgg cgagaaaggc aatcgatttg cacatagaga atttaagaag
     4201 ggaaatccat cgattggagg aatgagcgtg gataagaaag cagcaatgaa aagaattgct
     4261 gaattaacca agtcagaat
//
LOCUS       DAXWLG010000186         4252 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXWLG010000186 DAXWLG010000000
VERSION     DAXWLG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.191"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            90..3257
                     /locus_tag="ACGE4T_002474"
     CDS             90..3257
                     /locus_tag="ACGE4T_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFF8387685.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            3325..3822
                     /locus_tag="ACGE4T_002475"
     CDS             3325..3822
                     /locus_tag="ACGE4T_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFF8387686.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            3850..>4252
                     /locus_tag="ACGE4T_002476"
     CDS             3850..>4252
                     /locus_tag="ACGE4T_002476"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HFF8387687.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVIL"
BASE COUNT         1448 a          752 c          971 g         1081 t
ORIGIN      
        1 aaatacatat tttaaatatg aaagtacaat tggaaataga ttaaaacgat aaaataaaat
       61 tgtcaaaacg tcagagagga tggtggagaa tgaatcaatg tgtaaaaaag ctgattgcgt
      121 cgattttgtt tgtcttccct ctcttgatgg tgacatccgt ttttgccgat gtagagaaaa
      181 caaacgaatc aacgcaatca gagaatcaaa gttttgacaa gactactttt tcagaagaat
      241 cgattacttc tgaaactaca gaatctcaaa gtgaggcttc tactaataaa aggcaagaag
      301 aaaataagac aagtgagacc tcagaagtag ctgaacaaac gtctgatagc agtgaagcgg
      361 cactggcaag agctgtttca gcggatgggt acagcgaagc tgccacaaca gaagaactgg
      421 cgcagcttct cgcagacgaa tcagtaacta agatccgact gattcagcct ttgactttag
      481 acagggaatt agagatcaaa cgagatatcg ttattgattt tggtggattc gctcacaatt
      541 tcggcacaca ccatatttat atcaatgaga tacctcagtc gatcgagttc cagaatttta
      601 aaggaacagc tgcacatcca ggtggattga caccaaatat cgatggaaat gccattatca
      661 ttgcttatat gaccgatttt tggggaagca gatatcattt tacgggagaa ataaaattta
      721 ccgggacgct tgatttgtat gatgggtcca aattaggact gatttacgct cctcgtgcaa
      781 ctgtcacatt agatggtgtt tccggtgtgt tggatgtcca accagtgaag gaaggactga
      841 atgccgctcc gggtaaagcc tatttttgta gaagctatca attgaatgtg atcaatggta
      901 gtcaacttta tggaccgtat ctagggaaat tctacggttt tctagattca ggagatgaga
      961 ctggtagtgg aaaaacagcg ccaggaatcc atatcttaag cggttcaaag gtctctcttg
     1021 attatgatcg ggcagatggc gttagcgatg aaggagaggc agtcgatacg ctacctggta
     1081 atgtcatgtt tgaagtcagt ggttcgggaa gcgaattttc ggtaaataca aagatcaata
     1141 ttaccaatga caacaatcgc ggaatcatcc aaatgagagg ttcaggatcg aaagtcactg
     1201 tatcgaatgg tgggaaaatc accatcaata cagcaactac gggcggattt cgcttgcaag
     1261 gagattctag tcaaatccat gtatcaagtg gtggacaaat ctcggtcaaa atggctggag
     1321 atgacgatca acccgggaac aatgggatgc gttttgtagg aaaaggcctt tctttgatcg
     1381 tagaaggagc cggatcagtt atcgacatcg ataagcagag tgggcgttcc agcgcagttc
     1441 gttttgagaa tggtacgcaa aagctgacag taaccaatgg aggctcattg agggtaagaa
     1501 atacaggtga tggcaaatct tacgtgaaca gactcaatca aggaaatcaa gcgatccaat
     1561 tttatgacgc ttctggtttt ctagaaagtc caggttcagc tgattttaca gtcactggag
     1621 aaaaatcaaa tatcgatata cgcgcagatt ccggagcaac tgtagataca actggagata
     1681 ttgatttaaa ttttacggca ggtgaaaaaa cttatttagt catggtcggg aaaacagggg
     1741 aagataagac cggcatcatc agtggggatg tgttgaatgt ggacttagaa tctcctacct
     1801 attttgattt ccaaaacaaa cggattggaa gcgagaccaa tggcataggc ggctgggtat
     1861 tccaaggaaa tggagattct tctttaaaga tcgcccattc agaaatcgct ttatggcgaa
     1921 aaacaggcgc aacattcgat aactttgaaa aagacccgga ttatttttct gatatgacag
     1981 acttatcgat caatgggacc gacttaggca atttcgtatc aagccctagt gcaggtttaa
     2041 caaatgaatt tgctcagaca ggtgccggaa tgaaaaatta taatcgaatc agtgccaata
     2101 atcagctccc gatgattgaa tccttaagag tgccaacgaa tgcagataaa aaaatctatg
     2161 ggcatgtcgt tgttcccgaa ggtgtcgagg caaagccaag agatgcttgg acaggcgaag
     2221 tagaagtgac tttacgtatt acttacgcag atacgacgaa atcatctgta gaaattactg
     2281 ctctcacgca aggacaaaca gatgaaaatg aaggggcagg atataaccca tggggagaag
     2341 gcgaacaagg cggcttgttc gaagttcaag tacccaatga tgagagatta aaaacggagg
     2401 atcaagtgca agttgttgct gctaaaaaag taagaggtgg ccaagaagtg acagaactgg
     2461 actctgccaa aacgacagtc gatgtgattc ctcctgaacc tgctaattta agtaccacat
     2521 tgatcaatgc agcttctcgc acgatcacag gtacaggtga agcaggagcc ttggtttcat
     2581 tgaaaaaaaa tcaagctgtt ttgtcagaaa caactattga tgatacaggg gaatttgttc
     2641 tttctatccc agaaaatcaa ctttttgcag gagatgtatt agaaattgta ttaaacgacc
     2701 aagcaggaga agctactgct aagggtgtgc agaacaagcc aagtaccaac gatgaaattg
     2761 ggaatcacaa tccaagtgat acgccagttg tttatcatga tgccccgcct tttgcacctg
     2821 ctacgaagat cgtagtagaa ggtggtctct catttcttgc cccttccaaa ctcgattttg
     2881 gtcagatcaa agcaactggt ttgacacaag aagcatttgg ccaaatggat atacaggaaa
     2941 aattatggat ttatgatcag cgagaaacga aaaatccgtg gatcttaacg ataaaattat
     3001 ctcagccttt cacaaatgat gcaggatttt ctatgcagga tgtcctatat ttcaagaaag
     3061 gagaggagta tcaattagtg aatgaagaag aactggaaat tgtcagagga caaaattcaa
     3121 cgaatgtgca agcagattat tcagaatatt taaataacgg tcgggcgttt aaacttgtct
     3181 taaccaaaga aaatcagata gctggtaatt actcagcaga gataacatgg acattagcag
     3241 atgcgccgac agaataaaga tcaaggagga tgattgatga atcgaatatg gaataaaagt
     3301 catcgaaaca actgctcttt acttatgatc cttctccttg gattcatcct ttggcagaca
     3361 cctgtccatg cctccgaatt caattttgcg gtagattttg aattaccggc taatcagatt
     3421 gataagaaca aaacatatat aaatgtaagg atggagcctg ggaaaacaca ggaaatcccc
     3481 tacacggtct ttaaccgaac agataaatca gtaacagttg agacattgat ccactccgct
     3541 acgacgaatt caaacggtgt gatcgaatat ggagaatcaa aaagtaaatt tgatgaaaca
     3601 ttaagttatg atatgaaaaa cttagcaaaa acagtgggag aaataactgt tcctcctcaa
     3661 agtgcggtca ctgagacact ttcactgaaa gcgccagaaa aagagctggc tggtgtggtc
     3721 gcaggaggga tcacgtttca agaaaaaaaa gagaacgttt ctaacaaaag tacagttggt
     3781 gtcgtcaatc agttttctca agtgaaagca atcttgattt gaaatagcga aaaagagtct
     3841 tccccagatt tgaaattaaa aaaagcttat gcggatcaaa gcgatggaag aaatgtgatt
     3901 aaggcgaatc ttcaaaatcc aaatgcttct tatatgttcg gcttgactac atcggttgaa
     3961 atctacaaaa aagaaacaaa agagccttac ctttcaataa aaaaagaaaa aatagatgta
     4021 gcacccaata caaattttga tttacttgtc ccattagaag gaaaaaaatt agctccggga
     4081 cattatcaag cggttgtgaa gggtgcttgg aaagaaaaac gctggaatct ccataccgat
     4141 tttgaaataa ctgatgatgc agcggaccaa ttgaatgaaa aggatattga tttaaaaaat
     4201 cagccagaaa catttgactg gcgatggctg atacttgttg gagtcattct ta
//
LOCUS       DAXWLG010000187         4249 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXWLG010000187 DAXWLG010000000
VERSION     DAXWLG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.192"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..165)
                     /locus_tag="ACGE4T_002477"
     CDS             complement(<1..165)
                     /locus_tag="ACGE4T_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8387688.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFH"
     gene            complement(162..893)
                     /gene="liaF"
                     /locus_tag="ACGE4T_002478"
     CDS             complement(162..893)
                     /gene="liaF"
                     /locus_tag="ACGE4T_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFF8387689.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(1061..1540)
                     /gene="greA"
                     /locus_tag="ACGE4T_002479"
     CDS             complement(1061..1540)
                     /gene="greA"
                     /locus_tag="ACGE4T_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFF8387690.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(1676..2851)
                     /gene="mltG"
                     /locus_tag="ACGE4T_002480"
     CDS             complement(1676..2851)
                     /gene="mltG"
                     /locus_tag="ACGE4T_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFF8387691.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            3016..4176
                     /locus_tag="ACGE4T_002481"
     CDS             3016..4176
                     /locus_tag="ACGE4T_002481"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8387692.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         1323 a          860 c          668 g         1398 t
ORIGIN      
        1 atggaagata agcggaacgt ataataaacg cgcacgcagc aattctcccc accaatgact
       61 ttggttactg gcataaaaat aagtgaaaag tgaaaataga ataataagga aagccgcaat
      121 ccccgaataa acagctagca ttgctttgga tattcttccc atcatattcg tatcacctcg
      181 acatctccaa ccaaagtatt ggtaatgatc ttcaatctgc gaggattttc atcataatca
      241 ctgctgcaga ttttgatctg ttcgtttttt aaggaaaact gctcttcttc aaacaagaca
      301 tttcctacta atgtcgcatg ctctaaacga atagccactc ccaggggtac taagatccgt
      361 gttctgccta tcccttttct tacaatcaca atattatcat cttttggcaa aagtgtattg
      421 cccaaatcga tgatagtatc accagaaatg atggcaatgt tgatatcatc ccattcatag
      481 acgtcgtttc caatacgttg gttaccaaat aattgctgtc ttttacgctc attattatgt
      541 gtttttaatt gctctgtttg gaccatcatg atctgttttt ttcgccaaaa agcatttttt
      601 gtcaaatcta ttcctgaaat ctctacacct ttcagcccga taaataaaac ggccaataca
      661 accatcatcc ataaagcagg actgttgact aagctaaaaa aaatgaccaa gctccctaag
      721 atcaattgaa aattttgaaa tttagtacga ggatatttgc gcatagctag atatatattg
      781 aaaataccga atattactaa aagcaaaagt ccagtattgt ttacgatttg ccaaacagca
      841 aataacagca gcagtgcttc gactaccaca aaaaaacgcc aagaactatt catcgctttt
      901 tctccctcct gacacattct catttcatca tacctatttt tttgactgtt ggctatagga
      961 ctttagatgt aagatcgttc agtgaaacct gcaatactca aagagaaaag atctagagtg
     1021 cgtcatcagt aagatgaact ctctagatct ttcagtcaga ctacgcttgt tctactttga
     1081 cgattttaac taacatatcg ccacctggtg tagcaatggt tacttcatca tttttacgtt
     1141 tgccaatcaa tgcttgcgcg ataggagaat cattggaaat ctttccagaa aacgggtctg
     1201 cttcagcact tccgacaatc gtgtactctt cttcatctcc atcaggcaac tcgatgaacg
     1261 taactgtttt accgatcgat acctcatctg catctacgcc gtcattatcg atgatttgtg
     1321 caaaacggat catattttca attgttgtga ttcttccttc aacaaatgct tgttcatctt
     1381 tagctgattc gtattcggag ttttcggaaa gatctccgaa accgcgagcg attttgattc
     1441 gttcaatgat ttctccgcgt ttaactgttt ttagttcttc taattctttt tctaattttt
     1501 ctttaccttc tagagtcatt ggataaactt tctctaccat gtgtaacgtc tccttttttc
     1561 gataaaatac cgcaaaaggt aatcttttga ggaatatagt ccaaaaaaga ttaccacgct
     1621 tttcttaaaa tgtaaatgtt tttctaaaaa tgatcaatga ctagcccctg tttttctaag
     1681 aattatttac atatttttgc actagttcca tatgttcttc atacgtttta gcgaaataaa
     1741 catttccagt gctcgtatct gctacgaaat aataatagtc attttctgct ggttctaata
     1801 ctgccttgat tgcctcttcg cttggattgt caaaaggacc aggcccatag ccggtattgg
     1861 tatacaaatt gtatggtgaa tctacagctg tatcttcata cgtcaccaat tctttatgtt
     1921 ctcctaatgc atataaaaca gagatatctg actgaagtgg catatttgcc ttgattcggt
     1981 taaagaagac ttgtgcaata tttttacgat cattttcttt tacgccttct ttttcaacta
     2041 gagaagccaa agttaaaact tcttgtacag tcaaattttt ctgtttgata gctgaataat
     2101 agttttgcat gaccgtattt gttttgttca ccatctcaat cacaagatcc ttaagcgaag
     2161 tactcgtgta gtaatcataa gtagctggga ataagtagcc ttccaaacgg tattttgtgt
     2221 tctcagcttt agaggcactt tccaacaatt ctgggaattt ttgatacaac tcgttaaaga
     2281 aagtctcatc atttactaaa tctaaaaatt ctttcttgtc tttaccagtt accttcgcta
     2341 cccgctcagc aatctgatcg atgtcgtagc cttctggaat ggcaattttc ccatctgcaa
     2401 cttttgttgg ctcagaagtg ccgccttctt gcagctgttt gccaatctcg tcaagagtca
     2461 tattaggcgc taactgataa tagccagctt gaaagccagt taaattttta aattttgtat
     2521 aataattgaa aacaatcccg cttttaataa tattgtcctt ctccaaaatc tcaccaatct
     2581 gcttgttaga agagccactc gggatctcca cttggactaa ttggtcatct gatttatcta
     2641 aaggcttcaa cccgctatct acataacgat acacggtaaa tccaagaaat ccgccaataa
     2701 ttaataatgt aagagcaatc actaaaatga tcttcctcac aatcttatct tcttttttac
     2761 gcagatttct ccgcttgtcg attatatctg ctgtagctgc cttactggct tcttttttat
     2821 ctttacttgg gtgttgtttg tttcctgaca aaacaactcc tccttactat catcttcgta
     2881 actctgttta ttatacatga aatttctact gccgtgaatt attttaccaa atattatgtc
     2941 aaaattgtaa gtggcaaata ctctttttga ggattacaat gaaaaatagt taaaaatgga
     3001 tagaaaggaa ttaacatgtc aaaaggaaaa catcgaaaaa aacaaaaaat ttctatttct
     3061 tttcttgtaa ttttattatt gtctgtcagc ttcttttcaa caggcatcat tataggaggt
     3121 caattaacaa aaatacctca ttcccaaact tataaagcgg agccaacaca acttacttcc
     3181 aatttaacgc ccgatgagca gaggcttgct agcaaaattg atcaattact acaaaaatca
     3241 gattatatcg gttcgattta tgttcgcaaa aataatcgcg tactattaga aaaaggatat
     3301 ggatatgcaa atctgcgcgt aaaagaatcg aattcacctt ctatttacta tcaaatcggt
     3361 tcgattcaaa aagcaatgac tgctttactg atactaaaac aaatccaagc tggtaagtta
     3421 acttttgaca caaagttagc agaattttat cctcagattc ctggaagtca gcaaatcaca
     3481 atcaaaaata tgctttatat gcgctctggt cttcatcgaa ctgcttctcc cgacaaacct
     3541 atgtcggata gtgaaatcat tcagttcgcc cttcatcatt tgaaacttac tgattatgat
     3601 acgtatcatt atgaaccatt gaattataca ttgcttacag ggatattgat caaactaacg
     3661 aatcagcctt atgaaactct tctaaaaaaa gaaatcattc gtccactcca tctagaacat
     3721 acagattttt atgacaatgt aaaaaattcg cctcaacatg ctgtatctta ccagatgtct
     3781 gcaactgacg attattcaaa agcattgatc gagcctgaaa ccgacatccg aaatgaatta
     3841 ggtacaggaa acataagtat gtctgtttat gacttgaaca aattctttac cagtgttctt
     3901 tctggcggta tcataccaaa agaactactt ttctcattgt ggcaaaataa tggtgataaa
     3961 catccttaca gcggcggtgt ttacagtgga aacgattata tccttgctca ggggaatatc
     4021 aatcgattcc atgcagtagt ggcgatgaaa aaagacacta aagatgcaat cgtcatggaa
     4081 agtaatatcc aatcggataa gaacatcaaa ttgccggcca ctgatttaag aaatcagatt
     4141 tatgaactga tggaaggtgt tcacttatca aattaaattt tgacataata ttagttgtca
     4201 tctctgtata gtcaatttag ttagtactct atctgaaaag tagaaataa
//
LOCUS       DAXWLG010000188         4197 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXWLG010000188 DAXWLG010000000
VERSION     DAXWLG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.193"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(63..200)
                     /locus_tag="ACGE4T_002482"
     CDS             complement(63..200)
                     /locus_tag="ACGE4T_002482"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387693.1"
                     /translation="MGYVIENFEYKLSRLPKKKGATQKELANKLGVNVQSVSNVEEDK
                     S"
     gene            complement(233..430)
                     /locus_tag="ACGE4T_002483"
     CDS             complement(233..430)
                     /locus_tag="ACGE4T_002483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387694.1"
                     /translation="MGQYFLTGVWYVLKLVIILGIAYICFRILLSGEGMPTPKDFIIS
                     VLNFVADQIQVIIHWAQQRFG"
     gene            complement(935..2110)
                     /locus_tag="ACGE4T_002484"
     CDS             complement(935..2110)
                     /locus_tag="ACGE4T_002484"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8387695.1"
                     /translation="MDGTQLKQMRLSRNLTQKLVADTLGWPQSFLSMIENDQRSCSKE
                     QEEALCEFYANFSSFTSLEIKFDYVRVRVPTNDTKTVVEEILGMDFNSFFYAETRLYG
                     YIEMYQKGMIRVLNSKKGDDKGVLIELSGQGCRNYESVLDERNDSWYQFFTRCVSFNG
                     VPRRIDIAVDDFEELFSLHEFAQKLNRGEFDSKFRTWRFQEEKQLLDNLSSGLSVYLG
                     SSQSLMYFCFYQKNYEIAKKQKLPLEEVEVKNRYEIRLRDAKAVSFIEQYLNKVDFSD
                     LTMGILANQLIMFEGEKNANVWKPWGKLIQNVGKVNLTITPEKPNYNRKLMYLKTQPA
                     RTMKIVKTVDEILGLNRFGEILEETELREQDEKIVEDEVRAVYEYIQENPDAIHYLL"
     gene            complement(2649..2813)
                     /locus_tag="ACGE4T_002485"
     CDS             complement(2649..2813)
                     /locus_tag="ACGE4T_002485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387696.1"
                     /translation="MYEEIPATCGTRLEIKDLMIDSFLAIVFSEKRLSFFINKKVENR
                     WRLERMYPTS"
     gene            complement(2806..4125)
                     /locus_tag="ACGE4T_002486"
     CDS             complement(2806..4125)
                     /locus_tag="ACGE4T_002486"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8387697.1"
                     /translation="MFQYKGIRIRNRHKYASERFIFYLMLPVLLLMSGFFSLFYTTIT
                     WEWCCIYWIIAFGLAGLFAYMCLYFYFYNVYQRLVHKGKLAKMIHSRRLILERVLKNG
                     RRKVVYYPKIFYHYKNGLIHVRLPLDLGPFQDRFNEMAGQLEEAFFCDLVEEQREKNY
                     ICYTFLYDVEKNRIGIRDITVKDGIITLMKNVQWAFNKAPHAIIIGSTGGGKTYFIMS
                     LIYALLPYGTFDICDPKRADLASLEKIPIFKDQVFYGGGILSCLINLAKETANRYDFL
                     REQPEYELGYDYTYYHLTPHFLVIDEWAAYYASLDTKEKKKAMGCLNQIALMGRQCGV
                     FLILGTQRPDTKYLDGAIRDNFGLRVTLGRVSKAGYKMIFEHTVKSLFNKPIKGRGYI
                     DSGTDQVREFYAPFIDITRFNFFAEFKQLSDQLMAEKIKETKEGKNV"
BASE COUNT         1352 a          883 c          526 g         1436 t
ORIGIN      
        1 aataattcaa tcgatgaagc atcaaagaaa gaaactgttt ttttctaaat tactaaatgt
       61 agttagctct tatcttcctc aacattagaa acgctttgta cattgacacc taatttatta
      121 gccagctctt tttgtgtagc tccctttttc tttggcaatc tagataactt atactcgaaa
      181 ttttctatca catagcccac ttctaaacat taaatttttt aaatatattt ttttatccaa
      241 aacgttgctg cgcccaatga attatcactt gtatttgatc cgccacaaaa ttcaacacgc
      301 ttatgataaa atcttttggc gttggcattc cttctcccga taataaaatt ctaaaacaaa
      361 tataggcaat acctaaaata atcactaatt tcaatacata ccaaacaccg gtaagaaaat
      421 actgtcccat ttttccaaca tattcctttc tttaaagtgt taataaccaa ataatactac
      481 agacaataca aactaaaaga aacagtaatt tcaaaaaatg ccaacctaca gaaaataaaa
      541 atcgaatcat ttcttttccc ctctattttt catatataga tacagttttt cttccaatac
      601 ttgttgtaaa tctaggatat cttgcttggc aaatcgtcat atttcatcat tgtttttact
      661 gctttttcgt atatccacat acttgtgttg catataaata aatctttgtc aacgaatatt
      721 ttaacgaatc actaatacgg tagcttaata cttcccctat ttctacatgt aacattttga
      781 ttgattgttg ataatctaaa tattctttaa ataactgtag atcatttaca acacatagga
      841 tcgtttcttt tattaattgt taacaagcaa tcatatactc tccccatcat tggtcttctg
      901 ttttaatcat gtttccccca tcttatcgca agctttatag caaataatga atcgcgtctg
      961 gattttcttg tatatattca tatacagcac gcacctcatc ttctacaatt ttttcatctt
     1021 gttcccgtaa ttctgtttct tctaaaattt ctccaaatcg attcaatcct aaaatttcat
     1081 ccacagtttt tacaatcttc attgtacgag caggttgtgt ctttaaatac atcaatttcc
     1141 gattataatt tggcttttct ggtgttattg tcaaattgac ttttccaacg ttctgaatca
     1201 attttcccca tggtttccac acatttgcgt ttttctctcc ctcaaacatt attaactgat
     1261 tggctaaaat ccccatcgtt aaatctgaaa aatctacttt atttaagtat tgttcaataa
     1321 aactgaccgc ttttgcatca cgtaagcgaa tttcataacg attttttacc tcgacctctt
     1381 ccaacggaag tttctgcttt tttgcaattt cataattctt ctgataaaaa caaaaataca
     1441 tcaaactttg tgaactacct aaataaacag ataagccact acttagatta tctaacaact
     1501 gtttttcttc ttgaaaccgc caggttcgaa attttgaatc aaattcgcca cgatttagtt
     1561 tttgtgcaaa ttcatgtaaa gaaaatagtt cttcaaaatc atctaccgca atatcaattc
     1621 ttcgaggcac accattgaaa gagacacatc gtgtaaagaa ttgataccaa ctatcatttc
     1681 gttcatccag tacactttca taattccgac agccttgacc agataattcg atcaacactc
     1741 ctttatcgtc acctttttta gaatttaata cacggatcat gcctttttga tacatttcaa
     1801 tataaccata taagcgggtt tctgcataaa aaaaagagtt aaaatccatt cccaaaattt
     1861 cttccacaac tgttttcgta tcatttgtcg gaactcgtac acggacataa tcaaatttaa
     1921 tttctaaact cgtaaaactt gaaaaattcg cataaaattc acaaagtgct tcttcttgtt
     1981 ctttcgagca acttcgttgg tcattttcta tcattgataa aaaagattgt ggccatccta
     2041 atgtatctgc cactaatttc tgcgtcaaat tccgacttaa ccgcatttgc tttaattgtg
     2101 tcccatccaa atatatcttt cctttctttt gtgatatttt atgttacaaa gcgaagaaaa
     2161 atatcacaaa atttttgaag caaaatagtg ttatatcaat gtttcaatca cccatctttg
     2221 ccaccgaata taaattctac ccccctgtta gaatatgggg gttttaggcg tgcgctacgc
     2281 gcacgcccca caacgcaccc tgcacctcag ggctttcgct aacgctagga cgcttccgcg
     2341 tccttccgcc ctgaggcttg tttgcttgtg gagcacacgc acagcatctt ccgtattcta
     2401 atgaggacaa atttaatttt ctgacaacgc aaaggacaag ttaatatcct ctacttgtag
     2461 gtacagtcga gaccggtctt tacttttaac ataagaacag gaaagtcctt tgaatcgtcc
     2521 ataaaaaaca ggctgttccg ctgctttttc gacaatttgg tcactatagg atcgagcact
     2581 tttatttgaa gtaaaaaccc gtcatattcg ccttcgataa ttgtcataag gcaactgtaa
     2641 ccaatcagtt aactcgttgg atacatacgt tctaatctcc aacgattctc tactttttta
     2701 ttgataaaga agcttaatct cttctcactg aaaacaattg ccaaaaaaga gtcaatcatt
     2761 aaatctttta tttctaatcg agttccacat gtagctggga tttcttcata cattttttcc
     2821 ctctttcgtc tccttgattt tttcagccat taattgatca gatagttgtt taaattccgc
     2881 aaaaaagtta aatcttgtta tatcgataaa tggcgcataa aattctcgta cttgatcggt
     2941 tcccgaatca atataaccac gacctttaat cggcttatta aataatgatt ttactgtatg
     3001 ttcaaagatc atcttatacc cagctttgga tactcttccc agtgttacac gtaaaccaaa
     3061 attatctcgt atcgcgccgt ccaaatactt tgtatctgga cgttgggtac ctaaaattaa
     3121 aaacacgcca cattgacgac ccattaaagc aatttggttc aaacatccca ttgccttctt
     3181 tttttcttta gtatctaacg atgcataata tgctgcccat tcatcaatca ccaaaaaatg
     3241 aggagttaga tgataataag tataatcgta ccctaattca tactcgggct gttctcttaa
     3301 aaaatcgtat cgattagcag tttctttagc tagatttatt aaacaagata aaataccacc
     3361 accataaaaa acttgatctt taaaaattgg tattttttct aaactagcca aatctgcacg
     3421 ttttggatca caaatatcaa acgtaccata cggtaataat gcatagatca aagacattat
     3481 aaaatacgtt ttcccaccgc cagtacttcc tataattatg gcatgtggtg ctttattaaa
     3541 tgcccattga acattcttca ttaaggtgat tatgccatct tttacggtta tatctcgaat
     3601 cccaatacga tttttttcca catcgtataa aaaggtataa caaatatagt ttttctctcg
     3661 ttgctcttct actaaatcac aaaaaaaggc ttcttctagt tgcccagcca tctcattaaa
     3721 ccgatcttga aagggaccta aatctaatgg caatcgaaca tgaattaagc catttttata
     3781 gtgataaaaa atttttggat aatacaccac tttccgccgt ccatttttca atactctttc
     3841 aagaatcaat cgacgactat gtatcatctt tgctaatttt cctttatgaa ccaatcgctg
     3901 ataaacatta taaaagtaaa aatacaaaca catatacgca aatagaccag ctaaaccaaa
     3961 tgcgattatc caataaatac agcaccattc ccatgtgatc gttgtataaa agagagaaaa
     4021 aaatccactc attagcaaca gaaccggcaa cataagataa aaaataaatc gttctgaagc
     4081 atatttatgt cgatttcgaa tacgaatacc tttatattga aacacttatt ctcatctcct
     4141 cgcatcatag agtgactaat gtattggata aaagaaaacc ttcattaaca ctaacaa
//
LOCUS       DAXWLG010000189         4081 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXWLG010000189 DAXWLG010000000
VERSION     DAXWLG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.194"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(48..527)
                     /locus_tag="ACGE4T_002487"
     CDS             complement(48..527)
                     /locus_tag="ACGE4T_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8387698.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            886..1668
                     /gene="rpsB"
                     /locus_tag="ACGE4T_002488"
     CDS             886..1668
                     /gene="rpsB"
                     /locus_tag="ACGE4T_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFF8387699.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1767..2648
                     /gene="tsf"
                     /locus_tag="ACGE4T_002489"
     CDS             1767..2648
                     /gene="tsf"
                     /locus_tag="ACGE4T_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFF8387700.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2784..3506
                     /gene="pyrH"
                     /locus_tag="ACGE4T_002490"
     CDS             2784..3506
                     /gene="pyrH"
                     /locus_tag="ACGE4T_002490"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFF8387701.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3509..4066
                     /gene="frr"
                     /locus_tag="ACGE4T_002491"
     CDS             3509..4066
                     /gene="frr"
                     /locus_tag="ACGE4T_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFF8387702.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1460 a          720 c          883 g         1018 t
ORIGIN      
        1 ttgaaaataa aaaaacacgc attccatatt aggaatacgt gctctgatta aaaaagtgat
       61 acttcagttg gatggtgaag aaattcttct actcgttttg cgtcctcctc attttccgag
      121 atgaccatga tcgtatcgaa tccagcaatt gtactgacaa tagagggagg tttgatctca
      181 tctaaaacag atgcaaataa atgagcattg tctggcaagg tattgacgat cgtcaaaaag
      241 tggactcgtt cgactttcaa tacaatgtct tcaatcattt gaaccagccg atcctcttcc
      301 tcagtcctag tctcaggagt tcctttttcg cgaaacaata cgaagcggga aacgccattt
      361 tcatcaggtg ccttcacgat ttttagatca cggatatctc gagaaatcgt tgcctgcgtg
      421 gcggtaacgc cataatgccc aagtttgttt aatagttctt cttgcgtacg gatcgggaat
      481 tcttcaatga cttgtttaat cagcaaatgt ctttgagctt tgcgcatttt ctttctcctt
      541 tacgaaagat ttcacatgta tcataacaaa aaaaagaagg attgcccaat aatattcgtc
      601 ttttatacct aaaattattt tatggaaaaa ggataataaa gaaacaaaga gaaatctact
      661 gtttttcttg aattcttttt atctttcagg taaactattg aatgttgagc agaaaagtat
      721 atttgctctt gcgatacgga tcttttgtct gtataatagc tgagggtgct ttatgcatca
      781 gcaaatccac attcttattg gattgtcggt aggtgcttga aaaagtttgc cggcaaggta
      841 gaagataaga agaggaaaaa taaaaccaaa tcggaggaaa caaacatggc agtaatttct
      901 atgaaacaat tactagaagc cggcgtacac tttggtcacc aaactcgtcg ctggaatcca
      961 aaaatgaaga aatatatctt cacagaaaga aacggaatct acatcattga cttgcaaaaa
     1021 acagttaaat tagtagatgc agcttatgat tacatgaaga gcgttgctga agaaggcggc
     1081 gtagcattat tcgtaggtac aaaaaaacaa gcacaagaag caatcaaaga agaagcaact
     1141 cgtgcaggac aatacttcgt taaccatcgt tggttaggcg gaacattgac aaactgggat
     1201 actatccaaa aacgtatcag ccgtttgaaa caaatcaatg caatggaaga agatggaact
     1261 tttgaagttc tacctaaaaa agaagttgct ggtttgaaca aagaacgtga acgtcttgaa
     1321 aaattcttag gcggtatcgc tgatatgcca agaatcccag atgtaatgta catcgttgac
     1381 ccacgtaaag aacgtattgc tgttcaagaa gcacacaaat taaatatccc aatcgtagct
     1441 atggttgata caaactgcga tccagatgaa atcgacgtag taatcccttc aaacgacgat
     1501 gcaattcgtg ccgttaaatt gattacttca aaaatggctg atgctttcat cgaaggaaac
     1561 caaggtgaag atcaagtagt tgaagaagat ttcacagaag aaaacacagc aacatcaatc
     1621 gaagaaatcg tagatgttgt tgaaggcgac aattcttcag cagaataatt ttgaatgatg
     1681 gagctgtttc aaaggctggg cgacaagcct cactctcctt tgaagcagct ttttttaaaa
     1741 agaaaaacac acaggaggag ttaaacatgg cagacgttac agctaaaatg gtaaaagaac
     1801 tacgcgaaat gactggcgta ggaatgatgg atgcgaaaaa agcattagtt gaagtagaag
     1861 gcgacatgga aaaagcagtt gatcttttac gtgaaaaagg aatggcaaag gctgcgaaga
     1921 aaaatgaccg cattgcagct gaaggattgg cttcagttgc tgtgaaaggt aacactgcag
     1981 caatcgttga agtaaactca gaaacagact ttgtttctaa aaacgaaatg ttccaagatt
     2041 tagtaaaaga aattgcagaa cttgttgctg aaaacaaacc agcagacatg gaagctgcaa
     2101 tgaaaatcaa aacagataaa ggtacaattg aatctgactt gatcgaagca actcaagtta
     2161 tcggagaaaa aatcagcttc cgtcgctttg aagttgtaga aaaagacgac aatgctgcat
     2221 ttggtggata cttgcatatg ggcggacgta ttgccgtatt gactgttcta gatggaacaa
     2281 ctgacgaaac agttgcaaga gacgtagcta tgcacgtagc agctatcaat cctcgttatg
     2341 taaacgaatc acaaattcca gaagctgaat tagaacatga aaaaactgtt cttacagaac
     2401 aagcattgaa cgaaggcaaa ccagcgaata tcgttgaaaa aatggtagaa ggacgcttga
     2461 agaaattcaa agcagaaatc gctttggtgg accaaccatt cgttaaagat cctgatatga
     2521 ctgttgaaaa atacgttgca tcaaaaggcg caactgttaa aacattcgta cgtttcgaag
     2581 ttggcgaagg tatcgaaaaa cgcgaagata actttgtaga agaagttatg agccaagtga
     2641 aaaaataatt ctgttgaata acagacggga acgcacggtt tgtgcgctcc ctttttttaa
     2701 gcaaaacaag aaaaaccagt cttagagtgt cgaaatcatc taagtatggt aaaatggcaa
     2761 tagaaaatat tggaggaatg aacatggtca caccgaaata tcagcgtgta gtcttaaaat
     2821 tgagtggaga agctttagct ggggatgcag gatttggaat caaaccacca gtaatcaaag
     2881 aaatcatcca agaaatcaaa gaagttcacg agctgggcgt tgaaatggca atcgttgtgg
     2941 gtggcggaaa tatctggcgc ggacaaatcg gagcacaaat gggcatggaa cgtgctcaag
     3001 ctgattacat gggtatgtta gctacagtta tgaatgcctt agctttacag gatacgttgg
     3061 aaaacgtggg tgtaccgaca cgcgtgcaaa cttcgattga aatgcgtcaa atcgcagagc
     3121 catatattcg ccgtaaagca gaacgtcatt tagaaaaagg acggatcgtt atctttgcag
     3181 gcggtaccgg caacccgtac ttctcgacag acacaacagc agcattacga gcggcagaaa
     3241 tcggcgcaga tgttatcctg atggctaaaa acaatgtaga cggtgtttat tctgctgatc
     3301 cgaaagtaga tgcaaatgca gtaaaatttg aagaactgac tcatctggaa gttattgcca
     3361 aaggattaca agtcatggat tcaactgcaa gttcactaag catggataat gatattccac
     3421 tgcttgtctt caatttaaat gaacacggaa atattcgccg tgcaatctta ggcgaaaata
     3481 tcggaacaac tgtaaggggg aaataaaaat ggcagatgca attatgacag aagcaaaaga
     3541 aaaaatgaaa aaagcagcac aaaatcttca acgtgaactt gggcaaatcc gcgcaggccg
     3601 tgcgaacgct agccttttgg accgtattac tgtgaactac tacggtgcgc caacaccatt
     3661 gaaccaaatg gcatcaatcc aaattccaga agcacgtgtt ttaatgatca caccttttga
     3721 caagagcatt cttcaagatg ttgaaaaagc tattatggca agtgatatcg gaatcagtcc
     3781 aaccaatgat ggaaatgtca ttcgcttagt tatcccgcaa ctgacagaag aacgcagaaa
     3841 agaattagct aaagatgtga aaaaagaagc agaaaatgca aaaatcgcga ttcgtaacat
     3901 ccgtcgtgat gcgatcgatg aatataaaaa acaacaaaaa aatggtgata ttaccgaaga
     3961 tgatttgcgc ggattagaaa aagacgtaca aacattgaca gataacagca ttaaagaagt
     4021 agataagatc gctgctgata aagaaaaaga attattggaa gtttaataaa atcataaaaa
     4081 c
//
LOCUS       DAXWLG010000190         4079 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXWLG010000190 DAXWLG010000000
VERSION     DAXWLG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.195"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(188..1693)
                     /locus_tag="ACGE4T_002492"
     CDS             complement(188..1693)
                     /locus_tag="ACGE4T_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8387703.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1690..2376)
                     /locus_tag="ACGE4T_002493"
     CDS             complement(1690..2376)
                     /locus_tag="ACGE4T_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8387704.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2569..3990)
                     /gene="gndA"
                     /locus_tag="ACGE4T_002494"
     CDS             complement(2569..3990)
                     /gene="gndA"
                     /locus_tag="ACGE4T_002494"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFF8387705.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1124 a          861 c          695 g         1399 t
ORIGIN      
        1 acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact tacatttaat
       61 taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat ccctgaattt
      121 ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa caagtaaagc
      181 acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa attgtccctt
      241 gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt tgtttagcga
      301 tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc actcgataaa
      361 aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag tcctgaatcg
      421 cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt cgtgtagttg
      481 aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga ttacgataaa
      541 tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag tctggatata
      601 atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt gtttcattcg
      661 cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga actaagcttt
      721 tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc tcaggatcat
      781 ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca ggtgtcctta
      841 actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa cgcatacggt
      901 caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg tcgatttcag
      961 gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct ctgagtacct
     1021 tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt aagatcaaag
     1081 aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca tagaaagaag
     1141 ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta gaatatatag
     1201 gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga actttacgtg
     1261 tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc tcgtccaaat
     1321 cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa tcgatcttca
     1381 ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca tttgtcgctt
     1441 tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta gccaaacgag
     1501 aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca atcagattga
     1561 tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg aagaaagaac
     1621 tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt tccgattttt
     1681 tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga tatagctttc
     1741 ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca ctacgtttgt
     1801 ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg ctaataccac
     1861 gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta gttcaattac
     1921 ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc gataagtaat
     1981 agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca acaatgcacg
     2041 aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat ctgctccgtg
     2101 gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa tgattggtgt
     2161 atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg ggagcatcag
     2221 atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag ttcggccgtt
     2281 gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa atcgtgccaa
     2341 gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc tttctattct
     2401 actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc tatagtcatt
     2461 ttatcatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat cgacttcagc
     2521 tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt attcttcatg
     2581 gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat gtgcgccaaa
     2641 gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt atgaatcgaa
     2701 gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag ccaaggcaac
     2761 gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat catctagcaa
     2821 taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca agaatcgagc
     2881 tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca agtcccaatc
     2941 aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca tgattttact
     3001 gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg tgaactcaaa
     3061 atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat aagcagaaat
     3121 atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt caagcgcgct
     3181 ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca catctactac
     3241 tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga tttctatcaa
     3301 atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt cttctactga
     3361 aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca tatctccata
     3421 ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc caatatatgt
     3481 gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg gtgcaactag
     3541 cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag ctccttcttc
     3601 accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact cttcattacg
     3661 acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc ctttatcaag
     3721 gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga ccattagcaa
     3781 gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg ttgctttgaa
     3841 atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac gattgaataa
     3901 agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca taacggccat
     3961 accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg ggatttctcc
     4021 ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac aacatattt
//
LOCUS       DAXWLG010000191         4034 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXWLG010000191 DAXWLG010000000
VERSION     DAXWLG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.196"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            27..1451
                     /locus_tag="ACGE4T_002495"
     CDS             27..1451
                     /locus_tag="ACGE4T_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFF8387706.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1632..2405)
                     /locus_tag="ACGE4T_002496"
     CDS             complement(1632..2405)
                     /locus_tag="ACGE4T_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8387707.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2402..3307)
                     /locus_tag="ACGE4T_002497"
     CDS             complement(2402..3307)
                     /locus_tag="ACGE4T_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387708.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3461..3781)
                     /locus_tag="ACGE4T_002498"
     CDS             complement(3461..3781)
                     /locus_tag="ACGE4T_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFF8387709.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1205 a          749 c          814 g         1266 t
ORIGIN      
        1 gcggttttat tttggaggat gcttacatgg atacagagca acagaaaaaa ggaattggtt
       61 taattccact tgctgcctta gtaattggtt cagcgattgg aggtggcgta tttggtatta
      121 tgacggacat ctccggatcg gctggcggcc cagctatttt cagttgggta ctcgtaggaa
      181 cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt ccagatttaa
      241 aaggcgggat ctttagttac gcagaagaag gttttggccg tttcgctggt tttatcagcg
      301 gttggggtta ctggctcaca tgctggttag gcaacgtagc ttttgcaaca ttgttgatga
      361 gcgcattagg atatttcttc ccagtatttg aaggtgggca aaatctggca tcgatcgtga
      421 tgagtacagt ctttttatgg ggatatgcat ggctagtgaa tcggggagtc gaaaatgcta
      481 gtttcgtcaa tgctatcgtc acattctgta aattagtgcc attattttta tttattgtag
      541 tagctatcag cacgttttcg atccaacaat tcattgataa cttttctagc gacattatat
      601 tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt attgagttgc atcatggtga
      661 tgctttgggt atttgttggt attgaaggag cctctgtcgt aggttcacga gcaaaaaaac
      721 aatcagatgt tgggaaagca acgattctag ggatattcgg cttgattatt atttacgtct
      781 tggtttccat gcttccatat gggacaatga cgatgaatca gctgcaaacg atcaaggcgc
      841 agcctgctat gggctatgtg tttgaaatga tggttggtaa atggggagca gtcgtgatca
      901 acggcggttt gatcatttct ttagttggtg tttggctctc atggacgatc ttaccgattg
      961 aaacaatggg aaacatggct gaagacggac ttttgcctaa agcatgggga aaactcaaca
     1021 aaaatggggc accaacttat gctatcgtat tgacgacgtt atgtacgaat gtctttttac
     1081 tttcattatt attcactgac agtgcttata actttgctta cacacttggt acagcagcta
     1141 ttttcttcac gtggcttttc ttaggattgt accaaatgaa actttcttat cagcggaaag
     1201 aatgggtcca atttttagta gggcttttag caagtgcctt tcaagtatgg gctatgctag
     1261 tcgtagcctt taatgaagta atgcttgttc ttgttctttt cttgccaggc atttattttt
     1321 acctcaaagc acgtaaggag caaaacgagc ctgtacaaca tatccactta gataagattt
     1381 tacttatagg tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc actgggatgc
     1441 ttcacgtata gaagtataaa agaagtataa agagaggttg tgagcctgtc gtttattcaa
     1501 aaacatacta ggaaaggagc tgtgacacaa atgttgtcac agctcctttt tcagaataaa
     1561 cggtgtttaa gagacagcca atcggcaata aggctgaacg tctctttcac agcctctttt
     1621 ttgatgagtc atcatacatt ttttcgctta aatacgagat atcctaatgc taagaacaaa
     1681 acgatgtaag ctaaattacc gaatagcatt tgattagtag aaagatgagt caattgcttc
     1741 atgatctctt cgtttccaat ttggttttgc agattcaaca tactgatcgg attccatttg
     1801 atccactccc atttttggat gagtgcaata aggataccag atatgattga cgaagcaaaa
     1861 taaaagacga ttcctgctga gatggatgcg ccggaactat tgataaagca agcaatcatc
     1921 aacactaaac ttaaaatcag ccataaaccg atataactac ccaaagtata agtgaacaag
     1981 ctttggatca gtgtttgacc agtagaaacc ttctccgtga aggaaatcga agggaaaaga
     2041 atcagtttca tcaacacagt gacaataata gctaaaaggt ataaataaac agaataaata
     2101 actaatgtga tccatttgct tactaatatt tctcctcgag aatactttcg ataaaggaga
     2161 tttttcaatg taccatattg tgcttccatc gaaatgatcg tgcttgcagc tgcaatcatg
     2221 ataaagacaa tccaagatgt ggcactaaaa agctgagcag tcatttcaac tggatcaatg
     2281 atcgtcgtgt attttttggc tagaagtgct gttcctatga gcaaaacaac taatagaaca
     2341 gataaaacag cagttgactt tttcttgatc aatttaaata attcttgttt tacaagcaat
     2401 gtcatttttc ttcttccccc tttactgaat ccagaatttc caatagagaa gattccaaat
     2461 cattcgtcag atgctggacg tctgtcacat ggatattttg tgcagacaat agctggatca
     2521 cggttcctaa taattcttcg ttttctttta aaagcttgat tttatcagga tcttccaaag
     2581 tatatccagc ttctgtcaag atcgttcgag cagctagatc atttgttgta gagataacga
     2641 tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg atctttcctt
     2701 gattgatcag gatcagctca tcgaccattt tttcaagctc gctgagtaag tgactggaga
     2761 tcaagaaaga ggtgccctga gtagccaagt cggtaatgat ttttcgcaca tctcttgttg
     2821 cttgtggatc caaaccgttc atcggctcat ctaaaataac gatagacgga tggttcagta
     2881 aggcaagagc aattcccaat ttttgtttca ttcccaacga gtaggatttc gcttttttgt
     2941 gaatgtactt ttccatcttg agctgattca cgatagtgtc gatagcagat acatcgttgg
     3001 tttcagagaa taacttcaga tgatcatagc ctgttaagaa tggatagata cccgggtatt
     3061 cgatcaatgc tcctacttgt tccaaccctt tatgactaga gggagaaatc tcattttcac
     3121 caatacagat tgttccatct gagtaatgga tcagtcctaa aatagctttc atgatcgttg
     3181 tttttccagc gccattagga cctactaatc caacgatttt gccaggggat aagctaaaac
     3241 tgacatcgct gagggcttgt ttgtgaccaa agttcttgga taattcttgg accgaaagac
     3301 ttttcaaaaa tattcactcc ttttctatag atagtacaag catacacctg atgagtggag
     3361 aagtcgctag gtcattggac ttatttctta ttttttcata aaaaaattga atattaaata
     3421 aaaaggctta ttttttctga tcagtactta ttccaagtgt ctagtggccg ctagtaatct
     3481 tgatgccaat gatactgatg agtagcatcc ccacaaagaa ccaagtggaa agattcagct
     3541 tatcatgaaa caagaccaca ccaattatga ccgaaccaac tgcgccaatc cctgtccaga
     3601 taggataaga gagactcatc ggcaaacttt tgattgcctt tgacaaaaag taaaaactag
     3661 caatcatgcc aatcactgtg taaaaagaat aattgatttt agaaaaacca tcactccatt
     3721 tcatcatcgt tgaccagaat acttccaaaa ttcctgctat gattaattcc atccatgcca
     3781 tacttacatt cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc atcccttcaa
     3841 agacctaatg gaatgatcat cctctttatc ctacccggtg gcagattgta tatctttttc
     3901 tgtagtatag cacacctatc cagatatcgg caatctcata ctttttagta aagtgtacca
     3961 agcactgacg gatatatcgt tagcggaaaa gaagatgaat gctttgcaat ttcatagaaa
     4021 gaagttgtta ttta
//
LOCUS       DAXWLG010000192         4012 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXWLG010000192 DAXWLG010000000
VERSION     DAXWLG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.197"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            12..1361
                     /locus_tag="ACGE4T_002499"
     CDS             12..1361
                     /locus_tag="ACGE4T_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8387710.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1489..2079)
                     /locus_tag="ACGE4T_002500"
     CDS             complement(1489..2079)
                     /locus_tag="ACGE4T_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8387711.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2226..3578
                     /gene="brnQ"
                     /locus_tag="ACGE4T_002501"
     CDS             2226..3578
                     /gene="brnQ"
                     /locus_tag="ACGE4T_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8387712.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1138 a          747 c          789 g         1338 t
ORIGIN      
        1 atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg actgaaaaat agacatgtcc
       61 aactgatttc tattggcgga gcgattggaa cggggttgtt tttaggttcc ggaaagtcta
      121 tccagctagc tggcccttcg atattgctcg cctatcttat tactggatgc atctgttttt
      181 tcattatgcg tgcgttaggc gaacttctgt tatcaaacac caataaccat tcttttctgg
      241 attttgttgc agaatatctt ggcaaaaaag cggctttcat cactggctgg acgtattggt
      301 tctgctgggt atcgattgca atggccgatt taacagccat cggaatgtat gtacgctact
      361 gggcgcctgg tgttcctcag tggcttcctg aactgattgc attaggtctt cttctttgct
      421 tgaatatggt ggctgtctca ttgtttggtg agttagagtt ttggtttgca ttgatcaaag
      481 ttgtagcgat cattgccttt atcctcgtag gagcttatat gatcctcacc cactatacga
      541 cagatattgg cacagccagt atcacgaatc tatggtctca tggcggattc tttccaactg
      601 gtgcaaaagg atttatctta gctttccaaa tggtaacctt tgcttttgct gggatagaat
      661 tagttggatt agtcgctgga gaaacagaaa atcctgaaaa agtgttgccg gaagctatca
      721 ataatattcc tatcaggatc atcttatttt acttaggatc tctttttgtc attatggcaa
      781 tctatccatg gaacagcttg aatccagata acagtccatt tgtagaagta ttttcggaaa
      841 ttgggatcac gattgctgct tccttgatca atttagtcgt attaagtgca gcagcttctg
      901 cctgcaacag tgcaatatat agtacaggac gtatgcttcg ttctttagcg caagaaggca
      961 gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt gccagggaat gctttgacat
     1021 tttctactat cgtcattttc atttctgtta tcttgaatta cgtgatgcct agtgaagtat
     1081 tcacactagt aagcagtatt gccactactt gtttcttgtt tatctggagt atgcttgtct
     1141 acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa agctcattca tttaaaatgc
     1201 cgttgtaccc atttagtaat tatctggtat ttctttacat ggcctttgtg tgtgtcgtct
     1261 tgttcctagg aaaagatacg agaatcgctt tattactaac acctgtgtgg ttcttgcttc
     1321 tattactaat ctatcatatg aaatatgaac gtaagaagta agaaagtagt tgtgacagaa
     1381 gtggtcagct tcgagaaata agaaggaatt tacgaaaatt gcttcttaaa tttttgtgaa
     1441 atttcggctt atttcttgga gttaaaagct tctgtcccag cctcttttct atttccccgg
     1501 aaacaaccgc tgataatagg catctgcttt tttataggaa aaaatccaaa taccagatac
     1561 aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag acaccggcaa tcaagcataa
     1621 gttaatcatc atggagataa gacttcgacg gtcctttgac aaaactctca cgacatggga
     1681 atctggttta tttgctttca tgatttgcct catcaagata ccaaaagaaa aactccacat
     1741 cgtgtatact aagacataag ccatctcagg ccaaaaagct tcagggttac gcccgaccca
     1801 actagtaaca gaaggacata aagtcagcca aaagagcaaa aagatattcg accagaccac
     1861 tcggtagtta attcgttctg ccatctgaaa tatacgatga tggttcaccc agatcaccgc
     1921 aacaaatata aagctgatgg tataagctaa taatgtattt cggatgaccc ataaagaatg
     1981 aaagctagat gtattaggga tcttgatctc taatacaaga actctcaaaa caatggctac
     2041 cactccatca gtaaatgctt ctaatctcga ctttgtcacg atgattcctc ctcagatgat
     2101 tcagcgcttt tattttatca cttacgttat taaataaccg tcgttttata taggaaattt
     2161 tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt taaaaaaacc gtaaaggagt
     2221 tatgtatgac aaaaaaatta tcatttaaag actatctttt tataggctcc atgcttttcg
     2281 gtctattttt tggtgctgga aatcttatct ttcccgttca tttgggtcaa gaagcaggcg
     2341 ctgctacctt ttgggcgaat ctaggtttct tggttaccgg tatcggtctt cccttcttag
     2401 gggtgattgc cattggtgta tcgaaaagct caggtgtata tgaattagca aaacggataa
     2461 atcaaacata tgcgatggtt tttaccatct tgctttactt agttatcgga ccgttttttg
     2521 ctttacctcg ccttgcaacc acatctttcg agattggttt agcaccattt atcgatccta
     2581 gcagacagac gctatatcta gcaattttca gtattgtatt ttttgtgctg gcttggtggt
     2641 tttcaagaaa accgacaaag atattagatt atgttggaaa gttcctgaat cctgcatttt
     2701 tggttttatt aggtgctttg cttctccttg cttttttgaa tccactaggt tctgtaaatc
     2761 aagcgcctat tcagccaaat tatcaagagc atgctttttt cacagggttt actcaaggat
     2821 ataacacatt agatgcgctt gctgctttag catttgggat catcattgtc acaacaatcc
     2881 aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga cacgatcaaa tctggtgcga
     2941 tcagcattat attgatggga atcatttata ctttgctcgc ttatttaggt gctatgagct
     3001 taggtagctt tgctctaagt gaaaatggcg ggatcacgct ggctcagatt gctgatcatt
     3061 atttaggtac ttatggcagt atcttattag catttatcgt tatattagct tgcttgaaga
     3121 ccggtattgg tttgattaca gcttttgcag aaacattcac agatttgttt ccgcaaagaa
     3181 attacgcatt ctttgttgca cttgctagta tccttccttg tttagcggca aacgtaggct
     3241 tgacgaatat cattcaattt tcgttgcctg ttttgatgtt catctatcca ttagcaatga
     3301 cactgatttt actagtgtta gttggtcctt tgtttaagca gcgtacggct gtttatcgaa
     3361 tgactactta ttttacattg attgcttcga tttttgatgg gttgaacgct tgcccagaaa
     3421 ctatcaaaca aacacctatt gttcaaaatc ttttatatgt tgcggaaagc tacttgccgt
     3481 ttttcaaatt ggggatgggg tggatcgtac cagcagttat cggttttgtt atcggactga
     3541 tttggagttt cgctaagaaa gaagaagtag cagattgata aaagaggttg tgaaagagac
     3601 gttcagcctt attgccgata ggctgtctct tgaacactgt ttattgagag gtcgtgaaaa
     3661 agccgctcag tttcgagtat taagaaatat tttttgaaaa tggcttctca catttttgca
     3721 taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag aggtcgtgaa
     3781 aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct cacatttttg
     3841 cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg agcgtctata
     3901 aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact ttgccgtttt
     3961 gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata at
//
LOCUS       DAXWLG010000193         3991 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXWLG010000193 DAXWLG010000000
VERSION     DAXWLG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.198"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(422..595)
                     /locus_tag="ACGE4T_002502"
     CDS             complement(422..595)
                     /locus_tag="ACGE4T_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387713.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(598..996)
                     /locus_tag="ACGE4T_002503"
     CDS             complement(598..996)
                     /locus_tag="ACGE4T_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387714.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1212..2267
                     /locus_tag="ACGE4T_002504"
     CDS             1212..2267
                     /locus_tag="ACGE4T_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387715.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2287..2640
                     /locus_tag="ACGE4T_002505"
     CDS             2287..2640
                     /locus_tag="ACGE4T_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387716.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2822..3109)
                     /locus_tag="ACGE4T_002506"
     CDS             complement(2822..3109)
                     /locus_tag="ACGE4T_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387717.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3122..3940)
                     /locus_tag="ACGE4T_002507"
     CDS             complement(3122..3940)
                     /locus_tag="ACGE4T_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8387718.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
BASE COUNT         1362 a          706 c          570 g         1353 t
ORIGIN      
        1 aaaagctaaa tattctattt ttgttatttt attagcaggg aaatttaaat agtttacaag
       61 aatccgtaga aatatattgg ttttgccttc agattaatac tggatcatat taatctgaat
      121 ataatatatg aactaataaa tagcttaacc tcgctatttt gttttttctg atcactatct
      181 atacagcatt agtaaataga attttaaaaa taaaaaaatt aagacaaaaa aagtatcaac
      241 ataaacatat acatatacac gtttatgttg atacacttaa tttgtccttt aaaaagcact
      301 tattttaata ttatgttatt ttataatctt attacaagct gattgttaga cgttgtcctt
      361 tcattacatt tattaacggt tattttatcg tttaatttat taattatttt tgatctaaaa
      421 cttagcttaa caaataaata attaaccaac cgccagataa aaatgcgatc attgctaata
      481 cataagcaaa gataaaaata tgttcgatca gcttaaaccc tgatgcgatt aagcagacca
      541 agtaaaatag aaattctctt acagataatg gtaattttct aactagcata ctcataatta
      601 acaccccaaa gtaatattgt attttgaaca ttttacatcc aatacagcat caatcaacca
      661 ttgccaatca aaaatacaag gcttcattga agtatgtttt tcagatgaaa agatataata
      721 taatttcttc ggaataatat tttcagttgt tgggatcaag ctaaatttaa taccatctaa
      781 ctcatagcta ccagctttct ctccattaaa aatttcctta gcactttctt ctaaagaatc
      841 aaaatatttt tcgacagcaa cctttgaagc ttttactatt gaagcatttc ccttttcgat
      901 aaaatctgct tttgttttac cgacaataag taattcttga tcagtaaaat cttctaaaat
      961 aatagtagtg taattcttat ttcttctcaa tcgcatcagg atcactcctt aatataattt
     1021 atgcttttat aatatcataa taagataaat ttgcaagtgt ttatttacac ctttttattt
     1081 acactttatt gtaattgatt atactttttt gattgcattt ttttcttata aacagttgca
     1141 actattagta aataggtgta aactattttc gtaccgaaat accataaaca caaacagaaa
     1201 gaggtcatac catgaaagaa ccattattgt tttccgttga cttagggaat aaatcagtaa
     1261 aagaaatgag tacacacgtt tctccactct cgcttccctc tcgtattttg aacaaaaata
     1321 tcattaattc aggacgtttt tctaatatag gttcctcatt aaaacgatca ctaaacgttt
     1381 ctgaatatag cctagtaaac actactgaaa gctattattt tgggaaggat atacacgagc
     1441 taggaaaaga tgactacgtg atagaatcac taggttttgg gatcagcaga tacaagcagg
     1501 aaacctacca agatatccta tctttctctt tagctaacct agcacgttta gaagatcaat
     1561 ctgatgacgt tttaaccgtt aatctagttt taggtctgcc ttcatccgac tataccgaaa
     1621 caatcgtcaa atacgtgatg caacaagcca cacgacaaca ttcagttgaa gttgacggta
     1681 aagcttataa tataaacgta gataccgttt gggtactgcc acagccgatt ggaacactat
     1741 ataacctcct ccttgctgat gatggtagtg tacaagatga atcattgatc agtcagaaga
     1801 tcggttttgt tgatattggt ggtggcacga aactttttga tgttgtccaa aattttgaaa
     1861 ttgacgaaaa caaccgtgaa caaaaggata ccggtatttt tactttatat aatcgtatcg
     1921 taaatatgct tgaccttccg atcagtccta atgctttcca acttgaaaag attatccgag
     1981 aaggcaaaga aaaaggcgta tatctattta aaccaagcgg aaatgaaagt tttgatattt
     2041 cagaagtagt tgaaagagaa ataaaaacat acaccaatat ggttgttgca gaagttttag
     2101 ctacattcaa aaacttcaca accttagata aagttgtctt tactggtggt ggggcttcta
     2161 ttatcgacaa acaacttgta aaagataaaa ttccgcatac cttgtttgtt gaagacagtg
     2221 aattttctaa tgtaaaaggc ttttacaagt ttggtttaac tcaataaaac agcgaaggag
     2281 atcaacatga ataagggaaa atcagagcgt tatgtagtta taatcaatag agaaaaggtt
     2341 agcgagttac ctttagcaga gtttcttgaa tctgaatatg aaacactagg cgttaaatac
     2401 tctcagacga tcaaaaaagc tttgaatcta tatcggcgtg taaagaacaa tgaagttaca
     2461 attattgaag caggcaacga tagaaacgat agcaaagtag ctgaagtaaa gaataaacca
     2521 actgatcata accctattca acaagcgcat ccttcaacgg ttacatccga tcattcagca
     2581 aagaaagaaa agccaatgct aaaagggttc aatgcaccaa gtaacaaaga cttactttaa
     2641 aaataaaaag gcgaggttac aatacctagc ctttttatta tgtacaaata caaacataaa
     2701 catatacgta tacatgtata taaacatgta tacgtatata tccataaaca cgtatacgaa
     2761 atttagttaa gctataccta accgcttatt ccaataaaaa aagtgatcag ctttaaactg
     2821 atcactcacc aaaaacttca tcatacaagt tatctatcat ttcaccaagt aaagctgaat
     2881 ctgatcgaat tcctaacttt tcgctgattt tagatagttt ctttctttgt gatggttgaa
     2941 gcgtgaattg ataatttttc ttcttttcag aactatcact ttttagttta gctaccaaag
     3001 aacttgttgt ttctttaggt gtttgtttag gttcttcaac tgcttcaacc acttcaggaa
     3061 cttcttctac ttcagcttca gcgaattttt taaatctatt ttctttcact tcaatcactc
     3121 cttattcttg tgccgtatca attattttgt tgtatagcgt aaacgtgctt tcaaagaaat
     3181 ccttattctt acgatagata ttcttatcct tagcgaactc agcaatattc ttaccttcta
     3241 agctagttgc attaagcaat tccttctctt gtacatatcc aagataatta gataacgttg
     3301 gtaccaattc taggaactca cgacttgaat tagtattatg tttgatcctg ctaccgatcg
     3361 tagcgtattt agtttccatc agtgtttcac cagtcttaat atcaatcaac tgttttgcta
     3421 gtgcatcaat atctttacca agcttaagca aagcttcaaa accatcttta gatggatcag
     3481 aaatgcctac gacaagatca gatacagccc aagcattttg agctagtaga cctgcatcat
     3541 tatgagtatc aatcagaatt agatcatact tctctttaag ctgttcatag ttaacaacga
     3601 tccatctaaa cagtagggtt ctgttgttag gcatatcacg catttgcatc tcaacttgtt
     3661 ttaaatcttc acctgctact aaaatatcca tgttctcatt tatagctaaa ggttcagctg
     3721 atccatgaag atataaatga ttgattgtat tctctctttt cgctttactg aaagcatcag
     3781 gaaatcgttt tgataggttt tgcgacttat cttcatcaat atacagcacc ttcataccta
     3841 aattttcgac agcatatcca cctagattgt agattgttgt tgttttacct gttccacctt
     3901 tttcaatgtt tccagtaatg attgacaact tcttttccat gattaaacct cccttattaa
     3961 tcttttgtaa ctccattata cactataaac a
//
LOCUS       DAXWLG010000194         3981 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXWLG010000194 DAXWLG010000000
VERSION     DAXWLG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.199"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(147..1019)
                     /locus_tag="ACGE4T_002508"
     CDS             complement(147..1019)
                     /locus_tag="ACGE4T_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8387719.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(1104..2393)
                     /locus_tag="ACGE4T_002509"
     CDS             complement(1104..2393)
                     /locus_tag="ACGE4T_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8387720.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(2537..3049)
                     /locus_tag="ACGE4T_002510"
     CDS             complement(2537..3049)
                     /locus_tag="ACGE4T_002510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387721.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(3116..3259)
                     /locus_tag="ACGE4T_002511"
     CDS             complement(3116..3259)
                     /locus_tag="ACGE4T_002511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8387722.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(3742..>3981)
                     /locus_tag="ACGE4T_002512"
     CDS             complement(3742..>3981)
                     /locus_tag="ACGE4T_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8387723.1"
                     /translation="MRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSK
                     IRNEVDKKLFQARVTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         1189 a          916 c          596 g         1280 t
ORIGIN      
        1 tttccaaggt aaacggctgt ggtatcaaac tagtaatcgt ctgataggca tctttcaatg
       61 gcttaaaaga attggccata ataaacaata atggatagat aataatgatt atgatgaaca
      121 taaataagag atgtggcaac cagcgtttaa ttttcatagt aaaccccctt ttccacaaat
      181 ttaaatatta cccctaagaa aacagcaaat attaataaag ttaaaactga aattgctgaa
      241 gattctccga ctttatacat ttcaaaagcc ttttgataag cataatagat cacgtttgca
      301 ctaccattat tcggtccacc acgagtgatg atcttaatcg gagtaaacac atactgcaga
      361 ccttgaacaa tggtcatcac caataaatat agtcctgtcg aactactcat aggcaacaca
      421 attgtaaaaa aaatgcgcca gatgggtaca cgatccaact tagctgcttc gatcatctct
      481 tgatcaattc ccacaattgc tgcaaaaatg acaatgaaat tataaccaaa aactttccaa
      541 gcagtaatac ctgcaatcac agcaattacc caaccttgta aattcatcca cataggcagt
      601 cttaaaccaa aatttcccaa caagacagca attggtcccg ataccggatt taatatccat
      661 gtaaaaacca tccccgccac taccaaagaa atcacagctg gaataaaaaa taatggttta
      721 tacaaattct gccctttttc tataactatt cctaataaaa aggatgtaat atatgggaac
      781 acacagctca gcaagacaaa tagaacggta taggacaaag tattcttcaa cactttatga
      841 aagactggat cagtcagcac ctttacatag ttggcaaagc caacaaattc tttagtgggg
      901 gccaccatat tccagtcaaa aaaactcaaa tagatcgtat aaatcaatgg ataaaaaaca
      961 aacacgccca acaaaataat tgctggcgcc agatacaaat aacctacata gcgcttcatt
     1021 tccgacagcc tcctcatcct tatgtcaaaa gcagacgacc ttgtttttcc tttcttacag
     1081 gcgctgtcgt ctgcttgctt tgcttatttg agtaactcat taatttcctt ttgagtagct
     1141 ttcatcgttt cattgacgga attacctgat aaaattttat cccgcatatc caacaatttt
     1201 tgttcagctt ctaatccagc atctccagga aatgaactcc aactgaccat cttatccatc
     1261 tgatcaattg ctggtatcat catctgattt tcagctaaga aagcttttaa tccattttgg
     1321 gcgtctgcaa catctttacg tggtggtaaa taaccagtac cttctgtcca tttagctaaa
     1381 gattcaacac tataaagata ttttaagaaa tcccaagttg cttccttttt cttatccgtt
     1441 tgtgatgtaa cagctaacat tgcgccacca gcaggtagtt taactttttt cccttcccaa
     1501 cttggtgact caactgcttt agcagtaaaa gtactatttt tttgaatgtt gtccctttga
     1561 gcaatagtgg tatagaccat cgcgacgtta ccatcaataa agctttgcac accttgttcc
     1621 cacgtagtgt gtaatgccgt ctggtctttt accatccttt gatataattc ataagcagcg
     1681 ataccatctt tagaagcaaa ggtagctttg ccatctttta ttacacttgc cccattactt
     1741 tctaaaattg cttgttgtgc ccaattatca gcaggttctt gaatatacac gccatacttt
     1801 ccagtttttt cttttattat cttagaaaaa gtttgaactt cttcccaagt ttttggacca
     1861 ttttcatcca agcccgctgc ttttaatata tcttgattta aatataaaac cggattacta
     1921 atggagtaag gaatacccac ttgtttacca tctttttcag acagtgccat gatattgtct
     1981 aaaaaatgat ctttaaaata atcagcatta tccggatcca cttcttctac taaagattga
     2041 ggattagtat atttgaaatt ttctgcaaaa taatctttaa aagcccatcc cacttgtact
     2101 acgtccggta tttctttgga ggcaacagaa gcttgtaaat tttgcatcaa gcctttatac
     2161 atatcaggat tatattttgc tacaaccttc actttgtctt gagattgatt gtatttatct
     2221 actaattcat tcactgtttt tccacctagg gtatccgcat taacatgcca atactctatc
     2281 gttacacgac cctccgattt ttttgttgtc ttggtattcc cactaccaca agctgccaaa
     2341 gagatgacac ttaaaacaga cacaacccct ataacaagat ttttctttaa cataacatta
     2401 acttcctcct aatttcttta tggccatttt tctaatcaag aaatattatg aactaccaat
     2461 gtaaatttct agttaatttt ctgtaaataa ttcgaaattt gtgcatttcg gggtacaggg
     2521 tcactggaca tatccatcaa gctaagaata aagcccccat atggtcatgt atcccatctc
     2581 caaatctctt aactcctctg ttttgattat aaaatagttt attctgtgct cctttcttcc
     2641 ataaataggc ccatgcacaa cgtgaggtta tattataaac accacagcgc gcaaggtact
     2701 caatcgtttc tagttcattg actgtccata cagaaaatgt caatcctagc tgctgcccca
     2761 cttcacgggc caacttagtt aaccatcctt catgaatatt gacgatttga acgctttgat
     2821 aggctaattt ctgtaaggta tcgatcaaat ctgcttcggt aatatcccca agttttttcc
     2881 cttcgaactg attttcaata ttataaaata tatgctttcc ccaaatcgca gcccaagata
     2941 attgtacata gccactaaga acaatttgtg gccagatttg ataggtttgt gccagcgaaa
     3001 aaacggccat ttccaatccc tcttctttta aatcacagtt aatggtcatg gttgccttct
     3061 cattggccac tgcttgaaac acctgctcaa gggttaccga atctgggaca acttcttacc
     3121 tgtcatgggc tagtattagc gtaccatctg tttgctgacg cacatctact tcaaaacaaa
     3181 gctggcgatc caaacaaaag cgaagaaatg cccagctatt ctcctccgta ttgtcactac
     3241 cagagtgtgc agtgatcatg gtcattttga cccctccttt aattccgtga ttttctccca
     3301 cacgtccact ataccgatga agcgttaatt tcttgttaat ttcttgtaaa ttccctgcgt
     3361 tttttcagta aactagttgt aaatcttgca tgctccttcc tacgaactca tgaatcgtcg
     3421 attttgccct tctagctagt ttcaccaatt aagtagttct acattccccg cttttaacct
     3481 actacacctt tttttgtttt cagctattcc ttcttcttgc tgaaagtcct tgaggttctt
     3541 tcttacagga taccatcaag gtgttcaatg gcttattccc attgaatgac cgagacttac
     3601 tatcagattt ctgctcgttg aaccaagtct ttgatacagg atttcttccg tcatcttctc
     3661 acaaatacca ccttgcgttt ctctagcgat tggtcgatgt gcaccctccc agtggactgt
     3721 cactacccct ttttgctatg cctagcccac aaaaaaagct gtcctcacag acagccagac
     3781 tgtagacaaa agctgttcca aactcgtaac tcgagcttgg aacagctttt tatctacttc
     3841 gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct ttccatagcc aggtggctaa
     3901 tttctttaaa ttcattgcag caaacactag catcgtatgc gtcgcgactt tttccaagcc
     3961 tcgatacttg gtccaacgca t
//
LOCUS       DAXWLG010000195         3907 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXWLG010000195 DAXWLG010000000
VERSION     DAXWLG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.200"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            72..758
                     /locus_tag="ACGE4T_002513"
     CDS             72..758
                     /locus_tag="ACGE4T_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFF8387724.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            760..1704
                     /locus_tag="ACGE4T_002514"
     CDS             760..1704
                     /locus_tag="ACGE4T_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFF8387725.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1903..2547
                     /gene="pcp"
                     /locus_tag="ACGE4T_002515"
     CDS             1903..2547
                     /gene="pcp"
                     /locus_tag="ACGE4T_002515"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFF8387726.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2626..3903
                     /locus_tag="ACGE4T_002516"
     CDS             2626..3903
                     /locus_tag="ACGE4T_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387727.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1250 a          677 c          861 g         1119 t
ORIGIN      
        1 aaaacaacta tattttttat gtgttttgct ttataattaa aaaaatattg tcattttatg
       61 gaggaaaata tatggaaaat tatcttagcc ttttaggcgt cttgattgtt attctcgggt
      121 ttgcatttaa attagactcg atattgatcg taatggttgc tttagttgtt acagctttga
      181 ctggcggtct tggcattcaa ggaatgttag aacttttagg tacaagtttt gtaaacaatc
      241 gcgggatggc gatttttatc atcattatgt tggcaacagg aacgcttgaa agaaatggtc
      301 tgaaagaatc agcggcttcc ttgatcaagc gattcaagaa agtatctgct ggtatgattg
      361 tagatattta tgggatcttt cgaatgatct ttgcggcttt taacgtgagt tttggtggag
      421 ttgctggatt tgttcgtcca atcattttac cgatggcatt ggggaccatc gaatcaaaag
      481 ggttagaagt ccatcctaag catgaagaag aactaaaagg tatggcttca gctatggaaa
      541 atgtctgctg gttctttggc caagtactat tcatcggagg agcaggggcg ttgctagtcc
      601 aatcaacact gaaagatcta ggatatgacg taacattggg acagcttgct ttagtggaag
      661 ttccagtggc tatcacggct ttagttgtgg caagtgtcta cttctatctt aaagaaaaga
      721 aattagcgaa gaaatattat ccaggaaagg ggcaataaga tggacttttt tacaaatcct
      781 gatattttat taggcgataa attactggag atcctttata tcgtgatggg actcgtattg
      841 atttacactg gagtaagaaa tttaacagat aaaaccaatc ctcatcgtta tgggtcagcc
      901 tttttctgga cggtcttagg ggtagttatt gcaggaggaa gatggcttcc ggcaatcgtg
      961 aacggtggct tgatctttgc gatgaccata ccagcaattg ctaagaaagt cagcaaagga
     1021 gaaagccggc ttccatcaaa agaatatatg gaaaaaatgt cagacaaact aggaatgaaa
     1081 atttttattc cagctttgag tatcggtgtc tttgcgatct tgtttgctct atttaccaat
     1141 ttaggtgctt tagtcggcgt gggcgtcggt gtttttgcag ccatgctaat cttgatgttc
     1201 ttttctcgag ataatcgacc aagtacattt ttagatgatg cagcagatat gctgggcaca
     1261 gtagggccgt taagcatgtt gccaatgctt ttagcttctc taggtgcggt atttacaagc
     1321 gctggagttg ggacagtgat ttccaatgca gtaggtacga tcgttccaga aggaaatgtg
     1381 aacatcggca tcatcattta cgctcttggc atggtgatat ttacggtaat catggggaat
     1441 gcatttgcgg ccatcaccgt gatgacggtt gggatcggtg caccattcgt atttagtcat
     1501 ggagcgaatc cagcgttaat aggaatggtt gcattgactt gcggtttttg tgggaccctg
     1561 ctgacaccga tggctgctaa cttcaatatc gtgcctgtag cgatgctgga gatgaaagac
     1621 aaatacggag tgatcaaaaa ccagttgttt attgcgttat ttatgctagt tttccaaatt
     1681 gcttatatga ttctatttaa ataatagaag agaggggttg tgacagaata attgtcgcaa
     1741 cctcttctaa cattgtagtg gaacactatt tttttataag aatatattaa gtttgatctg
     1801 ttatgttatt taaactattg tttcctttat ttacgctgtt caaggaaaat ggtatgataa
     1861 aagataacca tatgttaaat caatcggacg gaggaacaac agatgaaagt actagtaaca
     1921 ggttttgatc catttggtgg ggacaaagtg aatccagcct atgaagcagt aaaaaaaatg
     1981 ccagatgaaa tcagcggtgc tgaaatcatc aaagtggaag taccgaccgt attcgaaaaa
     2041 agcagtcaag tagtcaaaga agccatccaa cagcaccaac cagatgtcgt gatctgtgtc
     2101 ggacaagcag gtgggcggtc agcagtctcg ttcgaacgtg tcgcaatcaa tctagcagaa
     2161 gcaagaatcc ctgataatga aggaaatcag ccattcgata cggctttaga agaaaacggt
     2221 ccagccgctt actttacctc attgccaatc aaagcaatga caaaaaatgt acaagatcat
     2281 ggtctaccag cgtacatctc ttatacagca ggaacatttg tctgcaatga tatcatgtat
     2341 cgtttgcttc atttgatcga aacacaattc ccggcaattc gaggtgggtt catccacgtg
     2401 ccattctcac ccgatcaagt gatcgatcgt ccagtgggta cgccttctat gtctttagaa
     2461 gacattgctt cttctttgac ttatgcagtt gaagcagcag tagaaaataa agaagatatt
     2521 tccggaaatg ccggaacaac tcactaattt agcaagaaac actggctggt tgctaacgtt
     2581 atgttagtag ccagcttttt gtaattagaa aggaaagaaa cagccgtgga agaatggcaa
     2641 tccgtctttg aagaatggtt tccaaaagaa atcagcaaaa gctatcccat caaaatttct
     2701 aaacaataca caagcagtca acgttgggag atctatgcaa aattaacgaa aaaacagcgg
     2761 gaactagtcg ataagcaccg ccgatatcta atcagctctc gttttatgga agaacattat
     2821 ttagctgcaa cagattgggt gttttctgac ttcaagatca atccgttttt tcgaacaaaa
     2881 cgaagccagc aaaaattgta ttgtgagtgt ggtagagaat taaaagtgca atacatcgtt
     2941 aaatcgccaa aaaccggaaa gatcctaaaa ctaggcatca accactttgc cgatcacctg
     3001 catgtctctc cgacagtggc agcttcgatc catcagggaa tgacaaaagt tgatctggcc
     3061 ttagacgaat tattatggct taagcagaag aatatcgatt ttcctgaagg actatggcaa
     3121 aaatactgtt ttgtcttgta ccaaaatcgt cggatgaaac agccatattt accggatata
     3181 aaattagctc agcggctcgc agaatttcgc caagtagaaa tgccgattta catagcggat
     3241 tatcaagcat tggaaaatga aatcaagaaa ataagtgaac acatcaatgg gcagtcaaaa
     3301 aagcgacaga taaaaaaaga actatttgac gactttgcag aagaattagt aaaggatgta
     3361 gaagaattct tgatcaacta tcgcgcattt ttacgtaaag attggcaatc catcgtatat
     3421 gaagaagtgc cagtacatcc taatgcttat tttgaaacat ttatttccgt gttgagaaag
     3481 acaaaacgtc aacgaacacc agaagtcact gctcagatgg aatattttgc gaaaaaccaa
     3541 cgattcatcc aaccaaaaat ctatctattt atttggaagc aatattgtcg ctatggtttt
     3601 acagaagggt tcttcgacag cattcctcgg atagtcagaa atggtttttt aaaagtattg
     3661 cgtaaagaac gagaagctat acaatctgct gataagaaag accgtacagt ctcaaaagaa
     3721 aaatggcaac tagtagtgaa agatatccaa tctggaaatg tgcaagagac aatcgacaaa
     3781 tggaaaggaa aacattatcg ttttactgaa gctcagaaac aagcgttgga atattatcag
     3841 aagttagaag aatcactgcg tttcaatgat gaggcaagaa agtatctgaa ggaattacta
     3901 taaaaaa
//
LOCUS       DAXWLG010000196         3896 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXWLG010000196 DAXWLG010000000
VERSION     DAXWLG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.201"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..1377
                     /locus_tag="ACGE4T_002517"
     CDS             <1..1377
                     /locus_tag="ACGE4T_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8387728.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            1778..3358
                     /locus_tag="ACGE4T_002518"
     CDS             1778..3358
                     /locus_tag="ACGE4T_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFF8387729.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            3556..3780
                     /locus_tag="ACGE4T_002519"
     CDS             3556..3780
                     /locus_tag="ACGE4T_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFF8387730.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         1350 a          621 c          832 g         1093 t
ORIGIN      
        1 atgaatgctg accctgagag tcagtcgctg ttagcgcaga ttttattatt gatcgttttg
       61 acattgatca atgctttttt agcagcttcc gaaattgctg ttgtttcgat caataagaat
      121 cgaatcgagc aaaaagctga tgaaggagat gtgaaatctc aaaagctttt aaaaatattg
      181 cagaatccaa ataatttcct atcgacaatc caagtaggaa tcactttggt caacattttg
      241 tctggtgctt cactagctaa tactttatcg gaacggttag ctccggtatt aggtggcggc
      301 acagcggcaa aaaatattgc aaatatcatt gtgctggcta ttttgaccta tgtatcgatt
      361 gtttttggag aattgtaccc taaacgtatt gctttaaata aatcagaaga agtcgcaaag
      421 ttcacttcag ggctgatacg cttgattggt gtagtagcta agcccttcgt ctggctgctt
      481 tctgcttcca caagtttatt ggcgcgtatc acgccgatga cttttgatga tgaagattca
      541 aaaatgacca gagatgaaat gcgttatatg cttgaaaacg aaggcgtatt gaataatgaa
      601 gaattagaaa tgctccaagg ggttttttct cttgatacga aagttgcccg agaagtcatg
      661 gtcccacgaa cggatgcatt tatgattgat atcaacgaca ctgtggaaga aaatgtgaat
      721 gaagttttat ccgaaaacta ttcaagaatc cctgtttata acgaagacaa agataaagta
      781 gtcggaatcc ttcatacaaa gaatttacta aaagctgccc ataaattcgg ttttgataat
      841 ttagatatca agaaaatcat gcaggagcct ttattcgttc ctgaaacggt ctttattgat
      901 gatctgttat atgaaatgaa aaagacgcag aatcagatgg ctattttatt agatgaatat
      961 ggcggcgtcg ttgggttagc aacactagaa gaccttttag aagaaatcgt cggagaaatc
     1021 gatgatgaat cagatgaagt tgagaatctt tatgaaaaaa tcgatgaaca cgaatatatc
     1081 atccaaggac gtatgctgat tgatgagttc aatgaagctt ttgacagtga tctgcatatg
     1141 agtgatgtag atacgatggc tggttatttg attacagcat taggcatgat tccggatgaa
     1201 ggcgaaaagt tatcgtttga tgtcgataat attacgctgg tttctgaaga aatggaaggt
     1261 tctcgaatcc ttaaaatccg tgtcattttt catgatccgg aagaaacaga agcggaacct
     1321 gaagaagaac gaagatactt cagaaaagaa ttcgaagatg acgagccaag aagataattg
     1381 gaaacttggt tatgaaagac gtgttgcttg aataaacaag caacacgtct tttcttttag
     1441 tcagctgaca aaaaaatatt tgactcttat ggtttggtgg ctttttcatt cgatgaatcc
     1501 atgctatact gattcagttg tagtagttga caaaggttgt gaaaaagcta tcctgcatta
     1561 agtaataaga gcagccaagc aaaaacagct tttcgtattt ttggcttggg tgggcttatt
     1621 gctgataggc tgtctctaga acgccgttta ctcggaaaaa gagactgcta cacggctttt
     1681 gtcacagccc tatatgaaat tgtctttctt taagagatcg gacaatctgt gcctagagaa
     1741 aatataaata attgaatgga agaaatggag ttaggttatg aataatccag aattaaagca
     1801 acaagtggac aatcgcagaa cttttgcgat catttcccat ccggatgctg ggaaaacaac
     1861 gattacagaa caattactat tgtttggtgg ggctatccgt caagccggga ccgtaaaagg
     1921 caaaaaaaca ggtaattttg ccaaatcaga ctggatggaa atcgagaaac aacgtgggat
     1981 ttctgttact agttctgtta tgcagtttga ttatcaaggg aaacgagtaa atatcttgga
     2041 tacaccagga catgaagatt tttctgaaga tacttatcgt actttgatgg ccgtggatag
     2101 tgcggtcatg gtaatcgata gtgcaaaagg gatcgaagca cagacaaaga aattattcca
     2161 agttgtaaaa aaacgtggca tcccgatatt tacttttatc aacaaactag atcgtgatgg
     2221 tcgtgaacca ttggaattgt tagaagaatt agaagagcta ttagatatcg aatcttatcc
     2281 gatgaattgg ccaatcggta tgggtaaagg gctagaaggt ctttatgata tctaccataa
     2341 tcgtgtagag ttttatcgtc cggaaaatta tcaggatgaa cgattagtag aattggatga
     2401 agatggactg cttcccgaaa atcatcctct aactgaaaat tccttatatg aacaagtctt
     2461 agaagaagta gaactaatca aagaggctgg agatgcattt aatcaagaaa aaattgctcg
     2521 tggcgatcag acaccagtat tttttggttc tgcattgact aacttcggtg tacagacttt
     2581 tttagagact ttcgttgaat tggcaccagc tccttatgga cacaaaacac aagatgatca
     2641 gatggtaagt ccttatgagg aagaattttc aggtttcgta ttcaagatcc aagcaaatat
     2701 gaatccagcc catcgtgacc gaatcgcttt tgtccgaatc tgttctggaa cattcgaacg
     2761 aggaatggat gtctggttgg aacgtacaaa taaaaaactg aaattaagca atgtcacaca
     2821 atttatggca gattcgagag aaaacgtcga aaaagcagta gcaggcgata tcatcggtgt
     2881 atatgataca ggcaattatc agattgggga tacattgttc gaaggaaaac taaaagttgc
     2941 ttatgaagaa ctcccttcat ttactccaga gctgtttatg aaagtgactg caaaaaatgt
     3001 catgaaacaa aaatccttcc acaaaggaat ctatcaatta gttcaagaag gagcgattca
     3061 gctttataaa acatatctaa cggaagaata tatcattgga gctgttggtc agctacaatt
     3121 cgaagtattc caatatcgta tgttaaatga gtataacgcc gaagtaatca tgtctccaat
     3181 gggacacaaa atcgctcgct ggatcgatcc agaagatttg gatgagaaga tgagttcaag
     3241 tcgcaatatc ttggctcgtg accgtttcga ccaaccattg ttcttattcg agaaccaatt
     3301 tgcagaacgc tggtttgcgg ataaatatcc taatgtaaaa ttaaaaagtt tgatgtaaag
     3361 aagtcttgac acaagctgcc ctgcatcaag taataagaat agccaaacaa aaacagtctc
     3421 tcatattttg tttagagccg tgacggattt tccgtgacgg ctctttttac tatattaaaa
     3481 tcatagaaat gtctaatcaa aaaaatataa aaattatttt gcgtacatat gattaaagaa
     3541 aggagagtgt gacacatgga aacagaatgg caaatacgtg aaagaaaaaa acagaaaaaa
     3601 gaagagatgt tacgattatg tcgattatta gatttaaaag actgggacaa aaacgagcaa
     3661 attgcacaaa aagtacgatc aatcatccat aaagaggaaa aagttaaaaa taaagacacg
     3721 cgtagaactg aaaatgaaaa gagaaaagta caaaagaaaa gagaaaaatc aaaaagctag
     3781 aaagaaaaag aggctgagac agaagcgttt aactccaaga aataagctgg aattcacaaa
     3841 aatttgaaaa gcaattttcg tgaattttag cttatttccg aaggagttgc ttctgc
//
LOCUS       DAXWLG010000197         3874 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXWLG010000197 DAXWLG010000000
VERSION     DAXWLG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.202"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..355)
                     /locus_tag="ACGE4T_002520"
     CDS             complement(<1..355)
                     /locus_tag="ACGE4T_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387731.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(615..1163)
                     /locus_tag="ACGE4T_002521"
     CDS             complement(615..1163)
                     /locus_tag="ACGE4T_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387732.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1164..2021)
                     /locus_tag="ACGE4T_002522"
     CDS             complement(1164..2021)
                     /locus_tag="ACGE4T_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8387733.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2307..2593)
                     /locus_tag="ACGE4T_002523"
                     /pseudo
     CDS             complement(2307..2593)
                     /locus_tag="ACGE4T_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2583..2912)
                     /locus_tag="ACGE4T_002524"
     CDS             complement(2583..2912)
                     /locus_tag="ACGE4T_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8387734.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3261)
                     /locus_tag="ACGE4T_002525"
     CDS             complement(2989..3261)
                     /locus_tag="ACGE4T_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8387735.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3261..3524)
                     /locus_tag="ACGE4T_002526"
     CDS             complement(3261..3524)
                     /locus_tag="ACGE4T_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8387736.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            3758..>3874
                     /locus_tag="ACGE4T_002527"
     CDS             3758..>3874
                     /locus_tag="ACGE4T_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8387737.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNR"
BASE COUNT         1156 a          781 c          494 g         1443 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct aattggctca
      241 taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa
      301 acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt ttcatctaca
      361 tctcctccta atagttttat tttagagtta gctctattac ttatttacta actcaacaac
      421 agattatcat acacttgtta atgaaatcac aatatctcat taaaacacac cctaaaatca
      481 taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc
      541 ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag
      601 ttcttctttg attattatct aactttatta ttcattcccc accctttgcg cagtgagtct
      661 tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc atctaattct
      721 ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc attaaacaaa
      781 tcttgcttat cttcttgcgt aagattctgt ttaatatact catctaaagc ttttctaagc
      841 aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga agtcgcattt
      901 aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa ctttctgatt
      961 ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga ggaagttttt
     1021 atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat tgcttgctgc
     1081 tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt cccaaaaata
     1141 tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc gtgttcttcc
     1201 aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg ttttaagaga
     1261 cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa gcgcgcattt
     1321 ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg tttatacatt
     1381 tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc aacatataaa
     1441 tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat cgcatttctc
     1501 atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta catcgcggat
     1561 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
     1621 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
     1681 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
     1741 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
     1801 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
     1861 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
     1921 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
     1981 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
     2041 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
     2101 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
     2161 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
     2221 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
     2281 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
     2341 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
     2401 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
     3061 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
     3121 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     3181 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     3241 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     3301 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     3361 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     3421 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     3481 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     3541 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     3601 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     3661 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3721 gagtagtctc aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga
     3781 acattataca gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata
     3841 ccacaattgt gtaccagata agattaaaaa ccga
//
LOCUS       DAXWLG010000198         3833 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXWLG010000198 DAXWLG010000000
VERSION     DAXWLG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.203"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            180..653
                     /locus_tag="ACGE4T_002528"
     CDS             180..653
                     /locus_tag="ACGE4T_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFF8387738.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            650..2782
                     /gene="feoB"
                     /locus_tag="ACGE4T_002529"
     CDS             650..2782
                     /gene="feoB"
                     /locus_tag="ACGE4T_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFF8387739.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2784..2918
                     /locus_tag="ACGE4T_002530"
     CDS             2784..2918
                     /locus_tag="ACGE4T_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFF8387740.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3012..3656
                     /locus_tag="ACGE4T_002531"
     CDS             3012..3656
                     /locus_tag="ACGE4T_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8387741.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
BASE COUNT         1197 a          686 c          843 g         1107 t
ORIGIN      
        1 atttttatct atcaatacta aagattcgct cataatatca caagtataat tgacattctc
       61 aattaccgag agtattctat gtgagaatga ttctcattta tttgcgtaac acgtaagaaa
      121 attagaacat ataatgaatg attttaatac atgatttatt tcacaagaga ggaatcaata
      181 tgcagacctt agcgcaggca aagcagaatt tcgtttatca agtcgaacga atcgaaacag
      241 aagaaaaaac aaaacgccat ttgcaaaata tcgggatcgt tgccggaagt aaaatcgtcg
      301 tcgtcaattt atcaggcaac aatgccattt tactggtaaa aagtcatcga gtggcagtaa
      361 gtcgggaaat actaggtcaa atctttgtca ataaaaagac tgcttcctct gaaagctgga
      421 cttctcttga tcaattaaaa gcaggagaga ctgggatcgt cgtagcaatc cacggacaag
      481 gcgcagtgaa gcgacggtta atggatatgg gattgacgcg aaatgtaaaa atagttatcc
      541 gaaaactagc ccctctggga gatccaatcg aactgacagt gcgagggtat gaactgacgt
      601 tacgaaagtc agaggccgaa ctaatcttga tacaaaggga ggaaaaagaa tgaatatcgc
      661 gttagtcgga aatccaaaca gcgggaaaac tagcttgttc aatctcttga ctggttcgaa
      721 tcaatatgta gggaattggc cgggcgtaac agtagaaaaa aaagaaggat cgttgaaaaa
      781 aaatccgaac attcatattc aggacctacc cggcatctat tctttgtctc cttatacacc
      841 agaagaagtg attgcaagag attatttggt tcaggaacag cctgaactga tcatcaacat
      901 tattgatgct accaatttag aacgaaatct ttatttaact acgcagttat tggatttaga
      961 cattcctatg atcattggtc tgaatatgat ggatgtcatc aaaaaagaag gaaaaaaaat
     1021 caatctagaa aagctttcct atggtcttgg tgtaccagtt ttaccaatca gcgtattgaa
     1081 aaagcaagga atcgaaaaac taatcgaaaa gattcaggag tctgcaaaaa agcggccttc
     1141 tattcctgtt ccctgtacat atgatccacg tttagaagca gctttacatg aaattgaaga
     1201 tgtactagga gaacaaagcg gcaagaaacg ctggtatgca atgaaatatt ttgaacgaga
     1261 tcagaaggta agagaggatt acgagatagc agtttctcag caaaaagaga tcgagcaact
     1321 gatccagtta acagagaaac tattggacga cgatagtgag acgattctgg tcaatgaacg
     1381 ttatgaattt attacccaac tatgtacact ctgtgttgtg tcaaatgatt ctttccaatt
     1441 atcgatgagt gacaaaatcg atcagattgc aacgaatcgt tggttagcct tgccgatctt
     1501 cgcatttgtg atgtggctga tctattatct ggctattcaa acagtaggaa caatggggac
     1561 agattggatc aatgacacat tgtttggtac atggttacca gaacatgttg gcagattgtt
     1621 agctgaatgg caagtagcgg gatggatgca ggagctgatc ttgaacggca tcatcgcagg
     1681 cgtgggagca gtgttaggat ttctaccgca attgattata ttgtttttat gcttgagctt
     1741 tctggaagac tgcggatata tggcgaggat cgcttttgtc atggatcgac ttttcaggaa
     1801 attcggtctt tcaggaaaat ccttcattcc tatgctgatt gctacaggat gtggagtgcc
     1861 aggcgtcatg gccagtcgca cgattgaaaa cgagcaagat cgtcgtttga cgatcatggt
     1921 cacaaccttc atgccttgtt ctgcaaagtt gccgattatt gccttagtag caggggcctt
     1981 ttttcctcat caatcttggg tagctccttc tgcctatttt cttggtatga ctgcaattat
     2041 tttctctgga atcagcttga aaaaaacacg tttgtttgct ggagatacag ctccttttat
     2101 catggaattg cccgcatatc attttcctca atggtcagcg gtcctaagac aaacctatga
     2161 ccgcagcaag tcttttgtta aaaaagcagg aacgattatc tttgtttcta gtatcgtgat
     2221 ctggttcagc tcttcttata atttttatgc tcaacctgtt tctgaagacc aaagtatctt
     2281 agcctttttc gggcgaatcc tggcagttgt ttttcagccg cttggttggg gaaactggca
     2341 aggaacagtc gcagcgatta caggattggt cgctaaagaa aatattattg gcacatttgg
     2401 tattttgttt ggtcatgcgg aagtatccga aaatggcaga gaaatctggc aagtgctaca
     2461 acacacatat actcccgcag cagcttattc attgttagcc ttcaatttac tttgtgcgcc
     2521 ttgttttgcg gcaattgggg ctatccatcg ggaaatgaat gccaagaaat ggacttggat
     2581 cgccataggc tatcaatgcg gtttagctta tttagttagt tttgtgattt atcagttggg
     2641 acacttattg gagggtggac aggtagcgtt agggacgtat cttgctattt tactgatgat
     2701 aggactcgtt tatgttttgt tccgtcagcc aaaatccaag aaccagtttt atactatatt
     2761 gtcattagaa ggagaggaat aggatggcga cattcttatt aagtgtgtta atctttggaa
     2821 gtgcaggcgc aattgtctat actcgtttaa aatccggtaa gaattgtgat gactgtcaaa
     2881 cagcttgccc agtaaaaaag gaacagatca agcgatgatc agtcgttgca agaggctatt
     2941 ctcccctttt ttgcttattt tgctagagca tagtaagatg agggaaagta aaaaaagaaa
     3001 gaggaaagaa aatgtctgga aagaaaaaaa tcgtccgccc gcgctaccag cagattgctg
     3061 ttgatttagc cgaacgtatc gttgaaaacc gatataaagt gggtgagaag atccatgccc
     3121 gctcaacact tgccagtacg tacaatgctt ctcctgagac tacccgcaag gctatcaatg
     3181 tgctggtaga tcttggaatc atggaagtac ggcatggaag tggggcattt gttgcttcga
     3241 aggaaaaagc aaaagatttc ctattgcaat atcaagatgt acaatccatc caagatacaa
     3301 aagcagaaat catggcaagt gtggagaagc aacaagatga gctgaatcat ttttctcaat
     3361 tattggatca gctggtcaat caaacgaagc gaatacataa gatgaatcct ttacttccgt
     3421 ttgaattatc tttatcagaa aaagcaatgc atctggaccg ttcaattagc gagttgaata
     3481 tctggcaggc gactggcgcc acagttatcg caatttctca taatgaagaa ctgcttgttt
     3541 cacctggccc ctatgctaaa tttacggcag gggacatcgt tcattttatt ggaaatgaat
     3601 atacactaca gcgtatgaca aacttttttt acccggagca tcagcttgca gaatgattgt
     3661 gtgaaatatt aaaaaaatat taacttggct aaacctttta tttacaaaga tttagccaag
     3721 tttttttcag tttggacaag caaatttctt tcatttttga caaagtgact acatgatgtt
     3781 aacatagtgt ggtcactttt ttcagaaaga aaaatgaatt tacataaaaa agg
//
LOCUS       DAXWLG010000199         3776 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXWLG010000199 DAXWLG010000000
VERSION     DAXWLG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.204"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE4T_002532"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE4T_002532"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFF8387742.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACGE4T_002533"
     CDS             complement(1172..1867)
                     /locus_tag="ACGE4T_002533"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFF8387743.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACGE4T_002534"
     CDS             complement(1880..2596)
                     /locus_tag="ACGE4T_002534"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFF8387744.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="ACGE4T_002535"
     CDS             2926..3531
                     /locus_tag="ACGE4T_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFF8387745.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
BASE COUNT         1078 a          774 c          669 g         1255 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctc
//
LOCUS       DAXWLG010000200         3765 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXWLG010000200 DAXWLG010000000
VERSION     DAXWLG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.205"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(5..1771)
                     /locus_tag="ACGE4T_002536"
     CDS             complement(5..1771)
                     /locus_tag="ACGE4T_002536"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387746.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQGGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAACL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKVVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1731..2429)
                     /locus_tag="ACGE4T_002537"
                     /pseudo
     CDS             complement(1731..2429)
                     /locus_tag="ACGE4T_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2517..3596
                     /locus_tag="ACGE4T_002538"
     CDS             2517..3596
                     /locus_tag="ACGE4T_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8387747.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         1122 a          827 c          719 g         1097 t
ORIGIN      
        1 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
       61 ttatgagatt tttgtatatt tctgaccttc gatacaactc actatgagta ccttgatcaa
      121 ctacttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
      181 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
      241 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
      301 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
      361 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
      421 ctaaacaagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
      481 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
      541 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
      601 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
      661 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
      721 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
      781 tatctgatcc cccttggata ctggtttcct tcatagctct aattcgctgt attttaggag
      841 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
      901 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
      961 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
     1021 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
     1081 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
     1141 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
     1201 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
     1261 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
     1321 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
     1381 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
     1441 gtaaatttga caaatcgtct gcaatttgca ctcttaaatg cgcactttct ttataagtac
     1501 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacaa
     1561 acgatacggc aaaataaagc caacggggct ttgtattttc taaaactaat tcgtctatca
     1621 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaaa ctagaaatag
     1681 aagctttagt taaagcttct gcgccctgat cacttaaagc aaatttgttt ttataaaaac
     1741 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
     1801 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
     1861 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
     1921 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
     1981 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
     2041 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
     2101 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
     2161 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
     2221 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
     2281 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
     2341 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
     2401 cttttttgtt ggcggatttt ataattaact tagccatttg gaaataaaaa aacgccacgt
     2461 gaattccatg ttatattgaa gttaccacaa ctctcaatag acaaggatga attcacatga
     2521 cgcaaaacca tgataacaca gtttcacgca agggaaaaca cctaacctac tcagaacgtt
     2581 gccaaatcgc catcctgaaa aaggaaaact attccaaccg tcaggttgcg gatgtcctgg
     2641 gctgcgtgcc gcagaccata aacaacgaaa tcaaacgcgg gaccgttact cagcttaacc
     2701 gccagaatca gaacgggaag gtctacgact attatcatga cgtctacgac gccgatgccg
     2761 ggcaggcggc ctacgagaag aaccgcttga actgcggcag acggccctta tgggccgaca
     2821 cggacgcctt cattgaatgg gcggacgaca tgatgctgtt ggaaaaatgg tccccggacg
     2881 tggtggtggg ctttgccaga aaacacgaat tgttcgaccc ggccattatc cctgggacca
     2941 ccaccctcta tcagtggatc gaccgtggca tcatgaaaac gaagaatatg gacctgctag
     3001 aaaaactgtc ccgcaaaccg aaatcgtccc gtcatggcaa ccgcccgaat aaacgggtgc
     3061 tagggaaatc cattgaagag cgcccggagg cggttggtac ccggaaaacc ttcggccatt
     3121 gggaagtcga cacggtcgta ggaaacaaga ccaagtcgga tgccgtgctg ctgacgttgg
     3181 tggaacggca gacacgcttc gaggtcattc tgaagctggc cgggaaggat gccgaatcgg
     3241 tcaaccaagc ggtccgacag ctgagagaac gggctggtgc gtacttcggc cagctgttca
     3301 agacggttac ctcggacaac ggctcggagt tcgcgggact gcacgatgcc ttgcaggatg
     3361 tggcaaacat ctacttcagc cacccctatg cttcctggga gcgcgggacg agcgaaaacc
     3421 aacacaagct gattcgccgt ttcatcccca agggcagccc aatcggcaac gtgagcgaaa
     3481 accaaatctt gcggattcag cgttggatga acgattaccc gcgaaagatt ttgggctatg
     3541 caacgccgca tgacacgttc gtccaggcat ttaggcagga acggttgacg gcttgacacg
     3601 tgggcccttg acaacgagcc tccctcggca tggcttgtgt ctggaaagct ggccggggcc
     3661 agccagtcgg gagactacca tgcctcagct cattgtcaag ggcccactcg attagaaaaa
     3721 ttagacggtt ggtggtaacg agcaaccggg tcaccatggg tggct
//
LOCUS       DAXWLG010000201         3726 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXWLG010000201 DAXWLG010000000
VERSION     DAXWLG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.206"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(46..780)
                     /locus_tag="ACGE4T_002539"
     CDS             complement(46..780)
                     /locus_tag="ACGE4T_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8387748.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="ACGE4T_002540"
     CDS             1106..1516
                     /locus_tag="ACGE4T_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFF8387749.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="ACGE4T_002541"
     CDS             1509..2210
                     /locus_tag="ACGE4T_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFF8387750.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..>3726
                     /locus_tag="ACGE4T_002542"
     CDS             2210..>3726
                     /locus_tag="ACGE4T_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387751.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEEL"
BASE COUNT         1141 a          680 c          740 g         1165 t
ORIGIN      
        1 cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag ccataaataa
       61 taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa ctggtaccca
      121 tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt gtacaaaacg
      181 tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa gtgaagcaac
      241 taatgtccat acaaggaaac cgagtgctaa gtgagctgta ccaagattat cttggaagaa
      301 tggtgctttt gtaataccta aggcaccaaa gaataaaacg aatgaaccaa agaattcatt
      361 gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac ctaacacatg
      421 gttcacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga tcaattgtcc
      481 aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc aagggaatag
      541 accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc tgattccacc
      601 aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca gcatccagtc
      661 actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt tacctagaac
      721 gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg agtaatccat
      781 aaatacctcc aaaagttata aaaatgcaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaacca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagctatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agagggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc aggaagggta tctgtaaacg aaatggagga
     1501 aaatcaaatt gactgagttt tttgtgaatc cactatttgg cttaagtttg actttaggag
     1561 tgtatgtatt atctgataaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat cggtacagca aaagcaatcg
     2101 agattggcga aatagaagga atcatttccg gacttgcgat atgcgtaaat gggatattga
     2161 ccgttttgtt gctgccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatacatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaagtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg ggtcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgaccgaaga
     3721 actaaa
//
LOCUS       DAXWLG010000202         3687 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXWLG010000202 DAXWLG010000000
VERSION     DAXWLG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.208"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(147..320)
                     /locus_tag="ACGE4T_002543"
     CDS             complement(147..320)
                     /locus_tag="ACGE4T_002543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387752.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            606..1934
                     /locus_tag="ACGE4T_002544"
     CDS             606..1934
                     /locus_tag="ACGE4T_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFF8387753.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2136..2969
                     /locus_tag="ACGE4T_002545"
     CDS             2136..2969
                     /locus_tag="ACGE4T_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFF8387754.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2985..>3687
                     /locus_tag="ACGE4T_002546"
     CDS             2985..>3687
                     /locus_tag="ACGE4T_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFF8387755.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQL"
BASE COUNT         1224 a          585 c          768 g         1110 t
ORIGIN      
        1 ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt
       61 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
      121 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      181 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      241 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      301 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      361 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      421 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      481 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      541 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      601 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      661 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      721 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      781 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      841 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      901 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      961 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
     1021 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1081 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1141 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1201 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1261 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1321 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1381 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1441 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1501 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1561 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1621 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1681 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1741 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1801 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1861 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1921 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1981 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     2041 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2101 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2161 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2221 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2281 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2341 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2401 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2461 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2521 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2581 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2641 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2701 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2761 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2821 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2881 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2941 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     3001 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3061 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3121 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3181 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3241 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3301 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3361 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3421 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3481 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3541 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3601 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3661 atgaaatccg aatgcaagaa cagctgt
//
LOCUS       DAXWLG010000203         3674 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXWLG010000203 DAXWLG010000000
VERSION     DAXWLG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.209"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(95..1030)
                     /gene="murB"
                     /locus_tag="ACGE4T_002547"
     CDS             complement(95..1030)
                     /gene="murB"
                     /locus_tag="ACGE4T_002547"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFF8387756.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1103..2023)
                     /locus_tag="ACGE4T_002548"
     CDS             complement(1103..2023)
                     /locus_tag="ACGE4T_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8387757.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2136..2888
                     /locus_tag="ACGE4T_002549"
     CDS             2136..2888
                     /locus_tag="ACGE4T_002549"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFF8387758.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT         1068 a          733 c          643 g         1230 t
ORIGIN      
        1 tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc
       61 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
      121 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
      181 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
      241 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
      301 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
      361 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
      421 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
      481 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
      541 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
      601 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
      661 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
      721 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
      781 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
      841 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
      901 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
      961 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
     1021 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     1081 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     1141 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     1201 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     1261 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcatcttga atgacaagct
     1321 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     1381 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     1441 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     1501 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     1561 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     1621 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     1681 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     1741 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     1801 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     1861 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     1921 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     1981 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     2041 catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat agtatcgcaa
     2101 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     2161 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     2221 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     2281 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     2341 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     2401 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     2461 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     2521 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     2581 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     2641 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     2701 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     2761 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     2821 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     2881 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     2941 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     3001 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     3061 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa
     3121 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
     3181 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     3241 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     3301 aaacaaaaat agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct
     3361 tttgaacacc gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa
     3421 caaatggcaa aatagttcct catattttgc tattttttga gctaataccg agggtcagct
     3481 gattgaacac cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa
     3541 atttttttga aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca
     3601 tgatccccat ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa
     3661 atattacata aaaa
//
LOCUS       DAXWLG010000204         3618 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXWLG010000204 DAXWLG010000000
VERSION     DAXWLG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.210"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            21..245
                     /gene="nrdH"
                     /locus_tag="ACGE4T_002550"
     CDS             21..245
                     /gene="nrdH"
                     /locus_tag="ACGE4T_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFF8387759.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            404..2563
                     /gene="nrdE"
                     /locus_tag="ACGE4T_002551"
     CDS             404..2563
                     /gene="nrdE"
                     /locus_tag="ACGE4T_002551"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFF8387760.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2613..3578
                     /gene="nrdF"
                     /locus_tag="ACGE4T_002552"
     CDS             2613..3578
                     /gene="nrdF"
                     /locus_tag="ACGE4T_002552"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFF8387761.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
BASE COUNT         1236 a          663 c          682 g         1037 t
ORIGIN      
        1 tgttagagga ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat
       61 gtaagatggt caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg
      121 atgaacagcc tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca
      181 ttacttctga tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca
      241 gctaacatga ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac
      301 cagtttttgt cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg
      361 caatcaagca tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt
      421 acaagatgtc agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat
      481 ccctttgaat aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa
      541 tacgatgact tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat
      601 agaagcagaa ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt
      661 ggacgagcaa catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata
      721 tgcgttgaaa accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt
      781 caatgcgctt tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat
      841 gatccatcaa cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg
      901 gggcgaattg gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg
      961 ctcaatcaac tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc
     1021 aaatcttcgt gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt
     1081 tccagtcatg aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca
     1141 aggagctggc gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc
     1201 aaaaaaagaa aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt
     1261 acctgataaa ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata
     1321 tagtgtcgaa aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga
     1381 tcatctagtt gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa
     1441 tgaaatctca aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc
     1501 gaaccgcgag aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt
     1561 acaagttcaa acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga
     1621 tatctcatgt aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg
     1681 tcaatctgtg cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga
     1741 tgtcgtacca ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat
     1801 gggcttacac acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga
     1861 ctttacaaat atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat
     1921 cgctcgtgaa agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac
     1981 ttatttcgaa aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt
     2041 gtttgaaggc atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat
     2101 gaaagatgga ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata
     2161 tatcaacgat acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga
     2221 gaagaaaatc ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta
     2281 ttacacttct gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca
     2341 acaacacgtc gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg
     2401 catttatgaa tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca
     2461 ttacgctttc cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc
     2521 agaagaaatc ggaagcaatc aatgtgaaag ctgcgtgatc taataccata gatagattcg
     2581 cttattcata ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa
     2641 actggaatga aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat
     2701 tttggctaga tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc
     2761 aattggaaaa agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc
     2821 aatctgaaag tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg
     2881 ttttgaacaa tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta
     2941 gtacactgaa tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt
     3001 atctgcaaaa aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa
     3061 agaaaattgc cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc
     3121 tttattattt aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc
     3181 gagatgaatc tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt
     3241 tacctgaagc agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt
     3301 acgaaaacga agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag
     3361 tcaagacatt cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac
     3421 tctttccaga tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca
     3481 caagcaacca tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag
     3541 cgatgaaaga cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat
     3601 ttttcgaaaa ataaaaag
//
LOCUS       DAXWLG010000205         3581 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXWLG010000205 DAXWLG010000000
VERSION     DAXWLG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.212"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            77..1366
                     /locus_tag="ACGE4T_002553"
     CDS             77..1366
                     /locus_tag="ACGE4T_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8387762.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1492..2571)
                     /locus_tag="ACGE4T_002554"
     CDS             complement(1492..2571)
                     /locus_tag="ACGE4T_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8387763.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2680..3120)
                     /locus_tag="ACGE4T_002555"
     CDS             complement(2680..3120)
                     /locus_tag="ACGE4T_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8387764.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3177..>3581)
                     /locus_tag="ACGE4T_002556"
     CDS             complement(3177..>3581)
                     /locus_tag="ACGE4T_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8387765.1"
                     /translation="KMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPEQL
                     FAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLSLE
                     ETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         1340 a          688 c          633 g          920 t
ORIGIN      
        1 aaaaacagta aacatatagg tagaatctct actatcttat ttcttgcata caaaaaaatc
       61 aaggagtgag agagttatga gatattcaac catcttaata tcaacatttg cagccttaag
      121 cttatcgctt actataaatc agacagtcca cacagaggag atagaaacag ccaaatggac
      181 agaaaccaag tgggccaacg tcaatcgaat tacctttcag gatgaatttt catccttcaa
      241 ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa aaattgacta
      301 ttcaggagac ttagcaccta tactacaatc agctgttaca tttgagaatg ttgatcccgc
      361 tttgtcgatt ggctatgacg agaacacttt gactatccaa cacaagcaag cgatatttac
      421 cttcaccgtt acattaaatc aggacttgtt aaaaccagcg ctttttactg tcaaggtatc
      481 agatggacta ccgaacgatc atcatcattt aacgccgtat agtcagcggc agacaattga
      541 gcaagattca gctatcgact caggaggaga tcttgttgaa gaaccaacag ataaaccaga
      601 aaatgaaaat aagccagaag taccgcctac agaaaatcca gacggagaac agaaaccgga
      661 aatagaacct ggagaagaac ccgatacaga aactcaacca gagccagaca atgaatcaaa
      721 acctgaaata acccctggag aaaaaccaga tgtagaccca gaagaaaaac cggatgtgac
      781 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
      841 taatgaaact gaaaatccag aaaaaccaga ggtagcccca gaagaaaaac cagatgtaac
      901 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
      961 taatgaaact gaaaatccag aaaaactaga ggtagatcca gaagaaaaac cagatgtgac
     1021 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
     1081 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
     1141 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
     1201 aaaaagtagc aaggatacaa cctttgctag tcctgaaacg aaaaataaac agctaccaaa
     1261 atccggagag tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc
     1321 aacaatgctt tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt
     1381 ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa acgaccccaa aataccagat
     1441 tgaaatctga tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat
     1501 atctgtttct tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa
     1561 ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag
     1621 taagaccaca aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt
     1681 cagcacataa gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc
     1741 tcggatgcct ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc
     1801 tgctactggg atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa
     1861 gccaaataca gccaaggcga ttgtagtgag caacgtgttt aaaagagcga taacaaattg
     1921 agcttctaat accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg
     1981 aaagaaccaa gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt
     2041 ttcaatcata aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc
     2101 tatatcctgt aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc
     2161 gtttcgctca atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg
     2221 ctcgtaaaaa tcaataacgg actggatcat atcaaatgtt tgatgaataa gcatcgggat
     2281 atactttgtg aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa
     2341 aacacctgta gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata
     2401 agtaaagata aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa
     2461 aacgacaaat aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata
     2521 aacggtcaaa tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg
     2581 gaatatgaaa ataagaaaga gaaagagcaa aaagaaaaac cgctaaatga cgttatctta
     2641 tgagttggtt ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg
     2701 atgaaaatta tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc
     2761 aagtggaaga taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc
     2821 accttgagga gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt
     2881 aatataattt ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca
     2941 caaatcattt cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac
     3001 aagatactgg gtataggtaa gattcagcgg cttgaggagg gaagtgtagt gtctaatgat
     3061 ttccttagag gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat
     3121 ttttccacct ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat
     3181 tcactaactg ctttcagagt aacttctata tttccagctg tagcttttga atacggacac
     3241 actttatgcg ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat
     3301 ccttcaatct ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt
     3361 gcactgacag tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg
     3421 ttaaaacatg cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg
     3481 cgtttaccag gagctgcaat attcaattcg aatgtacgat cgggattatg aacttctcca
     3541 gagcgtccgc ctgtgttgat cattttcgta gaatacattt t
//
LOCUS       DAXWLG010000206         3575 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXWLG010000206 DAXWLG010000000
VERSION     DAXWLG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.213"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..278
                     /locus_tag="ACGE4T_002557"
     CDS             <1..278
                     /locus_tag="ACGE4T_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387766.1"
                     /translation="RAYYSAFKKSDEYLKENYDIIYNGSSGKGSHQTVWNLFSTVKEL
                     NTLGIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
     gene            complement(314..616)
                     /locus_tag="ACGE4T_002558"
     CDS             complement(314..616)
                     /locus_tag="ACGE4T_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8387767.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(874..987)
                     /locus_tag="ACGE4T_002559"
                     /pseudo
     CDS             complement(874..987)
                     /locus_tag="ACGE4T_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1370..2602)
                     /locus_tag="ACGE4T_002560"
     CDS             complement(1370..2602)
                     /locus_tag="ACGE4T_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFF8387768.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2856..3425
                     /locus_tag="ACGE4T_002561"
     CDS             2856..3425
                     /locus_tag="ACGE4T_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8387769.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
BASE COUNT         1166 a          637 c          651 g         1121 t
ORIGIN      
        1 caagagcata ctacagtgct tttaaaaaat ctgatgagta tttgaaagaa aattatgata
       61 ttatttataa tggaagtagc ggaaaaggaa gccaccaaac cgtttggaat ttgttttcaa
      121 cagttaagga attgaatacc ttaggaatac ataatagtgg gtatagaatg ttagagaaaa
      181 gaaaatgtgc tgactatatt cctaatgaaa ccattacgaa aacagatatg gcgctgatga
      241 atcgagaagc agaaaaaata ataaacaaac tcacctaatg aatttcaagc gagtttgttt
      301 gttatttttt tatttaatct ttgtatcttt ctggatcaac gaaagtatac tttatatagt
      361 cataacgccg atgatcgcta cgtgcgtccg gcacgtcagt cacgatatca aacaaaaagt
      421 atacaccctt cttcattcta gttttcgcag caggaatttt gaaatagttc ttattagaat
      481 agtagagatt gattaataag ctatcttcga ttgctaaaaa gaaaacttct gaattccata
      541 ctttataaaa atctttgata aatctattcg aagggtcaaa tttaaaccat aattgtgttt
      601 ttccttccat cagcataaaa gttcacctca aaaagagtat acgaacgaat gttctttttg
      661 tgaaggcgga atttaatata ctgaataata gaaaacaagt acttgtgcca aattatgtgc
      721 caaaaaaatt cgaattcaac aaaatgtaaa caaatagaaa accttctata ttaagatttc
      781 ctgcaataac aaacacaaca gaagacgtgt aatagttagt caggaacgta caaataaccc
      841 ctgtatcctt tgcggtacag ggggttattt tgtataagta gacattgtag tttctaaact
      901 gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga
      961 tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga gaaaataggg aaaaattgat
     1021 tactaaagaa aaacttacta ggtcgacggt gtagttgatt ttcatacttc aatagattcg
     1081 aattaaacga tttcgattgg cttataccag tcctatccaa cgaaagatat atcgttctta
     1141 actctagcgg tataacttga agctttacgt aaagccaatg ggaatgaact ggagatgact
     1201 ttttattatt tttagtaata gctgatgggt taatacttac aactttcctt ttcaaagtac
     1261 catataaaaa aacggtgggg ttgtttagcg aaataaagga tctaagccaa tagcttagat
     1321 cctttattct atgctttata ttattttttg catgtgtgcg aaagtatact caagcccaat
     1381 cgccatttcg gaaaatcggt actgtagagc catcttcacg gattccgtcg atatccatct
     1441 tgtcagatcc taccatgaaa tctacgtggg tttgacttct attcaatcca gcttctgcta
     1501 attcttcatc ggacatttct gtaccgcctt tcacactgaa ggcataagca gaacctagtg
     1561 ctaagtgatt cgatgcgttt tcatcaaata atgtattgaa aaagatgatg ccagattgag
     1621 aaataggtga agggtcaggt acaagtgcga cttctcctaa acgacgcgca ccttcatctg
     1681 tatcaagcag ttttgctaag acatcttctc cttgttcggc agaaaaatca acaacttttc
     1741 catctttaaa agtaaatttc atacccgaga tgatggtgcc agcataactc agtggctttg
     1801 tacttgaaat gtaaccatct actcgatgac tgtcaggtgc tgtgaacact tcttccgtcg
     1861 gcatatttgc catgaatttt tctccacgag cattatagct tccagcgcct tcccaaagat
     1921 ggtttttagg caatccgata atgatatctg taccgggagc agtatagtga agggcagaaa
     1981 attgttctcg attcaattct tctgcttttt ttgctaattt ttcatcatgc ttcttccatg
     2041 ctaagacagg gtcttcttca tagacacgag tcgttttgaa aatctgatcc cataatgcct
     2101 caacttgttt ttcttctggc agttctggga agacttttgc tgcccattgt ttaccagccg
     2161 cagccacgac agtccaactg actttatttg cttgagttgc cttgcgtaaa ttcattagtg
     2221 ctttgccatt cgctgattga taagacgcta ctcgatggct atctacacca gcaaatgcat
     2281 cgggatcggc agaaacaacg ctgatcctgc ttgctccttt ttcaagccat tcatccgctt
     2341 gatcgatttt agattgtggt acattctcaa tacgatccgt tgcagcatgt aaaagaaatt
     2401 ctctttggat ctggtcgtca gtccattgaa cgataacctc agcggctcct agtttataag
     2461 cttcttgtgt gatcaatcga gcaaggggtg cctgttccac gctgatctgt aaaacaactg
     2521 tgtgtccttt ttctgtggct acgccagttt ctgcaattaa gcgtgcatat ttttttagaa
     2581 gttcattaaa atcagataac ataataaaaa ttcctcctca ccattttttc tggtaactat
     2641 tagcatgata ccatttaatt atgattcaaa caatgaggct ttaaataaaa aaaggaggtt
     2701 atttttttaa ggttaagttt catgatatat gttactactt gctatttcag cttgtgaaga
     2761 aaatcaaaaa ctaattttgc atatcaaaaa aacatgttac aatgaaatta atcaatggtg
     2821 gaagcgttat aaaaaaatta aggagattaa aaaatatggc aagaaaaaag acgattacta
     2881 gagaacagat tttggcagct gcttatgaag tagctgccac cgatggattt acacgattta
     2941 ctgcgagaaa catcgcgaac aaaatgaatt gttccacgca accgatttat cttgagttta
     3001 aaaatatgga tgacttgaga caagctcttt ttgagcagat ctatgatcat ttacaatatg
     3061 aagtgttcac tgttgagcat acagggaata cgattatcga tcttgctttt aattatatcc
     3121 attttgcaag aagagagaag aagttgtatc gttcactgta cttagaagaa tatggtggag
     3181 gaaaagagat gcagaatttc tcttatactt actttgtgaa tacagtgaaa cgcgatccag
     3241 cttatgtgaa tctttcagaa gagcagatcg aatctttgta taatgggaca tggatcatag
     3301 ctactgggat cgcagctttg atgtcttcaa gcatcatcca tccaactgat gaacagattg
     3361 aaaagatgat ccaagacagt atagatgcta tcttgaaatt caaagaacca atcgaaatcg
     3421 attgaatata ctcaaaagtt ccacaaatca gtaggtaaca agagatgtgt caaaataagg
     3481 aaaagttcgt ttttcttaca gcgtatgcat ttttaactcc cgaagaactc ttccatgaat
     3541 gcaatgaata aaagagccat acagattgaa taaat
//
LOCUS       DAXWLG010000207         3547 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXWLG010000207 DAXWLG010000000
VERSION     DAXWLG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.214"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            121..2109
                     /gene="treP"
                     /locus_tag="ACGE4T_002562"
     CDS             121..2109
                     /gene="treP"
                     /locus_tag="ACGE4T_002562"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFF8387770.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            complement(2189..>3547)
                     /locus_tag="ACGE4T_002563"
     CDS             complement(2189..>3547)
                     /locus_tag="ACGE4T_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFF8387771.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT         1011 a          717 c          722 g         1097 t
ORIGIN      
        1 aaaacgatat cattttctct cgtacttttt aataaaacgc ttgcaactct ctccgaaaaa
       61 aagtatagta aatgaagaaa caacttgcat agacaagtta agagaaaggg gtttattgat
      121 atggcaaaat atcaagcaga tgccgagaag ctgttacatg atatcggcgg aaaagaaaat
      181 attgctgcag ttagtcattg tgcgactcgt atgcgttttg tcctgaatga tccaggaaaa
      241 gcggacgaaa aagcgatcga agatatacct agcgtaaaag ggatgttcac gaatgcagga
      301 cagttccaag tcatcatagg aaacgatgtt tctacttttt ataacgactt cgttgcagta
      361 tctggagttg aaggggtttc caaagaacaa ggaaaagcag ctgcaaaaca aaaccttcat
      421 cccgttcaac gtgctattgc cgttttagca gaaatcttta caccactgat tcctgcaatc
      481 attgtcggtg gattgatcct tggattcaga aatgtacttg aaggtatcca gtttgaaagt
      541 cttggtggaa caatcgttga acactcgaag ttttggaatg gtgtcaatgc ctttttgtgg
      601 ttaccgggtg aagcaatttt ccattttctt ccagtaggta tcacttggag tattgcaaaa
      661 aagatgggta ctacacaaat attaggaatc gttttaggaa ttacgttagt ttctccgcaa
      721 ttattaaatg cttatagtgt agcttccaca gccgcagcag atattccatt ttgggatttt
      781 ggttttgctc aagtccagat gattggttat caagcgcaag ttattccagc catgctggca
      841 gggtttatgt tagtgtatct agaaatcttc ttccgcaaat atatccctca gtcgatctcg
      901 atgatttttg ttccattgtt ttcattgtta ccaactgttt tagctgcaca cgttatttta
      961 ggtcctatcg gctggacagt cggaagctgg atctcaaata tcgtaaatac tggattgacg
     1021 tcttctatca gctggttgtt tagtgccgta ttcggattcc tgtatgctcc tttagtgatt
     1081 actggtctcc accatatgac caatgccatc gatatgcagc tgattgcaga tttcggatca
     1141 acgaatttat ggccgatgat tgctttatca aatatcgccc aaggctctgc agtcttagcc
     1201 atcatcttct tacatcgtgg gaataaaaaa gaagaacaaa tctcgattcc agcaatgatt
     1261 tcttgttact taggcgtaac tgaacctgct atgttcggaa tcaatttgaa atacgtctat
     1321 cctttcgtcg ctgcaatggt cggctctgga ttagctggca tgtttgctaa cttgatgggt
     1381 gttcgagcta acgcgatcgg agtaggcggt ttgccaggaa tactagctat ccaagcagaa
     1441 acatgggtac ctttcatcat cgcaatgatc attgctgtca tcattccatt tggcttgacg
     1501 atcatcttta gacgtcaggg aattttgaat aagattgatc ctgctgttcc tgaaaatgct
     1561 gctgatgtac aacttcaaac agcaaatggc gcaacagcaa cgccacagtc tttcgaacct
     1621 gtttcagcaa ctggtacggc agttgcaaca aaagaaacat tattcgcagt cgctgcaggg
     1681 accatcaagg aaataactga agtaaatgac ccagttttct cacaaaaaat gatgggtgac
     1741 ggttatgctg tcgaaccgtc aaacggcaaa gtctatgcac cagtaaacgg taaagtgacg
     1801 agtgtctttg aaacaaaaca tgccattggt attctttcta atgaggggct ggaagtactt
     1861 gttcacatgg gattagatac tgttgaacta aagggtgttc ctttcaatgt attcgtgaaa
     1921 gaaggatacc ttgtcacacc agagaccctg attgctgaaa tggatttgcc agaaatagaa
     1981 caagctggca agaaaactga cattattgtt gctttgacca ataatgaaaa agttgcagga
     2041 ctttctcttg accaatcagg acttgttcgc ccgggcgaag cagtcggtaa agctgaagta
     2101 aaatcttagt cagtaagcgc cccatagaac agtacctgta cgtcaaataa cccgacctat
     2161 agaatagaat cgggaatgtt tggcgttttt agttacgaga aaaatgttgt tgtacatcct
     2221 tgttccaagg caacagttga tctaaagttt ctgctgaaag tacgggaaga tttggtatct
     2281 tatcaaatag aaactctaga tactttacag gattcaatcc gttaagttct gctgttttat
     2341 atacactcag aatgattcct gaagttcgtg ctccttcaag acttgtggaa tgcaaccaat
     2401 ttttccgtcc tatcaccagc tctttgatat ttcgctccgc acgattattt gtgagttcta
     2461 aacgaccatc ttcaaaaata ggttcaaaac cacttcgtag tttacacgca tattgaacgg
     2521 ctgcatccaa cttggatttt gcaacaggat ctatcgttgc catccactca taaaaagcaa
     2581 taaataatgg acgcagttct tgttgccgac agcttaatcg cttctcagga gaaaagtctt
     2641 tccatttctt ttctaggaca aaccatttat ccaattgttt gactgcttga gctgcgggaa
     2701 tatccgtgtt tttctttcct tttggaattg cttcgaaaaa tttccgccga acatgcgcga
     2761 aacaatagac aagtcgtaca ctttccacgg cattataccc gctatagccg tcacagtgta
     2821 aataccctgt aaactccttg agaaaagttt tcggaacatc tccagcacga ctttcgtcgt
     2881 ggtgataata aacgatggga tgctgactgt cttttcctgt acaaaactgc cagtaatagg
     2941 tatctgtttt ggcactttca atcacacgat aacttgtttc atccccgtgt agaatctctt
     3001 cttgattaag atagactctt aatcgttctc ctaaagggtc taacgcgttt tgcactgctt
     3061 taatatgcca attggtgata ttatctctcg aaatatccaa cccatgtgtt tgtttccaat
     3121 agttctcttg ccgataggca ggaactttct gaccaaattt catccggatc gtttccgcca
     3181 ctaaactatt ggaacctaag ctattagaaa tcaaaggttt aggaacgatc gcttttttaa
     3241 aagaagaaag tccttctttt tcgcagcttt tacaacaata ggtatgttga taatgggtga
     3301 gacaatccaa catagcctga tggaaacgaa tttcttctcg tacttttgtc cgtccgatat
     3361 ctttcatctg tgaaccgcaa tgttcacaaa attgttcttc caaaggaagg agatgttctt
     3421 tctctgttat aggtaagtgt ttgattttgt ctgctttcgt tcctacaact cgttgatgtc
     3481 cttttactgg aataatggtg gcagctgctt ctggaacact tacagaaatt tcttcatcac
     3541 taaaaag
//
LOCUS       DAXWLG010000208         3523 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXWLG010000208 DAXWLG010000000
VERSION     DAXWLG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.215"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..913)
                     /locus_tag="ACGE4T_002564"
     CDS             complement(<1..913)
                     /locus_tag="ACGE4T_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFF8387772.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPY"
     gene            complement(885..2084)
                     /locus_tag="ACGE4T_002565"
     CDS             complement(885..2084)
                     /locus_tag="ACGE4T_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFF8387773.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(2214..>3523)
                     /locus_tag="ACGE4T_002566"
     CDS             complement(2214..>3523)
                     /locus_tag="ACGE4T_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8387774.1"
                     /translation="LSMSTLSLISLSIKDATDRASKETFANITNSFSMEINRQVNPGT
                     PRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFK
                     RTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKI
                     KSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNT
                     EDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSI
                     SNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIP
                     AFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLD
                     INVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT         1071 a          683 c          560 g         1209 t
ORIGIN      
        1 aataaggcaa acaaggtcga aggttcaaat gagtcaaaaa gtgaacaaac ttaggtgtct
       61 tatacccagc atcagcgaca acacaattga tttcaggaaa aagtctcttc gattgtttga
      121 cgagctgaaa ggcgacttga ctgtcatgaa tatttccagg agtgatgatt gtgctaagaa
      181 taaatccatt gtcgtcgcat gaagtatggg cagaataagc aaattgtttt tctcgttcgc
      241 ctttgacgta gtagccgctt tccggatcag ctttacttat ttttctttct ttcagcttca
      301 cttccttttc taagtccaaa gtaaaagggc gttttccagc ctctattcgt tgacgattga
      361 tctcatcttc taattcatcc tgataggctt tcgcttcatc atgggtcatt tctttcgtaa
      421 acttatgttt attggcgtta gctttgatgt gggtagaatc cagatacaaa ttatcctctg
      481 ttacaaaacc tgctttaact gcttgttcta aaatataggc aaaaatatcc tcaaacaccg
      541 tcgtttctct aaatcggcga acataattct ttccaaaagt tgagaaatgg gggattttct
      601 cctcgaagga gtagcctaaa aaccagcggt aagcgacatt ggtatcgact tccttaatgg
      661 tttgtcgcat ggaacggata ccgaataaat actgaataaa caccaatttg atcagaatca
      721 ctggatcgat acttgggcga cctaaatcgg agtaagtggc ttcgacgatt ggataaataa
      781 attcaaaatc aagcgcggca tcaatctttc gaacgagatg atcttgagga actagatcat
      841 caatcgtaag catcgcaaat tgatttctac cattcgtatt gtttttagac atcatagcta
      901 tcactccttg cattcgtttt cataaatcta ttataaaaga aaagactgta gacatcatcc
      961 ataaaatgga ctttgtctac agtctgcgca ttctcattag agaacactcc cttttttgtg
     1021 acttttctga agctagagtg aatactttca actgcattag tagtgtacat gatttttcta
     1081 atggcgctgc cataatcaaa gagttgttca acatgagaaa agtttcgttt gcatacatct
     1141 actgcaccag aataatgaga ccaccgattt tgaaaactgt cgaaggcagc gtgtgcagcg
     1201 tttaaagaag aagcactgta gaattttttc atgtctcgac agacttcctt ataatcctta
     1261 cttgggaggt atcgtaacgc atttcgaaca agatgaacaa tacagcgctg cacaatgaca
     1321 gaaggaaaaa ttgcttttgc gccttctttg agaccggaaa caccgtccat cgaaatgaaa
     1381 aaaacatctt ctacaccacg tactttcagt tcatcaaata cttgcatcca gcgattttta
     1441 gattctgttt gattgagcca taggcctaaa atctccttgt ttcctttaag atcatagcca
     1501 agaatggtgt acacagcata ttctttagct tcatagtttt cgcgtaaagt gacgtacata
     1561 caatcaacga atagaaatgc ataacacttg gctaatggcc gagcttgcca ttcttccaat
     1621 tcaggaagga cggcatccgt gatatcagaa atcatttcgt gggaaatatc aaagccgtaa
     1681 atatcttcaa cggttgcggc gatatcgcat tgactcattc ctcgtgcata catggaaaga
     1741 accttacctt cgatgtcgga gacatctcgt tttctattag gaatcaactc aggttcaaaa
     1801 gaagcctccc ggtctctggg aacatcaatg tttactttac caaagctcgt tttaagcgtc
     1861 ttattgccat aaccgtttcg tcgattgtca tgtttctttg gctctttaga atgggcgtca
     1921 taccctaaat ggttattcaa ttctccttga agcatttttt caaataaggg accaaacaca
     1981 tcttttaaaa catcttgcat atcctctaca gattcgggtt gataagtatt caaaatggat
     2041 tcagctaact tctcagcatc aggatttctt ttctttctag ccatcgtgta atcacctctt
     2101 gatcttattt tagaaaaata aaaaccgtga agcaaccacc cgcctaggat tgatggttac
     2161 ttacacggtt tatattacac tctcattttt ggtgctaaaa agcaccgaaa attttaatta
     2221 ttgtcaatta ataattcttt tggattcctt cttaatatat tgaatgaaga aataatcaat
     2281 gatacaagaa gcactaaact cataaatata actacataaa tcatgaattt aggtaataca
     2341 ttgatatcta aacttgataa tgtcttgtta aatccttcag cttctgcccc accacctaat
     2401 tggctagatg cagattgttt agctatttgt ttagctatat caccagtaac cttattcaat
     2461 atattattcc caagcttgtc agctgtatat tgagctaaga aataggaacc aacgaatgcg
     2521 gggattgata taaataccat ctcaattagg aattgaccaa aaatttctaa ttttgatata
     2581 cctaatgata gtaatactgc tatttctttc tttctagcat tcatccataa gaataataat
     2641 aatgaaacta caactcctgc aaatattaat gagccaaaaa ataatttatt tgaaattgag
     2701 taaatacctg atatagattg ttgtaatgca gggtaatttg atgaactttt aatcaaatta
     2761 tattctctcc aatttatatc taatttccca agatctttta ttacactatc aagattctta
     2821 tcacctttta caaagaatgt tgcatcttgg tatacagctg tatcttcggt attaccataa
     2881 actttagcag ctgtatgaat atcagtgatt aatgtatttt cgtatagttc ttgtgctgca
     2941 cttactccac cgctattatg cccatcgaat agacctttaa tttctacttc tactgtttca
     3001 tctgcacctt tctcattatc agcatcaaat aaattagatt ttatttttat cttatctcca
     3061 actttaaggt tatttttttc agctaaatct ttatgcatta agattttatt tttatcttca
     3121 ttttctaaat gctttccttc tactaattta tatgcttctg atacaaattt tgtttctttt
     3181 gttgagtcgt taaccccagt taacataact gttcttttaa aattctttgc tctttcaggt
     3241 gattgattcg caagagtctc ttgagtttca ataatatcat aatcaactaa atctgcaaca
     3301 ctgtttattc tttttacata ggagtctata ctatctgttt gagatatctt tttaatatct
     3361 tcacctttta catttccccc acctctaggt gttcctggat ttacttgtct atttatctcc
     3421 atagaaaaac tatttgttat attagcaaat gtttcttttg aagctctgtc cgtagcatct
     3481 ttaatagata aactaatgag acttaaagtt gacatagata aaa
//
LOCUS       DAXWLG010000209         3511 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXWLG010000209 DAXWLG010000000
VERSION     DAXWLG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.216"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..190)
                     /locus_tag="ACGE4T_002567"
     CDS             complement(<1..190)
                     /locus_tag="ACGE4T_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387775.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKL"
     gene            complement(488..1462)
                     /gene="sgrA"
                     /locus_tag="ACGE4T_002568"
     CDS             complement(488..1462)
                     /gene="sgrA"
                     /locus_tag="ACGE4T_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFF8387776.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1658..2497)
                     /locus_tag="ACGE4T_002569"
     CDS             complement(1658..2497)
                     /locus_tag="ACGE4T_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8387777.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2682..>3511
                     /locus_tag="ACGE4T_002570"
     CDS             2682..>3511
                     /locus_tag="ACGE4T_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFF8387778.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     V"
BASE COUNT         1165 a          727 c          518 g         1101 t
ORIGIN      
        1 ataatttata ttctggatac ctttcgcaaa acgattctgc ttgttgaatc accgaattca
       61 tcttagccag ggtgtagttt agatctaaag cgatatcctt caccacatat aatttctcgt
      121 ctctttcata acaatagttc aaaaattgat atacttgcct ttcattgata tcaataaatt
      181 cttcaaacaa agtacatact cctttttatc tattatttag acttattccg acagctacat
      241 gaataacgta caaatagatt cgttactgat ttttcagaga tactaccatg cgcagtgtgc
      301 attgtcaatt attcttgctc ttattttcca tttgtgttcc tgttatttcc tgtttctcaa
      361 atatgttagt aaacaaaaat agatctggca aagctacaac ttcgccaggc aatacagcac
      421 tctcgaatga aatattcact caaaaatcta tcgttttatt caattttaga tctgttgctt
      481 ttattcttta tttcactcga tggaagagag aacgaaaata gcttgctcct acaatcaaaa
      541 tgagtaacac acccatccat gtaattagct tgttcaaggt tctactacca gtttctggta
      601 aatcgctttt tctattatct gtttctttcg ctttttcttg atgatcaatt ccacgagaaa
      661 caaaatcagg ctgttttagc acacttttta ggttgctccc tgcatttctc ttttgttcct
      721 tagttggtat gataggtacc aaatcaagtt cattatttac ttttcctgtg ctgctttcgc
      781 tgctcgtgct tcctgtgcta ctctcactac tcatgcttcc tgtgctactt tcactactcg
      841 tactctccgt actgctttcg ctgctcgtgc ttcctgtgct actttcacta ctcgtactct
      901 ccgtactgct ttcgctgctc gtgttttctg tgctaatttc actgctcgta ctctctgtac
      961 tgctttcacc gctcgtgttt tctgtactgc tttcactact tgtgctatcc gtactgcttg
     1021 gcggtccaac aactgtccaa ctgcttggtt tcaaattctc cgtgatcgtc atctgtccat
     1081 cataaaaaac aatcaaatca actttatctt tactcttgat ctcatcaatc gatagttctg
     1141 cagaaatcat tattgtatac tcaataccag cggtccattg tacgtctttt aaataaactt
     1201 ccccaataac tttttctgaa cttccattac catcccaacc atatgttaga acagcatgta
     1261 catcactagc attcacgctt ttgtttggtt tgactggaat agtaatgact tgttgtgtca
     1321 tgtcataaac aatccgatca gttttcacag taaatgaaag attctcattt gcccgttcat
     1381 ttgcagaagt actgattcca atcattgctg caattatact cagcccaaga ataacaaata
     1441 atacggtcgc tgttttcttc atttctatca ctctccatat aattaccttc tatttgataa
     1501 aggaattata taaaatgaaa atgatgagaa cacgacagtc agttgtgaaa atctaacaaa
     1561 agatgtcgtg tattcgcttg aatttgttta ctttttcgtt taatcaatga cagacttaaa
     1621 aaagcaagac cgatatcagc cttgctttct tcatctttta ttgataaaca gcagtgtatt
     1681 tcttcatccg ctttgtttta tatgcataga gtaacaaacc aacaatcaag actaacaaga
     1741 ataatgtagt tgatatagat gcttcagccc caaagtttcc cccagtaatt attgtatttt
     1801 gcgattggat cagagtggag aaaaaacctt gttcttgcgt tccgctgact ggtataccca
     1861 aaatagctcc ttgtgtccaa ttccaagtaa tatgagcacc aatagccgta aataaacttc
     1921 ctgtactgta gataagtgcg ctgaaaaagc aacctgctaa aaagatattg aaaactgcca
     1981 gaaatgtcat gttaggattc aatccgtgca aaagagcaaa taatgcagca gatccaatca
     2041 ttgctattaa cggaccaaat ctaagcatct gatgctgaaa aaagccacgg aataaaagct
     2101 cttctgatgc ccccactgca ataaataaaa taaaactcgt tatcagcggc acaatgtttg
     2161 cgtttcctga atctaaccga tacgcccccg tcaaatataa tggactagta ataagcagaa
     2221 acactccaat tccaacaacg agacctcccg ctaaataccc ccagtctttt cctcgaacag
     2281 aaacaccata atcttctatt ctttttcctt cgaccttact agcaagaaat aaagctagta
     2341 aaaaaacagc taaatgtccg accgtaccgg caattgctcc aatccattca ctatttccaa
     2401 taaacatatc cgcaagtgca ttattgacaa gaattcctaa aacaaaacca acaaagacta
     2461 ttaaaacaga tccaatcaac cgaataatcc tgatcatctt tcatccctcc tttatattaa
     2521 gtttaacaac ccttttcttt cttgtctcgt gaaaactatg taaatgttct ccatcataga
     2581 gagaatagat ttttacgaac taagaatatc tacagcaacc ttacgaaaat gaaagactgt
     2641 ctccctaatt cttctgttag actgttttta ataaggaaag gatgggaaat tttttgaaaa
     2701 aacaactact aattatgtgc ctttcagcat gtgcaatttt actattctca ggttgtagca
     2761 agtccaatga aacgattgca acgtttaaag gtggaacact tacagttcag gatttttatg
     2821 ataacccaaa agttaaatca aataatcaaa cactattaaa aaaaatgatt gtcttcaaag
     2881 catttgatga tgtatacgga aaagagattt cttcaaagga agtaacaaaa gaatataatg
     2941 aacaaatcaa aaaactcggt ccaaactaca aggaacaatt aaaagcagtt ggccaaacag
     3001 aagagacata taagaaactc tttaaacaga tgctggcttt ccaatacggt ttgaaagcca
     3061 atgtaaaatt aacagataaa gatctagaca ctgcttggaa agaattctat ccagaagtta
     3121 gtacacagat cattctattt tcgactaaag aagaagcaga taaagcaaaa aaagaagcaa
     3181 atgaaggaga aaatttctcc aagcttgttc aagcttatgg aaaaaataaa acattaaagg
     3241 aaactgatgg aaaaatgaat tttgattcaa ccaatccaga gattcctact gaagtaaaaa
     3301 aagcagcatt taaactaaaa aacggggaag tttctgatat cattcctgta acagatccaa
     3361 cgacttatca gcaaagctat tatctagtaa aaatggtaaa aaaacaggat aaaggttcta
     3421 ataaagacaa atacaaatct gagctagaaa aaattgctac cgaagcaaaa actcttgata
     3481 cggaattcat ggataaaaca atccgtaaag t
//
LOCUS       DAXWLG010000210         3460 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXWLG010000210 DAXWLG010000000
VERSION     DAXWLG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.217"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..182)
                     /locus_tag="ACGE4T_002571"
     CDS             complement(<1..182)
                     /locus_tag="ACGE4T_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8387779.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACGE4T_002572"
     CDS             517..951
                     /locus_tag="ACGE4T_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8387780.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACGE4T_002573"
     CDS             974..1294
                     /locus_tag="ACGE4T_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387781.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACGE4T_002574"
     CDS             1435..1626
                     /locus_tag="ACGE4T_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387782.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACGE4T_002575"
     CDS             complement(1697..1954)
                     /locus_tag="ACGE4T_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFF8387783.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1981..2124
                     /locus_tag="ACGE4T_002576"
     CDS             1981..2124
                     /locus_tag="ACGE4T_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387784.1"
                     /translation="MIQNNTLCEMAQAVLWIYPVKSVSILKLEEKLAIIKVKEGVGFS
                     WIM"
     gene            2112..2375
                     /locus_tag="ACGE4T_002577"
     CDS             2112..2375
                     /locus_tag="ACGE4T_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387785.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACGE4T_002578"
     CDS             2399..2587
                     /locus_tag="ACGE4T_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387786.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACGE4T_002579"
     CDS             complement(2670..2855)
                     /locus_tag="ACGE4T_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387787.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACGE4T_002580"
     CDS             3070..3441
                     /locus_tag="ACGE4T_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8387788.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1293 a          493 c          697 g          977 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga
//
LOCUS       DAXWLG010000211         3451 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXWLG010000211 DAXWLG010000000
VERSION     DAXWLG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.218"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..2232)
                     /locus_tag="ACGE4T_002581"
     CDS             complement(<1..2232)
                     /locus_tag="ACGE4T_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8387789.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACGE4T_002582"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACGE4T_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          966 a          762 c          548 g         1175 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat a
//
LOCUS       DAXWLG010000212         3431 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXWLG010000212 DAXWLG010000000
VERSION     DAXWLG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.219"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..466)
                     /locus_tag="ACGE4T_002583"
     CDS             complement(<1..466)
                     /locus_tag="ACGE4T_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387790.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLL"
     gene            complement(606..1286)
                     /locus_tag="ACGE4T_002584"
                     /pseudo
     CDS             complement(606..1286)
                     /locus_tag="ACGE4T_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(1608..1829)
                     /locus_tag="ACGE4T_002585"
     CDS             complement(1608..1829)
                     /locus_tag="ACGE4T_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387791.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1927..2172)
                     /locus_tag="ACGE4T_002586"
     CDS             complement(1927..2172)
                     /locus_tag="ACGE4T_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387792.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2412..3026
                     /locus_tag="ACGE4T_002587"
                     /pseudo
     CDS             2412..3026
                     /locus_tag="ACGE4T_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1094 a          633 c          513 g         1191 t
ORIGIN      
        1 ttaataaaga tagaatcatc gtagttggat acgtagatga aacctcaact atagttcttt
       61 tccccgtacc tttaatagac actaaaccat tgtcattttc tatagtgctc tttttcccat
      121 ttactttcac aactgttccc caataattga atacaggtaa tgtgatattt tgctcatcgg
      181 catcttctac tgtatagact attttggaat tatatgcttt atgagatgca ttctcccacc
      241 gatcaccaac ttttacttca tgtttcttta aatgatcttt atatttcaat gcatctaatg
      301 gtgcatagtc atataggcct ccagttcttg aggatgtata acgtgtaaca tcttttttgg
      361 tcataactaa acgaccttta tacgactgaa ttttatgcga ataatttgct tctgttgctg
      421 cagtcacagt aaaaataata agaactattc ctacaaggct ccacatactt tttttgttgc
      481 ttgttttcca ctttaaaaac actatggtta agattaatga accaaaaagc acttgaaaac
      541 ccaaggttct cggttctgtt gcgaagtttg aaaatcaaac gcgccctctc tgaactccaa
      601 atatcttagg ctgttattcc cattaatacc ttgatttcag tagacaccga aaagccgaag
      661 agcgttccat ttcttcggtt ctttttatat attcctcgaa tggtctccat gcccttaatc
      721 gtggaagagg ctgtacggag actttgataa aatttatttc gtcgtttaat aggtcgatgg
      781 tcttgttcta ttaaattgtt aagatacttc acagttcggt gctctgtctt agtatataaa
      841 cccacactct gtaactttct aaaggcggag ccaagagaag gtgctttatc ggtcacaatt
      901 gctttcggct caccaaactg tttatggagt cgttttaaga aagcataggc tgcttgcgta
      961 tcccgtttct ttcgtaacca gatatctaag gttaagccgt ccgcatcaat tgcacgataa
     1021 agataatgcc aacgtccctt aattttgata taggtttcgt ccattttcca tgaatagaag
     1081 gattgtctat ttttcttctt ccaaagataa tagaggactt tgctgtactc ttatacccaa
     1141 cgataaatcg tagtatgaca aacatttatt ccacgatcat ataacaattc ctgaacttca
     1201 cgatagctta gattgtaacg caggtagtaa ccaacagcga caataatgac gtcttttttg
     1261 aattgtttgc ctttaaaatg attcattact ctgtcctctc tgtctttttt ctcaatttta
     1321 cactaaaata gattttttgg aaaactttgc aacagaaccg tttttactat atacttcttc
     1381 ccagaacttg tccgtctcct tcaaattatc tctacctctc gagttcattg ggtggtttgc
     1441 ccatttatta aacatggaat cacctttctc atactcatat atttatgtcc tatttcaatt
     1501 atatttatat atgcttaaat aaatttgtat cctgaaattt attgtaatat gaagctgtat
     1561 tttacatata gtaaccaaag agttaataaa ataaaaagag cgaaccatta tagatcagct
     1621 cttttttcat tgctgttttt aatcttatct agaatcactt caaatgtttc aaaggtattt
     1681 acatctaact gacataacca aagcaagtac ttcttttctt tgtaagtcca atcaatatta
     1741 cggtaagagg acattagatt atggtacctt gctctttgtt caactgaagc caattcccag
     1801 cgtttttcaa atattttttc ttcttccatt attgatccct cgcaagacgc tacctcaaac
     1861 ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa gtatagcaaa caaatatgaa
     1921 aaaaacttag aaggtagtaa aagttcttcc gtcttttgct ttgcttctat tcttaatgtg
     1981 tttcgttatc ggtgccctac aaagattttc attaattaca cactcattta ttgttgtgta
     2041 ttttctgaca tttctctctt gatcaattat tttaattcca tgggcatcct gatagttcat
     2101 aggagaggag cggctatcag aaatagttgc acctatctct tgaacttctt ttacaacttc
     2161 tggaaaatcc atccatcctg cttcagctgt caaaagtaat aatctataat atttatcttt
     2221 ccttttcaat ctaagaaatc gtcaccccat tctttttctt ctattataaa ctatttaact
     2281 tttagaattc aaaaaccgtt tgtctattta gagtttaccc taaatagaca aaaatataac
     2341 attgaaaaat ttagagttct aacgtatgat tagggtgtat tataaacgtt atagaaaagt
     2401 agggaataaa tttgaaaatt gcttatatta gagtttcatc catcgatcaa aatgaacaac
     2461 gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat ttttatcgaa aaacaatctg
     2521 gagcgacgat tagtcatcgg ccgattttcc aggaagcttt agattttgtc agagagcaag
     2581 atatttttat tgtagaagcc attgatcgac ttggacgcaa ttatgacgag attatcgatt
     2641 cagtgaatta tctaaagaag aagaatgtgc gacttattat caccagttta ccgatcatgg
     2701 ctgaagcaat tggaaatcct ctcctagata aattcatcaa agacttaatc atttaaatct
     2761 cagccatgat tgctgaacaa gaacgtactg aatcgaaacg acgccaggca caaggaatta
     2821 aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa gttatacagt gcagacacca
     2881 aagatcctca acgtcgcctt gtctatagga gtatcgttca ggatcttgag aatggggttg
     2941 ctatctcaaa gattgcaaca gactacaatg tgactaggca aacaatttac cgaataaaaa
     3001 aagaaatcga ccaactaatt gtatgatttc ttttttgaat cacgtaatta ttaattagct
     3061 ttgtcatcaa tacagacaat cattttagat tcatctagct aagttgatta catttatgtt
     3121 aaaaagaatt tagttaacat aaatataatt gatgcataaa tcgtccttat ttaaaggcat
     3181 ttaacattga caacttagta taatgtatct tatgtaaact aataagattt gaattttatt
     3241 ttatacacac tcgataatag atcaatttta tggtcccccg catttacgat tgcacaagag
     3301 aaacataaag tcgacctatt agcatttctt ttcaattcaa acattaaaaa agtttatacc
     3361 tctgctacta aaaaatgaaa ttatctattt aaaatttaaa acggtaaatc tgattcatcc
     3421 aaattggaaa a
//
LOCUS       DAXWLG010000213         3314 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXWLG010000213 DAXWLG010000000
VERSION     DAXWLG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.220"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..2109
                     /locus_tag="ACGE4T_002588"
     CDS             <1..2109
                     /locus_tag="ACGE4T_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFF8387793.1"
                     /translation="EKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLGY
                     LQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTAK
                     DLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQYS
                     AMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDRELAVVAERASEL
                     PGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYLE
                     KQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKRN
                     YQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDPG
                     SVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELSS
                     NTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESPG
                     ISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQPH
                     LVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAKP
                     LASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQDH
                     INLTIAKEIMDAYYDMFMAK"
     gene            complement(2293..3102)
                     /locus_tag="ACGE4T_002589"
     CDS             complement(2293..3102)
                     /locus_tag="ACGE4T_002589"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8387794.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         1089 a          611 c          691 g          923 t
ORIGIN      
        1 gaaaaaataa gaaaaggtgc acaagaacct aagaacaaaa cgtttcgccg gtcccatgtt
       61 ccttttcgct taaattttct ttttttcatt atatttacgt tatttgttgc gttgattgct
      121 agattgggtt atttgcagat cgtgaacggc gaagagatgg aggcgcgagt gaagtctact
      181 tctacgatta cggtccaaga aagtactcct cgcggaatga tctacgactc cacaggaaaa
      241 gctttagtta cgaataaagc gaatcaggcc atcacatata cgagaggaac acaagttacg
      301 gctaaagatt tgcttgaaat cgcaacgaag ctgaactcgc tgatcgatgt tccggtagat
      361 gaaaatctga cagatagaga taaaaaagat ttttggctgg ctgatcccaa acatttgaaa
      421 gaagccacaa aaagactttc ggcaaaagaa aagcaattag ggaacagcga acaatatagt
      481 gcgatggtca ataaagtcac agctgatgag attaatttaa acgaggagca attaaaaatt
      541 gctaccatct ttaaacggat gaatagtgca tatgaattaa acactgtcta tatcaaaaac
      601 tctggtgtta ctgatcgaga gttagctgtt gtggcagaac gcgcttctga acttccagga
      661 gtttcgactg gcacagactg gacacgagaa tataatgcag caagttcact gaaaagtatt
      721 cttggcagtg tgacgactga aaagcaagga cttccagcag atgaagcaga gaaataccta
      781 gcaaaaggtt attctcggaa tgaccgtgta ggacaaagtt atttggaaaa acaatatgaa
      841 gatgtcctgc aaggaaccaa aacccaatat gaagtttccc ttgataatga aggaaatgtt
      901 tcgaatcaaa aagaaatctt ctctggggaa aaaggttcta atttaatgtt atctatgaat
      961 gcagaattcc aatcaaaagt agaagaaatt ttgaaaagga attatcaaac attgatcaac
     1021 aacggcaaag cacagtattc gcctggtgct tatgcagtgg ctatgaatcc tcagacagga
     1081 gaagtgctag caatgactgg tttcagtcat gaacaaggtt ccaaagaaat caccgagaat
     1141 gctttaggca cgattactag cgcctttgat ccagggtctg tagtcaaggc tggtactttg
     1201 acagcaggct gggcaagtaa cgcgatcagc ggaaatcaag ttttgattga tgagccgatt
     1261 cgtctgcaag gtgcttcaga aaaaagctct gtctttaatc gaagcggaca agttgctctt
     1321 gatgcagtca aagcatcgga attgtcttct aatacgtata tgatcaaagt ggcgttaaag
     1381 atgttaggac tagactatac acctggaatg ggactaccta gtttagatga agaagctaaa
     1441 gcttatcagc aattgagaga ctctttcaaa gagtttggtc ttggtacaac gactgggatc
     1501 gatttgccga atgaatctcc tgggatctca cgttctgttg attatatgaa gaagttcaat
     1561 gcggataatg gcaaagaatg gtatgctcca ggtaacttta ccgatttggc atttggacag
     1621 tttgacacat atacaccaat ccaattagct caatatgctt caacagtcgc taatggcggg
     1681 aaacgagtac agccgcacct agtcaaagcg atctatggca atgacgaaaa tggaaacttg
     1741 ggtgaagtga agaaagaaat tggcacaaca gtagaaaata cggtcaatat ttctgcagaa
     1801 aatatggcga tcctccgtga aggattccac caagttgtcc atggtaccga tccttacaca
     1861 acagccaaac cattagccag tgcgaaaatg gacctttccg caaaaacagg gactgcagaa
     1921 acggttgcgg aaggtcatcc agatattgcg acagtcaata gtaatatcgt ggcatacgga
     1981 ccaacagaca atccggaaat tgccatcagt gttgtcttgc cgaacttatt agacgaacaa
     2041 gaccacataa acttgacgat tgctaaagaa ataatggacg cgtattatga tatgttcatg
     2101 gcaaaataaa aaagaggtcg tgaaaaagcc gcttagtttc gagtatgaag aaaaattttg
     2161 tgaaaatagc ttctcacatt tttgcttaaa tttgacttca taccgagaaa ctggcttttg
     2221 aacaccgttt actcggcaaa aaaggttgtg acaaaaattg tcacaacctt ttttgtggaa
     2281 tctaattctc tattatagtt taagtagttt ttccagataa acagctaaac cgtcttcctg
     2341 attgctcaaa ggagtgacat catttgcaat gcctttgagt tggtcagtac cgttagccat
     2401 agctactccc caacctgcat aatccaacaa ttccaaatca ttatgctcgt caccaaaggc
     2461 aatgacgtct ttttgatcga tagacaggta gttagcaatc tcttgcacgc cttttgcttt
     2521 ttggattcct tttgaaacaa tctctaagat ggcagtaggt ccaccccatg tccgaacatc
     2581 gacataactg ccaaattgac gtgttaattc agcagataca gctcctgcaa aacgtttgtc
     2641 tgttcggaca agcaaagatg taggatttgt cgtcaaattt ttaggagaaa gcaaattctt
     2701 ctctcctgga cgagaagaag caaaaatttt ttcatcaaag aaatcaaaac tgtcaataaa
     2761 aaaagtatca cgattttcag cagcgataaa atctaaattt agctgctttt tttgcgataa
     2821 gatttcaaaa gcgatttcac gattgatcaa cgtttctctt tcaccatccc agtgctgatt
     2881 agggatgtgt actaatgcgc cgttaaaatt aaccatcggt gttgttaatt ctaattgttg
     2941 gtaaaactga tggctcattc ggaatgggcg tcctgtagca atactgacat gatgaccggc
     3001 attgattgct ttttttagag tctcttttgt tttagatgta atcatcgaat cttgattgag
     3061 ggtagttccg tccagatcga tggcaattaa ttttttattc aataagggtc cttccttcct
     3121 caactagcat ttgttttagt ctagcatatt ttaggtaatt aacaagagca atcagctatt
     3181 cctaacaaaa actacagtta tggctatata aatagctaaa cataagctaa acacgaacgt
     3241 taagtattat tttcgctttt tttgttaaaa aaacgttgaa aagcgaatga aacgtctata
     3301 taatttgaaa ttgt
//
LOCUS       DAXWLG010000214         3280 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXWLG010000214 DAXWLG010000000
VERSION     DAXWLG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.221"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..358
                     /locus_tag="ACGE4T_002590"
     CDS             <1..358
                     /locus_tag="ACGE4T_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8387795.1"
                     /translation="SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGA
                     GMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVI
                     SWLVTLNVYKHSQRDF"
     gene            922..2184
                     /gene="mobV"
                     /locus_tag="ACGE4T_002591"
     CDS             922..2184
                     /gene="mobV"
                     /locus_tag="ACGE4T_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFF8387796.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            2429..>3280
                     /locus_tag="ACGE4T_002592"
     CDS             2429..>3280
                     /locus_tag="ACGE4T_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFF8387797.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1208 a          435 c          676 g          961 t
ORIGIN      
        1 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
       61 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
      121 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
      181 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
      241 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
      301 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
      361 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
      421 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
      481 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
      541 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
      601 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
      661 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
      721 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
      781 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
      841 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
      901 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
      961 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     1021 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     1081 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     1141 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     1201 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     1261 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     1321 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     1381 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     1441 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     1501 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     1561 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     1621 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     1681 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     1741 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     1801 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     1861 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     1921 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     1981 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     2041 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     2101 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     2161 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     2221 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     2281 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     2341 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     2401 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     2461 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     2521 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     2581 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     2641 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     2701 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     2761 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     2821 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     2881 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     2941 ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac
     3001 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     3061 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     3121 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     3181 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     3241 gttaatctcc tatggtggtt tgttaaaaga aatacataaa
//
LOCUS       DAXWLG010000215         3277 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXWLG010000215 DAXWLG010000000
VERSION     DAXWLG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.222"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(497..1111)
                     /locus_tag="ACGE4T_002593"
     CDS             complement(497..1111)
                     /locus_tag="ACGE4T_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8387798.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1717..2094)
                     /locus_tag="ACGE4T_002594"
     CDS             complement(1717..2094)
                     /locus_tag="ACGE4T_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387799.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2394..3275)
                     /gene="galU"
                     /locus_tag="ACGE4T_002595"
     CDS             complement(2394..3275)
                     /gene="galU"
                     /locus_tag="ACGE4T_002595"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8387800.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1004 a          668 c          499 g         1106 t
ORIGIN      
        1 atttcattgc aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt
       61 tgtgtatttt atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta
      121 aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga
      181 agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt
      241 ctctttttcc actacttttt atttttctga cactacatat tcttataact catttctcat
      301 aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga
      361 tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat
      421 cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt
      481 gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat
      541 acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat
      601 ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat
      661 cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat
      721 caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga
      781 tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag
      841 aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg
      901 gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta
      961 caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg
     1021 cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt
     1081 ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca
     1141 tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa
     1201 cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc
     1261 ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt
     1321 ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg
     1381 atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt
     1441 cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc
     1501 caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca
     1561 taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat
     1621 ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc
     1681 attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct
     1741 actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat
     1801 ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa
     1861 agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc
     1921 aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa
     1981 catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat
     2041 cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa
     2101 cacacacctt tttttataat taaaaaattc gcttttttat tctactatga atcttctggc
     2161 ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac
     2221 cattagcata atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca
     2281 cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc
     2341 tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt
     2401 tctgttctta gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc
     2461 gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg
     2521 tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt
     2581 gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat
     2641 attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt
     2701 tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga
     2761 tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa
     2821 gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc
     2881 aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc
     2941 acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc
     3001 gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat
     3061 tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag
     3121 atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc
     3181 acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc
     3241 ccagcggcag gaatgactgc ttttttgacc ttcactt
//
LOCUS       DAXWLG010000216         3261 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXWLG010000216 DAXWLG010000000
VERSION     DAXWLG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.223"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACGE4T_002596"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACGE4T_002596"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFF8387801.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACGE4T_002597"
     CDS             complement(2261..2512)
                     /locus_tag="ACGE4T_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFF8387802.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..>3261
                     /gene="lexA"
                     /locus_tag="ACGE4T_002598"
     CDS             2659..>3261
                     /gene="lexA"
                     /locus_tag="ACGE4T_002598"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFF8387803.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGL"
BASE COUNT          888 a          721 c          611 g         1041 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact t
//
LOCUS       DAXWLG010000217         3121 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXWLG010000217 DAXWLG010000000
VERSION     DAXWLG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.224"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            91..846
                     /gene="fabI"
                     /locus_tag="ACGE4T_002599"
     CDS             91..846
                     /gene="fabI"
                     /locus_tag="ACGE4T_002599"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFF8387804.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(956..2008)
                     /locus_tag="ACGE4T_002600"
     CDS             complement(956..2008)
                     /locus_tag="ACGE4T_002600"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFF8387805.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2134..3054
                     /locus_tag="ACGE4T_002601"
     CDS             2134..3054
                     /locus_tag="ACGE4T_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFF8387806.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          913 a          625 c          593 g          990 t
ORIGIN      
        1 aataattaag aaatcaagaa gttattttag ctattattct ctattttttt acgactatca
       61 aatttttatc agaaacaaag gagagtcatt atgtcatttc tagaaggaaa aaaaatcgtc
      121 attatgggcg tcgctaacaa aaaaagcatt gcttggggtt gtgctgaagc aatgttgaaa
      181 caaggggcag aaattatcta tacttaccaa aatgaacgaa tgaaaaaatc attagtcaaa
      241 ttggtcggcg aagaagcatt cactgtagaa tgtgatgttt ctagcgacga aagcatcgaa
      301 aaagcaatga atgccatcgg tgaatatgct ggggagatcc acggccttgt ccatgcagtt
      361 gcttacgcca acaaagaaga attgtccgga aatgtgtctg atatttccag agacggctat
      421 caattagctc aagatatcag cagctactct ttaattgctg tatcaaaata cgcacaaccg
      481 tttcttgcac caaacagcgg gatcgttagc atgacttatc taggttcaga acgcgcaatc
      541 ccgaattata acatgatggg tattgcgaaa gcttctcttg aagctgctat ccgttatttg
      601 gcagctgagt tctcgcctaa aggaattcgg gtcaatggaa tctcagctgg ggcaatcaaa
      661 acattagctg tcacaggtgt taaagactat caaaaactga tccagttatc tgaatctcgt
      721 acgcctgatc agcaaggtgt gacgatcgaa gaagtcggta atgcttgtgc tttcttaatc
      781 agcccacttt ccagcggaat catcggtgat attatctacg ttgacaaagg tgtccatctt
      841 tcataaactt ttgttgcatt ctttaaagta aaacatataa aaatcaggaa aaaacttttt
      901 aaagtctctt cctgattttt tctttttacg tttttttcaa ctcattactt tgaaattatt
      961 ccacgacttc aatccagcct tctggtgctt ctatgtctcc aaattgaata cctgtcaatt
     1021 catcatacaa ccgttttgtt actggtccta cttccgtttc actataaaat acatgaagat
     1081 tgttattgtg ataaatccca ccgattggag aaataacagc cgctgtacca catgcaccag
     1141 cttctgcaaa ttgatctaga tcatcaatga aaatatctcc ttcaattgct tgcagtccta
     1201 gtcgttcttc agctaactgc aacaaggaat atttcgtgat acttggtaag atagagggcg
     1261 attgcggagt gatgaatttt ccgtcttttg taattccaaa gaaatttgct gcacctactt
     1321 cttcgatttt cgtatgtgtg gctggatcaa gataaacaca atctgagtaa tccagttctt
     1381 ttgcttctgt tcctggaagt aaactcgctg cgtaatttcc gccgactttt gctgcaccag
     1441 tccccttagg agcagcacga tcgtaatcag atacaataaa attcgttggt tttagtccac
     1501 ctttgaaata cgctcctact ggcgtacaga agacgacaaa taaatattct ggagctggat
     1561 ggacaccaat gttttcaccg accccaatca gtaaagggcg caagtataat gtagcgcctg
     1621 ttccataggg cggcacaaaa tcttcatttg cttttaccac ttgcttgaca gcatcaacga
     1681 acatcttttc agggaccatt ggcatcagca atcgtctagc actttgattc atccgtcgac
     1741 tattttgttc aggtcgaaag agattgacct tgccgtcttt tcgacgataa gcttttagac
     1801 cttcaaaaca ttgctgacca taatgaagcg ctggagagcc ttcatgaatc gtcaggtaat
     1861 tatcttctgt caattctcct tcttcccatt gtccgtcttt ccatttggca ataaaacgcc
     1921 aaggagtttt gatataagaa aaacctagat tgttccaatc gatttgcata tacttaccac
     1981 cttcgttatt ttctccacat tttatcatta tccatattat ttgtcatttt attttcagct
     2041 tttttccttc attatagtta tttttgctat ttcctttttt gaaaacttct tggtacaata
     2101 cagaaagaat caaattttag aaaagaggat tttatgtttt cattattagc aactgccggt
     2161 acgacagact cttctggcga gacattgaca gatcaagctg tcagcaaagt aaatgcgttt
     2221 cagcggtttt gggacgggat tgattgggac gccattttgg ctgcctttat ccaaaaaagt
     2281 ttatatctga tttttttgat cgtccttttt tggattcttc accgtgtggg aaaatatctg
     2341 attgataagt cttttaatca gtattctaaa aaagccattc taaacgagag ccgcttaaag
     2401 actttacata gtcttttaaa tactattttt cattataccc ttggtttctt ttttatctat
     2461 gcaattttat ctgctatcgg tgtgccgatc ggatcacttt tggctggtgc cggaattgca
     2521 ggtgtggcaa tcggtcttgg tgcccaaggg ttcatgagtg atgttattac cggatttttt
     2581 attatcatgg aacaacagat ggatgttggg gactacatca agctagcaaa cttaacgatt
     2641 gaaggaaccg tcgtatctgt tggtatccgt acccttcagc tgaaagcaac ggatggtact
     2701 gtccatttca tcccgaatcg aaacatcacc acgatcagca atctctctcg tgcaaatatg
     2761 caagtacttt tagatattcg gattgttcct gaagaaggat atgacaaaat ctatgagatc
     2821 attgatcgag taaatcgtac attagctgaa aaataccaag aagagctcca aactgaacct
     2881 tctatctttg gattagtgga tcttggaaac agcaattttg cgattcgtac ggtctgctac
     2941 gcattgaacg gaaaacaata tgccttaaaa gaagagttct tgagctccta tgtcaaagaa
     3001 ttgacagctt ctggtttcac gattccgaac agtccaattg ctgtcggaaa ataatttgga
     3061 caaacgatgc aacgaatctc actaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     3121 c
//
LOCUS       DAXWLG010000218         3114 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXWLG010000218 DAXWLG010000000
VERSION     DAXWLG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.225"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..637
                     /locus_tag="ACGE4T_002602"
     CDS             <1..637
                     /locus_tag="ACGE4T_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387807.1"
                     /translation="NAVYAAEKEVISDPEQYASRYFADFEEEDKVLALPEGGFLKGEA
                     KIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKNDIETTPIMPRGAFPPDVGT
                     SGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFLRWKSYIDDGRVGNVNQAN
                     ATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNPVAGTYYVVQNI"
     gene            762..3113
                     /locus_tag="ACGE4T_002603"
     CDS             762..3113
                     /locus_tag="ACGE4T_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFF8387808.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1036 a          421 c          661 g          996 t
ORIGIN      
        1 aaatgcagta tatgctgctg agaaagaagt tatttcagat ccagagcagt acgcctcacg
       61 atactttgct gattttgaag aagaagataa agtacttgca ttgccagaag gtggtttttt
      121 aaaaggggaa gctaaaattg tatctatgga taatccagat ataattcaag gttcatataa
      181 ttcagaaact gatcctgctt cagtttcagt gacagaagca aaaaaaattg ttgctgaaaa
      241 attcaaaaat gatattgaaa ctactccaat tatgcctcga ggagcatttc cacccgatgt
      301 tggtactagc ggacaccttc ttgtactagc aggaggagga agctataaat cacagccatt
      361 tagtggtgcg ggatggcgtt ttggaggtat gggtgtaaaa ccagctgatg gtacaggcaa
      421 ctttttacgt tggaaatcct acattgacga tggaagagta ggaaatgtaa atcaagccaa
      481 cgcaactaaa tctggttcta ttcaaggaat ggcattgtat gctcgtgaag gattcaaata
      541 ctttgcacca aacgggcctg gttggacaat gaatataggt atttattaca cttataatcc
      601 tgttgcaggt acgtattatg ttgttcaaaa tatttaataa aacagcacaa agagatttct
      661 ttgtgctgtt ttattatttt tagttgttga caaacactaa aatataaaat ataatcaaca
      721 cataaacgaa cgtatgttcg gcaagaaagc aggggatttt tatggatgag atggttttaa
      781 gtactcagaa atggctaaac aagaaataca gtaatgttac agggtttgat aaggttccag
      841 aaaatgggcg aacagggtgg cctacaatct atggacttat tgaaggacta caagtagaac
      901 ttggtattac taatctagta gctaattttg gacctaccac tgaaaaaatg tacgataatc
      961 aggttactcc taaatggggc aaaaatctac ctaaaaatat tgtatttctt attcaaggtg
     1021 ccttttggtg taaggggatt aatccaggtg gcttcgatgg ggtatatact ccatatttag
     1081 atactgctgt aaaagagttg cagactgatg cagggtttaa tggaagcgct gtaactggag
     1141 ttttggatgc gaaatgggcc aaagcgttat ttgatatgag tgcttttgtt cttgttcccg
     1201 gcggagatag taaaattaga aaaatgcagc aatggttaaa tgtaaattat ttagaataca
     1261 cgggaataat gccttgtgat ggtatttatc aaagaaattc aaatcaagca ttaatttttg
     1321 cattacaagc tgaactagga tattctccca gtgaagcaac agggtcttat ggtaatggga
     1381 caactagtaa aacttatcct gtgtctgaag gtaactcggg aaactatgta agaataattc
     1441 aatatggatt atatgttaat ggttattttg aagacggtac attcgatggt attttcacta
     1501 aatatatggg tttagaagta ctggcattcc gaaaatttat ggtattgcct gaatatactg
     1561 aaatagcaga tgttgttgta ataaaaggac tattgtctag cgctggagat attagaagat
     1621 ctgcagatgg cgcagatact tctacgcaat taactagatc tcaaatccaa acattggttg
     1681 ataatgatat aaaaatagtt ggtagatatc ttactggtac tgttggaatt ggaaaagatg
     1741 aacgaaataa gtatctaact acagaggaat tgaataatat tttttctaaa aatttatctg
     1801 tgtttcctat ctatcaagat ggaggtgcag cattagctta ttttacttat gatcgtggct
     1861 tatcagatgg taagaaggct atagatgcag caaaaaatct aaatatacca ttaacaacag
     1921 ttatttattt tgctgttgat ctagacatgt taggggaaga aattcttgca tatgctggag
     1981 aatattttaa gggggtatct gctgtaatgt catatagcgg gtatcaaacg ggtgtttacg
     2041 gaacaagaaa tgtttgttct caaataatca ataacgggta tgcttcgttt gcatttgttt
     2101 cagatatgtc gactggttat agtgggaatt taggttttcc tatgcctaga caatggtctt
     2161 ttgatcaatt tgttgaattt accataggaa gtggcaacgg agctgttggt atcgatttaa
     2221 tcgctacatc gggtagagat agcggattta atgagttatc aaatgatact aataatgata
     2281 attacattgc caaatacaat cagaaggtta taaatcagat ggtacgtgcg tatcaatatc
     2341 tatcacaagc tggtcttgat aatccttgga accctcattt gacattttat agatatgtaa
     2401 attattctgg tttgtcatgg gacatcattt cttctcctgt aacagagcat gatagaaaaa
     2461 tttatgatga atataggaat aaattaacta atagcgaggg cctgtataat tattttatag
     2521 atcctaatac tggttcaata ataggtttgc ctcacttaat tgtgacgtta caatcacaga
     2581 tgtttctagc cgacacaatc aatgatagtg tttctgactt tgcaggttgg ttaggagatt
     2641 taatgacttg ctggggtgaa gttaagaaat taggaataca aatggagcaa ggtgtatttg
     2701 cattagtagg tacggctgca ggtggaacat ttagtctaga agatttatac caagatgttg
     2761 acgcggtaaa tgtttatacc ttagcaaaaa gaaattctga ttcaacagct ttatcggctt
     2821 taaaaacgta ctattgtgat aagggtgcta ttgatagatt taatcaattt gttaagaaca
     2881 gatttggttc ttcagataaa atcgcttcag taacaaatag tttattagaa aatgacgaaa
     2941 cagttgatat cttaggtgta gggataattt catttttcat tgataaaata gttaataatt
     3001 cacttgggac agattttact tatcttgatt ttattggaga tgaggggaaa gttgcaaatg
     3061 gattttctga aaaaattatt caatttcaag aaaatagaag taaacttaat taag
//
LOCUS       DAXWLG010000219         3103 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXWLG010000219 DAXWLG010000000
VERSION     DAXWLG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.226"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..1151
                     /locus_tag="ACGE4T_002604"
     CDS             <1..1151
                     /locus_tag="ACGE4T_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8387809.1"
                     /translation="KTQAALDMMNDHQPKTEILKKTGATVGVYDVNFDVKEGEIFVIM
                     GLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREVRRHKINMVFQNFGLF
                     PHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQYPSQLSGGMQQRVGLA
                     RALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTIIFITHDLNEALRIGDR
                     IALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQNIMVPALTTNIESDG
                     PNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRPLIDFLDENVTVIGKD
                     MVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSEVSEHE"
     gene            1144..2871
                     /locus_tag="ACGE4T_002605"
     CDS             1144..2871
                     /locus_tag="ACGE4T_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFF8387810.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         1031 a          543 c          708 g          821 t
ORIGIN      
        1 aaaagacaca agccgcttta gatatgatga atgatcacca gccgaaaacg gagattttaa
       61 agaaaaccgg tgcaacggtt ggtgtgtatg acgtgaattt tgacgtaaaa gaaggagaaa
      121 tcttcgtgat catggggctt tccgggagtg ggaaatccac attgatccgt ctattgaatc
      181 gtctgattga gccaacttcg ggcagtatct atattgatgg agaagatgta tcgaagttaa
      241 gcaaagaaga attgcgagaa gtccgtcgtc ataaaatcaa tatggttttc caaaacttcg
      301 gtctgtttcc acatcgaacg atcttggaaa atacagaata cgggttagaa gtccgtggtg
      361 tacctaaaga agaacgacaa gaaaaagcag aaaaagcatt agaaaattct agtttgcttt
      421 catttaaaga ccaatatcct agtcagttat ctggtgggat gcaacaacgg gttggcctag
      481 ccagagcttt agcaaatgat cctgagatct tgctgatgga cgaagcgttc tcggctcttg
      541 atccgctgat ccggcgagaa atgcaagacg aattactgga cctgcaagcc aatgttcaaa
      601 aaacgattat tttcattacc catgatttga atgaagcttt acggattggg gacagaatcg
      661 ccttgatgaa agacggcgaa atcatgcaga tcggaacagg tgaagaaata ttgaccaatc
      721 cagcaaatga ttatgtacgt gaatttgtgg aagaagtcga tcgttccaaa gtgcttacgg
      781 ctcaaaacat catggtacct gcattgacga cgaatatcga atctgatgga ccaaacgttg
      841 cattgacaag gatgcgtaat gaagaagtca gcatgctgat ggctgttgac agaaagagac
      901 acctgaaagg gatcatcact gcagatcaag cgttagaagc taggaagcaa aaacgtccat
      961 tgatcgactt cctagatgaa aatgtgacgg tgattgggaa agacatggtg gtaagtgata
     1021 ttttcaatat tatttatgac tcaccgactc cacttgcagt tgtagaggat ggaaaattaa
     1081 aaggtgtcgt catccgaggt agtgtgattg aagcattagc agaaacaagc gaggtgagtg
     1141 aacatgaata atctattgaa ctatcaatta ccagtggcaa actgggtaga aaatatcaca
     1201 gaatggttta cgacaacttt ttctggccta ttttcatttt tacaaacgat tggacaggca
     1261 gtgatgtcgg ggattacaaa tttactgtta gtgattccag ctcctttgtt tatcttgtta
     1321 ttgacggtag cagctttctt tatttctaag aaacgtccag gacttacgtt gttcacatta
     1381 atcggtctat ggtttattta taaccaaggt ctatggaatg atttgatgaa tactgtcaca
     1441 cttgttcttt tatctagtgt gatctcgatc atcattgggg taccattagg gatcttgatg
     1501 gcaaaaagca gtaaagccca aagtattatc aaaccgatcc ttgactttat gcagacaatg
     1561 ccaggattcg tttatttgat tccagcagtt gcatttttcg gtatcgggat ggttccaggg
     1621 gtatttgcct cagtcatctt tgctctgcca ccaaccgttc gttttacgaa tttagggatt
     1681 cgacaagttc ctaaagaatt agtagaagct tctgattcat tcgggagcac aagtcgtcaa
     1741 aaactattca aactagaatt gcctttagct aaaagtacga tcatggcagg gatcaatcaa
     1801 acaacaatgc tgtcactttc aatggtcgtt attgcctcta tgatcggtgc acccggctta
     1861 ggacgcggcg tattatcagc attgcaaaga gcacaagtcg ggaatggatt tgtcaacggg
     1921 gttgcattag ttatcttagc aatcatcatt gaccgtttta cacagcattt gaaccagcca
     1981 aacaataaaa aagctgcagg agcaacaaca cagaaaagta aaaaacgtca aggactgatc
     2041 attggtgctg tagtagtagt gatcttaggt gcgattggta tagggagttt ttcatctgcc
     2101 aaagaaacaa aacgtatcaa cttatcttat gttgaatggg atacagaagt tgcttcaaca
     2161 aatgtagtcg gtgaagtatt gaaacaaatg ggctatgatg taacgatgac gccattagat
     2221 aattcaatca tgtggaaatc tgtttcaaat ggcgagtcag atgccatggt ttcagcttgg
     2281 ctacctaaaa ctcatggttc acaatatgca caatataaag atcaagtcga agacctaggt
     2341 gccaatttga caggagcaaa agttggttta gcagtgccag cctacatgga tgtaaattcg
     2401 atcgatgagt taaccgatca ggcaggtaag aaaatcatag gaatcgagcc aggagctggt
     2461 gtagttacgg ctgctgaaaa cacgatccaa aaatatgaca acttgaaaga ctggaaagta
     2521 gagacatctt cttcaggagc aatgaccgtt gcactaggtc aagcgatcaa aaaacacgaa
     2581 ccaattgtag ttacaggatg gacacctcat tggatgtttg ccaaatatga tctgaaatac
     2641 ttagaagatc ctgaaaacgg aatgggaagc gaagagcaga tccatacgat ggttcgaaaa
     2701 ggattgaaag aagaccagcc agaagcttac aaagtattag acaatttcca ttggtcagaa
     2761 aaagatatgg aaaaagttat gttggaaatc aacaatggaa aagatccaca gcaagcagca
     2821 aaagactgga tcaaagagaa ccaagaatta gtcgaaagct ggaaaaaata aaaagacaga
     2881 cacgaaataa atggagcttc caaaattgga tcaaatatcc aaatttggaa gctccatgtt
     2941 cgtctctatc ttttttgtct ataaaaaaag agaatgcgta gctcatcttc gaaacgttca
     3001 aagacgccga caaactatta tgaatttatg ggtgcgagtc aaagagatcg atcgtaggta
     3061 tgaaagacta atgtgattaa agtttattat gtacgaagtg cat
//
LOCUS       DAXWLG010000220         2973 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXWLG010000220 DAXWLG010000000
VERSION     DAXWLG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.227"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            92..472
                     /locus_tag="ACGE4T_002606"
     CDS             92..472
                     /locus_tag="ACGE4T_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFF8387811.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            454..1368
                     /locus_tag="ACGE4T_002607"
     CDS             454..1368
                     /locus_tag="ACGE4T_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFF8387812.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            1328..1606
                     /locus_tag="ACGE4T_002608"
     CDS             1328..1606
                     /locus_tag="ACGE4T_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387813.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            1608..2297
                     /locus_tag="ACGE4T_002609"
     CDS             1608..2297
                     /locus_tag="ACGE4T_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387814.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2417..2503)
                     /locus_tag="ACGE4T_002610"
     CDS             complement(2417..2503)
                     /locus_tag="ACGE4T_002610"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8387815.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
BASE COUNT         1067 a          567 c          577 g          762 t
ORIGIN      
        1 cccttttagt gagctttact cattgtacct ttttaggtac aatggtattg cgaacgatac
       61 gtccgtagga cgtactgaaa gggtgggact tatgagcgaa caaaatcaaa atttggctag
      121 cgatcattct aaaaaatata cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag
      181 cgaatctgaa tttgcaaagc tgaagcagtc agctgaagct ttgcaaatga gtgtgccggc
      241 tttcgtaaaa gccaaggcac aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat
      301 tgcccaggca atcgcgcgcg atttagccaa agctggaggg aatatcaatc aaattgctaa
      361 gtggtgtaat acacaccaac acgacgttgc tcctggagac gcccaacgct tatccgaaaa
      421 cctaaaaatc atgcaaaagg agctacaaaa gatatggcaa cagttaaagt aagccgaacc
      481 acttcttgta gtcgtgcaat taattacgct gaaccacgag caaccattaa aacaggaata
      541 aactgtgacg tcgattatgc caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa
      601 gacgaccgtg tacaggcaca cttattgatt caatcttttc gaccaggaga aattaccgct
      661 gaaaaagcta atcaactagg ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata
      721 gctatttaca cacatacaga caaagaccat attcataatc acattgtcat taactccgtt
      781 aatctggaga ctggaaaaaa atttcaagct catggtcaag catttctaaa caaatgttat
      841 gacctaaatg acgagatttg tcttgcacat aacttaccaa tcactgagcg aggaaagaaa
      901 ccagaaaaac gaaccatgtc tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa
      961 gacgaaattc ggattgcgat cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac
     1021 caattttgta attccctcaa aacattgggg attcattgct tcaatcgcgg gaaaaatttc
     1081 acttacgaat tgatccacaa aaagaaaaaa gtacgatcca acagactagg aaaggactac
     1141 gaaaaggaga cgattttaca tgagctggac agacgagaac gaacctataa ccaacagcga
     1201 accaacgagc tacgaagagc tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga
     1261 gaacaacgaa ctcttacgcc tgaacaaccg gaaattagac cggctcgaag aaaccatgaa
     1321 caacacgttg aaagaggaac agaccaccaa caagaattat cgctctaaaa cgttaaaaga
     1381 catagaaaac gaaataaaga aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat
     1441 acaaactacg attgatttac aagttaaaaa actccatcaa gagctattag accagcgaaa
     1501 taaggaacag aaaaaggtgc ttattcaacg atcattggat tttcttgccc ccttatgttg
     1561 cttagtcctg acactgttcg cattttggtt agtatgggga ggctaaaatg gcattttatt
     1621 taccctatct actcattttt gttagtattt caggaagtat ttggctcatc tataaaattt
     1681 ttcaaacaag atattcatta aaaggctcta aaattcgatt taagcgattt ttctttcttg
     1741 gctgtatatt ttccttgata atcgtttcaa gcggcttatt cggcgtttta gagggaaata
     1801 agcgcgtttc tagaagtatt ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg
     1861 ataaaaagaa aaaagagcaa gtattagcac aaaaaataga aaaatttacc gcttgttatg
     1921 aggacatgaa tgatattttt gtgaagcaag aaaagcgttt gacggataaa aatatggaaa
     1981 cactcactcg actttatcgg aatttgccag aagaacagca aaaagagtac caagaaaaat
     2041 atgaacaagt aaaaaaagac gtgcaatatg tgaaagatac gaaagtggaa gaagcttgta
     2101 gtgatttatt tggagataca attccttttt caactagcga acaagaacga aaagaacgac
     2161 aacaatcagt cacatatgaa cgctatgaaa tcttgcttca acaagcaatt aacatacaaa
     2221 atctgactaa aaaagaaaca gctttaaatt atttaaaaag cgtcaaggaa tggttagatc
     2281 agcaacagca aaattgaatt tgattgaaat tcatgttaaa ctaaacatgc aaatgttcaa
     2341 acattagata cacgaaagcc ccagagaggt cggattctct ggggcttttt ttaggttgat
     2401 gtcgccttta ctgattttac ttcttgtttc ggttattcaa ccaataactg aatacagaga
     2461 tacaaactcc tacaagaagg gggcaaataa acagttcaaa catcagatac acctccttcc
     2521 agtcgcaaga ctgtcggata ggcgacaagt tattataccc tacaaaatta aatctgtgtg
     2581 gcaattttaa gtatctctta tttccgggtt gttagggctt gccctgaccg cctgtagggc
     2641 gctttaaatg ccagataaag gtttttatac taagactgtc ttaaaacggc ttagacaagc
     2701 tcttagagcc tcaaccctcc tttttgaaca atggttttag caaaggagct ggaggcgact
     2761 gcgcaggtat aatgtgagag cgtagcgatc acaccttttt ggcgaccgta gggagcgagg
     2821 ttttgaatta gcagtttaga aaagttcgcg aacctgttta aagaaaaaat aattgttttt
     2881 ttaggtggga aattttgtct ccctctttta taatcttttt ttaaacctct ttttaaacct
     2941 ctttaccgtc ggctgtgtcc ttactccccc aag
//
LOCUS       DAXWLG010000221         2964 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXWLG010000221 DAXWLG010000000
VERSION     DAXWLG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.228"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(63..605)
                     /locus_tag="ACGE4T_002611"
     CDS             complement(63..605)
                     /locus_tag="ACGE4T_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFF8387816.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            740..1129
                     /locus_tag="ACGE4T_002612"
     CDS             740..1129
                     /locus_tag="ACGE4T_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387817.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1133..1897
                     /locus_tag="ACGE4T_002613"
     CDS             1133..1897
                     /locus_tag="ACGE4T_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8387818.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2026..>2964
                     /locus_tag="ACGE4T_002614"
     CDS             2026..>2964
                     /locus_tag="ACGE4T_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8387819.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRET"
BASE COUNT          948 a          499 c          612 g          905 t
ORIGIN      
        1 aataaaaact gtctaacatt taaaatttaa tttagatatt agacagtttt taagtttgtg
       61 agttattttc cttgtcgttt tctcactttt ttagtccaga aaccacctga aatatatttt
      121 ggctcataag aaatgataaa agcacgctct tcgatttctt tgatcaggcg atacagttca
      181 cgttcgcttt ttctaggaga aagtatttcg agaactagcc gctcaccttc cagccccatt
      241 gcagaagtct gtgtcacacc ataaccgtgt tctctcagta tacccggcaa acttttatct
      301 tctgcgctat ttgctggcaa aatcgttgtg accatgatgt atcctaatgc caatttgtct
      361 tcaatcaaaa ttccgacact gatacctact gcgtaaccta acgcatagac aaataaattc
      421 agtggattat ctaaacgatc taaaacaagg gataagccga cgatataaat cgtgatctcg
      481 accatactga caagtggagc aatcagtcga tagcctttca ttgtcaacat gaaacgtaac
      541 gtgttcagag tgatataagc aaaattaatg aggaagatca tccccaacat ttttggatca
      601 atcatttgta aaactccttt catttatata acaacaatat caaaaaaaat cgtaagtgaa
      661 gaaaaattga aactttatac atacaaaaaa atgataaaat gattataata gaattataaa
      721 gataggttag gtgattaaaa tgaaaacggt caatcgtgtg ttttgtgcca gccacgtgct
      781 aagtctcctt ctcagtatat acttaggtgt ctattcgatt caacagccaa ttgaggtaga
      841 gaacattgtt tttcagatgt cgctgacaga tggtttaatt ttaagcgttt ttttcttttt
      901 gatactgttt attggtaata ttttcggtag cgttgccagt tttttgaatt tttctattta
      961 tccactcctt tcgctagcat taggagtggt gggactttgt tctctgtgct tatttcccga
     1021 gccattcagt cctgcattta tattatttgg tacactgaac ctgttccaga caactgttgg
     1081 agcatggttg ctatggcgat caggaaattt aatgaaaata ggtgagtaac aattgaatcc
     1141 aattatattg aaacgttctg tccatcatat tccagtgtta gaagttgttc tgaaagaatt
     1201 aagagaggaa cgtattcccg ttgtcatcta ttatcacgga tggcagacaa gtaaggagtt
     1261 agttttgacg caaggaagaa aaattgcgca gcagggcatt cgtgtgatcc tgccagatgc
     1321 gatgaatcat ggggagcgca aacagccggt ttcttctatt ccatcctata cattttggga
     1381 tagtatctac ggaaatctgt ttgaatttga cacacttatc aagcatttag aaaaaagagg
     1441 tcttctacat gatcagcttg cagttggcgg ggtttcgatg gggggaatga caacatgcgc
     1501 cttgttggct aaacatcctc atatagtagc gggcgtgtgt ttgatgggtt cccctgcacc
     1561 tttggaatat ggaaaagaaa tcgtaaggcg cgcatcggaa tataattatg tcgtaccaaa
     1621 agactatttt gatttgattt catgggttaa gagctatgat ttgtcattgc agccggaaaa
     1681 gttagctgga cgtcctctgt tcttttggca tggacaagct gacgaaaaaa tcccttatat
     1741 acaaacggaa cgatttgtag aaaaaaatcg gcataagtct tacggaaaag aaattgtttt
     1801 tcgttcctca aaaacacagc gacatatggt acaaatacca ctaatggaag aagcttccac
     1861 ttttttagca aaaaatttga taaaaaagta taaataattg aaacggaaaa tcactcatga
     1921 cgatagagga ttttccgttt tttattttgg gctataataa actctgttag gaaaatttgg
     1981 tataattgat agtaagaatg acaacatgtt agaaaggaag agagcatgcc cactttagca
     2041 gatgttgcaa aacgtgccaa tgtttcaaaa atgacggtct ctcgagtcat caatcatccg
     2101 gaacaggtga ctgacgaatt gaaagagtta gttttctcag caatggctga actggattac
     2161 cgtccaaata ttgctgcaaa agcacttgtc acgaatcggt cgcagatcat caaattattt
     2221 atactggaag aaatcgacac aactgagccc tattacatga atttgctgat gggaatcgct
     2281 aaacggatcg gtaaagcgca ttattctctc caattagtta caaatgatgc cttcgacgtc
     2341 ggttcttgtg atggatacat catcacaggt gtcagaaaaa aagacttcga ttggatcgac
     2401 cgtttggaaa aaccagttgt cttgtttggg gaaaattctg aaggatatga ttatgtagac
     2461 agcgataatg aatatggtac agcaaaagca acgcaatatg ccttagaaag agattatcaa
     2521 acgattattt atatcggtat cgaagtggac gagccttttg aaatttcaag ggaaactggt
     2581 tatttatctg tgatgaagga agttgatctt cctagtaaaa tcttgcggtt tccaaatcat
     2641 tcgacagaag tagaaagata tatgacagaa aactgggcag attttacaga taaaacttgt
     2701 tttgtatgta gttcagatcg gctagcacta ggagcagtaa gagggattat aaatgctgga
     2761 ggacacttgc ccgaagaatt tggtgtgatt ggatttgatg gggtattttt agaccaaatc
     2821 agttcaccta aattgacaac gatcaagcaa gatattatac gtatgggtga agtatgtgga
     2881 gaaatgttgt taaaaaaaat agaagaaaaa ggggcgagtc aaggttatcg tcgttttttt
     2941 cctgaactag ttatcagaga gaca
//
LOCUS       DAXWLG010000222         2816 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXWLG010000222 DAXWLG010000000
VERSION     DAXWLG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.229"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            46..1692
                     /locus_tag="ACGE4T_002615"
     CDS             46..1692
                     /locus_tag="ACGE4T_002615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFF8387820.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1683..2765
                     /gene="uxuA"
                     /locus_tag="ACGE4T_002616"
     CDS             1683..2765
                     /gene="uxuA"
                     /locus_tag="ACGE4T_002616"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8387821.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT          937 a          490 c          589 g          800 t
ORIGIN      
        1 ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat tgatttaaac
       61 aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc tcattttgac
      121 cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt cggtggcgga
      181 aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct aggcgaattg
      241 tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag ggcttaccaa
      301 gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt
      361 atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga acactttgat
      421 aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc tattactgaa
      481 aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc agtcgatata
      541 aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgcgct actttataaa
      601 cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt ttcacaaaac
      661 ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat
      721 gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt tccatggacg
      781 atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag
      841 ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc tccatttgtc
      901 aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa
      961 ttcgaaaaag ctggagtatt cttgactgat cgtgcaacgg tagataaagc agatacgatg
     1021 aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg ttgtttactg
     1081 gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct agtttctgaa
     1141 atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga tccaaagatc
     1201 tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctga tatgcctcag
     1261 cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa
     1321 tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt gactctcgct
     1381 gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat cccgagtcca
     1441 gacccactat tgacagactt tcagcataag ctttccgata ttcaactggg tacgcaagat
     1501 acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat ttttggtgta
     1561 gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa gatggtagca
     1621 ggaccgaatg ccgtacgaaa aacattacag gattacttac aagaacatgg aggaaattta
     1681 tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc ttagatgaaa
     1741 tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata ccagtaggag
     1801 aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct cgtgggttaa
     1861 cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga ttaccttccc
     1921 gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa tatggaatag
     1981 aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac cttgactacc
     2041 gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca gatgatccaa
     2101 gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tgaattgccg ggatgggaac
     2161 ctgagcgctt agctcatatc aaatccttgc ttgaagccta tgcatcagta gacgaagaaa
     2221 aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt gaagaggtag
     2281 gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga ttacctcgaa
     2341 tcgtaaagaa tcgagaagat ttagattggt tgtgccgcat agtagacagc ccttataacg
     2401 gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta tatgggattt
     2461 tagcagaatt ttgtcaaaga gatcgaatcc catttgcaca tgttcgaaat attaaatttt
     2521 tagctaatcg agatttctat gaggcaccgc ataaatccga atacggttca ttagacatgt
     2581 accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt cctgatcatg
     2641 gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat cgagcattag
     2701 gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa agtaaagata
     2761 attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa attctt
//
LOCUS       DAXWLG010000223         2791 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXWLG010000223 DAXWLG010000000
VERSION     DAXWLG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.230"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            86..2527
                     /locus_tag="ACGE4T_002617"
     CDS             86..2527
                     /locus_tag="ACGE4T_002617"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFF8387822.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          926 a          486 c          652 g          727 t
ORIGIN      
        1 atttaatctt agaaaaaaat aactggaaca agagctattt gtttttacag caaagaatgg
       61 tatatttttg taggaggtgg cttacttgaa agaagtcgtt atgatagatg ctgcaagaac
      121 gccgatagga aagtatcgcg gaagtttaag tccatttact gcagttgaat taggcacact
      181 tgtgactaaa ggattattag ataaaacaaa actgaaaaaa gataaaatcg atcaagtaat
      241 atttggaaat gtcctgcaag ctggtaatgg tcaaaatgtt gccagacaga ttgctttgaa
      301 cagcggtctc cctgtagatg tcccagcaat gacgatcaat gaagtttgcg gatctggtat
      361 gaaagcagtg atcttagcac gccagctgat ccaattaggt gaagcagaac ttgtgatagc
      421 cggcggaaca gagagtatgt ctcaagcacc tatgctgaaa cagtatcagt cagaaacaaa
      481 tgaatatggt gaaccaattt ccagtatggt caacgacgga ttgactgacg cattttcaaa
      541 tgcacatatg ggattaaccg cagagaatgt tgcaacacaa ttttctgtga gcagagaaga
      601 gcaggatcgc tatgccttgt cgtcccagtt gaaagcagca catgctgtcg aagccggtgt
      661 attttctgag gagatcatcc cagtcaagat ttctgatgaa gacgtgttat ctgaggatga
      721 agcagttcgt ggaaatagta cattggaaaa actgggcacg ttacgtacag tattctcaga
      781 agaaggaact gtaacagcag gaaatgcttc cccgttgaat gacggtgcct ctgtggtgat
      841 ccttgcatcc aaagaatacg cagaaaataa taatctgcct tatttagcaa ccatcaaaga
      901 agtagcggag gtcggtattg acccaagtat catgggaatc gctccaatca aagccattca
      961 aaaattgaca gatagatcag gaatgaattt atcaacaatc gatctttttg agatcaatga
     1021 agcatttgca gcttcttcta tcgtagttag ccaagaactg cagttagatg aagaaaaggt
     1081 caatatatat ggaggagcaa tcgcattagg ccatccaatt ggtgcaagcg gtgcacgtat
     1141 cttgactaca ttagcttacg gattgcttcg tgaacagaag agatacggta ttgcttcatt
     1201 atgtattggt ggaggtttag gcttggcagt cttgttagaa gcgaatatgg agcaaacaca
     1261 taaggatgtg caaaaaaaaa agttttatca gcttacgcca tctgaacggc gcagtcaatt
     1321 aatagaaaaa aatgtattga cacaagaaac tgcgttgatt tttcaggaac aaacactttc
     1381 tgaagagctc tccgatcaca tgatcgaaaa ccaagtgagt gaagtggaaa tcccaatggg
     1441 gatcgcgcag aattttcaaa tcaatggcaa gaaaaaatgg attccgatgg caacagagga
     1501 accatctgtc attgcagctg caagtaatgg tgcaaaaatc tgtggaaata tctgcgcaga
     1561 aacacctcag cggctgatgc gagggcaaat cgttctatca ggaaaatcgg agtatcaagc
     1621 agtgatcaat gcagttaatc atcgaaaaga agagctgatt ctttgtgcaa atgaaagtta
     1681 tccttcaatc gtgaaacgcg ggggcggtgt tcaagatatc tctactcggg aattcatggg
     1741 gagtttccat gcatacttat ctattgattt tttagtggat gtaaaagatg caatgggcgc
     1801 taacatgatc aactcgattc ttgagagtgt ggcaaacaag ttacgggagt ggttccctga
     1861 agaagagatt ctattcagta ttttaagtaa ttttgctaca gaatcattgg cttctgcctg
     1921 ttgtgaaatc ccattcgaac gcttagggag aaacaaagaa atcggtgaac aaattgccaa
     1981 aaaaatccaa caggcagggg aatatgcgaa gcttgaccct taccgtgcag caacgcacaa
     2041 caaagggatc atgaatggta tagaagctgt agtggcagct acaggaaatg atacacgtgc
     2101 ggttagcgct tcgatccacg cctacgcggc aagaaatgga ttataccaag gattaacaga
     2161 ctggcagatc aaaggggata aactagttgg aaaactaacg gtgcctttgg ctgttgccac
     2221 agtaggcgga gcttccaata tcctgccaaa agcaaaagca tctttagcta tgttagatat
     2281 tgattcagcc aaggaactag cacaagtaat tgctgcagta gggttagctc aaaacctggc
     2341 tgctttgcgt gcattagtta cagaaggaat ccaaaaaggc cacatgggac ttcaagctcg
     2401 ttcattagcg atctcaattg gggctattgg tgaggaaatc gagcaagttg cgaaaaaatt
     2461 acgtgaagca gaaaagatga accagcagac agctatacaa atattggaaa aaatccggga
     2521 aaaataaaaa agcaatatct ttagaagaat aaagcaagat ttacagttat tttatttact
     2581 gcagatcttg cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta
     2641 aggttaggca tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg
     2701 cctaacattg taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga
     2761 tgttagaagg aatttctttt tacttgctgt a
//
LOCUS       DAXWLG010000224         2781 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXWLG010000224 DAXWLG010000000
VERSION     DAXWLG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.231"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            2..979
                     /locus_tag="ACGE4T_002618"
     CDS             2..979
                     /locus_tag="ACGE4T_002618"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010770321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8387823.1"
                     /translation="MLASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDLRAKL
                     EEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAYASRF
                     SMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGDGKIK
                     YVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVKANVI
                     PISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKVVVSK
                     VLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            1012..1815
                     /locus_tag="ACGE4T_002619"
     CDS             1012..1815
                     /locus_tag="ACGE4T_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFF8387824.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            1836..2750
                     /locus_tag="ACGE4T_002620"
     CDS             1836..2750
                     /locus_tag="ACGE4T_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8387825.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
BASE COUNT          839 a          487 c          640 g          815 t
ORIGIN      
        1 tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt
       61 tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt
      121 gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt
      181 agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa
      241 agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc
      301 acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga
      361 cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga
      421 agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa
      481 atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac
      541 aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct
      601 gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat
      661 tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt
      721 atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt
      781 aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat
      841 gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt
      901 acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc
      961 tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt
     1021 atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat
     1081 gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac
     1141 ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat
     1201 atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta
     1261 gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg
     1321 gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat
     1381 ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt
     1441 gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca
     1501 gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct
     1561 atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca
     1621 aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca
     1681 ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa
     1741 atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt
     1801 ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat
     1861 caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact
     1921 acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat
     1981 acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac
     2041 acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga
     2101 atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt
     2161 tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag
     2221 gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga
     2281 acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta
     2341 aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt
     2401 tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag
     2461 ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag
     2521 gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg
     2581 aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta gctgcattag
     2641 gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc
     2701 ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa
     2761 tatagctcgg gcgttgtccc g
//
LOCUS       DAXWLG010000225         2736 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXWLG010000225 DAXWLG010000000
VERSION     DAXWLG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.232"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..533
                     /locus_tag="ACGE4T_002621"
     CDS             <1..533
                     /locus_tag="ACGE4T_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387826.1"
                     /translation="SFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILT
                     LAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSN
                     RRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFS
                     FAREDEDNPKTYDILA"
     gene            complement(594..959)
                     /locus_tag="ACGE4T_002622"
     CDS             complement(594..959)
                     /locus_tag="ACGE4T_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFF8387827.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1035..1280)
                     /locus_tag="ACGE4T_002623"
     CDS             complement(1035..1280)
                     /locus_tag="ACGE4T_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8387828.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1350..1604)
                     /locus_tag="ACGE4T_002624"
     CDS             complement(1350..1604)
                     /locus_tag="ACGE4T_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387829.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1768..2091)
                     /locus_tag="ACGE4T_002625"
     CDS             complement(1768..2091)
                     /locus_tag="ACGE4T_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387830.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2248..>2736
                     /locus_tag="ACGE4T_002626"
     CDS             2248..>2736
                     /locus_tag="ACGE4T_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFF8387831.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         1029 a          466 c          471 g          770 t
ORIGIN      
        1 taagcttttt taaagaatat ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa
       61 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
      121 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      181 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      241 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      301 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      361 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      421 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
      481 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
      541 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
      601 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
      661 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
      721 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
      781 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
      841 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
      901 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
      961 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
     1021 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1081 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1141 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1201 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1261 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
     1321 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
     1381 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
     1441 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
     1501 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
     1561 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
     1621 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
     1681 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
     1741 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
     1801 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
     1861 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
     1921 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
     1981 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
     2041 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
     2101 ataaaagaat aacatttttt ctaaaataag caaataaaaa gagagtataa aatattttgt
     2161 gtaaatgaaa aaatccatac aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa
     2221 ccaattcaca gaaaagagga cttccctatg aatgatttta ctacagaaat tgtgcaaact
     2281 ctagtcacta aaggcgattt aaatgaatta ttccgttcgc acttagaaaa agcgataaac
     2341 acactcctac ggactgaatt aacggctttt ttagattacg aaaaatatga tcgcactggt
     2401 tttaattcag gtaattcgag aaacggttct tactttcgat caatcaaaac cgaatatggt
     2461 gaattaacat tggaaatacc tagagatcgt aatggtgagt ttaaacaaca aactttacca
     2521 gcctacaaaa gaacaaacga tacattggaa accactatta tccatttatt cgaaaaaggt
     2581 gttacgatgt ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca
     2641 caaaccatgt ccaacatgac taaagttctg actgaagaag taaatgcctt taaatccaga
     2701 gccttaaatg ataagtatgt cgctattttt atggac
//
LOCUS       DAXWLG010000226         2642 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXWLG010000226 DAXWLG010000000
VERSION     DAXWLG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.233"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(195..773)
                     /locus_tag="ACGE4T_002627"
     CDS             complement(195..773)
                     /locus_tag="ACGE4T_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8387832.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(793..1461)
                     /locus_tag="ACGE4T_002628"
     CDS             complement(793..1461)
                     /locus_tag="ACGE4T_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387833.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1476..2546)
                     /locus_tag="ACGE4T_002629"
     CDS             complement(1476..2546)
                     /locus_tag="ACGE4T_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8387834.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT          777 a          572 c          396 g          897 t
ORIGIN      
        1 aattaatttc ttcgttatta aattaaaaca cttcctggaa caccgacata ataaataaaa
       61 atataccctg ataatcatga gagaataaca aatttgtatt tatgaaaaac tacctatata
      121 aaaaaaacaa gatataacga tcttcgtcat atcttgcttc ttcacctaac tatttttctc
      181 cctatataac agctttacgc ttctcctaat acaccattca aatataaatc gatcaattgc
      241 gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg tgctaacact
      301 aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac agattggttt
      361 gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa gaaaagatga
      421 tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa caaatcgacc
      481 agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg
      541 cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt ttctaaaact
      601 gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggttg ggtgatccca
      661 actttttgtg cgatctctct tgttgaagtg ccgagatatc ccttggacat aaacaagtca
      721 gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac catttttcca
      781 cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa atattctatc
      841 gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat ttttgtcttt
      901 tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca aactagcagt
      961 tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga ttgcgaccct
     1021 ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat ccacgccaag
     1081 ctgctcaaat aattcttttg cttctttttg acgatgttca tgtttgatct tatctactaa
     1141 taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca aaataaagcc
     1201 aatttcttta aaacgtagtt taacgcgttc actttctttt tgattactga attcttgatc
     1261 attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt
     1321 acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat cgatactcat
     1381 ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt tttctacttc
     1441 tttaaattct attgctttca tgctcttcct ccttcttatc caattgcctt caacggatcg
     1501 atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac cagcatcaac
     1561 aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc aggtagaaca
     1621 agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga taaaatgaat
     1681 gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc tttcattaca
     1741 ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat cacaatagcc
     1801 gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc attatatcca
     1861 ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg taaattactg
     1921 attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg
     1981 aagcggatct cttggtaagc attgatggtt gtatagagaa ctggtgagac attgaatttg
     2041 gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa cttaactgta
     2101 tctcctacgg ctagaccata ttcttctttc aaactaatat cgcctactgc ctcatcatcg
     2161 ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt atcgataccg
     2221 aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac gactccaggt
     2281 gtctgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt cttagtaatc
     2341 atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc tgcctcccac
     2401 ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt caaaaaaaat
     2461 accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa ctttgaatgc
     2521 ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc attttataca
     2581 acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt cttccgctat
     2641 tt
//
LOCUS       DAXWLG010000227         2619 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXWLG010000227 DAXWLG010000000
VERSION     DAXWLG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.234"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(65..1108)
                     /locus_tag="ACGE4T_002630"
     CDS             complement(65..1108)
                     /locus_tag="ACGE4T_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFF8387835.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1485..2450)
                     /locus_tag="ACGE4T_002631"
     CDS             complement(1485..2450)
                     /locus_tag="ACGE4T_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFF8387836.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT          932 a          482 c          496 g          709 t
ORIGIN      
        1 taaaaacagt tgtgattcgg acattgttcc atgcgagaat cacaactgtt ttttcatgtt
       61 cattctaaac tttgatcagc caaataaata gtaaagccat cactgtttgg agaagcccga
      121 cagctaatcc ataagataag aagcgtgtac cttctttcat gaattttgca aagtctaaac
      181 gcagaccaat tgccgctaaa gcagtgatct caaaccagct gctgataaaa tgtgccgatt
      241 ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa acaaaagatc acaaacccta
      301 atacatacca aggaagcggt aattttcgtt ttttctgcgt atcagttggc gtttcaaaca
      361 gagctggttg tttcttgtgc atgaattttt caaacagata gacaacaaca acgagcatca
      421 taatacgtag aatcttaaat agcatggcga attcgacaac agaattgtca agtaagcttg
      481 ccccagcgac tacttgacca acagattgga gtgtaccacc caaaagtgca cttttttcaa
      541 tgagatttcc accaaacaac tgtaatccaa gaaaagggag agtcaacatc atcacagtac
      601 ccagtagatt gactagtgtg atgatctgtc ctttttcttc ctctttcgca tggatggaag
      661 gggcgattga agcaatagca gaagaaccac aaacagcgtt tcctccagcc atcatcaaag
      721 aagaagtatc gctgaattgc agttttttac caatcaagta agcaccgatg atagtgactg
      781 acatttgcag taacacaaaa acaattcctt ttacaccaat ttgagcaatc gtttgagctg
      841 tgacagttgc acctagtaaa aaaacagaga attctaatag tttgctttca gcaaattttg
      901 tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc taataaaata cccaataaaa
      961 tagcaatagt cgctccacct aactgtggca gataaatagc aagaagcctg ctgaaggcag
     1021 cgatggcaaa agaaagcagt aaaccgggta agatttcgcg gaccgctgaa tcctttacag
     1081 attttattgg atgtgacaat gatttcattt gatcggttcc tttcgtcaat aaatactaaa
     1141 agcatactcg aacatagtag cactaaacca gtattttgac aaaagaacaa gaggtcgtga
     1201 caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt tccagaagta gcttctacgg
     1261 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
     1321 ttgaagctga ccacttcttt tacgacctct tggtaaacgg tgttcaaaag cttgcatctg
     1381 cggtaataag cccaaccagt acaaaaacat gaacagctgt ttttgtttgg ccgttcttat
     1441 tacttgatgc agaacagctt tttcacaacc tattgaatcg attattaagc gattttttta
     1501 gaaacaaatt ttccatgttt gctatgttct ttaattaaga aacgtacgta atgagcaaga
     1561 acgtagaaga caactacact tgcaaacaga cattccgcaa cttttaagaa atccaatacg
     1621 ctactataga tacctaaact gttgaagtaa tgaacagtag taaatatagc tgttgcagag
     1681 ataacaagcg ggaatgtaaa ggctgcatag cttggataaa aacgaagctt gaacaaacgt
     1741 ggaagcatag acagcacaac gaaatacaat acttgagata aagcgaacaa tgctacgaca
     1801 agataaaaat taggttcagc aaagttttta agataacctg ttaggcaaag agaaccaggt
     1861 gctgcaacga tcgtaatcag cggacgtgtc ggatgagcca tatttcttac gaaaaagaca
     1921 cgtttgatga tgatcggcaa taagaacgca tagcagatca atgaaatcca aaagaaaatt
     1981 tttccaataa gtgggaagaa gttcccggca gttacagtga tgaccccaaa tccaacgtaa
     2041 atgataaacc agcttggata aattgcttca attgaaagat cagctttgat aacatggtga
     2101 taattgaaat aaaagacaag gatcacttgg aagataacag cgcacagcca aatgaatttg
     2161 acaattggtg caatactttc atatgaaaca aaataagtac aaataaccat gagtgacatc
     2221 gtaaaagttg gtgaaacgga ggcgatgatt ggatcatgca acgtttgttt cgtgtgttcg
     2281 aataagataa acaatttaac aaaaatgaat atcataagaa tcatagagat accgccagtt
     2341 agattgccta aagcaacatg gtgatagtct ttaaacagat ttccaaggga agctaaccca
     2401 aggatcaacc cgcagatcgg gatcggaata attttcaaaa agttcttcat atataatatt
     2461 ctccttttta tttaatagat tttagtacca agatgaacga atacattgta aaatgaatag
     2521 gaatgtatga ttacgagcaa gcagacagct gattgtcttc atctaagtta ctagttggat
     2581 cagcaactgt tttgttgtac atttctatta tagatggtt
//
LOCUS       DAXWLG010000228         2571 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXWLG010000228 DAXWLG010000000
VERSION     DAXWLG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.235"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(69..971)
                     /locus_tag="ACGE4T_002632"
     CDS             complement(69..971)
                     /locus_tag="ACGE4T_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8387837.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(968..1660)
                     /locus_tag="ACGE4T_002633"
     CDS             complement(968..1660)
                     /locus_tag="ACGE4T_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFF8387838.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(1703..2344)
                     /locus_tag="ACGE4T_002634"
     CDS             complement(1703..2344)
                     /locus_tag="ACGE4T_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFF8387839.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(2382..>2571)
                     /locus_tag="ACGE4T_002635"
     CDS             complement(2382..>2571)
                     /locus_tag="ACGE4T_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387840.1"
                     /translation="NFYRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGA
                     VSKMVKSGNVANISYLMI"
BASE COUNT          711 a          573 c          423 g          864 t
ORIGIN      
        1 aaaattctgt cacaacccct cttttttgat caacggtgtt caataaacag cgttctcatc
       61 tgcttttctt agctctgctg gtccagtaac cgagtaagct ctttgataaa tctttgagcg
      121 atttcactga gcggacgttt cgccgaataa atgatataaa attcatttag ttcgccttct
      181 gccaaaggga ttaaccgcaa gtgctcgcga atccctccaa tcacaatacc agatcctgaa
      241 gcgtaagcat cagtttcaag aacgatattc atcaaagtac ctcgatcact cgtataaatc
      301 actgtttctt gatcagggat ctcgataagg tcttcataaa aatacgggaa attatttcca
      361 tcctgtgtaa aacgaacttg aggataagat aacaattctt ctttggtgat ctctgcgtgt
      421 tcagctaatg gatgtccttg ccttaaaaaa atatgcgttt tgaattttcc aatgctgcgg
      481 ccacttagct ctccttgttg taaatatctt ctgataccag cttgattatg ctcattcata
      541 taaataatgc cgatctcact gtgctgctct ttgacttctt cgatcactct cgctgttgtc
      601 gtctccatga tccgaaattg tttgatctcg tctttgaatt cctcgagtag ctgtgcggtc
      661 aaaggaccta agaaatcata atgctgggca gcgatcgaaa aattgtctct cttttcactc
      721 gttagatagt gagtttctaa tgtatcaagc tggaccaata ttttctcggc acgatttaag
      781 aagtcttgcc cttcttctgt taaatatgcc cctcgattcg ttcgagtaaa cagcgtgatc
      841 cctaactcgg tttctaattc tttgatacca gctgaaagac ttggctggga gacaaacatt
      901 ttctttgccg cttctctgaa actaccattg tttgctacag cgaccacgta tttcatctgc
      961 tgaatattca ttgctcaaac tcctcgaaat aataaatttc tcttccattc aagggctgga
     1021 ttggtcggtt gttcggtcga gcatactgac catcgaaggg gagaagaaac tcttggttca
     1081 gttttcctac atgatccatt acaaaccatt ggattggccc gctggtaggc ggagttgtca
     1141 atgagccaat ataatggaaa tggctttttt gctccggaaa gaacacatcc ggatttaccc
     1201 aatgctcatg tttttctaag ttccaacttt ccccattttc ttgattatac agccaacctt
     1261 cctgtaccat tagctggtac atgacaccca caactagatt ctcttttttg ctgttcatat
     1321 gaacaaaatg aaattcaata tcttcctgtt ttccgttgat gatatgttca gaaggcaagt
     1381 ggaaatgaat atctgtcaaa taatagcgtt ctttcttgaa ctctacataa ctgagttggt
     1441 caaaaggtac taaatgaatc gtgtttttga attccttgtc agtaaaccgt tgcttttgat
     1501 aatgaaagga aagcgtgtct ccttctattt tggtttcttc ccccttggtc aaatgaatag
     1561 gcgactgcaa tggaaattcc gcacctctcg caaaccaatc acataaactc gcccaatttt
     1621 ccggaccttt gtctccagaa tagccccatt cgacatccat attctttttc aactatactt
     1681 ccccctgcat ctcatatcat ttttattttt cttctttctt tacaatcgtt gagacggttt
     1741 ctaaaaacgc ctcttttcca taaaattcct ggttcaatag ttccggaaat atctctttaa
     1801 agaaatactg aacagaaggc cgatgttcaa agcttttaat cgtagtcact gcctcaatga
     1861 acatctcctt ataaactttt tccgggttat ttttgatgat ttcatcaaaa gattcataaa
     1921 gaaggtcaac cttatctgaa atggctaata gttctccttc tacggtttca tcttttcctt
     1981 cgaacaaccg acggcgataa acttcttgga actcagctgg aatctcttgg ttgatgaatt
     2041 cgtccgtcat cttttcttca actgtttgca gcatccctct taactctttg cttgcgtatt
     2101 tcaccggcgt tttgatatca ccaataaaac gctctgtgta gtcatgattc aatgattttt
     2161 catagagcag tttccaatcg atcggttgtc cattcgcttc ttcaatgtct cctagaactt
     2221 gcgcaataga tgctacacga tacgaatgtg ctgcaactgt atgctctgta tatttaaaaa
     2281 atccaggtgc ccttgtgatc gtctctaaat tgttcaatcc taaaataaat tcattcaacc
     2341 ccataaaagt tacctccctt tgagatttct ttttccgaat atcatatcat taaataactg
     2401 atattcgcaa catttccgct ctttaccatt tttgaaacag ctcccttacc aaaaaataga
     2461 gaaaatctat atataataga aaaaagagaa gagattattc tcttctttct gctaaccagt
     2521 cttagaaaaa tgaattttaa aaaaatcaaa aactgatcac gatagaagtt t
//
LOCUS       DAXWLG010000229         2550 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXWLG010000229 DAXWLG010000000
VERSION     DAXWLG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.237"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..493
                     /locus_tag="ACGE4T_002636"
     CDS             <1..493
                     /locus_tag="ACGE4T_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8387841.1"
                     /translation="VIPPAIFFYYSTLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQ
                     DLFELIDNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREG
                     IELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSE
                     VNN"
     gene            707..1402
                     /gene="vanR-A"
                     /locus_tag="ACGE4T_002637"
     CDS             707..1402
                     /gene="vanR-A"
                     /locus_tag="ACGE4T_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8387842.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1380..2534
                     /locus_tag="ACGE4T_002638"
     CDS             1380..2534
                     /locus_tag="ACGE4T_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8387843.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          873 a          460 c          528 g          689 t
ORIGIN      
        1 cgtcattcct cctgctatct ttttctatta ttctacctta tttgatagta gatttaaaac
       61 tttgcaacag aacctacagg aagatgacat catttatgtt acagacttaa ctcgaatcac
      121 tcgtagtaca caagatctat ttgaattaat cgataacata cgagataaaa aggcaagttt
      181 aaaatcacta aaagatacat ggcttgattt atcagaagat aatccataca gccaattctt
      241 aattactgta atggctggtg ttaaccaatt agagcgagat cttattcgga tgagacaacg
      301 tgaagggatt gaattggcta agaaagaagg aaagtttaaa ggtcgattaa agaagtatca
      361 taaaaatcac gcaggaatga attatgcggt aaagctatat aaagaaggaa atatgactgt
      421 aaatcaaatt tgtgaaatta ctaatgtatc tagggcttca ttatacagga aattatcaga
      481 agtgaataat tagccattct gtattccgct aatgggcaat atttttaaag aagaaaagga
      541 aactataaaa tattaacagc ctcctagcga tgccgaaaag ccctttgata aaaaaagaat
      601 catcatctta agaaattctt agtcatttat tatgtaaatg cttataaatt cggccctata
      661 atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga gcgataaaat
      721 acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact taaaaaacga
      781 gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta tagacaagtc
      841 tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc ttactatctg
      901 tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga aagatacaga
      961 ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga agccctttcg
     1021 cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa aattcagtgg
     1081 agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta atgttaacac
     1141 ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt tttcaatact
     1201 gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat ttcatgagat
     1261 atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata tccggcattt
     1321 gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg tatggggggt
     1381 tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga aaactttaca
     1441 tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt cgttcaatga
     1501 tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat gacttaaatc
     1561 acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat atctttattt
     1621 atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca aaattcgcaa
     1681 aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa gataaacaaa
     1741 ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta aaacggactc
     1801 tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac gttgttatgt
     1861 acttggcgca cgatattaaa acgcccctta catccattat cggttatttg agcctgcttg
     1921 acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc acgttggaca
     1981 aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg tataacctac
     2041 aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg cagatgaccg
     2101 atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac gcccccgagg
     2161 atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac attttgaaaa
     2221 acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc ctctccgggg
     2281 atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag ctagctgcca
     2341 tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt ggcgcgggac
     2401 ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt tacgcggaaa
     2461 gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac ttggttgata
     2521 aaaggaggtc ctaagagatg tatataattt
//
LOCUS       DAXWLG010000230         2540 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXWLG010000230 DAXWLG010000000
VERSION     DAXWLG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.238"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(31..459)
                     /locus_tag="ACGE4T_002639"
     CDS             complement(31..459)
                     /locus_tag="ACGE4T_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387844.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(487..1125)
                     /locus_tag="ACGE4T_002640"
     CDS             complement(487..1125)
                     /locus_tag="ACGE4T_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8387845.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1129..>2540)
                     /locus_tag="ACGE4T_002641"
     CDS             complement(1129..>2540)
                     /locus_tag="ACGE4T_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFF8387846.1"
                     /translation="LLLSSFFLLMNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIA
                     GIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVEKIASLREAFNYIQYILF
                     TFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFI
                     YSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVS
                     GRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYT
                     FIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIKIGSLSKERLFELIEESQ
                     LRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRK
                     IAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILS
                     VVFILSTEKKSQLLLMNGG"
BASE COUNT          834 a          475 c          375 g          856 t
ORIGIN      
        1 tttttctatt ttatataaac ttttggatac ttaataccaa ccggttggat tgtaccaaca
       61 tgagaatgtg tcagttctat ttcctgtcgc acttgcatag gcccatactc cttttgttgc
      121 atttgctgat cgaatagagt ttccaacttt cgcttttgca ccgtgacgat tatagacacg
      181 actatagtgg ttagaatgac ctgtttttct cgtacaagtt gtatcactat aataaaccca
      241 tcttccagag ctatgattaa ttgctgttct agaaatttcc cggtctactg gtaaatttga
      301 cttaataata ttacctttag catcaaaatg aatctctcct ggctttaaag atttgtaata
      361 gatttcaccg tgaactgaat ttgcttccac ttcagatgac atagcgtttc ctccaacaaa
      421 acctatccct agtgcacaaa taataactgc ttttttcata aaacgacatc ctttcctttt
      481 tgatttttaa agcatgatct tcttgtttcc tattttggtc agatatggat catgcgtgac
      541 tacgataact aacgtacctt gggaagcttg ttgcgataag atttcaacta ctttatcctt
      601 atttctttta tccaatgacc cagtcggttc atcggctaaa atgattttgc tattcttcaa
      661 taagactctc gcacaggcga ttctttgctt ctcaccacca gataatgtgt agataggcgt
      721 acttagcttc ttgtttatat ttactttttt caatgcctca tactttgctg ttcgcttatc
      781 tgaaacattt aaattcgaaa acttcaatcc aatatctaaa ttaaaatcga ttgtttcata
      841 ttcaatcaac ccaaaatctt gaaaaagaaa agatatggta tttcttttta tcaagcgctt
      901 ctctctccca gttaattgtt tcgtctcttg atcgaacaaa tagaattgtc ctgaactgaa
      961 ctgatcattc aatcctaata tattcaataa cgtactttta cctgctccac tttctccaac
     1021 aacagtcaca agatccccag catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt
     1081 tcgaatgatt tttttcgcac cttttatcac tatcaacggc tccaattgtt aacctccatt
     1141 catcagcaat aactgacttt tcttttctgt agacaaaata aaaacaactg aaagaataaa
     1201 atcaatgaag ctaaagaaaa aaatattaaa aagatattta ggctctcttt ctataagaaa
     1261 aaggaagatt acacttgtcg ttccccattt gatgaggttc atcatgaaga aatctttata
     1321 cgtttcaaag agcgaataac cacttgaacg aaagatagcg attttccgac aattgtagac
     1381 aaaatatatg cataacatga catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa
     1441 tatctgtatc aagtaatgaa atgatctgct taacactttg acagctatct cttcagtctg
     1501 agtatacgga aatgataact gcaagttatc acgtaactga ctttcttcaa tcagctcaaa
     1561 taacctttct ttcgacaaag agcctatctt gatctttaac ggtggataga tttctccgct
     1621 gagaatattt ctttccaaat acatactgtt tttcaatgtc agtatttcaa caaccggata
     1681 atgttcgatc caggggatag aaggaataaa tgtataaatg ggttgattgt tttttataaa
     1741 tagaaatttc ggttcttttt cactgatttc ttgataatat tctgttattt gcgccaggtc
     1801 attttttaaa tctgtatgtc tatatcgttc agggatcaat ataacagggt gcgtttcttt
     1861 ttcggttatt tggatcaacc ttccagagac atcaatcaaa ggaaatttct taagataatt
     1921 cagatttatt tttatccctc tgccaaactg ttcattttct tcattcaagt aatctgatac
     1981 attgactaat atacttccgt ttttattcaa agtttcatat aattcctgat ctgctgctga
     2041 tgcaaaacga ttggaataaa taaaatccac gtggtttttc ccgatggtta ctggataaaa
     2101 tacatgatag tcctcaccag cgatttcttt tggtggttca tgggaaacag ctaactgtga
     2161 agaagaaaaa acgatagttg ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa
     2221 caatccaaat gtaaaaagaa tgtattggat atagttgaac gcttctctta acgaagcgat
     2281 tttttcaact aataaaagag aaatcaaaga gataccatat attccgctga acaagaaaac
     2341 gtacatctgg aacaaataaa gaaattgctc cttataatac ataaaaataa aaccaccaat
     2401 gattcctgca attatgaatg taataatgat tttctttttc cccaataaac accacaatac
     2461 ttgatgtaat gaaaagtcat tattcaacaa taaactgatt tctttattca tcaataagaa
     2521 aaaggatgat aacaaaagga
//
LOCUS       DAXWLG010000231         2508 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXWLG010000231 DAXWLG010000000
VERSION     DAXWLG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.239"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(12..1052)
                     /locus_tag="ACGE4T_002642"
     CDS             complement(12..1052)
                     /locus_tag="ACGE4T_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8387847.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1401..1727)
                     /locus_tag="ACGE4T_002643"
     CDS             complement(1401..1727)
                     /locus_tag="ACGE4T_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387848.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1714..2502)
                     /locus_tag="ACGE4T_002644"
     CDS             complement(1714..2502)
                     /locus_tag="ACGE4T_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8387849.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          708 a          437 c          356 g         1007 t
ORIGIN      
        1 ctttttatca actactctag ccaattttcc gtactaactg ttggtaattc gttaaatgtt
       61 tctctttgtt cgtttgcaag ctttgccgca atagcttttt cgacaaacca attttttaca
      121 taaacgaaaa ttagattctg caaattgttt actttttcag ttttttgttt ttgataagct
      181 ttcagcaaag tcatatataa tcctttgtca gcatcaattc cataagattc cagttcttca
      241 taaacaacaa ctgtttgctc taattcttca gcttttttct ttgcattatg aatagtttgg
      301 acggtttttt gagcgtcttc gtaagtacta gaaaatgttt ttatcaagtt taatacattt
      361 tctggtacaa atgtatttat actgtcctct ttcatgatgt cgacgaagtt atctagtaaa
      421 acttgatttt gtaactgatc agtttcagtg tctattatgt gtctattagt gtctatagaa
      481 ttagaataga atagattgtc tgcgattttc gcaattacgg ttgagtcctt gatatgactg
      541 gattttgccg tatttgcgat tttcgcaatt acggtggtat cattgatttc tcgcggtttt
      601 gccgtatttg cgattttcgc aattacggtg gtatcagtat tgtcttcctc ttccatgatt
      661 ttaaagacgt cttgattcgt tacttctggc ttgcctaagt acaataaatt aggcatggat
      721 tcaatcttac cgtcacgttt atttactctt cctctcttct gtttcagaag acctgctttt
      781 tctaattcct ttttgatttt agatacttta ccttcgcggc attgtaacaa tttcattaat
      841 tcttcaactg tgaatatgaa aaatatatta tcatcagaat ctacccagtt atttttgact
      901 gaataatcaa atcgatcttt aagtagcata taagcaattt ttgcatcatt tgatagatcc
      961 atgtattttt cgttagtaaa aaatacctta ggcatttgat aatatttttc acgataaatt
     1021 tcttttgatt tgtaataatt aaaatcattc atagccaatc ctctttctgt aggatagcgt
     1081 tttgttcaaa actatgttac aatgaattaa acaaatatat ctatccacta tatatttttc
     1141 tactgctgat aagtcgccaa actttgttaa agcagtagtc tttttttatc tttttttagt
     1201 ttaccttaaa aaataaatta aagcaaaccg ctctatttca aggtttaagc tcatagtaac
     1261 tatatcacga tattacaata atattatatc acgatatcgt aatattacaa tatgaagata
     1321 agcgacttat cagctttgat actactgatt tttataaaaa aaatatcacg atattatgat
     1381 atcgtgatat tgaatgtaaa ttaaatttta ttttgtttct ttgctagttt atatccttca
     1441 atcaagtatt cttcaggtat ggactttaat agcgcatctt ctataacgtc tggtttactt
     1501 ttgtctgatt cagcaatagt cgtccaatat ttgagtgctg acattagaat tggatttata
     1561 cgaatgctat ctgctgttct tacactactt ccttgatctt ttatgacttt acttatattt
     1621 ttcttggtta attcgatttc ttctacttct tttgatttaa aattcttttt atttttattt
     1681 cctagtaatc tacttactgc agcttgactg cgattatctt ctgccatcaa aatcaccaac
     1741 ttcttttaaa tattcttctg taaatgcttt atagtcatct tcagctgttg agggtttatt
     1801 aaaattgatt agatctgatc cagcagcttg cgcttcgtca acggttattg tcctacgaat
     1861 ataagttttg aaaatttttg caccaatgac tctagaaatt tcggttgctg cagacgtcat
     1921 ttctttagaa atgttataac gcttattata cttagtaaat agaactcctc gtacttcaag
     1981 cttgtcgttc gtatattctt ttaccgtttc aatagtttca cttaacttta ttagtccttt
     2041 taatgagaaa tacgcagctt caactggaat gataatttca tcagaaacag taaatgcgtt
     2101 gactgtaagc attcctaaac ttggtggaca atcaattata atataatcat agttgtcctt
     2161 gattggttga agtactcttt taagcctgtg ttcacttcct acttgagtaa attccctctc
     2221 tgcagaagaa agggctatgt cggctggcaa aacatcaatt cctttatagt ccagaatagc
     2281 atctgtaatg tcagcctccc cttttaatac ttcataaatt gtaggttggt ttgttccatc
     2341 tatatcgaaa cctacattat ctgtagcatt tccctgagga tcaatgtcca ctaaaagtac
     2401 atttttaccg taaacaaatt tcaaacatgc tgctaaatta acgctagtcg tagttttaga
     2461 aactccacct ttttgattgg ctactgacaa tattcttgcc attaaaat
//
LOCUS       DAXWLG010000232         2476 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXWLG010000232 DAXWLG010000000
VERSION     DAXWLG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.240"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            134..775
                     /locus_tag="ACGE4T_002645"
     CDS             134..775
                     /locus_tag="ACGE4T_002645"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8387850.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVPPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(837..2468)
                     /locus_tag="ACGE4T_002646"
     CDS             complement(837..2468)
                     /locus_tag="ACGE4T_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFF8387851.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT          798 a          550 c          450 g          678 t
ORIGIN      
        1 taaatttaaa atcaaaaagt aaaacaaaaa ttcaagcaat acttctgctt ttctatcctc
       61 acagttctac caaataaaat agtcgcattg tgtttctttt attcttataa taaaagaaaa
      121 aggaggagca taaatggaat acccaaaaga actgatacaa gaagcttctt taagaatgaa
      181 cggccgtcct gtagaaggat ttatcgctgg gcaaggacct cttcatccta aactgatgct
      241 cgtgggagaa gccccaggga aaacagagat tgatacgcat gttcctttta gcggtcaagc
      301 tggaaaagaa ttgatgcagg cactttcttc aactggctta acacgagaag aggtgtatat
      361 caccagtgct gtaaggagcc gcccgtatcg cgtgactcat cggatcaata aacggacaca
      421 acagtcagaa actgtttatc caaatcggac acctactcgt tccgaagtat ttgcgcatgc
      481 accaatattg gattacgaac tgatgaaggt cccgccaaaa ttaattgcga cacttggaaa
      541 cattggtctt caacgtttac ttggcaaaga atatacggtt accaaaaatc atggtcaact
      601 ctatacaggg cctgtcttac aattaacaga acaaaaggat gcctacgaat ggtcagaaaa
      661 aaactatcga attcttccac tttttcatcc agcagctatt ttctacaatc gctcccttgt
      721 acctgttatc catcaagact ggcaagtact agcttcactt ttacagcaat attaaaaaga
      781 acagacaaga ccataaaatc ttcagtttac agtcttgtct gttctttttc attgttttaa
      841 tggctaattg cagtatattt cgcatgtaaa gctgtcaacg catgagcaac tgtttgatgt
      901 tgatgcgtga atgtatgttc ccacaagtga gaaagttgtt tttctacttc tggctgcaag
      961 gaagtataga taatcgtttg tcctctttgc tctgcttttt cttgaaattc atcaaaaact
     1021 gcacgtgccg tcaaatcaat acttggtacg cctcgcatgg agaaaatgac accatcgtga
     1081 tgatttagtt cttccaattt atttttgagt ttttctgaag acataaaaaa cagtggacca
     1141 ctaatataca ctactgtcca gttattcagc ttttccgttt ctggcagccc cattctttgc
     1201 caatcgatcg tttctaatga aatcgtgata gcagcgcttt tgacaacaaa aaatacacag
     1261 ccgccgatca ccccgatcac gatagcaata cttaaatcaa agataactgt acaaatcatc
     1321 gtaaggataa acaatagaac tgctgaccaa tatttattag taaatagctc cttgatcgtt
     1381 tcccattcat tcatacgcca agccgtcaca atcaaaacac ctgcaagtgc tggcattgga
     1441 atctgtgaca tcactggcgc aaaaacaagc atggagagca gtaagacaac tgcatgtatc
     1501 ataccagcta atctagtttg tgcaccagat ttgatggcca cgcttgtacg ggcgatggcc
     1561 gcagtagcag gaatcccgcc aaagaaagga agcaacagat ttccaattcc ttgcgccatt
     1621 aattcttgat tgctatctaa gggcttattt gccattcggc cagccgaagc accacatagc
     1681 aagctttcaa tcattcctaa taaagcaatg ctaatagctg gaactgccac ttgttgcaag
     1741 gcagaaaagc taaaatctcc catattcaaa cgattactac tgatcaatgt ttgtggaatc
     1801 ttacccacag ttgctactgg taaatggaac aatagcataa ctgctgttgc caaaatgata
     1861 gccagcagcg aactaggcat tcgtttcgcc catttttttg gatagataaa catccctaaa
     1921 atgactagcc ctcccatgat caaagaggct gtgtttatcg gaaatcctat cgtttgataa
     1981 ctacttagtt tttcaataac gtttgtacct tccgagtgaa caccaaataa attatcgatc
     2041 tgacctaatg caataatgat agcaatacct gaagtaaacc ccgtaattac gggtgctggg
     2101 ataaaagaag tcagtgttcc taatcggaaa agtcctgctg caagtaaaaa tagacccgct
     2161 aaaaaagtag caagcaatac accttgcatt ccctttgttg ccgcaataga catcaagata
     2221 gctgccatcg cacctgtcgg acctgatatt tgataaaaac cgcctgataa accgccaata
     2281 acgataccgg caatgatagc cgtaatcaaa cctgcggccg catcagctcc gcttgatacc
     2341 ccaaaagcca aggctaaagg caatgctaca gcagctactg tgatcccagc caaaagatct
     2401 ttctgaaatc ttgaaagatc ataacctgaa aattcttttt ttagtgagga aacataattt
     2461 ttgaacacct atctaa
//
LOCUS       DAXWLG010000233         2413 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXWLG010000233 DAXWLG010000000
VERSION     DAXWLG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.241"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            151..384
                     /locus_tag="ACGE4T_002647"
     CDS             151..384
                     /locus_tag="ACGE4T_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFF8387852.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            381..788
                     /locus_tag="ACGE4T_002648"
     CDS             381..788
                     /locus_tag="ACGE4T_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFF8387853.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(1925..>2413)
                     /locus_tag="ACGE4T_002649"
     CDS             complement(1925..>2413)
                     /locus_tag="ACGE4T_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFF8387854.1"
                     /translation="KKEINRTYTKYILEFETIPEELKDKRVEEVDRTPAENLAYQVGW
                     TNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKELEEILNRNIDD
                     INMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWKRL
                     AL"
BASE COUNT          817 a          408 c          372 g          816 t
ORIGIN      
        1 aagggagcaa tctaaatttt ttgaattttc ttaattaatt atatagaaag aacaatcttt
       61 ctatagagtt agacatgaga gggactatgc ttgatttaaa atcaacaagc gtatatactg
      121 tatatacaaa gaaaaaggag ggagaagact atggcaatta aagaaagaaa atttagggaa
      181 gtggggaact cagtagtatt aactatttca aaggagtttc ttaaagaaaa tgggttaaat
      241 cccggagata cggttttttt agatgaagaa aaacttcgag cggcaattac aaaaaaacaa
      301 gccttatctg atgaaattga tttagcaata tctcaagcat taactgaata tgatactact
      361 tttagagagc ttgtcgataa atgagtattc attacctaga agccaaagat attataaaaa
      421 tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc ccttggtgta aaggatgcga
      481 atgctttaga aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa gaggtttatc
      541 cttcgattta tgaaaaagca gctatcctac ttatacaatt gataaagaaa catccttttc
      601 acaatgcaaa taaacgtact gctttcttag ctacttttgt ctttttgaaa atcaatggac
      661 agcttttagc tattgatcaa aaagatgctg taaatttagc ggtatatatt gctacgtatg
      721 ataaggattt cgatcaacta aaatatgaag tgatcaacac tataaagaca catacgcagc
      781 ccatttaagg agttactgta tgtgtcttta tagggttatt agctgctata gcgaaataat
      841 tgaagtaaat tacaaatagc tataattcaa atccaactat atagaaaacg taaattttct
      901 atatagttgg attaagataa cgactattta ttttatcata tttttactat accctatact
      961 tttacttggt atatttatga ataatcgcaa atttacccct atactactag tttgtaagtt
     1021 agattaataa aaatactaaa gttatttgtt cttttattaa atacacctac aacttataaa
     1081 ttgatggtat ttagataaca cgaaagaata aaaataacag ctgatagtaa agtttgttga
     1141 cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga aaacttatga
     1201 aatgaaagac cttttacatc ttgcagaaat cgttactggg attaaactaa gcaataaaaa
     1261 ctaatgttcc cctcattctt tgaacttcta ctatttgatt tatttattct gataaatgac
     1321 gatttcacct tcaggatctt caaatcccgt atagaattta tttaagatct cttcagtatc
     1381 agtaatagga taggcgatag gccacatacc ctttgatggt tagaataaac tgctcaaaat
     1441 ctgtatcgta tgttggcttt agcatattaa caaaggctat ctccaaatta taaaccataa
     1501 gcgttgaatc gttcttaaca atttcaattc ttcctaaccc aaagtcagaa gatataatta
     1561 ccggacttgc cttacccttc atgtgggttg ttgagattcc atatttaaag ggaattgtcg
     1621 gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa atctttcccc tgctagtctc
     1681 ctaagtccct ttgtagcgga tatggcacca taatctcatt ttatcgaagt gagtcatggt
     1741 ttatctgttg gccaaccaaa attgatacaa tctaacgatg caccctctac ggcattctag
     1801 gggagtgcac ttgcattcac ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg
     1861 tatgttctta ctgttaacat acaagaggtc tcctttccac aattgcttag cagtaaacgg
     1921 gaatttacaa agctaatctc ttccattttc ttatctttgt tctaaaagtc ccaaatggag
     1981 caactgtatt tacatgaata aatttatata cttcccatac agccgttttg gttgcctcgt
     2041 ctgcccattt tcttttgtgt gcagtgaata gatcttcatc actcattgaa tcaatcatca
     2101 tgttaatatc atcaatatta cgattgagaa tttcctctaa ttctttcaat ggtagatgtg
     2161 cataggtatt tgtaaaccac ttatataact ctcctaactg attccattta aattgatcag
     2221 aaggcgtttt aacagataat ccatttcttt catcattttc ccattttaga attaaattcg
     2281 tccaaccaac ttgatatgct aaattttcag caggtgttct atccacttcc tcaactcttt
     2341 tatccttcaa ttcttcagga atagtttcaa attctaaaat atatttagta taagtcctat
     2401 ttatttcttt ttt
//
LOCUS       DAXWLG010000234         2405 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXWLG010000234 DAXWLG010000000
VERSION     DAXWLG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.242"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            28..240
                     /locus_tag="ACGE4T_002650"
     CDS             28..240
                     /locus_tag="ACGE4T_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387855.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(518..2317)
                     /locus_tag="ACGE4T_002651"
     CDS             complement(518..2317)
                     /locus_tag="ACGE4T_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFF8387856.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          841 a          440 c          450 g          674 t
ORIGIN      
        1 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
       61 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
      121 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
      181 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
      241 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
      301 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
      361 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
      421 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
      481 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
      541 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
      601 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
      661 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
      721 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
      781 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
      841 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
      901 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
      961 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
     1021 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
     1081 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
     1141 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
     1201 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
     1261 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
     1321 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
     1381 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
     1441 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
     1501 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
     1561 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
     1621 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
     1681 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
     1741 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
     1801 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
     1861 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
     1921 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
     1981 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
     2041 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
     2101 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
     2161 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
     2221 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
     2281 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
     2341 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
     2401 ttaac
//
LOCUS       DAXWLG010000235         2399 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXWLG010000235 DAXWLG010000000
VERSION     DAXWLG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.243"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..476
                     /locus_tag="ACGE4T_002652"
     CDS             <1..476
                     /locus_tag="ACGE4T_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8387857.1"
                     /translation="PQIQQLTNIVSPAVRGVFLKFLEKAESEKIPIQVVIPVEVKTIP
                     IDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRFSIANKANPTNLTLSEMV
                     KRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVEMYLASRKSKSAY"
     gene            798..1025
                     /locus_tag="ACGE4T_002653"
     CDS             798..1025
                     /locus_tag="ACGE4T_002653"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8387858.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            1148..1474
                     /locus_tag="ACGE4T_002654"
     CDS             1148..1474
                     /locus_tag="ACGE4T_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8387859.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1542..2255
                     /locus_tag="ACGE4T_002655"
     CDS             1542..2255
                     /locus_tag="ACGE4T_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFF8387860.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          901 a          383 c          499 g          616 t
ORIGIN      
        1 aaccgcaaat acaacagcta acaaatattg tatccccagc tgtcaggggt gtctttttaa
       61 aatttctaga aaaagcagaa tcggagaaaa tcccgatcca agtagttatt cctgtcgaag
      121 tgaaaacaat cccgattgat ctggttgttt ttattcgctg tttatctatt tttatttcta
      181 acgcattcga tcatcgtgta gccgatcaga gtccgattac gatcaatctg ctggaagaaa
      241 aagagggatc acgttttagt atagcgaaca aggcaaatcc aacgaatttg acgctatcgg
      301 agatggtcaa aagaggaaaa acctctaaaa aaaatggcgg gttaggtcta tacagtgcga
      361 aaaaaatgtt ggatgtttac gaaaatgcag aactaaaaat cgaattttca aaacagaatc
      421 actatttttt tgtagaaatg tatctggcat ctagaaagag caaatcagcc tactgactta
      481 ctgtcaaagg ataagtttga ggaaagtcta cacggagata gagacaacaa cacagcatat
      541 tcaaaagaaa cagaagcgtg atgaccgttg tcacccttct gtttcttttg ctatgtagtt
      601 aaacaaagta gttgacactt ccgctactta gtattagtac gaaagtatac ttcatgatac
      661 taagtagata agaagtgaaa agcggaaccc acgatcgtca aaatgctaac tgctttgctt
      721 acaccagata aagggaagag caatatcgct tattttaggc gtggagaagc aagcaaatcc
      781 ctacgatcac aaaaaaaatg ctgacccagc agttaagaga gttagaacaa gatgaaagta
      841 tccatcgaaa agcctataac caagtcccgc ctaaagtaga gtactcgctc accgaagaag
      901 gaaaatcatt aagagagatt ctagtagcgt tgtctgtttg ggaagaagaa cgtatcgaaa
      961 aacaaaaagc ctcaagggaa gaagacacgc tactgcatac acatggtggc tacctaaatt
     1021 attgattttc tatgacattt attttaaata aaagaaaaca ggagtttttt taaaagcatc
     1081 atctattttt tatgtaaaaa aatggtatga ttaatcaagt aagaagtgat tggaagaagg
     1141 caagcaaatg aatagtatgg aaaaagtcaa ccagtttaga gatgaacgta attggcgaca
     1201 atttcataac gaaaaagatt tggctatctc tatttctttg gaagcatctg aattgttaga
     1261 actttttcaa tggaaaacgc cggaagaagt aaaagaaaca caattagaac gaatcaaaga
     1321 agaactggca gatgtattga tctattcata catgatggca gacaaccttc acctggatct
     1381 agatgagatc attgaagaaa aattgatcaa aaataatttg aaatatcctg tagaaaaaag
     1441 cgcaggtgta cgaaaaaaat acacagagtt gtagaaatgt gtcattcggc tgttggaaag
     1501 agcaaaagaa aaacagtaaa atgagtctgg agggagagaa aatgaaaaaa aaaggaaaag
     1561 caaaaagatg gctagtcaat atcctcttgt tcctcttgtt attagtagga ctagctttga
     1621 ttttcaatga acagatcaag gattattttg tcagagagac aggagataaa tatgctatag
     1681 cgaatgtaac aaaagaagat ctgaaaaaga ataacgataa agatgtcagt tttgactttg
     1741 atgcggtaga accaatgacg acagaagggg tcatgaggtc gcaaatgaga ggaacagatc
     1801 taccagtgat tgctagtatc gcagtccctt cagtttcaat caatttaccg gttttcaaag
     1861 gcttggataa tacgtcacta ctttacggag caggaacatt atctccagat caagaaatgg
     1921 gaaaaggaaa ttatgcctta gcaagccatc gtgcaacgaa ccctgaattg ctgtttacac
     1981 cgctagaaaa tttagagatg ggtgccaaga tctatttgac agatttagaa aatgtgtata
     2041 catacaaaac attttttaaa gaaaaagttg ctcctacaga tacccaatta ctgaatgaag
     2101 tcgagggaaa agaaattgtc acactaatta cttgtggcga catggacgcc gtcacgcgac
     2161 tagttgtaca aggagaactg gaaagtgtta catcgataaa agatgcgaca gatgatatga
     2221 gatctgcttt caatctagag acaaaaacat tttaggatac tgggacataa gtcgttacag
     2281 attaaaaaca gccaaaggat atgaatcgat aaggaaaaac caatttgctg aaaaaaagag
     2341 aaacgcaaag aggtgcatct ctgatttggt tgttttctag atagacttgt gttccagtt
//
LOCUS       DAXWLG010000236         2397 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXWLG010000236 DAXWLG010000000
VERSION     DAXWLG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.244"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..735)
                     /locus_tag="ACGE4T_002656"
     CDS             complement(<1..735)
                     /locus_tag="ACGE4T_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8387861.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLE"
     gene            796..1536
                     /locus_tag="ACGE4T_002657"
     CDS             796..1536
                     /locus_tag="ACGE4T_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8387862.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1622..2380)
                     /locus_tag="ACGE4T_002658"
     CDS             complement(1622..2380)
                     /locus_tag="ACGE4T_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8387863.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
BASE COUNT          783 a          434 c          420 g          760 t
ORIGIN      
        1 ttccaacgtt tcttttttat tttcctgaag agtaattttt aatgaaaaca gtagactttt
       61 ataatcatgt ttgaattcct ctagatgatg gtaatattct tcatttattt gatacagttg
      121 aagcgattcc tgatgttgtc tttcttttac ttggaaactg atcaacataa tggaaaaaaa
      181 caaagaaaaa aaactgatga ctatcgacaa aagagataga agcagataat tagatgagcg
      241 gaagtgaaca cttcccaata ccaagtaaat cacgccccag cttaataaaa tacaaatacc
      301 tccaaaatag tgttcagttg gataagtaaa aagcaagttg agaaatctcg tctgtttgtc
      361 tatacttttt actaatagat agaaaataaa tgcgataagt agtgcaaaaa aacttgctgt
      421 tccaagatgt ctctttccta aataatccat cagatcaaaa accagaaaaa agctgagtcc
      481 agtgatagca aaaaaaagat tactgatcaa acagctgata aacaggtttt tcgtagtaaa
      541 ataaaaaaga aacatcaata ataagccaac taggtaagga aagctcactt tagggatagg
      601 aagtaaaagt gtcaccacca tcaaagctat acaaccaaag ctgtttttta ctagcagttg
      661 tttcgaaaag aataaataga tacctaaatc taaaatcaaa gtaaaggtca aataaagtgt
      721 cactgttgcg accatcttat tcctccattg ttttgtttca aaatgtacac atattttttt
      781 aataggagcc tgcatatgaa aaagtatttt atcatagaag ataaccagtt tcacagcgaa
      841 tggatcatca gagaattggt caatcatcat atcgagaata tcgtgcagct cacttctatc
      901 tctcaaatca atcagttgat ccagttgggc actattgata atcacgatgt tttctttatc
      961 gatatcgact tgaagcaatc actcacaggt attgatgtcg ccgaacaaat acgtaaaaaa
     1021 aatgcacata gtaatattgt ttttttcacc gcttatacga attttgcagt agagatcatc
     1081 aatcggaaaa tccttccgat tggatatatc agaaaagaca ggaatttatc tgaacaaatt
     1141 cgtcaagtgc tagaacagat caattggaaa gaacagcaga tcgtaaatgt cgataaaatc
     1201 tttattgatc aaaaaggtgg aagtctcgtc ctgtatcctc gggaaatttt atatattgaa
     1261 agtatcaaag gtttgaaaaa ccaagttatg gtcaaaacaa ctacagaaga taaattagtg
     1321 aatacttctt taaaacaatt gaagacagag ttagaaaagt atgcttactt tttactttta
     1381 aaatcttact gtatcaactt atcgcaattg cagaaaattg attctgctca ttatgtgtta
     1441 gaatttttca acggtgactc cttactcgtt ggtcgaaaga tctttgaaaa gacaaaggaa
     1501 cgtttccatg acttccaaaa aactgcttcg agttgatgaa taagagctga gacgtaagca
     1561 tagcagattt cagggagtgg gccttgaatc ggctccactc cctgaaatcg ttgatatgat
     1621 tttacgcttc ctcttctgta ggagcatctg ttaacgacca tgtgaatgtt gaaacgtagt
     1681 tgtctacagc ataattggat acaggaacaa ataattccac tgattgtctc ccttgttcca
     1741 cattctctcc taatgaataa atccatgtgc caatcccttc atttgctcct gcagttacta
     1801 gatctactgc ctgatctgct gggatttcga ttccttcagc gccagttcca gaaacggtag
     1861 atggcattcc actgctatta gttgcgctat ttcgaatcac ggcgtttttg aatttcacct
     1921 ttgaaccagt tagtttattc gtatcagatg ttgtaccaat aaattctgtt cgtttgactg
     1981 ataatttcca accggcataa tttccgcttt tatccgttac ttgtacgaaa ttctgtactt
     2041 gtgtttgtgc tccagtactg tccgttccgc tttgtaatgc agcatatact gtttttgctg
     2101 tacctaactt gattgttcca aaatcaagat ctggagcttg gtcaatcgtc agtaatccgg
     2161 atgttccctg atttggttcg tcaggtgtaa aaggagtatc aggatcattt ggatcaacag
     2221 gaggaactgg aacaacgtct tcaggatttg cagcatctgg tttggtcaca gaaatcgttc
     2281 cttctgtagt ggctgagctc ttattctcag caaaaacact ttgtgtacat aataatagac
     2341 ttccagctag aacggtactt aatgcaattg tttttttcat ttctttttcc ttctttc
//
LOCUS       DAXWLG010000237         2393 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXWLG010000237 DAXWLG010000000
VERSION     DAXWLG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.245"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(177..1361)
                     /locus_tag="ACGE4T_002659"
     CDS             complement(177..1361)
                     /locus_tag="ACGE4T_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFF8387864.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1386..>2393)
                     /locus_tag="ACGE4T_002660"
     CDS             complement(1386..>2393)
                     /locus_tag="ACGE4T_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8387865.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
BASE COUNT          678 a          531 c          384 g          800 t
ORIGIN      
        1 atgccagtct tttccctttt gaccactttt ccaagcaaaa ttccctaaga agatcctgta
       61 aaaaagaagg gaaattgaag ttaaattacc attttgcggt cttctccgca aaaaaaacac
      121 atgcaagaat tccctcttgc atgcgtttca tcttttatcc taacgaatgc tttggcttag
      181 ccgcgcagac gctccacatc acgggcaatc attagttcct catctgttgg gatcactaat
      241 actcttactt ttgattcttt tgtagagata tctacttctt caccacgcaa ttcattcaac
      301 gctggatcga tttcacaacc aaaccaagac atacctttga tgatttcact acgtacatgt
      361 ccgtcattct cgccgatacc agcagtaaag acaattgcat caacaccatt catcgttgtc
      421 acataaccgc cgatgtattt tcggatgcgg tccacgaaaa tgtcataagc taaacgaata
      481 tgttcttttt catagttctt ttcaagatca cgcatatcac tagacaatcc tgttaggcct
      541 agcagaccag atttcttgtt caaaacattg atcatttcat caatatcaat ctctaatttt
      601 tccattaaat atgggagaac agctggatcg atatcacctg aacgtgtacc cattgtcacc
      661 ccagctaatg gtgtgaatcc catagaagta tccatggatt tacctccatc gactgctgtg
      721 atcgatacac catttcctaa gtggcaagtg atgattttta gttcttcgat cggtttgttc
      781 aataattttg ccgcacgttc cgacacataa cgatgacttg tgccatggaa gccatatttt
      841 cttactttga aatcttcata ataagacacc ggtaagctat ataaataatt ttgctttggc
      901 attgttgagt ggaaagatgt atcgaataca gccacactca aaatgtcagg taaaatcttt
      961 ttaaacgctt tgatccccat taagtttgca ggattatgca aaggagcaaa ttctgctaat
     1021 tcagcaatgc ggtccattac tttctcatcg atcactactg aatccccata agtttctcct
     1081 ccgtgaacaa cacggtgccc tactccggtg atttcatcat aagagcttaa aatttgcagt
     1141 tcaatcaact gatctaacag catttttact gctacttcgt ggttttcgat atccatgatt
     1201 tgttcgaatt tttgattatc gccgtacttg atggtaaaaa tagaatcttg taaaccgata
     1261 cgttcgacga tccctttcgc aactacggtt tcttctggca tttgatataa ttgccatttc
     1321 aaacttgaac tcccagcatt gattgcaatt gttttagaca tatgactctc cttctcgttg
     1381 aatgattaca gagcagatga tttccagttt ttaaactcgt tgaaaaattc cgtcaccttc
     1441 tctggttctt ttaatgaagc aagtttcaca agcaagactt cttttacttg ttgtgcttgt
     1501 ggtccttttt tctgtaagat caaaatactt ttttgcgatt gtttattacg gaacaattca
     1561 tctggcaatt gaatcattcc ttgaagataa ccttcttctt taaaccattt tttgatttct
     1621 tcactttgat ccgtttctaa aaatccactc ggcatcaaaa acaaaccaaa accatccggc
     1681 ttcacatatt tcattgattg ctctagcaat aaatgatgtg cataactatg gccttcttcg
     1741 gtacttgtta aaaactcttt tgctttttcg tcattcggat aataaccgat tggcaaatcg
     1801 ctgattgcaa aatcgacagg atctattaat aattcctgca acccgtcttg atgaaagtat
     1861 tgaacatttg cttgtgtcaa atcacttgta gaagctgcga cagccaacaa tgtatcatca
     1921 atatccacac caattccttc tgtttggtac cccgcattcg ataggtttaa aagaactgtt
     1981 aataacaagt tgcccattcc tgccgcaata tccagaatct taaccggtgc ttttttattt
     2041 gtgtatagtt gttcaataag aaaaacaaat aaaaaaccga tactatcagg agtgagttga
     2101 tggttcgcct gcaaatgttc tgtctggctg cccttcaata aaagtaattg agacagtctg
     2161 cgccattctt ctggctcgaa agaaagtttc ttcaactctt cataaagagc tgtgatccgt
     2221 tgcgttgtct cttttgtcgg cactccatcc accacacgta cttgataatc atctattaaa
     2281 ttttctgcat tttcgacata ggcttctaaa aaggaagttc ccaatgcatt ctgtaaaagc
     2341 tgaatagctt ccaggttttg attaaaccct tgttccattt tttctggtaa cat
//
LOCUS       DAXWLG010000238         2389 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXWLG010000238 DAXWLG010000000
VERSION     DAXWLG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.246"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            198..2060
                     /locus_tag="ACGE4T_002661"
     CDS             198..2060
                     /locus_tag="ACGE4T_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8387866.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2060..>2389
                     /locus_tag="ACGE4T_002662"
     CDS             2060..>2389
                     /locus_tag="ACGE4T_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8387867.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          711 t
ORIGIN      
        1 aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt tttaacagta
       61 catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc tataagttgc
      121 acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt atattagcaa
      181 tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt ttgatttact
      241 tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa aatgttagtg
      301 ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag cggaagcaag
      361 tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct gatcgaatca
      421 aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat caaagtctcc
      481 gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact ggaatgaaaa
      541 tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat gtgctggaaa
      601 ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt ttttggatca
      661 taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa gagttcacgg
      721 attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact gaatactttg
      781 aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta atcattcgaa
      841 tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa ttactaaata
      901 ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg attggtcgtt
      961 atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc gtcatgcagc
     1021 aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat atctatagtg
     1081 atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat gtgatttata
     1141 aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg acgaatctgt
     1201 atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa tataatcctt
     1261 ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag caagcagttc
     1321 gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca tatattgcct
     1381 tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga gccgtctaca
     1441 tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat gaagaattaa
     1501 ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg attgctacca
     1561 aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt cctttcatca
     1621 aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata ataaaaaaaa
     1681 tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa acgtccatca
     1741 ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat acgatttatg
     1801 aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa gcttttttgc
     1861 ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg gcagaaggtc
     1921 gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc gaagcagttt
     1981 ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc tcatatataa
     2041 aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta tcgaggaaag
     2101 tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat tagtagaaca
     2161 tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga atgagatgct
     2221 gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt tcaaagaagt
     2281 atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta atttaaccga
     2341 tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataat
//
LOCUS       DAXWLG010000239         2389 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXWLG010000239 DAXWLG010000000
VERSION     DAXWLG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.247"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(3..569)
                     /locus_tag="ACGE4T_002663"
     CDS             complement(3..569)
                     /locus_tag="ACGE4T_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8387868.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(569..1525)
                     /locus_tag="ACGE4T_002664"
     CDS             complement(569..1525)
                     /locus_tag="ACGE4T_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFF8387869.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1525..2181)
                     /locus_tag="ACGE4T_002665"
     CDS             complement(1525..2181)
                     /locus_tag="ACGE4T_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8387870.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          721 a          501 c          443 g          724 t
ORIGIN      
        1 atttatcttt ttttctcaat acaatataaa ataggcggct ggttcttttg gttgataaac
       61 cgataattca gcacattata tgtttcctgt ggcagtttgg aacaaaaaga atcaaccata
      121 tccagttctt tttctccacc ttcatgacca tagtagacga caaggatcaa tcgtccgcga
      181 ggcacaagtc gtttcaatat ttcttccata gctgtgcgtg tagtctctgg catagtgatg
      241 attgctttat cactttttgg gagataccct agattgaaaa tccctgcttt gagattttgt
      301 tgttcagcta tcacttctcc taacgtttcg tggccttgta gaaacaatga gacacgttct
      361 tctaactgac gctgttctag cctttcacga gtagcatcga ttgcttgttg ctggatatca
      421 aaactataga catgaccagt ttttccgatg tgttctgcta gaaaaaccgt atcgtggcca
      481 ttgcccatcg ttgcatctac gacgtgatcc cctggttgca agatttcttg cagtaatgta
      541 tggctgaaat gcaaagcagt ttgcaacatc agacgctcac ctttttccgt acatcatatt
      601 ttccttggaa gctatctctg cgaagaagtt cttcatcaat tgcattcaag acttcccatt
      661 ttttcaagct ccacattggt ccaatcaagg aatcccacgg cgcatcccct gtcaaacgat
      721 ggatgatgat ctcttttggt atcatttcca gctggtcgca aatgacattg acatagtcag
      781 gacggctcat taattgaagg cgaccttcat gatagtcccg caacattctc gttttgcgca
      841 tcagatgcat caaatggagc ttgattcctt ggatatctga atctaaaatg gtgcgtctga
      901 cattttcttt catcatttct aaactttcgc ctggtaatcc gttgatcaaa tgcgtacaaa
      961 cacggatatt atgtttgcgt aatttagcca cgccatctaa ataagtttga taatcatgtg
     1021 ctcggttgat cagttttgaa gtttcttcaa aagttgtttg caatccaagt tctacccata
     1081 aatacatccg ctcgttcagt tcagcaaggt aatcaactac ttcatctggc agacaatccg
     1141 gtcgtgtacc gactgataat cctacgacac ctggtaaatt gactacttgc tcaaatcgtt
     1201 ctttgattat ttcaactggc gcatgggtgt ttgtaaaatt ttggaaataa acgatgtatt
     1261 gttggacatg aggccatttt tgatgcatca tgtcgatttc tttctggaat tggattggaa
     1321 gagggtcttc aggtgcaacg atcatgtctc ccgaaccaga tacactgcaa aaagtacaac
     1381 cgccatgggc aacagtccca tctcgattcg gacagtcaaa tcctccatcg atcggcacct
     1441 tgaaaatttt cccgccaaac tcatttcgta aagcgtaatt ccaagaatga tatcttttat
     1501 ttccttctga ataaggaaaa gtcattaaaa gtccttcttt ctgtaagagt tatctatttg
     1561 ttttcttttt tctctataca ctggataagt caggcacagc caagaaagac tgagtaagta
     1621 gccgccgatg atatcagtag gaaaatgaac gcccagataa atgcggctta tcccgacact
     1681 tactataata aacatcatca gcaattgaag caatgtgcgt aaggttcttt tttgaacaaa
     1741 agctggaagc agaaatagta aagaaccgta aagaatcata ctgcctgctg catgtccact
     1801 agggaaactg taacttgttt caactactaa gtgcgccaat gaaggacgct ctcgcatgaa
     1861 aaatagtttg atcaccgggt tgatgattcc agcaattaca ataaatccag aaacgaacca
     1921 aaatgtttct gccctttttt tcttatacca catccataaa gccgcacaag caaataaaat
     1981 gatgacggta agaggattac cgaatttagt cataaagagt tggtagctgt tcagatcagg
     2041 ataagaagct ctgacaatgc ctgtaaacca ctgatcgaaa ccagaaagcc acgacagatg
     2101 gaactttacg atccagccca aaatgaaaaa caggacaaga aagaagctac cagccacctg
     2161 aaatgatgtt ttatatttca tacaattcct cctttgaagc aatgacttat tatagcattt
     2221 ttggtccagc gaaaaaaacg gtaaagagat aaaaccaatg tatgattcct ctttgaataa
     2281 ccaatattct tataaaaaaa ataactgtaa tcggaaagac attttttggt aatgcaaatg
     2341 aaatgttagg gaaataaaat cagtgttttt cgaaatcctt ttaaacaag
//
LOCUS       DAXWLG010000240         2382 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXWLG010000240 DAXWLG010000000
VERSION     DAXWLG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.248"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(102..709)
                     /locus_tag="ACGE4T_002666"
                     /pseudo
     CDS             complement(102..709)
                     /locus_tag="ACGE4T_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1232..1906
                     /locus_tag="ACGE4T_002667"
                     /pseudo
     CDS             1232..1906
                     /locus_tag="ACGE4T_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1899..2060
                     /locus_tag="ACGE4T_002668"
     CDS             1899..2060
                     /locus_tag="ACGE4T_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387871.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          848 a          366 c          396 g          772 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa ccatatcctt gtaaagttga gttttttatt
     2101 ttaatgaatt aagactatta aatagataat tattgtaact taaaaaacac agctaaaaat
     2161 tagttgtgtt ttttattagg tttacttttc tggtataaaa ggaactagct actttttcaa
     2221 aatttctaaa tcattcaata tgtagcgctt actaaattat tcgccataaa attttataat
     2281 tggatcactc attgttttac ctaagtcata tttaagtaat aaccggtgtt tttacataag
     2341 aagacttcgg gtatttttta gtttgattag ctctgatata cc
//
LOCUS       DAXWLG010000241         2382 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXWLG010000241 DAXWLG010000000
VERSION     DAXWLG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.249"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(28..468)
                     /locus_tag="ACGE4T_002669"
     CDS             complement(28..468)
                     /locus_tag="ACGE4T_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFF8387872.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            626..1390
                     /gene="map"
                     /locus_tag="ACGE4T_002670"
     CDS             626..1390
                     /gene="map"
                     /locus_tag="ACGE4T_002670"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFF8387873.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            1422..2345
                     /locus_tag="ACGE4T_002671"
     CDS             1422..2345
                     /locus_tag="ACGE4T_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFF8387874.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIVPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT          717 a          424 c          487 g          754 t
ORIGIN      
        1 ctggatggct cttttattgc aagtatatta aatttttgcg actagttctt tagcgaaagc
       61 ttctaaattt tcgatatctt cttcttcagc attcagatcc actttaacat tttcggctcc
      121 ttttgatgcg cctgtttttg caaaagcagc atcgaaatca tccaccgatt tgcagaattc
      181 atcgtaaaaa gtatcaccag atccgcagac accgtaaatt tttcccaata gatccaattc
      241 ttgcaagtct tcataaaagt ctacgatctc atcaggcaga tcgccatcat cgtatgtata
      301 agtagctact acgcaaatat ctgcctcctc gaattcatct gcatctactt gggtacattc
      361 gttgatttcg acttcgatat ccagattttc aaatgcttct gcaacaatat cggcgatttc
      421 ttcagtgttt cctgtcatgc tagcatatac gatttttgct aaagccatga tgtgcctcct
      481 cgtttataat tatttaacaa ttatgaatta taccaaaaca tcattgaaaa gaaaataaaa
      541 cctaaagaca cctggggaat gtcatttggc taaatgtatg ccgctatgtt aaaatgagag
      601 ggatttgtaa aaaggagacg aaaatatgat tactttaaaa tcagcacgtg aaattgaaat
      661 gatggcagaa tctggagctt tattagcaga tgtacataaa aatctgcgtg attttataaa
      721 accaggaatc acaagttggg atattgaggt atttgtcaga aactatatcg agagtcatgg
      781 cggaattgct gcgcaaattg gctttgaagg gtatgaatat gcgacttgta tcagtataaa
      841 tgatgaaatt tgtcatggat tccctcgtaa aaagcctttg aaaaacggtg atttggtaaa
      901 agtcgatatg tgtatcgatt tgaaaggcgg catttctgat tcttgttggg cctatgtagt
      961 tggagaatct acacctgaga tcgaccgatt gatggaagta acgaagaaag ctttgtacat
     1021 cggtattgag caagcacaag tagggaatcg gattggtgac atcggtcatg ctatccaaac
     1081 ttatgtagaa ggcgaaaatc tagcgatcgt tcgtgatttt attggtcatg gcgttggtcc
     1141 tacgattcat gaagaaccag cagttcctca ttacggagaa gctggaaaag ggttgcggtt
     1201 gaaagaaggc atggttatca cgatcgaacc tatggttaat accggaactt ggaaaatgaa
     1261 aatggatcct aacggctgga ctgcgtatac aagagacggc ggactttctt gccaatatga
     1321 acatacattg gctattacta aagatggtcc aagaattttg acatcacaag gagaagaagg
     1381 cacttattga tctatccagt caataggtac gggagaggta catgaaagtt tggaacaaaa
     1441 taaaacaaaa tgaaaatctg atgcgcttta ttgagacgac tcaaaagcgc atcactgatt
     1501 cagaaatgag tacgacctct gtcgtcgttg cttattattt actcttgtct ttatttccat
     1561 tgattattgc ttttggtaat attttaccgt ttctccacat cgatcaagag actgtactga
     1621 cctatttacg agaagtgatt cctgagaccg tttaccagtt tatcgttcct gcaatcaaag
     1681 accttttgac acaaagttca ggaggcttgt tatccgtttc tgcattagcg gctttatggt
     1741 ctgcaagtca aagtatcaat gctttgcaaa ttgcgatgaa taaagcgtat ggcgtagaaa
     1801 atagaaaaaa ttttttgatc gtccgtttct tctcactaat tgtcattgtc ttgttcatga
     1861 ttgctattag tggggtaact atcgttcttg ggttgggaca gctgattttg gatgctattc
     1921 agccaatcgt acaaataccc gtgaacttta tcgatcagtt ccaaactttg aagtggccaa
     1981 tcacattggt agctttattt gtcatcatgt tcttgattta tttaattgtc cctaatgctc
     2041 agttgaagtt gaagacgatc attccgggca cgatcttcgc tactgtcggc tggatgctgt
     2101 tatcacaagt gtttagtatc tatgctaggt attttgcaac gaggatcagc ggttatcaga
     2161 tcataggaag ttttattgta ctcatgcttt ggcttaattt tgctgctacg atcatcattt
     2221 tgggcggaat catcaatgca gttgttcaag aatacgtgac gggtcatcaa atcaaaaaac
     2281 gtcgagatcc aagccgaaaa tggttcaata aattgaaaac taaattttct cataaaaaaa
     2341 agtagaaaca agagttctta ttgtaaattt atatcagtaa tt
//
LOCUS       DAXWLG010000242         2361 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXWLG010000242 DAXWLG010000000
VERSION     DAXWLG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.250"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..66)
                     /locus_tag="ACGE4T_002672"
     CDS             complement(<1..66)
                     /locus_tag="ACGE4T_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8387875.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKL"
     gene            371..700
                     /locus_tag="ACGE4T_002673"
     CDS             371..700
                     /locus_tag="ACGE4T_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8387876.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            690..977
                     /locus_tag="ACGE4T_002674"
     CDS             690..977
                     /locus_tag="ACGE4T_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8387877.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1256..1819)
                     /locus_tag="ACGE4T_002675"
     CDS             complement(1256..1819)
                     /locus_tag="ACGE4T_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8387878.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          832 a          364 c          358 g          807 t
ORIGIN      
        1 caatttattt tttattccat ttactttttc tttatgtacg acagtcactt ggcaaacttt
       61 tttcattcat ctcacctcac attcaaacga ttatttgaat gaagtatacc tatttgatca
      121 ctcttttgtc aatctacctt aattccaata cactattata agcaatttca tgtattgaag
      181 taaacttact tgcaagtgcg tttttgattc caagttaaaa acatattatt attctacata
      241 atttcctaat caaataaaac tcctttgaaa attgttctaa aggagtttta ttattttgta
      301 tttctactaa aaatagcgta caatgtaatt actaagtaat tacgaaatgt aaaataatgg
      361 aggaaataat atgattacaa aaacaagaaa acaaggaaat tctattatgt taacggtccc
      421 taaagaattc gatgtgccca atggtgttga agtagaagct aagcttgtag agaatggtat
      481 cctttatgag tttgtagagc ctaaaaaaga attttttgat tttagtgaag atattttggc
      541 tgatattctt tcagaaggtt atagcaaaca agagatctta aaagaattta gaaatagaaa
      601 aaatgcgtta acttctgcat ttaaatctat tgctgaagat actgttgcaa attctaaacc
      661 gatgacaaaa gaggagttgg cagcagaaat tggcttatga gattctacct tctaaacacg
      721 taataaagta tctaaaaaaa ttaaaagaga aaacattaaa agaacaattt ttaacgataa
      781 tttatgatga gatagcagtt cgtcctcatt cgggagaaca aaaaacgggt gatttatcag
      841 gaatttgggc tattggattc aagtatgcag gtacgacata tcgcgttgca tatgaaatta
      901 aagataatgc agtgatacct aacttactat gtggaacgca tgaaaatttt tatgaacaat
      961 taaaaaagat taggtaagaa cccaatgttt caacttgaga ggatagacga cactttaaaa
     1021 gataattttt aacagctgag agttgtaata ccctataatt ttaaaccact aacctatata
     1081 tttatgaaaa atagaaaaaa ttaaaacgca ttttaagcct ctttcaaatt aaaagcttca
     1141 tccttatgca caaagagggc ttaaaatgcg ttattttata ttgtttttta gtaataataa
     1201 aaaaatcact ctaattctcc cataaatatt atagattcct aaagattaaa atagattatt
     1261 ccctattgtc ttttaagtag cggtagagtg ttgccttgct aataccagtc atttccgtaa
     1321 tttcttttac gctataaacc tgacttttat acaattttac tgctcgatca acatctttct
     1381 ggttcactcg tggacggcca cctttacgac ctctcgcacg tgcacttttc aaaccttctt
     1441 tggtacgttc aacaattaaa tcacgttcga actgactaaa tgcttggaag acagtcatca
     1501 ttaagcgtcc gtgaggcgtg ctggtatcga agttttcttt caagctcacc agcttaacat
     1561 ggtgttcttc aaaataggta acaaggtcaa tcaaatcctt tgtactacga cccaatcgag
     1621 aaaaactttc tacaattacc gtatctcccg gacgaatttt atcttttaaa cgatttagtt
     1681 ggggacgaac cgctttggtt cccgtcattt tctcagttag gatttcatta caattttgtt
     1741 cattgagtaa gtctaattga cgatttagtt cttgtgaacg tgtactcact cgggcataac
     1801 cataaatgta tccactcatg cttttcttcc tttcgtactt taattctggc ttgattgtat
     1861 cataaaaggg agttatagat acatagttaa tgcaacaagt tatgatacaa aatatgacct
     1921 cttttattta ataaaagagg tcatttcatt ttgtctcata atcgattgtt tttgatacga
     1981 gtgtgtatac gaaataaaca aaaaaatctt aagtaaattt ctagatacct aatttaatcc
     2041 taactagtgt atcctaaagt taataacgag tttatcacaa tgaaaattat aaaatgctcc
     2101 catattagat ataacatgca atatttgaat tagaactaca aaatttattc gttttataaa
     2161 gtaaataatg ttgagatact aaggaataga atattataag gaattgagct gaatgtattg
     2221 gataaaaaaa gaataacttt attagagatg gactataaaa atgctcgtaa ttttcttctg
     2281 aagtctgaaa gctatttgaa tgctcagcta ccagaatata ttagttttga cgaaacttta
     2341 aaatctgcta aaaaagtttt a
//
LOCUS       DAXWLG010000243         2321 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXWLG010000243 DAXWLG010000000
VERSION     DAXWLG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.251"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            279..491
                     /locus_tag="ACGE4T_002676"
     CDS             279..491
                     /locus_tag="ACGE4T_002676"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFF8387879.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            493..2172
                     /locus_tag="ACGE4T_002677"
     CDS             493..2172
                     /locus_tag="ACGE4T_002677"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8387880.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT          741 a          446 c          475 g          659 t
ORIGIN      
        1 gataaacatt gattttttct ttcaatctag ttgatttcta tacttatttg ctgttttatg
       61 ctagaataac aattaattgt tgtatatttt ttatacagaa ggtacggttg aaaaaatatc
      121 cgaacagata agcaaaaacg aggcttgtct ctttgactcg aatccagtat tctagtcaaa
      181 gcatatgaaa aaccaggtgg cttgtcccct cgttttgtgt tgtctaaaaa aatcaactag
      241 ctaaatcact gctttacgca gataaaggag acaaaacaat gatctataaa gtttattatc
      301 aagaaacaaa agtacgcaac cctaaacggg aagaaacaaa atctctttac attgaggcaa
      361 aaagcgatgt agacgctcgc caacgtgtgg aagaaaatac cccatacaat attgaattcg
      421 ttcaacttct agaaggaagt catctagagt acgaaaaaga aaatgctgat ttctcattag
      481 tggagtttta atatgaaact ttccatcaaa aataacgaga caggcgtctt tgctattggt
      541 ggattaggtg aaatcgggaa aaatacgtat ggggtacaat tccaagatga aatcattttg
      601 attgatgccg ggatcaaatt cccggaagac gatttacttg gaattgacta tgtcattccc
      661 gactacagct acatcataca aaacttacac aaagtaaaag ccttggtgat tactcacggg
      721 cacgaagacc acatcggcgg aataccctac ttgctgaaac aagcaaatat tcctatctat
      781 gctggtcctc tcgcattagc gttgatcaca aacaaattag atgagcatgg cttgcttcga
      841 gatgcagagc tccatgagat caatgaagat acgatcatcc gttttagaaa gacttctgtc
      901 agctttttca gaacgaccca tagtatccca gatgcattag ggatcgtggt gaaaacacct
      961 tctggaaata tcgtagcgac tggagacttc aaattcgatt tcactcctgt tggcgaacca
     1021 gcaaacttgc ataaaatggc tcgtatcggg gaagaaggag tcttgtgcct tttatctgat
     1081 agtacaaatg ctgagattcc aacatttact aaatcagaaa aaacgattgg gcattctatt
     1141 ttgaagatct tttcaaaaat cgaaggacgt atcatttttg ccagctttgc ttctaacatc
     1201 ttccgtttgc agcaagcagc agatgccgct gtggcaactg gtcgtaaaat cgctgttttc
     1261 ggacgaagca tggaaaatgc catcgtcaac ggtcaacgtt taggatatat caaagttcca
     1321 gacggcacat ttgtagatgc acacgaaatc aatcgcttgc ctgccaatga aacgatgatc
     1381 ctatgtacag gttctcaagg agaagcgatg gccgcgttga gccgcatcgc gaatggtact
     1441 caccgtcaaa tccaaatcca gccagacgat acagtgatct tttcaagttc acctattcct
     1501 ggtaatacaa caagtgtcaa tcatttgatc aatttgctgt ctgaagctgg tgcagaagtg
     1561 attcacggaa aaatcaataa catccatact tccggtcacg gtggacaaga agaacaaaaa
     1621 ttaatgcttc gtttgatgaa gccaaaatat tttattcctg tccacggaga attccgcatg
     1681 ttgaaagtac atgcttctct agcacaagat gtaggtgttc ctgcagaaaa ctgcttcatc
     1741 atggaaaatg gcgatgtttt agcgttaact agcgattctg cgcgtcctgc tgggcatttt
     1801 gtggctggag atgtgtatat cgatggtagc gggatcggtg atattggtaa tgtcgtttta
     1861 cgtgaccgtc acctgctttc agaagaaggt cttgtattag ctgtcgcgac agtggatatc
     1921 aagaaaaaag aaatcttggc tggtccagat attctctctc gtggatttgt ctacatgcga
     1981 gaatcaggag aaatgatcaa tgaagctcag cgaatcttat tccacgcttt aagagaatgt
     2041 ttgaatggac ctgactgttc agaatataaa ttgaaagaag cgatgactgg cgcgcttcag
     2101 cctttcttat tcgaacgtac agaaagacat ccaatgattt tgccaatggt aatgacacca
     2161 gatgaagaat aaataagagg ttatgaaatc agcaccttgt cctgaatata agagaaaaca
     2221 aatgaaaaac atgagaagcc atttttgttt gttcttattc cttgatacaa ggtggctctt
     2281 tcatagcctc ttaaatttat ttactttttc atacaaaaaa a
//
LOCUS       DAXWLG010000244         2284 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXWLG010000244 DAXWLG010000000
VERSION     DAXWLG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.252"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(147..371)
                     /locus_tag="ACGE4T_002678"
     CDS             complement(147..371)
                     /locus_tag="ACGE4T_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387881.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            737..1021
                     /locus_tag="ACGE4T_002679"
     CDS             737..1021
                     /locus_tag="ACGE4T_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387882.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1024..1440
                     /locus_tag="ACGE4T_002680"
     CDS             1024..1440
                     /locus_tag="ACGE4T_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387883.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1577..>2284)
                     /locus_tag="ACGE4T_002681"
     CDS             complement(1577..>2284)
                     /locus_tag="ACGE4T_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8387884.1"
                     /translation="TELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRF
                     SFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTK
                     KFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRI
                     EEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
BASE COUNT          776 a          404 c          341 g          763 t
ORIGIN      
        1 ttatttctta tatgcaacat ttacaaataa agtcgctctg agagctcgta tttttcgatt
       61 taagacgttt tatttagttt ttggtgtatt tcattggtcc aagctaaaaa actcttagaa
      121 gcgattttag agccatttaa acccacttaa ttatattctc catgaaattc gctccatgcg
      181 tcaaaagctg cgtctgaaat tccgccgtct tcgttcacta gttgcattaa tttttccaac
      241 gatcgttttc tttcgggtgt catattttta tattccaaca atttttcgtc tgttaaatcg
      301 aacttgattg cttgaattgc ttgctctaat ttttctaagt tttgaaaata ttcgtaagga
      361 aatttttcca ttttttcttt ctccagtctc acaatttttc tagaaataaa atcacttctt
      421 tgccgtctat actctcaaaa aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc
      481 atttttaaat ttggttatcg attacacgtg cagcactccc ccccgaattt taaagagcat
      541 aaggaaacgt gtacccactt tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt
      601 ctcagaaaaa aatttgatat cactttttcg ccatcgtggg tagcttatgt atagcatttt
      661 ggcaaaaaac agctatacaa atatagtatt tgtatggtat acataaatta tacataattg
      721 caaaaggaga ttttttatgg acattttagt tcggaatatt gatccaaagt ttgttgcaac
      781 aatagacaaa cgttgccaag aactcacgaa taaaacagga gaaaaatgga gtcgaaataa
      841 atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga
      901 ccaattcgat ttattgctcg ataaatttat tgctatccaa acagaaaata ctcaaatatt
      961 aagcgagtat gtctacacaa ataatcaaat cattgaaatg cttatcggag gaatgatata
     1021 accatgagaa aaacttctgt ccaaaaacat gttactttgt accctagtca tgttgacgtt
     1081 ttagattcca tcatcgaaaa aaatccagat atcaataact actcggaagc cattcgcttt
     1141 ttatgtatga gttatgaacc cgataccaac acgaccacta ccagcaatca ggcaaaacta
     1201 aatgctatgg gaaaagaaat ttccatcatg actgaaatca tcgcagaatt tgctgattta
     1261 tatctaaaag atacaggcgt tcttattggc acaaattcta aagtatatca agaagtgaaa
     1321 gaaaaaatcg aagctaaact aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa
     1381 gaaaccagtc aaacagtcac ttctagaaat ttcgacccaa caaattttct aaaacgataa
     1441 aaaacagata ggagttattc ctatctgttt tttatttaga agccccatac aatatgctag
     1501 aatgtactag caatcttagt cggttgtaag ggacttgtct acttgcttaa tgtaaggggg
     1561 gcgagttctt ttttatttat tcttctaacc agttacgcag agaaatcttt ggcaaatctg
     1621 acgttaggct tgaaaaagta aattctaatt tggcgatttt attgcccttt tttgctttta
     1681 tttttgtgac tttaagatca ttgaagtatt tttttagttc tcgtaatgca ggatttaata
     1741 ctttttgatc aatgttaccc atttgatagt attctgggat atccattaag ctcctaaact
     1801 cttcaatacg aactacataa taaccagtag aacggtactg cataagcagt cggaatagag
     1861 tttttgcata cgtagaacgc aacgacgtga aagcagataa ttcaaatcta ctaaattcag
     1921 ctgttaaacc attaataata tattctaggt ccggattaac actgacttca acaaactttt
     1981 ttgttttatt gatttcaaaa ccagtaaaca aaacaaagta gcgaatagaa tcctcatctt
     2041 cagtacggta agtaagatta agcatatctt tatagagctt ttctaaatca ccgacaaagg
     2101 cttttgtagc agtcatctta taatcactta actcttttaa ttcctcgaaa gaaaatctaa
     2161 cttttttcaa accacgatct ttcatacgag cgcataatgc aaaaaataaa tccatctgct
     2221 tagcattgaa atttttcagc ggtactaaat tcaattcatt ttgatatttg actgttaatt
     2281 ccgt
//
LOCUS       DAXWLG010000245         2283 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXWLG010000245 DAXWLG010000000
VERSION     DAXWLG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.253"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..335
                     /locus_tag="ACGE4T_002682"
     CDS             <1..335
                     /locus_tag="ACGE4T_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFF8387885.1"
                     /translation="KKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQK
                     QGAVSPFGYEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFL
                     MPANEKNN"
     gene            372..1382
                     /locus_tag="ACGE4T_002683"
     CDS             372..1382
                     /locus_tag="ACGE4T_002683"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFF8387886.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1497..2210)
                     /locus_tag="ACGE4T_002684"
     CDS             complement(1497..2210)
                     /locus_tag="ACGE4T_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387887.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          758 a          383 c          471 g          671 t
ORIGIN      
        1 ataaaaaaga taataactac gtatatctga cttttactac gaaacaaacg gatttcaaag
       61 cattagggaa gatcgtcggt aaaaggcttt ctattgtttc atcagaacgc atggaagaac
      121 aaacgggaca aaagcagggc gctgtttctc cgtttggtta tgaaaaacag attccgatta
      181 ttattgatga agagctctta tcaaatgaaa aacttgtttt tgcaccaggt agaccagatc
      241 aaacgatggt catccaaacg gttgacttaa acaaaatcat ccagctactt gagatgccga
      301 tatttttgat gccggcaaac gaaaaaaaca attaaccaag taaaatagtg catacttgga
      361 ggtgaagcaa catgataagt tatccattat tagaaagaaa tgcaacgatt ggggttactg
      421 caccatcctc aggcgtatca gaggaacacc attcgttact cgaatcagct ttaaagagaa
      481 tggaagaaaa atctttccaa gtagttactg gaaagacgct ttggacgcaa gataaggaaa
      541 aatcaggtcc agcaagaaaa cgagcagatg aattcaatca aatgatgcaa gatgaaaata
      601 tccaaatgat ctttccgcct tggggaggag aattattgat tgagattctc gattttgtcg
      661 attttgatgc tctacaacca aaatggatag taggttattc agatattagt acgttgctac
      721 ttgctgtcac attaaaaaca gggatagcca ctgcacatgg aaccaacctt attgatttaa
      781 gaggagagta cttggatgaa acgacagcaa tgtgggaaag tgtactgaaa acaagagaag
      841 gagaatcgat tctacagcaa ccttctgaaa agtatcaaaa agagtggccc catgagcatt
      901 catcatcttc tgttttccat ctcactgaac caacaaattg gcaatcagtc agcggagatg
      961 cagtaaggat gaagggacga ttattaggtg gctgtattga tacgatcagt catttaatcg
     1021 gtacccctta tggagatgtc caatctttta aaagaaagta taatgatgga aagccgatct
     1081 tgtggtttct agaaaattgt gagctatcgt cagttgattt aagaagatca cttgttcaga
     1141 tgaaactggc aggatggttt gatggcagct cggggatcat gttcggtaga agtgcagcag
     1201 aggatttgtt agattataaa atgaaggatg tatatgaaga tctgtcaaag gaattaaaac
     1261 taccgatctt gtatgatatt gattgtggtc atgtaccgcc acaactgaca ttgatcaatg
     1321 gtgcctatgc agaagtagcg gtagaagata gaaaagcgac tgttttgcaa acgtttattt
     1381 aaaaaatttt ttcctgcctt ggagtttatc aaagaccaac aaatctaaat agaaaagaaa
     1441 atgaaagaag ctgacagtat tttgccagct tctttttgta tgatctatta gaaattttaa
     1501 ttatccataa ctatcacatt gatcagctgt ttcacatcat ttggattacc tgtaggaata
     1561 gctactacag atgcattatc aataatttct tgagttgttt ctctaatgaa cgctggataa
     1621 gatgtttgat ctgcaggcat ttgccaatgt gaaggaactg gataactagt gattgtcccg
     1681 tcatggttac ttgaatacac taaaactccc tggtaactgt atttcccagt gagcataacc
     1741 acatctttcg gatagactgc actccctgca tcataagggg cgataggtgt tcctgcagga
     1801 taatgaataa catgtagttc gaaatcaccc gatgtatctc taaaagtatt acctctaatt
     1861 gctagccaaa cacgtgcgta ctcaatttgt agatcagaat atcctgctaa cggatctatt
     1921 gactgagaag actgtgagga agttgtgtct attgttgaag aagaaatatt tgtctcaact
     1981 gtactactag atgtagaaga agtattgggc tctgagactt tactgcttgt tgaagatgta
     2041 ttcgtttcag tagaagctga tttctgagta cttgattttt cagctgaact ggtttgtttg
     2101 attgaacttt tgtctgtggt cgcagatgga tcactatttt ctttactagg agacgtacat
     2161 cctacaagga ataaagagac caacaataaa ataaaaccta actttttcat ataaaatacc
     2221 tcattatttt tttaatacca gtatagcata atcctgaatt tttgaatccg taaaaccttg
     2281 tct
//
LOCUS       DAXWLG010000246         2283 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXWLG010000246 DAXWLG010000000
VERSION     DAXWLG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.254"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..421
                     /locus_tag="ACGE4T_002685"
     CDS             <1..421
                     /locus_tag="ACGE4T_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFF8387888.1"
                     /translation="LISGKYDYAFDFEGEKDYKSVAVFQSDFEKLKNYAGRRNVNFAR
                     LLEEFYSEELGVGNEYKRETYAKLSHKLSQALKEVTEVKAELDAKLEEVKKYQTERKN
                     NMKNQVEFDEQKEFEKARKRYNKIMNELSYEHLTVHE"
     gene            458..625
                     /locus_tag="ACGE4T_002686"
     CDS             458..625
                     /locus_tag="ACGE4T_002686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387889.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            645..2273
                     /locus_tag="ACGE4T_002687"
     CDS             645..2273
                     /locus_tag="ACGE4T_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387890.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
BASE COUNT          911 a          267 c          433 g          672 t
ORIGIN      
        1 attgatttca ggaaagtatg actatgcttt tgattttgag ggtgaaaagg attacaaaag
       61 tgttgctgtc tttcaatctg attttgagaa gctaaaaaac tatgctggca ggagaaatgt
      121 taattttgca agactactag aagagttcta ctccgaagag ttgggagtag gaaatgaata
      181 caagagagaa acttatgcga aattgtctca taagttatcc caagcgctta aagaagtgac
      241 agaagttaaa gcggaactag atgctaaact tgaagaagtg aaaaaatatc aaacagagag
      301 gaagaacaac atgaagaatc aagtggaatt tgacgaacag aaagaatttg aaaaagcaag
      361 aaaacgctat aacaaaatta tgaatgaact tagctatgaa cacttaacag ttcatgaata
      421 attgagtgag ggtacagaaa aatatacgat cgcagatatg gttaatgaag tggaaactct
      481 tttagcttct ttctttgaaa atggtcacgt aaacgatgaa tggcgaaaag aagaacctaa
      541 aagggttaac gcttatattg ctcgattaaa aaggtttatt gataaatata aggagaatag
      601 tgacaaattt tctaaagata aataaggaga gagtgtgatt acatttgcca gaaatcgaag
      661 gaatattttc gccaaaaata ggtaaaaaac agttagattt cagcagtttt ccgagcgtaa
      721 atagagatta taatctaggt gatgaggtac agatactaaa tattgatttc aaagaactag
      781 acttcgctgt tgctgtttcc aacaaaccca aggcattgga acgagtgaaa aaaatatacc
      841 aagagaatga tcaaaatatc aaaaatatga tcaaaaagta tagacgagat aattcaaaag
      901 ttgaagacgt tttattcaac ggtacggcaa aacatgattt gaatgtttat atggcattag
      961 gagtggcttt gtactgtaat tcaattaatc agacagatat tttggttaat atcgtgagga
     1021 atagctatca aaaaatatta aaaataagta gcgaaacaaa cccttattat gcaatgaaat
     1081 atcttgggga taatttatta aatgataaaa aattgatgca caacactgaa gagctgttat
     1141 ctaatttggc tgcgattaac tttctattca aaacaggttc tgaagattat tttatagata
     1201 acgtactaaa aggtgcttac ggaacagcat tttatccgct ttacaatatg aaacagcata
     1261 aagccgaaca aaaggataac aaacttgaaa ttaaaaagat gaaagaacta ttaggtatag
     1321 gatttgtaaa acccaattca aaagtcacta ttgcttcgtt agcaactact aacaatgatg
     1381 ggaaaatagt aaatgggttt gacgattcag atatagatat attgtcaaaa tcatattttt
     1441 ctttgtatgg agattttgca gatttgatgg gtattgatag ttacttagtc aataattttg
     1501 agatcaaggg tagcgagctg aatgatattg tgtatttcgt tttaatctct ctatatgcaa
     1561 ttgatcagaa taccgagtac cgacaaattg actatttgct agttttttat agtttgttat
     1621 atcagttcgt tttaactcgg ttgtttaaaa atgctaagga agaattgatc ctgaattctg
     1681 attatgaaat agcaaaacaa attgcaagta ttagaaaaga aaacaaatct gaagtaatca
     1741 aattaagaaa agaattaagt attgcgagta aagaaaacgg tcggttagaa tctttagtca
     1801 aatcggcaaa aaaagaagag gaacttttga agcaagagat taaagaatta aaaggaatca
     1861 gaaaagaact tgaacagctg aggtttgaaa ataaagtatt aaagcaattg caggagcaag
     1921 aacatagaca atcattaaat ggtaaaactg aagagccttc actgtctgaa atgatagctt
     1981 atctaaaaca taaaaacatt cttttagtgg gaggacatgt aaattttcaa aataaattaa
     2041 ctgaactatt accaaatatt aaaatgtttg atgtaagcga aaataatgga aagatactta
     2101 aaaatttgaa tacgtgtgat gcgatttttc tatatacaga ttacttgaat catggtctat
     2161 attatcgtgt tatgagtgaa atttctaata aagataagat attttatttg gataatcatg
     2221 tgaatatgaa cttaacaatt aaacgtattt acgatctttt atcacttata taaagagaga
     2281 agt
//
LOCUS       DAXWLG010000247         2268 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXWLG010000247 DAXWLG010000000
VERSION     DAXWLG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.255"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            16..534
                     /locus_tag="ACGE4T_002688"
     CDS             16..534
                     /locus_tag="ACGE4T_002688"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8387891.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            662..1345
                     /locus_tag="ACGE4T_002689"
     CDS             662..1345
                     /locus_tag="ACGE4T_002689"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFF8387892.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1654..1727
                     /locus_tag="ACGE4T_002690"
     tRNA            1654..1727
                     /locus_tag="ACGE4T_002690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1688..1690,aa:Arg,seq:tct)
     gene            1886..2245
                     /locus_tag="ACGE4T_002691"
     CDS             1886..2245
                     /locus_tag="ACGE4T_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFF8387893.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          760 a          350 c          456 g          702 t
ORIGIN      
        1 aagaagttga aaacgatacc tgattacaaa aaaataactt ttataagttc tataacgttg
       61 attatatttt taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact
      121 ccttatatta ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat
      181 gtacgaccga aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat
      241 tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt
      301 tatacaaaag ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt
      361 atcggtagtc ccatctttca gattccatat attggactag tcctttcaaa aacggcattt
      421 gcatggattc aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg
      481 aatacctttg tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt
      541 taaaaacata aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa
      601 cactcatgca gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag
      661 tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg
      721 tacccagcaa aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc
      781 agaagtacca gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct
      841 tcagcctgaa cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc
      901 cacgacaatc ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga
      961 gaaagctaaa aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg
     1021 tcaagcagca gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag
     1081 tgcaaaatca aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg
     1141 cgttttgatg gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat
     1201 tccccgtggc aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga
     1261 tcatcccgat ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa
     1321 ggaatcagaa acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga
     1381 atcgagggct caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga
     1441 tacaggaaat ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg
     1501 ataaccttag aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg
     1561 ccaaaataat tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa
     1621 ttatctataa ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca
     1681 tccgccttct aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt
     1741 agaaactgag gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa
     1801 aggcaaataa aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa
     1861 actcatcaga aaagaggaag aagatatgac gatactatct agaattttag tgactctagt
     1921 tgcaattgaa tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac
     1981 aagtagggtt tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt
     2041 taagaaccaa ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct
     2101 ttcttccgca tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt
     2161 atatggcagt ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt
     2221 ggcattgctt tctattttct tataaatata gaaaaaataa gaatttat
//
LOCUS       DAXWLG010000248         2242 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXWLG010000248 DAXWLG010000000
VERSION     DAXWLG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.256"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            101..1111
                     /locus_tag="ACGE4T_002692"
     CDS             101..1111
                     /locus_tag="ACGE4T_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFF8387894.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            1479..>2242
                     /locus_tag="ACGE4T_002693"
     CDS             1479..>2242
                     /locus_tag="ACGE4T_002693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387895.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFA"
BASE COUNT          750 a          305 c          407 g          780 t
ORIGIN      
        1 ggggtatgcc taaataataa atgtaatacg agtataaaga agaaagcaat tttgggttta
       61 accctattgg ttcctttcag aaaaaggtgg atgaataaaa atgaaaatat taataacagg
      121 tggagcagga tttattggtt ttcatttgtc tagaaaatta ctcaaggaaa ctgaaaatca
      181 aattgtagcg attgataatc tcaatgatta ttatgatgtc caattaaaag aagatcgatt
      241 aacgatactg tacgattata ataattttaa atttatcaga atggatctat tagagaaaga
      301 aaaattagat tctctatttg aaagtgaagg ttttgacata gtaattcatt tagctgctca
      361 agcaggtgtt agatattcta ttgaaaaccc ttatgcttat attgattcta atattatcgg
      421 ttttcttaat attcttgaag cctgccgaaa cttccctgta aagcatctat tatatgcatc
      481 atctagttca gtatatggtg ggaacacgaa agtaccgttt tcaacagaag attcggtgga
      541 tcatccagtt agcctttatg cggctaccaa aaaatcgaat gaattaatgg ctcatactta
      601 tagccacctt tataaaatac caacaactgg gctgagattt ttcactgtct atggtccatt
      661 tgggcgacca gatatggcct atttctcttt taccaagaat attttatctg gaaaagaaat
      721 taagattttt aaccatggaa agatggaaag agatttcact tacatcgatg atatcgtaga
      781 gggaatcact agattaatca ataaagtacc tgaacccaac tccgactgga cagacatcga
      841 tgatttgagt agtgaaagtt gggcaccata taagatatac aatattggaa ataataagcc
      901 ggttgcattg ttggagtttg ttgaaacaat tgaaaagtat ttaggtgtta aagcgaacaa
      961 gatttttttg gacatgcagc cgggtgatgt cgttagaact tatgccgata ttgaagattt
     1021 agaggaaaaa atcaacttta aaccgaatat aaccttggat gagggcatta aaaaattcgt
     1081 tgaatggtat acagtgtatt atttgagtta attttgaaag gaatattgaa gatgggtaat
     1141 tcatacgatt tttttagctt tgttaggtat tttaggaaat tctactttca aacgtgacga
     1201 actttctcgc cgaatagata ttttctataa ctaaaagaat ttctataaaa caaaattgtg
     1261 tatcaagatg tgtgaactgg attctgactg ccaatattga ttgatgagat tgaagaaata
     1321 tatagttgat catggatatc tttatttgaa aagatcttag ctattcacat attcaaaaaa
     1381 atttttacta cgaggactca cacgatacag aggaatttag gtagttctaa ttaaaccaga
     1441 aagtcatcta tagatgtgag ttgagggagg tcatacggtt ggatagtagc agtaataaat
     1501 cacattcaat agctaaaaaa gcttttattt acgcaatagg aacattatca tcaaaaatga
     1561 tatctgtcat tataatccct atctatgcag tatatatttc tgttgccgat ttaggaagct
     1621 ttgattatca aaatacaatt gctcaatttc tttctccagt tatttttgga gcaatatggg
     1681 aagcaatttt acgcatggga ttaggcaaag aaaaaaatat tcagaaaaaa gtagtgacga
     1741 acagtatttg ttttgttata gcaaatacag tgatattagc cattttttta ccaataatat
     1801 atagaatgat tttcccgaat ttagagtatt atcaattgtt tgtttttcaa ttaattttca
     1861 ctcctatcct aagtgttttt caatatattg taagagcact aggtgaatca aagatttttg
     1921 tgatgagtgg tataatcgct gcgggtatca acctgttact ccttactatt tttgttgtta
     1981 caatgggtat ggggatactt ggtctcttac tctctatcat aatttctaat gttataaatt
     2041 tgattttttt gattttcagg gggcagatct ttcaatattt cgcaacgagt tcttttgatt
     2101 ttttattttt aaagtccatg ctgaaatact ctatcccact cactttgaat ctcacttttg
     2161 gatggctaat agttggtttt tcaagatttt atattggaac gtacatcgga gtaacagaaa
     2221 atggtatttt ttcttttgcg at
//
LOCUS       DAXWLG010000249         2223 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXWLG010000249 DAXWLG010000000
VERSION     DAXWLG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.257"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            59..724
                     /locus_tag="ACGE4T_002694"
     CDS             59..724
                     /locus_tag="ACGE4T_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8387896.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(1008..2093)
                     /gene="xerS"
                     /locus_tag="ACGE4T_002695"
     CDS             complement(1008..2093)
                     /gene="xerS"
                     /locus_tag="ACGE4T_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFF8387897.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          727 a          334 c          374 g          788 t
ORIGIN      
        1 aaagaacata taaaaatcgg ttatctacaa tcaaaaaaac ttttcttatt tctttaaaat
       61 ggagttaaga gaaatacgtc caattgacat tcaaaagtgg caaaataatt tattgagcaa
      121 atataaaaat tcttatgtaa gaaacatgta tgggattttt cagatgtcat tagatcgagc
      181 agttgttttg ggactacttg aaaaaaatcc tgcaaaagtt attggaaatg tgaagaaaag
      241 gaaaactaca gttgattttt ggacaaaaga agaatttaca aaagttattt caacttttta
      301 tattgatgat ttgtaccaac attttggttt tatttgtatg tggttacttt ttatgaccgt
      361 tatgcgtttg ggagaagcca ctgcattaac ttggaaagat attgattttg aaaataaaaa
      421 cttgcgtgtg aataaaacac tctattataa gagagccaaa acctatgagt ttgtagaacc
      481 cgaaacggct tctagtattc gtacaatcga actagacgac aatacaatta cctttttaaa
      541 agcttggaag gagcgtcaag tttcaatagg aggcatgtcg tttgtacttt cttataatgg
      601 aacacctact cagaaatata caatccgtca tattatgaga agacatgcaa cgatcgttgg
      661 tgttcatata atccgcatac acggattaag gcatagtcat gcttctttgc ttatatcaca
      721 ataaaaagat gcacttcgaa ttaaagaaag gttaaaacat gctgatgtac aaacgacttt
      781 aggaacttat ggtcatttgt atctaaattc attgagagag ataacaaatg atcccacaga
      841 ttatatccaa actaattttt ctaaaatcaa accgacacct atttacgata ataaaaaata
      901 aatgataaac aaaagtagga tcacaccaaa agaaacacac tagaaaaact ataatacagg
      961 cttttctagc gtgttgtttc ttatttccgc ctatttatac attatactca taaagcattt
     1021 aatgcatcga tcgtagccgc attgtctata tgggtatata aagttgttgt attggtactt
     1081 gaatgtccca attgttgggc agttaatacc aatgaatttg tctgttggta gagtctagta
     1141 gctaatgtat gacgcagttt gtgagggctg acttgaattc taaagccttt tgaatatttt
     1201 gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg gtaaattgct
     1261 agaaataaag ctttttcgtt ttctgttact ttatatcgtt ctttccgaat agctagataa
     1321 ttagctaaat attccattgc gaatgctgct atattgacag catctctttt atttcctttt
     1381 ctagttacta ccacattatt cttatctaaa gataggtcat ctaaattaat atttataacc
     1441 tcggagagtc tcaatccaga tcctaaaatc agtgcattga tcgccaggtc tctttctaca
     1501 tctcgttgat aatagatcaa agctcgttta ctcaaagtat ttttatattc attggcaata
     1561 taatctagat aattttgtgt atccttatct agaaataatt ttttctcaat ggcattggca
     1621 cgtgcagcta acgtagtttt atctgtgatg gttgctactt gatccatagg attattgtct
     1681 aagtatggtt ttcctagagg attattggaa cttttcgaaa gatatttaaa tagaaccttt
     1741 aatgccgtga tatttctatt gatagtagaa gtagatagac ctaagttatt attatctttg
     1801 agcttagttt ttgttgtctc tttttttaca ttttcttttg tgcgacgttt caaatgagat
     1861 ttgtacaagt ttaggtcata ggcagttaac tgtcttatcg aggtgataga agtatcagga
     1921 acgatagact cactcagaag ccattcataa aagagcttat aattttttag atattctaaa
     1981 gtggttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt tatataatcg
     2041 ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt catgtcattc
     2101 atcctttcaa taagtagggg agaagagtat ctctataatt ggactatacc aattatagaa
     2161 tgttttgtca attggtatgc tctaaaattt attaatttgt ttcaaaaaaa gcatataaac
     2221 agg
//
LOCUS       DAXWLG010000250         2215 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXWLG010000250 DAXWLG010000000
VERSION     DAXWLG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.258"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(536..721)
                     /locus_tag="ACGE4T_002696"
     CDS             complement(536..721)
                     /locus_tag="ACGE4T_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387898.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1571..1912)
                     /locus_tag="ACGE4T_002697"
     CDS             complement(1571..1912)
                     /locus_tag="ACGE4T_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8387899.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1912..2142)
                     /locus_tag="ACGE4T_002698"
     CDS             complement(1912..2142)
                     /locus_tag="ACGE4T_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8387900.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          733 a          393 c          301 g          788 t
ORIGIN      
        1 ttttatagaa tagaactagt gttgttttca tagactctcc tttcttcata tttccaatta
       61 atcacaaatt cacggcatta ctagaaaaat taaaggggcg cagaagtgga aaaatactct
      121 tcgactttcc ctacaaacca ggccatcttg tgtttaataa ctataccaaa tctaaggtac
      181 ctctaaatct aaattccatt tgtggcggtg acctccaatt tttaactctt ctatctccca
      241 tccagtatac ataaccttga tacttcgacc acttatatat cgaacacaca aatacctatc
      301 cattgcagga gtactaccta acttagttgc tacttgcatc taaataacag ctgaaaagat
      361 aggacttact cttagtaaag tatctgaact attaattttt aaactatcat ttttatcctc
      421 aactacatgc acatctgttg caacttcact ttctgtctca tccagataaa tgattaagga
      481 atcgggaatt gcaatatctt cagctgacgc tggaatagtt gagctaaatt agtcatcata
      541 tctttattat aaaataacca acagcaaaaa atacaatgaa aaacaaactt ggaactaatg
      601 tatcattaat aaatgttgaa aaattcagca actcattact gtatgatttt agcagcccaa
      661 ccaacatata ccctataaaa gctaataaga tggttaggaa tcctttagaa tttttactca
      721 ttcatagaca cttccttaaa taacttttat tatagaaggt attgtaaata ataatattga
      781 caaaaagtga ctcaaaataa aaaaaaactt acattatact ggaatgaact gtattgatag
      841 tgtacctaaa agctctactg acataagatc gtgtaatcgg taaagtaatt ttattaatta
      901 tacgggatgt aatcaattag agaataaact aatcataaaa ggtactttta cattcataat
      961 tgtcgctttg agattctact ttggttcact atctgaccca attgcacatt ttcgatcatt
     1021 acaagtattg gtgtcgttag aacaactaaa taaaaactag cggtagattt ttcagaatct
     1081 atgctaattt ctgtatggat gttccagtga tggcaaattt ctcttttcat tgtttttctt
     1141 gtcgcatgat atgccttttt gtgttattca gttaaactat agaaattctt tcctagacag
     1201 atcttgatcc caaattttac gtttttgagc atcgatatag tttaaaatga cattactcat
     1261 atctaaagag tttagatttt ttaggactaa aactgtcagt tgttcttttt cttctttctg
     1321 ttaaagtatt gtcgcgactt ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg
     1381 tgacacaaaa aaacgaatga cttgaggaaa tcacctcaat cattcgttta aaatgctata
     1441 tattctttag tttaagatac ggaaattaat gccatcgacc acactagtag aatgaacgac
     1501 atttacttgg ccttttttta atttcgttat gtttagcata taaaaatatt atagagttta
     1561 cttacaatgc ttaaaacata tactgtataa tctgatcaat tttagccatg tcttgcaatg
     1621 gtaagttttc tatttttttg agttttcgtt ccttaaaatc aagagatttt aattgtgtaa
     1681 taattacttg tccttttata tctagctctt caggtaatga gtaacgtgtc ggtaaattct
     1741 ttatagtgga agtaattgga catatgaccg aaaacattgt tttacggtta aattcataac
     1801 gagagacgac tagtcctggt cgtctttttt gaatttcttt tcctacggag ggatcaaaat
     1861 caatccatac aatatcacct tttttaggaa tataaattct ttcttctgac actataaaat
     1921 ttctctccct tcaggagtta aatcttccca ttcgtctttc tcataatact cggcctcttc
     1981 cccaccacta aatggatctt caatttttgg aactaacgta attgttccat catcagaata
     2041 cacaacgata tattcttggt tttctgaagg tttctttccg ttatcagaag gtaaagttac
     2101 cactacagaa cttccttgta atctagattt tgtaacaagc attatcatca ctcccttaat
     2161 aaaagtatac tatataacac taagtaacgc aaaattcccc tttgtaaact atcta
//
LOCUS       DAXWLG010000251         2166 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXWLG010000251 DAXWLG010000000
VERSION     DAXWLG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.259"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            3..431
                     /locus_tag="ACGE4T_002699"
     CDS             3..431
                     /locus_tag="ACGE4T_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387901.1"
                     /translation="MKIKGMRPQLEVELNASYFAADMIYTKVIISNYSTEPAMLVNLE
                     LSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSAISCYLAFEVGKINF
                     NKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(474..653)
                     /locus_tag="ACGE4T_002700"
     CDS             complement(474..653)
                     /locus_tag="ACGE4T_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387902.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(640..978)
                     /locus_tag="ACGE4T_002701"
     CDS             complement(640..978)
                     /locus_tag="ACGE4T_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFF8387903.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(994..1197)
                     /locus_tag="ACGE4T_002702"
     CDS             complement(994..1197)
                     /locus_tag="ACGE4T_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387904.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1212..1958)
                     /locus_tag="ACGE4T_002703"
     CDS             complement(1212..1958)
                     /locus_tag="ACGE4T_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFF8387905.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
BASE COUNT          639 a          420 c          339 g          768 t
ORIGIN      
        1 ggataaaaat aaaaggaatg cgccctcaac tagaagttga acttaatgct tcttacttcg
       61 ctgcagatat gatttataca aaagtaatca tttctaacta ttcaacagaa ccagctatgt
      121 tagttaattt agaactttca tccgctaact taaaccgcag atggtcagct actccattta
      181 agaaattaat tgctaaagga ggatcagtcg atgataatag aatatactct gaagctgtac
      241 cgctaaatat tcctcctaaa agtgctatat cttgttacct tgcatttgaa gtagggaaaa
      301 ttaattttaa taaactcctt aatcaccaaa caaaaatgat atttactcta aatagaaccc
      361 aaatccacaa aattgttgat atcaaaaata caaacttccc aatagagaaa ttagtgaaag
      421 aattaaactg agtaatagta atatacattc tagtttctct tttctattca tttttagtca
      481 gccccctcgg ttggcttttt cgctctgtac tcagcttcag cttcatcaag ccccataaaa
      541 atccaaacca tgtaaacaat cgttcctatc aacgcttgcc tgcttcccca aagtcctaaa
      601 gcgtagatga ttagcggtgc gctgaacact agcgctctat taaattttcc catccgcttg
      661 cctccttaag atttccgaaa aatttgtttc taaaaattca agtgttttac ttcttaaaaa
      721 tagatatgtg tctcttcctt caactggata atagacaaat ccatttttgt ttttctcgat
      781 atcgataata tttctatatc ttgggttctt taaaactcta gaagtaaacc aatcatattt
      841 tctgttaatc cgttctagca cttctggcaa cgtcatccat ctaccagtat catcagcttt
      901 ttttaactcc tcataatcca cttgggagat aattacatag ccttctggaa ttgggatttt
      961 cgcttctaga tattgcatta gctgttcctc cctttacaat tcgtacatag taataatcga
     1021 atctatgatt ctatttgctt ctgcagaagt ctttttaccg tttaaaatta aagataagta
     1081 gcttttgcta attccaaatc tttcagcaag catggtgtaa gttaagaact tcgaactttc
     1141 gacatattct ttgatttttt ctctatctcg ttgagtgatt tctgcaatgt cagtcatact
     1201 aaaactcctt tctaaccaat ttcctctaaa tccatttgag ggtaatagcc ctctgcgatt
     1261 aataagttgt aaataaacac tcgccctttc tgtgtccatt tggtattcat tacaacttta
     1321 gtgccgccat cggctttcgg aatctcactt gtgtgcgatt ttgtgtatcc ttggttcata
     1381 tgttttcggt aaagtatcca ttgtccgcta actttatgtt gaacacctaa ctcgtttagc
     1441 aatttgttta gagcaattgc cgacattccg taatcagctg caatttgaga agtagctacc
     1501 gtatctgtcg atgaaagaat cgtatctaag tagctaatct ttggttcgta ctcggcaatt
     1561 tgttgttcga gtaattggtt cttttcttct aaatctgctg ctaaacgcaa tgcttgtgca
     1621 aaactttgag gtacgtttga atagctacct gtttttctga ttgttggaag gacttcttcc
     1681 attacccagg cctcaaattt ttcagcgctt ggtaagttcg atttaataat caagcggtaa
     1741 acatctgatt ctggaataac tttgaatttt tgacgacgac ctaacgaatc gttaccccat
     1801 gtttcaattg attttttaca atgatcattg gtggctttac tgggattttt gtatcccaaa
     1861 gttttggcaa catcgtttgc tacgaaatac ggaatgtcat tttctagaaa cgttcttact
     1921 tcatgttgtt caaaactaaa aatttgtggt gtgttcattt ttgttcattc ctttctttga
     1981 tataattttc ttatcagtaa gtggtctact gaaataatgc ataaggtggt gaataagtat
     2041 gaataaaaaa actgatgaaa tcgctgcgga tttagttgtt gcatggctaa atcacgaatc
     2101 acaagtatct actaaaggcg aatcaatttc gccaaaagaa attgctcaag cttatttaga
     2161 tattca
//
LOCUS       DAXWLG010000252         2162 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXWLG010000252 DAXWLG010000000
VERSION     DAXWLG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.260"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            44..1054
                     /gene="gap"
                     /locus_tag="ACGE4T_002704"
     CDS             44..1054
                     /gene="gap"
                     /locus_tag="ACGE4T_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8387906.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(1139..2074)
                     /locus_tag="ACGE4T_002705"
     CDS             complement(1139..2074)
                     /locus_tag="ACGE4T_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8387907.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT          638 a          462 c          391 g          671 t
ORIGIN      
        1 gatgccgttt cacagcaccc ctaataaagg aggaactttt gatatgacag taaaagtagg
       61 tattaacgga tttgggcgta ttggccgtct ggctttccgc cgcatcaaag aagtctcaga
      121 tgatattgaa gtagtagcaa tcaacgactt aacaagtcca actatgttag cacaattatt
      181 gcaatttgac tctactcatg gtacttatcc tggcacagtt actgcaacag aagactcaat
      241 cgtagtggat ggcgaagcaa ctcgtgttta tgcagaaccc gacgcaagca aaatcccttg
      301 ggcaaaagaa aacggtgtag atatcgtact agaatgtact ggtttttata catctgaaga
      361 aaaagcaaaa gcgcatttag atgctggtgt caaacgagta gttatttctg ccccagctgg
      421 tgctatgaaa accatcgtct ataacgtcaa tgatgataca ttagatgaaa acgacaaaat
      481 catctcagca ggttcatgta caacaaactg tttagcacct atggcttatt tcttaaataa
      541 tgaatttggg atcgaagttg gtacaatgac aacagttcat gcctacactt ccacacaaat
      601 gctcttagat ggacctgtaa aaggcggtaa tttgcgtgct gctcgttcag ctgctgacaa
      661 tactatccca cattctactg gtgctgcgaa agcaatcggc ttagttatcc ctgagctaca
      721 aggaaaatta caaggacacg cacaacgtgt tccagtcgtt gatggttcat taactgaatt
      781 ggtttcagta ttgaaaacaa aagtaactgc tgatcaagta aacgaagcaa tcaaaaaaca
      841 cacagttgac aatccttcat tcgggtatga tgaccgtcaa atcgtttcta gcgatgtcat
      901 tggaacaact caaggttcga tctttgaccc aacgcaaaca gaagtaacaa ctgctggtga
      961 tttccaactt gttaaaactg ttgcatggta tgacaatgag tatggattta cttgccaaat
     1021 gattcgctta ctagaaaaat tcgctaactt gtaatttgat agatcaatcg ataaaagaag
     1081 cctttgactg gatccagtca aaggcttctt tcttttcatc ccctactttt aaactcgttc
     1141 aatgaacata ttggcttaaa aattgttgga ctacttgctg atatttttca ggatctgtct
     1201 tgtatgcttc cgcatgctcg gctcctttta caacatactt ttcttttggt cccttcgttg
     1261 cacgatagac cttatctaac atttcaaatg gtacaaatgt atcttcgtct ccatgtataa
     1321 agagcattgg gcgctggttt ttcttcaatt gttccactgc atttgcttca ccgaaaaagt
     1381 agccagcacg gacttttgtc actagactcg ttacttgcat aagcggaaat gctggaagac
     1441 taaacatatc gtctagctga taagcaagct catcatgaac ggaagaataa ccgcaatctt
     1501 caatgattgc tttcacatta tctggtagtt tttctccgct agtcatcata actgttgctg
     1561 ctcccatgct gacaccgtat agaacgattt cttgcgattt tccattgttt tctagcactt
     1621 tgtttatcca ctgtacatag tctttacgct ctggccatcc aaatccaatg taatcccctt
     1681 cgctttttcc atgtcctctt gcatcaggaa caaggacatt gtaacccata tcatgaaaca
     1741 tctttgcgta attggtcatc gtttcagcgt ttcccatata gccatgagcc acaagagctg
     1801 ttttttcact tttcgtttca gcaggaagat aaatcgctga taatttcaga ccgtcttttg
     1861 aagtgatggt ccaatcgctt cggttttctt ttgaactaaa ccatttatgt gcatccgttt
     1921 ctttggatgt ctgtgttgta ccaggtgtat cttcgtctaa aaagtctttc tcagaaggca
     1981 caacagcata attataaaaa taattccctg caaacactaa cgcaacaatc agagctaaag
     2041 cgacgcctaa aaggattttc atccaaagtt tcataaatca tccatctcat ttctcttttt
     2101 tcatataacc ttgaattagt ataatcaaag aatctctact atgcaatgtt catttttata
     2161 ac
//
LOCUS       DAXWLG010000253         2153 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXWLG010000253 DAXWLG010000000
VERSION     DAXWLG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.261"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(116..1042)
                     /gene="cysK"
                     /locus_tag="ACGE4T_002706"
     CDS             complement(116..1042)
                     /gene="cysK"
                     /locus_tag="ACGE4T_002706"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFF8387908.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1182..2069)
                     /locus_tag="ACGE4T_002707"
     CDS             complement(1182..2069)
                     /locus_tag="ACGE4T_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8387909.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          593 a          482 c          359 g          719 t
ORIGIN      
        1 gcgttcacta ttctacgtct ctattcaaat aatttttctc atcataaatt tttgtgtgtt
       61 cttttcatct ttaaaagttt tttacataaa cctatctgga gaaaaaccaa gtgccttata
      121 tctcttggta aagggctgta gataaataac gttcaccatt gtcgggaacc acagctaata
      181 ccttcttgcc ttttcctaat tcttttgcga cttttagcgc ggcagcaatt gcagcgccag
      241 atgagatgcc tactaaaatc ccttcttttc gcccaacttc tctagctgtt tccatcgctt
      301 cgtctccact aatagaaagg actttatcat acacagaagt atccagtgtt ttaggaacaa
      361 aacctgtacc gattccttgg attttgtgag gtcccgcttc tttcccttct aaaatagcag
      421 attctgctgg ttctaccccg attaattcga ttttcggata cacacgtttt aattctcggc
      481 cagcgccagt gatcgttcct cctgtaccga tacctgcaac aaacgcgtcc aagccattta
      541 ctccaaatgc ttgatgtatc tcaggaccag tcgtcttctc atgcacaaga ggatttgctt
      601 cattttcaaa ttgtaatggt aaaaagtagc cgttttcttt tgccaaacgt tctgcttctc
      661 tgattgatcc agaaatacca tccgcaccag gagtcaaaat caactctgcg ccgtaccctt
      721 tcatcaataa acgtctttcg atactcatcg tctcaggcat cacgatgatt actttatatc
      781 cttttgcaac tcctaccatc gacaatccga ttcctgtatt tccagaagta ggttcaatga
      841 tagtatcacc tggtttcagt agaccatcgt gttctgcttt ttctatcata cttaaagcaa
      901 tacgatcttt tacacttcct cctggattaa aaaactcaag ctttacaaaa acatctgctg
      961 aatcttctgg tacgatttta tttaatttga cgattggcgt tttgccaatc aattcggtga
     1021 ctgagttata gatagctgtc atcttcaatt cctccttgtc tttgctcttc ttacttatca
     1081 taatacaatc tgttcattta tggcgagtga ttcgtctttg actagcaaaa aaaagacaaa
     1141 gatataccat atatggtaat ttctttgcct ttttatctaa atcaaacgtt tagttcttga
     1201 ccaactaata aagaataact ggacatagac atgccatttt tcgccattag atcatcaaca
     1261 gacatgccaa atttttgtgc aatatcccat agcgtatcac ctgacacgac tgtatatgtc
     1321 tgaccattat ttgtatttac atctgttgtt gaagatgatg acgttgaagt cgtcgtagta
     1381 gtcgttgttg ttgtacttgt ggttgttgta gccgaacctg ttgatggcgt atcaaaacgg
     1441 gttaaattat aagtattaat caatgagttc aatttggccc cataatttgg atcggtagca
     1501 taacgacctg tcaagtacgc tgttgcatca taaaaacttg ttgtattgct tttccacacg
     1561 ccagcataat gataatcgcc cgtggaaaaa ctggttgaac gaagaacatt cgcatggtct
     1621 tggaaggatt cccaataaga agaatatttt cggaatggct cattcatcgt tacccattgt
     1681 ccgtttacat attctttggt tgccatataa acgacctgtc cgttatagct tcctttcaca
     1741 ccgaatagat tataatttgg cgcactagct aatccggaag tgccatatgc actttccaac
     1801 aacgcttgag cgatcatgac tgaagcatac aagtcattac ttgcagcaac ttctgacgca
     1861 gaccagccga tttgatcgat aaacgcttga ggatcagcga ctgtttgaga tccttgttca
     1921 tctgcatgaa catgttgcgg taccgctgta accatcatcg gcccaactaa aagactcgtt
     1981 ccaattaaag gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgc
     2041 cgggcactgc gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa
     2101 taaaaagttc tcttcttaaa acaaacttaa ttaattaaat tatactttta att
//
LOCUS       DAXWLG010000254         2123 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXWLG010000254 DAXWLG010000000
VERSION     DAXWLG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.262"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            271..1821
                     /locus_tag="ACGE4T_002708"
     CDS             271..1821
                     /locus_tag="ACGE4T_002708"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8387910.1"
                     /translation="MSDKNTYACRSQRHKQESDTYENPDQLNETVTSKTANENKQSRI
                     PKKNSNYSKYIFALFTALILVTVATAGYIVYKLKQQEAEAKAKYETAAKNLMASIQEE
                     QDQSRISTKIDTISDGENKCLVYRPVDESAVPFKNANQLLDELAQKQQKKHCEKEVLT
                     VARIKATAISSKVGQYSIEADSFIWDRSKEHFKNPDNISEKAIYVSEKTGKEITNKDL
                     IPDEGSLLGIQQVIQQSILDHAEKPEKIIDAVLTMDRISYDSKMTYTPDELTIILPNN
                     SIGTSKVTLNYKDIAPFIDTDLVSQESIKDALPALDENKKYVALTFDDGPNNSSTLDL
                     LNILKTDNVKATFFMLGQMVDQNPDVAKQAHDEGHEVACHSYSHPQLNALSTDELRSE
                     INEANKAIFKATGVLPRNIRPPYGAIDKKSAETIGMPIIQWDIDSQDWKSKNPRTINN
                     VVKQNVYSGAIILIHDIHPESVKAIPNMISMLKNEGYEFVTIDQLLSGKQKTLHQYFG
                     MNDERLVD"
     gene            1917..>2123
                     /locus_tag="ACGE4T_002709"
     CDS             1917..>2123
                     /locus_tag="ACGE4T_002709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387911.1"
                     /translation="MLILISQSSILVNKKSTGSFVIKNHITKLVEKTFLFIMPLIIET
                     FLKKFIQARYNFISLQNAINAMKIY"
BASE COUNT          828 a          329 c          359 g          607 t
ORIGIN      
        1 aaacatgaca aaaatgtaag gatattattt atgataactt attttctgat accgaaaatg
       61 atgctattat ttttttagac attcagctaa aaaataagag aggaagaaga aatatgtaaa
      121 aaaacctttt aaattggtgt acgtataaaa tgctgagaaa acatctataa ttactacggc
      181 aaaagtaaaa aaatagtgaa aaatgccagg taaaaaataa gaaatttcta tgttttattt
      241 atatgatgat aaatggagga ataaggccat atgtctgaca aaaataccta tgcatgtcgg
      301 agccaacgtc acaaacaaga atctgatacc tatgaaaatc cagatcaatt gaatgaaact
      361 gtaacgtcca aaaccgcaaa tgaaaacaaa caatcaagaa taccgaagaa aaattcgaat
      421 tatagcaagt atatattcgc attgtttacc gcacttattc tagtaactgt tgctactgct
      481 ggctatattg tctataaatt aaaacagcaa gaagcagaag ctaaagcaaa atatgaaact
      541 gctgcaaaaa atctcatggc ttcaatccaa gaagagcaag accaaagtag aatttcgacg
      601 aaaatagata ctataagtga cggagaaaat aagtgccttg tttatcgtcc agttgatgaa
      661 agtgctgttc cttttaaaaa tgcaaatcaa ctcttagatg agcttgctca aaagcaacaa
      721 aagaaacatt gtgaaaaaga agtccttact gttgccagaa taaaagcaac cgcaatctct
      781 tctaaagttg gtcagtatag cattgaagca gatagtttta tctgggatcg tagtaaggaa
      841 cattttaaaa atccagacaa tatttctgag aaagccattt atgtttccga aaaaactggc
      901 aaagaaatta caaataagga tttgattcca gatgaaggaa gtctcttagg tattcaacag
      961 gtgattcagc aatccatact tgatcatgcc gaaaaacctg aaaaaattat tgatgctgta
     1021 ttaacaatgg atagaatttc ttatgatagt aaaatgactt atacacctga tgaattgact
     1081 atcatcttac caaacaattc aattggaact tctaaagtca ctctaaatta taaagatatt
     1141 gcgccattca ttgatacaga tctggtttcg caagaaagta taaaagatgc attacctgcg
     1201 ttagatgaaa ataaaaagta cgtcgctctc acttttgatg atggtccaaa taatagctca
     1261 acacttgatt tgttgaatat actaaaaaca gacaacgtga aggctacttt tttcatgtta
     1321 ggacagatgg tggatcaaaa tccagatgtt gcgaaacaag ctcacgacga aggtcatgag
     1381 gtagcatgtc attcgtattc tcatccacaa ttgaatgcac tttcaacaga cgaattacga
     1441 agtgaaataa atgaagctaa taaagcaatt ttcaaggcaa caggtgtctt gcctcgcaat
     1501 atccgtcccc cttatggcgc tattgataaa aaaagtgctg aaacaattgg tatgccaatt
     1561 attcaatggg atattgactc acaagattgg aaatcaaaaa atcctagaac aatcaataat
     1621 gttgtcaaac agaatgttta tagtggtgca atcatcttga tccacgacat ccatcctgaa
     1681 tcagtcaaag cgataccaaa tatgatctct atgttaaaaa acgaaggata cgaatttgtg
     1741 acgatcgatc aactattgtc aggaaaacaa aaaacattgc atcaatattt tggtatgaat
     1801 gatgagcggc tagtagacta actgttatta gattgcttgg tatgtgtaga agttatactt
     1861 gcaattctct ctcagaaaaa atgttaagca aaacaaattg ataaagagta tcgagaatgc
     1921 taattcttat tagtcaaagt tctattttag taaataaaaa aagtactgga agttttgtga
     1981 tcaaaaatca catcacaaaa ttggttgaaa aaacatttct ttttataatg ccactcatta
     2041 ttgaaacctt tttgaaaaag tttatacaag ctagatataa ctttattagt ttacagaatg
     2101 caattaatgc gatgaagatt tat
//
LOCUS       DAXWLG010000255         2094 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXWLG010000255 DAXWLG010000000
VERSION     DAXWLG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.263"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>2094
                     /gene="acm"
                     /locus_tag="ACGE4T_002710"
     CDS             <1..>2094
                     /gene="acm"
                     /locus_tag="ACGE4T_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFF8387912.1"
                     /translation="LFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIK
                     VKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQV
                     VFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSS
                     SVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIH
                     LGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIEN
                     PEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTE
                     IGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFD
                     PLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTN
                     NWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIE
                     NGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWR
                     YRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWK
                     NFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSS
                     KKSSFAVIIGLLIVT"
BASE COUNT          820 a          343 c          407 g          524 t
ORIGIN      
        1 tgttatttat tattactaat ataacatcac tgattcctgt tcatgtctat gcggatgcag
       61 gcagagatat cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat attacagatg
      121 gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg
      181 attatctttg gatcaattgg ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag
      241 aaatcccatt aatgattgaa aataaaaatg ttggaacttt aacggtcaga aaagattctg
      301 cgcaggttgt gtttaatgaa aatattaaaa acctagactc agtagaaggt tgggggcaat
      361 ttgagataca ggccagaaac gtaaccgata ccggtgaaga aaatacggga ccattcaccg
      421 tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa gccagcttct ggttcttctt
      481 ctagcgtttt ttattataaa acaggagaca tgctacctga agatactaag cacattcgat
      541 ggtttttgaa tattaacaat aacggcactt atgttgaaca accagttaaa atcagtgatg
      601 aaatccaaag tggacaaaga ttagatccaa gtacgtttga aattaatcaa atacacttag
      661 gtgaacagaa ggtttataga ggagaagagg gaatccaaca atttttacaa gatttcccca
      721 gcgctacatt taatttttca gtaactgata attacattga aataactata ccaaaaaact
      781 ttgtcaatct taggaaaata atggtgagtt ataagacgat catagaaaac ccagaacaaa
      841 taaattttga gaatcattcg gaagcttggt ttaaagaatt taataaacct gctgttgatg
      901 gagaaagttt caatcataca gttaaaaata tttcagcttc tggtggagtc aacggtacag
      961 ttagaggcga attgaagatt ttcaaatata tcaatgacac agagattggt atcccaaatg
     1021 tgactttcga attaagacga gcagatgaac agcctattca aggacagtct agtatcctgt
     1081 tgacttcgaa tgaacaggga gaagctagta tcaaaggcct gcaagtagga gactatgttg
     1141 taaaagaaaa agaagcaccc aattggattg attttgatcc actaagttca aatgaactca
     1201 aattctccat aaacgaaaat gatacagagg gagtcagttt acctatttat aacaagaaaa
     1261 aggttacgaa catcacagca actaaaattt ggaacggtgg aaccactcca agaccgagta
     1321 tttattttaa attattcaga gcaagtacaa acaattggga accagttcct gatgcagaaa
     1381 caaaacgatt agacaatgga ataacttcag tgacttggga agatatccaa cagtatgatg
     1441 atagtggaaa cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga aatgattatg
     1501 tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag aataaagaag
     1561 tcatttcagt ctcaggacaa aaaacatggg aagacaatga gaaccaagac ggcaaacgac
     1621 cgacgacgat cacagtaaat ctattagccg acggtcaacc gatccaacac aaagaagtaa
     1681 gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc aaaatacaga gacgggcaag
     1741 aaatcatcta tactgtaacg gaagacaacg tgcccgaata cagtacgacg attgacgggt
     1801 ataacatcaa aaacagttat acaccggaaa aaacaaatat atctatacca ggtagccata
     1861 taacaccgtg gaaaaatttt ccagagaaga cagagagaga gaaaattaga aatctattct
     1921 ctcaaaaatt gcaaatattt gctcatacag gagaatccag cgaacaaaga agcaattata
     1981 ttagtaagag agctgtacct aaacagataa caaataaatc aaaaagcatt cttcctaaaa
     2041 caagtagtaa aaaatctagt tttgctgtaa tcattggttt attgatagta acta
//
LOCUS       DAXWLG010000256         2082 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXWLG010000256 DAXWLG010000000
VERSION     DAXWLG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.264"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            83..406
                     /locus_tag="ACGE4T_002711"
     CDS             83..406
                     /locus_tag="ACGE4T_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFF8387913.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            588..1958
                     /locus_tag="ACGE4T_002712"
     CDS             588..1958
                     /locus_tag="ACGE4T_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFF8387914.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
BASE COUNT          587 a          383 c          427 g          685 t
ORIGIN      
        1 ctcgttgaag taagtatttc tagtttatcc taggattaat tataaaaata aaacattcgt
       61 tgattcgaag gaggttaaac agatgtttga aagttttgat agcagccgca accgttacgc
      121 ttcagttggt gtcgtatcaa gtttgccaga tgaattgatt gacagtatat ggtttattat
      181 tgacctagat cttaagggag tcattccatt agaaaatata ttaacctttg atttgatcga
      241 aaatcgtgga aaagtgacga tgcatttttc tcaagcagga agcaatgtgg aaatggatat
      301 tgatttgccg tttgcctatt cgaaccaatt tcctgctcaa gtatacgcct atgatgacgg
      361 tacgagagaa acgatccttt taccaagtga gattagacaa tattaaggtg atagataaac
      421 gtggatgtcc cttattttac agaccaggcc aagttgttga ttctttgact caattcttgg
      481 cttcttttta tggagtttca taaattatca ttgatttcta attggtattc tcaggataaa
      541 cgctatatac tactaactaa attttaaaga tataaggagt ttaaaagatg cgcgatttaa
      601 caaaaggaag ccccgctaaa ctgattttga tgttcactgt tcctttactg atcggaaatg
      661 tttttcagca gttctacaat atgatcgata tgatcatcgt gggtcagacg ctaggtaaaa
      721 atgcgctggc agcggttggt gcaactggaa gcctgacatt tttgatcatt ggctttgccc
      781 aaggtttaac agctggttta gccattatta ccgcacaacg ttacggtgca aaagattatc
      841 ggggtctgaa gaaaagtttt gctgctagtg ttgtcattag tcttgtcgtc actgtgattt
      901 taactgtcct aagtctcctg tttattcgtc cgatgcttca attgatgcaa actcctcctg
      961 aaatacttga tcaagcacaa acatttattt caattatttt attagggatc tttgcatcag
     1021 tcagtttcaa tttgctttct aatgtcattc gcgcacttgg tgacagccgc acaccactat
     1081 tctttctgat cattgcggtc atcatcaacg tcgtacttga tttgatcttt attatctatt
     1141 ttggcatggg tgtggaaggt gcagctattg ctacagtaat cgcacaagtt tcttccagcg
     1201 tactgtgtct agtgtatatc aagaaaaaaa tgcctttgtt acagttacgc aaaaaagatt
     1261 tcaaatttga aaaagaagag tttgctgtac acctaaatgc tgctttgcca atggcgtttc
     1321 aatcttcgat catcgctgtt ggtgctatcg tgttacaagc agcactaaat agtctaggga
     1381 cagatgtcgt agctgctcaa gccgctgctg gtcgtatcga ccaatttgca aatcagccga
     1441 tgatgtcatt tggtattgca atggcaactt tcactgcaca aaactatggt gcgaaagaat
     1501 atggccgaat cttgcaaggg gtacgtcaaa ctttactgat gagtgtcgga tttagtctct
     1561 tagcaggtgc tatcgtcatt ttcttcggtc atttctttgt tcaattgttt gttagcccat
     1621 cagaaacacg tgtatttgaa ttagcacaaa cctacttcaa tatcaatggg tctctttact
     1681 ggatcttagc agtcctgttc attttacgct acacacttca agggcttgga caaagcaaga
     1741 tccctacgat cgccggtatg atggaattgc ttatgcgttc ttttgcagct atcgttctta
     1801 caggaatgtg gggataccca ggagcagcag cagcttctcc tttagcttgg atcggttctg
     1861 ttgccgtgtt gctttactct tatatgcgtt cgatgaaaca attgaaacac atgggtgaag
     1921 aggaacattt cacattagaa catgcagaag caagatagcc ttgattaaaa gaaatcgttg
     1981 cggctaaaaa tgaaaacttt tattgaaaat atcttcttcg tttaccgaaa acaggttttt
     2041 tagataacaa aaaggctgtg acaaaaaatg tcacagcctt tt
//
LOCUS       DAXWLG010000257         2048 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXWLG010000257 DAXWLG010000000
VERSION     DAXWLG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.265"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..686
                     /locus_tag="ACGE4T_002713"
     CDS             <1..686
                     /locus_tag="ACGE4T_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFF8387915.1"
                     /translation="KKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKAEL
                     LAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNALGN
                     ITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADAMK
                     LPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKDRK
                     KKQGKQKHK"
     gene            768..1817
                     /locus_tag="ACGE4T_002714"
     CDS             768..1817
                     /locus_tag="ACGE4T_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8387916.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            1911..>2048
                     /locus_tag="ACGE4T_002715"
     CDS             1911..>2048
                     /locus_tag="ACGE4T_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8387917.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          575 a          362 c          444 g          667 t
ORIGIN      
        1 aaaagaaaat aatccgattg atgttgatct cgttgggaat cattctgata ctggtagtgg
       61 gctatcggtt gtatttagaa tatcagacag acatccaatt atttttaaat ccgagagcga
      121 gtaaggcaga gctactcgca catattcgtt cgcatgggac ttttgcatcg atactcttga
      181 tcgtattgac agcggtgatg tgtgctgttc caggtattcc tacttcagta gttggagtca
      241 ttgctggttt gagctttgga ccactgacag gtgcgatcat caacattttg ggtaatgctc
      301 ttggtaacat cactgcaatt ttccttatgc atcgcttaaa atttttagat aagaaaaccg
      361 aaacgaatca ttgggtgcaa gcaatccgac aaatgaaaca tccaaaaata ggtgtaatgt
      421 taggctatat gataccggtt attccatctt ctatggtcaa ttttgctgca gatgcgatga
      481 aattgcctct acagcaaatc atattgtcga tcgtgattgg agtcatccct tcgtctgtgt
      541 tgtatgcgtg tggtggagaa gctctgtttc acggatataa caaaactgca gtcacgttgg
      601 ttgcaagtgt tactgtgcta gtagggttag ttgtcttcat ttataaagat cgcaagaaaa
      661 aacaaggaaa acaaaagcac aaataataag tgtggcgaat agttgtattt ttctatttcc
      721 aatcttttcc ataagttgta aactaagata ggaaatgggg gaaatcaatg aagagtaatg
      781 aaaaaacacc tttaacaact tcgcttattt tactattatc aatcacttgt ggcgttgttg
      841 ttgccaatat gtattacatc cagccgattg gaacaaaagt agcagctagt ttatctgtta
      901 gcacaagtgc agtcgggata ttgacgatgt tgacccaatt aggctatgcg ttagggttac
      961 tgtttttagt tcctttaggg gacgtggtgg atcgtccaaa attgatcatt cgaatggtag
     1021 cgttatctgc cgtctctctg ttggccgctt tttttgcgcc ttcgtttacg ttatttgcat
     1081 gcgcttcttt tttaatagga ttactttcga tcgtgccgca aatcatcatt ccttacggtg
     1141 cggtactagc gggaccaaaa gcaagaggga aggtcatggg tacattgctt agcggtttac
     1201 tcgtggggat tttgctttct cggacggtat ctggtattgt tgccagtctt ttttcttggc
     1261 ggatgatcta tttatttgct ttatgtgcag tcggaattct tacacttctg ctttactgga
     1321 aaatgccgcg ttctgtccag caaagtagta cagctgtttc ataccttgat tcacttaaaa
     1381 gtttgccgca tttagttgct tctcaacgtc tattaagaga agcggcagtc agtggatttt
     1441 tcatgtttgg aacattttcg atcttttggt ctacgttgat tttttatatc agcagtcccg
     1501 cctacaattg gggaacattc gaagcaggga tattagcgat ttttggtttg tctggagcag
     1561 ttgctgcacc tatcgtggga cgactggcag attcttattc tgaaagaaaa atcgtgtata
     1621 tgggacttgt tatgcaaaca ataagcttta ttgctttatt ggttggcgga agccatttgg
     1681 ccattttgct tttagctatt attttattag acgtcgggaa tcaatttggt caagtcgcca
     1741 atcaaacacg agtacaaggg ctaggtgaag cagcaagcaa ccgaaataat actgtattta
     1801 cccgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg gtaaatcaac
     1861 aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg aggactacat atgcgttcat
     1921 caaaatatac tgaacattat acagatactt ttataacatt caaggaaatt atgagaactg
     1981 tttctaacat ataccacaat tgtgtaccag ataagattaa aaaccgaaga aatactgatc
     2041 aactgaag
//
LOCUS       DAXWLG010000258         2004 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXWLG010000258 DAXWLG010000000
VERSION     DAXWLG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.266"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(4..567)
                     /locus_tag="ACGE4T_002716"
     CDS             complement(4..567)
                     /locus_tag="ACGE4T_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8387918.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(622..801)
                     /locus_tag="ACGE4T_002717"
     CDS             complement(622..801)
                     /locus_tag="ACGE4T_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387919.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(878..1387)
                     /locus_tag="ACGE4T_002718"
     CDS             complement(878..1387)
                     /locus_tag="ACGE4T_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387920.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(1483..1863)
                     /locus_tag="ACGE4T_002719"
     CDS             complement(1483..1863)
                     /locus_tag="ACGE4T_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8387921.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT          551 a          410 c          321 g          722 t
ORIGIN      
        1 tttttagata ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac
       61 aactttgaag tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact
      121 tagaccgatg acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg
      181 cgaaccgatg ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc
      241 gttgggaaac gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc
      301 taagataggt accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc
      361 aactcctgca actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata
      421 atttggatca gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt
      481 caaaaatggc agttccatat cttttagaat cactaagatc aatggggcga tttctggagc
      541 atcttctttt ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca
      601 gaaaaagaaa tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata
      661 accatgagta acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc
      721 tttatcatta tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt
      781 agggcatttc attttttcca tgttatctca acttcctttc atctttactc tcttagctta
      841 ctcttatatt gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac
      901 ggtcctgttc tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa
      961 gtgtccactc tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg
     1021 cttgcggttg tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt
     1081 ccgtgtcatt tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt
     1141 cacaaaaaaa ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg
     1201 caaaatgaat agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt
     1261 ttttcaatga gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga
     1321 gcagtcccgt cataccggta atccctatta cgatgagggc agcaacacca aacatcattt
     1381 ttttcattct tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga
     1441 aaaatcgaat gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa
     1501 tggcaatccg tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct
     1561 aatcggttct gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc
     1621 tactttgaaa gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc
     1681 attagcaatt ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc
     1741 atctcgttct ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa
     1801 atcacgtgat ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt
     1861 catatgagca ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg
     1921 taaatgtgtc agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa
     1981 aaacagaaag taggtcctcc aaga
//
LOCUS       DAXWLG010000259         1988 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXWLG010000259 DAXWLG010000000
VERSION     DAXWLG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.267"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="ACGE4T_002720"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="ACGE4T_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8387922.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="ACGE4T_002721"
     CDS             850..1038
                     /locus_tag="ACGE4T_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8387923.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="ACGE4T_002722"
     CDS             1051..1608
                     /locus_tag="ACGE4T_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8387924.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="ACGE4T_002723"
     CDS             1640..1882
                     /locus_tag="ACGE4T_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8387925.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          722 a          307 c          406 g          553 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 atttgagaac cgtagcgaca ctcgctgcgg ttctttttta tatattttat atgaagtatt
     1981 ctttattc
//
LOCUS       DAXWLG010000260         1958 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXWLG010000260 DAXWLG010000000
VERSION     DAXWLG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.268"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(135..836)
                     /locus_tag="ACGE4T_002724"
     CDS             complement(135..836)
                     /locus_tag="ACGE4T_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8387926.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(852..1292)
                     /locus_tag="ACGE4T_002725"
     CDS             complement(852..1292)
                     /locus_tag="ACGE4T_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8387927.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1292..1867)
                     /locus_tag="ACGE4T_002726"
     CDS             complement(1292..1867)
                     /locus_tag="ACGE4T_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFF8387928.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          563 a          413 c          299 g          683 t
ORIGIN      
        1 atcatttcag tttacaaatc aaagagtaaa gatttattta tttcaaatat ttgaatataa
       61 aataaccaaa cgatagaaag gcttattcta catcacgtag taatttttct ttcccgtcgt
      121 ttggtttttt atttttagcc taattcttga ccttttgcat gctggttatc cgtaaaatat
      181 tttccagcag gctttccttc caattcagct aatttttctt ggatctgacg gatcaccttt
      241 tttgcatctt ctggattttc tacaaaatcg aatttatccc catccacttg tattttagga
      301 catacatcaa aatcttcata ccattgatta tagcgggtgt tcaacgtttc ataataggta
      361 tataacgtct catcgctttc tagctgttca tagtcacgcc cacgtttttt gatacgttct
      421 agcatcgtgt ccaaagaaac tttgatatgg accatcaaat caggccgttt ttttggagca
      481 acatcatcta actcgtttag catcgtatct aataagctat cgtactgctg tacttcgata
      541 tccgttaccc gacctaaatc agcgtttaga tggaacaaca ggctatcttc atagatggat
      601 cggtctagca cattgtcatc gttcactaaa gcatttttca ttgctaaaaa tcgcttgttc
      661 aaaaagaaga tttgcaataa aaaagcgtac tgctcaggat ttgaataaaa gagcggcaat
      721 acttcgttat cttcgacatt ttcataaaat ggtttagaat caatctcttt tgcaagcata
      781 tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa tcaaaatcac tgacatacaa
      841 cccttcctct tctataagta ctcgcctacg taagtttcaa caggcaatgt ttcaaaatca
      901 tattcacgta cagcttggtc tgattttaaa tacgtatgga gactttgtgt aatcatcaag
      961 ttcgtaggta ccgtctcttc ttgtacaatg atcatcggtt ttttcaacat agttgccaca
     1021 cctaattcgt aagccgttcc tggatcgatt gtttgatgct caaagtcaag gatagcaaca
     1081 acaaattctg catttgtcag ttcttccata tctttttcgt atacttcctg cgcccatcca
     1141 gcgctgaata gttcataatt gctttcttgg tgatgacgtg gagagtagaa acttgtgact
     1201 gttttgtttt cctccaacgc tttttcgata cgacttactc gatcgatttg ctcctctgaa
     1261 aaaaatggtc ctgctaaata tatttgactc attattttgc ctccttcaat aataactgat
     1321 ttttaataga tactgctcgt aattcattga ccagttccaa tggattgttg gaaaagatta
     1381 cttgatccaa ccatttttta tccataaaat cctcttttgc aaaagtttct aataattgtt
     1441 gcaagccgct gtaataaccg ccaatattca atattccgat tggcttgtcg ggcgtcagac
     1501 cgattgctat ccaagaaaga agttgtgaga attcctccaa tgtacctaga cctccaggga
     1561 aaacgaggac agcatctccc ttttcaaata ataatacctt tctttcatcg atcgtttctg
     1621 taggaataaa tgtagtaaca tcttttccag gcaattcagt ttcaaaaagc ccattcggat
     1681 agatacctgt gacttttccg ttattttcca gtactgtttt actaatcact cccataatcc
     1741 cggagtatga accaccgtaa accagttcga tttcttcttg tgccatgtat tttcctaacg
     1801 tttgcgcaat aaatttgtaa ctttctttat taccaaaacg ggaaccgcaa aaaacagtaa
     1861 gtttcataaa aaaacactcc ctaactcaaa agtctatctt cattctacac gtgttttttt
     1921 aaaataaaag actagatttt gtagtttttt agtggtat
//
LOCUS       DAXWLG010000261         1957 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXWLG010000261 DAXWLG010000000
VERSION     DAXWLG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.269"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            572..904
                     /gene="traE"
                     /locus_tag="ACGE4T_002727"
     CDS             572..904
                     /gene="traE"
                     /locus_tag="ACGE4T_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFF8387929.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1523..1864
                     /locus_tag="ACGE4T_002728"
     CDS             1523..1864
                     /locus_tag="ACGE4T_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFF8387930.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          680 a          263 c          417 g          597 t
ORIGIN      
        1 agagagataa tttcattatt taaacaagct aaaaatcttg atattacttc ttttcaggca
       61 atacgaatat tattcgctta cattttttgt ataaatttaa ttgtctgcta tgcttgtgat
      121 gaattgattt tcgttttgca cttttggatg ataaatttgg acaattgaaa atacaaaaaa
      181 aagaaaggcg gtatgaactc ggaaggctgg taaccttcgt cagcgttcat acacggcatt
      241 aagatttggc ccttaatgcg ttaacctttc agtgttacgg tattacccgc aatggattta
      301 gtataacaga tattcaaaag tgataccact ttttttggct gtgttaaggt taaaaatacg
      361 tgatattacg atgaatctat gcctaagcat aatgttataa aaatgtccgt gtggctcttt
      421 gccgtatgac gaacgctgaa attagaggga acgcatgttt ggatgcgttc cctctttttt
      481 gtttgtcatg aacgaaaatc aattctctat ttttgaaaaa taacaagaaa aacttgttaa
      541 aaatatttaa agaaattggg tggtttattt tatgtcatat acaggaattt tgagtctcaa
      601 agatatttgt cactacggta aacggtgtac cgctacagag aaaatcacga agaaactatc
      661 aactggccaa agtaaaaccg ttgtgcagtg taagaaatat ataatccaga aagacaaagt
      721 ctcagaggaa atgatttatt acattgggaa acaaaagcaa ataattttaa aagatcctat
      781 ccccttaaaa gagctatatc caacaatcaa acatgtttat gaccaaaacg gtgtactgat
      841 tggtcgaaga aaaaatggag tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg
      901 ctgaattgat cattctcata acttgtattt tatcgttttt cgcttgcttg aaaatattgt
      961 cgagacttct aaaggaactt agagtcacta gaaaaataag gagtagactt gatctatcaa
     1021 caattccctt cggggagtga cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc
     1081 aatcgagtaa atgcgcttgt ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc
     1141 agagtaaaca agagggaatt tagaagttag acaagttaga atagcgtcta gattaatcat
     1201 gagcagctac taagggatct agactttgac gcaaactcat ggaataagga gtaggtcttc
     1261 tcgtaatggg gaggacgcac aagaaattgt gtcgtgtgct gcagtacatg tagatacttg
     1321 cgaggacgtt gattattgga tcaatcgagc atttaccgct gtctactagg gtttctcgtt
     1381 tcggacgcca taaacgagtc gaaatttttt atgtattttt aagtaaacga aaaacgataa
     1441 aatatgaaca agtcctgaat aaagtgaaaa aattgatata aaacatagag aaaatgaaat
     1501 ttacgtaatg gaggaatgac aaatgaaaaa ttatgatccg aatatacgat tgggcacaca
     1561 tactattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt ttagaagggg
     1621 cggaaattgt aaaggattag atgttttagc attagacgag gatgatttat acgatcaaac
     1681 attgactgat aatccaattg gattcggctt gctacctgaa gatgatgaag gtaatgaatg
     1741 gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag atacttggtc
     1801 atatttgagt gattatattg tcagctttga aatcattgaa tttgtagcgg ataaagaaga
     1861 ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac gacgattaat
     1921 aaaaaggtag tattacctta ggcattgagt aaggaat
//
LOCUS       DAXWLG010000262         1954 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXWLG010000262 DAXWLG010000000
VERSION     DAXWLG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.270"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACGE4T_002729"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACGE4T_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFF8387931.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          699 a          387 c          353 g          515 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaa
//
LOCUS       DAXWLG010000263         1949 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXWLG010000263 DAXWLG010000000
VERSION     DAXWLG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.271"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            430..771
                     /locus_tag="ACGE4T_002730"
     CDS             430..771
                     /locus_tag="ACGE4T_002730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387932.1"
                     /translation="MKANTPYKVGDIFYSSWGYEQTNVNFWQIIKLTEKTAWFRPIKK
                     QTVKTYTSMSGETMPIPNEFIDYPLVRTKDSIVRKRINPALYPNELYGNRSWDTFYRY
                     DNQPVYESSYY"
     gene            971..1264
                     /locus_tag="ACGE4T_002731"
     CDS             971..1264
                     /locus_tag="ACGE4T_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387933.1"
                     /translation="MLRTNAIPIVDSYDRNTNNYLGSFEQTDENILNYVAGLSPFQSV
                     RLVEHTTDTLILTTIGYFFDQVSDQQWLQQILPKLIAKQTGKKTIEAVKIFFY"
     gene            1319..1867
                     /locus_tag="ACGE4T_002732"
     CDS             1319..1867
                     /locus_tag="ACGE4T_002732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387934.1"
                     /translation="MAILQPFGEPIERTEFIQHYMKLFVQVIKHTHQIDEFYSKEIEY
                     LLAEKQKIALLYDYFVEMYDRAPDYFYLSDTLTTNFLAKEYLFASHTKNFMCVEHFVN
                     TYLHLLKTQKICTFEAFQTDYLFILDREAYHAKQAFEKQNQAIEGYPELRIQNNSFLQ
                     QRLLKQLINGFHQRNKGYQKDQ"
BASE COUNT          721 a          350 c          287 g          591 t
ORIGIN      
        1 aaagttttta ttaaaaccat cttgaccttt tgagaaattt caatgcgatt tctttttatt
       61 cccatacttt ttttgttggt cactttcata tacccaatct aagcgatcat cggcgacggt
      121 gcttttacaa cggatggtaa gacaaaacaa ggggcggaaa aacttttttg atcatttcat
      181 aaaaaacttt ttcctacagc attcccttgt tttctcttac cacacgttgt gaacccaaag
      241 cacgccgacg aacgcttagc tatacgaaaa tgaccaacca aaaacaaaaa gtgaggtgaa
      301 tagaaacaaa aagaaattga aatctcccaa aaggtcgccc acctcggaga caacttgctg
      361 ccgaaaatgg ttggttgatt cggctaagta tcaaataagg caaatcaaac attattggag
      421 gaatagatca tgaaagcaaa tacaccatac aaagtaggag acatttttta tagtagttgg
      481 ggctatgaac aaaccaacgt taatttttgg caaattatta aattaaccga aaaaacagca
      541 tggtttcgac ccatcaaaaa gcaaactgta aaaacctata cttctatgag tggagaaact
      601 atgccaatac caaatgaatt tatcgattat ccgcttgtac gaaccaaaga cagcattgtt
      661 agaaaacgaa ttaatccagc cttatatccc aatgaattat acggtaatcg cagttgggat
      721 acattttacc gctatgataa tcaaccggtt tatgaaagca gttattacta agagggaatt
      781 tccctcttag tcaatgttaa aaataacaaa aataaaagga gcaagaacaa tgaaaccaat
      841 aactctatat gaaatcttag gtgaagaacg gtctgacaat ttaccaacaa cgcataaatt
      901 aagagttctt gcttctaaat ctatcaataa aaaatgataa ctgagtattt ctaatcaagg
      961 agagattgca atgctaagaa caaatgctat tcctatcgtt gattcttacg atcggaatac
     1021 caataactat ttaggatctt ttgaacaaac agatgaaaat attttgaact atgttgctgg
     1081 gttaagtcct tttcaatctg tacgtttagt tgaacataca acagatacac tcattttaac
     1141 aacaatcggc tacttcttcg atcaggtatc tgaccaacaa tggttacaac agattttacc
     1201 taaacttatc gcaaaacaaa ctggaaaaaa aaccatcgaa gcagtcaaaa tattttttta
     1261 ctaaatacat gtattcattc tcaagggatt tttcccttga gtgaaaggag aaagaatgat
     1321 ggccatactt caaccctttg gtgaaccaat cgaacgaaca gaattcattc aacactatat
     1381 gaaattattt gttcaagtta ttaaacacac tcatcaaatt gatgagtttt attccaaaga
     1441 aatagagtat cttctagcag agaaacaaaa aattgcttta ctttatgact attttgtcga
     1501 aatgtatgat cgcgcaccag attactttta tttatctgat acacttacta ctaacttttt
     1561 agctaaagaa tatctttttg cctcacacac aaaaaacttt atgtgtgtag aacactttgt
     1621 caatacctat cttcatttgt taaaaactca aaaaatctgt acatttgaag catttcaaac
     1681 agattatcta tttattttag atcgtgaagc atatcatgca aaacaagcat ttgaaaagca
     1741 aaatcaagcc attgaaggct atccagaatt acgcatccaa aacaattctt ttttacaaca
     1801 acgattgttg aaacagctaa taaatggatt ccatcaacga aataaaggct atcaaaaaga
     1861 tcaataattg attctttttg atagtttttt tccatcaaaa acagaaactt ttttttaatt
     1921 gtttgattac tttttcagtc ataaagatc
//
LOCUS       DAXWLG010000264         1928 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXWLG010000264 DAXWLG010000000
VERSION     DAXWLG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.272"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            198..1163
                     /locus_tag="ACGE4T_002733"
     CDS             198..1163
                     /locus_tag="ACGE4T_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8387935.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMQLNEGNGFVKSVGKNGWQIVDQYEENIAYIELLQF
                     HEGKARIDFNPNKIGQFLASSMKNFIHDLFLEPHFSRADIACDMVNVPDEFIMQYRVV
                     DPVSFKPIYGRSGKLESAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQ
                     LRHGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1363..1545
                     /locus_tag="ACGE4T_002734"
                     /pseudo
     CDS             1363..1545
                     /locus_tag="ACGE4T_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          632 a          294 c          437 g          565 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta cttcacctag
       61 ttccgtaact caagttcatt atacgaatat ttttaggttt cttcaagcta gagatttttg
      121 gatgtggatt tttagagtta cgtgaactct ggaaattctt tccaagaatc tcttttttca
      181 ttgaggagga gctatttttg agtaaaaaag gcataatcgt aaatgagtgg gaaaaactta
      241 accgtatttc taacaggcgg ttaaatgttc aaaatttgaa caccccccta aaattacagt
      301 ggtcaatcga ccgaattacc attgttggaa aattgaaaga aaatatttat tatcacacac
      361 caaatgatgt cttgatttta aattttgagc aattgatgca attgaatgag gggaatgggt
      421 ttgtcaaatc agttggtaaa aatggctggc aaattgtcga ccaatacgaa gaaaatatcg
      481 cgtatattga gctcttgcaa tttcatgaag ggaaagcacg aattgatttt aatcccaata
      541 aaattggtca gtttttagcc agttcgatga aaaatttcat tcatgatttg tttttagaac
      601 cccatttttc gcgggcagat atcgcttgcg atatggttaa tgttccggat gagtttatta
      661 tgcaatatcg tgtggttgac ccggtatcgt tcaaaccgat ttacgggcgt tctggaaaac
      721 tagaatcagc ttattggggt agccgtgcca gcgaacgaca aattcggcta tacaataaaa
      781 aattggaaca agaaaccaaa cgaaaagttg taccaaagga agttgagact tggtggcggt
      841 tagaaatgca actacggcat ggcaaggcaa ccgattggca taatatggtt caagaaagtt
      901 tgaatagttt tgcgagcctg cattttttgc cgattgatac aacacctaca gatagaataa
      961 tgctcgctgg tttaatgtct gatcaaaatt tctggtctct tttgtcgagg aattcaaaat
     1021 ataaatatcg ggaattgctg aaagaagaaa gtcaaaatga tgaattgacg aatcatttgc
     1081 gagaaacgtt tgcagaatcg gcagacaatt tgaaacaaga attagacacg tggcttttgg
     1141 gtttggacgt gacagagaag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
     1201 tacgtcaatg tttaatgacg tgagtttacg ataagcgatt attttgcgtg aagcaaaata
     1261 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
     1321 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
     1381 tgttactctt tgtcaaagag taacagaaag gtttacactc tttttattag cgacagcgcg
     1441 aaggaggggt ttaacttggg gtttagtatt tcgttcaaaa agggaacaaa aaatacaagc
     1501 atttctcata ataatcgtga gttaactgat agccaaaaaa ttaatagttt gtctggtaga
     1561 aaataattag cagatttaca agtgggaaat aaaaagctga aagaagaaaa ttgcatttca
     1621 aactacattt tctacaataa ctctaaaatg gcatattttg cgttctgagc ggttttagcg
     1681 tttatttcgt ttagttatcg gcacaatcgg tgaaacacgt tagaggagcg aaaatggagc
     1741 ctgtaaggcg actggtagcg gaacggtcgt gttagattgt gtagccgata gctgaacgaa
     1801 ataaatgctg gcagcgccat gctatttcgg caggaggagg ctcaaggggt tcggggaatg
     1861 aaattccctg aggtcttttt attttttcta gaggagtgct ataatcaaag aaaatgagaa
     1921 aaaagagt
//
LOCUS       DAXWLG010000265         1899 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXWLG010000265 DAXWLG010000000
VERSION     DAXWLG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.273"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            354..602
                     /locus_tag="ACGE4T_002735"
     CDS             354..602
                     /locus_tag="ACGE4T_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387936.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            1105..1899
                     /locus_tag="ACGE4T_002736"
                     /pseudo
     CDS             1105..1899
                     /locus_tag="ACGE4T_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          699 a          319 c          319 g          562 t
ORIGIN      
        1 taaattccta attgtctcct ttaattcatc tataatttca tccacgatat tttaatccgt
       61 tctacaatgc atatctgata tgcattgact gtctaactca tatgtgaatg atttataaaa
      121 tatcccaacg tctcatctaa agaaatgttg atcgtcagca aggagctaaa aaatacgaat
      181 atatttcttt tgtagtaaaa tcggactggt ttgcatatca ttttcttcgt aaatatcaaa
      241 atcatgttaa ctactatcaa ttactaataa aaactaccac tttaagcgtt aagaactgtt
      301 aagtttagtt aagtgaggat agttcacaaa atccattgtt aaacttcacg aaagtggtaa
      361 aactcaaaat gctttatata ggattacagc atcgctcttt cttcgattgc gcgttgggta
      421 aaaaatatta tgaagtaaaa attgacgatg acacgttcct tacggctcat cagatgaaac
      481 agctacaaaa acgaaatgat caactcgaag aaaatttaat tctaaaaaag cgattgccat
      541 attcacgcct cgctcgacga actattaaag gtcaagattt acctgaagat catccttttt
      601 aggaaattga agctttttat gttgcacaat gcatttataa tactacaatg atgttttcac
      661 cagatattct tctagggtgc ggtgtaatga aacagataca cttgcaaaag aaaattcacc
      721 gcgaatttac taaaattatg aataattatg ttaatcttcc gaaatttttt tccaaaatta
      781 agggatcaag ctggtgttat agattgttta aggttggcaa aaaaataact tctttttaaa
      841 caattataga aaaccgatac tctgttaagt tggttgacac ggtgtcgatt tttatgactt
      901 tcttattctg aaacttcact aacggacagt acctaaaaag tctatagtat ggacgtttac
      961 gagtactatt ttatcaaaaa tgctgctcat ttcaacttat atgttttagt gaactacccc
     1021 cacttagctt ggataggcta caattaacta gacagaaaaa ttaaggtgtg tagactagaa
     1081 gaaaacatac caggaggaat ttttatgtct aagagaacac gaagaacttt ttcacaagaa
     1141 ttcaagcaac aaatcgtcaa tctttactta gctggaaagc cacgtgtaga aatcattcga
     1201 gaatatgaac taacggcttc agcatttgac aaatgggtaa agcaatctaa aacgagtggt
     1261 tcattcaaag aaaaagataa tcttacgcct gaacaaaaag aattgttaga actacgtaaa
     1321 agaaaccagc aattagaaat ggaaaatgat attttaaagc aagcagcgct gatattcgga
     1381 cgaagagaca agtaatcgat gcgaataagc atctttaccc tatatcagcg atgtgcagaa
     1441 tattaggtct atcacgtcag tcctattatt atcaatcaaa accaaagaaa gacgaatcag
     1501 aacttgaaga agtagtcgct gaagaattta tccgcagccg aaaggcctac ggctcaagaa
     1561 aaataaaaaa agccttatca aaacgaggca ttcagatcag ccgacgaaaa attagtagaa
     1621 tcatgaaaaa tagaggatta aaatcgagct atactgttgc ttattttaaa gtacatcatt
     1681 ctacttgcaa tgaagccaaa acgacaaacg tattgaatcg taaattctta agagacaacc
     1741 cattagaagc gatcgtaaca gacttgactt atgtacgagt cgggaaaaaa tggaattatg
     1801 tctgtttcat tttggatctg ttcaatcgag aaattctcgg ctattcttgt ggagaacata
     1861 aagatgccgt tctagtaaaa aaagcattta gccgtatca
//
LOCUS       DAXWLG010000266         1885 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXWLG010000266 DAXWLG010000000
VERSION     DAXWLG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.274"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACGE4T_002737"
     CDS             complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACGE4T_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFF8387937.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACGE4T_002738"
     CDS             complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACGE4T_002738"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFF8387938.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
BASE COUNT          513 a          411 c          313 g          648 t
ORIGIN      
        1 aatattgagc aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta
       61 ggacatcttt ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc
      121 tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt
      181 tcttctgttg tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc
      241 gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc
      301 cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa
      361 tcacctggtt tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa
      421 cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt
      481 cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct
      541 gataacacta gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt
      601 tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc
      661 atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa
      721 gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta
      781 tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat
      841 ttcgcatttt tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt
      901 cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa
      961 tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg
     1021 aattctaata cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag
     1081 taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac
     1141 cctgctcctt ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat
     1201 cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca
     1261 tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg
     1321 ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc
     1381 acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa
     1441 tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact
     1501 aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc
     1561 atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg
     1621 aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta
     1681 tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact
     1741 ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa
     1801 gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat
     1861 aacaaaaaaa agaaaaacat tatga
//
LOCUS       DAXWLG010000267         1876 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXWLG010000267 DAXWLG010000000
VERSION     DAXWLG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.275"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(215..475)
                     /locus_tag="ACGE4T_002739"
     CDS             complement(215..475)
                     /locus_tag="ACGE4T_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8387939.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(502..1803)
                     /locus_tag="ACGE4T_002740"
     CDS             complement(502..1803)
                     /locus_tag="ACGE4T_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8387940.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT          693 a          396 c          293 g          494 t
ORIGIN      
        1 aataataatc tttatgtaaa aaagggctgt gacaaaagcc ttgtcacagt cccatattcc
       61 gaataaacgg tggtcaaaag cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa
      121 gccaacattt cacaaaaatt taagaagcaa ttttcgtaaa tttcttctta tttcttgaag
      181 ctaaccactt ctgttacaac cacttttaga acatttaaag cttcgatgtg acaaaccatc
      241 tatgctgctc gatcataaaa ttgatagcaa aaattccaca aagtacatat gtcccccatg
      301 aaaacaaaac attcatatgc ggtacaatga tctttggaag aatcacgatc aaaaatgatg
      361 ccagtgccca aatccatcga ttgatttgaa tatgatatgt agctagcata ttcaatataa
      421 ggtgactagc cgttaataaa aagataaaaa caaaaaataa aatcagccaa tacattgttt
      481 cccaccttct aaaaaccaaa cttagacagt ctggacttcg ttttctccac gactttgttc
      541 atctgacagc atctttttat cataagcttt tacaaatgga taatagataa taaatgctat
      601 caatgtgttg atcatgatca agataacaac acgccagtca tttcctgctg aaagataacc
      661 gccgattgga gctggtaatg tccatgttgg caaaacagta aatctattga ccatttctaa
      721 tttgactgca aaataactga taatagtcaa aactaaagga actaagttga acgggatgaa
      781 catgtaagga ttcaacatca taggagctcc aaaaattatg ggctcattga tattgaagat
      841 accaggaata atagaaaaac gaccaacctc tttcaagaat tttgatttac agaagaatat
      901 aaacaaaaca gataccacga tcgttgctcc tgcaccacca attgttaccg tccattgata
      961 aaactgttca ggagcgatat agggtaattg ggtggcagct gtaccatctg ccaatgcctt
     1021 tgcattctca tccaacatga tcagccacat tggacgaaca acagccccta caatactcat
     1081 gccatggata ccaaatgacc acaacaaatg gatcaaaaac attggtagta aaacaccaag
     1141 caaattattc cctaaaatat tggttaaagg ctcgaataac gataataagg cagcgtttac
     1201 atcaaagccc gcgacgcttc gaatcagcca agtaattgtt aaaatgactg cacctggaat
     1261 caatgcctca aaactacggg caacagcagg tggcacttgt tctggcattt tgatagtaat
     1321 atttcgattt ttaaagaaac gatatacttc cactgcaaaa atcatagata agatccccgc
     1381 aaacatccca gatgctccca aagaactcat aggtaagacc cagccaagag gtgcctcttc
     1441 aggagttaaa acgtttacag gaacttgtaa cataaagaac gttcctaaag ataatatacc
     1501 acctgtcaca ctatctaatc catatgattt agctagatcc gaacccataa caaaagttgc
     1561 ataaatagcc atcaatccca tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg
     1621 ggcaataaat tcattccatc caggaaacgg taaattgata aagaccataa aaaatgtccc
     1681 aatcaaaatc aatgacaatg ttgaaacaac cccattacga attgctaata aatgacgctg
     1741 tgtaccaatt tttgccattg gtgggacaag tttctcttcg acccatttca tcagcttttc
     1801 cactaaaacc actccttttc aataaaatat gaaccattca ttgcatgaca acgcaaagaa
     1861 tgttcataac taatta
//
LOCUS       DAXWLG010000268         1861 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXWLG010000268 DAXWLG010000000
VERSION     DAXWLG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.276"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..665
                     /locus_tag="ACGE4T_002741"
     CDS             <1..665
                     /locus_tag="ACGE4T_002741"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8387941.1"
                     /translation="YLKTEKVLPAETAADVAGGSGRFLSLAKEINRYDLIDFSSGMLE
                     NAKKEAEKNELPNVSFIKQSLEEFIQSNETYEMVFTAANPALVHLIQLEKLMKKSSKW
                     CVIIRVVESIDDVFYPIEKQLGMIEEDPNTSPVVMDQFETYLIDEGFKVKKKDFTYFT
                     QESITEELMKLYYDAYAKDPVVTRYIRKLFSEKREILSTTKLTYRLLLWSADKKRTFH
                     DS"
     gene            complement(687..1841)
                     /locus_tag="ACGE4T_002742"
     CDS             complement(687..1841)
                     /locus_tag="ACGE4T_002742"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFF8387942.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          574 a          366 c          351 g          570 t
ORIGIN      
        1 attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt gctggaggat
       61 ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc gatttttctt
      121 caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca aatgtttcct
      181 ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa atggttttta
      241 cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg aaaaagtcaa
      301 gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt tatcctatcg
      361 aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta atggatcaat
      421 ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt acttatttta
      481 cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac gcaaaagatc
      541 ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc cttagtacga
      601 ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc tttcacgact
      661 cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc attttcgatc
      721 tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg ttcaggaagc
      781 gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga atctagcatc
      841 tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata acctgtaaaa
      901 aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc tggctgtagt
      961 gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt gtaaagatta
     1021 ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg ttcctgattt
     1081 tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt agtatacgga
     1141 atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc tttatgtcga
     1201 ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc atcaacaaca
     1261 ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag aaatacgctg
     1321 tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac agcaccgaca
     1381 ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact tgctatgact
     1441 agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa agctgcggtg
     1501 ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat tttcagtaaa
     1561 tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt tccgacgatg
     1621 accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc atttgctcct
     1681 agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc aatccctata
     1741 tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc taaatataaa
     1801 ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc tctttactta
     1861 t
//
LOCUS       DAXWLG010000269         1859 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXWLG010000269 DAXWLG010000000
VERSION     DAXWLG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.277"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(404..1507)
                     /locus_tag="ACGE4T_002743"
     CDS             complement(404..1507)
                     /locus_tag="ACGE4T_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFF8387943.1"
                     /translation="MANKNGYDIEMSYVVAPGETLKEVAEELGLSHKDLAERLGITPK
                     TISKIVNGTAPITPETALKLERVLGISSNFWNQLELNYREKLTEIEAEAKLKDEIEIL
                     KEYIPYNEIANAGFVPKTRNLKEKVENTLNYFGYSSLKIFIESVNDNQLLAGAYRIKE
                     VDKVNKLALLAWIRAGELEAQEIEAEIFSKEKLKNSLSELRNLTLQSNPSIFIPKLQK
                     ICASFGVKVVFIPEVKGSKVCGLTRWLTPKPQAIIQLSLRYKSNDNLWFTFFHELGHI
                     LKHNKIPFYTSSNEYIDSIEEQEADEFSANTLIPRDSYNDFVEQGCFTRNSICNFAEM
                     ISIHPGIVLGRLQKEKYVQWNQYNDLKTWYSWK"
     gene            complement(1497..1826)
                     /locus_tag="ACGE4T_002744"
     CDS             complement(1497..1826)
                     /locus_tag="ACGE4T_002744"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8387944.1"
                     /translation="MIVRARKRKLQKILADSKLINLEFGKQIGDKVKQRIAELLAAET
                     LEDISHLPPTKLHSLSGEYEGCFAVKLTGNFRLVFKAYTCEEEFTTQKSNTEIIVIEE
                     VVDYHGK"
BASE COUNT          556 a          367 c          266 g          670 t
ORIGIN      
        1 ctttagcttg tataatactc ttcaaaaaat tctaacacag aacaattcaa ttggctacat
       61 taatgtaccc tgtaggactc gagcctacga ccggacggtt atgagccgtc tgctctaacc
      121 aactgttaat cagtgttcca aagggagtga ttatactctg ctattttcaa actttcttaa
      181 atcgtctatg ataatctcag tttatggtgg caaaatggtg gcaagaaaaa aagtgagtat
      241 gcccgctact tgcgcaatcg gagtactgct cgatcattta tttggaaaca ggctacactt
      301 gagggtttgg aagagtcaaa gcggttgagc ggtgaacgga aaaagaactt aactacgtat
      361 agttaggttc ttttcgttta tgttttatcg gtagaataat tttttacttc caagaatacc
      421 aagtcttcaa atcattatat tgattccatt gtacatactt ttctttttgc aaccttccta
      481 aaacgatccc cggatgaatt gagatcattt ccgcaaaatt gcaaattgaa tttctagtga
      541 aacaaccttg ttcaacaaaa tcattatagc tatctctagg tattaatgta ttagcagaaa
      601 attcatctgc ttcctgttct tctatactat caatatactc atttgaacta gtataaaaag
      661 gaattttatt atgctttaat atgtggccta actcatggaa aaatgtaaac cataaattat
      721 cattagattt gtatcttaaa ctaagttgta ttatagcttg cggtttgggg gttagccacc
      781 ttgttaaccc acacacttta ctccctttta cctctggaat gaatactacc ttcactccaa
      841 aactagcaca aatcttctgt aatttaggaa taaatatact aggattggat tgcaaagtaa
      901 gatttctcaa ttcagataaa gaatttttta acttttcttt agaaaaaatt tctgcctcaa
      961 tctcttgagc ttctaattct ccagctctaa tccacgccaa caaagcaagc ttatttactt
     1021 tatctacttc tttaattcga tacgctcctg ctaacaattg attatcgtta acactttcaa
     1081 taaagatctt taatgaagaa tacccaaaat aattaagcgt attttcgact ttttccttaa
     1141 ggttcctagt tttaggaaca aatcctgcat ttgctatttc attatatggt atatattctt
     1201 tcaaaatttc gatttcatct ttcaacttgg cttctgcttc aatttctgta agcttctctc
     1261 tgtagttaag ctccaattga ttccaaaaat ttgaagatat gcctaaaact ctttctaatt
     1321 ttaaggctgt ttcaggtgta attggcgcag ttccgttaac gattttagaa atagttttcg
     1381 gtgtaatccc taacctctca gctaaatctt tatgagataa cccgagttct tcagctactt
     1441 ctttcaatgt ttctcctgga gcaactacat aggacatttc tatatcgtac ccatttttat
     1501 ttgccatgat aatccaccac ctcttcaatt actattattt ctgtattact tttttgtgtt
     1561 gtaaattctt cttcacatgt ataagcctta aaaactagcc taaaattacc cgtcaatttt
     1621 acagcaaaac acccctcata ttccccagag agagaatgga gtttcgtggg cggaagatgc
     1681 gaaatatctt ctaatgtttc tgccgctagc aattcagcaa ttctttgctt aactttatct
     1741 ccaatttgtt ttccaaattc taaattaatt agcttggaat cagctaatat tttctgtaat
     1801 tttcttttcc tcgctctaac tatcatccga cttaaccttt taggttaaca actctaaat
//
LOCUS       DAXWLG010000270         1848 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXWLG010000270 DAXWLG010000000
VERSION     DAXWLG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.278"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            175..372
                     /locus_tag="ACGE4T_002745"
     CDS             175..372
                     /locus_tag="ACGE4T_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387945.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            478..960
                     /locus_tag="ACGE4T_002746"
     CDS             478..960
                     /locus_tag="ACGE4T_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387946.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1018..1614)
                     /locus_tag="ACGE4T_002747"
     CDS             complement(1018..1614)
                     /locus_tag="ACGE4T_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8387947.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          579 a          278 c          367 g          624 t
ORIGIN      
        1 tttgaaaaaa attcttctaa atacaacagc tgttttaatt gtgaaaacga aaacagtacg
       61 ctataatgcc tgtaaggttg atttttaact gtaaataaaa atcaatgagt taaatctcca
      121 aagaaatagc agtggtaatg tggagaaaag ttccagcagt ttaggaggat tttgatgacg
      181 gaagaaatga ttaatttagg agaacaatat gcatgcaaac caattggttt tacaaaaacg
      241 gtcattggtg aagttgtatc aaaaatgaca aactgcgccg tggtcaaagt tgcccaatgt
      301 gctgcagagg accaagaatt attagatgaa aaagcaagca tggttgttgc aaaatatgat
      361 acatttgaat aaagaaaaca attggacgtg cggcgtgctg caagtccaat ttctttgaga
      421 aggtaaatca gtattctttt cctattttct ggagaagaaa aagcgaggcg agttgtcatg
      481 gtaacattct ttacggttct attgttttta ttcgttgttg tgatgttttt atttctttgg
      541 ctggttcgaa aagaaattat ttatcggact gtgcgaaatc gatgggtcta cttggtgata
      601 cccttcttag tgtcactagt catttggtat acactcatca gtcatcctac agcagatgaa
      661 ttggcaaaag ggatcttgtc agcaatgatt tttattagtt ttttgttgga cagtagagga
      721 atcacagaag aaggacttgt tttgaatagc tttgacaaaa aaggtgttcc tttttctgag
      781 atcaataaaa ttgtattata tcagccaaaa ggttctaaaa tcgtaaaaat gaattttttc
      841 cgaaacggat ggcgaggacc gatgctgaaa ttttctgcat cattagaaga attagttcca
      901 tttttgtccc aacgcttaaa cgatgaggca gagattgata ttatgattga tccagaataa
      961 aaacagaggt tgcgacaaga tgtttgtcac aacctctgtt ttttatttca aataaaatta
     1021 tccaaatacg cggaagcgat cggcatcgtt catgtattct ttttccccag ccggtgtttc
     1081 tggtgaaccg aatactaatt gcgaacgtag tttccagttt gaagggatat tccattcttt
     1141 tgcaactgct tcgtcaataa ctgggttgta atgttgcaag tttgcgccca atcctttatc
     1201 aactaatgct acccaagtat tagctgttgc gattccgttt gattgttctg accaatctgg
     1261 gaagttgtct gcatataatt caaattgttc ttgtagacct tttaccacgt cagtatcttt
     1321 gaagaataat actgtaccat aaccattttt gaaaccagct aatttgtttt gtgtatttgg
     1381 gaaagcttct gctggagtca atgggcgaag tgcttcttca gtgatttccc aaagtttttc
     1441 atgtgcatct ccgaaaagga taactgcgcg tgttgattga gcattgaaag cagttgggct
     1501 ttctttaatt gcttctttga tcaaagttgt taattcttca ttagaaagtg aaacattacg
     1561 tcctagatga tagattgaac ggcggttttt caaagtgtca gtaaaagttg tcataataaa
     1621 atttctcctt tgatttgcgt tttgcatttt tttaattgat tacgaaatga agtatagcac
     1681 cttacaaaaa gtaagtaaac agatatgctc acaaaaaata agcgaagttc aaaaaacaat
     1741 tttaataaga aaaatcgtta tgtaaggatt ttcttagtta tcgctttcat tgttgtgttt
     1801 taggaatttt tgcttatttg aaaaaaacag ctgatatata attattaa
//
LOCUS       DAXWLG010000271         1833 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXWLG010000271 DAXWLG010000000
VERSION     DAXWLG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.279"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..244)
                     /locus_tag="ACGE4T_002748"
     CDS             complement(<1..244)
                     /locus_tag="ACGE4T_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387948.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIK"
     gene            579..>1833
                     /locus_tag="ACGE4T_002749"
     CDS             579..>1833
                     /locus_tag="ACGE4T_002749"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8387949.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYS"
BASE COUNT          578 a          294 c          330 g          631 t
ORIGIN      
        1 ctttaatgta atctacttca tgagcaggta attgaatgac ttttctacca cattttttct
       61 tattttcttt atactggtgc caataatcaa gagcagtctt gccttgcttg aacttattga
      121 ctacattata aatagtttgt atagcacgtc ccattcggtt agcgatttca accggcttat
      181 tatgttgaag ataatatgat tctatcattg tcagttcgtc tatggtaaga tgtttgtagg
      241 tcatttatgg ttacactcct ttgttttctt tcgtcggaaa tacaatttga gtgtaccata
      301 aatgcctttt tatttttcta acttaatttt acaattcgcg ttatatgaat attgtaactt
      361 gtgaataagg aattagcagg agaatactag ttcagcataa aggaagtgat gggacttctg
      421 agacaacttt ctgttatgca gaaatacaca tataaacaga caataaacgc ttttccccgt
      481 ttcttttaat ttcctttaca atttggttat tcactaggtt tttaaacttt gctatggtaa
      541 gatgatagag gcattatttt atggttagga gtgtatatat gaataaaaat ggggagtgga
      601 atgcggcacg cagaatattc attattctta tggatgtact tgtattaaat gtatcgatct
      661 atcttagttt tttgctgaaa ttccagggag aagtaccagt gagaaactta gaaacctttc
      721 agcattcagc aatttttatt tccatcatct ttattgcgtt gaatattttg ttaggagctt
      781 atgtattcta taaccgaatg atcagtgata ttgtcttcat tactgttatt ggacaagtac
      841 taatagcatt ggggatcatg gtagttactt ttgctggccg atggtttgca tttccgcgtg
      901 gcgtcatttt gatctcgttt atcctaggta cgatattact tagtgtttac cggatagtcg
      961 tttacaaact gtacttaaga gtcagtcgtg ataagaaagt ccttatcgtg ggtttggaga
     1021 aagatgtcgc accagctatc caaaattttc aatctaagaa aaacaataaa cataaggttg
     1081 aagcagtggc aatcgataat tattacgaaa atacaaaaaa attgatcgat gatatggata
     1141 tcgtctattt ggcttctaaa atcccagaag atgaaaaaat caacatttat caattagtga
     1201 ctaaaaaaga gaagaaatta tttttaaata cgacttttga gaatctgaca atgataaatc
     1261 cgaatattat gaattttgaa gatgaaagca tcattgaagc ttctgggttc agaatccaac
     1321 cagaatacga actattcaag cgtctctttg atatcatcgt ttcgcttatt ttactgatcc
     1381 tcgcctcacc ttttatgcta atcacagcga ttttggtaaa aacaacttca cctggaccaa
     1441 tcatctataa acaagtacgt attacgaaaa atcaaaagga attttcaatc tataaattcc
     1501 gttccatgag tgcgacagct gaagcaaaat caggaccagt cttggctaaa agtaacgatg
     1561 cacgtgtgac accagtaggt aaatttatcc gtgctgtacg ttttgacgaa ttacctcaaa
     1621 tctttaatgt attgaaaggc gatatgtcga ttgtcggtcc aaggccagaa agaccatttt
     1681 tcgttgatca gttcaatgaa gaaaatcctt attattactt aagacacaat gtacgtgcgg
     1741 gaattaccgg atatgcacaa gtatacggaa aatatgtatc agattataac agtaaactac
     1801 gttttgattt gttgtatatc aaaaagtatt cct
//
LOCUS       DAXWLG010000272         1807 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXWLG010000272 DAXWLG010000000
VERSION     DAXWLG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.280"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..466
                     /locus_tag="ACGE4T_002750"
     CDS             <1..466
                     /locus_tag="ACGE4T_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8387950.1"
                     /translation="QLEESRQTKVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASA
                     ANPTELVYGDTVVEEEEFERVPNNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGE
                     IAIVEIDGDGVTCKEVYKDYESQTIILHSINELYEDRVVSPEQIRIIGKVVF"
     gene            543..752
                     /locus_tag="ACGE4T_002751"
     CDS             543..752
                     /locus_tag="ACGE4T_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFF8387951.1"
                     /translation="MPNYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESLN"
     gene            859..1512
                     /locus_tag="ACGE4T_002752"
     CDS             859..1512
                     /locus_tag="ACGE4T_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387952.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSLTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
BASE COUNT          686 a          316 c          343 g          462 t
ORIGIN      
        1 ccaattggaa gagtctcgcc aaacaaaagt ctacaacttt gctgaacagc aactcgaaga
       61 gcaaaatcga ggaaacgtgg tgcactttcc taaaaaggaa aaacttccga caatcaaaaa
      121 ttcagcttct gccgccaacc cgactgaatt agtttatggc gatacagttg tagaggaaga
      181 agaatttgaa agagttccaa acaatgctga ttttgctgtt cctattatag gcgattcaat
      241 ggaaccagtt atcaggaatg gacaatttgt atttgttaag gaacagccag acgtggaaga
      301 cggtgagatt gctatcgttg aaatagatgg tgacggcgtg acgtgtaaag aagtatataa
      361 ggattatgag agtcaaacga ttattctaca ctctataaac gaattatatg aagatagagt
      421 cgtctcaccg gaacagatta gaataatcgg gaaagtcgta ttctaaaaaa ataccccagt
      481 cgaagttggc gctccggcta ggggttagta tttattatta tagtagaaag aaggaaaaga
      541 atatgccaaa ttatgttgta ttgcaagttg tattaaaaga aaagtttata ggaaaagggt
      601 cgcaaaacct atcagaactt gaaaacacta taaatagaca gtgttctaaa ggttatcgat
      661 tacacactat ttccactaca aacggtggta gtaaaggttt cggtggtggc gatagaatcc
      721 aagctacttt agttttcgaa agtctaaatt aacaaaaaac acgccctctc ccctaccaag
      781 tttgagtgac gtgagatatt agacctatat agtaggcttc tttacaactt attgtatcaa
      841 agaaaagagg agtataaaat gaaaaaagtt agcgttatgt tgttgttaag tactgctctg
      901 ctactttcag cttgttcaaa taataaaaaa gctgaatcaa cagatgccac ttctaaccaa
      961 gaaacaaaaa taagtaaaac aaaagaaaca actgaaacca gttcatctac tagcaaatct
     1021 acatctaaaa cagattctag tttaacagtt acaagctcca accaagttac ggcggagcct
     1081 agcccaacag ttataagctc cagtcagagt acaatccaaa ccgcacctca agaagaaaca
     1141 tatgaacaga tgaaacaacg cactttacag tcaactccag ctgatcgtgc aaattggtcc
     1201 aacaaagagt gggaagcttt cggcgtggcc ctttatgaaa atggattgac tacagatgat
     1261 gctggcaata ttatcagtca agatcagaaa gaacaacaag cagcatctca acaaaatcca
     1321 gaagaccaac aaacaagcgc tcagcaagac gctgacactt tatcacttac tgattttgtt
     1381 aacaaatacg ggatgtcgcc tgttgcatgg aaagtacaga atggaatgtc tgaagaagaa
     1441 gcattgcgta caacacagca aaagacttcc ggtgaagttc aattaggatt ttctaaatac
     1501 ggaattcaat aatatatttt tatgccctac tattttgcct ataatctcta aaaaagttat
     1561 aaagaaaaaa gcccgtgctg caacacggac taaaatctcg tttctaagat cctacatata
     1621 aataatatca taggaatgag gagtaaaaaa gttattagag aaagaaaata taaaaagaaa
     1681 caaaggaaag agtaaaataa tgaaaaagaa aaaaggtaca caattatccg ttgattcacc
     1741 aaactggtta ttaattatta cagttttatg ttttgcagct tttataatgg tatcacccca
     1801 agtatat
//
LOCUS       DAXWLG010000273         1803 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXWLG010000273 DAXWLG010000000
VERSION     DAXWLG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.281"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..794
                     /locus_tag="ACGE4T_002753"
     CDS             <1..794
                     /locus_tag="ACGE4T_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFF8387953.1"
                     /translation="ETILRELKKEFSQNKFSYFLDLLIDAGLIKREERRYHLNFPIFD
                     PKDYLQQATSAAETIAEQLKRLSVDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRET
                     ELLRTTAGNEKYRFITLSSIEHFPLTLANYFFVQKNQLPVTKAFKELAELIGDVNESY
                     FFDQIEVIVDRIRKNKYKNRRPSIFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELP
                     TLDPSLTMEEMAFLKRQIFSELSKKFIPHAFSYIKEYGTVLVSKT"
     gene            928..1053
                     /locus_tag="ACGE4T_002754"
     CDS             928..1053
                     /locus_tag="ACGE4T_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFF8387954.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1187..>1803)
                     /locus_tag="ACGE4T_002755"
     CDS             complement(1187..>1803)
                     /locus_tag="ACGE4T_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387955.1"
                     /translation="TTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLDHSD
                     ITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFY
                     SFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVL
                     ALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT          692 a          267 c          346 g          498 t
ORIGIN      
        1 aagaaacgat tctaagagaa ttgaaaaaag aattttcgca aaataaattt tcctattttc
       61 ttgatttatt gatcgatgca ggattaatca agagggaaga acgaagatat catttgaact
      121 ttccaatatt tgatcctaaa gattacttgc aacaagccac ttcggctgct gaaaccatag
      181 ctgaacaatt gaagagactc tctgttgatg aacaaaaatt agcgatggga gaagttattt
      241 gggcgtattg tttcgaagat gaacggaaag aagcgtattt ttatggagta agaaacagcc
      301 gagagacaga attgctgagg acaactgcag gaaatgagaa gtaccgtttt atcacacttt
      361 cctctataga acacttcccg cttactttgg ctaattactt ttttgtacag aagaatcagc
      421 ttcctgtaac gaaggcattc aaagaattag ccgaacttat tggagacgta aatgaatcgt
      481 acttttttga tcaaatagaa gttatcgttg acaggatacg taagaataaa tataaaaatc
      541 gccggcctag tattttccat cagtctctac ttgtgacgaa tactataaag gaagaagaat
      601 cattcacatt agaattacct attgtcgaaa aaaacaattt tgagattgaa ttaccaacat
      661 tggatccttc attgacaatg gaggaaatgg cctttcttaa acgacaaatc ttttctgagc
      721 tgagcaagaa atttataccg catgcatttt cttatatcaa agaatatgga acagttttag
      781 tgagtaaaac ctaagagagc agtaagcaaa aattatactg actcttgcag gaggagcctt
      841 tctcttcctt tatggttaaa agaattattt gagtattttg cctatctacc acctattaga
      901 ttcactacat ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca
      961 aaggaagtag tgataagaaa agttagattt ttaaataatc aatattatga ttcagtcaag
     1021 tatggcattt tatgggaaga actagctgat tagattagga agtccatttg ataatccaga
     1081 atcactaaga aacgaatggc gagtcattaa taatttatcc aatcttgggt tattctttta
     1141 gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat
     1201 gctttttatt ttgtttgttt tttctttgcc acataaatac acctagactg acgatgaaga
     1261 gcatatccaa cagatttcct gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag
     1321 caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt gacaggaact aatcgaaaga
     1381 agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta aaaatggata
     1441 aaatcacgac taaaccattc atgataaaga agaaaagcgt attgtgatta aacgaataaa
     1501 aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta
     1561 acgtaccagg atgtcgatga aaaaaatagg caccgaaata aacagaaaac agtaagccgc
     1621 aaagagaaga caaaatcaaa aaaaacggat tttcttcggc ttctgtgata tcagaatgat
     1681 ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga ggtcttaagg tatgaataac
     1741 atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg
     1801 ttc
//
LOCUS       DAXWLG010000274         1799 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXWLG010000274 DAXWLG010000000
VERSION     DAXWLG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.282"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            123..521
                     /locus_tag="ACGE4T_002756"
     CDS             123..521
                     /locus_tag="ACGE4T_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8387956.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            505..1199
                     /locus_tag="ACGE4T_002757"
                     /pseudo
     CDS             505..1199
                     /locus_tag="ACGE4T_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          683 a          260 c          347 g          509 t
ORIGIN      
        1 gagaaatcac acaatatcga aacaaattcc gttaggaatg tgttattcaa taaattttat
       61 aatctagaaa cattttgaat aaaaactatt agacaaatga tattttacga ggaggaacag
      121 agatgacgat ctttgaaacg gttgtaacag atgttggtat agaagcagaa gtattcagga
      181 aagaaaaaat aatgatttta tttggagatg aagcaccgga aacattaaca gattattgtt
      241 ataagattga attgaatcag gtaagaaaag aaataacttg tgatatgaag ctctgttttg
      301 ataaacaaat gtatgtaatc acagcagtag gagatgttgt ccaaactaat ttgaatgaat
      361 taggacatat cacgataaaa tttgatggat ctcatgagcc ggaacttcca ggaacaatgt
      421 atgtagaaga taaggaaatg ccgcatttaa gaaaggggac aatcattaaa atcgatcaag
      481 aggatcagaa tgggggaaag ctatatggaa atcatgcttg atacagcaaa tacaaaaagt
      541 atttattctt gtcaacagtt tttgaattta acaggcgtaa caacaaatcc tacaattctg
      601 aaaaaagaag gagcgtcaaa cttcttctcg catatgaaag aaatcaaaaa aataatcgga
      661 cagattccta tccatattca gacaatagga agaacggaag aagaaatgat agaggatgct
      721 cgagttatca tagaagaact gggaaaagat acgtttatca aggtgccggt gaatcaagca
      781 ggccttcaag cgatcaaaaa gttgaaaaaa gaaggttatt tgattaccgg tacagctatt
      841 tacacagaat tacaagggta tttagctatt aataatggag cagattatat tgcaccttat
      901 tataacagga tgcaaaatca aggcattgat gctatgaaag tgattgattc gttgcggact
      961 gagatcgaac gtacgcaaag accaacaaaa atattagcag ctagtttcaa aaattgtgaa
     1021 caagtcacta cagccattcg atctggagct cagcgataac gttaggtaaa gagctactag
     1081 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1141 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1201 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1261 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1321 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1381 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1441 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1501 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1561 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1621 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1681 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1741 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttg
//
LOCUS       DAXWLG010000275         1795 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXWLG010000275 DAXWLG010000000
VERSION     DAXWLG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.283"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>1795
                     /locus_tag="ACGE4T_002758"
     CDS             <1..>1795
                     /locus_tag="ACGE4T_002758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387957.1"
                     /translation="TQEVDKDATVLSDTPRDLVNLEDTSTDIENVTPQKIGKKDSTSL
                     SEENLSSPASSILRRRTRSLDVQTGETFLEDWALPDNSNISGSLITQTGITYVGVKTD
                     DPTQLHLKMAYRAAATPAAIDWIYIRVDPELTKYIEEINIKNTVNIGAQVGYQRYFEK
                     VDANTLSDVNTFGTPTGVSDAYAQVDQTTGGIYRVLLDKFFTRIPGVKPKERGALSGN
                     WGVFPSVPLEAERYEANIYIKLSKDVAELQQELGRDKFGVQLRLQGQSSSGVATIAKE
                     SLNKNTIIDLSKQESIENNSNWLNSPSVHTITTTYEGKQMGSDGQFSNTLEGTDKSLI
                     RVQNTIMNNLLYGTALDLGRPVSFNIEIDPQILNLVSDDDYITIWQVYRENSINHHDG
                     GSNAPVMLKKNMFVDNKIKIEVNKDKAGQYENGVYYVNAVESFMNTVSSSPFVGANFI
                     DIPVMPKPLIEDEGQLVSAEIKTWFTDKKNDQERVVRNSLASSYINLFPTTINFYDYS
                     TKDTEQLLKLRESTYLVSTFGQEINGMDMGETYEISNYEFVTADPESLIITQKDGNVI
                     NLYYKKLSSDAEFYEPEVEKEEVNYGGTIDLT"
BASE COUNT          649 a          253 c          352 g          541 t
ORIGIN      
        1 atactcaaga agtagataaa gatgcgacag ttttatctga tacaccaaga gatttagtta
       61 atttagagga cactagtaca gatatagaaa atgttactcc acaaaaaata ggtaaaaaag
      121 acagtacgtc tttatcagag gaaaatcttt cgtcaccagc atcaagtatt ttacgcagaa
      181 gaactaggag tttggatgtt caaactggag aaactttttt agaagattgg gcactaccag
      241 acaattctaa catttctgga agtttgatta ctcagactgg aattacttat gtgggtgtaa
      301 aaactgatga tcccactcaa ttacatttga aaatggctta cagagctgca gcaacaccag
      361 ctgcaattga ttggatttat attcgtgtgg atccagagct tacgaaatat attgaagaaa
      421 ttaacataaa aaatactgta aacatcggcg cgcaagttgg ttatcaacga tattttgaaa
      481 aagtagatgc taatacattg agtgatgtaa atactttcgg aaccccgacc ggtgtaagtg
      541 atgcttatgc acaagtagat caaacaactg gcggaatcta tcgtgtgtta ttagataaat
      601 tttttacacg tattccaggt gtaaaaccta aggagcgagg tgcgttatct ggaaactggg
      661 gtgtatttcc atcagttcct ctcgaggctg agagatatga agcaaatatc tatataaaat
      721 tatcaaaaga tgtcgcagag ttacagcaag agcttggaag ggataagttt ggtgttcaac
      781 ttcgtttaca aggtcaatca agttctggtg ttgcaactat tgctaaagaa agtttaaata
      841 aaaatactat tattgatcta tcaaaacagg agtcaattga aaataattca aattggttaa
      901 actcaccatc tgtgcataca attactacta cttatgaagg aaagcaaatg gggagcgatg
      961 gtcagttcag taatactctc gaaggaactg ataaaagtct gattagggtt cagaatacaa
     1021 tcatgaataa tcttttatat ggaacggcac ttgatttagg tcgacctgtt tcatttaata
     1081 ttgagattga tccacaaata ttaaatttag tttcagatga tgattatatt acgatttggc
     1141 aagtatacag agaaaatagt atcaaccatc atgacggtgg atcaaatgca cctgtcatgt
     1201 tgaaaaagaa tatgtttgta gataataaaa ttaaaattga agtcaacaaa gataaagcgg
     1261 gtcagtatga aaacggagtt tactatgtga atgcggttga aagttttatg aatactgtga
     1321 gttcctctcc atttgttggt gcaaacttta ttgatattcc tgttatgcct aaacctttaa
     1381 ttgaagatga aggtcaattg gtatcagcag aaattaaaac atggtttacg gataaaaaaa
     1441 atgatcaaga gagggtagta agaaactcac ttgcttcaag ttatattaat ttattcccta
     1501 ctactattaa tttctatgat tattcaacca aagatacaga acaattattg aagttacgtg
     1561 aatcaaccta tttagtttct acgtttggtc aagaaattaa tggaatggat atgggggaaa
     1621 catacgaaat tagtaattat gagtttgtga ctgccgatcc tgaaagttta ataattacac
     1681 aaaaagatgg aaatgtcatt aatttatatt ataagaaact ttctagtgat gctgaatttt
     1741 atgaaccaga agtagaaaaa gaagaagtca actatggagg aacgattgat ctgac
//
LOCUS       DAXWLG010000276         1780 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXWLG010000276 DAXWLG010000000
VERSION     DAXWLG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.284"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            19..828
                     /locus_tag="ACGE4T_002759"
     CDS             19..828
                     /locus_tag="ACGE4T_002759"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8387958.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFSLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            868..1365
                     /locus_tag="ACGE4T_002760"
     CDS             868..1365
                     /locus_tag="ACGE4T_002760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFF8387959.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          616 a          322 c          340 g          502 t
ORIGIN      
        1 agaaagtagg aagataaaat gaaactagca gcgattgatt tagacggtac cttgcttgat
       61 cgaaatggaa agataccaga agaaaatatc caagcacttc ggaaatttga tgaaaatggc
      121 ggaatcgtaa ccgtcgctac cggaaggaac agcatttctg ctaaagatat tttttccgaa
      181 ttaggaatcg gtggctattt gatttcttcc aatggttcac tgattgctga aatgaaggat
      241 ggaaaaatgg cgaaagttct cagacgttca aaaatagata gcgatacact gagaaaagcc
      301 ttcttctatg ctcaagaagc gaaaatatcc atcattgcta gccgagaaac ccaagacgat
      361 cagctgacat tcgatgaatc aaatctagtc aaagatgacc cttattatga acatttcagt
      421 ttacagaacc attcttttga tcaaatttta aatatgttgg aagatccttc tcttagctat
      481 cttaaattag cattaacaga taagagtgaa gaaaaactac agaagctaca aagagatctg
      541 caagcagatg gtatccaatc agtattctct gatccgcatt tcttagaaat cacgcctaca
      601 ggtatcacta aagctcattc acttgttttt ctggcggatt accttggaat cgaatcgagt
      661 gacatcatgg cttttggcga tcaagaaaac gacattgcta tgttgaatta cagcgggttg
      721 agtattgcga tggggaatgc acagcgacaa gtaaaagaaa ttgcaaacca agtaacactg
      781 acgaatgaag aagctggtgt tgcttatatt ttaaaaaact tctattaaaa agaataaaat
      841 gacgatatga atgtaggagg aaagaaaatg ttagttatta ttgatatgca aaatcatatt
      901 cttgatccaa acagcgaatt ttatattcca gagtcagatc aattggcaga acgtattgct
      961 caaagattag ctaaagcacg ccaaaataat gaatatattc tcttcacacg ggatatacca
     1021 gttgaactga aggacaaaga agaagaacga gaagatttgc aattgatccc tcttctttct
     1081 ccacaagaaa atgaacgagt gattaaaaag tattacttta cgattccacc tgaaacttta
     1141 gctgaaatcc aggaaacttt atttgaaagc aaagaagaac aaaaagagat cgaagtagtc
     1201 gggattgaaa caaacttgtg tgtactctct aatctgatag gcctgcagag tgcttttcct
     1261 gaagcagact tttttgttga tccaatgtta gtctccagta gaaaacacgg agaatccgct
     1321 ttagagctgt tgaaagattt caacgtctct atcgtcgaat cataaaaata tctatcttca
     1381 tttacgatat ttaacacaca ataaaaaaag cacgcagaac tgttttagtc ctacgtgctt
     1441 tttctatcaa agtagattgg cttttttatg acacagagtg aaacggcagg ctcacgacct
     1501 ctttcttaaa tggtgttcaa tcagctgacc ctcggtatta gcttaacaaa cggcaaaata
     1561 tgaggaacta tttttccgtt tgttaagcta atactcaggt caaaacgctg atttcacaac
     1621 ctcttcctta aacggtattc aaaagcttgc atctgcagta ataagaacag ccaagtaaaa
     1681 acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca gctttttcac
     1741 aacctcttat gtaccttgta ggactcgaac ctacgaccgg
//
LOCUS       DAXWLG010000277         1779 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXWLG010000277 DAXWLG010000000
VERSION     DAXWLG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.285"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..768)
                     /locus_tag="ACGE4T_002761"
     CDS             complement(<1..768)
                     /locus_tag="ACGE4T_002761"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFF8387960.1"
                     /translation="MEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQNADYYA
                     YSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSALLYVIR
                     LFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFKYIFFIL
                     VAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKYDAYFDR
                     NSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISL"
     gene            complement(758..1654)
                     /locus_tag="ACGE4T_002762"
     CDS             complement(758..1654)
                     /locus_tag="ACGE4T_002762"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8387961.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
BASE COUNT          621 a          302 c          223 g          633 t
ORIGIN      
        1 taagctgata actttttttt cgtaaatatt catttttgat aatttatgaa ctaaaaaaag
       61 atttgctgaa aaaaagagag caaacatcac tacgtgagaa atcgatgtac tgtttctatc
      121 aaaatatgca tcatacttcg tatttataaa tattgaactg atataacttt gtccaataga
      181 gaataggagc aagctaagga ttgtaacata tccagtaatc tttttattaa tttttacaat
      241 aaaaaaaggg agataaaaat atgcaagatt atggaacagt ccagccacta agataaaaaa
      301 aatatactta aatatagctt tgctgcctcg caatttagtt agatgataaa aagctagtaa
      361 tatgaatatc aatgcgctaa acatacgtac ttgtattgct tgtgaagtaa aaccaaaaat
      421 aaaaaacaag aacaaaacta aaaaaacatt agttgtgaat aatctgatga catacaataa
      481 agctgagagt aagacagtag aaataatcaa taaaaaaatc tgatactgac tggaaataaa
      541 ggtacatacg tacataacag ctctgaatcc cggttcaata ctatctgaac taaagtccat
      601 accaaattgt gctatcccat tatacaagac agaatatgca taataatcag cgttttgtgt
      661 gttaaatgcc atgatgatcc acattatcaa aagtagcacc ccagacaaaa tcttgttcga
      721 tgtactccat tttttttgtg aaacactctt ttcttgatta atctccaatg acgcctctgt
      781 atccatctcg aacccccctt atagccgctc taaatctact gagcttatca gtctcaaaag
      841 ctattgcaat aaaaaatttc aataatcttt tattcataag gcgggaaaat tccttatcac
      901 ttttatacat tcttttcacc aatgttgaat ttcttgcaat aaaatacttt cgtgttgaat
      961 tatgattgtc tattttaatt ttttttgatc ctatttttaa attttctcca tctccaacgt
     1021 gatgataaaa atagacttga tttgttacat aaatgttata gccattattt gcaacccttt
     1081 tacaaaattc aacatcaatc tgatcaataa aaaattcatc cttcatattt ccaacatcgt
     1141 caattaattc acttttgaaa aacattccag aggaatttgc aaatgctatt ttcatggaac
     1201 cactagttga agcaaagtgt ttttttacgt atctccctct ctcatttttg caggtaaaag
     1261 agacagattc atcattgcta ttctcaaagt aattgggaac aataatccca atgtccttat
     1321 ctttcgcttc aatattggta aactcttcta ataattttaa agaaagattc ttttgtatga
     1381 tggtatcttg atctagtaaa aggatataat cacatttctc ttttttggca atttcaatac
     1441 ctatgttttg agctgtcgca agcccaatgt tctcattcat tttttttaaa attaagtttt
     1501 ccatcatttc aaattgaaaa gagttaatat tatctgagcc gttatccacg ataataagtt
     1561 tgttctgttt ctcttgtatt aacgtatcca ctaataactt tatgttatta tctggattgt
     1621 aggtaacaat aattatccca attttatttt tcatcgttca tcaccttgta cacttcattc
     1681 catttcattt tcaatacttg ctcagagaat atagtggcgt aaatttttct aacattttca
     1741 tttatgtttt tattacactg ataaaatttc aacatctca
//
LOCUS       DAXWLG010000278         1753 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXWLG010000278 DAXWLG010000000
VERSION     DAXWLG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.286"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            128..958
                     /locus_tag="ACGE4T_002763"
     CDS             128..958
                     /locus_tag="ACGE4T_002763"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8387962.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            930..>1753
                     /locus_tag="ACGE4T_002764"
     CDS             930..>1753
                     /locus_tag="ACGE4T_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8387963.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQ"
BASE COUNT          641 a          220 c          359 g          533 t
ORIGIN      
        1 gataagatgt cctcacaagg actagatgaa gatgaaaata tcagttcatt agcagaaagt
       61 gcatttccaa aagaaaaaac gtatcgttag attggaaaaa ttttgagcaa agaagtagga
      121 gagaacattg gaaaataaaa atataagtat actgctttct ttgtatgata aagaaaagcc
      181 ggaatattta aaggaagcat tagctagtat ctttgagcaa acggtgatgc cggcagaaat
      241 cgtattggtt tatgatggcc ctataaatga gaagcttcag gcagttgtca cttatttcca
      301 gcaaaaagtt cctcacttgt ttacagttgt aaaattagaa aaaaatcaag gattggggat
      361 tgctttagca actggattag aacatactaa aaatcgctta gtggccagaa tggatacaga
      421 tgacatcatg gaaccacaca gattggaaaa acaattagag atcttccaag aatatccaga
      481 aatagcgatt gttgggagta atattgaaga atttactggc acacttagca atgttatcgg
      541 aagaaggatt gtacctgagt ataatgatga aatccgtgtg ttttcgaaaa gaagaaaccc
      601 ttttaatcat atgacagtca tgtttgataa aaatgctatt ttagaagtgg gaaactatcg
      661 cccattaatt ggatttgaag attattattt gtgggtaaga ctattgaagg cgggatataa
      721 aggatataat atccaagaga ttctagtaca tgctagagca ggtgctgaca tgtatgctag
      781 acgtggagga agtaaatatc ttttaccagg aattaaaggc cgatatcgcg tatggaaaga
      841 tggtctagga tcactaaaag attttctgat agttatttgc ggacatcttg taattagttt
      901 attaccgaat aaaattaggg gaaaagtata tgaatcaaaa cttagaaaaa caaactaaac
      961 tttctataat catacctttt tacaatgcaa agaatacttt aagaagagct gtagacagcg
     1021 tcttaacgca aggttatccc aatattgaaa tcttgttaat tgatgatgga tctacagatg
     1081 gtagtagtga cattggtgac gaaattgcta atctagagag taaagtagca tgttatcact
     1141 tggaaaacgc tggtccatca gttgctagga actatgggat aaaagaagca actggaaatt
     1201 tggtaggatt tttagatagt gacgattatt ttttacctga ttgttttaat aatgttttga
     1261 aagactacca taggaatcac cccgatatca ttgcttttgg attaagtaaa ggaaaaaata
     1321 tagaaaatgc aaatttattt gttcccgaaa agaagattgg ttgttcaagt gaagaagcaa
     1381 ttgagcaaat gtttagaagt aaggcagtgg acttttacgt atggaataaa ttttttaaaa
     1441 gggaattatt caatcatata gtttttccag aaaataaatt atatgaggat atggttccta
     1501 tgtatgaagc ttttagggcg gctagtaaaa ttgatatttt gccattttca ggtatttttt
     1561 attatcaaaa taacgatagc atcgtttatc agtcttttaa ttcaaaacag tatgataata
     1621 ttttgcagag aaaaatatta gttgatgaag taaagaaaga ttatcctcga cttattaatt
     1681 tggccaatgc tcggttagta gacggatatc tttcaacagg gtttaaaatt actggcaaaa
     1741 atcaaaatca gaa
//
LOCUS       DAXWLG010000279         1750 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXWLG010000279 DAXWLG010000000
VERSION     DAXWLG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.287"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..770
                     /locus_tag="ACGE4T_002765"
     CDS             <1..770
                     /locus_tag="ACGE4T_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8387964.1"
                     /translation="PLYIWKTKNKDGTDRCGPTCINKICGYREMVTKNQKEAIQKANE
                     AMKKDAINRMLNSSMITDDAIWTFDFDGYKVVDQETAQIKAMAQEWAKKIVNGSTIHA
                     VITGRTGAGKTHLGAAVIKEVMRASNYKIACSFISYRELLEQLKFAMNDPEARKAVTG
                     SLMAEIKKSDFVVIDDLGAELGRMEENNQATPYDVDVLTSLTEARLNKATIFTTNLSS
                     KQLKHAYGERVFSRVMNGTKGNIAVFKTTTDKRRNPV"
     gene            767..1009
                     /locus_tag="ACGE4T_002766"
     CDS             767..1009
                     /locus_tag="ACGE4T_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387965.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            1152..1520
                     /locus_tag="ACGE4T_002767"
     CDS             1152..1520
                     /locus_tag="ACGE4T_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFF8387966.1"
                     /translation="MATRSKYGNKKHEVDGITFDSIAEAEYYDLALWQAEANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHK
                     YQVPLILAKKYRNTFKEERF"
     gene            1535..1696
                     /locus_tag="ACGE4T_002768"
     CDS             1535..1696
                     /locus_tag="ACGE4T_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8387967.1"
                     /translation="MTTEEVIQMHIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
BASE COUNT          655 a          282 c          410 g          403 t
ORIGIN      
        1 atccattgta tatctggaaa acaaaaaata aagatggtac tgatcgatgc ggtcctacat
       61 gtatcaataa gatttgtggt taccgagaaa tggtaactaa aaatcaaaaa gaagctatcc
      121 aaaaagcgaa tgaagcaatg aaaaaggacg ctatcaatcg aatgcttaac agttcaatga
      181 ttacagatga tgccatatgg accttcgatt ttgatggata caaagtagtt gatcaggaaa
      241 cagcacaaat aaaagcgatg gctcaagaat gggccaaaaa aattgtaaat ggtagcacga
      301 tccatgcagt gataaccggt agaacaggag ctggaaaaac tcatctaggc gctgcagtga
      361 ttaaagaagt catgagggca tctaattata aaattgcttg ttcatttatc agttatcgag
      421 aacttttaga acagttgaaa tttgcaatga atgatccaga agcaagaaaa gccgtaacag
      481 gttcgctgat ggctgagata aagaaatcag atttcgtggt aatcgacgat ttgggtgcag
      541 aactaggaag aatggaagaa aacaatcagg caacgcctta tgatgttgat gttctcacat
      601 cgctcacaga agctcgtcta aacaaagcta cgatattcac aaccaattta tcatcgaagc
      661 aattaaaaca cgcgtacggt gagcgagtgt tctctcgtgt aatgaatgga acgaaaggga
      721 acatagctgt attcaaaaca acgacagaca aaaggaggaa tccagtttga cctttgtagt
      781 gaaaaaaatg tgctacttag atagccgagg gcgaggagaa gctagtgtcg agtttgctaa
      841 acaccacacg actaaagaag aggctgacct tgttgcaagt gtttgtggcg gcgaagtagt
      901 cgaagttatt aaacccaaaa gacgattttc aaaacctaag acaaagccag tgaaaaaaga
      961 gtgttgttgt ccgaaaagta accaagcatg gatgagaggt gcaaaatgac ttgtttaaaa
     1021 tgcaacgatg aaacagttat ttggtataag acatcgcttg gatggtcaac ttgtgaacct
     1081 tgtccaatat gcaatgaaaa tggacgacgt tcgaaagaac gactcgaaag actaaagaag
     1141 gagtatagca aatggcaaca agaagcaaat acggaaacaa aaagcacgaa gtagacggca
     1201 tcacttttga ttctatcgca gaagcagagt actacgatct agccttgtgg caagcggaag
     1261 cgaatggctg gaaagtgaaa cttcaggaac gatttgagct gatgccgaaa tttgaactag
     1321 aaggaaagaa gtatcgcaag atcgagtata ttcctgactt cacattttat aaaaacggca
     1381 aacttgtcaa agtcgtagat gtcaaaggaa tgcagacaaa agactttaag atcaaggcaa
     1441 agttgttttg tcataaatat caagttccgt tgattttagc taaaaaatat cggaatacgt
     1501 tcaaggaaga gcgtttttaa cgaggtggtc catcatgaca acagaagaag tgattcaaat
     1561 gcatattcga agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt
     1621 caatgaaacg gcgagaaagg caatcgattt gcacatagaa aatttaagaa gggaaattcg
     1681 tagattggag gaatgagcgt ggataagaaa gcagcaatga aacgaatcat tgaactgaca
     1741 cattctgaga
//
LOCUS       DAXWLG010000280         1731 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXWLG010000280 DAXWLG010000000
VERSION     DAXWLG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.288"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..1084)
                     /locus_tag="ACGE4T_002769"
     CDS             complement(<1..1084)
                     /locus_tag="ACGE4T_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFF8387968.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQK"
     gene            complement(1194..>1731)
                     /locus_tag="ACGE4T_002770"
     CDS             complement(1194..>1731)
                     /locus_tag="ACGE4T_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFF8387969.1"
                     /translation="ATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISY
                     NYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVAS
                     WALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVAS
                     LSMIGVLIGRKVLKKYKK"
BASE COUNT          634 a          325 c          239 g          533 t
ORIGIN      
        1 ttttttgttc taaaactaag ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc
       61 tgacattttc aaatgcaatg tcacaatttt taatctgtgt ctcgttccca tattttagtt
      121 ggtcttgctt catttctaaa taaagggagt ctagtgtttc gactgcatat cttgcttcaa
      181 acaagtgcat acttacatac attattctca tgaaagaaac aaaaataatt ccacttaaaa
      241 aaaacaacat caacaagtca acagctaata tcttgggagt ggtaagagaa gttataaaaa
      301 aggttatagg aactatcaaa atgcaaacta tgccaaaaaa tagccattga aaaagaacaa
      361 aaggtgtttt cccactcaaa gaatacttat atgcaaactt agcataatcg ttaattgctt
      421 tattcaaggc tttaaatgat gttactttag caccaaatat ctttataact tgaattccgc
      481 ggatatattc taccgtttca gagcttaaca catctaacga ttcttgatat ttttgaataa
      541 aatttgtatt ctctgtcatt ttctttaaca agaatacacc aattactaac atggcgatta
      601 gaaccaatcc aattttaaag ttaatgaaaa agcctaaggc aactgataaa acaggtgtta
      661 ctatggcttg agcactatct ggaatcaagt gagcaattgc catatgtgtt ttagccgcgt
      721 tgtcatcaat tgtctttcga atatatcccg aagaatttaa atcaaaaaaa cgaaaacttg
      781 cttctgttaa accgctaatt cccttttttc tcaaattagt ctctaagcga aacccaagga
      841 tatgagaaaa taagccagaa aaaatataaa ataaagctcc taaagttaac caagcaacag
      901 tttgaaccgc ataactctta gctaaatatt catctcctac aataattaga gactgaagaa
      961 atttgtaaat actgtaatat ccatagactg ttaatacgac agaaaaatca gaaaataaag
     1021 tggctaacca tccgtagtat ttcattttag ggacataact aaataatttt ttgtatactt
     1081 tcattttttc ctcccccgca ttcaatttct tctcttattc gtttacaact gttaatgctt
     1141 tccgtaatag cgtaaattag agatgtacaa acagataaaa ggtaaactac gttctatttt
     1201 ttgtattttt ttaagacttt tcttccaatc aatacaccaa tcatagataa acttgctaca
     1261 actaacactg aaatgattcc ccatatacct accgcatagt ggatcattgc atccattgtt
     1321 gcagcagatt gtcctttatc tatccaatct tgacgaaaac gatctgtaaa gaaccatata
     1381 ggaatccact gtccggcagc actgcctaat tgcattagag cccaagacgc aacttgagga
     1441 attgctcgat catatccaaa tacagataga ataatatcag ctaagatcgc catactaatc
     1501 gaactgataa accaaggtaa gtacgccccg cccattaacg taaaaaataa caataaaaca
     1561 attgtgtaaa tagtaaaaat tccttttcta gggtagttat agctaatata aacaaatatg
     1621 attccagata ataatccaaa aatcccgggg gatatgctgt gaccaaacgc cccaccaata
     1681 ctactaacca ttattgaaaa aaagtaaatt actagggaaa taactgtagc t
//
LOCUS       DAXWLG010000281         1715 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXWLG010000281 DAXWLG010000000
VERSION     DAXWLG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.289"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(567..1592)
                     /locus_tag="ACGE4T_002771"
     CDS             complement(567..1592)
                     /locus_tag="ACGE4T_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8387970.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          530 a          274 c          329 g          582 t
ORIGIN      
        1 tattgtcaca acctcttttt tggtttgtct aaaataattt gtctaactag aagaaaagtg
       61 ggaaaggatc ttaacaacat cggttcgtat ccaattaagg tatcggaacg atcatcttcc
      121 aagagaagtc aatcaacaaa agtaaagata gatcattttt tgaatgcaaa ataaattgat
      181 gggaagatag agcggttatt tttaatattt tgctagctat tattgaataa ttcctcgaat
      241 aaaacaaaac cagtagacat gtctgttatt ctatttatgt tttagagata agtatcccca
      301 aataacatgg attgaaataa gggtaaatgt gtttaaaaaa acgaactata aaaacttata
      361 cacaacgtgg ttaaaaatag ttttttcttc cctgtggaaa aaatagttta acacaaacat
      421 tatagttatc cacagctatc taaaacagat ggaaaataag aaaaattcgc taaaataccg
      481 ggagggggta acgagggatg agtgattgaa aaaagaacag agtaactgac ttatcctcag
      541 tgtttctgtt cttttataat tctgttctac atttcttgtt gttggatcga atctgtgaga
      601 gtaatctcgg ttgaaagaat gattcttttt tcttcagggt attggtggtg gattagttgg
      661 aataaaagtt ctgtggcttt gattcctaac tgatttgtcg gaatgtctat ccgagtcaac
      721 tgtggcgaaa atagggagga caataaggga tcactgaatc ctgcgatcga aagatcatca
      781 ggtatctgct ttccagattg ttctgccact ttgtaaatac tcaatgattt taatgcatcg
      841 gtagaaaaaa tcgcagttgg tctttctcgt agcttcatta aatcattcat tgctgccatg
      901 ctatcttcta agcgaaagcc gccgtctata atccattcat tacggatatc taggtggtga
      961 tcaaataagc attgacggta accattgaga cgatcattgg atacataaac ttctgtcgga
     1021 gcatggagac agccgatatg acggtgtcca ttcttgatca gtgtttccac caaatcgtaa
     1081 ctatcctgga aattatccgt gtccactgag tagatgttcg tgtagtcgtt tccgactttt
     1141 ccgattacca caataggaat attgtattga tccaattcaa aaagaagtgt ttcatccatc
     1201 gtagagctta aaataataat tcctcggatc attttttctt ggatcttttt cgcgcatttt
     1261 tctagctctt cttctggtgt ttttgccgtt tgtagaatca agtcgaagtt attttgttca
     1321 gaaacggcag aaatggattg aataacatct gaaaaaaaag gattgccata ggtcgtatga
     1381 gaagtacgat gagataatgc tagaatcgcg tcaaaaccat ttcctgacag tcctcttgca
     1441 attttgctcg gttcataatt caattctttg attgcttgaa gtacacttgc ttttgcttta
     1501 tcagagatat ttggctggtt atttaatact ctggagacag ttgattttga gacattagct
     1561 tttttcgcaa tgtcataaat agtaggtttc aattttatat gcacctcctt tttgggagcg
     1621 ttttagaata attccatagt acaatagaca ttcttttttg tctatgattt tgaaaattta
     1681 ggaatagctt gcatgatcat gagagaccat atttt
//
LOCUS       DAXWLG010000282         1683 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXWLG010000282 DAXWLG010000000
VERSION     DAXWLG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.290"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            172..1314
                     /locus_tag="ACGE4T_002772"
     CDS             172..1314
                     /locus_tag="ACGE4T_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8387971.1"
                     /translation="MAKFEQYKKKNGDKAWKFQAYLGINPETGKPVKTTRRNFKTQRE
                     AKLALARLQSEYEDNLLTKEKPKTYKDVYDLWMTEYKRTVRGSTLLKTERIFKNHVLE
                     ELGDIYISEITPIKIQKLMDKWANKYDTAPKMMNYTGLVFKYAVRFGMIESNPTDAIR
                     KPKRRKKATVEEPFYDKKQLKLFLDELYNQPNLKIQAFFRLLAMTGMRKQEAGALEWR
                     DIDFKAKTVNIYKAVTRTANGLEIDTTKTVGSSRIISIDQGTLDKLLEWKKVALPPSD
                     EWLIFGHSSAKKPHDIMSLDTSRKWLLSIQDKMDKKQKKKLPRITVHGFRHTQASLLI
                     EMGASLKEVQFRLGHEDIQTTMNTYAHVSKLAKEQLADKFNKFIDF"
BASE COUNT          669 a          253 c          284 g          477 t
ORIGIN      
        1 ttatgtcatc tacaagtata aaaaaacaaa aataatcgaa atataaaaat tacaaaacat
       61 taatatgtta tgtgatgcac atccatttaa taacagcaga atattataat ggatgtgtaa
      121 aaatcactga gattttttag tcattattac cagaaaagtg agttgattca attggctaaa
      181 tttgaacaat ataagaaaaa gaacggggat aaagcttgga agttccaagc atatttagga
      241 atcaaccccg aaacaggaaa gcctgttaaa actactcggc gaaattttaa aactcaacgt
      301 gaagcaaaat tagcactcgc aagattgcaa agtgaatacg aggacaattt attaacaaaa
      361 gaaaaaccaa aaacatataa agacgtatat gatttatgga tgactgaata caaaagaaca
      421 gtacgaggat ctacattgtt aaaaacagaa agaattttta aaaatcatgt attagaagaa
      481 ctcggcgaca tatatatttc tgaaatcacg cctatcaaaa ttcaaaaatt aatggataaa
      541 tgggcaaata aatatgatac agctcctaaa atgatgaatt acacaggact agtttttaaa
      601 tacgccgttc gatttggaat gattgagtcc aatcctacag atgccatacg caaaccaaag
      661 agaaggaaaa aagcaactgt tgaagaacca ttctatgata aaaaacaatt gaaattgttt
      721 cttgatgaac tatataatca accaaaccta aagattcaag ctttttttag attactagct
      781 atgactggta tgcgaaaaca agaagcaggc gctcttgagt ggagagatat agatttcaag
      841 gctaaaacag tcaatatcta taaagccgtt actagaactg caaatggact agaaattgac
      901 acaactaaaa cggttgggtc tagccgaata atttcaatcg atcaaggtac tttagataag
      961 cttcttgaat ggaaaaaagt tgcccttcct ccatctgatg aatggctcat ttttggacat
     1021 tcaagtgcaa aaaaaccaca tgatataatg agtcttgata cctctcgaaa gtggcttttg
     1081 agtatacaag acaaaatgga caagaagcaa aagaaaaaac tacctagaat tactgtacat
     1141 ggatttagac atactcaagc aagcttgttg atcgaaatgg gagcatcgct taaagaagta
     1201 cagtttcgtt taggtcacga ggatattcaa actactatga acacgtacgc tcatgtatca
     1261 aaacttgcta aagaacaatt agcagataag ttcaataaat ttatagattt ctagctatgt
     1321 ggcattcaaa atggcattca attaaatttg agcattgata taatagtatt taagcaactc
     1381 ccgccgtctc catatatgta tggttgagac agataaacat gtatgaaaaa cgttgttaaa
     1441 tcaacgtttt tttattttgg tcaaggagag aaaagtagag aaatacaaaa ttttttgcat
     1501 tgattttgca ttcaaaaaaa cattcaaaca tttagaaatc cccaatacgt attcaggaaa
     1561 atctatttta aggtttacaa aaagaacttt tgttcgtaca ctctttttga ggtgaacttt
     1621 tatgctgatg gaaggaaaaa cacaattatg gtttaagcat gttccttcaa ataagttttt
     1681 tta
//
LOCUS       DAXWLG010000283         1678 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXWLG010000283 DAXWLG010000000
VERSION     DAXWLG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.291"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            233..>1678
                     /locus_tag="ACGE4T_002773"
     CDS             233..>1678
                     /locus_tag="ACGE4T_002773"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8387972.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEEE"
BASE COUNT          571 a          317 c          329 g          461 t
ORIGIN      
        1 actatttaac aaggttattc gaaccttatt ttaagttact tattatttaa tgtatcgaaa
       61 aaggtgaaat aggtttgtta taataagaat taagtaagac atgaattgtc taagcaaatg
      121 tagcttatcc atttcattaa aatacatatg gtgaaaaaac aagttcaaat agtaactaat
      181 tttttaaaaa actgatttat aaaaaataca acacgaatga ggaaaataac caatgacaga
      241 acaaatcgaa aaaaattcaa aaagcttata tcaggcactg tggaattccg cagatatcct
      301 gcgttcaaaa atggacgcta atgaatacaa gagctattta ctaggcttgg tcttttataa
      361 atacttatcc gacaacatgc tgcggtatgt ctccgttcta ttggaggaag aaacggacga
      421 cctaaataaa gcacaagacc tttacgttga agcatttaaa gatgcagata taaaggatga
      481 tctcttgtat gaactaaaag atgagttttc atataccatc gcacccgcat tgacctttac
      541 cgcgcaagtg gcagccattc atgatggatc ttttcaatta gaagacctgg tacaaggttt
      601 ccgtgacatt gaacaatcca gcgaaaactt tgagaactta tttgaagaca ttgatctgta
      661 ttctaaaaaa ctggggtcaa ccccacaaaa acaaaataaa accatcgcag atgttatgaa
      721 agaattagaa gggttaaaca tggcggggca tgctggcgat gtcttaggcg atgcctatga
      781 gtacttaatt ggccaattcg catctgaatc cggtaaaaaa gccggtgaat tctatacccc
      841 acaaccggtc gcaaaattga tgacacaaat tgttttacaa ggaaaagaag ataaaaaagg
      901 cttttctgtt tacgacgcga ccatgggttc gggctcgtta cttttgaacg ctaaaaaata
      961 ttcccatcaa ccgggaacca tttcttattt cggacaagag ttgaatacgt ctacctataa
     1021 tttggcgcgt atgaacatga ttttgcatgg ggttcccatt gccaaccaac acttgcataa
     1081 tgcggatacc ttggatcaag actggccaac agaagagcca acgaacttcg atggggtgct
     1141 catgaaccca ccgtattctg caaaatggtc ggctgataaa ggcttcttag atgacccacg
     1201 cttctctgcc tatggcgtat tagcccctaa atcaaaagcg gactttgctt tcttattaca
     1261 tggctactat catttgaaag atacgggcgt catggccatt gttttgccac atggggtgct
     1321 ctttagaggg ggcgcagaag ggaagatacg caaagccttg ttagaaaatg gtgccattga
     1381 caccgtcatt ggcttacctg ctaacatctt ctttaatacg tccattccaa cgaccgtaat
     1441 tatcttgaag aaagaccgtg cgaaaaaaga tgtgctcttt attgatgcgt cacaagattt
     1501 tgaaaaaata aaaacgcaaa atacgctacg agattaccat attgacgcta ttttagaggc
     1561 ctataaaacc cgtacaatta aagacaagta tgcttatgtc gcagaatttg atgaaattgt
     1621 ggagaatgat tacaacttaa acattccccg ttacgtggat acctttgaag aagaagaa
//
LOCUS       DAXWLG010000284         1624 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXWLG010000284 DAXWLG010000000
VERSION     DAXWLG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.292"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..482
                     /locus_tag="ACGE4T_002774"
     CDS             <1..482
                     /locus_tag="ACGE4T_002774"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387973.1"
                     /translation="IKRKLFGKNLRECINEGLIFGKKHSLVEVPEFGSEPYLIEIGDN
                     FRCSFGVKFITHDGGVWVLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDN
                     VIIRANTLVNKDIPNNSVVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            570..>1624
                     /locus_tag="ACGE4T_002775"
     CDS             570..>1624
                     /locus_tag="ACGE4T_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFF8387974.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLK"
BASE COUNT          587 a          212 c          325 g          500 t
ORIGIN      
        1 gaataaagag aaaactattt ggaaaaaatc tgcgagaatg tattaatgaa ggtttgatat
       61 tcggaaaaaa acatagttta gtagaggttc ctgaatttgg gagcgagcca tatttaattg
      121 aaatagggga taatttcaga tgttcatttg gtgttaaatt tataacacac gatggtggag
      181 tctgggtctt aaggaagatg agttctgtga gcgagtcaac ttcaaaaata ggtaggatca
      241 aattgggaaa taatattttt attggtattt actctattgt tttcccagga gtttcaattg
      301 gcgataatgt aattattaga gcgaacactt tagtaaataa agatatccca aataatagtg
      361 ttgtagcggg tagttcatgt aaagtgattt caactataga ggagcattat gagaaaaata
      421 aaaatgaatt tattaaatat cgaacctcga tgattttgaa gcaaaaaaat tttttctatt
      481 aaataatatt gattgaataa ggaatatgtt caaaaggata acagctaaaa tactataaaa
      541 tgaaatttga ttggagcgta tggaagaata tgaaaatttc agttttcgga ttaggctatg
      601 ttggtctagc aaattcacta ttattagcac aaaaagagca ggtcaaagct tatgatatcg
      661 tggaagaaaa aatcacgatg ttacaaaatg gcatctcacc aatagaagat aaagaagttc
      721 atgaattttt aaaacgggat gacttaaatg tcgaatttac gtcaaatttt actgatgcag
      781 taaacttcgg tgattacttg attattgcaa ctccaacaga ttatgacgaa aagaaaaact
      841 attttaatac atcaacagta gaagatgtaa ttgagaaggc tttagcaatt cgtccagatt
      901 caacattcat tattaagtcg acagttccag ttggctatgc aaatgaaatt aaagcgaaat
      961 ttggtacaga aaacatcatt ttttcacctg aatttttgcg tgaaggaaaa gctttgtacg
     1021 acaacttata tccatcaaga attatagttg gagaatgttc tgctcgtggt cgcgagattg
     1081 caactatgtt tcaaagaaac gcatttaaca aagatgcatc agttcttttg actcactcaa
     1141 ctgaagcaga agctattaaa ttattctcga atacatattt ggcattgcga gtggcgtatt
     1201 tcaatgaatt agatacgtat gcagaatttc gtgggcttga tagtaagcaa attattgaag
     1261 gtgtcggttt ggatccacgg atcggttcgc attataataa tccttctttt ggctatggtg
     1321 gatactgttt acccaaagac acaaaacaat taagagccaa ctatgaaaaa gtaccaagca
     1381 atatcatcag tgcaattgtt gatgcaaata caacaaggaa agattttatt gctggacaga
     1441 ttttggcgaa aaaaccaaat gttgttggaa tctatcgttt aacgatgaag gccggttcag
     1501 ataattttcg atattcgtcg atccaaggaa ttatgaaacg gattaaagcc aaaggaattg
     1561 aagtggttgt ttacgagcct acattggatg aagatgaatt ttatcattca caagtattga
     1621 aaga
//
LOCUS       DAXWLG010000285         1619 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXWLG010000285 DAXWLG010000000
VERSION     DAXWLG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.293"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..839)
                     /locus_tag="ACGE4T_002776"
     CDS             complement(<1..839)
                     /locus_tag="ACGE4T_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFF8387975.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSF"
     gene            complement(998..1264)
                     /locus_tag="ACGE4T_002777"
     CDS             complement(998..1264)
                     /locus_tag="ACGE4T_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8387976.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1254..1610)
                     /locus_tag="ACGE4T_002778"
     CDS             complement(1254..1610)
                     /locus_tag="ACGE4T_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8387977.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          457 a          317 c          197 g          648 t
ORIGIN      
        1 tcaaaagaag aatcgtattc ttcctttaaa attcccgatt tacttaattt ttctagtaca
       61 tcaaatactc tatcactcaa gtaactcctt aaattgttat cactttccca ttgaactact
      121 tttctataag ttgaattatc agttgaaaag atagaatttt catccataaa cgcatatatt
      181 agcagcaatc tttcatcatt gtcaaattct attggatcaa aaatatctaa gttgtcatca
      241 aaatctttat cagttccggt aacttttttg ttaattactg aaggtgtctt gtaagaaata
      301 gaatctaaat caaagcgttt tgattctaaa ttattttgtg ctttctcaat taaataattc
      361 actttttttg tgagaaacct tctgatattg tctttgctat atgttccata aaaaccctta
      421 tcttctaaag agtccctgta cttcttaact tctatatact cttcagccaa aacttgtgga
      481 actggttcat ctaagaaaaa aacccctacc tcgcctttgc tatttttata aaagtaatca
      541 atttctgcta tagttcccga tctataatta tcaactggag aacccaactt atggccaaaa
      601 atcgctaata atatatcact attttctaca atttgtttat taataacact ttgcccagag
      661 ctattattta tataagtagg aatcgcattc gtttcccatc gaattggcat caaaattact
      721 tttgtattat ctgtattttc tatattccat ctatatatgg tttcttctac ttcattcctc
      781 tttgctgcta tatcacctgg tgatgctata aatactttta ttagatttat ggttttcatt
      841 tattgtacct ttctctttat gtctctttaa agtaattttt gtataaaatt tattgacttt
      901 tacactatag gacaactttt atctatttag tacaaaaagg cccactaaac taattgatta
      961 gttaggagcc tattacaggt atattttcaa ctcgctttta agcaaattct actaccagct
     1021 ttttgcctaa tgccctagcg atatcatcaa gtttatcgaa agatacgtta tcccctctct
     1081 caattcgagc aatagtagat tgagttgtat gtgctttttg tgcaagctcc gcttgagtta
     1141 ggcctgcttc ctcccgtgca tgataaagag caaccgcagt ttcgagtttt cttccttcac
     1201 ggtcatatgc ttctgcaaaa tctttgtctt tttgcttttc ttcaatcaat tttttaatca
     1261 tcatttgtga accactcctc tctaagttct tttgcatgtt taagttcact tattggtgtt
     1321 ttctccgttt ttttagtaaa tccatgggta atgacatacc ttccattgac cacatggaaa
     1381 tagatagctc tttggatgtt tgatcctacc ttacttctta attcgtacaa attagtgtct
     1441 aattttttca cccatttcaa ccgttgagct actagcatcc cattcttttc tgtttcttct
     1501 attgtacgta aaagcttcgc actatctttt tttggtaatg tttccaacca ttctaaaaat
     1561 tcatcatgac cattaggtct tttaaatgtt tcaaaagata ttttttccat acccaatta
//
LOCUS       DAXWLG010000286         1603 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXWLG010000286 DAXWLG010000000
VERSION     DAXWLG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.294"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            92..1558
                     /locus_tag="ACGE4T_002779"
     CDS             92..1558
                     /locus_tag="ACGE4T_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFF8387978.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          446 a          292 c          334 g          531 t
ORIGIN      
        1 agtttatatt tcttaaataa gttttttagt taaatttttc ggtaaagatg tataatattt
       61 tctgaaaatt taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt
      121 cgggcagccg aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta
      181 ttacggtatg cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact
      241 tggattacca cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat
      301 gtctagtatc gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt
      361 cttcggtgga gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag
      421 tacattgttt gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt
      481 ttctggcatg gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc
      541 gattttttac atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat
      601 cggtcaagaa tataactatc atttagggtt ctctttagct gctattggga tgttctttgg
      661 attattacaa tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc
      721 gaatcctttg acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt
      781 tgttatcgct gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt
      841 tgtcaataca atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct
      901 gacttcaaaa gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt
      961 ccttgcagca atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt
     1021 tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca
     1081 atcattgaat ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa
     1141 acttggtgaa cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg
     1201 attatcgtac ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag
     1261 tccattgtgg ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc
     1321 agttggtttg tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc
     1381 tatttggttc ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta
     1441 cacaccggga acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg
     1501 tatcttgcta tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc
     1561 aatgaacata aaaacaggaa ttgcttattt gcagcagttc ctg
//
LOCUS       DAXWLG010000287         1599 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXWLG010000287 DAXWLG010000000
VERSION     DAXWLG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.295"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            171..509
                     /locus_tag="ACGE4T_002780"
     CDS             171..509
                     /locus_tag="ACGE4T_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387979.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(714..1276)
                     /locus_tag="ACGE4T_002781"
                     /pseudo
     CDS             complement(714..1276)
                     /locus_tag="ACGE4T_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT          497 a          323 c          268 g          511 t
ORIGIN      
        1 aaataaattg tagccagttc attttttctt taactaaaaa aataatccaa gaaacattga
       61 acctatggat ccaaaagcta cccaaatact atagctaacc ccccgctacc ttcaattttg
      121 cagataaaac gctttgggag aaaatttcaa agacaacagg ccatcctatc atgcccgatt
      181 ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga aagttcacga
      241 aactagcagg tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt caaatagaaa
      301 cattggttca acgaatttca actagacaag actttgctat tgtagaacgt gagatcgtag
      361 ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg gataaatggc
      421 aacctcttat ctataaattc agagaatata caactgccaa ccagtcgtta actttgaatt
      481 ttagattcca agaattcaaa gagtcataat gataaacatc gttaggtata aataaaaaag
      541 actcgcagca cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg actgtgaccc
      601 tatagatgca gtatatgcag cttatgagta tttcttctgt tctctttgtt tttgggatct
      661 gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt atgttatacc
      721 ttctccatta ctaattcagg catctgctga gtaatgattt ttcgcgcaaa agaaatatct
      781 gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc atggattggt
      841 gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt ttcatcctga
      901 tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa cataaatttg
      961 tctttttctt cattccactc attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa
     1021 aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt ccttttttaa
     1081 atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc tcttttctta
     1141 acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct gcctgcttcg
     1201 gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct agatcaaaaa
     1261 aataacgacc acacatccta atccctccca atcttttttt ctatcataca ctgcaacaag
     1321 aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg tgacaagtga
     1381 caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc acaatattca
     1441 acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga aacggcaggc
     1501 ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat
     1561 gcaaaaatgt agaaagctat tttcacaaaa tttttctta
//
LOCUS       DAXWLG010000288         1590 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXWLG010000288 DAXWLG010000000
VERSION     DAXWLG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.296"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(451..>1590)
                     /locus_tag="ACGE4T_002782"
     CDS             complement(451..>1590)
                     /locus_tag="ACGE4T_002782"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8387980.1"
                     /translation="SHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSA
                     KDILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYH
                     AEHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYEC
                     DLACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIH
                     DIDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDL
                     AQMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIED
                     VCFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT          447 a          334 c          248 g          561 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact
//
LOCUS       DAXWLG010000289         1536 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXWLG010000289 DAXWLG010000000
VERSION     DAXWLG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.297"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            191..445
                     /locus_tag="ACGE4T_002783"
     CDS             191..445
                     /locus_tag="ACGE4T_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8387981.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACGE4T_002784"
     CDS             446..763
                     /locus_tag="ACGE4T_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8387982.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..>1536
                     /locus_tag="ACGE4T_002785"
     CDS             1322..>1536
                     /locus_tag="ACGE4T_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387983.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISS"
BASE COUNT          542 a          221 c          293 g          480 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctca
//
LOCUS       DAXWLG010000290         1524 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXWLG010000290 DAXWLG010000000
VERSION     DAXWLG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.298"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(61..1191)
                     /locus_tag="ACGE4T_002786"
     CDS             complement(61..1191)
                     /locus_tag="ACGE4T_002786"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFF8387984.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1251..1478)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          425 a          311 c          243 g          545 t
ORIGIN      
        1 ttctccactc catgaaaagc cgactttttg ggacgctttt ttttgccaag cctcacattt
       61 ttatagccaa acttcttttg cgacttgctg caataattgg attttcttcc attgttcttc
      121 ttctgttaaa tggtttcctt cttctgttga agcaaaccca cattgaggac tgatacttag
      181 attttccaat ggtacaaatt gttgtgcttc ttttattcgt tcttttattt tctccgtatc
      241 ttccaaatct ggaaacttag aagtaactaa tcctaaaaca actttacttt ctttgttact
      301 ccaatgtctt aaaggagtaa aatctcctgc tcggtcagaa tcgtattcca aaaagaaacc
      361 atcataattt gttgaaaaca actcttctgc aatgggttca tatccaccgg aaaacagcca
      421 agaagaagcg tgattcccgc ggcagatatg tgttgtgatc gtcaaatctt ctggtttgtc
      481 ttccagtgcg ttattgataa tctctgtaca aatatgggcc atttctttcg gatcaattcc
      541 tttttgaaga agtgcttctc ttttagtttg atcagctaga aacgcccaat ttgtatcatc
      601 gaattgcagg taacggcaac ctttttcata aaaagcatga atcgctgctt tgtaagtaga
      661 ggacaagtcc tcataaaaat cagatagatc aggataaatc tccttgattt ttgaagaacc
      721 atcattagat aggatctctt gtcgtaaaat catcgtggga ctagggatcg ttgcttttgc
      781 aacaaaagta tcatctgttt ccactacttc tttcagaaaa gcaaaatctt ttaaaaatgg
      841 gtgatttgga ttgaaagata atttatctac tacccgaata ttataagctg gtgctgcttt
      901 tccgtggaat gtttgattat atccagtttc tggaacatag ccttcgatac catttagttc
      961 ttctaaaaaa tcaagatgcc aaaatccacg ccgaaattca ccatctgtaa ttcctttgat
     1021 gccgactgct ttttggtctt ccactaattt ttttatttcc tggtcttcga ttgcacgaag
     1081 ttcttctttt gaaagtaagc cttcttgaac tttttttctt gcttctttga tcgattgcgg
     1141 gcgtaagaaa cttccgacat gatctactgt aaatggtcta tttttgctca tttcactcac
     1201 tcttttcttt gttttttgta aaaaaaaatc cttttactag caaatactag taaaaggacg
     1261 attatactcg tggtaccacc ttttttcaga aaaaacattc tttctctcag cctgacaata
     1321 aaatcagtgg tcttggtaac gaaagactgt tcgtttttgc ctacctttcg acaaaaaatc
     1381 ctctaaggcc attttcgaca gtcaacttct agctttttac accaaccaaa gcctctcttg
     1441 aaccattttc tgtctacttt ccttctcatc ggatactatt agaataaaat tagaatagca
     1501 tgtttgaaag aaaagtcaat aggg
//
LOCUS       DAXWLG010000291         1521 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXWLG010000291 DAXWLG010000000
VERSION     DAXWLG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.299"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(17..1048)
                     /locus_tag="ACGE4T_002787"
     CDS             complement(17..1048)
                     /locus_tag="ACGE4T_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8387985.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          458 a          241 c          260 g          562 t
ORIGIN      
        1 agatcataca tttgttttat atggaagaaa cccgaacgaa tgccgctaat tcgtgataag
       61 aaaaatcaac catcttttcc cagatatctt gaaaagaatg ggatgagagg tatcgattac
      121 cacataaata atttaccgcg tcagcatcat ctaaatatag ttctgcataa taacgatatg
      181 tttttctgca tagttctggc gatacgtttt tatatactgt aattttgctc aaaatttggc
      241 gaatagtccg aggagaaatt tgctgatcat gtgaggaacg aaaaacaaaa gaattttcgt
      301 ccgtattttg tgctgttaac ttaaaatagt tctccagaaa tggcatcgct tcttttggta
      361 ttggtacttt attcagtctg ttttcttttc tattcagatt aagaatctgc ttggtcaaag
      421 atacatcttt acgtttaagt tctactactt catggatctt taatccgcaa tacagaagaa
      481 tggcagtaat tgcaaggttc ctcggtctat tcttttcttc aaagctagct tctctgcttg
      541 tttgaaagcc ttcaatcggg ataaacaaat gctcaaaatc tttgaaaagt tctgcatcga
      601 tatatcgcgg agctgctttg gttgtttccg gtaaacttgt acccaaaaga gaaatctctt
      661 cttcaatcag tcctttttga tacaaaaatc gtaaaaaatg atttaatgaa tattgcttcc
      721 ttgaaatccc gtaatagcta ttttcaaaca attgctccgt ttcttggttc gctgtagttt
      781 ggaacgaacg agtatgtcga tagattttag ctaagaaatt ctgataatct tgtttttcta
      841 aatggataat cagctgatca tccacttctt cagaaaatac ttttctcaaa taatcagtaa
      901 acagatgaaa atcttttgcg tattcatagc gactgcctaa tgaaagggaa gaacgatttt
      961 cttctaaaaa ctgttgaaag acggaaggga gcaagtgtgt acgttgcttt aacttttctt
     1021 gcagttgttt tctagttttg ctgtccatta caaattccct ccaatagtgt tcattttcta
     1081 tattttaccg catttcatca aatgaagata gacagtttgt cttgatctta ggaagcaaat
     1141 ttttaactaa gtactagcta aaagatatga agtaagaaga tacattttta gttattttat
     1201 tagtgcagtc attcttttga tcttttaatt gatttatcct ctttttagct atgtggttac
     1261 ttcatgagca atgaaatgga tttggttcgt tctaatggca ggaggctctt tcttttattt
     1321 ccgaaaaatt agaaaacatt ttttcagaat cgattggctc tccatttatt caataatggt
     1381 ttgtctaacg atattgttgg gataattctt aaagaagtat tagaaatagt tggtacttca
     1441 ttactttaca tgaagattta ttctatggta ggagcaatta tattgagaat taatcctttt
     1501 ttatataagt tgtttgctta t
//
LOCUS       DAXWLG010000292         1519 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXWLG010000292 DAXWLG010000000
VERSION     DAXWLG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.300"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            47..469
                     /locus_tag="ACGE4T_002788"
     CDS             47..469
                     /locus_tag="ACGE4T_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387986.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            479..682
                     /locus_tag="ACGE4T_002789"
     CDS             479..682
                     /locus_tag="ACGE4T_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387987.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            893..1477
                     /locus_tag="ACGE4T_002790"
     CDS             893..1477
                     /locus_tag="ACGE4T_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8387988.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          537 a          220 c          282 g          480 t
ORIGIN      
        1 ttattaagaa aacgttttct tatttttgat ttcgaggtga tagacaatga aaaataagtt
       61 tattttatct ttaggtattt taactattgg aattagtgta actggaacta caattcctac
      121 atttgctgat agtacaaatg aaccaactac tgtagtagat aataaccaag aattaactcc
      181 tttagatagg aacggaatag ccattggtaa cccaactaac gacttaactg ttactgaaaa
      241 ttatttaata gtttctccat tacttgctcg agcagcttct aaaacgatac gagtgacagg
      301 aacgatgatt tttgataaag ttggttggcc ttctagacct cctcaatcac ggaattggaa
      361 agaaaaaaga aatggttatt ggtactatgg aaaattgtat ttacaaagtt ataaatcagc
      421 tggaaacaat tggattgcga cttattcagg aaccttaact agaaagtagg gattttttat
      481 gccagaattt gtgaacacac caaattttta tcaagatagc gatattctaa gtaaaattaa
      541 tttaccgaaa gaagaatcag ctttacaagt tcagcgaact ttacaggaga cacaaaaaat
      601 tcaaaaatca atttctaatc tagaagattc tgaagaaaaa agaaaaaaaa gagaccaaaa
      661 aacatatcaa aaagaaaaat aatgatttta aaaaaagagt gtctagtcga cattcttttt
      721 ttctaattga aagtgttcat aaaatcaccc atttctggac actttctttt tttgctttat
      781 ttttcaaaaa aacaattcat ttcatgttca taaacccata acaatatcta tgttcagtta
      841 aactatatgg ataaagttat gttacaataa aattaatgaa ggagtgattt ttttgaaaat
      901 aggatacgcc cgggtatcta ctggtttaca aaatttggat ttacaagaag acagcctaaa
      961 taaatttggt tgtgaaaaaa tttttactga tcatatgagt ggggcaaaaa gtaatcgacc
     1021 aggattagaa atggccattg attttgttcg ctctggcgat acacttgtcg tttggcgctt
     1081 agatcgttta ggtagaaata tggaagattt gatttcaatc gtcaatcgac tgaatgagcg
     1141 tggtgtgagt tttcatagta ttcaagaaaa tattacgatg gacaaatcaa gttctacagg
     1201 acagctcatg tttcatttgt ttgcggcatt tgcagaattt gagcgtaatt tgatcctgga
     1261 acgatccgca gcaggacgag cagctgctcg tgcaagagga cggtttggtg gtcgtcctga
     1321 aaaattgtct gaacaagatt tggaattact gaagacttta gtggatagcg gtacacctat
     1381 taagactatt gcggaacggt ggaacgtctc aagaaccacc atttatcgtt atttggcaaa
     1441 aatggaggag aaaaatggaa agacaaattc tcattagttt ttgtcattta atgctgaaaa
     1501 tttttgccat ttatctaga
//
LOCUS       DAXWLG010000293         1504 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXWLG010000293 DAXWLG010000000
VERSION     DAXWLG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.301"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(106..867)
                     /locus_tag="ACGE4T_002791"
     CDS             complement(106..867)
                     /locus_tag="ACGE4T_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFF8387989.1"
                     /translation="MNQPQIFNFEHNEVRTFLENDVPYFVANDVAKTLGYKNPSKATN
                     DHCKKAIETWGNDSLGRRQKFKIIPESDVYRLIIKSNLPSAEKFESWVMEEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQQVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            complement(916..1149)
                     /locus_tag="ACGE4T_002792"
     CDS             complement(916..1149)
                     /locus_tag="ACGE4T_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8387990.1"
                     /translation="MSTMEKKKNLRVEFLKPKLKLKKARLANEWTTSYVADLIGLERR
                     QYELKEKGEYPFHDYEMYILAKNFNKKVSELFF"
     gene            1291..>1504
                     /locus_tag="ACGE4T_002793"
     CDS             1291..>1504
                     /locus_tag="ACGE4T_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8387991.1"
                     /translation="MKKNILGSVIKDARKKKKLTQEQLSKLTGYSQNTISNHENGNRS
                     LDEDNIKTYATALDLTPDDLFEALDIK"
BASE COUNT          415 a          273 c          240 g          576 t
ORIGIN      
        1 tttccaaaag aatagtctat gaagccagct catttttgag ttggtttttg taatttttag
       61 gtcaatggtt acgcgccctt ttggtctctc cattcatctc acctcctatg ctgtttgttc
      121 acgttcaatc atcggtaagt aattattctt cttcaatgtt tcatataaga aaatgtgtcc
      181 tttttgcgtc catttcatcc ttgtttttac ttgccgattg ccgtttttat cttcataatc
      241 aaatggctca atatgcgtgt atcctttgtc ttggaacttg ctgtatagca gccatgtgcc
      301 actttgattg aactggatac ctaattcatg tagcaattta ttaaacgata cagcgctcat
      361 accgtagttc ttagcaatcg tactaactgt aactaatcct ttgttgttca agattcggtc
      421 gtaatagtct gccttcggct gtaattcatt tacttgttgt tctgctatca atcgtaacgt
      481 gcgttcttct ttgagtttgg ttgctacttc gattagcaag tctggattgt taagtagttc
      541 atctgttgcg tacatgccgt gttttcggat ttgaggtagg acttcttcca ttacccaaga
      601 ttcgaatttc tcagcgcttg gtaaattcga tttgataatc aagcgataaa catctgattc
      661 tggaatgatt ttgaactttt ggcgacgacc taacgaatcg ttaccccatg tttcaatcgc
      721 ttttttgcaa tgatcattag tagctttgct tggattttta tatcccaaag tttttgcaac
      781 gtcattcgct acaaaatatg ggacgtcatt ttccagaaac gttcgaactt cgttatgttc
      841 gaaattaaaa atttgtggtt gattcatttt tttacattcc tttcttgttt ttatctcaaa
      901 tagagatatt tgattttaaa aaaataattc tgatactttt ttgttaaagt tttttgccaa
      961 aatgtacatt tcataatcat ggaatggata ctcgcctttt tcttttaact catattgacg
     1021 tctttccaaa ccaatcaaat cagctacgta agatgtagtc cattcatttg caagcctagc
     1081 ttttttgagt tttaacttcg gcttcaaaaa ctcaactctt aagtttttct ttttttccat
     1141 cgtactcata taatcgcctc cttcatttga taaatcaata atatctcata ttgagatata
     1201 tgtcaatagt tttttatcac tttttgagat atttttattt acacgtttat cacaatgtga
     1261 tattgttatc tcatgaagga ggtgccttta atgaagaaaa atattttagg ttcggttata
     1321 aaggacgcaa gaaaaaagaa aaaacttact caagaacaac ttagtaaatt aactggctac
     1381 agtcaaaata ctatatctaa ccatgaaaac ggaaaccgat ccctagatga agataatatc
     1441 aaaacttatg caacggcttt ggatttaact ccggacgatc tttttgaggc gttagatatt
     1501 aaaa
//
LOCUS       DAXWLG010000294         1503 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXWLG010000294 DAXWLG010000000
VERSION     DAXWLG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.302"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            131..505
                     /locus_tag="ACGE4T_002794"
     CDS             131..505
                     /locus_tag="ACGE4T_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8387992.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACGE4T_002795"
     CDS             complement(619..1248)
                     /locus_tag="ACGE4T_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8387993.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACGE4T_002796"
     tRNA            complement(1382..1454)
                     /locus_tag="ACGE4T_002796"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          509 a          227 c          263 g          504 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tca
//
LOCUS       DAXWLG010000295         1500 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXWLG010000295 DAXWLG010000000
VERSION     DAXWLG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.303"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(121..555)
                     /locus_tag="ACGE4T_002797"
     CDS             complement(121..555)
                     /locus_tag="ACGE4T_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8387994.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(655..>1500)
                     /locus_tag="ACGE4T_002798"
     CDS             complement(655..>1500)
                     /locus_tag="ACGE4T_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8387995.1"
                     /translation="KETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGIL
                     LEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITSD
                     RLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSL
                     NLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGR
                     GLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFT
                     YHPIE"
BASE COUNT          531 a          296 c          238 g          435 t
ORIGIN      
        1 agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca gacgcgcttt
       61 ctcgaatagc aaacatatac aaaaaaatat cagagacaaa aactaatctc atcataaaca
      121 ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa cttacttcac gatggacctg
      181 atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc
      241 catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt
      301 gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg
      361 agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtgc catacaaggt
      421 aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc
      481 aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa aatctatttc
      541 cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt
      601 attataccag ttaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc
      661 gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa
      721 ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac
      781 aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt
      841 aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact
      901 catgaccata acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga
      961 agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa caaacagatt
     1021 aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata
     1081 ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa
     1141 aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga
     1201 agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaacaagc ttccgaaaag
     1261 tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt
     1321 caacactaaa ataactatag ctcggtcaaa tcctaaaaca gcttccagta atattcctag
     1381 accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc
     1441 taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt
//
LOCUS       DAXWLG010000296         1497 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXWLG010000296 DAXWLG010000000
VERSION     DAXWLG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.304"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(58..942)
                     /locus_tag="ACGE4T_002799"
     CDS             complement(58..942)
                     /locus_tag="ACGE4T_002799"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8387996.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1037..1474)
                     /locus_tag="ACGE4T_002800"
     CDS             complement(1037..1474)
                     /locus_tag="ACGE4T_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8387997.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT          467 a          322 c          230 g          478 t
ORIGIN      
        1 tcccttatcc cgaacaagcg gtgttctccc gccaactcct cggtcacaac ctccatttta
       61 acaataaacc tgtaaatgtg agggcaatac tttgattgtc acaggtaatt tatccccttc
      121 atctccatca acattcgtcg tcaattcttc ctcatcagct aatgacacaa ctaattgctc
      181 acaagtaagg taggctagat ttttcgtaga ttcatctact ccttttaata ggtctggcaa
      241 tgttttaagc gtatcgatca aagaagaatc ttttaaataa accaaatgga gcttaccatc
      301 atccactttt gcatttggaa gtaatgtttc aaaaccgccg actgagttag ttagtcctat
      361 cagcaaggta ctgctctcaa tttcttcctt ctttccatca acttctacgt gaaacgagta
      421 ggattgtgtg gaagctaact gtttgattcc tgacatgaaa taagctagtt ttccaaattt
      481 cgtcttcttc tccgcatcta catcattgat tgattcaggg atagagccaa ttgcgacgac
      541 attcataaaa tagtcgtcat ttatttttcc aatatccaat ggcttgactg attcaatcga
      601 aaaatgattg atcgcttcct caggttctag cggtatccct aatgctcgag ccaagtcatt
      661 caccgtaccc aatgggaaaa atccgaaatt tggccgatgt tcttgttcag cgattccact
      721 gattccttcg ttgactgttc catctccgcc catgacaaac acactatgat acccttccgt
      781 agccgcttcg cgagtaaagt tttttgcatc ccctgctttt tttgtatgca agacgaccac
      841 ttcatcaaac accgattcca attttgctat cgctaattgc tcaaattcct ttgcttgttc
      901 accgcctgaa ctggggttta ccactaataa aacttttttc atcactttct ttcccttctg
      961 attgatttct ttaatataac gatagcataa agaaaaaaat taggctgtga tgaagtactg
     1021 ttcatgaaac attcatttat tttttgacaa gataaaacat ccatactatg aaactgagga
     1081 aaattaaaac aatgatatag gaaataaaag aaaatgatag gcattgtata cctaataaag
     1141 taaaaatctt aggtggcaga taaaaacaca aaaatagcaa gagaatgtaa tagcgtttct
     1201 tccacttttt gttatcagac cttatagcga tcagaggaat accaaatgca taactgaaaa
     1261 actcttgatg tactgttaat atgtcacggc cttgaataat aaatgcgaca atccccgcta
     1321 taattagtgt gcctaacacc aaaacggaaa attgatgaat atattttata tacaaatgat
     1381 ccattgtact tcctgccttt ttatttgtga gaaaaacaaa tccgtatacg tgaataactt
     1441 ctagaaataa aagaaatttg caaaaccact ccatttatct acttctacgt gcatttt
//
LOCUS       DAXWLG010000297         1493 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXWLG010000297 DAXWLG010000000
VERSION     DAXWLG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.305"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            197..811
                     /locus_tag="ACGE4T_002801"
     CDS             197..811
                     /locus_tag="ACGE4T_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8387998.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(835..1185)
                     /locus_tag="ACGE4T_002802"
     CDS             complement(835..1185)
                     /locus_tag="ACGE4T_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8387999.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1179..1442)
                     /locus_tag="ACGE4T_002803"
     CDS             complement(1179..1442)
                     /locus_tag="ACGE4T_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFF8388000.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          481 a          277 c          236 g          499 t
ORIGIN      
        1 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacaca
       61 tactatttcc tccatcatta gtttttgtct atataaggta taccctaact agacacgacc
      121 gcgacattga aaaatctggt tttctcgtgt atgattaggg tgttttttaa atagttgcag
      181 aaagtaggta aaaattttga gagttgcata catacgtgtc tcatccatcg atcaaaatga
      241 acaacggcag attgaagaaa tgaaaaaatt cggtgcagag agaattttca ttgagaaaca
      301 atctggcgca acgattactc atcgacctgt ttttcaagaa gcactggatt tcgtccgaga
      361 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattacg acgagatcat
      421 cgcttcagtc aattacttga agaagaaaaa tgtgcagctg atcatcacaa gtctaccgat
      481 catggccgaa gcaattggca atcctctcct ggataaattc attaaagatt tgatcattca
      541 aattttggcc atgatcgctg aacaagaacg taccgaatct aaacgacgtc aggcacaagg
      601 cattaagatt gcaaaagcga acggtgttta taaaggacgt cctaagctat acagtgccaa
      661 tgcaaaagat ccccaacgtc gtcttgttta taaaaacatc gttgaggatc taaaaaaagg
      721 tgttgcgatc gctaagattg caaaagacta caatgttacg agacaaaccg tatatagaat
      781 aaaaaaagac agcatggtca atcacgaata gttgatcatg ctgctgtttc tattttattt
      841 gcttactttt tgaaaaactg ttctaacttt catcaataac tcctcaacta aactatcatg
      901 cgctttttcc aaaaaaatac actctctggc ttcataatca attgtcgtta gttgatctaa
      961 taatactttt cctgttgtct tcatttcata ttctgagttt atatttacat atagcggata
     1021 gtttctagtt gtactagaaa ttggagcaac tgtaacaacg ttactataat ccgcaataat
     1081 atcatgactt aaaacaatat aaggtctttt acctgtctgt tcgtgtccag atcgaggtgc
     1141 tgtattcaat aatagaatat cgccttgtct aggttttctc accatttttc attacctact
     1201 gcttcaccta aattttgaat cgtcacatga tttctttcat cagtgtaatt ttcaaataac
     1261 tgttcaattg ttaattcatt ttctatatta gccgcttcga taattagccg cttctgaccg
     1321 tttacttctt cttgtattat cttcactttg tcttctgtat gaattccaag atattccata
     1381 atatactttg gcaatctaag gccattacta tttccccatt tttttatagg taacatttcc
     1441 atagtagaca cctctttctg tatatacata gtataacata cataagaaaa aac
//
LOCUS       DAXWLG010000298         1457 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXWLG010000298 DAXWLG010000000
VERSION     DAXWLG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.306"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(65..137)
                     /locus_tag="ACGE4T_002804"
     tRNA            complement(65..137)
                     /locus_tag="ACGE4T_002804"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Gln,seq:ctg)
     gene            complement(183..611)
                     /locus_tag="ACGE4T_002805"
     CDS             complement(183..611)
                     /locus_tag="ACGE4T_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388001.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(671..1429)
                     /locus_tag="ACGE4T_002806"
     CDS             complement(671..1429)
                     /locus_tag="ACGE4T_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8388002.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          414 a          281 c          275 g          487 t
ORIGIN      
        1 ataatccccc tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat
       61 caaattggga tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc
      121 agtttggcta catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca
      181 agttattttt cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc
      241 ttttctaatc cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac
      301 tgatattctt tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat
      361 tcaaagcgag tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg
      421 tactcttgat ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg
      481 agggtagcag gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt
      541 cccttgcagt cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg
      601 aatttttcca ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt
      661 gtttgaatta ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg
      721 ccgaaaaatc gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa
      781 tattttcttt gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt
      841 ctccattatt tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag
      901 tcacagaagt ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag
      961 catcggtttt tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa
     1021 ctgggggaac atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt
     1081 cattaggatc gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag
     1141 ctaactggtt tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga
     1201 cttcacagcc attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta
     1261 aaacttctgc tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt
     1321 tcattgtatg ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag
     1381 cagctgtgcc ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc
     1441 cttttcagct gtttatc
//
LOCUS       DAXWLG010000299         1457 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXWLG010000299 DAXWLG010000000
VERSION     DAXWLG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.307"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(100..1119)
                     /locus_tag="ACGE4T_002807"
     CDS             complement(100..1119)
                     /locus_tag="ACGE4T_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8388003.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1130..1336)
                     /locus_tag="ACGE4T_002808"
     CDS             complement(1130..1336)
                     /locus_tag="ACGE4T_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8388004.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          403 a          304 c          246 g          504 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg
     1141 atgaaatttt cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa
     1201 aacctgtaat aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa
     1261 gaccgacaaa agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa
     1321 aggttctatt gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat
     1381 cgcttgtttt cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc
     1441 agttgttcaa ttttatc
//
LOCUS       DAXWLG010000300         1452 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXWLG010000300 DAXWLG010000000
VERSION     DAXWLG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.308"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..613)
                     /locus_tag="ACGE4T_002809"
     CDS             complement(<1..613)
                     /locus_tag="ACGE4T_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFF8388005.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..>1452
                     /locus_tag="ACGE4T_002810"
     CDS             901..>1452
                     /locus_tag="ACGE4T_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8388006.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPY"
BASE COUNT          502 a          213 c          276 g          461 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata
     1321 aatggtgaag aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat
     1381 aataatggac aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa
     1441 gaaactcctt at
//
LOCUS       DAXWLG010000301         1449 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXWLG010000301 DAXWLG010000000
VERSION     DAXWLG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.309"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            98..1390
                     /locus_tag="ACGE4T_002811"
     CDS             98..1390
                     /locus_tag="ACGE4T_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFF8388007.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          470 a          298 c          258 g          423 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctccaattat tctaagattt
     1441 ctctttacc
//
LOCUS       DAXWLG010000302         1394 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXWLG010000302 DAXWLG010000000
VERSION     DAXWLG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.310"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            33..1372
                     /locus_tag="ACGE4T_002812"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACGE4T_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFF8388008.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          579 a          181 c          244 g          390 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttc
//
LOCUS       DAXWLG010000303         1388 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXWLG010000303 DAXWLG010000000
VERSION     DAXWLG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.311"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            114..>1388
                     /locus_tag="ACGE4T_002813"
     CDS             114..>1388
                     /locus_tag="ACGE4T_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8388009.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQ"
BASE COUNT          499 a          195 c          256 g          438 t
ORIGIN      
        1 aataactgat gaaaaaacaa gcataatcca tcataaaatc agtataggtg aaaagatgaa
       61 ctttcatctg catatcaagt aattggtatg aggcattttt tgaggtgtag gatatgaagt
      121 tggattacct tatagaagag gaagatagag aaaaactgct gctagctaga tatttagaat
      181 ttgagaaaaa gaattatgta gtgctgccta agttatatga aacattaggt ttgtctaaat
      241 ttaaaacaag aaattatatc atagaattaa ataaagaatt tactgaattc aaaacaaagc
      301 cagagatcca aatttcagaa gaagcagaga tccatgtcga acgtctgaat ttgacggata
      361 tcaaaaattt aagaagaatc tactttcaac gatcaaaaat tgctcgactg ttaacggaga
      421 tcgtggattc aaaagtaacg atcgaaaaat ttgcagagaa tcatttttta agcatatcaa
      481 gagcctatgt aaaaagaaga gaattagtct cttttcttga acagcatcaa ataaagttaa
      541 agaaaaacat attagttggg gaagaaataa acatacggaa cgtattattt ggggtatatt
      601 tcggggtctt taatggtttt tctatgccat ttagagaaag cgtcatgaat gaagcaaaga
      661 gtatcgtgca atattttgaa tatctgttca atttaaaact gtctgcaaca aaacgaatca
      721 aattcaattt gctaaccact attagtttgt tgagggccta tgggaatcaa ccaatcggaa
      781 aacgtttctt ttcagatgaa tactttaaga gctcagaagg aatgaagatc atcaaggatt
      841 tatcacatat ttcttttctt catatcccta aagagcagat cattgatgag atttcttata
      901 tcatgttgtt tttgatcacg gaagaagaat ttgaattaga tggcgcattt ggacttgcgg
      961 aagctcattt tcatgatatc cacatgatta gtcaaaaaat gtcagtggaa ttgattgatc
     1021 aattaccatt ttcgattgaa atctctgaag aagaaagact atatatacga aaagaacttc
     1081 gtatgggtat gatgcatgtc aatcgaaaaa attggatatt cgactttgag atgactagct
     1141 ttacgtctaa aaagcagcta cagtttttct tcgagacgta tccaatattc agtacagcaa
     1201 tccaaaaggt catacaagag aataaacgct atttcttggg aaaagatgaa cgtttcatga
     1261 ttcgcttatt ttatgattat ctctttttat tggtcggtat ttgtccggta tcatattttg
     1321 aagcaccgat tcatgtctgt attgattttt cgcaaggatc tgcttatacc gattacatta
     1381 tttctcaa
//
LOCUS       DAXWLG010000304         1371 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXWLG010000304 DAXWLG010000000
VERSION     DAXWLG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.312"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..757
                     /locus_tag="ACGE4T_002814"
     CDS             <1..757
                     /locus_tag="ACGE4T_002814"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8388010.1"
                     /translation="KNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAEST
                     TASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKDG
                     YYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQD
                     PNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDV
                     VDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(872..1312)
                     /gene="fabZ"
                     /locus_tag="ACGE4T_002815"
     CDS             complement(872..1312)
                     /gene="fabZ"
                     /locus_tag="ACGE4T_002815"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8388011.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          452 a          269 c          256 g          394 t
ORIGIN      
        1 gaaaaataaa gttattttaa gtattagttt agcagcagca ttgctgtttg ctgctggttg
       61 ttctgcaaac aacacgactt cttctagttc gtctacagaa agttcaaaaa gtacatctac
      121 tgcggaaagc acgactgcca gtagcgtcga tttgaacgca ttggaattac cacaattgac
      181 gaaagatgtc gccgaaaatg aagatctggt cgaaatggtg acgaataaag gcacaatcaa
      241 aatcaaatta tttcccgaac acgcaccaaa agcggttgaa aactttatga cacatgcaaa
      301 agacggttac tacaacggac tgactttcca tcgcgtcatc aaagatttca tgatccaagg
      361 tggcgatcct aaaggcgatg gcactggagg ggaaagcatt tggggagaag gatttgaaac
      421 agagatcaac aaccaccttt ataatataag aggtgcgtta tctatggctc gctcacaaga
      481 tcctaacagt aacggcagcc agttctttat cgttcaaaat acagatgata tgcatgatgg
      541 tcttttaaaa gacgactatc ctcaagcaat catcgacgct tataaaaacg gcggttatcc
      601 ttcactagat ggccattata ctgtcttcgg ccaagtgatt gaaggaatgg atgtcgtgga
      661 tgctattgca aatatcgaaa cagatagttc agacaaacca aaagaagaag taaaaatcga
      721 taaaatcaat atactgcaag aagcaaaaca aaaataaaaa aataacgtct gaatcctgca
      781 tagatgacaa cgtatgctag gattcaggcg tcttcttttt acataaaaag caagtaagct
      841 tccttttgtt ttgctatatc aggttttata gtcaagcatt ttggttgacg atgaacgtga
      901 actcgcactc acagactttt ttatcatcta catatgctgc agataacgct gtaccgactg
      961 atcccttcat tttggtgatt tcaaaatgac atttcagcac atctcctgga acaacttttt
     1021 gacggaattt ggccttattg atcccaccga tataagctct ttttcctaga aactcttctg
     1081 attttaggat aagtatcgaa cctgcttgtg ccagtgtttc tagaatcaac gcacccggca
     1141 tcactggatt gtcaggaaag tgaccttgga aaaaatcttc attgattgtt acattttttg
     1201 tcgcaacgat cttttttcct ggttctaaag tatctacata atcaatataa caaataggat
     1261 aacgattagg aatcaattcc attacttcag tagcagataa tacgtaagac ataattaaac
     1321 tccttctttc aggcaattgc cattttcatt actttgacta tcaaagtatc a
//
LOCUS       DAXWLG010000305         1370 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXWLG010000305 DAXWLG010000000
VERSION     DAXWLG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.313"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..873
                     /locus_tag="ACGE4T_002816"
     CDS             <1..873
                     /locus_tag="ACGE4T_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFF8388012.1"
                     /translation="NFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRIL
                     VNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKIL
                     YSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLL
                     PVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESI
                     KIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEE
                     ALMDQSKKENQVTS"
     gene            complement(989..>1370)
                     /locus_tag="ACGE4T_002817"
     CDS             complement(989..>1370)
                     /locus_tag="ACGE4T_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8388013.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          467 a          226 c          227 g          450 t
ORIGIN      
        1 aattttcctg caacttctgt gaaagacgta caaaaagaat tcacagagta tgaatatatt
       61 gactttttta ttgatccctc tgtagatatt ttgcgaccta atctgccaat ttgtcgcgaa
      121 caacgcatct tagtgaatat tttagctcga ctttctatca cgaaaatcga atcggaagaa
      181 acaagcttgg agcaatacaa acttttaaaa aaagcaaacg ttcctcatgt tctttattca
      241 agagaaatgg tggagtcctt ttgtactgta tttgatttgt ttatacctga aaaagaaaag
      301 attctttact cattggcatt cgcacgaaat aaactttata atggatttct taacttcaat
      361 caaccaaata cgccccttcc tactttcatg ctttatcaag aacatagcgt gttgaaagaa
      421 gtccaaaccg ccattgaaca attttatatg gatttcagag aacaaaatca acacttgtta
      481 ccagttattt tagaaaagga aaatgtgcaa tggatggttg aagacctagt acatttgtat
      541 gatcgctaca aaaaacagcc gcgaattgtg attggtgtga actatacgcg tgatttttat
      601 atcagtaaag atctgatcgt taagatagaa caaatttttt caaatgaatc gatcaaaata
      661 caaaatgatt ttatggagaa ctgcaacatc gttatcagcg actgcccttt gaaacaattg
      721 ccttctcata tcaaaaaaat ctatatttta aaaggaatca tcacaccaga agactggaaa
      781 aaagtgatta tgaggatctc agaatatatc tttgaattaa aagaagaagc actcatggat
      841 caatcgaaaa aagaaaatca agttacttcc tagaccaaat aagatacata aatccgaatg
      901 cttattaaca acctccggat ttatgtatca agaattggga gcgtcaataa ttttgtgtaa
      961 ataaattgtc ctcctgcaaa ataattagtt actcagtaaa cattgaaact aatgtatcgg
     1021 ttacctgttg aaaaccttta tggcttctgt ttagaaattt ttgattgtat gtatcaaaaa
     1081 tgctgactag aaagcgttct agtgattctt cattttgaaa ctgctctttt ctacggctgt
     1141 atcttttaat ttgcttattg aaagactcga ttagattggt tgagtaaatg gttctacgaa
     1201 tgctaggtgg aaaatcataa aaagttaata agtcttggtt ttctatgagt gactgcgtca
     1261 ctttaggata gtttttcttc catttctcaa tcatgccgga taagaaggta ttcgcttctt
     1321 cttttgagtt agcttgataa acagccttaa agtcatcaca gatttctttt
//
LOCUS       DAXWLG010000306         1370 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXWLG010000306 DAXWLG010000000
VERSION     DAXWLG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.314"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            434..>1370
                     /gene="bioB"
                     /locus_tag="ACGE4T_002818"
     CDS             434..>1370
                     /gene="bioB"
                     /locus_tag="ACGE4T_002818"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010709179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFF8388014.1"
                     /translation="MMNTINTKMTKSDCLEILSSKENIWFYYSQALYTKTKNMKNRIR
                     LNTLLNAKSGLCNEDCGYCAQSKKSKATIQKYSLLPQDEIIRKALIAKKNNSSVFCIA
                     LSGTRPTQKEVLVLGETIKEIKRIMDIEICLSIGLITDNQIRYLKKCGIDRLNHNLNT
                     SRENYPNITTTHSYDDRLNTLKKIKAFDINICSGFICGMGETDTQLVDLAFELKEQEP
                     FSIPVNFLLPIKGTKLEKYNELTPIKCLKILIMLRLLFPKTEIRISAGREFHLGSLQQ
                     FAILIVDSIFLGNYLTEKGADVTEDYQLLKELELIV"
BASE COUNT          538 a          188 c          188 g          456 t
ORIGIN      
        1 cctttcttta ctagtaaaaa atattttttc ttccttaaat ttccaaaatt attctaacag
       61 caaatgtact tcgttttaag tatgctttat taaatactat ttgcctttta aaatagcgga
      121 taaatatgat taattaggga gaaaaaggga tatattcatc tcctagaact cttaactaaa
      181 tggcctttta gtaggtagca attgataaaa actaatatat tgaatgctag taactcttga
      241 taaagataaa actaacaaaa tttttttaat taaagaatta gcagctttcc aaatgttttc
      301 atttaactat gtccctaaac tgataagctt atcctttatt aataaaattt aaacattatc
      361 agtaatttac cccttcattt tcgttaacct gtataatata attggttaac caactacaag
      421 gagaattact atcatgatga atactattaa tacaaaaatg acaaaatctg actgtttaga
      481 aattttaagt tcaaaagaaa atatttggtt ttactattcg caagccctat atactaaaac
      541 taaaaatatg aaaaatagaa taaggttaaa tacacttcta aatgctaaaa gtggattatg
      601 taatgaagat tgtggttact gcgcccaatc taaaaaaagt aaggcgacca ttcaaaaata
      661 cagtctttta ccccaagatg agattatacg taaagcattg atagcaaaaa agaataattc
      721 aagtgttttt tgtattgcgt taagtggaac tagacctact caaaaagaag tattagttct
      781 cggcgaaaca attaaagaaa ttaagcgcat tatggatatc gagatttgtt tatctattgg
      841 attaatcaca gataatcaga taagatactt aaaaaaatgc ggaattgata gattaaatca
      901 taatttaaat actagtaggg aaaactatcc taatataact actactcatt cctatgacga
      961 tcgtttaaat acgttaaaaa aaatcaaagc ttttgacata aatatctgct ctggctttat
     1021 atgtggaatg ggagaaactg atacacaatt agtcgattta gcttttgaat taaaagaaca
     1081 agagcctttt tcaattcctg taaatttttt attacccatt aaaggaacca aacttgaaaa
     1141 atataatgaa ctaaccccaa taaaatgtct aaaaattcta ataatgctta gactattgtt
     1201 tccaaaaaca gaaattagaa taagtgctgg tagagaattt catttaggca gtcttcagca
     1261 atttgctatt ttaattgtag attctatatt tttaggaaac tatttaacag aaaaaggcgc
     1321 ggatgtcaca gaagattatc aattattaaa agagttagaa cttatcgtag
//
LOCUS       DAXWLG010000307         1364 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXWLG010000307 DAXWLG010000000
VERSION     DAXWLG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.315"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            82..>1364
                     /locus_tag="ACGE4T_002819"
     CDS             82..>1364
                     /locus_tag="ACGE4T_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFF8388015.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          463 a          255 c          259 g          387 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAXWLG010000308         1354 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXWLG010000308 DAXWLG010000000
VERSION     DAXWLG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.316"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            135..1007
                     /gene="scrK"
                     /locus_tag="ACGE4T_002820"
     CDS             135..1007
                     /gene="scrK"
                     /locus_tag="ACGE4T_002820"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFF8388016.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          428 a          235 c          311 g          380 t
ORIGIN      
        1 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
       61 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
      121 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
      181 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
      241 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
      301 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
      361 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
      421 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
      481 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
      541 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
      601 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
      661 actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac
      721 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
      781 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
      841 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
      901 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
      961 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     1021 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     1081 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     1141 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     1201 tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat aggttctgtt
     1261 gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata gcaggaggaa
     1321 tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAXWLG010000309         1352 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXWLG010000309 DAXWLG010000000
VERSION     DAXWLG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.317"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(48..848)
                     /locus_tag="ACGE4T_002821"
     CDS             complement(48..848)
                     /locus_tag="ACGE4T_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFF8388017.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENLTDSLLKTLQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESSEELKAKYGEDFEGIRKIY"
     gene            complement(1123..1272)
                     /locus_tag="ACGE4T_002822"
     CDS             complement(1123..1272)
                     /locus_tag="ACGE4T_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388018.1"
                     /translation="MKKKVIPYCPKPKNAFERGLFKYFEGKKGQDHKEENQKKKSKKK
                     SEEFF"
BASE COUNT          412 a          248 c          168 g          524 t
ORIGIN      
        1 acaattagtg tcagcaaaat attccgaatc ctaaaaattt cctcctacta gtaaatcttt
       61 ctgattccct caaagtcctc accatatttt gctttaagtt cttcagaaga ttctgcaatc
      121 acttttagtg tatatgcatc atgtttgaca tattcttttg ctagattgac catggcgtgg
      181 ttttttgtcc aagtgacatc ggttgattgc agttcttcgt taatggtgcc aaacacgact
      241 tcttgctcat cggcgacgat gtttatccat cttgctccaa agtcagcaag tttgtctgtt
      301 tcaaagccat gaatataata tttttctaag ggaagatcat attcatgggt agaactgaat
      361 aaaattagta caaacttatc aactcttttt tcagcttgct gcaatgtctt tagcagtgaa
      421 tccgtgaggt tctcatgcca aacttgaatg agcaaacttt cttttgcgtt tttcaccaaa
      481 tgctctactt tatccagaac atactcgatg ccatcaactt tccacagcat ttcttcttca
      541 tctttttctt tgatcatccc tagattcttg gtaagaaaag aaagatcatt tttaacactt
      601 ttttctaatt tctctaaaaa ctcatttgcc gagatggcat gataaagttt aggttctgat
      661 ttattaacga caatcaaatt cttttttaat agtttttcca aaatgttata tactttcgag
      721 cgtggcacac ctgagatttt acttacttca tatcctgtaa gattaccttt ttctaatagt
      781 gtggtataca ctcttgtttc catctctgaa aaatcatatt ttttcatgat ttgaataatc
      841 gtttccattg attaatcctc actttatttt tagtagctac tacggtaaca actataaatt
      901 ctttttgatg atctgtcaag agttagttca ataaaacaca tataataatt agcttaaaaa
      961 caagcttttt attaaaaaga aacattataa tataaatctt tgtgaaaaaa taatacaact
     1021 tagtcattct ttacaacgat ttacagaaaa acgacctctt taaaaataaa aggtacgggt
     1081 tactgattac ttgtaaatct ttacgttcca gttttattct ttctaaaaaa actcctcact
     1141 ttttttctta gacttctttt tttgattctc ctctttatgg tcttgtcctt ttttgccttc
     1201 aaaatattta aacaaacctc tttcaaaagc atttttaggt tttggacaat aaggaataac
     1261 ttttttcttc atataaacac ctcatttaac tatttgatct attttatatc accacattat
     1321 caaaataaca aatgtattaa aagaaagttt tt
//
LOCUS       DAXWLG010000310         1346 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXWLG010000310 DAXWLG010000000
VERSION     DAXWLG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.318"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(158..1299)
                     /locus_tag="ACGE4T_002823"
     CDS             complement(join(158..1071,1071..1299))
                     /locus_tag="ACGE4T_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8388019.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          331 a          270 c          284 g          461 t
ORIGIN      
        1 cggacacgat ttgtgagaat tttttcgaga ttccagcggg tgagtatctc atcgtttcga
       61 ccagcggtca aaccaaaacc gtttgaccgc tgatcgaagc gatgaagttg aaaccctgtg
      121 gaaatctcgc caaaaaaatg taagtggtag ctaggaacta agcttgcaat gccaactcct
      181 ccatctgatt tggtgttaaa tagttgatac tactatggat tcgcttgcga ttgtaaaatc
      241 cttcaatgta ttgaaataac gctaaataag cagcttcaaa gttttcatac gcttttgatg
      301 gatagacttc ttcttttttg agtgaggcgt gaaacgattc gataccggca ttatcgtagg
      361 gacagccctt acgactgaag gagtgccgaa tgttcaattc attcagcttt gcctcatagg
      421 cttcgctagt gtactgagaa cccaaatctg agtggatgat caagttctcg gagggtgttt
      481 ggtttaagag cgcattgtca agggtttcga taaccaattc tttcgtcatc tgcttgccaa
      541 acttccaacc gatgactttc tttgtgtgga ggtcttgaat agtggagagg taggtccagc
      601 catcttttag ggtatgaata taggtaatat ctgtcaccca tttttgattg atcgacgtag
      661 tggtaaaatc ttggtttaac aggtttttac gtccttcact tttaactttc gcttgtttgc
      721 caggcttgta cttcttgagg gtgactgaac gcaagcctaa ttttctcatc agtatttgta
      781 ctttctttaa ggacaaggtc tcgccacggc caacaagaat cttatgaatt ttaggcgcac
      841 catagatacc cttgttttca aagtagattc ctgaaatcaa gcggtttaag cgtttgtttt
      901 ctgattggcg cttggaaggt ttctttttct tccagtcata gtagctggat ttcggtgctt
      961 ccaaaacggt acacatttgt tttacgtcat gatcatgaac atttgattga ataaattcga
     1021 agacatcact catttttact tttgagcgaa tatggttaaa gcctttttta ggatagtgtt
     1081 ttcctcctgc atgcgggcca tttccttttt catcgctagg atttctgcct cagtcatgcc
     1141 ggttgctaca ttctttgtat gaagcttctt ccaacgataa atcgtttgtg tagcgatacc
     1201 atattgcgct gccagttctt cgaccgattg gccagactgg tttaaatcta agatcatctt
     1261 cttaaagtct tcatcaaatt ttttcttggc ttttgccatc gtggacacgt ccttttctta
     1321 gtagttagat tctaccaaat cgtgtc
//
LOCUS       DAXWLG010000311         1282 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXWLG010000311 DAXWLG010000000
VERSION     DAXWLG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.319"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            35..532
                     /locus_tag="ACGE4T_002824"
     CDS             35..532
                     /locus_tag="ACGE4T_002824"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8388020.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(515..1252)
                     /locus_tag="ACGE4T_002825"
     CDS             complement(515..1252)
                     /locus_tag="ACGE4T_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFF8388021.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKG
                     KRIMVPLPVQKNVLSIKSEDVFLDFFF"
BASE COUNT          446 a          217 c          225 g          394 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt ggactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt aattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgacc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttcgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaaatag tc
//
LOCUS       DAXWLG010000312         1279 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXWLG010000312 DAXWLG010000000
VERSION     DAXWLG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.320"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            24..1190
                     /locus_tag="ACGE4T_002826"
     CDS             24..1190
                     /locus_tag="ACGE4T_002826"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8388022.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT          459 a          217 c          244 g          359 t
ORIGIN      
        1 tagttttttt aaggaggaaa atcatgttag agaaaacttt atacaatcaa ttattttcac
       61 gttctttctc gctccctgtc gaagtaaatt attgggacgg tacaacgaaa caatacggag
      121 atacggacac tcctccccaa atcaaaatca atatccatga agaaatccca atgaaagaga
      181 ttacaagtaa cgcttctttg gcactaggag aagcctatat ggatcatcga atcgagatcg
      241 agggaagtat ccaagcattg atcaacgatg cttatcagca agcaaacagc tttttacgag
      301 gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag
      361 aagatatcca tgcgcattat gacttaggta acgactttta taaactttgg ctagatccaa
      421 ctttgactta ttcttgtgct tatttcaata cacctgaaga tacgttggaa caagcacaaa
      481 tcaataaagt ccatcatatc ttagacaaac tctttatcaa agaaggcgaa acgttgcttg
      541 atatcggttg tggctgggga acattgatgt tcactgctac caaagagtat aatgtcaagg
      601 caactgggat cacactaagt gaagaacaat acgatttgat taccaaaaga ataaaagaag
      661 aacatttaga agataaatgc cgtgtattat taatggatta ccgcgaacta aaaggcgaaa
      721 cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag
      781 gttatttcaa agtggtaaaa gatttattga aaccaaaagg aacagctttg atccatggaa
      841 tcagccgaca acaaggcggt gcgacaaatg cttggatcaa taaatatatt ttccctggcg
      901 gttatgtccc tggtgttact gaattagttg ataatattac aaaaaacgat ttgcaactga
      961 ttgatttgga aagcttacgt cgtgactacc aattgacatt agaacattgg acaaagaatt
     1021 ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga ttccttctat cgaatgtggg
     1081 atctatattt acaagcttgt gcagcttcat ttgaatcaag taatatcgat gtgattcaat
     1141 atttgcttgt tcaacctgga aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa
     1201 agtactgttt aaagactatt taaaaagaag ctaagtttta tgatgctcca gaagtgaact
     1261 tttttgtcta acttctagg
//
LOCUS       DAXWLG010000313         1263 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXWLG010000313 DAXWLG010000000
VERSION     DAXWLG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.321"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            162..1202
                     /locus_tag="ACGE4T_002827"
     CDS             162..1202
                     /locus_tag="ACGE4T_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8388023.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          508 a          179 c          208 g          368 t
ORIGIN      
        1 gactttaaag gtagtacata attcatttta tttctgattt taggtagtat ttaattaaaa
       61 aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa agagtaggta
      121 attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat tttaaatttt
      181 ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc tttattgttt
      241 ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa
      301 gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt tattttactt
      361 atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg atagcaatca
      421 aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt aacaaacagt
      481 tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca gaaaatgata
      541 tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa tcagagggta
      601 tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg gctcaagagg
      661 gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa atcataccag
      721 aacttaataa taatatatta gacactaata gacacaatat agacactgaa aaagaccgcc
      781 tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac agcattgcta
      841 cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt tacagtgaag
      901 ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata gaaagtgata
      961 tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa gaattataca
     1021 cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc
     1081 tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct aaagtatcaa
     1141 gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac tggttagagt
     1201 gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt aaaaattttt
     1261 tat
//
LOCUS       DAXWLG010000314         1260 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXWLG010000314 DAXWLG010000000
VERSION     DAXWLG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.322"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..1223
                     /locus_tag="ACGE4T_002828"
     CDS             <1..1223
                     /locus_tag="ACGE4T_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8388024.1"
                     /translation="IRSSTKKEPIIPEKKITEKAKEPTDDKQQLVTQAKNLAQSYDYD
                     QAIALLEKDSKNGSTETQQLLQTLKKEKEQLIDWSDPTQISHVFFHSLIVDPQKAFHS
                     QQAQGYKDYMVTVEEFNRSIEQLYQNDFVLVNLNDLIQKDEQGNLSFNGLALPEGKKP
                     LVLSQDDVSYYEYMDNSGFPSRLVVSNNNEIKNVYKEKDKEEIGDFDMVPLIDAFVKK
                     HPDFSYRGAKGTLALTGYNGVLGYRTSKSQYGDNEKTKKEIKEAKKVAEQLKKDGWSF
                     ASHTWGHLNMTQASLEDVKKDNELWQQEVAPILGKTNILIYPFGADISDWQPYSAENQ
                     KFAYLKSQGFDIFCNVDASTPAWGQLGTDFYRNARINIDGIRFQSDLDGKNPILDPFI
                     NVHDVYDKEARESA"
BASE COUNT          456 a          237 c          236 g          331 t
ORIGIN      
        1 tcatccgatc atctacaaaa aaagaaccaa tcattcctga aaaaaaaata acagaaaaag
       61 caaaagaacc gacagatgat aagcaacaac tagttactca agcaaaaaat ctggctcaaa
      121 gctacgatta tgaccaagcg attgcgttat tggaaaaaga tagtaagaac ggatctactg
      181 aaacacaaca gcttcttcaa acactaaaaa aggagaaaga acaactgatc gactggtctg
      241 atcctactca gatctctcat gtctttttcc acagcttgat cgtggacccg caaaaagcct
      301 tccactctca acaggcacaa ggatacaaag attatatggt gactgttgaa gaattcaacc
      361 gttcgatcga acaactttat caaaatgatt ttgttttagt taatttgaat gatttgatcc
      421 aaaaagacga acaaggaaat ctttccttta acgggcttgc gctacctgaa ggaaaaaaac
      481 cattggttct ttcacaagat gatgtcagct attatgaata tatggataat tccggttttc
      541 ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa gagaaagata
      601 aagaagaaat cggtgacttt gacatggttc ctctgatcga tgcctttgtc aagaaacatc
      661 ctgatttttc ttatcgaggc gcaaaaggta ccttggccct gacaggttac aacggagttt
      721 taggttatcg aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa aaagagatta
      781 aagaagctaa aaaagtggcg gaacagttga agaaagacgg ctggagcttt gcttcccata
      841 catggggtca cttgaatatg actcaagctt cactagaaga tgtaaaaaaa gacaatgaat
      901 tgtggcagca agaagttgca cctatcctag gaaaaacaaa tattctgatt tatccatttg
      961 gtgcagacat cagcgactgg cagccttatt cagcagaaaa tcaaaaattt gcttacttga
     1021 agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc tacaccagct tggggacaat
     1081 taggtacgga tttctatcga aatgctcgga tcaatatcga tggcattcgt tttcaatctg
     1141 atttagacgg aaaaaatcct attcttgatc cattcatcaa tgtccatgat gtctatgaca
     1201 aagaagcacg cgaatctgct tgattttacg tcatttatga accaaaaaag ccgaactgac
//
LOCUS       DAXWLG010000315         1249 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXWLG010000315 DAXWLG010000000
VERSION     DAXWLG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.323"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>1249
                     /locus_tag="ACGE4T_002829"
     CDS             <1..>1249
                     /locus_tag="ACGE4T_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388025.1"
                     /translation="NRFYETYMQIKTGNFNFFQSLYSFQSSGRIITAFYGADFAYLQG
                     LLLIILKSWFKYQLVSSFSCFFIAGASMFWLTYKCKVRTNIGLIIALLYMSSSAVSYY
                     PIAQAFTGWGAALMPLLFVPAIEALKNKERPIRPLQLAIPVTLLLSTHLLSLVIGILA
                     ILPFYVIAFINTKNKLNMIFRLVESIGLTMLFSANTIIGFIDVYLSNKILSPSPISQM
                     LSQSMSFSLEGNSWGNYGLVFTAIFFAVIIYFFLNWAKTSLTSKVIVIVGAFFMLLSS
                     KLLPWNSIPHMFKFVSFFQFPQRFSVIAFVLLLLSFALILQESKLLKDVDKKYYILTL
                     LCALFSIFNVYNLMYDQSWHWNTNDPTAAGNNKSSMVEKDPQKLREAFYNKDLNIALK
                     AIQKGTPDYLPVQKNVEPSDVLK"
BASE COUNT          382 a          202 c          181 g          484 t
ORIGIN      
        1 ttaataggtt ttatgaaact tatatgcaga taaaaaccgg taattttaat ttctttcaat
       61 ccctgtattc ttttcagagt tctggcagaa ttattactgc tttttatggc gcagatttcg
      121 cttatttgca agggctactt ttaataatct taaaatcatg gtttaaatat caacttgtct
      181 cttctttcag ttgctttttt atagctggtg cttcgatgtt ttggctaact tataagtgta
      241 aagtacgaac taacatagga ttaatcattg ctttgttgta catgtcttca tcagcagttt
      301 cgtattatcc tattgctcaa gcttttactg gttggggtgc tgctttaatg ccgcttcttt
      361 ttgtgccagc aattgaagca ttaaaaaata aagaaagacc tattcgtcca ttgcaacttg
      421 caatccctgt aactttatta ctatctactc atttactttc attagtaatt ggaatattag
      481 ctattttacc tttttacgtt attgctttta ttaatacaaa aaataaactg aacatgattt
      541 ttcgcttagt agaatctata gggttaacca tgctattttc tgctaataca ataattggat
      601 ttatagatgt ctatctaagt aataaaatat tatccccttc tccgataagt caaatgttga
      661 gccaaagtat gagtttctcg ttggaaggaa actcttgggg aaattatggt ctagtattta
      721 ctgcaatttt ctttgctgta ataatatatt tctttttaaa ttgggctaaa actagtctta
      781 cttcaaaagt aatcgttatt gttggagcgt tttttatgtt actttcttcc aaattattac
      841 cttggaactc tatccctcat atgtttaaat ttgtttcatt tttccaattt ccacaaagat
      901 tctcagtcat tgcttttgtt ttacttttat tatcatttgc tcttatattg caagaatcaa
      961 aactacttaa agatgttgac aaaaagtact atatattaac tttactgtgt gctctatttt
     1021 caatatttaa tgtatataat cttatgtatg accaatcttg gcactggaat acaaatgatc
     1081 ccaccgctgc aggaaacaat aaaagttcta tggttgaaaa agatcctcaa aagttaaggg
     1141 aagcctttta taataaagat ctgaatattg ccttgaaagc aatccaaaaa ggaactcctg
     1201 actatttacc tgtacaaaaa aatgtagaac catcagatgt attaaaaca
//
LOCUS       DAXWLG010000316         1238 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXWLG010000316 DAXWLG010000000
VERSION     DAXWLG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.324"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            278..1189
                     /locus_tag="ACGE4T_002830"
     CDS             278..1189
                     /locus_tag="ACGE4T_002830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8388026.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          491 a          186 c          243 g          318 t
ORIGIN      
        1 agaagaaaaa caaaatcgat aacatttgtt ctaagaatta gttacgttaa atacggaacg
       61 caagatagac ttgaaaaacg gtaacaaaag aagtagtata ttaaggtgtg atgtgctact
      121 tgacatttat tctgctgagc gaactttctg gattgtgtag ccttatatct attcaggacg
      181 tatcgagcag attagtaaag gtgttcttga tgattttcat cttgaacacc tttgttttta
      241 gcaattcgca aaaaacgaaa agtaggagaa ttgataaatg aaaaaaagat ttttagcact
      301 tgccatcgta ctaggcactg gcttattgtc aggctgtacg aatgccggtg aaaaaacagc
      361 cgttagttat aaaggcggta caatcagcga acaagaagta atggatagct tgaaaaaaat
      421 gcaaggagca gattccgctg tgcaacagtt gatcgtttat caagtatttg aagataaata
      481 tggagatgac gtatcaacca aagaaatcga ttctcaatat gatcaaacaa agaaacaatt
      541 gggtgattca tttgattcac aattgaaatc tgcaggttac acagaacaaa cattcaaaga
      601 cagtatcaaa caaagtctag cctttcaaga aggtctaaag aaacatatca aattgaccga
      661 tgaagattta aaaacggctt gggaatcttt ccatccagaa gtggaagctc aaattatcca
      721 agtagcaagt gaagatgatg caaaagatgt gaagaaagct gctgataagg gagacgattt
      781 ctctaaatta gccaaagaca agtcaactga tactacgaca aaagaagatg gcggaaaagt
      841 gaaatttgat tccactacta caacggttcc ggcagaagtc aaagaagcag cctttaagtt
      901 gaaagatggt caagtttctg atgtgatcac ttctaccaat gcttcaactt atactacgga
      961 atattatgtg gttaaaatgg tgaaaaacca aaacaaaggc aacgacatgg ataaatacaa
     1021 aaaagaattg aaagaaattg caacagacac aaaattaagt gacagtacct tccagaataa
     1081 agtaattggc gaagtattga aagacgctaa tgtaaaaatc aaagacaaag actttgaaaa
     1141 tgttttgagt acattcactt cagattctag tacagcaagt agtaaataag cggtataaat
     1201 aaaaaatatg aactatttaa atcaataaaa ctgttcat
//
LOCUS       DAXWLG010000317         1235 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXWLG010000317 DAXWLG010000000
VERSION     DAXWLG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.325"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(65..1153)
                     /locus_tag="ACGE4T_002831"
     CDS             complement(65..1153)
                     /locus_tag="ACGE4T_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8388027.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT          410 a          233 c          207 g          385 t
ORIGIN      
        1 gccacgttaa aatggtatca caaagcaatt ttctttgcaa taaaaacaag tgtactaaaa
       61 ctacctacga ggtcgtagca attttcttct tgttttcatt ttgcttgtct tctttgacga
      121 ttttgattat ataggaaatg atcgtcttgc agattgcgta aaaaggaaca cctaagaaga
      181 taccaagcaa tccggcaata tttccagcta ctagaagaac gataatgatc gttaatggat
      241 ggattttcag tgatttccca attacattag gataaatgat attcccatcc aactgttgga
      301 cgattagcac aaccacacaa caaagtaatg cttttaccgg atcattgaag actgttacta
      361 gaaatgcagg tgctagtcct agataaggtc ctaaatatgg aattaggttt gtcaatcccg
      421 caatgacccc aaataagaaa gcataatcca caccaattac catatatcct aaaaatgtga
      481 aggtaccaac gaataaacat tcgattgctt gtccgctaat gtaattagcc aatgttttat
      541 tcagttgccc caataactcc acaatttggt ttcttctttt ttctgggaaa aactgttgga
      601 tgtttggcac tagtttttct ccgtctttca acatatagaa caagatgaat ggtgctgtaa
      661 tgattactac ggttgccgaa gcgatcgttc caacgatcga acctaaacta ttggatactc
      721 cactaaggaa ttgctgaatg attgtcccgt aagaaatgtc taattgctca aaatatttat
      781 tgatatctac ttccttgaac aaaggcaatt cggctacctc ttttaaccag ctttctacct
      841 gtttgatgaa atcaggcata ttagatgcca atgaggaaat ttgctgtact agacttggga
      901 tgacactaag taaaatccaa ataattgctg caactaaaag gagcagcaca attgcgacag
      961 cccatattct tttgattttt gctttcatca acagcttgac aataggattc aataaatagt
     1021 aaagaaagcc tgcaatcagt acaggtgcaa ataacgtgga aaaaaatgtc ccgatcggtt
     1081 cgaaaataaa gtttatttct gatgaaatca tgatcaatgc agctatgatc aataattcaa
     1141 gcgaccaaaa cattagttta gactgtctaa gtttttcaaa caatagattt cctcctttct
     1201 ttcttactag tatatattat attcgttaaa aattc
//
LOCUS       DAXWLG010000318         1210 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXWLG010000318 DAXWLG010000000
VERSION     DAXWLG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.326"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(4..195)
                     /locus_tag="ACGE4T_002832"
     CDS             complement(4..195)
                     /locus_tag="ACGE4T_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8388028.1"
                     /translation="MNTLKDDFILAKAGSEEAIESILKRFSSLMHQQSWRNGRYDQDC
                     YQECMIAVYLAISKFEIKE"
     gene            complement(269..655)
                     /locus_tag="ACGE4T_002833"
     CDS             complement(269..655)
                     /locus_tag="ACGE4T_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8388029.1"
                     /translation="MSENDERVNAQLICFFEKVIKNTAYSYFERQKKIRELEQFDQFS
                     QHFFTENNIKIKQPVTILDVTFLIEDSRLAEIIPLLKEREQLFLIEKFVFDKTDKEIG
                     ESFGITRQGITNLKHRLYRKIQKLLI"
     gene            complement(716..1177)
                     /locus_tag="ACGE4T_002834"
     CDS             complement(716..1177)
                     /locus_tag="ACGE4T_002834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388030.1"
                     /translation="MKLEEEKDAIYTQMRMILEERRRLSKDYYELKARLFALDNTNQP
                     NSLIRNEKLAKKSSTNISEEALYQQYICERGNKYRQLIPYSTISLTISSILKDAGRPL
                     SNKEIYKLLISDHKLNISYKNLTHNILPKMNVDNSVNVERAYRGYWQYRLH"
BASE COUNT          377 a          195 c          168 g          470 t
ORIGIN      
        1 attttattct ttgatttcaa attttgatat tgcaagatat actgcaatca tacattcttg
       61 atagcaatct tgatcatatc ttccatttcg ccatgattgt tgatgcatca aagaactaaa
      121 tcttttaaga atagactcaa tagcttcttc acttccagct tttgccaaaa tgaagtcatc
      181 tttcaaagta ttcatagacc gtcctccttt cctaaattta aagttattta gaggaggtgg
      241 tcgtatacaa ataatcgtta agtggctttt aaataagtaa cttttgaatt tttctgtaaa
      301 gacgatgttt taagtttgtg atgccttgtc tagtgatccc aaaagattct ccgatttcct
      361 tatctgtttt atcgaaaaca aatttttcaa taagaaatag ctgttctctt tcttttaata
      421 aaggaataat ctctgccaat cttgaatctt caattagaaa agtaacgtct aaaatagtaa
      481 ctggttgttt gattttgata ttgttttcag taaaaaaatg ttgagaaaat tgatcgaatt
      541 gttccagttc acgaatcttt ttttgccttt caaaataaga gtacgcagta tttttaataa
      601 ccttttcaaa aaaacaaata agctgtgcat tcacgcgttc atcattttct gacattctgc
      661 tcacttcttc caataaatat ttaaaaggaa aagggacaga aagcgaaaaa agaagctaat
      721 gcaatctata ctgccaataa cctctataag ctctttctac attgacacta ttgtcaacgt
      781 tcattttggg tagaatatta tgagttaagt ttttgtaaga aatattaagt ttgtgatcgc
      841 ttattaatag tttatatatt tctttatttg ataatggtcg accagcatct tttagaattg
      901 aggaaattgt caaactgata gttgagtatg gaatcaattg tctatattta tttcctcgct
      961 cgcaaatata ttgctgatac agcgcttctt cagaaatatt tgttgacgat tttttggcca
     1021 atttttcatt tctaattaaa ctattaggtt gattagtatt atctaatgca aaaagtctgg
     1081 cctttagctc ataataatct ttactaagtc tcctcctttc ttcgagtatc attctcattt
     1141 gtgtataaat tgcatctttt tcttcttcta gtttcatcta cttctccttt catgattaac
     1201 aactaaaaat
//
LOCUS       DAXWLG010000319         1201 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXWLG010000319 DAXWLG010000000
VERSION     DAXWLG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.327"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..339
                     /gene="amaP"
                     /locus_tag="ACGE4T_002835"
     CDS             <1..339
                     /gene="amaP"
                     /locus_tag="ACGE4T_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8388031.1"
                     /translation="YPKANNYIEKKTASGKIAIQKKAIENFVLIAAKEEPFIVDPTVK
                     SVIKKKKIKIYIRGKIRKAFQTQERQTAFIRKVNTNLNELFGAEQTILTEIIFKDYHE
                     KNTNTKSKVL"
     gene            349..531
                     /locus_tag="ACGE4T_002836"
     CDS             349..531
                     /locus_tag="ACGE4T_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8388032.1"
                     /translation="MAEFISKYKYPFIFGCLGLLLGILIFTLGFIKTVILTVLTICGF
                     YLGNYLEIVGFFDKQN"
     gene            587..1165
                     /locus_tag="ACGE4T_002837"
     CDS             587..1165
                     /locus_tag="ACGE4T_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8388033.1"
                     /translation="MENKTNNTKTEIKKNDVAKTSEIIKGELVFEDKVIQKIIGIALD
                     EIDGLLTIDGGFFSNIAGKLVNTDNTTSGVDVEVGKKQVAVDLSIVAEYGKDVTTIYD
                     KIKEVISKEIKKMTGLDVIEVNVDVVDVKTKEQHENDSVTLQDHLSDAASTTGEFASK
                     QFEKSKEALGVASEKVSDGVENVKEASEPRVE"
BASE COUNT          503 a          139 c          211 g          348 t
ORIGIN      
        1 tatcctaaag cgaataatta tatagaaaaa aagacagcta gtggaaaaat agcaatccaa
       61 aaaaaagcaa ttgaaaattt tgtattaatt gctgctaaag aagaaccttt tattgttgat
      121 ccaacagtaa aatctgtaat taagaaaaag aaaatcaaaa tctacatacg aggtaaaata
      181 cgtaaagctt ttcaaacgca agagcgccaa acagcattta taagaaaagt gaatactaat
      241 ttgaatgaac tatttggtgc agaacaaaca atattaaccg aaataatttt caaagattat
      301 catgaaaaaa atacaaatac taaatcaaaa gttttatagg agaaagcgat ggcagaattc
      361 atatcaaaat ataaataccc tttcattttt ggatgtttgg gactattgtt aggaatattg
      421 atttttacgt taggatttat taaaacagta atattaacag tgctgactat ttgtggtttt
      481 tatttaggta actatttaga aattgttggt ttttttgaca agcaaaatta gttaataacc
      541 gtttttacgg taagaaataa atttctaatt tttaggagga aaaataatgg agaacaaaac
      601 aaataataca aaaacagaaa tcaaaaagaa tgacgtggca aaaacttccg agataattaa
      661 aggagaacta gtttttgaag ataaagtaat tcaaaaaata attggtattg cattagatga
      721 gattgatgga cttttaacga ttgatggggg cttcttctca aatatagctg gaaaattagt
      781 aaatacagat aacacaactt ctggagttga tgttgaagta ggaaaaaaac aagtcgcagt
      841 agatctttca atagtggctg aatatggcaa agatgtaact acaatttatg ataaaataaa
      901 ggaagttatc tcaaaagaaa ttaagaaaat gactggctta gatgtaattg aggttaatgt
      961 agacgtcgta gatgtaaaaa cgaaagagca acatgaaaac gattcagtta cactacaaga
     1021 tcatctttcc gatgcagctt ctactactgg agaatttgct tcaaaacaat ttgaaaaatc
     1081 gaaagaagct ttaggagttg caagtgaaaa ggtaagtgac ggtgtagaga acgtaaaaga
     1141 agcatctgaa cctcgcgtag aataacaaat cttttttatt taatgtacaa tctataaaat
     1201 t
//
LOCUS       DAXWLG010000320         1174 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.329, whole genome shotgun sequence.
ACCESSION   DAXWLG010000320 DAXWLG010000000
VERSION     DAXWLG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.329"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..467
                     /locus_tag="ACGE4T_002838"
     CDS             <1..467
                     /locus_tag="ACGE4T_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFF8388034.1"
                     /translation="KTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFV
                     SVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPK
                     FMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            476..703
                     /locus_tag="ACGE4T_002839"
     CDS             476..703
                     /locus_tag="ACGE4T_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8388035.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          364 a          192 c          198 g          420 t
ORIGIN      
        1 ttaaaacatt gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg
       61 gcgtactttt ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga
      121 ccacattatt tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt
      181 tcttattgaa actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa
      241 acatactaaa aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac
      301 ttcctaaatt tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa
      361 tcgctgtcgt attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc
      421 ttgaacatgc gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc
      481 aattatcgtt aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag
      541 tgagaaagtg ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc
      601 catccgtttt tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt
      661 gttgacctat aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac
      721 agtaaataaa aaataatctg aatgatagat gtgtgagact tttagaagtt atttacgtct
      781 attattcaga ttttttattt cgattgaata ccgttttatc tataaataag atatgtactg
      841 actcatacaa agggggcctt ttcttctcca tgtactatca cttttcctga tacaactggt
      901 ttcctgttac gttgtagttg gatctgcttt tagtataata ctttattcat tccaccagac
      961 tgatagcagt ttgagcagtc taaataatca tgtagtatta caggccattc tgtttcatac
     1021 cttgaagtgc ttaaaacttc agctaatggc ccattgtgtt aactcttact ccattctttt
     1081 ttagtaagct cccatatata ctgtaaagcc ttctatatat tatgtcatgt tgctttttaa
     1141 catttactat gtagttttga taaaatgatt tgaa
//
LOCUS       DAXWLG010000321         1173 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.330, whole genome shotgun sequence.
ACCESSION   DAXWLG010000321 DAXWLG010000000
VERSION     DAXWLG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.330"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            48..308
                     /locus_tag="ACGE4T_002840"
     CDS             48..308
                     /locus_tag="ACGE4T_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388036.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(485..613)
                     /locus_tag="ACGE4T_002841"
     CDS             complement(485..613)
                     /locus_tag="ACGE4T_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388037.1"
                     /translation="MQPLQMLYDNKPELNDPDISFAFFEMIGSDDLFKTTSLSIRK"
     gene            complement(670..909)
                     /locus_tag="ACGE4T_002842"
     CDS             complement(670..909)
                     /locus_tag="ACGE4T_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388038.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          419 a          164 c          211 g          379 t
ORIGIN      
        1 gttattatca ttttaaataa tgaaaaaaca ttaaggatgg atgaaatatg aataacgaaa
       61 ctatcatttt aggaaaaaaa tatatttgcc aacctattgg cttgaaacaa aaagtgattg
      121 gagaagtaat cagtaaatta gaaaattgtg ttgtgatctg cgtagacgaa tatcatgacg
      181 tcgatcgcaa tgaaatatta gaaaaatgcg gaaaagtagt ggcaaaatat actcacgttt
      241 atgagttgga aacaacagaa tatctatata actatagcaa caaatatgaa catactttag
      301 tttcataaag atttaaataa agtgccttca aaagcgagaa gaccaagctt ttgaaggcac
      361 tttatttagt tggcagtgat tgttcatgat ttttgattta ttcaaaaggt tcgcctagag
      421 aatctgtctc tgtaacgagg cctggtggag aaactcgata aaaacctata ctgccagaac
      481 tgtcctattt tcggatagac aaactagtag ttttaaaaag atcatccgat ccaatcattt
      541 caaaaaatgc aaaactgata tcaggatcat ttagctcagg cttgttatcg taaagcattt
      601 gtaaaggttg catttagtta ggagtaaacg cgaaactatc atcattattt gctgaagaag
      661 atgaagaagc taattcagag tttgtttgaa ataaaggaga cgtgctgaca gtttctttac
      721 cctgtttttt gcagaggtag aaacttttat atcggaagtt tctaccggag aatttttctc
      781 atttgtagaa attgttgaat cgatactatt cgaaatttgc gtatctgaag gtatgttctt
      841 tgatgcagaa gtcccacaag cagtaagtaa caataaattt gataaactga taaacagaat
      901 tcttttcatt atgtacacac tccttatagt atcgtttagc atacacctat gctcagtatt
      961 aggcaaaatc aatgcaaaac tatcaagtct tttatagtat ctattagtct tagtaataca
     1021 tatatggaga gggtgatatc ccagttcaaa ccagatacat tggttaccat ctgtattaac
     1081 gattatgcct ctaaacgctt tttttatgaa aaaatttata agtgaaaacg taggtatgat
     1141 attatctaga ggtaattttt ttgggtattt aaa
//
LOCUS       DAXWLG010000322         1142 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.331, whole genome shotgun sequence.
ACCESSION   DAXWLG010000322 DAXWLG010000000
VERSION     DAXWLG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.331"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(257..493)
                     /locus_tag="ACGE4T_002843"
     CDS             complement(257..493)
                     /locus_tag="ACGE4T_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388039.1"
                     /translation="MDKGVADIAKIKQVLKQESIKSLVEGTGLSKSTISSLKSGTRKV
                     EKLNLFAAIKLTEYSDQVFKPIIEIWGKELKKQL"
BASE COUNT          412 a          173 c          124 g          433 t
ORIGIN      
        1 atcaaaaaaa attacttctt tatttatcat tgtatcaaca ggataaagaa actacagaat
       61 ttgaagaaga tcttctgaaa tatctgaaaa tattggtaaa aaaaagactc attgagattg
      121 aataaaatta taactttata attggtagaa ctgagaaata ttatctttca gttctaccca
      181 ttgtagagct aaacaagcat tcatctgaaa aatatctagt tcttctattt ttaaatctat
      241 tagttttatt cgataactat agttgttttt ttagttcttt tccccagatt tcaataatag
      301 gtttaaaaac ttgatctgaa tattcggtta gtttaatagc tgcaaaaagg tttaattttt
      361 ctacttttcg agtacctgat tttaaacttg aaatagtact cttacttaaa ccagttcctt
      421 ctactaagct ttttatagat tcttgtttca acacttgctt aatttttgca atatcagcga
      481 ctcctttatc caaaaaaaga ctcctttttt atctcaaatt tataactact gtagtaaaaa
      541 tatagcatat tttagtatgc tctggtagta aaagtttaaa agtacgaaaa gggaacaaat
      601 atacaataaa aataaattgt attacataag caacatagat atactccaac atcctattta
      661 tctgaataaa ggaacctttc aaaacaaaaa attattttga tggacgtcta atccagcata
      721 atactcataa ttatagagcc gatgttctta tatcactcta tcagtcctat ttgacaaaga
      781 gcctaaataa taccaaccgt cataaacttt tgttgtatta ttagtttttt catcttgggt
      841 atcaatattt gttggttcat aaagcttttc attttcatta tttgttttta aattttgagt
      901 aaatatcact ttatcattaa aattaaaccg aaaggttact acttctaact caggataaat
      961 aatcgatctt ttctttctaa aatttctcct aaataaatac tagttaaaat aactaatccc
     1021 ctatatcatc catcttttaa catcgttcta aaaaaattaa tatttagttg tgtgaactat
     1081 caatttatag ttatttatgt tatcactgaa atttaaccca caatagtagt ctctaagacc
     1141 at
//
LOCUS       DAXWLG010000323         1127 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.332, whole genome shotgun sequence.
ACCESSION   DAXWLG010000323 DAXWLG010000000
VERSION     DAXWLG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.332"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            121..936
                     /gene="thiD"
                     /locus_tag="ACGE4T_002844"
     CDS             121..936
                     /gene="thiD"
                     /locus_tag="ACGE4T_002844"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFF8388040.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          401 a          188 c          251 g          287 t
ORIGIN      
        1 aaatttgatt accaagaaaa ggtagtggta tatttactga gggatacttt gaaaaacata
       61 tgatagaaac agtaacagta ttatgaaaaa tctttttata aaaatggagg aatcgatttg
      121 atggaagatc agtatgtaca agcaatgacg atcgcaggat cagatagcga tggaagcgca
      181 gggatgcaag cagatatgca tacattcttt acaagaaatg tttacggtgt ttctgtcatg
      241 accgcatgtg ttgcaggtaa ttcttacgga atcggagcaa gtgtgacatt gccaacagat
      301 tttatcgaca aagaatttga actgattgcg aaggactttc aaatacgtgc agctaaaaca
      361 ggtatgctag cagattcaaa gttgattgag acagttgtaa aaaattacca aaaatatgat
      421 tttggaccat tagtcgtgga tcccgtaatc gtaacgaaac atggaaatct tttactagaa
      481 gaatctgctt tgcaaagctt gaaagaaaag cttgtccctt tggccgaagt actgacacct
      541 aatttttatg aagcagaaaa gctgactgga cggacattag cagctgaaga agattttgtg
      601 caagcagcaa aagaactgca agatatgggt gcaaaaaacg ttatgatcaa aggacatcat
      661 gctaaaaatg cgcagcaaga aacagtcaat gactacgtat tgttcgaatc aggaaaatca
      721 ttttggttaa atgcaccatt ttatcctacc gaccggatca acggaactgg cgattcgcta
      781 tctgcatgta ttgcagctga gttagcgaaa ggaaaaacag ttgaacaagc gatccgattg
      841 gcaaaagact atgtaaaccg tgcaattcgt gaagaaataa atgtagggca caaatatggc
      901 cccatcaatc attgggcagc tggtgaatta gagtaaaaga aagaagttgt gaaaaagttg
      961 ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggt
     1021 tggacttatt accgcagatg caagcttttg aacaccgttt aagaaagagg tcgtgagcct
     1081 gccgtccagc tccgagtcac aaggaacaag ttcaaaaaac agcttct
//
LOCUS       DAXWLG010000324         1124 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.333, whole genome shotgun sequence.
ACCESSION   DAXWLG010000324 DAXWLG010000000
VERSION     DAXWLG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.333"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..1008)
                     /locus_tag="ACGE4T_002845"
     CDS             complement(<1..1008)
                     /locus_tag="ACGE4T_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFF8388041.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NV"
BASE COUNT          321 a          247 c          176 g          380 t
ORIGIN      
        1 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
       61 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      121 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      181 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      241 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      301 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      361 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      421 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      481 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      541 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      601 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      661 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      721 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      781 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
      841 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
      901 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
      961 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1021 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1081 acaccttttc tttttctttt aaaataaatg ataaattcca ataa
//
LOCUS       DAXWLG010000325         1123 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.334, whole genome shotgun sequence.
ACCESSION   DAXWLG010000325 DAXWLG010000000
VERSION     DAXWLG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.334"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..886
                     /locus_tag="ACGE4T_002846"
     CDS             <1..886
                     /locus_tag="ACGE4T_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFF8388042.1"
                     /translation="YLYTAKYWIDDSRKTIKYAKRKKQFYFQTWHGTPLKKIEFDAKD
                     KLPKRYLSYAKKDSESITYLLSGNRYSSEKYVSAFNIVPSKILEVGTPRNDELASSKE
                     QVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVDNLREFFKKQQQELNLMTKFHPN
                     VHSKLATDSKSIEYMKKHQITLINDGIPLEMLFEEIDVLITDYSSIFFDFALTKKPII
                     LFNYDEKEYSLERGFYIQLSELPIISVQDSYGLVDVFKNQQEKLLKSSQELLDYIGNY
                     ETGASTEKVLELLERDIK"
     gene            883..>1123
                     /locus_tag="ACGE4T_002847"
     CDS             883..>1123
                     /locus_tag="ACGE4T_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8388043.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQ"
BASE COUNT          421 a          141 c          189 g          372 t
ORIGIN      
        1 ttatttgtat acagctaagt attggataga cgatagtcgg aaaacgatta agtatgcaaa
       61 aaggaaaaaa caattttatt ttcaaacatg gcacggtacg cctttaaaaa aaattgaatt
      121 tgatgcaaaa gataagttac ctaaacgtta cctaagctat gcgaaaaaag atagtgagtc
      181 aattacatat ttattatctg gtaatcgcta tagttctgag aagtatgttt cagcattcaa
      241 tatcgttcct tctaaaatat tagaagtagg aactcctaga aacgatgaat tagcctctag
      301 taaagagcaa gtgtttgact tgaaaaagaa acaaatcaat gtcttatttg ctccaacttt
      361 tcgaaataat attgaggaca acggaattac gcaactagaa tggttaggtg tggataactt
      421 acgtgaattt tttaaaaagc aacaacaaga attaaattta atgactaaat ttcatcctaa
      481 tgttcatagt aaattagcaa ctgattctaa aagtatagaa tatatgaaaa aacatcaaat
      541 tactttaatt aatgatggga ttcctcttga aatgcttttt gaagagatag atgttttaat
      601 cacagattat tcatctattt ttttcgattt tgcgctaaca aaaaaaccaa ttattctttt
      661 taactatgat gaaaaagaat atagcttaga aagaggtttt tatatacagt taagtgagtt
      721 accaattata agcgtacaag atagttatgg ccttgttgat gtttttaaaa accaacaaga
      781 aaagcttttg aaaagttctc aagagttact tgattatatt ggaaattatg aaacaggggc
      841 aagtacagaa aaggttcttg agttgttaga gagggatata aagtgaaaaa aacagtaaaa
      901 gatatatttt ataatgcgat atatcaagtc tttttgattg ttttgccctt acttactata
      961 cctatactta gtcgtagaat aggttcaact ggtttaggga tttatgggta tgttttctca
     1021 atatcacaat ttttaatgac tgtcattgct gtgggaatga atccttttcg tattcgaaat
     1081 atcgctaaat caaggaaaga taaaaaagca ctatctttac aat
//
LOCUS       DAXWLG010000326         1105 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.335, whole genome shotgun sequence.
ACCESSION   DAXWLG010000326 DAXWLG010000000
VERSION     DAXWLG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.335"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..1087
                     /locus_tag="ACGE4T_002848"
     CDS             <1..1087
                     /locus_tag="ACGE4T_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8388044.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DAXWLG010000327         1103 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.336, whole genome shotgun sequence.
ACCESSION   DAXWLG010000327 DAXWLG010000000
VERSION     DAXWLG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.336"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            262..345
                     /locus_tag="ACGE4T_002849"
     tRNA            262..345
                     /locus_tag="ACGE4T_002849"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Leu,seq:cag)
     gene            complement(669..>1103)
                     /locus_tag="ACGE4T_002850"
     CDS             complement(669..>1103)
                     /locus_tag="ACGE4T_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFF8388045.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          362 a          179 c          244 g          318 t
ORIGIN      
        1 agaagatagc tgaatcgaga aaaagaggaa aaaagaaatg aagaaaaaaa taaaatagtt
       61 actaatccca gagtcgttcg tttaacattg aaatcgatca tatggataaa acagattttt
      121 tcaccaaccc tcaatagatg atggagttgc tcggatgaat tatttgtgta gatggcataa
      181 gggagggcgt acagaaatga aaaagaacaa aaatatgttc ttgacaaaaa acacatgtaa
      241 tcctaaacta aaagatgtga tgcggctatg gcggaattgg cagacgcgct agcttcaggc
      301 gctagtgagg gtgacctcgt ggaagttcga gtcttcttag ccgcataatt tgttttttta
      361 taaagagaag gtacacctgg agcgatgaga aagaaaataa catattatct aaaagaatat
      421 aggaaaaatt ttaaacaacg gaaccagatt caaataaatc aaaaaaaatt tgtgtgaagg
      481 tgggacagtt taaaacctaa tagattgatt caaatagtag atttatagat tctgatacaa
      541 gaaatactgg caaacgatag ttaaggaata aagactaaac ttaaactcaa aaagaggtca
      601 tgcgaaagaa taaatcgcat gacctcttac tatattttga atgtgtttaa acgttcaaat
      661 aatgaatctt agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca
      721 taacgtgaag taacatattg ttctgctact ctttcttggt ttgcagcaga gtagtcacca
      781 tttaagtaag aagaatctaa ttggtaacgg ccgatgtaac gtccgttcgt tgctgtgtaa
      841 gaaccgcttg attctttttg tgcgatccat tcttttgcag agcttgtact tgctggagta
      901 gcagcagttt ctgttacagc aggagcaggt tgtgtttcag caacaggcgc tgcttgtgtt
      961 tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt gaacaggttc agcttggact
     1021 ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc ttctgttggg
     1081 attgttaatt gttgtcctga gta
//
LOCUS       DAXWLG010000328         1058 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.338, whole genome shotgun sequence.
ACCESSION   DAXWLG010000328 DAXWLG010000000
VERSION     DAXWLG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.338"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..915
                     /locus_tag="ACGE4T_002851"
     CDS             <1..915
                     /locus_tag="ACGE4T_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HFF8388046.1"
                     /translation="YAVKELTSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYF
                     VKGAYNLGDGKYLYSIYKDQDSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNN
                     LQWEKEIEKPQIGSVFSARLKLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLN
                     KYVTFSVNNEDFWSSLDVNYSKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWR
                     GYYKGNNFEDTQITMLENKSVKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNF
                     TNNAYYYELKKDGRVYGYINSEAAVEMN"
BASE COUNT          400 a          128 c          193 g          337 t
ORIGIN      
        1 tatgctgtta aagagttgac ctctgttcaa ataaacagat atttaacttc taaagatagt
       61 ggtcaaccaa cttggggaaa tctagagtgt actataagta aaggacaaac tacaaaagat
      121 aaaatatatt ttgttaaagg agcatataat ttaggggatg gaaagtatct atattctatt
      181 tataaagatc aggattcctc tgaatggtta gggtatttaa aagcacatgt tagtttagcc
      241 tatactccta ttgaagaaat taataaaaac gtaactgtta ctaaaaattg gtcgatttgg
      301 aataatctac aatgggaaaa ggaaattgaa aagcctcaga ttggttctgt attttcagct
      361 agattaaagt taaccaatgt atcgaataat gctgtctatt acaagcttta taaatctgga
      421 aaattttatg gttatatcaa tgctgaggct gtcaaagacc ttacaacaac aaagcttaat
      481 aagtatgtta catttagtgt taataatgaa gatttttggt cttctttaga tgtaaattat
      541 tcaaaaggaa aaactgaaag aggccgagta ttttacatta gtatttcata taataccgca
      601 gataaccaac cgatttattc agtatataca gatgaaacat gtacggaatg gcgagggtat
      661 tataagggaa acaactttga agacactcaa attactatgc ttgagaataa aagcgtgaaa
      721 gtaacaaaaa gtgggtatac agtatgggga gacttgaatt tttggactaa gtcaggaata
      781 tcgaacacag gagatatata caccactgac cgaaaatttt ataattttac caacaatgct
      841 tactattatg aattgaaaaa agatgggagg gtctatggct atataaatag tgaagcagct
      901 gtagagatga actaaaatat tgctgtccct taatcttatg gggcagcaat atttttatat
      961 tattaatcat ttttctgaag cgagggtaga agaattaatc aatagttttt cttgttttgt
     1021 aatataatag tttaattacc aaaaattatt taagtaaa
//
LOCUS       DAXWLG010000329         1044 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.339, whole genome shotgun sequence.
ACCESSION   DAXWLG010000329 DAXWLG010000000
VERSION     DAXWLG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.339"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            88..828
                     /locus_tag="ACGE4T_002852"
     CDS             88..828
                     /locus_tag="ACGE4T_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388047.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          387 a          182 c          201 g          274 t
ORIGIN      
        1 tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt attgagaaaa
       61 gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca tttccgtttt
      121 cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg agtattattt
      181 gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca gatgaaagaa
      241 tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc aacaaatgga
      301 ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt accggattat
      361 accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt tggtgattcc
      421 tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac acgaaacgca
      481 tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt acccgcatcg
      541 attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga atcgatgaaa
      601 aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca agaaaaaaga
      661 aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga aacaaagcag
      721 tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt acaaggattg
      781 atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt gatatacaaa
      841 tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt tatactctat
      901 ccgaaagttt atatcgtatc ctcaaatttt tttgagttta agcgaatttt ttctcccaat
      961 aagtaagaaa ataatggcaa aataaaaaaa caaactattt tcgcagatcg tttgcaaaaa
     1021 tagtttattt ttttataaag aaga
//
LOCUS       DAXWLG010000330         1003 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.340, whole genome shotgun sequence.
ACCESSION   DAXWLG010000330 DAXWLG010000000
VERSION     DAXWLG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.340"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>1003
                     /locus_tag="ACGE4T_002853"
     CDS             <1..>1003
                     /locus_tag="ACGE4T_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFF8388048.1"
                     /translation="QINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDNAYT
                     KFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDP
                     DFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLAR
                     KRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEK
                     TKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSS
                     MESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIG
                     "
BASE COUNT          359 a          183 c          201 g          260 t
ORIGIN      
        1 acagatcaat cgtatgatcg atcaaatgga gtctgtcaaa aaagaaagtt atttggaccc
       61 tactgttttt gaagcagagg aacatatcca aaaagtcatt cataaaatgt tccgtccaga
      121 caatgcctat acaaaatttg attttcgaaa tggccgtcca acgaagtctg cgatcttaac
      181 cacgagttcc attgatatgg ccaaaaagta ttatcgggcc atcaaagaga tgacgaaaga
      241 atctgattgg ttaataaaag agtttcccaa tcagcctatc cgtgaaggac gaacgatgga
      301 ggatcctgat ttcccacgaa tcgctattac ctattcatta gaagaaaata gccgagattc
      361 aagtgcccaa caagaagaaa tgcaaaaaat tattgaggaa tataatcagt attatgatac
      421 tgcctggtca ctagcagaca ttgagcgtta taacggagac atcaataatc gtttggcacg
      481 aaaaagagcg gaattcaaac aatttgggaa acaaatcgat ttagtaatcg tagtggatcg
      541 tctattgaca ggttttgacg cccctacgat tcaaacccta tttgtggatc gtaatctaga
      601 gtatgcagga ttgatccaag ccttttcacg tacgaaccgt acgtatcctg aaaaaacaaa
      661 aggactaatt gttactttcc gaaagccagc gacaatggag aagaatgtcg aggatgcgac
      721 aaaactgtat tcagaagcta aagaagaatc gggtttgatt tatccaagtt accaagaatc
      781 caaaaaaaga ttcaagcaag catatggtaa gttaaaagag gtttcatcca tggaatccat
      841 tgatgaacat acgccacttg aaatccgtat cgagtacgtc aaagcatttc aagaattgaa
      901 tcgtgcgtat gaatcgttag tgacttatga tgattacaat gatgacatgg tgcaatcttc
      961 aactttgaac aatcaaattc aacttttgga agaacaaatt ggc
//
LOCUS       DAXWLG010000331          999 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.341, whole genome shotgun sequence.
ACCESSION   DAXWLG010000331 DAXWLG010000000
VERSION     DAXWLG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.341"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>999
                     /locus_tag="ACGE4T_002854"
     rRNA            <1..>999
                     /locus_tag="ACGE4T_002854"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          310 a          198 c          288 g          203 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgag
//
LOCUS       DAXWLG010000332          996 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.342, whole genome shotgun sequence.
ACCESSION   DAXWLG010000332 DAXWLG010000000
VERSION     DAXWLG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.342"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            13..957
                     /locus_tag="ACGE4T_002855"
     CDS             13..957
                     /locus_tag="ACGE4T_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFF8388049.1"
                     /translation="MDTILITGGAGFTGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
BASE COUNT          333 a          150 c          209 g          304 t
ORIGIN      
        1 ggagtgaatt tgatggacac tatattgatt actggcggag ctggttttac cggttcaacg
       61 ctagcgaatt atttgggaaa agaaaacaaa atcgtagtag ttgacgattt atcaatggga
      121 aaaaaagaaa acttggatat ggataaacaa gtgactttca ttgaggggga tgtatcagat
      181 tctcaattga tggaaagaat catgaaagaa tatcagtttg cctatatttt tcatctagct
      241 gctgtagcta gtgtggcaga ttctgtagaa cggcctgtag aaacacatcg tgtaaatttt
      301 gacagtgcgc ttttattgct tgagttagca cggaaatatc aatctaattt aaaacgatta
      361 gtatttagtt catcagctgc agtttatggt gatgaaccaa cactgccaaa aaaagaagaa
      421 tcagtcatcc gtccattaac accatatgct atcgataaat ttgcagcaga acaatatgtt
      481 ttggattact gtcatttata tgatgttccg actagtgctg tccgattttt taacgtttat
      541 ggtccaaatc aaaatccaaa ctctccatat agcggagtca tttcgattct agtggatcgt
      601 tacaaaaaac aattggcagg agaaaaagcg gaatttactt tatttggcga tgggagtcag
      661 tctagagact ttgtttatat agaagatgtt atacaagcac ttttacttgt tgccaaggaa
      721 gaaaaagctt taggacagca attcaatgtc ggtacaggaa aaagcactac gctacttgaa
      781 ttgatccatt caattgatca aatcttagga acagagctag cgcttaaata tgaagcggag
      841 cgttcagggg atattcgtga ttccttagcg gatatttcaa aaattcgttc tttaggctat
      901 cagcctaaat ttgatatatt gagcggaatg gaacggtatt taaagacaga aatttgatga
      961 aaaaatgatt gcatctttgg ataaataatt ctaaaa
//
LOCUS       DAXWLG010000333          992 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.343, whole genome shotgun sequence.
ACCESSION   DAXWLG010000333 DAXWLG010000000
VERSION     DAXWLG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.343"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..901
                     /locus_tag="ACGE4T_002856"
     CDS             <1..901
                     /locus_tag="ACGE4T_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFF8388050.1"
                     /translation="EELPEGTKVNDVTPEGVIDTTQPGEYEGTIEVTYPDGTKDTVKV
                     PVEVVDNRNDAEKYTPEVEKEEVNYGGTIDLTDNVTNLEELPEGTKVNDVTPEGVIDT
                     TQPGEYEGTIEVTFPDGSSTTVKVPVTVKDKVDWVEINPIPTIDPEQIVPEEVHKGEE
                     ADLTDNILNLPEGSKVEVVTPVDTTQVGEHTGEVKVTFPDGSSVVVKIPIIVKNMDDM
                     ITNNQMTRVEKNSVVLDVINKSKSLSSSKIFGKSDNMYVETSNKELPKTGEENTTILS
                     ILGMLSISFCVLLFRLFKKREEN"
BASE COUNT          393 a          148 c          217 g          234 t
ORIGIN      
        1 ggaagagttg ccggaaggca cgaaagtgaa cgatgttaca ccagaaggtg tcattgatac
       61 aacccagcca ggtgagtatg aaggaacgat agaagttacg tatccagatg gaacaaaaga
      121 tacagtgaaa gttccagtgg aggtagtgga caatcgaaat gatgctgaaa aatatactcc
      181 agaagtagaa aaagaagaag tcaactatgg aggaacgatt gatctgacag ataatgtaac
      241 taatctggaa gagttgccgg aaggcacgaa agtgaacgat gttacaccag aaggtgtcat
      301 tgatacaacc cagccaggtg agtatgaagg aacgatagaa gttaccttcc cagatggatc
      361 aagtacaacg gttaaggtgc cagtaacagt caaagataaa gtagattggg tagaaatcaa
      421 tccaattcca acaatcgatc cagaacaaat cgtgccggaa gaagttcata aaggagaaga
      481 agctgaccta acagataaca ttctcaacct tccagaagga agtaaagtgg aagtcgtcac
      541 accagtggat actacacaag taggcgaaca cacaggcgaa gtgaaagtta ccttcccaga
      601 tggttcaagt gtggtagtaa aaatcccaat aattgtgaaa aatatggatg atatgataac
      661 taataatcaa atgacgagag tagaaaaaaa ttctgtagta ctcgacgtta taaataaaag
      721 taaaagctta tcttcatcga agatttttgg gaagtcagat aatatgtatg tagagacaag
      781 caataaagaa cttcctaaaa caggagaaga aaatactaca attttatcta ttttagggat
      841 gttatcaatt agtttctgtg ttcttttatt tagattattc aaaaaaagag aagaaaacta
      901 aagattaaaa atagcaaacg gagattattt ccgtttgctt attttctaaa aaaataaaaa
      961 acgaacactc atgttggcga tttagatgat at
//
LOCUS       DAXWLG010000334          978 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.344, whole genome shotgun sequence.
ACCESSION   DAXWLG010000334 DAXWLG010000000
VERSION     DAXWLG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.344"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            82..>978
                     /locus_tag="ACGE4T_002857"
     CDS             82..>978
                     /locus_tag="ACGE4T_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFF8388051.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYE"
BASE COUNT          333 a          182 c          192 g          271 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaa
//
LOCUS       DAXWLG010000335          966 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.345, whole genome shotgun sequence.
ACCESSION   DAXWLG010000335 DAXWLG010000000
VERSION     DAXWLG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.345"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(23..880)
                     /locus_tag="ACGE4T_002858"
     CDS             complement(23..880)
                     /locus_tag="ACGE4T_002858"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8388052.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
BASE COUNT          251 a          189 c          183 g          343 t
ORIGIN      
        1 aagactctgt tcttttttat ttctattccc ataatccagg taaagcttta ccgactattt
       61 tttcttgagt aaatggatgg atcatgtgca gttctaatgc atggagcatc agtcgttcag
      121 ctgttctatt ttggtagaga gggtctccaa cgatcggatg tcccttatga gctaaatgaa
      181 ctctgatctg gtgtgtacgt ccggtttcca aaacacaata gacttgtgtt tgttcagaaa
      241 gtactttgtt gacagtgaca tgtgtcaagg cgctctgacc atttttatca tctacgaccc
      301 tttttctccg atcatggcga tctcgtccaa ttttattggt gatggtcata tctttggcaa
      361 tttctcccca tacgatggct tgatatctcc gatagatcat tttttgttcc aacattcttc
      421 caagtatcgg caagacaaat ggattttttg caaataaaac ggcaccgctt gtttctttgt
      481 ccaagcggtg gacaacgtaa ggtcgttgct gtttagtgtc gagataagca gctaaatgat
      541 tgagtaaggt actgttttct gtcggttcat ttggatgagt cttcattcct gcaggcttat
      601 ccactataat caaatgctca tcttcatata aaacacggat gtgcttcgca tctcctaatt
      661 gaacttcttg ataattataa tctgtttctt cgaataacaa tgtgatctgg tcattgccct
      721 ttacttcatg atggaacaaa gcaggttgtt gattgatcca aacgttttta cgtatacgca
      781 aaaagtgtcg aacctttcta ggaactaacc attccttttc aagcaagtcg cgaattgttg
      841 tttcaggatg gttcataggt aaagtgatcg tgattttcac aagtgtactc ctttcagtgc
      901 aatcgcagac taattgtacc atttttaaaa catatttaag aaaaaactta catttttctg
      961 ttcatt
//
LOCUS       DAXWLG010000336          966 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.346, whole genome shotgun sequence.
ACCESSION   DAXWLG010000336 DAXWLG010000000
VERSION     DAXWLG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.346"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(56..286)
                     /locus_tag="ACGE4T_002859"
     CDS             complement(56..286)
                     /locus_tag="ACGE4T_002859"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFF8388053.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(380..718)
                     /locus_tag="ACGE4T_002860"
     CDS             complement(380..718)
                     /locus_tag="ACGE4T_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388054.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          308 a          196 c          106 g          356 t
ORIGIN      
        1 tatcgaaaaa acaataacat aatatgtgtc aaaatttttc ataataagaa ctcccttact
       61 gttgcatact aacataatcc aaaaactctt ctacttcagc taaagacatt ccactttctt
      121 tagtccaaaa aacagttttt tctatcgatc cttctctgta aactgtaaat gtcggagtcg
      181 agttaattga gtagcgactc ttgaattctt tccactcatt ttcatcttct ctaacttttg
      241 ctacattcac gtaataaata ttttcttttc ccttttctac cagcatatcg aatagcttcc
      301 tgcttttgat gatcgatttc ttgtgctaca tctagtaaat tttcatcaat ttctatatca
      361 taaagtattt cactagtgtc tacatccaca atatatactt tttatcagta gcaatttttg
      421 agtaatccgt taaaatgatc tcagtctcgt cactttcatc agtataaata tacgttaaca
      481 atgaactcac cttttcaatc ggtgttagct ctaagcctac tgtatttttt actattgagt
      541 cattatttac caccataact tcgtactgta tataccatcc tatttggttt ccatcaatat
      601 cagtaattgg ctcactagct aatggagtct ccggaacaat attttcctga tatttcttaa
      661 actcttctgt agcacgaaaa aggttatcat gttcctcact tgaaactggt tgagtcatag
      721 aaaatgttac ttggctaaac agagggaaaa taaacactac aatcaacaaa gtataaaaaa
      781 aagcccttct ttttcatttt ttatcttcca atcacaaatt gaaaaactta ttcctgcaac
      841 tttgagtcta gtatcacaat cgactgacta caactattca tttacattct actttaagtt
      901 cctccttaac cattaatttt attaaagata cttgtaatag tgattctcac aaaatttcat
      961 gaaaaa
//
LOCUS       DAXWLG010000337          961 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.347, whole genome shotgun sequence.
ACCESSION   DAXWLG010000337 DAXWLG010000000
VERSION     DAXWLG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.347"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..382
                     /gene="spx"
                     /locus_tag="ACGE4T_002861"
     CDS             <1..382
                     /gene="spx"
                     /locus_tag="ACGE4T_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFF8388055.1"
                     /translation="TSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEGGVQ
                     DIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDEIRS
                     FVPREIRCVARNLIYLQNNNILNS"
     gene            396..752
                     /locus_tag="ACGE4T_002862"
     CDS             396..752
                     /locus_tag="ACGE4T_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8388056.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          325 a          152 c          186 g          298 t
ORIGIN      
        1 tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat caacttgaat ttgaagagaa
       61 gaatattttt gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga
      121 aggaggagtt caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga
      181 ttttaacgaa ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt
      241 gagacgacct attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat
      301 ccggtcattt gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa
      361 caataacatc ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag
      421 cgcactgtgt aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc
      481 aatttgttga cttagaagaa cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt
      541 ttgaagaagt gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg
      601 cgattgacct ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca
      661 atctcctcag acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc
      721 agttgatgga acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga
      781 gcatttcata cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt
      841 ttgactttat ttttctacta tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta
      901 ggtggggata tagagttaaa aatatattga catgtgtaaa tttacatgat atattcattt
      961 c
//
LOCUS       DAXWLG010000338          951 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.348, whole genome shotgun sequence.
ACCESSION   DAXWLG010000338 DAXWLG010000000
VERSION     DAXWLG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.348"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..96)
                     /locus_tag="ACGE4T_002863"
     CDS             complement(<1..96)
                     /locus_tag="ACGE4T_002863"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8388057.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTI"
     gene            complement(180..650)
                     /locus_tag="ACGE4T_002864"
     CDS             complement(180..650)
                     /locus_tag="ACGE4T_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFF8388058.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            complement(667..>951)
                     /locus_tag="ACGE4T_002865"
     CDS             complement(667..>951)
                     /locus_tag="ACGE4T_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8388059.1"
                     /translation="GYRFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWR
                     AKDRILNLPLGTHEADFKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
BASE COUNT          237 a          152 c          145 g          417 t
ORIGIN      
        1 aatggttttt ttagatacta aaagcatttc agctaaatct tcttgagtta gttgtttttc
       61 tattcttttt tgtttgatta ctttacttat agtcatttga tcacctaaac atatttttta
      121 ttcttagaaa gatagagtct atagaaattg atttcttctt tatggaaagt tggtaattgt
      181 taccaaaatt tccaccactt tttttctgtt gttattgcat tgttgttttc tttattgaca
      241 gaagttaatt cctcttcttc aggttcttcc gtataagcta attgtaactg ttcttgcagt
      301 ttttcgatct gttgatttgc ctgcaaggtg agttgttgtt gctggtcgag taacttttgc
      361 atttgtttta tttgagtatc tttttcttgg agctgttcat gtaaaaaaac aataatcgag
      421 ttttcatcgt tactagcaac ggtttctact tggttaccag tggtgttact acctaaaaaa
      481 tgttgtttta aaagcaaata tcctttgtag ctgacaacta aggttttcac tccgttccta
      541 gtaacagttt gcacatagtt ttctctaaaa tcagctgata gccttttcct aattgcttgt
      601 tttgaaacat gaaattcttc cgcgagttct ttgatcgttt tatctatcat aaatactcct
      661 tatgcttcaa tcgaataagt tttgtaagtt ttctaataaa tcttgtttaa gctcatttga
      721 gattgattgt ttttccgctt cttctttttc ggtttgttgt tgttttttaa aatcagcttc
      781 atgagtacct aacggaagat ttagtattcg atctttagca cgccattttt ctttttgtgt
      841 taattctcca ttgtgttcaa tattaaataa ttttatacgt aaatcttctt gcttcccttt
      901 tgaaaaatcg tcagcatggt tgatttcagc tttccaagta aaacgataac c
//
LOCUS       DAXWLG010000339          944 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.349, whole genome shotgun sequence.
ACCESSION   DAXWLG010000339 DAXWLG010000000
VERSION     DAXWLG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.349"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACGE4T_002866"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACGE4T_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8388060.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          279 a          195 c          140 g          330 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acct
//
LOCUS       DAXWLG010000340          913 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.350, whole genome shotgun sequence.
ACCESSION   DAXWLG010000340 DAXWLG010000000
VERSION     DAXWLG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.350"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(128..>913)
                     /locus_tag="ACGE4T_002867"
     CDS             complement(128..>913)
                     /locus_tag="ACGE4T_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFF8388061.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGK
                     KWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQ
                     TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYV
                     HWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          245 a          213 c          175 g          280 t
ORIGIN      
        1 gaatggcaac actttttttg acaaaaatta taaggtgttg tgaatgctgc ggtgttatgg
       61 ctacgattgc ccttgacaat gagcatcctc ggatctgatt ttctccggca gaagcacaat
      121 cattcaatta cgctgcctct ccagaggtat cagatccgcg ctcattatca aggattcgct
      181 tcgccaatct ctgttgtcga atttggatcg gtgtttcata cgccaacgtg ccatgtaagc
      241 gtagatagtt ccaccaatgc acataatcaa acaattctag acgtagctgt gccagtgtct
      301 caaattggta ttgatgcacg aattctactt tgacagattt ataggttgat tccacaacgg
      361 cattatcata cggacaaccc ttcctactca atgaacgagt aattccaaaa ccattcagaa
      421 tctcatgaat ggtttggtta tcaaattcct ttccccggtc tgtatgaaaa agcttgacgt
      481 ctgttaaaga atacggtatc cgtccaaagg cttcttttac caatgaggca tctttcttct
      541 caccacagga ataaccaata atttctcgat taaacaaatc aagtattaag cagatataat
      601 gccacttttt ccccacgcga acataagtaa gatccgtaac gatggcttcc aatggctgtt
      661 cttgtgtaaa ggtccgatct aatacattcg ctgttttcgc ttcattacaa gctgttcgtt
      721 gccctttaaa atgagcgatc gtataggtag atgtcaatcc gcggcgtttc atgattcgac
      781 cgattcggcg ccgactgagt tgaagcccac gctttgctaa acacttcttc aattttcggg
      841 taccgtaagc ctttcggttt cgaataaact cttcctgaac gatctcttct aagtcggact
      901 cattttcgat cgg
//
LOCUS       DAXWLG010000341          909 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.351, whole genome shotgun sequence.
ACCESSION   DAXWLG010000341 DAXWLG010000000
VERSION     DAXWLG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.351"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            90..839
                     /gene="pnuC"
                     /locus_tag="ACGE4T_002868"
     CDS             90..839
                     /gene="pnuC"
                     /locus_tag="ACGE4T_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFF8388062.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT          260 a          171 c          175 g          303 t
ORIGIN      
        1 gactagtttt ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact
       61 acatttagtg tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc
      121 agctaaaagg ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg
      181 tcatgactct tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca
      241 cgcttggtgt attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag
      301 gtattttgtc tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta
      361 tcggtgaaca gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat
      421 ggaatcataa tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa
      481 tcatcagtac tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg
      541 atcctcggcc gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca
      601 gtttcttgcg ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg
      661 tcctttggta tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct
      721 ctgtttactt gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag
      781 aacgtgaacg tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa
      841 aaaactgagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
      901 acggcggaa
//
LOCUS       DAXWLG010000342          903 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.352, whole genome shotgun sequence.
ACCESSION   DAXWLG010000342 DAXWLG010000000
VERSION     DAXWLG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.352"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..>903)
                     /locus_tag="ACGE4T_002869"
     rRNA            complement(<1..>903)
                     /locus_tag="ACGE4T_002869"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          193 a          277 c          203 g          230 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 ggg
//
LOCUS       DAXWLG010000343          876 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.353, whole genome shotgun sequence.
ACCESSION   DAXWLG010000343 DAXWLG010000000
VERSION     DAXWLG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.353"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>876
                     /gene="bsh"
                     /locus_tag="ACGE4T_002870"
     CDS             <1..>876
                     /gene="bsh"
                     /locus_tag="ACGE4T_002870"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFF8388063.1"
                     /translation="DHYFGRNFDYEISYNEVVTITPRNYKLNFRKVNDLDTHYAMIGI
                     AAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTV
                     GEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTN
                     NPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKAT
                     FTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYY
                     RTYEDSQITAIDMNK"
BASE COUNT          302 a          118 c          159 g          297 t
ORIGIN      
        1 tgatcattat tttggaagga attttgatta tgaaatatct tacaatgaag tagtcactat
       61 tactccaaga aattataagt tgaattttcg aaaggtaaat gatttggata ctcattatgc
      121 aatgattggt attgccgctg gtatagctga ctaccctctt tattacgatg cgacaaatga
      181 aaaaggattg agtatggctg gactaaattt ttctgggtat gctgattata aagaaataca
      241 agaagggaaa gacaatgtat ctccttttga atttattcct tggattttag gacaatgctc
      301 aacagtagga gaagctaaaa aattgttaaa aaatatcaat ttagcaaata taaattatag
      361 tgacgaactt cctttatccc ctttacattg gctattagct gataaagaaa aatcaattgt
      421 cattgaaagt atgaaagatg gacttcatat atatgataac cctgtgggcg ttcttaccaa
      481 taatccttca tttgactatc aattatttaa tttaaacaat tatcgtgtct tatcgagtga
      541 aactcctaaa aataattttt caaatcaaat aagtttgaat gcctatagcc gcggtatggg
      601 agggataggc ttgcctggag atttatcctc agtatctcgt tttgttaaag cgacttttac
      661 gaagctgaat tctgtatctg gagattcaga gtcagaaagt attagtcaat ttttccatat
      721 cttaggttca gtagaacaac aaaaaggttt gtgtgatgtt ggtgatggaa aatatgaata
      781 tacaatttat tcttcttgtt gcaatgttga caaaggaatc tattattatc gaacatatga
      841 agacagtcaa attactgcaa ttgatatgaa taaaga
//
LOCUS       DAXWLG010000344          872 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.354, whole genome shotgun sequence.
ACCESSION   DAXWLG010000344 DAXWLG010000000
VERSION     DAXWLG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.354"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(30..734)
                     /locus_tag="ACGE4T_002871"
     CDS             complement(30..734)
                     /locus_tag="ACGE4T_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388064.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          243 a          166 c          151 g          312 t
ORIGIN      
        1 atcccagcct tttatgacaa atagaatttt tatcgatcag taatcgctgt cgctgcaaat
       61 tgttcgatca aaggaatcaa agtcaatttg actttgtatt tccgttcgag tgctttttcg
      121 ataacgaact cattttcagt agtcatagaa atagttgcta atttacgagg ttcatcagca
      181 tttttttcat taataactac ttcatgttca ttttgttctg tgtaaaattt aggatactca
      241 gcaacggctt ttgaaaaatc agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta
      301 ttttcagttg tgtccgcttt tggtttttcc gttttttctt ctggcacact gactgtttct
      361 tctgtaactg gtgtcgcaac agcttgcgca gttggttcat cgtttttcaa tcgttgatca
      421 ttccagtaag acacgatttc aataagaaga ttgtcacgga atcgttcgaa aatcccgttt
      481 tctcctgaac gaattctctt taatacagta ttgaagttgt catcgattgg cacgttgatc
      541 cgtgtatctt catttgtttt cccagctaat ccgatcgtta agatttgacg gtttttggga
      601 tcaagcatga cttgtaacgg caaaccataa cgtcgcaagc tctcttcttc tagagaacgg
      661 aataattgtc ctcttacacc atccaatgaa ggacggaatt gatttaataa tttttgtttc
      721 tgttttttct tcatatgttc tctccttcgc tcataccctt tcgattataa gaaaaaaaca
      781 gccaaatgaa aaggagtaat gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat
      841 ttgcgtattt tcccgttttt tgaaagaaaa at
//
LOCUS       DAXWLG010000345          863 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.355, whole genome shotgun sequence.
ACCESSION   DAXWLG010000345 DAXWLG010000000
VERSION     DAXWLG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.355"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..602)
                     /locus_tag="ACGE4T_002872"
     CDS             complement(<1..602)
                     /locus_tag="ACGE4T_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8388065.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          274 a          153 c          176 g          260 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaattt gatcaaagtg gtagctgtta ttgaattcaa gaattgtttc tcgtatgaat
      481 gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa ttgattgtcg
      541 tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac aaataatttc
      601 atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac tgtgggattt
      661 cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat aaaagagcta
      721 gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca gctaatgagt
      781 tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac gctacaggag
      841 gaaatagcaa attcccgttg gat
//
LOCUS       DAXWLG010000346          855 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.356, whole genome shotgun sequence.
ACCESSION   DAXWLG010000346 DAXWLG010000000
VERSION     DAXWLG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.356"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..624
                     /locus_tag="ACGE4T_002873"
     CDS             <1..624
                     /locus_tag="ACGE4T_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388066.1"
                     /translation="NSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITS
                     NKTSRVLLALMGYGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLED
                     LFRLAEDDKYRFEVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAG
                     DQVKVLNRNSTDVVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            718..>855
                     /locus_tag="ACGE4T_002874"
     CDS             718..>855
                     /locus_tag="ACGE4T_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8388067.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          338 a          114 c          147 g          256 t
ORIGIN      
        1 aatagctatc tcaagattcg attttcacat atattaattt tactaccata tttgaatgag
       61 aatttcaccc taattcaagt agataagtct ttcattgcct caggtaatca aggaaatatg
      121 aatattacaa gtaacaaaac gtcacgtgtg ctattggcgt tgatgggtta cggaattctt
      181 tatagagatg accaaaatgg ccagattcta ataaatcggg ctgggcgttc attattaatg
      241 aaatatagaa aatttatacc ggaagatttt caaaacttac agttagaaaa catgcgttta
      301 gaagatttat ttagattagc agaagatgat aagtatagat ttgaagtttt gaaaattcaa
      361 aagaatgaac tattgaataa gttatatcct gagaatgcga taaaagcaca ttttggtgat
      421 gctaatcata tgtatagttg gttcattcct aaaagattta gagggaaaat caatgctgga
      481 gatcaagtga aagtactcaa tcgaaatagt acagatgtcg taatgataac agatatctat
      541 gtagaagatg aagatataat caaatatatg aagcctgtga taaaacttgt taataaaaat
      601 aaaggaaccc gtggtgctag ttaatttttt cctagagaaa aatgtctaaa catgttggta
      661 aatcaacaaa aaaacgcata tagtagtctc aaaactccca agaaatgagg actacatatg
      721 cgttcatcaa aatatactga acattataca gatactttta taacattcaa ggaaattatg
      781 agaactgttt ctaacatata ccacaattgt gtaccagata agattaaaaa ccgaagaaat
      841 actgatcaac tgaag
//
LOCUS       DAXWLG010000347          832 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.357, whole genome shotgun sequence.
ACCESSION   DAXWLG010000347 DAXWLG010000000
VERSION     DAXWLG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.357"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..693
                     /locus_tag="ACGE4T_002875"
     CDS             <1..693
                     /locus_tag="ACGE4T_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8388068.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          304 a          132 c          154 g          242 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccacttcaa gcgttaagaa ccgtcaggtt ta
//
LOCUS       DAXWLG010000348          831 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.358, whole genome shotgun sequence.
ACCESSION   DAXWLG010000348 DAXWLG010000000
VERSION     DAXWLG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.358"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          280 a          112 c          159 g          280 t
ORIGIN      
        1 agatatttta aaggatgttg aagaaaaagt ttataccaga gatatctttg gtgataacta
       61 aataataatg aacgggtaca tggaggaaaa tattttagat ggtgactcga aataaatgtt
      121 tgttctcatt gcatctgtat ctataccgat aagtttaaaa attccgatta ggaaaatgaa
      181 gttggctatc cttgcgagga atttattgct ggattatttt atgaactaaa gcaagacatc
      241 atcagaaaaa aatccaaaat ttgacaatgg gattgatcgt ttaccagctg taagcgaagt
      301 atctgacacg aaaatggaaa atgcaattac tgttattacc aatagctttt tttcatcgat
      361 caagtatttg aaaacattat ttgtttgcca agctgatcaa tgttctttca gacttggaag
      421 aacattgatc agctcacaag taatgattga agtttaggtt atgggataaa ggataagtga
      481 aataaaagtc gtgtctgcat ctttgttagt accagtaaat ttttctacta cggcgctagt
      541 cacgacagtc caggcatctg aaattgaaga tgcaaataga gtttctgcta gtttaggtat
      601 tgatgttact gtattagcat taatcttaga gccagtagca tccaataggt gtagctatag
      661 ttctaatagt ataaaacaat gcagaggata tgattgcttt atgtctcggt gtgtcaggtc
      721 aaaatagagt tccatatggg gcgatttctc aatgaataat cttatagata taatcttgtt
      781 tgctatttcg ttatcacttt tacttttctt cgattatttc aaaaataaaa a
//
LOCUS       DAXWLG010000349          830 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.359, whole genome shotgun sequence.
ACCESSION   DAXWLG010000349 DAXWLG010000000
VERSION     DAXWLG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.359"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..830
                     /locus_tag="ACGE4T_002876"
     rRNA            <1..830
                     /locus_tag="ACGE4T_002876"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          204 a          178 c          262 g          186 t
ORIGIN      
        1 catggagctt tactgtagtt tgatattgag tgtctgtacc gcatgtacag gataggtagg
       61 agccgtagaa atcggaacgc tagtttcgat ggaggcgctg gtgggatact acccctgcgt
      121 tatggccact ctaacccgca ccactaatcg tggtgggaga cagtgtcaga tgggcagttt
      181 gactggggcg gtcgcctcct aaaaggtaac ggaggcgccc aaaggttccc tcagaatggt
      241 tggaaatcat tcgaagagtg taaaggcaga agggagcttg actgcgagac caacaagtcg
      301 agcagggacg aaagtcgggc ttagtgatcc ggtggttccg catggaaggg ccatcgctca
      361 acggataaaa gctaccctgg ggataacagg cttatctccc ccaagagtcc acatcgacgg
      421 ggaggtttgg cacctcgatg tcggctcgtc gcatcctggg gctgtagtcg gtcccaaggg
      481 ttgggctgtt cgcccattaa agcggcacgc gagctgggtt cagaacgtcg tgagacagtt
      541 cggtccctat ccgtcgcggg cgttggaaat ttgagaggag ctgtccttag tacgagagga
      601 ccgggatgga cttaccgctg gtgtaccagt tgttctgcca agggcattgc tgggtagcta
      661 tgtagggaag ggataaacgc tgaaagcatc taagtgtgaa gcccacctca agatgagatt
      721 tcccatttct ttaagaaagt aagatccctg agagatgatc aggtagatag gtcaggagtg
      781 gaagtacagt gatgtatgga gcggactgat actaatcgat cgaggactta
//
LOCUS       DAXWLG010000350          819 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.360, whole genome shotgun sequence.
ACCESSION   DAXWLG010000350 DAXWLG010000000
VERSION     DAXWLG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.360"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            21..290
                     /locus_tag="ACGE4T_002877"
     CDS             21..290
                     /locus_tag="ACGE4T_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8388069.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            283..540
                     /locus_tag="ACGE4T_002878"
     CDS             283..540
                     /locus_tag="ACGE4T_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8388070.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          310 a           93 c          160 g          256 t
ORIGIN      
        1 ataaataagg ggtgaaagga atggaagcag tagcttattc aaatttccgc caaaatttac
       61 gtagttatat gaaacaagtt aatgaggatg ctgaaacact tattgtaaca agtaaagatg
      121 tagaagatac agttgttgta ttatcaaaaa gagattatga ttctatgcaa gaaacgttga
      181 gaacactttc taataattac gtcatggaaa aaattcgtcg aggagatgaa caattctcca
      241 aaggtgcatt taaaacacat gacttaatcg aggttgaatc tgatgattaa ggcttggtct
      301 gatgatgctt gggatgatta tctttattgg catgagcaag gaaacaaaag caatataaaa
      361 aagattaaca agttaataaa agatatcgat cgttccccct ttgctggatt aggaaaacct
      421 gagccattaa agcatgattt atctggaaaa tggtccagaa gaattacaga tgaacataga
      481 ctgatatata gagttgaaaa tgaaacgata tttatttatt ctgcaaaaga tcactattaa
      541 ccaatcggaa gtaaggaaag ggtcagaaac ttaaaagttt ttgatcctta ttttatttac
      601 cctagtcatt taaaaagcta atatagctta gtgttgattg ttattaatga atgtgtttgt
      661 tacgcgtatt acggatataa ggttagtaaa atcatttcta aagttgagga aaagtaaata
      721 taaatggctt aaatttcaac aatttgaagt tgaatagata tgttataata ctattgtagt
      781 gtgggatgtt agttactaaa ggatgacgct tatatatat
//
LOCUS       DAXWLG010000351          816 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.361, whole genome shotgun sequence.
ACCESSION   DAXWLG010000351 DAXWLG010000000
VERSION     DAXWLG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.361"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            15..548
                     /locus_tag="ACGE4T_002879"
     CDS             15..548
                     /locus_tag="ACGE4T_002879"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFF8388071.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          259 a          157 c          159 g          241 t
ORIGIN      
        1 gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat catcagcctt
       61 atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt gatgaattct
      121 atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt cacggaatcc
      181 atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact atccaaccat
      241 attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca aaggtcttag
      301 cgggatgctt agattattat catcttgaac agcctaatta tctttcttta tgtacagtga
      361 aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact gtgtgcgaaa
      421 atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt gcatgtgcgg
      481 aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa cttgtgacta
      541 taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg tttcactccg
      601 agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt gtgactggtg
      661 accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag ctgttctgca
      721 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttggactt
      781 attaccgcag atgcaagctt ttggacaccg tttacc
//
LOCUS       DAXWLG010000352          807 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.362, whole genome shotgun sequence.
ACCESSION   DAXWLG010000352 DAXWLG010000000
VERSION     DAXWLG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.362"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            198..566
                     /locus_tag="ACGE4T_002880"
     CDS             198..566
                     /locus_tag="ACGE4T_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFF8388072.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          279 a          131 c          118 g          279 t
ORIGIN      
        1 ctcaaaatat atattttcgt gtaagaattt ctttgccaag tcaatcattt ttatgtctct
       61 tttctatttt attatttgag acatcttatc atataaatat agcggttaca acattacttg
      121 tgttttgttc gttttttata cataactatt gtttttctgg taaaataaat caaaccactt
      181 aatggaggcg ttttagaatg gaaaaatttc agccgatcat caatcagatc aaagaaaaag
      241 aacatcaaca aaagatcaaa gacctttttg ctaagataac agaaacgtat ccccagctag
      301 acacaactgt aaaatggaac caacctatgt ttactgacca tggtaccttt attattgcat
      361 ttagttttgc taaaaatcat ttttctatcg caccggaaaa agcagcgatt cgtgcccttg
      421 aaaaaaatat ccaagaagca ggctacgtat atacggataa cgtcatcaaa gttccttgga
      481 aaagtgtaat caactgggaa ttgattgaac aattgatttc atttaatatt gaagataaaa
      541 aaggtcatga taaattttgg cgataaagct ttatacgaat aaacctttta ctggcgagga
      601 aactattcat cagcttatac aggttttgtt caatcctatc ctttttatca tccctggtta
      661 aacacttaat tgacaaagta tctgctattt ttataaggag tctttcttgt gctgaaaaaa
      721 atcactgatt gaatggcttt gaattttcca cttagtaaaa ttattttttc agcaattttc
      781 ctgcaaaact tttgaataag gaaaaaa
//
LOCUS       DAXWLG010000353          802 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.363, whole genome shotgun sequence.
ACCESSION   DAXWLG010000353 DAXWLG010000000
VERSION     DAXWLG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.363"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..613)
                     /locus_tag="ACGE4T_002881"
     CDS             complement(<1..613)
                     /locus_tag="ACGE4T_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFF8388073.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          240 a          156 c          147 g          259 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat tt
//
LOCUS       DAXWLG010000354          784 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.364, whole genome shotgun sequence.
ACCESSION   DAXWLG010000354 DAXWLG010000000
VERSION     DAXWLG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.364"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>784
                     /locus_tag="ACGE4T_002882"
     CDS             <1..>784
                     /locus_tag="ACGE4T_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFF8388074.1"
                     /translation="VKVLLNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLE
                     FKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEI
                     SKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNI
                     GSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRK
                     QEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQ"
BASE COUNT          346 a           82 c          134 g          222 t
ORIGIN      
        1 aagtgaaagt attactcaat aaactaacta tttctaatat agactcgact agggaaaaaa
       61 tcataaaaat atttgataaa attaataatt tattcaagaa tgacaatgct gaattaagga
      121 agggtctttt agagtttaaa cttcaagaac tagaacttca ttactcttat ttagtgaaaa
      181 ttaccgaaga aaaagaacaa caaaaagcaa taaaagaaca gatgatagaa gaggaaaaag
      241 ttcgtagaga aattgatcgt gaaaagaaac gtattgataa agaacaacgt caattcaatt
      301 ctgaaatttc caaattaatc gcatatatgc aaaaagctaa tgcagatgtg gaaaaagaat
      361 tatatgcaaa taaaattcaa gaactggaag aaaaattaaa agaattagaa gttatacgtg
      421 aaaatgtatt acaaagagaa ttaaatacta gagccggtta tgtgtatgta attagtaata
      481 ttgggtcttt tggtgaagac atatataaaa ttggtatgac tagaagatta gaacctatgg
      541 atagaataaa agaattgagt agtgcatcgg ttccatttga atttgacgtt catgcgatga
      601 ttttttcaga ggacgctccg tctttggaaa ctaaattgca taatcatttt agaaaacaag
      661 aagttaataa aattaatcaa agaaaagaat ttttcaaagt ttcactagac gaaatagaga
      721 aagttgtttt agaaaattat aatggaacag taacatttac aaaattagct aaagctgaac
      781 aata
//
LOCUS       DAXWLG010000355          756 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.365, whole genome shotgun sequence.
ACCESSION   DAXWLG010000355 DAXWLG010000000
VERSION     DAXWLG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.365"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          249 a          130 c           99 g          278 t
ORIGIN      
        1 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
       61 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      121 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      181 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      241 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      301 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      361 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      421 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      481 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      541 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      601 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      661 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      721 cattttttat aaacgatata tttttattgt ttttcg
//
LOCUS       DAXWLG010000356          754 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.366, whole genome shotgun sequence.
ACCESSION   DAXWLG010000356 DAXWLG010000000
VERSION     DAXWLG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.366"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..712)
                     /locus_tag="ACGE4T_002883"
     CDS             complement(<1..712)
                     /locus_tag="ACGE4T_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFF8388075.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIG"
BASE COUNT          220 a          137 c          100 g          297 t
ORIGIN      
        1 ctccaataat agctttctta atttcatgac taaattgtaa atcattccat attgaccaag
       61 cttttgtaac agttaccatt ttattatagt caatataagg gtagtcaggt tcatcaggca
      121 aaattctttt tgcaaagtta ggataaaaat attgtatgtt aacctctttt acagtttctc
      181 caggttgcgg tgcatgaatc attacaccgt tacctttata tatacctaca tgataagttg
      241 cacctcgatt gccgtaaaat aataaatcac caggcttaag tgaatttaaa cttacctctg
      301 taccgtattg ctcttgattg gtggttccca taggcagttc aatatttaca gcttgtttgt
      361 aaacatattt cactaaacca ccacagtcaa aagaatttgg tcccgatgct ccccattcgt
      421 atggctttcc aatttgctcc attgctttat taatgacttt ggtttgatta gaagtcatag
      481 tataatttac tattcttgtt tcactatttt catttgataa atttgtaggt atttccataa
      541 tctgcttaaa ctgttcttca ctataaccaa cttttaaagc tttttctttt tctatattat
      601 actgttcttc tgtgtagttt atttgttctg aaaaaacaat agtattgtag tacacactca
      661 aaacgattga gaaagacatc acaagaaata aagttaatag ttttttcttc attttttacc
      721 tcttctcctt attttttaca agcgcttgca tttt
//
LOCUS       DAXWLG010000357          752 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.367, whole genome shotgun sequence.
ACCESSION   DAXWLG010000357 DAXWLG010000000
VERSION     DAXWLG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.367"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(95..493)
                     /locus_tag="ACGE4T_002884"
     CDS             complement(95..493)
                     /locus_tag="ACGE4T_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFF8388076.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(490..726)
                     /locus_tag="ACGE4T_002885"
     CDS             complement(490..726)
                     /locus_tag="ACGE4T_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8388077.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          234 a          133 c          117 g          268 t
ORIGIN      
        1 atgaaaaaac tattttgtat gattaaacat aaaaaaatca gcatattcct taatgacatg
       61 ctgatctaat tgagaaataa agtttttaat tcgttcattt tattgcggtt tgtcgtaaat
      121 aactggttat cttggacttt agtgaatcga aatccaatga actagtggta atcattatga
      181 taaaatcaac agcttcttga cgagaaaaag caactggaca attattcaat tgaagaaaca
      241 acaacatggc aacaagagca gttcgtttat tgccattatg aaaaggatgt tttttagcta
      301 aattaattgc taaaatcgcc gctttttcat agatttcagg atagagttct tgattgaata
      361 ctgcttgtga aggctgtttg acggccatat ctaatgcatt tgcatctttt acaccgatca
      421 tttcgcctgg cgagtgacgt aaaataactt tcgcgttgat tttgataatt tctttagtcg
      481 aaagatacct cataaatcaa ccaaatcttt taacgcgtct tcatattgat tcatgatcga
      541 atcaactaat gaatcaatat caggttcttc ttgtgctttg atcattcgta cagaaccatc
      601 agattgaaaa acataactga ttgcgtcacc tgtctgcaca cccatgaatt cttttacttc
      661 tggtgggata gttgttacat cgctattgcc agattttcta attttatact ttttcatttt
      721 tttcatattt cgccaccctc tataccattc tc
//
LOCUS       DAXWLG010000358          731 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.368, whole genome shotgun sequence.
ACCESSION   DAXWLG010000358 DAXWLG010000000
VERSION     DAXWLG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.368"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(210..389)
                     /locus_tag="ACGE4T_002886"
     CDS             complement(210..389)
                     /locus_tag="ACGE4T_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388078.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(405..605)
                     /locus_tag="ACGE4T_002887"
     CDS             complement(405..605)
                     /locus_tag="ACGE4T_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388079.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
BASE COUNT          243 a          134 c          120 g          234 t
ORIGIN      
        1 taacatttat atgaaaatag tcgaaaggaa atcataatat gtttaagaaa acaaaatctt
       61 tagaatcagg taagaaaaaa attgatctgg atactttaaa gatttcatag ttattgatac
      121 aaaatggaat aaaaaggaca tagaaaatat tctaaatagt aggaacaatt aagtgctatc
      181 acttttgttt cttcaaatga aaagtgacgt cactttttta gtgctggtat atcaaatcct
      241 gctttttctt gcgcgatcaa tagatctaac gcaatacaca taatcttaga ttcacttttt
      301 ccgtacttct gctttagttg ctgcaattct ttttgtctct tttcatctaa tgtcacgggt
      361 aaccgtttat ttcctattgc aattggcatt agtactctct cctttcatcg aaataaccct
      421 atgaaaaata tggctgcaat caacttgaat agtagactca caagggctaa cacaaagcgc
      481 caaaaaatca aacaaaatac tagtattcct aagaatacta cgaagtttaa atgtacagct
      541 gcaccatgac gcataagtat ctcgccaata cacagtacga acaacagagg aatgatttta
      601 ttcatatgga tctccttctt tctcttaggt tatcgggctt tgcccgctta tttcgcacca
      661 cctaagcggc gggggcggtt ggagcgattg gtgcgaaata agaaccattc ccttaatttc
      721 aaggtttttg t
//
LOCUS       DAXWLG010000359          721 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.370, whole genome shotgun sequence.
ACCESSION   DAXWLG010000359 DAXWLG010000000
VERSION     DAXWLG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.370"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            23..691
                     /locus_tag="ACGE4T_002888"
     CDS             23..691
                     /locus_tag="ACGE4T_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8388080.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          191 a          124 c          130 g          276 t
ORIGIN      
        1 ttcaaaagaa aagaggctag aaatgaaaaa attaaattta tttacagaat tgtgcggaat
       61 cattttaggc gcaggaatct acggattagc tgtgacagga atcaatattc catctaagct
      121 ggcagatggt ggggttactg gtattgcttt gcttttgcat aatctttttg gattcgctcc
      181 ttctgtcact tcactgcttt ttaatttacc tttattgctt atctcattgt ttctttttgg
      241 aaaacacgct tttctccgga cgatcgtcgg aacatttgcg ctagttttct ttttacattt
      301 atgggagagc cttcatatac attttgctgt agatagtcta ctgatcaata gtttgatgac
      361 aggtattctt tctggcattg gttgcggtct cgtctttcga ttcggcggaa gcacaggcgg
      421 aacagatatc atttatcaag ccattgaaaa atattttcat attaagattg gcaaaagtct
      481 tttcttgatt acttttggta ttttagttgt ctccttgctt tatttggatc tcacacattt
      541 tctgtataca ttacttagct gttctattct atcttataca ttgaataaag tgaagtatct
      601 ggaaattgga tctgttttgc cccaattgga agcaaaacag actacagaaa catttgaacc
      661 acttgatcct cttgaacagt tagagcactg attcttattt ttcactactt tatttaattt
      721 a
//
LOCUS       DAXWLG010000360          719 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.371, whole genome shotgun sequence.
ACCESSION   DAXWLG010000360 DAXWLG010000000
VERSION     DAXWLG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.371"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..708
                     /locus_tag="ACGE4T_002889"
     CDS             <1..708
                     /locus_tag="ACGE4T_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8388081.1"
                     /translation="GMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKE
                     EVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYEL
                     KIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYN
                     QSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHT
                     TKNMQNQLVEKLGKIEI"
BASE COUNT          307 a          100 c           97 g          215 t
ORIGIN      
        1 ggaatgattt ttaaattcgc caaccgtcga tatggattaa aatataatcc cgtaaatggg
       61 gtttctttag caagaaaacc taagacaaac gaagaaataa aaaaagaaaa agatggttat
      121 ttatcaaaag aagaagttca aatgattgct agtaaacaaa aaagttcaca tcaacagtca
      181 cgttattctt taattactaa atttttgttt ttaactggac tacgttatgg tgaattgata
      241 tctttgactg aatctgatta tgatggtaat aaaattacgg tttctggtac ttatgattac
      301 gagttaaaaa ttaaaacaac aactaaaaat acaggttctt atagaaccat tgaattatca
      361 agtaatgcta aagaaatcat agatcaatta attgaagaaa acaaattaat taagaataat
      421 aaagataaat acattttcat ctctaaaaat ggtaacccga tatctattca agcatataat
      481 caatctttaa gagcagtatc caaagaatta aacatagata aaaaagtttc ttctcacatg
      541 ttaaggcatt cccacatttc tctacttaca gaattaggaa tcccattaaa agccattatg
      601 gatcgtgtag gacatgaaga ttcaaaaaca acattaaaga tttatactca tacaactaaa
      661 aatatgcaaa atcaactagt agaaaaacta ggaaaaatag aaatttaaat tgccccttt
//
LOCUS       DAXWLG010000361          708 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.372, whole genome shotgun sequence.
ACCESSION   DAXWLG010000361 DAXWLG010000000
VERSION     DAXWLG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.372"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(144..305)
                     /locus_tag="ACGE4T_002890"
     CDS             complement(144..305)
                     /locus_tag="ACGE4T_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFF8388082.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            375..708
                     /locus_tag="ACGE4T_002891"
                     /pseudo
     CDS             375..708
                     /locus_tag="ACGE4T_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          258 a          139 c          104 g          207 t
ORIGIN      
        1 tcattgcttg atttcattat tgttaaacaa gaatacaagc aagaaagaac tatagttaat
       61 atgataaaaa ttattaaagt tctatttttc tttcttgtaa tatatgctat agcatttttt
      121 atcactttcc taactactcc tctctatttt cttactccat aactaattgc atccactgga
      181 caagcatttt tacatcgtcc acatctgata cactccagat gattgcaatt ctcaactgaa
      241 tctttattca tttggcaaac ttttttacat tttccacaat taatacactt atccttatcc
      301 tgaatcggct acactagact agacagaaaa aataaggtgt gtagaataat aaaaaacaca
      361 ctggaggatt tttcatgcca agacgtcaac gaagaaccta ttcaaaagaa ttcaaacaac
      421 aaatcgtcga tctctatctc gctggtaagc ctcgcgcaga aattattcga gagtatgagc
      481 ttacgccttc ttctttcgat aaatggatga agcaagcaca atcaacgggc tcattcaaag
      541 aaagagacaa cttaacacca gaacaagcag aattgatcgc actaagaaag aaaaataagc
      601 aactcgaaat ggagaatgat attttaaagc aagcggcgct gatattcgga cgaaaagaca
      661 agtaattgat gccaacaagc ataaatattc catatcagcg atgtgcaa
//
LOCUS       DAXWLG010000362          705 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.373, whole genome shotgun sequence.
ACCESSION   DAXWLG010000362 DAXWLG010000000
VERSION     DAXWLG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.373"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            67..663
                     /locus_tag="ACGE4T_002892"
     CDS             67..663
                     /locus_tag="ACGE4T_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFF8388083.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
BASE COUNT          287 a           75 c          126 g          217 t
ORIGIN      
        1 gagttttttt cgatttttca agttatctaa tgcaattata ttgatatcat taggagctgc
       61 attgttatga atccaacatt gattactaag aaagaattac taaaaaaatt agatatttct
      121 actggtgtat tagctaattt aattagaaat ggcatgccaa aagagggaga aatgtttaat
      181 cttgataaga ttattacatg gcgagaaaat tggtctaaaa acatattagg tgagttagaa
      241 gttggtagag tatatactaa caaagaaatt tcagagaagt ttaaatgttc caaacagggt
      301 ggaatgagaa gatctcatca aactaatact cttgtactgt ttagtgatca aacaggaagt
      361 aatgtttata aagataagtg gttaaatggt attttacaat ataccggtat ggggctaaaa
      421 ggggatcaag tattggataa gaatcaaaat aaagttttag ccaattcaaa aagtaatttt
      481 gtaaagattc atttatttga aacatttaaa ccaaaagaac acacatatct gggagaagta
      541 tatttagcag gacaaatata tactgttaat gaaaaagata gttcgggcaa cagcagaaaa
      601 gtatataaat ttccattagc tttaataaac caagagcaat tgattgaaga taaagatata
      661 taaccaggaa gaaaatcaaa ctagacatat tcgtaattta tctaa
//
LOCUS       DAXWLG010000363          702 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.374, whole genome shotgun sequence.
ACCESSION   DAXWLG010000363 DAXWLG010000000
VERSION     DAXWLG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.374"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>702
                     /locus_tag="ACGE4T_002893"
     CDS             <1..>702
                     /locus_tag="ACGE4T_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFF8388084.1"
                     /translation="SKKNQPKDIKDIVDTRMYKERDDLNYTLIINKDGNYSWRPLVLL
                     HPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILN
                     WWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLG
                     DKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRY
                     RDDYRIFSNNRNDLE"
BASE COUNT          278 a           93 c          121 g          210 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa ca
//
LOCUS       DAXWLG010000364          698 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.375, whole genome shotgun sequence.
ACCESSION   DAXWLG010000364 DAXWLG010000000
VERSION     DAXWLG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.375"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..633
                     /gene="bioD"
                     /locus_tag="ACGE4T_002894"
     CDS             <1..633
                     /gene="bioD"
                     /locus_tag="ACGE4T_002894"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFF8388085.1"
                     /translation="TNCGKSVASILIYEYLKLYFSVNPIIVKPVQTGQELDTIFYQQC
                     DIPQKNIFNFYSLDIEASLHTAGNLTNQKIDLQSLLDQSKALLSDNRPKIFELAGGIL
                     SPINDKETMLDFVKLLNLPIILVVDNYLGSINHTLLTIHLLESKNIEILGLIYNNTES
                     RNYSTELFEYITNNTCIPQIGKIPYLKNLKYVLKSEKEKQNLIREWHLNV"
BASE COUNT          264 a           96 c           96 g          242 t
ORIGIN      
        1 acaaattgtg gtaaatcagt agccagtatt ttaatttatg aatatctgaa actctatttt
       61 tcagttaatc caattattgt aaagccagtg caaacaggac aagagcttga tactattttt
      121 tatcaacagt gtgatatacc tcaaaaaaac atttttaatt tctattcatt agatatcgaa
      181 gcctcactac atactgcagg gaatttaaca aatcaaaaaa ttgacttaca atccttgtta
      241 gatcaatcaa aagctttact aagtgacaat cgacctaaaa tatttgaatt agctggtgga
      301 attttatctc ctatcaacga taaagaaaca atgttagatt ttgtcaaatt attaaacctt
      361 ccaattatct tagttgtaga taattactta ggctcaatta atcatacttt actaactatc
      421 catcttttag aaagcaagaa tatagaaatt ttgggattga tttacaataa tacagaatct
      481 agaaattatt ctactgaatt gtttgaatat attactaata atacttgtat tcctcagatt
      541 ggtaaaattc cttatctaaa aaatttaaag tatgttttga aatcagaaaa agaaaaacaa
      601 aatttaatta gggagtggca tttaaatgta tagaaagagt caaatgattg cgcgaatgtc
      661 attaatgttg gcgctgttaa ttattacagc tagacttg
//
LOCUS       DAXWLG010000365          696 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.376, whole genome shotgun sequence.
ACCESSION   DAXWLG010000365 DAXWLG010000000
VERSION     DAXWLG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.376"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(54..629)
                     /locus_tag="ACGE4T_002895"
     CDS             complement(54..629)
                     /locus_tag="ACGE4T_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFF8388086.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAXWLG010000366          668 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.377, whole genome shotgun sequence.
ACCESSION   DAXWLG010000366 DAXWLG010000000
VERSION     DAXWLG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.377"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(90..359)
                     /locus_tag="ACGE4T_002896"
     CDS             complement(90..359)
                     /locus_tag="ACGE4T_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="HFF8388087.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYK
                     KYPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
     gene            complement(449..>668)
                     /locus_tag="ACGE4T_002897"
     CDS             complement(449..>668)
                     /locus_tag="ACGE4T_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8388088.1"
                     /translation="NLFFTYIGIGIVAMIFFHVFENIGASIGLLPLTGIPLPYISQGG
                     SAILSNFLGLGFMLCCSRYTNEKKGTQK"
BASE COUNT          234 a          114 c           83 g          237 t
ORIGIN      
        1 atttttcata aagataacca ctccttttca aattaaaaac atcctctcta tacgctattt
       61 tattgtatag aaagaatgtt ttgtttttac taaatgtagt aataatattt ggctgttcta
      121 ctactagcat tccaactcat aaggtaacaa gtacgcatag tttgtttacc tctaatattt
      181 actaacattt taactttatt tggcacttta gcaccttttg gatatttttt gtagatatat
      241 cctctttgag cagcagcttc aaccactcca ccattttgag aaaatgaaac agaaggggtg
      301 aaagctaata aactgctaaa caaacacaaa ctagctacca ataattttgt ttttttcaaa
      361 ttatgacact ccttttttaa tgattaactt taaggtatca taatttttat tgaaatagac
      421 attctattca taaagtgtta ttttgaattc atttttgtgt tccttttttt tcgttagtat
      481 atctactaca acacaacata aatcccaacc ccaaaaaatt actaagaatg gcagaaccac
      541 cttgactaat ataggggagt ggaataccag ttagaggtag taatccgata cttgcaccta
      601 tattttcaaa aacatgaaaa aaaatcattg caacaatgcc tataccgata taggtgaaaa
      661 ataagtta
//
LOCUS       DAXWLG010000367          650 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.378, whole genome shotgun sequence.
ACCESSION   DAXWLG010000367 DAXWLG010000000
VERSION     DAXWLG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.378"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..630
                     /locus_tag="ACGE4T_002898"
     CDS             <1..630
                     /locus_tag="ACGE4T_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFF8388089.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKR
                     LVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRN
                     KFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          226 a          116 c          129 g          179 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt
      301 aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agccccctct
      361 attacaagtg cctttaagaa actaaaagaa tacggctttt atcaagggac agaacatcgt
      421 accattaaat acctgaataa tttgattgaa caagaccatc gtccagtaaa gagacgcaat
      481 aaattctatc gaagtttacg cactgcctct accacgatta aaggcatgga agccattcga
      541 ggattatata agaaaacccg aaaagaaggc actctcttcg ggttttcggt ctgtactgaa
      601 atcaaggtat tattgggaat cccagcttaa atcatagata ccgtaaggga
//
LOCUS       DAXWLG010000368          650 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.379, whole genome shotgun sequence.
ACCESSION   DAXWLG010000368 DAXWLG010000000
VERSION     DAXWLG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.379"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..588)
                     /locus_tag="ACGE4T_002899"
     CDS             complement(<1..588)
                     /locus_tag="ACGE4T_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8388090.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTP"
BASE COUNT          196 a          106 c           94 g          254 t
ORIGIN      
        1 tggagttagt tcttctttta ttggtttgag tacaaactga tttatatttg tttgtctata
       61 gcttttagga atatctaata gctctaaaaa atcttcctta gaaaattcag cccatccttg
      121 agttcggtat tgttttagca atctaaacat tgttttagca taactgctat ttaaatctcg
      181 aaattctgtt aaagagaaac gtacccagct ttctagatta ttaagtaaag gcaaagcttt
      241 ttcgtggact tttatatcta cataagggtt atcgacttct ccatgaattt caaaatcagt
      301 aaacattgta aatcgttcta ttgttaatcc accattgctt tttcttccga aacgtaaatc
      361 catcaaatga tcgtatgttc ttgccaaatc ttttataaag acttctcctg cagtctgctt
      421 atacgcactt aaatctttaa gttggctgaa ataaaagcgt attttttgat tacctttatc
      481 gcgcattcta gagacaatag aaaaaaacaa gttcatttct attggggtaa attttcttaa
      541 aggaatggta tttaattctg gttcatactt tacgatttca tttgccattt agttgttact
      601 ccagtctaaa tgattttatt gacatcattt taggacatat tatgtctaaa
//
LOCUS       DAXWLG010000369          633 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.380, whole genome shotgun sequence.
ACCESSION   DAXWLG010000369 DAXWLG010000000
VERSION     DAXWLG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.380"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            130..>633
                     /locus_tag="ACGE4T_002900"
     CDS             130..>633
                     /locus_tag="ACGE4T_002900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388091.1"
                     /translation="MFNQKKEKQFKYAIKKKKGGGGAASFVIGAVFLGIGLISYPTLS
                     FASETTEINQSSEIQSSLTNSVLKESNSEEKQNIDMSEDSSINNDLELDSKATVSGSE
                     NSFDETTNNLETTNLSEDSIEVQSESIIQNEDSTDNDAVQELEMNPHEDNGDDISVEN
                     QMTEATEE"
BASE COUNT          245 a           79 c          111 g          198 t
ORIGIN      
        1 aattttttct aaactgatat actattaata gtatatgacg aagcaagttt attttctttg
       61 ttttcataaa atgaacgatt atttcgtctc tttttgattt ttatactgta ataaataagg
      121 aggaaaatta tgtttaatca aaagaaagag aagcagttca aatatgcaat caaaaagaaa
      181 aaaggtggcg ggggagcagc ttcctttgtc ataggtgcag tatttcttgg aatagggtta
      241 atttcttatc ctacattatc ttttgctagt gaaacaacag agataaatca atcaagtgaa
      301 atccaatcta gtttgactaa ttctgttcta aaagaaagta attcagaaga aaaacaaaat
      361 attgatatgt cggaagatag ttctataaat aacgatctag aactagattc gaaagcaacc
      421 gtttctggat cagaaaattc atttgatgaa actactaaca atctagaaac tacaaattta
      481 tctgaagata gtattgaagt tcagtcagag agtattattc aaaatgaaga ctcgactgat
      541 aatgatgctg ttcaagaatt agaaatgaat cctcatgaag ataacggtga tgatatttca
      601 gttgagaatc agatgacaga agctacagaa gaa
//
LOCUS       DAXWLG010000370          631 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.381, whole genome shotgun sequence.
ACCESSION   DAXWLG010000370 DAXWLG010000000
VERSION     DAXWLG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.381"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            19..>631
                     /locus_tag="ACGE4T_002901"
     CDS             19..>631
                     /locus_tag="ACGE4T_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8388092.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKK"
BASE COUNT          216 a          100 c           95 g          220 t
ORIGIN      
        1 taaaaaggag cctctttcat gcaaactcaa gaagcgatta aaacgtttct cttgaagaaa
       61 aaaagcaata caaaattaga tatttttctt ttcctatctg aacatcgttt ctttattact
      121 acacaatatt tagcagatca tttccatatg tctgaatcaa attttttact atacatcaaa
      181 gaattagaac aagactttga gcgtctcaat ctaacagaac tgcatattga taagcaaaaa
      241 ccttttttaa agttgaattt tgaaggaatt gatccggctt actgctatta tcgtctattt
      301 ggaagatact gcaacgaatc agtcagttat cagatcttga cttctttgtt cagctgtcag
      361 acaaattcga ttatcagttt aagtcagcaa acaaactata gtgcctctta tttgtatacg
      421 aaaatgaaaa agattaatgc atttttagca ttgtacggtt tgtctatctc cttttctaat
      481 aatggcagaa agtctttttc tggaaaagag gtacaaattc aatatgcagc attagatatt
      541 tattggaaca tcttttcaag tactgctaca gaattttatg atgaagatcc gcaagtagtg
      601 gcttttatga tgaatacttt ttttaaaaaa g
//
LOCUS       DAXWLG010000371          630 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.382, whole genome shotgun sequence.
ACCESSION   DAXWLG010000371 DAXWLG010000000
VERSION     DAXWLG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.382"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            483..569
                     /locus_tag="ACGE4T_002902"
     CDS             483..569
                     /locus_tag="ACGE4T_002902"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8388093.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          183 a          148 c          124 g          175 t
ORIGIN      
        1 cttctgtatc cgttgggaga gtaaggctca actgccccct aaaaagggtt ctaaaaaatc
       61 tttaaaaaat aaataaaata aacgcgcgcg cgcgagcaaa atttcccgcc aaaaaatcac
      121 ccacttttta aaataaacag gttcgcgcat gatattaaac tgccaatcta gctccctatg
      181 gtcgctgtaa gaaggagtga tcgctacgct ctcacatttt acttgcagca gtcgccttta
      241 ggctccttcg ccaaaaataa ttcattaggg actcctttgt ggcgtcctga gagctcgtct
      301 aagccgttct aagacaagat tcgataaaaa ctatgtcctt cgttctaaag cgccttacag
      361 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
      421 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
      481 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
      541 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
      601 aagccccaga gaatccgacc tctctggggc
//
LOCUS       DAXWLG010000372          614 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.383, whole genome shotgun sequence.
ACCESSION   DAXWLG010000372 DAXWLG010000000
VERSION     DAXWLG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.383"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            229..>614
                     /locus_tag="ACGE4T_002903"
     CDS             229..>614
                     /locus_tag="ACGE4T_002903"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFF8388094.1"
                     /translation="MACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGY
                     SFGVIGVSFATLLGTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGF
                     YFNLFLTIIFSAIFVFTYKQLIITYL"
BASE COUNT          179 a           85 c          100 g          250 t
ORIGIN      
        1 ataattttaa ctcttacaag tattgttagt atgtctatga tagaggatgc ggttatcacg
       61 tctgagaatg atgatttcat cgaaagattc gttgagaaaa atacctatat ttttaaaatg
      121 tacattaggt tagcgactct ttctattcct atcatttgct gtgcatactc ttttataacc
      181 aaccctgatt ataaaaaatc attagttcta gttccaatat tagttattat ggcatgtttc
      241 agtgggatga caacaaatat cggaaatata tttgcagtat acaataaaac aaatcagtta
      301 ttaatgattt cagtagtgtc aggtctattg agtgttgttg gcacctattt attaggctat
      361 tcttttggag taattggtgt ctcatttgct actttgctcg gtactttagt attatttgga
      421 cttagatatt attttggaag gaaaattgct tattatgcat ttgattggaa gcgaatatcg
      481 ctacttttag ttctctattc cgcaatcgct tttgtatgca ttcatagagg cttctatttt
      541 aatcttttct taacgattat ttttagtgct attttcgttt ttacgtataa acaattaatt
      601 ataacgtatc taaa
//
LOCUS       DAXWLG010000373          606 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.384, whole genome shotgun sequence.
ACCESSION   DAXWLG010000373 DAXWLG010000000
VERSION     DAXWLG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.384"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..221
                     /locus_tag="ACGE4T_002904"
     CDS             <1..221
                     /locus_tag="ACGE4T_002904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388095.1"
                     /translation="SFRINLPNKFIGGFKMSHTLLIKDTLNILDLNINIHFEENCLTK
                     EKYKGQICLIYKGTLQYTPEEYPHLPIY"
     gene            281..514
                     /locus_tag="ACGE4T_002905"
                     /pseudo
     CDS             281..514
                     /locus_tag="ACGE4T_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          229 a          119 c           92 g          166 t
ORIGIN      
        1 aatcctttag aattaatttg cccaacaaat tcataggagg attcaagatg tctcatactc
       61 tgcttatcaa agatacccta aatattctag acctaaatat caatatccat ttcgaggaaa
      121 attgtttaac taaagaaaaa tacaaaggac aaatttgctt gatttacaaa gggactcttc
      181 agtacacacc agaagaatat ccacatcttc caatatatta aaagaacctt cttgaatcat
      241 tgagtgaaag aaaagagatt cgtcactatt tgaggactgt ttaactagag acattagcga
      301 tctgcctgaa acctatacaa ccactttacg aacgtttaaa aattttctgc cacagattca
      361 aaatgcgctt aactgcagct attcaaacag accattggaa tgtttaaaca accacatcaa
      421 agtattaaaa cgcaatacgt atggctttcg cagcttttac aatttcaaac tacggatcat
      481 gattcgtcat ggcaaaacat ttttaaccaa ataaacagaa gaatgtggga taaatctaaa
      541 aatcagtttt gctacagact ctcgagtaaa cggcgggagc agaagcaact ccttcggaaa
      601 taagcc
//
LOCUS       DAXWLG010000374          595 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.385, whole genome shotgun sequence.
ACCESSION   DAXWLG010000374 DAXWLG010000000
VERSION     DAXWLG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.385"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..578
                     /locus_tag="ACGE4T_002906"
     CDS             <1..578
                     /locus_tag="ACGE4T_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8388096.1"
                     /translation="TPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLA
                     HGFTDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIAN
                     YKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYV
                     MMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          206 a          110 c          117 g          162 t
ORIGIN      
        1 acactcctta taaccaacca ggaatcaaat cgcatttatg ggtaacacaa aatatttggg
       61 gcgatcatac agatccttac ggttatcttt ctgaaatggg tgtaagtaaa gaaaaactag
      121 cctatgattt ggctcatggt tttacggatg ataatccaac tacttcggag gataaaccag
      181 taattgatcc aactcgagca ggtgctgcaa attctacgct gacagatgga acaaattacg
      241 cccacattga tcagttcgga gaaatcgaaa acgcaaactt gcatgtagct ggatggcaca
      301 ttgctaatta taaatacgag tatattttca ttatggacta caatactgga aaagaactag
      361 ctagagtaag agctgatggg atttatagac cagacgtaaa tcaagcttat aatactttag
      421 gaaatgttgg ctatcatgta tctttcaata tgcgtgattt ccctaataag aaagtatacg
      481 tcatgatgcg tgcgacaaac gatccagaag gaaatactaa gggtggagca caagattttc
      541 atgacaagcg ttggtattta aatattccac aacgataaaa aatccccccc tcttt
//
LOCUS       DAXWLG010000375          590 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.386, whole genome shotgun sequence.
ACCESSION   DAXWLG010000375 DAXWLG010000000
VERSION     DAXWLG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.386"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(10..246)
                     /locus_tag="ACGE4T_002907"
     CDS             complement(10..246)
                     /locus_tag="ACGE4T_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388097.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            344..523
                     /locus_tag="ACGE4T_002908"
     CDS             344..523
                     /locus_tag="ACGE4T_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388098.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          194 a          104 c          121 g          171 t
ORIGIN      
        1 acaaaaaacc tagtaactaa gggaatggtt cttatttcgc accaatcgct ccaaccgccc
       61 ccgccgctta ggtggtgcga aataagcggg caacacccga taacctaaga gaaagaagga
      121 gatccatatg aataaaatca ttcctctatt gttagtccta tttgttggcg aaatacttat
      181 gcgccatgct gcagctgtgc atttaaactt cgtagtattt ttggcaatac tggtattttg
      241 tttgatcttt tggcgctttg tattagccct tgtgagtcta ctattcaaga tgattgcagc
      301 catatttttc atagggttat ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg
      361 aaataaacgg ttacccgtta cgttggatga aaagagacaa aaagaattgc agcaactaaa
      421 gcagaagtac ggcaaaagtg aatccaggat tatgtgtatt gcgttagatt tattgattgc
      481 ccaagaaaaa gcaggatttg aggtaccagc actcaaaaag tgacgtcact ttttatccta
      541 aaaactaaaa gtgatagcac ttttaattat aagaagttag aatattaatc
//
LOCUS       DAXWLG010000376          583 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.387, whole genome shotgun sequence.
ACCESSION   DAXWLG010000376 DAXWLG010000000
VERSION     DAXWLG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.387"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            200..>583
                     /locus_tag="ACGE4T_002909"
     CDS             200..>583
                     /locus_tag="ACGE4T_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8388099.1"
                     /translation="MYKAANDLFNRLKLEKKELILKYILDEFGRVGYEATSVRKIASK
                     ADLSVGSLYQYFNNKEGMLTVAIEHVFVILDEYVSTINDAPLKLKDRLEIMFEMILKL
                     GKQHPELIIFYNKIPSDKKMITEWVK"
BASE COUNT          220 a           66 c           93 g          204 t
ORIGIN      
        1 tctatcaaca ctttttcttc gtttcaaatc aaaacaaaaa gagactcact tttggtaaaa
       61 tttaagatgt taatcatttt gtgtgtcggt taatttttat caatctaaga tttaattata
      121 gatcattaaa aatgagcggt cgtttttata tactgaatat ggtacgctaa attaaaatac
      181 cttttttgag gagatcatta tgtataaagc agcgaacgat ttatttaatc gattaaaatt
      241 agaaaaaaag gaactcattt taaagtatat tttagatgag tttgggagag taggttatga
      301 agccacttct gttcgtaaaa ttgccagtaa agcagattta agtgtaggat cattatatca
      361 gtatttcaac aataaagaag gtatgttaac agtcgcgata gaacacgttt ttgttatttt
      421 agatgaatat gttagtacta taaacgacgc tcctttaaaa ctaaaagatc gtttagaaat
      481 aatgtttgaa atgattttaa aattaggaaa gcaacatcct gaactcatca tattttataa
      541 taaaattcct agtgataaaa aaatgataac tgaatgggtt aaa
//
LOCUS       DAXWLG010000377          578 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.388, whole genome shotgun sequence.
ACCESSION   DAXWLG010000377 DAXWLG010000000
VERSION     DAXWLG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.388"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>578
                     /locus_tag="ACGE4T_002910"
     CDS             <1..>578
                     /locus_tag="ACGE4T_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8388100.1"
                     /translation="ENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFK
                     IIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAEST
                     DLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLI
                     LILWLRERIYEIGIFLSIGTSKIQIIMQFIFE"
BASE COUNT          234 a           49 c          100 g          195 t
ORIGIN      
        1 aagaaaatga taagaattca attattgttc atgaagaatt tgctaaacaa aacaatctaa
       61 aattaggtga tgaagttgat cttgaattac tagatattga aaaaagtgga aaaataaaaa
      121 gtcataaatt taaaattata gggatctttt ctggtaaaaa acaggaaaca tatacaggat
      181 tatcttctga ttttagtgaa aatatggtgt ttgtagatta ttcaacaagc caagagatat
      241 taaataattc agaaaataat gaaattgcaa ataaaatttt aatgtattct ggtagtgcag
      301 aatctacaga cttagcctta aacaaattga aagagcttaa aattgatgag tcaaagtatt
      361 ttgttgaaaa agattctaat gcgtttgaag agtctttaga gtcagtgagt ggaataaaat
      421 atataataaa agtaatgact tattctatta tgttaggtgg gatggttgtt ctttcattaa
      481 tcttgattct atggttaagg gaaagaattt atgaaatagg tatattctta tctattggaa
      541 catctaagat acaaattata atgcaattta tattcgag
//
LOCUS       DAXWLG010000378          575 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.389, whole genome shotgun sequence.
ACCESSION   DAXWLG010000378 DAXWLG010000000
VERSION     DAXWLG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.389"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            114..311
                     /locus_tag="ACGE4T_002911"
     CDS             114..311
                     /locus_tag="ACGE4T_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFF8388101.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            313..>575
                     /locus_tag="ACGE4T_002912"
     CDS             313..>575
                     /locus_tag="ACGE4T_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8388102.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAG"
BASE COUNT          222 a           56 c          104 g          193 t
ORIGIN      
        1 ttaaattctt attcaggaat tattttgcta cgtttcaaga aggaattgtt ttttgaaatt
       61 taatgaatgt tagaataata gtctaaaaac attaatttag gggtgattag attatgaaac
      121 atttaaaaat tttgtctatt aaagagacac aacttatcta tgggggtacc actcatagtg
      181 gaaaatatta tggaaatgga gtgtattgca ctaaaaataa atgtacggtc gattgggcca
      241 aggcaactac ttgtattgca ggaatgtcta taggtggttt tttaggtgga gcaattccag
      301 ggaagtgcta aaatgaaaaa aaatgctaag caaattgttc atgaattata taatgatata
      361 tctataagta aagatcctaa atattctgat attcttgagg ttttacaaaa ggtatattta
      421 aaattagaaa aacaaaaata tgaattagat cccagtcctt taataaatag attggtgaat
      481 tatctatatt ttactgctta tactaataaa ataagattca ctgaatatca agaggaatta
      541 ataagaaatt tgagtgaaat tggaagaact gctgg
//
LOCUS       DAXWLG010000379          574 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.390, whole genome shotgun sequence.
ACCESSION   DAXWLG010000379 DAXWLG010000000
VERSION     DAXWLG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.390"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>574
                     /locus_tag="ACGE4T_002913"
     CDS             <1..>574
                     /locus_tag="ACGE4T_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFF8388103.1"
                     /translation="TNTLKRWLTALVVGLLSIIAMYYVTDITAHAWAHVPHITEVKKD
                     TSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEIFKSKPISGGSPGYYIEYAH
                     DKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLFIQCFKALRYCFTQPNLVKD
                     KHFFAILMILTIAVSALFITELILVSTIGSF"
BASE COUNT          191 a           91 c          101 g          191 t
ORIGIN      
        1 aactaatact ttaaaaagat ggctaactgc attagtagta ggattgctaa gtataattgc
       61 gatgtattat gtaacagata taactgctca tgcatgggca catgttccgc atattacaga
      121 agtaaaaaaa gatacatctg attcatcagg aacaactagc agcaataaag ttgatttaaa
      181 acgccctgac actgatagcg gggatgtttc taatttaagg tttgaacttt ggaaaagtgc
      241 aattgaaata ttcaagagta agccgatatc cggaggttct ccaggctact atattgaata
      301 tgcacatgat aaactaccat atacattaat gggaaaagat actttaactg ctcataattt
      361 tattttctta gtgatggctg ctacaggagg attaggtcta ttgacattgt taaccttctt
      421 atttatccaa tgctttaaag ccttgcgtta ttgtttcaca caaccaaatc tagtaaaaga
      481 taagcacttt tttgctattt taatgattct tacaatagca gtttcggcat tatttatcac
      541 agaactcatt ttggttagta cgataggttc attt
//
LOCUS       DAXWLG010000380          571 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.391, whole genome shotgun sequence.
ACCESSION   DAXWLG010000380 DAXWLG010000000
VERSION     DAXWLG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.391"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..>571)
                     /locus_tag="ACGE4T_002914"
     CDS             complement(<1..>571)
                     /locus_tag="ACGE4T_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8388104.1"
                     /translation="HIRASLKDAVEEGLIPFDPTRKAVIKGKDSLKPKEDKYLDYDQF
                     KSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDIDFEDQIITINKTWNYK
                     LNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIKNPHNFVFFNLKNGLV
                     SSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT          165 a           94 c           83 g          229 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg t
//
LOCUS       DAXWLG010000381          561 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.392, whole genome shotgun sequence.
ACCESSION   DAXWLG010000381 DAXWLG010000000
VERSION     DAXWLG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.392"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>561
                     /locus_tag="ACGE4T_002915"
     CDS             <1..>561
                     /locus_tag="ACGE4T_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HFF8388105.1"
                     /translation="DKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLADK
                     HPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLL
                     NFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDP
                     VSNQMFSVDWLNEDAKKSFYEGTIKDE"
BASE COUNT          211 a           74 c          102 g          174 t
ORIGIN      
        1 gataagctct attgggaaga aattaaggca agtataagaa tgaatgccac aaacttagat
       61 ggtgatagag aaattcgtta tagtataaat ttacaaaaac atttattgga aataaaaaag
      121 ttagcagata agcatcctaa ttcaggccaa ttgaaaaaag cactttctga gtttaataaa
      181 gaacgcatta ttaaaatcac ttcaaagccg aacgatcttt taccacttat tggtatcctg
      241 gtagatatca tgataaataa cccgggttct ttgccgcagt gtgtcttaat tatagggcag
      301 ttattaaatt tcgaaaatga gagtaaggct agagaaatag ttgaaatgat tataaacaaa
      361 tatgctttaa agccaaatac tgaatttatg atgatatggt ttcaaagatt gaccaccatt
      421 tttggagaaa tgtttgatag tgttttatca tccgaatttg tatctaaaat caaagatcct
      481 gtctctaatc agatgttttc tgtagactgg ctaaatgaag atgctaagaa atctttttat
      541 gaagggacca ttaaagacga a
//
LOCUS       DAXWLG010000382          549 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.393, whole genome shotgun sequence.
ACCESSION   DAXWLG010000382 DAXWLG010000000
VERSION     DAXWLG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.393"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(101..>549)
                     /locus_tag="ACGE4T_002916"
     CDS             complement(101..>549)
                     /locus_tag="ACGE4T_002916"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFF8388106.1"
                     /translation="DYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSE
                     ADALIAVGKGNKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWRE
                     YLNSESGRDQLAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
BASE COUNT          182 a          109 c           70 g          188 t
ORIGIN      
        1 tcaaaaaata taacttaatt ttttatgact ttaaaaagga aatcttctat ctccaaatat
       61 ttactaggat ttgaaagaat agtttagaaa tcatttaggg ctaataatga cttatgattc
      121 ttaatttagc gcttttatat acattattgc ctaaggtagt cacttccttc attgcatgtc
      181 cggacttcca caacacgtaa ggaaatgcga gctgatctcg cccactttct gagttcaaat
      241 actctctcca ccacaaatct agaagattct cagcgattga attatttaaa tcaactaaaa
      301 taaccgtggc ttcagccaat ccaaaattat caggaaatcc ttcttctctg tacttactaa
      361 tttgctctat aatactttcc ttattacctt tgccaactgc aattaatgca tctgcctcag
      421 aatataaaga ttttctttca ctatgtttat gcatacttaa cccaagtttg gaagaactgt
      481 tcagtaatcc ggtaatatca gaagttattg ttacattacc atcaacatac aaagtaaaat
      541 cgtagtcag
//
LOCUS       DAXWLG010000383          542 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.394, whole genome shotgun sequence.
ACCESSION   DAXWLG010000383 DAXWLG010000000
VERSION     DAXWLG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.394"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..375
                     /locus_tag="ACGE4T_002917"
     CDS             <1..375
                     /locus_tag="ACGE4T_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388107.1"
                     /translation="DVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIED
                     TEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVVK
                     FNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          190 a           79 c          106 g          167 t
ORIGIN      
        1 gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa tccagctctt
       61 tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc tgtggtgtct
      121 gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa caagaatgaa
      181 aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt taacgatata
      241 aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca ttatgctgtt
      301 gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt caacaaacca
      361 gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa atacacatcg
      421 gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc gcatcggtaa ttctagcatc
      481 tccagttgtt gtggatgcaa gttctactcc gaaaactaaa aatgataatc aacttgtaat
      541 ta
//
LOCUS       DAXWLG010000384          542 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.395, whole genome shotgun sequence.
ACCESSION   DAXWLG010000384 DAXWLG010000000
VERSION     DAXWLG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.395"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          198 a           69 c           64 g          211 t
ORIGIN      
        1 tccactttta gataattttt aggtctattt aactcatcag acattctatg ctcatttttc
       61 tccaaatttg tttcaactct ttcgtactta attttgcgta tttgaaaatt cttcttttag
      121 agtactttta ttacgatact agtatgatat atatctatag caaacagtta aaatcttcct
      181 aaaataagcg aatagcattc ctaaaaccta taaaattttt agatgaagcg acaagtggac
      241 aattaataaa ctacttgaac atgaaattat ggatcacctt tcaaatttag aaaatggaat
      301 tcttatactt attgcgcaac atttattgat tgcaaaatta tgtgacaaaa aaaactagta
      361 gaagaaagaa taatggcttg ataataaagt actacctatt aaaggtagta ctttatttaa
      421 tttagtataa attaacatac tactatattt taggtttcat attatataaa ttaataatat
      481 actaccattt gttggtagta taatttaaag gtaataagtt attcttttat tactatgttt
      541 gg
//
LOCUS       DAXWLG010000385          533 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.396, whole genome shotgun sequence.
ACCESSION   DAXWLG010000385 DAXWLG010000000
VERSION     DAXWLG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.396"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            74..>533
                     /locus_tag="ACGE4T_002918"
     CDS             74..>533
                     /locus_tag="ACGE4T_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFF8388108.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRP"
BASE COUNT          170 a          105 c           99 g          159 t
ORIGIN      
        1 taaaaaaata tagaaaagac accctgaaat cctttagaat taatttggct accaattcat
       61 aggaggattc aagatgtctc atactcagct tatcaaagat accctaaata ttctagacct
      121 aaatatctat ttcgaggaaa attgtttaac taaagaaaaa tacaaagggc aaatttgctt
      181 gatttacaaa ggaactcttc tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt
      241 cccctctagg atcattcgtt gggggacgac aaccgttcgg ttgttactga atgatgtgtc
      301 tgagtatcgc acttatctgg agctgaagaa acaacgattt aaatgtaagt cttgccaaag
      361 aacattcgta gcggatacct ctgttgctga aaaacactgt ttcattagtc aaaaagttcg
      421 ttggtctgtc attgctcgct taaaagagaa tacttccatg acggaaatcg ctcgtcagaa
      481 aaatatttcc acttcttcgg tctatcgtgt catgaagcga ttctaccgcc cat
//
LOCUS       DAXWLG010000386          512 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.397, whole genome shotgun sequence.
ACCESSION   DAXWLG010000386 DAXWLG010000000
VERSION     DAXWLG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.397"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          212 a           54 c           66 g          180 t
ORIGIN      
        1 catttaaatt ttgaattatt ttttcaaatt agattaatta atatccttat aaaagagcat
       61 aattaatggt ttttacatcg ttatttattg atattaatct catccaaaaa aatgaaagcg
      121 catactttta tgagtgattg aatgtgattt taatacatat accaattaaa agaaaacttt
      181 ttagattttt acttacaaat ttataaaaaa acaagataat tgatactttg attttgcgaa
      241 cattttatct ttccgcctaa ttcagcttta atatcatgat tatatcagta aaaaatcgtt
      301 tttttagcta atttaagcat caaataatta ttcttaatca atgaaaaaat aactaataaa
      361 ttaattttgg gaataggtaa aaacttgttc ggtttctgga taaaaataac atgaaaaagg
      421 aaatataaaa taacatggag cgtgggagga ataaagaaat tttacacgca aggtgcgtag
      481 gaatgtaaag aaacttaaca cgaaacaaaa gt
//
LOCUS       DAXWLG010000387          510 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.398, whole genome shotgun sequence.
ACCESSION   DAXWLG010000387 DAXWLG010000000
VERSION     DAXWLG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.398"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..>510)
                     /locus_tag="ACGE4T_002919"
     CDS             complement(<1..>510)
                     /locus_tag="ACGE4T_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8388109.1"
                     /translation="FIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFVSTK
                     DDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLFMQV
                     YTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKSENI
                     LVYTKKSFDY"
BASE COUNT          195 a           83 c           69 g          163 t
ORIGIN      
        1 gtaatcaaaa gatttttttg tgtaaactaa aatattttct gattttccct ctgcctgtgc
       61 tttagatatt ttaggcaaca taacaatagt aattgatgtt aagactgcca atataatacg
      121 agcaattttt tgagactgat cataataaga taatgctgtt ggatccacaa aatacccaac
      181 tatcgttttg tctaaagttg tgtacacttg cataaacaac tgaggagtca aaataataaa
      241 agctggtttc cacaactgtt taaaaacatt agagttaaag agtggtttac ctacttttcc
      301 gtggatactc aaccagaaaa ctaaacttcc taaaaagtta gtaattgaag taatgaacac
      361 ataaatccac aaatcatctt tagttgaaac aaaaaggatt attaacaata ccgatgataa
      421 tttgataact gtatttctaa taaccacttt cgaaaattct tcaattcctt gaaaaaacca
      481 agaaatatct aatgttagcc ctgcaataaa
//
LOCUS       DAXWLG010000388          509 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.399, whole genome shotgun sequence.
ACCESSION   DAXWLG010000388 DAXWLG010000000
VERSION     DAXWLG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.399"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          228 a           70 c           81 g          130 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactct
//
LOCUS       DAXWLG010000389          506 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.400, whole genome shotgun sequence.
ACCESSION   DAXWLG010000389 DAXWLG010000000
VERSION     DAXWLG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.400"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..505)
                     /locus_tag="ACGE4T_002920"
     CDS             complement(<1..505)
                     /locus_tag="ACGE4T_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388110.1"
                     /translation="MNVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTF
                     YPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKL
                     SVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLD
                     MNILFLLL"
BASE COUNT          195 a           78 c           65 g          168 t
ORIGIN      
        1 atagcaataa aaataagata ttcatgtcta aaaaaaacgg ctttccaaat gatgaaatac
       61 ctaaatctaa cttattaaga aagttgaaat ttttatattc aggaaaccat ttattaattt
      121 catgtgcagc acgtaatgca ttccctggag ttgtcaaaga gaaaattaat tctgctaagc
      181 ttattattga aaatactgat aacttataat tatatcgtcc attgacaatc aaatataaac
      241 taactatact tgttagaaca aaaaaacagg cgttgacttg ttcttgatta gttaaaaata
      301 tcaataaagg aatacttacc caatatattt ttctatttat ttcttctcct cttaagagtt
      361 gaaaaaacgg ataaaaagtt agtaaactaa atgctactgg ccactgatag ttcaaggtgg
      421 ttgctctcca accggtttcc cccattactg tcaatggtat taaacagtat atagagcaaa
      481 ccaataataa atttttaaca tttatt
//
LOCUS       DAXWLG010000390          491 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.401, whole genome shotgun sequence.
ACCESSION   DAXWLG010000390 DAXWLG010000000
VERSION     DAXWLG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.401"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            45..>491
                     /locus_tag="ACGE4T_002921"
     CDS             45..>491
                     /locus_tag="ACGE4T_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8388111.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNL"
BASE COUNT          193 a           58 c          103 g          137 t
ORIGIN      
        1 acatttgtaa aaataaaaaa gatgattttt aggaagtgaa gagagtgcat aaaattagtg
       61 ttatagtacc cgcatataac gtagaaaact atttagaaaa ctgcttagaa tctatttcca
      121 atcagacaca ttcaaatatt gaaataatag tagtcgatga tggttccccg gatcgaagtg
      181 gggaaattgc agaagaatat aaaaagaaag atatgcgatt aaaagtgata catcaagtga
      241 atggtggttt aagtgcggct aggaattctg gtcttgattc tgcaacagga gattttgtgt
      301 gctttattga cagcgatgat tggatagaac cagactatct tgagttacta tattttggta
      361 cgcaaactta tggagcagat atctcggtgc taaaaataaa aaagatttca agtttagaac
      421 aactaaaaga acatacaatt gctgagagaa aatgggaaat atttgataga gaacaagcaa
      481 tgaaaaactt a
//
LOCUS       DAXWLG010000391          477 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.402, whole genome shotgun sequence.
ACCESSION   DAXWLG010000391 DAXWLG010000000
VERSION     DAXWLG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.402"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            41..>477
                     /locus_tag="ACGE4T_002922"
     CDS             41..>477
                     /locus_tag="ACGE4T_002922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388112.1"
                     /translation="MTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTINNIVDLE
                     AINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNAEKKVIL
                     DKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIV"
BASE COUNT          194 a           50 c           77 g          156 t
ORIGIN      
        1 tttcaaaaaa ttcataaaaa tttccggaga atcaaaaaat atgaccattt atccatgttc
       61 tttcaatgta gatgaccaaa atgattatgg agagaaaata gtcaataaaa gcttagagtc
      121 tacgttgaac cttgcttatt taggtacgat taataatatc gtagatttag aagcgattaa
      181 tgaattattg aaaacatgca aaagaaaatt tggaaacaaa ataaatcttt actttattgg
      241 aaaaggtgaa agcaaaacta aattagctga tatttgtgaa aaaaatcata ttgaatttgt
      301 tgactgcggt gttatatatg acaatgcaga aaaaaaagtt atactcgata aatgtcattt
      361 tggactaaat ttatttaagt caaataccat aattggttta agtctgaaat cgattgagta
      421 ttttaggttt ggattaccta taatttcaaa tttatcagga gatacgaaag aaatagt
//
LOCUS       DAXWLG010000392          471 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.403, whole genome shotgun sequence.
ACCESSION   DAXWLG010000392 DAXWLG010000000
VERSION     DAXWLG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.403"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          169 a           90 c           78 g          134 t
ORIGIN      
        1 catcaaaaaa aataaacaat ttttttgaat gaaattacgg gcgaaaataa tcactcaaaa
       61 ttattaataa aaacgaatca actacttttt ctgaagaaac atacaaccgt aaacaacaat
      121 tgttgtttac ggttgtaaat tatcaaacta tatggtgact tgttgcttaa aataggttga
      181 aatttcgttt attttttttg agaaatttta cccacgtact acccatgtac ctatccacgt
      241 attacccatg taccccccca tgacaaatgg gcggaggggc ttgcggcgct tggtattaaa
      301 ggcattatcc gcctcccagg tttctgacca ttttctctca taagtggggt ttttaccgcg
      361 ttgagagaaa atggtcagaa acctaaacga tcggcaaaga aaacatacca agcgccacaa
      421 gcaaaaaaag ttagagtaac ttccagaaaa gaaaaatttt tagaacaact t
//
LOCUS       DAXWLG010000393          468 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.404, whole genome shotgun sequence.
ACCESSION   DAXWLG010000393 DAXWLG010000000
VERSION     DAXWLG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.404"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>468
                     /locus_tag="ACGE4T_002923"
     CDS             <1..>468
                     /locus_tag="ACGE4T_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFF8388113.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAXWLG010000394          464 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.405, whole genome shotgun sequence.
ACCESSION   DAXWLG010000394 DAXWLG010000000
VERSION     DAXWLG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.405"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>464
                     /locus_tag="ACGE4T_002924"
     rRNA            <1..>464
                     /locus_tag="ACGE4T_002924"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          141 a          103 c          131 g           89 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagt
//
LOCUS       DAXWLG010000395          462 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.406, whole genome shotgun sequence.
ACCESSION   DAXWLG010000395 DAXWLG010000000
VERSION     DAXWLG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.406"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            116..310
                     /locus_tag="ACGE4T_002925"
     CDS             116..310
                     /locus_tag="ACGE4T_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388114.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          199 a           58 c           77 g          128 t
ORIGIN      
        1 gaaaaaaact ttcaaattga ttctttttat ttatcataaa gatatagata cataatcatt
       61 ttcacttaca tatatttgaa tattaactaa aaacagcaag aaagaagagg atcaaatgta
      121 ttatgtagaa gtaaaaacaa aaggcgtcaa aaacaaacaa tatgtgaaag gtatcagtaa
      181 cgaatatcct ttacttggtt catggaaaga agctgctcct ttttccaaac catgtgccat
      241 aaaaataaag aacgagctag aaaaagaact gacttgtggt aaagctgttg tagatatcat
      301 cgaaaagtaa atccaatgta tagaaaggaa gatcacacat gaaaagcgac gtacaaattg
      361 ctaagattat agaattagaa gaagaagacg cagtgttgac aattgaaaaa aaataaatgt
      421 ttgtatacca tatgattatt tcaatatgta tcctggaatt gg
//
LOCUS       DAXWLG010000396          458 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.407, whole genome shotgun sequence.
ACCESSION   DAXWLG010000396 DAXWLG010000000
VERSION     DAXWLG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.407"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            5..>458
                     /locus_tag="ACGE4T_002926"
     CDS             5..>458
                     /locus_tag="ACGE4T_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8388115.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT          161 a           69 c          103 g          125 t
ORIGIN      
        1 gtaaatggtc atgaatgcta tttcggcgat gaaaaaaaca aaacagcagc tacatgaagc
       61 ggggtttaag ctgacaccgc aaagagaagc tactctgctt gtgcttttgg aaaatgagaa
      121 agagcattta tctgcagaag aaatcttctt tttagtgaaa aaaaagagtc cggaaattgg
      181 acttgctact gtttatcgaa cccttgaaat cttgacagaa ttgaaaattg tcgataaagt
      241 aagttttaat gatggattag cccgttatga tttgagaaaa gaaggcgcca aacatttcca
      301 tcatcattta ctatgcttga actgcggcac gatcgaggaa gtagaagagg atcttttact
      361 tgaagtggag caagtagtag aagaacgtta tcatttttta gtaaaagatc atcggctaac
      421 gttccatgga atctgtcagg aatgctatga aaaagaaa
//
LOCUS       DAXWLG010000397          453 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.408, whole genome shotgun sequence.
ACCESSION   DAXWLG010000397 DAXWLG010000000
VERSION     DAXWLG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.408"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(296..382)
                     /locus_tag="ACGE4T_002927"
                     /pseudo
     CDS             complement(296..382)
                     /locus_tag="ACGE4T_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          151 a           89 c           75 g          138 t
ORIGIN      
        1 gctaccccgg cctaatctgg caaggggagg aggagatata atgaaatacc tttttgattt
       61 tgtttttgca atcaagccac ttgcagatgt agtgtgcagt tacctctgca aatggcttga
      121 ttgcaaaatt gccaaaaaag acagtaagcc tacccgttaa ttctataata tagaaacgta
      181 aaaaaaaccc catcagcctg cacgctgatg gggtttggtg atataatgaa atacctcttt
      241 gttactcata attataccat aattagtcac gaatgcaacc aatagaaaaa actacttaga
      301 acggtagatc atcgctaata acaatgcatt gtccaccaaa tggattttgt gaagcattaa
      361 atacattgtt attaattgtc gctgctttta actaggcctc tccccttcta ttataaaaga
      421 agatatatat catttcttaa ttacctacta cct
//
LOCUS       DAXWLG010000398          452 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.409, whole genome shotgun sequence.
ACCESSION   DAXWLG010000398 DAXWLG010000000
VERSION     DAXWLG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.409"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..>452)
                     /locus_tag="ACGE4T_002928"
     CDS             complement(<1..>452)
                     /locus_tag="ACGE4T_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8388116.1"
                     /translation="YKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKITKELIK
                     QVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTGAKNW
                     FYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKT"
BASE COUNT          175 a           58 c           69 g          150 t
ORIGIN      
        1 agttttaagt aaataaatcc aatcatcttt cgtattacta gtaatagatt tttgctgata
       61 attgacggtt attttagcta gcataagggt gtatcctatt ttgactaact cagtaggttg
      121 aaacgaaaaa ccagcaatat aaaaccaatt ttttgctcca gttaagactg ctaattgtgg
      181 attatagaat ttcaagagaa gtaataacat tatgatgcat aatccgtaaa atataggagt
      241 taatttccac aaaattttag gattaaaaaa ataaatagtt attgctgaag tgaacccaat
      301 actataccac aatacttgtt taattaattc ttttgtgatt tttgaattag tgtaagagtc
      361 gtgatataaa gcaacatata aagaaagtaa tccaatgata catagcagaa aaactggcaa
      421 taaaagccca taattcaact gatttttgta ga
//
LOCUS       DAXWLG010000399          446 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.410, whole genome shotgun sequence.
ACCESSION   DAXWLG010000399 DAXWLG010000000
VERSION     DAXWLG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.410"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            127..>446
                     /locus_tag="ACGE4T_002929"
     CDS             127..>446
                     /locus_tag="ACGE4T_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388117.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYK"
BASE COUNT          163 a           52 c           77 g          154 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaaca
//
LOCUS       DAXWLG010000400          439 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.411, whole genome shotgun sequence.
ACCESSION   DAXWLG010000400 DAXWLG010000000
VERSION     DAXWLG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.411"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(80..364)
                     /locus_tag="ACGE4T_002930"
     CDS             complement(80..364)
                     /locus_tag="ACGE4T_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388118.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          147 a           83 c           65 g          144 t
ORIGIN      
        1 ttttattttg ctttttctta cgaaaaaatg acaaagaaaa aagtttgtga catgactttt
       61 gtcacaaact ctcgtattct taaccaaaat attgatgaaa tacttgtgta ataactggta
      121 gaaaataaat aatgatcata gccgctacca ccaccaacaa ttgttttcgc tgtttctttc
      181 gctttagcat gcttgtcgtt ttatgaaaaa aataagtagc gcttttagct cctgcagtca
      241 aagtcgataa aatcaacgtt ggtgaatatg ctgtttgatt gtgcgtcaat gacaacaact
      301 tcgtcccgta atcaggatac tttagaaatg cttggtacac agccacaatc gtgattgctc
      361 ccataagata atttaaaaat gaatgcactt ttttcaaata aaaaaactcc ttgctatcta
      421 tataaaatca gcctacccc
//
LOCUS       DAXWLG010000401          434 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.412, whole genome shotgun sequence.
ACCESSION   DAXWLG010000401 DAXWLG010000000
VERSION     DAXWLG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.412"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..>434)
                     /gene="scm"
                     /locus_tag="ACGE4T_002931"
     CDS             complement(<1..>434)
                     /gene="scm"
                     /locus_tag="ACGE4T_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8388119.1"
                     /translation="VNIDAEVLTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYN
                     DRSNHETEDFSDANISWNFGNEMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNV
                     ILDAATNRWDPNEIFISDNGTDTTDTSDSTDTSTTSDSSDSS"
BASE COUNT          118 a           77 c           86 g          153 t
ORIGIN      
        1 tgatgaatct gagctatcac ttgtggtaga tgtatctgta ctgtcgcttg tgtctgtcgt
       61 atctgtacca ttgtcgctga taaatatttc gttaggatcc caacgattgg tagctgcatc
      121 taaaatcaca tttgttccta taatcgttcc gtcttggttt tgagtaatgc ggatagtagc
      181 attaggcgca aggattgttc cttgaaaagg agcactgatt gtcaaatcag tcatctcatt
      241 tccaaaattc caagagatat tcgcatccga aaaatcttct gtttcatgat ttgaacgatc
      301 attgtatttg attggtgact gtacgttcaa tgcagaagaa ctgttgatga cattaaatac
      361 aactacttga tcatttggat taatgatttg aagtggcgta ttcatcgtta acacttctgc
      421 atcgatattt acca
//
LOCUS       DAXWLG010000402          428 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.413, whole genome shotgun sequence.
ACCESSION   DAXWLG010000402 DAXWLG010000000
VERSION     DAXWLG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.413"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(170..>428)
                     /locus_tag="ACGE4T_002932"
     CDS             complement(170..>428)
                     /locus_tag="ACGE4T_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFF8388120.1"
                     /translation="ASDNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSK
                     DYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          130 a           84 c           59 g          155 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgct
//
LOCUS       DAXWLG010000403          415 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.414, whole genome shotgun sequence.
ACCESSION   DAXWLG010000403 DAXWLG010000000
VERSION     DAXWLG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.414"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..339)
                     /locus_tag="ACGE4T_002933"
     CDS             complement(<1..339)
                     /locus_tag="ACGE4T_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8388121.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAXWLG010000404          412 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.415, whole genome shotgun sequence.
ACCESSION   DAXWLG010000404 DAXWLG010000000
VERSION     DAXWLG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.415"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..342
                     /locus_tag="ACGE4T_002934"
     CDS             <1..342
                     /locus_tag="ACGE4T_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8388122.1"
                     /translation="KSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAP
                     IILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKL
                     KKVNKMNLEVE"
BASE COUNT          172 a           39 c           77 g          124 t
ORIGIN      
        1 aaatcagcag atgaaagtat cttgtttgaa ctaggtttag ataaaaaaca aataaaaaga
       61 aatgttatga aattatctgg tggacagcaa caaagagtag ctattgctag agcattggta
      121 tcagatgctc caataatact agctgatgag cctactggta atctagacag tgttactgct
      181 ggagaaataa ttaatatatt aaagacatta gctaaagata gaaataaatg tgtaatagtt
      241 gtaacacata gtaaggaagt agcagattct gcggatatca ttttagaact aagtggtaaa
      301 aagttgaaaa aagtaaataa aatgaatttg gaggttgaat aatgataaaa aatgcatttg
      361 cttatgtaac tagaaaaagt ttaaaatcat taattatcat attagttatt tt
//
LOCUS       DAXWLG010000405          404 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.416, whole genome shotgun sequence.
ACCESSION   DAXWLG010000405 DAXWLG010000000
VERSION     DAXWLG010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.416"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            71..>404
                     /locus_tag="ACGE4T_002935"
     CDS             71..>404
                     /locus_tag="ACGE4T_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388123.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSN"
BASE COUNT          178 a           56 c           60 g          110 t
ORIGIN      
        1 tatataaaaa gccttcgggc tttttctttt aacaattaat aggaacgtat attcgaaagg
       61 aatagaaata atgtggttag aaaaaataaa tgacaaaaaa tttaagtaca ccgaacgtta
      121 tacggatccc ttaactgaaa aaaagagaaa agtgtccgtt actttatcta gcaattcacg
      181 ccaagcttgg aatcaagcta acttactttt gaatgaaaaa atcgcagaaa aaataaaacg
      241 aaatgaagaa ttaccactaa cttttggaga attaaaaact aaatgggatg aaaaatacaa
      301 gccaactgta aaagaaagtt cctatcgaac aactcaggtt tatctatcat taatttcaaa
      361 atatataaaa gatgatgtac ttgtaaaaaa cgttaatagt aatc
//
LOCUS       DAXWLG010000406          403 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.417, whole genome shotgun sequence.
ACCESSION   DAXWLG010000406 DAXWLG010000000
VERSION     DAXWLG010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.417"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            153..383
                     /locus_tag="ACGE4T_002936"
     CDS             153..383
                     /locus_tag="ACGE4T_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388124.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          154 a           52 c           60 g          137 t
ORIGIN      
        1 tataagcatt tttaaaagat tttatatttt tgtatgtcat tcatatatca atagctaact
       61 taatatcagt caaattatgc cccttttttg cccctcaaat atcgctttac aacaagaacg
      121 taagttcgta taatcgtttt gaggtgattt ttatgatgga agaatttatt agaaaaaata
      181 tcagtgatga atatgcagac ttttatgatc aaagcagtaa aaaagataaa ttccagatgg
      241 atgtttcaat cttagctata ttagcttttt ccgaaaataa acaacctgta actgcaaaaa
      301 aagaaacagt attctctgaa ggcaaaataa aaactcgata tatattagag gtagaaacta
      361 attttaaaaa cagatcggag taatggttat gctttttaat gag
//
LOCUS       DAXWLG010000407          398 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.418, whole genome shotgun sequence.
ACCESSION   DAXWLG010000407 DAXWLG010000000
VERSION     DAXWLG010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.418"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>398
                     /locus_tag="ACGE4T_002937"
     CDS             <1..>398
                     /locus_tag="ACGE4T_002937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388125.1"
                     /translation="GFMGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQ
                     LNQMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSC
                     ITGNYDSIKEPDFSHPLVDYYLYTDNVITS"
BASE COUNT          168 a           50 c           68 g          112 t
ORIGIN      
        1 ggatttatgg gaagacaaga attatatagt ttagctatca atgaattgga aaaaagaaat
       61 cttgaaatac atgaattaaa tcaaaaaaat aattctattt taggcatgaa aatacgaaat
      121 aaaattaatc aacttaacca aatgaaaaaa cgagataatc tgttgccagc aaaatatacc
      181 cttaaatcga tgaccggtaa ggggcctatt aaagactatt caaacagaga gaatcgcagt
      241 gatgaactaa gaaaaaaaga gttaactaat caaggaacac ctaaaaggat tgctgtgtat
      301 agttgtatca ctggtaatta tgattctatt aaagaaccgg atttttctca tccattggta
      361 gattactatt tgtatacaga taatgtgatt acaagtga
//
LOCUS       DAXWLG010000408          392 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.419, whole genome shotgun sequence.
ACCESSION   DAXWLG010000408 DAXWLG010000000
VERSION     DAXWLG010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.419"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(34..252)
                     /locus_tag="ACGE4T_002938"
     CDS             complement(34..252)
                     /locus_tag="ACGE4T_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388126.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          139 a           70 c           70 g          113 t
ORIGIN      
        1 cttgtctttt gaataattag cagtcaatcc ttattatgat tgcctggatc tgtagtaggg
       61 ctcatgatag aaatgatctt catgaattct gcgccgctca gattgacggt acttcttttc
      121 atcccacaac atgaaccaca aagtaaataa cggaacaaac acaagtaaag ctggacgtat
      181 gccgaaatta ccaataataa tacctaaaaa caaaacagtt aatactaagc caagacgtct
      241 cattgatttc atgaattctc acctctttaa tttatgcgaa caattgttcg tatatttagt
      301 atacgaatat ttgttcggtt tgtaaagagg gaagtgataa aaatttgaaa gaagaagaac
      361 ccgggcgaaa aaagagtttg agaccaacgt ct
//
LOCUS       DAXWLG010000409          390 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.420, whole genome shotgun sequence.
ACCESSION   DAXWLG010000409 DAXWLG010000000
VERSION     DAXWLG010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.420"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          126 a           53 c           56 g          155 t
ORIGIN      
        1 atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa tgtttttaac
       61 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tatattatgc
      121 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      181 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      241 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      301 ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga gtaattaatt
      361 agatgtttat tttataggac tgatactata
//
LOCUS       DAXWLG010000410          387 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.421, whole genome shotgun sequence.
ACCESSION   DAXWLG010000410 DAXWLG010000000
VERSION     DAXWLG010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.421"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            32..327
                     /locus_tag="ACGE4T_002939"
                     /pseudo
     CDS             32..327
                     /locus_tag="ACGE4T_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          132 a           56 c           68 g          131 t
ORIGIN      
        1 aggaaatgaa attctagata ttttttgcta catagagtta ctttcaatat tttttaacgt
       61 aattaggcag aagtcactca tcctctatag gtttgtgatg aaattgccat tgatccattt
      121 aagtaagagc aaaaaactgg aggtttatca ggtaattggt ctaatggatt taagtatgcg
      181 gtcaccgtgt atagagttgc ttatgagatt caagaaaata aagttattcc agtattgcta
      241 tatggaacac ataacaattt ttatgaatta gccgagaaca ctcgcaactt tattcgtgta
      301 gatgaatcgg cttcggttga gttataactt tagctatatc aatcaaccga ccgacatatg
      361 ttaaatctat atataataaa aatacct
//
LOCUS       DAXWLG010000411          386 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.422, whole genome shotgun sequence.
ACCESSION   DAXWLG010000411 DAXWLG010000000
VERSION     DAXWLG010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.422"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..242
                     /locus_tag="ACGE4T_002940"
     CDS             <1..242
                     /locus_tag="ACGE4T_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388127.1"
                     /translation="RNAGIGFRTKRSTSSEKKWVYSQTIFYGGVISISLLSSTLYSFN
                     VIDVSMSNFISIIGILISAIITQLLLVFEEKSKNN"
BASE COUNT          125 a           50 c           62 g          149 t
ORIGIN      
        1 gtagaaatgc agggataggt tttagaacca aacgaagtac gtcttctgaa aaaaagtggg
       61 tatattcaca gactattttt tacggagggg ttatttcaat cagtttactt tcttcaacac
      121 tttattcttt taatgttata gacgtttcca tgtcaaattt tatatctatt attggaatcc
      181 ttatttctgc aattattaca caactacttc ttgtgtttga agagaagtca aaaaataatt
      241 aaagcaatta tgccttttag tacaggaatt attacttctt acaaatgtat agcgaatgtg
      301 aagcctgctg ttttaattgt tctagaaaaa cttttgagtg aacaattatt ttatgttagg
      361 atgaattaat ttctctagag tttata
//
LOCUS       DAXWLG010000412          359 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.423, whole genome shotgun sequence.
ACCESSION   DAXWLG010000412 DAXWLG010000000
VERSION     DAXWLG010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.423"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..152)
                     /locus_tag="ACGE4T_002941"
     CDS             complement(<1..152)
                     /locus_tag="ACGE4T_002941"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8388128.1"
                     /translation="MSTNKKDNKKEEIIFTAMKHFSKFGYEKTKLLDIANEAKTSTTT
                     VYSFFKT"
     gene            complement(157..>359)
                     /locus_tag="ACGE4T_002942"
     CDS             complement(157..>359)
                     /locus_tag="ACGE4T_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8388129.1"
                     /translation="EKEISTKIDPVIYTFIIDTMMLSGELLQNTQYQEIKAKVFLEDS
                     IKSSLILKENVIETLTVLAKGQ"
BASE COUNT          111 a           48 c           43 g          157 t
ORIGIN      
        1 gttttaaaaa aactatatac agtagtagta ctagttttag cttcattagc aatatctaaa
       61 agctttgttt tttcataacc aaatttagaa aaatgtttca ttgcggtaaa tataatttct
      121 tcttttttat tatctttctt gtttgttgac ataaatttat tgcccctttg caagtacagt
      181 tagcgtctcg ataacatttt cttttaatat taatgaactt tttatagagt cttctagaaa
      241 aactttggct tttatttctt gatattgtgt attttgaagc agctctccag ataacatcat
      301 tgtatcaata ataaatgtat agataactgg atcaatttta gtactaattt ctttttcat
//
LOCUS       DAXWLG010000413          355 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.424, whole genome shotgun sequence.
ACCESSION   DAXWLG010000413 DAXWLG010000000
VERSION     DAXWLG010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.424"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            83..>355
                     /locus_tag="ACGE4T_002943"
     CDS             83..>355
                     /locus_tag="ACGE4T_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8388130.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQ"
BASE COUNT          125 a           60 c           59 g          111 t
ORIGIN      
        1 agaactatag aattccttaa aacttgtcca ttatttcaaa atttgttata gtctttttat
       61 tcaaagcgta aaaggagatc ctatgttaaa agaaaaaatg aaaactctcc ccatgctgcc
      121 gggtgtctat cttatgaaag accgtgatgg taaggtcatc tatgttggta aagcaaaacg
      181 gctcaaaaat agagtaagca gctattttca tcagaataaa cagcattcaa aaaaagtatt
      241 acgtatgatt catcatataa cagattttga tttcgtcgta gtagacacgg aattagatgc
      301 tttattgctc gagtgtcaat tgatccaaca ttaccgtcct ttttataacc gacaa
//
LOCUS       DAXWLG010000414          353 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.425, whole genome shotgun sequence.
ACCESSION   DAXWLG010000414 DAXWLG010000000
VERSION     DAXWLG010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.425"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          112 a           57 c           44 g          140 t
ORIGIN      
        1 taccttattt cttattatat cagtatttct ctgtgacaca taagggtgta acataattat
       61 tttaaacgaa atgactacaa gtagtcattg aagcaaatct ccataagaca tttgtaaaat
      121 cttttaaaaa gaagcgtcga aagaaggaac ttttcttcaa cgcttctttt attgactcaa
      181 ttttcaatat tacttgaacg aataatctga tttctaaact ctcgaaaaac ttcttgtggt
      241 tgataatagt ctggatctat ttcaaatgtt tctgagaata attcattgaa tttttctact
      301 gaaataatga ttttaccatt cttctctcga ctgttttcta ttatttctgc ttc
//
LOCUS       DAXWLG010000415          337 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.426, whole genome shotgun sequence.
ACCESSION   DAXWLG010000415 DAXWLG010000000
VERSION     DAXWLG010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.426"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          123 a           61 c           33 g          120 t
ORIGIN      
        1 tgacaaagag cttctttttt gaatactata ttcgttctgt ttttcatctt caaatacgaa
       61 ctcatccgca gaaatatttg tcacaactcc atgaatcttc ttttcattac cacctatata
      121 ccacaggatt cctacacaga aacaacaaaa aaatagcata aaataggttt tctttatttt
      181 atatccctcc ttttagatta aaagctcaca gaaaacgctt tctaaaaaga caatagaata
      241 atcataattt ttcattttgt attatttata caaataatac gcatgttact gaaaaatgat
      301 atggttccct actttttgag ataagttaaa taaaatc
//
LOCUS       DAXWLG010000416          335 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.427, whole genome shotgun sequence.
ACCESSION   DAXWLG010000416 DAXWLG010000000
VERSION     DAXWLG010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.427"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT           97 a           56 c           65 g          117 t
ORIGIN      
        1 ttttgagtta taataattca tgaagagagg taattcactt atttccccaa gctgaaaagc
       61 ctgatattac ttcttttcag gcagtgctgc taatattcaa ttacactttt ttgtatattt
      121 ttagttgttt taaatttaat tgtctgatat gcttgtgatg aattgatttt cgttctgcac
      181 ttttggacga taaattgtga taattgaaaa tacaaaaaaa gaaaggcccc tgtaaggctc
      241 gggagactgg taatctccgt cagtgtccta caggaaagca ttaagatgac gccttaatgc
      301 gtgcccttca gtgttacggt attacccgca acgga
//
LOCUS       DAXWLG010000417          334 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.428, whole genome shotgun sequence.
ACCESSION   DAXWLG010000417 DAXWLG010000000
VERSION     DAXWLG010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.428"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>334
                     /locus_tag="ACGE4T_002944"
     CDS             <1..>334
                     /locus_tag="ACGE4T_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8388131.1"
                     /translation="SEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDI
                     ETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENS
                     NGILRRNGL"
BASE COUNT          101 a           67 c           60 g          106 t
ORIGIN      
        1 ttctgagttt ggtcaccttg aaggtgatac gatcgttggc attcatcata aaagtgccgt
       61 cattacttta gttgaaagat tatctaaagt cattatcacg attaaaccca acggccgtaa
      121 ggcattagat attgaaactg cccttaatca atggttttct cgcttcccta aaaacttctt
      181 taaatctatt acgtttgact gtggaaaaga attttctaac tggaaagcca ttagtaacca
      241 acatgatatt gatatatatt ttgcggaccc tggaacacct tctcaacgcc cattaaacga
      301 gaattctaac gggattctgc gtcgtaatgg actg
//
LOCUS       DAXWLG010000418          327 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.429, whole genome shotgun sequence.
ACCESSION   DAXWLG010000418 DAXWLG010000000
VERSION     DAXWLG010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.429"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          103 a           62 c           79 g           83 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccaatgagaa aaagcatgga atgcgctgca tcaggtatcg aggcttagaa
      241 aaaaatcagg tgcatacgat gcttgttttt gccgaaatga gtctgaaaaa attggtcact
      301 tagcagtggg agcatatgtc ttcatat
//
LOCUS       DAXWLG010000419          323 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.430, whole genome shotgun sequence.
ACCESSION   DAXWLG010000419 DAXWLG010000000
VERSION     DAXWLG010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.430"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACGE4T_002945"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACGE4T_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFF8388132.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAXWLG010000420          309 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.431, whole genome shotgun sequence.
ACCESSION   DAXWLG010000420 DAXWLG010000000
VERSION     DAXWLG010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.431"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            10..>309
                     /locus_tag="ACGE4T_002946"
     CDS             10..>309
                     /locus_tag="ACGE4T_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFF8388133.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT          110 a           34 c           56 g          109 t
ORIGIN      
        1 agtataaaaa tgaacaaaca taatagaaaa ataacgaata acaaagaact gatcttgtta
       61 ctaataggga tttggggtat ctttttactc tttaatatgt atatgccgac tatgagagcg
      121 gatgacgtag tttatgcaag tagattagat gaacttggct atttgggtgc ttcgatagaa
      181 cattataaga catggagttc aagaattatt atagaactat tcttaatgtt cttttctaaa
      241 cactttatgc tatggaaatt attaaattct acgattatgt taggaattgt agtattactt
      301 tgcaagtat
//
LOCUS       DAXWLG010000421          308 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.432, whole genome shotgun sequence.
ACCESSION   DAXWLG010000421 DAXWLG010000000
VERSION     DAXWLG010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.432"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          105 a           52 c           47 g          104 t
ORIGIN      
        1 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
       61 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
      121 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
      181 cgggctacct ttttaggtag cctactttaa tctttgtatc tttctggatc aacgaaagta
      241 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata
      301 tcaaacaa
//
LOCUS       DAXWLG010000422          307 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.433, whole genome shotgun sequence.
ACCESSION   DAXWLG010000422 DAXWLG010000000
VERSION     DAXWLG010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.433"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          134 a           42 c           43 g           88 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaatt
//
LOCUS       DAXWLG010000423          305 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.434, whole genome shotgun sequence.
ACCESSION   DAXWLG010000423 DAXWLG010000000
VERSION     DAXWLG010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.434"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            33..>305
                     /locus_tag="ACGE4T_002947"
     CDS             33..>305
                     /locus_tag="ACGE4T_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388134.1"
                     /translation="MIILCFENKGYFFEISDIAGFVEVERKKQCSSATLNTYDYVLIN
                     IVKQLSEQQKKLIYLINSKNFNTTLSNLIEMDAKISDYLFFLINCRF"
BASE COUNT          116 a           30 c           46 g          113 t
ORIGIN      
        1 tcaaagaaaa cttattttaa taatttgaga ggatgattat attgtgtttt gaaaataaag
       61 ggtacttttt tgaaatctca gacattgctg gatttgttga agttgaaaga aagaaacagt
      121 gtagctcagc tacattaaat acttatgact atgttttaat taatattgta aaacaattga
      181 gtgagcagca aaaaaaattg atttacttaa taaactcaaa gaatttcaat actacactaa
      241 gcaatttaat tgaaatggat gccaaaattt ctgattatct ttttttcttg attaattgta
      301 gattt
//
LOCUS       DAXWLG010000424          299 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.435, whole genome shotgun sequence.
ACCESSION   DAXWLG010000424 DAXWLG010000000
VERSION     DAXWLG010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.435"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..>299)
                     /locus_tag="ACGE4T_002948"
     CDS             complement(<1..>299)
                     /locus_tag="ACGE4T_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388135.1"
                     /translation="KIEFIDSDINVFFYSKEKLADFSNIAEMIERLKDFMISVWDVAM
                     LADESNYTSSIYRLEDSNWIEISLELKTLPNSVNYIIRDITSEAQQLVNYMHGHE"
BASE COUNT          105 a           49 c           33 g          112 t
ORIGIN      
        1 tttcgtgtcc atgcatgtaa ttaactaatt gttgagcttc agaagttata tctctaatta
       61 tatagttaac ggaattagga agtgtcttta actctaatga aatttctatc caattactat
      121 cttctaatct gtatatgctc gaagtataat ttgattcgtc agcaagcatc gctacatccc
      181 aaacactgat cataaaatct tttaaacgtt caatcatttc agcaatattg ctaaaatcag
      241 ctaatttttc tttagaataa aaaaaaacat ttatatcaga atcaataaat tctattttt
//
LOCUS       DAXWLG010000425          294 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.436, whole genome shotgun sequence.
ACCESSION   DAXWLG010000425 DAXWLG010000000
VERSION     DAXWLG010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.436"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(139..>294)
                     /locus_tag="ACGE4T_002949"
     CDS             complement(139..>294)
                     /locus_tag="ACGE4T_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFF8388136.1"
                     /translation="SRTNIYLSDCLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNR
                     HLTEKIS"
BASE COUNT           99 a           41 c           37 g          117 t
ORIGIN      
        1 cggaataata acttatgtac tttttaattt tgttaagtaa tatatctagt tatatctagt
       61 tatatctagt taccttttct ttgaaaaaaa cataaatcag ctaataaata tgtgagacaa
      121 agctgccatc cataaatact agctgatttt ttcagttaaa tgtctatttt tttctctgat
      181 gatggtattt ccaaaatatt cccagtcatc tggagtcggt ggatttttcc aaattatctg
      241 ttctttatta aatttttcaa gtaaacaatc agataaataa atattcgtac gaga
//
LOCUS       DAXWLG010000426          269 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.439, whole genome shotgun sequence.
ACCESSION   DAXWLG010000426 DAXWLG010000000
VERSION     DAXWLG010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.439"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..190)
                     /locus_tag="ACGE4T_002950"
     CDS             complement(<1..190)
                     /locus_tag="ACGE4T_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8388137.1"
                     /translation="MELQITLEAARVMMGYSLKEAAKLFDVHHQTLANWEQDPNKMKQ
                     KYVQLIPEIYHFPTANIFF"
BASE COUNT           75 a           42 c           36 g          116 t
ORIGIN      
        1 caaaaaaaat atttgctgtt ggaaaatggt atatttctgg tattaattgc acatattttt
       61 gcttcatttt attcggatct tgttcccaat ttgctaaggt ttgatggtga acatcaaaga
      121 gttttgctgc ttcttttaaa gaatatccca tcataactct agctgcttcc aaagtaattt
      181 gtaattccat ttttattcct ccttcgttta tgtaaattat tttatactag tttatactac
      241 cagtcaacga tttttgacgt tttttaaat
//
LOCUS       DAXWLG010000427          263 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.440, whole genome shotgun sequence.
ACCESSION   DAXWLG010000427 DAXWLG010000000
VERSION     DAXWLG010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.440"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..216)
                     /locus_tag="ACGE4T_002951"
     CDS             complement(<1..216)
                     /locus_tag="ACGE4T_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8388138.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT           68 a           47 c           51 g           97 t
ORIGIN      
        1 aaaaatgatt tcggttctcc cacaacgtcg cttattttct ttgtattgtt caaagtattc
       61 aagtgctgtt ccaccacatt tgagaaagtt atagacattg taaattgttt ggcgtccacg
      121 tttaagctgc ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat
      181 tacaagctcg tttggtgtaa gatgggtata agtcatttat gttcactctc cttgtatgct
      241 ttagcgggta ttacaatttg agt
//
LOCUS       DAXWLG010000428          259 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.441, whole genome shotgun sequence.
ACCESSION   DAXWLG010000428 DAXWLG010000000
VERSION     DAXWLG010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.441"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          111 a           27 c           46 g           75 t
ORIGIN      
        1 actaaaaatt ataaaaaata gtgttgacgc acttttgatg tcatgttata ataacgaagt
       61 cgtttggatg agatagctaa taaagcttga ttttatcgaa aaaaacttcg aaaaagttat
      121 tgacaaataa caataaagat ggtatgataa tcaagtcgct gctaatgagt ggcaaagacg
      181 tttagaaaaa ataaacaaag aagttgttga cattctcttc tgaatctgat aagattaaag
      241 agttgttaaa acaaaaaga
//
LOCUS       DAXWLG010000429          252 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.443, whole genome shotgun sequence.
ACCESSION   DAXWLG010000429 DAXWLG010000000
VERSION     DAXWLG010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.443"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT           89 a           40 c           52 g           71 t
ORIGIN      
        1 ctaacttctg tcactttaaa aaagggactg tgacagaagt gaaaatagac aaaaaatatc
       61 cgaacaagag actaggatag cagaaaaact atcgttttgc aacgaggacc aactggaccg
      121 taggagcaat tctcttattg ttcggatatt tcgcttgttc aaggacttat gtctgtagtg
      181 cccttttttg gtaggaaaaa tggtagggaa ccatatcatt tttaatgaaa atcaagcatt
      241 tcaaaagttg aa
//
LOCUS       DAXWLG010000430          250 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.444, whole genome shotgun sequence.
ACCESSION   DAXWLG010000430 DAXWLG010000000
VERSION     DAXWLG010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.444"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..232)
                     /locus_tag="ACGE4T_002952"
     CDS             complement(<1..232)
                     /locus_tag="ACGE4T_002952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8388139.1"
                     /translation="MEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSFNPRIF
                     DSFEVHEDILVFIDKFYPELSINVRTRYVYEAI"
BASE COUNT           84 a           40 c           41 g           85 t
ORIGIN      
        1 ttattgcttc atagacatag cgagttctaa cattaattga taattccgga taaaacttat
       61 ctatgaatac tagtatatct tcatggactt caaaagaatc aaaaattcta ggattgaagc
      121 ttcctctcag tatactttta gggctttggt aatagtgata tttagcagat gaattgacag
      181 caactttttc ggaatgatcg atcaacaaat aagtagtagc cttatcttcc attaatttac
      241 ccagtgggaa
//
LOCUS       DAXWLG010000431          245 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.445, whole genome shotgun sequence.
ACCESSION   DAXWLG010000431 DAXWLG010000000
VERSION     DAXWLG010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.445"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>245
                     /locus_tag="ACGE4T_002953"
     CDS             <1..>245
                     /locus_tag="ACGE4T_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388140.1"
                     /translation="CGIIFMFVIVLGIVVLLITVKIVDYFANTPEEQIQRKLPEFERV
                     PPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK"
BASE COUNT           96 a           30 c           44 g           75 t
ORIGIN      
        1 atgtggaatt atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt
       61 caagatagta gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga
      121 gtttgaaaga gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa
      181 agagtctcca aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa
      241 aaaga
//
LOCUS       DAXWLG010000432          244 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.446, whole genome shotgun sequence.
ACCESSION   DAXWLG010000432 DAXWLG010000000
VERSION     DAXWLG010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.446"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>244
                     /locus_tag="ACGE4T_002954"
     CDS             <1..>244
                     /locus_tag="ACGE4T_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388141.1"
                     /translation="VHFKKTVTSDSKLRLTWTNNSNEESDIQLPIIIYNHSTVTLNGK
                     KLTPNEIKTTQIGAAIVTSSPGKNTLVIGYKPFVLFK"
BASE COUNT           93 a           34 c           33 g           84 t
ORIGIN      
        1 tgtacatttt aaaaagactg taactagtga ttctaaactt cgtttaactt ggactaataa
       61 ttcaaatgag gaatccgata ttcaattgcc tataataatt tataatcata gtacagtaac
      121 tttgaatggt aaaaaattaa ctccaaacga aattaaaact actcaaattg gagctgctat
      181 tgttacatcg agtccaggaa aaaatacttt agttatcggt tataagccat ttgttttatt
      241 taaa
//
LOCUS       DAXWLG010000433          240 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.447, whole genome shotgun sequence.
ACCESSION   DAXWLG010000433 DAXWLG010000000
VERSION     DAXWLG010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.447"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            complement(<1..>240)
                     /locus_tag="ACGE4T_002955"
     CDS             complement(<1..>240)
                     /locus_tag="ACGE4T_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8388142.1"
                     /translation="IGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTG
                     FNVSNIHVPVRESDMIFSVIGENFGFIGSCLVIFL"
BASE COUNT           90 a           45 c           33 g           72 t
ORIGIN      
        1 ataggaatat tacaagacaa cttcctataa agccaaaatt ttctccaatt acagaaaata
       61 tcatatcgga ttctctaaca ggaacgtgaa tgtttgatac attaaaacca gtacctaaaa
      121 gtccgccaga gccaatagca attaaactat ttgctaactg atagctagat ccatttggat
      181 ctaaaaatgg attcaaccat gagtctattc ttttaaactg atagtcgtga aatcctattt
//
LOCUS       DAXWLG010000434          240 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.448, whole genome shotgun sequence.
ACCESSION   DAXWLG010000434 DAXWLG010000000
VERSION     DAXWLG010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.448"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT          103 a           22 c           31 g           84 t
ORIGIN      
        1 gcgttataat aaagaacagt attatttaaa atgacagaaa acattactat taaagaaata
       61 aaaaacataa aaaatcttaa gaaatcggtt tcatttagct tttgagataa aataatagta
      121 cagttttata cgctgtttca tatgtttaga aaatttgaat tagatttgaa aactctaatt
      181 tttaaatgat aaaaaaagcg agaatgctgg ttttactgta tattaacctt agataacttt
//
LOCUS       DAXWLG010000435          238 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.449, whole genome shotgun sequence.
ACCESSION   DAXWLG010000435 DAXWLG010000000
VERSION     DAXWLG010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.449"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>238
                     /locus_tag="ACGE4T_002956"
     CDS             <1..>238
                     /locus_tag="ACGE4T_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8388143.1"
                     /translation="KEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEF
                     VYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYE"
BASE COUNT           76 a           40 c           45 g           77 t
ORIGIN      
        1 aaaagagttt gataaccaag ctattgatga attattaaca acttttgaca tcaatcgatc
       61 attgagtcat aaaggctgtc cttttgataa tgccgtagct gaatcaactt ataagtcgtt
      121 gaaagtagaa tttgtctatc aatacacatt tgaaacctta caacaattgg atttggagtt
      181 atttgactat gtcaattggt ggaaccacct tcggttgcac ggtacacttg gctacgag
//
LOCUS       DAXWLG010000436          236 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.450, whole genome shotgun sequence.
ACCESSION   DAXWLG010000436 DAXWLG010000000
VERSION     DAXWLG010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.450"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>236
                     /locus_tag="ACGE4T_002957"
     CDS             <1..>236
                     /locus_tag="ACGE4T_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010709177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8388144.1"
                     /translation="VYLIYPTLGMKNGLIVTCSYLILGIIGLPIFASGGGYAYIFKPT
                     FGFLLAFVTLPIIQSFISNKFNNAIYSSFKKNAI"
BASE COUNT           70 a           41 c           33 g           92 t
ORIGIN      
        1 tgtttatctc atttatccta cattaggtat gaaaaacggt ctgatcgtaa cctgtagcta
       61 cttaattcta ggaataatag gtcttccgat atttgcaagc ggtggtggat atgcatatat
      121 tttcaaacca acttttggtt tcttgctagc ctttgtgact ttacctatta tacaatcgtt
      181 catctcaaat aaatttaata atgctatcta ctcttctttt aaaaaaaatg ctatta
//
LOCUS       DAXWLG010000437          233 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.451, whole genome shotgun sequence.
ACCESSION   DAXWLG010000437 DAXWLG010000000
VERSION     DAXWLG010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.451"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>233
                     /locus_tag="ACGE4T_002958"
     CDS             <1..>233
                     /locus_tag="ACGE4T_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFF8388145.1"
                     /translation="IKIVLSASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFN
                     YQDKVQTSTELLMKIEKEKLLYITKTSPYNKTD"
BASE COUNT           93 a           34 c           33 g           73 t
ORIGIN      
        1 tataaaaata gtcttatcag ctagtatccc aatattaatt gatcaggctt ccgaacacag
       61 gtctttacta ttgattgttt caattgcttc agcaataatt acaattatac aaagtgggat
      121 gtctgcattt aattatcaag ataaagtaca gacttcgaca gagttattaa tgaaaattga
      181 aaaagaaaag ttgttatata taacgaaaac atccccatat aataaaacag atg
//
LOCUS       DAXWLG010000438          228 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.452, whole genome shotgun sequence.
ACCESSION   DAXWLG010000438 DAXWLG010000000
VERSION     DAXWLG010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.452"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..>228
                     /locus_tag="ACGE4T_002959"
     CDS             <1..>228
                     /locus_tag="ACGE4T_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8388146.1"
                     /translation="LKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSI
                     SNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           82 a           42 c           41 g           63 t
ORIGIN      
        1 cttgaaacca gaaggccgta aggcagttga tattgaaaat tcgattaatg aatggcttca
       61 atccgtaccc aaaaatcttt tcaaatcaat tacctttgat tgtggaaaag aattctctaa
      121 ttggaaaagt attagtaata cgaatgatat tgatatttat ttcgcagacc caggaacgcc
      181 ttcccaacgg ggattaaatg aacattcaaa cggactcctc cgaaaaga
//
LOCUS       DAXWLG010000439          223 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.denovo.453, whole genome shotgun sequence.
ACCESSION   DAXWLG010000439 DAXWLG010000000
VERSION     DAXWLG010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.denovo.453"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
BASE COUNT           99 a           33 c           34 g           57 t
ORIGIN      
        1 atatatattt cagtatcctt cttgttttca tataaccaaa ggcgctatga tttccatatg
       61 aaaaaaataa agtcaggtga aaaaaatgaa acttgaggat aaaaaagtca ttttgaagaa
      121 aatcaaacga ctaccagacc aaatagatgg ctatacgatc tgttctttca aaaaagacag
      181 gagtataaaa gcttatacgc aaaacacaga aaaaggaata atc
//
LOCUS       DAXWLG010000440        11890 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.073, whole genome shotgun sequence.
ACCESSION   DAXWLG010000440 DAXWLG010000000
VERSION     DAXWLG010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.073"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..1518
                     /locus_tag="ACGE4T_002960"
     CDS             <1..1518
                     /locus_tag="ACGE4T_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFF8388147.1"
                     /translation="QQCIKQVLEPICEAKFSENSYGFRPNRSVENAIKATYNRLQISQ
                     LHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPE
                     KGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMK
                     KTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKR
                     YSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRL
                     YNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEAT
                     RYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLM
                     YQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDD
                     YKNLILVKEAVHRLIHAKTEETIQKYIALLKRNKVQLIRLNKYRVLAGNQELNLI"
     gene            1622..1846
                     /locus_tag="ACGE4T_002961"
     CDS             1622..1846
                     /locus_tag="ACGE4T_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388148.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            1963..2460
                     /locus_tag="ACGE4T_002962"
     CDS             1963..2460
                     /locus_tag="ACGE4T_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFF8388149.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            2547..2939
                     /locus_tag="ACGE4T_002963"
     CDS             2547..2939
                     /locus_tag="ACGE4T_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8388150.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            2923..5370
                     /locus_tag="ACGE4T_002964"
     CDS             2923..5370
                     /locus_tag="ACGE4T_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8388151.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            5373..7556
                     /locus_tag="ACGE4T_002965"
     CDS             5373..7556
                     /locus_tag="ACGE4T_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8388152.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            7553..8554
                     /locus_tag="ACGE4T_002966"
     CDS             7553..8554
                     /locus_tag="ACGE4T_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFF8388153.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            8569..9483
                     /locus_tag="ACGE4T_002967"
     CDS             8569..9483
                     /locus_tag="ACGE4T_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8388154.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            9797..9844
                     /locus_tag="ACGE4T_002968"
                     /pseudo
     CDS             9797..9844
                     /locus_tag="ACGE4T_002968"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9860..11779
                     /gene="tet(M)"
                     /locus_tag="ACGE4T_002969"
     CDS             9860..11779
                     /gene="tet(M)"
                     /locus_tag="ACGE4T_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8388155.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         3913 a         2137 c         2480 g         3360 t
ORIGIN      
        1 caacaatgta taaaacaagt attagaacca atctgcgaag ctaaatttag tgaaaacagc
       61 tatggtttta gacctaatag gtcagttgaa aatgcaataa aagcaaccta taaccgccta
      121 caaatcagcc agctacatta tgtgattgaa tttgatatta agggtttctt tgacaacgtg
      181 aaccattcca agctgataaa acagatatgg gctatgggaa tacgggacaa gcacttgata
      241 ttcattttaa aaaggatatt aaaagctcca atcaaaatga ctaacggtac aataacatat
      301 ccagagaaag gaactccgca agggggaata atatccccac tactagcaaa cattgttctt
      361 aatgagttag accattgggt agaaagccaa tggcaggaaa atcctgttac aaaaaactat
      421 gtggttcata tcaataaaag cggtagccca tgtaaaagca acgcttacaa agaaatgaag
      481 aaaacaaagt taaaggaaat gtatatggtt cgttatgcag atgattttag agttttctgc
      541 cgctacaagg aaagtgctga aaaagccaaa atagcaatca ctcaatggat tgaacaacga
      601 ttaaaattgg aagtatcaca agaaaaaaca agaattgtaa atgttagaaa aagatactcc
      661 gactttcttg gtttcaagat taaaatgata ccgagaagaa agaagttagt tgttaaatca
      721 catataagtg ataaacaatt caaaaaccaa aaagacaagc tggtatcaca ggcaaagaaa
      781 attgcttctc cctcaaaagg gaaaacggaa ttagaagaaa caagacttta taattcaatg
      841 gtattaggta tgcaaaatta ttatcagatt gcaacgaatg taaatctgga ttgtatgaaa
      901 ctgaatagag ctgtaatggc tatcttcact aacagattag gtacaaacaa aaggggcaga
      961 ttgagaaaaa caggcagaca acttacgaca aaggaggcaa caagatatgg taaatctaaa
     1021 atgcttcggt atgttgctgg cgagggcgaa cctattttcc ctgttggata tatccaacac
     1081 agaaaaccaa tggcaaaggt gtatttagcg aattgctaca ctctagaggg aagaaaattt
     1141 atccacacca atctaagtgt tgataaatta ctgatgtacc agttaatgaa attatctttg
     1201 gaaaatcgca caatagaata cgcagacaat cgaatttcgc tattttctgc tcaacatgga
     1261 aaatgtgcta taactctgga agaatttcaa caagctaatg aaatacattg tcatcatatt
     1321 gtaccaactg gagctggtgg aaatgacgat tataaaaatc ttattttggt aaaagaggcg
     1381 gtacaccgtc taatacacgc taaaacagaa gaaacaatac aaaaatatat agctctattg
     1441 aagcgaaata aagtccagct cataagacta aataaatata gagtattagc tggcaatcaa
     1501 gaattaaact tgatataggt ctactacata aatacttttc aatataaaca cgatggaacg
     1561 cggagtgcgg tgaaagtcgc acgctccgtg tgaatggggg gaaaagtttg agatatttat
     1621 aatatcagga acttacctat ccatatataa ctggtttcta tcaaacgctc aatcactggt
     1681 gcttttagca atcgttgtga ttggcttgta tcttggcttc aagcgtgagt ttagcaaact
     1741 gattggcttt ttaattattg cgattattgc ggttggctta gtcttcaacg ctgctggagt
     1801 aaaagacatt ttactagagc tattcaatcg cattattggt gcttaaataa aaccgttctt
     1861 ttgtggaata taagtggttt tcttatgttc cgcaaaggaa tggtacacca aacgaagtgc
     1921 ggtagggatt tttgaatctc tacaaagaaa ggacgtgaat atatggacga tatgcaagtc
     1981 tatattgcga atttaggcaa atacaatgag ggcgaattgg tcggtgcgtg gtttaccttt
     2041 cccattgact ttgaggaagt caaagagaaa atcggcttga atgatgaata tgaggaatac
     2101 gccattcatg attacgagtt accctttacg gttgacgaat acacttctat tagcgaactc
     2161 aatcgcctat gggaaatggt atcggagttg ccagaagaac tacaatcgga gctatctgct
     2221 ctgctcactc atttttcaag tattgaagaa ctaagcgaac atcaagaaga tattatcatt
     2281 cattccgatt gtgatgatat gtctgacgtg gcacgctact acattgaaga aacgggtgct
     2341 ttaggcgaag tgccagccag tcttcaaaac tatattgatt atgaatccta tggtcgggat
     2401 ttagaccttt cgggaacgtt tatctcaacc aatcacggga tttttgaaat cgtctattaa
     2461 atctgtcggt acattcttac tggcagattt tcttgtttac ggggtagtca aaagactatc
     2521 cccattttta tgaaaggatt gattacatga agaaaatacg aagctatacc agtatctggt
     2581 ctgttgagaa agtgttgtac tccatcaatg actttagact tccgtttccc ataaccttta
     2641 cgcaaatgac atggtttgtc gtgtcactct ttgcagttat gatacttggc aacttgcccc
     2701 ctctttctat gatagaggga gcatttctca aatactttgg gattcctgtg gctttcacat
     2761 ggtttatgtc tacaaaaacc tttgatggta aaaagcctta tggatttttg aagtctgtca
     2821 ttgcttatgc actgcgacga aagctgacct atgcaggaaa aaaagtaacg cttggcagaa
     2881 accagccaca agaagccatt acagcagtta ggagtgaatt ttatggcata tccaattaaa
     2941 tatattgaaa acaatctggt ctggaataaa gatggggaat gttacgctta ctatgagctt
     3001 gttccctaca attactcatt tctaagtccg gaacagaaaa tacaagtaca tgattctttc
     3061 agacagctta tcgcacaaaa tcgtgatggc aagattcatg ctttacaaat cagtacagaa
     3121 tccagcatac tttctgcaca agaacgttcc aaaaatgaag tcactggaaa gctcaaaacg
     3181 gttgcctatg acaaaatcga ccaacagaca gacgctttaa tatccatgat tggcgaaaat
     3241 caagtggact accgtttctt tatcggtttt aagttgcttc tcaacgatca ggagttttct
     3301 atgaaaagtc ttaccgttga agcaaaaaat gcttttactg attttgtcta tgatgtgaac
     3361 cataagctga tgggcgattt tgttagtatg agtaatgatg aaatcctgcg ttttcagaag
     3421 atggaaaagc ttttagaaaa taaaatctcc cgtcgtttca aaatccgaag attagataag
     3481 gatgacttcg gctatctgat tgaacacctt tacggacaga caggcactgc ctatgaagag
     3541 tatgagtatc ctctatcaaa gaaaaagctg gaacatgaaa cactgattaa aacctatgac
     3601 ctcattaagc ctactcgctg tctggtggaa gaaaaacagc gatatttgaa aatccagcag
     3661 gaagacgaaa ccgcctatgt agcttacttt accatcaaca gtattgtcgg cgaactggac
     3721 ttcccgtcct ctgaaatctt ctactaccag caacagcaat ttacctttcc gattgatacg
     3781 tcaatgaatg tggaaattgt agcgaatcgt aaagccctat ctactgtccg caataaaaag
     3841 aaagaactga aagacttgga taaccacgct tggcaaagtg ataatgaaac cagctctaat
     3901 gtggcggaag ctctggaaag tgtgaacgag ctggaaacca atttagacca aagcaaggaa
     3961 tctatgtata agctgtccta tgttgtaagg gtatcagcaa atgaccttga cgaacttaaa
     4021 cgtcgttgta atgaagtgaa agatttctat gatgatttga gcgtgaaact ggtacgacct
     4081 tttggggata tgctgggctt acatgaagaa tttttacctg ccagcaaaag atatatgaat
     4141 gactatattc aatacgtgac ctctgatttc ctcgctggtt taggttttgg tgctactcaa
     4201 atgctgggtg aaaatgaggg gatttatgtt ggctacagct tagatactgg acgcaatgtc
     4261 tatctgaaac ctgctcttgc cagtcaaggg gttaagggtt cagtaaccaa tgcgttagcg
     4321 tctgcctttg tcggttcgct gggtggtggt aaatcctttg cgaataacct tatcgtctat
     4381 tatgcagtgc tttatggggc acaagcagtg attgtagacc caaaagcaga acgtggcaga
     4441 tggaaagaaa ccttgccaga gatttcccat gaaatcaata tcgtcaatct gacttctgat
     4501 gagaaaaaca aaggcttact tgacccttat gtaattatga aaaataccaa agattctgaa
     4561 tcactggcta ttgatatttt gacattcctt acggggattt cctctcgtga tggggaacgc
     4621 ttcccaatcc ttagaaaagc cattcgtgca gtaaccaata gtgaagtgcg agggttgatg
     4681 aaagtgattg aagaattacg ggttgagaat acgccactaa gtaccagcat agccgaccat
     4741 atcgaaagtt ttacagacta tgactttgcc catctgcttt ttagtgatgg ttatgtagag
     4801 cagtctatca gccttgaaaa acaactgaac attatacagg ttgccgactt ggtacttcct
     4861 gacaaggaaa cttcctttga ggaatatacc acaatggagt tactatcggt agcaatgctg
     4921 attgtcatta gtacctttgc gttggacttt atccatacag accgaagtat tttcaagatt
     4981 gtagacttag acgaagcatg gagcttctta caggtggcac aagggaaaac actatctatg
     5041 aagctggttc gtgctggtcg tgctatgaac gctggggtat atttcgtgac ccaaaataca
     5101 gatgacctct tagatgaaaa actgaaaaat aacctcggct taaaatttgc ctttcgttcc
     5161 actgacctta acgagattaa aaaaaccctt gccttttttg gtgtagaccc agaggacgaa
     5221 aacaatcaga agcgattgcg tgatttggaa aacgggcaat gccttatcag tgatttatat
     5281 ggtcgtgtcg gtgtgataca gttccaccct gtatttgaag aactgctcca tgcctttgat
     5341 accagaccac ctgtgcgaaa agaggtgtaa atgtgaaacc atcaatatta aacagaataa
     5401 aatcaaactg gacgctgaaa cgtctaggta aagtggcaat gacagtggct ttcacacttg
     5461 tgattgccat ttttctttta gccatgctgg gaacggtggt tcaagctgcg ggcttggtag
     5521 atgatacggt caatgtggca aatgaataca gccgataccc acttgaaaac tatcaactgg
     5581 atttttatgt ggataatagc tggggctggc taccgtggaa ctggtcggac ggaattggaa
     5641 aacaagtcat gtatggacta tatgccatta ccaattttat ttggacaatc agtctttatg
     5701 tttccaatgc gacgggttac ttagtacagg aagcctattc cttagacttc atttccgcta
     5761 cagcagattc cattggcaag aatatgcaga cccttgcagg ggttacgcct agtggactat
     5821 caacagaggg tttctatgtt ggattccttt tactcttgat tttggttctt ggggtttatg
     5881 ttgcctatac aggactgata aagagagaaa ccacaaaggc aattcatgcc ctcatgaatt
     5941 ttgtgctggt ttttatccta tcggcttcct ttattgccta cgctcccgac tacattaaaa
     6001 aaatcaatga cttttcatca gacatcagca acgctagttt gtcactgggt acaaaaattg
     6061 tcatgcccca ttcagatagt caaggcaagg acagtgtgga cttgatacgt gacagcctat
     6121 tttccataca ggttcagcaa ccgtggctac tgcttcaata taacagttca gacattgaaa
     6181 gtattggtat tgaccgtgtg gaaagcctgc tctcaaccag tccagattcc aacaatgggg
     6241 aagacagaga aaaaattgtt gcggaagaaa ttgaagatag aagcaatacc aatatgacga
     6301 ttacaaagac gattaaccgt ttaggtacag tcttcttcct atttgtcttc aatattggga
     6361 tttccatatt tgtattccta ttaacaggaa tcatgatttt ctcgcaggta ctttttatca
     6421 tctatgctat gtttctgcct gtgagcttta ttttaagcat gattccatca tttgatggta
     6481 tgtcaaaacg agccataaca aagctcttta ataccatttt gacacgagct ggaatcacat
     6541 tgattattac gacagcattt agtatttcaa ccatgctcta taccttatcg gctggttatc
     6601 cgttcttttt gattgctttt ctacagattg tgacctttgc aggaatctac tttaagctgg
     6661 gcgatttaat gagtatgttt tctctacaga gtaacgattc tcaaagtgtg ggaagtcgta
     6721 tgatgagaaa acctcgtatg cttatgcacg ctcacatgca ccgtctacag cggaaacttg
     6781 gacgctccgt gactgcttta ggggctgggt ctgccattgc cagtgctacg ggtaaaaaag
     6841 gacagtcggg ttcgggaagt tctgcgagta cacaagcaga tcactctcga ccaaacgggc
     6901 aggaaaaatc aacactagga aagcgtatcg gtcaaaccat cggtacagta gctgatacca
     6961 aagacagaat ggtagatact gctggtaatt tgaaagaaca ggttaaggat ttgccgacca
     7021 atgcaagata tgcagtatat caaggaaaat ctaaagtgac ggataatgtc catgatttaa
     7081 ccagaagtat ttctcaaacc aaagcggata aagccagtgg acgcaagaaa cagcaggaac
     7141 aaagacggaa aaccattgcg gagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac
     7201 agccagcttc ttccgttcat gaaagaccag ccactaagca agaacaatct catgatggac
     7261 agacttcaag gcaaacaact gtacaggctt cctataggga atctcaacaa gccaaacaag
     7321 agcgtccaac agttaagtcc gattcttcaa gtttaaaaac ggaacgtcaa agtaatacag
     7381 ttcaagaaag aacggttcaa aagccagtaa cttcaactgc accaacagat agagcttcac
     7441 aacgttcaat cacaaaagaa cgtcagtcta ctgttcaaag agtaccacta caaaatacaa
     7501 aaagtagacc accaatcaaa accgccacca ttaagaaagg cagtaagaaa ccatgaagct
     7561 gaaaacttta gtgattggtg gttcgggatt attcttgatg gtcttctcac tgcttctgtt
     7621 tgtcgccatt ttattttcag atgaacaaga tggcggtttt tccaacattc actatggcgg
     7681 tgtggatgtt tccgcagaag tgctggctca taagcctatg gtagaaaaag ttgccaaaga
     7741 atatggaatt gaagaatacg tcaatatact tcttgcgatt atacaggtgg aatcgggtgg
     7801 tacagcagaa gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag
     7861 tacagaagaa tccattaagc aaggtgtgaa gtatttcagt gagttattag ccagtagcga
     7921 aaggcttggt gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt
     7981 aggatatgtg gctaatcgtg ggaataaata tacctttgaa ctggctcaaa gtttctcaaa
     8041 agagtattcg ggtgacgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg
     8101 gggctggcga tacaattacg ggaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt
     8161 cacaacagag tttgatgatg atacagtaca agccatcatg gacgaagctc tgaaatttga
     8221 aggctggaag tatgtctatg gcggagcttc cccgactacc tcttttgatt gtagcggact
     8281 gacacaatgg acgtatggaa aagctggaat caacttacca cgaacagcac aacagcaata
     8341 tgatgtaacc cggcatatcc cattattgga agcaaaagcc ggcgatttgg ttttctttca
     8401 ttctacctat aacgctggct cttatattac tcatatcggg atttatttag gcgataaccg
     8461 tatgtttcat gcaggcgacc ctatcggtta tgccgactta acaagctctt actggcaaca
     8521 gcatttagtg ggagcaggac gaatcaaaca ataagaaagg acgattcaat gatgaaattc
     8581 ggaaaaaatc agaatacaga aaaacagaca ccaaaggaaa agaaacctcg tgtctataag
     8641 gtcaatcctc gtaaaaaggt tgtgattgtc ctatgggtac ttttaggact tagtttcagc
     8701 tttgcgatat tcaagcactt tacagccatt gatacccata ccattcatga aaccaccatc
     8761 atagaaaagg aatatgtcga tactcatcat gtggaaaatt ttgtagagaa ctttgcgaaa
     8821 gtctactatt catgggaact aaacgacaag tccattgata atcgcataga aaacctgaaa
     8881 gcatatctga cagaggaact tcaagctctg aatgtcgata cagttcgtaa agatattcct
     8941 gtatcgtctt ctgtaaaagg atttcagata tggacggtag aagcagatgg cgacaatcaa
     9001 tttgatgtaa cctacagtgt agaccaactc attacagagg gggaaaatac aaagaccgtc
     9061 cactctgctt atatagtgag tgtctatgta gacagtactg gaaatatggt cctcattaag
     9121 aatccgacca ttactagcat accaaagaaa tcagactata aaccaaaagc tattgaaagt
     9181 gagggtacgg ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc
     9241 aagctctatc ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta
     9301 aaaccaatcg gaaaagagta catctttcaa gaactggtaa atcctattta caatcgtaag
     9361 gataatcaag tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg
     9421 caggtatctc aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa
     9481 taacaaatat tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa
     9541 attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag
     9601 gagttaataa atatgcggca aggtattctt aaataaactg tcaatttgat agtgggaaca
     9661 aataattgga tgtccttttt taggagggct tagttttttg cacccagttt aagaatacct
     9721 ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat
     9781 gcataaaaat cccagtgata agagtattta tcactgggat ttttatgccc ttttgggctt
     9841 ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc
     9901 gggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg
     9961 aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat
    10021 tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga
    10081 cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg
    10141 ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca
    10201 tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg
    10261 aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat
    10321 caaacagaag gtagaactgt atcctaatat gtgtgtgacg aactttaccg aatctgaaca
    10381 atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc
    10441 attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt gctccttgta
    10501 ccctgtttat catggaagcg caaaaagcaa catagggatt gagcagctta tagaagtgat
    10561 aacgaataaa ttttattcat caacatacag aaagaagtct gaactttgcg gaaatgtctt
    10621 caaaattgaa tattcggaag aaagacaacg tcttgcatat gtacgccttt atggcggaat
    10681 cctgcatttg cgggattcgg ttagaatatc ggaaaaggaa aaaataaaaa ttacagaaat
    10741 gtatacttca ataaatggtg aattatgtaa aattgataag gcttattccg gggaaattgt
    10801 tattttgcaa aatgagtttt tgaagctaaa tagtgttctt ggagatacaa agctattgcc
    10861 acagagagag agaattgaaa atccgctccc tctgctgcaa acaactgttg aaccgagcaa
    10921 acctcaacaa agggaaatgt tacttgatgc acttttagaa atctccgaca gtgacccgct
    10981 tctacaatat tatgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt
    11041 acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa
    11101 agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat
    11161 cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt
    11221 gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca
    11281 aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg gttggaatgt
    11341 gacggattgt aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc
    11401 agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga
    11461 attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc
    11521 atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga
    11581 ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac
    11641 tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac
    11701 cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat
    11761 gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat
    11821 aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggaa ttaattatga
    11881 agtgtaatga
//
LOCUS       DAXWLG010000441         6590 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.135, whole genome shotgun sequence.
ACCESSION   DAXWLG010000441 DAXWLG010000000
VERSION     DAXWLG010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.135"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            46..1314
                     /locus_tag="ACGE4T_002970"
     CDS             46..1314
                     /locus_tag="ACGE4T_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8388156.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1801..2265
                     /locus_tag="ACGE4T_002971"
     CDS             1801..2265
                     /locus_tag="ACGE4T_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8388157.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2249..2878
                     /locus_tag="ACGE4T_002972"
     CDS             2249..2878
                     /locus_tag="ACGE4T_002972"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8388158.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            3232..4710
                     /gene="msr(C)"
                     /locus_tag="ACGE4T_002973"
     CDS             3232..4710
                     /gene="msr(C)"
                     /locus_tag="ACGE4T_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8388159.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4757..5203
                     /locus_tag="ACGE4T_002974"
     CDS             4757..5203
                     /locus_tag="ACGE4T_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFF8388160.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5402..5917)
                     /locus_tag="ACGE4T_002975"
     CDS             complement(5402..5917)
                     /locus_tag="ACGE4T_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8388161.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            6034..6590
                     /locus_tag="ACGE4T_002976"
                     /pseudo
     CDS             6034..6590
                     /locus_tag="ACGE4T_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         2125 a         1142 c         1363 g         1960 t
ORIGIN      
        1 acactgttat ttaggaaaag tttcataatt caaaggagaa aaaagatgaa agcaaagaat
       61 ttattattta gcgttattct tttaggaaat ctagcagttg ccaacacaac agtgctagct
      121 gaagaatcga cagaaactgt cgcaactcag acatcgagtt cttcagaaac aggaattgaa
      181 aacgatacag agagtacttt gcagtcagct actgtctcag aaaatacaat ttcaaccgaa
      241 gaaccaacaa ctgaaacaac agaacaaaca caaaccgaag acagtacatc agcagaagct
      301 cagaacaatc cagagaatgc agaagaaatc acgaaccaat tgacagatgg gcaaacattg
      361 acacataccc atcaagcaga tttgtctgaa ttcgaaaact atactggcta tgctttttat
      421 gctcgctcat ctgctaattc tcaacaagcg ttcattgata gtattgcctc aactgcacaa
      481 agtttggcaa gtgccaatga cttatatgca tcagtcatga ttgctcaagc catcgttgaa
      541 agtggctggg ggaacagtac tttggcttct gcccctaatt acaatctatt tgggattaaa
      601 ggaagctata atggccaatc tgttacgatg cctacttcgg aatatgtgaa tgggcaatgg
      661 attacggtga atgccgcttt tagaaaatat ccttcgtaca aagaatcatt gcaagataac
      721 gtaacggtat tgaagacgac ttcttttcag ccaggcgttt actactattc cggtgcatgg
      781 aaaagcaata cgaactctta caaagatgct acggcttggc taacaggtcg ttatgccact
      841 gcgcctaatt atggttctac attgaacaat gtaatcgaaa cctataatct gacacagtat
      901 gatacagcac caacttcaac ttctccgatg tatcgtctgt ataaccgtca tacaggagaa
      961 catttatata cattgaatgc cggagaaaaa aattatctgc caactgtagg ctgggaatat
     1021 gaagggattg cttggcaagc accaaacagc ggacaacctg tctaccgttt atacaatcct
     1081 tacagtggag accatcatta cacaatggca caatctgaga tcaactcttt aacaaaaatc
     1141 ggttggcgtt atgaaggtct aagtttttat tctggtggtt ccaagccaat ttatcgacta
     1201 tttaatccaa atgaaaaaac tggtacccat cactatacct tgagtgctaa ggaaagagat
     1261 tggctaacac cgatgggctg gagatatgaa ggaataggat tttatggtta ttaaaaagag
     1321 gtagtgaaaa agctgccctg catcaagtag taagaacagc caaacaaaaa cagattttca
     1381 tgttttttgt ttggttgaac ttattaccgc agatgcaagt ttttgaacac cgtttactca
     1441 aaaaaggttg tgacaacatc ttgtcataac ttttttatta ctgtttaagc atactgatcc
     1501 aatttctaat attcgacggg attcttgcaa aaggcgattt cggaaatttt tggcggtgta
     1561 aaattttcta ctgcagaaga gctatcttct atagtttgat aagaatcaat aatcttggtt
     1621 ttagcgttgt gctttcttta tcattttttc cgttcattct acaaaaaatt tcttttctct
     1681 atacttttct attgagagcg ttttttttat tacacagctg attctttgac ttttttcttt
     1741 ttttattcta ttattgacaa gtcaatcgtt gacgagtcaa ttattagaaa ggaggaaatt
     1801 atgtctttag aagagattag tcacctttta tatcaaatca aattagccga tcaaaaaatc
     1861 gtccagatct ttgaagaaaa tatcggtata agtcttacta gatacgagat catgatgaga
     1921 ctgaaagaag aaaatccatt gctacaaagc agtttgcaag aaaagttgca gattgatcaa
     1981 ggagcagtta ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc gcgtcagaga
     2041 aatcctcaga acaaccggga agtgtatgta gcgctaacag aaaaagccca gaagaaaatc
     2101 gagcattgtg aaatggacca taaagataca ggttccgtgc tatccccggc tttcagcgat
     2161 gtagaaatga aacaatttct aacactttta gataaattga atacccaact ttgcaatata
     2221 acaacagaaa cgaaagaggt taataataat ggaacaaaaa aatgattttt caacaattat
     2281 gactggtaga agatccgttc gcgtatatga tgaaacatac aaaatcccac acgaagaaat
     2341 gctggaaatg atcaaagaag cgacgactgc accttcttct gtcaatatgc aaccatggcg
     2401 ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga tccgtttcaa
     2461 tacgagacaa aatgatactt cttctgcaat ggtcatgatt tttggcgata tgcaatgtta
     2521 cgaatatggc gaagaaatct ataaccaagc ttacgaatca gggaagatgc caaaagaagt
     2581 cagagatcaa caattagctg cgatcattcc ttattacaag agtttttcaa gagaagaaat
     2641 gaacgatgta gtcaaagtcg attctagctt agcggctatg caattcatgt tagttgcgcg
     2701 tgatcatggc tatgaaacaa atccaattgg tggattcgaa gcagatcagt tggcagaggc
     2761 atttggtttg gataaagacc gttatgttcc agttattatt ttatcaatag gaaaagcggt
     2821 agaagaaggt tacgagtctg ttcgtttagc accagaaaag atcactacgt tcgaataaaa
     2881 ggttttcagt tactattcag tagctattgc tttttttgaa tcgtgtgtgg taacctaata
     2941 aaaaaggagg tcggaaaaat gactgcatcg atgaaattac gtttcgaact tttgaataac
     3001 aactaaataa attcaaaaga catgtataaa caacaatttc atggatggag tatgtccatt
     3061 ttttcgaacg acagtagaat aggtgaaagc tatgacagat ttggtgattt agtcatagtt
     3121 atttcgttca gagtaggaaa agatgtgatg cagttttttt gcaaacagat ttttctgctc
     3181 ttttttagta tcgataattg ttgttttaca tgaaatagga gtgtaaaaca tatggaaaat
     3241 ttagcagtaa atataacaaa tctacaagtc agctttggca accagctaga actatctatt
     3301 gattctcttc gtgtctatca gcaagatcgt atagggatca taggagaaaa tggtgtcggt
     3361 aaatcgactt tgctcaaact aatagccggt gaactttttc ccgatcatgg gaaaatccaa
     3421 acagagatca ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc aaaggaccta
     3481 aatttggaat tagccagtca tttccagtta agactggaag aaacttcaga gcggaaatgg
     3541 agcggagggg aagaacgaaa gatcgagttg atacgtcttc tttcttctta tgaacaaggg
     3601 atgcttctag acgagccaac aacccatcta gatagaaaaa gtattgatcg actgattgaa
     3661 gagcttcgtt attattatgg tacgctggtt tttgttagtc atgatcgcta ttttctagat
     3721 gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt ttcggggaat
     3781 tatagtgcct atctcactca aaaggaattg gagaaaaaga ctcagctacg agaagcagag
     3841 tcgatcatga aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa aaaacaagcg
     3901 gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagacc ggatcggttg
     3961 tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc gaaaacatta
     4021 gagagaagac tccaaaaaat aggagaaaca accaagccgc agcagatgaa acaaatccgt
     4081 tttccagtac caaaatctct tgaactccac agccgctatc ccatcatggg acaaaatgtc
     4141 caattggaaa gaagtgggag aacattactg gtaaatggtg attttcagtt ttctttaggt
     4201 aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt agaacatatc
     4261 cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa gctttcaagt atatcagcaa
     4321 aagggttatc aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag acaaacagag
     4381 ttttcagaat cgcttgtccg tagtttgctg aatcacttag ggtttgctca ggaaactttg
     4441 acgaaaccgt tatgtacatt aagtggggga gaagcgaccc gtctgacgat tgctttgctt
     4501 tttactaagc caagtaatgt gttgctgtta gatgaaccga ctaattttat tgatatggca
     4561 acgatcgaag ctttagagaa gctaatgcaa atatatccgg gaacgatttt gtttacttct
     4621 catgattctt actttgtcga gcgtacggct gatgaagttt atgaaataaa agggcagaaa
     4681 ataaaaaaag tacttacgag aaatttttaa atgtgctacg atgattttcg tgacaaattt
     4741 aaaggagtgg tacagattgc ctacaaataa aaaagaagga ataattttta caacaatgat
     4801 gtgttttttg atggtcttgg ggatgagcgc gtataattta tggctgcatc aagatcttca
     4861 tttatcaagt ttgttgattg gtctagtgcc tggattcgtg gtggctttca ttttggatgt
     4921 cttcatcatc ggtgtgctgg cgaaaaaaat tgcctttaaa ctaccagtaa atccagaaag
     4981 caaggtccaa atgatcttaa ctatttcatg cttgatggtt ttaggaatgg tgacatgtat
     5041 gtctttattc ggtgtgatca tggaacaagg aatacctgat caattattct ctgcttatat
     5101 gaatgcttgg aaaatgaatg tgattgccgc tttaccgttg cagttgatca tcgttggccc
     5161 gctttcaaga agtgtgctaa aagcaattca aagtaatgaa tgatggaaca ctgttatttg
     5221 aaaaaaggcc ttggcatgct ttttgtcata gtctcttgaa tgcagttcta gaaaaatagt
     5281 gatccgaacg ataggttttg gcctgtcgtt cggatcgctt tttcttttcc aactgtattg
     5341 ctcttttatt gaagataatt tgctcttcaa tagatatttc tagaaaggtt ctgtttatcc
     5401 cttactcaaa aaactgctgc atttgttctc ttacttgctg acaccattgg agttcctgct
     5461 gacaaagcat aattctgcgc tggatcacaa cataacgtcc aaattcttgt ttcttgtcta
     5521 gcgagaggtc attcaatttt ttttgatttt tttgcatgat tttgttcagc tgttgttctc
     5581 tagtgaatag cagtccagta gctgttgttt tatcgagcgt cgaaaaagca tagatttttg
     5641 ctagaaattc atctttttgc gtaggagcgg gagtttcctc tttgatccat tcctctacaa
     5701 gagcacgtcc ttcatcagtc aagtggtaga tctttttttg agagtgctcg tctttgtcca
     5761 aaacggtcac ataaccctgc tggtctaact tggcaagcgt tgtatagatc tgactgtgat
     5821 gggcttccca aaaaattgtg atcagctgct tcaattcata cccagaacgt ggtttggata
     5881 aaagcacgca taataatata taggctaaag tattcataaa aatcctacct ctcgtcttat
     5941 tttctttatt atatcacagt aaagtttcca cgttttacat gtgcatattt acatgtaaaa
     6001 taaatcatgt atgatgctaa tagagagaaa gggatggaaa tattggagaa tcaaatagat
     6061 atacaagaac aaacaaatga agaaacaaag gggaaaagtt cagcgctagt tgtagcagct
     6121 acctgtatga tcgcttttat gggagtgggt ctagtagacc caatcttaaa aacgattgct
     6181 gcacagttaa acgctacgcc agctgaaacg accctgttat ttactagtta catgttagtc
     6241 actggtgtgg tgatgctatt tacaggtttc ttatctagtc ggatcggttt gaaaaaacat
     6301 tgatgacagg attgtttatc atcgttgtat tcgctggtct tggcggatta tctgggaata
     6361 tcggaatgct gattgggtta cgtgccggct ggggtgtcgg gaatgcttta tttgtctcta
     6421 ctgctttagc aacgatcgtc agtattttag ttggtaacac agaaaaagca atcatgatgt
     6481 atgaagcagc acttggctgc ggaatggctg ttggccctct agtcggtggg gtcttgggca
     6541 gtggttcatg gcgttatcca ttttttgggg tagcattttt gatgttttgc
//
LOCUS       DAXWLG010000442         5252 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.163, whole genome shotgun sequence.
ACCESSION   DAXWLG010000442 DAXWLG010000000
VERSION     DAXWLG010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.163"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            241..1209
                     /locus_tag="ACGE4T_002977"
     CDS             241..1209
                     /locus_tag="ACGE4T_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8388162.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE4T_002978"
     CDS             1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE4T_002978"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8388163.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2239..2847
                     /locus_tag="ACGE4T_002979"
     CDS             2239..2847
                     /locus_tag="ACGE4T_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8388164.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE4T_002980"
     CDS             3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE4T_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8388165.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE4T_002981"
     CDS             4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE4T_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8388166.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5111..>5252)
                     /locus_tag="ACGE4T_002982"
     CDS             complement(5111..>5252)
                     /locus_tag="ACGE4T_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFF8388167.1"
                     /translation="LTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILSAIR
                     VF"
BASE COUNT         1672 a          903 c         1166 g         1511 t
ORIGIN      
        1 tggtgtcatc caccaacatc aaaaagtata gagccataat tttttaggaa aatctcaagg
       61 ttatctttac tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac
      121 acggtagact taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa
      181 gattaccatt ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt
      241 atgaataaca tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat
      301 gctctttcgc ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc
      361 aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc
      421 gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc
      481 ggctgcaatc atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg
      541 gcgtactcgc cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc
      601 aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt
      661 ggcaaggtac tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg
      721 gttattgagc ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt
      781 atagaggtaa actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt
      841 catgtgccgc tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg
      901 aagcaaggag catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg
      961 gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag
     1021 gaagagtttt tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa
     1081 cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg
     1141 ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag
     1201 catgaataga ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc
     1261 ggtaaaatct gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata
     1321 cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga
     1381 aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt
     1441 taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg
     1501 caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt
     1561 aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc
     1621 gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt
     1681 ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg
     1741 tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata
     1801 tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt
     1861 gggaaacagt gccgcgttag ctgttggcga ggtggaccaa atcaggctgc agtacggaat
     1921 ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt
     1981 tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata
     2041 taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg
     2101 cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg
     2161 tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc
     2221 gttaaagggg tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt
     2281 gttcgttggg acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt
     2341 tatgaagtaa atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa
     2401 gaactggctg ctacccaagg gtacggattg cttctatggg acggttaccg tcctaatcgt
     2461 gctgtaaact gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt
     2521 tattatccca atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca
     2581 agccatagcc gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt
     2641 gtaccaatgg ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga
     2701 atatcatgca atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg
     2761 tttgaagcat atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat
     2821 agctattttg atttccccgt taaataaact tttaaccgtt gcacggacaa actatataag
     2881 ctaactcttt cggcaggaaa cccgacgtat gtaactggtt cttagggaat ttatatatag
     2941 tagatagtat tgaagatgta aggcagagcg atattgcggt cattatctgc gtgcgctgcg
     3001 gcaagatagc ctgataataa gactgatcgc atagaggggt ggtatttcac accgcccatt
     3061 gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga aaattcatct
     3121 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
     3181 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
     3241 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
     3301 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
     3361 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
     3421 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
     3481 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
     3541 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
     3601 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
     3661 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
     3721 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
     3781 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
     3841 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
     3901 aattcaatat gaactatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
     3961 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
     4021 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     4081 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     4141 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     4201 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     4261 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     4321 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     4381 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc agtatttaat
     4441 tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt cagagagatg
     4501 atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa ttttaaagaa
     4561 atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt tgaaataatt
     4621 caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa tactgttgga
     4681 ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca aaaaaaatta
     4741 gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt ttaccgtacc
     4801 catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct ttcatacaca
     4861 taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac tcgcccgtag
     4921 gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct tacctaacac tatagtaaca
     4981 aagaaagccc aattatcaat tttagtgctg aggaattggt ctctttaata aatttcctta
     5041 acgttgtaaa tccgcatttt cctgacggta cccccaaact aagatttcat agatagattt
     5101 tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc ttcattcgct aaagagtaat
     5161 aaactacttt tccatcttga cgtgattttg ccattccatg tttttttaaa aaacgtaaat
     5221 ggtgtgacgt tgtagcgaca ctagcagtaa gt
//
LOCUS       DAXWLG010000443         5153 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.164, whole genome shotgun sequence.
ACCESSION   DAXWLG010000443 DAXWLG010000000
VERSION     DAXWLG010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.164"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            138..830
                     /locus_tag="ACGE4T_002983"
     CDS             138..830
                     /locus_tag="ACGE4T_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8388168.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1157..1654
                     /gene="dfrG"
                     /locus_tag="ACGE4T_002984"
     CDS             1157..1654
                     /gene="dfrG"
                     /locus_tag="ACGE4T_002984"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8388169.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1726..3678)
                     /locus_tag="ACGE4T_002985"
     CDS             complement(1726..3678)
                     /locus_tag="ACGE4T_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFF8388170.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3685..3921)
                     /locus_tag="ACGE4T_002986"
     CDS             complement(3685..3921)
                     /locus_tag="ACGE4T_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388171.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4323..4613
                     /locus_tag="ACGE4T_002987"
     CDS             4323..4613
                     /locus_tag="ACGE4T_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8388172.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4885..>5153)
                     /locus_tag="ACGE4T_002988"
     CDS             complement(4885..>5153)
                     /locus_tag="ACGE4T_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FosX/FosE/FosI family fosfomycin resistance
                     thiol transferase"
                     /protein_id="HFF8388173.1"
                     /translation="VKDLWIAVMQNSSNKLPKTYNHIAFKIDEQDIDSFVSKIQMLGL
                     TVEPGRSRVKGEASSIYFYDYDNHLFELHTGTLQERLKSYNSTT"
BASE COUNT         1738 a          857 c          902 g         1656 t
ORIGIN      
        1 agataaaaaa ggaggattaa atgagtgttt gatagtattt atattaaaat tacatatgta
       61 atttaaaaac gcttgacatt gtgtttgaga tcacttactt ttagcatgta aaagtattgg
      121 attaaggagc tatgcaaatg aaaaagaaat ttattactgg tgggattgtc gttgcatttc
      181 tatgtctaat tggctacaca ggcacgcggt acgcaaatga aattattttg tggggtggag
      241 agacagatat tgatacaatc aatgagaatc tgacacagct tgatcataca ttgacagata
      301 aagagcaagc gattacagat cttaccgctc gactagcaca aaaagaacag acattgcaag
      361 attcaaaggc agatgtccaa gaataccaaa caaaagtaaa tgaattagaa aaccaaaaaa
      421 atcaatcggc agcagaaaaa caaaacttag aaagtcaatt agtcagcaag gatgcacaac
      481 tacaaagcaa acaaaatgaa atcaatgtca aactagatga gatcaaccaa aaaaatgatg
      541 aaatcaatca tctgaatgca gattggacag caagactaca agaagcacaa gcaaaacttt
      601 cagaattaca aaatactgta aatggtttga atcaacaaat cgcaacactg acacaagaaa
      661 aaacaaatgt agaaactcag ttaacagata cacaaaagaa attagacgaa gcaactgctg
      721 aaaactcaag cttgaaacaa tatattcaaa agttagaaaa agcaaaagat gaagtggaag
      781 atacagctga taaatctcag caaattgttg aagaacattt gcgtaaataa tctttcttaa
      841 caagtaaacc taaattagta aagaagcaac agtaatctat actaagtagt tcagttttgg
      901 agtacaaaat gacaaaatgt acttcaaaat ggactacttt tttcatttta acttacatac
      961 caaattacat acaaaatgat ttataaaatg caattgaaaa tgataattga atgaaaattt
     1021 gtgattttgt ggtattatga taatataact tatagaaaaa attgcttatt caactaacgg
     1081 gcaggataat gagaaaaggt tttttgatag aaataagaaa ttagatggta aattagttaa
     1141 aaaggctgtg ttataaatga aagtttcttt gattgctgcg atggataaga atagagtgat
     1201 tggcaaagag aatgacattc cttggaggat tcccaaggac tgggaatatg ttaaaaatac
     1261 tacaaaggga catccgataa tattaggtag gaagaacctt gaatcaatcg gaagagcctt
     1321 acctgacaga agaaatatta ttctgacgag agataagggg tttaccttta atggttgtga
     1381 aattgttcat tcaatagaag atgtttttga gttatgtaaa aacgaagaag aaatttttat
     1441 tttcggagga gaacagattt ataatttgtt tttcccttat gttgagaaaa tgtacatcac
     1501 aaaaatacat catgaattcg aaggagatac tttttttcca gaagtgaatt atgaggaatg
     1561 gaatgaggta tttgcccaaa aagggataaa gaatgataaa aatccgtata actactattt
     1621 tcatgtatat gaaagaaaaa acttattgag ttaacgggtg cgttaattga agaagaacag
     1681 taaagataaa gcacaagagc ttgggattac tccaagctct ttttgtcatt tgaaataaat
     1741 tatatctttc caatcaaatt gtttatctgt aatccctaat ctttgagcag gtgtcaaaac
     1801 ctttttatcc gctgatttaa aagggcgaca aaagttgtag taagttctta gaattgtaat
     1861 cgcaaattga gcatacttcg gattgaagtt agcgtaaata tagctcttct tatcaccacg
     1921 agctgttgtt aatggtcttt caagagatga tatataacgt cttatctgtt gaataaagct
     1981 attggtcgaa tggtcattaa catttaatag catctttgcg atttcgtttg gttccagagc
     2041 agataaatcc gttctacaat caactgaata aaatccttta tccttagtgg ctaatggatg
     2101 tttgataggg ttatcggcat atttgcgata tctttctcca tctttactaa tagcctcttc
     2161 gtggaattga tgacttgtta gtaattcagt tagataatga gatgccaaag tccgtagaaa
     2221 ctttgttttg aatcctctga tatctcccca atcgagcagt tctgcttttg cttgtccgaa
     2281 ttcttttaag cattgttttc tgcttttcgt cttgttaact tggcaaataa aatgatgtgc
     2341 atcgctcaac cttaattctt tcgtgaatac tctataaaag gcgtttctaa tagagaagtc
     2401 atcatcacta atcattcgcc attcgtcggc attaaccatt tgtttaatta accaatagtg
     2461 agcagttgta gtataaggag catcaacatt taaaccatcg acaaatctac tcctattagt
     2521 aattttccct aattcgtgaa gataagcatt cctattttcg ctatcgttag cggatggttc
     2581 ttgtggataa taagaccaat ctaacctatc atttttacga ctaaaagtat tgagatggtc
     2641 ttctttgaat tttcttgtat cttcatttaa ttcatccatt gaaatattcc aatcataggc
     2701 aacatcagaa cggaacacat atcttgataa gacctcagca gatacaacta tatacgtttg
     2761 catattaagg tcttcaaacc ccgtatattt ctttgaacct tgtcctttct tacggacgtt
     2821 atttaggtag tagtgcatct tgtccgtatt aagccacatc tcgttaaatt tctttgtttg
     2881 aaggggttgt gtctcatatc gttctaaaaa ctccaaacaa cgcctataca gccactccag
     2941 cttatgatag taggttccaa ctccaattcc taaaatatca caagtacgac tgactgaaac
     3001 ccttccaaca accatcttag cgaacattgg aagaatggtg ttcttttgtt ggtgataagt
     3061 agtggtttct tctctttttg gtagaacatt agtgaacttc ttacacgcct tacattgata
     3121 gcgttgagat ttgcccctac ttttccccct tttgtaaaat tgcttcggct cattgaatgg
     3181 ggttgattct tcttccgagc agccttcttt atgaaattga taatctggct caacatcttg
     3241 gatgctattg atttctataa ggcgtttaat ttcttcaaca acagaccaat tagataaagg
     3301 ggttactgta cagttttgcg atacacctct gccaatagga ttgtcattgc agtacatacc
     3361 tttatgacct ttatctttgg aactaccagt cattttatat cgacttggtt tcccttttac
     3421 atctttgaat ttgtgctgtt ctttcccaaa attacaacaa aacggattga tgcagtaatt
     3481 atattgaatg ggatattcag aaccgttcca tttaaaagac gttggcaaga acaacatttt
     3541 gttatacctt gttgcaaatc gttttggagt taataaatca tattgtttag ccctatcttt
     3601 taattcttcg tttgaaatcg gaattttaac atctacaatt tcatcttcct tcgtggctaa
     3661 tcttttaagc ttggtcattg tctatcaacc cactctatct ttgataccca ttttttcaac
     3721 aatccgctta ttacttcgct tgttggcaat ccattcaata aacttcttat cgtctaaaat
     3781 gttcaataag aattcatcca ctgcttcgct ttcagtacgc tctgcatatt cagcgtattg
     3841 cttaactatc acccttactt gctctgatat ttcccaatcc acagacttag cgttcttatt
     3901 ttttggctca agaaattgca taggaaaatg cacccctttt tgcttagtaa attgtaacca
     3961 ttttaacaca ccaatttgca ttcctacaat aattatttca cgttcgcatt tttatataca
     4021 ttttaactta cattttgtgg tgtaaattaa caacaaacgt tgatttaaca ataaaaaacg
     4081 agtatcaaat tcagttgcaa tttgatactc atttaggttt acattttcac tttttgtact
     4141 ccaaaaccga actacttaaa tctatactgc tgcctctttt ttttagtaca tgtccttcat
     4201 cttcgcagga agtgccaata tgattttaag aaaggtaata gacaaaaagt caaaaagata
     4261 aaacagagtt tcttctttac aaagggaatg tatgttcgta taatggtatt gaggtgaagg
     4321 ttatggagaa aaaatatgga acagtagata agatccgtac tttgcaagtt agtgccagac
     4381 cgttgatccg tttcagttta aatggggtca attgtttgat tgcttcccat agtttgaact
     4441 tcctagctga agtagacgaa gggatgaagt tagtcgttac aggttattat aataatagaa
     4501 agcaatttgt tgtgagatcc tatcatgtgt tagggaaacc caaaattgtg gtagaatatg
     4561 agaaaagtct atacccaaga aaaaaggtcc gacaagatga aagaataaac tagaaaacaa
     4621 gcggctgaga caaaaatgtc ctagccgctt gttttggaaa gatggatgca gacgatggga
     4681 tttgaaccca cacgagcata cgctcacaag aacctcaatc tatatataac tacatgtatt
     4741 taagtatttg tgatgaatca ctaaactttc caaactcatt tgagggatat tgcttaaaga
     4801 ttgaacagat caattcgaag ccctattagt attcgctcaa tggggggatc agaggccata
     4861 aaaagagact agtcattact agtctcaggt agtggaattg taggatttta aacgttcctg
     4921 gagagtaccg gtatgtaact cgaagaggtg attatcatag tcgtaaaagt aaattgaaga
     4981 agcctcgcct ttcacacgag aacgtcctgg ttcgacagtt aaacctaaca tttggatttt
     5041 tgagacaaac gaatctatat cttgttcgtc aattttaaaa gcaatatgat tgtatgtctt
     5101 tggcagttta ttcgacgaat tctgcattac tgcaatccat aggtccttaa cta
//
LOCUS       DAXWLG010000444         4586 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.183, whole genome shotgun sequence.
ACCESSION   DAXWLG010000444 DAXWLG010000000
VERSION     DAXWLG010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.183"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..846
                     /locus_tag="ACGE4T_002989"
     CDS             <1..846
                     /locus_tag="ACGE4T_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8388174.1"
                     /translation="VAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIYYNSESFDI
                     NTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIKRVEQIMKD
                     TEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETYPNALQKSL
                     VNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYCINEKKAIK
                     LLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINNFLNEESSD
                     ERKQI"
     gene            827..1561
                     /locus_tag="ACGE4T_002990"
     CDS             827..1561
                     /locus_tag="ACGE4T_002990"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8388175.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1594..2502
                     /locus_tag="ACGE4T_002991"
     CDS             1594..2502
                     /locus_tag="ACGE4T_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8388176.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2499..3041
                     /gene="sat4"
                     /locus_tag="ACGE4T_002992"
     CDS             2499..3041
                     /gene="sat4"
                     /locus_tag="ACGE4T_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8388177.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3134..3928
                     /locus_tag="ACGE4T_002993"
                     /note="aph(3')-IIIa"
     CDS             3134..3928
                     /locus_tag="ACGE4T_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8388178.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4204..4548
                     /locus_tag="ACGE4T_002994"
                     /pseudo
     CDS             4204..4548
                     /locus_tag="ACGE4T_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1561 a          686 c         1042 g         1297 t
ORIGIN      
        1 gtagcagaga aattatcctc tctgtcttat atagaaggta ttgttttagg tggttcacgt
       61 gcaaggggca cccatacaga ggattcggat atagatatcg gcatctatta caattcagaa
      121 tcatttgaca taaatactat taatcaattc gctacaaagc tggatgatga gcatagaaat
      181 aaccttgttg tacctcccgg agcatggggt gattggatta atggcggcgg atggttagtc
      241 ataaacgggt atcatgtgga tttaatttta cgtgatatta aacgtgtgga acaaataatg
      301 aaagatacag agcacggaat tgttactgcc aattatcaga ctgggcatcc ccatggttat
      361 attagtgcaa tgtatcgagg agaattagcg attagcaaaa tactatatgc taagaatgaa
      421 agcttatgcg aattaaaaaa acaggcagaa acttatccca atgctttgca gaaaagttta
      481 gttaactttt ttatgtttga agcagggttc tctttaatgt ttgtaaaagc aaattcggga
      541 acagacgata aatattatat tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa
      601 gtgttatttg catgtaataa tgcttattgt atcaacgaaa agaaagctat aaaactgctt
      661 gaaacttttg aacataaacc tgaaaaatat accgagaagg taaatcatat ttttgaagta
      721 ctcggtatct cactttttga atgctacgac atgaccgaga agctttataa tgaagtgaat
      781 gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa
      841 atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact
      901 ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg
      961 tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc
     1021 ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac
     1081 ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga
     1141 gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt
     1201 aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca
     1261 tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact
     1321 gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga
     1381 aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag
     1441 ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg
     1501 gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta
     1561 ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat
     1621 ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca
     1681 cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta
     1741 agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata
     1801 atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat
     1861 cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta
     1921 gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt
     1981 aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag
     2041 tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc
     2101 cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg
     2161 agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac
     2221 tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac
     2281 cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg
     2341 gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat
     2401 ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat
     2461 agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag
     2521 ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac
     2581 cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa
     2641 aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg
     2701 acaatactga taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta
     2761 aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt
     2821 tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata
     2881 aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct
     2941 atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     3001 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     3061 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     3121 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     3181 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     3241 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     3301 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     3361 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     3421 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     3481 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     3541 agacagccga ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     3601 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     3661 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     3721 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     3781 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     3841 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     3901 ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa aagctttaac
     3961 tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc catactctga
     4021 tgttttatat cttttctaaa agttcgctag atagagttct atattagaga tgtaaagagt
     4081 tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag aagcactaac
     4141 agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga ggaggaataa
     4201 aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc aaaaaaaata
     4261 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     4321 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     4381 cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt gggtgcattg
     4441 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     4501 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacgg ttctgttgca
     4561 aagttttaaa taaagaataa aatccc
//
LOCUS       DAXWLG010000445         3691 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.207, whole genome shotgun sequence.
ACCESSION   DAXWLG010000445 DAXWLG010000000
VERSION     DAXWLG010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.207"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..846
                     /locus_tag="ACGE4T_002995"
     CDS             <1..846
                     /locus_tag="ACGE4T_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8388179.1"
                     /translation="VAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIYYNSESFDI
                     NTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIKRVEQIMKD
                     TEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETYPNALQKSL
                     VNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYCINEKKAIK
                     LLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINNFLNEESSD
                     ERKQI"
     gene            827..1561
                     /locus_tag="ACGE4T_002996"
     CDS             827..1561
                     /locus_tag="ACGE4T_002996"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8388180.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1594..2502
                     /locus_tag="ACGE4T_002997"
     CDS             1594..2502
                     /locus_tag="ACGE4T_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8388181.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2499..3041
                     /gene="sat4"
                     /locus_tag="ACGE4T_002998"
     CDS             2499..3041
                     /gene="sat4"
                     /locus_tag="ACGE4T_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8388182.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3134..3691
                     /locus_tag="ACGE4T_002999"
                     /pseudo
     CDS             3134..3691
                     /locus_tag="ACGE4T_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1271 a          550 c          838 g         1032 t
ORIGIN      
        1 gtagcagaga aattatcctc tctgtcttat atagaaggta ttgttttagg tggttcacgt
       61 gcaaggggca cccatacaga ggattcggat atagatatcg gcatctatta caattcagaa
      121 tcatttgaca taaatactat taatcaattc gctacaaagc tggatgatga gcatagaaat
      181 aaccttgttg tacctcccgg agcatggggt gattggatta atggcggcgg atggttagtc
      241 ataaacgggt atcatgtgga tttaatttta cgtgatatta aacgtgtgga acaaataatg
      301 aaagatacag agcacggaat tgttactgcc aattatcaga ctgggcatcc ccatggttat
      361 attagtgcaa tgtatcgagg agaattagcg attagcaaaa tactatatgc taagaatgaa
      421 agcttatgcg aattaaaaaa acaggcagaa acttatccca atgctttgca gaaaagttta
      481 gttaactttt ttatgtttga agcagggttc tctttaatgt ttgtaaaagc aaattcggga
      541 acagacgata aatattatat tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa
      601 gtgttatttg catgtaataa tgcttattgt atcaacgaaa agaaagctat aaaactgctt
      661 gaaacttttg aacataaacc tgaaaaatat accgagaagg taaatcatat ttttgaagta
      721 ctcggtatct cactttttga atgctacgac atgaccgaga agctttataa tgaagtgaat
      781 gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa
      841 atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact
      901 ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg
      961 tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc
     1021 ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac
     1081 ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga
     1141 gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt
     1201 aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca
     1261 tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact
     1321 gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga
     1381 aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag
     1441 ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg
     1501 gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta
     1561 ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat
     1621 ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca
     1681 cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta
     1741 agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata
     1801 atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat
     1861 cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta
     1921 gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt
     1981 aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag
     2041 tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc
     2101 cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg
     2161 agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac
     2221 tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac
     2281 cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg
     2341 gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat
     2401 ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat
     2461 agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag
     2521 ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac
     2581 cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa
     2641 aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg
     2701 acaatactga taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta
     2761 aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt
     2821 tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata
     2881 aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct
     2941 atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     3001 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     3061 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     3121 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     3181 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     3241 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     3301 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     3361 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     3421 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     3481 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     3541 agacagccga ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     3601 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttaaaga
     3661 cggaaaagcc cgaagaggaa cttgtctttt c
//
LOCUS       DAXWLG010000446         3616 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.211, whole genome shotgun sequence.
ACCESSION   DAXWLG010000446 DAXWLG010000000
VERSION     DAXWLG010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.211"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            23..1144
                     /locus_tag="ACGE4T_003000"
     CDS             23..1144
                     /locus_tag="ACGE4T_003000"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8388183.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1402..2904
                     /gene="eat(A)"
                     /locus_tag="ACGE4T_003001"
     CDS             1402..2904
                     /gene="eat(A)"
                     /locus_tag="ACGE4T_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8388184.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2971..3498
                     /locus_tag="ACGE4T_003002"
     CDS             2971..3498
                     /locus_tag="ACGE4T_003002"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8388185.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1229 a          624 c          808 g          955 t
ORIGIN      
        1 aagaagaaag tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag
       61 tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag
      121 aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc
      181 gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat
      241 cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc
      301 attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta
      361 tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac
      421 tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc
      481 tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg
      541 aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata
      601 tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac
      661 gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga
      721 ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt
      781 tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga
      841 aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac
      901 gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat
      961 aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat
     1021 tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac
     1081 attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa
     1141 gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg
     1201 gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg
     1261 gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc
     1321 tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct
     1381 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
     1441 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
     1501 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
     1561 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
     1621 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
     1681 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
     1741 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
     1801 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
     1861 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
     1921 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
     1981 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
     2041 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
     2101 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
     2161 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
     2221 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
     2281 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
     2341 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
     2401 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
     2461 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag
     2521 ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg
     2581 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
     2641 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
     2701 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
     2761 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
     2821 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
     2881 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
     2941 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     3001 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
     3061 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     3121 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
     3181 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
     3241 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
     3301 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
     3361 gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat
     3421 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
     3481 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
     3541 cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac
     3601 ctttagaaat gaaatt
//
LOCUS       DAXWLG010000447         2555 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.236, whole genome shotgun sequence.
ACCESSION   DAXWLG010000447 DAXWLG010000000
VERSION     DAXWLG010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.236"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            <1..459
                     /locus_tag="ACGE4T_003003"
     CDS             <1..459
                     /locus_tag="ACGE4T_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8388186.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR-A"
                     /locus_tag="ACGE4T_003004"
     CDS             673..1368
                     /gene="vanR-A"
                     /locus_tag="ACGE4T_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8388187.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="ACGE4T_003005"
     CDS             1346..2500
                     /locus_tag="ACGE4T_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8388188.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          881 a          454 c          535 g          685 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa tctta
//
LOCUS       DAXWLG010000448         1196 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.328, whole genome shotgun sequence.
ACCESSION   DAXWLG010000448 DAXWLG010000000
VERSION     DAXWLG010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.328"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            38..775
                     /gene="erm(B)"
                     /locus_tag="ACGE4T_003006"
     CDS             38..775
                     /gene="erm(B)"
                     /locus_tag="ACGE4T_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8388189.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            945..1196
                     /locus_tag="ACGE4T_003007"
                     /pseudo
     CDS             945..1196
                     /locus_tag="ACGE4T_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          449 a          197 c          205 g          345 t
ORIGIN      
        1 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
       61 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      121 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      181 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      241 agaaaaatta aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca
      301 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      361 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      421 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      481 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      541 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      601 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      661 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      721 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
      781 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
      841 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
      901 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
      961 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1021 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1081 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1141 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttt
//
LOCUS       DAXWLG010000449         1071 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.337, whole genome shotgun sequence.
ACCESSION   DAXWLG010000449 DAXWLG010000000
VERSION     DAXWLG010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.337"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            27..>1071
                     /gene="tet"
                     /locus_tag="ACGE4T_003008"
     CDS             27..>1071
                     /gene="tet"
                     /locus_tag="ACGE4T_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HFF8388190.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTELGRSRPSSI"
BASE COUNT          305 a          161 c          220 g          385 t
ORIGIN      
        1 attaaagtgt aaaggaggaa ttaattatga agtgtaatga atgtaacagg gttaaataca
       61 accaaatttt aatttggctt tgcgttttat ctttttttag cgtattaaat gaaatggttt
      121 tgaacgtctc attacctgat attgcaaatg attttaataa accacctgcg agtacaaact
      181 gggtgaacac agcctttatg ttaacctttt ccattggaac agctgtatat ggaaagctat
      241 ctgatcaatt aggcatcaaa aggttactcc tatttggaat tataataaat tgtttcgggt
      301 cggtaattgg gtttgttggc cattctttct tttccttact tattatggct cgttttattc
      361 aaggggctgg tgcagctgca tttccagcac tcgtaatggt tgtagttgcg cgctatattc
      421 caaaggaaaa taggggtaaa gcatttggtc ttattggatc gatagtagcc atgggagaag
      481 gagtcggtcc agcgattggt ggaatgatag cccattatat tcattggtcc tatcttctac
      541 tcattcctat gataacaatt atcactgttc cgtttcttat gaaattatta aagaaagaag
      601 taaggataaa aggtcatttt gatatcaaag gaattatact aatgtctgta ggcattgtat
      661 tttttatgtt gtttacaaca tcatatagca tttcttttct tatcgttagc gtgctgtcat
      721 tcctgatatt tgtaaaacat atcaagaaag taacagatcc ttttgttgat cccggattag
      781 ggaaaaatat accttttatg attggagttc tttgtggggg aattatattt ggaacagtag
      841 cagggtttgt ctctatggtt ccttatatga tgaaagatgt tcaccagcta agtactgccg
      901 aaatcggaag tgtaattatt ttccctggaa caatgagtgt cattattttc ggctacattg
      961 gtgggatact tgttgataga agaggtcctt tatacgtgtt aaacatcgga gttacatttc
     1021 tttctgttag ctttttaaca gaattaggca gaagtcgccc atcctctata g
//
LOCUS       DAXWLG010000450          726 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id57
            SAMN40749881-rid21412713.guided.369, whole genome shotgun sequence.
ACCESSION   DAXWLG010000450 DAXWLG010000000
VERSION     DAXWLG010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749881
            Sequence Read Archive: SRR28555115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134725.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134725.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:56:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,009
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,869
            CDSs (with protein)               :: 2,869
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 36 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 18 of 78
            Pseudo Genes (multiple problems)  :: 9 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749881-rid21412713.guided.369"
                     /isolate="id57"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2023-09-18"
     gene            113..661
                     /gene="aac(6')"
                     /locus_tag="ACGE4T_003009"
     CDS             113..661
                     /gene="aac(6')"
                     /locus_tag="ACGE4T_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8388191.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          266 a          110 c          165 g          185 t
ORIGIN      
        1 gataaataat gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat
       61 ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat
      121 cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact
      181 gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc
      241 agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat
      301 ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg
      361 aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg
      421 aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac
      481 ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc
      541 gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg
      601 ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata
      661 ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa
      721 ttactt
//
